Science.gov

Sample records for biomass production potential

  1. Phytoplankton biomass, production and potential export in the North Water

    NASA Astrophysics Data System (ADS)

    Klein, Bert; LeBlanc, Bernard; Mei, Zhi-Ping; Beret, Rachel; Michaud, Josée; Mundy, C.-J.; von Quillfeldt, Cecilie H.; Garneau, Marie-Ève; Roy, Suzanne; Gratton, Yves; Cochran, J. Kirk; Bélanger, Simon; Larouche, Pierre; Pakulski, J. Dean; Rivkin, Richard B.; Legendre, Louis

    The seasonal patterns of phytoplankton biomass and production were determined in the North Water, located between Greenland and Ellesmere Island (Canadian Arctic), in August 1997, April-July 1998, and August-September 1999. The patterns differed among the four defined regions of this large polynya, i.e. North (>77.5°N), East (>75°W), West (<75°W), and South (<76°N). Phytoplankton biomass and production were low during April throughout the North Water. Biomass first increased in the East during April. From there, the biomass spread north- and westwards during May-June, when the bloom culminated (chlorophyll a concentrations up to 19.8 mg m -3). The large-sized (>5 μm) fraction dominated the biomass and production during the bloom. During July, August, and September, biomass and production decreased over the whole region, with the highest biomass, dominated by large cells, occurring in the North. The annual particulate and dissolved phytoplankton production were the highest ever reported for the high Arctic, reaching maximum values of 254 and 123 g C m -2 yr -1, respectively, in the East. Rates in the North and West were considerably lower than in the East (ca. two- and three-fold, respectively). The f-ratios (i.e. ratio of new to total production), derived from the size structure of phytoplankton, were high north of 76°N (0.4-0.7). Regionally, this indicated a high potential export of particulate organic carbon ( EPOC) from the phytoplankton community to other trophic compartments and/or downwards in the East (155 g C m -2 yr -1), with lower values in the North and West (i.e. 77 and 42 g C m -2 yr -1, respectively). The seasonal and spatial patterns of EPOC were consistent with independent estimates of potential carbon export. Phytoplankton biomass and production were generally dominated by the large size fraction, whereas EPOC seemed to be dominated by the large size fraction early in the season and by the small size fraction (<5 μm) from June until the end

  2. Ozone production potential following convective redistribution of biomass burning emissions

    NASA Technical Reports Server (NTRS)

    Pickering, Kenneth E.; Thompson, Anne M.; Scala, John R.; Tao, Wei-Kuo; Simpson, Joanne

    1992-01-01

    The effects of deep convection on the potential for forming ozone in the free troposphere have been simulated for regions where the trace gas composition is influenced by biomass burning. Cloud photochemical and dynamic simulations based on observations in the 1980 and 1985 Brazilian campaigns form the basis of a sensitivity study of the ozone production potential under differing conditions. It is seen that there is considerably more ozone formed in the middle and upper troposphere when convection has redistributed hydrocarbons, NO(x), and CO compared to the example of no convection.

  3. Bioenergy potential of Ulva lactuca: biomass yield, methane production and combustion.

    PubMed

    Bruhn, Annette; Dahl, Jonas; Nielsen, Henrik Bangsø; Nikolaisen, Lars; Rasmussen, Michael Bo; Markager, Stiig; Olesen, Birgit; Arias, Carlos; Jensen, Peter Daugbjerg

    2011-02-01

    The biomass production potential at temperate latitudes (56°N), and the quality of the biomass for energy production (anaerobic digestion to methane and direct combustion) were investigated for the green macroalgae, Ulva lactuca. The algae were cultivated in a land based facility demonstrating a production potential of 45T (TS) ha(-1) y(-1). Biogas production from fresh and macerated U. lactuca yielded up to 271 ml CH(4) g(-1) VS, which is in the range of the methane production from cattle manure and land based energy crops, such as grass-clover. Drying of the biomass resulted in a 5-9-fold increase in weight specific methane production compared to wet biomass. Ash and alkali contents are the main challenges in the use of U. lactuca for direct combustion. Application of a bio-refinery concept could increase the economical value of the U. lactuca biomass as well as improve its suitability for production of bioenergy. PMID:21044839

  4. Sources of biomass feedstock variability and the potential impact on biofuels production

    SciTech Connect

    Williams, C. Luke; Westover, Tyler L.; Emerson, Rachel M.; Tumuluru, Jaya Shankar; Li, Chenlin

    2015-11-23

    In this study, terrestrial lignocellulosic biomass has the potential to be a carbon neutral and domestic source of fuels and chemicals. However, the innate variability of biomass resources, such as herbaceous and woody materials, and the inconsistency within a single resource due to disparate growth and harvesting conditions, presents challenges for downstream processes which often require materials that are physically and chemically consistent. Intrinsic biomass characteristics, including moisture content, carbohydrate and ash compositions, bulk density, and particle size/shape distributions are highly variable and can impact the economics of transforming biomass into value-added products. For instance, ash content increases by an order of magnitude between woody and herbaceous feedstocks (from ~0.5 to 5 %, respectively) while lignin content drops by a factor of two (from ~30 to 15 %, respectively). This increase in ash and reduction in lignin leads to biofuel conversion consequences, such as reduced pyrolysis oil yields for herbaceous products as compared to woody material. In this review, the sources of variability for key biomass characteristics are presented for multiple types of biomass. Additionally, this review investigates the major impacts of the variability in biomass composition on four conversion processes: fermentation, hydrothermal liquefaction, pyrolysis, and direct combustion. Finally, future research processes aimed at reducing the detrimental impacts of biomass variability on conversion to fuels and chemicals are proposed.

  5. Sources of biomass feedstock variability and the potential impact on biofuels production

    DOE PAGESBeta

    Williams, C. Luke; Westover, Tyler L.; Emerson, Rachel M.; Tumuluru, Jaya Shankar; Li, Chenlin

    2015-11-23

    In this study, terrestrial lignocellulosic biomass has the potential to be a carbon neutral and domestic source of fuels and chemicals. However, the innate variability of biomass resources, such as herbaceous and woody materials, and the inconsistency within a single resource due to disparate growth and harvesting conditions, presents challenges for downstream processes which often require materials that are physically and chemically consistent. Intrinsic biomass characteristics, including moisture content, carbohydrate and ash compositions, bulk density, and particle size/shape distributions are highly variable and can impact the economics of transforming biomass into value-added products. For instance, ash content increases by anmore » order of magnitude between woody and herbaceous feedstocks (from ~0.5 to 5 %, respectively) while lignin content drops by a factor of two (from ~30 to 15 %, respectively). This increase in ash and reduction in lignin leads to biofuel conversion consequences, such as reduced pyrolysis oil yields for herbaceous products as compared to woody material. In this review, the sources of variability for key biomass characteristics are presented for multiple types of biomass. Additionally, this review investigates the major impacts of the variability in biomass composition on four conversion processes: fermentation, hydrothermal liquefaction, pyrolysis, and direct combustion. Finally, future research processes aimed at reducing the detrimental impacts of biomass variability on conversion to fuels and chemicals are proposed.« less

  6. Potential effects on grassland birds of converting marginal cropland to switchgrass biomass production

    USGS Publications Warehouse

    Murray, L.D.; Best, L.B.; Jacobsen, T.J.; Braster, M.L.

    2003-01-01

    Habitat loss is a major reason for the decline of grassland birds in North America. Five habitats (pastures, hayfields, rowcrop fields, small-grain fields, Conservation Reserve Program fields) compose most of the habitat used by grassland birds in the Midwest United States. Growing and harvesting switchgrass (Panicum virgatum) as a biomass fuel would create another habitat for grassland birds. Bird abundance information from studies conducted in Iowa and adjacent states and land-use data for the Rathbun Lake Watershed in southern Iowa were used in a Geographic Information System to model the potential effects on bird abundances of converting rowcrop fields to biomass production. Abundances of bird species that are management priorities increased in both biomass scenarios. Common yellowthroat (Geothlypis trichas) abundance in the watershed also increased greatly in both scenarios. Other species (e.g., homed lark [Eremophila alpestris], killdeer [Charadrius vociferous]) were more abundant in the existing land use than in the biomass scenarios, and conversion of fields from rowcrop to biomass production could be detrimental to these species. In general, biomass fields will provide habitat for grassland birds that are management priorities, but future monitoring of birds in such fields is needed as conversion of rowcrop fields to biomass production continues. ?? 2002 Elsevier Science Ltd. All rights reserved.

  7. Three types of Marine microalgae and Nannocholoropsis oculata cultivation for potential source of biomass production

    NASA Astrophysics Data System (ADS)

    Krishnan, Vijendren; Uemura, Yoshimitsu; Tien Thanh, Nguyen; Khalid, Nadila Abdul; Osman, Noridah; Mansor, Nurlidia

    2015-06-01

    Microalgae have been vastly investigated throughout the world for possible replacement of fossil fuels, besides utilization in remediation of leachate, disposal of hypersaline effluent and also as feedstock for marine organisms. This research particularly has focused on locally available marine microalgae sample and Nannochloropsis oculata for potential mass production of microalgae biomass. Biomass produced by sample 1 and sample 2 is 0.6200 g/L and 0.6450 g/L respectively. Meanwhile, sample 3 and N. oculata has obtained maximum biomass concentration of 0.4917 g/L and 0.5183 g/L respectively. This shows that sample 1 and sample 2 has produced approximately 20% higher biomass concentration in comparison to sample 3 and N. oculata. Although sample 3 and N. oculata is slightly lower than other samples, the maximum biomass was achieved four days earlier. Hence, the specific growth rate of sample 3 and N. oculata is higher; meanwhile the specific growth rate of N. oculata is the highest. Optical density measurements of all the sample throughout the cultivation period also correlates well with the biomass concentration of microalgae. Therefore, N. oculata is finally selected for utilization in mass production of microalgae biomass.

  8. A comprehensive review of biomass resources and biofuel production in Nigeria: potential and prospects.

    PubMed

    Sokan-Adeaga, Adewale Allen; Ana, Godson R E E

    2015-01-01

    The quest for biofuels in Nigeria, no doubt, represents a legitimate ambition. This is so because the focus on biofuel production has assumed a global dimension, and the benefits that may accrue from such effort may turn out to be enormous if the preconditions are adequately satisfied. As a member of the global community, it has become exigent for Nigeria to explore other potential means of bettering her already impoverished economy. Biomass is the major energy source in Nigeria, contributing about 78% of Nigeria's primary energy supply. In this paper, a comprehensive review of the potential of biomass resources and biofuel production in Nigeria is given. The study adopted a desk review of existing literatures on major energy crops produced in Nigeria. A brief description of the current biofuel developmental activities in the country is also given. A variety of biomass resources exist in the country in large quantities with opportunities for expansion. Biomass resources considered include agricultural crops, agricultural crop residues, forestry resources, municipal solid waste, and animal waste. However, the prospects of achieving this giant stride appear not to be feasible in Nigeria. Although the focus on biofuel production may be a worthwhile endeavor in view of Nigeria's development woes, the paper argues that because Nigeria is yet to adequately satisfy the preconditions for such program, the effort may be designed to fail after all. To avoid this, the government must address key areas of concern such as food insecurity, environmental crisis, and blatant corruption in all quarters. It is concluded that given the large availability of biomass resources in Nigeria, there is immense potential for biofuel production from these biomass resources. With the very high potential for biofuel production, the governments as well as private investors are therefore encouraged to take practical steps toward investing in agriculture for the production of energy crops and the

  9. Productivity ranges of sustainable biomass potentials from non-agricultural land

    NASA Astrophysics Data System (ADS)

    Schueler, Vivian; Fuss, Sabine; Steckel, Jan Christoph; Weddige, Ulf; Beringer, Tim

    2016-07-01

    Land is under pressure from a number of demands, including the need for increased supplies of bioenergy. While bioenergy is an important ingredient in many pathways compatible with reaching the 2 °C target, areas where cultivation of the biomass feedstock would be most productive appear to co-host other important ecosystems services. We categorize global geo-data on land availability into productivity deciles, and provide a geographically explicit assessment of potentials that are concurrent with EU sustainability criteria. The deciles unambiguously classify the global productivity range of potential land currently not in agricultural production for biomass cultivation. Results show that 53 exajoule (EJ) sustainable biomass potential are available from 167 million hectares (Mha) with a productivity above 10 tons of dry matter per hectare and year (tD Mha‑1 a‑1), while additional 33 EJ are available on 264 Mha with yields between 4 and 10 tD M ha‑1 a‑1: some regions lose less of their highly productive potentials to sustainability concerns than others and regional contributions to bioenergy potentials shift when less productive land is considered. Challenges to limit developments to the exploitation of sustainable potentials arise in Latin America, Africa and Developing Asia, while new opportunities emerge for Transition Economies and OECD countries to cultivate marginal land.

  10. Effects of light availability on the biomass production, CO2 fixation, and bioethanol production potential of Thermosynechococcus CL-1.

    PubMed

    Su, Chih Ming; Hsueh, Hsin Ta; Li, Tzu Ying; Huang, Li Che; Chu, Yi Ling; Tseng, Chi Ming; Chu, Hsin

    2013-10-01

    A thermophilic cyanobacterium named Thermosynechococcus CL-1 (TCL-1) was cultivated in this study to eliminate the energy input of cooling system in flat plate photobioreactors. Cultivating TCL-1 in the 1.5 cm light path flat plate photobioreactor exhibited stable characteristics for biomass production, CO2 fixation, and carbohydrate production under high illumination conditions (1000 or 2000 μE m(-2) s(-1)). The greatest biomass and carbohydrate productivity, and CO2 fixation rate were recorded at 116, 67, and 170 mg/L/h, respectively, in the 1.5c m light path photobioreactor and under optimal biomass concentration (about 3 g/L). Cultivating Thermosynechococcus CL-1 in flat plate photobioreactors exhibits high potential for biomass production, CO2 fixation and bioethanol production. PMID:23545071

  11. Chemicals from biomass: an assessment of the potential for production of chemical feedstocks from renewable resources

    SciTech Connect

    Donaldson, T.L.; Culberson, O.L.

    1983-06-01

    This assessment of the potential for production of commodity chemicals from renewable biomass resources is based on (1) a Delphi study with 50 recognized authorities to identify key technical issues relevant to production of chemicals from biomass, and (2) a systems model based on linear programming for a commodity chemicals industry using renewable resources and coal as well as gas and petroleum-derived resources. Results from both parts of the assessment indicate that, in the absence of gas and petroleum, coal undoubtedly would be a major source of chemicals first, followed by biomass. The most attractive biomass resources are wood, agricultural residues, and sugar and starch crops. A reasonable approximation to the current product slate for the petrochemical industry could be manufactured using only renewable resources for feedstocks. Approximately 2.5 quads (10/sup 15/ Btu (1.055 x 10/sup 18/ joules)) per year of oil and gas would be released. Further use of biomass fuels in the industry could release up to an additional 1.5 quads. however, such an industry would be unprofitable under current economic conditions with existing or near-commercial technology. As fossil resources become more expensive and biotechnology becomes more efficient, the economics will be more favorable. Use of the chemicals industry model to evaluate process technologies is demonstrated. Processes are identified which have potential for significant added value to the system if process improvements can be made to improve the economics. Guidelines and recommendations for research and development programs to improve the attractiveness of chemicals from biomass are discussed.

  12. Evaluating the composition and processing potential of novel sources of Brazilian biomass for sustainable biorenewables production

    PubMed Central

    2014-01-01

    Background The search for promising and renewable sources of carbohydrates for the production of biofuels and other biorenewables has been stimulated by an increase in global energy demand in the face of growing concern over greenhouse gas emissions and fuel security. In particular, interest has focused on non-food lignocellulosic biomass as a potential source of abundant and sustainable feedstock for biorefineries. Here we investigate the potential of three Brazilian grasses (Panicum maximum, Pennisetum purpureum and Brachiaria brizantha), as well as bark residues from the harvesting of two commercial Eucalyptus clones (E. grandis and E. grandis x urophylla) for biofuel production, and compare these to sugarcane bagasse. The effects of hot water, acid, alkaline and sulfite pretreatments (at increasing temperatures) on the chemical composition, morphology and saccharification yields of these different biomass types were evaluated. Results The average yield (per hectare), availability and general composition of all five biomasses were compared. Compositional analyses indicate a high level of hemicellulose and lignin removal in all grass varieties (including sugarcane bagasse) after acid and alkaline pretreatment with increasing temperatures, whilst the biomasses pretreated with hot water or sulfite showed little variation from the control. For all biomasses, higher cellulose enrichment resulted from treatment with sodium hydroxide at 130°C. At 180°C, a decrease in cellulose content was observed, which is associated with high amorphous cellulose removal and 5-hydroxymethyl-furaldehyde production. Morphological analysis showed the effects of different pretreatments on the biomass surface, revealing a high production of microfibrillated cellulose on grass surfaces, after treatment with 1% sodium hydroxide at 130°C for 30 minutes. This may explain the higher hydrolysis yields resulting from these pretreatments, since these cellulosic nanoparticles can be easily

  13. Global biomass production potentials exceed expected future demand without the need for cropland expansion

    PubMed Central

    Mauser, Wolfram; Klepper, Gernot; Zabel, Florian; Delzeit, Ruth; Hank, Tobias; Putzenlechner, Birgitta; Calzadilla, Alvaro

    2015-01-01

    Global biomass demand is expected to roughly double between 2005 and 2050. Current studies suggest that agricultural intensification through optimally managed crops on today's cropland alone is insufficient to satisfy future demand. In practice though, improving crop growth management through better technology and knowledge almost inevitably goes along with (1) improving farm management with increased cropping intensity and more annual harvests where feasible and (2) an economically more efficient spatial allocation of crops which maximizes farmers' profit. By explicitly considering these two factors we show that, without expansion of cropland, today's global biomass potentials substantially exceed previous estimates and even 2050s' demands. We attribute 39% increase in estimated global production potentials to increasing cropping intensities and 30% to the spatial reallocation of crops to their profit-maximizing locations. The additional potentials would make cropland expansion redundant. Their geographic distribution points at possible hotspots for future intensification. PMID:26558436

  14. The potential impacts of biomass feedstock production on water resource availability.

    PubMed

    Stone, K C; Hunt, P G; Cantrell, K B; Ro, K S

    2010-03-01

    Biofuels are a major topic of global interest and technology development. Whereas bioenergy crop production is highly dependent on water, bioenergy development requires effective allocation and management of water. The objectives of this investigation were to assess the bioenergy production relative to the impacts on water resource related factors: (1) climate and weather impact on water supplies for biomass production; (2) water use for major bioenergy crop production; and (3) potential alternatives to improve water supplies for bioenergy. Shifts to alternative bioenergy crops with greater water demand may produce unintended consequences for both water resources and energy feedstocks. Sugarcane and corn require 458 and 2036 m(3) water/m(3) ethanol produced, respectively. The water requirements for corn grain production to meet the US-DOE Billion-Ton Vision may increase approximately 6-fold from 8.6 to 50.1 km(3). Furthermore, climate change is impacting water resources throughout the world. In the western US, runoff from snowmelt is occurring earlier altering the timing of water availability. Weather extremes, both drought and flooding, have occurred more frequently over the last 30 years than the previous 100 years. All of these weather events impact bioenergy crop production. These events may be partially mitigated by alternative water management systems that offer potential for more effective water use and conservation. A few potential alternatives include controlled drainage and new next-generation livestock waste treatment systems. Controlled drainage can increase water available to plants and simultaneously improve water quality. New livestock waste treatments systems offer the potential to utilize treated wastewater to produce bioenergy crops. New technologies for cellulosic biomass conversion via thermochemical conversion offer the potential for using more diverse feedstocks with dramatically reduced water requirements. The development of bioenergy

  15. Biomass production of multipopulation microalgae in open air pond for biofuel potential.

    PubMed

    Selvakumar, P; Umadevi, K

    2016-04-01

    Biodiesel gains attention as it is made from renewable resources and has considerable environmental benefits. The present investigation has focused on large scale cultivation of multipopulation microalgae in open air pond using natural sea water without any additional nutritive supplements for low cost biomass production as a possible source of biofuel in large scale. Open air algal pond attained average chlorophyll concentration of 11.01 µg/L with the maximum of 43.65 µg/L as well as a higher lipid concentration of 18% (w/w) with lipid content 9.3 mg/L on the 10th day of the culture; and maximum biomass of 0.36 g/L on the 7th day of the culture. Composition analysis of fatty acid methyl ester (FAME) was performed by gas chromatography and mass spectrometry (GCMS). Multipopulation of algal biomass had 18% of total lipid content with 55% of total saturated fatty acids (SFA), 35.3% of monounsaturated fatty acids (MUFA) and 9.7% of polyunsaturated fatty acids (PUFA), revealing a potential source of biofuel production at low cost. PMID:27295924

  16. Estimation of potential biomass resource and biogas production from aquatic plants in Argentina

    NASA Astrophysics Data System (ADS)

    Fitzsimons, R. E.; Laurino, C. N.; Vallejos, R. H.

    1982-08-01

    The use of aquatic plants in artificial lakes as a biomass source for biogas and fertilizer production through anaerobic fermentation is evaluated, and the magnitude of this resource and the potential production of biogas and fertilizer are estimated. The specific case considered is the artificial lake that will be created by the construction of Parana Medio Hydroelectric Project on the middle Parana River in Argentina. The growth of the main aquatic plant, water hyacinth, on the middle Parana River has been measured, and its conversion to methane by anaerobic fermentation is determined. It is estimated that gross methane production may be between 1.0-4.1 x 10 to the 9th cu cm/year. The fermentation residue can be used as a soil conditioner, and it is estimated production of the residue may represent between 54,900-221,400 tons of nitrogen/year, a value which is 2-8 times the present nitrogen fertilizer demand in Argentina.

  17. Pleurotus biomass production on vinasse and its potential use for aquaculture feed

    PubMed Central

    Sartori, S.B.; Ferreira, L.F.R.; Messias, T.G.; Souza, G.; Pompeu, G.B.; Monteiro, R.T.R.

    2015-01-01

    The vinasse is a by-product generated during the manufacture of alcohol from sugarcane fermentation. Rich in organic matter, it is known that the vinasse has the potential to be used as a source of nutrients for plants as well as microorganisms. In this study, the fungi Pleurotus sajor-caju, P. ostreatus, P. albidus and P. flabellatus were cultivated in vinasse and utilised as a complementary diet for Danio rerio fish. The fungi mycelia cultured in vinasse for 15 days were lyophilised and offered to the fishes at a rate of 2% (medium/body weight) for 28 days. P. albidus produced the highest biomass (16.27 g L−1). Bromatological analysis of mycelia showed similar values to commercial rations. Toxicity tests showed that fish survival was 100% and no significant biomass loss was observed, indicating that the tested fungi grown in vinasse showed no toxicity. Our results showed that vinasse is a promising by-product for fungal growth and the mycelia of Pleurotus sp. fungi can be included in the diets of fish as a nutritional supplement. PMID:26000196

  18. Pretreated densified biomass products

    SciTech Connect

    Dale, Bruce E; Ritchie, Bryan; Marshall, Derek

    2014-03-18

    A product comprising at least one densified biomass particulate of a given mass having no added binder and comprised of a plurality of lignin-coated plant biomass fibers is provided, wherein the at least one densified biomass particulate has an intrinsic density substantially equivalent to a binder-containing densified biomass particulate of the same given mass and h a substantially smooth, non-flakey outer surface. Methods for using and making the product are also described.

  19. Production of liquid fuels out of plant biomass and refuse: Methods, cost, potential

    SciTech Connect

    Woick, B.; Friedrich, R.

    1981-09-01

    Different ways of producing biomass and its conversion into high grade fuel for vehicles are reviewed with particular reference to physical and geographical factors, pertaining in the Federal Republic of Germany (FRG). Even with the potentially small amount of biomass in the FRG, the fueling of diesel engines with rape oil or modified ethanol, which can be obtained from any cellulosic feedstock, seems to pose the fewest difficulties and promises greatest efficiency. However, the amount of fuel produced from biomass can probably only meet a very small percentage of the total amount required.

  20. Characterization of Nizimuddinia zanardini macroalgae biomass composition and its potential for biofuel production.

    PubMed

    Yazdani, Parviz; Zamani, Akram; Karimi, Keikhosro; Taherzadeh, Mohammad J

    2015-01-01

    Nizimuddinia zanardini macroalgae, harvested from Persian Gulf, was chemically characterized and employed for the production of ethanol, seaweed extract, alginic acid, and biogas. In order to improve the products yields, the biomass was pretreated with dilute sulfuric acid and hot water. The pretreated and untreated biomasses were subjected to enzymatic hydrolysis by cellulase (15FPU/g) and β-glucosidase (30IU/g). Hydrolysis yield of glucan was 29.8, 82.5, and 72.7g/kg for the untreated, hot-water pretreated, and acid pretreated biomass, respectively. Anaerobic fermentation of hydrolysates by Saccharomycescerevisiae resulted in the maximum ethanol yield of 34.6g/kg of the dried biomass. A seaweed extract containing mannitol and a solid residue containing alginic acid were recovered as the main byproducts of the ethanol production. On the other hand, the biogas yield from the biomass was increased from 170 to 200m(3) per ton of dried algae biomass by hot water pretreatment. PMID:25461003

  1. The Potential for Biomass District Energy Production in Port Graham, Alaska

    SciTech Connect

    Charles Sink, Chugachmiut; Keeryanne Leroux, EERC

    2008-05-08

    This project was a collaboration between The Energy & Environmental Research Center (EERC) and Chugachmiut – A Tribal organization Serving the Chugach Native People of Alaska and funded by the U.S. Department of Energy (DOE) Tribal Energy Program. It was conducted to determine the economic and technical feasibility for implementing a biomass energy system to service the Chugachmiut community of Port Graham, Alaska. The Port Graham tribe has been investigating opportunities to reduce energy costs and reliance on energy imports and support subsistence. The dramatic rise in the prices of petroleum fuels have been a hardship to the village of Port Graham, located on the Kenai Peninsula of Alaska. The Port Graham Village Council views the forest timber surrounding the village and the established salmon industry as potential resources for providing biomass energy power to the facilities in their community. Benefits of implementing a biomass fuel include reduced energy costs, energy independence, economic development, and environmental improvement. Fish oil–diesel blended fuel and indoor wood boilers are the most economical and technically viable options for biomass energy in the village of Port Graham. Sufficient regional biomass resources allow up to 50% in annual heating savings to the user, displacing up to 70% current diesel imports, with a simple payback of less than 3 years for an estimated capital investment under $300,000. Distributive energy options are also economically viable and would displace all imported diesel, albeit offering less savings potential and requiring greater capital. These include a large-scale wood combustion system to provide heat to the entire village, a wood gasification system for cogeneration of heat and power, and moderate outdoor wood furnaces providing heat to 3–4 homes or community buildings per furnace. Coordination of biomass procurement and delivery, ensuring resource reliability and technology acceptance, and arbitrating

  2. Forecasting fish biomasses, densities, productions, and bioaccumulation potentials of mid-atlantic wadeable streams.

    PubMed

    Barber, M Craig; Rashleigh, Brenda; Cyterski, Michael J

    2016-01-01

    Regional fishery conditions of Mid-Atlantic wadeable streams in the eastern United States are estimated using the Bioaccumulation and Aquatic System Simulator (BASS) bioaccumulation and fish community model and data collected by the US Environmental Protection Agency's Environmental Monitoring and Assessment Program (EMAP). Average annual biomasses and population densities and annual productions are estimated for 352 randomly selected streams. Realized bioaccumulation factors (BAF) and biomagnification factors (BMF), which are dependent on these forecasted biomasses, population densities, and productions, are also estimated by assuming constant water exposures to methylmercury and tetra-, penta-, hexa-, and hepta-chlorinated biphenyls. Using observed biomasses, observed densities, and estimated annual productions of total fish from 3 regions assumed to support healthy fisheries as benchmarks (eastern Tennessee and Catskill Mountain trout streams and Ozark Mountains smallmouth bass streams), 58% of the region's wadeable streams are estimated to be in marginal or poor condition (i.e., not healthy). Using simulated BAFs and EMAP Hg fish concentrations, we also estimate that approximately 24% of the game fish and subsistence fishing species that are found in streams having detectable Hg concentrations would exceed an acceptable human consumption criterion of 0.185 μg/g wet wt. Importantly, such streams have been estimated to represent 78.2% to 84.4% of the Mid-Atlantic's wadeable stream lengths. Our results demonstrate how a dynamic simulation model can support regional assessment and trends analysis for fisheries. PMID:25858149

  3. Measurement of the inhibitory potential and detoxification of biomass pretreatment hydrolysate for ethanol production

    SciTech Connect

    Rivard, C.J.; Engel, R.E.; Nagle, N.J.

    1996-12-31

    The Microtox assay represents a rapid, accurate, and reproducible method for determining general microbial toxicity. This assay was used to evaluate the relative toxicity of a variety of hydrolysate samples derived from dilute-acid and alkaline biomass pretreatment. Toxicity is elicited from biomass degradation products, such as furfural, hydroxymethyl furfural, and acetic acid, generated during pretreatment. Microtox results indicate that the pretreatment samples examined ranged from 9 to 71 toxicity units (TU). Correlations of TU and sample absorbance at several wavelengths were evaluated for all sample series. Sample TU values best agreed with absorbance at 230 nm, but the unsatisfactory fit suggests that absorbance should not be used as an absolute measure of sample toxicity. Microtox data for pretreatment hydrolysate samples were correlated with the inhibition experienced by the ethanologenic yeast, Saccharomyces cerevisiae strain D{sub 5}A, during the simultaneous saccharification and fermentation (SSF) process of pretreated biomass. None of the alkaline pretreatment conditions produced inhibition during SSF. However, the acid pretreatment conditions did produce a wide range of inhibitory and noninhibitory hydrolysates. In general, fermentation was inhibited for acid-pretreated hydrolysate samples with values exceeding 45 TU. Preliminary studies that focused on reducing hydrolysate sample toxicity (detoxification) indicate that adding perlite and zeolite had little effect. However, the use of charcoal, a universal flocculent, or ion-exchange resins significantly reduced sample toxicity, holding promise for the efficient bioconversion of pretreated biomass to ethanol. Moreover, the developed toxicity measurement assay can quickly monitor the quality of the pretreatment process. In this way, biomass conversion operation processes can be reliably controlled at the pilot and commercial scales. 4 refs., 4 figs., 3 tabs.

  4. Biomass production in Florida

    SciTech Connect

    Smith, W.H.; Dowd, M.L.

    1981-08-01

    Florida posseses climatic, land, and water resources favorable for abundant biomass production. Therefore, a statewide program has been initiated to determine adapted species for the available array of production sites. Plant resources under investigation include woody, aquatic, grasses, hydrocarbon, and root crop species. The goal is to produce a continuous stream of biomass for the various biofuel conversion options. Preliminary yields from energy cropping experiments range from about 10 to nearly 90 metric tons per hectare per year, depending on the crop and the production systems employed. (Refs. 15).

  5. Culture media optimization of Porphyridium purpureum: production potential of biomass, total lipids, arachidonic and eicosapentaenoic acid.

    PubMed

    Kavitha, Mysore Doddaiah; Kathiresan, Shanmugam; Bhattacharya, Sila; Sarada, Ravi

    2016-05-01

    Porphyridium purpureum a red marine microalga is known for phycobiliproteins (PB), polyunsaturated fatty acids and sulphated exopolysaccharides. In the present study, effects of media constituents for the production of different polyunsaturated fatty acids from P. purpureum were considered using a response surface methodology (RSM). A second order polynomial was used to predict the response functions in terms of the independent variables such as the concentrations of sodium chloride, magnesium sulphate, sodium nitrate and potassium dihydrogen phosphate. The response functions were production of biomass yield, total lipid and polyunsaturated fatty acids like arachidonic acid (AA 20:4) and eicosapentaenoic acid (EPA 20:5). Results corroborated that maximum Biomass (0.95 gL(-1)) yield was at the concentrations of sodium chloride (14.89 gL(-1)), magnesium sulfate (3.93 gL(-1)) and sodium nitrate (0.96 gL(-1)) and potassium dihydrogen phosphate (0.09 gL(-1)). Optimum total lipid (17.9 % w/w) and EPA (34.6 % w/w) content was at the concentrations of sodium chloride (29.98 gL(-1)), magnesium sulfate (9.34 gL(-1)) and sodium nitrate (1.86 gL(-1)). Variation in concentration of potassium dihydrogen phosphate for both lipid (0.01gL(-1)) and EPA content (0.20 gL(-1)) was observed. The optimum conditions for biomass, total lipid, AA and EPA varied indicating their batch mode of growth and interaction effect of the salt. PMID:27407193

  6. Banana by-products: an under-utilized renewable food biomass with great potential.

    PubMed

    Padam, Birdie Scott; Tin, Hoe Seng; Chye, Fook Yee; Abdullah, Mohd Ismail

    2014-12-01

    Banana (Musaceae) is one of the world's most important fruit crops that is widely cultivated in tropical countries for its valuable applications in food industry. Its enormous by-products are an excellent source of highly valuable raw materials for other industries by recycling agricultural waste. This prevents an ultimate loss of huge amount of untapped biomass and environmental issues. This review discusses extensively the breakthrough in the utilization of banana by-products such as peels, leaves, pseudostem, stalk and inflorescence in various food and non-food applications serving as thickening agent, coloring and flavor, alternative source for macro and micronutrients, nutraceuticals, livestock feed, natural fibers, and sources of natural bioactive compounds and bio-fertilizers. Future prospects and challenges are the important key factors discussed in association to the sustainability and feasibility of utilizing these by-products. It is important that all available by-products be turned into highly commercial outputs in order to sustain this renewable resource and provide additional income to small scale farming industries without compromising its quality and safety in competing with other commercial products. PMID:25477622

  7. The potential of freshwater macroalgae as a biofuels feedstock and the influence of nutrient availability on freshwater macroalgal biomass production

    NASA Astrophysics Data System (ADS)

    Yun, Jin-Ho

    Extensive efforts have been made to evaluate the potential of microalgae as a biofuel feedstock during the past 4-5 decades. However, filamentous freshwater macroalgae have numerous characteristics that favor their potential use as an alternative algal feedstock for biofuels production. Freshwater macroalgae exhibit high rates of areal productivity, and their tendency to form dense floating mats on the water surface imply significant reductions in harvesting and dewater costs compared to microalgae. In Chapter 1, I reviewed the published literature on the elemental composition and energy content of five genera of freshwater macroalgae. This review suggested that freshwater macroalgae compare favorably with traditional bio-based energy sources, including terrestrial residues, wood, and coal. In addition, I performed a semi-continuous culture experiment using the common Chlorophyte genus Oedogonium to investigate whether nutrient availability can influence its higher heating value (HHV), productivity, and proximate analysis. The experimental study suggested that the most nutrient-limited growth conditions resulted in a significant increase in the HHV of the Oedogonium biomass (14.4 MJ/kg to 16.1 MJ/kg). Although there was no significant difference in productivity between the treatments, the average dry weight productivity of Oedogonium (3.37 g/m2/day) was found to be much higher than is achievable with common terrestrial plant crops. Although filamentous freshwater macroalgae, therefore, have significant potential as a renewable source of bioenergy, the ultimate success of freshwater macroalgae as a biofuel feedstock will depend upon the ability to produce biomass at the commercial-scale in a cost-effective and sustainable manner. Aquatic ecology can play an important role to achieve the scale-up of algal crop production by informing the supply rates of nutrients to the cultivation systems, and by helping to create adaptive production systems that are resilient to

  8. Further Characterization of CELSS Wastes: A Review of Solid Wastes Present to Support Potential Secondary Biomass Production

    NASA Technical Reports Server (NTRS)

    Muller, Matthew S.

    1996-01-01

    Controlled ecological life support systems (CELSS) may one day play an essential role in extraterrestrial colonies. Key to the success of any CELSS will be the system's ability to approach a self-supporting status through recovery and reuse of basic resources. Primary CELSS solid wastes with potential to support secondary biomass production will be inedible plant biomass and metabolic human wastes. Solid waste production is summarized and reported as 765 g N per day per person, including 300 g C and 37 g N per day per person. One Resource Recovery configuration using the bioprocessing of solid wastes into a Tilapia feed stream is examined. Based on estimated conversion efficiencies, 12 g of protein per day per person is produced as a nutrition supplement. The unique tissue composition of crops produced at the Kennedy Space Center CELSS Program highlights the need to evaluate Resource Recovery components with data generated in the CELSS environment.

  9. Predicting Consumer Biomass, Size-Structure, Production, Catch Potential, Responses to Fishing and Associated Uncertainties in the World's Marine Ecosystems.

    PubMed

    Jennings, Simon; Collingridge, Kate

    2015-01-01

    Existing estimates of fish and consumer biomass in the world's oceans are disparate. This creates uncertainty about the roles of fish and other consumers in biogeochemical cycles and ecosystem processes, the extent of human and environmental impacts and fishery potential. We develop and use a size-based macroecological model to assess the effects of parameter uncertainty on predicted consumer biomass, production and distribution. Resulting uncertainty is large (e.g. median global biomass 4.9 billion tonnes for consumers weighing 1 g to 1000 kg; 50% uncertainty intervals of 2 to 10.4 billion tonnes; 90% uncertainty intervals of 0.3 to 26.1 billion tonnes) and driven primarily by uncertainty in trophic transfer efficiency and its relationship with predator-prey body mass ratios. Even the upper uncertainty intervals for global predictions of consumer biomass demonstrate the remarkable scarcity of marine consumers, with less than one part in 30 million by volume of the global oceans comprising tissue of macroscopic animals. Thus the apparently high densities of marine life seen in surface and coastal waters and frequently visited abundance hotspots will likely give many in society a false impression of the abundance of marine animals. Unexploited baseline biomass predictions from the simple macroecological model were used to calibrate a more complex size- and trait-based model to estimate fisheries yield and impacts. Yields are highly dependent on baseline biomass and fisheries selectivity. Predicted global sustainable fisheries yield increases ≈4 fold when smaller individuals (< 20 cm from species of maximum mass < 1 kg) are targeted in all oceans, but the predicted yields would rarely be accessible in practice and this fishing strategy leads to the collapse of larger species if fishing mortality rates on different size classes cannot be decoupled. Our analyses show that models with minimal parameter demands that are based on a few established ecological principles

  10. Biotechnology in biomass crop production

    SciTech Connect

    Harry, D.E.; Sederoff, R.R. . Dept. of Forestry; North Carolina State Univ., Raleigh, NC . Dept. of Forestry)

    1989-12-01

    There is great potential for the application of plant genetic engineering to production of biomass crops. Two factors largely determine the feasibility of crop improvement using genetic engineering: (1) the status of technology for the transfer and expression of foreign genetic material in plants, and (2) the level of understanding about genetic factors involved in the process or trait to be manipulated. Although information and technology for exploiting the potential of genetic engineering is in an early developmental phase, new research initiatives can now be taken to make significant advances. In this report we evaluate the nature and status of information and technology relating to specific problems of interest for production of biomass crops. In our discussions, we emphasize woody crops because our expertise is stronger in this area, but we have included information on herbaceous crops as appropriate. Plant genetic engineering has the potential to improve plantation or stand establishment, biomass production, and structural or chemical properties for efficient biomass utilization by either direct combustion or by conversion. Such research programs must be highly interdisciplinary, involving new technologies in laboratory research, in addition to traditional field testing. 119 refs.

  11. Wood biomass: The potential of willow

    SciTech Connect

    White, E.H.; Abrahamson, L.P. . Coll. of Environmental Science and Forestry)

    1991-10-01

    Experiments were established in central New York State in spring, 1987, to evaluate the potential of Salix for wood biomass production using ultrashort-rotation intensive-culture techniques. Five selected willow clones and one hybrid poplar clone planted at 1 {times} 1 foot spacing were tested for biomass production with annual coppicing. This report presents results of this research as of December 31, 1990. (VC)

  12. Biomass in Serbia - potential of beech forests

    NASA Astrophysics Data System (ADS)

    Brasanac-Bosanac, Lj.; Cirkovic-Mitrovic, T.; Popovic, V.; Jokanovic, D.

    2012-04-01

    As for the renewable sources for energy production, biomass from forests and wood processing industry comes to the second place. The woody biomass accounts for 1.0 Mtoe, that is equivalent with 1.0 Mtoe of oil. Due to current evaluations, the greatest part of woody biomass would be used for briquettes and pallets production. As the biomass from forests is increasingly becoming the interest of national and international market, a detailed research on overall potential of woody supply from Serbian forests is required. Beech forests account for 29.4 % of forest cover of Serbia. They also have the greatest standing volume (42.4 % of the overall standing volume) and the greatest mean annual increment (32.3 %)(Bankovic,et.al.2009). Herewith, the aim of this poster is to determine the long-term biomass production of these forests.For this purpose a management unit called Lomnicka reka has been chosen. As these beech forests have similar structural development, this location is considered representative for whole Serbia. DBH of all trees were measured with clipper and the accuracy of 0.01 mm, and the heights with a Vertex 3 device (with accuracy of 0.1 m). All measurements were performed on the fields each 500 m2 (square meters). The overall quantity of root biomass was calculated using the allometric equations. The poster shows estimated biomass stocks of beech forests located in Rasina area. Dates are evaluated using non-linear regression (Wutzler,T.et.al.2008). Biomass potential of Serbian beech forests will enable the evaluation of long-term potential of energy generation from woody biomass in agreement with principles of sustainable forest management. The biomass from such beech forests can represent an important substitution for energy production from fossil fuels (e.g. oil) and herewith decrease the CO2 emissions.

  13. Energy production from biomass (Part 1): Overview of biomass.

    PubMed

    McKendry, Peter

    2002-05-01

    The use of renewable energy sources is becoming increasingly necessary, if we are to achieve the changes required to address the impacts of global warming. Biomass is the most common form of renewable energy, widely used in the third world but until recently, less so in the Western world. Latterly much attention has been focused on identifying suitable biomass species, which can provide high-energy outputs, to replace conventional fossil fuel energy sources. The type of biomass required is largely determined by the energy conversion process and the form in which the energy is required. In the first of three papers, the background to biomass production (in a European climate) and plant properties is examined. In the second paper, energy conversion technologies are reviewed, with emphasis on the production of a gaseous fuel to supplement the gas derived from the landfilling of organic wastes (landfill gas) and used in gas engines to generate electricity. The potential of a restored landfill site to act as a biomass source, providing fuel to supplement landfill gas-fuelled power stations, is examined, together with a comparison of the economics of power production from purpose-grown biomass versus waste-biomass. The third paper considers particular gasification technologies and their potential for biomass gasification. PMID:12058829

  14. Estimation of potential biomass resource and biogas production from aquatic plants in Argentina

    SciTech Connect

    Fitzsimons, R.E.; Laurino, C.N.; Vallejos, R.H.

    1982-01-01

    The Argentine government's Agua y Energia Electrica is planning to construct a hydroelectric power-generation facility on the middle Parana River, which is already heavily infested with aquatic weeds such as water hyacinth. These species will probably proliferate in the lakes that will be formed by the power project and perhaps seriously interfere with the facility. As a solution to this problem, Argentine biochemists propose mechanical harvesting and anaerobic fermentation of the aquatic plants to produce biogas and fertilizer. According to an evaluation of this potential resource, gross methane production could reach 37-153 billion CF (1.0-4.1 billion m/sup 3/)/yr, and the digested residue could provide 60,500-244,000 tons (54,900-221,400 metric tons)/yr of nitrogen, which represents 2-8 times Argentina's current nitrogen fertilizer demand.

  15. Freshwater aquatic plant biomass production in Florida

    SciTech Connect

    Reddy, K.R.; Sutton, D.L.; Bowes, G.

    1983-01-01

    About 8% (1.2 million ha) of the total surface area of Florida is occupied by freshwater. Many of these water bodies are eutrophic. Nutrients present in these water bodies can be potentially used to culture aquatic plants as a possible feedstock for methane production. This paper summarizes the results of known research findings on biomass production potential of freshwater aquatic plants in Florida and identifies key research needs to improve the quality and quantity of biomass yields. Among floating aquatic plants, biomass yield potential was in the order of water-hyacinth > water lettuce > pennywort > salvinia > duckweed > azolla. Pennywort, duckweed, and azolla appear to perform well during the cooler months compared to other aquatic plants. Among emergent plants, biomass yield potential was in the order of southern wild rice > cattails > soft rush > bulrush. Cultural techniques, nutrient management, and environmental factors influencing the biomass yields were discussed. 68 references.

  16. Potential Harvestable Corn Cob Biomass in Several Production Systems in the Western Corn Belt

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The proposed use of corn residues for biofuel production has increased interest in how much and what components of residue should/can be removed. One component of corn residue that is already being handled (corn cobs) might be an easily harvestable product that could be used for biofuel production. ...

  17. The potential impacts of biomass feedstock production on water resource availability

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biofuels are a major area of interest and technology development globally. Whereas bioenergy crop production is highly dependent on water, bioenergy development requires effective allocation and management of water. The objectives of this investigation were to assess the bioenergy production relativ...

  18. Predicting potential biomass and N production of Sunn hemp when grown as a cover crop in the south

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sunn hemp (Crotalaria juncea) has the potential to become an important green manure and cover crop in the South because it produces significant quantities of biomass and nitrogen in a short period of time. For successful incorporation into conservation management systems producers need a tool to pre...

  19. Chlorella mirabilis as a Potential Species for Biomass Production in Low-Temperature Environment

    PubMed Central

    Shukla, S. P.; Kvíderová, J.; Tříska, J.; Elster, J.

    2013-01-01

    Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabilis under different nitrogen and carbon sources followed by analyses of fatty acid contents. The micro-scale mass cultivation was performed in stable (in-door) and variable (out-door) conditions during winter and/or early spring in the Czech Republic. In the in-door cultivation, the treatments for nitrogen and carbon sources determination included pure Z medium (control, Z), Z medium + 5% glycerol (ZG), Z medium + 5% glycerol + 50 μM KNO3 (ZGN), Z medium + 5% glycerol + 200 μM NH4Cl (ZGA), Z medium + 5% glycerol + 1 mM Na2CO3 (ZNC), Z medium + 5% glycerol + 1 mM Na2CO3 + 200 μM NH4Cl (ZGCA) and Z medium + 5% glycerol + 1 mM Na2CO3 + 50 μM KNO3 (ZGCN) and were performed at 15°C with an irradiance of 75 μmol m−2 s−1. During the out-door experiments, the night-day temperature ranged from −6.6 to 17.5°C (daily average 3.1 ± 5.3°C) and irradiance ranged from 0 to 2,300 μmol m−2 s−1 (daily average 1,500 ± 1,090 μmol m−2 s−1). Only the Z, ZG, ZGN, and ZGC treatments were used in the out-door cultivation. In the in-door mass cultivation, all nitrogen and carbon sources additions increased the growth rate with the exception of ZGA. When individual sources were considered, only the effect of 5% glycerol addition was significant. On the other hand, the growth rate decreased in the ZG and ZGN treatments in the out-door experiment, probably due to carbon limitation. Fatty acid composition showed increased production of linoleic acid in the glycerol treatments. The studied strain of C. mirabilis is proposed to be a promising source of linoleic acid in low

  20. Biomass production and nutrient removal potential of water hyacinth cultured in sewage effluent

    SciTech Connect

    Reddy, K.R.; Hueston, F.M.; McKinn, T.

    1985-05-01

    Growth and nutrient uptake of water hyacinth (Eichhornia crassipes (Mart Solms)) cultured in sewage effluent were measured over a period of one year in a prototype wastewater treatment system which has been in operation at Walt Disney World near Orlando, Florida. Annual productivity of water hyacinth cultured in primary sewage effluent (Channel II) was found to be in the range of 5 to 27 g dry wt/m/sup 2/ day (23.6 dry tons/acre yr). Average growth rate during the months of May through October 1982 for hyacinth cultured in Channel II (primary sewage effluent) and Channel I (treated primary sewage effluent leaving Channel II) was about 16 g dry wt/m/sup 2/ (27 dry tons/acre yr), compared to the growth rate of 13 g dry wt/m/sup 2/ (22 dry tons/acre yr) for hyacinths cultured in secondary sewage effluent. Plants cultured in secondary sewage effluent generally had longer roots than the plants cultured in primary sewage effluent. A significant relationship was observed between the growth rate of hyacinth and the solar radiation. N and P concentration of the plant tissue were higher in the hyacinths cultured during winter months compared to the plants grown in summer months. Average N and P concentration of the plants cultured im primary sewage effluent were found to be 3.7% N and 0.94% P, respectively, while the plants cultured in secondary sewage effluent had a total N and P content of 2.8% N and 0.79% P. Nutrient ratios of the major plant nurtrients were found to be approximately the same as the nutrient ratios in the sewage effluent. Annual N and P uptake rates of hyacinth cultured in sewage effluent were found to be in the range of 1176 to 1193 kg N/ha yr and 321 to 387 kg P/ha yr, respectively.

  1. Biomass Production and Soil Carbon

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There is expanding interest in harvesting crop biomass for energy. Crop biomass such as corn stover, wheat straw, soybean straw or other crop straws can be used as feedstock to support several bioenergy platforms (cellulosic ethanol, gasification or pyrolysis). There are potential benefits for using...

  2. Biomass production and nutrient removal potential of water hyacinth cultured in sewage effluent

    SciTech Connect

    Reddy, K.R.; Hueston, F.M.; McKim, T.

    1985-05-01

    Growth and nutrient uptake of water hyacinth (Eichhornia crassipes (Mart) Solms) cultured in sewage effluent were measured over a period of 1 year in a prototype wastewater treatment system which has been in operation at Walt Disney World near Orlando, Florida. Annual productivity of water hyacinth cultured in primary sewage effluent (Channel II) was found to be in the range of 5 to 27 g dry wt m/sup -2/ day/sup -1/ (23.6 dry tons acre/sup -1/ yr/sup -1/). Average growth rate during the months of May through October 1982 for hyacinth cultured in Channel II (primary sewage effluent) and Channel I (treated primary sewage effluent leaving Channel II) was about 16 g dry wt m/sup -2/ day/sup -1/ (27 dry tons acre/sup -1/ yr/sup -1/), compared to the growth rate of 13 g dry wt m/sup -2/ day/sup -1/ (22 dry tons acre/sup -1/ yr/sup -1/) for hyacinths cultured in secondary sewage effluent. Plants cultured in secondary sewage effluent generally had longer roots than the plants cultured in primary sewage effluent. A significant relationship was observed between the growth rate of hyacinth and the solar radiation. Nitrogen and P concentration of the plant tissue were higher in the hyacinths cultured during winter months compared to the plants grown in summer months. Average N and P concentration of the plants cultured in primary sewage effluent were found to be 3.7 percent N and 0.94 percent P, respectively, while the plants cultured in secondary sewage effluent had a total N and P content of 2.8 percent N and 0.79 percent P. Nutrient ratios of the major plant nutrients were found to be approximately the same as the nutrient ratios in the sewage effluent. Annual N and P uptake rates of hyacinth cultured in sewage effluent were found to be in the range of 1176 to 1193 kg N ha/sup -1/ yr/sup -1/ and 321 to 387 kg P ha/sup -1/ yr/sup -1/, respectively.

  3. Analytical pyrolysis of synthetic chars derived from biomass with potential agronomic application (biochar). Relationships with impacts on microbial carbon dioxide production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A set of 20 biochar samples produced from the pyrolysis of different biomass feedstocks with potential applications as soil amendments were investigated by pyrolysis coupled to gas chromatography-mass spectrometry (Py-GC-MS). The yields of 38 pyrolysis products representative of charred (e.g., benze...

  4. Lignocellulose biomass production potential from poplar short rotation plantations on marginal land in Germany and their impact on soil organic carbon stocks

    NASA Astrophysics Data System (ADS)

    Haas, Edwin; Klatt, Steffen; Kiese, Ralf; Werner, Christian; Butterbach-Bahl, Klaus

    2013-04-01

    In this study we assess the potential of lignocellulose biomass production by short rotation plantations in Germany. To avoid conflicts with agricultural food production only marginal agricultural land was accounted, which is usually of low quality and productivity. The process-oriented biogeochemical model LandscapeDNDC was used in conjunction with the forest-growth model PSIM to simulate the yield of poplar grown in short-rotation plantations throw-out Germany. The model was validated on five sites with different climatic conditions in Central Europe. The study aims to assess the effects of biomass short rotation plantations on the soil organic carbon stocks in Germany by comparing the cultivation of the bioenergy crops against the former arable land use (taken from the NitroEurope database). Using regional model input, with climatic drivers and soil properties being the most important, the biomass production potential of poplar plantations was simulated. To limit short-term climatic effects on the simulation outcome, we simulated biomass yields from short-rotation (6 year) Populus plantations for three time slices (1990-1995, 2000-2005, and 2010-2015) with climate data taken from the NitroEurope database and provided the simulated yield averages and standard deviations of these runs as well as the changes in soil organic carbon stocks compared to the former land use due to the land use change. Considering 10% of the arable land with the lowest productivity, the model results in a biomass production of approximately 6.78 t DM / ha / year which is approx. 10.1 kilo t DM / year while the 10% of the arable land with the highest productivity result in 8.11 t DM / ha / year which is approx. 12.6 kilo t DM / year (averages over 3 simulation time slices). We will present results of transient simulations of several rotations with various rotation lengths for biomass yields and changes in soil organic carbon stocks.

  5. Pilot-scale comparison of four duckweed strains from different genera for potential application in nutrient recovery from wastewater and valuable biomass production.

    PubMed

    Zhao, Y; Fang, Y; Jin, Y; Huang, J; Bao, S; Fu, T; He, Z; Wang, F; Wang, M; Zhao, H

    2015-01-01

    The application potential of four duckweed strains from four genera, Wolffia globosa 0222, Lemna japonica 0223, Landoltia punctata 0224 and Spirodela polyrhiza 0225, were compared in four parallel pilot-scale wastewater treatment systems for more than 1 year. The results indicated that each duckweed strain had unique potential advantages. Unlike L. japonica 0223 and La. punctata 0224, which grow throughout the year, S. polyrhiza 0225 and W. globosa 0222 do not survive cold weather. For year round performance, L. japonica 0223 was best not only in dry biomass production (6.10 g·m(-2) ·day(-1) ), but also in crude protein (35.50%), total amino acid (26.83%) and phosphorus (1.38%) content, plus recovery rates of total nitrogen (TN), total phosphorus (TP) and CO2 (0.31, 0.085 and 7.76 g·m(-2) ·day(-1) , respectively) and removal rates of TN and TP (0.66 and 0.089 g·m(-2) ·day(-1) , respectively). This strongly demonstrates that L. japonica 0223 performed best in wastewater treatment and protein biomass production. Under nutrient starvation conditions, La. punctata 0224 had the highest starch content (45.84%), dry biomass production (4.81 g·m(-2) ·day(-1) ) and starch accumulation (2.9 g·m(-2) ·day(-1) ), making it best for starch biomass production. W. globosa 0222 and S. polyrhiza 0225 showed increased flavonoid biomass production, with higher total flavonoid content (5.85% and 4.22%, respectively) and high dominant flavonoids (>60%). This study provides useful information for selecting the appropriate local duckweed strains for further application in wastewater treatment and valuable biomass production. PMID:24942851

  6. Winter crop and residue biomass potential in China

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper estimates the potential biomass production from winter crops and summer crop residues in China. Rye is used to represent winter crop production, and straw from corn, wheat and rice is used to represent residue potential. Biomass totals are intended for use as energy feedstocks and are ass...

  7. Microalgae biomass growth using primary treated wastewater as nutrient source and their potential use for lipids production

    NASA Astrophysics Data System (ADS)

    Frementiti, Anastacia; Aravantinou, Andriana F.; Manariotis, Ioannis D.

    2015-04-01

    The great demand for energy, the rising price of the crude oil and the rapid decrease of the supply of fossil fuels are the main reasons that have increased the interest for the production of fuels from renewable resources. Microalgae are considered to be the most promising new source of biomass and biofuels, since their lipid content in some cases is up to 70%. The microalgal growth and its metabolism processes are essential in wastewater treatment with many economical prospects. The aim of this work was to evaluate the algal production in a laboratory scale open pond. The pond had a working volume of 30 L and was fed with sterilized primary treated wastewater. Chlorococcum sp. was used as a model microalgal. Experiments were conducted under controlled environmental conditions in order to investigate the removal of nutrients, biomass growth, and lipids accumulation in microalgae. Chlorococcum sp. cultures behavior was investigated under batch, fill and draw, and continuous operation mode, at two different radiation intensities (100 and 200 μmol/m2s). The maximum biomass concentration of 630 mg/L was observed with the fill and draw mode. Moreover, the growth rates of microalgal biomass were depended on the influent nutrients concentration. Specifically, the phosphates were the limiting factor for biomass growth in continuous condition; the phosphates removal in this condition, reached a 100%. Chemical demand oxygen (COD) was not removed efficiently by Chlorococcum sp. since it was an autotrophic microalgal with no organic carbon demands for its growth. The lipids content in the dry weight of Chlorococcum sp. ranged from 1 to 9% depending on the concentration of nutrients and the operating conditions.

  8. New market potential: Torrefaction of Woody Biomass

    SciTech Connect

    Jaya Shankar Tumuluru; J. Richard Hess

    2015-07-01

    According to researchers in Idaho National Laboratory’s Bioenergy Program, torrefaction of woody biomass could reduce variability in biomass feedstock and enable development of a commodity-type product for green energy generation and usage.

  9. Biomass Production Potential of a Wastewater Alga Chlorella vulgaris ARC 1 under Elevated Levels of CO2 and Temperature

    PubMed Central

    Chinnasamy, Senthil; Ramakrishnan, Balasubramanian; Bhatnagar, Ashish; Das, Keshav C.

    2009-01-01

    The growth response of Chlorella vulgaris was studied under varying concentrations of carbon dioxide (ranging from 0.036 to 20%) and temperature (30, 40 and 50°C). The highest chlorophyll concentration (11 μg mL–1) and biomass (210 μg mL–1), which were 60 and 20 times more than that of C. vulgaris at ambient CO2 (0.036%), were recorded at 6% CO2 level. At 16% CO2 level, the concentrations of chlorophyll and biomass values were comparable to those at ambient CO2 but further increases in the CO2 level decreased both of them. Results showed that the optimum temperature for biomass production was 30°C under elevated CO2 (6%). Although increases in temperature above 30°C resulted in concomitant decrease in growth response, their adverse effects were significantly subdued at elevated CO2. There were also differential responses of the alga, assessed in terms of NaH14CO3 uptake and carbonic anhydrase activity, to increases in temperature at elevated CO2. The results indicated that Chlorella vulgaris grew better at elevated CO2 level at 30°C, albeit with lesser efficiencies at higher temperatures. PMID:19333419

  10. Photoinduced Biohydrogen Production from Biomass

    PubMed Central

    Amao, Yutaka

    2008-01-01

    Photoinduced biohydrogen production systems, coupling saccharaides biomass such as sucrose, maltose, cellobiose, cellulose, or saccharides mixture hydrolysis by enzymes and glucose dehydrogenase (GDH), and hydrogen production with platinum colloid as a catalyst using the visible light-induced photosensitization of Mg chlorophyll-a (Mg Chl-a) from higher green plant or artificial chlorophyll analog, zinc porphyrin, are introduced. PMID:19325796

  11. Biomass resource potential using energy crops

    SciTech Connect

    Wright, L.L.; Cushman, J.H.; Martin, S.A.

    1993-09-01

    Biomass energy crops can provide a significant and environmentally beneficial source of renewable energy feedstocks for the future. They can revitalize the agricultural sector of the US economy by providing profitable uses for marginal cropland. Energy crops include fast-growing trees, perennial grasses, and annual grasses, all capable of collecting solar energy and storing it as cellulosic compounds for several months to several years. Once solar energy is thus captured, it can be converted by means of currently available technologies to a wide variety of energy products such as electricity, heat, liquid transportation fuels, and gases. Experimental results from field trials have generated optimism that selected and improved energy crops, established on cropland with moderate limitations for crop production, have the potential for producing high yields. Both trees and grasses, under very good growing conditions, have produced average annual yields of 20 to 40 dry Mg ha{sup {minus}1} year{sup {minus}1}. Sorghum has shown especially high yields in the Midwest. Hybrids between sugar cane and its wild relatives, called energy cane, have yielded as much as 50 dry Mg ha{sup {minus}1} year{sup {minus}1} in Florida. These experimental results demonstrate that some species have the genetic potential for very rapid growth rates. New wood energy crop systems developed by the Department of Energy`s Biofuels Feedstock Development Program offer, at a minimum, a 100% increase in biomass production rates over the 2 to 4 Mg ha{sup {minus}1} year{sup {minus}1} of dry leafless woody biomass produced by most natural forest systems. Experimental data indicate that short rotation wood crops established on cropland with moderate limitations are capable of producing biomass yields of 8--20 dry Mg ha{sup {minus}1} year{sup {minus}1} with a present average about 11 dry Mg ha{sup {minus}1} year{sup {minus}1} on typical cropland sites.

  12. Miscanthus as cellulosic biomass for bioethanol production.

    PubMed

    Lee, Wen-Chien; Kuan, Wei-Chih

    2015-06-01

    The members of the genus Miscanthus are potential feedstocks for biofuels because of the promising high yields of biomass per unit of planted area. This review addresses species, cultivation, and lignocellulose composition of Miscanthus, as well as pretreatment and enzyme saccharification of Miscanthus biomass for ethanol fermentation. The average cellulose contents in dried biomass of Miscanthus floridulus, Miscanthus sinensis, Miscanthus sacchariflorus, and Miscanthus × giganteus (M × G) are 37.2, 37.6, 38.9, and 41.1% wt/wt, respectively. A number of pretreatment methods have been applied in order to enhance digestibility of Miscanthus biomass for enzymatic saccharification. Pretreatment of Miscanthus using liquid hot water or alkaline results in a significant release of glucose; while glucose yields can be 90% or higher if a pretreatment like AFEX that combines both chemical and physical processes is used. As ethanol is produced by yeast fermentation of the hydrolysate from enzymatic hydrolysis of residual solids (pulp) after pretreatment, theoretical ethanol yields are 0.211-0.233 g/g-raw biomass if only cellulose is taken into account. Simultaneous saccharification and fermentation of pretreated M × G and M. lutarioriparius results in experimental ethanol yields of 0.13 and 0.15 g/g-raw biomass, respectively. Co-production of value-added products can reduce the overall production cost of bioethanol. PMID:26013948

  13. Microbial biomass production and evaluation

    SciTech Connect

    Al-Shoshan, A.A.

    1986-01-01

    A study of microbial biomass production was conducted to determine the amount that certain microorganisms can produce. The microorganisms, grown in their optimum conditions, were put into media containing 1% (w/v) corn starch. Those that yielded the greatest quantity of biomass were then utilized in corn meal fermentation. Their effect upon the protein nutritional value of the product was also determined. A comparative study was then carried out to determine the amylolytic activity, and biomass produced by single and mixed cultures of microorganisms including Bacillus sterothermophilus, Endomycopsis fibuligera, Saccharomyces diastaticus, and Candida utilis. In bacteria-yeast fermentation, the highest biomass (4.3 mg/ml) was obtained from C. utilis following B. sterothermophilus. In another fermentation process, yeast-yeast, the highest biomass (6.73 mg/ml) was obtained from a mixed culture of E. fibuligera and C. utilis. In both groups of microorganisms, the level of crude protein, crude fiber, percent relative nutritive value (%RNV) and total available amino acids of lysine, methionine and tryptophan increased. The increase made by the mixed cultures of yeast was superior and was the only treatment that showed a significant (P > 0.05) difference from the non-fermented control and from other treatments.

  14. Assessment of the Projected One Billion Ton Biomass for Cellulosic Biofuel Production and Its Potential Implications on Regional Water Quality and Availability

    NASA Astrophysics Data System (ADS)

    Demissie, Y. K.; Yan, E.; Wu, M.

    2011-12-01

    The DOE and USDA joint study, also commonly referred as the "Billion-Ton" study, assessed the cellulosic feedstock resources potential in the U.S. for producing second generation biofuel to replace 30 percent of the country's transportation fuels by year 2030. The available resource is expected to come from changing cropping pattern, increasing crop yield, harvesting agricultural and forest wood residues, and developing energy crops. Such large-scale changes in land use and crop managements are likely to affect the associated water quality and resources at both regional and local scales. To address the water sustainability associated with the projected biomass production in the Upper Mississippi River Basin (UMRB), we have developed a SWAT watershed model that simulate the changes in water quality (nitrogen, phosphorus, and soil erosion) and resources (soil water content, evapotranspiration, and runoff) of the region due to future biomass production scenario estimated by the Billion-Ton study. The scenario is implemented by changing the model inputs and parameters at subbasin and hydrologic response unit levels, as well as by improving the SWAT model to represent spatially varying crop properties. The potential impacts on water quality and water availability were compared with the results obtained from a baseline simulation which represents current watershed conditions and existing level of feedstock production. The basin level results suggested mixed effects on the water quality. The projected large-scale biomass production scenario is expected to decrease loadings of total nitrogen and nitrate in the streams while increase total phosphorus and suspended sediment. Results indicate an increase in the rate of evapotranspiration and a decrease in the soil water content and in surface runoff. discharge to the streams. The impacts at the subbasin or local scale varies spatially and temporally depending on the types of land use change, their locations, and crop

  15. Molecular characterisation and biomass and metabolite production of Lactobacillus reuteri LPB P01–001: A potential probiotic

    PubMed Central

    Pancheniak, Elizete de F. R.; Maziero, Maike T.; Rodriguez-León, José A.; Parada, José L.; Spier, Michele R.; Soccol, Carlos R.

    2012-01-01

    Lactobacillus reuteri LPB P01–001 was isolated from the gastrointestinal tract of wild swine and was characterised by biochemical testing and sequencing of gene 16S rRNA. A simple and low-cost culture medium based on cane sugar (2.5% p/v) and yeast extract (1% p/v) was used in the production of this probiotic. The fermentative conditions were a) pH control at 6.5 and b) no pH control; both were set at 37°C in a 12 L slightly stirred tank bioreactor. Fermentation parameters such as the specific growth rate, productivity and yield of biomass, lactic and acetic acid levels were determined. L. reuteri LPB P01–001 behaves as an aciduric bacteria because it grows better in a low pH medium without pH control. However, the lactic acid production yield was practically half (9.22 g.L-1) of that obtained under a constant pH of 6.5, which reached 30.5 g.L-1 after 28 hours of fermentation. The acetic acid production was also higher under pH-controlled fermentation, reaching 10.09 g.L-1after 28 hours of fermentation. These parameters may raise the interest of those committed to the efficient production of a probiotic agent for swine. PMID:24031812

  16. A feasibility study of agricultural and sewage biomass as biochar, bioenergy and biocomposite feedstock: production, characterization and potential applications.

    PubMed

    Srinivasan, Prakash; Sarmah, Ajit K; Smernik, Ron; Das, Oisik; Farid, Mohammed; Gao, Wei

    2015-04-15

    In this study, we pyrolysed six waste derived biomass: pine sawdust (PSD), paunch grass (PG), broiler litter (BL), sewage sludge (SS), dewatered pond sludge (DWP), and dissolved air-floatation sludge (DAF) into biochar. Biochars were characterized using scanning electron microscopy, energy dispersive X-ray spectrometry, X-ray diffraction, Fourier transform infrared spectroscopy, inductively-coupled plasma mass spectrometry, (13)C-solid-state nuclear magnetic resonance spectroscopy, and X-ray photoelectron spectroscopy to evaluate their feasibility for potential agronomic and environmental applications. Syngas produced during the pyrolysis process was also analyzed to determine the energy values. Results show that PSD biochar has the utmost potential for carbon sequestration and contaminant remediation due to its high surface area, aromaticity and carbon content. Additionally given its low ash content, PSD biochar could also potentially be used as filler in wood plastic biocomposites. Low levels of heavy metals (Cr, Cu, Zn, As, Cd, Hg, and Pb) in all biochars suggest that biochars are also applicable for land application according to the United States Environmental Protection Agency regulation 40 CFR part 503. The composition of syngas evolved during the pyrolysis of feedstocks showed little difference in the calorific values, ranging from 12-16 MJ/dsm with PSD having the maximum calorific value of 16 MJ/dsm. PMID:25644846

  17. Predicting Consumer Biomass, Size-Structure, Production, Catch Potential, Responses to Fishing and Associated Uncertainties in the World’s Marine Ecosystems

    PubMed Central

    Jennings, Simon; Collingridge, Kate

    2015-01-01

    Existing estimates of fish and consumer biomass in the world’s oceans are disparate. This creates uncertainty about the roles of fish and other consumers in biogeochemical cycles and ecosystem processes, the extent of human and environmental impacts and fishery potential. We develop and use a size-based macroecological model to assess the effects of parameter uncertainty on predicted consumer biomass, production and distribution. Resulting uncertainty is large (e.g. median global biomass 4.9 billion tonnes for consumers weighing 1 g to 1000 kg; 50% uncertainty intervals of 2 to 10.4 billion tonnes; 90% uncertainty intervals of 0.3 to 26.1 billion tonnes) and driven primarily by uncertainty in trophic transfer efficiency and its relationship with predator-prey body mass ratios. Even the upper uncertainty intervals for global predictions of consumer biomass demonstrate the remarkable scarcity of marine consumers, with less than one part in 30 million by volume of the global oceans comprising tissue of macroscopic animals. Thus the apparently high densities of marine life seen in surface and coastal waters and frequently visited abundance hotspots will likely give many in society a false impression of the abundance of marine animals. Unexploited baseline biomass predictions from the simple macroecological model were used to calibrate a more complex size- and trait-based model to estimate fisheries yield and impacts. Yields are highly dependent on baseline biomass and fisheries selectivity. Predicted global sustainable fisheries yield increases ≈4 fold when smaller individuals (< 20 cm from species of maximum mass < 1kg) are targeted in all oceans, but the predicted yields would rarely be accessible in practice and this fishing strategy leads to the collapse of larger species if fishing mortality rates on different size classes cannot be decoupled. Our analyses show that models with minimal parameter demands that are based on a few established ecological principles

  18. Biomass energy: the scale of the potential resource.

    PubMed

    Field, Christopher B; Campbell, J Elliott; Lobell, David B

    2008-02-01

    Increased production of biomass for energy has the potential to offset substantial use of fossil fuels, but it also has the potential to threaten conservation areas, pollute water resources and decrease food security. The net effect of biomass energy agriculture on climate could be either cooling or warming, depending on the crop, the technology for converting biomass into useable energy, and the difference in carbon stocks and reflectance of solar radiation between the biomass crop and the pre-existing vegetation. The area with the greatest potential for yielding biomass energy that reduces net warming and avoids competition with food production is land that was previously used for agriculture or pasture but that has been abandoned and not converted to forest or urban areas. At the global scale, potential above-ground plant growth on these abandoned lands has an energy content representing approximately 5% of world primary energy consumption in 2006. The global potential for biomass energy production is large in absolute terms, but it is not enough to replace more than a few percent of current fossil fuel usage. Increasing biomass energy production beyond this level would probably reduce food security and exacerbate forcing of climate change. PMID:18215439

  19. Energy Potential of Biomass from Conservation Grasslands in Minnesota, USA

    PubMed Central

    Jungers, Jacob M.; Fargione, Joseph E.; Sheaffer, Craig C.; Wyse, Donald L.; Lehman, Clarence

    2013-01-01

    Perennial biomass from grasslands managed for conservation of soil and biodiversity can be harvested for bioenergy. Until now, the quantity and quality of harvestable biomass from conservation grasslands in Minnesota, USA, was not known, and the factors that affect bioenergy potential from these systems have not been identified. We measured biomass yield, theoretical ethanol conversion efficiency, and plant tissue nitrogen (N) as metrics of bioenergy potential from mixed-species conservation grasslands harvested with commercial-scale equipment. With three years of data, we used mixed-effects models to determine factors that influence bioenergy potential. Sixty conservation grassland plots, each about 8 ha in size, were distributed among three locations in Minnesota. Harvest treatments were applied annually in autumn as a completely randomized block design. Biomass yield ranged from 0.5 to 5.7 Mg ha−1. May precipitation increased biomass yield while precipitation in all other growing season months showed no affect. Averaged across all locations and years, theoretical ethanol conversion efficiency was 450 l Mg−1 and the concentration of plant N was 7.1 g kg−1, both similar to dedicated herbaceous bioenergy crops such as switchgrass. Biomass yield did not decline in the second or third year of harvest. Across years, biomass yields fluctuated 23% around the average. Surprisingly, forb cover was a better predictor of biomass yield than warm-season grass with a positive correlation with biomass yield in the south and a negative correlation at other locations. Variation in land ethanol yield was almost exclusively due to variation in biomass yield rather than biomass quality; therefore, efforts to increase biomass yield might be more economical than altering biomass composition when managing conservation grasslands for ethanol production. Our measurements of bioenergy potential, and the factors that control it, can serve as parameters for assessing the economic

  20. Sunflower as a potential biomass crop

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biomass resources are essential for bioenergy production. There are two major criteria for determining whether a crop is suitable for energy use. The first is the high dry matter yield per land unit and the second is the net gain in energy amount (the amount of energy produced from the biomass shoul...

  1. Strategies for optimizing algal biology for enhanced biomass production

    SciTech Connect

    Barry, Amanda N.; Starkenburg, Shawn R.; Sayre, Richard T.

    2015-02-02

    One of the most environmentally sustainable ways to produce high-energy density (oils) feed stocks for the production of liquid transportation fuels is from biomass. Photosynthetic carbon capture combined with biomass combustion (point source) and subsequent carbon capture and sequestration has also been proposed in the intergovernmental panel on climate change report as one of the most effective and economical strategies to remediate atmospheric greenhouse gases. To maximize photosynthetic carbon capture efficiency and energy-return-on-investment, we must develop biomass production systems that achieve the greatest yields with the lowest inputs. Numerous studies have demonstrated that microalgae have among the greatest potentials for biomass production. This is in part due to the fact that all alga cells are photoautotrophic, they have active carbon concentrating mechanisms to increase photosynthetic productivity, and all the biomass is harvestable unlike plants. All photosynthetic organisms, however, convert only a fraction of the solar energy they capture into chemical energy (reduced carbon or biomass). To increase aerial carbon capture rates and biomass productivity, it will be necessary to identify the most robust algal strains and increase their biomass production efficiency often by genetic manipulation. We review recent large-scale efforts to identify the best biomass producing strains and metabolic engineering strategies to improve aerial productivity. In addition, these strategies include optimization of photosynthetic light-harvesting antenna size to increase energy capture and conversion efficiency and the potential development of advanced molecular breeding techniques. To date, these strategies have resulted in up to twofold increases in biomass productivity.

  2. Biotechnological production of methanol from waste biomass

    SciTech Connect

    Kozak, R.; Morris, D.

    1995-12-01

    The production of methanol (CH{sub 3}OH) from waste biomass is possible through the use of genetically modified bacteria. The biomass to methanol conversion process makes use of a naturally occurring, direct aerobic enzymatic system referred to as oxidative demethylation. Methoxy groups are stripped off of lignin and lignin like plant substances (approximately fifty percent of all plant biomass) and hydrolyzed to form methanol. Since the biotech process is stoichiometric, potentially every methoxy group in the lignin feedstock can be converted to methanol fuel. Approximately 30-35% of lignin is a methoxy compound that can be converted. Biotechnological conversion could produce up to 100 gallons/ton or 20 billion gallons a year of methanol from waste biomass. Current work has focused on the genetic modification of the enzymatic conversion process to reach commercial production. The goals of this research are; increase product yields, implement an operon {open_quotes}switch{close_quotes} mechanism to exploit multiple feedstocks, and produce environmentally safe by-products. Progress on these topics will be reported.

  3. BIOMASS BURNING AND THE PRODUCTION OF METHANE

    EPA Science Inventory

    Biomass burning and its environmental implications have also become important research elements of the International Geosphere-Bio sphere Program and the International Global Atmospheric Chemistry Project. he production of atmospheric methane (CH4) by biomass burning will be asse...

  4. The economics of biomass production in the United States

    SciTech Connect

    Graham, R.L.; Walsh, M.E.; Lichtenberg, E.; Roningen, V.O.; Shapouri, H.

    1995-12-31

    Biomass crops (e.g. poplar, willow, switchgrass) could become important feedstocks for power, liquid fuel, and chemical production. This paper presents estimates of the potential production of biomass in the US under a range of assumptions. Estimates of potential biomass crop yields and production costs from the Department of Energy`s (DOE) Oak Ridge National Laboratories (ORNL) are combined with measures of land rents from USDA`s Conservation Reserve Program (CRP), to estimate a competitive supply of biomass wood and grass crops. Estimates are made for one potential biomass use--electric power production--where future costs of electricity production from competing fossil fuels set the demand price. The paper outlines the methodology used and limitations of the analysis.

  5. Woody biomass production in waste recycling systems

    SciTech Connect

    Rockwood, D.L.; Snyder, G.H.; Sprinkle, R.R.

    1994-12-31

    Combining woody biomass production with waste recycling offers many mutual advantages, including increased tree growth and nutrient and water reclamation. Three biomass/recycling studies collectively involving Eucalyptus amplifolia, E. camaldulensis, and E. grandis, rapidly growing species potentially tolerant of high water and nutrient levels, are (1) evaluating general potential for water/nutrient recycling systems to enhance woody biomass production and to recycle water and nutrients, (2) documenting Eucalyptus growth, water use, and nutrient uptake patterns, and (3) identifying Eucalyptus superior for water and nutrient uptake in central and southern Florida. In a 1992-93 study assessing the three Eucalyptus species planted on the outside berms of sewage effluent holding ponds, position on the berms (top to bottom) and genotypes influenced tree size. The potential of the trees to reduce effluent levels in the ponds was assessed. In a stormwater holding pond planted in 1993, these Eucalyptus genotypes varied significantly for tree size but not for survival. E. camaldulensis appears generally superior when flooded with industrial stormwater. Potential sizes of ponds needed for different stormwater applications were estimated. Prolonged flooding of 4- and 5-year-old E. camaldulensis with agricultural irrigation runoff has had no observable effects on tree growth or survival. Younger E. camaldulensis, E. amplifolia, and E. grandis were assessed for water use and nutrient uptake during a Summer 1994 flooding.

  6. Evaluation of the biomass potential for the production of lignocellulosic bioethanol from various agricultural residues in Austria and Worldwide

    NASA Astrophysics Data System (ADS)

    Kahr, Heike; Steindl, Daniel; Wimberger, Julia; Schürz, Daniel; Jäger, Alexander

    2013-04-01

    Due to the fact that the resources of fossil fuels are steadily decreasing, researchers have been trying to find alternatives over the past few years. As bioethanol of the first generation is based on potential food, its production has become an increasingly controversial topic. Therefore the focus of research currently is on the production of bioethanol of the second generation, which is made from cellulosic and lignocellulosic materials. However, for the production of bioethanol of the second generation the fibres have to be pre-treated. In this work the mass balances of various agricultural residues available in Austria were generated and examined in lab scale experiments for their bioethanol potential. The residues were pretreatment by means of state of the art technology (steam explosion), enzymatically hydrolysed and fermented with yeast to produce ethanol. Special attention was paid the mass balance of the overall process. Due to the pretreatment the proportion of cellulose increases with the duration of the pre-treatment, whereby the amount of hemicellulose decreases greatly. However, the total losses were increasing with the duration of the pre-treatment, and the losses largely consist of hemicellulose. The ethanol yield varied depending on the cellulose content of the substrates. So rye straw 200 °C 20 min reaches an ethanol yield of 169 kg/t, by far the largest yield. As result on the basis of the annual straw yield in Austria, approximately 210 000 t of bioethanol (266 million litres) could be produced from the straw of wheat (Triticum vulgare), rye (Secale cereale), oat (Avena sativa) and corn (Zea mays) as well as elephant grass (Miscanthus sinensis) using appropriate pre-treatment. So the greenhouse gas emissions produced by burning fossil fuels could be reduced significantly. About 1.8 million tons of motor gasoline are consumed in Austria every year. The needed quantity for a transition to E10 biofuels could thus be easily provided by bioethanol

  7. Fermentable sugar production from lignocellulosic biomass

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fermentable sugar production from lignocellulosic biomass has become an important research focus in the production of renewable biofuels and other bio-products. It means conversion of the carbohydrates contained in the biomass, including cellulose, hemicellose, and/or pectin into their component sug...

  8. Bioconversion of waste biomass to useful products

    DOEpatents

    Grady, James L.; Chen, Guang Jiong

    1998-01-01

    A process is provided for converting waste biomass to useful products by gasifying the biomass to produce synthesis gas and converting the synthesis gas substrate to one or more useful products. The present invention is directed to the conversion of biomass wastes including municipal solid waste, sewage sludge, plastic, tires, agricultural residues and the like, as well as coal, to useful products such as hydrogen, ethanol and acetic acid. The overall process includes the steps of gasifying the waste biomass to produce raw synthesis gas, cooling the synthesis gas, converting the synthesis gas to the desired product or products using anaerobic bioconversion, and then recovering the product or products. In accordance with a particular embodiment of the present invention, waste biomass is converted to synthesis gas containing carbon monoxide and, then, the carbon monoxide is converted to hydrogen by an anaerobic microorganism ERIH2, bacillus smithii ATCC No. 55404.

  9. Bioconversion of waste biomass to useful products

    DOEpatents

    Grady, J.L.; Chen, G.J.

    1998-10-13

    A process is provided for converting waste biomass to useful products by gasifying the biomass to produce synthesis gas and converting the synthesis gas substrate to one or more useful products. The present invention is directed to the conversion of biomass wastes including municipal solid waste, sewage sludge, plastic, tires, agricultural residues and the like, as well as coal, to useful products such as hydrogen, ethanol and acetic acid. The overall process includes the steps of gasifying the waste biomass to produce raw synthesis gas, cooling the synthesis gas, converting the synthesis gas to the desired product or products using anaerobic bioconversion, and then recovering the product or products. In accordance with a particular embodiment of the present invention, waste biomass is converted to synthesis gas containing carbon monoxide and, then, the carbon monoxide is converted to hydrogen by an anaerobic microorganism ERIH2, Bacillus smithii ATCC No. 55404. 82 figs.

  10. Engineering verification of the biomass production chamber

    NASA Technical Reports Server (NTRS)

    Prince, R. P.; Knott, W. M., III; Sager, J. C.; Jones, J. D.

    1992-01-01

    The requirements for life support systems, both biological and physical-chemical, for long-term human attended space missions are under serious study throughout NASA. The KSC 'breadboard' project has focused on biomass production using higher plants for atmospheric regeneration and food production in a special biomass production chamber. This chamber is designed to provide information on food crop growth rate, contaminants in the chamber that alter plant growth requirements for atmospheric regeneration, carbon dioxide consumption, oxygen production, and water utilization. The shape and size, mass, and energy requirements in relation to the overall integrity of the biomass production chamber are under constant study.

  11. Strategies for optimizing algal biology for enhanced biomass production

    DOE PAGESBeta

    Barry, Amanda N.; Starkenburg, Shawn R.; Sayre, Richard T.

    2015-02-02

    One of the most environmentally sustainable ways to produce high-energy density (oils) feed stocks for the production of liquid transportation fuels is from biomass. Photosynthetic carbon capture combined with biomass combustion (point source) and subsequent carbon capture and sequestration has also been proposed in the intergovernmental panel on climate change report as one of the most effective and economical strategies to remediate atmospheric greenhouse gases. To maximize photosynthetic carbon capture efficiency and energy-return-on-investment, we must develop biomass production systems that achieve the greatest yields with the lowest inputs. Numerous studies have demonstrated that microalgae have among the greatest potentials formore » biomass production. This is in part due to the fact that all alga cells are photoautotrophic, they have active carbon concentrating mechanisms to increase photosynthetic productivity, and all the biomass is harvestable unlike plants. All photosynthetic organisms, however, convert only a fraction of the solar energy they capture into chemical energy (reduced carbon or biomass). To increase aerial carbon capture rates and biomass productivity, it will be necessary to identify the most robust algal strains and increase their biomass production efficiency often by genetic manipulation. We review recent large-scale efforts to identify the best biomass producing strains and metabolic engineering strategies to improve aerial productivity. In addition, these strategies include optimization of photosynthetic light-harvesting antenna size to increase energy capture and conversion efficiency and the potential development of advanced molecular breeding techniques. To date, these strategies have resulted in up to twofold increases in biomass productivity.« less

  12. The secretion of the bacterial phytase PHY-US417 by Arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co-growth.

    PubMed

    Belgaroui, Nibras; Berthomieu, Pierre; Rouached, Hatem; Hanin, Moez

    2016-09-01

    Phytic acid (PA) is a major source of inorganic phosphate (Pi) in the soil; however, the plant lacks the capacity to utilize it for Pi nutrition and growth. Microbial phytases constitute a group of enzymes that are able to remobilize Pi from PA. Thus, the use of these phytases to increase the capacity of higher plants to remobilize Pi from PA is of agronomical interest. In the current study, we generate transgenic Arabidopsis lines (ePHY) overexpressing an extracellular form of the phytase PHY-US417 of Bacillus subtilis, which are characterized by high levels of secreted phytase activity. In the presence of PA as sole source of Pi, while the wild-type plants show hallmark of Pi deficiency phenotypes, including the induction of the expression of Pi starvation-induced genes (PSI, e.g. PHT1;4) and the inhibition of growth capacity, the ePHY overexpressing lines show a higher biomass production and no PSI induction. Interestingly, when co-cultured with ePHY overexpressors, wild-type Arabidopsis plants (or tobacco) show repression of the PSI genes, improvement of Pi content and increases in biomass production. In line with these results, mutants in the high-affinity Pi transporters, namely pht1;1 and pht1;1-1;4, both fail to accumulate Pi and to grow when co-cultured with ePHY overexpressors. Taken together, these data demonstrate the potential of secreted phytases in improving the Pi content and enhancing growth of not only the transgenic lines but also the neighbouring plants. PMID:26914451

  13. Biomass production from inland brines

    SciTech Connect

    Reach, C.D. Jr.

    1985-01-01

    The feasibility of utilizing inland saline waters to produce biomass through the application of marine aquaculture was investigated. From available data, the diatom Phaeodactylum tricornutum and the crustacea Artemia salina were selected as the experimental marine organisms. The proposed diatom served to establish primary productivity and concurrently provide a food source for the herbivorus crustacea. The objective of the first phase research was to investigate the ability of P. tricornutum and A. salina to survive in the inland saline environment. Clarified activated sludge and anaerobic digester effluents were evaluated as nutrient sources for the diatom cultures. Experimental results indicated that diatom and crustacea growth in the inland brine was equivalent to control cultures utilizing seawater. Wastewater effluents were successful as nutrient sources for the diatom cultures. Bioassay experiments conducted with petroleum related brines yielded mixed results respect to the survival and growth of the P. tricornutum and A. salina organisms. A second series of experiments involved cholornaphthalene, chlorophenanthene, and chlorophenanthrene, and chloroanthracene as the experimental hydrocarbons. Results of the diatom studies show chloroanthracene to induce toxic effects at a concentration of 500 ug/L. Artemia studies showed no acutely toxic effects relative to the test hydrocarbons at 50 and 100 ug/L.

  14. Polyhydroxyalkanoates production from waste biomass

    NASA Astrophysics Data System (ADS)

    Nor Aslan, A. K. H.; Mohd Ali, M. D.; Morad, N. A.; Tamunaidu, P.

    2016-06-01

    Polyhydroxyalkanoates (PHAs) is a group of biopolymers that are extensively researched for such purpose due to the biocompatibility with mammal tissue and similar properties with conventional plastic. However, commercialization of PHA is impended by its high total production cost, which half of it are from the cost of pure carbon source feedstock. Thus, cheap and sustainable feedstocks are preferred where waste materials from various industries are looked into. This paper will highlight recent studies done on PHA production by utilizing crop and agro waste material and review its potential as alternative feedstock.

  15. Butanol production from renewable biomass by clostridia.

    PubMed

    Jang, Yu-Sin; Malaviya, Alok; Cho, Changhee; Lee, Joungmin; Lee, Sang Yup

    2012-11-01

    Global energy crisis and limited supply of petroleum fuels have rekindled the worldwide focus towards development of a sustainable technology for alternative fuel production. Utilization of abundant renewable biomass offers an excellent opportunity for the development of an economical biofuel production process at a scale sufficiently large to have an impact on sustainability and security objectives. Additionally, several environmental benefits have also been linked with the utilization of renewable biomass. Butanol is considered to be superior to ethanol due to its higher energy content and less hygroscopy. This has led to an increased research interest in butanol production from renewable biomass in recent years. In this paper, we review the various aspects of utilizing renewable biomass for clostridial butanol production. Focus is given on various alternative substrates that have been used for butanol production and on fermentation strategies recently reported to improve butanol production. PMID:22939593

  16. PRODUCTION OF XYLITOL FROM AGRICULTURAL HEMICELLULOSIC BIOMASS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The production of value-added co-products from agricultural biomass is an important economic driver for the success of a biorefinery approach to the production of ethanol and other fuels. During most ethanol production methods, significant amounts of hemicellulose by-products are produced which are...

  17. Potential for biomass electricity in four Asian countries

    SciTech Connect

    Kinoshita, C.M.; Turn, S.Q.; Tantlinger, J.; Kaya, M.

    1997-12-31

    Of all forms of renewable energy, biomass offers the best near-term opportunity for supplying a significant portion of the world`s need for electric power. Biomass is especially competitive when fuel supply costs are partially defrayed as production activities associated with the processing of another product, e.g., sugar, rice, or vegetable oil. Not only do such processing situations provide cost savings, they also generate very large supplies of fuel and therefore can contribute significantly to the local energy mix. Access to ample supplies of competitively-priced biomass feedstocks is only one of several factors needed to encourage the use of biomass for power generation; equally important is a healthy market for electricity, i.e., need for large blocks of additional power and sufficient strength in the economy to attract investment in new capacity. Worldwide, the Asia-Pacific region is projected to have the greatest need for new generating capacity in the next decade and shows the highest rate of economic growth, making it an attractive market for biomass power. Also critical to the expansion of bioenergy is the adoption of positive, stable policies on energy production, distribution, and sale, that encourage the generation and use of electricity from biomass. The aforementioned three factors--adequate biomass supplies, increasing demand for electricity, and supportive policies--are examined for four Asian countries, the Philippines, Thailand, Malaysia, and Indonesia. Information presented for each of the four countries include the types and amounts of bioresidues and their associated electric power generation potential; present and future supplies and demand for electricity; and existing or planned government and utility policies that could impact the generation and use of biomass power.

  18. Flotation of algae for water reuse and biomass production: role of zeta potential and surfactant to separate algal particles.

    PubMed

    Kwak, Dong-Heui; Kim, Mi-Sug

    2015-01-01

    The effect of chemical coagulation and biological auto-flocculation relative to zeta potential was examined to compare flotation and sedimentation separation processes for algae harvesting. Experiments revealed that microalgae separation is related to auto-flocculation of Anabaena spp. and requires chemical coagulation for the whole period of microalgae cultivation. In addition, microalgae separation characteristics which are associated with surfactants demonstrated optimal microalgae cultivation time and separation efficiency of dissolved CO2 flotation (DCF) as an alternative to dissolved air flotation (DAF). Microalgae were significantly separated in response to anionic surfactant rather than cationic surfactant as a function of bubble size and zeta potential. DAF and DCF both showed slightly efficient flotation; however, application of anionic surfactant was required when using DCF. PMID:26287835

  19. Conversion of biomass to selected chemical products.

    PubMed

    Gallezot, Pierre

    2012-02-21

    This critical review provides a survey illustrated by recent references of different strategies to achieve a sustainable conversion of biomass to bioproducts. Because of the huge number of chemical products that can be potentially manufactured, a selection of starting materials and targeted chemicals has been done. Also, thermochemical conversion processes such as biomass pyrolysis or gasification as well as the synthesis of biofuels were not considered. The synthesis of chemicals by conversion of platform molecules obtained by depolymerisation and fermentation of biopolymers is presently the most widely envisioned approach. Successful catalytic conversion of these building blocks into intermediates, specialties and fine chemicals will be examined. However, the platform molecule value chain is in competition with well-optimised, cost-effective synthesis routes from fossil resources to produce chemicals that have already a market. The literature covering alternative value chains whereby biopolymers are converted in one or few steps to functional materials will be analysed. This approach which does not require the use of isolated, pure chemicals is well adapted to produce high tonnage products, such as paper additives, paints, resins, foams, surfactants, lubricants, and plasticisers. Another objective of the review was to examine critically the green character of conversion processes because using renewables as raw materials does not exempt from abiding by green chemistry principles (368 references). PMID:21909591

  20. Closed photobioreactors for production of microalgal biomasses.

    PubMed

    Wang, Bei; Lan, Christopher Q; Horsman, Mark

    2012-01-01

    Microalgal biomasses have been produced industrially for a long history for application in a variety of different fields. Most recently, microalgae are established as the most promising species for biofuel production and CO(2) bio-sequestration owing to their high photosynthesis efficiency. Nevertheless, design of photobioreactors that maximize solar energy capture and conversion has been one of the major challenges in commercial microalga biomass production. In this review, we systematically survey the recent developments in this field. PMID:22306165

  1. Biomass and neutral lipid production in geothermal microalgal consortia.

    PubMed

    Bywaters, Kathryn F; Fritsen, Christian H

    2014-01-01

    Recently, technologies have been developed that offer the possibility of using algal biomass as feedstocks to energy producing systems - in addition to oil-derived fuels (Bird et al., 2011, 2012). Growing native mixed microalgal consortia for biomass in association with geothermal resources has the potential to mitigate negative impacts of seasonally low temperatures on biomass production systems as well as mitigate some of the challenges associated with growing unialgal strains. We assessed community composition, growth rates, biomass, and neutral lipid production of microalgal consortia obtained from geothermal hot springs in the Great Basin/Nevada area that were cultured under different thermal and light conditions. Biomass production rates ranged from 39.0 to 344.1 mg C L(-1) day(-1). The neutral lipid production in these consortia with and without shifts to lower temperatures and additions of bicarbonate (both environmental parameters that have been shown to enhance neutral lipid production) ranged from 0 to 38.74 mg free fatty acids (FFA) and triacylglycerols (TAG) L(-1 )day(-1); the upper value was approximately 6% of the biomass produced. The higher lipid values were most likely due to the presence of Achnanthidium sp. Palmitic and stearic acids were the dominant free fatty acids. The S/U ratio (the saturated to unsaturated FA ratio) decreased for cultures shifted from their original temperature to 15°C. Biomass production was within the upper limits of those reported for individual strains, and production of neutral lipids was increased with secondary treatment. All results demonstrate a potential of culturing and manipulating resultant microalgal consortia for biomass-based energy production and perhaps even for biofuels. PMID:25763368

  2. Biomass and Neutral Lipid Production in Geothermal Microalgal Consortia

    PubMed Central

    Bywaters, Kathryn F.; Fritsen, Christian H.

    2015-01-01

    Recently, technologies have been developed that offer the possibility of using algal biomass as feedstocks to energy producing systems – in addition to oil-derived fuels (Bird et al., 2011, 2012). Growing native mixed microalgal consortia for biomass in association with geothermal resources has the potential to mitigate negative impacts of seasonally low temperatures on biomass production systems as well as mitigate some of the challenges associated with growing unialgal strains. We assessed community composition, growth rates, biomass, and neutral lipid production of microalgal consortia obtained from geothermal hot springs in the Great Basin/Nevada area that were cultured under different thermal and light conditions. Biomass production rates ranged from 39.0 to 344.1 mg C L−1 day−1. The neutral lipid production in these consortia with and without shifts to lower temperatures and additions of bicarbonate (both environmental parameters that have been shown to enhance neutral lipid production) ranged from 0 to 38.74 mg free fatty acids (FFA) and triacylglycerols (TAG) L−1 day−1; the upper value was approximately 6% of the biomass produced. The higher lipid values were most likely due to the presence of Achnanthidium sp. Palmitic and stearic acids were the dominant free fatty acids. The S/U ratio (the saturated to unsaturated FA ratio) decreased for cultures shifted from their original temperature to 15°C. Biomass production was within the upper limits of those reported for individual strains, and production of neutral lipids was increased with secondary treatment. All results demonstrate a potential of culturing and manipulating resultant microalgal consortia for biomass-based energy production and perhaps even for biofuels. PMID:25763368

  3. Investigating combustion as a method of processing inedible biomass produced in NASA's biomass production chamber

    NASA Technical Reports Server (NTRS)

    Dreschel, T. W.; Wheeler, R. M.; Hinkle, C. R.; Sager, J. C.; Knott, W. M.

    1991-01-01

    The Controlled Ecological Life Support System (CELSS) Breadboard Project at the John F. Kennedy Space Center is a research program to integrate and evaluate biological processes to provide air, water, and food for humans in closed environments for space habitation. This project focuses on the use of conventional crop plants as grown in the Biomass Production Chamber (BPC) for the production and recycling of oxygen, food, and water. The inedible portion of these crops has the potential to be converted to edible biomass or directly to the elemental constituents for direct recycling. Converting inedible biomass directly, by combustion, to carbon dioxide, water, and minerals could provide a baseline for estimating partitioning of the mass balance during recycling in a CELSS. Converting the inedible biomass to carbon dioxide and water requires the same amount of oxygen that was produced by photosynthesis. The oxygen produced during crop growth is just equal to the oxygen required to oxidize all the biomass produced during growth. Thus, the amount of oxygen produced that is available for human consumption is in proportion to the amount of biomass actually utilized by humans. The remaining oxygen must be available to oxidize the rest of the biomass back to carbon dioxide and water or the system will not be a regenerative one.

  4. The regional environmental impact of biomass production

    SciTech Connect

    Graham, R.L.

    1994-09-01

    The objective of this paper is to present a broad overview of the potential environmental impacts of biomass energy from energy crops. The subject is complex because the environmental impact of using biomass for energy must be considered in the context of alternative energy options while the environmental impact of producing biomass from energy crops must be considered in the context of the alternative land-uses. Using biomass-derived energy can reduce greenhouse gas emissions or increase them; growing biomass energy crops can enhance soil fertility or degrade it. Without knowing the context of the biomass energy, one can say little about its specific environmental impacts. The primary focus of this paper is an evaluation of the environmental impacts of growing energy crops. I present an approach for quantitatively evaluating the potential environmental impact of growing energy crops at a regional scale that accounts for the environmental and economic context of the crops. However, to set the stage for this discussion, I begin by comparing the environmental advantages and disadvantages of biomass-derived energy relative to other energy alternatives such as coal, hydropower, nuclear power, oil/gasoline, natural gas and photovoltaics.

  5. Productivity of biomass crops as influenced by landscape position

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biomass crops have the potential to produce a variety of products for use in the expanding bioeconomy. Numerous perennial plant species have been identified to serve as dedicated and custom-tailored feedstocks for the production of bioenergy and bioproducts, while also providing numerous positive en...

  6. Biomass potential of selected grass and legume crops

    SciTech Connect

    Cherney, J.H.; Johnson, K.D.; Volenec, J.J.; Greene, D.K. )

    1991-01-01

    Optimum management strategies for herbaceous biomass crops must be investigated concurrently with the development of cost-effective conversion processes. The objective of this paper is to evaluate the agronomic feasibility of several combinations of species and management systems for producing herbaceous biomass on sites ranging from good to marginal cropland in the Midwest region of the United States. Of the perennial grasses and legumes investigated, switchgrass showed the most potential as a biomass species. It requires minimum fertilizer inputs for high yield, is very persistent, and is effective in reducing soil erosion. Sorghum double-cropped with winter annual rye was very productive but required more inputs than switchgrass. Interseeding sorghum into perennial grasses was not a viable option, due to its great dependence on environmental variables. Plant composition varied greatly across species but was not greatly affected by environment or management treatmenst.

  7. Investigation and modeling of biomass decay rate in the dark and its potential influence on net productivity of solar photobioreactors for microalga Chlamydomonas reinhardtii and cyanobacterium Arthrospira platensis.

    PubMed

    Le Borgne, François; Pruvost, Jérémy

    2013-06-01

    Biomass decay rate (BDR) in the dark was investigated for Chlamydomonas reinhardtii (microalga) and Arthrospira platensis (cyanobacterium). A specific setup based on a torus photobioreactor with online gas analysis was validated, enabling us to follow the time course of the specific BDR using oxygen monitoring and mass balance. Various operating parameters that could limit respiration rates, such as culture temperature and oxygen deprivation, were then investigated. C. reinhardtii was found to present a higher BDR in the dark than A. platensis, illustrating here the difference between eukaryotic and prokaryotic cells. In both cases, temperature proved an influential parameter, and the Arrhenius law was found to efficiently relate specific BDR to culture temperature. The utility of decreasing temperature at night to increase biomass productivity in a solar photobioreactor is also illustrated. PMID:23619140

  8. Archaebacterial Fuel Production: Methane from Biomass.

    ERIC Educational Resources Information Center

    Lennox, John E.; And Others

    1983-01-01

    Discusses microbial production of methane from biomass. Topics include methogens (bacteria producing methane), ecology of methanogenesis, methanogenesis in ruminant/nonruminant and other environments, role of methanogenesis in nature, and methane production in sewage treatment plants. Also discusses construction of methane digesters (and related…

  9. Microalgal biomass production pathways: evaluation of life cycle environmental impacts

    PubMed Central

    2013-01-01

    Background Microalgae are touted as an attractive alternative to traditional forms of biomass for biofuel production, due to high productivity, ability to be cultivated on marginal lands, and potential to utilize carbon dioxide (CO2) from industrial flue gas. This work examines the fossil energy return on investment (EROIfossil), greenhouse gas (GHG) emissions, and direct Water Demands (WD) of producing dried algal biomass through the cultivation of microalgae in Open Raceway Ponds (ORP) for 21 geographic locations in the contiguous United States (U.S.). For each location, comprehensive life cycle assessment (LCA) is performed for multiple microalgal biomass production pathways, consisting of a combination of cultivation and harvesting options. Results Results indicate that the EROIfossil for microalgae biomass vary from 0.38 to 1.08 with life cycle GHG emissions of −46.2 to 48.9 (g CO2 eq/MJ-biomass) and direct WDs of 20.8 to 38.8 (Liters/MJ-biomass) over the range of scenarios analyzed. Further anaylsis reveals that the EROIfossil for production pathways is relatively location invariant, and that algae’s life cycle energy balance and GHG impacts are highly dependent on cultivation and harvesting parameters. Contrarily, algae’s direct water demands were found to be highly sensitive to geographic location, and thus may be a constraining factor in sustainable algal-derived biofuel production. Additionally, scenarios with promising EROIfossil and GHG emissions profiles are plagued with high technological uncertainty. Conclusions Given the high variability in microalgae’s energy and environmental performance, careful evaluation of the algae-to-fuel supply chain is necessary to ensure the long-term sustainability of emerging algal biofuel systems. Alternative production scenarios and technologies may have the potential to reduce the critical demands of biomass production, and should be considered to make algae a viable and more efficient biofuel alternative

  10. Production of chemicals and fuels from biomass

    SciTech Connect

    Woods, Elizabeth; Qiao, Ming; Myren, Paul; Cortright, Randy D.; Kania, John

    2015-12-15

    Described are methods, reactor systems, and catalysts for converting biomass to fuels and chemicals in a batch and/or continuous process. The process generally involves the conversion of water insoluble components of biomass, such as hemicellulose, cellulose and lignin, to volatile C.sub.2+O.sub.1-2 oxygenates, such as alcohols, ketones, cyclic ethers, esters, carboxylic acids, aldehydes, and mixtures thereof. In certain applications, the volatile C.sub.2+O.sub.1-2 oxygenates can be collected and used as a final chemical product, or used in downstream processes to produce liquid fuels, chemicals and other products.

  11. A study of algal biomass potential in selected Canadian regions.

    SciTech Connect

    Passell, Howard David; Roach, Jesse Dillon; Klise, Geoffrey T.

    2011-11-01

    A dynamic assessment model has been developed for evaluating the potential algal biomass and extracted biocrude productivity and costs, using nutrient and water resources available from waste streams in four regions of Canada (western British Columbia, Alberta oil fields, southern Ontario, and Nova Scotia). The purpose of this model is to help identify optimal locations in Canada for algae cultivation and biofuel production. The model uses spatially referenced data across the four regions for nitrogen and phosphorous loads in municipal wastewaters, and CO{sub 2} in exhaust streams from a variety of large industrial sources. Other data inputs include land cover, and solar insolation. Model users can develop estimates of resource potential by manipulating model assumptions in a graphic user interface, and updated results are viewed in real time. Resource potential by location can be viewed in terms of biomass production potential, potential CO{sub 2} fixed, biocrude production potential, and area required. The cost of producing algal biomass can be estimated using an approximation of the distance to move CO{sub 2} and water to the desired land parcel and an estimation of capital and operating costs for a theoretical open pond facility. Preliminary results suggest that in most cases, the CO{sub 2} resource is plentiful compared to other necessary nutrients (especially nitrogen), and that siting and prospects for successful large-scale algae cultivation efforts in Canada will be driven by availability of those other nutrients and the efficiency with which they can be used and re-used. Cost curves based on optimal possible siting of an open pond system are shown. The cost of energy for maintaining optimal growth temperatures is not considered in this effort, and additional research in this area, which has not been well studied at these latitudes, will be important in refining the costs of algal biomass production. The model will be used by NRC-IMB Canada to identify

  12. Microsatellite Markers of Willow Species and Characterization of 11 Polymorphic Microsatellites for Salix eriocephala (Salicaceae), a Potential Native Species for Biomass Production in Canada

    PubMed Central

    Lauron-Moreau, Aurélien; Pitre, Frédéric E.; Brouillet, Luc; Labrecque, Michel

    2013-01-01

    Biomass produced from dedicated plantations constitutes a source of renewable energy and is expected to play an important role in several countries in the coming decades. The cultivation of woody crops such as willows therefore raises several environmental issues. In North America, several native willows are potentially interesting for biomass producers. Willow trees are diverse but few species used for environmental applications have been the object of molecular genetic studies. Based on the sequenced poplar genome, 24 microsatellite markers were assayed on five native North American willow species: Salix amygdaloides, S. discolor, S. eriocephala, S. interior and S. nigra. Polymorphic microsatellite markers were used to characterize the allele data on the shrub Salix eriocephala, a North American species with economic potential. Eleven markers amplified and confirmed the potential of this species. Analysis of samples from six populations in eastern Canada showed that all markers were variable as well as polymorphic in at least one population. The number of alleles per locus ranged from 1 to 9 (mean 2.95) and showed that these microsatellite markers can be used to assess genetic diversity of North American willow species. PMID:27137372

  13. Alfalfa -- a sustainable crop for biomass energy production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Alfalfa (Medicago sativa) has the potential to be a significant contributor to America's renewable energy future. In an alfalfa biomass energy production system, alfalfa forage would be separated into stem and leave fractions. The stems would be processed to produce energy, and the leaves would be s...

  14. Outlook for Biomass Ethanol Production and Demand

    EIA Publications

    2000-01-01

    This paper presents a midterm forecast for biomass ethanol production under three different technology cases for the period 2000 to 2020, based on projections developed from the Energy Information Administration's National Energy Modeling System. An overview of cellulose conversion technology and various feedstock options and a brief history of ethanol usage in the United States are also presented.

  15. Assessment of technology for production of liquid fuels from biomass

    SciTech Connect

    Sheppard, A.P.; Spurlock, J.M.; Birchfield, J.L.

    1981-01-01

    Technologies for liquid fuel production from biomass vary widely in states of development and extent of need for government action. Ethanol produced from grain (principally corn), for use in gasohol blends, is the most widely used and accepted biomass-based energy source in the U.S. at present. Several practical factors strongly point to needed government emphasis on research and development to advance ethanol-production technology. Liquid fuels produced from soybeans, sunflowers, Euphorbia and similar crops, or from aquatic plants, remain as longer-term potential requiring further assessment. 6 refs.

  16. Hydrogen Production Cost Estimate Using Biomass Gasification: Independent Review

    SciTech Connect

    Ruth, M.

    2011-10-01

    This independent review is the conclusion arrived at from data collection, document reviews, interviews and deliberation from December 2010 through April 2011 and the technical potential of Hydrogen Production Cost Estimate Using Biomass Gasification. The Panel reviewed the current H2A case (Version 2.12, Case 01D) for hydrogen production via biomass gasification and identified four principal components of hydrogen levelized cost: CapEx; feedstock costs; project financing structure; efficiency/hydrogen yield. The panel reexamined the assumptions around these components and arrived at new estimates and approaches that better reflect the current technology and business environments.

  17. Bird Communities and Biomass Yields in Potential Bioenergy Grasslands

    PubMed Central

    Blank, Peter J.; Sample, David W.; Williams, Carol L.; Turner, Monica G.

    2014-01-01

    Demand for bioenergy is increasing, but the ecological consequences of bioenergy crop production on working lands remain unresolved. Corn is currently a dominant bioenergy crop, but perennial grasslands could produce renewable bioenergy resources and enhance biodiversity. Grassland bird populations have declined in recent decades and may particularly benefit from perennial grasslands grown for bioenergy. We asked how breeding bird community assemblages, vegetation characteristics, and biomass yields varied among three types of potential bioenergy grassland fields (grass monocultures, grass-dominated fields, and forb-dominated fields), and assessed tradeoffs between grassland biomass production and bird habitat. We also compared the bird communities in grassland fields to nearby cornfields. Cornfields had few birds compared to perennial grassland fields. Ten bird Species of Greatest Conservation Need (SGCN) were observed in perennial grassland fields. Bird species richness and total bird density increased with forb cover and were greater in forb-dominated fields than grass monocultures. SGCN density declined with increasing vertical vegetation density, indicating that tall, dense grassland fields managed for maximum biomass yield would be of lesser value to imperiled grassland bird species. The proportion of grassland habitat within 1 km of study sites was positively associated with bird species richness and the density of total birds and SGCNs, suggesting that grassland bioenergy fields may be more beneficial for grassland birds if they are established near other grassland parcels. Predicted total bird density peaked below maximum biomass yields and predicted SGCN density was negatively related to biomass yields. Our results indicate that perennial grassland fields could produce bioenergy feedstocks while providing bird habitat. Bioenergy grasslands promote agricultural multifunctionality and conservation of biodiversity in working landscapes. PMID:25299593

  18. Superior cottonwood and eucalyptus clones for biomass production in wastewater biomass production in wastewater bioremediation systems

    SciTech Connect

    Rockwood, D.L.; Pisano, S.M.; McConnell, W.V.

    1996-12-31

    Fast-growing cottonwood and Eucalyptus species have wastewater bioremediation potential. To estimate genetic variation in cottonwood`s response to sewage effluent, 10 clones were planted at Tallahassee in April 1992. Progenies and/or clones of E. Ampligolia (EA). E. Camaldulensis (EC), and E. Grandis (EG) were planted in a dry stormwater retention/bioremediation pond constructed in June 1993 at Tampa. Genetic variability within cottonwood and Eucalyptus species was observed and should be utilized to optimize biomass production and nutrient uptake in wastewater bioremediation applications. On good sites with freeze risk in northern Florida, three cottonwood clones are particularly productive. While as many as four EC and EG clones are promising, one EG clone appears superior for stormwater remediation, systems in central Florida.

  19. Root crops and their biomass potential in Florida

    SciTech Connect

    O'Hair, S.K.; Locascio, S.J.; Forbes, R.R.; White, J.M.; Hensel, D.R.; Shumaker, J.R.; Dangler, J.M.

    1983-01-01

    Root and tuber crops are of particular interest as biofuel crops because of their ability to concentrate and store fermentables including starch and sugars, in enlarged organs at or below the soil surface. In Florida, harvest index, the storage organ biomass divided by total plant biomass, of sweet potato, fodder beet, cassava and potato has approached 0.80. Chicory, fodder beet, cassava and sweet potato produced a total plant yield of 16.0, 14.1, 11.4 and 11.3 t/ha, respectively. Since the crops vary for time to maturity and storage organ chemical composition, a conventional unit to equate yield differences is kilocalorie (kcal) production/ha/day. Of the warm season crops, sweet potato and cassava roots produced an estimated 32 and 14 x 10/sup 4/ kcal/ha/day, respectively. Chinese radish and rutabaga roots produced 18 and 17 x 10/sup 4/ kcal/ha/day. Thus, a year round average of as much as 25 x 10/sup 4/ kcal/ha/day has been demonstrated. In conjunction with the total potential biomass production by a plant, root and tuber crops may be able to surpass grain crops in fermentable productivity on a temporal and spacial basis. The factors that will contribute to this include developing the appropriate cultural practices for biomass production along with breeding and selecting for adaptability and favorable harvest index. Since many of these crops have been neglected from a research standpoint, there is little doubt that improvements can be made by further work. 27 references.

  20. Sophorolipid production from biomass hydrolysates.

    PubMed

    Samad, Abdul; Zhang, Ji; Chen, Da; Liang, Yanna

    2015-02-01

    Although extensive research has been conducted on producing sophorolipids using Candida (Starmerella) bombicola from pure sugars and various oil sources, production of this biosurfactant has not been evaluated when cells are cultivated in lignocellulosic hydrolysates. Here, we report for the first time that C. bombicola is capable of producing sophorolipids on hydrolysates derived from sweet sorghum bagasse and corn fiber. Without oil supplementation, a sophorolipid concentration of 3.6 and 1.0 g/L was detected from cultures with bagasse and corn fiber hydrolysates, respectively. With the addition of soybean oil at 100 g/L, the yield of sophorolipids from these two hydrolysates in the same order was 84.6 and 15.6 g/L. Surprisingly, C. bombicola consumed all monomeric sugars and nonsugar compounds in the hydrolysates, and cultures with bagasse hydrolysates had higher yield of sophorolipids than those from a standard medium which contained pure glucose at the same concentration. PMID:25475889

  1. Forest harvesting reduces the soil metagenomic potential for biomass decomposition.

    PubMed

    Cardenas, Erick; Kranabetter, J M; Hope, Graeme; Maas, Kendra R; Hallam, Steven; Mohn, William W

    2015-11-01

    Soil is the key resource that must be managed to ensure sustainable forest productivity. Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition. From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb. A method to analyze unassembled metagenome reads from the complex community was optimized and validated. The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively. Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting. Gene families were disproportionately affected, and we identified 41 gene families consistently affected by harvesting, including families involved in lignin, cellulose, hemicellulose and pectin degradation. The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration. Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests. PMID:25909978

  2. Energy-efficient photobioreactor configuration for algal biomass production.

    PubMed

    Pegallapati, Ambica Koushik; Arudchelvam, Yalini; Nirmalakhandan, Nagamany

    2012-12-01

    An internally illuminated photobioreactor (IIPBR) design is proposed for energy-efficient biomass production. Theoretical rationale of the IIPBR design and its advantages over the traditional bubble column photobioreactors (PBRs) are presented, followed by experimental results from prototype scale cultivation of freshwater and marine algal strains in an 18L IIPBR. Based on theoretical considerations, the proposed IIPBR design has the potential to support 160% higher biomass density and higher biomass productivity per unit energy input, B/E, than a bubble column PBR of equal incident area per unit culture volume. Experimental B/E values recorded in this study with fresh water algae and marine algae (1.42 and 0.37 gW(-1)d(-1), respectively) are at least twice as those reported in the literature for comparable species cultivated in bubble column and airlift PBRs. PMID:23079413

  3. Biomass production by fescue and switchgrass alone and in mixed swards with legumes. Final project report

    SciTech Connect

    Collins, M.

    1994-06-01

    In assessing the role of biomass in alleviating potential global warming, the absence of information on the sustainability of biomass production on soils of limited agricultural potential is cited as a major constraint to the assessment of the role of biomass. Research on the sustainability of yields, recycling of nutrients, and emphasis on reduced inputs of agricultural chemicals in the production of biomass are among the critical research needs to clarify optimum cropping practice in biomass production. Two field experiments were conducted between 1989 and 1993. One study evaluated biomass production and composition of switchgrass (Panicum virgatum L.) grown alone and with bigflower vetch (Vicia grandiflora L.) and the other assessed biomass productivity and composition of tall fescue (Festuca arundinacea Schreb.) grown alone and with perennial legumes. Switchgrass received 0, 75 or 150 kg ha{sup {minus}1} of N annually as NH{sub 4}NO{sub 3} or was interseeded with vetch. Tall fescue received 0, 75, 150 or 225 kg ha{sup {minus}1} of N annually or was interseeded with alfalfa (Medicago L.) or birdsfoot trefoil (Lotus corniculatus L.). It is hoped that production systems can be designed to produce high yields of biomass with minimal inputs of fertilizer N. Achievement of this goal would reduce the potential for movement of NO{sub 3} and other undesirable N forms outside the biomass production system into the environment. In addition, management systems involving legumes could reduce the cost of biomass production.

  4. Energy production from biomass (Part 2): Conversion technologies.

    PubMed

    McKendry, Peter

    2002-05-01

    The use of biomass to provide energy has been fundamental to the development of civilisation. In recent times pressures on the global environment have led to calls for an increased use of renewable energy sources, in lieu of fossil fuels. Biomass is one potential source of renewable energy and the conversion of plant material into a suitable form of energy, usually electricity or as a fuel for an internal combustion engine, can be achieved using a number of different routes, each with specific pros and cons. A brief review of the main conversion processes is presented, with specific regard to the production of a fuel suitable for spark ignition gas engines. PMID:12058830

  5. The potential for biomass to mitigate greenhouse gas emissions in the Northeastern US. Northeast Regional Biomass Program

    SciTech Connect

    Bernow, S.S.; Gurney, K.; Prince, G.; Cyr, M.

    1992-04-01

    This study, for the Northeast Regional Biomass Program (NRBP) of the Coalition of Northeast Governors (CONEG), evaluates the potential for local, state and regional biomass policies to contribute to an overall energy/biomass strategy for the reduction of greenhouse gas releases in the Northeastern United States. Biomass is a conditionally renewable resource that can play a dual role: by reducing emissions of greenhouse gases in meeting our energy needs; and by removing carbon from the atmosphere and sequestering it in standing biomass stocks and long-lived products. In this study we examine the contribution of biomass to the energy system in the Northeast and to the region`s net releases of carbon dioxide and methane, and project these releases over three decades, given a continuation of current trends and policies. We then compare this Reference Case with three alternative scenarios, assuming successively more aggressive efforts to reduce greenhouse gas emissions through strategic implementation of energy efficiency and biomass resources. Finally, we identify and examine policy options for expanding the role of biomass in the region`s energy and greenhouse gas mitigation strategies.

  6. A 'breadboard' biomass production chamber for CELSS

    NASA Technical Reports Server (NTRS)

    Prince, Ralph P.; Knott, William M., III; Hilding, Suzanne E.; Mack, Tommy L.

    1987-01-01

    The Breadboard Project of the Controlled Ecological Life Support System (CELSS) Program is the first attempt by NASA to integrate the primary components of a bioregenerative life support system into a functioning system. The central component of this project is a Biomass Production Chamber (BPC). The BPC is under construction, and when finished will be sealed for the study of the flux of gases, liquids, and solids through the production module of a CELSS. Features of the CELSS breadboard facility will be covered as will design requirements for the BPC. Cultural practices developed for wheat for the BPC wil be discussed.

  7. Biogas production from anaerobic digestion of Spirulina maxima algal biomass

    SciTech Connect

    Samson, R.; LeDuy, A.

    1982-08-01

    The photosynthetic spectrum of solar energy could be exploited for the production of chemical energy of methane through the combined algal-bacterial process. In this process, the algae are mass produced from light and from carbon in the first step. The algal biomass is then used as a nutrient for feeding the anaerobic digester, in the second step, for the production of methane by anaerobic bacteria. The carbon source for the production of algal biomass could be either organic carbon from wastewaters (for eucaryotic algae), or carbon dioxide from the atmosphere or from the combustion exhaust gases (for both prokaryotic and eukaryotic algae). The technical feasibility data on the anaerobic digestion of algal biomass have been reported for many species of algae including macroscopic algae and microscopic algae. Research being conducted in the authors' laboratory consists of using the semimicroscopic blue-green alga Spirulina maxima as the sole substrate for this combined algal-bacterial process. This species of alga is very attractive for the process because of its capability of using the atmospheric carbon dioxide as carbon source and its simple harvesting methods. Furthermore, it appeared that the fermentability of S. maxima is significantly higher than other microscopic algae. This communication presents the results on the anaerobic inoculum development by the adaptation technique. This inoculum was then used for the semicontinuous anaerobic digestion of S. maxima algal biomass. The evolutions of biogas production and composition, biogas yield, total volatile fatty acids, alkalinity, ammonia nitrogen, pH, and electrode potential were followed.

  8. New market potential: Torrefaction of woody biomass

    SciTech Connect

    Tumuluru, Jaya Shankar; Hess, J. Richard

    2015-06-02

    Biomass was the primary source of energy worldwide until a few generations ago, when the energy-density, storability and transportability of fossil fuels enabled one of the most rapid cultural transformations in the history of humankind: the industrial revolution. In just a few hundred years, coal, oil and natural gas have prompted the development of highly efficient, high-volume manufacturing and transportation systems that have become the foundation of the world economy. But over-reliance on fossil resources has also led to environmental and energy security concerns. In addition, one of the greatest advantages of using biomass to replace fossil fuels is reduced greenhouse gas emissions and carbon footprint.

  9. Techno Economic Analysis of Hydrogen Production by gasification of biomass

    SciTech Connect

    Francis Lau

    2002-12-01

    Biomass represents a large potential feedstock resource for environmentally clean processes that produce power or chemicals. It lends itself to both biological and thermal conversion processes and both options are currently being explored. Hydrogen can be produced in a variety of ways. The majority of the hydrogen produced in this country is produced through natural gas reforming and is used as chemical feedstock in refinery operations. In this report we will examine the production of hydrogen by gasification of biomass. Biomass is defined as organic matter that is available on a renewable basis through natural processes or as a by-product of processes that use renewable resources. The majority of biomass is used in combustion processes, in mills that use the renewable resources, to produce electricity for end-use product generation. This report will explore the use of hydrogen as a fuel derived from gasification of three candidate biomass feedstocks: bagasse, switchgrass, and a nutshell mix that consists of 40% almond nutshell, 40% almond prunings, and 20% walnut shell. In this report, an assessment of the technical and economic potential of producing hydrogen from biomass gasification is analyzed. The resource base was assessed to determine a process scale from feedstock costs and availability. Solids handling systems were researched. A GTI proprietary gasifier model was used in combination with a Hysys(reg. sign) design and simulation program to determine the amount of hydrogen that can be produced from each candidate biomass feed. Cost estimations were developed and government programs and incentives were analyzed. Finally, the barriers to the production and commercialization of hydrogen from biomass were determined. The end-use of the hydrogen produced from this system is small PEM fuel cells for automobiles. Pyrolysis of biomass was also considered. Pyrolysis is a reaction in which biomass or coal is partially vaporized by heating. Gasification is a more

  10. Microbial biomass and productivity in seagrass beds

    NASA Technical Reports Server (NTRS)

    Moriarty, D. J.; Boon, P. I.; Hansen, J. A.; Hunt, W. G.; Poiner, I. R.; Pollard, P. C.; Skyring, G. W.; White, D. C.

    1985-01-01

    Different methods for measuring the rates of processes mediated by bacteria in sediments and the rates of bacterial cell production have been compared. In addition, net production of the seagrass Zostera capricorni and bacterial production have been compared and some interrelationships with the nitrogen cycle discussed. Seagrass productivity was estimated by measuring the plastochrone interval using a leaf stapling technique. The average productivity over four seasons was 1.28 +/- 0.28 g C m-2 day-1 (mean +/- standard deviation, n = 4). Bacterial productivity was measured five times throughout a year using the rate of tritiated thymidine incorporated into DNA. Average values were 33 +/- 12 mg C m-2 day-1 for sediment and 23 +/- 4 for water column (n = 5). Spatial variability between samples was greater than seasonal variation for both seagrass productivity and bacterial productivity. On one occasion, bacterial productivity was measured using the rate of 32P incorporated into phospholipid. The values were comparable to those obtained with tritiated thymidine. The rate of sulfate reduction was 10 mmol SO4(-2) m-2 day-1. The rate of methanogenesis was low, being 5.6 mg CH4 produced m-2 day-1. A comparison of C flux measured using rates of sulfate reduction and DNA synthesis indicated that anaerobic processes were predominant in these sediments. An analysis of microbial biomass and community structure, using techniques of phospholipid analysis, showed that bacteria were predominant members of the microbial biomass and that of these, strictly anaerobic bacteria were the main components. Ammonia concentration in interstitial water varied from 23 to 71 micromoles. Estimates of the amount of ammonia required by seagrass showed that the ammonia would turn over about once per day. Rapid recycling of nitrogen by bacteria and bacterial grazers is probably important.

  11. Evaluation of potential herbaceous biomass crops on marginal crop lands: 1, Agronomic potential

    SciTech Connect

    Cherney, J.H.; Johnson, K.D.; Volenec, J.J.; Kladivko, E.J.; Greene, D.K. . Dept. of Agronomy)

    1990-07-01

    Management of herbaceous biomass crops must be investigated concurrently with the development of cost-effective conversion processes. Our objective was to evaluate the agronomic feasibility of several combinations of species and management systems for producing herbaceous biomass on sites ranging from good to marginal cropland. Soil organic matter and aggregate stability were not adversely affected by any of the management options. Of the perennial grasses and legumes investigated, switchgrass showed the most potential as a biomass candidate. It has minimum fertilizer inputs, is very persistent, and is effective in reducing soil erosion. Sorghum double-cropped with winter annual rye was very productive, but required more inputs than switchgrass. Interseeding sorghum into perennial grasses was not a viable option, due to its great dependence on environmental variables. Photoperiod-sensitive and hybrid sorghums able to utilize an entire growing season were very productive, with yields up to 39 Mg ha{sup {minus}1}. Two harvest per season resulted in low yields, such that lodging resistance must be incorporated into desirable genotypes through breeding, instead of using multiple harvests to prevent lodging. Plant composition was greatly affected by species, with a wide range in composition across sorghum genotypes. Of all species evaluated, switchgrass and sorghum have the greatest biomass potential, and both of these species can be improved to produce higher yields of a more desirable chemical composition through plant breeding. 13 refs., 11 figs., 8 tabs.

  12. Alkane production from biomass: chemo-, bio- and integrated catalytic approaches.

    PubMed

    Deneyer, Aron; Renders, Tom; Van Aelst, Joost; Van den Bosch, Sander; Gabriëls, Dries; Sels, Bert F

    2015-12-01

    Linear, branched and cyclic alkanes are important intermediates and end products of the chemical industry and are nowadays mainly obtained from fossil resources. In search for alternatives, biomass feedstocks are often presented as a renewable carbon source for the production of fuels, chemicals and materials. However, providing a complete market for all these applications seems unrealistic due to both financial and logistic issues. Despite the very large scale of current alkane-based fuel applications, biomass definitely has the potential to offer a partial solution to the fuel business. For the smaller market of chemicals and materials, a transition to biomass as main carbon source is more realistic and even probably unavoidable in the long term. The appropriate use and further development of integrated chemo- and biotechnological (catalytic) process strategies will be crucial to successfully accomplish this petro-to-bio feedstock transition. Furthermore, a selection of the most promising technologies from the available chemo- and biocatalytic tool box is presented. New opportunities will certainly arise when multidisciplinary approaches are further explored in the future. In an attempt to select the most appropriate biomass sources for each specific alkane-based application, a diagram inspired by van Krevelen is applied, taking into account both the C-number and the relative functionality of the product molecules. PMID:26360875

  13. Environmental control perspective for ethanol production from biomass

    SciTech Connect

    Collins, F.J.; Dock, J.S.; Malloy, M.C.; McNulty, W.B.; Rosen, L.A.; Simons, J.R.; Tomlinson, J.C.

    1980-08-01

    This report presents the results of a study identifying the environmental control technology issues associated with the expanded production of anhydrous ethanol from biomass. It provides an analysis of the environmental control aspects of biomass production and its subsequent conversion to ethanol and a perspective on associated environmental legislation. Two potential crops were selected as representative feedstocks to focus on the generic problems associated with an expanded ethanol production program. The primary feedstock evaluated was corn, and the secondary crop considered was sugar beets. Corn and sugar beets were chosen as representative starch and sugar crops, respectively, because they can be produced in large quantities and are grown in many regions of the contiguous United States. Minimum consideration was given to agricultural and food processing wastes because of their limited availability and poor handling characteristics for transportation. Cellulosic resources were not considered because the required conversion is not commercially available in the near term. (176 refs., 10 figs., 34 tabs.)

  14. Harnessing the potential of ligninolytic enzymes for lignocellulosic biomass pretreatment.

    PubMed

    Masran, Ruqayyah; Zanirun, Zuraidah; Bahrin, Ezyana Kamal; Ibrahim, Mohamad Faizal; Lai Yee, Phang; Abd-Aziz, Suraini

    2016-06-01

    Abundant lignocellulosic biomass from various industries provides a great potential feedstock for the production of value-added products such as biofuel, animal feed, and paper pulping. However, low yield of sugar obtained from lignocellulosic hydrolysate is usually due to the presence of lignin that acts as a protective barrier for cellulose and thus restricts the accessibility of the enzyme to work on the cellulosic component. This review focuses on the significance of biological pretreatment specifically using ligninolytic enzymes as an alternative method apart from the conventional physical and chemical pretreatment. Different modes of biological pretreatment are discussed in this paper which is based on (i) fungal pretreatment where fungi mycelia colonise and directly attack the substrate by releasing ligninolytic enzymes and (ii) enzymatic pretreatment using ligninolytic enzymes to counter the drawbacks of fungal pretreatment. This review also discusses the important factors of biological pretreatment using ligninolytic enzymes such as nature of the lignocellulosic biomass, pH, temperature, presence of mediator, oxygen, and surfactant during the biodelignification process. PMID:27115758

  15. Potential for electricity generation from biomass residues in Cuba

    SciTech Connect

    Lora, E.S.

    1995-11-01

    The purpose of this paper is the study of the availability of major biomass residues in Cuba and the analysis of the electricity generation potential by using different technologies. An analysis of the changes in the country`s energy balance from 1988 up to date is presented, as well as a table with the availability study results and the energy equivalent for the following biomass residues: sugar cane bagasse and trash, rice and coffee husk, corn an cassava stalks and firewood. A total equivalent of 4.42 10{sup 6} tons/year of fuel-oil was obtained. Possible scenarios for the electricity production increase in the sugar industry are presented too. The analysis is carried out for a high stream parameter CEST and two BIG/GT system configurations. Limitations are introduced about the minimal milling capacity of the sugar mills for each technology. The calculated {open_quotes}real{close_quotes} electricity generation potential for BIG/GT systems, based on GE LM5000 CC gas turbines, an actual cane harvest of 58.0 10{sup 6} tons/year, half the available trash utilization and an specific steam consumption of 210 kg/tc, was 18601,0 GWh/year. Finally different alternatives are presented for low-scale electricity generation based on the other available agricultural residues.

  16. Wood biomass: The potential of willow. Progress report, November 1987--December 1990

    SciTech Connect

    White, E.H.; Abrahamson, L.P.

    1991-10-01

    Experiments were established in central New York State in spring, 1987, to evaluate the potential of Salix for wood biomass production using ultrashort-rotation intensive-culture techniques. Five selected willow clones and one hybrid poplar clone planted at 1 {times} 1 foot spacing were tested for biomass production with annual coppicing. This report presents results of this research as of December 31, 1990. (VC)

  17. Co-product recovery from biomass during ethanol production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The conversion of biomass to ethanol represents a sustainable alternative liquid fuel technology that does not need to compete with the supply of commodity crops such as corn and soybeans. Maintaining agricultural production of edible crops for the food supply and using agricultural waste or low inp...

  18. Development of biocatalysts for production of commodity chemicals from lignocellulosic biomass.

    PubMed

    Adsul, M G; Singhvi, M S; Gaikaiwari, S A; Gokhale, D V

    2011-03-01

    Lignocellulosic biomass is recognized as potential sustainable source for production of power, biofuels and variety of commodity chemicals which would potentially add economic value to biomass. Recalcitrance nature of biomass is largely responsible for the high cost of its conversion. Therefore, it is necessary to introduce some cost effective pretreatment processes to make the biomass polysaccharides easily amenable to enzymatic attack to release mixed fermentable sugars. Advancement in systemic biology can provide new tools for the development of such biocatalysts for sustainable production of commodity chemicals from biomass. Integration of functional genomics and system biology approaches may generate efficient microbial systems with new metabolic routes for production of commodity chemicals. This paper provides an overview of the challenges that are faced by the processes converting lignocellulosic biomass to commodity chemicals. The critical factors involved in engineering new microbial biocatalysts are also discussed with more emphasis on commodity chemicals. PMID:21277771

  19. (Assessment of the potential of Yunnan Province, China to grow and convert biomass to electricity)

    SciTech Connect

    Perlack, R.D.

    1990-10-15

    The purpose of the trip was to conduct a preliminary evaluation of biomass energy development in Yunnan Province, China. The evaluation included an assessment of the potential to grow and convert biomass to electricity, and an evaluation of the institutional relationships, which would be critical to the establishment of a collaborative biomass energy development project. This site visit was undertaken to evaluate the potential of an integrated biomass energy project, including the growing and handling of biomass feedstocks and its conversion to electricity. Based on this site visit, it was concluded that biomass production risks are real and further research on species screening and experiments is necessary before proceeding to the conversion phase of this project. The location of potential sites inspected and the logistics required for handling and transporting biomass may also be a concern. The commitment of support (labor and land) and leadership to this project by the Chinese is overwhelming exceeding all pre-site visit expectations. In sum, there is a definite opportunity in Yunnan for an integrated biomass energy project and a potential market for US technology.

  20. Biomass Biorefinery for the production of Polymers and Fuels

    SciTech Connect

    Dr. Oliver P. Peoples

    2008-05-05

    The conversion of biomass crops to fuel is receiving considerable attention as a means to reduce our dependence on foreign oil imports and to meet future energy needs. Besides their use for fuel, biomass crops are an attractive vehicle for producing value added products such as biopolymers. Metabolix, Inc. of Cambridge proposes to develop methods for producing biodegradable polymers polyhydroxyalkanoates (PHAs) in green tissue plants as well as utilizating residual plant biomass after polymer extraction for fuel generation to offset the energy required for polymer extraction. The primary plant target is switchgrass, and backup targets are alfalfa and tobacco. The combined polymer and fuel production from the transgenic biomass crops establishes a biorefinery that has the potential to reduce the nation’s dependence on foreign oil imports for both the feedstocks and energy needed for plastic production. Concerns about the widespread use of transgenic crops and the grower’s ability to prevent the contamination of the surrounding environment with foreign genes will be addressed by incorporating and expanding on some of the latest plant biotechnology developed by the project partners of this proposal. This proposal also addresses extraction of PHAs from biomass, modification of PHAs so that they have suitable properties for large volume polymer applications, processing of the PHAs using conversion processes now practiced at large scale (e.g., to film, fiber, and molded parts), conversion of PHA polymers to chemical building blocks, and demonstration of the usefulness of PHAs in large volume applications. The biodegradability of PHAs can also help to reduce solid waste in our landfills. If successful, this program will reduce U.S. dependence on imported oil, as well as contribute jobs and revenue to the agricultural economy and reduce the overall emissions of carbon to the atmosphere.

  1. Recent advances in production of succinic acid from lignocellulosic biomass.

    PubMed

    Akhtar, Junaid; Idris, Ani; Abd Aziz, Ramlan

    2014-02-01

    Production of succinic acid via separate enzymatic hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) are alternatives and are environmentally friendly processes. These processes have attained considerable positions in the industry with their own share of challenges and problems. The high-value succinic acid is extensively used in chemical, food, pharmaceutical, leather and textile industries and can be efficiently produced via several methods. Previously, succinic acid production via chemical synthesis from petrochemical or refined sugar has been the focus of interest of most reviewers. However, these expensive substrates have been recently replaced by alternative sustainable raw materials such as lignocellulosic biomass, which is cheap and abundantly available. Thus, this review focuses on succinic acid production utilizing lignocellulosic material as a potential substrate for SSF and SHF. SSF is an economical single-step process which can be a substitute for SHF - a two-step process where biomass is hydrolyzed in the first step and fermented in the second step. SSF of lignocellulosic biomass under optimum temperature and pH conditions results in the controlled release of sugar and simultaneous conversion into succinic acid by specific microorganisms, reducing reaction time and costs and increasing productivity. In addition, main process parameters which influence SHF and SSF processes such as batch and fed-batch fermentation conditions using different microbial strains are discussed in detail. PMID:24292125

  2. Yeast Biomass Production in Brewery's Spent Grains Hemicellulosic Hydrolyzate

    NASA Astrophysics Data System (ADS)

    Duarte, Luís C.; Carvalheiro, Florbela; Lopes, Sónia; Neves, Ines; Gírio, Francisco M.

    Yeast single-cell protein and yeast extract, in particular, are two products which have many feed, food, pharmaceutical, and biotechnological applications. However, many of these applications are limited by their market price. Specifically, the yeast extract requirements for culture media are one of the major technical hurdles to be overcome for the development of low-cost fermentation routes for several top value chemicals in a biorefinery framework. A potential biotechnical solution is the production of yeast biomass from the hemicellulosic fraction stream. The growth of three pentose-assimilating yeast cell factories, Debaryomyces hansenii, Kluyveromyces marxianus, and Pichia stipitis was compared using non-detoxified brewery's spent grains hemicellulosic hydrolyzate supplemented with mineral nutrients. The yeasts exhibited different specific growth rates, biomass productivities, and yields being D. hansenii as the yeast species that presented the best performance, assimilating all sugars and noteworthy consuming most of the hydrolyzate inhibitors. Under optimized conditions, D. hansenii displayed a maximum specific growth rate, biomass yield, and productivity of 0.34 h-1, 0.61 g g-1, and 0.56 g 1-1 h-1, respectively. The nutritional profile of D. hansenii was thoroughly evaluated, and it compares favorably to others reported in literature. It contains considerable amounts of some essential amino acids and a high ratio of unsaturated over saturated fatty acids.

  3. Research in biomass production and utilization: Systems simulation and analysis

    NASA Astrophysics Data System (ADS)

    Bennett, Albert Stewart

    There is considerable public interest in developing a sustainable biobased economy that favors support of family farms and rural communities and also promotes the development of biorenewable energy resources. This study focuses on a number of questions related to the development and exploration of new pathways that can potentially move us toward a more sustainable biobased economy. These include issues related to biomass fuels for drying grain, economies-of-scale, new biomass harvest systems, sugar-to-ethanol crop alternatives for the Upper Midwest U.S., biomass transportation, post-harvest biomass processing and double cropping production scenarios designed to maximize biomass feedstock production. The first section of this study considers post-harvest drying of shelled corn grain both at farm-scale and at larger community-scaled installations. Currently, drying of shelled corn requires large amounts of fossil fuel energy. To address future energy concerns, this study evaluates the potential use of combined heat and power systems that use the combustion of corn stover to produce steam for drying and to generate electricity for fans, augers, and control components. Because of the large capital requirements for solid fuel boilers and steam turbines/engines, both farm-scale and larger grain elevator-scaled systems benefit by sharing boiler and power infrastructure with other processes. The second and third sections evaluate sweet sorghum as a possible "sugarcane-like" crop that can be grown in the Upper Midwest. Various harvest systems are considered including a prototype mobile juice harvester, a hypothetical one-pass unit that separates grain heads from chopped stalks and traditional forage/silage harvesters. Also evaluated were post-harvest transportation, storage and processing costs and their influence on the possible use of sweet sorghum as a supplemental feedstock for existing dry-grind ethanol plants located in the Upper Midwest. Results show that the concept

  4. Perspectives on the economic analysis of ethanol production from biomass

    SciTech Connect

    Prebluda, H.J.; Williams, R.

    1981-01-01

    The potential and economics of ethanol production from biomass is examined. Among the topics covered are: the Brazilian gasohol program; the effect of large scale conversion of grain to alcohol on U.S. food and animal feed prices; the Ex-Ferm process for fermenting sugar cane; the effect on cane sugar markets of the large-scale development in the U.S. of high fructose corn syrup; and better utilization of by-products. Significant breakthroughs which have recently taken place and which will improve the economic picture for making alcohol from solid waste are reviewed. (Refs. 26).

  5. Liquid fuels production from biomass. Final report

    SciTech Connect

    Levy, P. F.; Sanderson, J. E.; Ashare, E.; Wise, D. L.; Molyneaux, M. S.

    1980-06-30

    The current program to convert biomass into liquid hydrocarbon fuels is an extension of a previous program to ferment marine algae to acetic acid. In that study it was found that marine algae could be converted to higher aliphatic organic acids and that these acids could be readily removed from the fermentation broth by membrane or liquid-liquid extraction. It was then proposed to convert these higher organic acids via Kolbe electrolysis to aliphatic hydrocarbons, which may be used as a diesel fuel. The specific goals for the current porgram are: (1) establish conditions under which substrates other than marine algae may be converted in good yield to organic acids, here the primary task is methane suppression; (2) modify the current 300-liter fixed packed bed batch fermenter to operate in a continuous mode; (3) change from membrane extraction of organic acids to liquid-liquid extraction; (4) optimize the energy balance of the electrolytic oxidation process, the primary task is to reduce the working potential required for the electrolysis while maintaining an adequate current density; (5) scale the entire process up to match the output of the 300 liter fermenter; and (6) design pilot plant and commercial size plant (1000 tons/day) processes for converting biomass to liquid hydrocarbon fuels and perform an economic analysis for the 1000 ton/day design.

  6. Potential of biomass residue availability; The case of Thailand

    SciTech Connect

    Bhattacharya, S.C.; Shrestha, R.M.; Ngamkajornvivat, S. )

    1989-01-01

    An acute shortage of fuel wood and charcoal prevails in many developing countries. A logical approach to the problem places emphasis on the development of alternative energy sources, including use of biomass residues. An assessment of the potential of biomass residues for energy and other uses calls for an estimation of their annual production. Also, because the residues are normally bulky they should be utilized near their place of origin whenever possible to avoid high transportation costs. Thus knowledge of the total national generation of residues per year does not provide enough information for planning residue utilization. This article illustrates a method of residue estimation that takes the case of Thailand as an example. It presents the annual generation of nine agricultural resides (paddy husk, paddy straw, bagasse, cotton stalk, corn cob, groundnut shell, cassava stalk and coconut husk and shell) and one forestry residue (sawdust) in different agroeconomic zones and regions of Thailand. The methodology used for the investigation of crop-to-residue ratios is outlined. The annual generation figures for the different residues along with observations about their traditional uses are presented.

  7. Potential for post-closure radionuclide redistribution due to biotic intrusion: aboveground biomass, litter production rates, and the distribution of root mass with depth at material disposal area G, Los Alamos National Laboratory

    SciTech Connect

    French, Sean B; Christensen, Candace; Jennings, Terry L; Jaros, Christopher L; Wykoff, David S; Crowell, Kelly J; Shuman, Rob

    2008-01-01

    Low-level radioactive waste (LLW) generated at the Los Alamos National Laboratories (LANL) is disposed of at LANL's Technical Area (T A) 54, Material Disposal Area (MDA) G. The ability of MDA G to safely contain radioactive waste during current and post-closure operations is evaluated as part of the facility's ongoing performance assessment (PA) and composite analysis (CA). Due to the potential for uptake and incorporation of radio nuclides into aboveground plant material, the PA and CA project that plant roots penetrating into buried waste may lead to releases of radionuclides into the accessible environment. The potential amount ofcontamination deposited on the ground surface due to plant intrusion into buried waste is a function of the quantity of litter generated by plants, as well as radionuclide concentrations within the litter. Radionuclide concentrations in plant litter is dependent on the distribution of root mass with depth and the efficiency with which radionuclides are extracted from contaminated soils by the plant's roots. In order to reduce uncertainties associated with the PA and CA for MDA G, surveys are being conducted to assess aboveground biomass, plant litter production rates, and root mass with depth for the four prominent vegetation types (grasses, forbs, shrubs and trees). The collection of aboveground biomass for grasses and forbs began in 2007. Additional sampling was conducted in October 2008 to measure root mass with depth and to collect additional aboveground biomass data for the types of grasses, forbs, shrubs, and trees that may become established at MDA G after the facility undergoes final closure, Biomass data will be used to estimate the future potential mass of contaminated plant litter fall, which could act as a latent conduit for radionuclide transport from the closed disposal area. Data collected are expected to reduce uncertainties associated with the PA and CA for MDA G and ultimately aid in the assessment and subsequent

  8. Biomass Crop Production: Benefits for Soil Quality and Carbon Sequestration

    SciTech Connect

    Bandaranayake, W.; Bock, B.R.; Houston, A.; Joslin, J.D.; Pettry, D.E.; Schoenholtz, S.; Thornton, F.C.; Tolbert, V.R.; Tyler, D.

    1999-08-29

    Research at three locations in the southeastern US is quantifying changes in soil quality and soil carbon storage that occur during production of biomass crops compared with row crops. After three growing seasons, soil quality improved and soil carbon storage increased on plots planted to cottonwood, sycamore, sweetgum with a cover crop, switchgrass, and no-till corn. For tree crops, sequestered belowground carbon was found mainly in stumps and large roots. At the TN site, the coarse woody organic matter storage belowground was 1.3 Mg ha{sup {minus}1}yr{sup {minus}1}, of which 79% was stumps and large roots and 21% fine roots. Switchgrass at the AL site also stored considerable carbon belowground as coarse roots. Most of the carbon storage occurred mainly in the upper 30 cw although coarse roots were found to depths of greater than 60 cm. Biomass crops contributed to improvements in soil physical quality as well as increasing belowground carbon sequestration. The distribution and extent of carbon sequestration depends on the growth characteristics and age of the individual biomass crop species. Time and increasing crop maturity will determine the potential of these biomass crops to significantly contribute to the overall national goal of increasing carbon sequestration and reducing greenhouse gas emissions.

  9. Biomass gasification for liquid fuel production

    SciTech Connect

    Najser, Jan E-mail: vaclav.peer@vsb.cz; Peer, Václav E-mail: vaclav.peer@vsb.cz

    2014-08-06

    In our old fix-bed autothermal gasifier we tested wood chips and wood pellets. We make experiments for Czech company producing agro pellets - pellets made from agricultural waste and fastrenewable natural resources. We tested pellets from wheat and rice straw and hay. These materials can be very perspective, because they dońt compete with food production, they were formed in sufficient quantity and in the place of their treatment. New installation is composed of allothermal biomass fixed bed gasifier with conditioning and using produced syngas for Fischer - Tropsch synthesis. As a gasifying agent will be used steam. Gas purification will have two parts - separation of dust particles using a hot filter and dolomite reactor for decomposition of tars. In next steps, gas will be cooled, compressed and removed of sulphur and chlorine compounds and carbon dioxide. This syngas will be used for liquid fuel synthesis.

  10. Biomass gasification for liquid fuel production

    NASA Astrophysics Data System (ADS)

    Najser, Jan; Peer, Václav; Vantuch, Martin

    2014-08-01

    In our old fix-bed autothermal gasifier we tested wood chips and wood pellets. We make experiments for Czech company producing agro pellets - pellets made from agricultural waste and fastrenewable natural resources. We tested pellets from wheat and rice straw and hay. These materials can be very perspective, because they dońt compete with food production, they were formed in sufficient quantity and in the place of their treatment. New installation is composed of allothermal biomass fixed bed gasifier with conditioning and using produced syngas for Fischer - Tropsch synthesis. As a gasifying agent will be used steam. Gas purification will have two parts - separation of dust particles using a hot filter and dolomite reactor for decomposition of tars. In next steps, gas will be cooled, compressed and removed of sulphur and chlorine compounds and carbon dioxide. This syngas will be used for liquid fuel synthesis.

  11. Sophorolipid production from lignocellulosic biomass feedstocks

    NASA Astrophysics Data System (ADS)

    Samad, Abdul

    , the yield of SLs was 0.55 g/g carbon (sugars plus oil) for cultures with bagasse hydrolysates. Further, SL production was investigated using sweet sorghum bagasse and corn stover hydrolysates derived from different pretreatment conditions. For the former and latter sugar sources, yellow grease or soybean oil was supplemented at different doses to enhance sophorolipid yield. 14-day batch fermentation on bagasse hydrolysates with 10, 40 and 60 g/L of yellow grease had cell densities of 5.7 g/L, 6.4 g/L and 7.8 g/L, respectively. The study also revealed that the yield of SLs on bagasse hydrolysate decreased from 0.67 to 0.61 and to 0.44 g/g carbon when yellow grease was dosed at 10, 40 and 60 g/L. With aforementioned increasing yellow grease concentration, the residual oil left after 14 days was recorded as 3.2 g/L, 8.5 g/L and 19.9 g/L. For similar experimental conditions, the cell densities observed for corn stover hydrolysate combined with soybean oil at 10, 20 and 40 g/L concentration were 6.1 g/L, 5.9 g/L, and 5.4 g/L respectively. Also, in the same order of oil dose supplemented, the residual oil recovered after 14-day was 8.5 g/L, 8.9 g/L, and 26.9 g/L. Corn stover hydrolysate mixed with the 10, 20 and 40 g/L soybean oil, the SL yield was 0.19, 0.11 and 0.09 g/g carbon. Overall, both hydrolysates supported cell growth and sophorolipid production. The results from this research show that hydrolysates derived from the different lignocellulosic biomass feedstocks can be utilized by C. bombicola to achieve substantial yields of SLs. Based upon the results revealed by several batch-stage experiments, it can be stated that there is great potential for scaling up and industrial scale production of these high value products in future.

  12. Potential for Genetic Improvement of Sugarcane as a Source of Biomass for Biofuels.

    PubMed

    Hoang, Nam V; Furtado, Agnelo; Botha, Frederik C; Simmons, Blake A; Henry, Robert J

    2015-01-01

    Sugarcane (Saccharum spp. hybrids) has great potential as a major feedstock for biofuel production worldwide. It is considered among the best options for producing biofuels today due to an exceptional biomass production capacity, high carbohydrate (sugar + fiber) content, and a favorable energy input/output ratio. To maximize the conversion of sugarcane biomass into biofuels, it is imperative to generate improved sugarcane varieties with better biomass degradability. However, unlike many diploid plants, where genetic tools are well developed, biotechnological improvement is hindered in sugarcane by our current limited understanding of the large and complex genome. Therefore, understanding the genetics of the key biofuel traits in sugarcane and optimization of sugarcane biomass composition will advance efficient conversion of sugarcane biomass into fermentable sugars for biofuel production. The large existing phenotypic variation in Saccharum germplasm and the availability of the current genomics technologies will allow biofuel traits to be characterized, the genetic basis of critical differences in biomass composition to be determined, and targets for improvement of sugarcane for biofuels to be established. Emerging options for genetic improvement of sugarcane for the use as a bioenergy crop are reviewed. This will better define the targets for potential genetic manipulation of sugarcane biomass composition for biofuels. PMID:26636072

  13. Potential for Genetic Improvement of Sugarcane as a Source of Biomass for Biofuels

    PubMed Central

    Hoang, Nam V.; Furtado, Agnelo; Botha, Frederik C.; Simmons, Blake A.; Henry, Robert J.

    2015-01-01

    Sugarcane (Saccharum spp. hybrids) has great potential as a major feedstock for biofuel production worldwide. It is considered among the best options for producing biofuels today due to an exceptional biomass production capacity, high carbohydrate (sugar + fiber) content, and a favorable energy input/output ratio. To maximize the conversion of sugarcane biomass into biofuels, it is imperative to generate improved sugarcane varieties with better biomass degradability. However, unlike many diploid plants, where genetic tools are well developed, biotechnological improvement is hindered in sugarcane by our current limited understanding of the large and complex genome. Therefore, understanding the genetics of the key biofuel traits in sugarcane and optimization of sugarcane biomass composition will advance efficient conversion of sugarcane biomass into fermentable sugars for biofuel production. The large existing phenotypic variation in Saccharum germplasm and the availability of the current genomics technologies will allow biofuel traits to be characterized, the genetic basis of critical differences in biomass composition to be determined, and targets for improvement of sugarcane for biofuels to be established. Emerging options for genetic improvement of sugarcane for the use as a bioenergy crop are reviewed. This will better define the targets for potential genetic manipulation of sugarcane biomass composition for biofuels. PMID:26636072

  14. Second generation bioethanol potential from selected Malaysia's biodiversity biomasses: A review.

    PubMed

    Aditiya, H B; Chong, W T; Mahlia, T M I; Sebayang, A H; Berawi, M A; Nur, Hadi

    2016-01-01

    Rising global temperature, worsening air quality and drastic declining of fossil fuel reserve are the inevitable phenomena from the disorganized energy management. Bioethanol is believed to clear out the effects as being an energy-derivable product sourced from renewable organic sources. Second generation bioethanol interests many researches from its unique source of inedible biomass, and this paper presents the potential of several selected biomasses from Malaysia case. As one of countries with rich biodiversity, Malaysia holds enormous potential in second generation bioethanol production from its various agricultural and forestry biomasses, which are the source of lignocellulosic and starch compounds. This paper reviews potentials of biomasses and potential ethanol yield from oil palm, paddy (rice), pineapple, banana and durian, as the common agricultural waste in the country but uncommon to be served as bioethanol feedstock, by calculating the theoretical conversion of cellulose, hemicellulose and starch components of the biomasses into bioethanol. Moreover, the potential of the biomasses as feedstock are discussed based on several reported works. PMID:26253329

  15. Liquid Fuel Production from Biomass via High Temperature Steam Electrolysis

    SciTech Connect

    Grant L. Hawkes; Michael G. McKellar

    2009-11-01

    A process model of syngas production using high temperature electrolysis and biomass gasification is presented. Process heat from the biomass gasifier is used to heat steam for the hydrogen production via the high temperature steam electrolysis process. Hydrogen from electrolysis allows a high utilization of the biomass carbon for syngas production. Oxygen produced form the electrolysis process is used to control the oxidation rate in the oxygen-fed biomass gasifier. Based on the gasifier temperature, 94% to 95% of the carbon in the biomass becomes carbon monoxide in the syngas (carbon monoxide and hydrogen). Assuming the thermal efficiency of the power cycle for electricity generation is 50%, (as expected from GEN IV nuclear reactors), the syngas production efficiency ranges from 70% to 73% as the gasifier temperature decreases from 1900 K to 1500 K. Parametric studies of system pressure, biomass moisture content and low temperature alkaline electrolysis are also presented.

  16. Feasibility of Bioethanol Production From Lignocellulosic Biomass

    NASA Astrophysics Data System (ADS)

    Aunina, Zane; Bazbauers, Gatis; Valters, Karlis

    2010-01-01

    The objective of the paper is to discuss the potential of cellulosic ethanol production processes and compare them, to find the most appropriate production method for Latvia's situation, to perform theoretical calculations and to determine the potential ethanol price. In addition, price forecasts for future cellulosic and grain ethanol are compared. A feasibility estimate to determine the price of cellulosic ethanol in Latvia, if production were started in 2010, was made. The grain and cellulosic ethanol price comparison (future forecast) was made through to the year 2018.

  17. Ethanol production from carbon dioxide using cyanobacterial biomass

    SciTech Connect

    Mustaqim, Dani; Ohtaguchi, Kazuhisa

    1996-12-31

    An ethanol production system, which consists of chemical and biochemical reaction processes for (1) biomass production using cyanobacterium Synechococcus leopoliensis through photosynthetic CO{sub 2} fixation, (2) glucose extraction from that biomass, and (3) ethanol fermentation from the extracted glucose using yeast Saccharomyces sake, was conceptually developed. S. sake was grown on the medium containing the cyanobacterial biomass extract and that containing reagent glucose. It was found that the specific rates of cell growth, glucose consumption, ethanol production and the yeast ethanol tolerance were enhanced by the addition of cyanobacterial biomass extract. 4 refs., 5 figs., 1 tab.

  18. Development Potentials and Policy Options of Biomass in China

    NASA Astrophysics Data System (ADS)

    Shen, Lei; Liu, Litao; Yao, Zhijun; Liu, Gang; Lucas, Mario

    2010-10-01

    Biomass, one of the most important renewable energies, is playing and will continue to play an important role in the future energy structure of the world. This article aims to analyze the position and role, assess the resource availability, discuss the geographic distribution, market scale and industry development, and present the policy options of biomass in China. The resource availability and geographical distribution of biomass byproducts are assessed in terms of crop residues, manure, forest and wood biomass byproducts, municipal waste and wastewater. The position of biomass use for power generation is just next to hydropower among types of renewable energy in China. The potential quantity of all biomass byproducts energy in 2004 is 3511 Mtce (Mtce is the abbreviation of million tons of coal equivalents and 1 Mtce is equal to106 tce.), while the acquirable quantity is 460 Mtce. Biomass energy plays a critical role in rural regions of China. The geographical distribution and quantity of biomass byproducts resources depends mainly on the relationship between ecological zones and climate conditions. Our estimation shows that the total quantity of crop residues, manure, forest and wood biomass byproducts, municipal waste and wastewater resources are 728, 3926, 2175, 155 and 48240 Mt (million tons), respectively. Crop residues come mainly from the provinces of Henan, Shandong, Heilongjiang, Jilin and Sichuan. All manure is mainly located in the provinces of Henan, Shandong, Sichuan, Hebei and Hunan. Forest and wood biomass byproducts are mainly produced in the provinces or autonomous regions of Tibet, Sichuan, Yunnan, Heilongjiang and Inner Mongolia, while most of municipal waste mainly comes from Guangdong, Shandong, Heilongjiang, Hubei and Jiangsu. Most of wastewater is largely discharged from advanced provinces like Guangdong, Jiangsu, Zhejiang, Shandong and Henan. Biomass byproducts’ energy distribution also varies from province to province in China. Based on

  19. Analysis of coastal upwelling and the production of a biomass

    NASA Technical Reports Server (NTRS)

    Howe, J. T.

    1979-01-01

    The coastal upwelling index derived from weather data is input to a set of coupled differential equations that describe the production of a biomass. The curl of the wind stress vector is discussed in the context of the physical extent of the upwelling structure. An analogy between temperature and biomass concentration in the upwelled coastal water is derived and the relationship is quantified. The use of remote satellite or airborne sensing to obtain biomass rate production coefficients is considered.

  20. Combined biomass valorization and hydrogen production in a photoelectrochemical cell

    NASA Astrophysics Data System (ADS)

    Cha, Hyun Gil; Choi, Kyoung-Shin

    2015-04-01

    In a typical hydrogen-producing photoelectrochemical cell (PEC), water reduction at the cathode (producing hydrogen) is accompanied by water oxidation at the anode (producing oxygen). This anode reaction is, however, not kinetically favourable. Here we investigate the possibility of utilizing solar energy for biomass conversion by performing the oxidation of 5-hydroxymethylfurfural (HMF) into 2,5-furandicarboxylic acid (FDCA) at the anode of a PEC. HMF is a key intermediate in biomass conversion, and FDCA is an important monomer for the production of numerous polymers. Using 2,2,6,6-tetramethylpiperidine-1-oxyl as a mediator, we obtained a near-quantitative yield and 100% Faradaic efficiency at ambient conditions without the use of precious-metal catalysts. This reaction is also thermodynamically and kinetically more favourable than water oxidation. Our results suggest that solar-driven biomass conversion can be a viable anode reaction that has the potential to increase both the efficiency and the utility of PECs constructed for solar-fuel production.

  1. Harvest management of switchgrass for biomass feedstock and forage production

    SciTech Connect

    Sanderson, M.A.; Read, J.C.; Reed, R.L.

    1999-02-01

    Switchgrass (Panicum virgatum L.), a warm-season perennial grass native to North America, has potential as a biomass energy crop. Their objective was to develop harvest management recommendations for biomass feedstock and forage production. Alamo switchgrass was established in 1992 at Stephenville and Dallas, TX. Four harvest frequencies (one to four cuts per year) and three final autumn harvests (Sept., Oct., or Nov.) were imposed from 1993 to 1996. Tiller densities were counted each spring. Neutral detergent fiber (NDF) and crude protein (CP) concentrations were measured in 1993 and 1994. Concentrations of NDF were lowest and of CP were highest in May-harvested biomass. Forage quality of regrowth decreased with age, reaching NDF concentrations of 790 g kg{sup {minus}1} and CP of < 20 g kg{sup {minus}1}. Total seasonal yields decreased as harvest frequency increased; however, a severe drought reversed this trend at Dallas in 1996. The highest yields occurred with a single harvest in mid-September. Delaying the final harvest until November reduced yields. Harvest date and frequency did not affect tiller density, although tiller density decreased from 900 to 650 and 630 to 310 m{sup {minus}2} at Dallas and Stephenville, respectively, during 1994 to 1997. Thus, a single mid-September harvest should maximize biomass yields in the south-central USA. A two-cut (spring-autumn) system may allow a farmer to use initial growth as forage and the regrowth for biomass, but total yields would be reduced. More frequent harvests would reduce yields further.

  2. The economic prospects of cellulosic biomass for biofuel production

    NASA Astrophysics Data System (ADS)

    Kumarappan, Subbu

    Alternative fuels for transportation have become the focus of intense policy debate and legislative action due to volatile oil prices, an unstable political environment in many major oil producing regions, increasing global demand, dwindling reserves of low-cost oil, and concerns over global warming. A major potential source of alternative fuels is biofuels produced from cellulosic biomass, which have a number of potential benefits. Recognizing these potential advantages, the Energy Independence and Security Act of 2007 has mandated 21 billion gallons of cellulosic/advanced biofuels per year by 2022. The United States needs 220-300 million tons of cellulosic biomass per year from the major sources such as agricultural residues, forestry and mill residues, herbaceous resources, and waste materials (supported by Biomass Crop Assistance Program) to meet these biofuel targets. My research addresses three key major questions concerning cellulosic biomass supply. The first paper analyzes cellulosic biomass availability in the United States and Canada. The estimated supply curves show that, at a price of 100 per ton, about 568 million metric tons of biomass is available in the United States, while 123 million metric tons is available in Canada. In fact, the 300 million tons of biomass required to meet EISA mandates can be supplied at a price of 50 per metric ton or lower. The second paper evaluates the farmers' perspective in growing new energy crops, such as switchgrass and miscanthus, in prime cropland, in pasture areas, or on marginal lands. My analysis evaluates how the farmers' returns from energy crops compare with those from other field crops and other agricultural land uses. The results suggest that perennial energy crops yielding at least 10 tons per acre annually will be competitive with a traditional corn-soybean rotation if crude oil prices are high (ranging from 88-178 per barrel over 2010-2019). If crude oil prices are low, then energy crops will not be

  3. Autohydrolysis Pretreatment of Lignocellulosic Biomass for Bioethanol Production

    NASA Astrophysics Data System (ADS)

    Han, Qiang

    Autohydrolysis, a simple and environmental friendly process, has long been studied but often abandoned as a financially viable pretreatment for bioethanol production due to the low yields of fermentable sugars at economic enzyme dosages. The introduction of mechanical refining can generate substantial improvements for autohydrolysis process, making it an attractive pretreatment technology for bioethanol commercialization. In this study, several lignocellulosic biomass including wheat straw, switchgrass, corn stover, waste wheat straw have been subjected to autohydrolysis pretreatment followed by mechanical refining to evaluate the total sugar recovery at affordable enzyme dosages. Encouraging results have been found that using autohydrolysis plus refining strategy, the total sugar recovery of most feedstock can be as high as 76% at 4 FPU/g enzymes dosages. The mechanical refining contributed to the improvement of enzymatic sugar yield by as much as 30%. Three non-woody biomass (sugarcane bagasse, wheat straw, and switchgrass) and three woody biomass (maple, sweet gum, and nitens) have been subjected to autohydrolysis pretreatment to acquire a fundamental understanding of biomass characteristics that affect the autohydrolysis and the following enzymatic hydrolysis. It is of interest to note that the nonwoody biomass went through substantial delignification during autohydrolysis compared to woody biomass due to a significant amount of p-coumaric acid and ferulic acid. It has been found that hardwood which has a higher S/V ratio in the lignin structure tends to have a higher total sugar recovery from autohydrolysis pretreatment. The economics of bioethanol production from autohydrolysis of different feedstocks have been investigated. Regardless of different feedstocks, in the conventional design, producing bioethanol and co-producing steam and power, the minimum ethanol revenues (MER) required to generate a 12% internal rate of return (IRR) are high enough to

  4. Iron nutrition, biomass production, and plant product quality.

    PubMed

    Briat, Jean-François; Dubos, Christian; Gaymard, Frédéric

    2015-01-01

    One of the grand challenges in modern agriculture is increasing biomass production, while improving plant product quality, in a sustainable way. Of the minerals, iron (Fe) plays a major role in this process because it is essential both for plant productivity and for the quality of their products. Fe homeostasis is an important determinant of photosynthetic efficiency in algae and higher plants, and we review here the impact of Fe limitation or excess on the structure and function of the photosynthetic apparatus. We also discuss the agronomic, plant breeding, and transgenic approaches that are used to remediate Fe deficiency of plants on calcareous soils, and suggest ways to increase the Fe content and bioavailability of the edible parts of crops to improve human diet. PMID:25153038

  5. The potential for biomass to mitigate greenhouse gas emissions in the Northeastern US

    SciTech Connect

    Bernow, S.S.; Gurney, K.; Prince, G.; Cyr, M.

    1992-04-01

    This study, for the Northeast Regional Biomass Program (NRBP) of the Coalition of Northeast Governors (CONEG), evaluates the potential for local, state and regional biomass policies to contribute to an overall energy/biomass strategy for the reduction of greenhouse gas releases in the Northeastern United States. Biomass is a conditionally renewable resource that can play a dual role: by reducing emissions of greenhouse gases in meeting our energy needs; and by removing carbon from the atmosphere and sequestering it in standing biomass stocks and long-lived products. In this study we examine the contribution of biomass to the energy system in the Northeast and to the region's net releases of carbon dioxide and methane, and project these releases over three decades, given a continuation of current trends and policies. We then compare this Reference Case with three alternative scenarios, assuming successively more aggressive efforts to reduce greenhouse gas emissions through strategic implementation of energy efficiency and biomass resources. Finally, we identify and examine policy options for expanding the role of biomass in the region's energy and greenhouse gas mitigation strategies.

  6. Biotechnological routes based on lactic acid production from biomass.

    PubMed

    Gao, Chao; Ma, Cuiqing; Xu, Ping

    2011-01-01

    Lactic acid, the most important hydroxycarboxylic acid, is now commercially produced by the fermentation of sugars present in biomass. In addition to its use in the synthesis of biodegradable polymers, lactic acid can be regarded as a feedstock for the green chemistry of the future. Different potentially useful chemicals such as pyruvic acid, acrylic acid, 1,2-propanediol, and lactate ester can be produced from lactic acid via chemical and biotechnological routes. Here, we reviewed the current status of the production of potentially valuable chemicals from lactic acid via biotechnological routes. Although some of the reactions described in this review article are still not applicable at current stage, due to their "greener" properties, biotechnological processes for the production of lactic acid derivatives might replace the chemical routes in the future. PMID:21846500

  7. Unlocking the potential of lignocellulosic biomass through plant science.

    PubMed

    Marriott, Poppy E; Gómez, Leonardo D; McQueen-Mason, Simon J

    2016-03-01

    The aim of producing sustainable liquid biofuels and chemicals from lignocellulosic biomass remains high on the sustainability agenda, but is challenged by the costs of producing fermentable sugars from these materials. Sugars from plant biomass can be fermented to alcohols or even alkanes, creating a liquid fuel in which carbon released on combustion is balanced by its photosynthetic capture. Large amounts of sugar are present in the woody, nonfood parts of crops and could be used for fuel production without compromising global food security. However, the sugar in woody biomass is locked up in the complex and recalcitrant lignocellulosic plant cell wall, making it difficult and expensive to extract. In this paper, we review what is known about the major polymeric components of woody plant biomass, with an emphasis on the molecular interactions that contribute to its recalcitrance to enzymatic digestion. In addition, we review the extensive research that has been carried out in order to understand and reduce lignocellulose recalcitrance and enable more cost-effective production of fuel from woody plant biomass. PMID:26443261

  8. Production of New Biomass/Waste-Containing Solid Fuels

    SciTech Connect

    Glenn A. Shirey; David J. Akers

    2005-09-23

    CQ Inc. and its industry partners--PBS Coals, Inc. (Friedens, Pennsylvania), American Fiber Resources (Fairmont, West Virginia), Allegheny Energy Supply (Williamsport, Maryland), and the Heritage Research Group (Indianapolis, Indiana)--addressed the objectives of the Department of Energy and industry to produce economical, new solid fuels from coal, biomass, and waste materials that reduce emissions from coal-fired boilers. This project builds on the team's commercial experience in composite fuels for energy production. The electric utility industry is interested in the use of biomass and wastes as fuel to reduce both emissions and fuel costs. In addition to these benefits, utilities also recognize the business advantage of consuming the waste byproducts of customers both to retain customers and to improve the public image of the industry. Unfortunately, biomass and waste byproducts can be troublesome fuels because of low bulk density, high moisture content, variable composition, handling and feeding problems, and inadequate information about combustion and emissions characteristics. Current methods of co-firing biomass and wastes either use a separate fuel receiving, storage, and boiler feed system, or mass burn the biomass by simply mixing it with coal on the storage pile. For biomass or biomass-containing composite fuels to be extensively used in the U.S., especially in the steam market, a lower cost method of producing these fuels must be developed that is applicable to a variety of combinations of biomass, wastes, and coal; economically competitive with current fuels; and provides environmental benefits compared with coal. During Phase I of this project (January 1999 to July 2000), several biomass/waste materials were evaluated for potential use in a composite fuel. As a result of that work and the team's commercial experience in composite fuels for energy production, paper mill sludge and coal were selected for further evaluation and demonstration in Phase II

  9. Making environmental assessments of biomass production systems comparable worldwide

    NASA Astrophysics Data System (ADS)

    Meyer, Markus A.; Seppelt, Ralf; Witing, Felix; Priess, Joerg A.

    2016-03-01

    Global demand for agricultural and forestry products fundamentally affects regional land-use change associated with environmental impacts (EIs) such as erosion. In contrast to aggregated global metrics such as greenhouse gas (GHG) balances, local/regional EIs of different agricultural and forestry production regions need methods which enable worldwide EI comparisons. The key aspect is to control environmental heterogeneity to reveal man-made differences of EIs between production regions. Environmental heterogeneity is the variation in biotic and abiotic environmental conditions. In the present study, we used three approaches to control environmental heterogeneity: (i) environmental stratification, (ii) potential natural vegetation (PNV), and (iii) regional environmental thresholds to compare EIs of solid biomass production. We compared production regions of managed forests and plantation forests in subtropical (Satilla watershed, Southeastern US), tropical (Rufiji basin, Tanzania), and temperate (Mulde watershed, Central Germany) climates. All approaches supported the comparison of the EIs of different land-use classes between and within production regions. They also standardized the different EIs for a comparison between the EI categories. The EIs for different land-use classes within a production region decreased with increasing degree of naturalness (forest, plantation forestry, and cropland). PNV was the most reliable approach, but lacked feasibility and relevance. The PNV approach explicitly included most of the factors that drive environmental heterogeneity in contrast to the stratification and threshold approaches. The stratification approach allows consistent global application due to available data. Regional environmental thresholds only included arbitrarily selected aspects of environmental heterogeneity; they are only available for few EIs. Especially, the PNV and stratification approaches are options to compare regional EIs of biomass or crop production

  10. FRACTIONATION OF LIGNOCELLULOSIC BIOMASS FOR FUEL-GRADE ETHANOL PRODUCTION

    SciTech Connect

    F.D. Guffey; R.C. Wingerson

    2002-10-01

    PureVision Technology, Inc. (PureVision) of Fort Lupton, Colorado is developing a process for the conversion of lignocellulosic biomass into fuel-grade ethanol and specialty chemicals in order to enhance national energy security, rural economies, and environmental quality. Lignocellulosic-containing plants are those types of biomass that include wood, agricultural residues, and paper wastes. Lignocellulose is composed of the biopolymers cellulose, hemicellulose, and lignin. Cellulose, a polymer of glucose, is the component in lignocellulose that has potential for the production of fuel-grade ethanol by direct fermentation of the glucose. However, enzymatic hydrolysis of lignocellulose and raw cellulose into glucose is hindered by the presence of lignin. The cellulase enzyme, which hydrolyzes cellulose to glucose, becomes irreversibly bound to lignin. This requires using the enzyme in reagent quantities rather than in catalytic concentration. The extensive use of this enzyme is expensive and adversely affects the economics of ethanol production. PureVision has approached this problem by developing a biomass fractionator to pretreat the lignocellulose to yield a highly pure cellulose fraction. The biomass fractionator is based on sequentially treating the biomass with hot water, hot alkaline solutions, and polishing the cellulose fraction with a wet alkaline oxidation step. In September 2001 PureVision and Western Research Institute (WRI) initiated a jointly sponsored research project with the U.S. Department of Energy (DOE) to evaluate their pretreatment technology, develop an understanding of the chemistry, and provide the data required to design and fabricate a one- to two-ton/day pilot-scale unit. The efforts during the first year of this program completed the design, fabrication, and shakedown of a bench-scale reactor system and evaluated the fractionation of corn stover. The results from the evaluation of corn stover have shown that water hydrolysis prior to

  11. Overview of biomass pretreatment for cellulosic ethanol production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bioconversion of lignocellulosic biomass to ethanol is significantly hindered by the structural and chemical complexity of biomass, which makes these materials a challenge to be used as feedstocks for cellulosic ethanol production. Cellulose and hemicellulose, when hydrolyzed into their component su...

  12. NMR Studies of Biomass and its Reaction Products

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biomass refers to biological material derived from living or recently living organisms, such as wood, agricultural products and wastes, and alcohol fuels. An increasingly popular R&D approach is to convert biomass into industrial polymers or chemicals. NMR is an excellent technique for the character...

  13. Productivity and biomass of trematode (Digenea) parasites in lake ecosystems.

    PubMed

    Yurlova, N I

    2016-01-01

    The first estimation of the annual production and biomass of cercariae (free swimming transmission stage of digenetic trematodes) in a lake ecosystem has been performed. The biomass of cercariae is comparable with that of free-living invertebrates and may make a significant contribution to the energy flow in lake ecosystems. PMID:27021366

  14. Direct production of fractionated and upgraded hydrocarbon fuels from biomass

    SciTech Connect

    Felix, Larry G.; Linck, Martin B.; Marker, Terry L.; Roberts, Michael J.

    2014-08-26

    Multistage processing of biomass to produce at least two separate fungible fuel streams, one dominated by gasoline boiling-point range liquids and the other by diesel boiling-point range liquids. The processing involves hydrotreating the biomass to produce a hydrotreatment product including a deoxygenated hydrocarbon product of gasoline and diesel boiling materials, followed by separating each of the gasoline and diesel boiling materials from the hydrotreatment product and each other.

  15. Management and fertility control ecosystem carbon allocation to biomass production

    NASA Astrophysics Data System (ADS)

    Campioli, Matteo; Vicca, Sara; Janssens, Ivan

    2015-04-01

    Carbon (C) allocation within the ecosystem is one of the least understood processes in plant- and geo-sciences. The proportion of the C assimilated through photosynthesis (gross primary production, GPP) that is used for biomass production (BP) is a key variable of the C allocation process and it has been termed as biomass production efficiency (BPE). We investigated the potential drivers of BPE using a global dataset of BP, GPP, BPE and ancillary ecosystem characteristics (vegetation properties, climatic and environmental variables, anthropogenic impacts) for 131 sites comprising six major ecosystem types: forests, grasslands, croplands, tundra, boreal peatlands and marshes. We obtained two major findings. First, site fertility is the key driver of BPE across forests, with nutrient-rich forests allocating 58% of their photosynthates to BP, whereas this fraction is only 42% for nutrient-poor forests. Second, by disentangling the effect of management from the effect of fertility and by integrating all ecosystem types, we observed that BPE is globally not driven by the 'natural' site fertility, but by the positive effect brought by management on the nutrient availability. This resulted in managed ecosystems having substantially larger BPE than natural ecosystems. These findings will crucially improve our elucidation of the human impact on ecosystem functioning and our predictions of the global C cycle.

  16. Biomass Production System (BPS) plant growth unit.

    PubMed

    Morrow, R C; Crabb, T M

    2000-01-01

    The Biomass Production System (BPS) was developed under the Small Business Innovative Research (SBIR) program to meet science, biotechnology and commercial plant growth needs in the Space Station era. The BPS is equivalent in size to a double middeck locker, but uses its own custom enclosure with a slide out structure to which internal components mount. The BPS contains four internal growth chambers, each with a growing volume of more than 4 liters. Each of the growth chambers has active nutrient delivery, and independent control of temperature, humidity, lighting, and CO2 set-points. Temperature control is achieved using a thermoelectric heat exchanger system. Humidity control is achieved using a heat exchanger with a porous interface which can both humidify and dehumidify. The control software utilizes fuzzy logic for nonlinear, coupled temperature and humidity control. The fluorescent lighting system can be dimmed to provide a range of light levels. CO2 levels are controlled by injecting pure CO2 to the system based on input from an infrared gas analyzer. The unit currently does not scrub CO2, but has been designed to accept scrubber cartridges. In addition to providing environmental control, a number of features are included to facilitate science. The BPS chambers are sealed to allow CO2 and water vapor exchange measurements. The plant chambers can be removed to allow manipulation or sampling of specimens, and each chamber has gas/fluid sample ports. A video camera is provided for each chamber, and frame-grabs and complete environmental data for all science and hardware system sensors are stored on an internal hard drive. Data files can also be transferred to 3.5-inch disks using the front panel disk drive. PMID:11543164

  17. Siting Evaluation for Biomass-Ethanol Production in Hawaii

    SciTech Connect

    Kinoshita, C.M.; Zhou, J.

    2000-10-15

    This report examines four Hawaiian islands, Oahu, Hawaii, Maui, and Kauai, to identify three best combinations of potential sites and crops for producing dedicated supplies of biomass for conversion to ethanol. Key technical and economic factors considered in the siting evaluation include land availability (zoning and use), land suitability (agronomic conditions), potential quantities and costs of producing biomass feedstocks, infrastructure (including water and power supplies), transportation, and potential bioresidues to supplement dedicated energy crops.

  18. Distributed generation from biomass resources: Emerging potential for utilities

    SciTech Connect

    Whittier, J.; Haase, S.; Badger, P.C.

    1996-12-31

    Distributed generation (DG) offers potential to enhance the range of services provided by electric utilities. Competitive pressures experienced by the utility industry are sending simultaneous, and often conflicting, signals to planners concerned with busbar costs, market share and customer retention. DG technologies allow planners to address concurrent utility and customer concerns. DG will also open markets for additional commercial applications of diverse biomass technologies. Distributed generation offers multiple benefits both to utilities and to end users. Utilities may site new power production resources more readily and with lower capital costs and reduced financial risk than with larger power generation systems. Important benefits may accrue to the transmission and distribution (T&D) system including various forms of grid support (e.g., reduced line losses, voltage support, and power quality improvement), deferral of upgrades to substations, and provision of power in increments that match projected demand patterns. Other DG benefits may include assistance with customer waste disposal problems, fuel diversity, reduction in emissions of NO{sub x}, SO{sub x}, and CO{sub 2}, and increases in system reliability. Substantial changes in utility planning practices are required to accommodate DG. The utility must re-think planning procedures to begin from the customer and extend back to the system rather than beginning from comprehensive system planning at the power plant level. As competitive pressures encourage utilities to redefine business practices, DG may help to focus strategic responses to the market.

  19. Field performance of potential biomass feedstocks under no inputs in South Georgia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Warm-season perennial grasses have the greatest potential for biomass production in the Southeast for the emerging bioenergy industry. The larger root systems of perennial crops should be able to adapt to lower inputs of water and fertilizer, and should also contribute to soil carbon sequestration....

  20. Biomass burning and the production of methane. Draft report

    SciTech Connect

    Levine, J.S.; Cofer, W.R.; Pinto, J.P.

    1992-01-22

    Biomass burning and its environmental implications have also become important research elements of the International Geosphere-Biosphere Program and the International Global Atmospheric Chemistry Project. The production of atmospheric methane (CH4) by biomass burning will be assessed. The production of methane and other gaseous and particle carbon species resulting from biomass burning will be outlined. Field measurements and laboratory studies to quantify the emission ratio of methane and other carbon species will be reviewed. The historic database suggests that global biomass burning is increasing with time and is controlled by human activities. Present estimates indicate that biomass burning contributes between about 20 to about 60 Teragrams per year of carbon in the form of methane to the atmosphere. This represents only 5 to 15% of the global annual emissions of methane. Measurements do indicate that biomass burning is the overwhelming source of CH4 in tropical Africa. However, if the rate of global biomass burning increases at the rate that it has been over the last few decades, then the production of methane from biomass burning may become much more important on a global scale in the future.

  1. Method for creating high carbon content products from biomass oil

    DOEpatents

    Parker, Reginald; Seames, Wayne

    2012-12-18

    In a method for producing high carbon content products from biomass, a biomass oil is added to a cracking reactor vessel. The biomass oil is heated to a temperature ranging from about 100.degree. C. to about 800.degree. C. at a pressure ranging from about vacuum conditions to about 20,700 kPa for a time sufficient to crack the biomass oil. Tar is separated from the cracked biomass oil. The tar is heated to a temperature ranging from about 200.degree. C. to about 1500.degree. C. at a pressure ranging from about vacuum conditions to about 20,700 kPa for a time sufficient to reduce the tar to a high carbon content product containing at least about 50% carbon by weight.

  2. Biomass energy production. Citations from the International Aerospace Abstracts data base

    NASA Technical Reports Server (NTRS)

    Moore, P. W.

    1980-01-01

    These 210 citations from the international literature describe the production and/or utilization of most forms of biomass as a source of energy, fuel, food, and chemical intermediates or feedstocks. Biomass conversion by incineration, gasification, pyrolysis, hydrolysis, anaerobic digestion, or fermentation, as well as by catalytic, photosynthetic, chemosynthetic, and bio-electrochemical means are among the conversion processes considered. Discussions include biomass plantation and material productivity, transportation and equipment requirements, effects, comparisons of means and efficiencies of utilization and conversion, assessments of limitations, and evaluations of economic potential.

  3. Methane production from global biomass burning

    SciTech Connect

    Wei Min Hao; Ward, D.E.

    1993-11-20

    Emissions of methane from various sources of biomass burning are determined quantitatively for tropical, temperate, and boreal regions. About 85% of the total CH{sub 4} is emitted in the tropical area, which is mainly the result of shifting cultivation, fuelwood use, and deforestation. Methane emissions from biomass burning may have increased by at least 9% during the last decade because of increases in tropical deforestation and the use of fuelwood. Changes in land use practices and population growth in the tropics are possible causes of the increase of atmospheric CH{sub 4} concentration. 31 refs., 1 fig., 4 tabs.

  4. Biomass production from Trichoderma viride in nonconventional oat medium.

    PubMed

    Motta, F L; Santana, M H A

    2012-01-01

    Oatmeal, an alternative, renewable, and low-cost substrate, was used for the production of Trichoderma viride spores by submerged fermentation. The nonconventional oat medium was only supplemented with potato peptone, which is a green source of nitrogen for the microorganism. Because particles are suspended in the nonconventional oat medium, the characterization was based on viscosity, average particle diameter, size distribution, and porosity of the particles. Because of the complexity of the fungal biomass extraction, the dry weight and protein content were used as methods for quantifying the growth of T. viride. The inversion between the proportion of mycelia and spores was captured in the microscopic image analysis during the fermentation process. After 60 h, spores began to appear, accounting for most of the form present at 120 h of fermentation. The decrease in pH and the increase in glucose concentration during fermentation indicate that glucan hydrolysis occurs and that glucose is released into the medium. The potential for industrial applications of submerged fermentation with oats for biomass production of T. viride is noted in the results. This simple and easily controllable process has several advantages, including the use of low-cost substrates for the propagation of a microorganism that is widely used in scientific and commercial settings. PMID:22736524

  5. Sustainable Production of Switchgrass for Biomass Energy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Switchgrass (Panicum virgatum L.) is a C4 grass native to the North American tallgrass prairies, which historically extended from Mexico to Canada. It is the model perennial warm-season grass for biomass energy. USDA-ARS in Lincoln, NE has studied switchgrass continuously since 1936. Plot-scale rese...

  6. Development of Sustainable Landscape Designs for Improved Biomass Production in the U.S. Corn Belt

    NASA Astrophysics Data System (ADS)

    Bonner, Ian J.

    Demand for renewable and sustainable energy options has resulted in a significant commitment by the US Government to research pathways for fuel production from biomass. The research presented in this thesis describes one potential pathway to increase the amount of biomass available for biofuel production by integrating dedicated energy crops into agricultural fields. In the first chapter an innovative landscape design method based on subfield placement of an energy crop into row crop fields in central Iowa is used to reduce financial loss for farmers, increase and diversify biomass production, and improve soil resources. The second chapter explores how subfield management decisions may be made using high fidelity data and modeling to balance concerns of primary crop production and economics. This work provides critical forward looking support to agricultural land managers and stakeholders in the biomass and bioenergy industry for pathways to improving land stewardship and energy security.

  7. Biomass and energy productivity of Leucaena under humid subtropical conditions

    SciTech Connect

    Othman, A.B.; Prine, G.M.

    1984-01-01

    A table shows the amount and energy content of above-ground biomass produced in 1982 and 1983 by the 12 most productive of 62 accessions of Leucanena spp. established in 1979 at the University of Florida. Mean annual biomass production of the 12 accessions was 29.3 and 24.7 Mg/ha, with energy contents of 19,690 and 19,820 J/g, in 1982 and 1983 respectively.

  8. "Trojan Horse" strategy for deconstruction of biomass for biofuels production.

    SciTech Connect

    Sinclair, Michael B.; Hadi, Masood Z.; Timlin, Jerilyn Ann; Thomson, James; Whalen, Maureen; Thilmony, Roger; Tran-Gyamfi, Mary; Simmons, Blake Alexander; Sapra, Rajat

    2008-08-01

    Production of renewable biofuels to displace fossil fuels currently consumed in the transportation sector is a pressing multi-agency national priority. Currently, nearly all fuel ethanol is produced from corn-derived starch. Dedicated 'energy crops' and agricultural waste are preferred long-term solutions for renewable, cheap, and globally available biofuels as they avoid some of the market pressures and secondary greenhouse gas emission challenges currently facing corn ethanol. These sources of lignocellulosic biomass are converted to fermentable sugars using a variety of chemical and thermochemical pretreatments, which disrupt cellulose and lignin cross-links, allowing exogenously added recombinant microbial enzymes to more efficiently hydrolyze the cellulose for 'deconstruction' into glucose. This process is plagued with inefficiencies, primarily due to the recalcitrance of cellulosic biomass, mass transfer issues during deconstruction, and low activity of recombinant deconstruction enzymes. Costs are also high due to the requirement for enzymes and reagents, and energy-intensive and cumbersome pretreatment steps. One potential solution to these problems is found in synthetic biology; they propose to engineer plants that self-produce a suite of cellulase enzymes targeted to the apoplast for cleaving the linkages between lignin and cellulosic fibers; the genes encoding the degradation enzymes, also known as cellulases, are obtained from extremophilic organisms that grow at high temperatures (60-100 C) and acidic pH levels (<5). These enzymes will remain inactive during the life cycle of the plant but become active during hydrothermal pretreatment i.e., elevated temperatures. Deconstruction can be integrated into a one-step process, thereby increasing efficiency (cellulose-cellulase mass-transfer rates) and reducing costs. The proposed disruptive technologies address biomass deconstruction processes by developing transgenic plants encoding a suite of enzymes used

  9. Linking Gap Model with MODIS Biophysical Products for Biomass Estimation

    NASA Astrophysics Data System (ADS)

    Wang, D.; Sun, G.; Cai, Y.; Guo, Z.; Fu, A.; Ni, W.; Liu, D.

    With the development of earth observation technology and data processing technology biophysical data from remote sensing means such as MODIS LAI and NPP are accessible now However it is still difficult for direct measurement of biomass from remote sensors One possibility for overcoming this problem is using ecological models to link the vegetation parameters currently available from remote sensing to biomass In this paper a combined work is done for estimating forest biomass A calibrated gap model ZELIG was run to simulate the forest development in a temperate forested area in NE China The output relationship between age and biomass was linked to registered MODIS LAI NPP and land cover type images of the same area From the above work forest age or biomass was estimated from existing remote sensed data Obviously there is a lot of work to be done such as optimal combination of biophysical parameters to improve the linkage between MODIS product and ecological modeling

  10. Anaerobic bioassay of methane potential of microalgal biomass

    NASA Astrophysics Data System (ADS)

    Yen, Hong-Wei

    This study was undertaken to investigate the feasibility of using anaerobic digestion as a technique to recover solar energy embodied in excess algal biomass production harvested from Clemson University's high rate algal based Partitioned Aquaculture System (PAS) as an energy source to support PAS operations. In this study, four different organic substrates were loaded to anaerobic digesters in eight experimental trials, to ascertain the optimal combination of operational variables and effect of algal, or modified algal substrate upon methane production rate. The four substrates used in this study were: (1) a synthetic feedstock consisting of molasses and dog food, (2) a commercially obtained, readily degradable algal biomass (Spirulina ) in dry form, (3) PAS harvested and dewatered algal sludge, and (4) algal biomass blended with shredded waste paper or molasses as a carbon supplement for the adjustment of algal C/N ratio. Eight experimental trials using combinations of the four substrates were conducted in 15 liter digesters to investigate the effects of controlled digester parameters upon digester performance. Digesters operating at 20 days HRT, mesophilic digestion (35°C), and twice per day mixing at maximal loading rates produced maximal methane gas using PAS algal sludge. However, under these conditions overall methane production was less than 1000 ml CH4/l day. This low level of energy recovery from the fermentation of algal biomass (alone) is not energetically or economically favorable. Co-digestion of algal sludge and waste paper was investigated as a way to increase methane production. The data obtained from these trials suggest an optimum C/N ratio for co-digestion of algal sludge and waste paper in the range of 20--25/l. A balanced C/N ratio along with the stimulated increase in cellulase activity is suggested as likely reasons for increased methane production seen in co-digestion of algal sludge and waste paper. Yeast extract addition to anaerobic

  11. Biomass and lipid production of a local isolate Chlorella sorokiniana under mixotrophic growth conditions.

    PubMed

    Juntila, D J; Bautista, M A; Monotilla, W

    2015-09-01

    A local Chlorella sp. isolate with 97% rbcL sequence identity to Chlorella sorokiniana was evaluated in terms of its biomass and lipid production under mixotrophic growth conditions. Glucose-supplemented cultures exhibited increasing growth rate and biomass yield with increasing glucose concentration. Highest growth rate and biomass yield of 1.602 day(-1) and 687.5 mg L(-1), respectively, were achieved under 2 g L(-1) glucose. Nitrogen starvation up to 75% in the 1.0 g L(-1) glucose-supplemented culture was done to induce lipid accumulation and did not significantly affect the growth. Lipid content ranges from 20% to 27% dry weight. Nile Red staining showed more prominent neutral lipid bodies in starved mixotrophic cultures. C. sorokiniana exhibited enhanced biomass production under mixotrophy and more prominent neutral lipid accumulation under nitrogen starvation with no significant decrease in growth; hence, this isolate could be further studied to establish its potential for biodiesel production. PMID:25847795

  12. Potential Occupational Exposures and Health Risks Associated with Biomass-Based Power Generation

    PubMed Central

    Rohr, Annette C.; Campleman, Sharan L.; Long, Christopher M.; Peterson, Michael K.; Weatherstone, Susan; Quick, Will; Lewis, Ari

    2015-01-01

    Biomass is increasingly being used for power generation; however, assessment of potential occupational health and safety (OH&S) concerns related to usage of biomass fuels in combustion-based generation remains limited. We reviewed the available literature on known and potential OH&S issues associated with biomass-based fuel usage for electricity generation at the utility scale. We considered three potential exposure scenarios—pre-combustion exposure to material associated with the fuel, exposure to combustion products, and post-combustion exposure to ash and residues. Testing of dust, fungal and bacterial levels at two power stations was also undertaken. Results indicated that dust concentrations within biomass plants can be extremely variable, with peak levels in some areas exceeding occupational exposure limits for wood dust and general inhalable dust. Fungal spore types, identified as common environmental species, were higher than in outdoor air. Our review suggests that pre-combustion risks, including bioaerosols and biogenic organics, should be considered further. Combustion and post-combustion risks appear similar to current fossil-based combustion. In light of limited available information, additional studies at power plants utilizing a variety of technologies and biomass fuels are recommended. PMID:26206568

  13. Potential Occupational Exposures and Health Risks Associated with Biomass-Based Power Generation.

    PubMed

    Rohr, Annette C; Campleman, Sharan L; Long, Christopher M; Peterson, Michael K; Weatherstone, Susan; Quick, Will; Lewis, Ari

    2015-07-01

    Biomass is increasingly being used for power generation; however, assessment of potential occupational health and safety (OH&S) concerns related to usage of biomass fuels in combustion-based generation remains limited. We reviewed the available literature on known and potential OH&S issues associated with biomass-based fuel usage for electricity generation at the utility scale. We considered three potential exposure scenarios--pre-combustion exposure to material associated with the fuel, exposure to combustion products, and post-combustion exposure to ash and residues. Testing of dust, fungal and bacterial levels at two power stations was also undertaken. Results indicated that dust concentrations within biomass plants can be extremely variable, with peak levels in some areas exceeding occupational exposure limits for wood dust and general inhalable dust. Fungal spore types, identified as common environmental species, were higher than in outdoor air. Our review suggests that pre-combustion risks, including bioaerosols and biogenic organics, should be considered further. Combustion and post-combustion risks appear similar to current fossil-based combustion. In light of limited available information, additional studies at power plants utilizing a variety of technologies and biomass fuels are recommended. PMID:26206568

  14. Biomass production chamber air analysis of wheat study (BWT931)

    NASA Technical Reports Server (NTRS)

    Batten, J. H.; Peterson, B. V.; Berdis, E.; Wheeler, E. M.

    1993-01-01

    NASA's Controlled Ecological Life Support System (CELSS) biomass production chamber at John F. Kennedy Space Center provides a test bed for bioregenerative studies using plants to provide food, oxygen, carbon dioxide removal, and potable water to humans during long term space travel. Growing plants in enclosed environments has brought about concerns regarding the level of volatile organic compounds (VOC's) emitted from plants and the construction materials that make up the plant growth chambers. In such closed systems, the potential exists for some VOC's to reach toxic levels and lead to poor plant growth, plant death, or health problems for human inhabitants. This study characterized the air in an enclosed environment in which wheat cv. Yocora Rojo was grown. Ninty-four whole air samples were analyzed by gas chromatography/mass spectrometry throughout the eighty-four day planting. VOC emissions from plants and materials were characterized and quantified.

  15. Biomass Burning and the Production of Greenhouse Gases. Chapter 9

    NASA Technical Reports Server (NTRS)

    Levine, Joel S.

    1994-01-01

    Biomass burning is a source of greenhouse gases, carbon dioxide, methane, and nitrous oxide. In addition, biomass burning is a source of chemically active gases, including carbon monoxide, nonmethane hydrocarbons, and nitric oxide. These gases, along with methane, lead to the chemical production of tropospheric ozone (another greenhouse gas) as well as control the concentration of the hydroxyl radical, which regulates the lifetime of almost every atmospheric gas. Following biomass burning, biogenic emissions of nitrous oxide, nitric oxide, and methane are significantly enhanced. It is hypothesized that enhanced postburn biogenic emissions of these gases are related to fire-induced changes in soil chemistry and/or microbial ecology. Biomass burning, once believed to be a tropical phenomenon, has been demonstrated by satellite imagery to also be a regular feature of the world's boreal forests. One example of biomass burning is the extensive 1987 fire that destroyed more than 12 million acres of boreal forest in the People's Republic of China and across its border in the Soviet Union. Recent estimates indicate that almost all biomass burning is human-initiated and that it is increasing with time. With the formation of greenhouse and chemically active gases as direct combustion products and a longer-term enhancement of biogenic emissions of gases, biomass burning may be a significant driver for global change.

  16. Engineering analysis of biomass gasifier product gas cleaning technology

    SciTech Connect

    Baker, E.G.; Brown, M.D.; Moore, R.H.; Mudge, L.K.; Elliott, D.C.

    1986-08-01

    For biomass gasification to make a significant contribution to the energy picture in the next decade, emphasis must be placed on the generation of clean, pollutant-free gas products. This reports attempts to quantify levels of particulated, tars, oils, and various other pollutants generated by biomass gasifiers of all types. End uses for biomass gases and appropriate gas cleaning technologies are examined. Complete systems analysis is used to predit the performance of various gasifier/gas cleanup/end use combinations. Further research needs are identified. 128 refs., 20 figs., 19 tabs.

  17. Sustainability: The capacity of smokeless biomass pyrolysis for energy production, global carbon capture and sequestration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Application of modern smokeless biomass pyrolysis for biochar and biofuel production is potentially a revolutionary approach for global carbon capture and sequestration at gigatons of carbon (GtC) scales. A conversion of about 7% of the annual terrestrial gross photosynthetic product (120 GtC y-1) i...

  18. Fuels production by photoelectrolysis of water and photooxidation of soluble biomass materials

    SciTech Connect

    Sammells, A.F.; St. John, M.R.

    1984-03-20

    A process and apparatus for production of fuels by photoelectrolysis of water and photooxidation of water soluble biomass and a process for preparation of Schottky-type metalized, appropriately doped n-type semiconductor photochemical diodes suitable for use in the above process and apparatus. The production of hydrogen by photoelectrolysis of water as the cathodic reaction of an optically illuminated photochemical diode is effected in an aqueous electrolyte which comprises a biomass product which may be monosaccharides, polysaccharides, lignins, their partially oxidized products, and mixtures thereof which are oxidized as the anodic reaction of the photochemical diode producing liquid fuels and useful chemicals. Molecular oxygen evolution is avoided and utilization of biomass product provides a cost effective material to increase fuels and useful chemical production by photoelectrolysis of water at potentials substantially less than required for normal water electrolysis involving oxygen evolution.

  19. Reclamation of coppice forests in order to increase the potential of woody biomass in Serbia

    NASA Astrophysics Data System (ADS)

    Bjelanovic, I.; Krstic, M.

    2012-04-01

    Biomass makes 63% of the total renewable energy potential of Serbia. Here, the biomass from forests together with wood processing industry waste represent the second most important renewable source for energy production. The Action Plan for Biomass of Serbia (2010) shows that the technically exploitable biomass in the Republic of Serbia amounts annually 2.7 Mtoe. Here, the woody biomass (fuelwood, forest residue, wood processing industry residue, wood from trees outside the forest) accounts for 1.0 Mtoe while the rest originates from agricultural sources. According to the national forest inventory (2008), forest cover in Serbia accounts for 29% of the country area, having standing volume of 362.5 mil. m3 and annual increment of 9.1 mil. m3. More than half is state-owned and the rest 47% is in the private ownership. Coppice forests dominate in the forest stock (65%). According to Glavonjić (2010), northeastern and southwestern Serbia are the regions with greatest spatial forest distribution. The general forest condition is characterised by insufficient production volume, unsatisfactory stock density and forest cover, high percentage of degraded forests, unfavorable age structure, unfavorable health condition and weeded areas. Herewith, the basic measures for the improvement of forest fund (Forestry Development Strategy for Serbia, 2006) represent conversion of coppice forests, increase of forest cover and productivity of forest ecosystems by the ecologically, economically and socially acceptable methods. The actions include reclamation of degraded forests, re- and afforestation activities on abandoned agricultural, degraded and other treeless lands. The average standing volume of high forests is 254 m3·ha-1 with an annual increment of 5.5 m3·ha-1. On the contrary, coppice forests dispose 124 m3·ha-1 of standing volume, having an annual increment of 3.1 m3·ha-1. Here, estimated losses from coppice forests amount up to 3.5 mil. m3 wood annually. These data

  20. Glucanocellulosic ethanol: the undiscovered biofuel potential in energy crops and marine biomass.

    PubMed

    Falter, Christian; Zwikowics, Claudia; Eggert, Dennis; Blümke, Antje; Naumann, Marcel; Wolff, Kerstin; Ellinger, Dorothea; Reimer, Rudolph; Voigt, Christian A

    2015-01-01

    Converting biomass to biofuels is a key strategy in substituting fossil fuels to mitigate climate change. Conventional strategies to convert lignocellulosic biomass to ethanol address the fermentation of cellulose-derived glucose. Here we used super-resolution fluorescence microscopy to uncover the nanoscale structure of cell walls in the energy crops maize and Miscanthus where the typical polymer cellulose forms an unconventional layered architecture with the atypical (1, 3)-β-glucan polymer callose. This raised the question about an unused potential of (1, 3)-β-glucan in the fermentation of lignocellulosic biomass. Engineering biomass conversion for optimized (1, 3)-β-glucan utilization, we increased the ethanol yield from both energy crops. The generation of transgenic Miscanthus lines with an elevated (1, 3)-β-glucan content further increased ethanol yield providing a new strategy in energy crop breeding. Applying the (1, 3)-β-glucan-optimized conversion method on marine biomass from brown macroalgae with a naturally high (1, 3)-β-glucan content, we not only substantially increased ethanol yield but also demonstrated an effective co-fermentation of plant and marine biomass. This opens new perspectives in combining different kinds of feedstock for sustainable and efficient biofuel production, especially in coastal regions. PMID:26324382

  1. Glucanocellulosic ethanol: the undiscovered biofuel potential in energy crops and marine biomass

    PubMed Central

    Falter, Christian; Zwikowics, Claudia; Eggert, Dennis; Blümke, Antje; Naumann, Marcel; Wolff, Kerstin; Ellinger, Dorothea; Reimer, Rudolph; Voigt, Christian A.

    2015-01-01

    Converting biomass to biofuels is a key strategy in substituting fossil fuels to mitigate climate change. Conventional strategies to convert lignocellulosic biomass to ethanol address the fermentation of cellulose-derived glucose. Here we used super-resolution fluorescence microscopy to uncover the nanoscale structure of cell walls in the energy crops maize and Miscanthus where the typical polymer cellulose forms an unconventional layered architecture with the atypical (1, 3)-β-glucan polymer callose. This raised the question about an unused potential of (1, 3)-β-glucan in the fermentation of lignocellulosic biomass. Engineering biomass conversion for optimized (1, 3)-β-glucan utilization, we increased the ethanol yield from both energy crops. The generation of transgenic Miscanthus lines with an elevated (1, 3)-β-glucan content further increased ethanol yield providing a new strategy in energy crop breeding. Applying the (1, 3)-β-glucan-optimized conversion method on marine biomass from brown macroalgae with a naturally high (1, 3)-β-glucan content, we not only substantially increased ethanol yield but also demonstrated an effective co-fermentation of plant and marine biomass. This opens new perspectives in combining different kinds of feedstock for sustainable and efficient biofuel production, especially in coastal regions. PMID:26324382

  2. [Bio-oil production from biomass pyrolysis in molten salt].

    PubMed

    Ji, Dengxiang; Cai, Tengyue; Ai, Ning; Yu, Fengwen; Jiang, Hongtao; Ji, Jianbing

    2011-03-01

    In order to investigate the effects of pyrolysis conditions on bio-oil production from biomass in molten salt, experiments of biomass pyrolysis were carried out in a self-designed reactor in which the molten salt ZnCl2-KCl (with mole ratio 7/6) was selected as heat carrier, catalyst and dispersion agent. The effects of metal salt added into ZnCl2-KCl and biomass material on biomass pyrolysis were discussed, and the main compositions of bio-oil were determined by GC-MS. Metal salt added into molten salt could affect pyrolysis production yields remarkably. Lanthanon salt could enhance bio-oil yield and decrease water content in bio-oil, when mole fraction of 5.0% LaCl3 was added, bio-oil yield could reach up to 32.0%, and water content of bio-oil could reduce to 61.5%. The bio-oil and char yields were higher when rice straw was pyrolysed, while gas yield was higher when rice husk was used. Metal salts showed great selectivity on compositions of bio-oil. LiCl and FeCl2 promoted biomass to pyrolyse into smaller molecular weight compounds. CrCl3, CaCl2 and LaCl3 could restrain second pyrolysis of bio-oil. The research provided a scientific reference for production of bio-oil from biomass pyrolysis in molten salt. PMID:21650030

  3. Maintaining environmental quality while expanding biomass production: Sub-regional U.S. policy simulations

    SciTech Connect

    Egbendewe-Mondzozo, Aklesso; Swinton, S.; Izaurralde, Roberto C.; Manowitz, David H.; Zhang, Xuesong

    2013-03-01

    This paper evaluates environmental policy effects on ligno-cellulosic biomass production and environ- mental outcomes using an integrated bioeconomic optimization model. The environmental policy integrated climate (EPIC) model is used to simulate crop yields and environmental indicators in current and future potential bioenergy cropping systems based on weather, topographic and soil data. The crop yield and environmental outcome parameters from EPIC are combined with biomass transport costs and economic parameters in a representative farmer profit-maximizing mathematical optimization model. The model is used to predict the impact of alternative policies on biomass production and environmental outcomes. We find that without environmental policy, rising biomass prices initially trigger production of annual crop residues, resulting in increased greenhouse gas emissions, soil erosion, and nutrient losses to surface and ground water. At higher biomass prices, perennial bioenergy crops replace annual crop residues as biomass sources, resulting in lower environmental impacts. Simulations of three environmental policies namely a carbon price, a no-till area subsidy, and a fertilizer tax reveal that only the carbon price policy systematically mitigates environmental impacts. The fertilizer tax is ineffectual and too costly to farmers. The no-till subsidy is effective only at low biomass prices and is too costly to government.

  4. Rationally engineered synthetic coculture for improved biomass and product formation.

    PubMed

    Santala, Suvi; Karp, Matti; Santala, Ville

    2014-01-01

    In microbial ecosystems, bacteria are dependent on dynamic interspecific interactions related to carbon and energy flow. Substrates and end-metabolites are rapidly converted to other compounds, which protects the community from high concentrations of inhibitory molecules. In biotechnological applications, pure cultures are preferred because of the more straight-forward metabolic engineering and bioprocess control. However, the accumulation of unwanted side products can limit the cell growth and process efficiency. In this study, a rationally engineered coculture with a carbon channeling system was constructed using two well-characterized model strains Escherichia coli K12 and Acinetobacter baylyi ADP1. The directed carbon flow resulted in efficient acetate removal, and the coculture showed symbiotic nature in terms of substrate utilization and growth. Recombinant protein production was used as a proof-of-principle example to demonstrate the coculture utility and the effects on product formation. As a result, the biomass and recombinant protein titers of E. coli were enhanced in both minimal and rich medium simple batch cocultures. Finally, harnessing both the strains to the production resulted in enhanced recombinant protein titers. The study demonstrates the potential of rationally engineered cocultures for synthetic biology applications. PMID:25470793

  5. Macrobenthic biomass and production in a heterogenic subarctic fjord after invasion by the red king crab

    NASA Astrophysics Data System (ADS)

    Fuhrmann, Mona M.; Pedersen, Torstein; Ramasco, Virginie; Nilssen, Einar M.

    2015-12-01

    We studied the macrobenthic fauna and their production potential in Porsangerfjord, Northern Norway, in relation to environmental gradients and the recent invasion by the predatory red king crab into the outer fjord. The study area is characterized by a distinct along-fjord temperature gradient, with the influence of warmer Atlantic water in the outer fjord and year-round bottom temperatures around zero in the inner fjord. Benthic organisms can play a crucial role in ecosystem energy flow. Despite this, our knowledge of factors regulating benthic secondary production in high latitude ecosystems is limited. Macrobenthic abundance, biomass (B), production (P) and production-to-biomass ratio (P/B) were estimated from grab samples collected in 2010. Annual P/B ratios were calculated using a multi-parameter artificial neural network (ANN) model by Brey (2012). The mean abundance, biomass, production and P/B were 4611 ind. m- 2 (95% CI = 3994, 5316), 65 g ww m- 2 (95% CI = 51, 82), 174 kJ m- 2 y- 1 (95% CI = 151, 201) and 1.02 y- 1, respectively. Benthic biomass and production in the fjord were dominated by polychaetes. Spatial variability in P/B and production was mainly driven by community structure and differences in environmental habitat conditions. The inner basins of the fjord were characterized by high total production (439 kJ m- 2 y- 1), attributable to high standing stock biomass and community structure, despite cold bottom temperatures. In the middle and outer fjord, smaller taxa with low individual body masses increased the P/B ratios, but they did not compensate for the low biomass, thereby resulting in lower total production in these areas. The low biomass and the sparseness of large taxa in the outer and middle fjord may already be a result of predation by the invasive red king crab resulting in an overall lower macrobenthic production regime.

  6. Compositional and Agronomic Evaluation of Sorghum Biomass as a Potential Feedstock for Renewable Fuels

    SciTech Connect

    Dahlberg, J.; Wolfrum, E.; Bean, B.; Rooney, W. L.

    2011-12-01

    One goal of the Biomass Research and Development Technical Advisory Committee was to replace 30% of current U.S. petroleum consumption with biofuels by 2030. This will take mixtures of various feedstocks; an annual biomass feedstock such as sorghum will play an important role in meeting this goal. Commercial forage sorghum samples collected from field trials grown in Bushland, TX in 2007 were evaluated for both agronomic and compositional traits. Biomass compositional analysis of the samples was performed at the National Renewable Energy Lab in Golden, CO following NREL Laboratory Analytical Procedures. Depending on the specific cultivar, several additional years of yield data for this location were considered in establishing agronomic potential. Results confirm that sorghum forages can produce high biomass yields over multiple years and varied growing conditions. In addition, the composition of sorghum shows significant variation, as would be expected for most crops. Using theoretical estimates for ethanol production, the sorghum commercial forages examined in this study could produce an average of 6147 L ha{sup -1} of renewable fuels. Given its genetic variability, a known genomic sequence, a robust seed industry, and biomass composition, sorghum will be an important annual feedstock to meet the alternative fuel production goals legislated by the US Energy Security Act of 2007.

  7. Biomass production of sugarcane on narrow-rows in Florida

    SciTech Connect

    Cayton, J.E.; Eiland, B.R.

    1981-01-01

    Sugarcane production for biomass was examined on three narrow-row patterns in Florida. Equipment and production methods were modified for planting, spraying and harvesting the narrow-row patterns. No large increases in yields of vigorous varieties were found when compared to those from conventional rows. Some increases were observed in varieties which have low stalk populations. 4 refs.

  8. Cob biomass production in the western corn belt

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn residue is viewed as an abundant, inexpensive source of biomass that can be removed from fields for ethanol production without deleterious production or environmental effects if proper management is used according to some recent publications. Other publications indicate that corn residue needs ...

  9. Membrane recovery of phenolic acid co-products from biomass

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The technology to convert lignocellulosic biomass to biofuels is progressing with parallel efforts to develop processes to recover valuable natural products and generate additional revenue from these associated co-products. The lignified components of plant tissues contain phenolic acid structures s...

  10. Acid-Catalyzed Algal Biomass Pretreatment for Integrated Lipid and Carbohydrate-Based Biofuels Production

    SciTech Connect

    Laurens, L. M. L.; Nagle, N.; Davis, R.; Sweeney, N.; Van Wychen, S.; Lowell, A.; Pienkos, P. T.

    2014-11-12

    One of the major challenges associated with algal biofuels production in a biorefinery-type setting is improving biomass utilization in its entirety, increasing the process energetic yields and providing economically viable and scalable co-product concepts. We demonstrate the effectiveness of a novel, integrated technology based on moderate temperatures and low pH to convert the carbohydrates in wet algal biomass to soluble sugars for fermentation, while making lipids more accessible for downstream extraction and leaving a protein-enriched fraction behind. We studied the effect of harvest timing on the conversion yields, using two algal strains; Chlorella and Scenedesmus, generating biomass with distinctive compositional ratios of protein, carbohydrate, and lipids. We found that the late harvest Scenedesmus biomass had the maximum theoretical biofuel potential at 143 gasoline gallon equivalent (GGE) combined fuel yield per dry ton biomass, followed by late harvest Chlorella at 128 GGE per ton. Our experimental data show a clear difference between the two strains, as Scenedesmus was more successfully converted in this process with a demonstrated 97 GGE per ton. Our measurements indicated a release of >90% of the available glucose in the hydrolysate liquors and an extraction and recovery of up to 97% of the fatty acids from wet biomass. Techno-economic analysis for the combined product yields indicates that this process exhibits the potential to improve per-gallon fuel costs by up to 33% compared to a lipids-only process for one strain, Scenedesmus, grown to the mid-point harvest condition.

  11. Application Problem of Biomass Combustion in Greenhouses for Crop Production

    NASA Astrophysics Data System (ADS)

    Kawamura, Atsuhiro; Akisawa, Atsushi; Kashiwagi, Takao

    It is consumed much energy in fossil fuels to production crops in greenhouses in Japan. And fl ue gas as CO2 fertilization is used for growing crops in modern greenhouses. If biomass as renewable energy can use for production vegetables in greenhouses, more than 800,000 kl of energy a year (in crude oil equivalent) will be saved. In this study, at fi rst, we made the biomass combustion equipment, and performed fundamental examination for various pellet fuel. We performed the examination that considered an application to a real greenhouse next. We considered biomass as both a source of energy and CO2 gas for greenhouses, and the following fi ndings were obtained: 1) Based on the standard of CO2 gas fertilization to greenhouses, it is diffi cult to apply biomass as a CO2 fertilizer, so that biomass should be applied to energy use only, at least for the time being. 2) Practical biomass energy machinery for economy, high reliability and greenhouses satisfying the conservatism that it is easy is necessary. 3) It is necessary to develop crop varieties and cultivation systems requiring less strict environmental control. 4) Disposal of combustion ash occurring abundantly, effective practical use is necessary.

  12. A Review on Biomass Torrefaction Process and Product Properties

    SciTech Connect

    Jaya Shankar Tumuluru; Shahab Sokhansanj; Christopher T. Wright; J. Richard Hess; Richard D. Boardman

    2011-08-01

    Biomass Torrefaction is gaining attention as an important preprocessing step to improve the quality of biomass in terms of physical properties and chemical composition. Torrefaction is a slow heating of biomass in an inert or reduced environment to a maximum temperature of approximately 300 C. Torrefaction can also be defined as a group of products resulting from the partially controlled and isothermal pyrolysis of biomass occurring in a temperature range of 200-280 C. Thus, the process can be called a mild pyrolysis as it occurs at the lower temperature range of the pyrolysis process. At the end of the torrefaction process, a solid uniform product with lower moisture content and higher energy content than raw biomass is produced. Most of the smoke-producing compounds and other volatiles are removed during torrefaction, which produces a final product that will have a lower mass but a higher heating value. The present review work looks into (a) torrefaction process and different products produced during the process and (b) solid torrefied material properties which include: (i) physical properties like moisture content, density, grindability, particle size distribution and particle surface area and pelletability; (ii) chemical properties like proximate and ultimate composition; and (iii) storage properties like off-gassing and spontaneous combustion.

  13. Enhancing biomass and ethanol production by increasing NADPH production in Synechocystis sp. PCC 6803.

    PubMed

    Choi, Yun-Nam; Park, Jong Moon

    2016-08-01

    This study demonstrates that increased NADPH production can improve biomass and ethanol production in cyanobacteria. We over-expressed the endogenous zwf gene, which encodes glucose-6-phosphate dehydrogenase of pentose phosphate pathway, in the model cyanobacterium Synechocystis sp. PCC 6803. zwf over-expression resulted in increased NADPH production, and promoted biomass production compared to the wild type in both autotrophic and mixotrophic conditions. Ethanol production pathway including NADPH-dependent alcohol dehydrogenase was also integrated with and without zwf over-expression. Excessive NADPH production by zwf over-expression could improve both biomass and ethanol production in the autotrophic conditions. PMID:26951740

  14. Biomass Potentials in Different Maintenance Scenarios of Satoyama Woodlands

    NASA Astrophysics Data System (ADS)

    Terada, T.

    2012-04-01

    Woodlands near human settlements often have long histories of providing people with fuelwood and other organic materials. In Japan, these woodlands are called satoyama. While satoyama woodlands were historically coppiced to provide an essential source of fuelwood, many have been developed into residential areas as a result of the introduction of fossil fuels beginning in the 1960's. Remaining satoyamas were simply abandoned due to the loss of economic value from fuelwood. This has resulted in a loss of other satoyama-related functions such as their ecological function. In response to the abandonment of satoyamas, thousands of volunteer groups have formed since the 1990's to restore satoyama woodlands. However, in spite of the importance of grassroots volunteers, their actual activities are limited in spatial extent due to shortages of manpower, time, and maintenance skill. This suggests that more substantial incentives are necessary, if maintenance of satoyama woodlands is to be extended. This study focused on an increased attention of biomass enegy utilization from satoyama trees as a promising incentive, and estimated biomass potentials in different maintenance scenarios of satoyama woodlands through a case study site in peri-urban Tokyo. This study set four maintenance scenarios; a) ground cover removal, b) light-thinning, c) intensive-thinning, and d) rotational coppicing. Based on the scenarios, the amount of biomass obtained, bioenergy generated, and carbon reduced were estimated respectively by the combination of conducting tree measurement and applying a long-term forest dynamics estimation model. Since there is tradeoff between CO2 reduction through woodenergy utilization and CO2 fixation by standing trees, these two variables were analyzed in tandem. The scenario that produces the most woody biomass was rotational coppicing, the maintenance scenario which also mimics historical management regimes. Despite the lowest potential of CO2 fixation by standing

  15. Potential biomass in deep-sea hydrothermal vent ecosystem

    NASA Astrophysics Data System (ADS)

    Nakamura, K.; Takai, K.

    2012-12-01

    Since the first discovery of black smoker vents hosting chemosynthetic macrofaunal communities (Spiess et al., 1980), submarine hydrothermal systems and associated biota have attracted interest of many researchers (e.g., Humphris et al., 1995; Van Dover, 2000; Wilcock et al., 2004). In the past couple of decades, particular attention has been paid to chemolithoautotrophic microorganisms that sustain the hydrothermal vent-endemic animal communities as the primary producer. This type of microorganisms obtains energy from inorganic substances (e.g., sulfur, hydrogen, and methane) derived from hydrothermal vent fluids, and is often considered as an important modern analogue to the early ecosystems of the Earth as well as the extraterrestrial life in other planets and moons (e.g., Jannasch and Mottl, 1985; Nealson et al., 2005; Takai et al., 2006). Even today, however, the size of this type of chemosynthetic deep-sea hydrothermal vent ecosystem is largely unknown. Here, we present geophysical and geochemical constraints on potential biomass in the deep-sea hydrothermal vent ecosystem. The estimation of the potential biomass in the deep-sea hydrothermal vent ecosystem is based on hydrothermal fluid flux calculated from heat flux (Elderfield and Schltz, 1996), maximum chemical energy available from metabolic reactions during mixing between hydrothermal vent fluids and seawater (McCollom, 2007), and maintenance energy requirements of the chemolithoautotrophic microorganisms (Hoehler, 2004). The result shows that the most of metabolic energy sustaining the deep-sea hydrothermal vent ecosystem is produced by oxidation reaction of reduced sulfur, although some parts of the energy are derived from hydrogenotrophic and methanotrophic reactions. The overall total of the potential biomass in deep-sea hydrothermal vent ecosystem is calculated to be much smaller than that in terrestrial ecosystems including terrestrial plants. The big difference in biomass between the

  16. Nonenzymatic sugar production from biomass using biomass-derived γ-valerolactone.

    PubMed

    Luterbacher, Jeremy S; Rand, Jacqueline M; Alonso, David Martin; Han, Jeehoon; Youngquist, J Tyler; Maravelias, Christos T; Pfleger, Brian F; Dumesic, James A

    2014-01-17

    Widespread production of biomass-derived fuels and chemicals will require cost-effective processes for breaking down cellulose and hemicellulose into their constituent sugars. Here, we report laboratory-scale production of soluble carbohydrates from corn stover, hardwood, and softwood at high yields (70 to 90%) in a solvent mixture of biomass-derived γ-valerolactone (GVL), water, and dilute acid (0.05 weight percent H2SO4). GVL promotes thermocatalytic saccharification through complete solubilization of the biomass, including the lignin fraction. The carbohydrates can be recovered and concentrated (up to 127 grams per liter) by extraction from GVL into an aqueous phase by addition of NaCl or liquid CO2. This strategy is well suited for catalytic upgrading to furans or fermentative upgrading to ethanol at high titers and near theoretical yield. We estimate through preliminary techno-economic modeling that the overall process could be cost-competitive for ethanol production, with biomass pretreatment followed by enzymatic hydrolysis. PMID:24436415

  17. Global and regional potential for bioenergy from agricultural and forestry residue biomass

    SciTech Connect

    Gregg, Jay S.; Smith, Steven J.

    2010-02-11

    As co-products, agricultural and forestry residues represent a potential low cost, low carbon, source for bioenergy. A method is developed method for estimating the maximum sustainable amount of energy potentially available from agricultural and forestry residues by converting crop production statistics into associated residue, while allocating some of this resource to remain on the field to mitigate erosion and maintain soil nutrients. Currently, we estimate that the world produces residue biomass that could be sustainably harvested and converted into over 50 EJ yr-1 of energy. The top three countries where this resource is estimated to be most abundant are currently net energy importers: China, the United States (US), and India. The global potential from residue biomass is estimated to increase to approximately 80-95 EJ yr-1 by mid- to late- century, depending on physical assumptions such as of future crop yields and the amount of residue sustainably harvestable. The future market for biomass residues was simulated using the Object-Oriented Energy, Climate, and Technology Systems Mini Climate Assessment Model (ObjECTS MiniCAM). Utilization of residue biomass as an energy source is projected for the next century under different climate policy scenarios. Total global use of residue biomass is estimated to increase to 70-100 EJ yr-1 by mid- to late- century in a central case, depending on the presence of a climate policy and the economics of harvesting, aggregating, and transporting residue. Much of this potential is in developing regions of the world, including China, Latin America, Southeast Asia, and India.

  18. PRODUCTION OF NEW BIOMASS/WASTE-CONTAINING SOLID FUELS

    SciTech Connect

    David J. Akers; Glenn A. Shirey; Zalman Zitron; Charles Q. Maney

    2001-04-20

    CQ Inc. and its team members (ALSTOM Power Inc., Bliss Industries, McFadden Machine Company, and industry advisors from coal-burning utilities, equipment manufacturers, and the pellet fuels industry) addressed the objectives of the Department of Energy and industry to produce economical, new solid fuels from coal, biomass, and waste materials that reduce emissions from coal-fired boilers. This project builds on the team's commercial experience in composite fuels for energy production. The electric utility industry is interested in the use of biomass and wastes as fuel to reduce both emissions and fuel costs. In addition to these benefits, utilities also recognize the business advantage of consuming the waste byproducts of customers both to retain customers and to improve the public image of the industry. Unfortunately, biomass and waste byproducts can be troublesome fuels because of low bulk density, high moisture content, variable composition, handling and feeding problems, and inadequate information about combustion and emissions characteristics. Current methods of co-firing biomass and wastes either use a separate fuel receiving, storage, and boiler feed system, or mass burn the biomass by simply mixing it with coal on the storage pile. For biomass or biomass-containing composite fuels to be extensively used in the U.S., especially in the steam market, a lower cost method of producing these fuels must be developed that includes both moisture reduction and pelletization or agglomeration for necessary fuel density and ease of handling. Further, this method of fuel production must be applicable to a variety of combinations of biomass, wastes, and coal; economically competitive with current fuels; and provide environmental benefits compared with coal. Notable accomplishments from the work performed in Phase I of this project include the development of three standard fuel formulations from mixtures of coal fines, biomass, and waste materials that can be used in

  19. Ethylene dynamics in the CELSS biomass production chamber

    NASA Technical Reports Server (NTRS)

    Rakow, Allen L.

    1994-01-01

    A material balance model for ethylene was developed and applied retrospectively to data obtained in the Biomass Production Chamber of CELSS in order to calculate true plant production rates of ethylene. Four crops were analyzed: wheat, lettuce, soybean, and potato. The model represents an effort to account for each and every source and sink for ethylene in the system. The major source of ethylene is the plant biomass and the major sink is leakage to the surroundings. The result, expressed in the units of ppd/day, were converted to nl of ethylene per gram of plant dry mass per hour and compare favorably with recent glasshouse to belljar experiments.

  20. Nitrous oxide production in soils and the ratio of the fungal to bacterial biomass

    NASA Astrophysics Data System (ADS)

    Blagodatskiy, S. A.; Avksent'ev, A. A.; Davydova, M. A.; Blagodatskaya, E. V.; Kurakov, A. V.

    2008-12-01

    The proportion between the fungal and bacterial biomass, the potential activity of denitrification, and the intensity of N2O production were determined in the soils (chernozem and soddy-podzolic) of secondary biocenoses formed upon the abandoning of agricultural lands. The substitution of meadow and forest vegetation for agrocenoses has led to an increase in the percentage of the fungal biomass in the upper soil horizons. The rate of the net N2O production after the soil moistening positively correlated with the content of nitrates. In the soddy-podzolic soil (pH 3.7-5.6), the rate of nitrous oxide production was higher than that in the chernozem (pH 6.1-6.8). The rate of N2O production was inversely proportional to the bacterial biomass in the soils.

  1. Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer

    DOE PAGESBeta

    Eckard, Anahita Dehkhoda; Muthukumarappan, Kasiviswanathan; Gibbons, William

    2012-01-01

    Although lignocellulosic materials have a good potential to substitute current feedstocks used for ethanol production, conversion of these materials to fermentable sugars is still not economical through enzymatic hydrolysis. High cost of cellulase has prompted research to explore techniques that can prevent from enzyme deactivation. Colloidal proteins of casein can form monolayers on hydrophobic surfaces that alleviate the de-activation of protein of interest. Scanning electron microscope (SEM), fourier transform infrared spectroscopy (FT-IR), capillary electrophoresis (CE), and Kjeldahl and BSA protein assays were used to investigate the unknown mechanism of action of induced cellulase activity during hydrolysis of casein-treated biomass. Adsorptionmore » of casein to biomass was observed with all of the analytical techniques used and varied depending on the pretreatment techniques of biomass. FT-IR analysis of amides I and II suggested that the substructure of protein from casein or skim milk were deformed at the time of contact with biomass. With no additive, the majority of one of the cellulase mono-component, 97.1 ± 1.1, was adsorbed to CS within 24 h, this adsorption was irreversible and increased by 2% after 72 h. However, biomass treatment with skim-milk and casein reduced the adsorption to 32.9% ± 6.0 and 82.8% ± 6.0, respectively.« less

  2. Market potential for biomass co-fire in the four corners region

    SciTech Connect

    Whittier, J.; Haase, S.; Lynch, D.L.

    1996-12-31

    Fire suppression and heavy logging have caused major ecological changes in the pine forests of the San Juan-Rio Grande National Forest. A demonstration and administrative study sale was offered to determine the potential for ecosystem restoration in the forest. The conditions of the sale required the removal of trees from designated units that are typically classified as either Products Other than Logs (POL) or non-merchantable timber in addition to sawtimber. There are approximately 3.7 GW (net) of coal-fired power production capacity within 100 miles of the SJNF. Co-firing of biofuels with fossil fuels has significant potential for utility-scale power production. However, the economics and technical feasibility of co-firing are site-specific, depending on issues such as the availability of biomass, plant layout, existing combustion system technology, and the current cost of coal. The results of the feasibility analysis suggest limited market potential for forestry residues in area power plants. High biomass costs, despite USFS subsidies, coupled with low coal costs and coal supplier issues hinder the prospects for biomass co-fire opportunities. environmental considerations associated with reduced emissions (SO{sub x}, NO{sub x}, CO{sub 2}) are not major issues because of low sulfur coal that is widely available and limited markets for NO{sub x} and CO{sub 2} reductions.

  3. [Production of Kluyveromices fragilis biomass in deproteinized milk whey].

    PubMed

    Chinappi, I; Sánchez Crispín, J A

    2000-01-01

    The milk whey from a mature cheese factory deproteinised by acid thermic coagulation (pH 4.5 and 90 degrees C), provides a good culture media for the production of Kluyveromices fragilis biomass. The optimal experimental conditions for the maximal production of biomass were established by using fermenters with different capacity and design. For lactose concentration of 15 g/l, pH 4.5, 30 degrees C and aireation between 0.25 and 1 VVM, the duplication time was below two hours and 98% of the lactose was consumed. The obtained yield in dried weight was between 36 and 49% (g biomass/g lactose). The biomass (without broken cell) contain 46% protein on dry base and showed an "in vitro" digestibility of 65%. The organic mass decreased 80% after 12 hour of fermentation. This process eliminates a polluting agent and simultaneously, produces a biomass that could have industrial use as a protein complement in feeds. PMID:11460792

  4. Characterization of Spirulina biomass for CELSS diet potential

    NASA Technical Reports Server (NTRS)

    Tadros, Mahasin G.

    1993-01-01

    Cyanobacteria, Spirulina maxima as a biogenerative photosynthetic and an edible alga for the space craft crew in a CELSS, was evaluated in an effort to increase the growth rate, biomass, yield, and chemical analysis in continuous cultures. The cell characteristics were determined for cultures maintained at steady state with respect to the substrate concentration. The productivity increased in experiments exposed to low light (30 uE m(exp -2)s(exp -1). Oxygen evolved and protein production were higher in cultures exposed to low light intensity. There was a relationship between nitrate concentration and the yield of the culture. Increasing the concentration of nitrate in the growth medium up to 20 mM was enough to produce a culture having the same chemical composition as that of complete medium. High light was inhibiting the yield of the culture. Increasing the concentration of phosphate beyond 1 mM did not improve the yield of the culture. Increasing the concentration of sodium chloride in the growth medium did not affect the growth of the alga up to 0.1 M but beyond that the culture started to be stressed. The response to stress appeared in high production of total carbohydrate on the expense of protein production. The oxygen production was also higher in cultures stressed with sodium chloride.

  5. Gas exchange in NASA's biomass production chamber - A preprototype closed human life support system

    NASA Technical Reports Server (NTRS)

    Corey, Kenneth A.; Wheeler, Raymond M.

    1992-01-01

    The unique capabilities of the NASA biomass production chamber for monitoring and evaluating gas exchange rates are examined. Special emphasis is given to results with wheat and soybeans. The potential of the chamber as a preprototype of a closed human life support system is considered.

  6. Low input production of biomass from perennial grasses in the Coastal Plain of Georgia, USA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Warm-season perennial grasses have the greatest potential for biomass production in the Southeast. The larger root systems of perennial crops should be able to adapt to lower inputs of water and fertilizer, and should also contribute to soil carbon sequestration. This study was initiated in fall 2...

  7. Radiation use efficiency, biomass production, and grain yield in two maize hybrids differing in drought tolerance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Drought tolerant (DT) maize (Zea mays L.) hybrids have potential to increase yield under drought conditions. However, little information is known about the physiological determinations of yield in DT hybrids. Our objective was to assess radiation use efficiency (RUE), biomass production, and yield ...

  8. Recovery of phenolic compounds from biomass during ethanol production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biomass to ethanol conversion represents an alternative liquid fuel technology that does not need to compete with food crops. Maintaining agricultural production of commodity crops such as corn and soybeans for the food supply and using agricultural waste or low input energy crops grown on marginal ...

  9. Native grasses for biomass production at high elevations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Recent policies such as the Energy Independence Security Act of 2007 (EISA) and the second U.S. Renewable Fuel Standards (RFS), have targeted biofuel production and domestic energy independence. Hence, considerable interest has focused on producing plant biomass for conversion into biofuels for the...

  10. Process technologies for production of fuel ethanol from lignocellulosic biomass

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Lignocellulosic biomass such as corn stover, wheat straw, rice straw, and switchgrass can serve as low-cost feedstock for production of fuel ethanol. These feedstocks contain complex carbohydrates (cellulose and hemicelluloses) which need to be converted to fermentable sugars and then these sugars b...

  11. High biomass sorghum production across tillage systems and nitrogen rates

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bioenergy production has traditionally focused on perennial crops; however, these crops require an establishment period before they can be utilized. High biomass sorghum (Sorghum bicolor L. Moench) grown as an annual crop can be used during this establishment period, but typical yields and nutrient...

  12. The Mississippi University Research Consortium for the Utilization of Biomass: Production of Alternative Fuels from Waste Biomass Initiative

    SciTech Connect

    Drs. Mark E. Zapp; Todd French; Lewis Brown; Clifford George; Rafael Hernandez; Marvin Salin; Drs. Huey-Min Hwang, Ken Lee, Yi Zhang; Maria Begonia; Drs. Clint Williford; Al Mikell; Drs. Robert Moore; Roger Hester .

    2009-03-31

    conversion. All three of these processes are of particular interest to states in the Southeastern US since the agricultural products produced in this region are highly variable in terms of actual crop, production quantity, and the ability of land areas to support a particular type of crop. This greatly differs from the Midwestern US where most of this region's agricultural land supports one to two primary crops, such as corn and soybean. Therefore, developing processes which are relatively flexible in terms of biomass feedstock is key to the southeastern region of the US if this area is going to be a 'player' in the developing biomass to chemicals arena. With regard to the fermentation of syngas, research was directed toward developing improved biocatalysts through organism discovery and optimization, improving ethanol/acetic acid separations, evaluating potential bacterial contaminants, and assessing the use of innovative fermentors that are better suited for supporting syngas fermentation. Acid hydrolysis research was directed toward improved conversion yields and rates, acid recovery using membranes, optimization of fermenting organisms, and hydrolyzate characterization with changing feedstocks. Additionally, a series of development efforts addressed novel separation techniques for the separation of key chemicals from fermentation activities. Biogas related research focused on key factors hindering the widespread use of digester technologies in non-traditional industries. The digestion of acetic acids and other fermentation wastewaters was studied and methods used to optimize the process were undertaken. Additionally, novel laboratory methods were designed along with improved methods of digester operation. A search for better performing digester consortia was initiated coupled with improved methods to initiate their activity within digester environments. The third activity of the consortium generally studied the production of 'other' chemicals from waste biomass materials

  13. Biomass recycling heat technology and energy products

    NASA Astrophysics Data System (ADS)

    Tabakaev, R. B.; Gergelizhiu, P. S.; Kazakov, A. V.; Zavorin, A. S.

    2014-10-01

    Relevance is determined by necessity of utilizing of local low-grade fuels by energy equpment. Most widespread Tomsk oblast (Russian Federation region) low-grade fuels are described and listed. Capability of utilizing is analysed. Mass balances of heat-technology conversion materials and derived products are described. As a result, recycling capability of low-grade fuels in briquette fuel is appraised.

  14. Biomass removal: Effect on soil nutrients and productivity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Interest in renewable alternatives to fossil energy has increased. There is also a growing awareness of the impact of greenhouse gas emission on global climate change. Crop biomass can be used to make liquid fuels like ethanol. These cellulosic materials are also potential feedstock for controlled c...

  15. LIQUID BIO-FUEL PRODUCTION FROM NON-FOOD BIOMASS VIA HIGH TEMPERATURE STEAM ELECTROLYSIS

    SciTech Connect

    G. L. Hawkes; J. E. O'Brien; M. G. McKellar

    2011-11-01

    Bio-Syntrolysis is a hybrid energy process that enables production of synthetic liquid fuels that are compatible with the existing conventional liquid transportation fuels infrastructure. Using biomass as a renewable carbon source, and supplemental hydrogen from high-temperature steam electrolysis (HTSE), bio-syntrolysis has the potential to provide a significant alternative petroleum source that could reduce US dependence on imported oil. Combining hydrogen from HTSE with CO from an oxygen-blown biomass gasifier yields syngas to be used as a feedstock for synthesis of liquid transportation fuels via a Fischer-Tropsch process. Conversion of syngas to liquid hydrocarbon fuels, using a biomass-based carbon source, expands the application of renewable energy beyond the grid to include transportation fuels. It can also contribute to grid stability associated with non-dispatchable power generation. The use of supplemental hydrogen from HTSE enables greater than 90% utilization of the biomass carbon content which is about 2.5 times higher than carbon utilization associated with traditional cellulosic ethanol production. If the electrical power source needed for HTSE is based on nuclear or renewable energy, the process is carbon neutral. INL has demonstrated improved biomass processing prior to gasification. Recyclable biomass in the form of crop residue or energy crops would serve as the feedstock for this process. A process model of syngas production using high temperature electrolysis and biomass gasification is presented. Process heat from the biomass gasifier is used to heat steam for the hydrogen production via the high temperature steam electrolysis process. Oxygen produced form the electrolysis process is used to control the oxidation rate in the oxygen-blown biomass gasifier. Based on the gasifier temperature, 94% to 95% of the carbon in the biomass becomes carbon monoxide in the syngas (carbon monoxide and hydrogen). Assuming the thermal efficiency of the power

  16. Biological production of liquid fuels from biomass

    SciTech Connect

    1982-01-01

    A scheme for the production of liquid fuels from renewable resources such as poplar wood and lignocellulosic wastes from a refuse hydropulper was investigated. The particular scheme being studied involves the conversion of a cellulosic residue, resulting from a solvent delignified lignocellulosic feed, into either high concentration sugar syrups or into ethyl and/or butyl alcohol. The construction of a pilot apparatus for solvent delignifying 150 g samples of lignocellulosic feeds was completed. Also, an analysis method for characterizing the delignified product has been selected and tested. This is a method recommended in the Forage Fiber Handbook. Delignified samples are now being prepared and tested for their extent of delignification and susceptibility to enzyme hydrolysis. Work is continuing on characterizing the cellulase and cellobiase enzyme systems derived from the YX strain of Thermomonospora.

  17. Photosynthetic pathway and biomass energy production.

    PubMed

    Marzola, D L; Bartholomew, D P

    1979-08-10

    The current interest in locating new or alternative sources of energy has focused attention on solar energy capture by crops that can be subsequently utilized as a substitute for fossil fuels. The very high productivity of sugarepane and the fact that it accumulates sugars that are directly fermentable to alcohol may have caused seemingly less productive crops to be overlooked. We show here that recoverable alcohol from achievable commercial yields of pineapple can actually equal that of sugarcane, with the pineapple crop requiring only a fraction of the water used by sugarcane. Pineapple is well adapted to the subhumid or semiarid tropics and thus is particularly well suited for exploiting large areas not now under cultivation with any crop of commercial value. PMID:17729660

  18. Improved conversion of herbaceous biomass to biofuels: Potential for modification of key plant characteristics

    SciTech Connect

    Sladden, S.E.; Bransby, D.I. . Dept. of Agronomy and Soils)

    1989-10-01

    Biomass crops are converted to fuels via biochemical and thermochemical processes. The process preferred depends on properties and cost of available feedstocks, and on the specific products desired. Since most mature biomass crops are composed of up to 80% cell wall fibers, the properties of these fibers determine, to a large degree, the conversion potential of the crop. However, biomass crops also contain small amounts of proteins, soluble carbohydrates and interfering materials (e.g., tannins and silica) which also influence the desirability of the feedstock in specific conversion processes. Fortunately, wide variation exists in the chemical composition of potential biomass crops. Although the chemical composition of feedstocks can be influenced significantly with judicious management has species selection, some traits are sufficiently heritable to permit breeding for improved feedstock composition. In addition to breeding for specific compositional traits directly, selection for in vitro digestibility or for easily-measured canopy or physiological traits may lead to more rapid and efficient progress in feedstock improvement, provided those measurements are highly-correlated with desirable feedstock composition. At the same time breeders must improve, or at least avoid damaging, stand longevity, tendency of plants to lodge, and establishment traits (e.g., disease resistance and seedling vigor). 46 refs., 8 tabs.

  19. Evaluating root zone water quality impacts associated with various biomass production systems across landscape positions

    NASA Astrophysics Data System (ADS)

    Welsh, W.; Zhou, X.; Helmers, M. J.; Schulte Moore, L.; Isenhart, T.; Kolka, R.

    2011-12-01

    Evaluating the water quality impacts of biomass production systems is essential to assessing biomass production systems' environmental impacts. The objective of this study is to determine potential water quality impacts of various production systems across different landscape positions. Five production systems are being evaluated: (1) continuous corn, (2) corn-soy/triticale-soy, (3) switchgrass, (4) triticale/sorghum, and (5) triticale/trees, at five landscape locations: (1) summit, (2) shoulder, (3) backslope, (4) toeslope, and (5) floodplain. Each production system is randomly assigned within three replicates at each landscape location. Soil water samples are taken monthly during the growing season from two suction lysimeters per plot at a depth of 60cm. Initial results indicate significant differences between the production systems and a likely association between fertilizer input and NO3-N concentrations with corn plots having the highest concentration and the tree plots having the lowest. Relatively high concentrations in the corn and sorghum plots following fertilization were observed the first year and similar results are being observed early in the second year of observations. A significant landscape effect was observed late in the growing season during the first year of this study. Quantifying the environmental impacts of biomass production systems will aid in optimizing deployment as producers gear up to meet biomass production demand.

  20. Assessment of the phytoextraction potential of high biomass crop plants.

    PubMed

    Hernández-Allica, Javier; Becerril, José M; Garbisu, Carlos

    2008-03-01

    A hydroponic screening method was used to identify high biomass crop plants with the ability to accumulate metals. Highest values of shoot accumulation were found in maize cv. Ranchero, rapeseed cv. Karat, and cardoon cv. Peralta for Pb (18 753 mg kg(-1)), Zn (10 916 mg kg(-1)), and Cd (242 mg kg(-1)), respectively. Subsequently, we tested the potential of these three cultivars for the phytoextraction of a metal spiked compost, finding out that, in cardoon and maize plants, increasing Zn and Cd concentrations led to lower values of root and shoot DW. By contrast, rapeseed shoot growth was not significantly affected by Cd concentration. Finally, a metal polluted soil was used to check these cultivars' phytoextraction capacity. Although the soil was phytotoxic enough to prevent the growth of cardoon and rapeseed plants, maize plants phytoextracted 3.7 mg Zn pot(-1). We concluded that the phytoextraction performance of cultivars varies depending on the screening method used. PMID:17644228

  1. Thermo-chemical and biological conversion potential of various biomass feedstocks to ethanol

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The goal of this study is to evaluate the potential and the economy of producing ethanol from gasification-fermentation of various biomass feedstocks. The biomass feedstocks include winter cover crops (wheat, rye, clover, hairy betch), summer cover crop (sunhemp), chicken litter, and woody biomass. ...

  2. Attached cultivation for improving the biomass productivity of Spirulina platensis.

    PubMed

    Zhang, Lanlan; Chen, Lin; Wang, Junfeng; Chen, Yu; Gao, Xin; Zhang, Zhaohui; Liu, Tianzhong

    2015-04-01

    To improve cultivation efficiency for microalgae Spirulina platensis is related to increase its potential use as food source and as an effective alternative for CO2 fixation. The present work attempted to establish a technique, namely attached cultivation, for S. platensis. Laboratory experiments were made firstly to investigate optimal conditions on attached cultivation. The optimal conditions were found: 25 g m(-2) for initial inoculum density using electrostatic flocking cloth as substrata, light intensity lower than 200 μmol m(-2) s(-1), CO2 enriched air flow (0.5%) at a superficial aeration rate of 0.0056 m s(-1) in a NaHCO3-free Zarrouk medium. An outdoor attached cultivation bench-scale bioreactor was built and a 10d culture of S. platensis was carried out with daily harvesting. A high footprint areal biomass productivity of 60 g m(-2) d(-1) was obtained. The nutrition of S. platensis with attached cultivation is identical to that with conventional liquid cultivation. PMID:25647023

  3. Advances in the production of sponge biomass Aplysina aerophoba--a model sponge for ex situ sponge biomass production.

    PubMed

    Hausmann, Rudolf; Vitello, Marco P; Leitermann, Frank; Syldatk, Christoph

    2006-06-25

    Sponges are a promising source of organic compounds of potential interest regarding industrial and medical applications. For detailed studies on such compounds, large amounts of sponge biomass are required. Obtaining that is at present extremely difficult because most sponges are relatively rare in nature and their mass cultivation in the laboratory has not yet been accomplished. In this study the possibility of culturing Aplysina aerophoba fragments in laboratory was examined. While a substantial biomass increase was not yet observed, we achieved fragmented sponge tissue to develop into a functional sponge as a first success. PMID:16697067

  4. Biomass and productivity of trematode parasites in pond ecosystems.

    PubMed

    Preston, Daniel L; Orlofske, Sarah A; Lambden, Jason P; Johnson, Pieter T J

    2013-05-01

    1. Ecologists often measure the biomass and productivity of organisms to understand the importance of populations and communities in the flow of energy through ecosystems. Despite the central role of such studies in the advancement of freshwater ecology, there has been little effort to incorporate parasites into studies of freshwater energy flow. This omission is particularly important considering the roles that parasites sometimes play in shaping community structure and ecosystem processes. 2. Using quantitative surveys and dissections of over 1600 aquatic invertebrate and amphibian hosts, we calculated the ecosystem-level biomass and productivity of trematode parasites alongside the biomass of free-living aquatic organisms in three freshwater ponds in California, USA. 3. Snails and amphibian larvae, which are both important intermediate trematode hosts, dominated the dry biomass of free-living organisms across ponds (snails = 3.2 g m(-2); amphibians = 3.1 g m(-2)). An average of 33.5% of mature snails were infected with one of six trematode taxa, amounting to a density of 13 infected snails m(-2) of pond substrate. Between 18% and 33% of the combined host and parasite biomass within each infected snail consisted of larval trematode tissue, which collectively accounted for 87% of the total trematode biomass within the three ponds. Mid-summer trematode dry biomass averaged 0.10 g m(-2), which was equal to or greater than that of the most abundant insect orders (coleoptera = 0.10 g m(-2), odonata = 0.08 g m(-2), hemiptera = 0.07 g m(-2) and ephemeroptera = 0.03 g m(-2)). 4. On average, each trematode taxon produced between 14 and 1660 free-swimming larvae (cercariae) infected snail(-1) 24 h(-1) in mid-summer. Given that infected snails release cercariae for 3-4 months a year, the pond trematode communities produced an average of 153 mg m(-2) yr(-1) of dry cercarial biomass (range = 70-220 mg m(-2) yr(-1)). 5. Our results suggest that a significant amount of energy

  5. Production of protein-rich fungal biomass in an airlift bioreactor using vinasse as substrate.

    PubMed

    Nitayavardhana, Saoharit; Issarapayup, Kerati; Pavasant, Prasert; Khanal, Samir Kumar

    2013-04-01

    The potential for large-scale production of an edible fungus, Rhizopus oligosporus, on a liquid residue from sugar-to-ethanol production, vinasse, was investigated. An airlift bioreactor (2.5-L working volume) was used for cultivating the fungus on 75% (v/v) vinasse with nutrient supplementation (nitrogen and phosphorus) at 37°C and pH 5.0. Aeration rates were varied from 0.5, 1.0, 1.5 to 2.0 volume(air)/volume(liquid)/min (vvm). The fungal biomass yield depended on the aeration rate, and the highest fungal biomass obtained was 8.04±0.80 (g(biomass increase)/g(initial biomass)) at 1.5vvm. The observed reductions in organic content by 80% (as soluble chemical oxygen demand) suggest the potential of recycling treated effluent as process water for in-plant use or for land applications. The fungal biomass contained ~50% crude protein and the essential amino acids contents were comparable to commercial protein sources for aquatic feeds (fishmeal and soybean meal), with the exception of methionine and phenylalanine. PMID:23434806

  6. Cyanobacteria cultivation in industrial wastewaters and biodiesel production from their biomass: a review.

    PubMed

    Balasubramanian, Lavanya; Subramanian, Geetha; Nazeer, Thayiba Thanveer; Simpson, Hannah Shalini; Rahuman, Shifina T; Raju, Preetha

    2011-01-01

    As an alternative fuel biodiesel has become increasingly important due to diminishing petroleum reserves and adverse environmental consequences of exhaust gases from petroleum-fueled engines. Recently, research interest has focused on the production of biofuel from microalgae. Cyanobacteria appeared to be suitable candidates for cultivation in wastes and wastewaters because they produce biomass in satisfactory quantity and can be harvested relatively easily due to their size and structure. In addition, their biomass composition can be manipulated by several environmental and operational factors to produce biomass with concrete characteristics. Herein, we review the culture of cyanobacteria in wastewaters and also the potential resources that can be transformed into biodiesel successfully for meeting the ever-increasing demand for biodiesel production. PMID:21838795

  7. Towards Predicting Basin-Wide Invertebrate Organic Biomass and Production in Marine Sediments from a Coastal Sea

    PubMed Central

    Burd, Brenda J.; Macdonald, Tara A.; van Roodselaar, Albert

    2012-01-01

    Detailed knowledge of environmental conditions is required to understand faunal production in coastal seas with topographic and hydrographic complexity. We test the hypothesis that organic biomass and production of subtidal sediment invertebrates throughout the Strait of Georgia, west coast of Canada, can be predicted by depth, substrate type and organic flux modified to reflect lability and age of material. A basin-wide database of biological, geochemical and flux data was analysed using an empirical production/biomass (P/B) model to test this hypothesis. This analysis is unique in the spatial extent and detail of P/B and concurrent environmental measurements over a temperate coastal region. Modified organic flux was the most important predictor of organic biomass and production. Depth and substrate type were secondary modifiers. Between 69–74% of variability in biomass and production could be explained by the combined environmental factors. Organisms <1 mm were important contributors to biomass and production primarily in shallow, sandy sediments, where high P/B values were found despite low organic flux. Low biomass, production, and P/B values were found in the deep, northern basin and mainland fjords, which had silty sediments, low organic flux, low biomass of organisms <1 mm, and dominance by large, slow-growing macrofauna. In the highest organic flux and biomass areas near the Fraser River discharge, production did not increase beyond moderate flux levels. Although highly productive, this area had low P/B. Clearly, food input is insufficient to explain the complex patterns in faunal production revealed here. Additional environmental factors (depth, substrate type and unmeasured factors) are important modifiers of these patterns. Potential reasons for the above patterns are explored, along with a discussion of unmeasured factors possibly responsible for unexplained (30%) variance in biomass and production. We now have the tools for basin-wide first

  8. Succinate production from CO2-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing

    PubMed Central

    Lee, Jungseok; Sim, Sang Jun; Bott, Michael; Um, Youngsoon; Oh, Min-Kyu; Woo, Han Min

    2014-01-01

    The potential for production of chemicals from microalgal biomass has been considered as an alternative route for CO2 mitigation and establishment of biorefineries. This study presents the development of consolidated bioprocessing for succinate production from microalgal biomass using engineered Corynebacterium glutamicum. Starch-degrading and succinate-producing C. glutamicum strains produced succinate (0.16 g succinate/g total carbon source) from a mixture of starch and glucose as a model microalgal biomass. Subsequently, the engineered C. glutamicum strains were able to produce succinate (0.28 g succinate/g of total sugars including starch) from pretreated microalgal biomass of CO2-grown Chlamydomonas reinhardtii. For the first time, this work shows succinate production from CO2 via sequential fermentations of CO2-grown microalgae and engineered C. glutamicum. Therefore, consolidated bioprocessing based on microalgal biomass could be useful to promote variety of biorefineries. PMID:25056811

  9. Considerations for Sustainable Biomass Production in Quercus-Dominated Forest Ecosystems

    NASA Astrophysics Data System (ADS)

    Bruckman, Viktor; Yan, Shuai; Hochbichler, Eduard

    2013-04-01

    Our current energy system is mainly based on carbon (C) intensive metabolisms, resulting in great effects on the earth's biosphere. The majority of the energy sources are fossil (crude oil, coal, natural gas) and release CO2 in the combustion (oxidation) process which takes place during utilization of the energy. C released to the atmosphere was once sequestered by biomass over a time span of millions of years and is now being released back into the atmosphere within a period of just decades. In the context of green and CO2 neutral Energy, there is an on-going debate regarding the potentials of obtaining biomass from forests on multiple scales, from stand to international levels. Especially in the context of energy, it is highlighted that biomass is an entirely CO2 neutral feedstock since the carbon stored in wood originates from the atmospheric CO2 pool and it was taken up during plant growth. It needs systems approaches in order to justify this statement and ensure sustainability covering the whole life-cycle from biomass production to (bio)energy consumption. There are a number of Quercus woodland management systems focussing solely on woody biomass production for energetic utilization or a combination with traditional forestry and high quality timber production for trades and industry. They have often developed regionally as a consequence of specific demands and local production capacities, which are mainly driven by environmental factors such as climate and soil properties. We assessed the nutritional status of a common Quercus-dominated forest ecosystem in northern Austria, where we compared biomass- with belowground C and nutrient pools in order to identify potential site limits if the management shifts towards systems with a higher level of nutrient extraction. Heterogeneity of soils, and soil processes are considered, as well as other, growth-limiting factors (e.g. precipitation) and species-specific metabolisms and element translocation.

  10. Sustainability of biofuels and renewable chemicals production from biomass.

    PubMed

    Kircher, Manfred

    2015-12-01

    In the sectors of biofuel and renewable chemicals the big feedstock demand asks, first, to expand the spectrum of carbon sources beyond primary biomass, second, to establish circular processing chains and, third, to prioritize product sectors exclusively depending on carbon: chemicals and heavy-duty fuels. Large-volume production lines will reduce greenhouse gas (GHG) emission significantly but also low-volume chemicals are indispensable in building 'low-carbon' industries. The foreseeable feedstock change initiates innovation, securing societal wealth in the industrialized world and creating employment in regions producing biomass. When raising the investments in rerouting to sustainable biofuel and chemicals today competitiveness with fossil-based fuel and chemicals is a strong issue. Many countries adopted comprehensive bioeconomy strategies to tackle this challenge. These public actions are mostly biased to biofuel but should give well-balanced attention to renewable chemicals as well. PMID:26256682

  11. Linking geophysics and soil function modelling - biomass production

    NASA Astrophysics Data System (ADS)

    Krüger, J.; Franko, U.; Werban, U.; Fank, J.

    2012-04-01

    The iSOIL project aims at reliable mapping of soil properties and soil functions with various methods including geophysical, spectroscopic and monitoring techniques. The general procedure contains three steps (i) geophysical monitoring, (ii) generation of soil property maps and (iii) process modelling. The objective of this work is to demonstrate the mentioned procedure with a focus on process modelling. It deals with the dynamics of soil water and the direct influence on crop biomass production. The new module PLUS extends CANDY to simulate crop biomass production based on environmental influences. A soil function modelling with an adapted model parameterisation based on data of ground penetration radar (GPR) and conductivity (EM38) was realized. This study shows an approach to handle heterogeneity of soil properties with geophysical data used for biomass production modelling. The Austrian field site Wagna is characterised by highly heterogenic soil with fluvioglacial gravel sediments. The variation of thickness of topsoil above a sandy subsoil with gravels strongly influences the soil water balance. EM38, mounted on a mobile platform, enables to rapidly scan large areas whereas GPR requires a greater logistical effort. However, GPR can detect exact soil horizon depth between topsoil and subsoil, the combination of both results in a detailed large scale soil map. The combined plot-specific GPR and field site EM38 measurements extends the soil input data and improves the model performance of CANDY PLUS for plant biomass production (Krüger et al. 2011). The example demonstrates how geophysics provides a surplus of data for agroecosystem modelling which identifies and contributes alternative options for agricultural management decisions. iSOIL - "Interactions between soil related sciences - Linking geophysics, soil science and digital soil mapping" is a Collaborative Project (Grant Agreement number 211386) co-funded by the Research DG of the European Commission

  12. Pectin-rich biomass as feedstock for fuel ethanol production.

    PubMed

    Edwards, Meredith C; Doran-Peterson, Joy

    2012-08-01

    The USA has proposed that 30 % of liquid transportation fuel be produced from renewable resources by 2030 (Perlack and Stokes 2011). It will be impossible to reach this goal using corn kernel-based ethanol alone. Pectin-rich biomass, an under-utilized waste product of the sugar and juice industry, can augment US ethanol supplies by capitalizing on this already established feedstock. Currently, pectin-rich biomass is sold (at low value) as animal feed. This review focuses on the three most studied types of pectin-rich biomass: sugar beet pulp, citrus waste and apple pomace. Fermentations of these materials have been conducted with a variety of ethanologens, including yeasts and bacteria. Escherichia coli can ferment a wide range of sugars including galacturonic acid, the primary component of pectin. However, the mixed acid metabolism of E. coli can produce unwanted side products. Saccharomyces cerevisiae cannot naturally ferment galacturonic acid nor pentose sugars but has a homoethanol pathway. Erwinia chrysanthemi is capable of degrading many of the cell wall components of pectin-rich materials, including pectin. Klebsiella oxytoca can metabolize a diverse array of sugars including cellobiose, one degradation product of cellulose. However, both E. chrysanthemi and K. oxytoca produce side products during fermentation, similar to E. coli. Using pectin-rich residues from industrial processes is beneficial because the material is already collected and partially pretreated to facilitate enzymatic deconstruction of the plant cell walls. Using biomass already produced for other purposes is an attractive practice because fewer greenhouse gases (GHG) will be anticipated from land-use changes. PMID:22695801

  13. Process Design and Economics for the Conversion of Algal Biomass to Biofuels: Algal Biomass Fractionation to Lipid- and Carbohydrate-Derived Fuel Products

    SciTech Connect

    Davis, R.; Kinchin, C.; Markham, J.; Tan, E.; Laurens, L.; Sexton, D.; Knorr, D.; Schoen, P.; Lukas, J.

    2014-09-01

    Beginning in 2013, NREL began transitioning from the singular focus on ethanol to a broad slate of products and conversion pathways, ultimately to establish similar benchmarking and targeting efforts. One of these pathways is the conversion of algal biomass to fuels via extraction of lipids (and potentially other components), termed the 'algal lipid upgrading' or ALU pathway. This report describes in detail one potential ALU approach based on a biochemical processing strategy to selectively recover and convert select algal biomass components to fuels, namely carbohydrates to ethanol and lipids to a renewable diesel blendstock (RDB) product. The overarching process design converts algal biomass delivered from upstream cultivation and dewatering (outside the present scope) to ethanol, RDB, and minor coproducts, using dilute-acid pretreatment, fermentation, lipid extraction, and hydrotreating.

  14. Acid-Catalyzed Algal Biomass Pretreatment for Integrated Lipid and Carbohydrate-Based Biofuels Production

    DOE PAGESBeta

    Laurens, L. M. L.; Nagle, N.; Davis, R.; Sweeney, N.; Van Wychen, S.; Lowell, A.; Pienkos, P. T.

    2014-11-12

    One of the major challenges associated with algal biofuels production in a biorefinery-type setting is improving biomass utilization in its entirety, increasing the process energetic yields and providing economically viable and scalable co-product concepts. We demonstrate the effectiveness of a novel, integrated technology based on moderate temperatures and low pH to convert the carbohydrates in wet algal biomass to soluble sugars for fermentation, while making lipids more accessible for downstream extraction and leaving a protein-enriched fraction behind. We studied the effect of harvest timing on the conversion yields, using two algal strains; Chlorella and Scenedesmus, generating biomass with distinctive compositionalmore » ratios of protein, carbohydrate, and lipids. We found that the late harvest Scenedesmus biomass had the maximum theoretical biofuel potential at 143 gasoline gallon equivalent (GGE) combined fuel yield per dry ton biomass, followed by late harvest Chlorella at 128 GGE per ton. Our experimental data show a clear difference between the two strains, as Scenedesmus was more successfully converted in this process with a demonstrated 97 GGE per ton. Our measurements indicated a release of >90% of the available glucose in the hydrolysate liquors and an extraction and recovery of up to 97% of the fatty acids from wet biomass. Techno-economic analysis for the combined product yields indicates that this process exhibits the potential to improve per-gallon fuel costs by up to 33% compared to a lipids-only process for one strain, Scenedesmus, grown to the mid-point harvest condition.« less

  15. Characterization of Spirulina biomass for CELSS diet potential

    NASA Technical Reports Server (NTRS)

    Tadros, Mahasin G.

    1988-01-01

    Spirulina sp. as a bioregenerative photosynthetic and an edible alga for space craft crew in a CELSS, was characterized for growth rate and biomass yield in batch cultures, under various environmental conditions. The cell characteristics were identified for two strains of Spirulina: S. maxima and S. plantensis. Fast growth rate and high yield of both strains were obtained under the following conditions: temperature (30 to 35 C), light irradiance (60 to 100 uE/m/s), nitrate (30 mM), phosphate (2 mM), aeration (300 ml/min), and ph (9 to 10). The partitioning of the assimalatory products (proteins, carbohydrates, lipids) were manipulated by varying the environmental growth conditions. The experiments with Spirulina demonstrated that under stress conditions (high light 120 uE/m/s, temperature 38 C, nitrogen or phosphate limitation; 0.1 M sodium chloride) carbohydrate increased at the expense of protein. In other experiments, where the growth media were sufficient in nutrients and incubated under optimum growth conditions, the total proteins were increased up to almost 70 percent of the organic weight. Conclusion: The nutritional quality of the alga could be manipulated by growth conditions, and therefore usful as a subsystem in CELSS.

  16. Biomass production and nitrogen dynamics in an integrated aquaculture/agriculture system

    NASA Technical Reports Server (NTRS)

    Owens, L. P.; Hall, C. R.

    1990-01-01

    A combined aquaculture/agriculture system that brings together the three major components of a Controlled Ecological Life Support System (CELSS) - biomass production, biomass processing, and waste recycling - was developed to evaluate ecological processes and hardware requirements necessary to assess the feasibility of and define design criteria for integration into the Kennedy Space Center (KSC) Breadboard Project. The system consists of a 1 square meter plant growth area, a 500 liter fish culture tank, and computerized monitoring and control hardware. Nutrients in the hydrophonic solution were derived from fish metabolites and fish food leachate. In five months of continuous operation, 27.0 kg of lettuce tops, 39.9 kg of roots and biofilm, and 6.6 kg of fish (wet weights) were produced with 12.7 kg of fish food input. Based on dry weights, a biomass conversion index of 0.52 was achieved. A nitrogen budget was derived to determine partitioning of nitrogen within various compartments of the system. Accumulating nitrogen in the hypoponic solution indicated a need to enlarge the plant growth area, potentially increasing the biomass production and improving the biomass conversion index.

  17. Biomass energy production. 1975-April, 1980 (citations from the International Aerospace Abstracts Data Base). Report for 1975-Apr 80

    SciTech Connect

    Moore, P.W.

    1980-06-01

    These citations from the international literature describe the production and/or utilization of most forms of biomass as a source of energy, fuel, food, and chemical intermediates or feedstocks. Biomass conversion by incineration, gasification, pyrolysis, hydrolysis, anaerobic digestion, or fermentation, as well as by catalytic, photosynthetic, chemosynthetic, and bio-electrochemical means are among the conversion processes considered. Discussions include biomass plantation and material productivity, transportation and equipment requirements, environmental effects, comparisons of means and efficiencies of utilization and conversion, assessments of limitations, and evaluations of economic potential. (Contains 210 abstracts)

  18. Methods for producing and using densified biomass products containing pretreated biomass fibers

    SciTech Connect

    Dale, Bruce E.; Ritchie, Bryan; Marshall, Derek

    2015-05-26

    A process is provided comprising subjecting a quantity of plant biomass fibers to a pretreatment to cause at least a portion of lignin contained within each fiber to move to an outer surface of said fiber, wherein a quantity of pretreated tacky plant biomass fibers is produced; and densifying the quantity of pretreated tacky plant biomass fibers to produce one or more densified biomass particulates, wherein said biomass fibers are densified without using added binder.

  19. Kinetics study on biomass pyrolysis for fuel gas production.

    PubMed

    Chen, Guan-Yi; Fang, Meng-Xiang; Andries, J; Luo, Zhong-Yang; Spliethoff, H; Cen, Ke-Fa

    2003-01-01

    Kinetic knowledge is of great importance in achieving good control of the pyrolysis and gasification process and optimising system design. An overall kinetic pyrolysis scheme is therefore addressed here. The kinetic modelling incorporates the following basic steps: the degradation of the virgin biomass materials into primary products (tar, gas and semi-char), the decomposition of primary tar into secondary products and the continuous interaction between primary gas and char. The last step is disregarded completely by models in the literature. Analysis and comparison of predicted results from different kinetic schemes and experimental data on our fixed bed pyrolyser yielded very positive evidence to support our kinetic scheme. PMID:12861621

  20. Biogas production from anaerobic digestion of Spirulina maxima algal biomass

    SciTech Connect

    Rejean Samson; Anh LeDuy

    1982-08-01

    Spirulina maxima algal biomass could be used as the sole nutrient for the production of biogas by anaerobic digestion process. It is relatively simple to adapt the municipal sewage sludge to this new substrate. The adapted sludge is very stable. Under nonoptimal conditions, the methane yield and productivity obtained were 0.26 m/sup 3//(kg VS added day) and 0.26 m/sup 3//(kg VS added day), respectively, with the semicontinuous, daily fed, anaerobic digestion having loading rate of 0.97 kg VS/(m/sup 3/ day), retention time of 33 days and temperature of 30/sup 0/C.

  1. Biomass production efficiency controlled by management in temperate and boreal ecosystems

    NASA Astrophysics Data System (ADS)

    Campioli, M.; Vicca, S.; Luyssaert, S.; Bilcke, J.; Ceschia, E.; Chapin, F. S., III; Ciais, P.; Fernández-Martínez, M.; Malhi, Y.; Obersteiner, M.; Olefeldt, D.; Papale, D.; Piao, S. L.; Peñuelas, J.; Sullivan, P. F.; Wang, X.; Zenone, T.; Janssens, I. A.

    2015-11-01

    Plants acquire carbon through photosynthesis to sustain biomass production, autotrophic respiration and production of non-structural compounds for multiple purposes. The fraction of photosynthetic production used for biomass production, the biomass production efficiency, is a key determinant of the conversion of solar energy to biomass. In forest ecosystems, biomass production efficiency was suggested to be related to site fertility. Here we present a database of biomass production efficiency from 131 sites compiled from individual studies using harvest, biometric, eddy covariance, or process-based model estimates of production. The database is global, but dominated by data from Europe and North America. We show that instead of site fertility, ecosystem management is the key factor that controls biomass production efficiency in terrestrial ecosystems. In addition, in natural forests, grasslands, tundra, boreal peatlands and marshes, biomass production efficiency is independent of vegetation, environmental and climatic drivers. This similarity of biomass production efficiency across natural ecosystem types suggests that the ratio of biomass production to gross primary productivity is constant across natural ecosystems. We suggest that plant adaptation results in similar growth efficiency in high- and low-fertility natural systems, but that nutrient influxes under managed conditions favour a shift to carbon investment from the belowground flux of non-structural compounds to aboveground biomass.

  2. Biogas energy production from tropical biomass wastes by anaerobic digestion.

    PubMed

    Ge, Xumeng; Matsumoto, Tracie; Keith, Lisa; Li, Yebo

    2014-10-01

    Anaerobic digestion (AD) is an attractive technology in tropical regions for converting locally abundant biomass wastes into biogas which can be used to produce heat, electricity, and transportation fuels. However, investigations on AD of tropical forestry wastes, such as albizia biomass and food wastes, such as taro, papaya, and sweet potato, are limited. In this study, these tropical biomass wastes were evaluated for biogas production by liquid AD (L-AD) and/or solid-state AD (SS-AD), depending on feedstock characteristics. When albizia leaves and chips were used as feedstocks, L-AD had greater methane yields (161 and 113 L kg(-1)VS, respectively) than SS-AD (156.8 and 59.6 L kg(-1)VS, respectively), while SS-AD achieved 5-fold higher volumetric methane productivity than L-AD. Mono-digestion and co-digestion of taro skin, taro flesh, papaya, and sweet potato achieved methane yields from 345 to 411 L kg(-1)VS, indicating the robustness of AD technology. PMID:25022835

  3. Method for producing ethanol and co-products from cellulosic biomass

    SciTech Connect

    Nguyen, Quang A

    2013-10-01

    The present invention generally relates to processes for production of ethanol from cellulosic biomass. The present invention also relates to production of various co-products of preparation of ethanol from cellulosic biomass. The present invention further relates to improvements in one or more aspects of preparation of ethanol from cellulosic biomass including, for example, improved methods for cleaning biomass feedstocks, improved acid impregnation, and improved steam treatment, or "steam explosion."

  4. Phycoremediation coupled production of algal biomass, harvesting and anaerobic digestion: possibilities and challenges.

    PubMed

    Prajapati, Sanjeev Kumar; Kaushik, Prachi; Malik, Anushree; Vijay, Virendra Kumar

    2013-12-01

    Biogas produced from anaerobic digestion is a versatile and environment friendly fuel which traditionally utilizes cattle dung as the substrate. In the recent years, owing to its high content of biodegradable compounds, algal biomass has emerged as a potential feedstock for biogas production. Moreover, the ability of algae to treat wastewater and fix CO2 from waste gas streams makes it an environmental friendly and economically feasible feedstock. The present review focuses on the possibility of utilizing wastewater as the nutrient and waste gases as the CO2 source for algal biomass production and subsequent biogas generation. Studies describing the various harvesting methods of algal biomass as well as its anaerobic digestion have been compiled and discussed. Studies targeting the most recent advancements on biogas enrichment by algae have been discussed. Apart from highlighting the various advantages of utilizing algal biomass for biogas production, limitations of the process such as cell wall resistivity towards digestion and inhibitions caused due to ammonia toxicity and the possible strategies for overcoming the same have been reviewed. The studies compiled in the present review indicate that if the challenges posed in translating the lab scale studies on phycoremediation and biogas production to pilot scale are overcome, algal biogas could become the sustainable and economically feasible source of renewable energy. PMID:23827782

  5. Evaluation of Biomass Availability for Biogas Production at Regional Level

    NASA Astrophysics Data System (ADS)

    Dzene, I.; Bodescu, F.

    2009-01-01

    Currently available data were used in an integrated deterministic modelling approach to assess the total biomass availability. The conceptual approach of combining the benefits of relational database and GIS modelling was tested in two eastern European countries - in Latvia and Romania, both located in different bio-geographical regions. The developed system has proven its efficiency in dealing with heterogeneity in different levels of complexity regarding environmental and ecological structures. The overall approach of assessing the biomass resources was first to estimate the quantity of material generated from municipal waste and agricultural practices in each of research areas. The quantity of material that could be recovered from these practices was then taken into account and the technical and environmental constraints associated with other site factors were evaluated. As a result, the particular areas with high, medium and low potential in each country were identified.

  6. Development of a Fluorescence-based Method for Monitoring Glucose Catabolism and its Potential use in a Biomass Hydrolysis Assay

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Availability and low cost of lignocellulosic biomass has caused tremendous interest in the fermentation of lignocellulosic-derived sugars for the production of liquid fuels. The economic feasibility of lignocellulosic biofuels can be improved by evaluating the fermentation potential of different fee...

  7. Cellulolytic and xylanolytic potential of high β-glucosidase-producing Trichoderma from decaying biomass.

    PubMed

    Okeke, Benedict C

    2014-10-01

    Availability, cost, and efficiency of microbial enzymes for lignocellulose bioconversion are central to sustainable biomass ethanol technology. Fungi enriched from decaying biomass and surface soil mixture displayed an array of strong cellulolytic and xylanolytic activities. Strains SG2 and SG4 produced a promising array of cellulolytic and xylanolytic enzymes including β-glucosidase, usually low in cultures of Trichoderma species. Nucleotide sequence analysis of internal transcribed spacer 2 (ITS2) region of rRNA gene revealed that strains SG2 and SG4 are closely related to Trichoderma inhamatum, Trichoderma piluliferum, and Trichoderma aureoviride. Trichoderma sp. SG2 crude culture supernatant correspondingly displayed as much as 9.84 ± 1.12, 48.02 ± 2.53, and 30.10 ± 1.11 units mL(-1) of cellulase, xylanase, and β-glucosidase in 30 min assay. Ten times dilution of culture supernatant of strain SG2 revealed that total activities were about 5.34, 8.45, and 2.05 orders of magnitude higher than observed in crude culture filtrate for cellulase, xylanase, and β-glucosidase, respectively, indicating that more enzymes are present to contact with substrates in biomass saccharification. In parallel experiments, Trichoderma species SG2 and SG4 produced more β-glucosidase than the industrial strain Trichoderma reesei RUT-C30. Results indicate that strains SG2 and SG4 have potential for low cost in-house production of primary lignocellulose-hydrolyzing enzymes for production of biomass saccharides and biofuel in the field. PMID:25129039

  8. Microbial biodiesel production by direct methanolysis of oleaginous biomass.

    PubMed

    Thliveros, Panagiotis; Uçkun Kiran, Esra; Webb, Colin

    2014-04-01

    Biodiesel is usually produced by the transesterification of vegetable oils and animal fats with methanol, catalyzed by strong acids or bases. This study introduces a novel biodiesel production method that features direct base-catalyzed methanolysis of the cellular biomass of oleaginous yeast Rhodosporidium toruloides Y4. NaOH was used as catalyst for transesterification reactions and the variables affecting the esterification level including catalyst concentration, reaction temperature, reaction time, solvent loading (methanol) and moisture content were investigated using the oleaginous yeast biomass. The most suitable pretreatment condition was found to be 4gL(-1) NaOH and 1:20 (w/v) dried biomass to methanol ratio for 10h at 50°C and under ambient pressure. Under these conditions, the fatty acid methyl ester (FAME) yield was 97.7%. Therefore, the novel method of direct base-catalyzed methanolysis of R. toruloides is a much simpler, less tedious and time-consuming, process than the conventional processes with higher FAME (biodiesel) conversion yield. PMID:24556371

  9. Biomass and pigments production in photosynthetic bacteria wastewater treatment: Effects of photoperiod.

    PubMed

    Zhou, Qin; Zhang, Panyue; Zhang, Guangming; Peng, Meng

    2015-08-01

    This study aimed at enhancing the bacterial biomass and pigments production in together with pollution removal in photosynthetic bacteria (PSB) wastewater treatment via using different photoperiods. Different light/dark cycles and light/dark cycle frequencies were examined. Results showed that PSB had the highest biomass production, COD removal and biomass yield, and light energy efficiency with light/dark cycle of 2h/1h. The corresponding biomass, COD removal and biomass yield reached 2068mg/L, 90.3%, and 0.38mg-biomass/mg-COD-removal, respectively. PSB showed higher biomass production and biomass yield with higher light/dark cycle frequency. Mechanism analysis showed within a light/dark cycle from 1h/2h to 2h/1h, the carotenoid and bacteriochlorophyll production increased with an increase in light/dark cycle. Moreover, the pigment contents were much higher with lower frequency of 2-4 times/d. PMID:25958142

  10. Growth, yield and compositional characteristics of Jerusalem artichoke as it relates to biomass production

    SciTech Connect

    Stauffer, M.D.; Chubey, B.B.; Dorrell, D.G.

    1980-01-01

    Jerusalem artichoke (Helianthus tuberosus L.) has shown excellent potential as a carbohydrate-rich crop. Initial investigations determined inulin and tuber yields; however, when additional studies showed that good quality pulp remained after inulin extraction and high forage yields per hectare were obtainable, the scope of investigation was broadened to assess utilization of the total plant. Plant growth, yield and compositional characteristics of Jerusalem artichoke as they relate to biomass production will be reported.

  11. Current technologies, economics, and perspectives for 2,5-dimethylfuran production from biomass-derived intermediates.

    PubMed

    Saha, Basudeb; Abu-Omar, Mahdi M

    2015-04-13

    Since the U.S. Department of Energy (DOE) published a perspective article that described the potential of the top ten biomass-derived platform chemicals as petroleum replacements for high-value commodity and specialty chemicals, researchers around the world have been motivated to develop technologies for the conversion of biomass and biomass-derived intermediates into chemicals and fuels. Among several biorefinery processes, the conversion of biomass carbohydrates into 2,5-dimethylfuran (DMF) has received significant attention because of its low oxygen content, high energy content, and high octane value. DMF can further serve as a petroleum-replacement, biorenewable feedstock for the production of p-xylene (pX). In this review, we aim specifically to present a concise and up-to-date analysis of DMF production technologies with a critical discussion on catalytic systems, mechanistic insight, and process economics, which includes sensitivity analysis, so that more effective catalysts can be designed. Special emphasis has been given to bifunctional catalysts that improve DMF yields and selectivity and the synergistic effect of the bifunctional sites. Process economics for the current processes and the scope for further improvement are discussed. It is anticipated that the chemistry detailed in this review will guide researchers to develop more practical catalytic processes to enable the economic production of bio-based DMF. Processes for the upgrade of DMF to pX are also described. PMID:25703838

  12. Performance and reliability of the NASA biomass production chamber

    NASA Technical Reports Server (NTRS)

    Fortson, R. E.; Sager, J. C.; Chetirkin, P. V.

    1994-01-01

    The Biomass Production Chamber (BPC) at the Kennedy Space Center is part of the Controlled Ecological Life Support System (CELSS) Breadboard Project. Plants are grown in a closed environment in an effort to quantify their contributions to the requirements for life support. Performance of this system is described. Also, in building this system, data from component and subsystem failures are being recorded. These data are used to identify problem areas in the design and implementation. The techniques used to measure the reliability will be useful in the design and construction of future CELSS. Possible methods for determining the reliability of a green plant, the primary component of CELSS, are discussed.

  13. Design and performance of the KSC Biomass Production Chamber

    NASA Technical Reports Server (NTRS)

    Prince, Ralph P.; Knott, William M.; Sager, John C.; Hilding, Suzanne E.

    1987-01-01

    NASA's Controlled Ecological Life Support System program has instituted the Kennedy Space Center 'breadboard' project of which the Biomass Production Chamber (BPC) presently discussed is a part. The BPC is based on a modified hypobaric test vessel; its design parameters and operational parameters have been chosen in order to meet a wide range of plant-growing objectives aboard future spacecraft on long-duration missions. A control and data acquisition subsystem is used to maintain a common link between the heating, ventilation, and air conditioning system, the illumination system, the gas-circulation system, and the nutrient delivery and monitoring subsystems.

  14. Environmental impacts of conversion of cropland to biomass production

    SciTech Connect

    Green, T.H.; Brown, G.F.; Bingham, L.

    1996-12-31

    A study was initiated to determine the effects of conversion of row crop land to biomass production on runoff quality and quantity. Treatments were: (1) remain in row crop (no-till corn); (2) convert to short rotation woody crop (SRWC) production with sweetgum (Liquidambar styraciflua L.) planted in a 1.5 in by 3 in spacing maintaining complete weed control; (3) convert to SRWC with a tall fescue (Festuca eliator L.) cover crop planted in a 2.4 in strip centered between rows of trees to reduce erosion; and (4) convert to switchgrass (Panicum virgatum L.) as a biomass energy crop. Plots within a block similar in size (approximately 0.45 ha in block 1 and 0.20 ha in block 2), slope, soils, topographic position, recent land use history, etc. Although switchgrass plots eroded more early in the growing season, erosion was low once it became well established. Conversely, plots where trees were grown with no cover continued to erode throughout the growing season. These results indicate that growing short-rotation intensively cultured hardwoods with complete weed control will provide little erosion relief in agricultural fields, at least during the first growing season. Planting switchgrass for bioenergy production, however, does protect the soil. Nutrient runoff was related to fertilization.

  15. Liquid hot water pretreatment of lignocellulosic biomass for bioethanol production accompanying with high valuable products.

    PubMed

    Zhuang, Xinshu; Wang, Wen; Yu, Qiang; Qi, Wei; Wang, Qiong; Tan, Xuesong; Zhou, Guixiong; Yuan, Zhenhong

    2016-01-01

    Pretreatment is an essential prerequisite to overcome recalcitrance of biomass and enhance the ethanol conversion efficiency of polysaccharides. Compared with other pretreatment methods, liquid hot water (LHW) pretreatment not only reduces the downstream pressure by making cellulose more accessible to the enzymes but minimizes the formation of degradation products that inhibit the growth of fermentative microorganisms. Herein, this review summarized the improved LHW process for different biomass feedstocks, the decomposition behavior of biomass in the LHW process, the enzymatic hydrolysis of LHW-treated substrates, and production of high value-added products and ethanol. Moreover, a combined process producing ethanol and high value-added products was proposed basing on the works of Guangzhou Institute of Energy Conversion to make LHW pretreatment acceptable in the biorefinery of cellulosic ethanol. PMID:26403722

  16. Landscape and species diversity: optimizing the use of land and biomass species for biofuel feedstock production systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biomass crops have the potential to produce a variety of products for use in the expanding bioeconomy. Numerous perennial plant species have been identified to serve as dedicated and custom-tailored feedstocks for the production of bioenergy and bioproducts, while also providing numerous positive en...

  17. Sustainable production of bioenergy and bio-char from the straw of high biomass soybean lines via fast pyrolysis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The straws of two high-biomass soybean lines developed at ARS for bioenergy were subjected to thermochemical conversion by fast pyrolysis. The objective was to evaluate the potential use of the straw for the production of liquid fuel intermediates that can be burned “as is” and/or potentially upgra...

  18. Physicochemical factors differentially affect the biomass and bacteriocin production by bovine Enterococcus mundtii CRL1656.

    PubMed

    Espeche, M Carolina; Juárez Tomás, M Silvina; Wiese, Birgitt; Bru, Elena; Nader-Macías, M E Fátima

    2014-02-01

    Bovine Enterococcus mundtii CRL1656 (Centro de Referencia para Lactobacilos Culture Collection) produces an anti-Listeria and anti-Streptococcus dysgalactiae bacteriocin identified as mundticin CRL1656. The strain and its bacteriocin are candidates to be included in a beneficial product to prevent bovine mastitis as an alternative to antimicrobial agents. To optimize the production of biomass and mundticin CRL1656 by E. mundtii CRL1656, a complete 3 × 2(4) factorial design was applied. The effect of culture medium, initial pH, inoculum size, incubation temperature, and agitation conditions on biomass and bacteriocin production was evaluated simultaneously. Growth parameters were determined using the modified Gompertz model. A nonlinear model was used to estimate the effects of the variables on growth parameters. Bacteriocin production was analyzed using a linear mixed model. Optimal biomass and mundticin CRL1656 production by E. mundtii CRL1656 were obtained in different conditions. Maximal growth was recorded in autolyzed yeast, peptone, tryptone, Tween 80, and glucose or M17 broths, pH 6.5, 5.0% inoculum, 30 °C, with agitation. However, bacteriocin titers were higher in autolyzed yeast, peptone, tryptone, Tween 80, and glucose or de Man-Rogosa-Sharpe (MRS) broths, pH 6.5, 30°C, both with or without agitation. Knowledge of the optimum conditions for growth and bacteriocin production of E. mundtii CRL1656 will allow the obtainment of high levels of biomass and mundticin CRL1656 as bioingredients of potential products to prevent bovine mastitis. PMID:24359825

  19. Biomass alcohols as potential petroleum alternatives in the fuel and petrochemical industries: A generalized network model

    NASA Astrophysics Data System (ADS)

    Farina, R. F.

    A generalized network model called PETNET is developed to address this problem. The focus of the analysis presented is the role of biomass alcohols as potential alternatives to fossil hydrocarbons as raw materials in the petrochemical and oil industries. Illustrative scenarios for biomass-based alcohol replacements are investigated with PETNET by solving for alternative assumptions of price, capacity, resource availability and process technology.

  20. Kinetic studies of amylase and biomass production by Calvatia gigantea

    SciTech Connect

    Kekos, D.; Macris, B.J.

    1987-01-01

    Production of alpha-amylase (alpha-4, glucan 4-glucanohydrolase, EC 3.2.1.1) by microorganisms has been practiced for many years in small and large scale operations and the literature on this enzyme is voluminous. Aspergillus niger and Aspergillus oryzae have been reported as the main fungal species used for commercial production of the enzyme. On the other hand, large volumes of low-cost agricultural products such as acorn (the perisperm-free dry seed contains approximately 60% starch) are wasted in many countries and provide a challenge to biotechnology to efficiently utilize these rich sources of starch for the production of high added value products like enzymes. C. gigantea is an edible puffball excreting high levels of alpha-amylase when cultivated on different sources of starch containing elevated quantities of toxic tannic compounds. This fungus has been employed for the production of microbial protein from wastes and acorns containing high levels of toxic tannic compounds. The same fungus was also reported to grow on both hydrolyzable and condensed tannins as sole carbon sources. The present work was undertaken to investigate certain kinetic characteristics of alpha-amylase and biomass production by C. gigantea grown on soluble and acorn starch in a lab fermenter. (Refs. 18).

  1. Value added liquid products from waste biomass pyrolysis using pretreatments.

    PubMed

    Das, Oisik; Sarmah, Ajit K

    2015-12-15

    Douglas fir wood, a forestry waste, was attempted to be converted into value added products by pretreatments followed by pyrolysis. Four different types of pretreatments were employed, namely, hot water treatment, torrefaction, sulphuric acid and ammonium phosphate doping. Subsequently, pyrolysis was done at 500°C and the resulting bio-oils were analysed for their chemical composition using Karl Fischer titration, thermogravimetry, ion exchange, and gas chromatography. Pretreatment with acid resulted in the highest yield of bio-oil (~60%). The acid and salt pretreatments were responsible for drastic reduction in the lignin oligomers and enhancement of water content in the pyrolytic liquid. The quantity of xylose/mannose reduced as a result of pretreatments. Although, the content of fermentable sugars remained similar across all the pretreatments, the yield of levoglucosan increased. Pretreatment of the biomass with acid yielded the highest amount of levoglucosan in the bio-oil (13.21%). The acid and salt pretreatments also elevated the amount of acetic acid in the bio-oils. Addition of acid and salt to the biomass altered the interaction of cellulose-lignin in the pyrolysis regime. Application of pretreatments should be based on the intended end use of the liquid product having a desired chemical composition. PMID:26298257

  2. A Life Cycle Assessment on a Fuel Production Through Distributed Biomass Gasification Process

    NASA Astrophysics Data System (ADS)

    Dowaki, Kiyoshi; Eguchi, Tsutomu; Ohkubo, Rui; Genchi, Yutaka

    In this paper, we estimated life cycle inventories (energy intensities and CO2 emissions) on the biomass gasification CGS, Bio-H2, Bio-MeOH (methanol) and Bio-DME (di-methyl ether), using the bottom-up methodology. CO2 emissions and energy intensities on material's chipping, transportation and dryer operation were estimated. Also, the uncertainties on the moisture content of biomass materials and the transportation distance to the plant were considered by the Monte Carlo simulation. The energy conversion system was built up by gasification through the BLUE Tower process, with either CGS, PSA (Pressure Swing Absorption) system or the liquefaction process. In our estimation, the biomass materials were the waste products from Japanese Cedar. The uncertainties of moisture content and transportation distance were assumed to be 20 to 50 wt.% and 5 to 50 km, respectively. The capability of the biomass gasification plant was 10 t-dry/d, that is, an annual throughput of 3,000 t-dry/yr. The production energy in each case was used as a functional unit. Finally, the energy intensities of 1.12 to 3.09 MJ/MJ and CO2 emissions of 4.79 to 88.0 g-CO2/MJ were obtained. CGS case contributes to the environmental mitigation, and Bio-H2 and/or Bio-DME cases have a potential to reduce CO2 emissions, compared to the conventional ones.

  3. Influence of starch on microalgal biomass recovery, settleability and biogas production.

    PubMed

    Gutiérrez, Raquel; Ferrer, Ivet; García, Joan; Uggetti, Enrica

    2015-06-01

    In the context of wastewater treatment with microalgae cultures, coagulation-flocculation followed by sedimentation is one of the suitable options for microalgae harvesting. This process is enabled by the addition of chemicals (e.g. iron). However, in a biorefinery perspective, it is important to avoid possible contamination of downstream products caused by chemicals addition. The aim of this study was to evaluate the effect of potato starch as flocculant for microalgal biomass coagulation-flocculation and sedimentation. The optimal flocculant dose (25mg/L) was determined with jar tests. Such a concentration led to more than 95% biomass recovery (turbidity<9NTU). The settleability of flocs was studied using an elutriation apparatus measuring the settling velocities distribution. This test underlined the positive effect of starch on the biomass settling velocity, increasing to >70% the percentage of particles with settling velocities >6.5m/h. Finally, biochemical methane potential tests showed that starch biodegradation increased the biogas production from harvested biomass. PMID:25795448

  4. Natural products and altered derivatives as tracers for biomass combustion in aerosols

    SciTech Connect

    Simoneit, B.R.T.; Radzi bin Abas, M.; Cass, G.R.

    1995-12-01

    Biomass combustion is an important primary source of carbonaceous particles in the global atmosphere. Various molecular markers have been proposed for this process but additional specific tracers are needed. The injection of natural product organic compounds into smoke occurs primarily by direct volatilization/steam stripping and by pyrolysis. Although the composition of organic matter in smoke particles is highly variable, the molecular structures of the tracers are generally source specific. Homologous compounds and biomarkers present in smoke are derived directly from plant wax, gum and resin by volatilization and secondarily from pyrolysis of biopolymers (e.g., lignin, cutin, suberin), wax, gum and resin. The component complexity is illustrated with examples from controlled bums of temperate and tropical biomass fuels. Conifer smoke contains characteristic tracers from diterpenoids as well as phenolics and other oxygenated species. These are recognizable in urban airsheds. The major organic components of smoke from tropical biomass are straight-chain, aliphatic and oxygenated compounds and triterpenoids. Several compounds are potential key indicators for combustion of such biomass. The precursor to product approach of organic geochemistry can be applied successfully to provide molecular tracers for studying smoke plume chemistry and dispersion.

  5. Recycling of lipid-extracted hydrolysate as nitrogen supplementation for production of thraustochytrid biomass.

    PubMed

    Lowrey, Joshua; Armenta, Roberto E; Brooks, Marianne S

    2016-08-01

    Efficient resource usage is important for cost-effective microalgae production, where the incorporation of waste streams and recycled water into the process has great potential. This study builds upon emerging research on nutrient recycling in thraustochytrid production, where waste streams are recovered after lipid extraction and recycled into future cultures. This research investigates the nitrogen flux of recycled hydrolysate derived from enzymatic lipid extraction of thraustochytrid biomass. Results indicated the proteinaceous content of the recycled hydrolysate can offset the need to supply fresh nitrogen in a secondary culture, without detrimental impact upon the produced biomass. The treatment employing the recycled hydrolysate with no nitrogen addition accumulated 14.86 g L(-1) of biomass in 141 h with 43.3 % (w/w) lipid content compared to the control which had 9.26 g L(-1) and 46.9 % (w/w), respectively. This improved nutrient efficiency and wastewater recovery represents considerable potential for enhanced resource efficiency of commercial thraustochytrid production. PMID:27155854

  6. Scenedesmus dimorphus biofilm: Photoefficiency and biomass production under intermittent lighting

    PubMed Central

    Toninelli, Andrea Efrem; Wang, Junfeng; Liu, Mingshen; Wu, Hong; Liu, Tianzhong

    2016-01-01

    This study investigated the effect of intermittent lighting on the growth performances of a Scenedesmus dimorphus biofilm. Flashing light effect (FLE) is common in traditional suspended cultures of microalgae; yet, publications about this phenomenon, in the context of biofilm cultivation, are scarce. In this work we demonstrate that, thanks to intermittent illumination, it is possible for attached cultivations to fulfill FLE, improve photoefficiency and productivity as well as providing protection from photoinhibition using much lower flashing light frequencies than those usually required with suspended cultures. Medium frequency intermittent lighting (0.1 Hz) guaranteed excellent light integration resulting in 9.13 g m−2 d−1 biomass productivity, which was 8.9% higher than with continuous lighting. Results showed that a light fraction value of 0.5 always improved photoefficiency values as related to continuous light with a 118.8% maximum increase. PMID:27561323

  7. Scenedesmus dimorphus biofilm: Photoefficiency and biomass production under intermittent lighting.

    PubMed

    Toninelli, Andrea Efrem; Wang, Junfeng; Liu, Mingshen; Wu, Hong; Liu, Tianzhong

    2016-01-01

    This study investigated the effect of intermittent lighting on the growth performances of a Scenedesmus dimorphus biofilm. Flashing light effect (FLE) is common in traditional suspended cultures of microalgae; yet, publications about this phenomenon, in the context of biofilm cultivation, are scarce. In this work we demonstrate that, thanks to intermittent illumination, it is possible for attached cultivations to fulfill FLE, improve photoefficiency and productivity as well as providing protection from photoinhibition using much lower flashing light frequencies than those usually required with suspended cultures. Medium frequency intermittent lighting (0.1 Hz) guaranteed excellent light integration resulting in 9.13 g m(-2) d(-1) biomass productivity, which was 8.9% higher than with continuous lighting. Results showed that a light fraction value of 0.5 always improved photoefficiency values as related to continuous light with a 118.8% maximum increase. PMID:27561323

  8. Biomass and lipid production of heterotrophic microalgae Chlorella protothecoides by using biodiesel-derived crude glycerol.

    PubMed

    Chen, Yen-Hui; Walker, Terry H

    2011-10-01

    Microalgal lipids may be a more sustainable biodiesel feedstock than crop oils. We have investigated the potential for using the crude glycerol as a carbon substrate. In batch mode, the biomass and lipid concentration of Chlorella protothecoides cultivated in a crude glycerol medium were, respectively, 23.5 and 14.6 g/l in a 6-day cultivation. In the fed-batch mode, the biomass and lipid concentration improved to 45.2 and 24.6 g/l after 8.2 days of cultivation, respectively. The maximum lipid productivity of 3 g/l day in the fed-batch mode was higher than that produced by batch cultivation. This work demonstrates the feasibility of crude biodiesel glycerol as an alternative carbon substrate to glucose for microalgal cultivation and a cost reduction of carbon substrate feed in microalgal lipid production may be expected. PMID:21691839

  9. Iron and sulfide oxidation within the basaltic ocean crust: implications for chemolithoautotrophic microbial biomass production

    NASA Astrophysics Data System (ADS)

    Bach, Wolfgang; Edwards, Katrina J.

    2003-10-01

    Microbial processes within the ocean crust are of potential importance in controlling rates of chemical reactions and thereby affecting chemical exchange between the oceans and lithosphere. We here assess the oxidation state of altered ocean crust and estimate the magnitude of microbial biomass production that might be supported by oxidative and nonoxidative alteration. Compilations of Fe 2O 3, FeO, and S concentrations from DSDP/ODP drill core samples representing upper basaltic ocean crust suggest that Fe 3+/ΣFe increases from 0.15 ± 0.05 to 0.45 ± 0.15 within the first 10-20 Myr of crustal evolution. Within the same time frame 70 ± 25% of primary sulfides in basalt are oxidized. With an annual production of 4.0 ± 1.8 × 10 15 g of upper (500 ± 200 m) crust and average initial concentrations of 8.0 ± 1.3 wt% Fe and 0.125 ± 0.020 wt% S, we estimate annual oxidation rates of 1.7 ± 1.2 × 10 12 mol Fe and 1.1 ± 0.7 × 10 11 mol S. We estimate that 50% of Fe oxidation may be attributed to hydrolysis, producing 4.5 ± 3.0 × 10 11 mol H 2/yr. Thermodynamic and bioenergetic calculations were used to estimate the potential chemolithoautotrophic microbial biomass production within ridge flanks. Combined, aerobic and anaerobic Fe and S oxidation may support production of up to 48 ± 21 × 10 10 g cellular carbon (C). Hydrogen-consuming reactions may support production of a similar or larger microbial biomass if iron reduction, nitrate reduction, or hydrogen oxidation by O 2(aq) are the prevailing metabolic reactions. If autotrophic sulfate reduction or methanogenesis prevail, the potential biomass production is 9 ± 7 × 10 10 g C/yr and 3 ± 2 × 10 10 g C/yr, respectively. Combined primary biomass production of up to ˜1 × 10 12 g C/yr may be similar to that fueled by anaerobic oxidation of organic matter in deep-seated heterotrophic systems. These estimates suggest that water-rock reactions may support significant microbial life within ridge flank

  10. Biomass Program Biopower Factsheet

    SciTech Connect

    2010-03-01

    Generating electricity and thermal energy from biomass has the potential to help meet national goals for renewable energy. The forest products industry has used biomass for power and heat for many decades, yet widespread use of biomass to supply electricity to the U.S. power grid and other applications is relatively recent.

  11. Emergent Patterns of Forest Biomass Production from Across and within a Micro-Network

    NASA Astrophysics Data System (ADS)

    Pederson, N.; Martin Benito, D.; Bishop, D. A.; Dawson, A.; Dietze, M.; Druckenbrod, D.; Dye, A.; Gonzalez, A. C.; Hessl, A. E.; Martin Fernandez, J.; McLachlan, J. S.; Paciorek, C. J.; Poulter, B.; Williams, J. W.

    2014-12-01

    maple's annual growth. Our micro-network gives insight to the many potential factors on the long-term development of and biomass production in forests.

  12. Process Design and Economics for the Conversion of Algal Biomass to Biofuels: Algal Biomass Fractionation to Lipid-and Carbohydrate-Derived Fuel Products

    SciTech Connect

    Davis, R.; Kinchin, C.; Markham, J.; Tan, E. C. D.; Laurens, L. M. L.; Sexton, D.; Knorr, D.; Schoen, P.; Lukas, J.

    2014-09-11

    The U.S. Department of Energy (DOE) promotes the production of a range of liquid fuels and fuel blendstocks from biomass feedstocks by funding fundamental and applied research that advances the state of technology in biomass production, conversion, and sustainability. As part of its involvement in this program, the National Renewable Energy Laboratory (NREL) investigates the conceptual production economics of these fuels. This includes fuel pathways from lignocellulosic (terrestrial) biomass, as well as from algal (aquatic) biomass systems.

  13. Remote sensing investigations of wetland biomass and productivity for global biosystems research

    NASA Technical Reports Server (NTRS)

    Harkisky, M.; Klemas, V.

    1983-01-01

    Monitoring biomass of wetlands ecosystems can provide information on net primary production and on the chemical and physical status of wetland soils relative to anaerobic microbial transformation of key elements. Multispectral remote sensing techniques successfully estimated macrophytic biomass in wetlands systems. Regression models developed from ground spectral data for predicting Spartina alterniflora biomass over an entire growing season include seasonal variations in biomass density and illumination intensity. An independent set of biomass and spectral data were collected and the standing crop biomass and net primary productivity were estimated. The improved spatial, radiometric and spectral resolution of th LANDSAT-4 Thematic Mapper over the LANDSAT MSS can greatly enhance multispectral techniques for estimating wetlands biomass over large areas. These techniques can provide the biomass data necessary for global ecology studies.

  14. Chronic nitrogen deposition alters tree allometric relationships: implications for biomass production and carbon storage.

    PubMed

    Ibáñez, Inés; Zak, Donald R; Burton, Andrew J; Pregitzer, Kurt S

    2016-04-01

    As increasing levels of nitrogen (N) deposition impact many terrestrial ecosystems, understanding the potential effects of higher N availability is critical for forecasting tree carbon allocation patterns and thus future forest productivity. Most regional estimates of forest biomass apply allometric equations, with parameters estimated from a limited number of studies, to forest inventory data (i.e., tree diameter). However most of these allometric equations cannot account for potential effects of increased N availability on biomass allocation patterns. Using 18 yr of tree diameter, height, and mortality data collected for a dominant tree species (Acer saccharum) in an atmospheric N deposition experiment, we evaluated how greater N availability affects allometric relationships in this species. After taking into account site and individual variability, our results reveal significant differences in allometric parameters between ambient and experimental N deposition treatments. Large trees under experimental N deposition reached greater heights at a given diameter; moreover, their estimated maximum height (mean ± standard deviation: 33.7 ± 0.38 m) was significantly higher than that estimated under the ambient condition (31.3 ± 0.31 m). Within small tree sizes (5-10 cm diameter) there was greater mortality under experimental N deposition, whereas the relative growth rates of small trees were greater under experimental N deposition. Calculations of stemwood biomass using our parameter estimates for the diameter-height relationship indicated the potential for significant biases in these estimates (~2.5%), with under predictions of stemwood biomass averaging 4 Mg/ha lower if ambient parameters were to be used to estimate stem biomass of trees in the experimental N deposition treatment. As atmospheric N deposition continues to increase into the future, ignoring changes in tree allometry will contribute to the uncertainty associated with aboveground carbon storage

  15. Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production.

    PubMed

    Liu, Fushan; Zhao, Qianru; Mano, Noel; Ahmed, Zaheer; Nitschke, Felix; Cai, Yinqqi; Chapman, Kent D; Steup, Martin; Tetlow, Ian J; Emes, Michael J

    2016-03-01

    We have identified a novel means to achieve substantially increased vegetative biomass and oilseed production in the model plant Arabidopsis thaliana. Endogenous isoforms of starch branching enzyme (SBE) were substituted by either one of the endosperm-expressed maize (Zea mays L.) branching isozymes, ZmSBEI or ZmSBEIIb. Transformants were compared with the starch-free background and with the wild-type plants. Each of the maize-derived SBEs restored starch biosynthesis but both morphology and structure of starch particles were altered. Altered starch metabolism in the transformants is associated with enhanced biomass formation and more-than-trebled oilseed production while maintaining seed oil quality. Enhanced oilseed production is primarily due to an increased number of siliques per plant whereas oil content and seed number per silique are essentially unchanged or even modestly decreased. Introduction of cereal starch branching isozymes into oilseed plants represents a potentially useful strategy to increase biomass and oilseed production in related crops and manipulate the structure and properties of leaf starch. PMID:26285603

  16. Productivity of wet soils: Biomass of cultivated and natural vegetation

    SciTech Connect

    Johnston, C.A.

    1988-12-01

    Wet soils, soils which have agronomic limitations because of excess water, comprise 105 million acres of non-federal land in the conterminous United States. Wet soils which support hydrophytic plants are ''wetlands'', and are some of the most productive natural ecosystems in the world. When both above- and belowground productivity are considered, cattail (Typha latifolia) is the most productive temperate wetland species (26.4 Mg/ha/year). Both cattail and reed (Phragmites australis) have aboveground productivities of about 13 Mg/ha/year. Although average aboveground yields of reed canarygrass (Phalaris arundinacea) are lower (9.5 Mg/ha/year), techniques for its establishment and cultivation are well-developed. Other herbaceous wetland species which show promise as biomass crops include sedge (Carex spp.), river bulrush (Scirpus fluviatilis) and prairie cordgrass (Spartina pectinata). About 40% of wet soils in the conterminous US are currently cultivated, and they produce one-quarter of the major US crops. Most of this land is artificially drained for crops such as corn, soybeans, and vegetables. US wetlands are drained for agriculture at the rate of 223,000 ha/yr. Paddies flooded with water are used to grow rice, cranberries, and wild rice. Forage and live sphagnum moss are products of undrained wetlands. A number of federal and state regulations apply to the draining or irrigation of wetlands, but most do not seriously restrict their use for agriculture. 320 refs., 36 tabs.

  17. The global economic long-term potential of modern biomass in a climate-constrained world

    NASA Astrophysics Data System (ADS)

    Klein, David; Humpenöder, Florian; Bauer, Nico; Dietrich, Jan Philipp; Popp, Alexander; Bodirsky, Benjamin Leon; Bonsch, Markus; Lotze-Campen, Hermann

    2014-07-01

    Low-stabilization scenarios consistent with the 2 °C target project large-scale deployment of purpose-grown lignocellulosic biomass. In case a GHG price regime integrates emissions from energy conversion and from land-use/land-use change, the strong demand for bioenergy and the pricing of terrestrial emissions are likely to coincide. We explore the global potential of purpose-grown lignocellulosic biomass and ask the question how the supply prices of biomass depend on prices for greenhouse gas (GHG) emissions from the land-use sector. Using the spatially explicit global land-use optimization model MAgPIE, we construct bioenergy supply curves for ten world regions and a global aggregate in two scenarios, with and without a GHG tax. We find that the implementation of GHG taxes is crucial for the slope of the supply function and the GHG emissions from the land-use sector. Global supply prices start at 5 GJ-1 and increase almost linearly, doubling at 150 EJ (in 2055 and 2095). The GHG tax increases bioenergy prices by 5 GJ-1 in 2055 and by 10 GJ-1 in 2095, since it effectively stops deforestation and thus excludes large amounts of high-productivity land. Prices additionally increase due to costs for N2O emissions from fertilizer use. The GHG tax decreases global land-use change emissions by one-third. However, the carbon emissions due to bioenergy production increase by more than 50% from conversion of land that is not under emission control. Average yields required to produce 240 EJ in 2095 are roughly 600 GJ ha-1 yr-1 with and without tax.

  18. Increased biomass productivity in green algae by tuning non-photochemical quenching.

    PubMed

    Berteotti, Silvia; Ballottari, Matteo; Bassi, Roberto

    2016-01-01

    Photosynthetic microalgae have a high potential for the production of biofuels and highly valued metabolites. However, their current industrial exploitation is limited by a productivity in photobioreactors that is low compared to potential productivity. The high cell density and pigment content of the surface layers of photosynthetic microalgae result in absorption of excess photons and energy dissipation through non-photochemical quenching (NPQ). NPQ prevents photoinhibition, but its activation reduces the efficiency of photosynthetic energy conversion. In Chlamydomonas reinhardtii, NPQ is catalyzed by protein subunits encoded by three lhcsr (light harvesting complex stress related) genes. Here, we show that heat dissipation and biomass productivity depends on LHCSR protein accumulation. Indeed, algal strains lacking two lhcsr genes can grow in a wide range of light growth conditions without suffering from photoinhibition and are more productive than wild-type. Thus, the down-regulation of NPQ appears to be a suitable strategy for improving light use efficiency for biomass and biofuel production in microalgae. PMID:26888481

  19. Increased biomass productivity in green algae by tuning non-photochemical quenching

    PubMed Central

    Berteotti, Silvia; Ballottari, Matteo; Bassi, Roberto

    2016-01-01

    Photosynthetic microalgae have a high potential for the production of biofuels and highly valued metabolites. However, their current industrial exploitation is limited by a productivity in photobioreactors that is low compared to potential productivity. The high cell density and pigment content of the surface layers of photosynthetic microalgae result in absorption of excess photons and energy dissipation through non-photochemical quenching (NPQ). NPQ prevents photoinhibition, but its activation reduces the efficiency of photosynthetic energy conversion. In Chlamydomonas reinhardtii, NPQ is catalyzed by protein subunits encoded by three lhcsr (light harvesting complex stress related) genes. Here, we show that heat dissipation and biomass productivity depends on LHCSR protein accumulation. Indeed, algal strains lacking two lhcsr genes can grow in a wide range of light growth conditions without suffering from photoinhibition and are more productive than wild-type. Thus, the down-regulation of NPQ appears to be a suitable strategy for improving light use efficiency for biomass and biofuel production in microalgae. PMID:26888481

  20. The potential of C4 grasses for cellulosic biofuel production.

    PubMed

    van der Weijde, Tim; Alvim Kamei, Claire L; Torres, Andres F; Vermerris, Wilfred; Dolstra, Oene; Visser, Richard G F; Trindade, Luisa M

    2013-01-01

    With the advent of biorefinery technologies enabling plant biomass to be processed into biofuel, many researchers set out to study and improve candidate biomass crops. Many of these candidates are C4 grasses, characterized by a high productivity and resource use efficiency. In this review the potential of five C4 grasses as lignocellulosic feedstock for biofuel production is discussed. These include three important field crops-maize, sugarcane and sorghum-and two undomesticated perennial energy grasses-miscanthus and switchgrass. Although all these grasses are high yielding, they produce different products. While miscanthus and switchgrass are exploited exclusively for lignocellulosic biomass, maize, sorghum, and sugarcane are dual-purpose crops. It is unlikely that all the prerequisites for the sustainable and economic production of biomass for a global cellulosic biofuel industry will be fulfilled by a single crop. High and stable yields of lignocellulose are required in diverse environments worldwide, to sustain a year-round production of biofuel. A high resource use efficiency is indispensable to allow cultivation with minimal inputs of nutrients and water and the exploitation of marginal soils for biomass production. Finally, the lignocellulose composition of the feedstock should be optimized to allow its efficient conversion into biofuel and other by-products. Breeding for these objectives should encompass diverse crops, to meet the demands of local biorefineries and provide adaptability to different environments. Collectively, these C4 grasses are likely to play a central role in the supply of lignocellulose for the cellulosic ethanol industry. Moreover, as these species are evolutionary closely related, advances in each of these crops will expedite improvements in the other crops. This review aims to provide an overview of their potential, prospects and research needs as lignocellulose feedstocks for the commercial production of biofuel. PMID:23653628

  1. The potential of C4 grasses for cellulosic biofuel production

    PubMed Central

    van der Weijde, Tim; Alvim Kamei, Claire L.; Torres, Andres F.; Vermerris, Wilfred; Dolstra, Oene; Visser, Richard G. F.; Trindade, Luisa M.

    2013-01-01

    With the advent of biorefinery technologies enabling plant biomass to be processed into biofuel, many researchers set out to study and improve candidate biomass crops. Many of these candidates are C4 grasses, characterized by a high productivity and resource use efficiency. In this review the potential of five C4 grasses as lignocellulosic feedstock for biofuel production is discussed. These include three important field crops—maize, sugarcane and sorghum—and two undomesticated perennial energy grasses—miscanthus and switchgrass. Although all these grasses are high yielding, they produce different products. While miscanthus and switchgrass are exploited exclusively for lignocellulosic biomass, maize, sorghum, and sugarcane are dual-purpose crops. It is unlikely that all the prerequisites for the sustainable and economic production of biomass for a global cellulosic biofuel industry will be fulfilled by a single crop. High and stable yields of lignocellulose are required in diverse environments worldwide, to sustain a year-round production of biofuel. A high resource use efficiency is indispensable to allow cultivation with minimal inputs of nutrients and water and the exploitation of marginal soils for biomass production. Finally, the lignocellulose composition of the feedstock should be optimized to allow its efficient conversion into biofuel and other by-products. Breeding for these objectives should encompass diverse crops, to meet the demands of local biorefineries and provide adaptability to different environments. Collectively, these C4 grasses are likely to play a central role in the supply of lignocellulose for the cellulosic ethanol industry. Moreover, as these species are evolutionary closely related, advances in each of these crops will expedite improvements in the other crops. This review aims to provide an overview of their potential, prospects and research needs as lignocellulose feedstocks for the commercial production of biofuel. PMID:23653628

  2. THE PRODUCTION OF SYNGAS VIA HIGH TEMPERATURE ELECTROLYSIS AND BIO-MASS GASIFICATION

    SciTech Connect

    M. G. McKellar; G. L. Hawkes; J. E. O'Brien

    2008-11-01

    A process model of syngas production using high temperature electrolysis and biomass gasification is presented. Process heat from the biomass gasifier is used to improve the hydrogen production efficiency of the steam electrolysis process. Hydrogen from electrolysis allows a high utilization of the biomass carbon for syngas production. Based on the gasifier temperature, 94% to 95% of the carbon in the biomass becomes carbon monoxide in the syngas (carbon dioxide and hydrogen). Assuming the thermal efficiency of the power cycle for electricity generation is 50%, (as expected from GEN IV nuclear reactors), the syngas production efficiency ranges from 70% to 73% as the gasifier temperature decreases from 1900 K to 1500 K.

  3. Biogas Production from Vietnamese Animal Manure, Plant Residues and Organic Waste: Influence of Biomass Composition on Methane Yield

    PubMed Central

    Cu, T. T. T.; Nguyen, T. X.; Triolo, J. M.; Pedersen, L.; Le, V. D.; Le, P. D.; Sommer, S. G.

    2015-01-01

    Anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. In developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. This study was therefore carried out with the objective of estimating the biogas production potential of typical Vietnamese biomasses such as animal manure, slaughterhouse waste and plant residues, and developing a model that relates methane (CH4) production to the chemical characteristics of the biomass. The biochemical methane potential (BMP) and biomass characteristics were measured. Results showed that piglet manure produced the highest CH4 yield of 443 normal litter (NL) CH4 kg−1 volatile solids (VS) compared to 222 from cows, 177 from sows, 172 from rabbits, 169 from goats and 153 from buffaloes. Methane production from duckweed (Spirodela polyrrhiza) was higher than from lawn grass and water spinach at 340, 220, and 110.6 NL CH4 kg−1 VS, respectively. The BMP experiment also demonstrated that the CH4 production was inhibited with chicken manure, slaughterhouse waste, cassava residue and shoe-making waste. Statistical analysis showed that lipid and lignin are the most significant predictors of BMP. The model was developed from knowledge that the BMP was related to biomass content of lipid, lignin and protein from manure and plant residues as a percentage of VS with coefficient of determination (R-square) at 0.95. This model was applied to calculate the CH4 yield for a household with 17 fattening pigs in the highlands and lowlands of northern Vietnam. PMID:25557826

  4. Biogas production from vietnamese animal manure, plant residues and organic waste: influence of biomass composition on methane yield.

    PubMed

    Cu, T T T; Nguyen, T X; Triolo, J M; Pedersen, L; Le, V D; Le, P D; Sommer, S G

    2015-02-01

    Anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. In developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. This study was therefore carried out with the objective of estimating the biogas production potential of typical Vietnamese biomasses such as animal manure, slaughterhouse waste and plant residues, and developing a model that relates methane (CH4) production to the chemical characteristics of the biomass. The biochemical methane potential (BMP) and biomass characteristics were measured. Results showed that piglet manure produced the highest CH4 yield of 443 normal litter (NL) CH4 kg(-1) volatile solids (VS) compared to 222 from cows, 177 from sows, 172 from rabbits, 169 from goats and 153 from buffaloes. Methane production from duckweed (Spirodela polyrrhiza) was higher than from lawn grass and water spinach at 340, 220, and 110.6 NL CH4 kg(-1) VS, respectively. The BMP experiment also demonstrated that the CH4 production was inhibited with chicken manure, slaughterhouse waste, cassava residue and shoe-making waste. Statistical analysis showed that lipid and lignin are the most significant predictors of BMP. The model was developed from knowledge that the BMP was related to biomass content of lipid, lignin and protein from manure and plant residues as a percentage of VS with coefficient of determination (R-square) at 0.95. This model was applied to calculate the CH4 yield for a household with 17 fattening pigs in the highlands and lowlands of northern Vietnam. PMID:25557826

  5. Seasonal evolution of Biomass Production Efficiency (BPE) of a French beech forest.

    NASA Astrophysics Data System (ADS)

    Heid, L.; Calvaruso, C.; Conil, S.; Turpault, M. P.; Longdoz, B.

    2015-12-01

    With the evolution of ecosystem management and the actual climate change we are facing, there is a need to improve our knowledge of carbon (C) balance and more specifically of C allocation in the plants. In our study, we quantified the seasonal variation of gross primary production (GPP, obtained through eddy covariance measurements) and biomass production (BP, the C fixed into the biomass obtained thanks to inventory campaign) for a 60-year-old even-aged beech stand located in North East of France. We also assessed the seasonal evolution of the BP efficiency (BPE=BP/GPP; Vicca et al., 2012) and its potential determining factors for our site. For 2014, we found a net ecosystem exchange (NEE) of -549 gC m-2, corresponding to a C sequestration. This value breaks down between 1089 gC m-2 for the respiration of the ecosystem and -1639 gC m-2 for the GPP. On the same year, our stand built up 461.6 gC m-2 of tree biomass (leaves, trunk, branches, fine roots), leading to an annual BPE of 0.28, which is within the range of value found on other similar sites. There was a large temporal variation of C allocation to the different parts of the tree biomass during the growth season. Our results show that the growth first happened in the trunk and branches -with a peak value of 74.5 gC m-2 month-1 in May - whereas the fine roots biomass production started later (end of July) and reached a maximum at the end of the growth season (28.49 gC m-2 month-1 for September). The BPE varied also during the year from 0.13 in April to 0.31 in August, where the BP was the same than in July but the cumulated GPP was already decreasing. The seasonal variation may be mainly explained by climatic variations, whereas the shift between woody above-ground biomass and fine roots biomass could be explained by the phenology (linked to physiological mechanisms).

  6. Water- and radiation-use efficiency models for estimating biomass production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Simple biomass production models are used to assess crop productivity under a wide range of environmental conditions, typically as a component of comprehensive crop growth models. Two simple biomass production models are widely used, one based on radiation-use efficiency, e (B=e St fi), and the oth...

  7. The place of algae in agriculture: policies for algal biomass production.

    PubMed

    Trentacoste, Emily M; Martinez, Alice M; Zenk, Tim

    2015-03-01

    Algae have been used for food and nutraceuticals for thousands of years, and the large-scale cultivation of algae, or algaculture, has existed for over half a century. More recently algae have been identified and developed as renewable fuel sources, and the cultivation of algal biomass for various products is transitioning to commercial-scale systems. It is crucial during this period that institutional frameworks (i.e., policies) support and promote development and commercialization and anticipate and stimulate the evolution of the algal biomass industry as a source of renewable fuels, high value protein and carbohydrates and low-cost drugs. Large-scale cultivation of algae merges the fundamental aspects of traditional agricultural farming and aquaculture. Despite this overlap, algaculture has not yet been afforded a position within agriculture or the benefits associated with it. Various federal and state agricultural support and assistance programs are currently appropriated for crops, but their extension to algal biomass is uncertain. These programs are essential for nascent industries to encourage investment, build infrastructure, disseminate technical experience and information, and create markets. This review describes the potential agricultural policies and programs that could support algal biomass cultivation, and the barriers to the expansion of these programs to algae. PMID:24599393

  8. Switchgrass biomass to ethanol production economics: Field to fuel approach

    NASA Astrophysics Data System (ADS)

    Haque, Mohua

    Scope and Method of Study. Switchgrass has been proposed as a dedicated energy crop. The first essay determines switchgrass yield response to nitrogen fertilizer for a single annual harvest in July and for a single annual harvest in October based on a field experiments conducted at Stillwater, OK. Data were fitted to several functional forms to characterize both the July harvest and the October harvest response functions. Extending the harvest window to take advantage of reduction in harvest machinery investment costs has important biological consequences. The second essay determines the cost to deliver a ton of switchgrass biomass to a 2,000 tons per day plant located in Oklahoma. The model accounts for differences in yield and nitrogen fertilizer requirements across harvest months. The data were incorporated into a multi-region, multi-period, monthly time-step, mixed integer mathematical programming model that was constructed to determine the optimal strategy. Desirable feedstock properties, biomass to biofuel conversion rate, and investment required in plant differs depending on which conversion technology is used. The third essay determines the breakeven ethanol price for a cellulosic biorefinery. A comprehensive mathematical programming model that encompasses the chain from land acquisition to ethanol production was constructed and solved. Findings and Conclusions. The July and October harvest plateau yield of 4.36 and 5.49 tons per acre were achieved with an estimated annual nitrogen fertilizer application of 80 and 63 pounds per acre, respectively. Farm gate production costs were estimated to be 60 per ton for the July harvest and 50 per ton for the October harvest. Based on the model results, the strategy of extending harvest over many months is economically preferable to a strategy of harvesting only in peak yield harvest months. Restricting harvest to a two-month harvest season would increase the cost to deliver feedstock by 23 percent. For a capital

  9. The cost of ethanol production from lignocellulosic biomass -- A comparison of selected alternative processes. Final report

    SciTech Connect

    Grethlein, H.E.; Dill, T.

    1993-04-30

    The purpose of this report is to compare the cost of selected alternative processes for the conversion of lignocellulosic biomass to ethanol. In turn, this information will be used by the ARS/USDA to guide the management of research and development programs in biomass conversion. The report will identify where the cost leverages are for the selected alternatives and what performance parameters need to be achieved to improve the economics. The process alternatives considered here are not exhaustive, but are selected on the basis of having a reasonable potential in improving the economics of producing ethanol from biomass. When other alternatives come under consideration, they should be evaluated by the same methodology used in this report to give fair comparisons of opportunities. A generic plant design is developed for an annual production of 25 million gallons of anhydrous ethanol using corn stover as the model substrate at $30/dry ton. Standard chemical engineering techniques are used to give first order estimates of the capital and operating costs. Following the format of the corn to ethanol plant, there are nine sections to the plant; feed preparation, pretreatment, hydrolysis, fermentation, distillation and dehydration, stillage evaporation, storage and denaturation, utilities, and enzyme production. There are three pretreatment alternatives considered: the AFEX process, the modified AFEX process (which is abbreviated as MAFEX), and the STAKETECH process. These all use enzymatic hydrolysis and so an enzyme production section is included in the plant. The STAKETECH is the only commercially available process among the alternative processes.

  10. Optimization of simultaneous biomass production and nutrient removal by mixotrophic Chlorella sp. using response surface methodology.

    PubMed

    Lee, Yu-Ru; Chen, Jen-Jeng

    2016-01-01

    The bioprospecting of potentially mixotrophic microalgae in a constructed wetland was conducted. A locally isolated microalga, Chlorella sp., was grown to determine the effect of temperature, aeration rate, and cultivation time on simultaneous biomass production and nutrient removal from piggery wastewater using central composite design (CCD). The most important variable for the biomass productivity of Chlorella sp. was aeration rate, while that for lipid content and nutrient removal efficiency was cultivation time. Total nitrogen (TN) and total phosphorus (TP) removal efficiencies were higher than that of chemical oxygen demand (COD) from piggery wastewater. The CCD results indicate that the highest biomass productivity (79.2 mg L(-1) d(-1)) and simultaneous nutrient removal efficiency (TN 80.9%, TP 99.2%, COD 74.5%) were obtained with a cultivation temperature of 25 °C, a cultivation time of 5 days, and an air aeration rate of 1.6 L L(-1) min(-1). Palmitic acid (C16:0) and linoleic acid (C18:2) were both abundant in Chlorella sp. cells under mixotrophic cultivation with piggery wastewater. PMID:27054723

  11. Production of 15N-depleted biomass during cyanobacterial N2-fixation at high Fe concentrations

    NASA Astrophysics Data System (ADS)

    Zerkle, Aubrey L.; Junium, Christopher K.; Canfield, Donald E.; House, Christopher H.

    2008-09-01

    In this study we examine the effects of varying Fe, Mo, and P concentrations on δ15N fractionation during N2 fixation in the cyanobacterium Anabaena variabilis. We show that when grown in Fe-enriched media ([Fe] ≥ 50 nM), this organism produces biomass up to 3‰ lower in δ15N than when grown in Fe-limited media ([Fe] < 50 nM). A compilation of our data with previous measurements of δ15N in N2-fixing cyanobacteria reveals a general trend toward the production of more 15N-depleted biomass at higher Fe concentrations. We discuss our results in the context of negative δ15N values preserved in Archean and some Phanerozoic sediments, generally attributed to the production of marine organic matter with low δ15N by N2 fixation (and potentially NH4+ regeneration) during periods of fluctuating nutrient dynamics. We suggest that enhanced Fe availability during periods of widespread ocean anoxia can further stimulate the production of 15N-depleted biomass by N2-fixing organisms, contributing to the isotopic record.

  12. Marker-Trait Association for Biomass Yield of Potential Bio-fuel Feedstock Miscanthus sinensis from Southwest China

    PubMed Central

    Nie, Gang; Huang, Linkai; Zhang, Xinquan; Taylor, Megan; Jiang, Yiwei; Yu, Xiaoqing; Liu, Xinchun; Wang, Xinyu; Zhang, Yajie

    2016-01-01

    As a great potential bio-fuel feedstock, the genus Miscanthus has been widely studied around the world, especially Miscanthus × giganteus owing to its high biomass yield in Europe and North America. However, the narrow genetic basis and sterile characteristics of M. × giganteus have become a limitation for utilization and adaptation to extreme climate conditions. In this study, we focused on one of the progenitors of M. × giganteus, Miscanthus sinensis, which was originally distributed in East Asia with abundant genetic resources and comparable biomass yield potential to M. × giganteus in some areas. A collection of 138 individuals was selected for conducting a 3-year trial of biomass production and analyzed by using 104 pairs of SRAP, ISAP, and SSR primers for genetic diversity as well as marker-trait association. Significant differences in biomass yield and related traits were observed among individuals. Tiller number, fresh biomass yield per plant and dry biomass yield per plant had a high level of phenotypic variation among individuals and the coefficient of variation were all above 40% in 2011, 2012, and 2013. The majority of the traits had a significant correlation with the biomass yield except for the length and width of flag leaves. Plant height was a highly stable trait correlated with biomass yield. A total of 1059 discernible loci were detected by markers across individuals. The population structure (Q) and cluster analyses identified three subpopulations in the collection and family relative kinship (K) represented high gene flow among M. sinensis populations from Southwest China. Model testing identified that Q+K was the best model for describing the associations between the markers and traits, compared to the simple linear, Q or K model. Using the Q+K model, 12 significant associations (P < 0.001) were identified including four markers with plant height and one with biomass yield. Such associations would serve an efficient tool for an early

  13. Marker-Trait Association for Biomass Yield of Potential Bio-fuel Feedstock Miscanthus sinensis from Southwest China.

    PubMed

    Nie, Gang; Huang, Linkai; Zhang, Xinquan; Taylor, Megan; Jiang, Yiwei; Yu, Xiaoqing; Liu, Xinchun; Wang, Xinyu; Zhang, Yajie

    2016-01-01

    As a great potential bio-fuel feedstock, the genus Miscanthus has been widely studied around the world, especially Miscanthus × giganteus owing to its high biomass yield in Europe and North America. However, the narrow genetic basis and sterile characteristics of M. × giganteus have become a limitation for utilization and adaptation to extreme climate conditions. In this study, we focused on one of the progenitors of M. × giganteus, Miscanthus sinensis, which was originally distributed in East Asia with abundant genetic resources and comparable biomass yield potential to M. × giganteus in some areas. A collection of 138 individuals was selected for conducting a 3-year trial of biomass production and analyzed by using 104 pairs of SRAP, ISAP, and SSR primers for genetic diversity as well as marker-trait association. Significant differences in biomass yield and related traits were observed among individuals. Tiller number, fresh biomass yield per plant and dry biomass yield per plant had a high level of phenotypic variation among individuals and the coefficient of variation were all above 40% in 2011, 2012, and 2013. The majority of the traits had a significant correlation with the biomass yield except for the length and width of flag leaves. Plant height was a highly stable trait correlated with biomass yield. A total of 1059 discernible loci were detected by markers across individuals. The population structure (Q) and cluster analyses identified three subpopulations in the collection and family relative kinship (K) represented high gene flow among M. sinensis populations from Southwest China. Model testing identified that Q+K was the best model for describing the associations between the markers and traits, compared to the simple linear, Q or K model. Using the Q+K model, 12 significant associations (P < 0.001) were identified including four markers with plant height and one with biomass yield. Such associations would serve an efficient tool for an early

  14. Nutrient recovery from swine waste and protein biomass production using duckweed ponds (Landoltia punctata): southern Brazil.

    PubMed

    Mohedano, R A; Velho, V F; Costa, R H R; Hofmann, S M; Belli Filho, P

    2012-01-01

    Brazil is one of the most important countries in pork production worldwide, ranking third. This activity has an important role in the national economic scenario. However, the fast growth of this activity has caused major environmental impacts, especially in developing countries. The large amount of nitrogen and phosphorus compounds found in pig manure has caused ecological imbalances, with eutrophication of major river basins in the producing regions. Moreover, much of the pig production in developing countries occurs on small farms, and therefore causes diffuse pollution. Therefore, duckweed pond have been successfully used in the swine waste polishing, generating further a biomass with high protein content. The present study evaluated the efficiency of two full scale duckweed ponds for the polishing of a small pig farm effluent, biomass yield and crude protein (CP) content. Duckweed pond series received the effluent from a biodigester-storage pond, with a flow rate of 1 m(3)/day (chemical oxygen demand rate = 186 kg/ha day) produced by 300 animals. After 1 year a great improvement of effluent quality was observed, with removal of 96% of total Kjeldahl nitrogen (TKN) and 89% of total phosphorus (TP), on average. Nitrogen removal rate is one of the highest ever found (4.4 g TKN/m(2) day). Also, the dissolved oxygen rose from 0.0 to 3.0 mg/L. The two ponds produced together over 13 tons of fresh biomass (90.5% moisture), with 35% of CP content, which represents a productivity of 24 tonsCP/ha year. Due to the high rate of nutrient removal, and also the high protein biomass production, duckweed ponds revealed, under the presented conditions, a great potential for the polishing and valorization of swine waste. Nevertheless, this technology should be better exploited to improve the sustainability of small pig farms in order to minimize the impacts of this activity on the environment. PMID:22592476

  15. d-lactic acid production from renewable lignocellulosic biomass via genetically modified Lactobacillus plantarum.

    PubMed

    Zhang, Yixing; Kumar, Amit; Hardwidge, Philip R; Tanaka, Tsutomu; Kondo, Akihiko; Vadlani, Praveen V

    2016-03-01

    d-lactic acid is of great interest because of increasing demand for biobased poly-lactic acid (PLA). Blending poly-l-lactic acid with poly-d-lactic acid greatly improves PLA's mechanical and physical properties. Corn stover and sorghum stalks treated with 1% sodium hydroxide were investigated as possible substrates for d-lactic acid production by both sequential saccharification and fermentation and simultaneous saccharification and cofermentation (SSCF). A commercial cellulase (Cellic CTec2) was used for hydrolysis of lignocellulosic biomass and an l-lactate-deficient mutant strain Lactobacillus plantarum NCIMB 8826 ldhL1 and its derivative harboring a xylose assimilation plasmid (ΔldhL1-pCU-PxylAB) were used for fermentation. The SSCF process demonstrated the advantage of avoiding feedback inhibition of released sugars from lignocellulosic biomass, thus significantly improving d-lactic acid yield and productivity. d-lactic acid (27.3 g L(-1) ) and productivity (0.75 g L(-1) h(-1) ) was obtained from corn stover and d-lactic acid (22.0 g L(-1) ) and productivity (0.65 g L(-1) h(-1) ) was obtained from sorghum stalks using ΔldhL1-pCU-PxylAB via the SSCF process. The recombinant strain produced a higher concentration of d-lactic acid than the mutant strain by using the xylose present in lignocellulosic biomass. Our findings demonstrate the potential of using renewable lignocellulosic biomass as an alternative to conventional feedstocks with metabolically engineered lactic acid bacteria to produce d-lactic acid. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:271-278, 2016. PMID:26700935

  16. Potential and challenges of zeolite chemistry in the catalytic conversion of biomass.

    PubMed

    Ennaert, Thijs; Van Aelst, Joost; Dijkmans, Jan; De Clercq, Rik; Schutyser, Wouter; Dusselier, Michiel; Verboekend, Danny; Sels, Bert F

    2016-02-01

    Increasing demand for sustainable chemicals and fuels has pushed academia and industry to search for alternative feedstocks replacing crude oil in traditional refineries. As a result, an immense academic attention has focused on the valorisation of biomass (components) and derived intermediates to generate valuable platform chemicals and fuels. Zeolite catalysis plays a distinct role in many of these biomass conversion routes. This contribution emphasizes the progress and potential in zeolite catalysed biomass conversions and relates these to concepts established in existing petrochemical processes. The application of zeolites, equipped with a variety of active sites, in Brønsted acid, Lewis acid, or multifunctional catalysed reactions is discussed and generalised to provide a comprehensive overview. In addition, the feedstock shift from crude oil to biomass involves new challenges in developing fields, like mesoporosity and pore interconnectivity of zeolites and stability of zeolites in liquid phase. Finally, the future challenges and perspectives of zeolites in the processing of biomass conversion are discussed. PMID:26691750

  17. Hydrogen production from high moisture content biomass in supercritical water

    SciTech Connect

    Antal, M.J. Jr.; Xu, X.

    1998-08-01

    By mixing wood sawdust with a corn starch gel, a viscous paste can be produced that is easily delivered to a supercritical flow reactor by means of a cement pump. Mixtures of about 10 wt% wood sawdust with 3.65 wt% starch are employed in this work, which the authors estimate to cost about $0.043 per lb. Significant reductions in feed cost can be achieved by increasing the wood sawdust loading, but such an increase may require a more complex pump. When this feed is rapidly heated in a tubular flow reactor at pressures above the critical pressure of water (22 MPa), the sawdust paste vaporizes without the formation of char. A packed bed of carbon catalyst in the reactor operating at about 650 C causes the tarry vapors to react with water, producing hydrogen, carbon dioxide, and some methane with a trace of carbon monoxide. The temperature and history of the reactor`s wall influence the hydrogen-methane product equilibrium by catalyzing the methane steam reforming reaction. The water effluent from the reactor is clean. Other biomass feedstocks, such as the waste product of biodiesel production, behave similarly. Unfortunately, sewage sludge does not evidence favorable gasification characteristics and is not a promising feedstock for supercritical water gasification.

  18. Use of mechanical refining to improve the production of low-cost sugars from lignocellulosic biomass.

    PubMed

    Park, Junyeong; Jones, Brandon; Koo, Bonwook; Chen, Xiaowen; Tucker, Melvin; Yu, Ju-Hyun; Pschorn, Thomas; Venditti, Richard; Park, Sunkyu

    2016-01-01

    Mechanical refining is widely used in the pulp and paper industry to enhance the end-use properties of products by creating external fibrillation and internal delamination. This technology can be directly applied to biochemical conversion processes. By implementing mechanical refining technology, biomass recalcitrance to enzyme hydrolysis can be overcome and carbohydrate conversion can be enhanced with commercially attractive levels of enzymes. In addition, chemical and thermal pretreatment severity can be reduced to achieve the same level of carbohydrate conversion, which reduces pretreatment cost and results in lower concentrations of inhibitors. Refining is versatile and a commercially proven technology that can be operated at process flows of ∼ 1500 dry tons per day of biomass. This paper reviews the utilization of mechanical refining in the pulp and paper industry and summarizes the recent development in applications for biochemical conversion, which potentially make an overall biorefinery process more economically viable. PMID:26338276

  19. Integration of microalgal cultivation system for wastewater remediation and sustainable biomass production.

    PubMed

    Gupta, Prabuddha L; Lee, Seung-Mok; Choi, Hee-Jeong

    2016-08-01

    Untreated wastewaters have been a great concern and can cause major pollution problems for environment. Conventional approaches for treating wastewater involve tremendous capital cost, have major short comings and are not sustainable. Microalgae culture offers an interesting step for wastewater treatment. Microalgae serve the dual purpose of phycoremediation along with the production of potentially valuable biomass, which can be used for several purposes. The ability of microalgae to accumulate nitrogen, phosphorus, heavy metals and other toxic compounds can be integrated with wastewater treatment system to offer an elegant solution towards tertiary and quaternary treatment. The current review explores possible role of microalgal based wastewater treatment and explores the current progress, key challenges, limitations and future prospects with special emphasis on strategies involved in harvesting, boosting biomass and lipid yield. PMID:27357407

  20. Biomass, production and woody detritus in an old coast redwood (Sequoia sempervirens) forest

    USGS Publications Warehouse

    Busing, R.T.; Fujimori, T.

    2005-01-01

    We examined aboveground biomass dynamics, aboveground net primary production (ANPP), and woody detritus input in an old Sequoia sempervirens stand over a three-decade period. Our estimates of aboveground biomass ranged from 3300 to 5800 Mg ha-1. Stem biomass estimates ranged from 3000 to 5200 Mg ha-1. Stem biomass declined 7% over the study interval. Biomass dynamics were patchy, with marked declines in recent tree-fall patches <0.05 ha in size. Larger tree-fall patches approaching 0.2 ha in size were observed outside the study plot. Our estimates of ANPP ranged from 6 to 14 Mg ha -1yr-1. Estimates of 7 to 10 Mg ha-1yr -1 were considered to be relatively accurate. Thus, our estimates based on long-term data corroborated the findings of earlier short-term studies. ANPP of old, pure stands of Sequoia was not above average for temperate forests. Even though production was potentially high on a per stem basis, it was moderate at the stand level. We obtained values of 797 m3 ha -1 and 262 Mg ha-1 for coarse woody detritus volume and mass, respectively. Fine woody detritus volume and mass were estimated at 16 m3 ha-1 and 5 Mg ha-1, respectively. Standing dead trees (or snags) comprised 7% of the total coarse detritus volume and 8% of the total mass. Coarse detritus input averaged 5.7 to 6.9 Mg ha -1yr-1. Assuming steady-state input and pool of coarse detritus, we obtained a decay rate constant of 0.022 to 0.026. The old-growth stand of Sequoia studied had extremely high biomass, but ANPP was moderate and the amount of woody detritus was not exceptionally large. Biomass accretion and loss were not rapid in this stand partly because of the slow population dynamics and low canopy turnover rate of Sequoia at the old-growth stage. Nomenclature: Hickman (1993). ?? Springer 2005.

  1. Biomass resource potential for selected crops in Hawaii. [Koa haole (giant leucaena); napier and guinea grass

    SciTech Connect

    Seki, A.

    1982-06-01

    The biomass crops selected for review were koa haole (giant leucaena), napier and guinea grass, and eucalyptus (saligna, grandis, and globulus). The islands examined were Hawaii, Kauai, Maui, and Molokai. The potential land acreage for growing these crops was estimated grossly. As anticipated, the island of Hawaii had the largest land potential with eucalyptus having the greatest potential land acreage.

  2. Potential supply and cost of biomass from energy crops in the TVA region

    SciTech Connect

    Graham, R.L.; Downing, M.E.

    1995-04-01

    The economic and supply structures of energy crop markets have not been established. Establishing the likely price and supply of energy crop biomass in a region is a complex task because biomass is not an established commodity as are oil, natural gas, and coal. In this study, the cost and supply of short-rotation woody crop (SRWC) and switchgrass biomass for the Tennessee Valley Authority (TVA) region-a 276-county area that includes portions of 11 states in the southeastern United States - are projected. Projected prices and quantities of biomass are assumed to be a function of the amount and quality of crop and pasture land available in a region, expected energy crop yields and production costs on differing soils and land types, and the profit that could be obtained from current conventional crop production on these same lands. Results include the supply curves of SRWC and switchgrass biomass that are projected to be available from the entire region, the amount and location of crop and pasture land that would be used, and the conventional agricultural crops that would be displaced as a function of energy crop production. Finally, the results of sensitivity analysis on the projected cost and supply of energy crop biomass are shown. In particular, the separate impacts of varying energy crop production costs and yields, and interest rates are examined.

  3. Effects of iron, manganese, copper, and zinc enrichments on productivity and biomass in the subarctic Pacific

    SciTech Connect

    Coale, K.H. )

    1991-12-01

    Natural plankton populations from subarctic Pacific surface waters were incubated in 7-d experiments with added concentrations of Fe, Mn, Cu, and Zn. Small additions of metals were used to simulate natural perturbations in metal concentrations potentially experienced by marine plankton. Trace metal concentrations, phytoplankton productivity, Chl a, and the species composition of phytoplankton and microzooplankton were measured over the course of the experiment. Although the controls indicated little growth, increases in phytoplankton productivity, Chl a, and cell densities were dramatic after the addition of 0.89 nM Fe, indicating that it may limit the rates of algal production in these waters. Similar increases were observed in experiments with 3.9 nM Cu added. The Cu effect is attributed to a decrease in the grazing activities of the microzooplankton and increases in the rates of production. Mn enrichment had its greatest effect on diatom biomass, whereas Zn enrichment had its greatest effect on other autofluorescent organisms. The extent of trace metal adsorption onto carboy walls was also evaluated. These results imply that natural systems may be affected as follows: natural levels of Fe and Cu may influence phytoplankton productivity and trophic structure in open-ocean, high-nutrient, low-biomass systems; rates of net production are not limited by one micronutrient alone.

  4. Global effects of national biomass production and consumption: Austria's embodied HANPP related to agricultural biomass in the year 2000

    PubMed Central

    Haberl, Helmut; Kastner, Thomas; Schaffartzik, Anke; Ludwiczek, Nikolaus; Erb, Karl-Heinz

    2012-01-01

    Global trade of biomass-related products is growing exponentially, resulting in increasing ‘teleconnections’ between producing and consuming regions. Sustainable management of the earth's lands requires indicators to monitor these connections across regions and scales. The ‘embodied human appropriation of NPP’ (eHANPP) allows one to consistently attribute the HANPP resulting from production chains to consumers. HANPP is the sum of land-use induced NPP changes and biomass harvest. We present the first national-level assessment of embodied HANPP related to agriculture based on a calculation using bilateral trade matrices. The dataset allows (1) the tracing of the biomass-based products consumed in Austria in the year 2000 to their countries of origin and quantifying the HANPP caused in production, and (2) the assigning of the national-level HANPP on Austria's territory to the consumers of the products on the national level. The dataset is constructed along a consistent system boundary between society and ecosystems and can be used to assess Austria's physical trade balance in terms of eHANPP. Austria's eHANPP-trade balance is slightly negative (imports are larger than exports); import and export flows are large in relation to national HANPP. Our findings show how the eHANPP approach can be used for quantifying and mapping the teleconnections related to a nation's biomass metabolism. PMID:23576842

  5. Determination of the potential market size and opportunities for biomass to electricity projects in China

    SciTech Connect

    Perlack, R.D.

    1995-08-01

    Efforts are currently underway to assess the market potential and prospects for the US private sector in biomass energy development in Yunnan Province. Among the specific objectives of the study are to: estimate the likely market size and competitiveness of biomass energy, assess the viability of US private sector ventures; assess non-economic factors (e.g., resource, environmental, social, political, institutional) that could affect the viability of biomass energy; and recommend appropriate actions to help stimulate biomass initiatives. Feasibility studies show that biomass projects in Yunnan Province are financially and technically viable. Biomass can be grown and converted to electricity at costs lower than other alternatives. These projects if implemented can ease power shortages and help to sustain the region`s economic growth. The external environmental benefits of integrated biomass projects are also potentially significant. This paper summarizes a two-step screening and rank-ordering process that is being used to identify the best candidate projects for possible US private sector investment. The process uses a set of initial screens to eliminate projects that are not technically feasible to develop. The remaining projects are then rank-ordered using a multicriteria technique.

  6. Vancomycin production is enhanced in chemostat culture with biomass-recycle.

    PubMed

    McIntyre, J J; Bunch, A W; Bull, A T

    1999-03-01

    Production of the glycopeptide antibiotic vancomycin by Amycolatopsis orientalis ATCC 19795 was examined in phosphate-limited chemostat cultures with biomass-recycle, employing an oscillating membrane separator, at a constant dilution rate (D= 0. 14 h-1). Experiments made under low agitation conditions (600 rpm) showed that the biomass concentration could be increased 3.9-fold with vancomycin production kinetics very similar to that of chemostat culture without biomass-recycle. The specific production rate (qvancomycin) was maximal when the biomass-recycle ratio (R) was 0.13 (D= 0.087 h-1). When the dissolved oxygen tension dropped below 20% (air saturation), the biomass and vancomycin concentrations decreased and an unidentified red metabolite was released into the culture medium. Using increased agitation (850 rpm), used to maintain the dissolved oxygen tension above 20% air saturation, maximum increases in biomass concentration (7.9-fold) and vancomcyin production 1.6-fold (0.6 mg/g dry weight/h) were obtained when R was 0.44 (D= 0.056 h -1) compared to chemostat culture without biomass-recycle. Moreover, at this latter recycle ratio the volumetric vancomycin production rate was 14.7 mg/L/h (a 7-fold increase compared to chemostat culture without biomass-recycle). These observations encourage further research on biomass-recycling as a means of optimising the production of antibiotics. PMID:10099566

  7. Submarine canyons: hotspots of benthic biomass and productivity in the deep sea

    PubMed Central

    De Leo, Fabio C.; Smith, Craig R.; Rowden, Ashley A.; Bowden, David A.; Clark, Malcolm R.

    2010-01-01

    Submarine canyons are dramatic and widespread topographic features crossing continental and island margins in all oceans. Canyons can be sites of enhanced organic-matter flux and deposition through entrainment of coastal detrital export, dense shelf-water cascade, channelling of resuspended particulate material and focusing of sediment deposition. Despite their unusual ecological characteristics and global distribution along oceanic continental margins, only scattered information is available about the influence of submarine canyons on deep-sea ecosystem structure and productivity. Here, we show that deep-sea canyons such as the Kaikoura Canyon on the eastern New Zealand margin (42°01′ S, 173°03′ E) can sustain enormous biomasses of infaunal megabenthic invertebrates over large areas. Our reported biomass values are 100-fold higher than those previously reported for deep-sea (non-chemosynthetic) habitats below 500 m in the ocean. We also present evidence from deep-sea-towed camera images that areas in the canyon that have the extraordinary benthic biomass also harbour high abundances of macrourid (rattail) fishes likely to be feeding on the macro- and megabenthos. Bottom-trawl catch data also indicate that the Kaikoura Canyon has dramatically higher abundances of benthic-feeding fishes than adjacent slopes. Our results demonstrate that the Kaikoura Canyon is one of the most productive habitats described so far in the deep sea. A new global inventory suggests there are at least 660 submarine canyons worldwide, approximately 100 of which could be biomass hotspots similar to the Kaikoura Canyon. The importance of such deep-sea canyons as potential hotspots of production and commercial fisheries yields merits substantial further study. PMID:20444722

  8. Submarine canyons: hotspots of benthic biomass and productivity in the deep sea.

    PubMed

    De Leo, Fabio C; Smith, Craig R; Rowden, Ashley A; Bowden, David A; Clark, Malcolm R

    2010-09-22

    Submarine canyons are dramatic and widespread topographic features crossing continental and island margins in all oceans. Canyons can be sites of enhanced organic-matter flux and deposition through entrainment of coastal detrital export, dense shelf-water cascade, channelling of resuspended particulate material and focusing of sediment deposition. Despite their unusual ecological characteristics and global distribution along oceanic continental margins, only scattered information is available about the influence of submarine canyons on deep-sea ecosystem structure and productivity. Here, we show that deep-sea canyons such as the Kaikoura Canyon on the eastern New Zealand margin (42 degrees 01' S, 173 degrees 03' E) can sustain enormous biomasses of infaunal megabenthic invertebrates over large areas. Our reported biomass values are 100-fold higher than those previously reported for deep-sea (non-chemosynthetic) habitats below 500 m in the ocean. We also present evidence from deep-sea-towed camera images that areas in the canyon that have the extraordinary benthic biomass also harbour high abundances of macrourid (rattail) fishes likely to be feeding on the macro- and megabenthos. Bottom-trawl catch data also indicate that the Kaikoura Canyon has dramatically higher abundances of benthic-feeding fishes than adjacent slopes. Our results demonstrate that the Kaikoura Canyon is one of the most productive habitats described so far in the deep sea. A new global inventory suggests there are at least 660 submarine canyons worldwide, approximately 100 of which could be biomass hotspots similar to the Kaikoura Canyon. The importance of such deep-sea canyons as potential hotspots of production and commercial fisheries yields merits substantial further study. PMID:20444722

  9. Potential application of multipolarization SAR for pine-plantation biomass estimation

    NASA Technical Reports Server (NTRS)

    Wu, Shih-Tseng

    1987-01-01

    This paper presents the technique and the potential utility of multipolarization Synthetic Aperture Radar (SAR) data for pine-plantation biomass estimation. Three channels of SAR data, one from the Shuttle Imaging Radar SIR-A and the other two from the aircraft SAR, were acquired over the Baldwin County, Alabama, study area. The SIR-A data were acquired with HH polarization and the aircraft SAR data with VV and VH polarizations. Linear regression techniques are used to estimate the pine-plantation biomass, tree height, and age using 21 test plots. The results indicate that the multipolarization data are highly related to the plantation biomass. The results suggest a potential application of multipolarization SAR for pine-plantation biomass estimation.

  10. Potential biomass yield per phosphorus and lipid accumulation property of seven microalgal species.

    PubMed

    Wu, Yin-Hu; Yu, Yin; Hu, Hong-Ying

    2013-02-01

    The potential biomass yield per phosphorus and lipid/triglyceride (TAG) accumulation properties of seven microalgal species: Scenedesmus sp. LX1, Chlorella ellipsoidea YJ1, Chlorella vuglaris, Chlorella sorokiniana, Chlorella pyrenoidosa, Dunaliella primolecta and Haematococcus pluvialis were investigated. Among the tested species, Scenedesmus sp. LX1 obtained the smallest minimal phosphorus content in cell (Q(0)) and the highest potential biomass yield of 6100kg-biomass/kg-P. After 12-day growth with intracellular phosphorus, Scenedesmus sp. LX1 accumulated about 30% lipid in biomass. Furthermore, the TAGs content per lipid of this strain (58.5%) as well as the lipid and TAGs yield per phosphorus (1800kg-lipid/kg-P and 680kg-TAGs/kg-P, respectively) were all significantly higher than that of any other species investigated in this study. Therefore, the phosphorus consumption to produce 1kg biodiesel using Scenedesmus sp. LX1 as feedstock was lowest among the tested species. PMID:23334016

  11. Screening Prosopis (mesquite) germplasm for biomass production and nitrogen fixation

    SciTech Connect

    Felker, P.; Cannell, G.H.; Clark, P.R.; Osborn, J.F.

    1980-01-01

    The nitrogen-fixing trees of the genus Prosopis (mesquite or algaroba) are well adapted to the semi-arid and often saline regions of the world. These trees may produce firewood or pods for livestock food, they may stabilize sand dunes and they may enrich the soil by production of leaf litter supported by nitrogen fixation. A collection of nearly 500 Prosopis accessions representing North and South American and African germplasm has been established. Seventy of these accessions representing 14 taxa are being grown under field conditions where a 30-fold range in biomass productivity among accessions has been estimated. In a greehouse experiment, 13 Prosopis taxa grew on nitrogen-free medium nodulated, and had a 10-fold difference in nitrogen fixation (acetylene reduction). When Prosopis is propagated by seed the resulting trees are extremely variable in growth rate and presence or absence of thorns. Propagation of 6 Prosopis taxa by stem cuttings has been achieved with low success (1 to 10%) in field-grown plants and with higher success (50 to 100%) with young actively growing greenhouse plants.

  12. Sustainable biomass products development and evaluation, Hamakua project. Final draft report

    SciTech Connect

    1998-05-01

    The PICHTR Sustainable Biomass Energy Program was developed to evaluate the potential to cultivate crops for energy production as an alternative use of lands made available by the closing of large sugar plantations. In particular, the closing of the Hamakua Sugar Company on the island of Hawaii brought a great deal of attention to the future of agriculture in this region and in the state. Many options were proposed. Several promising alternatives had been proposed for cane lands. These included dedicated feedstock supply systems (DFSS) for electrical energy production, cultivation of sugarcane to produce ethanol and related by-products, and the production of feed and crops to support animal agriculture. Implementation of some of the options might require preservation of large tracts of land and maintenance of the sugar mills and sugar infrastructure. An analysis of the technical, financial, and other issues necessary to reach conclusions regarding the optimal use of these lands was required. At the request of the Office of State Planning and Senator Akaka`s office, the Pacific International Center for High Technology Research (PICHTR) established and coordinated a working group composed of state, county, federal, and private sector representatives to identify sustainable energy options for the use of idle sugar lands on the island of Hawaii. The Sustainable Biomass Energy Program`s Hamakua Project was established to complete a comprehensive evaluation of the most viable alternatives and assess the options to grow crops as a source of raw materials for the production of transportation fuel and/or electricity on the island of Hawaii. The motivation for evaluating biomass to energy conversion embraced the considerations that Hawaii`s energy security would be improved by diversifying the fuels used for transportation and reducing dependency on imported fossil fuels. The use of waste products as feedstocks could divert wastes from landfills.

  13. Co-pyrolysis of low rank coals and biomass: Product distributions

    SciTech Connect

    Soncini, Ryan M; Means, Nicholas C; Weiland, Nathan T

    2013-10-01

    Pyrolysis and gasification of combined low rank coal and biomass feeds are the subject of much study in an effort to mitigate the production of green house gases from integrated gasification combined cycle (IGCC) systems. While co-feeding has the potential to reduce the net carbon footprint of commercial gasification operations, the effects of co-feeding on kinetics and product distributions requires study to ensure the success of this strategy. Southern yellow pine was pyrolyzed in a semi-batch type drop tube reactor with either Powder River Basin sub-bituminous coal or Mississippi lignite at several temperatures and feed ratios. Product gas composition of expected primary constituents (CO, CO{sub 2}, CH{sub 4}, H{sub 2}, H{sub 2}O, and C{sub 2}H{sub 4}) was determined by in-situ mass spectrometry while minor gaseous constituents were determined using a GC-MS. Product distributions are fit to linear functions of temperature, and quadratic functions of biomass fraction, for use in computational co-pyrolysis simulations. The results are shown to yield significant nonlinearities, particularly at higher temperatures and for lower ranked coals. The co-pyrolysis product distributions evolve more tar, and less char, CH{sub 4}, and C{sub 2}H{sub 4}, than an additive pyrolysis process would suggest. For lignite co-pyrolysis, CO and H{sub 2} production are also reduced. The data suggests that evolution of hydrogen from rapid pyrolysis of biomass prevents the crosslinking of fragmented aromatic structures during coal pyrolysis to produce tar, rather than secondary char and light gases. Finally, it is shown that, for the two coal types tested, co-pyrolysis synergies are more significant as coal rank decreases, likely because the initial structure in these coals contains larger pores and smaller clusters of aromatic structures which are more readily retained as tar in rapid co-pyrolysis.

  14. Production of Butyric Acid and Butanol from Biomass

    SciTech Connect

    David E. Ramey; Shang-Tian Yang

    2005-08-25

    Environmental Energy Inc has shown that BUTANOL REPLACES GASOLINE - 100 pct and has no pollution problems, and further proved it is possible to produce 2.5 gallons of butanol per bushel corn at a production cost of less than $1.00 per gallon. There are 25 pct more Btu-s available and an additional 17 pct more from hydrogen given off, from the same corn when making butanol instead of ethanol that is 42 pct more Btu-s more energy out than it takes to make - that is the plow to tire equation is positive for butanol. Butanol is far safer to handle than gasoline or ethanol. Butanol when substituted for gasoline gives better gas mileage and does not pollute as attested to in 10 states. Butanol should now receive the same recognition as a fuel alcohol in U.S. legislation as ethanol. There are many benefits to this technology in that Butanol replaces gasoline gallon for gallon as demonstrated in a 10,000 miles trip across the United States July-August 2005. No modifications at all were made to a 1992 Buick Park Avenue; essentially your family car can go down the road on Butanol today with no modifications, Butanol replaces gasoline. It is that simple. Since Butanol replaces gasoline more Butanol needs to be made. There are many small farms across America which can grow energy crops and they can easily apply this technology. There is also an abundance of plant biomass present as low-value agricultural commodities or processing wastes requiring proper disposal to avoid pollution problems. One example is in the corn refinery industry with 10 million metric tons of corn byproducts that pose significant environmental problems. Whey lactose presents another waste management problem, 123,000 metric tons US, which can now be turned into automobile fuel. The fibrous bed bioreactor - FBB - with cells immobilized in the fibrous matrix packed in the reactor has been successfully used for several organic acid fermentations, including butyric and propionic acids with greatly increased

  15. Key issues in life cycle assessment of ethanol production from lignocellulosic biomass: Challenges and perspectives.

    PubMed

    Singh, Anoop; Pant, Deepak; Korres, Nicholas E; Nizami, Abdul-Sattar; Prasad, Shiv; Murphy, Jerry D

    2010-07-01

    Progressive depletion of conventional fossil fuels with increasing energy consumption and greenhouse gas (GHG) emissions have led to a move towards renewable and sustainable energy sources. Lignocellulosic biomass is available in massive quantities and provides enormous potential for bioethanol production. However, to ascertain optimal biofuel strategies, it is necessary to take into account environmental impacts from cradle to grave. Life cycle assessment (LCA) techniques allow detailed analysis of material and energy fluxes on regional and global scales. This includes indirect inputs to the production process and associated wastes and emissions, and the downstream fate of products in the future. At the same time if not used properly, LCA can lead to incorrect and inappropriate actions on the part of industry and/or policy makers. This paper aims to list key issues for quantifying the use of resources and releases to the environment associated with the entire life cycle of lignocellulosic bioethanol production. PMID:20015644

  16. Bioethanol production from Scenedesmus obliquus sugars: the influence of photobioreactors and culture conditions on biomass production.

    PubMed

    Miranda, J R; Passarinho, P C; Gouveia, L

    2012-10-01

    A closed-loop vertical tubular photobioreactor (PBR), specially designed to operate under conditions of scarce flat land availability and irregular solar irradiance conditions, was used to study the potential of Scenedesmus obliquus biomass/sugar production. The results obtained were compared to those from an open-raceway pond and a closed-bubble column. The influence of the type of light source and the regime (natural vs artificial and continuous vs light/dark cycles) on the growth of the microalga and the extent of the sugar accumulation was studied in both PBRs. The best type of reactor studied was a closed-loop PBR illuminated with natural light/dark cycles. In all the cases, the relationship between the nitrate depletion and the sugar accumulation was observed. The microalga Scenedesmus was cultivated for 53 days in a raceway pond (4,500 L) and accumulated a maximum sugar content of 29 % g/g. It was pre-treated for carrying out ethanol fermentation assays, and the highest ethanol concentration obtained in the hydrolysate fermented by Kluyveromyces marxianus was 11.7 g/L. PMID:22899495

  17. Biogas energy production from tropical biomass wastes by anaerobic digestion

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Anaerobic digestion (AD) is an attractive technology in tropical regions for converting locally abundant biomass wastes into biogas which can be used to produce heat, electricity, and transportation fuels. However, investigations on AD of tropical forestry wastes, such as albizia biomass, and food w...

  18. Remote sensing investigations of wetland biomass and productivity for global biosystems research

    NASA Technical Reports Server (NTRS)

    Klemas, V.

    1986-01-01

    The relationship between spectral radiance and plant canopy biomass was studied in wetlands. Spectroradiometer data was gathered on Thematic Mapper wavebands 3, 4, and 5, and correlated with canopy and edaphic factors determined by harvesting. The relationship between spectral radiance and plant canopy biomass for major salt and brackish canopy types was determined. Algorithms were developed for biomass measurement in mangrove swamps. The influence of latitudinal variability in canopy structure on biomass assessment of selected plants was investigated. Brackish marsh biomass estimates were obtained from low altitude aircraft and compared with ground measurements. Annual net aerial primary productivity estimates computed from spectral radiance data were compiled for a Spartina alterniflora marsh. Spectral radiance data were expressed as vegetation or infrared index values. Biomass estimates computed from models were in close agreement with biomass estimates determined from harvests.

  19. Biomass production and water use of Black Locust (Robinia pseudoacacia L.) for short-rotation plantation

    NASA Astrophysics Data System (ADS)

    Mantovani, D.; Veste, M.; Freese, D.

    2012-04-01

    The early successional tree species Robinia pseudoacacia L. demonstrates a high potential for biomass production in short rotation plantations and agroforestry systems. On marginal lands and recultivated areas, often characterized by poor edaphic conditions, black locust is already successfully cropped. In southern Brandenburg (East Germany), vast areas have been exploited for lignite open cast mining and the outcome is a drastic alteration of the top soil layer and subsurface geological structure, causing a radical change of the hydrologic cycle. Soil poor in nutrient and carbon, combined with low rainfall, limits the reclamation of these areas and their use for conventional agriculture. However, promising results have been obtained by the establishment of black locust for bioenergy production. For the evaluation of the black locust growth potential in southern Brandenburg with its sandy soils and low annual mean rainfall, detailed information about the link between growth, transpiration and soil water availability are needed. Therefore, we determined the biomass-transpiration relation and formulated the equation that describes the intertwined interaction between water use and biomass production. The equation will be integrated into mathematical tools. To reduce the numerous environmental variables involved in field experiments, we grew black locust under semi-controlled environmental conditions by using wick lysimeters. The lysimeters were filled with sandy loam soil and water was supplied solely by an automatic irrigation system in relation to the volumetric soil water content (7%, 10%, and 14%). Rainfall is excluded by a light transmissive roof. Water use efficiency (WUE) at whole plant level is evaluated by the ratio between the biomass produced during the vegetation period and the cumulative daily water use. The study encompasses ecophysiological investigations of the gas exchange (H2O and CO2) on single leaves, to evaluate the influence of the stomata

  20. Influence of precursors and inhibitor on the production of extracellular 5-aminolevulinic acid and biomass by Rhodopseudomonas palustris KG31.

    PubMed

    Saikeur, Angkana; Choorit, Wanna; Prasertsan, Poonsuk; Kantachote, Duangporn; Sasaki, Ken

    2009-05-01

    5-Aminolevulinic acid (ALA) and the biomass of photosynthetic bacteria, Rhodopseudomonas palustris KG31, have very high potential for development and exploitation as bioherbicide and biofertilizer respectively. In this work, the effects of two precursors and an inhibitor of aminolevulinic dehydratase (ALAD) added to the VFA culture medium on the production of ALA and biomass were investigated. The experimental runs were carried out according to a Box-Behnken design. The precursors were added to the medium at the beginning of cultivation, while the inhibitor was added after 24 h. Statistical analysis indicated that levulinic acid (LA) has a positive effect on ALA production while glycine has a negative effect on biomass production. In order to enhance both ALA and biomass products, the most suitable medium was VFA medium supplemented with 3.0 mM glycine and 10 mM LA, giving ALA and biomass of 182.91 microM and 3.1 gDCW/l within 54 h. PMID:19420716

  1. Electrocatalytic processing of renewable biomass-derived compounds for production of chemicals, fuels and electricity

    NASA Astrophysics Data System (ADS)

    Xin, Le

    The dual problems of sustaining the fast growth of human society and preserving the environment for future generations urge us to shift our focus from exploiting fossil oils to researching and developing more affordable, reliable and clean energy sources. Human beings had a long history that depended on meeting our energy demands with plant biomass, and the modern biorefinery technologies realize the effective conversion of biomass to production of transportation fuels, bulk and fine chemicals so to alleviate our reliance on fossil fuel resources of declining supply. With the aim of replacing as much non-renewable carbon from fossil oils with renewable carbon from biomass as possible, innovative R&D activities must strive to enhance the current biorefinery process and secure our energy future. Much of my Ph.D. research effort is centered on the study of electrocatalytic conversion of biomass-derived compounds to produce value-added chemicals, biofuels and electrical energy on model electrocatalysts in AEM/PEM-based continuous flow electrolysis cell and fuel cell reactors. High electricity generation performance was obtained when glycerol or crude glycerol was employed as fuels in AEMFCs. The study on selective electrocatalytic oxidation of glycerol shows an electrode potential-regulated product distribution where tartronate and mesoxalate can be selectively produced with electrode potential switch. This finding then led to the development of AEMFCs with selective production of valuable tartronate or mesoxalate with high selectivity and yield and cogeneration of electricity. Reaction mechanisms of electrocatalytic oxidation of ethylene glycol and 1,2-propanediol were further elucidated by means of an on-line sample collection technique and DFT modeling. Besides electro-oxidation of biorenewable alcohols to chemicals and electricity, electrocatalytic reduction of keto acids (e.g. levulinic acid) was also studied for upgrading biomass-based feedstock to biofuels while

  2. Biomass feedstock production impact on water resource availability

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bioenergy from renewable sources are a major area of interest and technology development globally. Bioenergy crop production is highly dependent on water. Bioenergy development will require effective allocation and management of water. The objectives of this investigation were to review the potenti...

  3. Biomass logistics analysis for large scale biofuel production: case study of loblolly pine and switchgrass.

    PubMed

    Lu, Xiaoming; Withers, Mitch R; Seifkar, Navid; Field, Randall P; Barrett, Steven R H; Herzog, Howard J

    2015-05-01

    The objective of this study was to assess the costs, energy consumption and greenhouse gas (GHG) emissions throughout the biomass supply chain for large scale biofuel production. Two types of energy crop were considered, switchgrass and loblolly pine, as representative of herbaceous and woody biomass. A biomass logistics model has been developed to estimate the feedstock supply system from biomass production through transportation. Biomass in the form of woodchip, bale and pellet was investigated with road, railway and waterway transportation options. Our analysis indicated that the farm or forest gate cost is lowest for loblolly pine whole tree woodchip at $39.7/dry tonne and highest for switchgrass round bale at $72.3/dry tonne. Switchgrass farm gate GHG emissions is approximately 146kgCO2e/dry tonne, about 4 times higher than loblolly pine. The optimum biomass transportation mode and delivered form are determined by the tradeoff between fixed and variable costs for feedstock shipment. PMID:25710677

  4. Remote sensing of aboveground biomass and annual net aerial primary productivity in tidal wetlands

    SciTech Connect

    Hardisky, M.A.

    1983-01-01

    A technique was investigated for estimating biomass and net aerial primary productivity (NAPP) in Delaware tidal marshes from spectral data, describing marsh vegetation canopies. Spectral radiance data were collected with hand-held radiometers from the ground and from low altitude aircraft. Spectral wavebands corresponding to Landsat 4 thematic mapper bands 3, 4 and 5 and multispectral scanner bands 5 and 7 were employed. Spectral data, expressed as index values, were substituted into simple regression models to nondestructively compute total aboveground biomass. Dead biomass, salt crystals on plant leaves and soil background reflectance, all attenuated the spectral radiance index values. A large spectral contribution from any one of these canopy components caused an underestimate of live biomass. Biomass and annual NAPP of a S. alterniflora dominated salt marsh was estimated by traditional harvesting techniques and from ground-gathered spectral radiance data. The live and dead standing crop biomass estimates computed from spectral data were usually not significantly different from harvest biomass estimates. Spectral estimates of NAPP were usually within 10% of NAPP estimates calculated from harvest data. August live standing crop biomass estimates computed from ground-gathered spectral data for a tidal brackish marsh were generally within 10% of harvest estimates. Live biomass estimates computed from spectral data gathered from a low altitude aircraft were equally similar to harvest biomass estimates. The remote sensing technique holds much promise for rapid and accurate estimates of biomass and NAPP in tidal marshes.

  5. Estimation of potential maximum biomass of trout in Wyoming streams to assist management decisions

    USGS Publications Warehouse

    Hubert, W.A.; Marwitz, T.D.; Gerow, K.G.; Binns, N.A.; Wiley, R.W.

    1996-01-01

    Fishery managers can benefit from knowledge of the potential maximum biomass (PMB) of trout in streams when making decisions on the allocation of resources to improve fisheries. Resources are most likely to he expended on streams with high PMB and with large differences between PMB and currently measured biomass. We developed and tested a model that uses four easily measured habitat variables to estimate PMB (upper 90th percentile of predicted mean bid mass) of trout (Oncorhynchus spp., Salmo trutta, and Salvelinus fontinalis) in Wyoming streams. The habitat variables were proportion of cover, elevation, wetted width, and channel gradient. The PMB model was constructed from data on 166 stream reaches throughout Wyoming and validated on an independent data set of 50 stream reaches. Prediction of PMB in combination with estimation of current biomass and information on habitat quality can provide managers with insight into the extent to which management actions may enhance trout biomass.

  6. A hyperspectral approach to estimating biomass and plant production in a heterogeneous restored temperate peatland

    NASA Astrophysics Data System (ADS)

    Byrd, K. B.; Schile, L. M.; Windham-Myers, L.; Kelly, M.; Hatala, J.; Baldocchi, D. D.

    2012-12-01

    Restoration of drained peatlands that are managed to reverse subsidence through organic accretion holds significant potential for large-scale carbon storage and sequestration. This potential has been demonstrated in an experimental wetland restoration site established by the U.S. Geological Survey in 1997 on Twitchell Island in the Sacramento-San Joaquin River Delta, where soil carbon storage is up to 1 kg C m-2 and root and rhizome production can reach over 7 kg m-2 annually. Remote sensing-based estimation of biomass and productivity over a large spatial extent helps to monitor carbon storage potential of these restored peatlands. Extensive field measurements of plant biophysical characteristics such as biomass, leaf area index, and the fraction of absorbed photosynthetically active radiation (fAPAR) [an important variable in light-use efficiency (LUE) models] have been collected for agricultural systems and forests. However the small size and local spatial variability of U.S. Pacific Coast wetlands pose new challenges for measuring these variables in the field and generating estimates through remote sensing. In particular background effects of non-photosynthetic vegetation (NPV), floating aquatic vegetation, and inundation of wetland vegetation influence the relationship between field measurements and multispectral or hyperspectral indices. Working at the USGS experimental wetland site, characterized by variable water depth and substantial NPV, or thatch, we collected field data on hardstem bulrush (Schoenoplectus acutus) and cattail (Typha spp.) coupled with reflectance data from a field spectrometer (350-2500 nm) every two to three weeks during the summers of 2011 and 2012. We calculated aboveground biomass with existing allometric relationships, and fAPAR was measured with line and point quantum sensors. We analyzed reflectance data to develop hyperspectral and multispectral indices that predict biomass and fAPAR and account for background effects of water

  7. Biomass and carotenoid production in photosynthetic bacteria wastewater treatment: effects of light intensity.

    PubMed

    Zhou, Qin; Zhang, Panyue; Zhang, Guangming

    2014-11-01

    This study investigated the feasibility of using photosynthetic bacteria (PSB) to produce biomass and carotenoid while treating wastewater. The effects of light intensity on the biomass, carotenoid and bacteriochlorophyll accumulation in together with pollutant removal were studied. Results showed that it was feasible to use PSB to treat wastewater as well as to produce biomass or carotenoid. 2000 lux was an optimal intensity for biomass production and COD removal, and the corresponding values were 2645 mg/L and 94.7%. 8000 lux was an optimal light intensity for carotenoid production (1.455 mg/L). Mechanism analysis displayed that the greater the bacteriochlorophyll and carotenoid were secreted, the lower the light conversion efficiency turned out to be. The highest light conversion efficiency was achieved at 500 lux; the ATP production, biomass production, and COD removal were the highest at 2000 lux, but the bacteriochlorophyll and carotenoid content were the lowest at 2000 lux. PMID:25218205

  8. Solar Pond devices: free energy or bioreactors for Artemia biomass production?

    PubMed

    Gouveia, Luisa; Sousa, João; Marques, Ana; Tavares, Célia; Giestas, Margarida

    2009-08-01

    The recent exponential growth in industrial aquaculture has led to a huge increase in Artemia biomass production in order to meet increased fish production needs. The present study explores the potential use of salt gradient solar ponds (SGSPs) for production of Artemia nauplii. An SGSP is a basin of water where solar energy is trapped and collected via an artificially imposed gradient. Three zones can be identified in an SGSP: upper and lower zones, which are both convective, and a middle zone, which is intended to be non-convective. The latter acts as a transparent insulation layer and allows for storage of solar energy at the bottom, where it is available for use. The combination of salt, temperature and high transparency could make SGSPs promising bioreactors for the production of Artemia nauplii. Using particle image velocymetry (PIV) and Shadowgraph visualisation techniques, the behaviour of Artemia nauplii under critical cultivation parameters (namely, salinity, temperature and light) was monitored to determine movement velocity, and how movement of Artemia affects the salt gradient. It was observed that Artemia nauplii constantly follow light, irrespective of adverse salinity and/or temperature conditions. However, despite the substantial displacement of Artemia following the light source, the salt gradient is not disrupted. The suitability of SGSPs as bioreactors for Artemia biomass production was then tested. The results were disappointing, probably due to the lack of sufficient O(2) for Artemia survival and growth. Follow-up trials were conducted aimed at using the SGSP as a green and economically attractive energy source to induce faster hatching of cysts and improved Artemia nauplii growth. The results of these trials, and a case study of Artemia nauplii production using an SGSP, are presented. The authors constructed a Solar Pond device, which they suggest as a novel way of supplying thermal energy for Artemia biomass production in an aquaculture

  9. Potential of Reduction in CO2 Emission by Biomass Power Generation with Thinning Residues

    NASA Astrophysics Data System (ADS)

    Makino, Yosuke; Kato, Takeyoshi; Suzuoki, Yasuo

    In Japan, forest thinning residues as woody biomass have potential to increase domestic primary energy supply, because there still remain many conifer plantations where thinning is not carried out. However, taking the reduction in carbon stock in forests into account, the additional thinning for energy supply may not contribute to the reduction in CO2 emission. Considering the change in the carbon stock in forests, this paper discusses the potential of reduction in CO2 emission by biomass power generation with thinning residues. As power generation systems with thinning residues, co-firing with coal in a utility's power station and a molten carbonate fuel cell (MCFC) with gasification system are taken into account. The results suggest that the co-firing of woody biomass supplied by the additional thinning at utilities' coal-fired power stations has a potential for reducing overall CO2 emission.

  10. Potential of energy production from conserved forages

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Forages have a potential role in meeting the demand for energy. Perennial forages are attractive for various reasons. One, both the monetary and energy cost of planting is spread over many years. Two, we already have the equipment for harvesting, storing and transporting this source of biomass. Thre...

  11. Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae.

    PubMed

    Hara, Kiyotaka Y; Kim, Songhee; Yoshida, Hideyo; Kiriyama, Kentaro; Kondo, Takashi; Okai, Naoko; Ogino, Chiaki; Fukuda, Hideki; Kondo, Akihiko

    2012-02-01

    Glutathione is a valuable tri-peptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is produced industrially by fermentation using Saccharomyces cerevisiae, and supplementation of fermentation with several amino acids can increase intracellular GSH content. More recently, however, focus has been given to protein as a resource for biofuel and fine chemical production. We demonstrate that expression of a protease on the cell surface of S. cerevisiae enables the direct use of keratin and soy protein as a source of amino acids and that these substrates enhanced intracellular GSH content. Furthermore, fermentation using soy protein also enhanced cell concentration. GSH fermentation from keratin and to a greater extent from soy protein using protease-displaying yeast yielded greater GSH productivity compared to GSH fermentation with amino acid supplementation. This protease-displaying yeast is potentially applicable to a variety of processes for the bio-production of value-added chemicals from proteinaceous biomass resources. PMID:22075633

  12. Biofuel production by liquefaction of kenaf (Hibiscus cannabinus L.) biomass.

    PubMed

    Meryemoğlu, Bahar; Hasanoğlu, Arif; Irmak, Sibel; Erbatur, Oktay

    2014-01-01

    In this study, kenaf biomass, its dried hydrolysate residue (solid residue left after removing water from hydrolysate) and non-hydrolyzed kenaf residue (solid residue left after hydrolysis process) were liquefied at various temperatures. Hydrolysis of biomass was performed in subcritical water condition. The oil+gas yield of biomass materials increased as the temperature increased from 250 to 300°C. Increasing temperature to 350°C resulted in decreases in oil+gas contents for all biomass feeds studied. On the other hand, preasphaltene+asphaltene (PA+A) and char yields significantly decreased with increasing the process temperature. The use of carbon or activated carbon supported Ru catalyst in the process significantly decreased char and PA+A formations. Oils produced from liquefaction of kenaf, dried kenaf hydrolysate and non-hydrolyzed kenaf residue consist of fuel related components such as aromatic hydrocarbons, benzene and benzene derivative compounds, indane and trans/cis-decalin. PMID:24262837

  13. How-To-Do-It: Measuring Vegetation Biomass and Production.

    ERIC Educational Resources Information Center

    Collins, Don; Weaver, T.

    1988-01-01

    Describes a lab exercise used to demonstrate the measurement of biomass in a three layered forest. Discusses sampling, estimation methods, and the analysis of results. Presents an example of a summary sheet for this activity. (CW)

  14. Optimization of Biomass and 5-Aminolevulinic Acid Production by Rhodobacter sphaeroides ATCC17023 via Response Surface Methodology.

    PubMed

    Liu, Shuli; Zhang, Guangming; Li, Jianzheng; Li, Xiangkun; Zhang, Jie

    2016-06-01

    Microbial 5-aminolevulinic acid (ALA) produced from wastewater is considered as potential renewable energy. However, many hurdles are needed to be overcome such as the regulation of key influencing factors on ALA yield. Biomass and ALA production by Rhodobacter sphaeroides was optimized using response surface methodology. The culturing medium was artificial volatile fatty acids wastewater. Three additives were optimized, namely succinate and glycine that are precursors of ALA biosynthesis, and D-glucose that is an inhibitor of ALA dehydratase. The optimal conditions were achieved by analyzing the response surface plots. Statistical analysis showed that succinate at 8.56 mmol/L, glycine at 5.06 mmol/L, and D-glucose at 7.82 mmol/L were the best conditions. Under these optimal conditions, the highest biomass production and ALA yield of 3.55 g/L and 5.49 mg/g-biomass were achieved. Subsequent verification experiments at optimal values had the maximum biomass production of 3.41 ± 0.002 g/L and ALA yield of 5.78 ± 0.08 mg/g-biomass. PMID:26875086

  15. A Site-Related Suitability Analysis for the Production of Biomass as a Contribution to Sustainable Regional Land-Use

    NASA Astrophysics Data System (ADS)

    Förster, Michael; Helms, Yvonne; Herberg, Alfred; Köppen, Antje; Kunzmann, Kathrin; Radtke, Dörte; Ross, Lutz; Itzerott, Sibylle

    2008-04-01

    The use of renewable energy in Europe offers the possibility of reducing greenhouse gas emissions, and contributes to energy security and independence. With the reform of the Common Agricultural Policy (CAP) and a variety of recently introduced national directives supporting renewable energy sources in the European Union, the economic attractiveness of bioenergy production has distinctly increased. This article combines an economic evaluation of biomass production with site-related natural conditions of the Havelland region, situated in the north-east area of Germany. Two methods for evaluating site-specific potential biomass yields were compared. For three example biomass crops, evaluations of yield estimations at agricultural lots for site-optimized suitability (SOS) and conventional suitability (CS) were carried out. Both modelling approaches were compared. The results of the GIS modelling indicate that the financial support for increasing the use of renewable energy with the German feed-in system, called Erneuerbare-Energien-Gesetz (EEG), will possibly lead to an increased cultivation of crops with high biomass output. This monocultural orientation of farming practices and the negative effects on the ecosystem could act in opposition to other environmental initiatives of the EU. The outputs of the SOS analysis show that high biomass production could be integrated into environmental policy proposals. Therefore, new EU policy should take modified subsidies into consideration in order to avoid developing conflicts between small-scale changes in landscape ecosystems caused by large-scale transformations in energy policy.

  16. Production of Biomass-Degrading Multienzyme Complexes under Solid-State Fermentation of Soybean Meal Using a Bioreactor

    PubMed Central

    Vitcosque, Gabriela L.; Fonseca, Rafael F.; Rodríguez-Zúñiga, Ursula Fabiola; Bertucci Neto, Victor; Couri, Sonia; Farinas, Cristiane S.

    2012-01-01

    Biomass-degrading enzymes are one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels. This work evaluates the effects of operational conditions on biomass-degrading multienzyme production by a selected strain of Aspergillus niger. The fungus was cultivated under solid-state fermentation (SSF) of soybean meal, using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The effects of air flow rate, inlet air relative humidity, and initial substrate moisture content on multienzyme (FPase, endoglucanase, and xylanase) production were evaluated using a statistical design methodology. Highest production of FPase (0.55 IU/g), endoglucanase (35.1 IU/g), and xylanase (47.7 IU/g) was achieved using an initial substrate moisture content of 84%, an inlet air humidity of 70%, and a flow rate of 24 mL/min. The enzymatic complex was then used to hydrolyze a lignocellulosic biomass, releasing 4.4 g/L of glucose after 36 hours of saccharification of 50 g/L pretreated sugar cane bagasse. These results demonstrate the potential application of enzymes produced under SSF, thus contributing to generate the necessary technological advances to increase the efficiency of the use of biomass as a renewable energy source. PMID:23365723

  17. Growth kinetics of Chlorococcum humicola - A potential feedstock for biomass with biofuel properties.

    PubMed

    Thomas, Jibu; Jayachithra, E V

    2015-11-01

    Economically viable production facilities for microalgae depend on the optimization of growth parameters with regard to nutrient requirements. Using microalgae to treat industrial effluents containing heavy metals presents an alternative to the current practice of using physical and chemical methods. Present work focuses on the statistical optimization of growth of Chlorococcum humicola to ascertain the maximum production of biomass. Plackett Burman design was carried out to screen the significant variables influencing biomass production. Further, Response Surface Methodology was employed to optimize the effect of inoculum, light intensity and pH on net biomass yield. Optimum conditions for maximum biomass yield were identified to be inoculum at 15%, light intensity to be 1500lx and pH 8.5. Theoretical and predicted values were in agreement and thus the model was found to be significant. Gas chromatography analyses of the FAME derivatives showed a high percentage of saturated fatty acids thereby confirming the biofuel properties of the oil derived from algal biomass. PMID:25791468

  18. Production of new biomass/waste-containing solid fuels

    SciTech Connect

    Akers, D.; Shirey, G.; Zitron, Z.; Nowak, M.

    2000-07-01

    The electric utility industry is interested in the use of biomass and waste byproducts as fuel to reduce both emissions and fuel costs. Unfortunately, biomass and waste byproducts can be troublesome fuels because of low bulk density, high moisture content, variable composition, handling and feeding problems, and inadequate information about combustion and emissions characteristics. One method of addressing these issues is to produce composite fuels composed of a pelletized mixture of biomass and other constituents. However, for composite fuels to be extensively used in the US, especially in the steam market, a lower cost method of producing these fuels must be developed. Also, standard formulations of biomass and coal (possibly including waste) with broad application to US boilers must be identified. In addition to acceptable cost, these standard formulations can provide environmental benefits relative to coal. The Department of Energy along with the Electric Power Research Institute and various industry partners has funded CQ Inc. to develop both a dewatering/pelletizing die and three standard formulations of biomass, coal, and waste byproducts. Six biomass/waste sources were initially selected for study: petroleum coke, mixed waste plastic, switchgrass, waxed cardboard, poultry manure, and sewage sludge. A sample representative of each source was collected and analyzed. Also, two sources of coal, recovered from waste ponds, were collected for use in the project.

  19. Optimization of Biofuel and Biochar Production from the Slow Pyrolysis of Biomass

    NASA Astrophysics Data System (ADS)

    Fang, J.; Gao, B.; Nsf Reu in Water Resources

    2010-12-01

    Slow pyrolysis was performed on biomass samples (i.e., energy cane and air potato) to determine the most energy efficient conditions for producing biofuel and biochar. The potential of air potato as a source of fuel and char was also investigated. Dry biomass samples of 10, 15 and 20 g were heated in a reactor at a final temperatures of 300, 450, or 600 °C, and the minimum amount of time required to complete pyrolysis was recorded. Maximum biochar yield was obtained at 300°C for both energy cane and air potato at all masses, and maximum bio-oil yield was obtained at 450°C for all samples. Pyrolysis required the least amount of time at 450°C. Bio-oil yields for air potato were slightly lower than that of energy cane, while biochar yield was slightly higher. Since air potato showed similar product yields to energy cane, this indicates it has potential to be a good feedstock for biofuel and biochar productions.

  20. Increased mycelial biomass production by Lentinula edodes intermittently illuminated by green light emitting diodes.

    PubMed

    Glukhova, Lubov B; Sokolyanskaya, Ludmila O; Plotnikov, Evgeny V; Gerasimchuk, Anna L; Karnachuk, Olga V; Solioz, Marc; Karnachuk, Raisa A

    2014-11-01

    Fungi possess a range of light receptors to regulate metabolism and differentiation. To study the effect of light on Lentinula edodes (the shiitake mushroom), mycelial cultures were exposed to blue, green, and red fluorescent lights and light-emitting diodes, as well as green laser light. Biomass production, morphology, and pigment production were evaluated. Exposure to green light at intervals of 1 min/d at 0.4 W/m(2) stimulated biomass production by 50-100 %, depending on the light source. Light intensities in excess of 1.8 W/m(2) or illumination longer than 30 min/d did not affect biomass production. Carotenoid production and morphology remained unaltered during increased biomass production. These observations provide a cornerstone to the study of photoreception by this important fungus. PMID:25048231

  1. The Giant Knotweed (Fallopia sachalinensis var. Igniscum) as a new plant resource for biomass production for bioenergy

    NASA Astrophysics Data System (ADS)

    Lebzien, S.; Veste, M.; Fechner, H.; Koning, L.; Mantovani, D.; Freese, D.

    2012-04-01

    The cultivation of bioenergy crop for energetic biomass production and biogas will increase in the next decades in Europe and the world. In Germany maize is the most commonly used energy crops for biogas. To optimize the sustainability of bioenergy crop production new land management systems and crop species are needed. Herbaceous perennials have a great potential to fulfill this requirement. A new species for bioenergy production is the Giant Knotweed or Sakhalin Knotweed (Fallopia sachalinensis F. Schmidt ex Maxim., Fam. Polygonaceae) The knotweed is originated from Sakhalin, Korea and Japan .The plant is characterized by a high annual biomass production and can reach heights up to 3-4 m. As a new bioenergy crop the new cultivars IGNISCUM Basic (R) and IGNISCUM Candy (R) were cultured from the wild form and commercially used. Important is that both cultivars are not invasive. IGNISCUM Basic is used for combined heat and power plants. IGNISCUM Candy can be harvested 2-3 times during the growing season and the green biomass can be used for biogas production. Comprehensive test series are carried out to analyze the biogas. First results from lab investigations and experiments in biogas plants show that fresh matter of IGNISCUM Candy can well substitute maize as substrate in biogas power plants. Yields per hectare and the amount of biogas per ton of organic dry matter can be considered as almost equal to maize. Concerning the wooden biomass of IGNISCUM Basic values of combustion can be compared with wood chips from forest trees. For a sustainable and optimal production of biomass we develop cultivation technology for this species. Field experiments are arranged under different climatic and soil conditions across Germany from Schleswig-Holstein to southern Germany to investigate the plant growth and biomass production on the field scale. Physiological parameters are determined for the relations between growth stages, chlorophyll content, photosynthesis and plant

  2. Potential of Co-firing of Woody Biomass in Coal Fired Power Plant

    NASA Astrophysics Data System (ADS)

    Makino, Yosuke; Kato, Takeyoshi; Suzuoki, Yasuo

    Taking the distributing woody biomass supply into account, this paper assesses the potential of a co-firing of woody biomass in utility's coal power plant from the both energy-saving and economical view points. Sawmill wastes, trimming wastes from fruit farms and streets, and thinning residues from forests in Aichi Prefecture are taken into account. Even though transportation energy is required, almost all of woody biomass can be more efficiently used in co-firing with coal than in a small-scale fuel cell system with gasification as a distributed utilization. When the capital cost of fuel cell system with 25% of total efficiency, including preprocess, gasification and power generation, is higher than 170× 103yen/kW, almost all of thinning residues can be more economically used in co-firing. The cost of woody biomass used in co-firing is also compared with the transaction cost of renewable power in the current RPS scheme. The result suggests the co-firing of woody biomass in coal fired power plant can be feasible measure for effective utilization of woody biomass.

  3. Design Case Summary: Production of Gasoline and Diesel from Biomass via Fast Pyrolysis, Hydrotreating, and Hydrocracking

    SciTech Connect

    Jones, S. B.; Valkenburg, C.; Walkton, C. W.; Elliott, D. C.; Holladay, J. E.; Stevens, D. J.; Kinchin, C.; Czernik, S.

    2010-02-01

    The Biomass Program develops design cases to understand the current state of conversion technologies and to determine where improvements need to take place in the future. This design case is the first to establish detailed cost targest for the production of diesel and gasoline blendstock from biomass via a fast pyrolysis process.

  4. Issues in the production and conversion of lignocellulosic biomass crops to ethanol

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The goal of replacing 30% of 2004 gasoline demand with biofuels by 2030 will require 1 billion tons of biomass annually. Ethanol from lignocellulosic biomass (crop residues and perennial energy crops) will contribute the lion's share of biofuel production. Among challenges to overcome is environment...

  5. Species richness and the temporal stability of biomass production: A new analysis of recent biodiversity experiments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this study, we investigate how species richness affects temporal stability of biomass production by analyzing 27 recent biodiversity experiments conducted in grassland and freshwater algal communities. We find that, in grasslands, increasing species richness stabilizes whole-community biomass pro...

  6. Lignin-Derived Carbon Fiber as a Co-Product of Refining Cellulosic Biomass

    SciTech Connect

    Langholtz, Matthew H.; Downing, Mark; Graham, Robin Lambert; Baker, Fred S.; Compere, Alicia L.; William L. Griffith; Boeman, Raymond G.; Keller, Martin

    2014-01-15

    Lignin by-products from biorefineries has the potential to provide a low-cost alternative to petroleum-based precursors to manufacture carbon fiber, which can be combined with a binding matrix to produce a structural material with much greater specific strength and specific stiffness than conventional materials such as steel and aluminum. The market for carbon fiber is universally projected to grow exponentially to fill the needs of clean energy technologies such as wind turbines and to improve the fuel economies in vehicles through lightweighting. In addition to cellulosic biofuel production, lignin-based carbon fiber production coupled with biorefineries may provide $2,400 to $3,600 added value dry Mg-1 of biomass for vehicle applications. Compared to producing ethanol alone, the addition of lignin-derived carbon fiber could increase biorefinery gross revenue by 30% to 300%. Using lignin-derived carbon fiber in 15 million vehicles per year in the US could reduce fossil fuel consumption by 2-5 billion liters year-1, reduce CO2 emissions by about 6.7 million Mg year-1, and realize fuel savings through vehicle lightweighting of $700 to $1,600 per Mg biomass processed. The value of fuel savings from vehicle lightweighting becomes economical at carbon fiber price of $6.60 kg-1 under current fuel prices, or $13.20 kg-1 under fuel prices of about $1.16 l-1.

  7. Cover crop biomass production and water use in the central great plains under varying water availability

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The water-limited environment of the semi-arid central Great Plains may not have potential to produce enough cover crop biomass to generate benefits associated with cover crop use in more humid regions. There have been reports that cover crops grown in mixtures produce more biomass with greater wate...

  8. Simplifying biodiesel production: the direct or 'in situ' transesterification of algal biomass

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ‘in situ’ esterification/transesterification of algal biomass lipids to produce fatty acid methyl esters (FAME), for potential use as biodiesel, was investigated. Commercial algal biomass was employed, containing 20.9 wt percent hexane extractable oil. This consisted of 35.1 wt percent free fa...

  9. Maximizing Production of Fiber, Fermentable Sugars, and Energy by Matching Biomass Species to Landscape Position

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of this research project is to characterize a diverse group of potential biomass species (corn, alfalfa, perennial flax, switchgrass, poplar, and willow) for their yield of biomass, fiber, and fermentable sugars across a diverse set of within-field environments. Our goal is to advance ...

  10. Benthic oxygen uptake, hydrolytic potentials and microbial biomass at the Arctic continental slope

    NASA Astrophysics Data System (ADS)

    Boetius, Antje; Damm, Ellen

    1998-02-01

    Oxygen (O 2) uptake and microbial activity in sediments of the eastern Arctic continental slope were investigated in both ice-covered and ice-free areas of the Laptev Sea. Total O 2 flux ( J) decreased markedly from 2 mmol m -2 d -1 at the shelf edge (50 m) to 0.07 mmol m -2 d -1 at the bottom of the slope (3500 m), matched by the more than tenfold decline in chlorophyll pigments (CPE), protein and dissolved amino acids (DFAA). Furthermore, concentrations of these labile organic compounds were strongly correlated with extracellular enzyme potentials (EEA) in the sediments as well as with microbial biomass. The concentrations of labile substances and total microbial biomass (TMB) as well as the rates of O 2 uptake and EEA were independent of the distribution of TOC, probably due to the dominance of non-labile terrigenous compounds. Differences in O 2 uptake and microbial EEA between ice-covered and ice-free transects were relatively small. Values of O 2 uptake, CPE, EEA and TMB at the Laptev Sea slope were considerably lower than at temperate continental slopes but nevertheless higher than in the central Arctic deep-sea basin. Considering newly published data on primary productivity in the central Arctic, our results indicate that the benthic respiratory demand at the Laptev Sea slope and in the Arctic basin could be satisfied by the vertical flux of POC and does not necessarily depend on lateral advection of POC from the shelf seas as previously anticipated.

  11. Research to develop improved production methods for woody and herbaceous biomass crops

    SciTech Connect

    Ferrell, J.E.; Wright, L.L.; Tuskan, G.A.

    1995-09-01

    DOE`s Biofuels Feedstock Development Program (BFDP) has led the nation in developing short-rotation woody crops (SRWC) and herbaceous energy crops (HEC) as feedstocks for renewable energy. Over the past 15 years, the BFDP has examined the performance of 154 woody species and 35 herbaceous species in field trials across the US. One result of this effort to date has been the prescription of silvicultural systems for hybrid poplars and hybrid willows and agricultural systems for switchgrass. Selected clones of woody species are producing dry weight yields in research plots on agricultural land that are 3 to 7 times greater than those obtained from mixed species stands on forest land, and at least 2 times the yields of southern plantation pines. Selected switchgrass varieties are producing dry weight yields 2 to 7 times greater than average forage grass yields on similar sites. Crop development research is continuing efforts to translate this potential, in a sustainable manner, to larger, more geographically diverse acreage. Research on environmental aspects of biomass crop production are aimed at developing sustainable systems that will contribute to the biodiversity of agricultural landscapes. Systems integration aims to understand all factors affecting bringing the crop to market. Factors affecting price and potential supplies of biomass crops are being evaluated at regional and national scales. Scale-up studies, feasibility analysis and demonstrations are establishing actual costs and facilitating the commercialization of integrated biomass systems. Information management and dissemination activities are facilitating the communication of results among a community of researchers, policymakers, and potential users and producers of energy crops.

  12. Modeling Reef Fish Biomass, Recovery Potential, and Management Priorities in the Western Indian Ocean.

    PubMed

    McClanahan, Timothy R; Maina, Joseph M; Graham, Nicholas A J; Jones, Kendall R

    2016-01-01

    Fish biomass is a primary driver of coral reef ecosystem services and has high sensitivity to human disturbances, particularly fishing. Estimates of fish biomass, their spatial distribution, and recovery potential are important for evaluating reef status and crucial for setting management targets. Here we modeled fish biomass estimates across all reefs of the western Indian Ocean using key variables that predicted the empirical data collected from 337 sites. These variables were used to create biomass and recovery time maps to prioritize spatially explicit conservation actions. The resultant fish biomass map showed high variability ranging from ~15 to 2900 kg/ha, primarily driven by human populations, distance to markets, and fisheries management restrictions. Lastly, we assembled data based on the age of fisheries closures and showed that biomass takes ~ 25 years to recover to typical equilibrium values of ~1200 kg/ha. The recovery times to biomass levels for sustainable fishing yields, maximum diversity, and ecosystem stability or conservation targets once fishing is suspended was modeled to estimate temporal costs of restrictions. The mean time to recovery for the whole region to the conservation target was 8.1(± 3SD) years, while recovery to sustainable fishing thresholds was between 0.5 and 4 years, but with high spatial variation. Recovery prioritization scenario models included one where local governance prioritized recovery of degraded reefs and two that prioritized minimizing recovery time, where countries either operated independently or collaborated. The regional collaboration scenario selected remote areas for conservation with uneven national responsibilities and spatial coverage, which could undermine collaboration. There is the potential to achieve sustainable fisheries within a decade by promoting these pathways according to their social-ecological suitability. PMID:27149673

  13. Modeling Reef Fish Biomass, Recovery Potential, and Management Priorities in the Western Indian Ocean

    PubMed Central

    McClanahan, Timothy R.; Maina, Joseph M.; Graham, Nicholas A. J.; Jones, Kendall R.

    2016-01-01

    Fish biomass is a primary driver of coral reef ecosystem services and has high sensitivity to human disturbances, particularly fishing. Estimates of fish biomass, their spatial distribution, and recovery potential are important for evaluating reef status and crucial for setting management targets. Here we modeled fish biomass estimates across all reefs of the western Indian Ocean using key variables that predicted the empirical data collected from 337 sites. These variables were used to create biomass and recovery time maps to prioritize spatially explicit conservation actions. The resultant fish biomass map showed high variability ranging from ~15 to 2900 kg/ha, primarily driven by human populations, distance to markets, and fisheries management restrictions. Lastly, we assembled data based on the age of fisheries closures and showed that biomass takes ~ 25 years to recover to typical equilibrium values of ~1200 kg/ha. The recovery times to biomass levels for sustainable fishing yields, maximum diversity, and ecosystem stability or conservation targets once fishing is suspended was modeled to estimate temporal costs of restrictions. The mean time to recovery for the whole region to the conservation target was 8.1(± 3SD) years, while recovery to sustainable fishing thresholds was between 0.5 and 4 years, but with high spatial variation. Recovery prioritization scenario models included one where local governance prioritized recovery of degraded reefs and two that prioritized minimizing recovery time, where countries either operated independently or collaborated. The regional collaboration scenario selected remote areas for conservation with uneven national responsibilities and spatial coverage, which could undermine collaboration. There is the potential to achieve sustainable fisheries within a decade by promoting these pathways according to their social-ecological suitability. PMID:27149673

  14. Combined heat treatment and acid hydrolysis of cassava grate waste (CGW) biomass for ethanol production

    SciTech Connect

    Agu, R.C.; Amadife, A.E.; Ude, C.M.; Onyia, A.; Ogu, E.O.; Okafor, M.; Ezejiofor, E.

    1997-12-31

    The effect of combined heat treatment and acid hydrolysis (various concentrations) on cassava grate waste (CGW) biomass for ethanol production was investigated. At high concentrations of H{sub 2}SO{sub 4} (1--5 M), hydrolysis of the CGW biomass was achieved but with excessive charring or dehydration reaction. At lower acid concentrations, hydrolysis of CGW biomass was also achieved with 0.3--0.5 M H{sub 2}SO{sub 4}, while partial hydrolysis was obtained below 0.3 M H{sub 2}SO{sub 4} (the lowest acid concentration that hydrolyzed CGW biomass) at 120 C and 1 atm pressure for 30 min. A 60% process efficiency was achieved with 0.3 M H{sub 2}SO{sub 4} in hydrolyzing the cellulose and lignin materials present in the CGW biomass. High acid concentration is therefore not required for CGW biomass hydrolysis. The low acid concentration required for CGW biomass hydrolysis, as well as the minimal cost required for detoxification of CGW biomass because of low hydrogen cyanide content of CGW biomass would seem to make this process very economical. From three liters of the CGW biomass hydrolysate obtained from hydrolysis with 0.3M H{sub 2}SO{sub 4}, ethanol yield was 3.5 (v/v%) after yeast fermentation. However, although the process resulted in gainful utilization of CGW biomass, additional costs would be required to effectively dispose new by-products generated from CGW biomass processing.

  15. Geographic analysis of the feasibility of collocating algal biomass production with wastewater treatment plants.

    PubMed

    Fortier, Marie-Odile P; Sturm, Belinda S M

    2012-10-16

    Resource demand analyses indicate that algal biodiesel production would require unsustainable amounts of freshwater and fertilizer supplies. Alternatively, municipal wastewater effluent can be used, but this restricts production of algae to areas near wastewater treatment plants (WWTPs), and to date, there has been no geospatial analysis of the feasibility of collocating large algal ponds with WWTPs. The goals of this analysis were to determine the available areas by land cover type within radial extents (REs) up to 1.5 miles from WWTPs; to determine the limiting factor for algal production using wastewater; and to investigate the potential algal biomass production at urban, near-urban, and rural WWTPs in Kansas. Over 50% and 87% of the land around urban and rural WWTPs, respectively, was found to be potentially available for algal production. The analysis highlights a trade-off between urban WWTPs, which are generally land-limited but have excess wastewater effluent, and rural WWTPs, which are generally water-limited but have 96% of the total available land. Overall, commercial-scale algae production collocated with WWTPs is feasible; 29% of the Kansas liquid fuel demand could be met with implementation of ponds within 1 mile of all WWTPs and supplementation of water and nutrients when these are limited. PMID:22970803

  16. Production of Renewable Natural Gas from Waste Biomass

    NASA Astrophysics Data System (ADS)

    Kumar, Sachin; Suresh, S.; Arisutha, S.

    2013-03-01

    Biomass energy is expected to make a major contribution to the replacement of fossil fuels. Methane produced from biomass is referred to as bio-methane, green gas, bio-substitute natural gas or renewable natural gas (RNG) when it is used as a transport fuel. Research on upgrading of the cleaned producer gas to RNG is still ongoing. The present study deals with the conversion of woody biomass into fuels, RNG using gasifier. The various effects of parameters like temperature, pressure, and tar formation on conversion were also studied. The complete carbon conversion was observed at 480 °C and tar yield was significantly less. When biomass was gasified with and without catalyst at about 28 s residence time, ~75 % (w/w) and 88 % (w/w) carbon conversion for without and with catalyst was observed. The interest in RNG is growing; several initiatives to demonstrate the thermal-chemical conversion of biomass into methane and/or RNG are under development.

  17. Biomass yielding potential of naturally regenerated Prosopis juliflora tree stands at three varied ecosystems in southern districts of Tamil Nadu, India.

    PubMed

    Saraswathi, K; Chandrasekaran, S

    2016-05-01

    Fuel energy demand is of great concern in recent times due to the depletion of fossil fuel resources. Biomass serves as widely available primary renewable energy source. Hence, a study was performed to assess the above-ground biomass yielding capability of fuel wood tree Prosopis juliflora in three varied ecosystems viz., coastal, fallow land and riparian ecosystems in southern districts of Tamil Nadu. The results showed that the biomass production potential and above-ground net primary productivity of P. juliflora depend on the age of the tree stands and the nature of ecosystem. A higher biomass yield was observed for P. juliflora trees with 5 to 10 years old when compared to less than 5 years of their age. Among the three ecosystems, the maximum biomass production was recorded in riparian ecosystem. The stands with less than 5-year-old P. juliflora trees gave 1.40 t/ha, and 5- to 10-year-old tree stands produced 27.69 t/ha in riparian ecosystem. Above-ground net primary productivity of both the age groups was high in fallow land ecosystem. In riparian ecosystem, the wood showed high density and low sulphur content than the other two ecosystems. Hence, P. juliflora biomass can serve as an environmentally and economically feasible fuel as well as their utilization proffers an effective means to control its invasiveness. PMID:26797948

  18. Plankton secondary productivity and biomass: Their relation to lake trophic state

    USGS Publications Warehouse

    Pederson, G.L.; Welch, E.B.; Litt, A.H.

    1976-01-01

    The biomass and production of the most important zooplankton species were followed for two years in three lakes of varying trophic status in the Lake Washington watershed. Cladocerans and copepods were of equal importance in the biomass of lakes Findley and Chester Morse (both oligotrophic), whereas, copepods were the main biomass component in Lake Sammamish (mesotrophic). Cladocerans dominated production in lakes Sammamish and Chester Morse, while in Findley Lake their productive role, like that of biomass, was equal to that of the copepods. Rotifers contributed a relatively small biomass and production. Data from this study supported Hillbricht-Ilkowska's postulate that the energy transfer efficiency between the primary and secondary trophic levels decreases with increasing trophic state. Energy transfer efficiencies for the lakes of this study expressed as a two year mean of the ratio-secondary: primary production, were as follows: Findley Lake-0.13; Chester Morse Lake-0.08; and Lake Sammanish-0.04. On the other hand, the hypothesis of Patalas that the secondary productivity: biomass ratio (P/B) tended to increase in proportion to the productivity of a lake, could not be supported. Lake Sammamish, the most productive of the lakes studied, had a P/B ratio of 0.03 while lakes Findley and Chester Morse had P/B ratios of 0.04. ?? 1976 Dr. W. Junk b. v. Publishers.

  19. Pretreatment of Biomass by Aqueous Ammonia for Bioethanol Production

    NASA Astrophysics Data System (ADS)

    Kim, Tae Hyun; Gupta, Rajesh; Lee, Y. Y.

    The methods of pretreatment of lignocellulosic biomass using aqueous ammonia are described. The main effect of ammonia treatment of biomass is delignification without significantly affecting the carbohydrate contents. It is a very effective pretreatment method especially for substrates that have low lignin contents such as agricultural residues and herbaceous feedstock. The ammonia-based pretreatment is well suited for simultaneous saccharification and co-fermentation (SSCF) because the treated biomass retains cellulose as well as hemicellulose. It has been demonstrated that overall ethanol yield above 75% of the theoretical maximum on the basis of total carbohydrate is achievable from corn stover pretreated with aqueous ammonia by way of SSCF. There are two different types of pretreatment methods based on aqueous ammonia: (1) high severity, low contact time process (ammonia recycle percolation; ARP), (2) low severity, high treatment time process (soaking in aqueous ammonia; SAA). Both of these methods are described and discussed for their features and effectiveness.

  20. Silica distinctively affects cell wall features and lignocellulosic saccharification with large enhancement on biomass production in rice.

    PubMed

    Zhang, Jing; Zou, Weihua; Li, Ying; Feng, Yongqing; Zhang, Hui; Wu, Zhiliang; Tu, Yuanyuan; Wang, Yanting; Cai, Xiwen; Peng, Liangcai

    2015-10-01

    Rice is a typical silicon-accumulating crop with enormous biomass residues for biofuels. Silica is a cell wall component, but its effect on the plant cell wall and biomass production remains largely unknown. In this study, a systems biology approach was performed using 42 distinct rice cell wall mutants. We found that silica levels are significantly positively correlated with three major wall polymers, indicating that silica is associated with the cell wall network. Silicon-supplied hydroculture analysis demonstrated that silica distinctively affects cell wall composition and major wall polymer features, including cellulose crystallinity (CrI), arabinose substitution degree (reverse Xyl/Ara) of xylans, and sinapyl alcohol (S) proportion in three typical rice mutants. Notably, the silicon supplement exhibited dual effects on biomass enzymatic digestibility in the mutant and wild type (NPB) after pre-treatments with 1% NaOH and 1% H2SO4. In addition, silicon supply largely enhanced plant height, mechanical strength and straw biomass production, suggesting that silica rescues mutant growth defects. Hence, this study provides potential approaches for silicon applications in biomass process and bioenergy rice breeding. PMID:26398793

  1. Potential biomass reductions to Miscanthus × giganteus by stem-boring caterpillars

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Injury from stem-boring caterpillars has been observed on the perennial grass Miscanthus × giganteus Greef and Deuter ex Hodkinson and Renvoize in both its native and introduced ranges. Because some species causing stem injury in the United States have not been identified, potential biomass reducti...

  2. Microwave-Assisted γ-Valerolactone Production for Biomass Lignin Extraction: A Cascade Protocol.

    PubMed

    Tabasso, Silvia; Grillo, Giorgio; Carnaroglio, Diego; Calcio Gaudino, Emanuela; Cravotto, Giancarlo

    2016-01-01

    The general need to slow the depletion of fossil resources and reduce carbon footprints has led to tremendous effort being invested in creating "greener" industrial processes and developing alternative means to produce fuels and synthesize platform chemicals. This work aims to design a microwave-assisted cascade process for a full biomass valorisation cycle. GVL (γ-valerolactone), a renewable green solvent, has been used in aqueous acidic solution to achieve complete biomass lignin extraction. After lignin precipitation, the levulinic acid (LA)-rich organic fraction was hydrogenated, which regenerated the starting solvent for further biomass delignification. This process does not requires a purification step because GVL plays the dual role of solvent and product, while the reagent (LA) is a product of biomass delignification. In summary, this bio-refinery approach to lignin extraction is a cascade protocol in which the solvent loss is integrated into the conversion cycle, leading to simplified methods for biomass valorisation. PMID:27023511

  3. Lipid extraction methods from microalgal biomass harvested by two different paths: screening studies toward biodiesel production.

    PubMed

    Ríos, Sergio D; Castañeda, Joandiet; Torras, Carles; Farriol, Xavier; Salvadó, Joan

    2013-04-01

    Microalgae can grow rapidly and capture CO2 from the atmosphere to convert it into complex organic molecules such as lipids (biodiesel feedstock). High scale economically feasible microalgae based oil depends on optimizing the entire process production. This process can be divided in three very different but directly related steps (production, concentration, lipid extraction and transesterification). The aim of this study is to identify the best method of lipid extraction to undergo the potentiality of some microalgal biomass obtained from two different harvesting paths. The first path used all physicals concentration steps, and the second path was a combination of chemical and physical concentration steps. Three microalgae species were tested: Phaeodactylum tricornutum, Nannochloropsis gaditana, and Chaetoceros calcitrans One step lipid extraction-transesterification reached the same fatty acid methyl ester yield as the Bligh and Dyer and soxhlet extraction with n-hexane methods with the corresponding time, cost and solvent saving. PMID:23434816

  4. High Potential Source for Biomass Degradation Enzyme Discovery and Environmental Aspects Revealed through Metagenomics of Indian Buffalo Rumen

    PubMed Central

    Singh, K. M.; Reddy, Bhaskar; Patel, Dishita; Patel, A. K.; Patel, J. B.; Joshi, C. G.

    2014-01-01

    The complex microbiomes of the rumen functions as an effective system for plant cell wall degradation, and biomass utilization provide genetic resource for degrading microbial enzymes that could be used in the production of biofuel. Therefore the buffalo rumen microbiota was surveyed using shot gun sequencing. This metagenomic sequencing generated 3.9 GB of sequences and data were assembled into 137270 contiguous sequences (contigs). We identified potential 2614 contigs encoding biomass degrading enzymes including glycoside hydrolases (GH: 1943 contigs), carbohydrate binding module (CBM: 23 contigs), glycosyl transferase (GT: 373 contigs), carbohydrate esterases (CE: 259 contigs), and polysaccharide lyases (PE: 16 contigs). The hierarchical clustering of buffalo metagenomes demonstrated the similarities and dissimilarity in microbial community structures and functional capacity. This demonstrates that buffalo rumen microbiome was considerably enriched in functional genes involved in polysaccharide degradation with great prospects to obtain new molecules that may be applied in the biofuel industry. PMID:25136572

  5. Biomass burning and the production of greenhouse gases

    NASA Technical Reports Server (NTRS)

    Levine, Joel S.

    1991-01-01

    The present discussion of related aspects of biomass burning describes a technique for estimating the instantaneous emission of trace gases generated by such fires on the basis of satellite imagery, and notes that burning results in significantly enhanced biogenic emissions of N2O, NO, and CH4. Biomass burning therefore has both immediate and long-term impacts on the trace-gas content of the atmosphere. The effects of Kuwait's oil fires, which encompass both combustion gases and particulates, are compared with those of the more general problem.

  6. The utilization of post-chlorinated municipal domestic wastewater for biomass and lipid production by Chlorella spp. under batch conditions.

    PubMed

    Mutanda, Taurai; Karthikeyan, Subburammu; Bux, Faizal

    2011-08-01

    South Africa has a rich microalgal biodiversity which has the potential to be used for renewable bio-fuel production in the region. Bioprospecting for oleaginous microalgae in KwaZulu Natal Province, South Africa, resulted in the establishment of a microalgal culture collection system for alternative energy research in the country. A potential hyper-lipid-producing Chlorella spp. strain was isolated, purified, and cultured in supplemented post-chlorinated wastewater for biomass and lipid production at the laboratory scale under batch mode. The microalgal strain was cultivated in different strengths of BG-11 media supplemented with wastewater from a local municipal domestic wastewater treatment plant. The Chlorella spp. was grown using ambient dissolved carbon dioxide in shake flasks under photosynthetically active radiation (±120 μmol m⁻² s⁻¹). Microalgal biomass and lipid productivity were monitored at 24-h intervals in the batch mode. The microalgal biomass was analyzed by direct light microscopy and indirectly by spectrophotometry at 600 nm, and the lipids were extracted and quantified. The growth rate of the Chlorella spp. was enhanced in post-chlorinated wastewater supplemented with 5 mM NaNO₃ with maximal biomass productivity. A dramatic increase in lipid yield was achieved with the post-chlorinated wastewater supplemented with 25 mM NaNO₃. Low dosages of free chlorine were found to enhance microalgal growth. These findings serve as a basis for further scale-up trials using municipal wastewater as a medium for microalgal biomass and lipid production. PMID:21347654

  7. Biomass energy crop production versus food crop production in the Caribbean

    SciTech Connect

    Sammuels, G.

    1983-12-01

    The Caribbean countries have traditionally grown sugar cane, coffee and bananas as major agriculture export crops. Food crop production was sufficient in most cases for domestic consumption. In recent years powerful social and economic changes of increasing population, industrial development and higher living standards have placed pressure on local governments to provide food, clothing, shelter and energy. Energy that is mainly supplied by imported oil. Biomass, primarily as sugar cane, can provide a solution, either partial or total, to the problem. Unfortunately, the arable land area for the majority of the countries is limited. Food crop production is needed for local consumption and export. Possible energy crop production to provide local needs will place an increasing demand on arable land. The objective of this paper is to present the scope of food versus energy crop production and a suggested renewable energy crop program to help achieve a balance within the limited land resources of the Caribbean.

  8. Research Summary: Corrosion Considerations for Thermochemical Biomass Liquefaction Process Systems in Biofuel Production

    DOE PAGESBeta

    Brady, Michael P; Keiser, James R; Leonard, Donovan N; Whitmer, Lysle; Thomson, Jeffery K

    2014-01-01

    Thermochemical liquifaction processing of biomass to produce bio-derived fuels (e.g. gasoline, jet fuel, diesel, home heating oil, etc.) is of great recent interest as a renewable energy source. Approaches under investigation include direct liquefaction, hydrothermal liquefaction, hydropyrolysis, fast pyrolysis, etc. to produce energy dense liquids that can be utilized as produced or further processed to provide products of higher value. An issue with bio-oils is that they tend to contain significant concentrations of organic compounds, which make the bio-oil acidic and a potential source of corrosion issues in in transport, storage, and use. Efforts devoted to modified/further processing of bio-oilsmore » to make them less corrosive are currently being widely pursued. Another aspect that must also be addressed is potential corrosion issues in the bio-oil liquefaction process equipment itself. Depending on the specific process, bio-oil liquefaction production temperatures can reach up to 400-600 C, and involve the presence of aggressive sulfur, and halide species from both the biomass used and/or process additives. Detailed knowledge of the corrosion resistance of candidate process equipment alloys in these bio-oil production environments is currently lacking. This paper summarizes our recent, ongoing efforts to assess the extent to which corrosion of bio-oil process equipment may be an issue, with the ultimate goal of providing the basis to select the lowest cost alloy grades capable of providing the long-term corrosion resistance needed for future bio-oil production plants.« less

  9. Corrosion Considerations for Thermochemical Biomass Liquefaction Process Systems in Biofuel Production

    NASA Astrophysics Data System (ADS)

    Brady, M. P.; Keiser, J. R.; Leonard, D. N.; Whitmer, L.; Thomson, J. K.

    2014-12-01

    Thermochemical liquefaction processing of biomass to produce bio-derived fuels (e.g., gasoline, jet fuel, diesel, home heating oil, etc.) is of great recent interest as a renewable energy source. Approaches under investigation include direct liquefaction, hydrothermal liquefaction, hydropyrolysis, fast pyrolysis, etc., to produce energy dense liquids that can be utilized as produced or further processed to provide products of higher value. An issue with bio-oils is that they tend to contain significant concentrations of organic oxygenates, including acids, which make the bio-oil a potential source of corrosion issues in transport, storage, and use. Efforts devoted to modified/further processing of bio-oils to make them less corrosive are currently being widely pursued. Another issue that must also be addressed in bio-oil liquefaction is potential corrosion issues in the process equipment. Depending on the specific process, bio-oil liquefaction production temperatures are typically in the 300-600°C range, and the process environment can contain aggressive sulfur and halide species from both the biomass used and/or process additives. Detailed knowledge of the corrosion resistance of candidate process equipment alloys in these bio-oil production environments is currently lacking. This paper summarizes recent, ongoing efforts to assess the extent of corrosion of bio-oil process equipment, with the ultimate goal of providing a basis for the selection of the lowest cost alloy grades capable of providing the long-term corrosion resistance needed for future bio-oil production plants.

  10. Corrosion considerations for thermochemical biomass liquefaction process systems in biofuel production

    SciTech Connect

    Brady, Michael P.; Keiser, James R.; Leonard, Donovan N.; Whitmer, Lysle; Thomson, Jeffery K.

    2014-11-11

    Thermochemical liquifaction processing of biomass to produce bio-derived fuels (e.g. gasoline, jet fuel, diesel, home heating oil, etc.) is of great recent interest as a renewable energy source. Approaches under investigation include direct liquefaction, hydrothermal liquefaction, hydropyrolysis, fast pyrolysis, etc. to produce energy dense liquids that can be utilized as produced or further processed to provide products of higher value. An issue with bio-oils is that they tend to contain significant concentrations of organic compounds, which make the bio-oil acidic and a potential source of corrosion issues in in transport, storage, and use. Efforts devoted to modified/further processing of bio-oils to make them less corrosive are currently being widely pursued. Another aspect that must also be addressed is potential corrosion issues in the bio-oil liquefaction process equipment itself. Depending on the specific process, bio-oil liquefaction production temperatures can reach up to 400-600 °C, and involve the presence of aggressive sulfur, and halide species from both the biomass used and/or process additives. Detailed knowledge of the corrosion resistance of candidate process equipment alloys in these bio-oil production environments is currently lacking. Lastly, this paper summarizes our recent, ongoing efforts to assess the extent to which corrosion of bio-oil process equipment may be an issue, with the ultimate goal of providing the basis to select the lowest cost alloy grades capable of providing the long-term corrosion resistance needed for future bio-oil production plants.

  11. Corrosion considerations for thermochemical biomass liquefaction process systems in biofuel production

    DOE PAGESBeta

    Brady, Michael P.; Keiser, James R.; Leonard, Donovan N.; Whitmer, Lysle; Thomson, Jeffery K.

    2014-11-11

    Thermochemical liquifaction processing of biomass to produce bio-derived fuels (e.g. gasoline, jet fuel, diesel, home heating oil, etc.) is of great recent interest as a renewable energy source. Approaches under investigation include direct liquefaction, hydrothermal liquefaction, hydropyrolysis, fast pyrolysis, etc. to produce energy dense liquids that can be utilized as produced or further processed to provide products of higher value. An issue with bio-oils is that they tend to contain significant concentrations of organic compounds, which make the bio-oil acidic and a potential source of corrosion issues in in transport, storage, and use. Efforts devoted to modified/further processing of bio-oilsmore » to make them less corrosive are currently being widely pursued. Another aspect that must also be addressed is potential corrosion issues in the bio-oil liquefaction process equipment itself. Depending on the specific process, bio-oil liquefaction production temperatures can reach up to 400-600 °C, and involve the presence of aggressive sulfur, and halide species from both the biomass used and/or process additives. Detailed knowledge of the corrosion resistance of candidate process equipment alloys in these bio-oil production environments is currently lacking. Lastly, this paper summarizes our recent, ongoing efforts to assess the extent to which corrosion of bio-oil process equipment may be an issue, with the ultimate goal of providing the basis to select the lowest cost alloy grades capable of providing the long-term corrosion resistance needed for future bio-oil production plants.« less

  12. Use of several waste substrates for carotenoid-rich yeast biomass production.

    PubMed

    Marova, I; Carnecka, M; Halienova, A; Certik, M; Dvorakova, T; Haronikova, A

    2012-03-01

    Carotenoids are industrially significant pigments produced in many bacteria, fungi, and plants. Carotenoid biosynthesis in yeasts is involved in stress response mechanisms. Thus, controlled physiological and nutrition stress can be used for enhanced pigment production. Huge commercial demand for natural carotenoids has focused attention on developing of suitable biotechnological techniques including use of liquid waste substrates as carbon and/or nitrogen source. In this work several red yeast strains (Sporobolomyces roseus, Rhodotorula glutinis, Rhodotorula mucilaginosa) were enrolled into a comparative screening study. To increase the yield of these pigments at improved biomass production, several types of exogenous as well as nutrition stress were tested. Each strain was cultivated at optimal growth conditions and in medium with modified carbon and nitrogen sources. Synthetic media with addition of complex substrates (e.g. yeast extract) and vitamin mixtures as well as some waste materials (whey, potato extract) were used as nutrient sources. Peroxide and salt stress were applied too. The production of carotene enriched biomass was carried out in flasks as well as in laboratory fermentor. The best production of biomass was obtained in inorganic medium with yeast extract. In optimal conditions tested strains differ only slightly in biomass production. All strains were able to use most of waste substrates. Biomass and pigment production was more different according to substrate type. In laboratory fermentor better producers of enriched biomass were both Rhodotorula strains. The highest yields were obtained in R. glutinis CCY 20-2-26 cells cultivated on whey medium (cca 45 g per liter of biomass enriched by 46 mg/L of beta-carotene) and in R. mucilaginosa CCY 20-7-31 grown on potato medium and 5% salt (cca 30 g per liter of biomass enriched by 56 mg/L of beta-carotene). Such dried carotenoid-enriched red yeast biomass could be directly used in feed industry as

  13. Evaluation of yeast strains for production of fuel ethanol from biomass hydrolysates

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Robust industrial yeast strains are needed for profitable production of fuel ethanol from mixed biomass waste. USDA, ARS, NCAUR, RPT has been evaluating ethanol-producing yeasts, including Saccharomyces cerevisiae, engineered GMAX Saccharomyces cerevisiae, irradiated Kluyveromyces marxianus, and Pi...

  14. Efficient recovery of nitrate and phosphate from wastewater by an amine-grafted adsorbent for cyanobacterial biomass production.

    PubMed

    Kim, Jungmin; Hwang, Min-Jin; Lee, Sang-Jun; Noh, Won; Kwon, Jung Min; Choi, Jin Soo; Kang, Chang-Min

    2016-04-01

    Various types of wastewater have been widely utilized in microalgae and cyanobacteria cultivation for environmental and economic reasons. However, the problems of low cell growth and biomass contamination due to direct use of wastewater remain unresolved. In the present study, nitrate and phosphate were separated from wastewater by adsorption and subsequently used for cyanobacterial biomass production. To this end, an amine-grafted magnetic absorbent was synthesized. The synthesized absorbent recovered ca. 78% nitrate and 93% phosphate from wastewater. Regenerated medium was prepared using recovered nutrients as nitrogen and phosphate sources, which were efficiently assimilated by cyanobacterial culture. Compared to synthetic medium, there was no difference in growth and nutrient removal using regenerated medium. The proposed indirect method of wastewater utilization would prevent contamination of the produced biomass by unfavorable substances, which will broaden its potential applications. PMID:26827169

  15. Nitrogen rates for biomass sorghum production across tillage systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biomass yields and nutrient removal across different tillage systems and nitrogen rates are not well established for forage sorghum (Sorghum bicolor L. Moench) grown as an energy source in the Southeast. An experiment was initiated in long-term conventional and conservation tillage systems on a Comp...

  16. Production and analysis of fast pyrolysis oils from proteinaceous biomass

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fast pyrolysis of lignocellulosic biomass is a facile method for producing high yields of liquid fuel intermediates. However, because most fast pyrolysis oils are highly oxygenated, acidic and unstable identification of feedstocks that produce higher quality pyrolysis liquids is desirable. Therefor...

  17. Cover crop biomass harvest for bioenergy: implications for crop productivity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Winter cover crops, such as rye (Secale cereale), are usually used in conservation agriculture systems in the Southeast. Typically, the cover crop is terminated two to three weeks before planting the summer crop, with the cover biomass left on the soil surface as a mulch. However, these cover crops ...

  18. Testing of Willow Clones for Biomass Production in Wisconsin

    SciTech Connect

    Kubiske, Marke E.

    2005-01-01

    A core experiment with 31 willow clones and 8 standard poplar clones was established at the Harshaw Experimental Farm, Rhinelander, WI in 1997. Data analysis is continuing for survival, growth, and biomass data for all willow test sites in this project.

  19. Audible sound treatment of the microalgae Picochlorum oklahomensis for enhancing biomass productivity.

    PubMed

    Cai, Weiming; Dunford, Nurhan Turgut; Wang, Ning; Zhu, Songming; He, Huinong

    2016-02-01

    It has been reported in the literature that exposure of microalgae cells to audible sound could promote growth. This study examined the effect of sound waves with the frequency of 1100 Hz, 2200 Hz, and 3300 Hz to stimulate the biomass productivity of an Oklahoma native strain, Picochlorum oklahomensis (PO). The effect of the frequency of sound on biomass mass was measured. This study demonstrated that audible sound treatment of the algae cultures at 2200 Hz was the most effective in terms of biomass production and volumetric oil yield. PMID:26716888

  20. Top Value-Added Chemicals from Biomass - Volume II—Results of Screening for Potential Candidates from Biorefinery Lignin

    SciTech Connect

    Holladay, John E.; White, James F.; Bozell, Joseph J.; Johnson, David

    2007-10-01

    This report evaluates lignin’s role as a renewable raw material resource. Opportunities that arise from utilizing lignin fit into one of three categories: 1)power, fuel and syngas (generally near-term opportunities) 2) macromolecules (generally medium-term opportunities) 3) aromatics and miscellaneous monomers (long-term opportunities). Biorefineries will receive and process massive amounts of lignin. For this reason, how lignin can be best used to support the economic health of the biorefinery must be defined. An approach that only considers process heat would be shortsighted. Higher value products present economic opportunities and the potential to significantly increase the amount of liquid transportation fuel available from biomass. In this analysis a list of potential uses of lignin was compiled and sorted into “product types” which are broad classifications (listed above as power—fuel—syngas; macromolecules; and aromatics). In the first “product type” (power—fuel—gasification) lignin is used purely as a carbon source and aggressive means are employed to break down its polymeric structure. In the second “product type” (macromolecules) the opposite extreme is considered and advantage of the macromolecular structure imparted by nature is retained in high-molecular weight applications. The third “product type” (aromatics) lies somewhere between the two extremes and employs technologies that would break up lignin’s macromolecular structure but maintain the aromatic nature of the building block molecules. The individual opportunities were evaluated based on their technical difficulty, market, market risk, building block utility, and whether a pure material or a mixture would be produced. Unlike the “Sugars Top 10” report it was difficult to identify the ten best opportunities, however, the potential opportunities fell nicely into near-, medium- and long-term opportunities. Furthermore, the near-, medium- and long-term opportunities

  1. Potentials of Biomass Co-Combustion in Coal-Fired Boilers

    NASA Astrophysics Data System (ADS)

    Werther, J.

    The present work provides a survey on the potentials of co-combustion of biomass and biogenic wastes in large-scale coal-fired power plants. This allows an energetic utilization at a high level of efficiency which is not obtainable in small-scale dedicated biomass combustors. Co-firing at low percentages of the thermal power (typically below 5-10 %) avoids the characteristic operating problems of biomass combustion, i.e. ash sintering and fouling of heat transfer surfaces. Co-firing of biogenic wastes is already widely practiced in Germany, non-waste biomass like forest residues are for subsidy reasons combusted in small dedicated mono-combustion plants. A future increase of co-combustion may be associated with the upgrading of biogenic wastes with high water content to biofuels by drying. Such biofuels could substitute more expensive coal and save on CO2 emission certificates. In the more distant future biomass co-combustion may help in the CO2 scrubbing process by lowering the target level of CO2 absorption efficiency.

  2. Preliminary investigation on the production of fuels and bio-char from Chlamydomonas reinhardtii biomass residue after bio-hydrogen production.

    PubMed

    Torri, Cristian; Samorì, Chiara; Adamiano, Alessio; Fabbri, Daniele; Faraloni, Cecilia; Torzillo, Giuseppe

    2011-09-01

    The aim of this work was to investigate the potential conversion of Chlamydomonas reinhardtii biomass harvested after hydrogen production. The spent algal biomass was converted into nitrogen-rich bio-char, biodiesel and pyrolysis oil (bio-oil). The yield of lipids (algal oil), obtained by solvent extraction, was 15 ± 2% w/w(dry-biomass). This oil was converted into biodiesel with a 8.7 ± 1% w/w(dry-biomass) yield. The extraction residue was pyrolysed in a fixed bed reactor at 350 °C obtaining bio-char as the principal fraction (44 ± 1% w/w(dry-biomass)) and 28 ± 2% w/w(dry-biomass) of bio-oil. Pyrolysis fractions were characterized by elemental analysis, while the chemical composition of bio-oil was fully characterized by GC-MS, using various derivatization techniques. Energy outputs resulting from this approach were distributed in hydrogen (40%), biodiesel (12%) and pyrolysis fractions (48%), whereas bio-char was the largest fraction in terms of mass. PMID:21345670

  3. LED light stress induced biomass and fatty acid production in microalgal biosystem, Acutodesmus obliquus

    NASA Astrophysics Data System (ADS)

    Choi, Yong-Keun; Kumaran, Rangarajulu Senthil; Jeon, Hyeon Jin; Song, Hak-Jin; Yang, Yung-Hun; Lee, Sang Hyun; Song, Kyung-Guen; Kim, Kwang Jin; Singh, Vijay; Kim, Hyung Joo

    2015-06-01

    Microbial algal system can serve as a potential source for the production of much high value bioproducts and biofuels. The quality and intensity of light are the key elements to optimize the production of algal biomass and fatty acid contents. This study presents the effect of differential LED flashing light conditions on the growth of microalgae, Acutodesmus obliquus. The induced light stress was optimized for its biomass and fatty acid content. The microalgae are exposed to various frequency of intermittent LED flashing light (blue and red lights) at three different phases in the 18 day cell growth (log, lag and stationary phase). The frequency of light flashing rate was adjusted to 120, 10, 5, 3.75, and 1 times per min. The effect of light stress on growth and fatty acids composition of A. obliquus induced an increase in algae growth and fatty acid production. Different optimal timing for light stress was subjected to elucidate the effect of light stress on algae growth and fatty acid production. The results showed an increase in the algae growth (1.2 mg/L of chl a content) under light stress condition at FT10 (flashing time, 10 times per min) from the initial day (log phase) compared with the control experiment (0.4 mg/L of chl a content). However, the total fatty acids (71 mg/g) and volumetric FAME production (9.4 ml/l) level was found to be significant under FT5 (flashing time, 5 times per min), adopting flashing light from day 10 (stationary phase). TEM studies also revealed the deposition of lipid to be largest in the 18 day old cells under flashing light (FT5) condition, representing maximum accumulation of lipids bodies (up to 770 nm diameter in particle size) occupying approximately 42% of the total area of the cell.

  4. Effects of Nitrogen and Desferal Treatments on CROTALARIA's (Crotalaria juncea Roth) Biomass Production

    NASA Astrophysics Data System (ADS)

    László Phd, M., ,, Dr.

    2009-04-01

    Presently sustainable agriculture is vital to achieving food security poverty alleviation and environmental protection because land degradation and desertification has occurred in all the world over cutting across a broad spectrum of contrasts in climate, ecosystem types, land uses and socio/economic settings. For this reason improving integrated soil fertility management is appreciationed and has become a major issue of concern on the development plant nutrition and plant production agendas. On plant nutrition level mineral macronutrients so nitrogen and chelating agents of different microelements so Desferal- deferoxamin-methansulfonic are essential for plant growth and development. Crotalari juncea L. is a well-known nutrient indicator fodder and green manure crop with a high yield potential. Field experiment was carried out on a chernozem meadow soil (Kunság- region of Hungary, Kunmadaras) in partly of experiment series (6 years) in 2001. The ploughed layer of region soils contained with about 2.6-3.4% humus and 40-42% clay, had a humus stability index of 0.9-2.5 by Márton (1997), pH (H2O) of 6.5-7.7, pH (KCl) of 5.3-6.8, y1 of 6.7-13.3. The topsoil was poorly supplied with all five macronutrients (N-NO3 1 mg 100 g-1, AL-soluble P2O5 14 mg 100 g-1, AL-K2O 36 mg 100 g-1, Ca 330 mg 100 g-1, Mg 43 mg 100 g-1) and with all four micronutrients (0.5m HNO3 soluble Cu 1 mg kg-1, Zn 1 mg kg-1, Mn 9 mg kg-1, Fe 80 mg kg-1) according to soil analysis. The groundwater depth was 2-3 m. Nitrogen x Desferal (Novartis Pharma AG Basie, Switzerland, Suiza 500mg) x Genotype (Brazíl-EMBRAPA/CNPH, Brazilia-DF, India-University of Agricultural Sciences, Bangalore) x Time experiment involved 4Nx2Dx2Gx3T=48 treatments in 3 replications giving a total of 144 plots. The N levels were 0, 100, 200 and 300 kg ha-1 year-1, and desferal levels 0 and 20 kg ha-1 year-1 with a 100 kg ha-1 year-1 P2O5 and 120 kg ha-1 year-1 K2O basic fertilisation. The plot size had an area of 4x2=8 m2 with

  5. Biomass in the manufacture of industrial products--the use of proteins and amino acids.

    PubMed

    Scott, Elinor; Peter, Francisc; Sanders, Johan

    2007-06-01

    The depletion in fossil feedstocks, increasing oil prices, and the ecological problems associated with CO2 emissions are forcing the development of alternative resources for energy, transport fuels, and chemicals: the replacement of fossil resources with CO2 neutral biomass. Allied with this, the conversion of crude oil products utilizes primary products (ethylene, etc.) and their conversion to either materials or (functional) chemicals with the aid of co-reagents such as ammonia and various process steps to introduce functionalities such as -NH2 into the simple structures of the primary products. Conversely, many products found in biomass often contain functionalities. Therefore, it is attractive to exploit this to bypass the use, and preparation of, co-reagents as well as eliminating various process steps by utilizing suitable biomass-based precursors for the production of chemicals. It is the aim of this mini-review to describe the scope of the possibilities to generate current functionalized chemical materials using amino acids from biomass instead of fossil resources, thereby taking advantage of the biomass structure in a more efficient way than solely utilizing biomass for the production of fuels or electricity. PMID:17387469

  6. Production of Gasoline and Diesel from Biomass via Fast Pyrolysis, Hydrotreating and Hydrocracking: A Design Case

    SciTech Connect

    Jones, Susanne B.; Valkenburt, Corinne; Walton, Christie W.; Elliott, Douglas C.; Holladay, Johnathan E.; Stevens, Don J.; Kinchin, Christopher; Czernik, Stefan

    2009-02-25

    The purpose of this study is to evaluate a processing pathway for converting biomass into infrastructure-compatible hydrocarbon biofuels. This design case investigates production of fast pyrolysis oil from biomass and the upgrading of that bio-oil as a means for generating infrastructure-ready renewable gasoline and diesel fuels. This study has been conducted using similar methodology and underlying basis assumptions as the previous design cases for ethanol. The overall concept and specific processing steps were selected because significant data on this approach exists in the public literature. The analysis evaluates technology that has been demonstrated at the laboratory scale or is in early stages of commercialization. The fast pyrolysis of biomass is already at an early stage of commercialization, while upgrading bio-oil to transportation fuels has only been demonstrated in the laboratory and at small engineering development scale. Advanced methods of pyrolysis, which are under development, are not evaluated in this study. These may be the subject of subsequent analysis by OBP. The plant is designed to use 2000 dry metric tons/day of hybrid poplar wood chips to produce 76 million gallons/year of gasoline and diesel. The processing steps include: 1.Feed drying and size reduction 2.Fast pyrolysis to a highly oxygenated liquid product 3.Hydrotreating of the fast pyrolysis oil to a stable hydrocarbon oil with less than 2% oxygen 4.Hydrocracking of the heavy portion of the stable hydrocarbon oil 5.Distillation of the hydrotreated and hydrocracked oil into gasoline and diesel fuel blendstocks 6. Hydrogen production to support the hydrotreater reactors. The "as received" feedstock to the pyrolysis plant will be "reactor ready". This development will likely further decrease the cost of producing the fuel. An important sensitivity is the possibility of co-locating the plant with an existing refinery. In this case, the plant consists only of the first three steps: feed

  7. Production of Gasoline and Diesel from Biomass via Fast Pyrolysis, Hydrotreating and Hydrocracking: A Design Case

    SciTech Connect

    Jones, Susanne B.; Valkenburt, Corinne; Walton, Christie W.; Elliott, Douglas C.; Holladay, Johnathan E.; Stevens, Don J.; Kinchin, Christopher; Czernik, Stefan

    2009-02-28

    The purpose of this study is to evaluate a processing pathway for converting biomass into infrastructure-compatible hydrocarbon biofuels. This design case investigates production of fast pyrolysis oil from biomass and the upgrading of that bio-oil as a means for generating infrastructure-ready renewable gasoline and diesel fuels. This study has been conducted using the same methodology and underlying basis assumptions as the previous design cases for ethanol. The overall concept and specific processing steps were selected because significant data on this approach exists in the public literature. The analysis evaluates technology that has been demonstrated at the laboratory scale or is in early stages of commercialization. The fast pyrolysis of biomass is already at an early stage of commercialization, while upgrading bio-oil to transportation fuels has only been demonstrated in the laboratory and at small engineering development scale. Advanced methods of pyrolysis, which are under development, are not evaluated in this study. These may be the subject of subsequent analysis by OBP. The plant is designed to use 2000 dry metric tons/day of hybrid poplar wood chips to produce 76 million gallons/year of gasoline and diesel. The processing steps include: 1.Feed drying and size reduction 2.Fast pyrolysis to a highly oxygenated liquid product 3.Hydrotreating of the fast pyrolysis oil to a stable hydrocarbon oil with less than 2% oxygen 4.Hydrocracking of the heavy portion of the stable hydrocarbon oil 5.Distillation of the hydrotreated and hydrocracked oil into gasoline and diesel fuel blendstocks 6. Hydrogen production to support the hydrotreater reactors. The “as received” feedstock to the pyrolysis plant will be “reactor ready.” This development will likely further decrease the cost of producing the fuel. An important sensitivity is the possibility of co-locating the plant with an existing refinery. In this case, the plant consists only of the first three steps

  8. Status of Process Development for Pyrolysis of Biomass for Liquid Fuels and Chemicals Production.

    SciTech Connect

    Elliott, Douglas C.

    2010-06-01

    Pyrolysis is one of several thermochemical conversion strategies to produce useful fuels from biomass material . The goal of fast pyrolysis is to maximize liquid product yield. Fast pyrolysis is accomplished by the thermal treatment of the biomass in an air-free environment. Very short heat up and cool-down is a requirement for fast pyrolysis. The typical residence time in the pyrolysis reactor is 1 second. In order to accomplish the fast heatup, grinding the biomass to a small particle size in the range of 1 mm is typical and pre-drying of the biomass to less than 10 weight percent moisture is considered the standard. Recovery of the product liquid, called bio-oil, is accomplished by a variety of methods all of which require a quick quench of the product vapor. A definition of fast pyrolysis bio-oil is provided for the CAS # RN 1207435-39-9 recently issued by ChemAbstracts Services.

  9. Pretreatment optimization of Sorghum pioneer biomass for bioethanol production and its scale-up.

    PubMed

    Koradiya, Manoj; Duggirala, Srinivas; Tipre, Devayani; Dave, Shailesh

    2016-01-01

    Based on one parameter at a time, saccharification of delignified sorghum biomass by 4% and 70% v/v sulfuric acid resulted in maximum 30.8 and 33.8 g% sugar production from biomass respectively. The Box Behnken Design was applied for further optimization of acid hydrolysis. As a result of the designed experiment 36.3g% sugar production was achieved when 3% v/v H2SO4 treatment given for 60 min at 180°C. The process was scaled-up to treat 2 kg of biomass. During the screening of yeast cultures, isolate C, MK-I and N were found to be potent ethanol producers from sorghum hydrolyzate. Culture MK-I was the best so used for scale up of ethanol production up to 25 L capacity, which gave a yield of 0.49 g ethanol/g sugar from hydrolyzate obtained from 2 kg of sorghum biomass. PMID:26384087

  10. Use of tamarisk as a potential feedstock for biofuel production.

    SciTech Connect

    Sun, Amy Cha-Tien; Norman, Kirsten

    2011-01-01

    This study assesses the energy and water use of saltcedar (or tamarisk) as biomass for biofuel production in a hypothetical sub-region in New Mexico. The baseline scenario consists of a rural stretch of the Middle Rio Grande River with 25% coverage of mature saltcedar that is removed and converted to biofuels. A manufacturing system life cycle consisting of harvesting, transportation, pyrolysis, and purification is constructed for calculating energy and water balances. On a dry short ton woody biomass basis, the total energy input is approximately 8.21 mmBTU/st. There is potential for 18.82 mmBTU/st of energy output from the baseline system. Of the extractable energy, approximately 61.1% consists of bio-oil, 20.3% bio-char, and 18.6% biogas. Water consumptive use by removal of tamarisk will not impact the existing rate of evapotranspiration. However, approximately 195 gal of water is needed per short ton of woody biomass for the conversion of biomass to biocrude, three-quarters of which is cooling water that can be recovered and recycled. The impact of salt presence is briefly assessed. Not accounted for in the baseline are high concentrations of Calcium, Sodium, and Sulfur ions in saltcedar woody biomass that can potentially shift the relative quantities of bio-char and bio-oil. This can be alleviated by a pre-wash step prior to the conversion step. More study is needed to account for the impact of salt presence on the overall energy and water balance.

  11. Estimating total standing herbaceous biomass production with LANDSAT MSS digital data

    NASA Technical Reports Server (NTRS)

    Richardson, A. J.; Everitt, J. H.; Wiegand, C. L. (Principal Investigator)

    1982-01-01

    Rangeland biomass data were correlated with spectral vegetation indices, derived from LANDSAT MSS data. LANDSAT data from five range and three other land use sites in Willacv and Cameron Counties were collected on October 17 and December 10, 1975, and on July 31 and September 23, 1976. The overall linear correlation of total standing herbaceous biomass with the LANDSAT derived perpendicular vegetation index was highly significant (r = 0.90**) for these four dates. The standard error of estimate was 722 kg/ha. Biomass data were recorded for two of these range sites for 8 months (March through October) during the 1976 growing season. Standing green biomass accounted for most of the increase in herbage, starting in June and ending about September and October. These results indicate that satellite data may be useful for the estimation of total standing herbaceous biomass production that could aid range managers in assessing range condition and animal carrying capacities of large and inaccessible range holdings.

  12. Spatial patterns of forest composition, successional pathways, and biomass production among landscape ecosystems of northwestern Lower Michigan

    SciTech Connect

    Host, G.E.

    1987-01-01

    Spatial patterns of forest composition, successional pathways, and biomass production were related to glacial landforms in a regional area of northwestern Lower Michigan. There were three general objectives: (1) to develop a geomorphic map of the study area, (2) to define and describe upland forest ecosystems, and (3) to study variation in species composition, successional pattern, and biomass production among landforms and ecosystems. Glacial landforms were mapped using field observation, airphoto interpretation, and topographic profile analysis. Eighty sample stands were located in upland landscape positions using a landform-based stratified random sampling design. Compositional patterns detected in multivariate analysis of floristic data were used to form ecological species groups and relate vegetation pattern to environmental factors. Chi-squared analyses showed significant patterns of species distribution related to landform. Potential successional pathways were studied by comparing seedling and sapling densities with current overstory composition. Total above ground biomass and biomass increment varied significantly among landforms and ecosystems. Variation in the composition, production, and structure of upland forests exhibits a pattern that corresponds closely to the geomorphic surface on which the forests developed.

  13. Advances in catalytic production of bio-based polyester monomer 2,5-furandicarboxylic acid derived from lignocellulosic biomass.

    PubMed

    Zhang, Junhua; Li, Junke; Tang, Yanjun; Lin, Lu; Long, Minnan

    2015-10-01

    Recently, the production and utilization of 2,5-furandicarboxylic acid (FDCA) have become a hot research topic in catalyst field and polyester industry for its special chemical structure and a wide range of raw material source. FDCA is a potential replacement for the terephthalic acid monomer used in the production of poly(ethylene terephthalate) (PET) and poly(butylene terephthalate) (PBT), which opens up a new pathway for obtaining biomass-based polyester to replace or partially replace petroleum based polyester. Here, we mainly reviewed the catalytic pathway for the synthesis of FDCA derived from lignocellulosic biomass or from the related downstream products, such as glucose, 5-hydroxymethylfurfural (HMF). Moreover, the utilization of oxidation catalysts, the reaction mechanism, the existing limitations and unsolved challenges were also elaborated in detail. Therefore, we hope this mini review provides a helpful overview and insight to readers in this exciting research area. PMID:26076643

  14. Use of orange peel extract for mixotrophic cultivation of Chlorella vulgaris: increased production of biomass and FAMEs.

    PubMed

    Park, Won-Kun; Moon, Myounghoon; Kwak, Min-Su; Jeon, Seungjib; Choi, Gang-Guk; Yang, Ji-Won; Lee, Bongsoo

    2014-11-01

    Mass cultivation of microalgae is necessary to achieve economically feasible production of microalgal biodiesel, but the high cost of nutrients is a major limitation. In this study, orange peel extract (OPE) was used as an inorganic and organic nutrient source for the cultivation of Chlorella vulgaris OW-01. Chemical composition analysis of the OPE indicated that it contains sufficient nutrients for mixotrophic cultivation of C. vulgaris OW-01. Analysis of biomass and FAME production showed that microalgae grown in OPE medium produced 3.4-times more biomass and 4.5-times more fatty acid methyl esters (FAMEs) than cells cultured in glucose-supplemented BG 11 medium (BG-G). These results suggest that growth of microalgae in an OPE-supplemented medium increases lipid production and that OPE has potential for use in the mass cultivation of microalgae. PMID:25218207

  15. Enzymatic saccharification of pretreated rice straw and biomass production

    SciTech Connect

    Araujo, A.; D'Souza, J.

    1986-10-01

    A comparative study on the saccharification of pretreated rice straw was brought about by using cellulase enzyme produced by Aspergillus terreus ATCC 52430 and its mutant strain UNGI-40. The effect of enzyme and substrate concentrations on the saccharification rate at 24 and 48 were studied. A syrup with 7% sugar concentration was obtained with a 10% substrate concentration for the mutant case, whereas a syrup with 6.8% sugar concentration was obtained with 3.5 times concentrated enzyme from the wild strain. A high saccharification value was obtained with low substrate concentration; the higher the substrate concentration used, the lower the percent saccharification. The glucose content in the hydrolysate comprised 80-82% of total reducing sugars; the remainder was cellobiose and xylose together. The hydrolysate supported the growth of yeasts Candida utilis and Saccharomyces cerevisiae ATCC 52431. A biomass with a 48% protein content was obtained. The essential amino acid composition of yeast biomass was determined.

  16. Biomass and pigments production in photosynthetic bacteria wastewater treatment: effects of light sources.

    PubMed

    Zhou, Qin; Zhang, Panyue; Zhang, Guangming

    2015-03-01

    This study is aimed at enhancing biomass and pigments production together with pollution removal in photosynthetic bacteria (PSB) wastewater treatment via different light sources. Red, yellow, blue, white LED and incandescent lamp were used. Results showed different light sources had great effects on the PSB. PSB had the highest biomass production, COD removal and biomass yield with red LED. The corresponding biomass, COD removal and biomass yield reached 2580 mg/L, 88.6% and 0.49 mg-biomass/mg-COD-removal, respectively. The hydraulic retention time of wastewater treatment could be shortened to 72 h with red LED. Mechanism analysis showed higher ATP was produced with red LED than others. Light sources could significantly affect the pigments production. The pigments productions were greatly higher with LED than incandescent lamp. Yellow LED had the highest pigments production while red LED produced the highest carotenoid/bacteriochlorophyll ratio. Considering both efficiency and energy cost, red LED was the optimal light source. PMID:25575211

  17. Biofilm biomass disruption by natural substances with potential for endodontic use.

    PubMed

    Alves, Flávio Rodrigues Ferreira; Silva, Marlei Gomes; Rôças, Isabela Neves; Siqueira, José Freitas

    2013-01-01

    This study evaluated the in vitro effects of four natural substances on the biomass of bacterial biofilms to assess their potential use as root canal irrigants. The following substances and their combinations were tested: 0.2% farnesol; 5% xylitol; 20% xylitol; 0.2% farnesol and 5% xylitol; 0.2% farnesol, 5% xylitol, and 0.1% lactoferrin; 5% xylitol and 0.1% lactoferrin; and 20 mM salicylic acid. The crystal violet assay was used to evaluate the effects of these substances on the biomass of biofilms formed by Enterococcus faecalis and Staphylococcus epidermidis. All substances except for 20 mM salicylic acid and 20% xylitol reduced biofilm mass when compared to controls. The combination of farnesol and xylitol was the most effective agent against E. faecalis ATCC 29212 (p < 0.05). Farnesol combined with xylitol and lactoferrin was the most effective against biofilms of the endodontic strain of E. faecalis MB35 (p < 0.05). Similarly, combinations involving farnesol, xylitol, and lactoferrin reduced the biomass of S. epidermidis biofilms. In general, farnesol, xylitol, and lactoferrin or farnesol and xylitol reduced biofilm biomass most effectively. Therefore, it was concluded that combinations of antibiofilm substances have potential use in endodontic treatment to combat biofilms. PMID:23306623

  18. Oyster reef restoration supports increased nekton biomass and potential commercial fishery value.

    PubMed

    Humphries, Austin T; La Peyre, Megan K

    2015-01-01

    Across the globe, discussions centered on the value of nature drive many conservation and restoration decisions. As a result, justification for management activities increasingly asks for two lines of evidence: (1) biological proof of augmented ecosystem function or service, and (2) monetary valuation of these services. For oyster reefs, which have seen significant global declines and increasing restoration work, the need to provide both biological and monetary evidence of reef services on a local-level has become more critical in a time of declining resources. Here, we quantified species biomass and potential commercial value of nekton collected from restored oyster (Crassostrea virginica) reefs in coastal Louisiana over a 3-year period, providing multiple snapshots of biomass support over time. Overall, and with little change over time, fish and invertebrate biomass is 212% greater at restored oyster reefs than mud-bottom, or 0.12 kg m(-2). The additional biomass of commercial species is equivalent to an increase of local fisheries value by 226%, or $0.09 m(-2). Understanding the ecosystem value of restoration projects, and how they interact with regional management priorities, is critical to inform local decision-making and provide testable predictions. Quantitative estimates of potential commercial fisheries enhancement by oyster reef restoration such as this one can be used directly by local managers to determine the expected return on investment. PMID:26336635

  19. Oyster reef restoration supports increased nekton biomass and potential commercial fishery value

    PubMed Central

    2015-01-01

    Across the globe, discussions centered on the value of nature drive many conservation and restoration decisions. As a result, justification for management activities increasingly asks for two lines of evidence: (1) biological proof of augmented ecosystem function or service, and (2) monetary valuation of these services. For oyster reefs, which have seen significant global declines and increasing restoration work, the need to provide both biological and monetary evidence of reef services on a local-level has become more critical in a time of declining resources. Here, we quantified species biomass and potential commercial value of nekton collected from restored oyster (Crassostrea virginica) reefs in coastal Louisiana over a 3-year period, providing multiple snapshots of biomass support over time. Overall, and with little change over time, fish and invertebrate biomass is 212% greater at restored oyster reefs than mud-bottom, or 0.12 kg m−2. The additional biomass of commercial species is equivalent to an increase of local fisheries value by 226%, or $0.09 m−2. Understanding the ecosystem value of restoration projects, and how they interact with regional management priorities, is critical to inform local decision-making and provide testable predictions. Quantitative estimates of potential commercial fisheries enhancement by oyster reef restoration such as this one can be used directly by local managers to determine the expected return on investment. PMID:26336635

  20. Methane and fertilizer production from seaweed biomass. Final report

    SciTech Connect

    Betzer, P.R.; Humm, H.J.

    1984-01-01

    It was demonstrated that several varieties of abundant benthic algae indigenous to Tampa Bay (Gracilaria, Hypnea, and Ulva) were readily degradable via anaerobic digestion to methane. The energy yield per unit weight biomass degraded was higher than any previously reported. Given the large masses of readily degradable plants which are annually produced in and around Tampa Bay, the resource is estimated to be at least equivalent to several million gallons of gasoline.

  1. Algal biomass and primary production within a temperate zone sandstone

    SciTech Connect

    Bell, R.A.; Sommerfeld, M.R. )

    1987-02-01

    The use of dimethyl sulfoxide (DMSO) to extract chlorophyll a and {sup 14}C-labelled photosynthate from endolithic algae of sparsely vegetated, cold temperate grasslands on the Colorado Plateau in Arizona has yielded the first estimates of biomass and photosynthesis for this unusual community. These subsurface microorganisms are found widespread in exposed Coconino Sandstone, a predominant formation in this cold temperate region. The endolithic community in Coconino Sandstone, composed primarily of coccoid blue-green and coccoid/sarcinoid green algae, yielded a biomass value (as chlorophyll a content) of 87 mg m{sup {minus}2} rock surface area and a photosynthetic rate of 0.37 mg CO{sub 2} dm{sup {minus}2} hr{sup {minus}1} or 0.48 mg CO{sub 2} mg{sup {minus}1} chl a hr{sup {minus}1}. The endolithic algal community contributes moderate biomass (5-10%) and substantial photosynthesis (20-80%) to the sparse grassland ecosystem.

  2. Technoeconomic analysis of different options for the production of hydrogen from sunlight, wind, and biomass

    SciTech Connect

    Mann, M.K.; Spath, P.L.; Amos, W.A.

    1998-08-01

    To determine their technical and economic viability and to provide insight into where each technology is in its development cycle, different options to produce hydrogen from sunlight, wind, and biomass were studied. Additionally, costs for storing and transporting hydrogen were determined for different hydrogen quantities and storage times. The analysis of hydrogen from sunlight examined the selling price of hydrogen from two technologies: direct photoelectrochemical (PEC) conversion of sunlight and photovoltaic (PV)-generated electricity production followed by electrolysis. The wind analysis was based on wind-generated electricity production followed by electrolysis. In addition to the base case analyses, which assume that hydrogen is the sole product, three alternative scenarios explore the economic impact of integrating the PV- and wind-based systems with the electric utility grid. Results show that PEC hydrogen production has the potential to be economically feasible. Additionally, the economics of the PV and wind electrolysis systems are improved by interaction with the grid. The analysis of hydrogen from biomass focused on three gasification technologies. The systems are: low pressure, indirectly-heated gasification followed by steam reforming; high pressure, oxygen-blown gasification followed by steam reforming; and pyrolysis followed by partial oxidation. For each of the systems studied, the downstream process steps include shift conversion followed by hydrogen purification. Only the low pressure system produces hydrogen within the range of the current industry selling prices (typically $0.7--$2/kg, or $5--14/GJ on a HHV basis). A sensitivity analysis showed that, for the other two systems, in order to bring the hydrogen selling price down to $2/kg, negative-priced feedstocks would be required.

  3. Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass.

    PubMed

    O'Neil, Gregory W; Williams, John R; Wilson-Peltier, Julia; Knothe, Gerhard; Reddy, Christopher M

    2016-01-01

    The need to replace petroleum fuels with alternatives from renewable and more environmentally sustainable sources is of growing importance. Biomass-derived biofuels have gained considerable attention in this regard, however first generation biofuels from edible crops like corn ethanol or soybean biodiesel have generally fallen out of favor. There is thus great interest in the development of methods for the production of liquid fuels from domestic and superior non-edible sources. Here we describe a detailed procedure for the production of a purified biodiesel from the marine microalgae Isochrysis. Additionally, a unique suite of lipids known as polyunsaturated long-chain alkenones are isolated in parallel as potentially valuable coproducts to offset the cost of biodiesel production. Multi-kilogram quantities of Isochrysis are purchased from two commercial sources, one as a wet paste (80% water) that is first dried prior to processing, and the other a dry milled powder (95% dry). Lipids are extracted with hexanes in a Soxhlet apparatus to produce an algal oil ("hexane algal oil") containing both traditional fats (i.e., triglycerides, 46-60% w/w) and alkenones (16-25% w/w). Saponification of the triglycerides in the algal oil allows for separation of the resulting free fatty acids (FFAs) from alkenone-containing neutral lipids. FFAs are then converted to biodiesel (i.e., fatty acid methyl esters, FAMEs) by acid-catalyzed esterification while alkenones are isolated and purified from the neutral lipids by crystallization. We demonstrate that biodiesel from both commercial Isochrysis biomasses have similar but not identical FAME profiles, characterized by elevated polyunsaturated fatty acid contents (approximately 40% w/w). Yields of biodiesel were consistently higher when starting from the Isochrysis wet paste (12% w/w vs. 7% w/w), which can be traced to lower amounts of hexane algal oil obtained from the powdered Isochrysis product. PMID:27404113

  4. Biomass Productivity Dynamics Monitoring and its Drivers in Sahelian Croplands and Rangelands to Support Food Security Policies

    NASA Astrophysics Data System (ADS)

    Leroux, L.

    2015-12-01

    Since the Sahelian population livelihood relies mainly on agropastoral activities, accurate information on biomass productivity dynamics and the underlying drivers are needed to manage a wide range of issues such as food security. This study aims to contribute to a better understanding of these drivers in rangeland and cropland, both at the Sahel and local scales (an agropastoral site in South-West Niger). At the Sahel scale, the MODIS Land Cover product was used to extract cropland and rangeland pixels. By analyzing MODIS NDVI trends together with TRMM3B43 annual rainfall (2000-2010), we developed a new classification scheme allowing to identify areas of persistent decline/improvement in biomass productivity and to separate rainfall-driven dynamics from other factors. The results showed an overall increase of productivity in the rangeland, and both an improvement and a degradation in the cropland. We found strong evidence that the increase in biomass productivity was generally linked to increasing rainfall, while the decrease could be attributed chiefly to other factors exclusively or to a combination of both climate- and human-induced factors (see the attached Figure). At the Niger site scale, biomass trends have been put in relation with a set of potential drivers via a RandomForest model, to define which were the explanatory factors of the observed trends. The factor set covered 5 categories: climate, natural constraints, demography, physical accessibility and land cover changes. We highlighted that tiger bushes areas were particularly prone to pressure due to overgrazing and overexploitation of wood, while positive trends were mainly observed near rivers and in fossil valleys where new agricultural practices might have been promoted. The approach developped here could help to delineate areas with decrease in crop and grassland production and thus to assess the vulnerability of the population, but also to target zones with good potential for planning long

  5. Biomass pretreatment

    SciTech Connect

    Hennessey, Susan Marie; Friend, Julie; Elander, Richard T; Tucker, III, Melvin P

    2013-05-21

    A method is provided for producing an improved pretreated biomass product for use in saccharification followed by fermentation to produce a target chemical that includes removal of saccharification and or fermentation inhibitors from the pretreated biomass product. Specifically, the pretreated biomass product derived from using the present method has fewer inhibitors of saccharification and/or fermentation without a loss in sugar content.

  6. Techno-Economic Analysis of Liquid Fuel Production from Woody Biomass via Hydrothermal Liquefaction (HTL) and Upgrading

    SciTech Connect

    Zhu, Yunhua; Biddy, Mary J.; Jones, Susanne B.; Elliott, Douglas C.; Schmidt, Andrew J.

    2014-09-15

    A series of experimental work was conducted to convert woody biomass to gasoline and diesel range products via hydrothermal liquefaction (HTL) and catalytic hydroprocessing. Based on the best available test data, a techno-economic analysis (TEA) was developed for a large scale woody biomass based HTL and upgrading system to evaluate the feasibility of this technology. In this system, 2000 dry metric ton per day woody biomass was assumed to be converted to bio-oil in hot compressed water and the bio-oil was hydrotreated and/or hydrocracked to produce gasoline and diesel range liquid fuel. Two cases were evaluated: a stage-of-technology (SOT) case based on the tests results, and a goal case considering potential improvements based on the SOT case. Process simulation models were developed and cost analysis was implemented based on the performance results. The major performance results included final products and co-products yields, raw materials consumption, carbon efficiency, and energy efficiency. The overall efficiency (higher heating value basis) was 52% for the SOT case and 66% for the goal case. The production cost, with a 10% internal rate of return and 2007 constant dollars, was estimated to be $1.29 /L for the SOT case and $0.74 /L for the goal case. The cost impacts of major improvements for moving from the SOT to the goal case were evaluated and the assumption of reducing the organics loss to the water phase lead to the biggest reduction in the production cost. Sensitivity analysis indicated that the final products yields had the largest impact on the production cost compared to other parameters. Plant size analysis demonstrated that the process was economically attractive if the woody biomass feed rate was over 1,500 dry tonne/day, the production cost was competitive with the then current petroleum-based gasoline price.

  7. Stimulation of commercial algal biomass production by the use of geothermal water for temperature control

    SciTech Connect

    Bedell, G.W.

    1985-01-01

    The first pilot algal biomass production project to use geothermal water for the maintenance of optimal culture temperatures in Nevada is described. The project was initiated during the fall of 1982 by TAD's Enterprises, Inc., Wabuska (near Yerington), Nevada. The facility was designed to produce Spirulina under conditions that would more than meet the requirements of the United States Food and Drug Administration for sale to the health food market. As a result, the algae were grown in large plastic bags in order to prevent contamination by extraneous organisms. Although this system has not been tuned to its optimum potential, preliminary yields obtained over most of a year indicate not only the feasibility of the project but also a stimulation of daily output yields when compared to the daily growth yields for Spirulina reported by Israel.

  8. Direct Biodiesel Production from Wet Microalgae Biomass of Chlorella pyrenoidosa through In Situ Transesterification

    PubMed Central

    Cao, Hechun; Zhang, Zhiling; Wu, Xuwen; Miao, Xiaoling

    2013-01-01

    A one-step process was applied to directly converting wet oil-bearing microalgae biomass of Chlorella pyrenoidosa containing about 90% of water into biodiesel. In order to investigate the effects of water content on biodiesel production, distilled water was added to dried microalgae biomass to form wet biomass used to produce biodiesel. The results showed that at lower temperature of 90°C, water had a negative effect on biodiesel production. The biodiesel yield decreased from 91.4% to 10.3% as water content increased from 0% to 90%. Higher temperature could compensate the negative effect. When temperature reached 150°C, there was no negative effect, and biodiesel yield was over 100%. Based on the above research, wet microalgae biomass was directly applied to biodiesel production, and the optimal conditions were investigated. Under the optimal conditions of 100 mg dry weight equivalent wet microalgae biomass, 4 mL methanol, 8 mL n-hexane, 0.5 M H2SO4, 120°C, and 180 min reaction time, the biodiesel yield reached as high as 92.5% and the FAME content was 93.2%. The results suggested that biodiesel could be effectively produced directly from wet microalgae biomass and this effort may offer the benefits of energy requirements for biodiesel production. PMID:24195081

  9. Production of biomass by Spirulina at different groundwater type. Case of Ouargla-Southeast Algeria

    NASA Astrophysics Data System (ADS)

    Saggaï, Ali; Dadamoussa, Belkheir; Djaghoubi, Afaf; Bissati, Samia

    2016-07-01

    In this paper, Spirulina platensis was cultivated to estimate the biomass production with different groundwater type in Ouargla. Growth experiments were undertaken in flasks under shelter in outdoor condition. For this, the temperature, pH and salinity value was recorded between two days of growth. Biomass concentration in the culture media was calculated by measuring the DO625. The combination of the Mioplocen water with the nutriments gave the highest values of biomass concentration with avenge of 1.78 ±0.91g/l. All the three-type water supported the growth of Spirulina that appeared as good as a culture media.

  10. Aggravated phosphorus limitation on biomass production under increasing nitrogen loading: a meta-analysis.

    PubMed

    Li, Yong; Niu, Shuli; Yu, Guirui

    2016-02-01

    Nitrogen (N) and phosphorus (P), either individually or in combination, have been demonstrated to limit biomass production in terrestrial ecosystems. Field studies have been extensively synthesized to assess global patterns of N impacts on terrestrial ecosystem processes. However, to our knowledge, no synthesis has been done so far to reveal global patterns of P impacts on terrestrial ecosystems, especially under different nitrogen (N) levels. Here, we conducted a meta-analysis of impacts of P addition, either alone or with N addition, on aboveground (AGB) and belowground biomass production (BGB), plant and soil P concentrations, and N : P ratio in terrestrial ecosystems. Overall, our meta-analysis quantitatively confirmed existing notions: (i) colimitation of N and P on biomass production and (ii) more P limitation in tropical forest than other ecosystems. More importantly, our analysis revealed new findings: (i) P limitation on biomass production was aggravated by N enrichment and (ii) plant P concentration was a better indicator of P limitation than soil P availability. Specifically, P addition increased AGB and BGB by 34% and 13%, respectively. The effect size of P addition on biomass production was larger in tropical forest than grassland, wetland, and tundra and varied with P fertilizer forms, P addition rates, or experimental durations. The P-induced increase in biomass production and plant P concentration was larger under elevated than ambient N. Our findings suggest that the global limitation of P on biomass production will become severer under increasing N fertilizer and deposition in the future. PMID:26463578

  11. Remote sensing of biomass and annual net aerial primary productivity of a salt marsh

    NASA Technical Reports Server (NTRS)

    Hardisky, M. A.; Klemas, V.; Daiber, F. C.; Roman, C. T.

    1984-01-01

    Net aerial primary productivity is the rate of storage of organic matter in above-ground plant issues exceeding the respiratory use by the plants during the period of measurement. It is pointed out that this plant tissue represents the fixed carbon available for transfer to and consumption by the heterotrophic organisms in a salt marsh or the estuary. One method of estimating annual net aerial primary productivity (NAPP) required multiple harvesting of the marsh vegetation. A rapid nondestructive remote sensing technique for estimating biomass and NAPP would, therefore, be a significant asset. The present investigation was designed to employ simple regression models, equating spectral radiance indices with Spartina alterniflora biomass to nondestructively estimate salt marsh biomass. The results of the study showed that the considered approach can be successfully used to estimate salt marsh biomass.

  12. Numerical simulation of vortex pyrolysis reactors for condensable tar production from biomass

    SciTech Connect

    Miller, R.S.; Bellan, J.

    1998-08-01

    A numerical study is performed in order to evaluate the performance and optimal operating conditions of vortex pyrolysis reactors used for condensable tar production from biomass. A detailed mathematical model of porous biomass particle pyrolysis is coupled with a compressible Reynolds stress transport model for the turbulent reactor swirling flow. An initial evaluation of particle dimensionality effects is made through comparisons of single- (1D) and multi-dimensional particle simulations and reveals that the 1D particle model results in conservative estimates for total pyrolysis conversion times and tar collection. The observed deviations are due predominantly to geometry effects while directional effects from thermal conductivity and permeability variations are relatively small. Rapid ablative particle heating rates are attributed to a mechanical fragmentation of the biomass particles that is modeled using a critical porosity for matrix breakup. Optimal thermal conditions for tar production are observed for 900 K. Effects of biomass identity, particle size distribution, and reactor geometry and scale are discussed.

  13. Genetic manipulation of lignin reduces recalcitrance and improves biomass ethanol production from switchgrass

    SciTech Connect

    Hamilton, Choo Yieng; Fu, Chunxiang; Xiao, Xirong; Ge, Yaxin; Chen, Fang; Bouton, Joseph; Foston, Marcus; Dixon, Richard A; Wang, Zeng-Yu; Mielenz, Jonathan R

    2011-01-01

    Switchgrass is a leading dedicated bioenergy feedstock because it is a native, high yielding, perennial prairie grass with broad cultivation range and low agronomic input requirements. Biomass conversion research has developed pilot scale processes for production of ethanol and other alcohols but they remain costly primarily due to the intrinsic recalcitrance of biomass. We show here that switchgrass genetic modification can produce normal plants that have reduced thermochemical and enzymatic recalcitrance. Downregulation of the switchgrass caffeic O-methyltransferase gene decreases lignin content modestly, reduces the syringyl to guaiacyl lignin monomer ratio and increases the ethanol yield by up to a third using conventional biomass fermentation processes. The downregulated lines have wild-type biomass yields but require reduced pretreatment severity and 300-400% lower cellulase dosages for equivalent product yields significantly lowering processing costs. Alternately, our modified transgenic switchgrass lines should yield significantly more fermentation chemicals per hectare under identical process conditions.

  14. NMR techniques for determination of lipid content in microalgal biomass and their use in monitoring the cultivation with biodiesel potential.

    PubMed

    Sarpal, Amarjit S; Teixeira, Cláudia M L L; Silva, Paulo Roque Martins; da Costa Monteiro, Thays Vieira; da Silva, Júlia Itacolomy; da Cunha, Valnei Smarcaro; Daroda, Romeu José

    2016-03-01

    In the present investigation, the application of NMR spectroscopic techniques was extensively used with an objective to explore the biodiesel potential of biomass cultivated on a lab scale using strains of Chlorella vulgaris and Scenedesmus ecornis. The effect of variation in the composition of culturing medium on the neutral and polar lipids productivity, and fatty acid profile of solvent extracts of microalgae biomass was studied. Determination of unsaturated fatty acid composition (C18:N = 1-3, ω3 C20:5, ω3 C22:6), polyunsaturated fatty esters (PUFEs), saturated fatty acids (SFAs), unsaturated fatty acids (UFAs), free fatty acids (FFAs), and iodine value were achieved from a single (1)H NMR spectral analysis. The results were validated by (13)C NMR and GC-MS analyses. It was demonstrated that newly developed methods based on (1)H and (13)C NMR techniques are direct, rapid, and convenient for monitoring the microalgae cultivation process for enhancement of lipid productivity and their quality aspects in the solvent extracts of microalgal biomasses without any sample treatment and prior separation compared to other methods. The fatty acid composition of algae extracts was found to be similar to vegetable and fish oils, mostly rich in C16:0, C18:N (N = 0 to 3), and n-3 omega polyunsaturated fatty acids (PUFAs). The lipid content, particularly neutral lipids, as well as most of the quality parameters were found to be medium specific by both the strains. The newly developed methods based on NMR and ultrasonic procedure developed for efficient extraction of neutral lipids are cost economic and can be an effective aid for rapid screening of algae strains for modulation of lipid productivity with desired biodiesel quality and value-added products including fatty acid profile. PMID:26615401

  15. In situ hydrogen, acetone, butanol, ethanol and microdiesel production by Clostridium acetobutylicum ATCC 824 from oleaginous fungal biomass.

    PubMed

    Hassan, Elhagag Ahmed; Abd-Alla, Mohamed Hemida; Bagy, Magdy Mohamed Khalil; Morsy, Fatthy Mohamed

    2015-08-01

    An in situ batch fermentation technique was employed for biohydrogen, acetone, butanol, ethanol and microdiesel production from oleaginous fungal biomass using the anaerobic fermentative bacterium Clostridium acetobutylicum ATCC 824. Oleaginous fungal Cunninghamella echinulata biomass which has ability to accumulate up to 71% cellular lipid was used as the substrate carbon source. The maximum cumulative hydrogen by C. acetobutylicum ATCC 824 from crude C. echinulata biomass was 260 ml H2 l(-1), hydrogen production efficiency was 0.32 mol H2 mole(-1) glucose and the hydrogen production rate was 5.2 ml H2 h(-1). Subsequently, the produced acids (acetic and butyric acids) during acidogenesis phase are re-utilized by ABE-producing clostridia and converted into acetone, butanol, and ethanol. The total ABE produced by C. acetobutylicum ATCC 824 during batch fermentation was 3.6 g l(-1) from crude fungal biomass including acetone (1.05 g l(-1)), butanol (2.19 g l(-1)) and ethanol (0.36 g l(-1)). C. acetobutylicum ATCC 824 has ability to produce lipolytic enzymes with a specific activity 5.59 U/mg protein to hydrolyze ester containing substrates. The lipolytic potential of C. acetobutylicum ATCC 824 was used as a biocatalyst for a lipase transesterification process using the produced ethanol from ABE fermentation for microdiesel production. The fatty acid ethyl esters (microdiesel) generated from the lipase transesterification of crude C. echinulata dry mass was analyzed by GC/MS as 15.4% of total FAEEs. The gross energy content of biohydrogen, acetone, butanol, ethanol and biodiesel generated through C. acetobutylicum fermentation from crude C. echinulata dry mass was 3113.14 kJ mol(-1). These results suggest a possibility of integrating biohydrogen, acetone, butanol and ethanol production technology by C. acetobutylicum with microdiesel production from crude C. echinulata dry mass and therefore improve the feasibility and commercialization of bioenergy production. PMID

  16. Influence of Plant Community Composition on Biomass Production in Planted Grasslands

    PubMed Central

    Henschell, Max A.; Webster, Christopher R.; Flaspohler, David J.; Fortin, Chad R.

    2015-01-01

    United States energy policy mandates increased use of renewable fuels. Restoring grasslands could contribute to a portion of this requirement through biomass harvest for bioenergy use. We investigated which plant community characteristics are associated with differences in biomass yield from a range of realistic native prairie plantings (n = 11; i.e., conservation planting, restoration, and wildlife cover). Our primary goal was to understand whether patterns in plant community composition and the Floristic Quality Index (FQI) were related to productivity as evidenced by dormant season biomass yield. FQI is an objective measure of how closely a plant community represents that of a pre-European settlement community. Our research was conducted in planted fields of native tallgrass prairie species, and provided a gradient in floristic quality index, species richness, species diversity, and species evenness in south-central Wisconsin during 2008 and 2009. We used a network of 15 randomly located 1 m2 plots within each field to characterize the plant community and estimate biomass yield by clipping the plots at the end of each growing season. While plant community composition and diversity varied significantly by planting type, biomass yield did not vary significantly among planting types (ANOVA; P >0.05). Biomass yield was positively correlated with plant community evenness, richness, C4 grass cover, and floristic quality index, but negatively correlated with plant species diversity in our multi-season multiple linear mixed effects models. Concordantly, plots with biomass yield in the lowest quartile (biomass yield < 3500 kh/ha) had 8% lower plant community evenness and 9% lower FQI scores than those in the upper quartile (biomass yield > 5800 kh/ha). Our results suggest that promoting the establishment of fields with high species evenness and floristic quality may increase biomass yield, while simultaneously supporting biodiversity. PMID:26018412

  17. Production of light olefins by catalytic conversion of lignocellulosic biomass with HZSM-5 zeolite impregnated with 6wt.% lanthanum.

    PubMed

    Huang, Weiwei; Gong, Feiyan; Fan, Minghui; Zhai, Qi; Hong, Chenggui; Li, Quanxin

    2012-10-01

    Catalytic conversion of rice husk, sawdust, sugarcane bagasse, cellulose, hemicellulose and lignin into olefins was performed with HZSM-5 containing 6 wt.% lanthanum. The olefins yields for different feedstocks decreased in the order: cellulose>hemicellulose>sugarcane bagasse>rice husk>sawdust>lignin. Biomass containing higher content of cellulose or hemicellulose produced more olefins than feedstocks with higher content of lignin. Among the biomass types, sugarcane bagasse provided the highest olefin yield of 0.12 kg olefins/(kg dry biomass) and carbon yield of 21.2C-mol%. Temperature, residence time and the catalyst/feed ratio influenced olefin yield and selectivity. While the HZSM-5 zeolite was catalytically active, the incorporation of lanthanum at 2.9, and 6.0 wt.% increased the production of olefins from rice husk by 15.6% and 26.5%, respectively. The conversion of biomass to light olefins potentially provides an alternative and sustainable route for production of the key petrochemicals. PMID:22858493

  18. Natural Gas and Cellulosic Biomass: A Clean Fuel Combination? Determining the Natural Gas Blending Wall in Biofuel Production.

    PubMed

    M Wright, Mark; Seifkar, Navid; Green, William H; Román-Leshkov, Yuriy

    2015-07-01

    Natural gas has the potential to increase the biofuel production output by combining gas- and biomass-to-liquids (GBTL) processes followed by naphtha and diesel fuel synthesis via Fischer-Tropsch (FT). This study reflects on the use of commercial-ready configurations of GBTL technologies and the environmental impact of enhancing biofuels with natural gas. The autothermal and steam-methane reforming processes for natural gas conversion and the gasification of biomass for FT fuel synthesis are modeled to estimate system well-to-wheel emissions and compare them to limits established by U.S. renewable fuel mandates. We show that natural gas can enhance FT biofuel production by reducing the need for water-gas shift (WGS) of biomass-derived syngas to achieve appropriate H2/CO ratios. Specifically, fuel yields are increased from less than 60 gallons per ton to over 100 gallons per ton with increasing natural gas input. However, GBTL facilities would need to limit natural gas use to less than 19.1% on a LHV energy basis (7.83 wt %) to avoid exceeding the emissions limits established by the Renewable Fuels Standard (RFS2) for clean, advanced biofuels. This effectively constitutes a blending limit that constrains the use of natural gas for enhancing the biomass-to-liquids (BTL) process. PMID:26010031

  19. Anaerobic Fungi and Their Potential for Biogas Production.

    PubMed

    Dollhofer, Veronika; Podmirseg, Sabine Marie; Callaghan, Tony Martin; Griffith, Gareth Wyn; Fliegerová, Kateřina

    2015-01-01

    Plant biomass is the largest reservoir of environmentally friendly renewable energy on earth. However, the complex and recalcitrant structure of these lignocellulose-rich substrates is a severe limitation for biogas production. Microbial pro-ventricular anaerobic digestion of ruminants can serve as a model for improvement of converting lignocellulosic biomass into energy. Anaerobic fungi are key players in the digestive system of various animals, they produce a plethora of plant carbohydrate hydrolysing enzymes. Combined with the invasive growth of their rhizoid system their contribution to cell wall polysaccharide decomposition may greatly exceed that of bacteria. The cellulolytic arsenal of anaerobic fungi consists of both secreted enzymes, as well as extracellular multi-enzyme complexes called cellulosomes. These complexes are extremely active, can degrade both amorphous and crystalline cellulose and are probably the main reason of cellulolytic efficiency of anaerobic fungi. The synergistic use of mechanical and enzymatic degradation makes anaerobic fungi promising candidates to improve biogas production from recalcitrant biomass. This chapter presents an overview about their biology and their potential for implementation in the biogas process. PMID:26337843

  20. Pyrolysis of wetland biomass waste: Potential for carbon sequestration and water remediation.

    PubMed

    Cui, Xiaoqiang; Hao, Hulin; He, Zhenli; Stoffella, Peter J; Yang, Xiaoe

    2016-05-15

    Management of biomass waste is crucial to the efficiency and sustainable operation of constructed wetlands. In this study, biochars were prepared using the biomass of 22 plant species from constructed wetlands and characterized by BET-N2 surface area analysis, FTIR, TGA, SEM, EDS, and elemental compositions analysis. Biochar yields ranged from 32.78 to 49.02%, with mesopores dominating the pore structure of most biochars. The biochars had a R50 recalcitrance index of class C and the carbon sequestration potential of 19.4-28%. The aquatic plant biomass from all the Chinese constructed wetlands if made into biochars has the potential to sequester 11.48 Mt carbon yr(-1) in soils over long time periods, which could offset 0.4% of annual CO2 emissions from fossil fuel combustion in China. In terms of adsorption capacity for selected pollutants, biochar derived from Canna indica plant had the greatest adsorption capacity for Cd(2+) (98.55 mg g(-1)) and NH4(+) (7.71 mg g(-1)). Whereas for PO4(3-), Hydrocotyle verticillata derived biochar showed the greatest adsorption capacities (2.91 mg g(-1)). The results from this present study demonstrated that wetland plants are valuable feedstocks for producing biochars with potential application for carbon sequestration and contaminant removal in water remediation. PMID:26978731

  1. RENEWABLE ENERGY AND ENVIRONMENTAL SUSTAINABILITY USING BIOMASS FROM DAIRY AND BEEF ANIMAL PRODUCTION

    SciTech Connect

    Sweeten, John; Annamalai, Kalyan; Auvermann, Brent; Mukhtar, Saqib; Capareda, Sergio C; Engler, Cady; Harman, Wyatte; Reddy, J N; DeOtte, Robert; Parker, David B; Stewart, B A

    2012-05-02

    The Texas Panhandle is regarded as the "Cattle Feeding Capital of the World", producing 42% of the fed beef cattle in the United States within a 200-mile radius of Amarillo generating more than 5 million tons of feedlot manure /year. Apart from feedlots, the Bosque River Region in Erath County, just north of Waco, Texas with about 110,000 dairy cattle in over 250 dairies, produces 1.8 million tons of manure biomass (excreted plus bedding) per year. While the feedlot manure has been used extensively for irrigated and dry land crop production, most dairies, as well as other concentrated animal feeding operations (CAFO's), the dairy farms utilize large lagoon areas to store wet animal biomass. Water runoff from these lagoons has been held responsible for the increased concentration of phosphorus and other contaminates in the Bosque River which drains into Lake Waco—the primary source of potable water for Waco's 108,500 people. The concentrated animal feeding operations may lead to land, water, and air pollution if waste handling systems and storage and treatment structures are not properly managed. Manure-based biomass (MBB) has the potential to be a source of green energy at large coal-fired power plants and on smaller-scale combustion systems at or near confined animal feeding operations. Although MBB particularly cattle biomass (CB) is a low quality fuel with an inferior heat value compared to coal and other fossil fuels, the concentration of it at large animal feeding operations can make it a viable source of fuel. The overall objective of this interdisciplinary proposal is to develop environmentally benign technologies to convert low-value inventories of dairy and beef cattle biomass into renewable energy. Current research expands the suite of technologies by which cattle biomass (CB: manure, and premature mortalities) could serve as a renewable alternative to fossil fuel. The work falls into two broad categories of research and development. Category 1

  2. RENEWABLE ENERGY AND ENVIRONMENTAL SUSTAINABILITY USING BIOMASS FROM DAIRY AND BEEF ANIMAL PRODUCTION

    SciTech Connect

    John M. Sweeten, Kalyan Annamalai Brent Auvermann Saqib Mukhtar Sergio C. Capareda Cady Engler Wyatte Harman J.N. Reddy, Robert DeOtte David B. Parker Dr. B.A. Stewart

    2012-05-03

    The Texas Panhandle is regarded as the 'Cattle Feeding Capital of the World', producing 42% of the fed beef cattle in the United States within a 200-mile radius of Amarillo generating more than 5 million tons of feedlot manure/year. Apart from feedlots, the Bosque River Region in Erath County, just north of Waco, Texas with about 110,000 dairy cattle in over 250 dairies, produces 1.8 million tons of manure biomass (excreted plus bedding) per year. While the feedlot manure has been used extensively for irrigated and dry land crop production, most dairies, as well as other concentrated animal feeding operations (CAFO's), the dairy farms utilize large lagoon areas to store wet animal biomass. Water runoff from these lagoons has been held responsible for the increased concentration of phosphorus and other contaminates in the Bosque River which drains into Lake Waco - the primary source of potable water for Waco's 108,500 people. The concentrated animal feeding operations may lead to land, water, and air pollution if waste handling systems and storage and treatment structures are not properly managed. Manure-based biomass (MBB) has the potential to be a source of green energy at large coal-fired power plants and on smaller-scale combustion systems at or near confined animal feeding operations. Although MBB particularly cattle biomass (CB) is a low quality fuel with an inferior heat value compared to coal and other fossil fuels, the concentration of it at large animal feeding operations can make it a viable source of fuel. The overall objective of this interdisciplinary proposal is to develop environmentally benign technologies to convert low-value inventories of dairy and beef cattle biomass into renewable energy. Current research expands the suite of technologies by which cattle biomass (CB: manure, and premature mortalities) could serve as a renewable alternative to fossil fuel. The work falls into two broad categories of research and development. Category 1

  3. RENEWABLE ENERGY AND ENVIRONMENTAL SUSTAINABILITY USING BIOMASS FROM DAIRY AND BEEF ANIMAL PRODUCTION

    SciTech Connect

    Sweeten, John M; Annamalai, Kalyan; Auvermann, Brent; Mukhtar, Saqib; Capareda, Sergio C.; Engler, Cady; Harman, Wyatte; Reddy, J N; DeOtte, Robert; Parker, David B.; Stewart, B. A.

    2012-05-03

    The Texas Panhandle is regarded as the "Cattle Feeding Capital of the World", producing 42% of the fed beef cattle in the United States within a 200-mile radius of Amarillo generating more than 5 million tons of feedlot manure/year. Apart from feedlots, the Bosque River Region in Erath County, just north of Waco, Texas with about 110,000 dairy cattle in over 250 dairies, produces 1.8 million tons of manure biomass (excreted plus bedding) per year. While the feedlot manure has been used extensively for irrigated and dry land crop production, most dairies, as well as other concentrated animal feeding operations (CAFO's), the dairy farms utilize large lagoon areas to store wet animal biomass. Water runoff from these lagoons has been held responsible for the increased concentration of phosphorus and other contaminates in the Bosque River which drains into Lake Waco -- the primary source of potable water for Waco's 108,500 people. The concentrated animal feeding operations may lead to land, water, and air pollution if waste handling systems and storage and treatment structures are not properly managed. Manure-based biomass (MBB) has the potential to be a source of green energy at large coal-fired power plants and on smaller-scale combustion systems at or near confined animal feeding operations. Although MBB particularly cattle biomass (CB) is a low quality fuel with an inferior heat value compared to coal and other fossil fuels, the concentration of it at large animal feeding operations can make it a viable source of fuel. The overall objective of this interdisciplinary proposal is to develop environmentally benign technologies to convert low-value inventories of dairy and beef cattle biomass into renewable energy. Current research expands the suite of technologies by which cattle biomass (CB: manure, and premature mortalities) could serve as a renewable alternative to fossil fuel. The work falls into two broad categories of research and development. Category 1

  4. 90% Below 10m: Summer Biomass and Productivity are Invisible to Satellites and Surface Transects in Modern Lake Michigan

    NASA Astrophysics Data System (ADS)

    Cuhel, R. L.; Aguilar, C.

    2013-12-01

    Deep biomass maxima, often identified through in vivo chlorophyll fluorescence profiles (DCM or deep chlorophyll maximum), have been common 'forever' in Lake Michigan. Usually present in the upper thermocline zone of 15-25m, summer DCM populations were characteristically dominated by diatoms. Increased light transmission in quagga mussel (QM) engineered Lake Michigan waters now has enabled phytoplankton to proliferate in discrete layers as deep as 50m. Instances of multiple fluorescence maxima and transmission minima, often not coincident, document the habitat diversity available in clear, often sequentially stratified offshore waters and MidLake Reef Complex locations. Phytoplankton population structure has also changed, and diatoms have become a much smaller component of algal biomass. Discrete layers of chromatically adapted picoplankton now dominate the deepest biomass maxima. Photosynthetic characteristics differ substantially among leading edge, principal biomass or fluorescence, and deep trailing edge populations. Saturation coefficients are often as low as 25 uEin/m2/sec, or 1% of midday summer surface radiance. In vivo fluorescence is only loosely related to biomass, which is greatest in shallower zones of beam transmission minima. On a daily basis, areal primary productivity post-QM is less than half of previous levels, and seasonality has been muted. Spring bloom enhancement no longer exists, and the depth zone of maximum productivity is 10-20m deeper than during the diatom epoch. Altered phytoplankton community structure and decreased productivity left strong signals in biogeochemical time series measurements. A clear discontinuity in silicate cycling indicates dampened diatom productivity and consequently lower silica loss through deposition and burial. Porewater analysis pre- and post-QM shows evidence of reduced organic sedimentation overall, with an especially strong signal in decreased potential silicate efflux. Biogeochemical consequences include

  5. Biomass and production of amphipods in low alkalinity lakes affected by acid precipitation.

    PubMed

    France, R L

    1996-01-01

    Population biomass and production of the amphipod Hyalella azteca (Saussure) were found to be related to alkalinity (ranging from 0.2 to 58.1 mg liter(-1)) in 10 Canadian Shield lakes in south-central Ontario. Biomass and production of amphipods in the two lakes characterized by spring depressions of pH below 5.0 were found to be lower than those for populations inhabiting lakes that did not experience such acid pulses. The proportional biomass of amphipods in relation to the total littoral zoobenthos community was lower in lakes of low alkalinity than in circumneutral or hardwater lakes. Because production in these amphipod populations is known to depend closely on population abundance, the labour-intensive derivation of production rates yields relatively little information for biomonitoring that cannot be obtained from abundance data alone. PMID:15093505

  6. Mixed plantations of eucalyptus and leguminous trees enhance biomass production. Forest Service research paper (Final)

    SciTech Connect

    DeBell, D.S.; Whitesell, C.D.; Schubert, T.H.

    1985-07-01

    Two Eucalyptus species--E. Saligna and E. grandis--are especially favored in Hawaii for wood, fiber, and fuel production because of their quick growth and high yields. Their growth is limited, however, on many sites by low levels of available nitrogen. Supplemental nitrogen can be provided by nitrogen-fixing plants, such as legumes. A test was conducted to determine whether planting two leguminous species--Acacia melanoxylon and Albizia falcataria Fosberg--could increase biomass production. Total biomass production was much greater in the mixed-species plantations than in the pure Eucalyptus plantation.

  7. Life cycle water footprint of hydrogenation-derived renewable diesel production from lignocellulosic biomass.

    PubMed

    Wong, Alain; Zhang, Hao; Kumar, Amit

    2016-10-01

    The conversion of lignocellulosic biomass to biofuel requires water. This study is focused on the production of hydrogenation-derived renewable diesel (HDRD) from lignocellulosic biomass. Although there has been considerable focus on the assessment of greenhouse gas (GHG) emissions, there is limited work on the assessment of the life cycle water footprint of HDRD production. This paper presents a life cycle water consumption study on lignocellulosic biomass to HDRD via pyrolysis and hydrothermal liquefaction (HTL) processes. The results of this study show that whole tree (i.e., tree chips) biomass has water requirements of 497.79 L/MJ HDRD and 376.16 L/MJ HDRD for production through fast pyrolysis and the HTL process, respectively. Forest residues (i.e., chips from branches and tops generated during logging operations) have water requirements of 338.58 L/MJ HDRD and 255.85 L/MJ HDRD for production through fast pyrolysis and the HTL process, respectively. Agricultural residues (i.e., straw from wheat, oats, and barley), which are more water efficient, have water requirements of 83.7 L/MJ HDRD and 59.1 L/MJ HDRD through fast pyrolysis and the HTL process, respectively. Differences in water use between feedstocks and conversion processes indicate that the choices of biomass feedstock and conversion pathway water efficiency are crucial factors affecting water use efficiency of HDRD production. PMID:27379729

  8. Woody biomass production lags stem-girth increase by over one month in coniferous forests.

    PubMed

    Cuny, Henri E; Rathgeber, Cyrille B K; Frank, David; Fonti, Patrick; Mäkinen, Harri; Prislan, Peter; Rossi, Sergio; Del Castillo, Edurne Martinez; Campelo, Filipe; Vavrčík, Hanuš; Camarero, Jesus Julio; Bryukhanova, Marina V; Jyske, Tuula; Gričar, Jožica; Gryc, Vladimír; De Luis, Martin; Vieira, Joana; Čufar, Katarina; Kirdyanov, Alexander V; Oberhuber, Walter; Treml, Vaclav; Huang, Jian-Guo; Li, Xiaoxia; Swidrak, Irene; Deslauriers, Annie; Liang, Eryuan; Nöjd, Pekka; Gruber, Andreas; Nabais, Cristina; Morin, Hubert; Krause, Cornelia; King, Gregory; Fournier, Meriem

    2015-01-01

    Wood is the main terrestrial biotic reservoir for long-term carbon sequestration(1), and its formation in trees consumes around 15% of anthropogenic carbon dioxide emissions each year(2). However, the seasonal dynamics of woody biomass production cannot be quantified from eddy covariance or satellite observations. As such, our understanding of this key carbon cycle component, and its sensitivity to climate, remains limited. Here, we present high-resolution cellular based measurements of wood formation dynamics in three coniferous forest sites in northeastern France, performed over a period of 3 years. We show that stem woody biomass production lags behind stem-girth increase by over 1 month. We also analyse more general phenological observations of xylem tissue formation in Northern Hemisphere forests and find similar time lags in boreal, temperate, subalpine and Mediterranean forests. These time lags question the extension of the equivalence between stem size increase and woody biomass production to intra-annual time scales(3, 4, 5, 6). They also suggest that these two growth processes exhibit differential sensitivities to local environmental conditions. Indeed, in the well-watered French sites the seasonal dynamics of stem-girth increase matched the photoperiod cycle, whereas those of woody biomass production closely followed the seasonal course of temperature. We suggest that forecasted changes in the annual cycle of climatic factors(7) may shift the phase timing of stem size increase and woody biomass production in the future. PMID:27251531

  9. Eutrophication effects on phytoplankton size-fractioned biomass and production at a tropical estuary.

    PubMed

    Guenther, Mariana; Araújo, Moacyr; Flores-Montes, Manuel; Gonzalez-Rodriguez, Eliane; Neumann-Leitão, Sigrid

    2015-02-28

    Size-fractioned phytoplankton (pico, nano and microplankton) biomass and production were estimated throughout a year at Recife harbor (NE Brazil), a shallow well mixed tropical hypereutrophic estuary with short residence times but restricted water renewal. Intense loads of P-PO4 (maximum 14 μM) resulted in low N:P ratios (around 2:1), high phytoplankton biomass (B=7.1-72 μg chl-a L(-1)), production (PP=10-2657 μg C L(-1) h(-1)) and photosynthetic efficiency (P(B)=0.5-45 μg C μg chl-a(-1)), but no oxygen depletion (average O2 saturation: 109.6%). Nanoplankton dominated phytoplankton biomass (66%) but micro- and nanoplankton performed equivalent primary production rates (47% each). Production-biomass models indicate an export of the exceeding microplankton biomass during most of the year, possibly through grazing. The intense and constant nutrient and organic matter loading at Recife harbor is thus supporting the high microplankton productivity that is not accumulating on the system nor contributing to oxygen depletion, but supporting the whole system's trophic web. PMID:25444618

  10. Short leaf mutation and modified plant architecture as potential traits for improving biomass and abiotic stress tolerance in sorghum

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The significant contributions of plant architecture to yield and biomass production have been the focus of attention in a number of crop plants. Recently, the relationship between plant architecture, biomass characteristics and responses to abiotic stresses has also been a subject of considerable in...

  11. Low-cost production of green microalga Botryococcus braunii biomass with high lipid content through mixotrophic and photoautotrophic cultivation.

    PubMed

    Yeesang, Chittra; Cheirsilp, Benjamas

    2014-09-01

    Botryococcus braunii is a microalga that is regarded as a potential source of renewable fuel because of its ability to produce large amounts of lipid that can be converted into biodiesel. Agro-industrial by-products and wastes are of great interest as cultivation medium for microorganisms because of their low cost, renewable nature, and abundance. In this study, two strategies for low-cost production of B. braunii biomass with high lipid content were performed: (i) the mixotrophic cultivation using molasses, a cheap by-product from the sugar cane plant as a carbon source, and (ii) the photoautotrophic cultivation using nitrate-rich wastewater supplemented with CO2 as a carbon source. The mixotrophic cultivation added with 15 g L(-1) molasses produced a high amount of biomass of 3.05 g L(-1) with a high lipid content of 36.9 %. The photoautotrophic cultivation in nitrate-rich wastewater supplemented with 2.0 % CO2 produced a biomass of 2.26 g L(-1) and a lipid content of 30.3 %. The benefits of this photoautotrophic cultivation are that this cultivation would help to reduce accumulation of atmospheric carbon dioxide and more than 90 % of the nitrate could be removed from the wastewater. When this cultivation was scaled up in a stirred tank photobioreactor and run with semi-continuous cultivation regime, the highest microalgal biomass of 5.16 g L(-1) with a comparable lipid content of 32.2 % was achieved. These two strategies could be promising ways for producing cheap lipid-rich microalgal biomass that can be used as biofuel feedstocks and animal feeds. PMID:24989454

  12. Energy biomass tree seedling production study. Fuels from woody biomass. Progress report, September 1978-January 1980

    SciTech Connect

    Foote, K.R.

    1980-03-01

    The research to date has centered around the establishment of baseline growing conditions for a number of species of tree seedlings, primarily deciduous hardwoods. As these baseline conditions were established for each specie, the shoot and root environments were manipulated in an attempt to establish techniques to increase seedling growth and reduce production times. Seedlings were outplanted in an attempt to establish baseline survival rates for seedlings grown in totally controlled environments. Studies to determine the optimum container for tree seedling production have been run and will continue as other containers are identified and made available. The most significant of the research results has been in the maximization of seedling growth. Seedling production times have been decreased in some species by as much as 50% under the baseline production times. Controlled environment production techniques provide for plant densities as high as 144 seedlings per square foot of growing space. Investigations of growing media indicate a significant species specific responses. Preliminary results of outplanting indicate survival rates as high as 90% plus.

  13. Potential of cyanobacterial biofilms in phosphate removal and biomass production.

    PubMed

    Rai, Jyoti; Kumar, Dhananjay; Pandey, Lalit K; Yadav, Arpana; Gaur, J P

    2016-07-15

    Four cyanobacterial biofilms, raised from cyanobacterial mats and dominated by Phormidium and Oscillatoria spp., were successfully grown attached to polyester mesh discs, and were tested for their probable application in [Formula: see text] -P removal from domestic sewage and other nutrient enriched wastewaters. Biofilm # 2, dominated by Phormidium fragile, best removed [Formula: see text] -P; nevertheless, some of it also grew outside the substrate making harvesting difficult. Other biofilms also efficiently removed [Formula: see text] -P from the medium in the following order: Biofilm # 1 > Biofilm # 3 > Biofilm # 4. Their growths were restricted to discs and are therefore better candidates as they can be efficiently harvested after [Formula: see text] -P removal. [Formula: see text] -P removal was primarily due to its uptake during growth of the biofilm rather than because of precipitation as pH of the medium remained <8.5. [Formula: see text] -N concentration in the medium determined [Formula: see text] -P removal efficiency of the test biofilms and therefore optimum N:P ratio is necessary for optimizing [Formula: see text] -P removal. The test biofilms could also efficiently remove [Formula: see text] -N from the medium. PMID:27088210

  14. DEMONSTRATION OF THE VIABILITY AND EVALUATION OF PRODUCTION COSTS FOR BIOMASS-INFUSED COAL BRIQUETTES

    SciTech Connect

    Kamshad, Kourosh

    2013-12-31

    This report is the final reporting installment of the DOE project titled DEMONSTRATION OF THE VIABILITY AND EVALUATION OF PRODUCTION COSTS FOR BIOMASS-INFUSED COAL BRIQUETTES. This rerport includes a summary of the work completed to date including the experimental methods used to acheive the results, discussions, conclusions and implications of the final product delivered by the project.

  15. A Multiple Species Approach to Biomass Production from Native Herbaceous Perennial Feedstocks

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Recent mandates to produce biofuels from cellulosic materials as well as global population increase will demand an increase of the agricultural production. Here we defend the need to produce lignocellulosic biomass in lands that are not well suited for conventional crop production, here thereafter r...

  16. Factors governing phytoplankton biomass and production in tropical estuaries of western Taiwan

    NASA Astrophysics Data System (ADS)

    Pan, Ching-Wen; Chuang, Yi-Li; Chou, Lien-Siang; Chen, Meng-Hsien; Lin, Hsing-Juh

    2016-04-01

    Factors governing phytoplankton community composition and production in tropical estuaries remain mostly unknown. We aimed to quantify phytoplankton biomass, production, and community composition seasonally in 2 tropical estuaries with different levels of nutrient concentrations and turbidity, and we compared them with an offshore control site on the western coast of central Taiwan for two years. Phytoplankton biomass and production varied with season and site. Annual integrated primary production showed that these three sites were mesotrophic systems. Spearman rank correlations showed that phytoplankton biomass and production were positively correlated with water temperature, but negatively correlated with turbidity. The threshold of turbidity was 12 Nephelometric Turbidity Units (NTU), above which phytoplankton chlorophyll a concentrations were <0.5 mg m-3, and gross production rate was <100 mg C m-3 d-1. The results of nonmetric multidimensional scaling (MDS) showed that the community was primarily structured by season and secondarily by site. The functional traits further showed that turbidity, water temperature, and SiO2 concentration were governing factors for the variations in the community. In summary, turbidity was the main factor governing phytoplankton biomass and production, whereas water temperature and SiO2 concentration had both a direct effect on production and an indirect effect by changing community composition.

  17. Potential consequences of climate change for primary production and fish production in large marine ecosystems

    PubMed Central

    Blanchard, Julia L.; Jennings, Simon; Holmes, Robert; Harle, James; Merino, Gorka; Allen, J. Icarus; Holt, Jason; Dulvy, Nicholas K.; Barange, Manuel

    2012-01-01

    Existing methods to predict the effects of climate change on the biomass and production of marine communities are predicated on modelling the interactions and dynamics of individual species, a very challenging approach when interactions and distributions are changing and little is known about the ecological mechanisms driving the responses of many species. An informative parallel approach is to develop size-based methods. These capture the properties of food webs that describe energy flux and production at a particular size, independent of species' ecology. We couple a physical–biogeochemical model with a dynamic, size-based food web model to predict the future effects of climate change on fish biomass and production in 11 large regional shelf seas, with and without fishing effects. Changes in potential fish production are shown to most strongly mirror changes in phytoplankton production. We project declines of 30–60% in potential fish production across some important areas of tropical shelf and upwelling seas, most notably in the eastern Indo-Pacific, the northern Humboldt and the North Canary Current. Conversely, in some areas of the high latitude shelf seas, the production of pelagic predators was projected to increase by 28–89%. PMID:23007086

  18. Production of Diethyl Terephthalate from Biomass-Derived Muconic Acid.

    PubMed

    Lu, Rui; Lu, Fang; Chen, Jiazhi; Yu, Weiqiang; Huang, Qianqian; Zhang, Junjie; Xu, Jie

    2016-01-01

    We report a cascade synthetic route to directly obtain diethyl terephthalate, a replacement for terephthalic acid, from biomass-derived muconic acid, ethanol, and ethylene. The process involves two steps: First, a substituted cyclohexene system is built through esterification and Diels-Alder reaction; then, a dehydrogenation reaction provides diethyl terephthalate. The key esterification reaction leads to improved solubility and modulates the electronic properties of muconic acid, thus promoting the Diels-Alder reaction with ethylene. With silicotungstic acid as the catalyst, nearly 100% conversion of muconic acid was achieved, and the cycloadducts were formed with more than 99.0% selectivity. The palladium-catalyzed dehydrogenation reaction preferentially occurs under neutral or mildly basic conditions. The total yield of diethyl terephthalate reached 80.6% based on the amount of muconic acid used in the two-step synthetic process. PMID:26592149

  19. Bio-oil production from biomass via supercritical fluid extraction

    NASA Astrophysics Data System (ADS)

    Durak, Halil

    2016-04-01

    Supercritical fluid extraction is used for producing bio-fuel from biomass. Supercritical fluid extraction process under supercritical conditions is the thermally disruption process of the lignocellulose or other organic materials at 250-400 °C temperature range under high pressure (4-5 MPa). Supercritical fluid extraction trials were performed in a cylindrical reactor (75 mL) in organic solvents (acetone, ethanol) under supercritical conditions with (calcium hydroxide, sodium carbonate) and without catalyst at the temperatures of 250, 275 and 300 °C. The produced liquids at 300 °C in supercritical liquefaction were analyzed and characterized by elemental, GC-MS and FT-IR. 36 and 37 different types of compounds were identified by GC-MS obtained in acetone and ethanol respectively.

  20. Utilisation of biomass gasification by-products for onsite energy production.

    PubMed

    Vakalis, S; Sotiropoulos, A; Moustakas, K; Malamis, D; Baratieri, M

    2016-06-01

    Small scale biomass gasification is a sector with growth and increasing applications owing to the environmental goals of the European Union and the incentivised policies of most European countries. This study addresses two aspects, which are at the centre of attention concerning the operation and development of small scale gasifiers; reuse of waste and increase of energy efficiency. Several authors have denoted that the low electrical efficiency of these systems is the main barrier for further commercial development. In addition, gasification has several by-products that have no further use and are discarded as waste. In the framework of this manuscript, a secondary reactor is introduced and modelled. The main operating principle is the utilisation of char and flue gases for further energy production. These by-products are reformed into secondary producer gas by means of a secondary reactor. In addition, a set of heat exchangers capture the waste heat and optimise the process. This case study is modelled in a MATLAB-Cantera environment. The model is non-stoichiometric and applies the Gibbs minimisation principle. The simulations show that some of the thermal energy is depleted during the process owing to the preheating of flue gases. Nonetheless, the addition of a secondary reactor results in an increase of the electrical power production efficiency and the combined heat and power (CHP) efficiency. PMID:27118736

  1. Complex analysis of energy production technologies from solid biomass in the Ukraine

    NASA Astrophysics Data System (ADS)

    Zheliezna, T. A.; Drozdova, O. I.

    2014-04-01

    The results of the energetic, economic, and environmental analyses of technologies of energy production from solid biomass are considered. Examples of the introduction of the technology of the direct combustion of biomass (straw and wood) in a boiler installation, a domestic boiler, and a combined heat and power plant (CHPP) are considered. The results indicate the energetic and environmental reasonability of implementation of such projects. From the economic viewpoint, the introduction of the boilers that use the biomass is profitable with the substitution of natural gas for the state-financed and industrial consumers, and the CHPP operation with the use of biomass is profitable with selling the electrical energy by the "feed-in" tariff.

  2. Challenges for the production of bioethanol from biomass using recombinant yeasts.

    PubMed

    Kricka, William; Fitzpatrick, James; Bond, Ursula

    2015-01-01

    Lignocellulose biomass, one of the most abundant renewable resources on the planet, is an alternative sustainable energy source for the production of second-generation biofuels. Energy in the form of simple or complex carbohydrates can be extracted from lignocellulose biomass and fermented by microorganisms to produce bioethanol. Despite 40 years of active and cutting-edge research invested into the development of technologies to produce bioethanol from lignocellulosic biomass, the process remains commercially unviable. This review describes the achievements that have been made in generating microorganisms capable of utilizing both simple and complex sugars from lignocellulose biomass and the fermentation of these sugars into ethanol. We also provide a discussion on the current "roadblocks" standing in the way of making second-generation bioethanol a commercially viable alternative to fossil fuels. PMID:26003934

  3. Phytoplankton biomass, production and growth limitations on the Huanghe (Yellow River) continental shelf

    NASA Astrophysics Data System (ADS)

    Turner, R. Eugene; Rabalais, Nancy N.; Zhang, Zhi Nan

    1990-06-01

    We examined phytoplankton populations in coastal waters of the Huanghe (Yellow River) estuary during two cruises in the annual high and normal discharge periods: August 1986 and October 1987, respectively. Strong salinity, nutrient, and phytoplankton pigment concentration gradients occur along the 5 m isobath. Landward of these gradients the phytoplankton growth potential (PGP) appears strongly phosphorus-limited and light limitation of PGP, paradoxically, appears less significant than it does further offshore where the euphotic zone depth is greater. Phytoplankton pigments are sparse both in the river and far offshore, and the peak accumulation (9 μg l -1 chlorophyll a) is centered broadly between 20 and 25 ppt, thus straddling the region of the hypopycnal plume from the hyperpycnal plunge point to where Secchi disk depth exceeds 1 m. As the suspended matter falls out (sharply) near the 25 ppt isohaline, light conditions improve, the N:P ratio drops to below 100, and nitrate concentrations continue to decrease in an offshore direction. Phytoplankton production rates reach a maximum and large algal cells accumulate where the suspended particulate matter concentration drops to less than 10 mg l -1. Both phytoplankton biomass and production declines beyond approximately 32 ppt. Sedimentary pigment accumulations also increase going from land to sea. Phosphorus and nitrogen dominate the suite of nutrients tested to determine which nutrients limit PGP. Phosphorus is probably the major nutrient limiting phytoplankton growth (not necessarily biomass accumulation) in most of the Huanghe estuary. This conclusion is based on the very high N:P ratios of dissolved nutrients, the results of an extensive array of addition and deletion bioassay experiments, and the results of P addition experiments. Where trace metals and EDTA limit PGP, they are usually limiting in concert with other nutrients and do not act alone. Comparison with other large river plumes are made.

  4. Effect of industrial waste products on phosphorus mobilisation and biomass production in abattoir wastewater irrigated soil.

    PubMed

    Seshadri, Balaji; Kunhikrishnan, Anitha; Bolan, Nanthi; Naidu, Ravi

    2014-09-01

    This study evaluated the effect of alkaline industrial by-products such as flyash (FA) and redmud (RM) on phosphorus (P) mobilisation in abattoir wastewater irrigated soils, using incubation, leaching and plant growth (Napier grass [Pennisetum purpureum]) experiments. The soil outside the wastewater irrigated area was also collected and treated with inorganic (KH2PO4 [PP]) and organic (poultry manure [PM]) P treatments, to study the effect of FA and RM on P mobilisation using plant growth experiment. Among the amendments, FA showed the highest increase in Olsen P, oxalic acid content and phosphatase activity. The highest increase in Olsen P for PM treated non-irrigated soils showed the ability of FA and RM in mobilising organic P better than inorganic P (PP). There was over 85 % increase in oxalic acid content in the plant growth soils compared to the incubated soil, showing the effect of Napier grass in the exudation of oxalic acid. Both amendments (FA and RM) showed an increase in phosphatase activity at over 90 % at the end of the 5-week incubation period. The leaching experiment indicated a decrease in water soluble P thereby ensuring the role of FA and RM in minimising P loss to water bodies. FA and RM showed an increase in plant biomass for all treatments, where FA amended soil showed the highest increase as evident from FA's effect on Olsen P. Therefore, the use of FA and RM mobilised P in abattoir wastewater irrigated soils and increased biomass production of Napier grass plants through root exudation of oxalic acid. PMID:24862480

  5. Hydrogen production from biomass gasification using biochar as a catalyst/support.

    PubMed

    Yao, Dingding; Hu, Qiang; Wang, Daqian; Yang, Haiping; Wu, Chunfei; Wang, Xianhua; Chen, Hanping

    2016-09-01

    Biochar is a promising catalyst/support for biomass gasification. Hydrogen production from biomass steam gasification with biochar or Ni-based biochar has been investigated using a two stage fixed bed reactor. Commercial activated carbon was also studied as a comparison. Catalyst was prepared with an impregnation method and characterized by X-ray diffraction, specific surface and porosity analysis, X-ray fluorescence and scanning electron micrograph. The effects of gasification temperature, steam to biomass ratio, Ni loading and bio-char properties on catalyst activity in terms of hydrogen production were explored. The Ni/AC catalyst showed the best performance at gasification temperature of 800°C, S/B=4, Ni loading of 15wt.%. Texture and composition characterization of the catalysts suggested the interaction between volatiles and biochar promoted the reforming of pyrolysis volatiles. Cotton-char supported Ni exhibited the highest activity of H2 production (64.02vol.%, 92.08mgg(-1) biomass) from biomass gasification, while rice-char showed the lowest H2 production. PMID:27240230

  6. Biomass, lipid productivities and fatty acids composition of marine Nannochloropsis gaditana cultured in desalination concentrate.

    PubMed

    Matos, Ângelo Paggi; Feller, Rafael; Moecke, Elisa Helena Siegel; Sant'Anna, Ernani Sebastião

    2015-12-01

    In this study the feasibility of growing marine Nannochloropsis gaditana in desalination concentrate (DC) was explored and the influence of the DC concentration on the biomass growth, lipid productivities and fatty acids composition was assessed. The reuse of the medium with the optimum DC concentration in successive algal cultivation cycles and the additional of a carbon source to the optimized medium were also evaluated. On varying the DC concentration, the maximum biomass concentration (0.96gL(-1)) and lipid content (12.6%) were obtained for N. gaditana in the medium with the optimum DC concentration (75%). Over the course of the reuse of the optimum DC medium, three cultivation cycles were performed, observing that the biomass productivity is directly correlated to lipid productivity. Palmitic acid was the major fatty acid found in N. gaditana cells. The saturated fatty acids content of the algae enhanced significantly on increasing the DC concentration. PMID:26318921

  7. Optimization of cellulase production by Enhydrobacter sp. ACCA2 and its application in biomass saccharification

    PubMed Central

    Premalatha, Nagaiah; Gopal, Nellaiappan O.; Jose, Polpass Arul; Anandham, Rangasamy; Kwon, Soon-Wo

    2015-01-01

    Cellulase finds use in saccharification of lignocellulosic agroresidues to fermentable sugars which can be used for production of commercially important metabolites. This study reports endoglucanase (CMCase) production by Enhydrobacter sp. ACCA2. The CMCase activity of the strain ACCA2 was successively improved by optimization of range of physical and nutritional parameter in a set of non-statistical and statistical experiments. Initial non-statistical selection of carbon source, incubation time, temperature and pH resulted in 1.07 fold increase of CMCase activity. In a subsequent statistical method, response surface methodology, optimization of medium components such as carboxymethylcellulose, peptone, NaCl, MgSO4, K2HPO4, and (NH4)2SO4 yielded further increase up to 2.39 fold CMCase activity. The cellulolytic potential was evaluated in biomass saccharification with different plant materials and the results revealed that the enzyme produced by strain may have significant commercial values for industrial saccharification process. Moreover, this is the first report of cellulase production by an Enhydrobacter spp. PMID:26500615

  8. Polypropylene Bundle Attached Multilayered Stigeoclonium Biofilms Cultivated in Untreated Sewage Generate High Biomass and Lipid Productivity.

    PubMed

    Kim, Byung-Hyuk; Kim, Dong-Ho; Choi, Jung-Woon; Kang, Zion; Cho, Dae-Hyun; Kim, Ji-Young; Oh, Hee-Mock; Kim, Hee-Sik

    2015-09-01

    The potential of microalgae biofuel has not been realized because of the low productivity and high costs associated with the current cultivation systems. In this study, a new low-cost and transparent attachment material was tested for cultivation of a filamentous algal strain, Stigeoclonium sp., isolated from wastewater. Initially, the different materials tested for Stigeoclonium cultivation in untreated wastewater were nylon mesh, polyethylene mesh, polypropylene bundle (PB), polycarbonate plate, and viscose rayon. Among the materials tested, PB led to a firm attachment, high biomass (53.22 g/m(2), dry cell weight), and total lipid yield (5.8 g/m(2)) with no perceivable change in FAME profile. The Stigeoclonium-dominated biofilm consisted of bacteria and extracellular polysaccharide, which helped in biofilm formation and for effective wastewater treatment (viz., removal efficiency of total nitrogen and total phosphorus corresponded to ~38% and ~90%, respectively). PB also demonstrated high yields under multilayered cultivation in a single reactor treating wastewater. Hence, this system has several advantages over traditional suspended and attached systems, with possibility of increasing areal productivity three times using Stigeoclonium sp. Therefore, multilayered attached growth algal cultivation systems seem to be the future cultivation model for large-scale biodiesel production and wastewater treatment. PMID:25951844

  9. An analysis of alternative technologies for the removal of ethylene from the CELSS biomass production chamber

    NASA Technical Reports Server (NTRS)

    Rakow, Allen L.

    1995-01-01

    A variety of technologies were analyzed for their potential to remove ethylene from the CELSS Biomass Production Chamber (BPC). During crop production (e.g., lettuce, wheat, soybean, potato) in the BPC ethylene can accumulate in the airspace and subsequently affect plant viability. The chief source of ethylene is the plants themselves which reside in plastic trays containing nutrient solution. The main sink for ethylene is chamber leakage. The removal technology can be employed when deleterious levels (e.g., 50 ppb for potato) of ethylene are exceeded in the BPC and perhaps to optimize the plant growth process once a better understanding is developed of the relationship between exogenous ethylene concentration and plant growth. The technologies examined were catalytic oxidation, molecular sieve, cryotrapping, permanganate absorption, and UV degradation. Upon analysis, permanganate was chosen as the most suitable method. Experimental data for ethylene removal by permanganate during potato production was analyzed in order to design a system for installation in the BPC air duct. In addition, an analysis of the impact on ethylene concentration in the BPC of integrating the Breadboard Scale Aerobic Bioreactor (BSAB) with the BPC was performed. The result indicates that this unit has no significant effect on the ethylene material balance as a source or sink.

  10. Resource Assessment for Hydrogen Production: Hydrogen Production Potential from Fossil and Renewable Energy Resources

    SciTech Connect

    Melaina, M.; Penev, M.; Heimiller, D.

    2013-09-01

    This study examines the energy resources required to produce 4-10 million metric tonnes of domestic, low-carbon hydrogen in order to fuel approximately 20-50 million fuel cell electric vehicles. These projected energy resource requirements are compared to current consumption levels, projected 2040 business as usual consumptions levels, and projected 2040 consumption levels within a carbonconstrained future for the following energy resources: coal (assuming carbon capture and storage), natural gas, nuclear (uranium), biomass, wind (on- and offshore), and solar (photovoltaics and concentrating solar power). The analysis framework builds upon previous analysis results estimating hydrogen production potentials and drawing comparisons with economy-wide resource production projections

  11. RENEWABLE ENERGY AND ENVIRONMENTAL SUSTAINABILITY USING BIOMASS FROM DAIRY AND BEEF ANIMAL PRODUCTION

    SciTech Connect

    Kalyan Annamalai, John M. Sweeten, Brent W. Auvermann, Saqib Mukhtar, Sergio Caperada Cady R. Engler, Wyatte Harman Reddy JN Robert Deotte

    2012-05-03

    The Texas Panhandle is regarded as the 'Cattle Feeding Capital of the World', producing 42% of the fed beef cattle in the United States within a 200-mile radius of Amarillo generating more than 5 million tons of feedlot manure/year. Apart from feedlots, the Bosque River Region in Erath County, just north of Waco, Texas with about 110,000 dairy cattle in over 250 dairies, produces 1.8 million tons of manure biomass (excreted plus bedding) per year. While the feedlot manure has been used extensively for irrigated and dry land crop production, most dairies, as well as other concentrated animal feeding operations (CAFO's), the dairy farms utilize large lagoon areas to store wet animal biomass. Water runoff from these lagoons has been held responsible for the increased concentration of phosphorus and other contaminates in the Bosque River which drains into Lake Waco - the primary source of potable water for Waco's 108,500 people. The concentrated animal feeding operations may lead to land, water, and air pollution if waste handling systems and storage and treatment structures are not properly managed. Manure-based biomass (MBB) has the potential to be a source of green energy at large coal-fired power plants and on smaller-scale combustion systems at or near confined animal feeding operations. Although MBB particularly cattle biomass (CB) is a low quality fuel with an inferior heat value compared to coal and other fossil fuels, the concentration of it at large animal feeding operations can make it a viable source of fuel. The overall objective of this interdisciplinary proposal is to develop environmentally benign technologies to convert low-value inventories of dairy and beef cattle biomass into renewable energy. Current research expands the suite of technologies by which cattle biomass (CB: manure, and premature mortalities) could serve as a renewable alternative to fossil fuel. The work falls into two broad categories of research and development. Category 1

  12. Synergies between bio- and oil refineries for the production of fuels from biomass.

    PubMed

    Huber, George W; Corma, Avelino

    2007-01-01

    As petroleum prices continue to increase, it is likely that biofuels will play an ever-increasing role in our energy future. The processing of biomass-derived feedstocks (including cellulosic, starch- and sugar-derived biomass, and vegetable fats) by catalytic cracking and hydrotreating is a promising alternative for the future to produce biofuels, and the existing infrastructure of petroleum refineries is well-suited for the production of biofuels, allowing us to rapidly transition to a more sustainable economy without large capital investments for new reaction equipment. This Review discusses the chemistry, catalysts, and challenges involved in the production of biofuels. PMID:17610226

  13. Economic feasibility of agricultural alcohol production within a biomass system

    SciTech Connect

    Hertzmark, D.; Flaim, S.; Ray, D.; Parvin, G.

    1980-12-01

    The technical and economic feasibility of agricultural alcohol production in the United States is discussed. The beverage fermentation processes are compared and contrasted with the wet milling of corn, and alternative agricultural products for alcohol production are discussed. Alcohol costs for different fermentation methods and for various agricultural crops (corn, sugar cane, sugar beets, etc.) are presented, along with a brief discussion of US government policy implications. (JMT)

  14. Fuel production potential of several agricultural crops

    SciTech Connect

    Mays, D.A.; Buchanan, W.; Bradford, B.N.

    1984-11-01

    Data collected on starch and sugar crops indicate that sweet potato and sweet sorghum have the best potential for alcohol production in the TVA area. Of the oil crops evaluated in this series of experiments only sunflower and okara appear to offer potential in the Tennessee Valley for oil production for fuel or other uses. 21 tabs.

  15. The flash pyrolysis and methanolysis of biomass (wood) for production of ethylene, benzene and methanol

    SciTech Connect

    Steinberg, M.; Fallon, P.T.; Sundaram, M.S.

    1990-02-01

    The process chemistry of the flash pyrolysis of biomass (wood) with the reactive gases, H{sub 2} and CH{sub 4} and with the non-reactive gases He and N{sub 2} is being determined in a 1 in. downflow tubular reactor at pressures from 20 to 1000 psi and temperatures from 600 to 1000{degrees}C. With hydrogen, flash hydropyrolysis leads to high yields of methane and CO which can be used for SNG and methanol fuel production. With methane, flash methanolysis leads to high yields of ethylene, benzene and CO which can be used for the production of valuable chemical feedstocks and methanol transportation fuel. At reactor conditions of 50 psi and 1000{degrees}C and approximately 1 sec residence time, the yields based on pine wood carbon conversion are up to 25% for ethylene, 25% for benzene, and 45% for CO, indicating that over 90% of the carbon in pine is converted to valuable products. Pine wood produces higher yields of hydrocarbon products than Douglas fir wood; the yield of ethylene is 2.3 times higher with methane than with helium or nitrogen, and for pine, the ratio is 7.5 times higher. The mechanism appears to be a free radical reaction between CH{sub 4} and the pyrolyzed wood. There appears to be no net production or consumption of methane. A preliminary process design and analysis indicates a potentially economical competitive system for the production of ethylene, benzene and methanol based on the methanolysis of wood. 10 refs., 18 figs., 1 tab.

  16. Effects of Nitrogen and Desferal Treatments on CROTALARIA's (Crotalaria juncea Roth) Biomass Production

    NASA Astrophysics Data System (ADS)

    László Phd, M., ,, Dr.

    2009-04-01

    , depending on the N+Desferal treatment rates. g. On the given soil the highest quantity of 300 kg ha-1 year-1 N mineral fertilizer + 20 kg ha-1 year-1 Desferal chelating agent seemed to give already over fertilization and lowered in its tendency mainly the total green biomass yield and verifiable the total air dry biomass yield. By experimental results can be summarised that we was able to increase with a great rate Crotalaria juncea L. total green biomass yield and total air dry biomass yield by N and Desferal treatments. As well as so we can show and offer our results to different sustainable farming managements with nutrition system of low, mean and high input in the next future. Keywords: nitrogen, desferal, crotalaria, soil fertility, sustainable agriculture management Introduction Presently sustainable agriculture is vital to achieving food security poverty alleviation and environmental protection because land degradation and desertification has occurred in all the world over cutting across a broad spectrum of contrasts in climate, ecosystem types, land uses and socio/economic settings. For this reason improving integrated soil fertility management is appreciationed and has become a major issue of concern on the development plant nutrition and plant production agendas. On plant nutrition level mineral macronutrients so nitrogen and chelating agents of different microelements so Desferal- deferoxamin-methansulfonic are essential for plant growth and development. Crotalari juncea L. is a well-known nutrient indicator fodder and green manure crop with a high yield potential. The complexity of the desertification phenomenon has drawn our attention to categorize, inventory, monitor and repair the condition of the land (Arnalds and Archer 1999). Therefore, farmers are constantly being subjected to soil fertility changes that are beyond their control on account of factors that affect the viability and profitability their farming enterprise. They must acquire the capacity to

  17. Fed-batch Fermentation of Lactic Acid Bacteria to Improve Biomass Production: A Theoretical Approach

    NASA Astrophysics Data System (ADS)

    Beng Lee, Boon; Tham, Heng Jin; Chan, Eng Seng

    Recently, fed-batch fermentation has been introduced in an increasing number of fermentation processes. Previous researches showed that fed-batch fermentation can increase the biomass yield of many strains. Improvement of the biomass yield is interested because biomass from lactic acid bacteria (LAB) fermentation is widely used in food and pharmaceutical industry. The aim of this research is to study the ability and feasibility of fed-batch fermentation to improve biomass production of LAB. Appropriate model has been selected from literature. Monod equation described the substrate limitation of LAB and the product inhibition of LAB follows a non-competitive model. Furthermore, the lactic acid production follows Luedeking and Piret model. Then the models are applied to simulate the fermentation of batch and fed-batch cultures by using MATLAB. From the results of simulation, fed-batch fermentation showed that substrate limitation and substrate inhibition can be avoided. Besides that, the variable volume fed-batch fermentation also showed that product inhibition can be eliminated by diluting the product concentration with added fresh feed. However, it was found that fed-batch fermentation is not economically feasible because large amount of substrate is required to reduce the product inhibition effect. Therefore, fed-batch fermentation plays more importance role if the fermentation strain has high Ks value or low Kp value.

  18. Geographic and Habitat Origin Influence Biomass Production and Storage Translocation in the Clonal Plant Aegopodium podagraria

    PubMed Central

    D′Hertefeldt, Tina; Eneström, Johanna M.; Pettersson, Lars B.

    2014-01-01

    Through physiological integration, clonal plants can support ramets in unfavourable patches, exploit heterogeneously distributed resources and distribute resources that are taken up over large areas. Physiological integration generally increases in adverse conditions, but it is not well known which factors determine the evolution of physiological integration. The aim of this study was to investigate if clonal plants from Southern and Northern populations of the clonal herb Aegopodium podagraria differed in physiological integration in terms of translocation of carbon to the rhizomes, and in biomass production using a reciprocal transplant experiment. Aegopodium podagraria from shaded conditions have been suggested to share more resources than clones from open conditions and therefore, plants from forest and open populations within the Southern and Northern regions were included. The regional growing conditions greatly affected biomass production. Plants grown in North Sweden produced more biomass and allocated more biomass to shoots, while plants grown in South Sweden allocated more biomass to rhizomes. There was a regional origin effect as plants originating from North Sweden produced more biomass in both regions. Within the Northern region, plants from shaded habitats translocated more 14C to the rhizomes, suggesting more storage there than in plants from open habitats. In addition to genetic differentiation in biomass production between Northern and Southern populations, probably as a response to a shorter growing season in the North, there appeared to be genetic differentiation in physiological integration within the Northern region. This shows that both regional and local conditions need to be taken into account in future studies of genetic differentiation of physiological integration in clonal plants. PMID:24427305

  19. Effects of species diversity on community biomass production change over the course of succession.

    PubMed

    Weis, Jerome J; Cardinale, Bradley J; Forshay, Kenneth J; Ives, Anthony R

    2007-04-01

    Over the past decade an increasing number of studies have experimentally manipulated the number of species in a community and examined how this alters the aggregate production of species biomass. Many of these studies have shown that the effects of richness on biomass change through time, but we have limited understanding of the mechanisms that produce these dynamic trends. Here we report the results of an experiment in which we manipulated the richness of freshwater algae in laboratory microcosms. We used two experimental designs (additive and substitutive) that make different assumptions about how patches are initially colonized, and then tracked the development of community biomass from the point of initial colonization through a period of 6-12 generations of the focal species. We found that the effect of initial species richness on biomass production qualitatively shifted twice over the course of the experiment. The first shift occurred as species transitioned from density-independent to dependent phases of population growth. At this time, intraspecific competition caused monocultures to approach their respective carrying capacities more slowly than polycultures. As a consequence, species tended to over-yield for a brief time, generating a positive, but transient effect of diversity on community biomass. The second shift occurred as communities approached carrying capacity. At this time, strong interspecific interactions caused biomass to be dominated by the competitively superior species in polycultures. As this species had the lowest carrying capacity, a negative effect of diversity on biomass resulted in late succession. Although these two shifts produced dynamics that appeared complex, we show that the patterns can be fit to a simple Lotka-Volterra model of competition. Our results suggest that the effects of algal diversity on primary production change in a predictable sequence through successional time. PMID:17536709

  20. A Review on Biomass Torrefaction Process and Product Properties for Energy Applications

    SciTech Connect

    Jaya Shankar Tumuluru; Shahab Sokhansanj; J. Richard Hess; Christopher T. Wright; Richard D. Boardman

    2011-10-01

    Torrefaction of biomass can be described as a mild form of pyrolysis at temperatures typically ranging between 200 and 300 C in an inert and reduced environment. Common biomass reactions during torrefaction include devolatilization, depolymerization, and carbonization of hemicellulose, lignin and cellulose. Torrefaction process produces a brown to black solid uniform product and also condensable (water, organics, and lipids) and non condensable gases (CO2, CO, and CH4). Typically during torrefaction, 70% of the mass is retained as a solid product, containing 90% of the initial energy content, and 30% of the lost mass is converted into condensable and non-condensable products. The system's energy efficiency can be improved by reintroducing the material lost during torrefaction as a source of heat. Torrefaction of biomass improves its physical properties like grindability; particle shape, size, and distribution; pelletability; and proximate and ultimate composition like moisture, carbon and hydrogen content, and calorific value. Carbon and calorific value of torrefied biomass increases by 15-25%, and moisture content reduces to <3% (w.b.). Torrefaction reduces grinding energy by about 70%, and the ground torrefied biomass has improved sphericity, particle surface area, and particle size distribution. Pelletization of torrefied biomass at temperatures of 225 C reduces specific energy consumption by two times and increases the capacity of the mill by two times. The loss of the OH group during torrefaction makes the material hydrophobic (loses the ability to attract water molecules) and more stable against chemical oxidation and microbial degradation. These improved properties make torrefied biomass particularly suitable for cofiring in power plants and as an upgraded feedstock for gasification.

  1. Oligosaccharides and monomeric carbohydrates production from olive tree pruning biomass.

    PubMed

    Mateo, Soledad; Puentes, Juan G; Sánchez, Sebastián; Moya, Alberto J

    2013-04-01

    Using the severity factor, it has been possible to study cellulose and hemicellulose fractional conversion, sugar yields change and oligosaccharides variation through olive tree pruning biomass pretreatments with acid or liquid hot water under pressure. The temperatures tested were in the range 180-230°C, operation time varying between 0 and 30min and acid concentration used did not exceed 0.05M. Complete hemicellulose solubilization in autohydrolysis was achieved using severity factors (logR0) close to 3.9 (most sugars are like oligomers), while if sulfuric acid 0.025M is employed, this parameter could be smaller (≥3.4). With these treatments, we have obtained cellulose conversions between 30 and 42% from liquid hot water experiments, 40-51% with sulfuric acid 0.025M and 42-57% when the acid concentration was 0.05M. The best results in terms of maximum yield in total sugars, d-glucose and d-xylose, with a low amount of acetic acid and hydroxymethylfurfural, was obtained at 200°C, 0min (what means that there is no time of temperature maintenance, only heating and cooling) and H2SO4 0.025M. PMID:23499077

  2. Biomass production from sugarcane and sweet sorghum. Final report

    SciTech Connect

    Gascho, G.J.; Shih, S.F.

    1980-01-01

    The results of a field study on growing sugarcane and sweet sorghum in the Lake Okeechobee area of Florida are reported. Two experiments were conducted on row-spacing of sugarcane and one on row-spacing of sorghum. There were no surprises in the data obtained in this year's sugarcane experiments. High biomass, sugar and fiber were produced both on sand and muck soils in south Florida. Yields were, as in previous years, higher for the narrow row spacing where solar radiation was better than in plant cane. Likewise it is greater for a second ratoon than for a first ratoon. Sweet sorghum produced well but not as well as last year due to a planting data which was 1 to 2 months late and to the wider spacings used to facilitate the trial of sugarcane harvesting equipment. Moisture is much more critical for sorghum than for cane. One experiment on muck suffered due to wet conditions. A second experiment on sand was lost due to lack of moisture.

  3. Hydrogen production from algal biomass via steam gasification.

    PubMed

    Duman, Gozde; Uddin, Md Azhar; Yanik, Jale

    2014-08-01

    Algal biomasses were tested as feedstock for steam gasification in a dual-bed microreactor in a two-stage process. Gasification experiments were carried out in absence and presence of catalyst. The catalysts used were 10% Fe₂O₃-90% CeO₂ and red mud (activated and natural forms). Effects of catalysts on tar formation and gasification efficiencies were comparatively investigated. It was observed that the characteristic of algae gasification was dependent on its components and the catalysts used. The main role of the catalyst was reforming of the tar derived from algae pyrolysis, besides enhancing water gas shift reaction. The tar reduction levels were in the range of 80-100% for seaweeds and of 53-70% for microalgae. Fe₂O₃-CeO₂ was found to be the most effective catalyst. The maximum hydrogen yields obtained were 1036 cc/g algae for Fucus serratus, 937 cc/g algae for Laminaria digitata and 413 cc/g algae for Nannochloropsis oculata. PMID:24880809

  4. Lipid Production of Heterotrophic Chlorella sp. from Hydrolysate Mixtures of Lipid-Extracted Microalgal Biomass Residues and Molasses.

    PubMed

    Zheng, Hongli; Ma, Xiaochen; Gao, Zhen; Wan, Yiqin; Min, Min; Zhou, Wenguang; Li, Yun; Liu, Yuhuan; Huang, He; Chen, Paul; Ruan, Roger

    2015-10-01

    This study investigated the feasibility of lipid production of Chlorella sp. from waste materials. Lipid-extracted microalgal biomass residues (LMBRs) and molasses were hydrolyzed, and their hydrolysates were analyzed. Five different hydrolysate mixture ratios (w/w) of LMBRs/molasses (1/0, 1/1, 1/4, 1/9, and 0/1) were used to cultivate Chlorella sp. The results showed that carbohydrate and protein were the two main compounds in the LMBRs, and carbohydrate was the main compound in the molasses. The highest biomass concentration of 5.58 g/L, Y biomass/sugars of 0.59 g/g, lipid productivity of 335 mg/L/day, and Y lipids/sugars of 0.25 g/g were obtained at the hydrolysate mixture ratio of LMBRs/molasses of 1/4. High C/N ratio promoted the conversion of sugars into lipids. The lipids extracted from Chlorella sp. shared similar lipid profile of soybean oil and is therefore a potential viable biodiesel feedstock. These results showed that Chlorella sp. can utilize mixed sugars and amino acids from LMBRs and molasses to accumulate lipids efficiently, thus reducing the cost of microalgal biodiesel production and improving its economic viability. PMID:26234438

  5. Managing water resources for biomass production in a biofuel economy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    One goal of our national security policy is to become more energy independent using biofuels. The expanded production of agricultural crops for bioenergy production has introduced new challenges for management of water. Water availability has been widely presumed in the discussion of bioenergy crop ...

  6. Biotechnology of biomass conversion

    SciTech Connect

    Wayman, M.; Parekh, S.R.

    1990-01-01

    This book covers: An introduction to biomass crops; The microbiology of fermentation processes; The production of ethanol from biomass crops, such as sugar cane and rubbers; The energy of biomass conversion; and The economics of biomass conversion.

  7. Atmospheric leakage and condensate production in NASA's biomass production chamber. Effect of diurnal temperature cycles

    NASA Technical Reports Server (NTRS)

    Wheeler, Raymond M.; Drese, John H.; Sager, John C.

    1991-01-01

    A series of tests were conducted to monitor atmospheric leakage rate and condensate production in NASA's Biomass Production Chamber (BPC). Water was circulated through the 64 plant culture trays inside the chamber during the tests but no plants were present. Environmental conditions were set to a 12-hr photoperiod with either a matching 26 C (light)/20 C (dark) thermoperiod, or a constant 23 C temperature. Leakage, as determined by carbon dioxide decay rates, averaged about 9.8 percent for the 26 C/20 C regime and 7.3 percent for the constant 23 C regime. Increasing the temperature from 20 C to 26 C caused a temporary increase in pressure (up to 0.5 kPa) relative to ambient, while decreasing the temperature caused a temporary decrease in pressure of similar magnitude. Little pressure change was observed during transition between 23 C (light) and 23 C (dark). The lack of large pressure events under isothermal conditions may explain the lower leakage rate observed. When only the plant support inserts were placed in the culture trays, condensate production averaged about 37 liters per day. Placing acrylic germination covers over the tops of culture trays reduced condensate production to about 7 liters per day. During both tests, condensate production from the lower air handling system was 60 to 70 percent greater than from the upper system, suggesting imbalances exist in chilled and hot water flows for the two air handling systems. Results indicate that atmospheric leakage rates are sufficiently low to measure CO2 exchange rates by plants and the accumulation of certain volatile contaminants (e.g., ethylene). Control system changes are recommended in order to balance operational differences (e.g., humidity and temperature) between the two halves of the chamber.

  8. 2007 Biomass Program Overview

    SciTech Connect

    none,

    2009-10-27

    The Biomass Program is actively working with public and private partners to meet production and technology needs. With the corn ethanol market growing steadily, researchers are unlocking the potential of non-food biomass sources, such as switchgrass and forest and agricultural residues. In this way, the Program is helping to ensure that cost-effective technologies will be ready to support production goals for advanced biofuels.

  9. Artificial defoliation effect on Populus growth, biomass production, and total nonstructural carbohydrate concentration

    SciTech Connect

    Reichenbacker, R.R.; Hart, E.R.; Schultz, R.C.

    1996-06-01

    The impact of artificial defoliation on Populus growth, biomass production, and total nonstructural carbohydrate concentration was examined. Four Populus clones were field planted and artificially defoliated. Assigned defoliation levels (0, 25, 50, or 75%) were applied to leaves of leaf plastochron index 0 through 8 during a 6-d period in a 3-step incremental manner to simulate cottonwood leaf beetle, Chrysomela scripta F., larval feeding patterns. Artificial defoliations were timed to coincide with the outbreaks of natural beetle populations in adjacent areas. After 2 growing seasons, trees were measured for height, diameter, and biomass accumulation. Root samples were collected from 0 and 75% defoliation treatments for each clone. Biomass was reduced an average of 33% as defoliation level increased from 0 to 75%. As defoliation level increased from 0 to 75%, a consistent allocation ratio of biomass to 2/3 above and 1/3 below ground components continued in all clones. An overcompensation response occurred in above ground biomass when a defoliation level of 25% was applied. Between 25 and 75% a strong linear trend of decreasing biomass as defoliation increased was indicated. Vitality of the tree, as indicated by total nonstructural carbohydrate content, was affected only slightly by increasing defoliation. 26 refs., 1 fig., 6 tabs.

  10. Maximum hydrogen production from genetically modified microalgae biomass

    NASA Astrophysics Data System (ADS)

    Vargas, Jose; Kava, Vanessa; Ordonez, Juan

    A transient mathematical model for managing microalgae derived H2 production as a source of renewable energy is developed for a well stirred photobioreactor, PBR. The model allows for the determination of microalgae and H2 mass fractions produced by the PBR in time. A Michaelis-Menten expression is proposed for modeling the rate of H2 production, which introduces an expression to calculate the resulting effect on H2 production rate after genetically modifying the microalgae. The indirect biophotolysis process was used. Therefore, an opportunity was found to optimize the aerobic to anaerobic stages time ratio of the cycle for maximum H2 production rate, i.e., the process rhythm. A system thermodynamic optimization is conducted with the model equations to find accurately the optimal system operating rhythm for maximum H2 production rate, and how wild and genetically modified species compare to each other. The maxima found are sharp, showing up to a ~60% variation in hydrogen production rate within 2 days around the optimal rhythm, which highlights the importance of system operation in such condition. Therefore, the model is expected to be useful for design, control and optimization of H2 production. Brazilian National Council of Scientific and Technological Development, CNPq (project 482336/2012-9).

  11. Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review

    DOE PAGESBeta

    Dong, Tao; Knoshaug, Eric P.; Pienkos, Philip T.; Laurens, Lieve M. L.

    2016-06-15

    Biological lipids derived from oleaginous microorganisms are promising precursors for renewable biofuel productions. Direct lipid extraction from wet cell-biomass is favored because it eliminates the need for costly dehydration. However, the development of a practical and scalable process for extracting lipids from wet cell-biomass is far from ready to be commercialized, instead, requiring intensive research and development to understand the lipid accessibility, mechanisms in mass transfer and establish robust lipid extraction approaches that are practical for industrial applications. Furthermore, this paper aims to present a critical review on lipid recovery in the context of biofuel productions with special attention tomore » cell disruption and lipid mass transfer to support extraction from wet biomass.« less

  12. Comparative study of thermochemical processes for hydrogen production from biomass fuels.

    PubMed

    Biagini, Enrico; Masoni, Lorenzo; Tognotti, Leonardo

    2010-08-01

    Different thermochemical configurations (gasification, combustion, electrolysis and syngas separation) are studied for producing hydrogen from biomass fuels. The aim is to provide data for the production unit and the following optimization of the "hydrogen chain" (from energy source selection to hydrogen utilization) in the frame of the Italian project "Filiera Idrogeno". The project focuses on a regional scale (Tuscany, Italy), renewable energies and automotive hydrogen. Decentred and small production plants are required to solve the logistic problems of biomass supply and meet the limited hydrogen infrastructures. Different options (gasification with air, oxygen or steam/oxygen mixtures, combustion, electrolysis) and conditions (varying the ratios of biomass and gas input) are studied by developing process models with uniform hypothesis to compare the results. Results obtained in this work concern the operating parameters, process efficiencies, material and energetic needs and are fundamental to optimize the entire hydrogen chain. PMID:20362431

  13. The influence of light intensity and photoperiod on duckweed biomass and starch accumulation for bioethanol production.

    PubMed

    Yin, Yehu; Yu, Changjiang; Yu, Li; Zhao, Jinshan; Sun, Changjiang; Ma, Yubin; Zhou, Gongke

    2015-01-01

    Duckweed has been considered as a valuable feedstock for bioethanol production due to its high biomass and starch production. To investigate the effects of light conditions on duckweed biomass and starch production, Lemna aequinoctialis 6000 was cultivated at different photoperiods (12:12, 16:8 and 24:0h) and light intensities (20, 50, 80, 110, 200 and 400μmolm(-2)s(-1)). The results showed that the duckweed biomass and starch production was increased with increasing light intensity and photoperiod except at 200 and 400μmolm(-2)s(-1). Considering the light cost, 110μmolm(-2)s(-1) was optimum light condition for starch accumulation with the highest maximum growth rate, biomass and starch production of 8.90gm(-2)day(-1), 233.25gm(-2) and 98.70gm(-2), respectively. Moreover, the results suggested that high light induction was a promising method for duckweed starch accumulation. This study provides optimized light conditions for future industrial large-scale duckweed cultivation. PMID:25841186

  14. Co-fermentation of acetate and sugars facilitating microbial lipid production on acetate-rich biomass hydrolysates.

    PubMed

    Gong, Zhiwei; Zhou, Wenting; Shen, Hongwei; Yang, Zhonghua; Wang, Guanghui; Zuo, Zhenyu; Hou, Yali; Zhao, Zongbao K

    2016-05-01

    The process of lignocellulosic biomass routinely produces a stream that contains sugars plus various amounts of acetic acid. As acetate is known to inhibit the culture of microorganisms including oleaginous yeasts, little attention has been paid to explore lipid production on mixtures of acetate and sugars. Here we demonstrated that the yeast Cryptococcus curvatus can effectively co-ferment acetate and sugars for lipid production. When mixtures of acetate and glucose were applied, C. curvatus consumed both substrates simultaneously. Similar phenomena were also observed for acetate and xylose mixtures, as well as acetate-rich corn stover hydrolysates. More interestingly, the replacement of sugar with equal amount of acetate as carbon source afforded higher lipid titre and lipid content. The lipid products had fatty acid compositional profiles similar to those of cocoa butter, suggesting their potential for high value-added fats and biodiesel production. This co-fermentation strategy should facilitate lipid production technology from lignocelluloses. PMID:26874438

  15. Exploring the Utilization of Complex Algal Communities to Address Algal Pond Crash and Increase Annual Biomass Production for Algal Biofuels

    SciTech Connect

    Hamilton, Cyd E.

    2014-03-25

    This white paper briefly reviews the research literature exploring complex algal communities as a means of increasing algal biomass production via increased tolerance, resilience, and resistance to a variety of abiotic and biotic perturbations occurring within harvesting timescales. This paper identifies what data are available and whether more research utilizing complex communities is needed to explore the potential of complex algal community stability (CACS) approach as a plausible means to increase biomass yields regardless of ecological context and resulting in decreased algal-based fuel prices by reducing operations costs. By reviewing the literature for what we do and do not know, in terms of CACS methodologies, this report will provide guidance for future research addressing pond crash phenomena.

  16. Production of mycelial biomass by the Amazonian edible mushroom Pleurotus albidus.

    PubMed

    Kirsch, Larissa de Souza; de Macedo, Ana Júlia Porto; Teixeira, Maria Francisca Simas

    2016-01-01

    Edible mushroom species are considered as an adequate source of food in a healthy diet due to high content of protein, fiber, vitamins, and a variety of minerals. The representatives of Pleurotus genus are characterized by distinct gastronomic, nutritional, and medicinal properties among the edible mushrooms commercialized worldwide. In the present study, the growth of mycelial biomass of Pleurotus albidus cultivated in submerged fermentation was evaluated. Saccharose, fructose, and maltose were the three main carbon sources for mycelial biomass formation with corresponding yields of 7.28gL(-1), 7.07gL(-1), and 6.99gL(-1). Inorganic nitrogen sources did not stimulate growth and the optimal yield was significantly higher with yeast extract (7.98gL(-1)). The factorial design used to evaluate the influence of saccharose and yeast extract concentration, agitation speed, and initial pH indicated that all variables significantly influenced the production of biomass, especially the concentration of saccharose. The greater amount of saccharose resulted in the production of significantly more biomass. The highest mycelial biomass production (9.81gL(-1)) was reached in the medium formulated with 30.0gL(-1) saccharose, 2.5gL(-1) yeast extract, pH 7.0, and a speed of agitation at 180rpm. Furthermore, P. albidus manifested different aspects of morphology and physiology under the growth conditions employed. Media composition affected mycelial biomass production indicating that the diversification of carbon sources promoted its improvement and can be used as food or supplement. PMID:27266626

  17. Interactions between herbivory and warming in aboveground biomass production of arctic vegetation

    PubMed Central

    Pedersen, Christian; Post, Eric

    2008-01-01

    Background Many studies investigating the ecosystem effects of global climate change have focused on arctic ecosystems because the Arctic is expected to undergo the earliest and most pronounced changes in response to increasing global temperatures, and arctic ecosystems are considerably limited by low temperatures and permafrost. In these nutrient limited systems, a warmer climate is expected to increase plant biomass production, primarily through increases in shrubs over graminoids and forbs. But, the influence of vertebrate and invertebrate herbivores has been largely absent in studies investigating the effects of vegetation responses to climate change, despite the fact that herbivory can have a major influence on plant community composition, biomass and nutrient cycling. Here, we present results from a multi-annual field experiment investigating the effects of vertebrate herbivory on plant biomass response to simulated climate warming in arctic Greenland. Results The results after four years of treatments did not give any clear evidence of increased biomass of shrubs in response climate warming. Nor did our study indicate that vertebrate grazing mediated any increased domination of shrubs over other functional plant groups in response to warming. However, our results indicate an important role of insect outbreaks on aboveground biomass. Intense caterpillar foraging from a two-year outbreak of the moth Eurois occulta during two growing seasons may have concealed any treatment effects. However, there was some evidence suggesting that vertebrate herbivores constrain the biomass production of shrubs over graminoids and forbs. Conclusion Although inconclusive, our results were likely constrained by the overwhelming influence of an unexpected caterpillar outbreak on aboveground biomass. It is likely that the role of large vertebrate herbivores in vegetation response to warming will become more evident as this experiment proceeds and the plant community recovers from

  18. %22Trojan Horse%22 strategy for deconstruction of biomass for biofuels production.

    SciTech Connect

    Simmons, Blake Alexander; Sinclair, Michael B.; Yu, Eizadora; Timlin, Jerilyn Ann; Hadi, Masood Z.; Tran-Gyamfi, Mary

    2011-02-01

    Production of renewable biofuels to displace fossil fuels currently consumed in the transportation sector is a pressing multiagency national priority (DOE/USDA/EERE). Currently, nearly all fuel ethanol is produced from corn-derived starch. Dedicated 'energy crops' and agricultural waste are preferred long-term solutions for renewable, cheap, and globally available biofuels as they avoid some of the market pressures and secondary greenhouse gas emission challenges currently facing corn ethanol. These sources of lignocellulosic biomass are converted to fermentable sugars using a variety of chemical and thermochemical pretreatments, which disrupt cellulose and lignin cross-links, allowing exogenously added recombinant microbial enzymes to more efficiently hydrolyze the cellulose for 'deconstruction' into glucose. This process is plagued with inefficiencies, primarily due to the recalcitrance of cellulosic biomass, mass transfer issues during deconstruction, and low activity of recombinant deconstruction enzymes. Costs are also high due to the requirement for enzymes and reagents, and energy-intensive cumbersome pretreatment steps. One potential solution to these problems is found in synthetic biology-engineered plants that self-produce a suite of cellulase enzymes. Deconstruction can then be integrated into a one-step process, thereby increasing efficiency (cellulose-cellulase mass-transfer rates) and reducing costs. The unique aspects of our approach are the rationally engineered enzymes which become Trojan horses during pretreatment conditions. During this study we rationally engineered Cazy enzymes and then integrated them into plant cells by multiple transformation techniques. The regenerated plants were assayed for first expression of these messages and then for the resulting proteins. The plants were then subjected to consolidated bioprocessing and characterized in detail. Our results and possible implications of this work on developing dedicated energy crops

  19. Newly isolated and characterized bacteria with great application potential for decomposition of lignocellulosic biomass.

    PubMed

    Maki, Miranda L; Idrees, Amna; Leung, Kam Tin; Qin, Wensheng

    2012-01-01

    This study focuses on the isolation and characterization of bacteria from municipal waste and peat to determine those bacteria with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Twenty cellulase-producing bacteria belonging to four major phyla - Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes - were found when screened on carboxymethyl cellulose-containing agar. Six isolates also exhibited activities towards filter paper as the sole carbon source in salt media, while 12 exhibited activities towards xylan when screened on xylan-containing plates. Moreover, 5 isolates survived in and increased the absorbance of 1% black liquor in salt media by an average of 2.07-fold after 21 days of incubation. Similarly, these 5 isolates increased the absorbance of 0.1% pure lignin at 280 nm in salt media, indicating modification of lignin. Additionally, the Fourier transform infrared spectroscopy analysis of 1% barley straw treated for 21 days with these 5 strains showed a preference for consumption of hemicelluloses over lignin; however, a change in lignin was observed. A Bacillus strain (55S5) and a Pseudomonas strain (AS1) displayed the greatest potential for lignocellulose decomposition due to a variety of cellulase activities, as well as xylanase activity and modification of lignin. Several of these isolates have good potential for industrial use in the degradation of lignocellulosic biomass. PMID:22832891

  20. NASA's biomass production chamber: a testbed for bioregenerative life support studies

    NASA Astrophysics Data System (ADS)

    Wheeler, R. M.; Mackowiak, C. L.; Stutte, G. W.; Sager, J. C.; Yorio, N. C.; Ruffe, L. M.; Fortson, R. E.; Dreschel, T. W.; Knott, W. M.; Corey, K. A.

    1996-01-01

    The Biomass Production Chamber (BPC) located at Kennedy Space Center, FL, USA provides a large (20 m^2 area, 113 m^3 vol.), closed environment for crop growth tests for NASA's Controlled Ecological Life Support System (CELSS) program. Since the summer of 1988, the chamber has operated on a near-continuous basis (over 1200 days) without any major failures (excluding temporary power losses). During this time, five crops of wheat (64-86 days each), three crops of soybean (90 to 97 days), five crops of lettuce (28-30 days), and four crops of potato (90 to 105 days) were grown, producing 481 kg of dry plant biomass, 196 kg edible biomass, 540 kg of oxygen, 94,700 kg of condensed water, and fixing 739 kg of carbon dioxide. Results indicate that total biomass yields were close to expected values for the given light input, but edible biomass yields and harvest indices were slightly lower than expected. Stand photosynthesis, respiration, transpiration, and nutrient uptake rates were monitored throughout growth and development of the different crops, along with the build-up of ethylene and other volatile organic compounds in the atmosphere. Data were also gathered on system hardware maintenance and repair, as well as person-hours required for chamber operation. Future tests will include long-term crop production studies, tests in which nutrients from waste treatment systems will be used to grow new crops, and multi-species tests.

  1. NASA's Biomass Production Chamber: a testbed for bioregenerative life support studies.

    PubMed

    Wheeler, R M; Mackowiak, C L; Stutte, G W; Sager, J C; Yorio, N C; Ruffe, L M; Fortson, R E; Dreschel, T W; Knott, W M; Corey, K A

    1996-01-01

    The Biomass Production Chamber (BPC) located at Kennedy Space Center, FL, USA provides a large (20 m2 area, 113 m3 vol.), closed environment for crop growth tests for NASA's Controlled Ecological Life Support System (CELSS) program. Since the summer of 1988, the chamber has operated on a near-continuous basis (over 1200 days) without any major failures (excluding temporary power losses). During this time, five crops of wheat (64-86 days each), three crops of soybean (90 to 97 days), five crops of lettuce (28-30 days), and four crops of potato (90 to 105 days were grown, producing 481 kg of dry plant biomass, 196 kg edible biomass, 540 kg of oxygen, 94,700 kg of condensed water, and fixing 739 kg of carbon dioxide. Results indicate that total biomass yields were close to expected values for the given light input, but edible biomass yields and harvest indices were slightly lower than expected. Stand photosynthesis, respiration, transpiration, and nutrient uptake rates were monitored throughout growth and development of the different crops, along with the build-up of ethylene and other volatile organic compounds in the atmosphere. Data were also gathered on system hardware maintenance and repair, as well as person-hours required for chamber operation. Future tests will include long-term crop production studies, tests in which nutrients from waste treatment systems will be used to grow new crops, and multi-species tests. PMID:11538800

  2. Influence of temperature on biomass production of clones of Atriplex halimus

    NASA Astrophysics Data System (ADS)

    Dessena, Leonarda; Mulas, Maurizio

    2016-05-01

    A very effective tool to combat desertification is revegetation. Promising species for this purpose are the evergreen shrubs of the genus Atriplex. The objective of the research was to study the growing responses of Atriplex halimus under different thermal regimes and to evaluate the biomass accumulation of selected clones. The test was carried out in four sites of Sardinia Island (Italy) characterized by different latitude, altitude and air temperature trends along the year. In every site, potted plants of five clones of A. halimus were compared for biomass production as measured by linear growth of plants (central axis and secondary shoots), as well as by dry weight of leaves, shoots and roots per plant. Correlations between sums of hour-degrees under or above the thresholds of critical air temperatures, comprised between 0 and 35 °C, and the plant growth indicators were analysed. Differences among the five clones, with regard to the influence of low temperatures on plant growth and on the biomass production were evaluated. Among five tested clones, GIO1 and SAN3 resulted more sensitive to low temperatures. Clones MAR1, PAL1 and FAN3 resulted less sensitive to low temperatures and in the site characterized by the lowest minimum temperatures also have shown greater adaptability and thus biomass growth in the observed period. The clone PAL1 showed a lower shoot/root biomass ratio as adaptation to cold temperature, and the clone FAN3, the opposite behaviour and a general preference to temperate thermal regimes.

  3. Integration of waste processing and biomass production systems as part of the KSC Breadboard project.

    PubMed

    Garland, J L; Mackowiak, C L; Strayer, R F; Finger, B W

    1997-01-01

    After initial emphasis on large-scale baseline crop tests, the Kennedy Space Center (KSC) Breadboard project has begun to evaluate long-term operation of the biomass production system with increasing material closure. Our goal is to define the minimum biological processing necessary to make waste streams compatible with plant growth in hydroponic systems, thereby recycling nutrients into plant biomass and recovering water via atmospheric condensate. Initial small and intermediate-scale studies focused on the recycling of nutrients contained in inedible plant biomass. Studies conducted between 1989-1992 indicated that the majority of nutrients could be rapidly solubilized in water, but the direct use of this crop "leachate" was deleterious to plant growth due to the presence of soluble organic compounds. Subsequent studies at both the intermediate scale and in the large-scale Biomass Production Chamber (BPC) have indicated that aerobic microbiological processing of crop residue prior to incorporation into recirculating hydroponic solutions eliminated any phytotoxic effect, even when the majority of the plant nutrient demand was provided from recycled biomass during long term studies (i.e. up to 418 days). Current and future studies are focused on optimizing biological processing of both plant and human waste streams. PMID:11542556

  4. Influence of temperature on biomass production of clones of Atriplex halimus.

    PubMed

    Dessena, Leonarda; Mulas, Maurizio

    2016-05-01

    A very effective tool to combat desertification is revegetation. Promising species for this purpose are the evergreen shrubs of the genus Atriplex. The objective of the research was to study the growing responses of Atriplex halimus under different thermal regimes and to evaluate the biomass accumulation of selected clones. The test was carried out in four sites of Sardinia Island (Italy) characterized by different latitude, altitude and air temperature trends along the year. In every site, potted plants of five clones of A. halimus were compared for biomass production as measured by linear growth of plants (central axis and secondary shoots), as well as by dry weight of leaves, shoots and roots per plant. Correlations between sums of hour-degrees under or above the thresholds of critical air temperatures, comprised between 0 and 35 °C, and the plant growth indicators were analysed. Differences among the five clones, with regard to the influence of low temperatures on plant growth and on the biomass production were evaluated. Among five tested clones, GIO1 and SAN3 resulted more sensitive to low temperatures. Clones MAR1, PAL1 and FAN3 resulted less sensitive to low temperatures and in the site characterized by the lowest minimum temperatures also have shown greater adaptability and thus biomass growth in the observed period. The clone PAL1 showed a lower shoot/root biomass ratio as adaptation to cold temperature, and the clone FAN3, the opposite behaviour and a general preference to temperate thermal regimes. PMID:26353974

  5. NASA's Biomass Production Chamber: a testbed for bioregenerative life support studies

    NASA Technical Reports Server (NTRS)

    Wheeler, R. M.; Mackowiak, C. L.; Stutte, G. W.; Sager, J. C.; Yorio, N. C.; Ruffe, L. M.; Fortson, R. E.; Dreschel, T. W.; Knott, W. M.; Corey, K. A.

    1996-01-01

    The Biomass Production Chamber (BPC) located at Kennedy Space Center, FL, USA provides a large (20 m2 area, 113 m3 vol.), closed environment for crop growth tests for NASA's Controlled Ecological Life Support System (CELSS) program. Since the summer of 1988, the chamber has operated on a near-continuous basis (over 1200 days) without any major failures (excluding temporary power losses). During this time, five crops of wheat (64-86 days each), three crops of soybean (90 to 97 days), five crops of lettuce (28-30 days), and four crops of potato (90 to 105 days were grown, producing 481 kg of dry plant biomass, 196 kg edible biomass, 540 kg of oxygen, 94,700 kg of condensed water, and fixing 739 kg of carbon dioxide. Results indicate that total biomass yields were close to expected values for the given light input, but edible biomass yields and harvest indices were slightly lower than expected. Stand photosynthesis, respiration, transpiration, and nutrient uptake rates were monitored throughout growth and development of the different crops, along with the build-up of ethylene and other volatile organic compounds in the atmosphere. Data were also gathered on system hardware maintenance and repair, as well as person-hours required for chamber operation. Future tests will include long-term crop production studies, tests in which nutrients from waste treatment systems will be used to grow new crops, and multi-species tests.

  6. Integration of waste processing and biomass production systems as part of the KSC Breadboard project

    NASA Astrophysics Data System (ADS)

    Garland, J. L.; Mackowiak, C. L.; Strayer, R. F.; Finger, B. W.

    1997-01-01

    After initial emphasis on large-scale baseline crop tests, the Kennedy Space Center (KSC) Breadboard project has begun to evaluate long-term operation of the biomass production system with increasing material closure. Our goal is to define the minimum biological processing necessary to make waste streams compatible with plant growth in hydroponic systems, thereby recycling nutrients into plant biomass and recovering water via atmospheric condensate. Initial small and intermediate-scale studies focused on the recycling of nutrients contained in inedible plant biomass. Studies conducted between 1989-1992 indicated that the majority of nutrients could be rapidly solubilized in water, but the direct use of this crop ``leachate'' was deleterious to plant growth due to the presence of soluble organic compounds. Subsequent studies at both the intermediate scale and in the large-scale Biomass Production Chamber (BPC) have indicated that aerobic microbiological processing of crop residue prior to incorporation into recirculating hydroponic solutions eliminated any phytotoxic effect, even when the majority of the plant nutrient demand was provided from recycled biomass during long term studies (i.e. up to 418 days) Current and future studies are focused on optimizing biological processing of both plant and human waste streams.

  7. Biochemical conversions of lignocellulosic biomass for sustainable fuel-ethanol production in the upper Midwest

    NASA Astrophysics Data System (ADS)

    Brodeur-Campbell, Michael J.

    Biofuels are an increasingly important component of worldwide energy supply. This research aims to understand the pathways and impacts of biofuels production, and to improve these processes to make them more efficient. In Chapter 2, a life cycle assessment (LCA) is presented for cellulosic ethanol production from five potential feedstocks of regional importance to the upper Midwest — hybrid poplar, hybrid willow, switchgrass, diverse prairie grasses, and logging residues — according to the requirements of Renewable Fuel Standard (RFS). Direct land use change emissions are included for the conversion of abandoned agricultural land to feedstock production, and computer models of the conversion process are used in order to determine the effect of varying biomass composition on overall life cycle impacts. All scenarios analyzed here result in greater than 60% reduction in greenhouse gas emissions relative to petroleum gasoline. Land use change effects were found to contribute significantly to the overall emissions for the first 20 years after plantation establishment. Chapter 3 is an investigation of the effects of biomass mixtures on overall sugar recovery from the combined processes of dilute acid pretreatment and enzymatic hydrolysis. Biomass mixtures studied were aspen, a hardwood species well suited to biochemical processing; balsam, a high-lignin softwood species, and switchgrass, an herbaceous energy crop with high ash content. A matrix of three different dilute acid pretreatment severities and three different enzyme loading levels was used to characterize interactions between pretreatment and enzymatic hydrolysis. Maximum glucose yield for any species was 70% of theoretical for switchgrass, and maximum xylose yield was 99.7% of theoretical for aspen. Supplemental β-glucosidase increased glucose yield from enzymatic hydrolysis by an average of 15%, and total sugar recoveries for mixtures could be predicted to within 4% by linear interpolation of the pure

  8. Biomass and estimated production properties of size-fractionated zooplankton in the Yellow Sea, China

    NASA Astrophysics Data System (ADS)

    Huo, Yuanzi; Sun, Song; Zhang, Fang; Wang, Minxiao; Li, Chaolun; Yang, Bo

    2012-06-01

    The size-fractionated zooplankton biomass, taxonomic composition, and production calculated by formulas basing on Ikeda-Motoda's physiological methods were studied on the basis of samples taken from six cruises in the Yellow Sea. Zooplankton was size-fractionated using sieves into ~ 2 mm, 1-2 mm, 0.5-1 mm, 0.25-0.5 mm and 0.16-0.25 mm groups. The results showed that the average zooplankton biomass was 84.03 mg DM m- 3 in May, followed in order by September, June, March, August and December with 42.34, 38.36, 32.37, 27.17 and 21.83 mg DM m- 3, respectively. The contribution of ~ 2 mm, 1-2 mm, 0.5-1 mm, 0.25-0.5 mm and 0.16-0.25 mm groups to the total biomass was in the range of 15.2-27.4%, 13.2-29.4%, 14.7-18.2%, 15.8-22.6% and 16.3-34.2%, respectively, during the investigating period. The biomass of all size groups was all highest in May, and except that the biomass of 0.16-0.25 mm group was lowest in August, the biomass of other size groups was lowest in December. The dominant zooplankton species (or taxa) in each group were similar between six cruises. The estimated zooplankton production was highest in May with 1.97 mg C m- 3 d- 1, and was lowest in December with 0.51 mg C m- 3 d- 1, and was in the range of 0.67-1.44 mg C m- 3 d- 1 in other investigating months. The estimated annual zooplankton production was 0.37 g C m- 3 y- 1. The two smallest groups aggregately comprised 59-84% of the net-zooplankton production in the Yellow Sea. The geographical distribution of size-fractionated zooplankton biomass and production was significantly affected by the complex physical features of the Yellow Sea. When the Yellow Sea Cold Bottom Water appeared from June to September, the biomass and production of zooplankton larger than 1 mm were higher inside the cold water mass area than outside it, while the zooplankton smaller than 1 mm showed contrary results. The higher biomass and production of all zooplankton groups occurred in the southern part of the study area in

  9. Mid-summer mesozooplankton biomass, its size distribution, and estimated production within a glacial Arctic fjord (Hornsund, Svalbard)

    NASA Astrophysics Data System (ADS)

    Trudnowska, E.; Basedow, S. L.; Blachowiak-Samolyk, K.

    2014-09-01

    The estimation of secondary production constitutes an integrating proxy of pelagic ecosystem status, its functions as well as its responses to environmental stressors. The combination of high-resolution automatic measurements with a Laser Optical Plankton Counter (LOPC) and size spectrum analyses was utilized to estimate the secondary production of a high Arctic fjord during a summer post bloom situation in 2012. The dataset comprised 28 vertical and extensive horizontal hauls of a LOPC-CTD-fluorometer platform plus four zooplankton net sampling stations for taxonomic composition designation. A clear gradient in temperature, salinity, chlorophyll a concentrations as well as mesozooplankton abundance, biomass and production was demonstrated along Hornsund fjord axis. The outer fjord part was under the influence of water advection and had the highest chlorophyll a concentrations, numerous opaque mesozooplankton individuals and flat slopes of size spectra, pointing to long food chains in which biomass is recycled several times. The opposite state was found in the glacial bays, where the glacier meltwater discharge led to low chlorophyll a concentrations but high abundance of small and amorphous particles. It resulted in steep size spectra slopes and high intercepts implying higher potential productivity there. The model of mesozooplankton production demonstrated that Hornsund fjord is a highly productive ecosystem, particularly its upper water layer and its central parts. However, we would like to emphasize that a careful approach is needed before going deeper into ecological interpretations based on size spectra analysis, especially in reservoirs, where non-zooplankton particles contribute to the size spectra.

  10. Methods and materials for deconstruction of biomass for biofuels production

    DOEpatents

    Schoeniger, Joseph S; Hadi, Masood Zia

    2015-05-05

    The present invention relates to nucleic acids, peptides, vectors, cells, and plants useful in the production of biofuels. In certain embodiments, the invention relates to nucleic acid sequences and peptides from extremophile organisms, such as SSO1949 and Ce1A, that are useful for hydrolyzing plant cell wall materials. In further embodiments, the invention relates to modified versions of such sequences that have been optimized for production in one or both of monocot and dicot plants. In other embodiments, the invention provides for targeting peptide production or activity to a certain location within the cell or organism, such as the apoplast. In further embodiments, the invention relates to transformed cells or plants. In additional embodiments, the invention relates to methods of producing biofuel utilizing such nucleic acids, peptides, targeting sequences, vectors, cells, and/or plants.

  11. Production of biorenewable styrene: utilization of biomass-derived sugars and insights into toxicity.

    PubMed

    Lian, Jieni; McKenna, Rebekah; Rover, Marjorie R; Nielsen, David R; Wen, Zhiyou; Jarboe, Laura R

    2016-05-01

    Fermentative production of styrene from glucose has been previously demonstrated in Escherichia coli. Here, we demonstrate the production of styrene from the sugars derived from lignocellulosic biomass depolymerized by fast pyrolysis. A previously engineered styrene-producing strain was further engineered for utilization of the anhydrosugar levoglucosan via expression of levoglucosan kinase. The resulting strain produced 240 ± 3 mg L(-1) styrene from pure levoglucosan, similar to the 251 ± 3 mg L(-1) produced from glucose. When provided at a concentration of 5 g L(-1), pyrolytic sugars supported styrene production at titers similar to those from pure sugars, demonstrating the feasibility of producing this important industrial chemical from biomass-derived sugars. However, the toxicity of contaminant compounds in the biomass-derived sugars and styrene itself limit further gains in production. Styrene toxicity is generally believed to be due to membrane damage. Contrary to this prevailing wisdom, our quantitative assessment during challenge with up to 200 mg L(-1) of exogenously provided styrene showed little change in membrane integrity; membrane disruption was observed only during styrene production. Membrane fluidity was also quantified during styrene production, but no changes were observed relative to the non-producing control strain. This observation that styrene production is much more damaging to the membrane integrity than challenge with exogenously supplied styrene provides insight into the mechanism of styrene toxicity and emphasizes the importance of verifying proposed toxicity mechanisms during production instead of relying upon results obtained during exogenous challenge. PMID:26803503

  12. Photoautotrophic Production of Biomass, Laurate, and Soluble Organics by Synechocystis sp. PCC 6803

    NASA Astrophysics Data System (ADS)

    Nguyen, Binh Thanh

    Photosynthesis converts sunlight to biomass at a global scale. Among the photosynthetic organisms, cyanobacteria provide an excellent model to study how photosynthesis can become a practical platform of large-scale biotechnology. One novel approach involves metabolically engineering the cyanobacterium Synechocystis sp. PCC 6803 to excrete laurate, which is harvested directly. This work begins by defining a working window of light intensity (LI). Wild-type and laurate-excreting Synechocystis required an LI of at least 5 muE/m2-s to sustain themselves, but are photo-inhibited by LI of 346 to 598 muE/m2-s. Fixing electrons into valuable organic products, e.g., biomass and excreted laurate, is critical to success. Wild-type Synechocystis channeled 75% to 84% of its fixed electrons to biomass; laurate-excreting Synechocystis fixed 64 to 69% as biomass and 6.6% to 10% as laurate. This means that 16 to 30% of the electrons were diverted to non-valuable soluble products, and the trend was accentuated with higher LI. How the Ci concentration depended on the pH and the nitrogen source was quantified by the proton condition and experimentally validated. Nitrate increased, ammonium decreased, but ammonium nitrate stabilized alkalinity and Ci. This finding provides a mechanistically sound tool to manage Ci and pH independently. Independent evaluation pH and Ci on the growth kinetics of Synechocystis showed that pH 8.5 supported the fastest maximum specific growth rate (mumax): 2.4/day and 1.7/day, respectively, for the wild type and modified strains with LI of 202 muE/m2-s. Half-maximum-rate concentrations (KCi) were less than 0.1 mM, meaning that Synechocystis should attain its mumax with a modest Ci concentration (≥1.0 mM). Biomass grown with day-night cycles had a night endogenous decay rate of 0.05-1.0/day, with decay being faster with higher LI and the beginning of dark periods. Supplying light at a fraction of daylight reduced dark decay rate and improved overall

  13. Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process

    PubMed Central

    2013-01-01

    Background Microbial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolysates, which are then used by oleaginous microorganisms as the carbon and energy sources to produce lipids. However, the costs of microbial lipids remain prohibitively high for commercialization. More efficient and integrated processes are pivotal for better techno-economics of microbial lipid technology. Results Here we describe the simultaneous saccharification and enhanced lipid production (SSELP) process that is highly advantageous in terms of converting cellulosic materials into lipids, as it integrates cellulose biomass hydrolysis and lipid biosynthesis. Specifically, Cryptococcus curvatus cells prepared in a nutrient-rich medium were inoculated at high dosage for lipid production in biomass suspension in the presence of hydrolytic enzymes without auxiliary nutrients. When cellulose was loaded at 32.3 g/L, cellulose conversion, cell mass, lipid content and lipid coefficient reached 98.5%, 12.4 g/L, 59.9% and 204 mg/g, respectively. Lipid yields of the SSELP process were higher than those obtained by using the conventional process where cellulose was hydrolyzed separately. When ionic liquid pretreated corn stover was used, both cellulose and hemicellulose were consumed simultaneously. No xylose was accumulated over time, indicating that glucose effect was circumvented. The lipid yield reached 112 mg/g regenerated corn stover. This process could be performed without sterilization because of the absence of auxiliary nutrients for bacterial contamination. Conclusions The SSELP process facilitates direct conversion of both cellulose and hemicellulose of lignocellulosic materials into microbial lipids. It greatly reduces time and capital costs while improves lipid coefficient. Optimization of the SSELP process at different levels should further

  14. Coupling of Dimethylsulfide Oxidation to Biomass Production by a Marine Flavobacterium▿

    PubMed Central

    Green, David H.; Shenoy, Damodar M.; Hart, Mark C.; Hatton, Angela D.

    2011-01-01

    Dimethylsulfide (DMS) is an important climatically active gas. In the sea, DMS is produced primarily by microbial metabolism of the compatible solute dimethylsulfoniopropionate. Laboratory growth of Bacteroidetes with DMS resulted in its oxidation to dimethyl sulfoxide but only in the presence of glucose. We hypothesized that electrons liberated from sulfur oxidation were used to augment biomass production. PMID:21378049

  15. Nutrient removal and microalgal biomass production on urine in a short light-path photobioreactor.

    PubMed

    Tuantet, Kanjana; Temmink, Hardy; Zeeman, Grietje; Janssen, Marcel; Wijffels, René H; Buisman, Cees J N

    2014-05-15

    Due to the high nitrogen and phosphorus content, source-separated urine can serve as a major nutrient source for microalgae production. The aim of this study was to evaluate the nutrient removal rate and the biomass production rate of Chlorella sorokiniana being grown continuously in urine employing a short light-path photobioreactor. The results demonstrated, for the first time, the possibility of continuous microalgae cultivation in human urine. The lowest dilution factor successfully employed was a factor of 2 (50% v/v urine). Microalgae dominated a smaller bacterial population and were responsible for more than 90% of total nitrogen and phosphorus removal. With a light-path of 10 mm, a maximum volumetric biomass productivity as high as 9.3 g L(-1) d(-1) was achieved. The co-existing bacterial population removed up to 70% of organic pollutants from the urine at a rate of 1300 mg COD L(-1) d(-1). Enriching the urine with magnesium, adjusting the N:P molar ratio, and shortening the reactor light-path further increased the volumetric biomass productivity to 14.8 g L(-1) d(-1). The corresponding nitrogen and phosphorus removal rates were 1300 and 150 mg L(-1) d(-1), respectively. The subsequently produced biomass contained 43-53% w/w proteins and 16-25% w/w total fatty acids. PMID:24607312

  16. Biomass Program 2007 Program Peer Review - Biochemical and Products Platform Summary

    SciTech Connect

    none,

    2009-10-27

    This document discloses the comments provided by a review panel at the U.S. Department of Energy Office of the Biomass Program Peer Review held on November 15-16, 2007 in Baltimore, MD and the Biochemical and Products Platform Review held on August 7-9, 2007 in Denver, Colorado.

  17. Herbicide and Application Timing Influence Cutleaf Groundcherry Biomass and Seed Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A field experiment was conducted to evaluate herbicide and application timing on cutleaf groundcherry population, biomass, seed production, and peanut yield. Treatments included: 1) a non-treated control; 2) hand pruning; 3) diclosulam applied preemergence (PRE) at 0.027 kg ai/ha alone; 4) paraquat...

  18. Production of poly(beta-L-malic acid) (PMA) from agricultural biomass substrates by Aureobasidium pullulans

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We report here for the first time the production of poly(beta-L-malic acid) (PMA) from agricultural biomass substrates by the yeastlike fungus Aureobasidium pullulans. Strains NRRL Y 2311-1, NRRL 50382, NRRL 50383, and NRRL 50384, representing diverse isolation sources and phylogenetic clades, prod...

  19. Screening Saccharum barberi and sinense accessions for flood tolerance and biomass production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Breeding programs concentrate on S. spontaneum species to impart tolerance to environmental stresses and S. officinarum for high sugar content. Most programs are interested maximum sugar content. Little work has been done on selecting sugarcane species for biomass production. This study evaluated th...

  20. Microbial surface displayed enzymes based biofuel cell utilizing degradation products of lignocellulosic biomass for direct electrical energy.

    PubMed

    Fan, Shuqin; Hou, Chuantao; Liang, Bo; Feng, Ruirui; Liu, Aihua

    2015-09-01

    In this work, a bacterial surface displaying enzyme based two-compartment biofuel cell for the direct electrical energy conversion from degradation products of lignocellulosic biomass is reported. Considering that the main degradation products of the lignocellulose are glucose and xylose, xylose dehydrogenase (XDH) displayed bacteria (XDH-bacteria) and glucose dehydrogenase (GDH) displayed bacteria (GDH-bacteria) were used as anode catalysts in anode chamber with methylene blue as electron transfer mediator. While the cathode chamber was constructed with laccase/multi-walled-carbon nanotube/glassy-carbon-electrode. XDH-bacteria exhibited 1.75 times higher catalytic efficiency than GDH-bacteria. This assembled enzymatic fuel cell exhibited a high open-circuit potential of 0.80 V, acceptable stability and energy conversion efficiency. Moreover, the maximum power density of the cell could reach 53 μW cm(-2) when fueled with degradation products of corn stalk. Thus, this finding holds great potential to directly convert degradation products of biomass into electrical energy. PMID:26051524