Science.gov

Sample records for broadcast-sown oilseed rape

  1. NMR metabolomics of ripened and developing oilseed rape (Brassica napus) and turnip rape (Brassica rapa).

    PubMed

    Kortesniemi, Maaria; Vuorinen, Anssi L; Sinkkonen, Jari; Yang, Baoru; Rajala, Ari; Kallio, Heikki

    2015-04-01

    The oilseeds of the commercially important oilseed rape (Brassica napus) and turnip rape (Brassica rapa) were investigated with (1)H NMR metabolomics. The compositions of ripened (cultivated in field trials) and developing seeds (cultivated in controlled conditions) were compared in multivariate models using principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Differences in the major lipids and the minor metabolites between the two species were found. A higher content of polyunsaturated fatty acids and sucrose were observed in turnip rape, while the overall oil content and sinapine levels were higher in oilseed rape. The genotype traits were negligible compared to the effect of the growing site and concomitant conditions on the oilseed metabolome. This study demonstrates the applicability of NMR-based analysis in determining the species, geographical origin, developmental stage, and quality of oilseed Brassicas. PMID:25442524

  2. Development of a biologically based fertilizer, incorporating Bacillus megaterium A6, for improved phosphorus nutrition of oilseed rape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sustainable methods with diminished impact on the environment need to be developed for the production of oilseed rape in China and other regions of the world. A biological fertilizer consisting of Bacillus megaterium A6 cultured on oilseed rape meal doubled oilseed rape seed yield (P < 0.0001) rela...

  3. Formulations of Bacillus subtilis BY-2 suppress Sclerotinia sclerotiorum on oilseed rape in the field

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We are developing a collection of Bacillus strains, isolated from different environments, for use in controlling Sclerotinia sclerotiorum on oilseed rape in China and elsewhere. Strain BY-2, isolated from internal tissues of an oilseed rape root, was demonstrated to be Bacillus subtilis based on bi...

  4. Components of a Rice-Oilseed Rape Production System Augmented with Trichoderma sp. Tri-1 Control Sclerotinia sclerotiorum on Oilseed Rape.

    PubMed

    Hu, Xiaojia; Roberts, Daniel P; Xie, Lihua; Maul, Jude E; Yu, Changbing; Li, Yinshui; Zhang, Yinbo; Qin, Lu; Liao, Xing

    2015-10-01

    Sclerotinia sclerotiorum causes serious yield losses on many crops throughout the world. A multicomponent treatment that consisted of the residual rice straw remaining after rice harvest and Trichoderma sp. Tri-1 (Tri-1) formulated with the oilseed rape seedcake fertilizer was used in field soil infested with S. sclerotiorum. This treatment resulted in oilseed rape seed yield that was significantly greater than the nontreated control or when the fungicide carbendizem was used in the presence of this pathogen in field trials. Yield data suggested that the rice straw, oilseed rape seedcake, and Tri-1 components of this treatment all contributed incrementally. Similar treatment results were obtained regarding reduction in disease incidence. Slight improvements in yield and disease incidence were detected when this multicomponent treatment was combined with a fungicide spray. Inhibition of sclerotial germination by this multicomponent treatment trended greater than the nontreated control at 90, 120, and 150 days in field studies but was not significantly different from this control. This multicomponent treatment resulted in increased yield relative to the nontreated control in the absence of pathogen in a greenhouse pot study, while the straw alone and the straw plus oilseed rape seedcake treatments did not; suggesting that Tri-1 was capable of promoting growth. Experiments reported here indicate that a treatment containing components of a rice-oilseed rape production system augmented with Tri-1 can control S. sclerotiorum on oilseed rape, be used in integrated strategies containing fungicide sprays for control of this pathogen, and promote plant growth. PMID:26390095

  5. Annual dynamics of wild bee densities: attractiveness and productivity effects of oilseed rape.

    PubMed

    Riedinger, Verena; Mitesser, Oliver; Hovestadt, Thomas; Steffan-Dewenter, Ingolf; Holzschuh, Andrea

    2015-05-01

    Mass-flowering crops may affect long-term population dynamics, but effects on pollinators have never been studied across several years. We monitored wild bees in oilseed rape fields in 16 landscapes in Germany in two consecutive years. Effects on bee densities of landscape oilseed rape cover in the years of monitoring and in the previous years were evaluated with landscape data from three consecutive years. We fit empirical data to a mechanistic model to provide estimates for oilseed rape attractiveness and its effect on bee productivity in comparison to the rest of the landscape, and we evaluated consequences for pollinator densities in consecutive years. Our results show that high oilseed rape cover in the previous year enhances current densities of wild bees (except for bumble bees). Moreover, we show a strong attractiveness of and dilution on (i.e., decreasing bee densities with increasing landscape oilseed rape cover) oilseed rape for bees during flowering in the current year, modifying the effect of the previous year's oilseed rape cover in the case of wild bees (excluding Bombus). As long as other factors such as nesting sites or natural enemies do not limit bee reproduction, our findings suggest long-term positive effects of mass-flowering crops on bee populations, at least for non-Bombus generalists, which possibly help to maintain crop pollination services even when crop area increases. Similar effects are conceivable for other organisms providing ecosystem services in annual crops and should be considered in future studies. PMID:26236848

  6. Competition affects gene flow from oilseed rape (female symbol) to Brassica rapa (male symbol).

    PubMed

    Johannessen, M M; Andersen, B A; Jørgensen, R B

    2006-05-01

    Unlike most studies on hybridisation between oilseed rape and Brassica rapa, this study focused on hybridisation with oilseed rape as the maternal parent. This is a key cross because, assuming that plastids are inherited maternally, F(1)-hybrid production with maternal oilseed rape (B. napus) is the only transgene escape route from transplastomic oilseed rape. We investigated such F(1)-hybrid production in winter oilseed rape co-cultivated with weedy B. rapa at three plant densities each with two proportions of the different species. The paternity of the progeny produced on oilseed rape was assessed, and several fitness parameters were determined in oilseed rape mother plants in order to correlate hybridisation and plant competition. At higher density, the vegetative fitness per mother plant decreased significantly, but the density only affected the frequency of F(1)-hybrids significantly (a decrease) in the treatment with equal proportions of each species. As to the proportions, at higher B. napus frequencies, there were fewer F(1)-hybrids per mother plant and a significant increase in most biomass components. Thus, B. rapa was the stronger competitor in its effect on both the vegetative and reproductive fitness in B. napus, and the hybridisation frequency. In conclusion, the relative frequency of the two species was a more influential parameter than the density. Hybridisation with B. napus as the female will be most likely at current field densities of B. napus and when B. rapa is an abundant weed. PMID:16508664

  7. Transgenic oilseed rape along transportation routes and port of Vancouver in western Canada.

    PubMed

    Yoshimura, Yasuyuki; Beckie, Hugh J; Matsuo, Kazuhito

    2006-01-01

    The occurrence of transgenic herbicide-resistant oilseed rape (Brassica napus) in ruderal (non-crop disturbed) areas has not been investigated previously in Canada. The primary objective of this study was to document their occurrence in two main ruderal areas (along railways and roads) in the province of Saskatchewan, where half of all oilseed rape is grown, and at the port of Vancouver, British Columbia on the west coast of Canada, where most oilseed rape destined for export is transported by rail. During the 2005 growing season, leaf samples of oilseed rape plants were collected at randomly-selected sites along railways and roads across Saskatchewan ecoregions and at Vancouver; infestation area, density, and plant height of oilseed rape were measured at each site. The presence of the glyphosate and glufosinate resistance traits was determined using test strips. The infestation area of oilseed rape, averaged across 155 sampled sites in the Saskatchewan survey, was markedly smaller in populations along railways than roads; in contrast, infestation area averaged across 54 sites in the Vancouver survey was greater for populations along railways than roads. In both surveys, mean plant density was greater for populations found along railways than roads. Two-thirds of oilseed rape plants sampled across Saskatchewan ecoregions and at Vancouver were transgenic, although the relative proportion of plants with the glyphosate or glufosinate resistance trait varied between surveys. Frequency of occurrence of transgenic plants in ruderal areas was similar to the proportion of the oilseed rape area planted with transgenic cultivars in the recent preceding years. A single transgenic B. rapa x B. napus hybrid was found along a road in Vancouver, confirming the relatively high probability of hybridization between these two Brassica species. With current control measures, transgenic oilseed rape populations may persist and spread in these ruderal areas. PMID:17328853

  8. Feral genetically modified herbicide tolerant oilseed rape from seed import spills: are concerns scientifically justified?

    PubMed

    Devos, Yann; Hails, Rosemary S; Messéan, Antoine; Perry, Joe N; Squire, Geoffrey R

    2012-02-01

    One of the concerns surrounding the import (for food and feed uses or processing) of genetically modified herbicide tolerant (GMHT) oilseed rape is that, through seed spillage, the herbicide tolerance (HT) trait will escape into agricultural or semi-natural habitats, causing environmental or economic problems. Based on these concerns, three EU countries have invoked national safeguard clauses to ban the marketing of specific GMHT oilseed rape events on their territory. However, the scientific basis for the environmental and economic concerns posed by feral GMHT oilseed rape resulting from seed import spills is debatable. While oilseed rape has characteristics such as secondary dormancy and small seed size that enable it to persist and be redistributed in the landscape, the presence of ferals is not in itself an environmental or economic problem. Crucially, feral oilseed rape has not become invasive outside cultivated and ruderal habitats, and HT traits are not likely to result in increased invasiveness. Feral GMHT oilseed rape has the potential to introduce HT traits to volunteer weeds in agricultural fields, but would only be amplified if the herbicides to which HT volunteers are tolerant were used routinely in the field. However, this worst-case scenario is most unlikely, as seed import spills are mostly confined to port areas. Economic concerns revolve around the potential for feral GMHT oilseed rape to contribute to GM admixtures in non-GM crops. Since feral plants derived from cultivation (as distinct from import) occur at too low a frequency to affect the coexistence threshold of 0.9% in the EU, it can be concluded that feral GMHT plants resulting from seed import spills will have little relevance as a potential source of pollen or seed for GM admixture. This paper concludes that feral oilseed rape in Europe should not be routinely managed, and certainly not in semi-natural habitats, as the benefits of such action would not outweigh the negative effects of

  9. Components of a rice-oilseed rape production system augmented with trichoderma sp. Tri-1 control sclerotinia sclerotiorum on oilseed rape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sclerotinia sclerotiorum causes serious yield losses on many crops throughout the world. In two field trials conducted at the same location in consecutive years, a treatment containing formulated Trichoderma harzianum-1 (Tri-1) resulted in oilseed rape seed yield that was significantly greater than...

  10. Low level impurities in imported wheat are a likely source of feral transgenic oilseed rape (Brassica napus L.) in Switzerland.

    PubMed

    Schulze, Juerg; Brodmann, Peter; Oehen, Bernadette; Bagutti, Claudia

    2015-11-01

    In Switzerland, the cultivation of genetically modified (GM) oilseed rape (Brassica napus L.) and the use of its seeds for food and feed are not permitted. Nevertheless, the GM oilseed rape events GT73, MS8×RF3, MS8 and RF3 have recently been found in the Rhine port of Basel, Switzerland. The sources of GM oilseed rape seeds have been unknown. The main agricultural good being imported at the Rhine port of Basel is wheat and from 2010 to 2013, 19% of all Swiss wheat imports originated from Canada. As over 90% of all oilseed rape grown in Canada is GM, we hypothesised that imports of Canadian wheat may contain low level impurities of GM oilseed rape. Therefore, waste fraction samples gathered during the mechanical cleaning of Canadian wheat from two Swiss grain mills were analysed by separating oilseed rape seeds from waste fraction samples and testing DNA of pooled seeds for the presence of transgenes by real-time PCR. Furthermore, oilseed rape seeds from each grain mill were sown in a germination experiment, and seedling DNA was tested for the presence of transgenes by real-time PCR. GT73, MS8×RF3, MS8 and RF3 oilseed rape was detected among seed samples and seedlings of both grain mills. Based on this data, we projected a mean proportion of 0.005% of oilseed rape in wheat imported from Canada. Besides Canadian wheat, the Rhine port of Basel does not import any other significant amounts of agricultural products from GM oilseed rape producing countries. We therefore conclude that Canadian wheat is the major source of unintended introduction of GM oilseed rape seeds into Switzerland. PMID:26109224

  11. Evaluation of the Minolta SPAD-502 meter for nitrogen management of oilseed rape

    NASA Astrophysics Data System (ADS)

    Zhu, Zhe-yan; He, Yong; Huang, Min

    2006-09-01

    Nitrogen is important for oilseed rape production and even moderate deficiencies will substantially reduce yield and profit, but excessive N can pollute both surface and ground water. Thus, farmers are walking a thin line. In order to find the relationship between the SPAD value and the nitrogen content of oilseed rapes, a Minolta SPAD-502 Meter can be used for on-farm measurement of the N content, and then the Kjeldahl method has been used for measuring the nitrogen content of these oilseed rapes. Every leaf had 10 points SPAD value in average for measuring by SPAD, according to the area of the leaves, the points were increased or decreased. The Kjeldahl method has been used for organic nitrogen determination for over a century. Before using the Kjeldahl method to measuring the nitrogen content, all the leaves has been washed and dried. The results of these two methods has been fitted, as a result, the correlation coefficient is 0.863. Another sample was used to check if the result was perfect, the error of the forecasting value is about 7.4%. A new method for measuring the nitrogen content quickly of oilseed rapes has been found, the nitrogen content of the oilseed rape can be measured nondestructively and quickly.

  12. Monitoring the occurrence of genetically modified oilseed rape growing along a Japanese roadside: 3-year observations.

    PubMed

    Nishizawa, Toru; Nakajima, Nobuyoshi; Aono, Mitsuko; Tamaoki, Masanori; Kubo, Akihiro; Saji, Hikaru

    2009-01-01

    Monitoring for escape of genetically modified (GM) oilseed rape (Brassica napus) during transport can be performed by means of roadside evaluations in areas where cultivation of this GM crop is not conducted, such as in Japan. We performed a survey of oilseed rape plants growing along a 20-km section of Japan's Route 51, one of the main land transportation routes in central Japan for imports of GM oilseed rape from the Port of Kashima into Keiyo District. Oilseed rape plants were found each year, but the number of plants varied substantially during the three years of our study: 2162 plants in 2005, 4066 in 2006, and only 278 in 2007. The low number in 2007 was probably caused by roadwork. Herbicide-resistant individuals were detected in the three consecutive years (26, 8, and 5 individuals with glyphosate resistance), but glufosinate-resistant plants (9 individuals) were detected only in 2005. The roadside plants occurred mainly along the inbound lane from Kashima to Narita. These plants are likely to have their origin in seeds spilled during transportation of cargo from the port, since there are no potential natural seed source plants for B. napus near Route 51. This is the first detailed report on the transition and distribution of herbicide-resistant oilseed rape plants following loss and spillage along Japanese roads. PMID:19419652

  13. Seed architecture shapes embryo metabolism in oilseed rape.

    PubMed

    Borisjuk, Ljudmilla; Neuberger, Thomas; Schwender, Jörg; Heinzel, Nicolas; Sunderhaus, Stephanie; Fuchs, Johannes; Hay, Jordan O; Tschiersch, Henning; Braun, Hans-Peter; Denolf, Peter; Lambert, Bart; Jakob, Peter M; Rolletschek, Hardy

    2013-05-01

    Constrained to develop within the seed, the plant embryo must adapt its shape and size to fit the space available. Here, we demonstrate how this adjustment shapes metabolism of photosynthetic embryo. Noninvasive NMR-based imaging of the developing oilseed rape (Brassica napus) seed illustrates that, following embryo bending, gradients in lipid concentration became established. These were correlated with the local photosynthetic electron transport rate and the accumulation of storage products. Experimentally induced changes in embryo morphology and/or light supply altered these gradients and were accompanied by alterations in both proteome and metabolome. Tissue-specific metabolic models predicted that the outer cotyledon and hypocotyl/radicle generate the bulk of plastidic reductant/ATP via photosynthesis, while the inner cotyledon, being enclosed by the outer cotyledon, is forced to grow essentially heterotrophically. Under field-relevant high-light conditions, major contribution of the ribulose-1,5-bisphosphate carboxylase/oxygenase-bypass to seed storage metabolism is predicted for the outer cotyledon and the hypocotyl/radicle only. Differences between in vitro- versus in planta-grown embryos suggest that metabolic heterogeneity of embryo is not observable by in vitro approaches. We conclude that in vivo metabolic fluxes are locally regulated and connected to seed architecture, driving the embryo toward an efficient use of available light and space. PMID:23709628

  14. Critical period of weed control in oilseed rape in two Moroccan regions.

    PubMed

    Maataoui, A; Bouhache, M; Benbella, M; Talouizte, A

    2003-01-01

    The determination of critical period of weed control in oilseed rape is necessary to know the weed control period. To determine the critical period, two fields experiments were carried out during 1995-96 growth season in Loukkos and Saïs regions at two oilseed densities (D1 = 24 and D2 = 36 plants m(-2)). Ten treatments corresponding to plots left weed free or weeded plots until four leaves, flowers bud, flowering, puds formation, and maturity stages of oilseed rape were tested. Density and biomass of weeds were determined at each oilseed stages. Results showed that weed density and biomass were higher in Saïs than in Loukkos sites. For a 10% yield loss, critical period of weed control in Loukkos was from 458 to 720 degree days after emergence (D degrees AE) and from 480 to 720 D degrees AE in oilseed conducted at densities D1 and D2, respectively. In Saïs, critical period of weed control was from 474 to 738 D degrees AE and from 468 to 675 D degrees AE in oilseed conducted at D1 and D2, respectively. It was concluded that the length of the critical period of weed control in oilseed rape grain yield seems to be dependant of the level of the infestation. PMID:15149131

  15. In-field frequencies and characteristics of oilseed rape with double herbicide resistance.

    PubMed

    Dietz-Pfeilstetter, Antje; Zwerger, Peter

    2009-01-01

    When growing different transgenic herbicide-resistant oilseed rape cultivars side by side, seeds with multiple herbicide resistance can arise, possibly causing problems for the management of volunteer plants. Large-scale field experiments were performed in the years 1999/2000 and 2000/2001 in order to investigate the frequencies and the consequences of the transfer of herbicide resistance genes from transgenic oilseed rape to cultivars grown on neighboring agricultural fields. Transgenic oilseed rape with resistance to glufosinate-ammonium (LibertyLink, LL) and with glyphosate resistance (RoundupReady, RR), respectively, was sown in adjacent 0.5 ha plots, surrounded by about 8 ha non-transgenic oilseed rape. The plots and the field were either in direct contact (0.5 m gap width) or they were separated by 10 m of fallow land. Seed samples taken during harvest in the transgenic plots at different distances were investigated for progeny with resistance to the respective other herbicide. It was found that outcrossing frequencies were reduced to different extents by a 10 m isolation distance. In addition to pollen-mediated transgene flow as a result of outcrossing, we found considerable seed-mediated gene flow by adventitious dispersal of transgenic seeds through the harvesting machine. Volunteer plants with double herbicide resistance emerging in the transgenic plots after harvest were selected by suitable applications of the complementary herbicides Basta and Roundup Ultra. In both years, double-resistant volunteers were largely restricted to the inner edges of the plots. Expression analysis under controlled laboratory conditions of double-resistant plants generated by manual crosses revealed stability of transgene expression even at elevated temperatures. Greenhouse tests with double-resistant oilseed rape plants gave no indication that the sensitivity to a range of different herbicides is changed as compared to non-transgenic oilseed rape. PMID:19833077

  16. Specifics of soil temperature under winter oilseed rape canopy

    NASA Astrophysics Data System (ADS)

    Krčmářová, Jana; Středa, Tomáš; Pokorný, Radovan

    2014-09-01

    The aim of this study was to evaluate the course of soil temperature under the winter oilseed rape canopy and to determine relationships between soil temperature, air temperature and partly soil moisture. In addition, the aim was to describe the dependence by means of regression equations usable for pests and pathogens prediction, crop development, and yields models. The measurement of soil and near the ground air temperatures was performed at the experimental field Žabiče (South Moravia, the Czech Republic). The course of temperature was determined under or in the winter oilseed rape canopy during spring growth season in the course of four years (2010 - 2012 and 2014). In all years, the standard varieties (Petrol, Sherpa) were grown, in 2014 the semi-dwarf variety PX104 was added. Automatic soil sensors were positioned at three depths (0.05, 0.10 and 0.20 m) under soil surface, air temperature sensors in 0.05 m above soil surfaces. The course of soil temperature differs significantly between standard (Sherpa and Petrol) and semi-dwarf (PX104) varieties. Results of the cross correlation analysis showed, that the best interrelationships between air and soil temperature were achieved in 2 hours delay for the soil temperature in 0.05 m, 4 hour delay for 0.10 m and 7 hour delay for 0.20 m for standard varieties. For semi-dwarf variety, this delay reached 6 hour for the soil temperature in 0.05 m, 7 hour delay for 0.10 m and 11 hour for 0.20 m. After the time correction, the determination coefficient (R2) reached values from 0.67 to 0.95 for 0.05 m, 0.50 to 0.84 for 0.10 m in variety Sherpa during all experimental years. For variety PX104 this coefficient reached values from 0.51 to 0.72 in 0.05 m depth and from 0.39 to 0.67 in 0.10 m depth in the year 2014. The determination coefficient in the 0.20 m depth was lower for both varieties; its values were from 0.15 to 0.65 in variety Sherpa. In variety PX104 the values of R2 from 0.23 to 0.57 were determined. When using

  17. Early osmotic adjustment responses in drought-resistant and drought-sensitive oilseed rape.

    PubMed

    Hatzig, Sarah; Zaharia, L Irina; Abrams, Suzanne; Hohmann, Marie; Legoahec, Laurie; Bouchereau, Alain; Nesi, Nathalie; Snowdon, Rod J

    2014-08-01

    The impact of osmotic stress on growth, physiology, and metabolism of winter oilseed rape (Brassica napus L.) was investigated by detailed analysis of biomass traits, hormone metabolites and osmolytes in two genetically unrelated drought-tolerant genotypes and two unrelated drought-sensitive genotypes. Seedlings were grown in vitro under controlled conditions and osmotic stress was simulated by applying a gradual treatment with polyethylene glycol (PEG 6000), followed by hypo-osmotic treatment of variants used for metabolite determination. The results provide a basis for the identification of reliable selection criteria for drought resistance in oilseed rape. The in vitro cultivation system established during this study enabled effective discrimination of early osmotic stress responses between drought-resistant and -susceptible oilseed rape genotypes that also show large differences in relative seed yield under drought conditions in the field. Clear physiological and metabolic differences were observed between the drought-resistant and drought-sensitive genotypes, suggesting that osmotic adjustment is a key component of drought response in oilseed rape. Unexpectedly, however, the drought-resistant genotypes did not show typical hormonal adjustment and osmolyte accumulation, suggesting that they possess alternative physiological mechanisms enabling avoidance of stress symptoms. PMID:24667002

  18. Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape During In Vitro Culture

    SciTech Connect

    Schwender, Jorg; Hebbelmann, Inga; Heinzel, Nicholas; Hildebrandt, Tatjana; Rogers, Alistair; Naik, Dhiraj; Klapperstuck, Matthias; Braun, Hans -Peter; Schreiber, Falk; Denolf, Peter; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2015-07-01

    Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. We observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Also, quantitative data were used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3',5'-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism..

  19. Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture.

    PubMed

    Schwender, Jörg; Hebbelmann, Inga; Heinzel, Nicolas; Hildebrandt, Tatjana; Rogers, Alistair; Naik, Dhiraj; Klapperstück, Matthias; Braun, Hans-Peter; Schreiber, Falk; Denolf, Peter; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2015-07-01

    Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. Overall, we observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Quantitative data were also used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3',5'-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism. PMID:25944824

  20. Assessing winter oilseed rape freeze injury based on Chinese HJ remote sensing data*

    PubMed Central

    She, Bao; Huang, Jing-feng; Guo, Rui-fang; Wang, Hong-bin; Wang, Jing

    2015-01-01

    The winter oilseed rape (Brassica napus L.) accounts for about 90% of the total acreage of oilseed rape in China. However, it suffers the risk of freeze injury during the winter. In this study, we used Chinese HJ-1A/1B CCD sensors, which have a revisit frequency of 2 d as well as 30 m spatial resolution, to monitor the freeze injury of oilseed rape. Mahalanobis distance-derived growing regions in a normal year were taken as the benchmark, and a mask method was applied to obtain the growing regions in the 2010–2011 growing season. The normalized difference vegetation index (NDVI) was chosen as the indicator of the degree of damage. The amount of crop damage was determined from the difference in the NDVI before and after the freeze. There was spatial variability in the amount of crop damage, so we examined three factors that may affect the degree of freeze injury: terrain, soil moisture, and crop growth before the freeze. The results showed that all these factors were significantly correlated with freeze injury degree (P<0.01, two-tailed). The damage was generally more serious in low-lying and drought-prone areas; in addition, oilseed rape planted on south- and west-oriented facing slopes and those with luxuriant growth status tended to be more susceptible to freeze injury. Furthermore, land surface temperature (LST) of the coldest day, soil moisture, pre-freeze growth and altitude were in descending order of importance in determining the degree of damage. The findings proposed in this paper would be helpful in understanding the occurrence and severity distribution of oilseed rape freeze injury under certain natural or vegetation conditions, and thus help in mitigation of this kind of meteorological disaster in southern China. PMID:25644468

  1. Assessing winter oilseed rape freeze injury based on Chinese HJ remote sensing data.

    PubMed

    She, Bao; Huang, Jing-feng; Guo, Rui-fang; Wang, Hong-bin; Wang, Jing

    2015-02-01

    The winter oilseed rape (Brassica napus L.) accounts for about 90% of the total acreage of oilseed rape in China. However, it suffers the risk of freeze injury during the winter. In this study, we used Chinese HJ-1A/1B CCD sensors, which have a revisit frequency of 2 d as well as 30 m spatial resolution, to monitor the freeze injury of oilseed rape. Mahalanobis distance-derived growing regions in a normal year were taken as the benchmark, and a mask method was applied to obtain the growing regions in the 2010-2011 growing season. The normalized difference vegetation index (NDVI) was chosen as the indicator of the degree of damage. The amount of crop damage was determined from the difference in the NDVI before and after the freeze. There was spatial variability in the amount of crop damage, so we examined three factors that may affect the degree of freeze injury: terrain, soil moisture, and crop growth before the freeze. The results showed that all these factors were significantly correlated with freeze injury degree (P<0.01, two-tailed). The damage was generally more serious in low-lying and drought-prone areas; in addition, oilseed rape planted on south- and west-oriented facing slopes and those with luxuriant growth status tended to be more susceptible to freeze injury. Furthermore, land surface temperature (LST) of the coldest day, soil moisture, pre-freeze growth and altitude were in descending order of importance in determining the degree of damage. The findings proposed in this paper would be helpful in understanding the occurrence and severity distribution of oilseed rape freeze injury under certain natural or vegetation conditions, and thus help in mitigation of this kind of meteorological disaster in southern China. PMID:25644468

  2. Multi range spectral feature fitting for hyperspectral imagery in extracting oilseed rape planting area

    NASA Astrophysics Data System (ADS)

    Pan, Zhuokun; Huang, Jingfeng; Wang, Fumin

    2013-12-01

    Spectral feature fitting (SFF) is a commonly used strategy for hyperspectral imagery analysis to discriminate ground targets. Compared to other image analysis techniques, SFF does not secure higher accuracy in extracting image information in all circumstances. Multi range spectral feature fitting (MRSFF) from ENVI software allows user to focus on those interesting spectral features to yield better performance. Thus spectral wavelength ranges and their corresponding weights must be determined. The purpose of this article is to demonstrate the performance of MRSFF in oilseed rape planting area extraction. A practical method for defining the weighted values, the variance coefficient weight method, was proposed to set up criterion. Oilseed rape field canopy spectra from the whole growth stage were collected prior to investigating its phenological varieties; oilseed rape endmember spectra were extracted from the Hyperion image as identifying samples to be used in analyzing the oilseed rape field. Wavelength range divisions were determined by the difference between field-measured spectra and image spectra, and image spectral variance coefficient weights for each wavelength range were calculated corresponding to field-measured spectra from the closest date. By using MRSFF, wavelength ranges were classified to characterize the target's spectral features without compromising spectral profile's entirety. The analysis was substantially successful in extracting oilseed rape planting areas (RMSE ≤ 0.06), and the RMSE histogram indicated a superior result compared to a conventional SFF. Accuracy assessment was based on the mapping result compared with spectral angle mapping (SAM) and the normalized difference vegetation index (NDVI). The MRSFF yielded a robust, convincible result and, therefore, may further the use of hyperspectral imagery in precision agriculture.

  3. Unexpected Diversity of Feral Genetically Modified Oilseed Rape (Brassica napus L.) Despite a Cultivation and Import Ban in Switzerland

    PubMed Central

    Schulze, Juerg; Frauenknecht, Tina; Brodmann, Peter; Bagutti, Claudia

    2014-01-01

    Despite cultivation and seed import bans of genetically modified (GM) oilseed rape (Brassica napus L.), feral GM plants were found growing along railway lines and in port areas at four sites in Switzerland in 2011 and 2012. All GM plants were identified as glyphosate-resistant GM event GT73 (Roundup Ready, Monsanto). The most affected sites were the Rhine port of Basel and the St. Johann freight railway station in Basel. To assess the distribution and intra- and interspecific outcrossing of GM oilseed rape in more detail, we monitored these two sites in 2013. Leaves and seed pods of feral oilseed rape plants, their possible hybridization partners and putative hybrid plants were sampled in monthly intervals and analysed for the presence of transgenes by real-time PCR. Using flow cytometry, we measured DNA contents of cell nuclei to confirm putative hybrids. In total, 2787 plants were sampled. The presence of GT73 oilseed rape could be confirmed at all previously documented sampling locations and was additionally detected at one new sampling location within the Rhine port. Furthermore, we found the glufosinate-resistant GM events MS8xRF3, MS8 and RF3 (all traded as InVigor, Bayer) at five sampling locations in the Rhine port. To our knowledge, this is the first time that feral MS8xRF3, MS8 or RF3 plants were detected in Europe. Real-time PCR analyses of seeds showed outcrossing of GT73 into two non-GM oilseed rape plants, but no outcrossing of transgenes into related wild species was observed. We found no hybrids between oilseed rape and related species. GM plants most frequently occurred at unloading sites for ships, indicating that ship cargo traffic is the main entry pathway for GM oilseed rape. In the future, it will be of major interest to determine the source of GM oilseed rape seeds. PMID:25464509

  4. Unexpected diversity of feral genetically modified oilseed rape (Brassica napus L.) despite a cultivation and import ban in Switzerland.

    PubMed

    Schulze, Juerg; Frauenknecht, Tina; Brodmann, Peter; Bagutti, Claudia

    2014-01-01

    Despite cultivation and seed import bans of genetically modified (GM) oilseed rape (Brassica napus L.), feral GM plants were found growing along railway lines and in port areas at four sites in Switzerland in 2011 and 2012. All GM plants were identified as glyphosate-resistant GM event GT73 (Roundup Ready, Monsanto). The most affected sites were the Rhine port of Basel and the St. Johann freight railway station in Basel. To assess the distribution and intra- and interspecific outcrossing of GM oilseed rape in more detail, we monitored these two sites in 2013. Leaves and seed pods of feral oilseed rape plants, their possible hybridization partners and putative hybrid plants were sampled in monthly intervals and analysed for the presence of transgenes by real-time PCR. Using flow cytometry, we measured DNA contents of cell nuclei to confirm putative hybrids. In total, 2787 plants were sampled. The presence of GT73 oilseed rape could be confirmed at all previously documented sampling locations and was additionally detected at one new sampling location within the Rhine port. Furthermore, we found the glufosinate-resistant GM events MS8xRF3, MS8 and RF3 (all traded as InVigor, Bayer) at five sampling locations in the Rhine port. To our knowledge, this is the first time that feral MS8xRF3, MS8 or RF3 plants were detected in Europe. Real-time PCR analyses of seeds showed outcrossing of GT73 into two non-GM oilseed rape plants, but no outcrossing of transgenes into related wild species was observed. We found no hybrids between oilseed rape and related species. GM plants most frequently occurred at unloading sites for ships, indicating that ship cargo traffic is the main entry pathway for GM oilseed rape. In the future, it will be of major interest to determine the source of GM oilseed rape seeds. PMID:25464509

  5. Challenges to grow oilseed rape Brassica napus in sugar beet rotations.

    PubMed

    Stefanovska, T; Pidlisnyuk, V

    2009-01-01

    The study was carried out in 1989-1991 and repeated in 2003-2006 to compare life cycle and dynamics of Heterodera schachtii Schm. on sugar beet, oilseed rape, fodder radish and to work out recommendations on how to decrease the risk of yield reduction while it grows in sugar-beet rotations. Research was carried out in plot experiment in natural conditions. Nematode community on rape, fodder radish and sugar beet was analyzed. Data of nematode community showed that composition of nematode species was very similar. Heterodera shachtii were dominated species with rape and sugar beet. All tested Brassica crops are susceptible to H. schachtii. However there is significant difference in population dynamics. The highest total number of brown cysts, eggs and juveniles of all ages was observed in winter rape. H.schachtii developed two generations on sugar beet and one generation on mustard. The voluntary seed germination after harvest contributes to increasing H. schachtii population. Therefore it is necessary to destroy oilseed rape voluntary chemically or physically. This operation should be done in about 2-4 weeks. The exact time can be calculated using the temperature- based model. Growing regular fodder radish and mustard as the trap crops can significantly reduce population of H. schachtii. The time of sowing is not earlier than August 20th. While estimating the time of destruction of trap crops it should be taken into consideration that H. schachtii can complete life cycle without foliage. PMID:20222620

  6. Large-scale pollination experiment demonstrates the importance of insect pollination in winter oilseed rape.

    PubMed

    Lindström, Sandra A M; Herbertsson, Lina; Rundlöf, Maj; Smith, Henrik G; Bommarco, Riccardo

    2016-03-01

    Insect pollination, despite its potential to contribute substantially to crop production, is not an integrated part of agronomic planning. A major reason for this are knowledge gaps in the contribution of pollinators to yield, which partly result from difficulties in determining area-based estimates of yield effects from insect pollination under field conditions. We have experimentally manipulated honey bee Apis mellifera densities at 43 oilseed rape Brassica napus fields over 2 years in Scandinavia. Honey bee hives were placed in 22 fields; an additional 21 fields without large apiaries in the surrounding landscape were selected as controls. Depending on the pollination system in the parental generation, the B. napus cultivars in the crop fields are classified as either open-pollinated or first-generation hybrids, with both types being open-pollinated in the generation of plants cultivated in the fields. Three cultivars of each type were grown. We measured the activity of flower-visiting insects during flowering and estimated yields by harvesting with small combine harvesters. The addition of honey bee hives to the fields dramatically increased abundance of flower-visiting honey bees in those fields. Honey bees affected yield, but the effect depended on cultivar type (p = 0.04). Post-hoc analysis revealed that open-pollinated cultivars, but not hybrid cultivars, had 11% higher yields in fields with added honey bees than those grown in the control fields (p = 0.07). To our knowledge, this is the first whole-field study in replicated landscapes to assess the benefit of insect pollination in oilseed rape. Our results demonstrate that honey bees have the potential to increase oilseed rape yields, thereby emphasizing the importance of pollinator management for optimal cultivation of oilseed rape. PMID:26650584

  7. Cold plasma treatment enhances oilseed rape seed germination under drought stress

    PubMed Central

    Ling, Li; Jiangang, Li; Minchong, Shen; Chunlei, Zhang; Yuanhua, Dong

    2015-01-01

    Effects of cold plasma treatment on seed germination, seedling growth, antioxidant enzymes, lipid peroxidation levels and osmotic-adjustment products of oilseed rape under drought stress were investigated in a drought-sensitive (Zhongshuang 7) and drought-tolerant cultivar (Zhongshuang 11). Results showed that, under drought stress, cold plasma treatment significantly improved the germination rate by 6.25% in Zhongshuang 7, and 4.44% in Zhongshuang 11. Seedling growth characteristics, including shoot and root dry weights, shoot and root lengths, and lateral root number, significantly increased after cold plasma treatment. The apparent contact angle was reduced by 30.38% in Zhongshuang 7 and 16.91% in Zhongshuang 11. Cold plasma treatment markedly raised superoxide dismutase and catalase activities by 17.71% and 16.52% in Zhongshuang 7, and by 13.00% and 13.21% in Zhongshuang 11. Moreover, cold plasma treatment significantly increased the soluble sugar and protein contents, but reduced the malondialdehyde content in seedlings. Our results suggested that cold plasma treatment improved oilseed rape drought tolerance by improving antioxidant enzyme activities, increasing osmotic-adjustment products, and reducing lipid peroxidation, especially in the drought-sensitive cultivar (Zhongshuang 7). Thus, cold plasma treatment can be used in an ameliorative way to improve germination and protect oilseed rape seedlings against damage caused by drought stress. PMID:26264651

  8. Response of seed tocopherols in oilseed rape to nitrogen fertilizer sources and application rates* #

    PubMed Central

    Hussain, Nazim; Li, Hui; Jiang, Yu-xiao; Jabeen, Zahra; Shamsi, Imran Haider; Ali, Essa; Jiang, Li-xi

    2014-01-01

    Tocopherols (Tocs) are vital scavengers of reactive oxygen species (ROS) and important seed oil quality indicators. Nitrogen (N) is one of the most important fertilizers in promoting biomass and grain yield in crop production. However, the effect of different sources and application rates of N on seed Toc contents in oilseed rape is poorly understood. In this study, pot trials were conducted to evaluate the effect of two sources of N fertilizer (urea and ammonium nitrate). Each source was applied to five oilseed rape genotypes (Zheshuang 72, Jiu-Er-1358, Zheshuang 758, Shiralee, and Pakola) at three different application rates (0.41 g/pot (N1), 0.81 g/pot (N2), and 1.20 g/pot (N3)). Results indicated that urea increased α-, γ-, and total Toc (T-Toc) more than did ammonium nitrate. N3 was proven as the most efficient application rate, which yielded high contents of γ-Toc and T-Toc. Highly significant correlations were observed between Toc isomers, T-Toc, and α-/γ-Toc ratio. These results clearly demonstrate that N sources and application rates significantly affect seed Toc contents in oilseed rape. PMID:24510711

  9. Cold plasma treatment enhances oilseed rape seed germination under drought stress.

    PubMed

    Ling, Li; Jiangang, Li; Minchong, Shen; Chunlei, Zhang; Yuanhua, Dong

    2015-01-01

    Effects of cold plasma treatment on seed germination, seedling growth, antioxidant enzymes, lipid peroxidation levels and osmotic-adjustment products of oilseed rape under drought stress were investigated in a drought-sensitive (Zhongshuang 7) and drought-tolerant cultivar (Zhongshuang 11). Results showed that, under drought stress, cold plasma treatment significantly improved the germination rate by 6.25% in Zhongshuang 7, and 4.44% in Zhongshuang 11. Seedling growth characteristics, including shoot and root dry weights, shoot and root lengths, and lateral root number, significantly increased after cold plasma treatment. The apparent contact angle was reduced by 30.38% in Zhongshuang 7 and 16.91% in Zhongshuang 11. Cold plasma treatment markedly raised superoxide dismutase and catalase activities by 17.71% and 16.52% in Zhongshuang 7, and by 13.00% and 13.21% in Zhongshuang 11. Moreover, cold plasma treatment significantly increased the soluble sugar and protein contents, but reduced the malondialdehyde content in seedlings. Our results suggested that cold plasma treatment improved oilseed rape drought tolerance by improving antioxidant enzyme activities, increasing osmotic-adjustment products, and reducing lipid peroxidation, especially in the drought-sensitive cultivar (Zhongshuang 7). Thus, cold plasma treatment can be used in an ameliorative way to improve germination and protect oilseed rape seedlings against damage caused by drought stress. PMID:26264651

  10. Detection of feral transgenic oilseed rape with multiple-herbicide resistance in Japan.

    PubMed

    Aono, Mitsuko; Wakiyama, Seiji; Nagatsu, Masato; Nakajima, Nobuyoshi; Tamaoki, Masanori; Kubo, Akihiro; Saji, Hikaru

    2006-01-01

    Repeated monitoring for escaped transgenic crop plants is sometimes necessary, especially in cases when the crop has not been approved for release into the environment. Transgenic oilseed rape (Brassica napus) was detected along roadsides in central Japan in a previous study. The goal of the current study was to monitor the distribution of transgenic oilseed rape and occurrence of hybridization of transgenic B. napus with feral populations of its closely related species (B. rapa and B. juncea) in the west of Japan in 2005. The progenies of 50 B. napus, 82 B. rapa and 283 B. juncea maternal plants from 95 sampling sites in seven port areas were screened for herbicide-resistance. Transgenic herbicide-resistant seeds were detected from 12 B. napus maternal plants growing at seven sampling sites in two port areas. A portion of the progeny from two transgenic B. napus plants had both glyphosate-resistance and glufosinate-resistance transgenes. Therefore, two types of transgenic B. napus plants are likely to have outcrossed with each other, since the double-herbicide-resistant transgenic strain of oilseed rape has not been developed intentionally for commercial purposes. As found in the previous study, no transgenic seeds were detected from B. rapa or B. juncea, and more extensive sampling is needed to determine whether introgression into these wild species has occurred. PMID:17328854

  11. Persistence of seeds from crops of conventional and herbicide tolerant oilseed rape (Brassica napus).

    PubMed

    Lutman, Peter J W; Berry, Kate; Payne, Roger W; Simpson, Euan; Sweet, Jeremy B; Champion, Gillian T; May, Mike J; Wightman, Pat; Walker, Kerr; Lainsbury, Martin

    2005-09-22

    A series of rotation experiments at five sites over four years has explored the environmental and agronomic implications of growing herbicide tolerant oilseed rape and sugar beet. This paper reports on the population dynamics of volunteer rape (Brassica napus). The experiments compared four winter oilseed rape (WOSR) cultivars: a conventional cultivar (Apex) and three developmental cultivars either genetically modified (GM) to be tolerant to glyphosate or glufosinate, or conventionally bred to be tolerant to herbicides of the imidazolinone group. Seed losses at harvest averaged 3575 seeds m(-2) but ranged from less than 2000 up to more than 10000 seeds m(-2). There was a rapid decline in seed numbers during the first few months after harvest, resulting in a mean loss of seeds of 60%. In subsequent seasons, the seedbank declined much more slowly at four of the five sites (ca 20% per year) and the models predicted 95% seed loss after approximately 9 years. Seed decline was much faster at the fifth site. There were no clear differences between the four cultivars in either the numbers of seeds shed at harvest or in their subsequent persistence. The importance of the persistence of GM rape seeds, in the context of the coexistence of GM and non-GM crops and the role of good management practices that minimize seed persistence, are discussed. PMID:16191596

  12. Ozone dose-response relationships for spring oilseed rape and broccoli

    NASA Astrophysics Data System (ADS)

    De Bock, Maarten; Op de Beeck, Maarten; De Temmerman, Ludwig; Guisez, Yves; Ceulemans, Reinhart; Vandermeiren, Karine

    2011-03-01

    Tropospheric ozone is an important air pollutant with known detrimental effects for several crops. Ozone effects on seed yield, oil percentage, oil yield and 1000 seed weight were examined for spring oilseed rape ( Brassica napus cv. Ability). For broccoli ( Brassica oleracea L. cv. Italica cv. Monaco) the effects on fresh marketable weight and total dry weight were studied. Current ozone levels were compared with an increase of 20 and 40 ppb during 8 h per day, over the entire growing season. Oilseed rape seed yield was negatively correlated with ozone dose indices calculated from emergence until harvest. This resulted in an R2 of 0.24 and 0.26 ( p < 0.001) for the accumulated hourly O 3 exposure over a threshold of 40 ppb (AOT40) and the phytotoxic ozone dose above a threshold of 6 nmol m -2 s -1 (POD 6) respectively. Estimated critical levels, above which 5% yield reduction is expected, were 3.7 ppm h and 4.4 mmol m -2 respectively. Our results also confirm that a threshold value of 6 nmol s -1 m -2 projected leaf area, as recommended for agricultural crops (UNECE, Mills, 2004), can indeed be applied for spring oilseed rape. The reduction of oilseed rape yield showed the highest correlation with the ozone uptake during the vegetative growth stage: when only the first 47 days after emergence were used to calculate POD 6, R2 values increased up to 0.476 or even 0.545 when the first 23 days were excluded. The highest ozone treatments, corresponding to the future ambient level by 2100 (IPCC, Meehl et al., 2007), led to a reduction of approximately 30% in oilseed rape seed yield in comparison to the current ozone concentrations. Oil percentage was also significantly reduced in response to ozone ( p < 0.001). As a consequence oil yield was even more severely affected by elevated ozone exposure compared to seed yield: critical levels for oil yield dropped to 3.2 ppm h and 3.9 mmol m -2. For broccoli the applied ozone doses had no effect on yield.

  13. In vivo measurements of changes in pH triggered by oxalic acid in leaf tissue of transgenic oilseed rape.

    PubMed

    Zou, Qiu-Ju; Liu, Sheng-Yi; Dong, Xu-Yan; Bi, Yan-Hua; Cao, Yuan-Cheng; Xu, Qiao; Zhao, Yuan-Di; Chen, Hong

    2007-01-01

    Oxalic acid (OA), a non-host-specific toxin secreted by Sclerotinia sclerotiorum during pathogenesis, has been demonstrated to be a major phytotoxic and pathogenic factor. Oxalate oxidase (OXO) is an enzyme associated with the detoxification of OA, and hence the introduction of an OXO gene into oilseed rape (Brassica napus L.) to break down OA may be an alternative way of increasing the resistance of the plant to Sclerotinia sclerotiorum. In order to investigate the activation of OXO in transgenic oilseed rape, a convenient and accessible method was used to monitor changes in pH in response to stress induced by OA. The pH sensor, a platinum microcylinder electrode modified using polyaniline film, exhibited a linear response within the pH range from 3 to 7, with a Nernst response slope of 70 mV/pH at room temperature. The linear correlation coefficient was 0.9979. Changes induced by OA in the pH values of leaf tissue of different oilseed rape species from Brassica napus L. were monitored in real time in vivo using this electrode. The results clearly showed that the transgenic oilseed rape was more resistant to OA than non-transgenic oilseed rape. PMID:17623369

  14. Development of a biologically based fertilizer, incorporating Bacillus megaterium A6, for improved phosphorus nutrition of oilseed rape.

    PubMed

    Hu, Xiaojia; Roberts, Daniel P; Xie, Lihua; Maul, Jude E; Yu, Changbing; Li, Yinshui; Zhang, Shujie; Liao, Xing

    2013-04-01

    Sustainable methods with diminished impact on the environment need to be developed for the production of oilseed rape in China and other regions of the world. A biological fertilizer consisting of Bacillus megaterium A6 cultured on oilseed rape meal improved oilseed rape seed yield (P < 0.0001) relative to the nontreated control in 2 greenhouse pot experiments using natural soil. This treatment resulted in slightly greater yield than oilseed rape meal without strain A6 in 1 of 2 experiments, suggesting a role for strain A6 in improving yield. Strain A6 was capable of solubilizing phosphorus from rock phosphate in liquid culture and produced enzymes capable of mineralizing organic phosphorus (acid phosphatase, phytase) in liquid culture and in the biological fertilizer. The biologically based fertilizer, containing strain A6, improved plant phosphorus nutrition in greenhouse pot experiments resulting in significantly greater available phosphorus in natural soil and in significantly greater plant phosphorus content relative to the nontreated control. Seed yield and available phosphorus in natural soil were significantly greater with a synthetic chemical fertilizer treatment, reduced in phosphorus content, than the biological fertilizer treatment, but a treatment containing the biological fertilizer combined with the synthetic fertilizer provided the significantly greatest seed yield, available phosphorus in natural soil, and plant phosphorus content. These results suggest that the biological fertilizer was capable of improving oilseed rape seed yield, at least in part, through the phosphorus-solubilizing activity of B. megaterium A6. PMID:23586746

  15. Use of formulated Trichoderma sp. Tri-1 in combination with reduced rates of chemical pesticide for control of Sclerotinia sclerotiorium on oilseed rape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sustainable strategies for control of Sclerotinia sclerotiorum on oilseed rape are needed. Here we tested combinations of Trichoderma sp. Tri-1, formulated with oilseed rape seedcake and straw, with reduced application rates of the chemical pesticide Carbendazim for control of this pathogen on oils...

  16. Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides.

    PubMed

    Saji, Hikaru; Nakajima, Nobuyoshi; Aono, Mitsuko; Tamaoki, Masanori; Kubo, Akihiro; Wakiyama, Seiji; Hatase, Yoriko; Nagatsu, Masato

    2005-01-01

    An investigation was carried out to monitor the escape and spread of oilseed rape (Brassica napus) transgenic plants and the introgression of transgenes to its closely related feral species in Japan. We screened a total of about 7500 feral B. napus, 300 B. rapa, and 5800 B. juncea seedlings from maternal plants in 143 locations at several ports, roadsides, and riverbanks. The presence of glufosinate-resistance or glyphosate-resistance transgenes in these seedlings was confirmed by means of herbicide treatments and also immunochemical and DNA analyses. B. napus plants with herbicide-resistant transgenic seeds were found at five of six major ports and along two of four sampled roadsides in the Kanto District. Transgenic oilseed rape plants have not been commercially cultivated in Japan, suggesting that the transgenes would probably have come from imported transgenic seeds that were spilled during transportation to oilseed processing facilities. No transgenes were detected in seeds collected from B. napus plants growing along riverbanks in the Kanto District or in seeds from closely related species (B. rapa and B. juncea). To our knowledge, this is the first published example of feral, transgenic populations occurring in a nation where the transgenic crop has not been cultivated commercially. PMID:16827549

  17. Insect pollination enhances seed yield, quality, and market value in oilseed rape.

    PubMed

    Bommarco, Riccardo; Marini, Lorenzo; Vaissière, Bernard E

    2012-08-01

    The relationships between landscape intensification, the abundance and diversity of pollinating insects, and their contributions to crop yield, quality, and market value are poorly studied, despite observed declines in wild and domesticated pollinators. Abundance and species richness of pollinating insects were estimated in ten fields of spring oilseed rape, Brassica napus var. SW Stratos™, located along a gradient of landscape compositions ranging from simple landscapes dominated by arable land to heterogeneous landscapes with extensive cover of semi-natural habitats. In each field, we assessed the contribution of wind and insect pollination to seed yield, seed quality (individual seed weight and oil and chlorophyll contents), and market value in a block experiment with four replicates and two treatments: (1) all flowers were accessible to insects, self and wind pollination, and (2) flowers enclosed in tulle net bags (mesh: 1 × 1 mm) were accessible only to wind and self pollination. Complex landscapes enhanced the overall abundance of wild insects as well as the abundance and species richness of hoverflies. This did not translate to a higher yield, probably due to consistent pollination by honey bees across all fields. However, the pollination experiment showed that insects increased seed weight per plant by 18% and market value by 20%. Seed quality was enhanced by insect pollination, rendering heavier seeds as well as higher oil and lower chlorophyll contents, clearly showing that insect pollination is required to reach high seed yield and quality in oilseed rape. Our study demonstrates considerable and previously underestimated contributions from pollinating insects to both the yield and the market value of oilseed rape. PMID:22311256

  18. Nitrous oxide emissions from crop rotations including wheat, oilseed rape and dry peas

    NASA Astrophysics Data System (ADS)

    Jeuffroy, M. H.; Baranger, E.; Carrouée, B.; de Chezelles, E.; Gosme, M.; Hénault, C.; Schneider, A.; Cellier, P.

    2013-03-01

    Approximately 65% of anthropogenic emissions of N2O, a potent greenhouse gas (GHG), originate from soils at a global scale, and particularly after N fertilisation of the main crops in Europe. Thanks to their capacity to fix atmospheric N2 through biological fixation, legumes can reduce N fertilizer use, and possibly N2O emissions. Nevertheless, the decomposition of crop organic matter during the crop cycle and residue decomposition, and possibly the N fixation process itself, could lead to N2O emissions. The objective of this study was to quantify N2O emissions from a dry pea crop (Pisum sativum, harvested at maturity) and from the subsequent crops in comparison with N2O emissions from wheat and oilseed rape crops, fertilized or not, in various rotations. A field experiment was conducted over 4 consecutive years to compare the emissions during the pea crop, in comparison with those during the wheat (fertilized or not) or oilseed rape crops, and after the pea crop, in comparison with other preceding crops. N2O fluxes were measured using static chambers. In spite of low N2O fluxes, mainly due to the site's soil characteristics, fluxes during the crop were significantly lower for pea and unfertilized wheat than for fertilized wheat and oilseed rape. The effect of the preceding crop was not significant, while soil mineral N at harvest was higher after the pea crop. These results should be confirmed over a wider range of soil types. Nevertheless, they demonstrate the absence of N2O emissions linked to the symbiotic N fixation process, and allow us to estimate the decrease in N2O emissions by 20-25% through including one pea crop in a three-year rotation. On a larger scale, this reduction of GHG emissions at field level has to be added to the decrease due to the reduced production and transport of the N fertilizer not applied to the pea crop.

  19. EFFICACY DATA OF CYANTRANILIPROLE CONTAINING INSECTICIDE PREPARATION ON CABBAGE ROOT FLY (DELIA RADICUM LINNAEUS) IN WINTER OILSEED RAPE.

    PubMed

    Farkas, I; Molnár, I; Somlyay, I; Tóth, E

    2015-01-01

    The increasing pressure of oilseed rape pests emphasized the need to improve the insecticide portfolio, i.e. register new active ingredients with new insecticide mode of action. The tested seed treatment formulation applied at 32; 40 and 50 UAT rate of containing cyantraniliprole as active substance. 40 UAT rate gives acceptable control of the Cabbage root fly in each trial. Despite the long lasting flight and egg laying period of cabbage root fly, the standard control products and also this product give 50-65% efficacy. It is enough to reduce damage of the Cabbage root fly and prevent economical damage in oilseed rape. PMID:27145579

  20. Ozone effects on yield quality of spring oilseed rape and broccoli

    NASA Astrophysics Data System (ADS)

    Vandermeiren, Karine; De Bock, Maarten; Horemans, Nele; Guisez, Yves; Ceulemans, Reinhart; De Temmerman, Ludwig

    2012-02-01

    The impact of elevated tropospheric ozone (O 3) on the quality of spring oilseed rape ( Brassica napus cv Ability) and broccoli ( Brassica oleracea L. cv Italic cv Monaco) was assessed during a three year Open - Top Chamber (OTC) experiment. Current ambient O 3 levels were compared to an increase of 20 and 40 ppb during 8 h per day over the entire growing season. The qualitative responses were expressed as a function of the accumulated hourly O 3 concentrations over a threshold of 40 ppb (AOT40) and the phytotoxic O 3 dose above a threshold of 6 nmol s -1 m -2 projected leaf area (POD 6). Our results provide clear evidence that O 3 has an influence on the qualitative attributes of the harvested products of these Brassica species. The responses were comparable whether they were expressed as a function of the accumulated O 3 concentrations or of the modelled O 3 uptake. The protein concentration of oilseed rape seeds and broccoli heads was significantly increased in response to O 3. There was also a shift in the fatty acid composition of the vegetable oil derived from seeds of oilseed rape. Oleic acid (18:1) declined significantly ( p < 0.05) in favour of linoleic acid (18:2) ( p < 0.01). There was no change in the relative proportion of linolenic acid (18:3). The suppression of monounsaturated fatty acids ( p < 0.05) coincided with a positive response of the % saturated fatty acids ( p < 0.05). In rapeseed oil the observed decrease in vitamin E content was due to a reduction of γ-tocopherol (TOC, p < 0.001). α-TOC, the most active form of vitamin E in humans, was not influenced by O 3. There was no change in the glucosinolate (GSL) content of oilseed rape seeds. In broccoli an important shift occurred from indolic to aliphatic GSLs although the total GSL concentration was not changed. The increase in the aliphatic/indolic GSL ratio ( p < 0.001) may be important in relation to the anticarcinogenic properties of these vegetables. The vitamin C (ascorbate - ASC) and

  1. [Rapid detection of nitrogen content and distribution in oilseed rape leaves based on hyperspectral imaging].

    PubMed

    Zhang, Xiao-Lei; Liu, Fei; Nie, Peng-Cheng; He, Yong; Bao, Yi-Dan

    2014-09-01

    Visible and near infrared (Vis-NIR) hyperspectral imaging system was carried out to rapidly determinate the content and estimate the distribution of nitrogen (N) in oilseed rape leaves. Hyperspectral images of 420 leaf samples were acquired at seedling, flowering and pod stages. The spectral data of rape leaves were extracted from the region of interest (ROI) in the wave- length range of 380-1,030 nm. Different spectra preprocessing including Savitzky-Golay smoothing (SG), standard normal variate (SNV), multiplicative scatter correction (MSC), first and second derivatives were applied to improve the signal to noise ratio. Among 471 wavelengths, only twelve wavelengths (467, 557, 665, 686, 706, 752, 874, 879, 886, 900, 978 and 995 nm) were selected by successive projections algorithm(SPA) as the effective wavelengths for N prediction. Based on these effective wavelengths, partial least squares(PLS) and least-squares support vector machines (LS-SVM) calibration models were established for the determination of N content. Reasonable estimation accuracy was obtained, with Rp of 0.807 and RMSEP of 0.387 by PLS and Rp of 0.836 and RMSEP of 0.358 by LS-SVM, respectively. Considering the simple structure and satisfying results of PLS model, SPA-PLS model was used to generate the distribution maps of N content in rape leaves. The concentrations of N were calculated at each pixel of hyperspectral images at the selected effective wavelengths by inputting its correspond- ing spectrum into the established SPA-PLS model. Different colour represented the change in N content in the rape leaves under different fertilizer treatments. By including all pixels within the selected ROI, the average N status can be displayed in more detail and visualised. The visualization of N distribution could be helpful to understanding the change in N content in rape leaves during rape growth period and facilitate discovering the difference of N content within one sample as well as among the samples

  2. Cadmium stress alters the redox reaction and hormone balance in oilseed rape (Brassica napus L.) leaves.

    PubMed

    Yan, Hui; Filardo, Fiona; Hu, Xiaotao; Zhao, Xiaomin; Fu, DongHui

    2016-02-01

    In order to understand the physiological response of oilseed rape (Brassica napus L.) leaves to cadmium (Cd) stress and exploit the physiological mechanisms involved in Cd tolerance, macro-mineral and chlorophyll concentrations, reactive oxygen species (ROS) accumulation, activities of enzymatic antioxidants, nonenzymatic compounds metabolism, endogenous hormonal changes, and balance in leaves of oilseed rape exposed to 0, 100, or 200 μM CdSO4 were investigated. The results showed that under Cd exposure, Cd concentrations in the leaves continually increased while macro-minerals and chlorophyll concentrations decreased significantly. Meanwhile, with increased Cd stress, superoxide anion (O2(• -)) production rate and hydrogen peroxide (H2O2) concentrations in the leaves increased significantly, which caused malondialdehyde (MDA) accumulation and oxidative stress. For scavenging excess accumulated ROS and alleviating oxidative injury in the leaves, the activity of enzymatic antioxidants, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), was increased significantly at certain stress levels. However, with increased Cd stress, the antioxidant enzyme activities all showed a trend towards reduction. The nonenzymatic antioxidative compounds, such as proline and total soluble sugars, accumulated continuously with increased Cd stress to play a long-term role in scavenging ROS. In addition, ABA levels also increased continuously with Cd stress while ZR decreased and the ABA/ZR ratio increased, which might also be providing a protective role against Cd toxicity. PMID:26498815

  3. Fixed-route monitoring and a comparative study of the occurrence of herbicide-resistant oilseed rape (Brassica napus L.) along a Japanese roadside.

    PubMed

    Nishizawa, Toru; Nakajima, Nobuyoshi; Tamaoki, Masanori; Aono, Mitsuko; Kubo, Akihiro; Saji, Hikaru

    2016-01-01

    Previously, we conducted a roadside survey to reveal the occurrence of genetically modified (GM) oilseed rape along a Japanese roadside (Route 51). In this study, we performed successive and thorough fixed-route monitoring in 5 sections along another road (Route 23). Oilseed rape plants were detected on both sides of the road in each section between autumn 2009 and winter 2013, which included 3 flowering seasons. In four sections, more plants were found on the side of the road leading from the Yokkaichi port than on the opposite side. In the fifth section, the presence of clogged drains on the roadside, where juvenile plants concentrated, caused the opposite distribution: oilseed rape predominantly occurred along the inbound lanes (leading to the Yokkaichi port) in 2010 and 2012. Unlike in our previous survey, glyphosate- or glufosinate-resistant oilseed rape plants were abundant (>75% of analyzed plants over 3 years). Moreover, a few individuals bearing both herbicide resistance traits were also detected in some sections. The spillage of imported seeds may explain the occurrence of oilseed rape on the roadside. The abundance of herbicide-resistant oilseed rape plants may reflect the extent of contamination with GM oilseed rape seed within imports. PMID:26838503

  4. Measurement of aspartic acid in oilseed rape leaves under herbicide stress using near infrared spectroscopy and chemometrics.

    PubMed

    Zhang, Chu; Kong, Wenwen; Liu, Fei; He, Yong

    2016-01-01

    Oilseed rape is used as both food and a renewable energy resource. Physiological parameters, such as the amino acid aspartic acid, can indicate the growth status of oilseed rape. Traditional detection methods are laborious, time consuming, costly, and not usable in the field. Here, we investigate near infrared spectroscopy (NIRS) as a fast and non-destructive detection method of aspartic acid in oilseed rape leaves under herbicide stress. Different spectral pre-processing methods were compared for optimal prediction performance. The variable selection methods were applied for relevant variable selection, including successive projections algorithm (SPA), Monte Carlo-uninformative variable elimination (MC-UVE) and random frog (RF). The selected effective wavelengths (EWs) were used as input by multiple linear regression (MLR), partial least squares (PLS) and least-square support vector machine (LS-SVM). The best predictive performance was achieved by SPA-LS-SVM (Raw) model using 22 EWs, and the prediction results were Rp = 0.9962 and RMSEP = 0.0339 for the prediction set. The result indicated that NIR combined with LS-SVM is a powerful new method to detect aspartic acid in oilseed rape leaves under herbicide stress. PMID:27441244

  5. BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape.

    PubMed

    Chen, Quan; Wang, Jie; Hou, Mingsheng; Liu, Shengyi; Huang, Junyan; Cai, Li

    2015-08-01

    Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS3-silenced (BnSGS3-Si) oilseed rape (cv. Zhongshuang No. 6) plants. These three viruses are Oilseed rape mosaic virus (ORMV), Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation) for breeding virus-resistant oilseed rape varieties. PMID:26225990

  6. Development of crop-specific transposable element (SINE) markers for studying gene flow from oilseed rape to wild radish.

    PubMed

    Prieto, J L; Pouilly, N; Jenczewski, E; Deragon, J M; Chèvre, A M

    2005-08-01

    The screening of wild populations for evidence of gene flow from a crop to a wild related species requires the unambiguous detection of crop genes within the genome of the wild species, taking into account the intraspecific variability of each species. If the crop and wild relatives share a common ancestor, as is the case for the Brassica crops and their wild relatives (subtribe Brassiceae), the species-specific markers needed to make this unambiguous detection are difficult to identify. In the model oilseed rape (Brassica napus, AACC, 2n = 38)-wild radish (Raphanus raphanistrum, RrRr, 2n = 18) system, we utilized the presence or absence of a short-interspersed element (SINE) at a given locus to develop oilseed rape-specific markers, as SINE insertions are irreversible. By means of sequence-specific amplified polymorphism (SINE-SSAP) reactions, we identified and cloned 67 bands specific to the oilseed rape genome and absent from that of wild radish. Forty-seven PCR-specific markers were developed from three combinations of primers anchored either in (1) the 5'- and 3'-genomic sequences flanking the SINE, (2) the 5'-flanking and SINE internal sequences or (3) the SINE internal and flanking 3'-sequences. Seventeen markers were monomorphic whatever the oilseed rape varieties tested, whereas 30 revealed polymorphism and behaved either as dominant (17) or co-dominant (13) markers. Polymorphic markers were mapped on 19 genomic regions assigned to ten linkage groups. The markers developed will be efficient tools to trace the occurrence and frequency of introgressions of oilseed rape genomic region within wild radish populations. PMID:15942756

  7. BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape

    PubMed Central

    Chen, Quan; Wang, Jie; Hou, Mingsheng; Liu, Shengyi; Huang, Junyan; Cai, Li

    2015-01-01

    Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov) and BnSGS3-silenced (BnSGS3-Si) oilseed rape (cv. Zhongshuang No. 6) plants. These three viruses are Oilseed rape mosaic virus (ORMV), Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation) for breeding virus-resistant oilseed rape varieties. PMID:26225990

  8. Pollen-mediated intraspecific gene flow from herbicide resistant oilseed rape (Brassica napus L.).

    PubMed

    Hüsken, Alexandra; Dietz-Pfeilstetter, Antje

    2007-10-01

    The cultivation of genetically modified (GM) herbicide resistant oilseed rape (Brassica napus) has increased over the past few years. The transfer of herbicide resistance genes via pollen (gene flow) from GM crops to non-GM crops is of relevance for the realisation of co-existence of different agricultural cultivation forms as well as for weed management. Therefore the likelihood of pollen-mediated gene flow has been investigated in numerous studies. Despite the difficulty to compare different experiments with varying levels of outcrossing, we performed a literature search for world-wide studies on cross-fertilisation in fully fertile oilseed rape. The occurrence and frequency of pollen-mediated intraspecific gene flow (outcrossing rate) can vary according to cultivar, experimental design, local topography and environmental conditions. The outcrossing rate from one field to another depends also on the size and arrangement of donor and recipient populations and on the ratio between donor and recipient plot size. The outcrossing levels specified in the presented studies are derived mostly from experiments where the recipient field is either surrounding the donor field (continuous design) or is located as a patch at different distances from the donor field (discontinuous design). Reports of gene flow in Brassica napus generally show that the amount of cross-fertilisation decreases as the distance from the pollen source increases. The evidence given in various studies reveals that the bulk of GM cross-fertilisation occurs within the first 10 m of the recipient field. The removal of the first 10 m of a non-transgenic field facing a GM crop might therefore be more efficient for reducing the total level of cross-fertilisation in a recipient sink population than to recommend separation distances. Future experiments should investigate cross-fertilisation with multiple adjacent donor fields at the landscape level under different spatial distributions of rapeseed cultivars

  9. Application of a rotation system to oilseed rape and rice fields in Cd-contaminated agricultural land to ensure food safety.

    PubMed

    Yu, Lingling; Zhu, Junyan; Huang, Qingqing; Su, Dechun; Jiang, Rongfeng; Li, Huafen

    2014-10-01

    This field experiment analyzed the phytoremediation effects of oilseed rape in moderately cadmium (Cd)-contaminated farmland and the food safety of successive rice in an oilseed rape-rice rotation system. Two oilseed rape cultivars accumulated Cd at different rates. The rapeseed cultivar Zhucang Huazi exhibited high Cd accumulation rates, higher than the legal limit for human consumption (0.2mgkg(-1)); Cd concentrations in the cultivar Chuanyou II-93 were all below the maximum allowed level. Planting oilseed rape increased the uptake of Cd by the successive rice crop compared with a previous fallow treatment. Most Cd concentrations of brown rice were below the maximum allowed level. The phytoextraction efficiency was lower in the moderately Cd-contaminated soil in field experiments. The results suggest screening rice cultivars with lower Cd accumulation can assure the food safety; the mobilization of heavy metals by roots of different plant species should be considered during crop rotation to assure food safety. PMID:25108175

  10. The presence of Bt-transgenic oilseed rape in wild mustard populations affects plant growth.

    PubMed

    Liu, Yongbo; Stewart, C Neal; Li, Junsheng; Huang, Hai; Zhang, Xitao

    2015-12-01

    The adventitious presence of transgenic plants in wild plant populations is of ecological and regulatory concern, but the consequences of adventitious presence are not well understood. Here, we introduced Bacillus thuringiensis Cry1Ac (Bt)-transgenic oilseed rape (Bt OSR, Brassica napus) with various frequencies into wild mustard (Brassica juncea) populations. We sought to better understand the adventitious presence of this transgenic insecticidal crop in a wild-relative plant population. We assessed the factors of competition, resource availability and diamondback moth (Plutella xylostella) infestation on plant population dynamics. As expected, Bt OSR performed better than wild mustard in mixed populations under herbivore attack in habitats with enough resources, whereas wild mustard had higher fitness when Bt OSR was rarer in habitats with limited resources. Results suggest that the presence of insect-resistant transgenic plants could decrease the growth of wild mustard and Bt OSR plants and their populations, especially under high herbivore pressure. PMID:26338267

  11. Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture1[OPEN

    PubMed Central

    Schwender, Jörg; Hebbelmann, Inga; Heinzel, Nicolas; Hildebrandt, Tatjana; Rogers, Alistair; Klapperstück, Matthias; Schreiber, Falk; Borisjuk, Ljudmilla; Rolletschek, Hardy

    2015-01-01

    Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. Overall, we observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Quantitative data were also used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 3′,5′-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism. PMID:25944824

  12. Thiamethoxam: Assessing flight activity of honeybees foraging on treated oilseed rape using radio frequency identification technology.

    PubMed

    Thompson, Helen; Coulson, Mike; Ruddle, Natalie; Wilkins, Selwyn; Harkin, Sarah

    2016-02-01

    The present study was designed to assess homing behavior of bees foraging on winter oilseed rape grown from seed treated with thiamethoxam (as Cruiser OSR), with 1 field drilled with thiamethoxam-treated seed and 2 control fields drilled with fungicide-only-treated seed. Twelve honeybee colonies were used per treatment group, 4 each located at the field edge (on-field site), at approximately 500 m and 1000 m from the field. A total of nearly 300 newly emerged bees per colony were fitted (tagged) with Mic3 radio frequency identification (RFID) transponders and introduced into each of the 36 study hives. The RFID readers fitted to the entrances of the test colonies were used to monitor the activity of the tagged bees for the duration of the 5-wk flowering period of the crop. These activity data were analyzed to assess any impact on flight activity of bees foraging on the treated compared with untreated crops. Honeybees were seen to be actively foraging within all 3 treatment groups during the exposure period. The data for the more than 3000 RFID-tagged bees and more than 90 000 foraging flights monitored throughout the exposure phase for the study follow the same trends across the treatment and controls and at each of the 3 apiary distances, indicating that there were no effects from foraging on the treated crop. Under the experimental conditions, there was no effect of foraging on thiamethoxam-treated oilseed rape on honeybee flight activity or on their ability to return to the hive. PMID:26222207

  13. Modelling gene flow between oilseed rape and wild radish. I. Evolution of chromosome structure.

    PubMed

    Chèvre, A M; Adamczyk, K; Eber, F; Huteau, V; Coriton, O; Letanneur, J C; Laredo, C; Jenczewski, E; Monod, H

    2007-01-01

    The assessment of gene flow from crop species to weeds has found a new emphasis over the last years because of the marketing of transgenic crops and the possible selective advantage that crop (trans)gene may confer to the weeds. Several studies focused on the F1 interspecific hybrid production but few data are available on the factors affecting the genetic structure of advanced generations. It depends on the genomic structure of the species concerned as well as on the degree of their genome homology that affect the occurrence of intergenomic recombination. Oilseed rape (Brassica napus, AACC, 2n = 38)-wild radish (Raphanus raphanistrum, RrRr, 2n = 18), a distantly related weed, is a good model to address such questions. From seven male sterile oilseed rape lines carrying an herbicide tolerance transgene, F1 interspecific hybrids and four advanced generations were produced under field conditions with wild radish as pollinator. Observation of hybrid chromosome numbers across four generations revealed a high variability, especially in the "BC1" generation. A regression model was fitted in order to describe the relationship between parent and offspring chromosome numbers. The effects of generation, transgenic line and selection pressure on the mean relationship were investigated. The first two factors had an influence on the rate of decrease of chromosome numbers, whereas selection pressure resulted in the presence of an additional chromosome in the herbicide treated plants. The model provided a convenient framework for analysing how chromosome numbers evolve over successive hybridization events and it may prove useful as a basis for simulation-based approaches. PMID:17091264

  14. Glucosinolate Biosynthesis (Further Characterization of the Aldoxime-Forming Microsomal Monooxygenases in Oilseed Rape Leaves).

    PubMed Central

    Bennett, R. N.; Hick, A. J.; Dawson, G. W.; Wallsgrove, R. M.

    1995-01-01

    The initial steps in glucosinolate biosynthesis are thought to proceed from amino acids, via N-hydroxy amino acids, to aldoximes. We showed previously that microsomes from green leaves of oilseed rape (Brassica napus cv Bienvenu) contain two distinct monooxygenases that catalyze the conversion of homophenylalanine and dihomomethionine to their respective aldoximes. Further characterization of these enzymes has now demonstrated that the latter enzyme catalyzes the NADPH-dependent oxidative decarboxylation of two higher homologs of methionine, in addition to dihomomethionine. No activity was found for either enzyme with L-methionine, DL-homomethionine, L-phenylalanine, L-tyrosine, or L-tryptophan. Both of these rape monooxygenase activities are dependent on O2, not requiring any other O2 species or radical. The presence of an unoxidized sulfur atom and its relative position in the side chain of the aliphatic substrates are important for binding to the active site of the methionine-homolog enzyme. Neither enzyme has any characteristics of a cytochrome P450-type enzyme, and antiserum raised against cytochrome P450 reductase did not significantly inhibit monooxygenase activity. PMID:12228596

  15. Nitrogen availability impacts oilseed rape (Brassica napus L.) plant water status and proline production efficiency under water-limited conditions.

    PubMed

    Albert, Benjamin; Le Cahérec, Françoise; Niogret, Marie-Françoise; Faes, Pascal; Avice, Jean-Christophe; Leport, Laurent; Bouchereau, Alain

    2012-08-01

    Large amounts of nitrogen (N) fertilizers are used in the production of oilseed rape. However, as low-input methods of crop management are introduced crops will need to withstand temporary N deficiency. In temperate areas, oilseed rape will also be affected by frequent drought periods. Here we evaluated the physiological and metabolic impact of nitrate limitation on the oilseed rape response to water deprivation. Different amounts of N fertilizer were applied to plants at the vegetative stage, which were then deprived of water and rehydrated. Both water and N depletion accelerated leaf senescence and reduced leaf development. N-deprived plants exhibited less pronounced symptoms of wilting during drought, probably because leaves were smaller and stomata were partially closed. Efficiency of proline production, a major stress-induced diversion of nitrogen metabolism, was assessed at different positions along the whole plant axis and related to leaf developmental stage and water status indices. Proline accumulation, preferentially in younger leaves, accounted for 25-85% of the free amino acid pool. This was mainly due to a better capacity for proline synthesis in fully N-supplied plants whether they were subjected to drought or not, as deduced from the expression patterns of the proline metabolism BnP5CS and BnPDH genes. Although less proline accumulated in the oldest leaves, a significant amount was transported from senescing to emerging leaves. Moreover, during rehydration proline was readily recycled. Our results therefore suggest that proline plays a significant role in leaf N remobilization and in N use efficiency in oilseed rape. PMID:22526495

  16. Potential gene flow of two herbicide-tolerant transgenes from oilseed rape to wild B. juncea var. gracilis.

    PubMed

    Song, Xiaoling; Wang, Zhou; Zuo, Jiao; Huangfu, Chaohe; Qiang, Sheng

    2010-05-01

    Four successive reciprocal backcrosses between F(1) (obtained from wild Brassica juncea as maternal plants and transgenic glyphosate- or glufosinate-tolerant oilseed rape, B. napus, as paternal plants) or subsequent herbicide-tolerant backcross progenies and wild B. juncea were achieved by hand pollination to assess potential transgene flow. The third and forth reciprocal backcrosses produced a number of seeds per silique similar to that of self-pollinated wild B. juncea, except in plants with glufosinate-tolerant backcross progeny used as maternal plants and wild B. juncea as paternal plants, which produced fewer seeds per silique than did self-pollinated wild B. juncea. Germination percentages of reciprocal backcross progenies were high and equivalent to those of wild B. juncea. The herbicide-tolerant first reciprocal backcross progenies produced fewer siliques per plant than did wild B. juncea, but the herbicide-tolerant second or third reciprocal backcross progenies did not differ from the wild B. juncea in siliques per plant. The herbicide-tolerant second and third reciprocal backcross progenies produced an amount of seeds per silique similar to that of wild B. juncea except for with the glufosinate-tolerant first and second backcross progeny used as maternal plants and wild B. juncea as paternal plants. In the presence of herbicide selection pressure, inheritance of the glyphosate-tolerant transgene was stable across the second and third backcross generation, whereas the glufosinate-tolerant transgene was maintained, despite a lack of stabilized introgression. The occurrence of fertile, transgenic weed-like plants after only three crosses (F(1), first backcross, second backcross) suggests a potential rapid spread of transgenes from oilseed rape into its wild relative wild B. juncea. Transgene flow from glyphosate-tolerant oilseed rape might be easier than that from glufosinate-tolerant oilseed rape to wild B. juncea. The original insertion site of the

  17. Detection of Glutamic Acid in Oilseed Rape Leaves Using Near Infrared Spectroscopy and the Least Squares-Support Vector Machine

    PubMed Central

    Bao, Yidan; Kong, Wenwen; Liu, Fei; Qiu, Zhengjun; He, Yong

    2012-01-01

    Amino acids are quite important indices to indicate the growth status of oilseed rape under herbicide stress. Near infrared (NIR) spectroscopy combined with chemometrics was applied for fast determination of glutamic acid in oilseed rape leaves. The optimal spectral preprocessing method was obtained after comparing Savitzky-Golay smoothing, standard normal variate, multiplicative scatter correction, first and second derivatives, detrending and direct orthogonal signal correction. Linear and nonlinear calibration methods were developed, including partial least squares (PLS) and least squares-support vector machine (LS-SVM). The most effective wavelengths (EWs) were determined by the successive projections algorithm (SPA), and these wavelengths were used as the inputs of PLS and LS-SVM model. The best prediction results were achieved by SPA-LS-SVM (Raw) model with correlation coefficient r = 0.9943 and root mean squares error of prediction (RMSEP) = 0.0569 for prediction set. These results indicated that NIR spectroscopy combined with SPA-LS-SVM was feasible for the fast and effective detection of glutamic acid in oilseed rape leaves. The selected EWs could be used to develop spectral sensors, and the important and basic amino acid data were helpful to study the function mechanism of herbicide. PMID:23203052

  18. [Prediction of SPAD value in oilseed rape leaves using hyperspectral imaging technique].

    PubMed

    Ding, Xi-bin; Liu, Fei; Zhang, Chu; He, Yong

    2015-02-01

    In the present work, prediction models of SPAD value (Soil and Plant Analyzer Development, often used as a parameter to indicate chlorophyll content) in oilseed rape leaves were successfully built using hyperspectral imaging technique. The hy perspectral images of 160 oilseed rape leaf samples in the spectral range of 380-1030 nm were acquired. Average spectrum was extracted from the region of interest (ROI) of each sample. We chose spectral data in the spectral range of 500-900 nm for analysis. Using Monte Carlo partial least squares(MC-PLS) algorithm, 13 samples were identified as outliers and eliminated. Based on the spectral information and measured SPAD values of the rest 147 samples, several estimation models have been built based on different parameters using different algorithms for comparison, including: (1) a SPAD value estimation model based on partial least squares(PLS) in the whole wavelength region of 500-900 nm; (2) a SPAD value estimation model based on successive projections algorithmcombined with PLS(SPA-PLS); (3) 4 kind of simple experience SPAD value estimation models in which red edge position was used as an argument; (4) 4 kind of simple experience SPAD value estimation models in which three vegetation indexes R710/R760, (R750-R705)/(R750-R705) and R860/(R550 x R708), which all have been proved to have a good relevance with chlorophyll content, were used as an argument respectively; (5) a SPAD value estimation model based on PLS using the 3 vegetation indexes mentioned above. The results indicate that the optimal prediction performance is achieved by PLS model in the whole wavelength region of 500-900 nm, which has a correlation coefficient(r(p)) of 0.8339 and a root mean squares error of predicted (RMSEP) of 1.52. The SPA-PLS model can provide avery close prediction result while the calibration computation has been significantly reduced and the calibration speed has been accelerated sharply. For simple experience models based on red edge

  19. Influence of development stage of spring oilseed rape and spring wheat on interception of wet-deposited radiocaesium and radiostrontium

    NASA Astrophysics Data System (ADS)

    Bengtsson, S. B.; Eriksson, J.; Gärdenäs, A. I.; Rosén, K.

    2012-12-01

    The dry and wet deposition of radionuclides released into the atmosphere can be intercepted by vegetation in terrestrial ecosystems. The aim of this study was to quantify the interception of wet deposited 134Cs and 85Sr by spring oilseed rape (Brassíca napus L.) and spring wheat (Tríticum aestívum L.). The dependency of the intercepted fraction (f) on total above ground plant biomass, growing stage and the Leaf Area Index (LAI) was quantified. A trial was established in Uppsala (east central Sweden), with land management in accordance to common agricultural practices. The field trial was a randomised block design of 1 × 1 m2 parcels with three replicates. During the growing season of 2010, a rainfall simulator deposited 134Cs and 85Sr during six different growth stages. Two to 3 h after deposition, the biomass of the centre 25 × 25 cm2 area of each parcel was sampled and above ground biomass and LAI were measured. The radioactivity concentration and radioactivity of samples were measured by High Purity Germanium (HPGe)-detectors. For 134Cs, there was a correlation between f and LAI (r2 = 0.55, p < 0.05) for spring wheat, but not for spring oilseed rape (r2 = 0.28, p > 0.05). For 85Sr, there was a correlation between f and LAI for both crops (r2 = 0.41, p < 0.05 for spring oilseed rape and r2 = 0.48 p, <0.05 for spring wheat). There was no correlation between f and above ground plant biomass in spring oilseed rape for either 134Cs (r2 = 0.01, p > 0.05) or for 85Sr (r2 = 0.11, p > 0.05). For spring wheat, there was a correlation for both 134Cs (r2 = 0.36, p < 0.05) and 85Sr (r2 = 0.32, p < 0.05). For spring oilseed rape, f was highest at growth stage 'stem elongation' for 134Cs (0.32 ± 0.22) and 85Sr (0.41 ± 0.29). For spring wheat, f was highest at growth stage 'ripening' for both radionuclides (134Cs was 0.36 ± 0.14 and 85Sr was 0.48 ± 0.18). Thus, LAI can be used to quantify interception of both radionuclides for both crops, whereas, above ground plant

  20. Detection of feral GT73 transgenic oilseed rape (Brassica napus) along railway lines on entry routes to oilseed factories in Switzerland.

    PubMed

    Hecht, Mirco; Oehen, Bernadette; Schulze, Jürg; Brodmann, Peter; Bagutti, Claudia

    2014-01-01

    To obtain a reference status prior to cultivation of genetically modified oilseed rape (OSR, Brassica napus L.) in Switzerland, the occurrence of feral OSR was monitored along transportation routes and at processing sites. The focus was set on the detection of (transgenic) OSR along railway lines from the Swiss borders with Italy and France to the respective oilseed processing factories in Southern and Northern Switzerland (Ticino and region of Basel). A monitoring concept was developed to identify sites of largest risk of escape of genetically modified plants into the environment in Switzerland. Transport spillage of OSR seeds from railway goods cars particularly at risk hot spots such as switch yards and (un)loading points but also incidental and continuous spillage were considered. All OSR plants, including their hybridization partners which were collected at the respective monitoring sites were analyzed for the presence of transgenes by real-time PCR. On sampling lengths each of 4.2 and 5.7 km, respectively, 461 and 1,574 plants were sampled in Ticino and the region of Basel. OSR plants were found most frequently along the routes to the oilseed facilities, and in larger amounts on risk hot spots compared to sites of random sampling. At three locations in both monitored regions, transgenic B. napus line GT73 carrying the glyphosate resistance transgenes gox and CP4 epsps were detected (Ticino, 22 plants; in the region of Basel, 159). PMID:23917737

  1. Developmental Regulation of a Plasma Membrane Arabinogalactan Protein Epitope in Oilseed Rape Flowers.

    PubMed Central

    Pennell, RI; Janniche, L; Kjellbom, P; Scofield, GN; Peart, JM; Roberts, K

    1991-01-01

    We have identified and characterized the temporal and spatial regulation of a plasma membrane arabinogalactan protein epitope during development of the aerial parts of oilseed rape using the monoclonal antibody JIM8. The JIM8 epitope is expressed by the first cells of the embryo and by certain cells in the sexual organs of flowers. During embryogenesis, the JIM8 epitope ceases to be expressed by the embryo proper but is still found in the suspensor. During differentiation of the stamens and carpels, expression of the JIM8 epitope progresses from one cell type to another, ultimately specifying the endothecium and sperm cells, the nucellar epidermis, synergid cells, and the egg cell. This complex temporal sequence demonstrates rapid turnover of the JIM8 epitope. There is no direct evidence for any cell-inductive process in plant development. However, if cell-cell interactions exist in plants and participate in flower development, the JIM8 epitope may be a marker for one set of them. PMID:12324592

  2. Evidence for pollinator cost and farming benefits of neonicotinoid seed coatings on oilseed rape

    NASA Astrophysics Data System (ADS)

    Budge, G. E.; Garthwaite, D.; Crowe, A.; Boatman, N. D.; Delaplane, K. S.; Brown, M. A.; Thygesen, H. H.; Pietravalle, S.

    2015-08-01

    Chronic exposure to neonicotinoid insecticides has been linked to reduced survival of pollinating insects at both the individual and colony level, but so far only experimentally. Analyses of large-scale datasets to investigate the real-world links between the use of neonicotinoids and pollinator mortality are lacking. Moreover, the impacts of neonicotinoid seed coatings in reducing subsequent applications of foliar insecticide sprays and increasing crop yield are not known, despite the supposed benefits of this practice driving widespread use. Here, we combine large-scale pesticide usage and yield observations from oilseed rape with those detailing honey bee colony losses over an 11 year period, and reveal a correlation between honey bee colony losses and national-scale imidacloprid (a neonicotinoid) usage patterns across England and Wales. We also provide the first evidence that farmers who use neonicotinoid seed coatings reduce the number of subsequent applications of foliar insecticide sprays and may derive an economic return. Our results inform the societal discussion on the pollinator costs and farming benefits of prophylactic neonicotinoid usage on a mass flowering crop.

  3. Application of glutathione to roots selectively inhibits cadmium transport from roots to shoots in oilseed rape

    PubMed Central

    Nakamura, Shin-ichi

    2013-01-01

    Glutathione is a tripeptide involved in various aspects of plant metabolism. This study investigated the effects of the reduced form of glutathione (GSH) applied to specific organs (source leaves, sink leaves, and roots) on cadmium (Cd) distribution and behaviour in the roots of oilseed rape plants (Brassica napus) cultured hydroponically. The translocation ratio of Cd from roots to shoots was significantly lower in plants that had root treatment of GSH than in control plants. GSH applied to roots reduced the Cd concentration in the symplast sap of root cells and inhibited root-to-shoot Cd translocation via xylem vessels significantly. GSH applied to roots also activated Cd efflux from root cells to the hydroponic solution. Inhibition of root-to-shoot translocation of Cd was visualized, and the activation of Cd efflux from root cells was also shown by using a positron-emitting tracer imaging system (PETIS). This study investigated a similar inhibitory effect on root-to-shoot translocation of Cd by the oxidized form of glutathione, GSSG. Inhibition of Cd accumulation by GSH was abolished by a low-temperature treatment. Root cells of plants exposed to GSH in the root zone had less Cd available for xylem loading by actively excluding Cd from the roots. Consequently, root-to-shoot translocation of Cd was suppressed and Cd accumulation in the shoot decreased. PMID:23364937

  4. Evidence for pollinator cost and farming benefits of neonicotinoid seed coatings on oilseed rape.

    PubMed

    Budge, G E; Garthwaite, D; Crowe, A; Boatman, N D; Delaplane, K S; Brown, M A; Thygesen, H H; Pietravalle, S

    2015-01-01

    Chronic exposure to neonicotinoid insecticides has been linked to reduced survival of pollinating insects at both the individual and colony level, but so far only experimentally. Analyses of large-scale datasets to investigate the real-world links between the use of neonicotinoids and pollinator mortality are lacking. Moreover, the impacts of neonicotinoid seed coatings in reducing subsequent applications of foliar insecticide sprays and increasing crop yield are not known, despite the supposed benefits of this practice driving widespread use. Here, we combine large-scale pesticide usage and yield observations from oilseed rape with those detailing honey bee colony losses over an 11 year period, and reveal a correlation between honey bee colony losses and national-scale imidacloprid (a neonicotinoid) usage patterns across England and Wales. We also provide the first evidence that farmers who use neonicotinoid seed coatings reduce the number of subsequent applications of foliar insecticide sprays and may derive an economic return. Our results inform the societal discussion on the pollinator costs and farming benefits of prophylactic neonicotinoid usage on a mass flowering crop. PMID:26270806

  5. Sources of uncertainty in the quantification of genetically modified oilseed rape contamination in seed lots.

    PubMed

    Begg, Graham S; Cullen, Danny W; Iannetta, Pietro P M; Squire, Geoff R

    2007-02-01

    Testing of seed and grain lots is essential in the enforcement of GM labelling legislation and needs reliable procedures for which associated errors have been identified and minimised. In this paper we consider the testing of oilseed rape seed lots obtained from the harvest of a non-GM crop known to be contaminated by volunteer plants from a GM herbicide tolerant variety. The objective was to identify and quantify the error associated with the testing of these lots from the initial sampling to completion of the real-time PCR assay with which the level of GM contamination was quantified. The results showed that, under the controlled conditions of a single laboratory, the error associated with the real-time PCR assay to be negligible in comparison with sampling error, which was exacerbated by heterogeneity in the distribution of GM seeds, most notably at a small scale, i.e. 25 cm3. Sampling error was reduced by one to two thirds on the application of appropriate homogenisation procedures. PMID:17106767

  6. Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape.

    PubMed

    Huang, X Q; He, R Q; Liao, X Y; Zhou, B; Peng, W S; Lin, J Z; Tang, D Y; Zhu, Y H; Zhao, X Y; Liu, X M

    2014-01-01

    Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed rape (Brassica napus L.) were treated with GA3 at 3 stages of seed filling. We confirmed that GA3 regulates the deposition of storage reserves in developing seeds. The percentage of crude fat in the seeds increased during the early stage, but remained stable during the middle and late stages. In comparison, the percentage of total protein decreased during the early and middle stages, but significantly increased during the late stage. In addition, Q-PCR was employed to analyze the expression level of related genes in response to GA3. It was found that the expression of WRI and ABI3 transcription factors corresponded to crude fat content and total protein content, respectively. The expression of storage reserve related genes DGAT, MCAT, SUC2, and GPT was consistent with crude fat content, whereas the expression of Napin corresponded to total protein content. The results of this study indicate that exogenous GA3 has a different effect on storage reserve deposition in seed during different stages of seed filling, and the effect might be achieved via changing the expression of related genes. PMID:24535906

  7. Does pea lectin expressed transgenically in oilseed rape (Brassica napus) influence honey bee (Apis mellifera) larvae?

    PubMed

    Lehrman, Anna

    2007-01-01

    The European honey bee (Apis mellifera) is important both for pollination and for honey production. Pollen is the major protein source for bees, which exposes them directly to changes in pollen quality e.g. through genetic engineering. In order to create a worst case scenario regarding pea lectin (PSL) expressed transgenically in oilseed rape anthers and pollen, the maximum amount of dried pollen that could be mixed in an artificial diet without negatively affecting larval performance (1.5% w/w) was fed to bee larvae. Pollen from two transgenic plant lines expressing PSL up to 1.2% of total soluble protein and pollen from one non-transgenic line was added to the same diet and used as a pollen control. When these three pollen diets and the control diet (without added pollen) were compared, no negative effect from the pollen of the transgenic plants could be detected on larval mortality, weight, or development time. An increased weight and a reduced developmental time were recorded for larvae on all diets containing pollen when compared to the diet without pollen. PMID:18289502

  8. Continuous bioethanol production from oilseed rape straw hydrosylate using immobilised Saccharomyces cerevisiae cells.

    PubMed

    Mathew, Anil Kuruvilla; Crook, Mitch; Chaney, Keith; Humphries, Andrea Clare

    2014-02-01

    The aim of the study was to evaluate continuous bioethanol production from oilseed rape (OSR) straw hydrolysate using Saccharomyces cerevisiae cells immobilised in Lentikat® discs. The study evaluated the effect of dilution rate (0.25, 0.50, 0.75 and 1.00 h(-1)), substrate concentration (15, 22, 40 and 60 g L(-1)) and cell loading (0.03, 0.16 and 0.24 g d.c.w.mL(-1) Lentikat®) on bioethanol production. Volumetric productivity was found to increase with increasing substrate concentration from 15 g L(-1) to 60 g L(-1). A maximum volumetric productivity of 12.88 g L(-1)h(-1) was achieved at a substrate concentration of 60 g L(-1) and at a dilution rate of 0.5h(-1). An overall mass balance for bioethanol production was created to determine the energy recovery from bioethanol and concluded that a biorefinery approach might be the most appropriate option for maximising the energy recovery from OSR straw. PMID:24406845

  9. Evidence for pollinator cost and farming benefits of neonicotinoid seed coatings on oilseed rape

    PubMed Central

    Budge, G. E.; Garthwaite, D.; Crowe, A.; Boatman, N. D.; Delaplane, K. S.; Brown, M. A.; Thygesen, H. H.; Pietravalle, S.

    2015-01-01

    Chronic exposure to neonicotinoid insecticides has been linked to reduced survival of pollinating insects at both the individual and colony level, but so far only experimentally. Analyses of large-scale datasets to investigate the real-world links between the use of neonicotinoids and pollinator mortality are lacking. Moreover, the impacts of neonicotinoid seed coatings in reducing subsequent applications of foliar insecticide sprays and increasing crop yield are not known, despite the supposed benefits of this practice driving widespread use. Here, we combine large-scale pesticide usage and yield observations from oilseed rape with those detailing honey bee colony losses over an 11 year period, and reveal a correlation between honey bee colony losses and national-scale imidacloprid (a neonicotinoid) usage patterns across England and Wales. We also provide the first evidence that farmers who use neonicotinoid seed coatings reduce the number of subsequent applications of foliar insecticide sprays and may derive an economic return. Our results inform the societal discussion on the pollinator costs and farming benefits of prophylactic neonicotinoid usage on a mass flowering crop. PMID:26270806

  10. Seed Architecture Shapes Embryo Metabolism in Oilseed Rape[W][OA

    PubMed Central

    Borisjuk, Ljudmilla; Neuberger, Thomas; Schwender, Jörg; Heinzel, Nicolas; Sunderhaus, Stephanie; Fuchs, Johannes; Hay, Jordan O.; Tschiersch, Henning; Braun, Hans-Peter; Denolf, Peter; Lambert, Bart; Jakob, Peter M.; Rolletschek, Hardy

    2013-01-01

    Constrained to develop within the seed, the plant embryo must adapt its shape and size to fit the space available. Here, we demonstrate how this adjustment shapes metabolism of photosynthetic embryo. Noninvasive NMR-based imaging of the developing oilseed rape (Brassica napus) seed illustrates that, following embryo bending, gradients in lipid concentration became established. These were correlated with the local photosynthetic electron transport rate and the accumulation of storage products. Experimentally induced changes in embryo morphology and/or light supply altered these gradients and were accompanied by alterations in both proteome and metabolome. Tissue-specific metabolic models predicted that the outer cotyledon and hypocotyl/radicle generate the bulk of plastidic reductant/ATP via photosynthesis, while the inner cotyledon, being enclosed by the outer cotyledon, is forced to grow essentially heterotrophically. Under field-relevant high-light conditions, major contribution of the ribulose-1,5-bisphosphate carboxylase/oxygenase–bypass to seed storage metabolism is predicted for the outer cotyledon and the hypocotyl/radicle only. Differences between in vitro– versus in planta–grown embryos suggest that metabolic heterogeneity of embryo is not observable by in vitro approaches. We conclude that in vivo metabolic fluxes are locally regulated and connected to seed architecture, driving the embryo toward an efficient use of available light and space. PMID:23709628

  11. Barriers to gene flow from oilseed rape (Brassica napus) into populations of Sinapis arvensis.

    PubMed

    Moyes, C L; Lilley, J M; Casais, C A; Cole, S G; Haeger, P D; Dale, P J

    2002-01-01

    One concern over growing herbicide-tolerant crops is that herbicide-tolerance genes may be transferred into the weeds they are designed to control. Brassica napus (oilseed rape) has a number of wild relatives that cause weed problems and the most widespread of these is Sinapis arvensis (charlock). Sinapis arvensis seed was collected from 102 populations across the UK, within and outside B. napus-growing areas. These populations were tested for sexual compatibility with B. napus and it was found that none of them hybridized readily in the glasshouse. In contrast to previous studies, we have found that hybrids can be formed naturally with S. arvensis as the maternal parent. Six diverse B. napus cultivars (Capricorn, Drakkar, Falcon, Galaxy, Hobson and Regent) were tested for their compatibility with S. arvensis but no cultivar hybridized readily in the glasshouse. We were unable to detect gene transfer from B. napus to S. arvensis in the field, confirming the extremely low probability of hybridization predicted from the glasshouse work. PMID:11903908

  12. Expellor extracted rape and safflower oilseed meals for poultry and sheep

    SciTech Connect

    Thomas, V.M.; Katz, R.J.; Auld, D.A.; Petersen, C.F.; Sauter, E.A.

    1982-01-01

    The objective of these studies was to evaluate the feeding value of on-the-farm expellor extracted rape (RSM) and safflower (SM) oilseed meals for poultry and sheep. Rapeseed meal and SM contained 30.7 and 25.8% crude protein (CP) and 21.7 and 8.7% fat, respectively. Rapeseed meal contained a total glucosinolate concentration of 78.3 ..mu..moles/g. A 22-day feeding trial was conducted with 6-day-old chicks. Rapeseed meal and SM replaced 25 or 50% of the soybean meal (SBM) protein in isonitrogenous (23% CP), isocaloric (3250 kcal ME/kg) diets. Birds fed SBM and 25 or 50% SM consumed more (P < .01) daily feed and gained more (P < .01) per day than those fed 25 or 50% RSM. Birds fed RSM had enlarged thyroid glands in comparison to those fed SMB. Two lamb digestion trials were conducted to evaluate the effect of replacing cottonseed meal (CSM) protein with either RSM or SM on nitrogen utilization and DM digestibility. Replacing 100% of the CSM protein with RSM had no effect (P > .05) on dry matter digestibility and N utilization. Nitrogen balance studies indicate that expellor extracted SM may replace up to 75% of the CSM protein in diets for wethers. 8 tables.

  13. Evaluating the Role of Seed Treatments in Canola/Oilseed Rape Production: Integrated Pest Management, Pollinator Health, and Biodiversity.

    PubMed

    Sekulic, Gregory; Rempel, Curtis B

    2016-01-01

    The use patterns and role of insecticide seed treatments, with focus on neonicotinoid insecticides, were examined for canola/oilseed rape production in Canada and the EU. Since nearly all planted canola acres in Western Canada and, historically, a majority of planted oilseed acres in the EU, use seed treatments, it is worth examining whether broad use of insecticidal seed treatments (IST) is compatible with principles of integrated pest management (IPM). The neonicotinoid insecticide (NNI) seed treatment (NNI ST) use pattern has risen due to effective control of several early season insect pests, the most destructive being flea beetles (Phyllotreta sp.). Negative environmental impact and poor efficacy of foliar applied insecticides on flea beetles led growers to look for better alternatives. Due to their biology, predictive models have been difficult to develop for flea beetles, and, therefore, targeted application of seed treatments, as part of an IPM program, has contributed to grower profitability and overall pollinator success for canola production in Western Canada. Early evidence suggests that the recent restriction on NNI may negatively impact grower profitability and does not appear to be having positive impact on pollinator health. Further investigation on impact of NNI on individual bee vs. hive health need to be conducted. Predictive models for flea beetle emergence/feeding activity in canola/oilseed rape need to be developed, as broad acre deployment of NNI seed treatments may not be sustainable due to concerns about resistance/tolerance in flea beetles and other pest species. PMID:27527233

  14. Quantification of Plasmodiophora brassicae Using a DNA-Based Soil Test Facilitates Sustainable Oilseed Rape Production

    PubMed Central

    Wallenhammar, Ann-Charlotte; Gunnarson, Albin; Hansson, Fredrik; Jonsson, Anders

    2016-01-01

    Outbreaks of clubroot disease caused by the soil-borne obligate parasite Plasmodiophora brassicae are common in oilseed rape (OSR) in Sweden. A DNA-based soil testing service that identifies fields where P. brassicae poses a significant risk of clubroot infection is now commercially available. It was applied here in field surveys to monitor the prevalence of P. brassicae DNA in field soils intended for winter OSR production and winter OSR field experiments. In 2013 in Scania, prior to planting, P. brassicae DNA was detected in 60% of 45 fields on 10 of 18 farms. In 2014, P. brassicae DNA was detected in 44% of 59 fields in 14 of 36 farms, in the main winter OSR producing region in southern Sweden. P. brassicae was present indicative of a risk for >10% yield loss with susceptible cultivars (>1300 DNA copies g soil−1) in 47% and 44% of fields in 2013 and 2014 respectively. Furthermore, P. brassicae DNA was indicative of sites at risk of complete crop failure if susceptible cultivars were grown (>50 000 copies g−1 soil) in 14% and 8% of fields in 2013 and 2014, respectively. A survey of all fields at Lanna research station in western Sweden showed that P. brassicae was spread throughout the farm, as only three of the fields (20%) showed infection levels below the detection limit for P.brassicae DNA, while the level was >50,000 DNA copies g−1 soil in 20% of the fields. Soil-borne spread is of critical importance and soil scraped off footwear showed levels of up to 682 million spores g−1 soil. Soil testing is an important tool for determining the presence of P. brassicae and providing an indication of potential yield loss, e.g., in advisory work on planning for a sustainable OSR crop rotation. This soil test is gaining acceptance as a tool that increases the likelihood of success in precision agriculture and in applied research conducted in commercial oilseed fields and at research stations. The present application highlights the importance of prevention of

  15. Quantification of Plasmodiophora brassicae Using a DNA-Based Soil Test Facilitates Sustainable Oilseed Rape Production.

    PubMed

    Wallenhammar, Ann-Charlotte; Gunnarson, Albin; Hansson, Fredrik; Jonsson, Anders

    2016-01-01

    Outbreaks of clubroot disease caused by the soil-borne obligate parasite Plasmodiophora brassicae are common in oilseed rape (OSR) in Sweden. A DNA-based soil testing service that identifies fields where P. brassicae poses a significant risk of clubroot infection is now commercially available. It was applied here in field surveys to monitor the prevalence of P. brassicae DNA in field soils intended for winter OSR production and winter OSR field experiments. In 2013 in Scania, prior to planting, P. brassicae DNA was detected in 60% of 45 fields on 10 of 18 farms. In 2014, P. brassicae DNA was detected in 44% of 59 fields in 14 of 36 farms, in the main winter OSR producing region in southern Sweden. P. brassicae was present indicative of a risk for >10% yield loss with susceptible cultivars (>1300 DNA copies g soil(-1)) in 47% and 44% of fields in 2013 and 2014 respectively. Furthermore, P. brassicae DNA was indicative of sites at risk of complete crop failure if susceptible cultivars were grown (>50 000 copies g(-1) soil) in 14% and 8% of fields in 2013 and 2014, respectively. A survey of all fields at Lanna research station in western Sweden showed that P. brassicae was spread throughout the farm, as only three of the fields (20%) showed infection levels below the detection limit for P.brassicae DNA, while the level was >50,000 DNA copies g(-1) soil in 20% of the fields. Soil-borne spread is of critical importance and soil scraped off footwear showed levels of up to 682 million spores g(-1) soil. Soil testing is an important tool for determining the presence of P. brassicae and providing an indication of potential yield loss, e.g., in advisory work on planning for a sustainable OSR crop rotation. This soil test is gaining acceptance as a tool that increases the likelihood of success in precision agriculture and in applied research conducted in commercial oilseed fields and at research stations. The present application highlights the importance of prevention of

  16. Rapeseed species and environmental concerns related to loss of seeds of genetically modified oilseed rape in Japan.

    PubMed

    Nishizawa, Toru; Tamaoki, Masanori; Aono, Mitsuko; Kubo, Akihiro; Saji, Hikaru; Nakajima, Nobuyoshi

    2010-01-01

    Feral rapeseed in Japan consists of Brassica rapa, B. juncea and B. napus, mostly produced by escape from crops. Brassica rapa and B. juncea were introduced from abroad long ago as leaf and root vegetables and as an oil crop and breeders have developed various cultivars. Brassica napus was introduced in the late 1800s, mainly as an oil crop. Rapeseed production in Japan is low, and most demand is met by imports from Canada (94.4% of the 2009 trade volume). Recently, spontaneous B. napus, including genetically modified (GM) herbicide-resistant individuals, has been detected along Japanese roads, probably originating from seeds lost during transportation of imports. As GM oilseed production increases abroad, the probability of escape of GM oilseed rape in Japan will increase, raising environmental biosafety concerns related to the impact of feral rapeseed on heirloom brassicaceous crops. In this paper, we review the history of rapeseed introduction in Japan and future concerns. PMID:21844669

  17. Distance from forest edge affects bee pollinators in oilseed rape fields.

    PubMed

    Bailey, Samantha; Requier, Fabrice; Nusillard, Benoît; Roberts, Stuart P M; Potts, Simon G; Bouget, Christophe

    2014-02-01

    Wild pollinators have been shown to enhance the pollination of Brassica napus (oilseed rape) and thus increase its market value. Several studies have previously shown that pollination services are greater in crops adjoining forest patches or other seminatural habitats than in crops completely surrounded by other crops. In this study, we investigated the specific importance of forest edges in providing potential pollinators in B. napus fields in two areas in France. Bees were caught with yellow pan traps at increasing distances from both warm and cold forest edges into B. napus fields during the blooming period. A total of 4594 individual bees, representing six families and 83 taxa, were collected. We found that both bee abundance and taxa richness were negatively affected by the distance from forest edge. However, responses varied between bee groups and edge orientations. The ITD (Inter-Tegular distance) of the species, a good proxy for bee foraging range, seems to limit how far the bees can travel from the forest edge. We found a greater abundance of cuckoo bees (Nomada spp.) of Andrena spp. and Andrena spp. males at forest edges, which we assume indicate suitable nesting sites, or at least mating sites, for some abundant Andrena species and their parasites (Fig. 1). Synthesis and Applications. This study provides one of the first examples in temperate ecosystems of how forest edges may actually act as a reservoir of potential pollinators and directly benefit agricultural crops by providing nesting or mating sites for important early spring pollinators. Policy-makers and land managers should take forest edges into account and encourage their protection in the agricultural matrix to promote wild bees and their pollination services. PMID:24634722

  18. Distance from forest edge affects bee pollinators in oilseed rape fields

    PubMed Central

    Bailey, Samantha; Requier, Fabrice; Nusillard, Benoît; Roberts, Stuart P M; Potts, Simon G; Bouget, Christophe

    2014-01-01

    Wild pollinators have been shown to enhance the pollination of Brassica napus (oilseed rape) and thus increase its market value. Several studies have previously shown that pollination services are greater in crops adjoining forest patches or other seminatural habitats than in crops completely surrounded by other crops. In this study, we investigated the specific importance of forest edges in providing potential pollinators in B. napus fields in two areas in France. Bees were caught with yellow pan traps at increasing distances from both warm and cold forest edges into B. napus fields during the blooming period. A total of 4594 individual bees, representing six families and 83 taxa, were collected. We found that both bee abundance and taxa richness were negatively affected by the distance from forest edge. However, responses varied between bee groups and edge orientations. The ITD (Inter-Tegular distance) of the species, a good proxy for bee foraging range, seems to limit how far the bees can travel from the forest edge. We found a greater abundance of cuckoo bees (Nomada spp.) of Andrena spp. and Andrena spp. males at forest edges, which we assume indicate suitable nesting sites, or at least mating sites, for some abundant Andrena species and their parasites (Fig. 1). Synthesis and Applications. This study provides one of the first examples in temperate ecosystems of how forest edges may actually act as a reservoir of potential pollinators and directly benefit agricultural crops by providing nesting or mating sites for important early spring pollinators. Policy-makers and land managers should take forest edges into account and encourage their protection in the agricultural matrix to promote wild bees and their pollination services. PMID:24634722

  19. Consequences of gene flow between oilseed rape (Brassica napus) and its relatives.

    PubMed

    Liu, Yongbo; Wei, Wei; Ma, Keping; Li, Junsheng; Liang, Yuyong; Darmency, Henri

    2013-10-01

    Numerous studies have focused on the probability of occurrence of gene flow between transgenic crops and their wild relatives and the likelihood of transgene escape, which should be assessed before the commercial release of transgenic crops. This review paper focuses on this issue for oilseed rape, Brassica napus L., a species that produces huge numbers of pollen grains and seeds. We analyze separately the distinct steps of gene flow: (1) pollen and seeds as vectors of gene flow; (2) spontaneous hybridization; (3) hybrid behavior, fitness cost due to hybridization and mechanisms of introgression; (4) and fitness benefit due to transgenes (e.g. herbicide resistance and Bt toxin). Some physical, biological and molecular means of transgene containment are also described. Although hybrids and first generation progeny are difficult to identify in fields and non-crop habitats, the literature shows that transgenes could readily introgress into Brassica rapa, Brassica juncea and Brassica oleracea, while introgression is expected to be rare with Brassica nigra, Hirschfeldia incana and Raphanus raphanistrum. The hybrids grow well but produce less seed than their wild parent. The difference declines with increasing generations. However, there is large uncertainty about the evolution of chromosome numbers and recombination, and many parameters of life history traits of hybrids and progeny are not determined with satisfactory confidence to build generic models capable to really cover the wide diversity of situations. We show that more studies are needed to strengthen and organize biological knowledge, which is a necessary prerequisite for model simulations to assess the practical and evolutionary outputs of introgression, and to provide guidelines for gene flow management. PMID:23987810

  20. Seed Spillage from Grain Trailers on Road Verges during Oilseed Rape Harvest: An Experimental Survey

    PubMed Central

    Bailleul, Diane; Ollier, Sébastien; Huet, Sylvie; Gardarin, Antoine; Lecomte, Jane

    2012-01-01

    Context Anthropogenic vectors enhance the natural dispersal capacity of plant seeds significantly in terms of quantity and distance. Human-mediated seed dispersal (i.e. anthropochory) greatly increases the dispersal of crop species across agroecosystems. In the case of oilseed rape (OSR), spillage of seeds from grain trailers during harvest has never been quantified. Methods Our experimental approach involved establishing 85 seed trap-sites on the road verges of an agricultural area around the grain silo of Selommes (Loir-et-Cher, France). We recorded OSR spillage during harvest and applied a linear model to the data. Results The amount of seed spilled was related positively to the area of the OSR fields served by the road, whereas the amount of seed spilled decreased with other variables, such as distance from the trap-site to the verge of the road and to the nearest field. The distance to the grain silo, through local and regional effects, affected seed loss. Local effects from fields adjacent to the road resulted in a cumulative spillage on one-lane roads. On two-lane roads, spillage was nearly constant whatever the distance to the silo due to a mixture of these local effects and of grain trailers that joined the road from more distant fields. From the data, we predicted the number of seeds lost from grain trailers on one road verge in the study area. We predicted a total spillage of 2.05×106 seeds (±4.76×105) along the road length, which represented a mean of 404±94 seeds per m2. Conclusion Containment of OSR seeds will always be challenging. However, seed spillage could be reduced if grain trailers were covered and filled with less seed. Reducing distances travelled between fields and silos could also limit seed loss. PMID:22427873

  1. Root length densities of UK wheat and oilseed rape crops with implications for water capture and yield

    PubMed Central

    White, Charlotte A.; Sylvester-Bradley, Roger; Berry, Peter M.

    2015-01-01

    Root length density (RLD) was measured to 1 m depth for 17 commercial crops of winter wheat (Triticum aestivum) and 40 crops of winter oilseed rape [Brassica napus; oilseed rape (OSR)] grown in the UK between 2004 and 2013. Taking the critical RLD (cRLD) for water capture as 1cm cm–3, RLDs appeared inadequate for full water capture on average below a depth of 0.32 m for winter wheat and below 0.45 m for OSR. These depths compare unfavourably (for wheat) with average depths of ‘full capture’ of 0.86 m and 0.48 m, respectively, determined for three wheat crops and one OSR crop studied in the 1970s and 1980s, and treated as references here. A simple model of water uptake and yield indicated that these shortfalls in wheat and OSR rooting compared with the reference data might be associated with shortfalls of up to 3.5 t ha–1 and 1.2 t ha–1, respectively, in grain yields under water-limited conditions, as increasingly occur through climate change. Coupled with decreased summer rainfall, poor rooting of modern arable crops could explain much of the yield stagnation that has been observed on UK farms since the 1990s. Methods of monitoring and improving rooting under commercial conditions are reviewed and discussed. PMID:25750427

  2. Development and application of SINE multilocus and quantitative genetic markers to study oilseed rape (Brassica napus L.) crops.

    PubMed

    Allnutt, T R; Roper, K; Henry, C

    2008-01-23

    A genetic marker system based on the S1 Short Interspersed Elements (SINEs) in the important commercial crop, oilseed rape ( Brassica napus L.) has been developed. SINEs provided a successful multilocus, dominant marker system that was capable of clearly delineating winter- and spring-type crop varieties. Sixteen of 20 varieties tested showed unique profiles from the 17 polymorphic SINE markers generated. The 3' or 5' flank region of nine SINE markers were cloned, and DNA was sequenced. In addition, one putative pre-transposition SINE allele was cloned and sequenced. Two SINE flanking sequences were used to design real-time PCR assays. These quantitative SINE assays were applied to study the genetic structure of eight fields of oilseed rape crops. Studied fields were more genetically diverse than expected for the chosen loci (mean H T = 0.23). The spatial distribution of SINE marker frequencies was highly structured in some fields, suggesting locations of volunteer impurities within the crop. In one case, the assay identified a mislabeling of the crop variety. SINE markers were a useful tool for crop genetics, phylogenetics, variety identification, and purity analysis. The use and further application of quantitative, real-time PCR markers are discussed. PMID:18092752

  3. Root length densities of UK wheat and oilseed rape crops with implications for water capture and yield.

    PubMed

    White, Charlotte A; Sylvester-Bradley, Roger; Berry, Peter M

    2015-04-01

    Root length density (RLD) was measured to 1 m depth for 17 commercial crops of winter wheat (Triticum aestivum) and 40 crops of winter oilseed rape [Brassica napus; oilseed rape (OSR)] grown in the UK between 2004 and 2013. Taking the critical RLD (cRLD) for water capture as 1cm cm(-3), RLDs appeared inadequate for full water capture on average below a depth of 0.32 m for winter wheat and below 0.45 m for OSR. These depths compare unfavourably (for wheat) with average depths of 'full capture' of 0.86 m and 0.48 m, respectively, determined for three wheat crops and one OSR crop studied in the 1970s and 1980s, and treated as references here. A simple model of water uptake and yield indicated that these shortfalls in wheat and OSR rooting compared with the reference data might be associated with shortfalls of up to 3.5 t ha(-1) and 1.2 t ha(-1), respectively, in grain yields under water-limited conditions, as increasingly occur through climate change. Coupled with decreased summer rainfall, poor rooting of modern arable crops could explain much of the yield stagnation that has been observed on UK farms since the 1990s. Methods of monitoring and improving rooting under commercial conditions are reviewed and discussed. PMID:25750427

  4. Reprogramming the Phenylpropanoid Metabolism in Seeds of Oilseed Rape by Suppressing the Orthologs of REDUCED EPIDERMAL FLUORESCENCE11[W

    PubMed Central

    Mittasch, Juliane; Böttcher, Christoph; Frolov, Andrej; Strack, Dieter; Milkowski, Carsten

    2013-01-01

    As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene REDUCED EPIDERMAL FLUORESCENCE1 (REF1) encoding a coniferaldehyde/sinapaldehyde dehydrogenase. The enzyme is involved in the formation of ferulate and sinapate from the corresponding aldehydes, thereby linking lignin and hydroxycinnamate biosynthesis as a potential branch-point enzyme. We used RNA interference to silence REF1 genes in seeds of oilseed rape. Nontargeted metabolite profiling showed that BnREF1-suppressing seeds produced a novel chemotype characterized by reduced levels of sinapate esters, the appearance of conjugated monolignols, dilignols, and trilignols, altered accumulation patterns of kaempferol glycosides, and changes in minor conjugates of caffeate, ferulate, and 5-hydroxyferulate. BnREF1 suppression affected the level of minor sinapate conjugates more severely than that of the major component sinapine. Mapping of the changed metabolites onto the phenylpropanoid metabolic network revealed partial redirection of metabolic sequences as a major impact of BnREF1 suppression. PMID:23424250

  5. Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China.

    PubMed

    Xu, D; Pan, Y; Zhang, H; Li, X; Dai, Y; Cao, S; Gao, Z

    2015-01-01

    Stem rot caused by Sclerotinia sclerotiorum is a devastating disease of oilseed rape (Brassica napus) in Anhui Province of China. The fungicide carbendazim (methyl benzimidazole-2-yl carbamate; MBC) has been used to control this fungal disease since the 1980s. In the present study, 74 isolates of S. sclerotiorum from 13 regions of Anhui were collected, and the sensitivities of these isolates to MBC were examined to monitor fungicide resistance. We found that 22 of the 74 isolates showed resistance to MBC, indicating that S. sclerotiorum has developed resistance in parts of Anhui Province. PCR assays and DNA sequence analysis showed that isolates with high MBC resistance had a point mutation at position 198 in the β-tubulin gene that caused a glutamic acid-to-alanine change in the protein. The β-tubulin gene in low-resistance isolates did not have the mutation. These results indicate that the mutation in β-tubulin gene may be associated with high MBC resistance in S. sclerotiorum. The present study also found no correlation between MBC resistance and pathogenicity of S. sclerotiorum isolates, suggesting that the pathogenicity of S. sclerotiorum isolates on oilseed rape did not vary with MBC resistance status. PMID:26681009

  6. Characterization of senescence-associated protease activities involved in the efficient protein remobilization during leaf senescence of winter oilseed rape.

    PubMed

    Poret, Marine; Chandrasekar, Balakumaran; van der Hoorn, Renier A L; Avice, Jean-Christophe

    2016-05-01

    Oilseed rape (Brassica napus L.) is a crop plant characterized by a poor nitrogen (N) use efficiency that is mainly due to low N remobilization efficiency during the sequential leaf senescence of the vegetative stage. As a high leaf N remobilization efficiency was strongly linked to a high remobilization of proteins during leaf senescence of rapeseed, our objective was to identify senescence-associated protease activities implicated in the protein degradation. To reach this goal, leaf senescence processes and protease activities were investigated in a mature leaf becoming senescent in plants subjected to ample or low nitrate supply. The characterization of protease activities was performed by using in vitro analysis of RuBisCO degradation with or without inhibitors of specific protease classes followed by a protease activity profiling using activity-dependent probes. As expected, the mature leaf became senescent regardless of the nitrate treatment, and nitrate limitation enhanced the senescence processes associated with an enhanced degradation of soluble proteins. The characterization of protease activities revealed that: (i) aspartic proteases and the proteasome were active during senescence regardless of nitrate supply, and (ii) the activities of serine proteases and particularly cysteine proteases (Papain-like Cys proteases and vacuolar processing enzymes) increased when protein remobilization associated with senescence was accelerated by nitrate limitation. Short statement: Serine and particularly cysteine proteases (both PLCPs and VPEs) seem to play a crucial role in the efficient protein remobilization when leaf senescence of oilseed rape was accelerated by nitrate limitation. PMID:26993244

  7. The use of Pseudomonas fluorescens P13 to control sclerotinia stem rot (Sclerotinia sclerotiorum) of oilseed rape.

    PubMed

    Li, Hui; Li, Huaibo; Bai, Yan; Wang, Jing; Nie, Ming; Li, Bo; Xiao, Ming

    2011-12-01

    Sclerotinia stem rot (SSR) caused by the fungus Sclerotinia sclerotiorum has been an increasing threat to oilseed rape (Brassica napus L.) cultivation. Efficient and environment-friendly treatments are much needed. Here we focus on microbial control. The Pseudomonas fluorescens P13 that was isolated from oilseed rape cultivation soil, proved to be a useful biocontrol strain for application. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that it was P. fluorescens P13 and that it had a broad antagonistic spectrum, significantly lessening the mycelial growth of S. sclerotiorum by 84.4% and suppressing sclerotial formation by 95-100%. Scanning electron microscopy studies attested that P13 deformed S. sclerotiorum mycelia when they were cultured together. P13 did not produce chitinase but did produce hydrogen cyanide (HCN) which was likely one of the antagonistic mechanisms. The density of P13 remained at a high level (≥10(6) CFU/ml) during 5 weeks in the rhizosphere soil and roots. P13 reduced SSR severity at least by 59% in field studies and also promoted seedling growth (p<0.05) at the seedling stage. From these data, our work provided evidence that P13 could be a good alternative biological resource for biocontrol of S. sclerotiorum. PMID:22203550

  8. Xylem transport and gene expression play decisive roles in cadmium accumulation in shoots of two oilseed rape cultivars (Brassica napus).

    PubMed

    Wu, Zhichao; Zhao, Xiaohu; Sun, Xuecheng; Tan, Qiling; Tang, Yafang; Nie, Zhaojun; Hu, Chengxiao

    2015-01-01

    Cadmium (Cd) is a toxic metal which harms human health through food chains. The mechanisms underlying Cd accumulation in oilseed rape are still poorly understood. Here, we investigated the physiological and genetic processes involved in Cd uptake and transport of two oilseed rape cultivars (Brassica napus). L351 accumulates more Cd in shoots but less in roots than L338. A scanning ion-selective electrode technique (SIET) and uptake kinetics of Cd showed that roots were not responsible for the different Cd accumulation in shoots since L351 showed a lower Cd uptake ability. However, concentration-dependent and time-dependent dynamics of Cd transport by xylem showed L351 exhibited a superordinate capacity of Cd translocation to shoots. Additionally, the Cd concentrations of shoots and xylem sap showed a great correlation in both cultivars. Furthermore, gene expression levels related to Cd uptake by roots (IRT1) and Cd transport by xylem (HMA2 and HMA4) were consistent with the tendencies of Cd absorption and transport at the physiological level respectively. In other words, L351 had stronger gene expression for Cd transport but lower for Cd uptake. Overall, results revealed that the process of Cd translocation to shoots is a determinative factor for Cd accumulation in shoots, both at physiological and genetic levels. PMID:25460764

  9. Draft Genome Sequence of the Beneficial Rhizobacterium Pseudomonas fluorescens DSM 8569, a Natural Isolate of Oilseed Rape (Brassica napus)

    PubMed Central

    Nesemann, Kai; Braus-Stromeyer, Susanna A.; Thuermer, Andrea; Daniel, Rolf

    2015-01-01

    Pseudomonas fluorescens DSM 8569 represents a natural isolate of the rhizosphere of oilseed rape (Brassica napus) in Germany and possesses antagonistic potential toward the fungal pathogen Verticillium. We report here the draft genome sequence of strain DSM 8569, which comprises 5,914 protein-coding sequences. PMID:25814596

  10. Assays of the production of harmful substances by genetically modified oilseed rape (Brassica napus L.) plants in accordance with regulations for evaluating the impact on biodiversity in Japan.

    PubMed

    Asanuma, Yoko; Jinkawa, Tomoe; Tanaka, Hidenori; Gondo, Takahiro; Zaita, Norihiro; Akashi, Ryo

    2011-02-01

    Environmental risk assessment of transgenic crops is implemented under the Cartagena Protocol domestic law in accordance with guidelines for implementing the assessment established by the Ministry of Agriculture, Forestry and Fisheries (MAFF) and the Ministry of Environment (MOE) in Japan. Environmental risk assessments of transgenic crops are implemented based on the concept of 'substantial equivalence' to conventional crops. A unique requirement in Japan to monitor the production of harmful substances, or allelochemicals, is unparalleled in other countries. The potential for allelochemicals to be secreted from the roots of transgenic crops to affect other plants or soil microflora or for substances in the plant body to affect other plants after dying out must be evaluated. We evaluated the allelopathic potential of seven transgenic oilseed rape (Brassica napus L.) lines that express glufosinate tolerance in terms of substantial equivalence to conventional oilseed rape lines, and established evaluation methods. Our results indicate no potential production of allelochemicals for any of the seven transgenic oilseed rape lines compared with conventional oilseed rape lines. PMID:20607604

  11. Formulations of the endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiorum on oilseed rape and improve plant vigor in field trials conducted at separate locations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sclerotinia sclerotiorum causes serious yield losses in crops in The People’s Republic of China. Two formulations of oilseed rape seed containing the endophytic bacterium Bacillus subtilis Tu-100 were evaluated for suppression of this pathogen in field trials conducted at two independent locations....

  12. Ecological Variation in Response to Mass-Flowering Oilseed Rape and Surrounding Landscape Composition by Members of a Cryptic Bumblebee Complex

    PubMed Central

    Stanley, Dara A.; Knight, Mairi E.; Stout, Jane C.

    2013-01-01

    The Bombus sensu stricto species complex is a widespread group of cryptic bumblebee species which are important pollinators of many crops and wild plants. These cryptic species have, until now, largely been grouped together in ecological studies, and so little is known about their individual colony densities, foraging ranges or habitat requirements, which can be influenced by land use at a landscape scale. We used mass-flowering oilseed rape fields as locations to sample bees of this complex, as well as the second most common visitor to oilseed rape B. lapidarius, and molecular RFLP methods to distinguish between the cryptic species. We then used microsatellite genotyping to identify sisters and estimate colony densities, and related both proportions of cryptic species and their colony densities to the composition of the landscape surrounding the fields. We found B. lucorum was the most common member of the complex present in oilseed rape followed by B. terrestris. B. cryptarum was also present in all but one site, with higher proportions found in the east of the study area. High numbers of bumblebee colonies were estimated to be using oilseed rape fields as a forage resource, with B. terrestris colony numbers higher than previous estimates from non-mass-flowering fields. We also found that the cryptic species responded differently to surrounding landscape composition: both relative proportions of B. cryptarum in samples and colony densities of B. lucorum were negatively associated with the amount of arable land in the landscape, while proportions and colony densities of other species did not respond to landscape variables at the scale measured. This suggests that the cryptic species have different ecological requirements (which may be scale-dependent) and that oilseed rape can be an important forage resource for many colonies of bumblebees. Given this, we recommend sustainable management of this crop to benefit bumblebees. PMID:23840338

  13. Application of Visible and Near-Infrared Hyperspectral Imaging to Determine Soluble Protein Content in Oilseed Rape Leaves

    PubMed Central

    Zhang, Chu; Liu, Fei; Kong, Wenwen; He, Yong

    2015-01-01

    Visible and near-infrared hyperspectral imaging covering spectral range of 380–1030 nm as a rapid and non-destructive method was applied to estimate the soluble protein content of oilseed rape leaves. Average spectrum (500–900 nm) of the region of interest (ROI) of each sample was extracted, and four samples out of 128 samples were defined as outliers by Monte Carlo-partial least squares (MCPLS). Partial least squares (PLS) model using full spectra obtained dependable performance with the correlation coefficient (rp) of 0.9441, root mean square error of prediction (RMSEP) of 0.1658 mg/g and residual prediction deviation (RPD) of 2.98. The weighted regression coefficient (Bw), successive projections algorithm (SPA) and genetic algorithm-partial least squares (GAPLS) selected 18, 15, and 16 sensitive wavelengths, respectively. SPA-PLS model obtained the best performance with rp of 0.9554, RMSEP of 0.1538 mg/g and RPD of 3.25. Distribution of protein content within the rape leaves were visualized and mapped on the basis of the SPA-PLS model. The overall results indicated that hyperspectral imaging could be used to determine and visualize the soluble protein content of rape leaves. PMID:26184198

  14. Impact of oilseed rape expressing the insecticidal serine protease inhibitor, mustard trypsin inhibitor-2 on the beneficial predator Pterostichus madidus.

    PubMed

    Ferry, N; Jouanin, L; Ceci, L R; Mulligan, E A; Emami, K; Gatehouse, J A; Gatehouse, A M R

    2005-01-01

    Abstract Insect-resistant transgenic plants have been suggested to have deleterious effects on beneficial predators feeding on crop pests, through transmission of the transgene product by the pest to the predator. To test this hypothesis, effects of oilseed rape expressing the serine protease inhibitor, mustard trypsin inhibitor -2 (MTI-2), on the predatory ground beetle Pterostichus madidus were investigated, using diamondback moth, Plutella xylostella as the intermediary pest species. As expected, oilseed rape expressing MTI-2 had a deleterious effect on the development and survival of the pest. However, incomplete pest mortality resulted in survivors being available to predators at the next trophic level, and inhibition studies confirmed the presence of biologically active transgene product in pest larvae. Characterization of proteolytic digestive enzymes of P. madidus demonstrated that adults utilize serine proteases with trypsin-like and chymotrypsin-like specificities; the former activity was completely inhibited by MTI-2 in vitro. When P. madidus consumed prey reared on MTI-2 expressing plants over the reproductive period in their life cycle, no significant effects upon survival were observed as a result of exposure to the inhibitor. However, there was a short-term significant inhibition of weight gain in female beetles fed unlimited prey containing MTI-2, with a concomitant reduction of prey consumption. Biochemical analyses showed that the inhibitory effects of MTI-2 delivered via prey on gut proteolysis in the carabid decreased with time of exposure, possibly resulting from up-regulation of inhibitor-insensitive proteases. Of ecological significance, consumption of MTI-2 dosed prey had no detrimental effects on reproductive fitness of adult P. madidus. PMID:15643975

  15. Impact of oilseed rape expressing the insecticidal cysteine protease inhibitor oryzacystatin on the beneficial predator Harmonia axyridis (multicoloured Asian ladybeetle).

    PubMed

    Ferry, N; Raemaekers, R J M; Majerus, M E N; Jouanin, L; Port, G; Gatehouse, J A; Gatehouse, A M R

    2003-02-01

    Insect-resistant transgenic plants have been suggested to have deleterious effects on beneficial predators through transmission of the transgene product by the pest to the predator. To test this hypothesis, effects of oilseed rape expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1) on the predatory ladybird Harmonia axyridis were investigated using diamondback moth Plutella xylostella as the pest species. As expected, oilseed rape expressing OC-1 had no effects on either development or survival of the pest, which utilizes serine digestive proteases. Immunoassays confirmed accumulation of the transgene product in pest larval tissues at levels of up to 3 ng per gut. Characterization of proteolytic digestive enzymes of H. axyridis demonstrated that larvae and adults utilize cysteine and aspartic proteases; the former activity was completely inhibited by oryzacystatin in vitro. However, when H. axyridis larvae consumed prey reared on OC-1 expressing plants over their entire life cycle, no significant effects upon survival or overall development were observed. The inhibitor initially stimulated development, with a shortening of the developmental period of the second instar by 27% (P < 0.0001) accompanied by a 36% increase in weight of second instar larvae (P = 0.007). OC-1 had no detrimental effects on reproductive fitness of adult H. axyridis. Interestingly there was a significant increase in consumption of OC-1 dosed prey. The results show that prey reared on transgenic plants expressing a protein which inhibited ladybird digestive enzymes in vitro had no effects in vivo; the ladybird was able to up-regulate digestive proteases in response to the inhibitor. PMID:12535099

  16. Complex Formation of Myrosinase Isoenzymes in Oilseed Rape Seeds Are Dependent on the Presence of Myrosinase-Binding Proteins1

    PubMed Central

    Eriksson, Susanna; Andréasson, Erik; Ekbom, Barbara; Granér, Georg; Pontoppidan, Bo; Taipalensuu, Jan; Zhang, Jiaming; Rask, Lars; Meijer, Johan

    2002-01-01

    The enzyme myrosinase (EC 3.2.3.1) degrades the secondary compounds glucosinolates upon wounding and serves as a defense to generalist pests in Capparales. Certain myrosinases are present in complexes together with other proteins such as myrosinase-binding proteins (MBP) in extracts of oilseed rape (Brassica napus) seeds. Immunhistochemical analysis of wild-type seeds showed that MBPs were present in most cells but not in the myrosin cells, indicating that the complex formation observed in extracts is initiated upon tissue disruption. To study the role of MBP in complex formation and defense, oilseed rape antisense plants lacking the seed MBPs were produced. Western blotting and immunohistochemical staining confirmed depletion of MBP in the transgenic seeds. The exclusive expression of myrosinase in idioblasts (myrosin cells) of the seed was not affected by the down-regulation of MBP. Using size-exclusion chromatography, we have shown that myrosinases with subunit molecular masses of 62 to 70 kD were present as free dimers from the antisense seed extract, whereas in the wild type, they formed complexes. In accordance with this, MBPs are necessary for myrosinase complex formation of the 62- to 70-kD myrosinases. The product formed from sinalbin hydrolysis by myrosinase was the same whether MBP was present or not. The performance of a common beetle generalist (Tenebrio molitor) fed with seeds, herbivory by flea beetles (Phyllotreta undulata) on cotyledons, or growth rate of the Brassica fungal pathogens Alternaria brassicae or Lepthosphaeria maculans in the presence of seed extracts were not affected by the down-regulation of MBP, leaving the physiological function of this protein family open. PMID:12177471

  17. Complex formation of myrosinase isoenzymes in oilseed rape seeds are dependent on the presence of myrosinase-binding proteins.

    PubMed

    Eriksson, Susanna; Andréasson, Erik; Ekbom, Barbara; Granér, Georg; Pontoppidan, Bo; Taipalensuu, Jan; Zhang, Jiaming; Rask, Lars; Meijer, Johan

    2002-08-01

    The enzyme myrosinase (EC 3.2.3.1) degrades the secondary compounds glucosinolates upon wounding and serves as a defense to generalist pests in Capparales. Certain myrosinases are present in complexes together with other proteins such as myrosinase-binding proteins (MBP) in extracts of oilseed rape (Brassica napus) seeds. Immunhistochemical analysis of wild-type seeds showed that MBPs were present in most cells but not in the myrosin cells, indicating that the complex formation observed in extracts is initiated upon tissue disruption. To study the role of MBP in complex formation and defense, oilseed rape antisense plants lacking the seed MBPs were produced. Western blotting and immunohistochemical staining confirmed depletion of MBP in the transgenic seeds. The exclusive expression of myrosinase in idioblasts (myrosin cells) of the seed was not affected by the down-regulation of MBP. Using size-exclusion chromatography, we have shown that myrosinases with subunit molecular masses of 62 to 70 kD were present as free dimers from the antisense seed extract, whereas in the wild type, they formed complexes. In accordance with this, MBPs are necessary for myrosinase complex formation of the 62- to 70-kD myrosinases. The product formed from sinalbin hydrolysis by myrosinase was the same whether MBP was present or not. The performance of a common beetle generalist (Tenebrio molitor) fed with seeds, herbivory by flea beetles (Phyllotreta undulata) on cotyledons, or growth rate of the Brassica fungal pathogens Alternaria brassicae or Lepthosphaeria maculans in the presence of seed extracts were not affected by the down-regulation of MBP, leaving the physiological function of this protein family open. PMID:12177471

  18. N-(2-bromophenyl)-2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamine, a new selective postemergent herbicide for weed control in winter oilseed rape.

    PubMed

    Wu, Jun; Cheng, Jie; Lu, Long

    2006-08-01

    N-(2-Bromophenyl)-2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamine is a highly active herbicide, which belongs to a novel class of chemistry. The compound is de novo synthesized in good yield, and the structure is confirmed by (1)H NMR, IR, MS, microanalysis, and X-ray. Its herbicidal activity is assessed under greenhouse conditions. It is effective against many grass weed species, as well as broadleaf weeds, under greenhouse conditions. Field trials indicate that it controls major weeds with a good tolerance on oilseed rape by postemergence application at rates of 15-90 g of active ingredient/ha. This compound possesses low mammalian toxicity and favorable environmental profile. These results suggest that the compound has potential as a new selective postemergent herbicide in winter oilseed rape. PMID:16881700

  19. Functional characterization of NAC55 transcription factor from oilseed rape (Brassica napus L.) as a novel transcriptional activator modulating reactive oxygen species accumulation and cell death.

    PubMed

    Niu, Fangfang; Wang, Chen; Yan, Jingli; Guo, Xiaohua; Wu, Feifei; Yang, Bo; Deyholos, Michael K; Jiang, Yuan-Qing

    2016-09-01

    NAC transcription factors (TFs) are plant-specific and play important roles in development, responses to biotic and abiotic cues and hormone signaling. So far, only a few NAC genes have been reported to regulate cell death. In this study, we identified and characterized a NAC55 gene isolated from oilseed rape (Brassica napus L.). BnaNAC55 responds to multiple stresses, including cold, heat, abscisic acid (ABA), jasmonic acid (JA) and a necrotrophic fungal pathogen Sclerotinia sclerotiorum. BnaNAC55 has transactivation activity and is located in the nucleus. BnaNAC55 is able to form homodimers in planta. Unlike ANAC055, full-length BnaNAC55, but not either the N-terminal NAC domain or C-terminal regulatory domain, induces ROS accumulation and hypersensitive response (HR)-like cell death when expressed both in oilseed rape protoplasts and Nicotiana benthamiana. Furthermore, BnaNAC55 expression causes obvious nuclear DNA fragmentation. Moreover, quantitative reverse transcription PCR (qRT-PCR) analysis identified that the expression levels of multiple genes regulating ROS production and scavenging, defense response as well as senescence are significantly induced. Using a dual luciferase reporter assay, we further confirm that BnaNAC55 could activate the expression of a few ROS and defense-related gene expression. Taken together, our work has identified a novel NAC TF from oilseed rape that modulates ROS accumulation and cell death. PMID:27312204

  20. Rape

    MedlinePlus

    ... How Can I Help a Friend Who Cuts? Rape KidsHealth > For Teens > Rape Print A A A Text Size What's in ... Happens During the Medical Exam? Dealing With Feelings Rape, sometimes also called sexual assault, can happen to ...

  1. Hyperspectral and Thermal Imaging of Oilseed Rape (Brassica napus) Response to Fungal Species of the Genus Alternaria

    PubMed Central

    Baranowski, Piotr; Jedryczka, Malgorzata; Mazurek, Wojciech; Babula-Skowronska, Danuta; Siedliska, Anna; Kaczmarek, Joanna

    2015-01-01

    In this paper, thermal (8-13 µm) and hyperspectral imaging in visible and near infrared (VNIR) and short wavelength infrared (SWIR) ranges were used to elaborate a method of early detection of biotic stresses caused by fungal species belonging to the genus Alternaria that were host (Alternaria alternata, Alternaria brassicae, and Alternaria brassicicola) and non-host (Alternaria dauci) pathogens to oilseed rape (Brassica napus L.). The measurements of disease severity for chosen dates after inoculation were compared to temperature distributions on infected leaves and to averaged reflectance characteristics. Statistical analysis revealed that leaf temperature distributions on particular days after inoculation and respective spectral characteristics, especially in the SWIR range (1000-2500 nm), significantly differed for the leaves inoculated with A. dauci from the other species of Alternaria as well as from leaves of non-treated plants. The significant differences in leaf temperature of the studied Alternaria species were observed in various stages of infection development. The classification experiments were performed on the hyperspectral data of the leaf surfaces to distinguish days after inoculation and Alternaria species. The second-derivative transformation of the spectral data together with back-propagation neural networks (BNNs) appeared to be the best combination for classification of days after inoculation (prediction accuracy 90.5%) and Alternaria species (prediction accuracy 80.5%). PMID:25826369

  2. The Effects of Seed Size on Hybrids Formed between Oilseed Rape (Brassica napus) and Wild Brown Mustard (B. juncea)

    PubMed Central

    Liu, Yong-bo; Tang, Zhi-xi; Darmency, Henri; Stewart, C. Neal; Di, Kun; Wei, Wei; Ma, Ke-ping

    2012-01-01

    Background Seed size has significant implications in ecology, because of its effects on plant fitness. The hybrid seeds that result from crosses between crops and their wild relatives are often small, and the consequences of this have been poorly investigated. Here we report on plant performance of hybrid and its parental transgenic oilseed rape (Brassica napus) and wild B. juncea, all grown from seeds sorted into three seed-size categories. Methodology/Principal Findings Three seed-size categories were sorted by seed diameter for transgenic B. napus, wild B. juncea and their transgenic and non-transgenic hybrids. The seeds were sown in a field at various plant densities. Globally, small-seeded plants had delayed flowering, lower biomass, fewer flowers and seeds, and a lower thousand-seed weight. The seed-size effect varied among plant types but was not affected by plant density. There was no negative effect of seed size in hybrids, but it was correlated with reduced growth for both parents. Conclusions Our results imply that the risk of further gene flow would probably not be mitigated by the small size of transgenic hybrid seeds. No fitness cost was detected to be associated with the Bt-transgene in this study. PMID:22745814

  3. Mature green waste compost enhances growth and nitrogen uptake in wheat (Triticum aestivum L.) and oilseed rape (Brassica napus L.) through the action of water-extractable factors.

    PubMed

    Keeling, A A; McCallum, K R; Beckwith, C P

    2003-11-01

    A series of field and pot trials were carried out to determine the effects of growing wheat and oilseed rape in soils supplemented with green waste composts and provided with additional fertilisers. It was shown consistently that the response of wheat and rape to compost and fertiliser applied together was greater than the responses to the individual additives, but only when very stable compost was used (>10 months processing). Experiments with 15N-labelled fertiliser showed that wheat was able to utilise the applied N more efficiently when cultivated in the stable compost. The enhanced growth was also demonstrated in hydroponic culture of oilseed rape with water extracts of green waste compost in the presence of compound fertiliser. However the effect was rapidly lost at higher dilutions of compost extract (>3). It was concluded that water-extractable growth promoters are present in stable green waste compost, but these only have measurable activity at high concentrations. The identity of the growth promoting factors remains to be found, but the literature suggests that water-extractable humic substances or cytokinins may be involved. PMID:12895554

  4. Long-Term Monitoring of Field Trial Sites with Genetically Modified Oilseed Rape (Brassica napus L.) in Saxony-Anhalt, Germany. Fifteen Years Persistence to Date but No Spatial Dispersion.

    PubMed

    Belter, Anke

    2016-01-01

    Oilseed rape is known to persist in arable fields because of its ability to develop secondary seed dormancy in certain agronomic and environmental conditions. If conditions change, rapeseeds are able to germinate up to 10 years later to build volunteers in ensuing crops. Extrapolations of experimental data acted on the assumption of persistence periods for more than 20 years after last harvest of rapeseed. Genetically-modified oilseed rape-cultivated widely in Northern America since 1996-is assumed not to differ from its conventional form in this property. Here, experimental data are reported from official monitoring activities that verify these assumptions. At two former field trial sites in Saxony-Anhalt genetically-modified herbicide-resistant oilseed rape volunteers are found up to fifteen years after harvest. Nevertheless, spatial dispersion or establishment of GM plants outside of the field sites was not observed within this period. PMID:26784233

  5. The impact of sulfate restriction on seed yield and quality of winter oilseed rape depends on the ability to remobilize sulfate from vegetative tissues to reproductive organs

    PubMed Central

    Girondé, Alexandra; Dubousset, Lucie; Trouverie, Jacques; Etienne, Philippe; Avice, Jean-Christophe

    2014-01-01

    Our current knowledge about sulfur (S) management by winter oilseed rape to satisfy the S demand of developing seeds is still scarce, particularly in relation to S restriction. Our goals were to determine the physiological processes related to S use efficiency that led to maintain the seed yield and quality when S limitation occurred at the bolting or early flowering stages. To address these questions, a pulse-chase 34SO2−4 labeling method was carried out in order to study the S fluxes from uptake and remobilization at the whole plant level. In response of S limitation at the bolting or early flowering stages, the leaves are the most important source organ for S remobilization during reproductive stages. By combining 34S-tracer with biochemical fractionation in order to separate sulfate from other S-compounds, it appeared that sulfate was the main form of S remobilized in leaves at reproductive stages and that tonoplastic SULTR4-type transporters were specifically involved in the sulfate remobilisation in case of low S availability. In response to S limitation at the bolting stage, the seed yield and quality were dramatically reduced compared to control plants. These data suggest that the increase of both S remobilization from source leaves and the root proliferation in order to maximize sulfate uptake capacities, were not sufficient to maintain the seed yield and quality. When S limitation occurred at the early flowering stage, oilseed rape can optimize the mobilization of sulfate reserves from vegetative organs (leaves and stem) to satisfy the demand of seeds and maintain the seed yield and quality. Our study also revealed that the stem may act as a transient storage organ for remobilized S coming from source leaves before its utilization by seeds. The physiological traits (S remobilization, root proliferation, transient S storage in stem) observed under S limitation could be used in breeding programs to select oilseed rape genotypes with high S use efficiency

  6. Sulphur limitation provokes physiological and leaf proteome changes in oilseed rape that lead to perturbation of sulphur, carbon and oxidative metabolisms

    PubMed Central

    2013-01-01

    Background The decline in industrial emissions of sulphur (S) has led to a sulphate depletion in soil resulting in an alteration of crop performance. In oilseed rape, an S deficiency dramatically reduced the seed yield and/or quality. Paradoxically, little is known about the impact of sulphate limitation on oilseed rape leaf metabolism, despite it being a key determinant of growth. In order to identify the metabolic processes involved in the oilseed rape response to S restriction, an analysis of the young leaf proteome combined with a physiological study was carried out at the vegetative stage. Results S limitation does not significantly reduce the total shoot biomass but inhibits growth and photosynthesis of young leaves. This photosynthesis decline is not due to a decrease in chlorophyll content, which remains similar to Control. The increase in anthocyanins and H2O2 content in young leaves of S-limited plants suggests that S restriction leads to an oxidative stress. Proteomic analysis at 35 d of S limitation also revealed the induction of 12-oxophitodienoate reductase and ACC synthase, respectively involved in jasmonate and ethylene biosynthesis, two phytohormones that could be implicated in oxidative stress. Proteins involved in photosynthesis and carbon metabolism were also modulated by S restriction. In particular, the decrease in plastocyanin and ferredoxin–NADP reductase suggests that H2O2 accumulation is associated with perturbation of the photosynthetic electron transport chain. The accumulation of chloroplastic Cu-Zn SOD reinforces the idea that an oxidative stress probably occurs in the chloroplast. Proteomic results suggest that the maintenance of chlorophyll in S-limited conditions is related to an accumulation of Water Soluble Chlorophyll binding Proteins, involved in the protection of chlorophyll against ROS. The accumulation of the catalytic α–subunit of chloroplastic ATP synthase suggests that energy production is maintained. Conclusion S

  7. Impact of Shortened Crop Rotation of Oilseed Rape on Soil and Rhizosphere Microbial Diversity in Relation to Yield Decline

    PubMed Central

    Hilton, Sally; Bennett, Amanda J.; Keane, Gary; Bending, Gary D.; Chandler, David; Stobart, Ron; Mills, Peter

    2013-01-01

    Oilseed rape (OSR) grown in monoculture shows a decline in yield relative to virgin OSR of up to 25%, but the mechanisms responsible are unknown. A long term field experiment of OSR grown in a range of rotations with wheat was used to determine whether shifts in fungal and bacterial populations of the rhizosphere and bulk soil were associated with the development of OSR yield decline. The communities of fungi and bacteria in the rhizosphere and bulk soil from the field experiment were profiled using terminal restriction fragment length polymorphism (TRFLP) and sequencing of cloned internal transcribed spacer regions and 16S rRNA genes, respectively. OSR cropping frequency had no effect on rhizosphere bacterial communities. However, the rhizosphere fungal communities from continuously grown OSR were significantly different to those from other rotations. This was due primarily to an increase in abundance of two fungi which showed 100% and 95% DNA identity to the plant pathogens Olpidium brassicae and Pyrenochaeta lycopersici, respectively. Real-time PCR confirmed that there was significantly more of these fungi in the continuously grown OSR than the other rotations. These two fungi were isolated from the field and used to inoculate OSR and Brassica oleracea grown under controlled conditions in a glasshouse to determine their effect on yield. At high doses, Olpidium brassicae reduced top growth and root biomass in seedlings and reduced branching and subsequent pod and seed production. Pyrenochaeta sp. formed lesions on the roots of seedlings, and at high doses delayed flowering and had a negative impact on seed quantity and quality. PMID:23573215

  8. A High-Density Genetic Map Identifies a Novel Major QTL for Boron Efficiency in Oilseed Rape (Brassica napus L.)

    PubMed Central

    Wang, Xiaohua; Zhao, Hua; Shi, Lei; Xu, Fangsen

    2014-01-01

    Low boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs) for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC) were analyzed using a doubled haploid (DH) population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs) assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs) and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14–46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus. PMID:25375356

  9. Impact of shortened crop rotation of oilseed rape on soil and rhizosphere microbial diversity in relation to yield decline.

    PubMed

    Hilton, Sally; Bennett, Amanda J; Keane, Gary; Bending, Gary D; Chandler, David; Stobart, Ron; Mills, Peter

    2013-01-01

    Oilseed rape (OSR) grown in monoculture shows a decline in yield relative to virgin OSR of up to 25%, but the mechanisms responsible are unknown. A long term field experiment of OSR grown in a range of rotations with wheat was used to determine whether shifts in fungal and bacterial populations of the rhizosphere and bulk soil were associated with the development of OSR yield decline. The communities of fungi and bacteria in the rhizosphere and bulk soil from the field experiment were profiled using terminal restriction fragment length polymorphism (TRFLP) and sequencing of cloned internal transcribed spacer regions and 16S rRNA genes, respectively. OSR cropping frequency had no effect on rhizosphere bacterial communities. However, the rhizosphere fungal communities from continuously grown OSR were significantly different to those from other rotations. This was due primarily to an increase in abundance of two fungi which showed 100% and 95% DNA identity to the plant pathogens Olpidium brassicae and Pyrenochaeta lycopersici, respectively. Real-time PCR confirmed that there was significantly more of these fungi in the continuously grown OSR than the other rotations. These two fungi were isolated from the field and used to inoculate OSR and Brassica oleracea grown under controlled conditions in a glasshouse to determine their effect on yield. At high doses, Olpidium brassicae reduced top growth and root biomass in seedlings and reduced branching and subsequent pod and seed production. Pyrenochaeta sp. formed lesions on the roots of seedlings, and at high doses delayed flowering and had a negative impact on seed quantity and quality. PMID:23573215

  10. Uptake of Neonicotinoid Insecticides by Water-Foraging Honey Bees (Hymenoptera: Apidae) Through Guttation Fluid of Winter Oilseed Rape.

    PubMed

    Reetz, J E; Schulz, W; Seitz, W; Spiteller, M; Zühlke, S; Armbruster, W; Wallner, K

    2016-02-01

    The water-foraging activity of honey bees (Apis mellifera L.) on guttation fluid of seed-coated crops, such as winter oilseed rape (WOR; Brassica napus L.), has not yet been evaluated. We analyzed the uptake of active substances (a.s.) in guttation fluid by evaluating residues of honey-sac contents. In autumn, insecticide residues of up to 130 µg a.s. per liter were released in WOR guttation fluid; this concentration is noticeably lower than levels reported in guttation fluid of seed-coated maize. Until winter dormancy, the concentrations declined to <30 µg a.s. per liter. In spring, residues were linked to prewintered plants and declined steadily until flowering. The maximum release of residues in guttation fluid of seed-coated WOR occurs on the first leaves in autumn when the colonies' water demand decreases. For the first time, proof for the uptake of guttation fluid from seed-coated WOR by honey bees was provided by measuring residues in individual honey-sac contents. In total, 38 out of 204 samples (19%) showed residues of thiamethoxam at concentrations ranging from 0.3 to 0.95 µg per liter while the corresponding concentrations in guttation fluid of WOR varied between 3.6 to 12.9 µg thiamethoxam per liter. The amounts of thiamethoxam we found in the honey sacs of water-foraging honey bees were therefore below the thresholds in nectar and pollen that are considered to have negative effects on honey bees after chronic exposure. PMID:26516090

  11. Genetic Diversity of Oilseed Rape Fields and Feral Populations in the Context of Coexistence with GM Crops.

    PubMed

    Bailleul, Diane; Ollier, Sébastien; Lecomte, Jane

    2016-01-01

    Despite growing concern about transgenes escaping from fields, few studies have analysed the genetic diversity of crops in an agroecosystem over several years. Accurate information about the dynamics and relationship of the genetic diversity of crops in an agroecosystem is essential for risk assessment and policies concerning the containment of genetically modified crops and their coexistence with crops grown by conventional practices. Here, we analysed the genetic diversity of oilseed rape plants from fields and feral populations over 4 years in an agricultural landscape of 41 km2. We used exact compatibility and maximum likelihood assignment methods to assign these plants to cultivars. Even pure lines and hybrid cultivar seed lots contained several genotypes. The cultivar diversity in fields reflected the conventional view of agroecosystems quite well: that is, there was a succession of cultivars, some grown for longer than others because of their good performance, some used for one year and then abandoned, and others gradually adopted. Three types of field emerged: fields sown with a single cultivar, fields sown with two cultivars, and unassigned fields (too many cultivars or unassigned plants to reliably assign the field). Field plant diversity was higher than expected, indicating the persistence of cultivars that were grown for only one year. The cultivar composition of feral populations was similar to that of field plants, with an increasing number of cultivars each year. By using genetic tools, we found a link between the cultivars of field plants in a particular year and the cultivars of feral population plants in the following year. Feral populations on road verges were more diverse than those on path verges. All of these findings are discussed in terms of their consequences in the context of coexistence with genetically modified crops. PMID:27359342

  12. Genetic Diversity of Oilseed Rape Fields and Feral Populations in the Context of Coexistence with GM Crops

    PubMed Central

    Bailleul, Diane; Ollier, Sébastien; Lecomte, Jane

    2016-01-01

    Despite growing concern about transgenes escaping from fields, few studies have analysed the genetic diversity of crops in an agroecosystem over several years. Accurate information about the dynamics and relationship of the genetic diversity of crops in an agroecosystem is essential for risk assessment and policies concerning the containment of genetically modified crops and their coexistence with crops grown by conventional practices. Here, we analysed the genetic diversity of oilseed rape plants from fields and feral populations over 4 years in an agricultural landscape of 41 km2. We used exact compatibility and maximum likelihood assignment methods to assign these plants to cultivars. Even pure lines and hybrid cultivar seed lots contained several genotypes. The cultivar diversity in fields reflected the conventional view of agroecosystems quite well: that is, there was a succession of cultivars, some grown for longer than others because of their good performance, some used for one year and then abandoned, and others gradually adopted. Three types of field emerged: fields sown with a single cultivar, fields sown with two cultivars, and unassigned fields (too many cultivars or unassigned plants to reliably assign the field). Field plant diversity was higher than expected, indicating the persistence of cultivars that were grown for only one year. The cultivar composition of feral populations was similar to that of field plants, with an increasing number of cultivars each year. By using genetic tools, we found a link between the cultivars of field plants in a particular year and the cultivars of feral population plants in the following year. Feral populations on road verges were more diverse than those on path verges. All of these findings are discussed in terms of their consequences in the context of coexistence with genetically modified crops. PMID:27359342

  13. An endophytic bacterium Acinetobacter calcoaceticus Sasm3-enhanced phytoremediation of nitrate-cadmium compound polluted soil by intercropping Sedum alfredii with oilseed rape.

    PubMed

    Chen, Bao; Ma, Xiaoxiao; Liu, Guiqing; Xu, Xiaomeng; Pan, Fengshan; Zhang, Jie; Tian, Shengke; Feng, Ying; Yang, Xiaoe

    2015-11-01

    Intensive agricultural system with high input of fertilizer results in high agricultural output. However, excessive fertilization in intensive agricultural system has great potential to cause nitrate and heavy metal accumulation in soil, which is adverse to human health. The main objective of the present study was to observe the effects of intercropping and inoculation of endophytic bacterium Acinetobacter calcoaceticus Sasm3 on phytoremediation of combined contaminated soil in oilseed rape (Brassica napus L.). The results showed that with Sasm3 inoculation, the biomass of rape was increased by 10-20% for shoot, 64% for root, and 23-29% for seeds while the nitrate accumulation in rape was decreased by 14% in root and by 12% in shoot. The cadmium concentration in rape increased significantly with mono-inoculating treatment, whereas it decreased significantly after intercropping treatment. By denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR analysis, the diversity of bacterial community and the number of nirS and nirK gene copies increased significantly with inoculation or/and intercropping treatment. In conclusion, the endophytic bacterium Sasm3-inoculated intercropping system not only improved the efficiency of clearing cadmium from soil without obstructing crop production, but also improved the quality of crop. PMID:26146371

  14. Formulations of the endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiorum on oilseed rape and improve plant vigor in field trials conducted at separate locations.

    PubMed

    Hu, Xiaojia; Roberts, Daniel P; Maul, Jude E; Emche, Sarah E; Liao, Xing; Guo, Xuelan; Liu, Yeying; McKenna, Laurie F; Buyer, Jeffrey S; Liu, Shengyi

    2011-07-01

    Sclerotinia sclerotiorum causes serious yield losses in crops in the People's Republic of China. Two formulations of oilseed rape seed containing the bacterium Bacillus subtilis Tu-100 were evaluated for suppression of this pathogen in field trials conducted at two independent locations. The pellet formulation significantly reduced disease (incidence and disease index) and increased plant dry mass, while the wrap formulation significantly reduced disease incidence and significantly increased plant dry mass at both field locations. Mean seed yield per 120 plants with both formulations of isolate Tu-100 was significantly greater than the appropriate controls, but at only one of the locations. Both formulations provided stable B. subtilis Tu-100 biomass (≥10(5) CFU·g(-1)) and seed germination (≥85%) over a 6 month period at room temperature. Polymerase chain reaction and DNA sequence analysis identified ituC and ituD, and bacAB and bacD in the genome of isolate Tu-100. These genes are involved in the biosynthesis of iturin and bacilysin. Iturin was detected in culture filtrates from isolate Tu-100, with thin layer chromatography. Detection of bacilysin was not attempted. Experiments reported here indicate the commercial viability of B. subtilis Tu-100 for suppression of S. sclerotiorum on oilseed rape. PMID:21767217

  15. Nano-TiO2 Is Not Phytotoxic As Revealed by the Oilseed Rape Growth and Photosynthetic Apparatus Ultra-Structural Response.

    PubMed

    Li, Jun; Naeem, Muhammad Shahbaz; Wang, Xiuping; Liu, Lixin; Chen, Chang; Ma, Ni; Zhang, Chunlei

    2015-01-01

    Recently nano-materials are widely used but they have shown contrasting effects on human and plant life. Keeping in view the contrasting results, the present study has evaluated plant growth response, antioxidant system activity and photosynthetic apparatus physiological and ultrastructural changes in Brassica napus L. plants grown under a wide range (0, 500, 2500, 4000 mg/l) of nano-TiO2 in a pot experiment. Nano-TiO2 has significantly improved the morphological and physiological indices of oilseed rape plants under our experimental conditions. All the parameters i-e morphological (root length, plant height, fresh biomass), physiological (photosynthetic gas exchange, chlorophyll content, nitrate reductase activity) and antioxidant system (Superoxide dismutase, SOD; Guaiacol peroxidase, POD; Catalase, CAT) recorded have shown improvement in their performance by following nano-TiO2 dose-dependent manner. No significant chloroplast ultra-structural changes were observed. Transmission electron microscopic images have shown that intact & typical grana and stroma thylakoid membranes were in the chloroplast, which suggest that nano-TiO2 has not induced the stressful environment within chloroplast. Finally, it is suggested that, nano-TiO2 have growth promoting effect on oilseed rape plants. PMID:26624621

  16. Nano-TiO2 Is Not Phytotoxic As Revealed by the Oilseed Rape Growth and Photosynthetic Apparatus Ultra-Structural Response

    PubMed Central

    Wang, Xiuping; Liu, Lixin; Chen, Chang; Ma, Ni; Zhang, Chunlei

    2015-01-01

    Recently nano-materials are widely used but they have shown contrasting effects on human and plant life. Keeping in view the contrasting results, the present study has evaluated plant growth response, antioxidant system activity and photosynthetic apparatus physiological and ultrastructural changes in Brassica napus L. plants grown under a wide range (0, 500, 2500, 4000 mg/l) of nano-TiO2 in a pot experiment. Nano-TiO2 has significantly improved the morphological and physiological indices of oilseed rape plants under our experimental conditions. All the parameters i-e morphological (root length, plant height, fresh biomass), physiological (photosynthetic gas exchange, chlorophyll content, nitrate reductase activity) and antioxidant system (Superoxide dismutase, SOD; Guaiacol peroxidase, POD; Catalase, CAT) recorded have shown improvement in their performance by following nano-TiO2 dose-dependent manner. No significant chloroplast ultra-structural changes were observed. Transmission electron microscopic images have shown that intact & typical grana and stroma thylakoid membranes were in the chloroplast, which suggest that nano-TiO2 has not induced the stressful environment within chloroplast. Finally, it is suggested that, nano-TiO2 have growth promoting effect on oilseed rape plants. PMID:26624621

  17. Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype

    PubMed Central

    Havlícková, Lenka; Jozová, Eva; Klíma, Miroslav; Kucera, Vratislav; Curn, Vladislav

    2014-01-01

    The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these reliable molecular markers are based on discrimination of class I S-locus genes that are present in self-compatible plants, there is a need to use an internal control in order to detect possible PCR inhibition that gives false results during genotyping. In this study, 269 double haploid F2 oilseed rape plants obtained by microspore embryogenesis were used to verify the applicability of an improved PCR assay based on the detection of the class I SLG gene along with an internal control. Comparative analysis of the PCR genotyping results vs. S phenotype analysis confirmed the applicability of this molecular approach in hybrid breeding programs. This approach allows accurate detection of self-incompatible plants via a different amplification profile. PMID:25249779

  18. Antioxidant enzyme systems and the ascorbate-glutathione cycle as contributing factors to cadmium accumulation and tolerance in two oilseed rape cultivars (Brassica napus L.) under moderate cadmium stress.

    PubMed

    Wu, Zhichao; Zhao, Xiaohu; Sun, Xuecheng; Tan, Qiling; Tang, Yafang; Nie, Zhaojun; Qu, Chanjuan; Chen, Zuoxin; Hu, Chengxiao

    2015-11-01

    Oilseed rape (Brassica napus L.) with high tolerance to cadmium (Cd) may be used in the phytoremediation of Cd-contaminated fields. However, the mechanisms responsible for Cd accumulation and tolerance in oilseed rape are still poorly understood. Here, we investigated the physiological and molecular processes involved in Cd tolerance of two oilseed rape cultivars with different Cd accumulation abilities. The total Cd accumulation in cultivar L351 was higher than cultivar L338, particularly with increasing concentrations of Cd exposure. L338 was a more pronounced Cd-sensitive cultivar than L351, while higher activities of antioxidant enzymes (CAT, APX, GR, DHAR) as well as higher contents of GSH and AsA were all observed in L351 under Cd treatments, especially at high levels. No differences were found in SOD activities between the two cultivars under the same Cd treatments, suggesting that SOD was not the key factor in relation to the differences of Cd tolerance and accumulation between them. Gene expression levels of BnFe-SOD, BnCAT, BnAPX, BcGR and BoDHAR in roots of L351 were relatively higher than that in L338 under Cd exposure as well as BnCAT and BcGR in leaves. It is concluded that antioxidant enzymes and the ascorbate-glutathione cycle play important roles in oilseed rape Cd accumulation and tolerance. PMID:26207887

  19. Long-Term Monitoring of Field Trial Sites with Genetically Modified Oilseed Rape (Brassica napus L.) in Saxony-Anhalt, Germany. Fifteen Years Persistence to Date but No Spatial Dispersion

    PubMed Central

    Belter, Anke

    2016-01-01

    Oilseed rape is known to persist in arable fields because of its ability to develop secondary seed dormancy in certain agronomic and environmental conditions. If conditions change, rapeseeds are able to germinate up to 10 years later to build volunteers in ensuing crops. Extrapolations of experimental data acted on the assumption of persistence periods for more than 20 years after last harvest of rapeseed. Genetically-modified oilseed rape—cultivated widely in Northern America since 1996—is assumed not to differ from its conventional form in this property. Here, experimental data are reported from official monitoring activities that verify these assumptions. At two former field trial sites in Saxony-Anhalt genetically-modified herbicide-resistant oilseed rape volunteers are found up to fifteen years after harvest. Nevertheless, spatial dispersion or establishment of GM plants outside of the field sites was not observed within this period. PMID:26784233

  20. Ideotype population exploration: growth, photosynthesis, and yield components at different planting densities in winter oilseed rape (Brassica napus L.).

    PubMed

    Ma, Ni; Yuan, Jinzhan; Li, Ming; Li, Jun; Zhang, Liyan; Liu, Lixin; Naeem, Muhammad Shahbaz; Zhang, Chunlei

    2014-01-01

    Rapeseed is one of the most important edible oil crops in the world and the seed yield has lagged behind the increasing demand driven by population growth. Winter oilseed rape (Brassica napus L.) is widely cultivated with relatively low yield in China, so it is necessary to find the strategies to improve the expression of yield potential. Planting density has great effects on seed yield of crops. Hence, field experiments were conducted in Wuhan in the Yangtze River basin with one conventional variety (Zhongshuang 11, ZS11) and one hybrid variety (Huayouza 9, HYZ9) at five planting densities (27.0×10(4), 37.5×10(4), 48.0×10(4), 58.5×10(4), 69.0×10(4) plants ha(-1)) during 2010-2012 to investigate the yield components. The physiological traits for high-yield and normal-yield populations were measured during 2011-2013. Our results indicated that planting densities of 58.5×10(4) plants ha(-1) in ZS11 and 48.0×10(4) plants ha(-1) in HYZ9 have significantly higher yield compared with the density of 27.0×10(4) plants ha(-1) for both varieties. The ideal silique numbers for ZS11 and HYZ9 were ∼0.9×10(4) (n m(-2)) and ∼1×10(4) (n m(-2)), respectively, and ideal primary branches for ZS11 and HYZ9 were ∼250 (n m(-2)) and ∼300 (n m(-2)), respectively. The highest leaf area index (LAI) and silique wall area index (SAI) was ∼5.0 and 7.0, respectively. Moreover, higher leaf net photosynthetic rate (Pn) and water use efficiency (WUE) were observed in the high-yield populations. A significantly higher level of silique wall photosynthesis and rapid dry matter accumulation were supposed to result in the maximum seed yield. Our results suggest that increasing the planting density within certain range is a feasible approach for higher seed yield in winter rapeseed in China. PMID:25517990

  1. Ideotype Population Exploration: Growth, Photosynthesis, and Yield Components at Different Planting Densities in Winter Oilseed Rape (Brassica napus L.)

    PubMed Central

    Ma, Ni; Yuan, Jinzhan; Li, Ming; Li, Jun; Zhang, Liyan; Liu, Lixin; Naeem, Muhammad Shahbaz; Zhang, Chunlei

    2014-01-01

    Rapeseed is one of the most important edible oil crops in the world and the seed yield has lagged behind the increasing demand driven by population growth. Winter oilseed rape (Brassica napus L.) is widely cultivated with relatively low yield in China, so it is necessary to find the strategies to improve the expression of yield potential. Planting density has great effects on seed yield of crops. Hence, field experiments were conducted in Wuhan in the Yangtze River basin with one conventional variety (Zhongshuang 11, ZS11) and one hybrid variety (Huayouza 9, HYZ9) at five planting densities (27.0×104, 37.5×104, 48.0×104, 58.5×104, 69.0×104 plants ha–1) during 2010–2012 to investigate the yield components. The physiological traits for high-yield and normal-yield populations were measured during 2011–2013. Our results indicated that planting densities of 58.5×104 plants ha–1 in ZS11 and 48.0×104 plants ha–1 in HYZ9 have significantly higher yield compared with the density of 27.0×104 plants ha–1for both varieties. The ideal silique numbers for ZS11 and HYZ9 were ∼0.9×104 (n m–2) and ∼1×104 (n m-2), respectively, and ideal primary branches for ZS11 and HYZ9 were ∼250 (n m–2) and ∼300 (n m–2), respectively. The highest leaf area index (LAI) and silique wall area index (SAI) was ∼5.0 and 7.0, respectively. Moreover, higher leaf net photosynthetic rate (Pn) and water use efficiency (WUE) were observed in the high-yield populations. A significantly higher level of silique wall photosynthesis and rapid dry matter accumulation were supposed to result in the maximum seed yield. Our results suggest that increasing the planting density within certain range is a feasible approach for higher seed yield in winter rapeseed in China. PMID:25517990

  2. [Effect of atmospheric CO2 concentration and nitrogen application level on absorption and transportation of nutrient elements in oilseed rape].

    PubMed

    Wang, Wen-ming; Zhang, Zhen-hua; Song, Hai-xing; Liu, Qiang; Rong, Xiang-min; Guan, Chun-yun; Zeng, Jing; Yuan, Dan

    2015-07-01

    Effect of elevated atmospheric-CO2 (780 µmol . mol-1) on the absorption and transportation of secondary nutrient elements (calcium, magnesium, sulphur) and micronutrient elements (iron, manganese, zinc, molybdenum and boron) in oilseed rape at the stem elongation stage were studied by greenhouse simulated method. Compared with the ambient CO2 condition, the content of Zn in stem was increased and the contents of other nutrient elements were decreased under the elevated atmospheric-CO2 with no nitrogen (N) application; the contents of Ca, S, B and Zn were increased, and the contents of Mg, Mn, Mo and Fe were decreased under the elevated atmospheric CO2 with N application (0.2 g N . kg-1 soil); except the content of Mo in leaf was increased, the contents of other nutrient elements were decreased under the elevated atmospheric-CO2 with two levels of N application. Compared with the ambient CO2 condition, the amounts of Ca and S relative to the total amount of secondary nutrient elements in stem and the amounts of B and Zn relative to the total amount of micronutrient elements in stem were increased under the elevated-CO2 treatment with both levels of N application, and the corresponding values of Mg, Fe, Mn and Mo were decreased; no-N application treatment increased the proportion of Ca distributed into the leaves, and the proportion of Mg distributed into leaves was increased by the normal-N application level; the proportions of Mn, Zn and Mo distributed into the leaves were increased at both N application levels. Without N application, the elevation of atmospheric CO2 increased the transport coefficients of SFe, Mo and SS,B, but decreased the transport coefficients of SMg,Fe, SMg, Mn and SS,Fe, indicating the proportions of Mo, S transported into the upper part of plant tissues was higher than that of Fe, and the corresponding value of B was higher than that observed for S, the corresponding value of Mg was higher than that of Fe and Mn. Under normal-N application

  3. Overexpression of Sinapine Esterase BnSCE3 in Oilseed Rape Seeds Triggers Global Changes in Seed Metabolism1[W][OA

    PubMed Central

    Clauß, Kathleen; von Roepenack-Lahaye, Edda; Böttcher, Christoph; Roth, Mary R.; Welti, Ruth; Erban, Alexander; Kopka, Joachim; Scheel, Dierk; Milkowski, Carsten; Strack, Dieter

    2011-01-01

    Sinapine (O-sinapoylcholine) is the predominant phenolic compound in a complex group of sinapate esters in seeds of oilseed rape (Brassica napus). Sinapine has antinutritive activity and prevents the use of seed protein for food and feed. A strategy was developed to lower its content in seeds by expressing an enzyme that hydrolyzes sinapine in developing rape seeds. During early stages of seedling development, a sinapine esterase (BnSCE3) hydrolyzes sinapine, releasing choline and sinapate. A portion of choline enters the phospholipid metabolism, and sinapate is routed via 1-O-sinapoyl-β-glucose into sinapoylmalate. Transgenic oilseed rape lines were generated expressing BnSCE3 under the control of a seed-specific promoter. Two distinct single-copy transgene insertion lines were isolated and propagated to generate homozygous lines, which were subjected to comprehensive phenotyping. Sinapine levels of transgenic seeds were less than 5% of wild-type levels, whereas choline levels were increased. Weight, size, and water content of transgenic seeds were significantly higher than those of wild-type seeds. Seed quality parameters, such as fiber and glucosinolate levels, and agronomically important traits, such as oil and protein contents, differed only slightly, except that amounts of hemicellulose and cellulose were about 30% higher in transgenic compared with wild-type seeds. Electron microscopic examination revealed that a fraction of the transgenic seeds had morphological alterations, characterized by large cavities near the embryonic tissue. Transgenic seedlings were larger than wild-type seedlings, and young seedlings exhibited longer hypocotyls. Examination of metabolic profiles of transgenic seeds indicated that besides suppression of sinapine accumulation, there were other dramatic differences in primary and secondary metabolism. Mapping of these changes onto metabolic pathways revealed global effects of the transgenic BnSCE3 expression on seed metabolism. PMID

  4. Transforming petals into sepaloid organs in Arabidopsis and oilseed rape: implementation of the hairpin RNA-mediated gene silencing technology in an organ-specific manner.

    PubMed

    Byzova, Marina; Verduyn, Christoph; De Brouwer, Dirk; De Block, Marc

    2004-01-01

    Oilseed rape ( Brassica napus L.) genotypes with no or small petals are thought to have advantages in photosynthetic activity. The flowers of field-grown oilseed rape form a bright-yellow canopy that reflects and absorbs nearly 60% of the photosynthetically active radiation (PAR), causing a severe yield penalty. Reducing the size of the petals and/or removing the reflecting colour will improve the transmission of PAR to the leaves and is expected to increase the crop productivity. In this study the 'hairpin' RNA-mediated (hpRNA) gene silencing technology was implemented in Arabidopsis thaliana (L.) Heynh. and B. napus to silence B-type MADS-box floral organ identity genes in a second-whorl-specific manner. In Arabidopsis, silencing of B-type MADS-box genes was obtained by expressing B. napus APETALA3( BAP3) or PISTILLATA ( BPI) homologous self-complementary hpRNA constructs under control of the Arabidopsis A-type MADS-box gene APETALA1 ( AP1) promoter. In B. napus, silencing of the BPI gene family was achieved by expressing a similar hpRNA construct as used in Arabidopsis under the control of a chimeric promoter consisting of a modified petal-specific Arabidopsis AP3 promoter fragment fused to the AP1 promoter. In this way, transgenic plants were generated producing male fertile flowers in which the petals were converted into sepals ( Arabidopsis) or into sepaloid petals ( B. napus). These novel flower phenotypes were stable and heritable in both species. PMID:14534787

  5. Potential Effects of Oilseed Rape Expressing Oryzacystatin-1 (OC-1) and of Purified Insecticidal Proteins on Larvae of the Solitary Bee Osmia bicornis

    PubMed Central

    Konrad, Roger; Ferry, Natalie; Gatehouse, Angharad M. R.; Babendreier, Dirk

    2008-01-01

    Despite their importance as pollinators in crops and wild plants, solitary bees have not previously been included in non-target testing of insect-resistant transgenic crop plants. Larvae of many solitary bees feed almost exclusively on pollen and thus could be highly exposed to transgene products expressed in the pollen. The potential effects of pollen from oilseed rape expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1) were investigated on larvae of the solitary bee Osmia bicornis ( = O. rufa). Furthermore, recombinant OC-1 (rOC-1), the Bt toxin Cry1Ab and the snowdrop lectin Galanthus nivalis agglutinin (GNA) were evaluated for effects on the life history parameters of this important pollinator. Pollen provisions from transgenic OC-1 oilseed rape did not affect overall development. Similarly, high doses of rOC-1 and Cry1Ab as well as a low dose of GNA failed to cause any significant effects. However, a high dose of GNA (0.1%) in the larval diet resulted in significantly increased development time and reduced efficiency in conversion of pollen food into larval body weight. Our results suggest that OC-1 and Cry1Ab expressing transgenic crops would pose a negligible risk for O. bicornis larvae, whereas GNA expressing plants could cause detrimental effects, but only if bees were exposed to high levels of the protein. The described bioassay with bee brood is not only suitable for early tier non-target tests of transgenic plants, but also has broader applicability to other crop protection products. PMID:18628826

  6. Potential effects of oilseed rape expressing oryzacystatin-1 (OC-1) and of purified insecticidal proteins on larvae of the solitary bee Osmia bicornis.

    PubMed

    Konrad, Roger; Ferry, Natalie; Gatehouse, Angharad M R; Babendreier, Dirk

    2008-01-01

    Despite their importance as pollinators in crops and wild plants, solitary bees have not previously been included in non-target testing of insect-resistant transgenic crop plants. Larvae of many solitary bees feed almost exclusively on pollen and thus could be highly exposed to transgene products expressed in the pollen. The potential effects of pollen from oilseed rape expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1) were investigated on larvae of the solitary bee Osmia bicornis (= O. rufa). Furthermore, recombinant OC-1 (rOC-1), the Bt toxin Cry1Ab and the snowdrop lectin Galanthus nivalis agglutinin (GNA) were evaluated for effects on the life history parameters of this important pollinator. Pollen provisions from transgenic OC-1 oilseed rape did not affect overall development. Similarly, high doses of rOC-1 and Cry1Ab as well as a low dose of GNA failed to cause any significant effects. However, a high dose of GNA (0.1%) in the larval diet resulted in significantly increased development time and reduced efficiency in conversion of pollen food into larval body weight. Our results suggest that OC-1 and Cry1Ab expressing transgenic crops would pose a negligible risk for O. bicornis larvae, whereas GNA expressing plants could cause detrimental effects, but only if bees were exposed to high levels of the protein. The described bioassay with bee brood is not only suitable for early tier non-target tests of transgenic plants, but also has broader applicability to other crop protection products. PMID:18628826

  7. Impact of transgenic oilseed rape expressing oryzacystatin-1 (OC-1) and of insecticidal proteins on longevity and digestive enzymes of the solitary bee Osmia bicornis.

    PubMed

    Konrad, Roger; Connor, Melanie; Ferry, Natalie; Gatehouse, Angharad M R; Babendreier, Dirk

    2009-04-01

    The risk that insect-resistant transgenic plants may pose for solitary bees was assessed by determining longevity of adult Osmia bicornis (O. rufa) chronically exposed to transgenic oilseed rape expressing oryzacystatin-1 (OC-1) or to the purified insecticidal proteins recombinant rOC-1, Kunitz soybean trypsin inhibitor (SBTI), Galanthus nivalis agglutinin (GNA), or Bacillus thuringiensis toxin Cry1Ab dissolved in sugar solution (at 0.01 and 0.1%, w:v, Cry1Ab only at 0.01%). Compared to control bees, longevity was significantly reduced by SBTI and GNA at both concentrations and by rOC-1 at 0.1%, but not by Cry1Ab or rOC-1 at 0.01%. Longevity on the OC-1 oilseed rape was not significantly different from the control plants. The effects of SBTI and rOC-1 on longevity were investigated through characterization of the digestive proteinases of O. bicornis and analysis of the response in proteinase profiles to ingestion of these proteinase inhibitors. A relatively complex profile of at least four types of soluble proteolytic enzymes was identified. Serine proteinases were found to be predominant, with metallo and especially cysteine proteinases making a smaller albeit significant contribution. The compensatory response to in vivo enzyme inhibition was similar for SBTI and rOC-1 although less pronounced for rOC-1. It consisted of a non-specific overproduction of native proteinases, both sensitive and insensitive, and the induction of a novel aspartic proteinase. PMID:19135058

  8. Oilseed rape seeds with ablated defence cells of the glucosinolate–myrosinase system. Production and characteristics of double haploid MINELESS plants of Brassica napus L.

    PubMed Central

    Ahuja, Ishita; Borgen, Birgit Hafeld; Hansen, Magnor; Honne, Bjørn Ivar; Müller, Caroline; Rohloff, Jens; Rossiter, John Trevor; Bones, Atle Magnar

    2011-01-01

    Oilseed rape and other crop plants of the family Brassicaceae contain a unique defence system known as the glucosinolate–myrosinase system or the ‘mustard oil bomb’. The ‘mustard oil bomb’ which includes myrosinase and glucosinolates is triggered by abiotic and biotic stress, resulting in the formation of toxic products such as nitriles and isothiocyanates. Myrosinase is present in specialist cells known as ‘myrosin cells’ and can also be known as toxic mines. The myrosin cell idioblasts of Brassica napus were genetically reprogrammed to undergo controlled cell death (ablation) during seed development. These myrosin cell-free plants have been named MINELESS as they lack toxic mines. This has led to the production of oilseed rape with a significant reduction both in myrosinase levels and in the hydrolysis of glucosinolates. Even though the myrosinase activity in MINELESS was very low compared with the wild type, variation was observed. This variability was overcome by producing homozygous seeds. A microspore culture technique involving non-fertile haploid MINELESS plants was developed and these plants were treated with colchicine to produce double haploid MINELESS plants with full fertility. Double haploid MINELESS plants had significantly reduced myrosinase levels and glucosinolate hydrolysis products. Wild-type and MINELESS plants exhibited significant differences in growth parameters such as plant height, leaf traits, matter accumulation, and yield parameters. The growth and developmental pattern of MINELESS plants was relatively slow compared with the wild type. The characteristics of the pure double haploid MINELESS plant are described and its importance for future biochemical, agricultural, dietary, functional genomics, and plant defence studies is discussed. PMID:21778185

  9. An Individual-Based Model of the Evolution of Pesticide Resistance in Heterogeneous Environments: Control of Meligethes aeneus Population in Oilseed Rape Crops

    PubMed Central

    Stratonovitch, Pierre; Elias, Jan; Denholm, Ian; Slater, Russell; Semenov, Mikhail A.

    2014-01-01

    Preventing a pest population from damaging an agricultural crop and, at the same time, preventing the development of pesticide resistance is a major challenge in crop protection. Understanding how farming practices and environmental factors interact with pest characteristics to influence the spread of resistance is a difficult and complex task. It is extremely challenging to investigate such interactions experimentally at realistic spatial and temporal scales. Mathematical modelling and computer simulation have, therefore, been used to analyse resistance evolution and to evaluate potential resistance management tactics. Of the many modelling approaches available, individual-based modelling of a pest population offers most flexibility to include and analyse numerous factors and their interactions. Here, a pollen beetle (Meligethes aeneus) population was modelled as an aggregate of individual insects inhabiting a spatially heterogeneous landscape. The development of the pest and host crop (oilseed rape) was driven by climatic variables. The agricultural land of the landscape was managed by farmers applying a specific rotation and crop protection strategy. The evolution of a single resistance allele to the pyrethroid lambda cyhalothrin was analysed for different combinations of crop management practices and for a recessive, intermediate and dominant resistance allele. While the spread of a recessive resistance allele was severely constrained, intermediate or dominant resistance alleles showed a similar response to the management regime imposed. Calendar treatments applied irrespective of pest density accelerated the development of resistance compared to ones applied in response to prescribed pest density thresholds. A greater proportion of spring-sown oilseed rape was also found to increase the speed of resistance as it increased the period of insecticide exposure. Our study demonstrates the flexibility and power of an individual-based model to simulate how farming

  10. Effects on weed and invertebrate abundance and diversity of herbicide management in genetically modified herbicide-tolerant winter-sown oilseed rape

    PubMed Central

    Bohan, David A; Boffey, Caroline W.H; Brooks, David R; Clark, Suzanne J; Dewar, Alan M; Firbank, Les G; Haughton, Alison J; Hawes, Cathy; Heard, Matthew S; May, Mike J; Osborne, Juliet L; Perry, Joe N; Rothery, Peter; Roy, David B; Scott, Rod J; Squire, Geoff R; Woiwod, Ian P; Champion, Gillian T

    2005-01-01

    We evaluated the effects of the herbicide management associated with genetically modified herbicide-tolerant (GMHT) winter oilseed rape (WOSR) on weed and invertebrate abundance and diversity by testing the null hypothesis that there is no difference between the effects of herbicide management of GMHT WOSR and that of comparable conventional varieties. For total weeds there were few treatment differences between GMHT and conventional cropping, but large and opposite treatment effects were observed for dicots and monocots. In the GMHT treatment, there were fewer dicots and more monocots than in conventional crops. At harvest, dicot biomass and seed rain in the GMHT treatment were one-third of that in the conventional, while monocot biomass was threefold greater and monocot seed rain almost fivefold greater in the GMHT treatment than in the conventional. These differential effects persisted into the following two years of the rotation. Bees and butterflies that forage and select for dicot weeds were less abundant in GMHT WOSR management in July. Year totals for Collembola were greater under GMHT management. There were few other treatment effects on invertebrates, despite the marked effects of herbicide management on the weeds. PMID:15799941

  11. Is the remobilization of S and N reserves for seed filling of winter oilseed rape modulated by sulphate restrictions occurring at different growth stages?

    PubMed Central

    Dubousset, L.; Etienne, P.; Avice, J. C.

    2010-01-01

    How the remobilization of S and N reserves can meet the needs of seeds of oilseed rape subject to limitation of S fertilization remains largely unclear. Thus, this survey aims to determine the incidence of sulphate restriction [low S (LS)] applied at bolting [growth stage (GS) 32], visible bud (GS 53), and start of pod filling (GS 70) on source–sink relationships for S and N, and on the dynamics of endogenous/exogenous S and N contributing to seed yield and quality. Sulphate restrictions applied at GS 32, GS 53, and GS 70 were annotated LS32, LS53, and LS70. Long-term 34SO42− and 15NO3− labelling was used to explore S and N partitioning at the whole-plant level. In LS53, the sulphur remobilization efficiency (SRE) to seeds increased, but not enough to maintain seed quality. In LS32, an early S remobilization from leaves provided S for root, stem, and pod growth, but the subsequent demand for seed development was not met adequately and the N utilization efficiency (NUtE) was reduced when compared with high S (HS). The highest SRE (65±1.2% of the remobilized S) associated with an efficient foliar S mobilization (with minimal residual S concentrations of 0.1–0.2% dry matter) was observed under LS70 treatment, which did not affect yield components. PMID:20693411

  12. Structural Changes in Senescing Oilseed Rape Leaves at Tissue and Subcellular Levels Monitored by Nuclear Magnetic Resonance Relaxometry through Water Status

    PubMed Central

    Musse, Maja; De Franceschi, Loriane; Cambert, Mireille; Sorin, Clément; Le Caherec, Françoise; Burel, Agnès; Bouchereau, Alain; Mariette, François; Leport, Laurent

    2013-01-01

    Nitrogen use efficiency is relatively low in oilseed rape (Brassica napus) due to weak nitrogen remobilization during leaf senescence. Monitoring the kinetics of water distribution associated with the reorganization of cell structures, therefore, would be valuable to improve the characterization of nutrient recycling in leaf tissues and the associated senescence processes. In this study, nuclear magnetic resonance (NMR) relaxometry was used to describe water distribution and status at the cellular level in different leaf ranks of well-watered plants. It was shown to be able to detect slight variations in the evolution of senescence. The NMR results were linked to physiological characterization of the leaves and to light and electron micrographs. A relationship between cell hydration and leaf senescence was revealed and associated with changes in the NMR signal. The relative intensities and the transverse relaxation times of the NMR signal components associated with vacuole water were positively correlated with senescence, describing water uptake and vacuole and cell enlargement. Moreover, the relative intensity of the NMR signal that we assigned to the chloroplast water decreased during the senescence process, in agreement with the decrease in relative chloroplast volume estimated from micrographs. The results are discussed on the basis of water flux occurring at the cellular level during senescence. One of the main applications of this study would be for plant phenotyping, especially for plants under environmental stress such as nitrogen starvation. PMID:23903438

  13. Alleviation of Lead Toxicity by 5-Aminolevulinic Acid Is Related to Elevated Growth, Photosynthesis, and Suppressed Ultrastructural Damages in Oilseed Rape

    PubMed Central

    Tian, Tian; Qin, Yebo; Gill, Rafaqat A.; Ali, Shafaqat

    2014-01-01

    Lead (Pb) is a widely spread pollutant and leads to diverse morphological and structural changes in the plants. In this study, alleviating role of 5-aminolevulinic acid (ALA) in oilseed rape (Brassica napus L.) was investigated with or without foliar application of ALA (25 mg L−1) in hydroponic environment under different Pb levels (0, 100, and 400 µM). Outcomes stated that plant morphology and photosynthetic attributes were reduced under the application of Pb alone. However, ALA application significantly increased the plant growth and photosynthetic parameters under Pb toxicity. Moreover, ALA also lowered the Pb concentration in shoots and roots under Pb toxicity. The microscopic studies depicted that exogenously applied ALA ameliorated the Pb stress and significantly improved the cell ultrastructures. After application of ALA under Pb stress, mesophyll cell had well-developed nucleus and chloroplast having a number of starch granules. Moreover, micrographs illustrated that root tip cell contained well-developed nucleus, a number of mitochondria, and golgi bodies. These results proposed that under 15-day Pb-induced stress, ALA improved the plant growth, chlorophyll content, photosynthetic parameters, and ultrastructural modifications in leaf mesophyll and root tip cells of the B. napus plants. PMID:24683549

  14. A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement.

    PubMed

    Girondé, Alexandra; Poret, Marine; Etienne, Philippe; Trouverie, Jacques; Bouchereau, Alain; Le Cahérec, Françoise; Leport, Laurent; Niogret, Marie-Françoise; Avice, Jean-Christophe

    2015-01-01

    Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein) were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding. PMID:27135221

  15. Effects on weed and invertebrate abundance and diversity of herbicide management in genetically modified herbicide-tolerant winter-sown oilseed rape.

    PubMed

    Bohan, David A; Boffey, Caroline W H; Brooks, David R; Clark, Suzanne J; Dewar, Alan M; Firbank, Les G; Haughton, Alison J; Hawes, Cathy; Heard, Matthew S; May, Mike J; Osborne, Juliet L; Perry, Joe N; Rothery, Peter; Roy, David B; Scott, Rod J; Squire, Geoff R; Woiwod, Ian P; Champion, Gillian T

    2005-03-01

    We evaluated the effects of the herbicide management associated with genetically modified herbicide-tolerant (GMHT) winter oilseed rape (WOSR) on weed and invertebrate abundance and diversity by testing the null hypotheses that there is no difference between the effects of herbicide management of GMHT WOSR and that of comparable conventional varieties. For total weeds, there were few treatment differences between GMHT and conventional cropping, but large and opposite treatment effects were observed for dicots and monocots. In the GMHT treatment, there were fewer dicots and monocots than in conventional crops. At harvest, dicot biomass and seed rain in the GMHT treatment were one-third of that in the conventional, while monocot biomass was threefold greater and monocot seed rain almost fivefold greater in the GMHT treatment than in the conventional. These differential effects persisted into the following two years of the rotation. Bees and Butterflies that forage and select for dicot weeds were less abundant in GMHT WORS management in July. Year totals for Collembola were greater under GMHT management. There were few other treatment effects on invertebrates, despite the marked effects of herbicide management on the weeds. PMID:15799941

  16. A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement

    PubMed Central

    Girondé, Alexandra; Poret, Marine; Etienne, Philippe; Trouverie, Jacques; Bouchereau, Alain; Le Cahérec, Françoise; Leport, Laurent; Niogret, Marie-Françoise; Avice, Jean-Christophe

    2015-01-01

    Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein) were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding. PMID:27135221

  17. The effects of the presence of Bt-transgenic oilseed rape in wild mustard populations on the rhizosphere nematode and microbial communities.

    PubMed

    Liu, Yongbo; Li, Junsheng; Stewart, C Neal; Luo, Zunlan; Xiao, Nengwen

    2015-10-15

    The adventitious presence of transgenic crops in wild plant populations is of ecological and regulatory concern. In this context, their effects on non-target, below-ground organisms are not well understood. Here, we introduced, at various frequencies, Bt-transgenic oilseed rape (OSR, Brassica napus) into wild mustard (Brassica juncea) populations in the presence and absence of the target herbivore (Plutella xylostella). The impacts on soil nematode and microbial communities were assessed in this system. There were no significant changes on the number of nematode genera and abundance in proportions of OSR with mustard. Nonetheless, the Shannon-Wiener and Pielou evenness index was lowest in plant stands containing 50% of Bt-transgenic OSR. Among treatments, there was no significant variation for culturable soil microbes. There was a positive association between foliar herbivory and the abundance of plant parasitic (PP) and cp-3 nematodes, whereas there was no association between herbivory and soil microbial populations. There was no direct effects of the presence of Bt-transgenic OSR in wild mustard populations on the rhizosphere nematode and microbial communities, whereas its indirect effects via aboveground herbivory might be important to consider for biosafety assessments. PMID:26047860

  18. Metabolic network reconstruction and flux variability analysis of storage synthesis in developing oilseed rape (Brassica napus L.) embryos

    SciTech Connect

    Hay, J.; Schwender, J.

    2011-08-01

    Computational simulation of large-scale biochemical networks can be used to analyze and predict the metabolic behavior of an organism, such as a developing seed. Based on the biochemical literature, pathways databases and decision rules defining reaction directionality we reconstructed bna572, a stoichiometric metabolic network model representing Brassica napus seed storage metabolism. In the highly compartmentalized network about 25% of the 572 reactions are transport reactions interconnecting nine subcellular compartments and the environment. According to known physiological capabilities of developing B. napus embryos, four nutritional conditions were defined to simulate heterotrophy or photoheterotrophy, each in combination with the availability of inorganic nitrogen (ammonia, nitrate) or amino acids as nitrogen sources. Based on mathematical linear optimization the optimal solution space was comprehensively explored by flux variability analysis, thereby identifying for each reaction the range of flux values allowable under optimality. The range and variability of flux values was then categorized into flux variability types. Across the four nutritional conditions, approximately 13% of the reactions have variable flux values and 10-11% are substitutable (can be inactive), both indicating metabolic redundancy given, for example, by isoenzymes, subcellular compartmentalization or the presence of alternative pathways. About one-third of the reactions are never used and are associated with pathways that are suboptimal for storage synthesis. Fifty-seven reactions change flux variability type among the different nutritional conditions, indicating their function in metabolic adjustments. This predictive modeling framework allows analysis and quantitative exploration of storage metabolism of a developing B. napus oilseed.

  19. Potential of genetically modified oilseed rape for biofuels in Austria: Land use patterns and coexistence constraints could decrease domestic feedstock production

    PubMed Central

    Moser, Dietmar; Eckerstorfer, Michael; Pascher, Kathrin; Essl, Franz; Zulka, Klaus Peter

    2013-01-01

    Like other EU Member States, Austria will meet the substitution target of the EU European Renewable Energy Directive for transportation almost exclusively by first generation biofuels, primarily biodiesel from oilseed rape (OSR). Genetically modified (GM) plants have been promoted as a new option for biofuel production as they promise higher yield or higher quality feedstock. We tested implications of GM OSR application for biodiesel production in Austria by means of high resolution spatially explicit simulation of 140 different coexistence scenarios within six main OSR cropping regions in Austria (2400 km2). We identified structural land use characteristics such as field size, land use diversity, land holding patterns and the proportion of the target crop as the predominant factors which influence overall production of OSR in a coexistence scenario. Assuming isolation distances of 800 m and non-GM-OSR proportions of at least 10% resulted in a loss of area for cultivation of OSR in all study areas ranging from −4.5% to more than −25%, depending on the percentage of GM farmers and on the region. We could show that particularly the current primary OSR cropping regions are largely unsuitable for coexistence and would suffer from a net loss of OSR area even at isolation distances of 400 or 800 m. Coexistence constraints associated with application of GM OSR are likely to offset possible GM gains by substantially reducing farmland for OSR cultivation, thus contradicting the political aim to increase domestic OSR area to meet the combined demands of food, feed and biofuel production. PMID:26109750

  20. Genome Wide Identification of the Immunophilin Gene Family in Leptosphaeria maculans: A Causal Agent of Blackleg Disease in Oilseed Rape (Brassica napus)

    PubMed Central

    Zouhar, Miloslav; Mazakova, Jana; Rysanek, Pavel

    2014-01-01

    Abstract Phoma stem canker (blackleg) is a disease of world-wide importance on oilseed rape (Brassica napus) and can cause serious losses for crops globally. The disease is caused by dothideomycetous fungus, Leptosphaeria maculans, which is highly virulent/aggressive. Cyclophilins (CYPs) and FK506-binding proteins (FKBPs) are ubiquitous proteins belonging to the peptidyl-prolyl cis/trans isomerase (PPIase) family. They are collectively referred to as immunophilins (IMMs). In the present study, IMM genes, CYP and FKBP in haploid strain v23.1.3 of L. maculans genome, were identified and classified. Twelve CYPs and five FKBPs were determined in total. Domain architecture analysis revealed the presence of a conserved cyclophilin-like domain (CLD) in the case of CYPs and FKBP_C in the case of FKBPs. Interestingly, IMMs in L. maculans also subgrouped into single domain (SD) and multidomain (MD) proteins. They were primarily found to be localized in cytoplasm, nuclei, and mitochondria. Homologous and orthologous gene pairs were also determined by comparison with the model organism Saccharomyces cerevisiae. Remarkably, IMMs of L. maculans contain shorter introns in comparison to exons. Moreover, CYPs, in contrast with FKBPs, contain few exons. However, two CYPs were determined as being intronless. The expression profile of IMMs in both mycelium and infected primary leaves of B. napus demonstrated their potential role during infection. Secondary structure analysis revealed the presence of atypical eight β strands and two α helices fold architecture. Gene ontology analysis of IMMs predicted their significant role in protein folding and PPIase activity. Taken together, our findings for the first time present new prospects of this highly conserved gene family in phytopathogenic fungus. PMID:25259854

  1. Differences between winter oilseed rape (Brassica napus L.) cultivars in nitrogen starvation-induced leaf senescence are governed by leaf-inherent rather than root-derived signals.

    PubMed

    Koeslin-Findeklee, Fabian; Becker, Martin A; van der Graaff, Eric; Roitsch, Thomas; Horst, Walter J

    2015-07-01

    Nitrogen (N) efficiency of winter oilseed rape (Brassica napus L.) line-cultivars (cvs.), defined as high grain yield under N limitation, has been primarily attributed to maintained N uptake during reproductive growth (N uptake efficiency) in combination with delayed senescence of the older leaves accompanied with maintained photosynthetic capacity (functional stay-green). However, it is not clear whether genotypic variation in N starvation-induced leaf senescence is due to leaf-inherent factors and/or governed by root-mediated signals. Therefore, the N-efficient and stay-green cvs. NPZ-1 and Apex were reciprocally grafted with the N-inefficient and early-senescing cvs. NPZ-2 and Capitol, respectively and grown in hydroponics. The senescence status of older leaves after 12 days of N starvation assessed by SPAD, photosynthesis and the expression of the senescence-specific cysteine protease gene SAG12-1 revealed that the stay-green phenotype of the cvs. NPZ-1 and Apex under N starvation was primarily under the control of leaf-inherent factors. The same four cultivars were submitted to N starvation for up to 12 days in a time-course experiment. The specific leaf contents of biologically active and inactive cytokinins (CKs) and the expression of genes involved in CK homeostasis revealed that under N starvation leaves of early-senescing cultivars were characterized by inactivation of biologically active CKs, whereas in stay-green cultivars synthesis, activation, binding of and response to biologically active CKs were favoured. These results suggest that the homeostasis of biologically active CKs was the predominant leaf-inherent factor for cultivar differences in N starvation-induced leaf senescence and thus N efficiency. PMID:25944925

  2. Differences between winter oilseed rape (Brassica napus L.) cultivars in nitrogen starvation-induced leaf senescence are governed by leaf-inherent rather than root-derived signals

    PubMed Central

    Koeslin-Findeklee, Fabian; Becker, Martin A.; van der Graaff, Eric; Roitsch, Thomas; Horst, Walter J.

    2015-01-01

    Nitrogen (N) efficiency of winter oilseed rape (Brassica napus L.) line-cultivars (cvs.), defined as high grain yield under N limitation, has been primarily attributed to maintained N uptake during reproductive growth (N uptake efficiency) in combination with delayed senescence of the older leaves accompanied with maintained photosynthetic capacity (functional stay-green). However, it is not clear whether genotypic variation in N starvation-induced leaf senescence is due to leaf-inherent factors and/or governed by root-mediated signals. Therefore, the N-efficient and stay-green cvs. NPZ-1 and Apex were reciprocally grafted with the N-inefficient and early-senescing cvs. NPZ-2 and Capitol, respectively and grown in hydroponics. The senescence status of older leaves after 12 days of N starvation assessed by SPAD, photosynthesis and the expression of the senescence-specific cysteine protease gene SAG12-1 revealed that the stay-green phenotype of the cvs. NPZ-1 and Apex under N starvation was primarily under the control of leaf-inherent factors. The same four cultivars were submitted to N starvation for up to 12 days in a time-course experiment. The specific leaf contents of biologically active and inactive cytokinins (CKs) and the expression of genes involved in CK homeostasis revealed that under N starvation leaves of early-senescing cultivars were characterized by inactivation of biologically active CKs, whereas in stay-green cultivars synthesis, activation, binding of and response to biologically active CKs were favoured. These results suggest that the homeostasis of biologically active CKs was the predominant leaf-inherent factor for cultivar differences in N starvation-induced leaf senescence and thus N efficiency. PMID:25944925

  3. Genome wide identification of the immunophilin gene family in Leptosphaeria maculans: a causal agent of Blackleg disease in Oilseed Rape (Brassica napus).

    PubMed

    Singh, Khushwant; Zouhar, Miloslav; Mazakova, Jana; Rysanek, Pavel

    2014-10-01

    Abstract Phoma stem canker (blackleg) is a disease of world-wide importance on oilseed rape (Brassica napus) and can cause serious losses for crops globally. The disease is caused by dothideomycetous fungus, Leptosphaeria maculans, which is highly virulent/aggressive. Cyclophilins (CYPs) and FK506-binding proteins (FKBPs) are ubiquitous proteins belonging to the peptidyl-prolyl cis/trans isomerase (PPIase) family. They are collectively referred to as immunophilins (IMMs). In the present study, IMM genes, CYP and FKBP in haploid strain v23.1.3 of L. maculans genome, were identified and classified. Twelve CYPs and five FKBPs were determined in total. Domain architecture analysis revealed the presence of a conserved cyclophilin-like domain (CLD) in the case of CYPs and FKBP_C in the case of FKBPs. Interestingly, IMMs in L. maculans also subgrouped into single domain (SD) and multidomain (MD) proteins. They were primarily found to be localized in cytoplasm, nuclei, and mitochondria. Homologous and orthologous gene pairs were also determined by comparison with the model organism Saccharomyces cerevisiae. Remarkably, IMMs of L. maculans contain shorter introns in comparison to exons. Moreover, CYPs, in contrast with FKBPs, contain few exons. However, two CYPs were determined as being intronless. The expression profile of IMMs in both mycelium and infected primary leaves of B. napus demonstrated their potential role during infection. Secondary structure analysis revealed the presence of atypical eight β strands and two α helices fold architecture. Gene ontology analysis of IMMs predicted their significant role in protein folding and PPIase activity. Taken together, our findings for the first time present new prospects of this highly conserved gene family in phytopathogenic fungus. PMID:25259854

  4. Identification and characterization of microRNAs in oilseed rape (Brassica napus) responsive to infection with the pathogenic fungus Verticillium longisporum using Brassica AA (Brassica rapa) and CC (Brassica oleracea) as reference genomes.

    PubMed

    Shen, Dan; Suhrkamp, Ina; Wang, Yu; Liu, Shenyi; Menkhaus, Jan; Verreet, Joseph-Alexander; Fan, Longjiang; Cai, Daguang

    2014-11-01

    Verticillium longisporum, a soil-borne pathogenic fungus, causes vascular disease in oilseed rape (Brassica napus). We proposed that plant microRNAs (miRNAs) are involved in the plant-V. longisporum interaction. To identify oilseed rape miRNAs, we deep-sequenced two small RNA libraries made from V. longisporum infected/noninfected roots and employed Brassica rapa and Brassica oleracea genomes as references for miRNA prediction and characterization. We identified 893 B. napus miRNAs representing 360 conserved and 533 novel miRNAs, and mapped 429 and 464 miRNAs to the AA and CC genomes, respectively. Microsynteny analysis with the conserved miRNAs and their flanking protein coding sequences revealed 137 AA-CC genome syntenic miRNA pairs and 61 AA and 42 CC genome-unique miRNAs. Sixty-two miRNAs were responsive to the V. longisporum infection. We present data for specific interactions and simultaneously reciprocal changes in the expression levels of the miRNAs and their targets in the infected roots. We demonstrate that miRNAs are involved in the plant-fungus interaction and that miRNA168-Argonaute 1 (AGO1) expression modulation might act as a key regulatory module in a compatible plant-V. longisporum interaction. Our results suggest that V. longisporum may have evolved a virulence mechanism by interference with plant miRNAs to reprogram plant gene expression and achieve infection. PMID:25132374

  5. Heat stress during seed filling interferes with sulfur restriction on grain composition and seed germination in oilseed rape (Brassica napus L.)

    PubMed Central

    Brunel-Muguet, Sophie; D'Hooghe, Philippe; Bataillé, Marie-Paule; Larré, Colette; Kim, Tae-Hwan; Trouverie, Jacques; Avice, Jean-Christophe; Etienne, Philippe; Dürr, Carolyne

    2015-01-01

    In coming decades, increasing temperatures are expected to impact crop yield and seed quality. To develop low input systems, the effects of temperature and sulfur (S) nutrition in oilseed rape, a high S demanding crop, need to be jointly considered. In this study, we investigated the effects of temperatures [High Temperature (HT), 33°C/day, 19°C/night vs. Control Temperature (Ctrl T), 20°C/day, 15°C/day] and S supply [High S (HS), 500 μm SO2−4 vs. Low S (LS), 8.7 μM SO2−4] during seed filling on (i) yield components [seed number, seed dry weight (SDW) and seed yield], (ii) grain composition [nitrogen (N) and S contents] and quality [fatty acid (FA) composition and seed storage protein (SSP) accumulation] and (iii) germination characteristics (pre-harvest sprouting, germination rates and abnormal seedlings). Abscisic acid (ABA), soluble sugar contents and seed conductivity were also measured. HT and LS decreased the number of seeds per plant. SDW was less affected due to compensatory effects since the number of seeds decreased under stress conditions. While LS had negative effects on seed composition by reducing the FA contents and increasing the ratio S-poor SSPs (12S globulins)/S-rich SSPs (2S albumins) ratio, HT had positive effects by increasing S and FA contents and decreasing the C18:2/C18:3 ratio and the 12S/2S protein ratio. Seeds produced under HT showed high pre-harvest sprouting rates along with decreased ABA contents and high rates of abnormal seedlings. HT and LS restriction significantly accelerated germination times. High conductivity, which indicates poor seed storage capacity, was higher in HT seeds. Consistently, the lower ratio of (raffinose + stachyose)/sucrose in HT seeds indicated low seed storage capacity. We demonstrated the effects of HT and LS on grain and on germination characteristics. These results suggest that hormonal changes might control several seed characteristics simultaneously. PMID:25914702

  6. Heat stress during seed filling interferes with sulfur restriction on grain composition and seed germination in oilseed rape (Brassica napus L.).

    PubMed

    Brunel-Muguet, Sophie; D'Hooghe, Philippe; Bataillé, Marie-Paule; Larré, Colette; Kim, Tae-Hwan; Trouverie, Jacques; Avice, Jean-Christophe; Etienne, Philippe; Dürr, Carolyne

    2015-01-01

    In coming decades, increasing temperatures are expected to impact crop yield and seed quality. To develop low input systems, the effects of temperature and sulfur (S) nutrition in oilseed rape, a high S demanding crop, need to be jointly considered. In this study, we investigated the effects of temperatures [High Temperature (HT), 33°C/day, 19°C/night vs. Control Temperature (Ctrl T), 20°C/day, 15°C/day] and S supply [High S (HS), 500 μm SO(2-) 4 vs. Low S (LS), 8.7 μM SO(2-) 4] during seed filling on (i) yield components [seed number, seed dry weight (SDW) and seed yield], (ii) grain composition [nitrogen (N) and S contents] and quality [fatty acid (FA) composition and seed storage protein (SSP) accumulation] and (iii) germination characteristics (pre-harvest sprouting, germination rates and abnormal seedlings). Abscisic acid (ABA), soluble sugar contents and seed conductivity were also measured. HT and LS decreased the number of seeds per plant. SDW was less affected due to compensatory effects since the number of seeds decreased under stress conditions. While LS had negative effects on seed composition by reducing the FA contents and increasing the ratio S-poor SSPs (12S globulins)/S-rich SSPs (2S albumins) ratio, HT had positive effects by increasing S and FA contents and decreasing the C18:2/C18:3 ratio and the 12S/2S protein ratio. Seeds produced under HT showed high pre-harvest sprouting rates along with decreased ABA contents and high rates of abnormal seedlings. HT and LS restriction significantly accelerated germination times. High conductivity, which indicates poor seed storage capacity, was higher in HT seeds. Consistently, the lower ratio of (raffinose + stachyose)/sucrose in HT seeds indicated low seed storage capacity. We demonstrated the effects of HT and LS on grain and on germination characteristics. These results suggest that hormonal changes might control several seed characteristics simultaneously. PMID:25914702

  7. Molecular screening for avirulence alleles AvrLm1 and AvrLm6 in airborne inoculum of Leptosphaeria maculans and winter oilseed rape (Brassica napus) plants from Poland and the UK.

    PubMed

    Kaczmarek, Joanna; Latunde-Dada, Akinwunmi O; Irzykowski, Witold; Cools, Hans J; Stonard, Jenna F; Brachaczek, Andrzej; Jedryczka, Malgorzata

    2014-11-01

    A combination of staining, light microscopy and SYBR green- and dual-labelled fluorescent probe-based qPCR chemistries with species- and gene-specific primers was employed to evaluate fluctuations in the aerial biomass of Leptosphaeria maculans spores captured by volumetric spore trappings in Poznan, Poland (2006, 2008) and Harpenden, UK (2002, 2006). Arising from these surveys, DNA samples extracted from Burkard spore-trap tapes were screened for fluctuation patterns in the frequencies of AvrLm1 and AvrLm6, the most prominent of the 15 genes that code for avirulence effectors in this Dothideomycete cause of the destructive phoma stem canker disease of oilseed rape worldwide. In Poznan, very low frequencies of AvrLm1 allele were found in the autumn of both 2006 and 2008, reflecting significantly increased cultivation of rape seed with Rlm1-based resistance. In contrast, at least six folds-higher frequencies of AvrLm6, which were also confirmed by end-point PCR bioassays on phoma-infected leaves from the same region of Poland, were obtained during both years. In the UK, however, relatively higher AvrLm1 allele titres were found in L. maculans spores captured in air samples from the autumn of 2002 on the experimental fields of Rothamsted Research, Harpenden, that were historically sown to genetically heterogeneous B. napus cultivars. In the 2006 screen these levels had plummeted, to a 1:4 ratio, in favour of frequencies of the AvrLm6 allele. Patterns of fluctuations in erg11 (CYP51) fragments coding for sterol 14α-demethylase suggest October as the month with the most viable wind-dispersed L. maculans propagules of each season of the screens. PMID:25081837

  8. Stability of transgene integration and expression in subsequent generations of doubled haploid oilseed rape transformed with chitinase and beta-1,3-glucanase genes in a double-gene construct.

    PubMed

    Melander, Margareta; Kamnert, Iréne; Happstadius, Ingrid; Liljeroth, Erland; Bryngelsson, Tomas

    2006-09-01

    A double-gene construct with one chitinase and one beta-1,3-glucanase gene from barley, both driven by enhanced 35S promoters, was transformed into oilseed rape. From six primary transformants expressing both transgenes 10 doubled haploid lines were produced and studied for five generations. The number of inserted copies for both the genes was determined by Southern blotting and real-time PCR with full agreement between the two methods. When copy numbers were analysed in different generations, discrepancies were found, indicating that at least part of the inserted sequences were lost in one of the alleles of some doubled haploids. Chitinase and beta-1,3-glucanase expression was analysed by Western blotting in all five doubled haploid generations. Despite that both the genes were present on the same T-DNA and directed by the same promoter their expression pattern between generations was different. The beta-1,3-glucanase was expressed at high and stable levels in all generations, while the chitinase displayed lower expression that varied between generations. The transgenic plants did not show any major impact on fungal resistance when assayed in greenhouse, although purified beta-1,3-glucanase and chitinase caused retardment of fungal growth in vitro. PMID:16565860

  9. A systemic increase in the recombination frequency upon local infection of Arabidopsis thaliana plants with oilseed rape mosaic virus depends on plant age, the initial inoculum concentration and the time for virus replication

    PubMed Central

    Yao, Youli; Kathiria, Palak; Kovalchuk, Igor

    2013-01-01

    In the past, we showed that local infection of tobacco leaves with either tobacco mosaic virus or oilseed rape mosaic virus (ORMV) resulted in a systemic increase in the homologous recombination frequency (HRF). Later on, we showed that a similar phenomenon occurs in Arabidopsis thaliana plants infected with ORMV. Here, we tested whether the time of removing the infected leaves as well as viral titer have any effect on the degree of changes in HRF in systemic tissues. An increase in HRF in systemic non-infected tissues was more pronounced when the infected leaves were detached from the infected plants at 60–96 h post-infection, rather than at earlier time. Next, we found that exposure to higher concentrations of inoculum was much more efficient in triggering an increase in HRF than exposure to lower concentrations. Finally, we showed that older plants exhibited a higher increase in HRF than younger plants. We found that an increase in genome instability in systemic tissues of locally infected plants depends on plant age, the concentration of initial inoculums and the time of viral replication. PMID:23519399

  10. Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.).

    PubMed

    Zhu, Yana; Cao, Zhengying; Xu, Fei; Huang, Yi; Chen, Mingxun; Guo, Wanli; Zhou, Weijun; Zhu, Jun; Meng, Jinling; Zou, Jitao; Jiang, Lixi

    2012-02-01

    Seed oil production in oilseed rape is greatly affected by the temperature during seed maturation. However, the molecular mechanism of the interaction between genotype and temperature in seed maturation remains largely unknown. We developed two near-isogenic lines (NIL-9 and NIL-1), differing mainly at a QTL region influencing oil content on Brassica napus chromosome C2 (qOC.C2.2) under high temperature during seed maturation. The NILs were treated under different temperatures in a growth chamber after flowering. RNA from developing seeds was extracted on the 25th day after flowering (DAF), and transcriptomes were determined by microarray analysis. Statistical analysis indicated that genotype, temperature, and the interaction between genotype and temperature (G × T) all significantly affected the expression of the genes in the 25 DAF seeds, resulting in 4,982, 19,111, and 839 differentially expressed unisequences, respectively. NIL-9 had higher seed oil content than NIL-1 under all of the temperatures in the experiments, especially at high temperatures. A total of 39 genes, among which six are located at qOC.C2.2, were differentially expressed among the NILs regardless of temperature, indicating the core genetic divergence that was unaffected by temperature. Increasing the temperature caused a reduction in seed oil content that was accompanied by the downregulation of a number of genes associated with red light response, photosynthesis, response to gibberellic acid stimulus, and translational elongation, as well as several genes of importance in the lipid metabolism pathway. These results contribute to our knowledge of the molecular nature of QTLs and the interaction between genotype and temperature. PMID:22042481

  11. Alterations in the predicted regulatory and coding regions of the sterol 14α-demethylase gene (CYP51) confer decreased azole sensitivity in the oilseed rape pathogen Pyrenopeziza brassicae.

    PubMed

    Carter, Helen E; Fraaije, Bart A; West, Jonathan S; Kelly, Steven L; Mehl, Andreas; Shaw, Michael W; Cools, Hans J

    2014-06-01

    The incidence and severity of light leaf spot epidemics caused by the ascomycete fungus Pyrenopeziza brassicae on UK oilseed rape crops are increasing. The disease is currently controlled by a combination of host resistance, cultural practices and fungicide applications. We report decreases in sensitivity of modern UK P. brassicae isolates to the azole (imidazole and triazole) class of fungicides. By cloning and sequencing the P. brassicae CYP51 (PbCYP51) gene, encoding the azole target sterol 14α-demethylase, we identified two non-synonymous mutations encoding substitutions G460S and S508T associated with reduced azole sensitivity. We confirmed the impact of the encoded PbCYP51 changes on azole sensitivity and protein activity by heterologous expression in a Saccharomyces cerevisiae mutant YUG37:erg11 carrying a controllable promoter of native CYP51 expression. In addition, we identified insertions in the predicted regulatory regions of PbCYP51 in isolates with reduced azole sensitivity. The presence of these insertions was associated with enhanced transcription of PbCYP51 in response to subinhibitory concentrations of the azole fungicide tebuconazole. Genetic analysis of in vitro crosses of sensitive and resistant isolates confirmed the impact of PbCYP51 alterations in coding and regulatory sequences on a reduced sensitivity phenotype, as well as identifying a second major gene at another locus contributing to resistance in some isolates. The least sensitive field isolates carry combinations of upstream insertions and non-synonymous mutations, suggesting that PbCYP51 evolution is ongoing and the progressive decline in azole sensitivity of UK P. brassicae populations will continue. The implications for the future control of light leaf spot are discussed. PMID:24298976

  12. What Is Rape and Date Rape?

    MedlinePlus

    ... What is rape and date rape? What is rape and date rape? Get help National Sexual Assault Hotline 800-656- ... drinking, it is NOT your fault. What is sexual assault? Sexual assault and abuse is any kind of ...

  13. Rape (sexual assault) - overview

    MedlinePlus

    Sex and rape; Date rape; Sexual assault ... Rape may occur between members of the same sex. This is more common in places such as prisons, military settings, and single-sex schools. People with physical or mental disabilities or ...

  14. Post-harvest N2O emissions were not affected by various types of oilseed straw incorporated into soil

    NASA Astrophysics Data System (ADS)

    Köbke, Sarah; Senbayram, Mehmet; Hegewald, Hannes; Christen, Olaf; Dittert, Klaus

    2015-04-01

    Oilseed rape post-harvest N2O emissions are seen highly critical as so far they are considered as one of the most crucial drawbacks in climate-saving bioenergy production systems. N2O emissions may substantially counterbalance the intended savings in CO2 emissions. Carbon-rich crop residues in conjunction with residual soil nitrate are seen as a key driver since they may serve as energy source for denitrification and, they may alter soil-borne N2O emissions. As oilseed rape straw is known to have high N/C ratio compared to other crop residues, its soil incorporation may specifically trigger post-harvest N2O emissions. Therefore, the aim of the present study was to determine post-harvest N2O emissions in soils amended with various types of oilseed rape straw (with different N/C ratio) and barley straw in field and incubation experiments. In the incubation experiment, oilseed rape or 15N labelled barley straw were mixed with soil at a rate of 1.3 t DM ha-1 and studied for 43 days. Treatments consisted of non-treated control soil (CK), 15N labelled barley straw (BST), oilseed rape straw (RST), 15N labelled barley straw + N (BST+N), or oilseed rape straw + N (RST+N). N fertilizer was applied to the soil surface as ammonium-nitrate at a rate of 100 kg N ha-1 and soil moisture was adjusted to 80% water-holding capacity. In the field experiment, during the vegetation period 15N labelled fertilizer (15NH415NO3) was used to generate 15N labelled oilseed rape straw (up to 5 at%). Here, the three fertilizer treatments consisted of 5 kg N ha-1 (RST-5), 150 kg N ha-1 (RST-150) and 180 kg N ha-1 (RST-180). Post-harvest N2O emissions were determined during the period of August 2013 to February 2014 by using static flux chambers. In the incubation trial, cumulative N2O emissions were 5, 29, 40 g N2O-N ha-1 148 days-1 in non-fertilized control, BST and RST treatments, respectively. Here, emissions were slightly higher in RST than BST (p

  15. Oilseeds for renewable jet fuel

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A multi-disciplinary research project was initiated to investigate the agronomic performance of different oilseed species under varying conditions across the western U.S. wheat belt, provide regionalized strategies to integrate sustainable oilseed production into existing land uses, and provide stra...

  16. Comparison of oilseed yields: a preliminary review

    SciTech Connect

    Duke, J.A.; Bagby, M.O.

    1982-01-01

    It was assumed that for most oilseed crops, 90% of the oil yield might be considered as profit. To compare oil seeds, pertinent portions of the yield and energy paragraphs from a summary published by Dr. Duke for DOE Grant No. 59-2246-1-6-054-0 with Dr. Bagby as ADODR were reproduced. The seed yields ranged from 200 to 14,000 kg/ha, the low one too low to consider and the high one suspiciously high. The yield of 14,000 kg oil per hectare is equivalent to more than 30 barrels of oil per hectare. The energy species included ambrette, tung-oil tree, cashew, wood-oil tree, mu-oil tree, peanut, mustard greens; rape, colza; black mustard, turnip, safflower, colocynth, coconut, crambe, African oil palm, soybean, cotton, sunflower, Eastern black walnut, Engligh walnut, meadow foam, flax, macadamia nuts, opium poppy, perilla, almond, castorbean, Chinese tallow tree, sesame, jojoba, yellow mustard, stokes' aster, and Zanzibar oilvine. 1 table. (DP)

  17. Rape Myths, Rape Scripts, and Common Rape Experiences of College Women: Differences in Perceptions of Women Who Have Been Raped.

    PubMed

    Hockett, Jericho M; Saucier, Donald A; Badke, Caitlyn

    2016-03-01

    Rape is prevalent at colleges. Although research suggests commonalities across many college women's rape experiences (e.g., perpetrators using multiple coercive strategies), vignettes used to assess rape perceptions often reflect false beliefs. Two studies varying a perpetrator's coercive tactics examine rape perceptions using vignettes reflecting rape myths, rape scripts, or many college women's common rape experiences. Participants perceive a woman who was raped more positively in vignettes reflecting common rape experiences versus those reflecting rape myths or scripts. Theoretical, educational, and research implications are discussed. PMID:26276120

  18. Adolescent Attitudes about Rape.

    ERIC Educational Resources Information Center

    Kershner, Ruth

    1996-01-01

    A very significant problem in society is adolescent rape victimization and the growing number of adolescent perpetrators. This paper examines adolescent attitudes about rape in order to develop curricular materials. It is found that adolescents exhibit conservative attitudes about gender roles, general rape myths, and victim issues. (Author)

  19. Attitudes toward Rape.

    ERIC Educational Resources Information Center

    Larsen, Knud S.; Long, Ed

    While the perception of rape has been studied intensely in recent years, most scales focus on rape myths as the content domain. The need exists for a more general instrument which would include items from a variety of sources, e.g., attitudes toward rape awareness, sexual history, age, virginity, community support, etc., in addition to the myth of…

  20. Victims of Rape.

    ERIC Educational Resources Information Center

    Krasner William; And Others

    A scientific, multidisciplinary examination of the social and psychological effects of rape upon female victims is presented, which is then correlated with: (1) the circumstances that surrounded the rape; (2) the victim's personality and social adaptation before she was raped; and (3) the support available from organizations and people who were…

  1. The Realities of Date Rape.

    ERIC Educational Resources Information Center

    Presley, Cara; Watson, Jennifer; Williams, Audrey R.

    This poster presentation addresses the issue of date rape, specifically in the college environment. Highlighted are date rape statistics, demographics, and date rape drugs. Also discussed are date rape warnings and prevention strategies. It is concluded that college and university administrators must place the issue of date rape and acquaintance…

  2. NCP oilseed processing pilot plant

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There are very few toll processors that can perform small-scale processing of oilseeds, oils and co-products in one location and none is situated in the Midwest. In the past, our pilot-scale trials were conducted in different facilities in the U.S. and Canada. To address this limitation, the NCP p...

  3. Planting depth for oilseed calendula

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Calendula (Calendula officinalis L.) is not only a popular ornamental plant in temperate climates, but also a potential oilseed crop. Its seed oil has high levels of calendic acid, which makes it a highly valued drying oil with important industrial applications. Much basic agronomic information on c...

  4. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.

    PubMed

    Chalhoub, Boulos; Denoeud, France; Liu, Shengyi; Parkin, Isobel A P; Tang, Haibao; Wang, Xiyin; Chiquet, Julien; Belcram, Harry; Tong, Chaobo; Samans, Birgit; Corréa, Margot; Da Silva, Corinne; Just, Jérémy; Falentin, Cyril; Koh, Chu Shin; Le Clainche, Isabelle; Bernard, Maria; Bento, Pascal; Noel, Benjamin; Labadie, Karine; Alberti, Adriana; Charles, Mathieu; Arnaud, Dominique; Guo, Hui; Daviaud, Christian; Alamery, Salman; Jabbari, Kamel; Zhao, Meixia; Edger, Patrick P; Chelaifa, Houda; Tack, David; Lassalle, Gilles; Mestiri, Imen; Schnel, Nicolas; Le Paslier, Marie-Christine; Fan, Guangyi; Renault, Victor; Bayer, Philippe E; Golicz, Agnieszka A; Manoli, Sahana; Lee, Tae-Ho; Thi, Vinh Ha Dinh; Chalabi, Smahane; Hu, Qiong; Fan, Chuchuan; Tollenaere, Reece; Lu, Yunhai; Battail, Christophe; Shen, Jinxiong; Sidebottom, Christine H D; Wang, Xinfa; Canaguier, Aurélie; Chauveau, Aurélie; Bérard, Aurélie; Deniot, Gwenaëlle; Guan, Mei; Liu, Zhongsong; Sun, Fengming; Lim, Yong Pyo; Lyons, Eric; Town, Christopher D; Bancroft, Ian; Wang, Xiaowu; Meng, Jinling; Ma, Jianxin; Pires, J Chris; King, Graham J; Brunel, Dominique; Delourme, Régine; Renard, Michel; Aury, Jean-Marc; Adams, Keith L; Batley, Jacqueline; Snowdon, Rod J; Tost, Jorg; Edwards, David; Zhou, Yongming; Hua, Wei; Sharpe, Andrew G; Paterson, Andrew H; Guan, Chunyun; Wincker, Patrick

    2014-08-22

    Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and B. oleracea, followed by chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms and high gene content. We examined the B. napus genome and the consequences of its recent duplication. The constituent An and Cn subgenomes are engaged in subtle structural, functional, and epigenetic cross-talk, with abundant homeologous exchanges. Incipient gene loss and expression divergence have begun. Selection in B. napus oilseed types has accelerated the loss of glucosinolate genes, while preserving expansion of oil biosynthesis genes. These processes provide insights into allopolyploid evolution and its relationship with crop domestication and improvement. PMID:25146293

  5. Are We Encouraging Rape?

    ERIC Educational Resources Information Center

    Campbell, Patricia B.

    The purpose of this paper was to increase awareness of some of the ways that we, as individuals and as a society, are encouraging the crime of rape. The paper examines our ideals about sexuality, appropriate sexual roles for women and men, the relationship between sex and aggression and how these contribute to rape. Laws, court procedures and…

  6. Rape: A Family Crisis.

    ERIC Educational Resources Information Center

    White, Priscilla N.; Rollins, Judith C.

    1981-01-01

    Rape is a crisis shared by the victim and her family. The family's reaction is influenced by cultural views such as viewing rape as sex rather than violence. Adaptive responses can be supported by open expression, education, and family, as well as individual counseling. (JAC)

  7. Naming the Rape Victim.

    ERIC Educational Resources Information Center

    Clay, Nolan

    Since state laws prohibiting identification of rape victims were struck down in a 1975 United States Supreme Court ruling, the media have been reconsidering their traditional policy of preserving victims' anonymity. Explaining their decision to begin naming victims in rape trials, several newspapers cite the press's responsibility to provide the…

  8. Experimental Study of Reciprocating Friction between Rape Stalk and Bionic Nonsmooth Surface Units.

    PubMed

    Ma, Zheng; Li, Yaoming; Xu, Lizhang

    2015-01-01

    Background. China is the largest producer of rape oilseed in the world; however, the mechanization level of rape harvest is relatively low, because rape materials easily adhere to the cleaning screens of combine harvesters, resulting in significant cleaning losses. Previous studies have shown that bionic nonsmooth surface cleaning screens restrain the adhesion of rape materials, but the underlying mechanisms remain unclear. Objective. The reciprocating friction between rape stalk and bionic nonsmooth metal surface was examined. Methods. The short-time Fourier transform method was used to discriminate the stable phase of friction signals and the stick-lag distance was defined to analyze the stable reciprocating friction in a phase diagram. Results. The reciprocating friction between rape stalk and metal surface is a typical stick-slip friction, and the bionic nonsmooth metal surfaces with concave or convex units reduced friction force with increasing reciprocating frequency. The results also showed that the stick-lag distance of convex surface increased with reciprocating frequency, which indicated that convex surface reduces friction force more efficiently. Conclusions. We suggest that bionic nonsmooth surface cleaning screens, especially with convex units, restrain the adhesion of rape materials more efficiently compared to the smooth surface cleaning screens. PMID:27034611

  9. Experimental Study of Reciprocating Friction between Rape Stalk and Bionic Nonsmooth Surface Units

    PubMed Central

    Ma, Zheng; Li, Yaoming; Xu, Lizhang

    2015-01-01

    Background. China is the largest producer of rape oilseed in the world; however, the mechanization level of rape harvest is relatively low, because rape materials easily adhere to the cleaning screens of combine harvesters, resulting in significant cleaning losses. Previous studies have shown that bionic nonsmooth surface cleaning screens restrain the adhesion of rape materials, but the underlying mechanisms remain unclear. Objective. The reciprocating friction between rape stalk and bionic nonsmooth metal surface was examined. Methods. The short-time Fourier transform method was used to discriminate the stable phase of friction signals and the stick-lag distance was defined to analyze the stable reciprocating friction in a phase diagram. Results. The reciprocating friction between rape stalk and metal surface is a typical stick-slip friction, and the bionic nonsmooth metal surfaces with concave or convex units reduced friction force with increasing reciprocating frequency. The results also showed that the stick-lag distance of convex surface increased with reciprocating frequency, which indicated that convex surface reduces friction force more efficiently. Conclusions. We suggest that bionic nonsmooth surface cleaning screens, especially with convex units, restrain the adhesion of rape materials more efficiently compared to the smooth surface cleaning screens. PMID:27034611

  10. Explaining wartime rape.

    PubMed

    Gottschall, Jonathan

    2004-05-01

    In the years since the first reports of mass rapes in the Yugoslavian wars of secession and the genocidal massacres in Rwanda, feminist activists and scholars, human rights organizations, journalists, and social scientists have dedicated unprecedented efforts to document, explain, and seek solutions for the phenomenon of wartime rape. While contributors to this literature agree on much, there is no consensus on causal factors. This paper provides a brief overview of the literature on wartime rape in historical and ethnographical societies and a critical analysis of the four leading explanations for its root causes: the feminist theory, the cultural pathology theory, the strategic rape theory, and the biosocial theory. The paper concludes that the biosocial theory is the only one capable of bringing all the phenomena associated with wartime rape into a single explanatory context. PMID:15326538

  11. Gender Differences in Rape Reporting.

    ERIC Educational Resources Information Center

    Pino, Nathan W.; Meier, Robert F.

    1999-01-01

    Compares male and female rape reporting behavior using data from the National Crime and Victimization Survey for 897 rape victims. Data indicate that the situational characteristics of rape and factors that influence reporting a rape differ by sex. Women reported victimization more frequently than did men. (SLD)

  12. Is the Rape Rate Increasing?

    ERIC Educational Resources Information Center

    Cameron, Paul; And Others

    While it has been asserted that rape has increased in frequency over the past generation, part of this increase may be due to a greater willingness to report rape to the authorities. A study was conducted to examine the frequency of rape and to describe the characteristics of rape victims. A random questionnaire survey of 4,340 adults in five…

  13. Rape (sexual assault) - overview

    MedlinePlus

    ... This is more common in places such as prisons, military settings, and single-sex schools. People with ... rape. Explain ways to reduce the odds of HIV infection if the rapist may have had HIV. ...

  14. Secondary victims of rape.

    PubMed

    Christiansen, Dorte; Bak, Rikke; Elklit, Ask

    2012-01-01

    Rape is often a very traumatic experience, which affects not only the primary victim (PV) but also his/her significant others. Studies on secondary victims of rape are few and have almost exclusively studied male partners of female rape victims. This study examined the impact of rape on 107 secondary victims, including family members, partners, and friends of male and female rape victims. We found that many respondents found it difficult to support the PV and that their relationship with the PV was often affected by the assault. Furthermore, the sample showed significant levels of traumatization, and it was estimated that approximately one quarter of the respondents suffered from posttraumatic stress syndrome (PTSD). Degree of traumatization was associated with a more recent assault, higher efforts to support the PV, recurrent thoughts about having been able to prevent the assault, a lack of social support for the respondent, and feeling let down by others. The respondents were generally interested in friend-, family-, and partner-focused interventions, particularly in receiving education about how best to support a rape victim. PMID:22594219

  15. Campus Gang Rape: Party Games?

    ERIC Educational Resources Information Center

    Ehrhart, Julie K.; Sandler, Bernice R.

    The phenomenon of gang rape as it sometimes occurs on college campuses is described, with attention to causes, impacts on the victim and other students, responses the college should take, and prevention. Consideration is given to the role of alcohol, drugs, and pornography in fraternity gang rape; successful model programs for rape prevention…

  16. Production and fuel characteristics of vegetable oil from oilseed crops in the Pacific Northwest

    SciTech Connect

    Auld, D.L.; Bettis, B.L.; Peterson, C.L.

    1982-01-01

    The purpose of this research was to evaluate the potential yield and fuel quality of various oilseed crops adapted to the Pacific Northwest as a source of liquid fuel for diesel engines. The seed yield and oil production of three cultivars of winter rape (Brassica napus L.), two cultivars of safflower (Carthamus tinctorius L.) and two cultivars of sunflower (Helianthus annuus L.) were evaluated in replicated plots at Moscow. Additional trials were conducted at several locations in Idaho, Oregon and Washington. Sunflower, oleic and linoleic safflower, and low and high erucic acid rapeseed were evaluated for fatty acid composition, energy content, viscosity and engine performance in short term tests. During 20 minute engine tests power output, fuel economy and thermal efficiency were compared to diesel fuel. Winter rape produced over twice as much farm extractable oil as either safflower or sunflower. The winter rape cultivars, Norde and Jet Neuf had oil yields which averaged 1740 and 1540 L/ha, respectively. Vegetable oils contained 94 to 95% of the KJ/L of diesel fuel, but were 11.1 to 17.6 times more viscous. Viscosity of the vegetable oils was closely related to fatty acid chain length and number of unsaturated bonds (R/sup 2/=.99). During short term engine tests all vegetable oils produced power outputs equivalent to diesel, and had thermal efficiencies 1.8 to 2.8% higher than diesel. Based on these results it appears that species and cultivars of oilseed crops to be utilized as a source of fuel should be selected on the basis of oil yield. 1 figure, 5 tables.

  17. Perennial Grain and Oilseed Crops.

    PubMed

    Kantar, Michael B; Tyl, Catrin E; Dorn, Kevin M; Zhang, Xiaofei; Jungers, Jacob M; Kaser, Joe M; Schendel, Rachel R; Eckberg, James O; Runck, Bryan C; Bunzel, Mirko; Jordan, Nick R; Stupar, Robert M; Marks, M David; Anderson, James A; Johnson, Gregg A; Sheaffer, Craig C; Schoenfuss, Tonya C; Ismail, Baraem; Heimpel, George E; Wyse, Donald L

    2016-04-29

    Historically, agroecosystems have been designed to produce food. Modern societies now demand more from food systems-not only food, fuel, and fiber, but also a variety of ecosystem services. And although today's farming practices are producing unprecedented yields, they are also contributing to ecosystem problems such as soil erosion, greenhouse gas emissions, and water pollution. This review highlights the potential benefits of perennial grains and oilseeds and discusses recent progress in their development. Because of perennials' extended growing season and deep root systems, they may require less fertilizer, help prevent runoff, and be more drought tolerant than annuals. Their production is expected to reduce tillage, which could positively affect biodiversity. End-use possibilities involve food, feed, fuel, and nonfood bioproducts. Fostering multidisciplinary collaborations will be essential for the successful integration of perennials into commercial cropping and food-processing systems. PMID:26789233

  18. Mycotoxin production on rice, pulses and oilseeds

    NASA Astrophysics Data System (ADS)

    Begum, Fouzia; Samajpati, N.

    Mycotoxin-producing fungi were isolated from contaminated grains of rice, pulses and oilseeds sold in the local markets of Calcutta for human consumption. It was found that aflatoxin B1 was produced by Aspergillus flavus and Aspergillus parasiticus, aflatoxin G1 by A. flavus, ochratoxin by Aspergillus ochraceous, sterigmatocystin by Aspergillus japonicus and citrinin by Penicillium citrinum. Aflatoxin B1 (333-10416μg/kg) was produced by Aspergillus spp. in rice, pulses and oilseeds.

  19. [Bacterial diseases of rape].

    PubMed

    Zakharova, O M; Mel'nychuk, M D; Dankevych, L A; Patyka, V P

    2012-01-01

    Bacterial destruction of the culture was described and its agents identified in the spring and winter rape crops. Typical symptoms are the following: browning of stem tissue and its mucilagization, chlorosis of leaves, yellowing and beginning of soft rot in the place of leaf stalks affixion to stems, loss of pigmentation (violet). Pathogenic properties of the collection strains and morphological, cultural, physiological, and biochemical properties of the agents of rape's bacterial diseases isolated by the authors have been investigated. It was found that all the isolates selected by the authors are highly or moderately aggressive towards different varieties of rape. According to the complex of phenotypic properties 44% of the total number of isolates selected by the authors are related to representatives of the genus Pseudomonas, 37% - to Xanthomonas and 19% - to Pectobacterium. PMID:23293826

  20. An Acquaintance Rape Prevention Program: Effects on Attitudes Toward Women, Rape-Related Attitudes, and Perceptions of Rape Scenarios.

    ERIC Educational Resources Information Center

    Pinzone-Glover, Holly A.; Gidycz, Christine A.; Jacobs, Cecilia Dine

    1998-01-01

    Studied the effects of an acquaintance-rape prevention program on college students' attitudes (n=152) toward rape and attitudes toward women, perceptions of acquaintance rape scenarios, and rape empathy. Positive attitude change was stronger among men, who became more certain of rape definitions. Contains 53 references. (SLD)

  1. Social Perception of Rape: How Rape Myth Acceptance Modulates the Influence of Situational Factors

    ERIC Educational Resources Information Center

    Frese, Bettina; Moya, Miguel; Megias, Jesus

    2004-01-01

    This study assessed the role of rape myth acceptance (RMA) and situational factors in the perception of three different rape scenarios (date rape, marital rape, and stranger rape). One hundred and eighty-two psychology undergraduates were asked to emit four judgements about each rape situation: victim responsibility, perpetrator responsibility,…

  2. Why Do Women Accept the Rape Myth?

    ERIC Educational Resources Information Center

    Tabone, Christopher; And Others

    The rape myth, defined as prejudicial, stereotyped, or false beliefs about rape, rape victims, and rapists, is accepted by individuals from varied walks of life, including women. It has been suggested that rape myth acceptance (RMA) among women serves a protective function by enabling women to dissociate themselves from a rape victim's experience.…

  3. Attitudes toward rape and victims of rape: a test of the feminist theory in Ghana.

    PubMed

    Boakye, Kofi E

    2009-10-01

    This study explores the usefulness of the feminist theory in explaining attitudes toward rape and victims of rape in Ghana. The feminist theory of rape posits, inter alia, that patriarchy and gender inequality are major factors in the aetiology of rape and attitudes toward rape and that underlying patriarchy and gender inequality are gender stereotypes and false beliefs (myths) about rape, rapists, and victims of rape. Thus, the theory suggests a relationship between rape myths and less favorable attitudes toward rape and victims of rape. Results from a survey conducted in Ghana show some support for the feminist theory of rape: There is evidence of rape myth acceptance in Ghana; gender is significant in predicting levels of rape myth acceptance; and finally, education or profession and age, but not religion, are associated with levels of rape myth acceptance in a predictable way. PMID:19252064

  4. The costs of rape.

    PubMed

    Perilloux, Carin; Duntley, Joshua D; Buss, David M

    2012-10-01

    The current study examined costs experienced by victims of completed rape (n=49) and attempted sexual assault (n=91) using quantitative analyses of 13 domains: health, self-esteem, self-perceived attractiveness, self-perceived mate value, family relationships,work life, social life, social reputation, sexual reputation, desire to have sex, frequency of sex, enjoyment of sex, and long-term, committed relationships. Women also provided descriptive accounts of their experiences, and we used these to illustrate the costs in the victims' own words.Compared to victims of an attempted sexual assault, victims of a completed rape reported significantly more negative outcomes in 11 of the 13 domains. The most negatively affected domains were self-esteem, sexual reputation, frequency of sex, desire to have sex, and self-perceived mate value. Although victims of rape experienced more negative effects than victims of attempted sexual assault,both groups of victims reported negative effects in every domain.Discussion focuses on the implications of the differing degrees and patterns of the costs of attempted and completed sexual victimization. PMID:21975924

  5. Rape Myth Consistency and Gender Differences in Perceiving Rape Victims: A Meta-Analysis.

    PubMed

    Hockett, Jericho M; Smith, Sara J; Klausing, Cathleen D; Saucier, Donald A

    2016-02-01

    An overview discusses feminist analyses of oppression, attitudes toward rape victims, and previously studied predictors of individuals' attitudes toward rape victims. To better understand such attitudes, this meta-analysis examines the moderating influences of various rape victim, perpetrator, and crime characteristics' rape myth consistency on gender differences in individuals' perceptions of rape victims (i.e., victim responsibility and blame attributions and rape minimizing attitudes). Consistent with feminist theoretical predictions, results indicated that, overall, men perceived rape victims more negatively than women did. However, this sex difference was moderated by the rape myth consistency within the rape vignettes. Implications for research are discussed. PMID:26446194

  6. Rape Myth Acceptance and Rape Proclivity: Expected Dominance Versus Expected Arousal as Mediators in Acquaintance-Rape Situations

    ERIC Educational Resources Information Center

    Chiroro, Patrick; Bohner, Gerd; Viki, G. Tendayi; Jarvis, Christopher I.

    2004-01-01

    Individuals who are high in rape myth acceptance (RMA) have been found to report a high proclivity to rape. In a series of three studies, the authors examined whether the relationship between RMA and self-reported rape proclivity was mediated by anticipated sexual arousal or anticipated enjoyment of sexually dominating the rape victim. Results of…

  7. Industrial oilseed crops and ecosystem services: Pollinators

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Many industrial and specialty oilseed crops have showy flowers that are highly attractive to insect pollinators. Moreover, across the spectrum of species and cultivars that can be grown in frigid regions, such as Minnesota, floral phenology can be manipulated to vary from early spring (April) to lat...

  8. Oilseed Productivity Under Varying Water Availability

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Oilseeds for biofuel production may serve as one of the alternative energy strategies that the United States will employ in the future. Biodiesel can be produced from oil extracted from canola, mustard, camelina, sunflower, safflower, and soybean. This paper compares soil water extraction and water ...

  9. Breeding oilseed crops for climate change

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Oilseed crops are the basis for biological systems that produce edible oils, contribute to renewable energy production, help stabilize greenhouse gases, and mitigate the risk of climate change. Their response to climate change will be dictated by reactions to temperature, carbon dioxide, solar radia...

  10. Acquaintance Rape: Effective Avoidance Strategies.

    ERIC Educational Resources Information Center

    Levine-MacCombie, Joyce; Koss, Mary P.

    1986-01-01

    Determined that acknowledged and unacknowledged acquaintance rape victims and rape avoiders could be discriminated by situational variables and response strategies. Avoiders were less likely to have experienced passive or internalizing emotions at the time of the assault, perceived the assault as less violent, and were more likely to have utilized…

  11. Gender Differences in Rape Supportive Attitudes before and after a Date Rape Education Intervention.

    ERIC Educational Resources Information Center

    Lenihan, Genie O.; And Others

    1992-01-01

    Assessed university students' attitudes toward rape and rape mythology and measured impact on these attitudes following exposure to acquaintance rape education program. Findings from 821 students revealed that women readily changed rape supportive attitudes, whereas men were resistant to date rape education program. Findings suggest need for new…

  12. Date and Acquaintance Rape: The Legal Point of View. Part 1. The Traditional Law of Rape.

    ERIC Educational Resources Information Center

    Julian, Frank H.

    1993-01-01

    First article in two-part series on date and acquaintance rape explains unique circumstances of rape trials and looks at development of traditional rape law. Examines judicial history of rape, and reviews elements of rape (penetration, intent, force, and consent). (Author/NB)

  13. Attitudes toward Rape and Victims of Rape: A Test of the Feminist Theory in Ghana

    ERIC Educational Resources Information Center

    Boakye, Kofi E.

    2009-01-01

    This study explores the usefulness of the feminist theory in explaining attitudes toward rape and victims of rape in Ghana. The feminist theory of rape posits, inter alia, that patriarchy and gender inequality are major factors in the aetiology of rape and attitudes toward rape and that underlying patriarchy and gender inequality are gender…

  14. Rape Prevention: Experiential Training for Men.

    ERIC Educational Resources Information Center

    Lee, Lucienne A.

    This report describes the Rape Prevention Education Project, an experiential program designed to educate men about the myths and facts of rape and to increase their empathic understanding of the rape victim. The four parts of the educational workshops contained in the program are listed: (1) defining rape, explaining the severity of the problem,…

  15. Reactions to Stranger and Acquaintance Rape.

    ERIC Educational Resources Information Center

    Tetreault, Patricia A.; Barnett, Mark A.

    1987-01-01

    Investigated reactions to a woman who presumably had been raped by a stranger or an acquaintance. Undergraduates read one of two rape descriptions prior to watching a videotape of the woman who (they were led to believe) had been the victim of the rape. Females and males showed markedly different reactions to stranger and acquaintance rape and…

  16. ASD and PTSD in Rape Victims

    ERIC Educational Resources Information Center

    Elklit, Ask; Christiansen, Dorte M.

    2010-01-01

    In recent years, a number of studies have investigated the prediction of posttraumatic stress disorder (PTSD) through the presence of acute stress disorder (ASD). The predictive power of ASD on PTSD was examined in a population of 148 female rape victims who visited a center for rape victims shortly after the rape or attempted rape. The PTSD…

  17. Rape: Legal issues in mental health perspective

    PubMed Central

    Jiloha, R. C.

    2013-01-01

    Rape of women by men has occurred throughout recorded history and across cultures and religions. It is a crime against basic human right and a most common crime against women in India. In this article, rape is discussed from legal and mental health perspective. In India ‘rape laws’ began with enactment of Indian Penal Code in 1860. There have been subsequent amendments and the main issue of focus remained the definition of ‘rape and inclusion of ‘marital rape’ in the ambit of rape. Law Commission Reports related to rape and the psychological impacts of rape have been discussed. PMID:24082245

  18. Reflections on researching rape resistance.

    PubMed

    Ullman, Sarah E

    2014-03-01

    This article provides a retrospective account of my experience embarking on research about women's resistance to rape, including reflections on personal and professional experiences related to studying this topic. I discuss factors inspiring my interest, including pioneering feminist rape researchers, my experience as a woman living with the reality and fear of rape, and influential mentors who facilitated my career development as a scholar in graduate school and beyond. I weave this narrative together with my thoughts about how the study of resistance relates to other important issues in the field of sexual assault including alcohol, recovery, and prevention. PMID:24626768

  19. Predicting Rape Victim Empathy Based on Rape Victimization and Acknowledgment Labeling.

    PubMed

    Osman, Suzanne L

    2016-06-01

    Two studies examined rape victim empathy based on personal rape victimization and acknowledgment labeling. Female undergraduates (Study 1, n = 267; Study 2, n = 381) from a Northeast U.S. midsize public university completed the Rape-Victim Empathy Scale and Sexual Experiences Survey. As predicted, both studies found that acknowledged "rape" victims reported greater empathy than unacknowledged victims and nonvictims. Unexpectedly, these latter two groups did not differ. Study 1 also found that acknowledged "rape" victims reported greater empathy than victims who acknowledged being "sexually victimized." Findings suggest that being raped and acknowledging "rape" together may facilitate rape victim empathy. PMID:26490506

  20. Precursors to rape: pressuring behaviors and rape proclivity.

    PubMed

    Strain, Megan L; Hockett, Jericho M; Saucier, Donald A

    2015-01-01

    We developed measures assessing personal and normative attitudes toward two types of behaviors that are symptomatic of rape culture. We conceptualize sexual violence as existing on a continuum and argue that two types of behaviors may be potential antecedents to (and consequences of) sexual violence: attempts to pressure, which mimic the power dynamics of rape in a less aggressive fashion, and benevolent dating behaviors, which are accepted dating scripts in which men initiate action. We examined individuals' acceptance of these behaviors in relation to their attitudes toward rape victims and among men to rape proclivity. This initial work suggests that these constructs and measures may be useful to investigate in future research. PMID:25929145

  1. If Your Child is Raped

    MedlinePlus

    ... for someone whose been raped, such as a forensic nurse examiner (FNE) or sexual assault nurse examiner ( ... doctor. © 1995- The Nemours Foundation. All rights reserved. Images provided by The Nemours Foundation, iStock, Getty Images, ...

  2. Management of the rape victim.

    PubMed

    Enos, W F; Beyer, J C

    1978-09-01

    If a rape crisis center and specialized personnel are not available, the family physician can adequately examine and treat the rape victim as well as collect and properly handle all the required medical evidence. The detailed history has several facets. Laboratory confirmation can be obtained for prior consensual intercourse, sodomy and other special aspects. Treatment is indicated for the prevention of venereal disease and pregnancy. Psychologic support is essential. The physician's examination and testimony can play an important legal role. PMID:685803

  3. [Extragenital injuries in rape].

    PubMed

    Kernbach, G; Püschel, K; Brinkmann, B

    1984-10-01

    The documentation of extragenital injuries to verify violent cohabitation has considerable forensic relevance. 1,875 police files in Hamburg were investigated. Injuries were analysed under the following aspects: kinds, localisations and patterns. According to the most frequent conduct of the doer (manual fixation of the victim and beats in 60% of all cases), consequences of contused violence (i.e. effusions of blood, contusions, dehiscences) were found in 50% of all cases. Injuries due to semi-sharp violence, wounds of genitals or signs of choke were relatively rare (15-3-3%). An isolated state of genital organs is of minor importance. The most frequent localisations of injuries were face and arms, less frequently legs and back. Injuries as result of manual fixation, defence or abutment formed the main patterns. "Banal" hurts were also found. In numerous cases, the outer appearance of the lesions enabled conclusions regarding the instrument used (i.e. double wales after beats with a stick). Self-inflicted injuries can mostly be discerned by their kind and localisation. Rape without any injuries is conceivable as the result of missing defence in the presence of severe threat. Psychic alterations were found in 20% of the cases, half of them continuing for a long time. The medical examination of the suspected man must include the search of signs of a fight (scratch marks, bites), localised at the hands, face and neck (besides ascertainment of traces, for example, smears of semen and smegma, i.e. from the glans penis). Extent and thoroughness of medical examinations as presently practised are discussed critically. The manner in which these examinations are conducted, is considered to be the main cause of a "secondary victimisation" of the raped woman. PMID:6569844

  4. An Assessment of College Students' Attitudes and Empathy toward Rape.

    ERIC Educational Resources Information Center

    Chng, Chwee Lye; Burke, Sloane

    1999-01-01

    College students (n=387) completed the "attitudes toward rape" questionnaire and the rape empathy scale. Analysis found that prior experience as a rape survivor and gender were significantly related to rape intolerant attitudes and empathy toward rape survivors. Rape tolerant attitudes and lower levels of empathy were more commonly found among men…

  5. Beyond Rape Myths: A More Complex View of Perceptions of Rape Victims.

    ERIC Educational Resources Information Center

    Buddie, Amy M.; Miller, Arthur G.

    2001-01-01

    Examined personal beliefs and perceptions of cultural stereotypes surrounding rape victims. Primarily white college students listed their beliefs and perceptions and rated a specific rape victim accordingly. Students' personal beliefs tended to focus more on perceptions of victim reactions to the rape rather than rape myths. However, their…

  6. A Study of Women Who Both Were Raped and Avoided Rape.

    ERIC Educational Resources Information Center

    Bart, Pauline B.

    1981-01-01

    This article, based on interviews with women who had both been raped and avoided being raped when attacked, examines the situational circumstances under which women were more likely to be raped, and those under which they were more likely to avoid being raped. (Author/MJL)

  7. Rape reporting: "Classic Rape" and the behavior of law.

    PubMed

    Clay-Warner, Jody; McMahon-Howard, Jennifer

    2009-01-01

    Two theories of rape reporting, the Classic Rape perspective and Black's Theory of the Behavior of Law, are tested in this article. We offer the first comprehensive multivariate test of Classic Rape predictions among a nationally representative sample of victims, as well as the first test of Black's predictions for rape reporting. Through the construction of multinomial regression models, we are able to examine reporting patterns for both victims and third parties. Weapon use and physical injury consistently predicted reporting. The likelihood of victim reporting significantly increased when assaults occurred either in public or through a "home blitz," whereas place of assault did not affect the likelihood of third-party reporting. On the other hand, victim-offender relationship significantly affected the likelihood of third-party reporting but was not significant in the victim-reporting models. There were mixed findings regarding Black's stratification and morphology predictions, and we found no significant effects for culture, organization, or social control. Overall, these findings lend greater support to the Classic Rape perspective than to Black's model. PMID:20055211

  8. Oilseed Rape Planting Area Extraction by Support Vector Machine Using Landsat TM Data

    NASA Astrophysics Data System (ADS)

    Wang, Yuan; Huang, Jingfeng; Wang, Xiuzhen; Wang, Fumin; Liu, Zhanyu; Xu, Junfeng

    One parametric classify (Maximum likelihood classify, MLC for short) and two non-parametric classifiers (Adaptive resonance theory mappings and Support vector machines, ARTMAP and SVM for short) were presented in this study. Base on the confusion matrix and the pixels fuzzy analysis, the nonparametric classifier may be a more preferable approach than the parametric classifier for some remote sensing applications and deserves further investigation. The ARTMAP classify represent much best than the rest of classify, especially for the grade of pure pixel (90-100% pureness), Kappa coefficients and overall accuracy were nearly 100%. The higher pureness the pixels were, the better classification accuracy was got.

  9. Sequencing-based variant detection in the polyploid crop oilseed rape

    PubMed Central

    2013-01-01

    Background The detection and exploitation of genetic variation underpins crop improvement. However, the polyploid nature of the genomes of many of our most important crops represents a barrier, particularly for the analysis of variation within genes. To overcome this, we aimed to develop methodologies based on amplicon sequencing that involve the incorporation of barcoded amplification tags (BATs) into PCR products. Results A protocol was developed to tag PCR products with 5’ 6-base oligonucleotide barcode extensions before pooling for sequencing library production using standard Illumina adapters. A computational method was developed for the de-convolution of products and the robust detection and scoring of sequence variants. Using this methodology, amplicons targeted to gene sequences were screened across a B. napus mapping population and the resulting allele scoring strings for 24 markers linkage mapped to the expected regions of the genome. Furthermore, using one-dimensional 8-fold pooling, 4608 lines of a B. napus mutation population were screened for induced mutations in a locus-specific amplicon (an orthologue of GL2.b) and mixed product of three co-amplified loci (orthologues of FAD2), identifying 10 and 41 mutants respectively. Conclusions The utilisation of barcode tags to de-convolute pooled PCR products in multiplexed, variation screening via Illumina sequencing provides a cost effective method for SNP genotyping and mutation detection and, potentially, markers for causative changes, even in polyploid species. Combining this approach with existing Illumina multiplexing workflows allows the analysis of thousands of lines cheaply and efficiently in a single sequencing run with minimal library production costs. PMID:23915099

  10. Hybridization and backcrossing between transgenic oilseed rape and two related weed species under field conditions.

    PubMed

    Halfhill, Matthew D; Zhu, Bin; Warwick, Suzanne I; Raymer, Paul L; Millwood, Reginald J; Weissinger, Arthur K; Stewart, C Neal

    2004-01-01

    Determining the frequency of crop-wild transgene flow under field conditions is a necessity for the development of regulatory strategies to manage transgenic hybrids. Gene flow of green fluorescent protein (GFP) and Bacillus thuringiensis (Bt) transgenes was quantified in three field experiments using eleven independent transformed Brassica napus L. lines and the wild relatives, B. rapa L. and Raphanus raphanistrum L. Under a high crop to wild relative ratio (600:1), hybridization frequency with B. rapa differed among the individual transformed B. napus lines (ranging from ca. 4% to 22%), however, this difference could be caused by the insertion events or other factors, e.g., differences in the hybridization frequencies among the B. rapa plants. The average hybridization frequency over all transformed lines was close to 10%. No hybridization with R. raphanistrum was detected. Under a lower crop to wild relative ratio (180:1), hybridization frequency with B. rapa was consistent among the transformed B. napus lines at ca. 2%. Interspecific hybridization was higher when B. rapa occurred within the B. napus plot (ca. 37.2%) compared with plot margins (ca. 5.2%). No significant differences were detected among marginal plants grown at 1, 2, and 3 m from the field plot. Transgene backcrossing frequency between B. rapa and transgenic hybrids was determined in two field experiments in which the wild relative to transgenic hybrid ratio was 5-15 plants of B. rapa to 1 transgenic hybrid. As expected, ca. 50% of the seeds produced were transgenic backcrosses when the transgenic hybrid plants served as the maternal parent. When B. rapa plants served as the maternal parent, transgene backcrossing frequencies were 0.088% and 0.060%. Results show that transgene flow from many independent transformed lines of B. napus to B. rapa can occur under a range of field conditions, and that transgenic hybrids have a high potential to produce transgenic seeds in backcrosses. PMID:15612504

  11. Plant defence responses in oilseed rape MINELESS plants after attack by the cabbage moth Mamestra brassicae

    PubMed Central

    Ahuja, Ishita; van Dam, Nicole Marie; Winge, Per; Trælnes, Marianne; Heydarova, Aysel; Rohloff, Jens; Langaas, Mette; Bones, Atle Magnar

    2015-01-01

    The Brassicaceae family is characterized by a unique defence mechanism known as the ‘glucosinolate–myrosinase’ system. When insect herbivores attack plant tissues, glucosinolates are hydrolysed by the enzyme myrosinase (EC 3.2.1.147) into a variety of degradation products, which can deter further herbivory. This process has been described as ‘the mustard oil bomb’. Additionally, insect damage induces the production of glucosinolates, myrosinase, and other defences. Brassica napus seeds have been genetically modified to remove myrosinase-containing myrosin cells. These plants are termed MINELESS because they lack myrosin cells, the so-called toxic mustard oil mines. Here, we examined the interaction between B. napus wild-type and MINELESS plants and the larvae of the cabbage moth Mamestra brassicae. No-choice feeding experiments showed that M. brassicae larvae gained less weight and showed stunted growth when feeding on MINELESS plants compared to feeding on wild-type plants. M. brassicae feeding didn’t affect myrosinase activity in MINELESS plants, but did reduce it in wild-type seedlings. M. brassicae feeding increased the levels of indol-3-yl-methyl, 1-methoxy-indol-3-yl-methyl, and total glucosinolates in both wild-type and MINELESS seedlings. M. brassicae feeding affected the levels of glucosinolate hydrolysis products in both wild-type and MINELESS plants. Transcriptional analysis showed that 494 and 159 genes were differentially regulated after M. brassicae feeding on wild-type and MINELESS seedlings, respectively. Taken together, the outcomes are very interesting in terms of analysing the role of myrosin cells and the glucosinolate–myrosinase defence system in response to a generalist cabbage moth, suggesting that similar studies with other generalist or specialist insect herbivores, including above- and below-ground herbivores, would be useful. PMID:25563968

  12. Plant defence responses in oilseed rape MINELESS plants after attack by the cabbage moth Mamestra brassicae.

    PubMed

    Ahuja, Ishita; van Dam, Nicole Marie; Winge, Per; Trælnes, Marianne; Heydarova, Aysel; Rohloff, Jens; Langaas, Mette; Bones, Atle Magnar

    2015-02-01

    The Brassicaceae family is characterized by a unique defence mechanism known as the 'glucosinolate-myrosinase' system. When insect herbivores attack plant tissues, glucosinolates are hydrolysed by the enzyme myrosinase (EC 3.2.1.147) into a variety of degradation products, which can deter further herbivory. This process has been described as 'the mustard oil bomb'. Additionally, insect damage induces the production of glucosinolates, myrosinase, and other defences. Brassica napus seeds have been genetically modified to remove myrosinase-containing myrosin cells. These plants are termed MINELESS because they lack myrosin cells, the so-called toxic mustard oil mines. Here, we examined the interaction between B. napus wild-type and MINELESS plants and the larvae of the cabbage moth Mamestra brassicae. No-choice feeding experiments showed that M. brassicae larvae gained less weight and showed stunted growth when feeding on MINELESS plants compared to feeding on wild-type plants. M. brassicae feeding didn't affect myrosinase activity in MINELESS plants, but did reduce it in wild-type seedlings. M. brassicae feeding increased the levels of indol-3-yl-methyl, 1-methoxy-indol-3-yl-methyl, and total glucosinolates in both wild-type and MINELESS seedlings. M. brassicae feeding affected the levels of glucosinolate hydrolysis products in both wild-type and MINELESS plants. Transcriptional analysis showed that 494 and 159 genes were differentially regulated after M. brassicae feeding on wild-type and MINELESS seedlings, respectively. Taken together, the outcomes are very interesting in terms of analysing the role of myrosin cells and the glucosinolate-myrosinase defence system in response to a generalist cabbage moth, suggesting that similar studies with other generalist or specialist insect herbivores, including above- and below-ground herbivores, would be useful. PMID:25563968

  13. Rape myth acceptance impacts the reporting of rape to the police: a study of incarcerated women.

    PubMed

    Heath, Nicole M; Lynch, Shannon M; Fritch, April M; Wong, Maria M

    2013-09-01

    We examined the association between rape myth acceptance (RMA) and reporting rapes to the police. Situational characteristics of the rape (e.g., stranger attack, injury) are known predictors of reporting, but no existing studies have examined the association between beliefs about rape and reporting. In addition, most studies of RMA do not assess victimization history. Incarcerated women experience high rates of sexual assaults prior to incarceration. We recruited 74 rape survivors from a northwestern state prison. Results suggest that women who endorsed higher levels of RMA were less likely to report their rapes to police; however, participants endorsed few rape myths. PMID:24142951

  14. Oppression through acceptance?: predicting rape myth acceptance and attitudes toward rape victims.

    PubMed

    Hockett, Jericho M; Saucier, Donald A; Hoffman, Bethany H; Smith, Sara J; Craig, Adam W

    2009-08-01

    Feminist theories of rape motivation are based on research suggesting a relationship between dominance and sexual aggression. However, the relationship between dominance and rape myth acceptance (RMA), a predictor of rape proclivity and sexual aggression and a key component in feminist theory, is understudied. The current study tests the hypotheses that individuals' scores on sex-based oppression and intergroup dominance measures will improve the predictive models for RMA and attitudes toward rape and rape victims. The hypotheses are supported. Individuals' general intergroup dominance and sex-based oppression attitudes provide significant unique prediction beyond previously studied predictors of attitudes about rape and rape victims. PMID:19506093

  15. Male rape victim and perpetrator blaming.

    PubMed

    Sleath, Emma; Bull, Ray

    2010-06-01

    One of four possible vignettes manipulated by (a) level of rape myth contained within them (low vs. high) and (b) type of rape (stranger vs. acquaintance) was presented to participants followed by scales measuring victim blame, perpetrator blame, belief in a just world, sex-role egalitarian beliefs, and male rape myth acceptance. Victim blaming was predicted by male rape myth acceptance. Perpetrator blaming was predicted by male rape myth acceptance and sex-role egalitarianism. Differences were found in victim and perpetrator blaming in terms of stranger and acquaintance rape and also in relation to manipulating the level of rape myths. Findings are discussed in relation to the previous literature on rape victim and perpetrator blaming. PMID:19738198

  16. Adolescent attitudes toward victim precipitation of rape.

    PubMed

    Marciniak, L M

    1998-01-01

    Adolescents have high rates of rape victimization and offending compared to other age groups, yet few studies have examined predictors of rape-supportive attitudes among adolescents. Drawing from Burt's (1980) study of rape myth acceptance among adults, this study tests a path analytic model of adolescent attitudes about victim precipitation of rape, using a sample of 1393 cases from the National Youth Survey (NYS) (Elliott, Ageton, Huizinga, Knowles, & Cantor, 1983). LISREL (Jöreskeg & Sörbom, 1988) estimation procedures are applied. Results show direct effects of sociocultural influences on an individual's level of rape myth acceptance, but demonstrate some interesting gender differences. Findings also reveal indirect effects of age, race, and socioeconomic status on attitudes toward rape, which operate through traditional gender role stereo-typing. Educating young adolescents about the nature of rape and the rights and roles of women are logical points of intervention to decrease acceptance of rape myths that target the victim. PMID:9836415

  17. Disconnected Lives: Men, Masculinity, and Rape Prevention.

    ERIC Educational Resources Information Center

    Capraro, Rocco L.

    1994-01-01

    Discusses rape prevention in the context of a problematic masculinity. Reviews conservative, mythopoetic, and feminist perspectives on masculinity. Proposes a feminist foundation for rape prevention programs for college males. (Author/KW)

  18. 7 CFR 1412.32 - Direct payment yield for designated oilseed and pulse crops.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... oilseed and pulse crops. (a) The direct payment yield for designated oilseeds for which a yield was not... designated oilseed for which a yield was not established by September 30, 2007, and for pulse crops on the... 7 Agriculture 10 2013-01-01 2013-01-01 false Direct payment yield for designated oilseed and...

  19. 7 CFR 1412.32 - Direct payment yield for designated oilseed and pulse crops.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... oilseed and pulse crops. (a) The direct payment yield for designated oilseeds for which a yield was not... designated oilseed for which a yield was not established by September 30, 2007, and for pulse crops on the... 7 Agriculture 10 2012-01-01 2012-01-01 false Direct payment yield for designated oilseed and...

  20. 7 CFR 1412.32 - Direct payment yield for designated oilseed and pulse crops.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... oilseed and pulse crops. (a) The direct payment yield for designated oilseeds for which a yield was not... designated oilseed for which a yield was not established by September 30, 2007, and for pulse crops on the... 7 Agriculture 10 2014-01-01 2014-01-01 false Direct payment yield for designated oilseed and...

  1. Preparation of food supplements from oilseed cakes.

    PubMed

    Sunil, L; Appaiah, Prakruthi; Prasanth Kumar, P K; Gopala Krishna, A G

    2015-05-01

    Oilseed cakes have been in use for feed preparation. Being rich in proteins, antioxidants, fibers, vitamins and minerals, oilseed cakes have been considered ideal for food supplementation. These oilseed cakes can be processed and made more palatable and edible by suitable treatments and then incorporated as food supplements for human consumption. Rice bran pellets (RBP), stabilized rice bran (SRB), coconut cake (CC) and sesame cake (SC) were taken up for the study. These were mixed with distilled water and cooked in such a way to separate the cooked solid residue and liquid extract followed by freeze drying to get two products from each. The raw, cooked dried residue and extract were analyzed for various parameters such as moisture (0.9-27.4 %), fat (2.1-16.1 %), ash (3.3-9.0 %), minerals (2.6-633.2 mg/100 g), total dietary fiber (23.2-58.2 %), crude fiber (2.7-10.5 %), protein (3.2-34.0 %), and the fat further analyzed for fatty acid composition, oryzanol (138-258 mg/100 g) and lignan (99-113 mg/100 g) contents and also evaluated sensory evaluation. Nutritional composition of products as affected by cooking was studied. The cooked products (residue and extract) showed changes in nutrients content and composition from that of the starting cakes and raw materials, but retained more nutrients in cooked residue than in the extract. The sensory evaluation of cooked residue and extract showed overall higher acceptability by the panelists than the starting cakes and raw materials. On the basis of these findings it can be concluded that these cooked residue and extract products are highly valuable for food supplementation than the raw ones. PMID:25892801

  2. Reliability Reporting Practices in Rape Myth Research.

    ERIC Educational Resources Information Center

    Buhi, Eric R.

    2005-01-01

    A number of school-based programs address sexual violence by focusing on adolescents' attitudes about rape or acceptance of rape myths. However, really problems exist in the literature regarding measurement of rape myth acceptance, including issues of reliability and validity. This paper addresses measurement reliability issues and reviews…

  3. Rape Prevention: Experiential Training for Men.

    ERIC Educational Resources Information Center

    Lee, Lucienne A.

    1987-01-01

    Describes an experiential program in which men are educated about rape and are helped to increase their empathic understanding of victims of rape. Discusses the two-hour educational workshop which focuses on rape myths and facts, guided fantasy, and experiential empathy. (Author/NB)

  4. Delayed reporting of the rape victim.

    PubMed

    Burgess, A W; Fehder, W P; Hartman, C R

    1995-09-01

    1. Delayed reporting of rape may be due to impaired cognitive processing, altered states of consciousness, or cognitive dissonance. 2. DNA may speak for the victim who does not remember a rape due to being unconscious. 3. Women with psychosis may incorporate a rape into their delusional system. PMID:7500301

  5. Male Rape Victim and Perpetrator Blaming

    ERIC Educational Resources Information Center

    Sleath, Emma; Bull, Ray

    2010-01-01

    One of four possible vignettes manipulated by (a) level of rape myth contained within them (low vs. high) and (b) type of rape (stranger vs. acquaintance) was presented to participants followed by scales measuring victim blame, perpetrator blame, belief in a just world, sex-role egalitarian beliefs, and male rape myth acceptance. Victim blaming…

  6. Cooperative involvement and opportunities in oilseeds

    SciTech Connect

    Dunn, J.R.; Reynolds, B.J.; Eversull, E.E.; Skinner, R.A.; Thurston, S.K.

    1982-01-01

    This report focuses on the opportunities for US cooperatives to improve their position in the oilseeds complex as they face increasing vertical integration and restructuring by large, competing noncooperative firms. Cooperatives operated 38 oilseed processing plants, 19 of them soybean plants, 17 cottonseed plants, 1 peanut plant, and 1 sunflower/flaxseed plant. Total cooperative soybean crushing capacity was 280 million bushels in 1979-1980, representing a 20.7% share of US crushing capacity and an 8.2% share of world crushing capacity. Cooperative soybean crushing capacity increased by 75% during the 1970's. The four largest soybean processing firms in terms of crushing capacity operated 54.5% of total US capacity. The top eight firms operated 75.1%, and the top 20 firms operated 96.4%. Eight of the top 20 soybean processing firms are cooperatives. Cooperatives operated 17 of the 78 cottonseed mills active in 1979. These mills had a total capacity of 6690 tons per day for a 35% share of total US cottonseed crushing capacity. 28 references, 11 figures, 44 tables.

  7. Social perception of rape: how rape myth acceptance modulates the influence of situational factors.

    PubMed

    Frese, Bettina; Moya, Miguel; Megías, Jesús L

    2004-02-01

    This study assessed the role of rape myth acceptance (RMA) and situational factors in the perception of three different rape scenarios (date rape, marital rape, and stranger rape). One hundred and eighty-two psychology undergraduates were asked to emit four judgements about each rape situation: victim responsibility, perpetrator responsibility, intensity of trauma, and likelihood to report the crime to the police. It was hypothesized that neither RMA nor situational factors alone can explain how rape is perceived; it is the interaction between these two factors that best account for social reactions to sexual aggression. The results generally supported the authors' hypothesis: Victim blame, estimation of trauma, and the likelihood of reporting the crime to the police were best explained by the interaction between observer characteristics, such as RMA, and situational clues. That is, the less stereotypic the rape situation was, the greater was the influence of attitudes toward rape on attributions. PMID:15005999

  8. Counseling Date Rape Survivors: Implications for College Student Personnel Professionals.

    ERIC Educational Resources Information Center

    Lynch, Sherry K.

    A general legal definition of rape is sexual intercourse forced on an individual by another against his/her will and overcoming his/her resistance. Although the female is usually referred to as the survivor and the male as the perpetrator, there are cases of male rape. Rape may be divided into stranger rape and acquaintance rape. Types of…

  9. Interpreting Rape: Differences Among Professionals and Non-Professional Resources.

    ERIC Educational Resources Information Center

    Calhoun, Lawrence G.

    Physicians and rape crisis counselors may play important roles in assisting a rape victim; their beliefs and perceptions about rape may influence their treatment of the victim. Physicians (N=10) and volunteer rape counselors (N=44) completed questionnaires focused on demographic characteristics, their experiences with rape victims, and personal…

  10. Guidelines For The Rape Examination

    PubMed Central

    Sullivan, Rebecca A.; Schaefer, Joanne; Goldstein, Frank

    1980-01-01

    The rape victim presents with legal and psychological problems as well as medical ones. In order to respond to all her needs, the physician must be aware of the legal questions to be answered in the record, the patient's psychological needs in this high stress situation, and the medical problems likely to be encountered. PMID:21293620

  11. Preferences for Rape Reduction Strategies.

    ERIC Educational Resources Information Center

    Krulewitz, Judith E.; Kahn, Arnold S.

    1983-01-01

    Surveyed 53 female and 36 male college students to examine attitudes on the perceived effectiveness of four rape reduction strategies. Generally aggressive strategies and those placing the locus of responsibility for change upon women were considered most effective. Sex role attitudes were more predictive than subject sex differences. (JAC)

  12. Calling It Rape: Differences in Experiences of Women Who Do or Do Not Label Their Sexual Assault as Rape

    ERIC Educational Resources Information Center

    Kahn, Arnold S.; Jackson, Jennifer; Kully, Christine; Badger, Kelly; Halvorsen, Jessica

    2003-01-01

    Past research had found that one-half or more of all women who have had an experience that might meet the definition of rape do not label themselves rape victims. The present study examined the actual rape experiences of 33 women who labeled their assault experience as rape and 56 women who did not label their assault experience as rape through…

  13. Biodiesel From Alternative Oilseed Feedstocks: Production and Properties

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fatty acid methyl esters were prepared and evaluated as potential biodiesel fuels from several alternative oilseed feedstocks, which included camelina (Camelina sativa L.), coriander (Coriandrum sativum L.), field mustard (Brassica juncea L.), field pennycress (Thlaspi arvense L.), and meadowfoam (L...

  14. Child rape: facets of a heinous crime.

    PubMed

    Gangrade, K D; Sooryamoorthy, R; Renjini, D

    1995-01-01

    This article discusses the extent of child rape in India, case studies of girl children in legal procedures, rape settings and perpetrators, public morality, and the nature of rape laws in India. It is concluded that there is no safe place for children. Currently, rapists are allowed to go free or are acquitted. Prevention and control of child rape must involve punishment of rapists. It is not appropriate that society ostracize the victim and her family. Victims should not remain silent. National Crime Records Bureau statistics reveal increases in rape during 1986-91. State figures are given for 1986-88. Madhya Pradesh had the highest reported incidence of rape in 1988. In 1993, Madhya Pradesh had a total of 2459 rapes. Nationally, 10,425 women were reported as raped in 1991. 51.7% were 16-30 years old. There were 1099 cases of pedophilia in 1991, which was an increase over 1990. Over 50% of the pedophilia cases were reported in Uttar Pradesh. The record of convictions shows very low figures. 1992 trial results of 276 rape cases indicated that only 46 persons were convicted. Victims suffer from psychological effects of embarrassment, disgust, depression, guilt, and even suicidal tendencies. There is police and prosecution indifference as well as social stigma and social ostracism of the victim and her family. Many cases go unreported. The case studies illustrate the difficulties for the victim of experiencing the rape and the social responses: police harassment, shame and fear, and occasionally public outrage. The case studies illustrate rape in familiar settings, such as schools, family homes, and neighbors and friends' homes; rape by policemen; and rape by political influentials. Most offenders are young, married, and socioeconomically poor. Mass media portrayals fuel the frustrations of poor and lonely men in cities. Rapists exhibit anti-social behavior or psychopathology. Sexual offenses are related to society's moral values. PMID:12158004

  15. Rape myth acceptance and rape proclivity: expected dominance versus expected arousal as mediators in acquaintance-rape situations.

    PubMed

    Chiroro, Patrick; Bohner, Gerd; Viki, G Tendayi; Jarvis, Christopher I

    2004-04-01

    Individuals who are high in rape myth acceptance (RMA) have been found to report a high proclivity to rape. In a series of three studies, the authors examined whether the relationship between RMA and self-reported rape proclivity was mediated by anticipated sexual arousal or anticipated enjoyment of sexually dominating the rape victim. Results of all three studies suggest that the anticipated enjoyment of sexual dominance mediates the relationship between RMA and rape proclivity, whereas anticipated sexual arousal does not. These findings are consistent with the feminist argument that rape and sexual violence may be motivated by men's desire to exert power over women. Theoretical and practical implications of our findings are discussed. PMID:15038883

  16. Cultural myths and supports for rape.

    PubMed

    Burt, M R

    1980-02-01

    This article describes the "rape myth" and tests hypotheses derived from social psychological and feminist theory that acceptance of rape myths can be predicted from attitudes such as sex role stereotyping, adversarial sexual beliefs, sexual conservatism, and acceptance of interpersonal violence. Personality characteristics, background characteristics, and personal exposure to rape, rape victims, and rapists are other factors used in predictions. Results from regression analysis of interview data indicate that the higher the sex role stereotyping, adversarial sexual beliefs, and acceptance of interpersonal violence, the greater a respondent's acceptance of rape myths. In addition, younger and better educated people reveal less stereotypic, adversarial, and proviolence attitudes and less rape myth acceptance. Discussion focuses on the implications of these results for understanding and changing this cultural orientation toward sexual assault. PMID:7373511

  17. Belief in Rape Myths: The Role of Gender, Attitudes Toward Women and Knowledge of Rape.

    ERIC Educational Resources Information Center

    Latta, R. Michael

    Degree of belief in rape myths as determined by participant gender, attitudes about the role of women in contemporary society, and factual knowledge of rape was investigated in a sample of 118 male and 126 female college students. The results support the general assumption that a rape ideology exists which involves components of belief in rape…

  18. Reactions to Rape Victims: Effects of Rape Circumstances, Victim's Emotional Response, and Sex of Helper.

    ERIC Educational Resources Information Center

    Krulewitz, Judith E.

    1982-01-01

    Female and male undergraduates judged impact of the rape experience on victims' psychological adjustment and indicated their likely counseling goals. Results are discussed in terms of popular assumptions about rape victims and sex differences in identification with and empathy for female rape victims. Implications for training are noted. (Author)

  19. College Women's Fears and Precautionary Behaviors Relating to Acquaintance Rape and Stranger Rape.

    ERIC Educational Resources Information Center

    Hickman, Susan E.; Muehlenhard, Charlene L.

    1997-01-01

    Studied the fears, beliefs, and behaviors of 139 college women about acquaintance and stranger rape. Women were more afraid of stranger rape and took more precautions against it but paradoxically believed that acquaintance rape was more common. Precautionary behaviors were best predicted by level of fear. Theoretical and educational implications…

  20. Victim Blaming in Rape: Effects of Victim and Perpetrator Race, Type of Rape, and Participant Racism

    ERIC Educational Resources Information Center

    George, William H.; Martinez, Lorraine J.

    2002-01-01

    Stereotypes about Black sexuality fostered hypotheses that racial factors and racism influence rape victim blaming. Predominantly White and Asian college students (170 men, 162 women) completed the Modern Racism Scale and evaluated a rape vignette varying victim race, perpetrator race, and rape type. As predicted, racial factors determined victim…

  1. Rape law: a feminist legal analysis.

    PubMed

    Benson, M

    1989-01-01

    Rape law is analyzed from the feminist perspective. Feminism can be credited with making the first serious efforts to empower women in all aspects of life, including the right to be free from sexual assault. Part 1 describes feminist theory concerning rape, Part 2 discusses some of the misogynistic images that have influenced rape law while Part 3 surveys some of the changes that have occurred in the United States, England and Israel concerning rape law. Part 4 analyzes the effects of these changes. PMID:2516214

  2. War rape, natality and genocide.

    PubMed

    Schott, Robin May

    2011-01-01

    Feminist philosophy can make an important contribution to the field of genocide studies, and issues relating to gender and war are gaining new attention. In this article I trace legal and philosophical analyses of sexual violence against women in war. I analyze the strengths and limitations of the concept of social death—introduced into this field by Claudia Card—for understanding the genocidal features of war rape, and draw on the work of Hannah Arendt to understand the central harm of genocide as an assault on natality. The threat to natality posed by the harms of rape, forced pregnancy and forced maternity lie in the potential expulsion from the public world of certain groups—including women who are victims, members of the 'enemy' group, and children born of forced birth. PMID:21941691

  3. [The Use of Specific DNA Markers for the Identification of Alleles of the FAD3 Genes in Rape (Brassica napus L.)].

    PubMed

    Lemesh, V A; Mozgova, G V; Grushetskaya, Z E; Sidorenko, E V; Pilyuk, Ya E; Bakanovskaya, A V

    2015-08-01

    A search was conducted for the alleles responsible for the quality of food-grade rapeseed oil in a collection of 21 samples of spring and winter oilseed rape of Belarusian and Russian breeding. We also developed A- and C-gene-specific DNA markers to assess the genomic polymorphisms of rape for FAD3 genes and selected plants with a low content of linolenic acid for use in the selection process. The development of a method for identifying FAD3 alleles, which control the level of linolenic acid in rapeseed oil, as well as of the design for new dCAPS markers, enabled the identification of plants homozygous for individual FAD3A and/or FAD3C genes in the F2-generation. These plants are currently involved in the selection process of new varieties with a reduced content of linolenic acid in rapeseed oil. PMID:26601489

  4. Windblown soil surface characteristics of a wheat-oilseed-fallow cropping system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The production of biofuels is dependent upon oilseed feedstocks in the Pacific Northwest United States (PNW), but evidence suggests that wind erosion may be enhanced as a result of growing oilseeds in conventional wheat rotations. Little is known concerning the impact of growing oilseeds on soil cha...

  5. 7 CFR 1412.32 - Direct payment yield for designated oilseed and pulse crops.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Direct payment yield for designated oilseed and pulse... oilseed and pulse crops. (a) The direct payment yield for designated oilseeds for which a yield was not established by September 30, 2007, and pulse crops for the farm will be determined by multiplying the...

  6. 7 CFR 1412.32 - Direct payment yield for designated oilseed and pulse crops.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false Direct payment yield for designated oilseed and pulse... Establishment of Yields for Direct and Counter-Cyclical Payments § 1412.32 Direct payment yield for designated oilseed and pulse crops. (a) The direct payment yield for designated oilseeds for which a yield was...

  7. Rape avoidance behavior among Slovak women.

    PubMed

    Prokop, Pavol

    2013-01-01

    Rape has been a recurrent adaptive problem for many species, including humans. Rape is costly to women in terms of disease transmission, partner abandonment, and unwanted pregnancy (among other costs). Therefore, behavioral strategies which allow women to avoid coercive men may have been favored by selection. In line with this evolutionary reasoning, the current research documented that physically stronger women and those in a committed romantic relationship reported more rape avoidance behavior. In addition, virgin women tended to perform more rape avoidance behavior compared with their non-virgin counterparts. Women with high conception risk perceived themselves as physically stronger, which may protect them against a potential rapist. Fear of unwanted pregnancy from rape decreased as age increased, reflecting higher fertility among younger participants. However, older women reported more rape avoidance behavior, which contradicts evolutionary predictions. The results provide some support for evolutionary hypotheses of rape avoidance behavior which suggest that woman's perception of rape is influenced by parental investment and perceived physical condition. PMID:23715617

  8. Likelihood To Rape in College Males.

    ERIC Educational Resources Information Center

    Osland, Julie A.; And Others

    1996-01-01

    Proclivities toward rape and sexual force were studied among 159 college men at a small church-affiliated college. In completing a questionnaire, 34% reported some proclivity to rape or force sex, and those who reported such proclivities held more gender stereotyped attitudes. (SLD)

  9. Japanese International Students' Attitudes toward Acquaintance Rape.

    ERIC Educational Resources Information Center

    Motoike, Janice; Stockdale, Margaret

    This study looked at the influence of an Asian sociocultural variable, loss of face or social integrity, as a predictor of perceptions of acquaintance rape among Japanese students. In addition to the expected associations between gender, sexism, and perceptions of rape, loss of face was predicted to interact with the perpetrator's reference group…

  10. How to Start a Rape Crisis Center.

    ERIC Educational Resources Information Center

    Rape Crisis Center, Washington, DC.

    This booklet, written in response to requests from throughout the nation about how a rape crisis center can be started, presents the history of the founding of the Washington, D.C. center. The booklet offers sections dealing with specific issues. A section discusses, for the rape victim, pros and cons of working with the police, together with the…

  11. From Kobe Bryant to Campus Rape

    ERIC Educational Resources Information Center

    Malveaux, Julianne

    2004-01-01

    Even though laws have been passed to protect young women--who are most at risk for rape--from sexual assault on campus, many young men think they can rape without consequence. Thus, the Campus Security Act of 1990 (now known as the Jerome Clery Act), which requires campuses to report serious crimes on campus, and the Campus Sexual Assault Victim's…

  12. Attitudes toward Acquaintance Rape and Risk Appraisal.

    ERIC Educational Resources Information Center

    Wright, Doris J.

    1999-01-01

    Examines how men and women judge the presence of risk factors in a scripted acquaintance rape scenario and if alcohol was a significant factor in assessing risk. Significant differences were found in African American and Caucasian participants' attitudes toward rape and risk appraisal. There was a negative correlation between risk appraisal and…

  13. Campus Acquaintance Rape: An Ethical Analysis.

    ERIC Educational Resources Information Center

    Haggard, William K.

    1992-01-01

    Introduces idea of including ethics in acquaintance rape prevention programming. Applies education model for ethical analysis of sexual behavior to problem of acquaintance rape among college and university students to provide practitioners with new insights which will motivate inclusion of moral and ethical objectives in future prevention…

  14. What has Reform of Rape Legislation Wrought?

    ERIC Educational Resources Information Center

    Loh, Wallace D.

    1981-01-01

    Compares the impact of common law and reform rape legislation on prosecution based on analysis of 445 forcible and statutory rape cases in King County, Washington. Concludes that the impact of the statutory reform has been mainly symbolic and educative for society at large, rather than instrumental for law enforcement. (Author/MJL)

  15. Beliefs About Rape and Women's Social Roles.

    ERIC Educational Resources Information Center

    Costin, Frank; And Others

    The hypothesis that views of rape which place women at a disadvantage are positively related to beliefs which restrict the rights and roles of women in our society is tested. Two scales, the R scale and the W scale, based on a survey of beliefs about rape (Hubert Feild) and the attitudes toward women's scale (Janet Spence and Robert Helmreich),…

  16. The Psychological Impact of Rape Victims

    ERIC Educational Resources Information Center

    Campbell, Rebecca

    2008-01-01

    This review article examines rape victims' experiences seeking postassault assistance from the legal, medical, and mental health systems and how those interactions impact their psychological well-being. This literature suggests that although some rape victims have positive, helpful experiences with social system personnel, for many victims,…

  17. Determination of trace elements in rape honey and its corresponding rape flower and stem by ICP-MS.

    PubMed

    Chen, Hui; Wang, Zhi-Bin; Chang, Qiao-Ying; Wang, Wei; Fan, Chun-Lin; Pang, Guo-Fang

    2014-02-01

    The determination of 10 trace elements including Na, Mg, P, K, Ca, Mn, Zn, Rb, Sr and Ba, in rape honey and its corresponding rape flower and stem gathered from nine sampling sites was carried out by inductively coupled plasma mass spectrometry (ICP-MS). The contents of K, P, Ca, Mg and Na were obviously higher than Zn, Rb, Mn, Sr and Ba in rape honey, rape flower and rape stem. For the first five elements, K had the highest content, followed by P, Ca, Mg and Na. However, the order of content for latter five elements was not the same in different matrixes. The contents of K, P and Ca were all higher than 1 000 mg x kg(-1) in rape flower and rape stem, while the contents of P, Ca, Mn, Zn and Rb in rape flower were slightly higher than in rape stem. It can be concluded that rape flower showed slightly higher concentrating ability for trace elements than rape stem. Based on these results, radar chart was firstly applied to research the relationship of 10 elements in rape honey and its corresponding rape flower and stem. The aim of the present work was to study the possibility of using trace elements contents in rape flower to trace the geographical and botanical origin of honey instead of rape honey. It can be found from the radar charts that the stars of rape honey, rape flower and rape stem were similar to each other. This research not only provides the basic data of trace elements in comparative study of rape honey, but also gives scientific basis for tracing the origin of rape honey according to the trace elements in corresponding rape flower that replaces those of rape honey. PMID:24822437

  18. Rape myths among Appalachian college students.

    PubMed

    Haywood, Holly; Swank, Eric

    2008-01-01

    Rape myths regularly admonish victims for supposedly provoking the violence done against them. While rape attitudes have been studied in national and urban samples, the support of rape myths in rural populations is seldom investigated. Furthermore, the few empirical studies on sexual coercion in Appalachia are mostly descriptive and rarely compare the sentiments of Appalachians and non-Appalachians. To address this gap, this study surveyed 512 college students at a public university in Eastern Kentucky. In testing an Appalachian distinctiveness question, this study revealed that Appalachian students were less likely to criticize rape victims. Students were also less inclined to condemn rape victims when they were victims themselves, came from egalitarian families, stayed in college longer, rejected modem sexism, and felt little animosity toward women. PMID:18624101

  19. Blacks' and Whites' Perceptions of Interracial and Intraracial Date Rape.

    ERIC Educational Resources Information Center

    Varelas, Nicole; Foley, Linda A.

    1998-01-01

    Analyzes influence of rape myths on victims' decisions to report rapes. Examines respondents' perceptions of responsibility and evaluation of incidents. Explains respondents with strong beliefs in rape myths contribute to a tolerance of the rapist while blaming the victim. Implies attitudes reinforce unreported rapes and a lack of fair trials.…

  20. Rape on College Campuses: Reform through Title IX.

    ERIC Educational Resources Information Center

    Steinberg, Terry Nicole

    1991-01-01

    This article first, analyzes the growing problem of campus rape; second, evaluates some college rape reduction programs; third, uses case law to demonstrate that rape should be considered sex discrimination under Title IX; and, fourth, suggests an amendment to Title IX, defining rape as sex discrimination. Appropriate implementation measures by…

  1. Social Context Variables in the Social Perception of Rape.

    ERIC Educational Resources Information Center

    Cann, Arnie; And Others

    The physical and interpersonal contexts within which rapes occur were investigated. The physical context, defined in terms of observers' perceptions of the likelihood of rapes occurring in a particular setting, produce variations in the degree of responsibility assigned to the victim. Women raped in high probability of rape settings were held as…

  2. Male Rape Myths: The Role of Gender, Violence, and Sexism

    ERIC Educational Resources Information Center

    Chapleau, Kristine M.; Oswald, Debra L.; Russell, Brenda L.

    2008-01-01

    This study investigates the structure of Struckman-Johnson and Struckman-Johnson's Male Rape Myth Scale, examines gender differences in rape myth acceptance, and explores the underlying ideologies that facilitate male rape myth acceptance. A three-factor model, with rape myths regarding Trauma, Blame, and Denial as separate subscales, is the best…

  3. Understanding Attribution of Blame in Cases of Rape: An Analysis of Participant Gender, Type of Rape and Perceived Similarity to the Victim

    ERIC Educational Resources Information Center

    Grubb, Amy Rose; Harrower, Julie

    2009-01-01

    This study examined a variety of factors that may influence attributions towards rape victims. A total of 156 participants completed a questionnaire, which included a measure of attitudes towards rape victims and a vignette depicting one of three rape scenarios (a stranger rape, date rape and seduction rape). Participants rated the extent to which…

  4. The Rape Myth and Blame-Based Beliefs of Counselors-in-Training toward Male Victims of Rape.

    ERIC Educational Resources Information Center

    Kassing, Leslee R.; Prieto, Loreto R.

    2003-01-01

    Surveyed counselors-in-training regarding their acceptance of rape myths and their willingness to make blame-based attributions toward a male victim of rape. Results suggested male counselor trainees tended to endorse the greatest degree of acceptance of rape myths. Trainees of both sexes thought that a male rape victim who showed no resistance to…

  5. Evaluations of a Date Rape Situation: Effects of Victim's Power Strategy, Rape Outcome, and Sex of Subject.

    ERIC Educational Resources Information Center

    Stevens, Laura E.; DeLamarter, William A.

    According to a recent study a rape occurs every 7 minutes and 60% of those rapes happen on dates. This study examined the impact of assault outcomes (rape, avoidance) and victim's power strategy (direct/bilateral, indirect/unilateral) on male and female evaluations of a date rape situation. College students (N=121) listened to a taped dialogue and…

  6. Hostile sexism, type of rape, and self-reported rape proclivity within a sample of Zimbabwean males.

    PubMed

    Viki, G Tendayi; Chiroro, Patrick; Abrams, Dominic

    2006-08-01

    The role of hostile sexism in accounting for rape proclivity among men was investigated using a sample of Zimbabwean students. Participants were presented with either an acquaintance rape or a stranger rape scenario and asked to respond to five questions about the scenario designed to assess rape proclivity. As expected, a significant relationship between hostile sexism and rape proclivity was obtained in the acquaintance rape but not the stranger rape condition. These results replicate previous research and suggest that hostile sexists are more likely to express their hostility toward women in situations where such behavior might be perceived as acceptable. PMID:16861333

  7. What is a typical rape? Effects of victim and participant gender in female and male rape perception.

    PubMed

    Anderson, Irina

    2007-03-01

    The study had three research aims: (1) to examine the current perception of female rape. Given recent changes in public awareness of female rape, it was predicted that respondents would conceptualize a typical female rape as an acquaintance rape rather than as the stranger rape stereotype; (2) to examine whether these perceptions differ according to respondents' gender; (3) to examine the 'cultural lag' theory of male rape, where it was hypothesized that if the public perception of male rape lags behind female rape, then a typical male rape will be conceptualized as the classic stranger rape stereotype. Findings showed that contrary to predictions, a typical female rape was conceptualized according to the stranger rape stereotype. It was also found that instead of lagging behind female rape along the stranger-acquaintance rape dimension, male rape was viewed predominantly in terms of 'other' factors (factors not found on the stranger-acquaintance dimension, e.g. victim/rapist sexual orientation, rapist calls victim names), which were erroneous, sexualizing and homophobic. PMID:17355727

  8. The effects of rape residue mulching on net global warming potential and greenhouse gas intensity from no-tillage paddy fields.

    PubMed

    Zhang, Zhi-Sheng; Cao, Cou-Gui; Guo, Li-Jin; Li, Cheng-Fang

    2014-01-01

    A field experiment was conducted to provide a complete greenhouse gas (GHG) accounting for global warming potential (GWP), net GWP, and greenhouse gas intensity (GHGI) from no-tillage (NT) paddy fields with different amounts of oilseed rape residue mulch (0, 3000, 4000, and 6000 kg dry matter (DM) ha(-1)) during a rice-growing season after 3 years of oilseed rape-rice cultivation. Residue mulching treatments showed significantly more organic carbon (C) density for the 0-20 cm soil layer at harvesting than no residue treatment. During a rice-growing season, residue mulching treatments sequestered significantly more organic C from 687 kg C ha(-1) season(-1) to 1654 kg C ha(-1) season(-1) than no residue treatment. Residue mulching significantly increased emissions of CO2 and N2O but decreased CH4 emissions. Residue mulching treatments significantly increased GWP by 9-30% but significantly decreased net GWP by 33-71% and GHGI by 35-72% relative to no residue treatment. These results suggest that agricultural economic viability and GHG mitigation can be achieved simultaneously by residue mulching on NT paddy fields in central China. PMID:25140329

  9. The Effects of Rape Residue Mulching on Net Global Warming Potential and Greenhouse Gas Intensity from No-Tillage Paddy Fields

    PubMed Central

    Zhang, Zhi-Sheng; Cao, Cou-Gui; Guo, Li-Jin; Li, Cheng-Fang

    2014-01-01

    A field experiment was conducted to provide a complete greenhouse gas (GHG) accounting for global warming potential (GWP), net GWP, and greenhouse gas intensity (GHGI) from no-tillage (NT) paddy fields with different amounts of oilseed rape residue mulch (0, 3000, 4000, and 6000 kg dry matter (DM) ha−1) during a rice-growing season after 3 years of oilseed rape-rice cultivation. Residue mulching treatments showed significantly more organic carbon (C) density for the 0–20 cm soil layer at harvesting than no residue treatment. During a rice-growing season, residue mulching treatments sequestered significantly more organic C from 687 kg C ha−1 season−1 to 1654 kg C ha−1 season−1 than no residue treatment. Residue mulching significantly increased emissions of CO2 and N2O but decreased CH4 emissions. Residue mulching treatments significantly increased GWP by 9–30% but significantly decreased net GWP by 33–71% and GHGI by 35–72% relative to no residue treatment. These results suggest that agricultural economic viability and GHG mitigation can be achieved simultaneously by residue mulching on NT paddy fields in central China. PMID:25140329

  10. Industrial oilseeds bolster "hub" crop yields when used in rotation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Lack of agroecosystem diversity across the U.S. agricultural landscape is linked to several environmental issues associated with air, water, and soil quality and biodiversity. Several new industrial oilseed crops with commercial potential, offer farmers new economic opportunities and a portfolio of ...

  11. Present and potential future oilseed production systems for biofuels

    Technology Transfer Automated Retrieval System (TEKTRAN)

    U.S. agriculture is now depended on to produce renewable energy in addition to food, feed, and fuel, which if not properly managed could threaten long-term sustainability of our agricultural lands. Biofuels produced from oilseed crops, primarily biodiesel, will be an important addition to the renewa...

  12. Reliability reporting practices in rape myth research.

    PubMed

    Buhi, Eric R

    2005-02-01

    A number of school-based programs address sexual violence by focusing on adolescents' attitudes about rape or acceptance of rape myths. However, many problems exist in the literature regarding measurement of rape myth acceptance, including issues of reliability and validity. This paper addresses measurement reliability issues and reviews reliability reporting practices of studies using the Burt Rape Myth Acceptance Scale. Less than one-half of the 68 articles examined reported reliability coefficients for the data collected. Almost one-third of the studies did not mention reliability. Examples of acceptable reliability reporting are provided. It is argued that reliability coefficients for the data actually analyzed should always be assessed and reported when interpreting program results. Implicationsfor school health research and practice are discussed. PMID:15929595

  13. Acquaintance Rape on Campus: A Literature Review.

    ERIC Educational Resources Information Center

    Benson, Dennis; And Others

    1992-01-01

    Examines issues related to acquaintance rape: cultural context, legal issues, adolescent attitudes and development, alcohol and sexual assault, the assailants, the victims, and the institutional response, and makes recommendations for a system that all colleges should have in place. (SM)

  14. College Students' Attitudes toward Date Rape and Date Rape Backlash: Implications for Prevention Programs.

    ERIC Educational Resources Information Center

    Woods, Susan; Bower, Douglas J.

    2001-01-01

    Surveyed college students regarding their acceptance of rape-myth beliefs expounded by the date rape backlash movement. Results indicated that gender, adversarial attitudes toward sexual relationships, political and sex role views, perception of false accusation vulnerability, academic honorary membership, Greek affiliation, and knowledge of a…

  15. Do Rape Education Programs Influence Rape Patterns among New York State College Students?

    ERIC Educational Resources Information Center

    Parrot, Andrea

    This study examined the differences in patterns of college student reports of rape and the availability of educational programs among college students. College students (N=994) from 26 colleges and universities in New York State were studied to determine the extent of forced sex and rape experiences. Additionally, they were studied to determine if…

  16. Attributions in Rape: Effects of Type of Rape and Victim Power Strategy.

    ERIC Educational Resources Information Center

    DeLamarter, William A.; Westervelt, Karen E.

    Recent trials have brought the issue of date rape into public awareness. It is appropriate to examine the impact of variables such as the appearance of the accuser, the relationship between the accuser and the defendant, and the behavior of the accuser and defendant on attributions of responsibility in a rape situation. This study manipulated…

  17. Rape Supportive Attitudes among Greek Students before and after a Date Rape Prevention Program.

    ERIC Educational Resources Information Center

    Lenihan, Genie O.; Rawlins, Melanie E.

    1994-01-01

    This study assessed rape supportive attitudes of sorority and fraternity members and evaluated a date rape education program with comparison to a non-Greek group studied earlier. Greek students registered more desirable scores than non-Greeks on an attitudes measure, but the education program did not improve their scores. (Author)

  18. Exploring rape myths, gendered norms, group processing, and the social context of rape among college women: a qualitative analysis.

    PubMed

    Deming, Michelle E; Covan, Eleanor Krassen; Swan, Suzanne C; Billings, Deborah L

    2013-04-01

    The purpose of this research is to explore the negotiation strategies of college women as they interpret ambiguous rape scenarios. In focus groups, 1st- and 4th-year college women were presented with a series of three vignettes depicting incidents that meet the legal criteria for rape yet are ambiguous due to the presence of cultural rape myths, contexts involving alcohol consumption, varying degrees of consent, and a known perpetrator. These contexts are critical in understanding how college women define rape. Key findings indicated many of these college women utilized rape myths and norms within their peer groups to interpret rape scenarios. PMID:23637315

  19. Prevention of post-rape psychopathology: preliminary findings of a controlled acute rape treatment study.

    PubMed

    Resnick, H; Acierno, R; Holmes, M; Kilpatrick, D G; Jager, N

    1999-01-01

    Violent sexual assault such as rape typically results in extremely high levels of acute distress. The intensity of these acute psychological reactions may play a role in later recovery, with higher levels of immediate distress associated with poorer outcome. Unfortunately, post-rape forensic evidence collection procedures may serve to increase, rather than reduce initial distress, potentially exacerbating future psychopathology. To address these concerns, an acute time-frame hospital-based video intervention was developed to: (a) minimize anxiety during forensic rape exams, and (b) prevent post-rape posttraumatic stress disorder (PTSD), panic, and anxiety. Preliminary data indicated that (1) psychological distress at the time of the exam was strongly related to PTSD symptomatology 6 weeks post-rape, and (2) the video intervention successfully reduced distress during forensic exams. PMID:10504107

  20. Heterogeneity within multiple perpetrator rapes: a national comparison of lone, duo, and 3+ perpetrator rapes.

    PubMed

    da Silva, Teresa; Woodhams, Jessica; Harkins, Leigh

    2014-12-01

    The effect of number of perpetrators involved in multiple perpetrator rapes on offense characteristics is underresearched despite beliefs that duos/dyads differ in their interactions and dynamics to groups of 3+ members. We analyzed a national sample of 336 allegations of completed and attempted rape of female victims from the United Kingdom. Rapes committed by multiple (duos and groups of 3+ perpetrators) and lone offenders were compared on offense characteristics (incorporating the approach, maintenance, and closure phases of each rape) and victim and offender sociodemographic characteristics. Significant differences between rapes committed by lone, duo, and 3+ group offenders were found for the age and ethnicity of the offenders; the type of approach used; the locations of the initial contact, assault, and release of the victims; the use of a vehicle; the precautions utilized; the verbal themes present; and the sex acts performed. These results have implications for educational prevention programs and interventions with offenders. PMID:24002932

  1. Rape, sex partnership, and substance use consequences in women veterans.

    PubMed

    Booth, Brenda M; Mengeling, Michelle; Torner, James; Sadler, Anne G

    2011-06-01

    The association of rape history and sexual partnership with alcohol and drug use consequences in women veterans is unknown. Midwestern women veterans (N = 1,004) completed a retrospective telephone interview assessing demographics, rape history, substance abuse and dependence, depression, and posttraumatic stress disorder (PTSD). One third met lifetime criteria for substance use disorder (SUD), half reported lifetime completed rape, a third childhood rape, one quarter in-military rape, 11% sex with women. Lifetime SUD was higher for women with rape history (64% vs. 44%). Women with women as sex partners had significantly higher rates of all measures of rape, and also lifetime substance use disorder. Postmilitary rape, sex partnership, and current depression were significantly associated with lifetime SUD in multivariate models (odds ratio = 2.3, 3.6, 2.1, respectively). Many women veterans have a high need for comprehensive mental health services. PMID:21567476

  2. Rape prevention with college men: evaluating risk status.

    PubMed

    Stephens, Kari A; George, William H

    2009-06-01

    This study evaluates the effectiveness of a theoretically based rape prevention intervention with college men who were at high or low risk to perpetrate sexually coercive behavior. Participants (N = 146) are randomly assigned to the intervention or control group. Outcomes include rape myth acceptance, victim empathy, attraction to sexual aggression, sex-related alcohol expectancies, and behavioral indicators, measured across three time points. Positive effects are found for rape myth acceptance, victim empathy, attraction to sexual aggression, and behavioral intentions to rape. Only rape myth acceptance and victim empathy effects sustain at the 5-week follow-up. High-risk men are generally unaffected by the intervention although low-risk men produced larger effects than the entire sample. Results suggest rape prevention studies must assess risk status moderation effects to maximize prevention for high-risk men. More research is needed to develop effective rape prevention with men who are at high risk to rape. PMID:18591366

  3. Male rape myths: the role of gender, violence, and sexism.

    PubMed

    Chapleau, Kristine M; Oswald, Debra L; Russell, Brenda L

    2008-05-01

    This study investigates the structure of Struckman-Johnson and Struckman-Johnson's Male Rape Myth Scale, examines gender differences in rape myth acceptance, and explores the underlying ideologies that facilitate male rape myth acceptance. A three-factor model, with rape myths regarding Trauma, Blame, and Denial as separate subscales, is the best fitting solution. However, the results indicate that additional scale development and validity tests are necessary. In exploratory analyses, men are more accepting of male rape myths than are women. Benevolent sexism toward men and acceptance of interpersonal violence are strong predictors of male rape myth acceptance for both men and women. Thus, the attitudes that facilitate rape myth acceptance against men appear to be similar to those that facilitate rape myth acceptance against women. Suggestions for future scale development are outlined and theoretical implications of the findings are discussed. PMID:18259049

  4. Economic feasibility of diesel fuel substitutes from oilseeds in New York State

    SciTech Connect

    Lazarus, W.F.; Pitt, R.E.

    1984-11-01

    The feasibility of producing oilseeds for feed and for a diesel fuel substitute has primarily been discussed in terms of the major oilseed producing areas. The Northeast region of the United States is a major agricultural producing area which imports large quantities of soybean meal for cattle feed. This paper considers the technical and economic feasibility of producing oilseeds for feed and fuel in New York State, which is selected as a case study for the region. The possible crops considered for expanded production are sunflowers, soybeans, and flax. It is found that if enough oilseeds are grown to replace 25% of the diesel fuel used on farms, then at most 5% of the cropland would have to be converted to oilseeds, and meal would not be produced in excess of the amount currently used. The cost of producing oil is calculated as the cost of producing the seed plus the cost of processing minus the value of the meal. Enterprise budgets are developed for estimating oilseed production costs in New York State. The cost of processing is estimated for both an industrial-size plant, which does not now exist in New York, and a small on-farm plant. It is found that the diesel fuel and vegetable oil prices would have to rise substantially before oilseeds were produced in the Northeast region for feed and fuel. Moreover, the construction of an oilseed processing facility would not necessarily stimulate production of oilseeds in the region. 22 references.

  5. Social norms and the likelihood of raping: Perceived rape myth acceptance of others affects men's rape proclivity.

    PubMed

    Bohner, Gerd; Siebler, Frank; Schmelcher, Jürgen

    2006-03-01

    Research showing that rape myth acceptance (RMA) causally affects rape proclivity (RP) was extended by examining the impact of RMA-related norms on RP. Male students (total N = 264) received feedback about the alleged responses of other students to RMA items either before (Experiment 1) or after (Experiment 2) they reported their own RMA, and then their RP was assessed using acquaintance-rape scenarios. The level of RMA feedback was varied. Results showed that higher norms led to higher RP. In Experiment 1, this effect was mediated via self-reported RMA. Experiment 2 yielded main effects of both RMA feedback and self-reported RMA and an interaction effect showing that RMA feedback was particularly influential at higher levels of own RMA. Theoretical and practical implications of these findings are discussed. PMID:16455857

  6. ASD and PTSD in rape victims.

    PubMed

    Elklit, Ask; Christiansen, Dorte M

    2010-08-01

    In recent years, a number of studies have investigated the prediction of posttraumatic stress disorder (PTSD) through the presence of acute stress disorder (ASD). The predictive power of ASD on PTSD was examined in a population of 148 female rape victims who visited a center for rape victims shortly after the rape or attempted rape. The PTSD diagnosis based solely on the three core symptom clusters was best identified by a subclinical ASD diagnosis based on all ASD criteria except dissociation. However, a full PTSD diagnosis including the A( 2) and F criteria was best identified by classifying victims according to a full ASD diagnosis. Regardless of whether cases were classified according to full PTSD status or according to meeting the criteria for the three PTSD core symptom clusters, the classification was correct only in approximately two thirds of the cases. A regression analysis based on ASD severity and sexual problems following the rape accounted for only 28% of the PTSD severity variance. In conclusion, the ASD diagnosis is not an optimal method for identifying those most at risk for PTSD. It remains to be seen whether a better way can be found. PMID:20068117

  7. Malate Synthase Activity in Cotton and Other Ungerminated Oilseeds

    PubMed Central

    Miernyk, Jan A.; Trelease, Richard N.; Choinski, John S.

    1979-01-01

    Extracts from several species and varieties of ungerminated cotton seeds plus homogenates from 18 other oilseeds (representing 11 different families) were examined for malate synthase and isocitrate lyase activity. Malate synthase activities in the various cotton seeds ranged from 35 to 129% of the units per dry seed weight found in Deltapine 16 cotton. For other oilseeds, the range was from 0.3 to 58% of Deltapine 16 cotton. Castor bean (Ricinus communis L.) had the least activity per mg dry weight (12-fold lower than the next lowest species), while Pima cotton (Gossypium barbadense L.) had the highest level (8.53 units). On a per seed basis, these values were 15 and 747 nanomoles per minute. Malate synthase activity was measurable in all seed types examined, whereas isocitrate lyase could not be detected in any of the seeds. We suggest that synthesis of malate synthase during seed development is universal among oilseeds in the absence of glyoxylate-cycle-associated isocitrate lyase activity. PMID:16660858

  8. Antimicrobial polyphenols from small tropical fruits, tea and spice oilseeds.

    PubMed

    Aman, Sahar; Naim, Asma; Siddiqi, Rahmanullah; Naz, Shahina

    2014-06-01

    The polyphenolic fractions of fruits: Terminalia catappa, Carissa carandas, Ziziphus nummularia; spice oilseeds: thymol, mustard, fenugreek and poppy seeds; and herb: green and black teas were analyzed for their total phenolics, flavonoids and antimicrobial potential. All fractions from fruits, except anthocyanin of C. carandas, displayed substantial antibacterial activity in accordance to their phenolic contents, the difference in activity being quite significant (p < 0.05), highest for T. catappa (minimum inhibitory concentration, MIC: 7.8-1000 microg/mL) and lowest for C. carandas (MIC: 62.5-1000 microg/mL). With few exceptions, both green and black teas' fractions inhibited the tested strains, however, green tea fractions (MIC: 15.63-125 microg/mL) were more active than black (MIC: 31.25-1000 microg/mL) and neutral were more active than their corresponding acidic fractions. Oil fractions of all oilseeds were found to be more active than their polyphenolic fractions, their antibacterial action decreased in the order thymol > mustard > fenugreek > poppy seeds (p < 0.05). Though the fruits used for the study are underutilized and have been emphasized for processed products, they may potentially be important to fight against pathogenic bacteria in view of their MICs. The teas and oilseeds, though a small part of total food intake, are more functional and active against the tested bacterial species and may find potential applications in therapeutics and food preservation. PMID:23703103

  9. Clinical Treatment of Nonrecent Rape: How Much Do We Know?

    ERIC Educational Resources Information Center

    Koss, Mary P.; And Others

    Although studies indicate that as many as 22 percent of women have been raped, few of these women seek help immediately after the rape. Most rape victims experience a postrape distress response which may not be resolved for some victims. Long-term symptom patterns include fear/avoidance responses, affective constriction, disturbances of…

  10. "Friends" Raping Friends. Could It Happen to You?

    ERIC Educational Resources Information Center

    Hughes, Jean O'Gorman; Sandler, Bernice R.

    This publication concerning rape committed by acquaintances and "friends" is designed to provide information and support for college students. The early warning signs and how to react to potential "acquaintance" or "date" rape are addressed. Consideration is given to why this type of rape occurs and information is provided on how to avoid date…

  11. Sex Differences in University Students' Attitudes toward Rape

    ERIC Educational Resources Information Center

    Barnett, Nona J.; Feild, Hubert S.

    1977-01-01

    This study investigated the nature of sex differences among university students' attitudes toward rape. Results showed males were quite different from females in their attitudes and tended to support many myths regarding rape. Implications of the results concerning the nature and prevention of rape on college campuses are discussed. (Author)

  12. High School and College Students' Attitudes toward Rape.

    ERIC Educational Resources Information Center

    Blumberg, Michelle L.; Lester, David

    1991-01-01

    Explored myths about rape and tendency to blame victim in high school (n=54) and college (n=46) students. High school males believed more strongly than did high school females and college males in rape myths and assigned greater blame to victims. For high school students, belief in myths about rape was associated with assigning more victim blame.…

  13. Presumed Motive in the Male and Female Perception of Rape.

    ERIC Educational Resources Information Center

    Heilbrun, Alfred B., Jr.

    1980-01-01

    Sex differences in "rape stereotypes" were investigated. Males' subjective impressions of rape emphasized the aggressive rather than the sexual motive; females did not differentiate. The stereotype of a sexually motivated rape was well organized for females. No organization was found when an aggressive motive was assumed. (Author)

  14. Reading "Rape Stories": Material Rhetoric and the Trauma of Representation.

    ERIC Educational Resources Information Center

    Hesford, Wendy S.

    1999-01-01

    Raises questions about the representability of the trauma of rape and the purposes of its representation. Focuses on how the strategic enactment of a culturally dominant rape script can potentially open up a gap within which that script can be contested and the act of rape or death resisted. Discusses pedagogical challenges of teaching the…

  15. Race and Rape: The Black Woman as Legitimate Victim.

    ERIC Educational Resources Information Center

    Williams, Linda Meyer

    Scientific investigations of the relationship between race and rape have been flawed by the acceptance of official statistics and have been influenced by prevailing myths about rape and race. This paper proposes a theoretical framework for understanding rape and race. The thesis is presented that only the black victim of sexual assault is viewed…

  16. A Men's Support Group for Significant Others of Rape Victims.

    ERIC Educational Resources Information Center

    Rodkin, Lawrence I.; And Others

    1982-01-01

    Discusses the trauma experienced by women rape victims and focuses on a men's support group for male-significant-others of women who have been raped. Describes the developmental phases through which the male significant others of rape victims achieve resolution and the impact of the male's response upon the relationship. (Author)

  17. Theoretical Consideration of Forcible Rape: A Critical Analysis.

    ERIC Educational Resources Information Center

    Clagett, Arthur F.

    1988-01-01

    Examined differences in hypothetical apperceptive fantasies of committing forcible rape, which are held by male subjects, as compared with the hypothetical apperceptive fantasies of being forcibly raped, held by the female subjects. Developed a critical analysis of social and cross-cultural variables affecting rape. (Author/ABL)

  18. Preventing Acquaintance Rape through Education. What Do We Know?

    ERIC Educational Resources Information Center

    Lonsway, Kimberly A.

    1996-01-01

    Critically reviews current rape prevention education programs, focusing on common techniques and the assumption that change in rape-supportive ideologies will decrease the actual incidence of sexual aggression. Identifies many issues that must be addressed through rigorous experimentation so that the promise of rape prevention can be realized…

  19. Acquaintance Rape on Campus: A Model for Institutional Response.

    ERIC Educational Resources Information Center

    Burling, Philip

    This booklet explains the actions that college or university administrators should take to prevent or respond to incidents of acquaintance rape ("date rape") on campus. It outlines specific preventive measures, risk management procedures, and internal disciplinary procedures for acquaintance rape cases. Institutions should define acquaintance rape…

  20. Date and Acquaintance Rape among a Sample of College Students.

    ERIC Educational Resources Information Center

    Mills, Crystal S.; Granoff, Barbara J.

    1992-01-01

    Conducted date and acquaintance rape needs-assessment survey on 106 male and 113 female college students. Thirty-two women acknowledged that they were victims of rape or attempted rape, and majority reported multiple victimizations. Eighteen men admitted to committing acts that met legal definition of sexual assault in Hawaii. (Author/NB)

  1. Acquaintance Rape: What Is the Law? Teaching Strategy.

    ERIC Educational Resources Information Center

    Larson, Pat; Ryan, Roxann

    1995-01-01

    Discusses rape issues and contends that education about it should be provided at all grade levels. Presents a lesson in which students distinguish between facts and myths of rape and identify situations in which the legal definition of rape is met. (CFR)

  2. Exposure to Pornography and Acceptance of Rape Myths.

    ERIC Educational Resources Information Center

    Allen, Mike; And Others

    1995-01-01

    Summarizes the literature examining the association between acceptance of rape myths and exposure to pornography. States that nonexperimental methodology shows that exposure to pornography does not increase rape myth acceptance, while experimental studies show that exposure to pornography increases rape myth acceptance. Concludes that experimental…

  3. 66 FR 22234 - Grants for Rape Prevention and Education

    Federal Register 2010, 2011, 2012, 2013, 2014

    2001-05-03

    ... HUMAN SERVICES Centers for Disease Control and Prevention Grants for Rape Prevention and Education.... The Rape Prevention and Education Grant Program strengthens education and training to combat violence.... CDC will award targeted grants to State Health Departments to be used for rape prevention...

  4. Working Together To Change the Rape and Violence Culture.

    ERIC Educational Resources Information Center

    Abrams, Julie M.; And Others

    This publication is a collection of 20 supporting documents for a conference program, "Working Together To Change the Rape and Violence Culture." Contents include: (1) "Presenter Contact Information"; (2) "Characteristics of Rape-Prone versus Rape-Free Cultures"; (3) "Dater's Bill of Rights"; (4) "Quotes for Discussion"; (5) "University of…

  5. Freeing Our Lives: A Feminist Analysis of Rape Prevention.

    ERIC Educational Resources Information Center

    Graff, Sunny; And Others

    The factors which contribute to women's vulnerability to rape are a lack of information about and understanding of rape; women's subordinate relationship to men; socially reinforced physical weakness and passivity; isolation from other women; isolation in the community. Prevention means more than just reducing the incidence of rape or providing…

  6. Reporting Rape in a National Sample of College Women

    ERIC Educational Resources Information Center

    Wolitzky-Taylor, Kate B.; Resnick, Heidi S.; Amstadter, Ananda B.; McCauley, Jenna L.; Ruggiero, Kenneth J.; Kilpatrick, Dean G.

    2011-01-01

    Background: Studies indicate that a small percentage of rapes are reported to law enforcement officials. Research also suggests that rapes perpetrated by a stranger are more likely to be reported and that rapes involving drugs and/or alcohol are less likely to be reported. College women represent a unique and understudied population with regard to…

  7. 3 CFR - Implementing the Prison Rape Elimination Act

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 3 The President 1 2013-01-01 2013-01-01 false Implementing the Prison Rape Elimination Act Presidential Documents Other Presidential Documents Memorandum of May 17, 2012 Implementing the Prison Rape... victim, is an assault on human dignity and an affront to American values. The Prison Rape Elimination...

  8. An Updated Measure for Assessing Subtle Rape Myths

    ERIC Educational Resources Information Center

    McMahon, Sarah; Farmer, G. Lawrence

    2011-01-01

    Social workers responsible for developing rape prevention programs on college campuses must have valid evaluation instruments. This article presents the challenges encountered by the authors when they attempted to keep rape myth measures relevant to student populations by updating the language to reflect the subtleties involved with rape myths.…

  9. Long-Term Effects of Labeling a Rape Experience

    ERIC Educational Resources Information Center

    McMullin, Darcy; White, Jacquelyn W.

    2006-01-01

    Research has found that approximately half of women who report an experience that meets the legal definition of rape do not label it rape. It has been assumed that labeling the experience as rape is necessary and beneficial for recovery; however, conflicting findings have been reported. In the present study, a longitudinal design was utilized to…

  10. Rape Myth Acceptance, Sexual Trauma History, and Posttraumatic Stress Disorder

    ERIC Educational Resources Information Center

    Baugher, Shannon N.; Elhai, Jon D.; Monroe, James R.; Gray, Matt J.

    2010-01-01

    The prediction of false rape-related beliefs (rape myth acceptance [RMA]) was examined using the Illinois Rape Myth Acceptance Scale (Payne, Lonsway, & Fitzgerald, 1999) among a nonclinical sample of 258 male and female college students. Predictor variables included measures of attitudes toward women, gender role identity (GRI), sexual trauma…

  11. The transfer of 'Polima' cytoplasmic male sterility from oilseed rape (Brassica napus) to broccoli (B. oleracea) by protoplast fusion.

    PubMed

    Yarrow, S A; Burnett, L A; Wildeman, R P; Kemble, R J

    1990-08-01

    Protoplast fusion was utilised to transfer Polima type cytoplasmic male sterility (CMS) from Brassica napus, canola cv. Polima Karat (Pol-Karat) to B. oleracea, broccoli, var. "Green Comet". Southern and RFLP analysis confirmed that four cybrids possessed nuclear genomes of broccoli with Polima mitochondria and chloroplasts. A fifth cybrid was a nuclear hybrid between broccoli and Pol-Karat, with Polima mitochondria and chloroplasts of broccoli. The broccoli type cybrids were morphologically similar to "Green Comet", while the hybrid type was an intermediate of the two fusion parents. Flowers on the cybrids were distinctive in that although they possessed a morphology typical of Polima, they had very reduced petals. The broccoli type cybrids exhibited some female fertility, albeit low, establishing potential for F1 hybrid production. PMID:24226699

  12. Removing the mustard oil bomb from seeds: transgenic ablation of myrosin cells in oilseed rape (Brassica napus) produces MINELESS seeds

    PubMed Central

    Borgen, Birgit Hafeld; Thangstad, Ole Petter; Ahuja, Ishita; Rossiter, John Trevor; Bones, Atle Magnar

    2010-01-01

    Many plant phytochemicals constitute binary enzyme–glucoside systems and function in plant defence. In brassicas, the enzyme myrosinase is confined to specific myrosin cells that separate the enzyme from its substrate; the glucosinolates. The myrosinase-catalysed release of toxic and bioactive compounds such as isothiocyanates, upon activation or tissue damage, has been termed ‘the mustard oil bomb’ and characterized as a ‘toxic mine’ in plant defence. The removal of myrosin cells and the enzyme that triggers the release of phytochemicals have been investigated by genetically modifying Brassica napus plants to remove myrosinase-storing idioblasts. A construct with the seed myrosin cell-specific Myr1.Bn1 promoter was used to express a ribonuclease, barnase. Transgenic plants ectopically expressing barnase were embryo lethal. Co-expressing barnase under the control of the Myr1.Bn1 promoter with the barnase inhibitor, barstar, under the control of the cauliflower mosaic virus 35S promoter enabled a selective and controlled death of myrosin cells without affecting plant viability. Ablation of myrosin cells was confirmed with light and electron microscopy, with immunohistological analysis and immunogold-electron microscopy analysis showing empty holes where myrosin cells normally are localized. Further evidence for a successful myrosin cell ablation comes from immunoblots showing absence of myrosinase and negligible myrosinase activity, and autolysis experiments showing negligible production of glucosinolate hydrolysis products. The plants where the myrosin defence cells have been ablated and named ‘MINELESS plants’. The epithiospecifier protein profile and glucosinolate levels were changed in MINELESS plants, pointing to localization of myrosinases and a 35 kDa epithiospecifier protein in myrosin cells and a reduced turnover of glucosinolates in MINELESS plants. PMID:20219777

  13. Genetic and Epigenetic Changes in Oilseed Rape (Brassica napus L.) Extracted from Intergeneric Allopolyploid and Additions with Orychophragmus.

    PubMed

    Gautam, Mayank; Dang, Yanwei; Ge, Xianhong; Shao, Yujiao; Li, Zaiyun

    2016-01-01

    Allopolyploidization with the merger of the genomes from different species has been shown to be associated with genetic and epigenetic changes. But the maintenance of such alterations related to one parental species after the genome is extracted from the allopolyploid remains to be detected. In this study, the genome of Brassica napus L. (2n = 38, genomes AACC) was extracted from its intergeneric allohexaploid (2n = 62, genomes AACCOO) with another crucifer Orychophragmus violaceus (2n = 24, genome OO), by backcrossing and development of alien addition lines. B. napus-type plants identified in the self-pollinated progenies of nine monosomic additions were analyzed by the methods of amplified fragment length polymorphism, sequence-specific amplified polymorphism, and methylation-sensitive amplified polymorphism. They showed modifications to certain extents in genomic components (loss and gain of DNA segments and transposons, introgression of alien DNA segments) and DNA methylation, compared with B. napus donor. The significant differences in the changes between the B. napus types extracted from these additions likely resulted from the different effects of individual alien chromosomes. Particularly, the additions which harbored the O. violaceus chromosome carrying dominant rRNA genes over those of B. napus tended to result in the development of plants which showed fewer changes, suggesting a role of the expression levels of alien rRNA genes in genomic stability. These results provided new cues for the genetic alterations in one parental genome that are maintained even after the genome becomes independent. PMID:27148282

  14. Comparative Analysis of FLC Homologues in Brassicaceae Provides Insight into Their Role in the Evolution of Oilseed Rape

    PubMed Central

    Zou, Xiaoxiao; Suppanz, Ida; Raman, Harsh; Hou, Jinna; Wang, Jing; Long, Yan; Jung, Christian; Meng, Jinling

    2012-01-01

    We identified nine FLOWERING LOCUS C homologues (BnFLC) in Brassica napus and found that the coding sequences of all BnFLCs were relatively conserved but the intronic and promoter regions were more divergent. The BnFLC homologues were mapped to six of 19 chromosomes. All of the BnFLC homologues were located in the collinear region of FLC in the Arabidopsis genome except BnFLC.A3b and BnFLC.C3b, which were mapped to noncollinear regions of chromosome A3 and C3, respectively. Four of the homologues were associated significantly with quantitative trait loci for flowering time in two mapping populations. The BnFLC homologues showed distinct expression patterns in vegetative and reproductive organs, and at different developmental stages. BnFLC.A3b was differentially expressed between the winter-type and semi-winter-type cultivars. Microsynteny analysis indicated that BnFLC.A3b might have been translocated to the present segment in a cluster with other flowering-time regulators, such as a homologue of FRIGIDA in Arabidopsis. This cluster of flowering-time genes might have conferred a selective advantage to Brassica species in terms of increased adaptability to diverse environments during their evolution and domestication process. PMID:23029223

  15. Assessing Quantitative Resistance against Leptosphaeria maculans (Phoma Stem Canker) in Brassica napus (Oilseed Rape) in Young Plants

    PubMed Central

    Huang, Yong-Ju; Qi, Aiming; King, Graham J.; Fitt, Bruce D. L.

    2014-01-01

    Quantitative resistance against Leptosphaeria maculans in Brassica napus is difficult to assess in young plants due to the long period of symptomless growth of the pathogen from the appearance of leaf lesions to the appearance of canker symptoms on the stem. By using doubled haploid (DH) lines A30 (susceptible) and C119 (with quantitative resistance), quantitative resistance against L. maculans was assessed in young plants in controlled environments at two stages: stage 1, growth of the pathogen along leaf veins/petioles towards the stem by leaf lamina inoculation; stage 2, growth in stem tissues to produce stem canker symptoms by leaf petiole inoculation. Two types of inoculum (ascospores; conidia) and three assessment methods (extent of visible necrosis; symptomless pathogen growth visualised using the GFP reporter gene; amount of pathogen DNA quantified by PCR) were used. In stage 1 assessments, significant differences were observed between lines A30 and C119 in area of leaf lesions, distance grown along veins/petioles assessed by visible necrosis or by viewing GFP and amount of L. maculans DNA in leaf petioles. In stage 2 assessments, significant differences were observed between lines A30 and C119 in severity of stem canker and amount of L. maculans DNA in stem tissues. GFP-labelled L. maculans spread more quickly from the stem cortex to the stem pith in A30 than in C119. Stem canker symptoms were produced more rapidly by using ascospore inoculum than by using conidial inoculum. These results suggest that quantitative resistance against L. maculans in B. napus can be assessed in young plants in controlled conditions. Development of methods to phenotype quantitative resistance against plant pathogens in young plants in controlled environments will help identification of stable quantitative resistance for control of crop diseases. PMID:24454767

  16. Genetic and Epigenetic Changes in Oilseed Rape (Brassica napus L.) Extracted from Intergeneric Allopolyploid and Additions with Orychophragmus

    PubMed Central

    Gautam, Mayank; Dang, Yanwei; Ge, Xianhong; Shao, Yujiao; Li, Zaiyun

    2016-01-01

    Allopolyploidization with the merger of the genomes from different species has been shown to be associated with genetic and epigenetic changes. But the maintenance of such alterations related to one parental species after the genome is extracted from the allopolyploid remains to be detected. In this study, the genome of Brassica napus L. (2n = 38, genomes AACC) was extracted from its intergeneric allohexaploid (2n = 62, genomes AACCOO) with another crucifer Orychophragmus violaceus (2n = 24, genome OO), by backcrossing and development of alien addition lines. B. napus-type plants identified in the self-pollinated progenies of nine monosomic additions were analyzed by the methods of amplified fragment length polymorphism, sequence-specific amplified polymorphism, and methylation-sensitive amplified polymorphism. They showed modifications to certain extents in genomic components (loss and gain of DNA segments and transposons, introgression of alien DNA segments) and DNA methylation, compared with B. napus donor. The significant differences in the changes between the B. napus types extracted from these additions likely resulted from the different effects of individual alien chromosomes. Particularly, the additions which harbored the O. violaceus chromosome carrying dominant rRNA genes over those of B. napus tended to result in the development of plants which showed fewer changes, suggesting a role of the expression levels of alien rRNA genes in genomic stability. These results provided new cues for the genetic alterations in one parental genome that are maintained even after the genome becomes independent. PMID:27148282

  17. Date and Acquaintance Rape: The Legal Point of View. Part 2. Reform of Rape Laws and Alternatives to Criminal Prosecution.

    ERIC Educational Resources Information Center

    Julian, Frank H.

    1993-01-01

    Second article in two-part series on date and acquaintance rape examines modern reforms in rape laws and alternatives to criminal prosecution. Explains the Rape Shield Law, designed to prevent victim's sexual history from being used against her to prove promiscuity, tendency to lie, or low moral character. Other common statutory revisions are…

  18. "Nobody Told Me It Was Rape": A Parent's Guide for Talking with Teenagers about Acquaintance Rape and Sexual Exploitation.

    ERIC Educational Resources Information Center

    Adams, Caren; Fay, Jennifer

    This book was written to help parents talk to their adolescent children about acquaintance rape and sexual exploitation. It may also be useful to family life educators presenting units on rape and sexual exploitation. Acquaintance rape and sexual exploitation are explained in the first section. The second section discusses talking with teenagers…

  19. A Community Response to Rape. Polk County Rape/Sexual Assault Care Center, Des Moines, Iowa. An Exemplary Project.

    ERIC Educational Resources Information Center

    Bryant, Gerald; Cirel, Paul

    In Polk County, Iowa, criminal justice agencies and concerned citizens work together to lessen the burden on the rape victim and to increase the chance of ultimate conviction and sentencing in rape and sexual assault cases. The Rape/Sexual Assault Care Center offers medical and social supportive services to victims, aids law enforcement and…

  20. The Effectiveness of Two Types of Rape Prevention Programs in Changing the Rape-Supportive Attitudes of College Students.

    ERIC Educational Resources Information Center

    Anderson, Linda A.; Stoelb, Matthew P.; Duggan, Peter; Hieger, Brad; Kling, Kathleen H.; Payne, June P.

    1998-01-01

    The effectiveness of two rape-prevention programs in changing college students' rape-supportive attitudes was investigated. (N=215) Conditions included an interactive mock talk show and a structured video intervention. Both interventions were effective, but attitudes were found to rebound over time. Implications for future rape-prevention…

  1. Perceptions of, and Assistance Provided to, a Hypothetical Rape Victim: Differences between Rape Disclosure Recipients and Nonrecipients

    ERIC Educational Resources Information Center

    Paul, Lisa A.; Kehn, Andre; Gray, Matt J.; Salapska-Gelleri, Joanna

    2014-01-01

    Objective: Undergraduate rape disclosure recipients' and nonrecipients' sociodemographic and life experience variables, attitudes towards rape, and responses to a hypothetical rape disclosure were compared to determine differences between them. Participants: One hundred ninety-two undergraduates at 3 universities participated in this…

  2. Rape, War, and the Socialization of Masculinity: Why Our Refusal to Give up War Ensures that Rape Cannot Be Eradicated

    ERIC Educational Resources Information Center

    Zurbriggen, Eileen L.

    2010-01-01

    Rape is endemic during war, suggesting that there may be important conceptual links between the two. A theoretical model is presented positing that rape and war are correlated because traditional (hegemonic) masculinity underlies, and is a cause of, both. An analysis of the literatures on masculinity, rape perpetration, and military socialization…

  3. Examining the Relationship between Male Rape Myth Acceptance, Female Rape Myth Acceptance, Victim Blame, Homophobia, Gender Roles, and Ambivalent Sexism

    ERIC Educational Resources Information Center

    Davies, Michelle; Gilston, Jennifer; Rogers, Paul

    2012-01-01

    The relationship between male rape myth acceptance, female rape myth acceptance, attitudes toward gay men, a series of gender role and sexism measures, victim blame and assault severity were investigated. It was predicted that men would display more negative, stereotypical attitudes than women and that male rape myth endorsement would be related…

  4. Rape stereotypes and labeling: awareness of victimization and trauma.

    PubMed

    Sarmiento, Ilaria

    2011-02-01

    For years, studies in the U.S. college population have described the phenomena of date and acquaintance rape. These phenomena have perpetuated myths regarding rape leading to the faulty labeling of the experience by the survivors themselves. Compared with the U.S., in Europe there are very few studies concerning this issue. This preliminary study in Italy comprised surveys of 210 participants who were asked to express their opinions on 2 scenarios of sexual assault (a forced rape and an acquaintance rape). Only 48% of the participants thought that acquaintance rape should be tried in a criminal court. PMID:21526600

  5. 7 CFR 1412.34 - Submitting production evidence for establishing direct payment yields for oilseeds and pulse crops.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... oilseeds and pulse crops. (a)(1) Reports of production evidence must be submitted when the owner elects to... designated oilseeds for which a yield was not established by September 30, 2007, and for pulse crops must be... direct payment yields for oilseeds and pulse crops. 1412.34 Section 1412.34 Agriculture Regulations...

  6. 7 CFR 1412.34 - Submitting production evidence for establishing direct payment yields for oilseeds and pulse crops.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... oilseeds and pulse crops. (a)(1) Reports of production evidence must be submitted when the owner elects to... designated oilseeds for which a yield was not established by September 30, 2007, and for pulse crops must be... direct payment yields for oilseeds and pulse crops. 1412.34 Section 1412.34 Agriculture Regulations...

  7. 7 CFR 1412.34 - Submitting production evidence for establishing direct payment yields for oilseeds and pulse crops.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... oilseeds and pulse crops. (a)(1) Reports of production evidence must be submitted when the owner elects to... designated oilseeds for which a yield was not established by September 30, 2007, and for pulse crops must be... direct payment yields for oilseeds and pulse crops. 1412.34 Section 1412.34 Agriculture Regulations...

  8. Are rape memories different? A comparison of rape, other unpleasant, and pleasant memories among employed women.

    PubMed

    Tromp, S; Koss, M P; Figueredo, A J; Tharan, M

    1995-10-01

    The study examined empirically-measured memory characteristics, compared pleasant and unpleasant intense memories as well as rape and other unpleasant memories, and determined whether rape memories exhibited significantly more "flashbulb" characteristics. Data consisted of responses to a mailed survey of women employees of a medical center (N = 1,037) and a university (N = 2,142). Pleasant and unpleasant memories were differentiated by feelings, consequences, and level of unexpectedness. The most powerful discriminator of rape from other unpleasant memories was the degree to which they were less clear and vivid, contained a less meaningful order, were less well-remembered, and were less thought and talked about. Few "flashbulb" characteristics discriminated among memory types. Implications for clinical work with rape survivors were discussed. PMID:8564275

  9. Blacks' and whites' perceptions of interracial and intraracial date rape.

    PubMed

    Varelas, N; Foley, L A

    1998-06-01

    The prevalence of rape myths contributes to victims' reluctance to report rapes. Black (n = 30) and White (n = 96) U.S. college students responded to the Rape Myth Scale (Burt, 1980) and read a scenario of an acquaintance rape; the race of the perpetrator and victim (Black or White) were varied. The respondents assessed the victim's and perpetrator's responsibility and evaluated the incident. As hypothesized, the respondents with strong beliefs in rape myths were more tolerant of the rapist and less tolerant of the victim than were those with weaker beliefs. There was limited support for the myth of the Black rapist and White victim; however, the myth of the Black rapist appeared particularly strong among the Black respondents. The women responded more negatively to the rapist and more positively to the victim than the men did. Such biases in attitudes toward rape could keep women from reporting rapes and accused rapists from receiving fair trials. PMID:9577729

  10. Windblown soil surface characteristics altered by oilseeds in a wheat-fallow cropping system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Oilseeds are integral to the production of biofuels and diversifying rainfed cropping systems in the Pacific Northwest United States (PNW). However, there is evidence to suggest greater potential for wind erosion when growing oilseeds in wheat rotations. Little is known concerning the impact of grow...

  11. Nitrogen use in durum and selected Brassicaceae oilseeds in two-year rotations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Brassicaceae oilseeds can serve as potential feedstocks for renewable biofuels to offset demand for petroleum-based alternatives. However, little is known about oilseed crop yield potential and N use in semiarid, wheat (Triticum spp.)-based cropping systems that dominate the northern Great Plains (N...

  12. 40 CFR 63.2855 - How do I determine the quantity of oilseed processed?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... basis refers to the oilseed chemical and physical characteristics as initially received by the source.... (b) Use Equation 1 of this section to determine the quantity of each oilseed type processed at your affected source during normal operating periods recorded within a calendar month. Equation 1 of...

  13. Ongoing development of dryland oilseed production systems in the northwestern region of the United States

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This report addresses the development of dryland oilseed crops to provide feedstock for production of biofuels in semiarid portions of the northwestern United States. Bioenergy feedstocks derived from Brassica oilseed crops have been considered for production of hydrotreated renewable jet fuel, but...

  14. Windblown dust potential from oilseed cropping systems in the Pacific Northwest United States

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The volatility of petroleum reserves and prices coupled with concerns over greenhouse gas emissions and climate change has created a worldwide interest in renewable fuels. Although advances are being made in growing oilseeds for advanced biofuels, little is known about the impact of growing oilseed ...

  15. A normalized difference yellowness index for modeling yield of Brassica oilseeds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Conspicuous yellow flowers that are present in a Brassica oilseed crop such as canola require careful consideration when selecting a spectral index for yield estimation. This study evaluated spectral indices for multispectral sensors that correlate with the seed yield of Brassica oilseed crops. A ...

  16. 7 CFR 93.15 - Fees for analytical testing of oilseeds.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... office as listed in 7 CFR 93.14(a). ... 7 Agriculture 3 2010-01-01 2010-01-01 false Fees for analytical testing of oilseeds. 93.15 Section....15 Fees for analytical testing of oilseeds. The fee charged for any laboratory analysis for...

  17. 7 CFR 1412.35 - Incorrect or false production evidence of oilseeds and pulse crops.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... false production evidence of oilseeds and pulse crops. (a) If production evidence submitted in... following: (i) Make a further yield reduction for part or all of the designated oilseeds or pulse crops on... and pulse crops. 1412.35 Section 1412.35 Agriculture Regulations of the Department of...

  18. 7 CFR 1412.35 - Incorrect or false production evidence of oilseeds and pulse crops.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... false production evidence of oilseeds and pulse crops. (a) If production evidence submitted in... following: (i) Make a further yield reduction for part or all of the designated oilseeds or pulse crops on... and pulse crops. 1412.35 Section 1412.35 Agriculture Regulations of the Department of...

  19. 7 CFR 1412.35 - Incorrect or false production evidence of oilseeds and pulse crops.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... false production evidence of oilseeds and pulse crops. (a) If production evidence submitted in... following: (i) Make a further yield reduction for part or all of the designated oilseeds or pulse crops on... and pulse crops. 1412.35 Section 1412.35 Agriculture Regulations of the Department of...

  20. Examining the relationship between male rape myth acceptance, female rape myth acceptance, victim blame, homophobia, gender roles, and ambivalent sexism.

    PubMed

    Davies, Michelle; Gilston, Jennifer; Rogers, Paul

    2012-09-01

    The relationship between male rape myth acceptance, female rape myth acceptance, attitudes toward gay men, a series of gender role and sexism measures, victim blame and assault severity were investigated. It was predicted that men would display more negative, stereotypical attitudes than women and that male rape myth endorsement would be related to, and predicted by, the other attitude and attribution scales. Respondents comprised 323 undergraduates (146 males and 177 females) from a large University in the Northwest of England. Results broadly conformed to predictions, with men generally more negative than women, and male rape myth acceptance significantly related to female rape myth acceptance, negative attitudes about gay men, gender role attitudes, and victim blame. Furthermore, male rape myth acceptance was predicted by female rape myth acceptance, gender attitudes, and victim blame. Methodological issues and implications for future work and those working with victims are discussed. PMID:22550150

  1. Stranger and Acquaintance Rape: Are There Differences in the Victim's Experience?

    ERIC Educational Resources Information Center

    Koss, Mary P.; And Others

    1988-01-01

    Examined rape by strangers and by acquaintances, including nonromantic acquaintances, casual dates, steady dates, and family members in 489 rape victims. Acquaintance rape typically involved single offender and multiple episodes, was less often perceived as rape or revealed to anyone, and, except for rapes by family members, was rated less…

  2. Suggested Acquaintance/Date Rape Education & Prevention Strategies for School Health Instruction.

    ERIC Educational Resources Information Center

    Weiler, Robert M.; Walls, Nicole A.

    Data suggest that acquaintance and date rape may account for 50-70 percent of all reported rapes in the United States. Recent findings also indicate that one in four college women have been raped or a victim of attempted rape. As most rape victims are between 15 and 24 years of age, high school-based education programs must be provided if society…

  3. Stop Blaming the Victim: A Meta-Analysis on Rape Myths

    ERIC Educational Resources Information Center

    Suarez, Eliana; Gadalla, Tahany M.

    2010-01-01

    Although male rape is being reported more often than before, the majority of rape victims continue to be women. Rape myths--false beliefs used mainly to shift the blame of rape from perpetrators to victims--are also prevalent in today's society and in many ways contribute toward the pervasiveness of rape. Despite this, there has been limited…

  4. Proposed Recruit Training Topic: Rape Awareness.

    ERIC Educational Resources Information Center

    Thompson, Janet R.

    This guide, prepared by the United States Navy Recruit Training Command, was designed to address the differential training needs of male and female recruits in the area of personal security, i.e., rape awareness/prevention. In section I, the role of the Recruit Training Command is noted, and information on the incidence of sexual assault is…

  5. Forcible Rape: An Analysis of Legal Issues.

    ERIC Educational Resources Information Center

    National Inst. of Law Enforcement and Criminal Justice (Dept. of Justice/LEAA), Washington, DC.

    Traditionally, rape has been defined as "carnal knowledge of a woman by force and against her will." The test of force has been crucial, and consent deduced. Victims were assumed to be consenting parties unless criminal circumstances could be proved. Victims were oftened questioned about past sexual behavior. Current reform efforts have attempted…

  6. Rape Within the Hispanic Family Unit.

    ERIC Educational Resources Information Center

    Quinones-Sierra, Sylvia

    Because problems such as rape are often viewed as personal concerns of "la familia" there is great tendency on the part of Hispanics to accept this crime as something that must be resolved without intervention from the police, the hospitals or the courts. Seldom will much needed therapy and auxillary type services be sought due to the extreme…

  7. Stop Rape Crisis Center: An Exemplary Project.

    ERIC Educational Resources Information Center

    Whitcomb, Debra; And Others

    An exemplary project, the Stop Rape Crisis Center in Baton Rouge, Louisiana, which was initially funded by the Law Enforcement Assistance Administration (LEAA), is described. Issues addressed include the following: (1) initlal start-up and continuing program assessment; (2) staffing and the use of volunteers; (3) coordination with law enforcement…

  8. An Evaluation of a Rape Prevention Program.

    ERIC Educational Resources Information Center

    Frazier, Patricia; And Others

    This study assessed the effectiveness of an interactive, co-educational date rape prevention program. Participants for the study (n=192, 97% Caucasian) consisted of sorority and fraternity members of a larger midwestern university. Eighty-four individuals were assigned to a control group with the rest making up the intervention group. All subjects…

  9. Coping with the Experience of Rape

    ERIC Educational Resources Information Center

    Littleton, Heather; Breitkopf, Carmen Radecki

    2006-01-01

    The coping strategies that a victim of a rape engages in can have a strong impact on the development and persistence of psychological symptoms. Research provides evidence that victims who rely heavily on avoidance strategies, such as suppression, are less likely to recover successfully than those who rely less heavily on these strategies. The…

  10. Acquaintance Rape: A Case Study Approach.

    ERIC Educational Resources Information Center

    Marinelli, Rosalie D.

    1994-01-01

    Describes a case study approach to acquaintance rape used in a Personal Health and Lifestyles course at the University of Nevada in Reno that allows students to discuss the characters in a scenario, an instructional process seen as less threatening than describing personal experiences. (MDM)

  11. Acquaintance Rape on College and University Campuses

    ERIC Educational Resources Information Center

    Romeo, Felicia F.

    2004-01-01

    While a university experience offers young women many rewards, unfortunately, there are also significant dangers, and one of these dangers is men who are sexual predators. Acquaintance rape is a sexual assault perpetrated by someone who is known to the victim. It is a violent and serious crime that is far too common an occurrence at our…

  12. Risk Appraisal in Scripted Acquaintance Rape Scenarios.

    ERIC Educational Resources Information Center

    Wright, Doris J.

    Cognitive appraisals are believed to influence how women judge or appraise risk in acquaintance interactions which lead to sexual assault. Ways in which men and women judge the presence of risk factors in scripted acquaintance rape scenarios, and whether alcohol was a significant factor in assessing risk, are examined in this paper. Participants…

  13. Numbing after rape, and depth of therapy.

    PubMed

    Barglow, Peter

    2014-01-01

    The author considers the reactions of four women who had been sexually assaulted, with a focus on the rape trauma of two women with the diagnosis of "Complex-PTSD." Both patients also had prolonged episodes of illegal drug dependence. The article investigates a variety of therapeutic responses to ameliorate disabling post-rape psychological symptoms, especially an intense feeling of numbing. Psychodynamic treatment was chosen for investigation rather than Prolonged Exposure (PET), or Cognitive Behavioral Therapy (CBT). Choice of these two treatments is supported by substantial statistical evidence. But many therapists continue to use psychoanalytic based approaches to treat rape victims. Schottenbauer et al, (2008) concluded that PET and CBT approaches had high non-response and dropout rates. Also psychodynamic comprehension may be particularly suitable for "complex PTSD" as defined below in this article. Two vignettes contrast the treatment processes and outcomes of these two women to two other patients who had been sexually assaulted, but whose psychopathology was less severe. The author proposes that full comprehension of severe numbing is essential in the selection of the best intervention strategy because this symptom (or affect) may determine the prognosis of raped patients. PMID:24818460

  14. Date-rape drugs scene in Poland.

    PubMed

    Adamowicz, Piotr; Kała, Maria

    2005-01-01

    Since the beginnings of twenty-first century in Poland increasing number of reports about the drug-facilitated sexual assaults have been observed. Many drugs have been identified as so-called "date-rape drugs", because of their pharmacological properties, especially inducing amnesia. These drugs are used for the purpose of "drugging" unsuspected victims and than raping them. In a typical scenario, the perpetrator surreptitiously adds "date-rape drug" to the alcoholic or non-alcoholic beverage of an unsuspecting person, who is subsequently sexually assaulted while under the influence of this substance. Many victims do not report the incident until several days after the event or even do not report it at all. They report the incident so late after the events because they often have problems with remember the course of incident. It causes that victim is not reliable witness for justice. Detection of "date-rape drugs" in biological fluids is unequivocal evidence of perpetration. Analysis of biological fluids collected from victims of rapes for presence of drugs was rare in Poland up to now. The aim of this study is to show the use of "date-rape drugs" in Poland. Materials for this study were from the routine casework elaborated at the Institute of Forensic Research in Kraków. APCI-LC-MS methods were applied for screening of biological fluids (blood and/or urine) for amphetamine and its 6 analogues, for 12 substances from benzodiazepine group and for quantification of the detected drugs. HPLC-DAD was used as a screening method for wide range of medicinal drugs, and NCI-GC-MS methods for determination of ketamine and tetrahydrocannabinols (delta9-tetrahydrocannabinol, 9THC) and its metabolite (11-nor-carboxy-delta9-tetrahydrocannabinol, THCCOOH). In 2000-2004, the biological fluids taken from 33 persons, both sexually assaulted or perpetrators were analysed. In 2000 and 2002 not any case of this type was registered, in 2001 only two cases were recorded. After 2003

  15. Meta-Analysis of the Prevalence of Unacknowledged Rape.

    PubMed

    Wilson, Laura C; Miller, Katherine E

    2016-04-01

    Many sexual violence survivors do not label their experiences as rape but instead use more benign labels, such as "bad sex" or "miscommunication." A meta-analysis was conducted to estimate the mean prevalence of unacknowledged rape and to inform our understanding of methodological factors that influence the detection of this phenomenon. Studies were identified using PsycINFO, PubMED, and PILOTS and were required to report the percentage of unacknowledged rape that had occurred since the age of 14 among female survivors. Moderator variables included mean participant age, recruitment source, rape definition, and unacknowledged rape definition. Twenty-eight studies (30 independent samples) containing 5,917 female rape survivors met the inclusion criteria. Based on a random effects model, the overall weighted mean percentage of unacknowledged rape was 60.4% (95% confidence interval [55.0%, 65.6%]). There was a large amount of heterogeneity, Q(29) = 445.11, p < .001, and inconsistency (I(2) = 93.5%) among included studies. The prevalence was significantly higher among college student participants compared to noncollege participants. The findings supported that over half of all female rape survivors do not acknowledge that they have been raped. The results suggest that screening tools should use behaviorally descriptive items about sexual contact, rather than using terms such as "rape." PMID:25784571

  16. An Antibody to the Castor Bean Glyoxysomal Lipase (62 kD) also Binds to a 62 kD Protein in Extracts from Many Young Oilseed Plants.

    PubMed

    Hills, M J; Beevers, H

    1987-12-01

    An antibody raised against purified glyoxysomal lipase (triacylglycerol hydrolase EC 3.1.1.3.) from castor bean (relative molecular weight of 62,000) also binds to a protein with a relative molecular weight of 62,000 in extracts of food reserve tissues from many young oilseed plants. These plants include Brassica napus L., Zea mays L., Arachis hypogaea L., Glycine max L., Gossipium hirsutum L., Cucurbita pepo L., Helianthus annuus L., Pisum sativum L., and Cicer arietinum L. The antibody caused inhibition of triacylglycerol hydrolysis by the lipases in extracts from seedlings of corn, oilseed rape, castor bean, soybean, and peanut. The pattern of antilipase binding to the 62 kilodalton protein in subcellular fractions from these other seedlings was consistent with the patterns of lipase activity reported in the literature and it is suggested that lipases from these oil seeds all have a subunit with a molecular weight of 62,000. The protein was only found in the food reserve tissues and was not present in extracts of roots and leaves of mature plants. In addition, the immunoreactive 62 kilodalton polypeptide was not detectable in lima beans and only at very low levels in kidney beans. Both these seeds are known to contain very little storage lipid and would not be expected to contain lipase. With the exception of the acid lipase of castor bean, ungerminated seeds do not generally contain active lipases. The immunoreactive 62 kilodalton protein could not be detected in the ungerminated seeds of most plants and only at very low low levels in others. PMID:16665808

  17. Oilseed crops as renewable sources of industrial chemicals

    SciTech Connect

    McKeon, T.A.; Lin, Jiann-Tsyh; Goodrich-Tanrikulu, M.

    1995-12-01

    The presence of specific functional groups on a fatty acid confers value for industrial uses. The plant kingdom contains numerous examples of plants that produce seed oils containing fatty acids with epoxy groups, hydroxyl groups, triple bonds or with unusual double bond positions. These fatty acids can be used directly or are readily modified for use in specialty lubricants, plastics and coatings. Many of these plants are not cultivated in the U.S. due to unsuitable climate or growth habit. Such plants provide a source of genes coding for enzymes that will carry out the desired fatty acid modification. Genetic technology allows the transfer of these genes into domestically grown crops such as rapeseed or soybean, with consequent production of the desired fatty acid in the seed oil. One biotechnology company has commercialized a transgenic oilseed crop with an altered fatty acid composition. This talk will review current and projected plans for developing oilseed crops to serve as renewable resources that meet current industrial needs or provide chemical feedstocks for new uses.

  18. Is Reporting of Rape on the Rise? A Comparison of Women with Reported versus Unreported Rape Experiences in the National Women's Study-Replication

    ERIC Educational Resources Information Center

    Wolitzky-Taylor, Kate B.; Resnick, Heidi S.; McCauley, Jenna L.; Amstadter, Ananda B.; Kilpatrick, Dean G.; Ruggiero, Kenneth J.

    2011-01-01

    Rape affects one in seven women nationwide. Historically, most rape victims do not report rape to law enforcement. Research is needed to identify barriers to reporting and correlates of reporting to guide policy recommendations that address such barriers. We investigated the prevalence of reporting rape among a national sample of women (N = 3,001)…

  19. Communicating/Muting Date Rape: A Co-Cultural Theoretical Analysis of Communication Factors Related to Rape Culture on a College Campus

    ERIC Educational Resources Information Center

    Burnett, Ann; Mattern, Jody L.; Herakova, Liliana L.; Kahl, David H., Jr.; Tobola, Cloy; Bornsen, Susan E.

    2009-01-01

    Previous studies suggest that college campuses foster a rape culture in which date rape (most commonly, rape of women) is an accepted part of campus activity (Buchwald, Fletcher, & Roth, 1993; Sanday, 2007). In focus groups at a Midwestern university, researchers asked students about rape as they experienced it or knew about it on campus. The…

  20. Effects of Two Versions of an Empathy-Based Rape Prevention Program on Fraternity Men's Survivor Empathy, Attitudes, and Behavioral Intent to Commit Rape or Sexual Assault

    ERIC Educational Resources Information Center

    Foubert, John D.; Newberry, Johnathan T.

    2006-01-01

    Fraternity men (N = 261) at a small to midsized public university saw one of two versions of a rape prevention program or were in a control group. Program participants reported significant increases in empathy toward rape survivors and significant declines in rape myth acceptance, likelihood of raping, and likelihood of committing sexual assault.…

  1. The incidence and experience of rape among chemically dependent women.

    PubMed

    Teets, J M

    1997-01-01

    This descriptive study investigated the incidence and experience of rape among chemically dependent women. Sixty women in a residential treatment facility were interviewed about sexual trauma they had experienced in their lives. In this sample 73% had been raped, and 45% had been raped more than once. The stories of rape were classified in five categories: rape while in the context of using, when too high to resist, while prostituting, by a significant other, and by a family member. Some 35% of the rapist described were friends of the women with whom they were using drugs. Only 20% of the rapes were reported to the police. Clinical implications for treatment of addicted women who are also survivors of violence is discussed. PMID:9460026

  2. Repeat rape and multiple offending among undetected rapists.

    PubMed

    Lisak, David; Miller, Paul M

    2002-02-01

    Pooling data from four samples in which 1,882 men were assessed for acts of interpersonal violence, we report on 120 men whose self-reported acts met legal definitions of rape or attempted rape, but who were never prosecuted by criminal justice authorities. A majority of these undetected rapists were repeat rapists, and a majority also committed other acts of interpersonal violence. The repeat rapists averaged 5.8 rapes each. The 120 rapists were responsible for 1,225 separate acts of interpersonal violence, including rape, battery, and child physical and sexual abuse. These findings mirror those from studies of incarcerated sex offenders (Abel, Becker, Mittelman, Cunningham-Rathner, Rouleau, & Murphy, 1987; Weinrott and Saylor, 1991), indicating high rates of both repeat rape and multiple types of offending. Implications for the investigation and prosecution of this so-called "hidden" rape are discussed. PMID:11991158

  3. Deceiving others/deceiving oneself: four cases of factitious rape.

    PubMed

    Feldman, M D; Ford, C V; Stone, T

    1994-07-01

    Although patients with factitious disorders typically seek the "patient" role through illness portrayals, some instead portray themselves falsely as "victims." We discuss the cases of four women who claimed to have been victims of rape; the allegations ultimately were disproved. Factitious rape may be prompted by a search for nurturance; by dissociation, leading individuals to believe that trauma earlier in life is ongoing; by a need to be rescued from real, current abuse; and by projection of anger onto specific male targets. Although dramatic, factitious rape is rare, we advocate thorough investigation of rape claims even when patients have known histories of deceptive behavior. PMID:8023207

  4. Asking what before we ask why: taxonomy, etiology, and rape.

    PubMed

    Baker, Katharine K

    2003-06-01

    This article presents a spectrum of sexual coersion. By looking at the social meaning of the different acts of coercion along the spectrum, the author suggests that most acts of sexual coercion can be classified as either rape (a sexual act with intent to do harm to the victim) or sex (a sexual act engaged in without any intent to harm the victim). Ironically, though, the author suggests that the most and least egregious acts of sexual aggression, that is, the acts we most readily identify as rape and the acts we are most reluctant to label rape are the ones that most easily evade explanation as either rape or sex. PMID:12839906

  5. Introducing the Date and Acquaintance Rape Avoidance Scale.

    PubMed

    Resendez, Josephine R; Hughes, Jamie S

    2016-01-01

    We present the Date and Acquaintance Rape Avoidance Scale (DARAS). The DARAS is a measure of a woman's behaviors used to avoid date and acquaintance rape. Three factor structures were possible. The DARAS may have measured several factors related to alcohol and drug use, self-defense, and date behaviors; 2 factors related to behaviors to avoid acquaintance versus date rape; or a single factor that represented general vigilance. The data revealed a highly reliable, 63 item single factor that was correlated with stranger rape avoidance, rejection of rape myths, hostile sexist beliefs about men, and benevolent sexist beliefs about women. The creation of the DARAS adds to the growing body of research on rape avoidance. The DARAS is key to understanding the behaviors women employ to avoid date rape. Rather than placing the responsibility for rape on the victim, the DARAS was developed as a theoretical and applied tool that can be used to improve theory and construct rape education and prevention programs. PMID:27302901

  6. Vestibular response in denatured rape oil intoxication.

    PubMed

    Gavilán, C; Gavilán, J; Abril, A

    1985-10-01

    During the spring of 1981 a massive intoxication broke out in Spain, which is presently attributed to the ingestion of denatured rape oil, and which has been called 'Toxic Syndrome' (TS). We studied 51 patients affected by the TS one year after the onset of their disease, analyzing their vestibular responses in the caloric test with the help of an analog-digital computer. A significant decrease in the number of nystagmic beats of the affected patients can be observed, while the rest of the parameters studied show similar values to those found simultaneously in 30 normal subjects. It is suggested that these differences are yet another manifestation of the polyneuropathy found in intoxication by denatured rape oil. PMID:3877134

  7. Drug-facilitated sexual assault ('date rape').

    PubMed

    Schwartz, R H; Milteer, R; LeBeau, M A

    2000-06-01

    In the past few years, drug-facilitated sexual assaults have received widespread media coverage. In addition to alcohol, the most frequently used date-rape drug, flunitrazepam (Rohypnol), a fast-acting benzodiazepine, and gamma-hydroxybutyrate (GHB) and its congeners are among the most popular drugs used for this purpose. The latter drug is easily procured at some gymnasiums, popular bars, discos, and rave clubs, as well as over the Internet. Perpetrators choose these drugs because they act rapidly, produce disinhibition and relaxation of voluntary muscles, and cause the victim to have lasting anterograde amnesia for events that occur under the influence of the drug. Alcoholic beverages potentiate the drug effects. We review several date-rape drugs, provide information on laboratory testing for them, and offer guidelines for preventing drug-facilitated sexual assault. PMID:10881768

  8. The role of the new "date rape drugs" in attributions about date rape.

    PubMed

    Girard, April L; Senn, Charlene Y

    2008-01-01

    This study investigates the effect of voluntary and involuntary drug use on attributions about sexual assault. The sample was composed of 280 randomly selected male and female undergraduate students. The type of drug used (GHB, alcohol, or none) and the voluntariness of the administration were varied in an unambiguous date rape scenario. Participants viewed sexual assault facilitated by alcohol or drugs similarly to sexual assault without drug or alcohol involvement, assigning the highest levels of responsibility and blame to the perpetrator and the lowest levels of both to the victim in these situations. In contrast, women's voluntary consumption of drugs prior to a sexual assault reduced perpetrator responsibility and blame and increased blame to the victim compared to other situations (except in some cases, voluntary drunkenness). These findings extend the limited research on date rape drugs and previous work on the influence of alcohol on date rape attributions. PMID:18087029

  9. Effect of oilseed cakes on alpha-amylase production by Bacillus licheniformis CUMC305.

    PubMed

    Krishnan, T; Chandra, A K

    1982-08-01

    The effects of oilseed cakes on extracellular thermostable alpha-amylase production by Bacillus licheniformis CUMC305 was investigated. Each oilseed cake was made of groundnut, mustard, sesame, linseed, coconut copra, madhuca, or cotton. alpha-Amylase production was considerably improved in all instances and varied with the oilseed cake concentration in basal medium containing peptone and beef extract. Maximum increases were effected by a low concentration (0.5 to 1.0%) of groundnut or coconut, a high concentration (3%) of linseed or mustard, and an Rintermediate concentration (2%) of cotton, madhuca, or sesame. The oilseed cakes made of groundnut or mustard could completely replace the conventional peptone-beef extract medium as the fermentation base for the production of alpha-amylase by B. licheniformis. The addition of corn steep liquor to cotton, linseed, sesame, or madhuca cake in the medium improved alpha-amylase production. PMID:6181738

  10. Effect of oilseed cakes on alpha-amylase production by Bacillus licheniformis CUMC305.

    PubMed Central

    Krishnan, T; Chandra, A K

    1982-01-01

    The effects of oilseed cakes on extracellular thermostable alpha-amylase production by Bacillus licheniformis CUMC305 was investigated. Each oilseed cake was made of groundnut, mustard, sesame, linseed, coconut copra, madhuca, or cotton. alpha-Amylase production was considerably improved in all instances and varied with the oilseed cake concentration in basal medium containing peptone and beef extract. Maximum increases were effected by a low concentration (0.5 to 1.0%) of groundnut or coconut, a high concentration (3%) of linseed or mustard, and an Rintermediate concentration (2%) of cotton, madhuca, or sesame. The oilseed cakes made of groundnut or mustard could completely replace the conventional peptone-beef extract medium as the fermentation base for the production of alpha-amylase by B. licheniformis. The addition of corn steep liquor to cotton, linseed, sesame, or madhuca cake in the medium improved alpha-amylase production. PMID:6181738

  11. World oilseed situation and US export opportunities, November 1983. Foreign agriculture circular

    SciTech Connect

    Not Available

    1983-11-01

    Tight supplies have continued to dominate the oilseeds and products situation since the October report, with estimated world oilseed production (including flaxseed) dropping by half a million tons. Increased projections of world soybean and peanut supplies were more than offset by reduced estimates of cottonseed output, as well as smaller reductions in prospective sunflower and rapeseed output. Forecast protein meal consumption dropped slightly from last month's level; forecast vegetable oil consumption also dropped slightly from last month's level.

  12. Antioxidant Activities and Oxidative Stabilities of Some Unconventional Oilseeds.

    PubMed

    Uluata, Sibel; Ozdemir, Nurhayat

    2012-04-01

    The oils of some unconventional oilseeds (hemp, radish, terebinth, stinging nettle, laurel) were obtained by a cold-press method in which the total oil content, fatty acids, tocopherol isomers, some metal contents (Ca, Mg, Fe, Cu), antioxidant activity and oxidative stability were determined. The total oil content was determined ranging between 30.68 and 43.12%, and the oil samples had large amounts of unsaturated fatty acids, with oleic acid and linoleic acid. Of all the oils, terebinth seed oil had the highest α-tocopherol content (102.21 ± 1.01 mg/kg oil). Laurel oilseed had the highest antiradical activity in both the DPPH and ABTS assays. The peroxide value of the non-oxidized oils ranged between 0.51 and 3.73 mequiv O(2)/kg oil. The TBARS value of the non-oxidized oils ranged between 0.68 ± 0.02 and 6.43 ± 0.48 mmol MA equiv/g oil. At 110 °C, the Rancimat induction period of the oils ranged between 1.32 and 43.44 h. The infrared spectra of the samples were recorded by FTIR spectroscopy. The absorbance values of the spectrum bands were observed and it was determined that some of the chemical groups of oxidized oils caused changes in absorbance. As a result of the present research, the analyzed oils could be evaluated as an alternative to traditionally consumed vegetable oils or as additives to them. PMID:22467958

  13. In vitro Fermentation, Digestion Kinetics and Methane Production of Oilseed Press Cakes from Biodiesel Production.

    PubMed

    Olivares-Palma, S M; Meale, S J; Pereira, L G R; Machado, F S; Carneiro, H; Lopes, F C F; Maurício, R M; Chaves, A V

    2013-08-01

    Following the extraction of oil for biodiesel production, oilseed press cakes are high in fat. As the dietary supplementation of fat is currently considered the most promising strategy of consistently depressing methanogenesis, it follows that oilseed press cakes may have a similar potential for CH4 abatement. As such, this study aimed to characterise the nutritive value of several oilseed press cakes, glycerine and soybean meal (SBM) and to examine their effects on in vitro ruminal fermentation, digestion kinetics and CH4 production. Moringa press oil seeds exhibited the greatest in sacco effective degradability (ED) of DM and CP (p<0.05). In vitro gas production (ml/g digested DM) was not affected (p = 0.70) by supplement at 48 h of incubation. In vitro DMD was increased with the supplementation of glycerine and SBM at all levels of inclusion. Moringa oilseed press cakes produced the lowest CH4 (mg/g digested DM) at 6 and 12 h of incubation (p<0.05). The findings suggest that moringa oilseed press cake at 400 g/kg DM has the greatest potential of the oilseed press cakes examined in this study, to reduce CH4 production, without adversely affecting nutrient degradability. PMID:25049890

  14. In vitro Fermentation, Digestion Kinetics and Methane Production of Oilseed Press Cakes from Biodiesel Production

    PubMed Central

    Olivares-Palma, S. M.; Meale, S. J.; Pereira, L. G. R.; Machado, F. S.; Carneiro, H.; Lopes, F. C. F.; Maurício, R. M.; Chaves, A. V.

    2013-01-01

    Following the extraction of oil for biodiesel production, oilseed press cakes are high in fat. As the dietary supplementation of fat is currently considered the most promising strategy of consistently depressing methanogenesis, it follows that oilseed press cakes may have a similar potential for CH4 abatement. As such, this study aimed to characterise the nutritive value of several oilseed press cakes, glycerine and soybean meal (SBM) and to examine their effects on in vitro ruminal fermentation, digestion kinetics and CH4 production. Moringa press oil seeds exhibited the greatest in sacco effective degradability (ED) of DM and CP (p<0.05). In vitro gas production (ml/g digested DM) was not affected (p = 0.70) by supplement at 48 h of incubation. In vitro DMD was increased with the supplementation of glycerine and SBM at all levels of inclusion. Moringa oilseed press cakes produced the lowest CH4 (mg/g digested DM) at 6 and 12 h of incubation (p<0.05). The findings suggest that moringa oilseed press cake at 400 g/kg DM has the greatest potential of the oilseed press cakes examined in this study, to reduce CH4 production, without adversely affecting nutrient degradability. PMID:25049890

  15. Hidden, Unacknowledged, Acquaintance, and Date Rape: Looking Back, Looking Forward

    ERIC Educational Resources Information Center

    Koss, Mary P.

    2011-01-01

    In this commentary, the author reflects on two articles that have been among the most highly cited publications in "Psychology of Women Quarterly" ("PWQ") over its first 35 years, "The Hidden Rape Victim: Personality, Attitudinal, and Situational Characteristics" (Koss, 1985) and "Stranger and Acquaintance Rape: Are There Differences in the…

  16. How Organizations Should Respond to Rape in the Workplace.

    ERIC Educational Resources Information Center

    Lee, Ellen; Kleiner, Brian H.

    2003-01-01

    Each year approximately 51,000 incidents of rape and sexual assault occur in the workplace. If an incident of rape occurs in the workplace, management can adopt several procedures for both the assaulted victim and the organization. This article highlights those procedures, as well as administrative controls and environmental designs that function…

  17. Leda and the Swan--and other myths about rape.

    PubMed

    Norfolk, Guy A

    2011-07-01

    In his David Jenkins Memorial Lecture, Dr Norfolk discusses rape and some of the myths that surround the topic, exploring the research evidence about conviction rates, false allegations, clinician gender preferences of rape victims and the role of sexual assault referral centres in service delivery. PMID:21663872

  18. Acceptance of Male Rape Myths among College Men and Women.

    ERIC Educational Resources Information Center

    Struckman-Johnson, Cindy; Struckman-Johnson, David

    1992-01-01

    College students (157 men and 158 women, mostly white middle-class) rated their agreement with statements reflecting myths about male rape. A majority disagree with all the myths, most strongly with the myth that rape is not traumatic for men. Results are discussed in terms of societal attitudes. (SLD)

  19. Using Education To Modify Students' Attitudes toward Rape.

    ERIC Educational Resources Information Center

    Jones, Jayme M.; Muehlenhard, Charlene

    The prevalence of rape points to a need for effective prevention strategies. Ultimately, however, preventing rape necessitates changing men's attitudes and behavior. This study investigated the effects of a lecture aimed at changing the attitudes of men. Also investigated were the effects of lecturer sex (male, female, or male-female team or no…

  20. Sex Differences in Perceptions and Punishment of Rape.

    ERIC Educational Resources Information Center

    Heim, Margaret; And Others

    Two studies examined the effects of variations of intent, outcome and pain in depictions of rape on the perceptions and punishment of rape. Numerous sex differences in perceptions were found. Females perceived more pain and tended to focus on the effects of outcome while males tended to respond more to the intent variation. Females assigned more…

  1. Models of Rape Judgment: Attributions Concerning Event, Perpetrator, and Victim.

    ERIC Educational Resources Information Center

    Langley, Travis; And Others

    1991-01-01

    Reviews models developed to identify cognitive mediators of rape judgments. Recent models examine perceptions of perpetrator's violent behavior and of victim's desire for intercourse as mediators of effects of violence levels and victim's behavior upon rape judgments. Sex differences in models suggest that female evaluators may be more likely to…

  2. "Spreading the Love" and Using Performances to Prevent Rape

    ERIC Educational Resources Information Center

    Murphy, Aurora

    2013-01-01

    While rape is ordinarily considered an invariable reality, Sharon Marcus argues that it is instead a cultural script, which casts women as victims and men as perpetrators. In imagining rape as a script, it may seem pertinent to suggest that the arts and performance have a role in its prevention. Performances can provide a forum to publicly reflect…

  3. Power, Sex, and Violence: The Case of Marital Rape

    ERIC Educational Resources Information Center

    Gelles, Richard J.

    1977-01-01

    The available evidence on marital violence indicates that a number of women are forced into having sexual relations with their husbands through intimidation or physical force. Although marital rape is not possible in a strict legal sense, some women are talking about and reporting incidents of marital rape. (Author)

  4. Female University Student and Staff Perceptions of Rape.

    ERIC Educational Resources Information Center

    Herman, Michele Harway; Sedlacek, William E.

    Female university student and staff perceptions of rape at the University of Maryland were examined with the aid of an anonymous questionnaire on attitudes and perceptions of rape. A randomly selected sample group included 100 female students and 25 female classified employees. Results showed a 64% return rate was achieved despite many follow-up…

  5. Perception of Raped Source and Use of Fact in Stories

    ERIC Educational Resources Information Center

    Lampley, Linda Lee; Shaw, Donald L.

    1977-01-01

    Finds that student journalists reacted differently to--and wrote different stories about--a woman news source who spoke about a rape crisis center, depending on whether they had been told that the woman was herself a rape victim. (GW)

  6. Rape Counseling: A Model for Sensitizing and Training Helpers

    ERIC Educational Resources Information Center

    Silver, Steven M.; Stonestreet, Susan D.

    1978-01-01

    A woman who is raped needs factual information concerning hospital and legal procedures and an emotional support system to help her focus her feelings into productive channels. This article describes a training and outreach program combining rape simulation, film, lecture, and experiential discussion to stimulate thought, action, and increased…

  7. Should We Whistle as We Shirk? What is Rape Prevention?

    ERIC Educational Resources Information Center

    Neely, Margery A.

    Different theories have been offered about why some men rape. The interactive theory of rape describes coercive elements in heterosexual relations resulting from faulty socialization or abuse in childhood. Prevention according to this theory would involve mental health specialists incorporating preventive strategies into parent training, guidance,…

  8. Acquaintance Rape on Campus: The Problem, the Victims, and Prevention.

    ERIC Educational Resources Information Center

    Neff, Laurie

    1988-01-01

    Asserts that, although providing educational programs and materials on acquaintance rape and prevention strategies is important, attention must also be given to victims. Discusses recognition of stress response pattern of victims suffering from Rape Trauma Syndrome and provision of appropriate referral and support services. Includes selected list…

  9. Date and Acquaintance Rape: Perceptions and Attitude Change Strategies.

    ERIC Educational Resources Information Center

    Harrison, Patrick J.; And Others

    1991-01-01

    Investigated the dimensions of university students' (n=96) attitudes toward date and acquaintance rape. Examined the effects of a program designed to change those attitudes. Found college men had substantially greater tendencies to blame the victim for date and acquaintance rape than did women. (Author/ABL)

  10. Acquaintance Rape on College Campuses: A Review of the Literature.

    ERIC Educational Resources Information Center

    Kier, Frederick J.

    Although research on acquaintance, or date rape, dates back to the late 1950's, only recently has the literature in this area focused on prevention. In addition, although research has shown that, conservatively, 1 out of 10 women on university campuses has been raped by someone with whom she is familiar, little appears to have been done about the…

  11. A Model Acquaintance Rape Prevention Program for Men.

    ERIC Educational Resources Information Center

    Berkowitz, Alan D.

    1994-01-01

    Describes the underlying philosophy, content and evaluation issues, and selection and training of facilitators for a model acquaintance rape prevention program for male students conducted at Hobart College. Provides guidelines to help other colleges and universities implement a model rape prevention program for men that uses small-group discussion…

  12. Date Violence and Rape among Adolescents: Associations with Psychological Health.

    ERIC Educational Resources Information Center

    Ackard, Diann M.; Neumark-Sztainer, Dianne

    This study assessed the prevalence of date violence and rape among adolescents in order to examine the associations between date violence and rape, and eating disorders and psychopathology. The study also attempted to determine if these associations remained significant after controlling for sociodemographic characteristics. A Minnesota…

  13. Rape nitrogen nutrition diagnosis using continuum-removed hyperspectral reflectance

    NASA Astrophysics Data System (ADS)

    Zhang, Xuehong; Tian, Qingjiu

    2008-12-01

    The hyperspectral reflectance for rape fresh leaves and data of chlorophyll and total nitrogen content were acquired in primary growth stages under different nitrogen levels in order to monitor rape status and diagnose nitrogen using remote sensing method. A new method was developed for estimating the nitrogen nutrition of rape using continuum-removed method, which generally used in spectral analysis on rock and mineral. Based on the continuum-removed treatment and the correlation between absorption feature parameters and total nitrogen content of fresh leaves, results show that reflectance at the visible region decreased with increasing in the nitrogen fertilization, and continuum-removed operation can magnify the subtle difference in spectral absorption characteristics arose from the nitrogen stress on rape. During the seeding stage, bud-emerging stage and flowering stage of rape, total area of absorption peak, area left of the absorption peak and area right of the absorption peak in 550-750 nm region increased with increasing in the nitrogen fertilization, but it was opposite for the area-normalized maximal absorption depth. The correlation analysis indicated that it is at seeding stage that the relation between absorption characteristics parameters and leaf total nitrogen was best close. The research demonstrated that continuum-removed method is a feasible method for quantificational evaluation of rape nitrogen nutrition, and the seeding stage of rape is the best stage for assessment of rape nitrogen nutrition based on absorption characteristics of fresh leaves.

  14. A Model for Police Assistance to Rape Victims.

    ERIC Educational Resources Information Center

    Merchant, Jerrold J.

    Noting that an increasing number of rape victims who seek alternatives to police procedures do so because of the insensitive behavior of the investigating police officer, this paper provides a model of interpersonal communication skills that will enable police officers to better relate to and support the victims of rape. The areas covered in the…

  15. Decreased interpretation of nonverbal cues in rape victims.

    PubMed

    Giannini, A J; Price, W A; Kniepple, J L

    The ability to receive nonverbal facial cues was tested in twelve female victims of multiple nonserial rapes and matched controls. Subjects attempted to interpret nonverbal messages transmitted by male and female senders who were covertly taped while involved in a gambling task. Rape victims had significantly decreased ability to interpret the nonverbal facial cues of both male and female senders. PMID:3557809

  16. Resources for Developing Acquaintance Rape Prevention Programs for Men.

    ERIC Educational Resources Information Center

    Earle, James P.; Nies, Charles T.

    1994-01-01

    Provides an annotated bibliography of videos and printed materials that may be used as educational tools in rape prevention programs. Focuses on sources that are aimed directly at men. Also outlines the use of consultants or lecturers as one of many resources in the construction and implementation of rape prevention programs. (KW)

  17. Rape: It Can't Happen to Me!

    ERIC Educational Resources Information Center

    Chu, W.; Fong-torres, S.

    1979-01-01

    Cultural factors such as the family system, communication patterns and institutional racism make the impact of rape on Asian women different from its impact on other women. In this article suggestions for avoiding a rape attack on the street and at home are offered. (PMR)

  18. Rape Myth Beliefs and Bystander Attitudes among Incoming College Students

    ERIC Educational Resources Information Center

    McMahon, Sarah

    2010-01-01

    Objective: The bystander approach to rape prevention is gaining popularity on college campuses, although research is limited. This study explored bystander attitudes and their relationship with rape myths in a sample of college students. Participants: Surveys from 2,338 incoming undergraduate students at a large, northeastern university were…

  19. Rape Survivors' Agency within the Legal and Medical Systems

    ERIC Educational Resources Information Center

    Greeson, Megan R.; Campbell, Rebecca

    2011-01-01

    Many rape survivors seek help from the legal and medical systems post-assault. Previous studies have examined how social system personnel treat survivors, but less attention has been paid to how survivors attempt to shape their interactions with these systems. The purpose of this qualitative study was to examine rape survivors' agency--the active…

  20. 77 FR 30871 - Implementing the Prison Rape Elimination Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-23

    ..., Washington, May 17, 2012 [FR Doc. 2012-12743 Filed 5-22-12; 2:15 pm] Billing code 3110-01-P ... May 23, 2012 Part VI The President Memorandum of May 17, 2012--Implementing the Prison Rape... 3-- #0;The President ] Memorandum of May 17, 2012 Implementing the Prison Rape Elimination...

  1. Multiple Perpetrator Rape: Naming an Offence and Initial Research Findings

    ERIC Educational Resources Information Center

    Horvath, Miranda Angel Helena; Kelly, Liz

    2009-01-01

    Multiple perpetrator rape presents a significant problem nationally and internationally. However, previous research is limited and findings are often contradictory. The details of 101 rape allegations recorded in a six-month period in a large police force in England were analysed. Findings are presented about case classification, victim and…

  2. Rape Prevention with College Men: Evaluating Risk Status

    ERIC Educational Resources Information Center

    Stephens, Kari A.; George, William H.

    2009-01-01

    This study evaluates the effectiveness of a theoretically based rape prevention intervention with college men who were at high or low risk to perpetrate sexually coercive behavior. Participants (N = 146) are randomly assigned to the intervention or control group. Outcomes include rape myth acceptance, victim empathy, attraction to sexual…

  3. Development and Validation of the Korean Rape Myth Acceptance Scale

    ERIC Educational Resources Information Center

    Oh, Euna; Neville, Helen

    2004-01-01

    The purpose of this investigation was to develop a culturally relevant rape myth acceptance scale for Koreans. Three studies on the Korean Rape Myth Acceptance Scale (KRMAS) with approximately 1,000 observations provide initial validity and reliability. Specifically, results from exploratory and confirmatory factor analyses support four subscales:…

  4. Silent Survivors: Rape Myth Acceptance in Incarcerated Women's Narratives of Disclosure and Reporting of Rape

    ERIC Educational Resources Information Center

    Heath, Nicole M.; Lynch, Shannon M.; Fritch, April M.; McArthur, Lyn N.; Smith, Shilo L.

    2011-01-01

    Prior research suggests that rape victims who do not disclose or report to the police give reasons including self-blame and fear of judgment; however, this research has not been conducted with incarcerated women. Female offenders are a unique population because they experience high rates of sexual assault prior to incarceration. This study…

  5. The Role of the New "Date Rape Drugs" in Attributions about Date Rape

    ERIC Educational Resources Information Center

    Girard, April L.; Senn, Charlene Y.

    2008-01-01

    This study investigates the effect of voluntary and involuntary drug use on attributions about sexual assault. The sample was composed of 280 randomly selected male and female undergraduate students. The type of drug used (GHB, alcohol, or none) and the voluntariness of the administration were varied in an unambiguous date rape scenario.…

  6. Risk factors for rape re-victimisation: a retrospective analysis.

    PubMed

    Lurie, S; Boaz, M; Golan, A

    2013-11-01

    Sexual re-victimisation refers to a pattern in which the sexual assault victim has an increased risk of subsequent victimisation relative to an individual who was never victimised. The purpose of our study was to identify risks factors for a second rape, the severest form of sexual re-victimisation. All rape victims treated at the First Regional Israeli Center for Sexual Assault Victims between October 2000 and July 2010 were included in this retrospective analysis. We compared characteristics of 53 rape victims who were victimised twice to those of 1,939 rape victims who were victimised once. We identified several risk factors for a second rape, which can be used in prevention programmes. These are: psychiatric background, history of social services involvement, adulthood, non-virginity and minority ethnicity. PMID:24219731

  7. Evaluation of an intervention to change attitudes toward date rape.

    PubMed

    Lanier, C A; Elliott, M N; Martin, D W; Kapadia, A

    1998-01-01

    The prevalence of date rape among college students is a major concern. Although much research has been done on risk factors for date rape, few researchers have specifically described interventions for the various stages of developing a date-rape prevention program. Previous programs have often relied on educational videos that feature a "typical" date-rape scenario, a format that some researchers suggest may have a negative effect on the way people engage in aggressive sexual behavior. A less violent theatrical production based on social learning theory and risk-factor reduction that resulted in a significant improvement in attitudes related to date rape among both male and female students at an elite Texas university is described. PMID:9519580

  8. Attitudes toward Rape: Gender and Ethnic Differences across Asian and Caucasian College Students.

    ERIC Educational Resources Information Center

    Mori, Lisa; And Others

    1995-01-01

    Examined 302 Asian and Caucasian college students' attitudes towards rape victims and belief in rape myths. Results show significant differences between both groups. Asians more often endorsed negative attitudes toward rape victims than whites, and males endorsed more negative attitudes and more acceptance of rape myths than females. Results are…

  9. A Preliminary Study of Eighth Grade Students' Attitudes toward Rape Myths and Women's Roles.

    ERIC Educational Resources Information Center

    Boxley, Jeanne; And Others

    1995-01-01

    This study examined the relationship between sex role stereotypes of women and eighth graders' beliefs in rape myths. Surveys indicated that both sexes accepted some rape myths and sex role stereotyping of women. There was an association between belief in rape myths and stereotyping. More boys than girls accepted rape myths. (SM)

  10. Date Rape: Its Relationship to Trauma Symptoms and Sexual Self-Esteem.

    ERIC Educational Resources Information Center

    Shapiro, Brenda L.; Schwarz, J. Conrad

    1997-01-01

    Extends previous research on date rape by assessing trauma symptoms and sexual self-esteem among college women who had, or who had not, been raped. Results indicate that rape victims had significantly more trauma symptoms and lower sexual self-esteem compared to other women, thus suggesting date rape's significant consequences. (RJM)

  11. Scripts for a Date Rape: Similarities and Differences in Males and Females Scripts.

    ERIC Educational Resources Information Center

    Clark, M. Diane; And Others

    While the issue of rape evokes strong responses, confusion seems to occur not when a blitz or stranger rape occurs, but when an acquaintance or date rape occurs. This study assessed acquaintance rape scripts given a preset scenario. Subjects included female college students (N=32) and male college students (N=19). Subjects were given a scenario…

  12. 66 FR 43262 - Grants for Rape Prevention and Education; Notice of Availability of Funds

    Federal Register 2010, 2011, 2012, 2013, 2014

    2001-08-17

    ... HUMAN SERVICES Centers for Disease Control and Prevention Grants for Rape Prevention and Education..., to support programs addressing violence against women. The Rape Prevention and Education Grant... States and Territories to be used for rape prevention and education programs conducted by rape...

  13. Rape and Child Sexual Abuse: What Beliefs Persist about Motives, Perpetrators, and Survivors?

    ERIC Educational Resources Information Center

    McGee, Hannah; O'Higgins, Madeleine; Garavan, Rebecca; Conroy, Ronan

    2011-01-01

    Rape myths are prejudicial and stereotyped beliefs about rape which persist in society. They may have a significant impact on those affected by rape as well as the performance of legal and public participants in the justice system. Rape myths may differ over time and within different societies and cultural settings. Awareness of contemporary and…

  14. [The knowledge about "date rape drugs" among Polish students].

    PubMed

    Dziuban', Agata; Ratecka, Anna; Brzyski, Piotr; Foryś, Zofia; Gomółka, Ewa; Ogonowska, Dorota

    2009-01-01

    The Main Department of Police in Poland notes about 2000 rapes a year. Some of the crimes are performed with "Date Rape Drugs". The term means substances helping comitting a rape such as GHB (gamma hydroxybutyric acid), ketamine, flunitrazepam and other benzodiazepines derivatives, MDMA ("ecstasy"), marihuana, amphetamine. The substances are often joined with alcohol. The victims are usually young women, and not all the cases are recorded by the police or physicians, because the victims often do not remember details of the event. The toxicological analysis of blood or urine would be helpful to explain the circumstances of the case and to prove using "Date Rape Drug". The samples for toxicological determinations should be collected as soon as possible (24 to 72 hours after admission). Preventing violence with "Date Rape Drugs" include wide education by media, police, teachers and parents. The purpose of the research was to check the level of knowledge about "Date Rape Drugs". The consciousness of risk behavior when the kind of substances is used and the ways of preventing the risk of being a sexual victim were checked. Material for the research were the results of questionnaire prepared by The Department of Medicine Sociology Collegium Medicum Jagiellonian University in Krakow, carried out on 740 students. Most of respondents (77%) were women. The age of respondents was between 19-36 years (mean 21.41; SD - 1.29). The results of the research showed, that respondents didn't have completed knowledge about "Date Rape Drugs". They did not know the ways of recognizing and preventing the risk of being given this kind of substances. The main source of information about "Date Rape Drugs" were internet and colleagues. There is a need to start education about "Date Rape Drugs" by serious institutions such as the police and schools in Poland. This is the best way to prevent young people against a risk of being given "Date Rape Drugs" and being a victim of sexual crimes. PMID

  15. Evaluation of a University-based Date Rape Prevention Program: Effect on Attitudes and Behavior Related to Rape.

    ERIC Educational Resources Information Center

    Shultz, Shelli K.; Scherman, Avraham; Marshall, Lawrence J.

    2000-01-01

    Investigates the effectiveness of a date rape prevention program for both male and female undergraduate students (N=60) in changing attitudes and behavioral intent, and exploring the attitude-behavior link for both genders. Results reveal that participants in the treatment groups were less accepting of rape myths and endorsed attitudes…

  16. Gender, Gender Identity, Rape Myth Acceptance, and Time of Initial Resistance on the Perception of Acquaintance Rape Blame and Avoidability.

    ERIC Educational Resources Information Center

    Kopper, Beverly A.

    1996-01-01

    Investigated the role of gender, gender role identity, rape myth acceptance, and initial resistance in assigning blame in the case of acquaintance rape, and studied the perceived avoidability of the assault for 355 female and 179 male college students. The gender differences in responses are discussed. (SLD)

  17. "You Owe Me": Effects of Date Cost, Who Pays, Participant Gender, and Rape Myth Beliefs on Perceptions of Rape

    ERIC Educational Resources Information Center

    Basow, Susan A.; Minieri, Alexandra

    2011-01-01

    Sexual social exchange theory was applied to perceptions of a date rape by manipulating the cost of the date and who paid in vignettes presented to 188 U.S. college students, who then rated the characters' sexual expectations, blame, responsibility, and rape justifiability. Findings from this between-participant design partially supported…

  18. Perceptions of, and Assistance Provided to, a Hypothetical Rape Victim: Differences Between Rape Disclosure Recipients and Nonrecipients

    PubMed Central

    Paul, Lisa A.; Kehn, Andre; Gray, Matt J.; Salapska-Gelleri, Joanna

    2014-01-01

    Objective Undergraduate rape disclosure recipients and nonrecipients’ sociodemographic and life experience variables, attitudes towards rape and responses to a hypothetical rape disclosure were compared to determine differences between them. Participants One-hundred-ninety-two undergraduates at three universities participated in this online survey between November 2011 – April 2012. Methods Participants reported on their rape myth acceptance (RMA) and personal direct and indirect (i.e., disclosure receipt) experiences with sexual assault. Participants also responded to a hypothetical rape disclosure. Results Disclosure recipients were more likely to report a victimization history, and less confusion and perceived ineffectiveness in helping the hypothetical victim. RMA and nonrecipient status predicted perceived victim responsibility; these variables and childhood victimization predicted confusion about helping. RMA also predicted perceived ineffectiveness of one’s helping behaviors. Victimization history and female gender predicted victim empathy. Conclusions These findings can inform sexual assault-related programming for undergraduates through the provision of targeted assistance and corrective information. PMID:24779405

  19. Mental health assessment of rape offenders

    PubMed Central

    Sarkar, Jaydip

    2013-01-01

    There is an urgent need for development of methods of assessment and management of sex offenders (rapists, child sex offenders, other sexual offenders, and murderers) to mount a society-wide battle against the scourge of sexual offences in India. This paper provides an overview of theories, models, and assessment methods of rapists. It draws upon literature from psychiatry, psychology, criminology, probation, and ethics to provide a framework for understanding reasons behind rape, how mental health issues are implicated, what mental health professionals can do to contribute to crime management, and why this is ethically right and proper. PMID:24082243

  20. Receipt of Post-Rape Medical Care in a National Sample of Female Victims

    PubMed Central

    Zinzow, Heidi M.; Resnick, Heidi S.; Barr, Simone C.; Danielson, Carla K.; Kilpatrick, Dean G.

    2014-01-01

    Background It is important for rape victims to receive medical care to prevent and treat rape-related diseases and injuries, access forensic exams, and connect to needed resources. Few victims seek care, and factors associated with post-rape medical care–seeking are poorly understood. Purpose The current study examined prevalence and factors associated with post-rape medical care–seeking in a national sample of women who reported a most-recent or only incident of forcible rape, and drug- or alcohol-facilitated/incapacitated rape when they were aged ≥14 years. Methods A national sample of U.S. adult women (N=3001) completed structured telephone interviews in 2006, and data for this study were analyzed in 2011. Logistic regression analyses examined demographic variables, health, rape characteristics, and post-rape concerns in relation to post-rape medical care–seeking among 445 female rape victims. Results A minority of rape victims (21%) sought post-rape medical attention following the incident. In the final multivariate model, correlates of medical care included black race, rape-related injury, concerns about sexually transmitted diseases, pregnancy concerns, and reporting the incident to police. Conclusions Women who experience rapes consistent with stereotypic scenarios, acknowledge the rape, report the rape, and harbor health concerns appear to be more likely to seek post-rape medical services. Education is needed to increase rape acknowledgment, awareness of post-rape services that do not require formal reporting, and recognition of the need to treat rape-related health problems. PMID:22813683

  1. Acquaintance rape and the high school student.

    PubMed

    Davis, T C; Peck, G Q; Storment, J M

    1993-05-01

    The purpose of this study was to determine the prevalence of forced sex among high school students and to assess student attitudes about it. The questionnaires were given in conjunction with a presentation on date rape to 9th through 12th grade students in health and science classes in three large metropolitan Louisiana public high schools. The first questionnaire, given before the presentation, elicited demographic information and attitudes about forced sex in 11 scenarios; the second, given after the program, asked if the student had ever been in a situation involving forced sex and, if so, whether he or she had told anyone. The two questionnaires were completed by all 237 students. Although one out of five students (20%) reported they had experienced forced sex, only half had told about the experience. A majority of boys (60%) found it acceptable in one or more situations for a boy to force sex on a girl. Male, black, and older students were significantly more likely (p < 0.05) to indicate that it was acceptable for a boy to force sex on a girl in certain circumstances. These findings should alert physicians to the risk of acquaintance rape among their high school student patients. Student attitudes, particularly those of males, may provide an important focus of preventive sex education in the physician's office, the classroom, and the community. PMID:8323934

  2. Rape in war: the humanitarian response

    PubMed Central

    Shanks, Leslie; Schull, Michael J.

    2000-01-01

    Women and children are vulnerable to sexual violence in times of conflict, and the risk persists even after they have escaped the conflict area. The impact of rape goes far beyond the immediate effects of the physical attack and has long-lasting consequences. We describe the humanitarian community's response to sexual violence and rape in times of war and civil unrest by drawing on the experiences of Médecins Sans Frontières/Doctors Without Borders and other humanitarian agencies. Health care workers must have a keen awareness of the problem and be prepared to respond appropriately. This requires a comprehensive intervention protocol, including antibiotic prophylaxis, emergency contraception, referral for psychological support, and proper documentation and reporting procedures. Preventing widespread sexual violence requires increasing the security in refugee camps. It also requires speaking out and holding states accountable when violations of international law occur. The challenge is to remain alert to these often hidden, but extremely destructive, crimes in the midst of a chaotic emergency relief setting. PMID:11079062

  3. Impact of rape on female sexuality: review of selected literature.

    PubMed

    Weaver, Terri L

    2009-12-01

    This article reviews the medical and psychologic literature on the impact of rape on female sexuality. Studies documented moderately high rates of genital injuries and moderate rates of sexually transmitted infections postrape and significantly greater difficulties with aspects of reproductive/sexual functioning, including dyspareunia, endometriosis, menstrual irregularities, and chronic pelvic pain for raped compared with nonraped women. Raped women also engaged in significantly more high-risk sexual behaviors. Posttraumatic stress disorder emerged as an important mediator of sexual victimization and sexual health. An integrative model outlining interrelated physical, psychologic, biologic, and behavioral factors postrape was presented to assimilate review findings. PMID:20393422

  4. College women's experiences with rape disclosure: a national study.

    PubMed

    Paul, Lisa A; Walsh, Kate; McCauley, Jenna L; Ruggiero, Kenneth J; Resnick, Heidi S; Kilpatrick, Dean G

    2013-04-01

    Disclosure of a rape to informal support sources (e.g., friends) is a relatively common experience, but it is not well understood. This study expands our limited knowledge of the characteristics and life experiences of disclosure recipients among a national sample of 2,000 female college students. Over 40% of respondents reported having received a rape disclosure, and more than two thirds of these recipients encouraged victims to formally report their rapes to the police or other authorities. Correlates of disclosure receipt and encouragement of reporting, including personal assault history, mental health history, and substance use, are presented and discussed. PMID:23651638

  5. The effects of receiving a rape disclosure: college friends' stories.

    PubMed

    Branch, Kathryn A; Richards, Tara N

    2013-05-01

    Research suggests that college women are at greater risk for rape and sexual assault than women in the general population or in a comparable age group. College women, away from home and family, may turn to friends for support. Friends may experience emotional reactions that affect their own functioning and may not feel they have anywhere to turn. In this study, we interviewed male and female college students who had received a rape disclosure from a friend. Their unique stories provide insight into the secondary effects of rape disclosure on friends. Implications of these findings for college campuses are discussed. PMID:23743351

  6. Transfer of thallium from rape seed to rape oil is negligible and oil is fit for human consumption.

    PubMed

    Loula, Martin; Kaňa, Antonín; Vosmanská, Magda; Koplík, Richard; Mestek, Oto

    2016-04-01

    Rape and other Brassicaceae family plants can accumulate appreciable amounts of thallium from the soil. Because some species of this family are common crops utilised as food for direct consumption or raw materials for food production, thallium can enter the food chain. A useful method for thallium determination is inductively coupled plasma mass spectrometry. The limit of detection (0.2 pg ml(-1) Tl or 0.02 ng g(-1) Tl, taking in the account dilution during sample decomposition) found in the current study was very low, and the method can be used for ultra-trace analysis. Possible transfer of thallium from rape seed to the rape oil was investigated in two ways. The balance of thallium in rape seed meal (content 140-200 ng g(-1) Tl) and defatted rape seed meal indicated that thallium did not pass into the oil (p < 0.05). Moreover, the analyses of thallium in six kinds of edible rape seed oil and three kinds of margarines showed that the amount of thallium in rape seed oil is negligible. PMID:26934111

  7. Expression analysis of a novel pyridoxal kinase messenger RNA splice variant, PKL, in oil rape suffering abiotic stress and phytohormones.

    PubMed

    Yu, Shunwu; Luo, Lijun

    2008-12-01

    Pyridoxal kinase is key enzyme for the biosynthesis of pyridoxal 5'-phosphate, the biologically active form of vitamin B6, in the salvage pathway. A pyridoxal kinase gene, BnPKL (GenBank accession No. DQ463962), was isolated from oilseed rape (Brassica napus L.) following water stress through rapid amplification of complementary DNA (cDNA) ends. The results showed that the gene had two splice variants: PKL and PKL2. PKL, the long cDNA, encodes a 334 amino acid protein with a complete ATP-binding site, pyridoxal kinase-binding site and dimer interface site of a pyridoxal kinase, while PKL2, the short cDNA, lacked a partial domain. Southern blot showed that there were two copies in Brassica napus. The expression of BnPKL cDNA could rescue the mutant phenotype of Escherichia coli defective in pyridoxal kinase. Real-time reverse transcription-polymerase chain reaction revealed that the relative abundance of two transcripts are modulated by development and environmental stresses. Abscisic acid and NaCl were inclined to decrease PKL expression, but H2O2 and cold temperatures induced the PKL expression. In addition, the PKL expression could be transiently induced by jasmonate acid at an early stage, abscisic acid, salicylic acid and jasmonate acid enhanced the PKL expression in roots. Our results demonstrated that BnPKL was a pyridoxal kinase involved in responses to biotic and abiotic stresses. PMID:19089298

  8. Draft genome sequence of the oilseed species Ricinus communis.

    PubMed

    Chan, Agnes P; Crabtree, Jonathan; Zhao, Qi; Lorenzi, Hernan; Orvis, Joshua; Puiu, Daniela; Melake-Berhan, Admasu; Jones, Kristine M; Redman, Julia; Chen, Grace; Cahoon, Edgar B; Gedil, Melaku; Stanke, Mario; Haas, Brian J; Wortman, Jennifer R; Fraser-Liggett, Claire M; Ravel, Jacques; Rabinowicz, Pablo D

    2010-09-01

    Castor bean (Ricinus communis) is an oilseed crop that belongs to the spurge (Euphorbiaceae) family, which comprises approximately 6,300 species that include cassava (Manihot esculenta), rubber tree (Hevea brasiliensis) and physic nut (Jatropha curcas). It is primarily of economic interest as a source of castor oil, used for the production of high-quality lubricants because of its high proportion of the unusual fatty acid ricinoleic acid. However, castor bean genomics is also relevant to biosecurity as the seeds contain high levels of ricin, a highly toxic, ribosome-inactivating protein. Here we report the draft genome sequence of castor bean (4.6-fold coverage), the first for a member of the Euphorbiaceae. Whereas most of the key genes involved in oil synthesis and turnover are single copy, the number of members of the ricin gene family is larger than previously thought. Comparative genomics analysis suggests the presence of an ancient hexaploidization event that is conserved across the dicotyledonous lineage. PMID:20729833

  9. Flowering dynamics and pollinator visitation of oilseed echium (Echium plantagineum).

    PubMed

    Eberle, Carrie A; Forcella, Frank; Gesch, Russ; Weyers, Sharon; Peterson, Dean; Eklund, James

    2014-01-01

    Echium (Echium plantagineum L.) is an alternative oilseed crop in summer-wet temperate regions that provides floral resources to pollinators. Its seed oil is rich in omega-3 fatty acids, such as stearidonic acid, which is desired highly by the cosmetic industry. Seeds were sown in field plots over three years in western Minnesota in spring (early-sown) or early summer (late-sown), and flower abundance, pollinator visitation, and seed yields were studied. Initial flowering commenced 41 to 55 d after sowing, and anthesis duration (first flowering to harvest) was 34 to 70 d. Late sowing dates delayed anthesis, but increased the intensity of visitation by pollinators. Cumulative flower densities ranged from 1 to 4.5 billion ha-1. Flowers attracted numerous honey bees (Apis mellifera L.), as many as 35 per minute of observation, which represented about 50% of all insect visitors. Early-sown echium produced seed yields up to 750 kg ha-1, which were 2-29 times higher than those of late-sown echium. Early sowing of echium in Minnesota provides abundant floral resources for pollinators for up to two months and simultaneously produces seed yields whose profits rival those of corn (Zea mays L.). PMID:25427071

  10. Flowering Dynamics and Pollinator Visitation of Oilseed Echium (Echium plantagineum)

    PubMed Central

    Eberle, Carrie A.; Forcella, Frank; Gesch, Russ; Weyers, Sharon; Peterson, Dean; Eklund, James

    2014-01-01

    Echium (Echium plantagineum L.) is an alternative oilseed crop in summer-wet temperate regions that provides floral resources to pollinators. Its seed oil is rich in omega-3 fatty acids, such as stearidonic acid, which is desired highly by the cosmetic industry. Seeds were sown in field plots over three years in western Minnesota in spring (early-sown) or early summer (late-sown), and flower abundance, pollinator visitation, and seed yields were studied. Initial flowering commenced 41 to 55 d after sowing, and anthesis duration (first flowering to harvest) was 34 to 70 d. Late sowing dates delayed anthesis, but increased the intensity of visitation by pollinators. Cumulative flower densities ranged from 1 to 4.5 billion ha−1. Flowers attracted numerous honey bees (Apis mellifera L.), as many as 35 per minute of observation, which represented about 50% of all insect visitors. Early-sown echium produced seed yields up to 750 kg ha−1, which were 2–29 times higher than those of late-sown echium. Early sowing of echium in Minnesota provides abundant floral resources for pollinators for up to two months and simultaneously produces seed yields whose profits rival those of corn (Zea mays L.). PMID:25427071

  11. Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production.

    PubMed

    Liu, Fushan; Zhao, Qianru; Mano, Noel; Ahmed, Zaheer; Nitschke, Felix; Cai, Yinqqi; Chapman, Kent D; Steup, Martin; Tetlow, Ian J; Emes, Michael J

    2016-03-01

    We have identified a novel means to achieve substantially increased vegetative biomass and oilseed production in the model plant Arabidopsis thaliana. Endogenous isoforms of starch branching enzyme (SBE) were substituted by either one of the endosperm-expressed maize (Zea mays L.) branching isozymes, ZmSBEI or ZmSBEIIb. Transformants were compared with the starch-free background and with the wild-type plants. Each of the maize-derived SBEs restored starch biosynthesis but both morphology and structure of starch particles were altered. Altered starch metabolism in the transformants is associated with enhanced biomass formation and more-than-trebled oilseed production while maintaining seed oil quality. Enhanced oilseed production is primarily due to an increased number of siliques per plant whereas oil content and seed number per silique are essentially unchanged or even modestly decreased. Introduction of cereal starch branching isozymes into oilseed plants represents a potentially useful strategy to increase biomass and oilseed production in related crops and manipulate the structure and properties of leaf starch. PMID:26285603

  12. Changing attitudes and perceptions of Hispanic men ages 18 to 25 about rape and rape prevention.

    PubMed

    Lawson, Sarah Lynn; Munoz-Rojas, Derby; Gutman, Lauren Samantha; Siman, Matilde Nathalia

    2012-12-01

    This exploratory study was designed to test the effectiveness of Foubert's intervention, The Men's Program, on a sample of 18- to 25-year-old Hispanic men who are not enrolled at a University or College. We explored participants' perceptions of, reactions to, and experience of the program. The information collected will be used to adapt the program to this specific population in order to increase cultural relevancy and specificity and create lasting attitudinal change. Three groups of six-eight Hispanic males (n = 22) were exposed to the intervention program. The Bystander Attitude Scale and the Rape Attitude and Beliefs Scale were administered as pre- and post-test measures. A short focus group was conducted to ask the men about their experience of the intervention. Analysis showed a significant increase in participants' willingness to intervene (p = 0.005) along with a decrease in rape myth acceptance in four of the five subscales (Justice p = 0.03; Status p = 0.004; Tactics p = 0.04; and Gender p = 0.002) after exposure to the intervention. Analysis of focus group material yielded several interesting themes about knowledge of rape, family and culture, perceptions of women, and the program material. This study showed promising change in attitudes about rape beliefs and bystander behaviors in Hispanic males exposed to an educational intervention. Through the information obtained, a cultural adaption from the analysis of the focus group data will be implemented during Phase 2 of the study. The adapted intervention will be tested before, after, and 1- and 3-months post-intervention to test whether the change in attitudes and behaviors are sustainable over time. PMID:23215988

  13. Crop yield and quality, weeds, insects, and water use of durum and selected brassicaceae oilseeds in two-year rotations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cool-season oilseeds are potential feedstock for biofuel production, but few studies have compared oilseed-durum (Triticum durum Desf.) rotations. We conducted a field trial under dryland conditions for 2007-2010 near Froid, Montana, comparing productivity, water balance, and key weed and arthropod...

  14. Assessment of Rape-Supportive Attitudes and Beliefs in College Men: Development, Reliability, and Validity of the Rape Attitudes and Beliefs Scale

    ERIC Educational Resources Information Center

    Burgess, Gerald H.

    2007-01-01

    Discussed is the development and psychometric analysis of a measure of rape-supportive attitudes and beliefs called the Rape Attitudes and Beliefs Scale (RABS), intended for the use with college men. Items were developed from a literature review of "rape myths" that were correlated to some measure of sexual aggression. An exploratory factor…

  15. Schema Effects of Rape Myth Acceptance on Judgments of Guilt and Blame in Rape Cases: The Role of Perceived Entitlement to Judge

    ERIC Educational Resources Information Center

    Eyssel, Friederike; Bohner, Gerd

    2011-01-01

    Two experiments (N = 330) examined conditions that facilitate biasing effects of rape myth acceptance (RMA) on judgments of blame in rape cases. In both experiments, participants read a short vignette depicting a rape case. In Experiment 1, the amount of case-irrelevant information about defendant and plaintiff was varied. As predicted, high-RMA…

  16. Current perceptions of marital rape: some good and not-so-good news.

    PubMed

    Ferro, Christine; Cermele, Jill; Saltzman, Ann

    2008-06-01

    The current study extends previous research on marital rape and acceptance of general rape myths by comparing the perceptions of undergraduate college students (n = 85) to those of college alumni/ae (n = 44) who graduated from the same university three decades earlier. Participants read a hypothetical rape scenario that depicted the perpetrator as either the victim's husband or neighbor and completed three measures of different aspects of rape myth acceptance. Results indicated that although participants reported fairly low levels of support for different aspects of rape myths, certain rape myths were more strongly endorsed than were others. Furthermore, rape myth acceptance was stronger for marital rape than for acquaintance rape and for undergraduates than for college alumni/ae. Practical and theoretical implications of this study are discussed. PMID:18272725

  17. Rape myth acceptance, sexual trauma history, and posttraumatic stress disorder.

    PubMed

    Baugher, Shannon N; Elhai, Jon D; Monroe, James R; Gray, Matt J

    2010-11-01

    The prediction of false rape-related beliefs (rape myth acceptance [RMA]) was examined using the Illinois Rape Myth Acceptance Scale (Payne, Lonsway, & Fitzgerald, 1999) among a nonclinical sample of 258 male and female college students. Predictor variables included measures of attitudes toward women, gender role identity (GRI), sexual trauma history, and posttraumatic stress disorder (PTSD) symptom severity. Using linear regression and testing interaction effects, negative attitudes toward women significantly predicted greater RMA for individuals without a sexual trauma history. However, neither attitudes toward women nor GRI were significant predictors of RMA for individuals with a sexual trauma history. PTSD did not moderate RMA's relationship with attitudes toward women and GRI. This study has clinical implications for treatment as well as for the development of rape myth-dispelling programs. PMID:20065314

  18. 42 CFR 50.306 - Rape and incest.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... APPLICABILITY Abortions and Related Medical Services in Federally Assisted Programs of the Public Health Service... participation is also available in expenditures for abortions for victims of rape or incest under...

  19. 42 CFR 50.306 - Rape and incest.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... APPLICABILITY Abortions and Related Medical Services in Federally Assisted Programs of the Public Health Service... participation is also available in expenditures for abortions for victims of rape or incest under...

  20. 42 CFR 50.306 - Rape and incest.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... APPLICABILITY Abortions and Related Medical Services in Federally Assisted Programs of the Public Health Service... participation is also available in expenditures for abortions for victims of rape or incest under...

  1. 42 CFR 50.306 - Rape and incest.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... APPLICABILITY Abortions and Related Medical Services in Federally Assisted Programs of the Public Health Service... participation is also available in expenditures for abortions for victims of rape or incest under...

  2. 42 CFR 50.306 - Rape and incest.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... APPLICABILITY Abortions and Related Medical Services in Federally Assisted Programs of the Public Health Service... participation is also available in expenditures for abortions for victims of rape or incest under...

  3. World oilseed situation and US export opportunities, June 1982. Foreign agriculture circular

    SciTech Connect

    Not Available

    1982-06-01

    World oilseed output for 1982/83 is forecast at 175 million tons, unchanged from May, but up 2.2 million tons from 1981/82. World soybean production for 1981/82 remains unchanged from last month's estimate. Export estimates for soybeans for both Argentina and the United States were adjusted upward to reflect recent activity. U.S. exports of soybeans for 1981/82 are forecast at 24.5 million tons, 3 percent above the record in 1979/80. Spain has tendered for at least 20,000 tons of sunflowerseed oil and could possibly be in the market for sunflowerseed in the near future. Mexico's increasing use of oilseeds and reduced safflower and cottonseed production has also stimulated significant imports of U.S. sunflowerseed. Trade barriers and policies: Japan announced various measures to liberalize imports in May. These include tariff reductions on 4 oilseed products.

  4. Food potentials of some unconventional oilseeds grown in Nigeria--a brief review.

    PubMed

    Badifu, G I

    1993-05-01

    A brief review of literature on kernels of Citrullus and Cucumeropsis ('egusi' melon) species, Telfairia occidentalis (fluted pumpkin), Lagenaria (gourd) species of all of Cucurbitaceae family and other oilseeds such as Pentaclethra macrophylla (African oil bean), Parkia spp. (African locust bean) both of Mimosaceae family and Butyrospermum paradoxum (shea butter) of Sapotaceae family which are grown and widely used as food in Nigeria is presented. The kernels of species of Cucurbitaceae form the bulk of unconventional oilseeds used for food in Nigeria. The nutritional value of some of the kernels and the physicochemical properties and storage stability of the oils obtained from them are discussed. The various consumable forms in which they exist are also described. The problems and prospects of these neglected oilseeds in Nigeria are highlighted. PMID:8506236

  5. Understanding Disparities in Service Seeking Following Forcible Versus Drug- or Alcohol-Facilitated/Incapacitated Rape.

    PubMed

    Walsh, Kate; Zinzow, Heidi M; Badour, Christal L; Ruggiero, Kenneth J; Kilpatrick, Dean G; Resnick, Heidi S

    2016-09-01

    Victims of drug- or alcohol-facilitated/incapacitated rape (DAFR/IR) are substantially less likely to seek medical, rape crisis, or police services compared with victims of forcible rape (FR); however, reasons for these disparities are poorly understood. The current study examined explanatory mechanisms in the pathway from rape type (FR vs. DAFR/IR) to disparities in post-rape service seeking (medical, rape crisis, criminal justice). Participants were 445 adult women from a nationally representative household probability sample who had experienced FR, DAFR/IR, or both since age 14. Personal characteristics (age, race, income, prior rape history), rape characteristics (fear, injury, loss of consciousness), and post-rape acknowledgment, medical concerns, and service seeking were collected. An indirect effects model using bootstrapped standard errors was estimated to examine pathways from rape type to service seeking. DAFR/IR-only victims were less likely to seek services compared with FR victims despite similar post-rape medical concerns. FR victims were more likely to report fear during the rape and a prior rape history, and to acknowledge the incident as rape; each of these characteristics was positively associated with service seeking. However, only prior rape history and acknowledgment served as indirect paths to service seeking; acknowledgment was the strongest predictor of service seeking. Diminished acknowledgment of the incident as rape may be especially important to explaining why DAFR/IR victims are less likely than FR victims to seek services. Public service campaigns designed to increase awareness of rape definitions, particularly around DAFR/IR, are important to reducing disparities in rape-related service seeking. PMID:25846758

  6. [Anxiety and depression levels in rape offenders].

    PubMed

    Herrera-Hernández, E; Marván, L; Saavedra, M; Contreras, C M

    1993-03-01

    Whether the active subject of rape possesses a particular personality trait is a much debated matter. It is an accepted fact that a small amount of rapists fall into the sadistic diagnostic category. Other psychopatologic traits likely to identify a rapist are, however, unknown. The present study was carried over at a jail near the city of Xalapa (Veracruz) Mexico. Hamilton, IDARE, and Zung scales, in their modified Spanish version were applied to a sample of imprisoned adult rapists. Compared to their control group (men serving sentences for other felonies), rapists scored higher in anxiety, and depression scales. In the light of these results, it is suggested that some rapists are likely to be anxious-depressive subjects that, after having "acquired" a sociopathic repertoire, offend other people in conditions of indefensiveness, contrary to depressive subjects who aggress themselves. PMID:8237434

  7. Women's erotic rape fantasies: an evaluation of theory and research.

    PubMed

    Critelli, Joseph W; Bivona, Jenny M

    2008-01-01

    This article is the first systematic review of the research literature on women's rape fantasies. Current research indicates that between 31% and 57% of women have fantasies in which they are forced into sex against their will, and for 9% to 17% of women these are a frequent or favorite fantasy experience. Erotic rape fantasies are paradoxical: they do not appear to make sense. Why would a person have an erotic and pleasurable fantasy about an event that, in real life, would be abhorrent and traumatic? In this article, the major theories of women's rape fantasies are evaluated both rationally and empirically. These theories explain rape fantasies in terms of masochism, sexual blame avoidance, openness to sexuality, sexual desirability, male rape culture, biological predisposition to surrender, sympathetic physiological activation, and adversary transformation. This article evaluates theory and research, makes provisional judgments as to which theories appear to be most viable, and begins the task of theoretical integration to arrive at a more complete and internally consistent explanation for why many women engage in erotic rape fantasies. Methodological critiques and programs for future research are presented throughout. PMID:18321031

  8. "You owe me": effects of date cost, who pays, participant gender, and rape myth beliefs on perceptions of rape.

    PubMed

    Basow, Susan A; Minieri, Alexandra

    2011-02-01

    Sexual social exchange theory was applied to perceptions of a date rape by manipulating the cost of the date and who paid in vignettes presented to 188 U.S. college students, who then rated the characters' sexual expectations, blame, responsibility, and rape justifiability. Findings from this between-participant design partially supported predictions: When the man paid for an expensive date, men agreed more than did women that both characters should have expected sexual intercourse. Conversely, when the costs of an inexpensive date were split, the perpetrator was assigned the most blame, and women agreed more than men that no sexual expectations were warranted. Participant gender affected some responses, although level of rape myth acceptance was the main predictor of rape perceptions. PMID:20442451

  9. World oilseed situation and U. S. export opportunities, March 1984. Foreign agriculture circular

    SciTech Connect

    Not Available

    1984-03-01

    The world oilseed supply situation tightened in this month with estimated world oilseed, meal and oil production all down from the last month. Reduced peanut and sunflower seed output in drought-damaged areas in Africa more than offset an increase in Argentine soybean output. The protein meal sector remained quiet, with an adjustment in peanut meal production and a reduction in Soviet soybean meal imports. The situation for vegetable and marine oils also remained quiet during the month, with offsetting reductions of Singapore's palm oil imports and exports indicating that country's decline as a transshipper of palm oil.

  10. Metacognitive aspects of rape myths: subjective strength of rape myth acceptance moderates its effects on information processing and behavioral intentions.

    PubMed

    Süssenbach, Philipp; Eyssel, Friederike; Bohner, Gerd

    2013-07-01

    The authors present a metacognitive approach to influences of rape myth acceptance (RMA) on the processing of rape-related information and rape proclivity. In Study 1, participants (N = 264) completed an RMA scale and subsequently reported the subjective strength (e.g., importance, certainty) of their RMA. Then they read about a rape case, viewed a photograph of the alleged crime scene, and rated the defendant's guilt on several items. Depending on condition, the photograph contained either RMA-applicable stimuli (e.g., alcoholic beverages) or neutral stimuli. Higher RMA predicted lower ratings of defendant guilt especially when applicable stimuli were present and RMA was strong. Study 2 (N = 85) showed that RMA-related attitude strength also moderated the effect of RMA on self-reported rape proclivity. Results of both studies indicate that the subjective strength of rape-related beliefs may be reliably assessed and serves as an important moderator of effects of RMA. The implications of these findings for prevention programs as well as future directions for research are discussed. PMID:23400886

  11. Attitudes toward rape: a comparison between Asian and Caucasian college students.

    PubMed

    Lee, Joohee; Pomeroy, Elizabeth C; Yoo, Seo-Koo; Rheinboldt, Kurt T

    2005-02-01

    The purpose of this study is to investigate differences in attitudes toward rape between Asian and Caucasian college students. The Attitudes Toward Rape scale was used to measure beliefs about rape in a convenience sample of 169 college students. Three items regarding stranger rape myths were added. Findings suggest that Asian students are more likely than Caucasian students to believe women should be held responsible for preventing rape and to view sex as the primary motivation for rape. Asians also have stronger beliefs than Caucasians do that victims cause the rape and that most rapists are strangers. This research suggests that outreach programs can play an important role in providing information, education, and prevention regarding rape and that males and Asian students should be target populations for such programs. PMID:16043546

  12. Perceptions of rape and attitudes toward women in a sample of Lebanese students.

    PubMed

    Rebeiz, Maria J; Harb, Charles

    2010-04-01

    This study investigated values, ambivalent sexism, religiosity, religious differences, gender, and attitudes toward rape victims as predictors of rape myths in a sample of Lebanese students (N = 300). Values of self-transcendence and conservation, gender, hostile sexism, and attitudes toward rape victims emerged as significant predictors of rape myths, confirming some of the premises in the literature. Type of rape (date, marital, acquaintance, and stranger rape) and victim's characteristics (widowed, married, devout, promiscuous, and chaste) were also investigated. Results revealed that no matter what the relationship between the victim and her perpetrator was, and regardless of the victim's characteristics, forcible sexual contact was always considered as rape. However, differences did emerge as to the degree to which these variations were perceived as rape. Implication of findings for research and the Lebanese culture are discussed. PMID:19494244

  13. POLY(LACTIC ACID) GREEN COMPOSITES USING OILSEED COPRODUCTS AS FILLERS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Poly(lactic acid), PLA, is a biodegradable polymer made from renewable resources with similar mechanical properties to polypropylene. PLA is more expensive than petroleum-based plastics, and the use of low-cost fillers as extenders is desirable. Agricultural co-products of the alternative oilseed ...

  14. GREEN COMPOSITES OF POLY(LACTIC ACID) AND OILSEED CO-PRODUCTS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Poly(lactic acid)(PLA) is a biodegradable plastic made from renewable resources and has similar mechanical properties to polypropylene. PLA is more expensive than petroleum-based plastics, and the use of low-cost fillers as extenders is desirable. Agricultural co-products (AcP) of oilseed crops we...

  15. Differential impacts of brassicaceous and nonbrassicaceous oilseed meals on soil bacterial and fungal communities.

    PubMed

    Hollister, Emily B; Hu, Ping; Wang, Autumn S; Hons, Frank M; Gentry, Terry J

    2013-03-01

    Demand for alternative fuels has sparked renewed interest in the production of biodiesel from oil-rich seeds. Oilseed meals are a byproduct of this process, and given their relatively high nutrient content, land application represents a potential value-added use. In this microcosm-based study, soil microbial community responses to amendments of a glucosinolate-containing brassicaceous oilseed meal (Brassica juncea, mustard), a non-glucosinolate-containing, nonbrassicaceous oilseed meal (Linum usitatissimum, flax), and a nonoilseed biomass (Sorghum bicolor) were characterized using a 28-day time series of replicated 16S rRNA gene and fungal ITS gene sequence libraries. We hypothesized that biomass type and glucosinolate content would alter community composition but that effects would diminish over time. Distinct separation occurred by amendment type, with mustard inducing large increases in the abundance of bacterial taxa associated with fungal disease suppression (e.g. Bacillus, Pseudomonas, and Streptomyces spp.). Dramatic shifts were seen among the fungi, too, with phylotype richness decreasing by > 60% following mustard addition. Changes in bacterial and fungal community composition were rapid, and distinct community types persisted throughout the study. Oilseed amendment, and mustard in particular, has the potential to alter soil microbial community structure substantially, and such changes are likely to be important in the context of ecosystem health. PMID:23025785

  16. 40 CFR 63.2855 - How do I determine the quantity of oilseed processed?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 12 2010-07-01 2010-07-01 true How do I determine the quantity of oilseed processed? 63.2855 Section 63.2855 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards...

  17. Material and interaction properties of selected grains and oilseeds for modeling discrete particles

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Experimental investigations of grain flow can be expensive and time-consuming, but computer simulations can reduce the large effort required to evaluate the flow of grain in handling operations. Published data on material and interaction properties of selected grains and oilseeds relevant to Discret...

  18. Reproductive allocation and nutrient relationships in Cuphea: A semi-domesticated oilseed crop

    Technology Transfer Automated Retrieval System (TEKTRAN)

    No information is available on allometric variations in C, N, and P in structural, reproductive and metabolic tissues and their ratios in Cuphea germplasm line PSR23, a semi-domesticated indeterminate and phenotypically plastic oilseed crop. The objectives of this study were to quantify the impact o...

  19. Registration of an oilseed sunflower germplasm HA-DM1 resistant to sunflower downy mildew

    Technology Transfer Automated Retrieval System (TEKTRAN)

    HA-DM1 (Reg. No.xxx, PI 674793) sunflower (Helianthus annuus L.) germplasm was developed and released cooperatively by the USDA-ARS, Sunflower and Plant Biology Research Unit and the North Dakota Agricultural Experiment Station in 2015. HA-DM1 is a BC2F4 derived oilseed maintainer line from the cros...

  20. Adaptability of cuphea, a new oilseed crop, to climate and soil environments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cuphea, a new oilseed crop rich in medium-chain fatty acids (C8:0 to C14:0), may serve as a renewable, biodegradable source of oil for lubricants, motor oil, and aircraft fuel. Impacts of climate and soil environment on cuphea growth and development are not well understood. The objective of this stu...

  1. Oilseed Radish (Raphanus Sativus) Effects on Soil Structure and Soil Water Relations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Oilseed radish (Raphanus sativus spp. oleifera) reduces nematode populations. Fall-incorporated radish biomass may also improve soil physical and hydraulic properties to increase the yield and quality of subsequently grown sugarbeet (Beta vulgaris L.). This field study determined radish effects on...

  2. Short communication: effect of oilseed supplementation of an herbage diet on ruminal fermentation in continuous culture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A four-unit continuous culture fermentor system was used to evaluate the effects of oilseed supplementation of an herbage-based diet on ruminal fermentation. Treatments were randomly assigned to fermentors in a 4 x 4 Latin square design with 7 d for diet adaptation and 3 d for data and sample collec...

  3. 40 CFR 63.2855 - How do I determine the quantity of oilseed processed?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 13 2014-07-01 2014-07-01 false How do I determine the quantity of oilseed processed? 63.2855 Section 63.2855 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National...

  4. EVALUATION OF BIODIESELS FROM SEVERAL OILSEED SOURCES AS ENVIRONMENTALLY-FRIENDLY CONTACT HERBICIDES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Postemergence contact herbicidal activities of biodiesels from several oilseed sources were examined for potential use to control broadleaf weeds in turfgrasses. Cuphea, lesquerella, meadowfoam, milkweed, and soybean oils were applied as 1 and 2 % (v/v) aqueous emulsions plus a nonionic surfactant ...

  5. 7 CFR 1412.35 - Incorrect or false production evidence of oilseeds and pulse crops.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Incorrect or false production evidence of oilseeds and pulse crops. 1412.35 Section 1412.35 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS DIRECT AND COUNTER-CYCLICAL PROGRAM AND...

  6. Life-Cycle Assessment of Oilseeds for Biojet Production Using Localized Cold-Press Extraction.

    PubMed

    Sieverding, Heidi L; Zhao, Xianhui; Wei, Lin; Stone, James J

    2016-05-01

    As nonfood oilseed varieties are being rapidly developed, new varieties may affect agricultural production efficiency and life-cycle assessment results. Current, detailed feedstock production information is necessary to accurately assess impacts of the biofuel life-cycle. The life-cycle impacts of four nonfood oilseeds (carinata [ L. Braun], camelina [ L. Crantz], canola or rapeseed [ L.], and sunflower [ L.]) were modeled using Argonne National Laboratory's GREET model to compare feedstocks for renewable biojet production using cold-press oil extraction. Only feedstock-related inputs were varied, allowing isolation of feedstock influence. Carinata and camelina performed slightly better than other oilseed crops at most product stages and impact categories as a result of current, low-input agricultural information and new feedstock varieties. Between 40 to 50% of SO and NO emissions, ∼25% of greenhouse gas (GHG) emissions, and ∼40% of total energy consumption for the biojet production impact occurred during feedstock production. Within the first standard deviation, total well-to-tank emissions varied between ∼13% (GHG) and ∼35% (SO) for all feedstocks emphasizing the importance of accurate agricultural production information. Nonfood oilseed feedstock properties (e.g., oil content, density) and agricultural management (e.g., fertilization, yield) affect life-cycle assessment results. Using biofuels in feedstock production and focusing on low-impact management would assist producers in improving overall product sustainability. PMID:27136164

  7. New oilseed crops for fuels and chemicals: ecological and agricultural considerations

    SciTech Connect

    Draper, H.M. III

    1982-01-01

    A new approach to agriculture involving oilseed crops for fuels and chemicals is proposed. Such an approach to biomass energy would be designed to benefit the limited-resource farmer in the United States and the Third World, while at the same time not aggravating global ecological problems such as deforestation and desertification. Since food versus fuel conflicts arise when plants are grown for industrial uses on good lands, productivity questions are examined, with the conclusion that fundamental biological constraints will limit yields on marginal lands. Conventional vegetable oil crops are limited in their climatic requirements or are not well adapted to limited-resource farming; therefore, new oilseeds more adaptable to small farming are proposed. Such plants would be for specialty chemicals or to meet local energy needs. Chemicals produced would be low-volume, labor-intensive, and possibly high-priced. A list of 281 potential new oilseeds is provided, and each is classified according to potential, multiple product potential, and vegetative characteristics. Using climatic data which are available for most areas, a method of making rough productivity estimates for unconventional wild plant oilseeds is proposed, and example resource estimates are provided for the southeastern United States.

  8. 40 CFR 63.2855 - How do I determine the quantity of oilseed processed?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 13 2013-07-01 2012-07-01 true How do I determine the quantity of oilseed processed? 63.2855 Section 63.2855 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National...

  9. Feasibility of cuphea as a new oilseed crop to climate and soil environments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cuphea, a new oilseed crop rich in medium-chain fatty acids (C8:0 to C14:0), may serve as a renewable, biodegradable source of oil for lubricants, motor oil, and aircraft fuel. Impacts of climate and soil environment on cuphea growth and development are not well understood. The objective of this stu...

  10. Management of Rhizoctonia Damping-off of Brassica Oilseed Crops in the PNW

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rhizoctonia solani can cause pre and post-emergence damping off of Brassica oilseed species with adverse effects on stand establishment. In greenhouse experiments, we have examined resistance to two groups (AGs) of Rhizoctonia solani among various Brassica species and varieties. R. solani AG 2-1 is ...

  11. Sexual climate and reported rape: a state-level analysis.

    PubMed

    Jaffee, D; Straus, M A

    1987-04-01

    Data on the US states are used to study two aspects of sexual climate: sexually liberal attitudes and sex magazine readership, and their relationship to reported rape. The two sexual climate measures are correlated (r = 0.50), but each also seems to measure a different facet of sexual climate: tolerance for the sexual behavior of others in the case of the Sexual Liberalism Index, and perhaps sexuality per se in the case of sex magazine readership. States in the Northeast have the highest average sexual liberalism score, and states in the West have the highest average readership of sex magazines. The relationship between sexual climate and the incidence of reported rape was investigated using multiple regression. Results indicate no relationship between sexually liberal attitudes and rape, but that sex magazine readership, urbanization, poverty, and a high percentage of divorced men are each significantly associated with the incidence of reported rape. The findings are interpreted within a theoretical model that posits rape as a function of social disorganization and hypermasculine gender roles and sexuality. PMID:3592959

  12. Suspect aggression and victim resistance in multiple perpetrator rapes.

    PubMed

    Woodhams, Jessica; Cooke, Claire

    2013-11-01

    Several research studies have reported an elevated level of aggression in rapes committed by multiple perpetrators compared to rapes committed by lone suspects. Several factors that have been linked to elevated aggression in generic samples of rape were examined for the first time with a sample of multiple perpetrator rapes. Factors that might be associated with victim resistance were also investigated. Victim and offender characteristics, as well as the behaviors displayed by victims and offenders, were extracted from the police files of 89 multiple perpetrator stranger rapes perpetrated against female victims in the United Kingdom. These behaviors were rated for their level of suspect (non-sexual) aggression and victim resistance, respectively. Degree of victim resistance was significantly and positively associated with suspect aggression. Older victims were the recipients of significantly higher levels of suspect aggression. Victims who were incapacitated from drugs and/or alcohol were less likely to be the recipients of suspect aggression. Group leaders displayed more aggression towards the victim than the followers in the groups. The number of perpetrators was significantly related to the degree of resistance displayed by the victim with offences perpetrated by fewer suspects being characterized by more victim resistance. Research regarding cognitive appraisal during criminal interactions and the respective roles of offenders is referred to in considering these relationships. PMID:23740469

  13. Effects of Victim Gaze Behavior and Prior Relationship on Rape Culpability Attributions.

    ERIC Educational Resources Information Center

    Willis, Cynthia E.; Wrightsman, Lawrence S.

    1995-01-01

    Rape victims' gaze behavior when identifying a defendant and the prior relationship between victim and defendant were examined for effects on rape culpability attributions. In comparison with victims who used gaze maintenance or natural gaze behavior, rape victims' gaze avoidance was perceived as indicative of less truthfulness rather than…

  14. The Long-Term Effects of Rape on Lifestyle and Psychological Functioning.

    ERIC Educational Resources Information Center

    Esper, Jody A.; Runge, Christopher J.

    Research has shown rape to be an act of violence which affects the lives of many individuals each year. For many survivors of rape, effects on psychological functioning and lifestyle may endure for many years. This study was undertaken to develop and pilot the Rape Assessment Schedule, an interview schedule designed to assess the very long-term…

  15. Changing the Rape-Supportive Attitudes of Traditional and Nontraditional Male and Female College Students.

    ERIC Educational Resources Information Center

    Rosenthal, Eric H.; And Others

    1995-01-01

    Tested whether individuals possessing traditional sex role attitudes could have their rape-related attitudes influenced by a psychoeducational intervention that had been effective with less traditional individuals. Results revealed that subjects who received intervention adhered less to rape myths and expressed less rape-supportive attitudes than…

  16. Women's Attitudes and Fantasies about Rape as a Function of Early Exposure to Pornography.

    ERIC Educational Resources Information Center

    Corne, Shawn; And Others

    1992-01-01

    Tested hypothesis that women's attitudes and fantasies about rape arise partially from their socialization to accept sexual aggression as normative. Female participants answered questions on childhood exposure to pornography, sex fantasies, and rape attitudes. Early exposure to pornography correlated to "rape fantasies" and attitudes supportive of…

  17. Rape Prevention Education for Men: The Effectiveness of Empathy-Induction Techniques.

    ERIC Educational Resources Information Center

    Berg, Dianne R.; Lonsway, Kimberly A.; Fitzgerald, Louise F.

    1999-01-01

    Fifty-four male undergraduates participated in a rape prevention education program in which the experimental group listened to an audiotape of a man versus a woman describing the experience of being raped. Two weeks later, the students who heard the female tape reported more likelihood to engage in rape-supportive behaviors but no difference in…

  18. A Comparison of Strategies for Changing College Students' Attitudes toward Acquaintance Rape.

    ERIC Educational Resources Information Center

    Nelson, Eileen S.; Torgler, Cyndee C.

    1990-01-01

    Compared effects of strategies for informing college students about date rape. Assigned students to either experimental group that viewed videotape on acquaintance rape (n=33), experimental group that read brochure on acquaintance rape (n=31), or control group (n=25). Findings revealed no significant difference between effects of videotape and…

  19. The Impact of Acquaintance Rape Stories and Case-Specific Pretrial Publicity on Juror Decision Making.

    ERIC Educational Resources Information Center

    Mullin, Charles; And Others

    1996-01-01

    Reports on a trial simulation in which exposure to publicity regarding the defendant and general stories about acquaintance rape were manipulated; participants then viewed an enacted rape trial. Finds that male subjects exposed to predatory rape publicity were pro-defendant, whereas women were unaffected. Discusses male reaction to information…

  20. Date Violence and Date Rape among Adolescents: Associations with Disordered Eating Behaviors and Psychological Health.

    ERIC Educational Resources Information Center

    Ackard, Diann M.; Neumark-Sztainer, Dianne

    2002-01-01

    A study assessed prevalence of date violence and rape in 81,247 adolescents. Approximately 9% of girls and 6% of boys had experienced date violence or rape. Date violence and rape was associated with higher rates of disordered eating behaviors and suicidal thoughts and attempts, and lower emotional well-being and self-esteem. (Contains…

  1. 73 FR 9142 - Hearing of the Review Panel on Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2008-02-19

    ... Office of Justice Programs Hearing of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... third hearings of the Review Panel on Prison Rape (Panel), which will be held in Washington, DC, on... common characteristics of victims and perpetrators of prison rape, and prison systems with the...

  2. 73 FR 65404 - Hearing of the Review Panel on Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2008-11-03

    ... of Justice Programs Hearing of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Review Panel on Prison Rape (Panel) will hold a hearing in Culpeper, Virginia on November 14, 2008. The... Statistics (BJS) in identifying common characteristics of victims and perpetrators of rape in county...

  3. 79 FR 48252 - Hearings of the Review Panel on Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2014-08-15

    ... of Justice Programs Hearings of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Review Panel on Prison Rape (Panel) will hold hearings in Washington, DC, on August 28, 2014. The hearing... INFORMATION: The Panel, which was established pursuant to the Prison Rape Elimination Act of 2003, Public...

  4. 74 FR 63792 - Solicitation for a Cooperative Agreement-The Prison Rape Elimination Act (PREA) Technical...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2009-12-04

    ... National Institute of Corrections Solicitation for a Cooperative Agreement--The Prison Rape Elimination Act... 12-month period to begin in February 2010. Section 5 of the Prison Rape Elimination Act (PREA... responsible for the prevention, investigation, and punishment of instances of prison rape.'' NIC's...

  5. 71 FR 62488 - Hearing of the Review Panel on Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2006-10-25

    ... of Justice Programs Hearing of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... hearing of the Review Panel on Prison Rape (Panel), which will be held in Represa, California, on November... identify common characteristics, not only of victims and perpetrators of prison rape, but also of...

  6. 73 FR 54856 - Hearing of the Review Panel on Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2008-09-23

    ... of Justice Programs Hearing of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Panel on Prison Rape (Panel), will hold hearings in Springfield, Massachusetts, on September 24, 2008... common characteristics of victims and perpetrators of prison rape, and prison and prison systems with...

  7. 73 FR 48394 - Hearing of the Review Panel on Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2008-08-19

    ... of Justice Programs Hearing of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Review Panel on Prison Rape (Panel), will hold hearings in Orlando, Florida on August 27, 2008... characteristics of victims and perpetrators of prison rape, and prison and prison systems with the highest...

  8. 70 FR 13033 - Building Comprehensive Prevention Program Planning and Evaluation Capacity for Rape Prevention...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2005-03-17

    ... Planning and Evaluation Capacity for Rape Prevention and Education Funded Programs Announcement Type: New... evaluation capacity among selected Rape Prevention and Education (RPE) funded sexual violence prevention... program planning and evaluation will be focused on the national Rape Prevention and Education...

  9. An acute post-rape intervention to prevent substance use and abuse.

    PubMed

    Acierno, Ron; Resnick, Heidi S; Flood, Amanda; Holmes, Melisa

    2003-12-01

    The trauma of rape is routinely associated with extreme acute distress. Such peri-event anxiety increases risk of developing psychopathology and substance use or abuse post-rape, with the degree of initial distress positively predicting future problems. Unfortunately, the nature of post-rape forensic evidence collection procedures may exacerbate initial distress, thereby potentiating post-rape negative emotional sequelae. Consequently, substance use may increase in an effort to ameliorate this distress. To address this, a two-part video intervention was developed for use in acute post-rape time frames to (a) minimize anxiety during forensic rape examinations, thereby reducing risk of future emotional problems, and (b) prevent increased post-rape substance use and abuse. Pilot study data with 124 rape victims indicated that the low-cost, easily administered intervention was effective in reducing risk of marijuana abuse at 6 weeks. Nonstatistically significant trends also were evident for reduced marijuana use. Trends were also noted in favor of the intervention in the subgroup of women who were actively using substances pre-rape (among pre-rape alcohol users, 28% viewers vs. 43% nonviewers met criteria for post-rape alcohol abuse; among pre-rape marijuana users, the rates of post-marijuana use were 17% vs. 43%). PMID:14656554

  10. Peer Educators and Close Friends as Predictors of Male College Students' Willingness to Prevent Rape

    ERIC Educational Resources Information Center

    Stein, Jerrold L.

    2007-01-01

    Astin's (1977, 1991, 1993) input-environment-outcome (I-E-O) model provided a conceptual framework for this study which measured 156 male college students' willingness to prevent rape (outcome variable). Predictor variables included personal attitudes (input variable), perceptions of close friends' attitudes toward rape and rape prevention…

  11. Rape Perception and the Function of Ambivalent Sexism and Gender-Role Traditionality

    ERIC Educational Resources Information Center

    Yamawaki, Niwako

    2007-01-01

    This study explores the roles of benevolent sexism (BS), hostile sexism (HS), and gender-role traditionality (GRT) in minimizing rape, blaming the victim, and excusing the rapist. As predicted, hostile sexists minimize the seriousness of the rape in both stranger and date-rape scenarios. In the victim-blame scale, both BS and GRT significantly…

  12. From Survivor to Thriver: A Pilot Study of an Online Program for Rape Victims

    ERIC Educational Resources Information Center

    Littleton, Heather; Buck, Katherine; Rosman, Lindsey; Grills-Taquechel, Amie

    2012-01-01

    Approximately 15% to 20% of women have been victims of rape and close to a third report current rape-related PTSD or clinically significant depression or anxiety. Unfortunately, very few distressed rape victims seek formal help. This suggests a need to develop alternative ways to assist the many distressed victims of sexual violence. Online…

  13. Understanding Rape Survivors' Decisions Not to Seek Help from Formal Social Systems

    ERIC Educational Resources Information Center

    Patterson, Debra; Greeson, Megan; Campbell, Rebecca

    2009-01-01

    Few rape survivors seek help from formal social systems after their assault. The purpose of this study was to examine factors that prevent survivors from seeking help from the legal, medical, and mental health systems and rape crisis centers. In this study, 29 female rape survivors who did not seek any postassault formal help were interviewed…

  14. 75 FR 11077 - National Standards To Prevent, Detect, and Respond to Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-10

    ... Part 115 RIN 1105-AB34 National Standards To Prevent, Detect, and Respond to Prison Rape AGENCY... abuse in confinement settings that were prepared by the National Prison Rape Elimination Commission (Commission) pursuant to the Prison Rape Elimination Act of 2003 (PREA) and recommended by the Commission...

  15. Understanding Rape and Sexual Assault: 20 Years of Progress and Future Directions

    ERIC Educational Resources Information Center

    Campbell, Rebecca; Wasco, Sharon M.

    2005-01-01

    During the past 20 years, researchers have documented the widespread problem of rape in American society. Approximately one in four women are raped in their adult lifetime, which causes severe psychological distress and long-term physical health problems. The impact of sexual assault extends far beyond rape survivors as their family, friends, and…

  16. A Socioecological Model of Rape Survivors' Decisions to Aid in Case Prosecution

    ERIC Educational Resources Information Center

    Anders, Mary C.; Christopher, F. Scott

    2011-01-01

    The purpose of our study was to identify factors underlying rape survivors' post-assault prosecution decisions by testing a decision model that included the complex relations between the multiple social ecological systems within which rape survivors are embedded. We coded 440 police rape cases for characteristics of the assault and characteristics…

  17. 75 FR 27000 - Hearings of the Review Panel on Prison Rape

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-13

    ... of Justice Programs Hearings of the Review Panel on Prison Rape AGENCY: Office of Justice Programs... Review Panel on Prison Rape (Panel) will hold hearings in Washington, DC on June 3-4, 2010. The hearing... incidence of rape, respectively, based on an anonymous survey by the BJS of youth in a representative...

  18. Current Perceptions of Marital Rape: Some Good and Not-So-Good News

    ERIC Educational Resources Information Center

    Ferro, Christine; Cermele, Jill; Saltzman, Ann

    2008-01-01

    The current study extends previous research on marital rape and acceptance of general rape myths by comparing the perceptions of undergraduate college students (n = 85) to those of college alumni/ae (n = 44) who graduated from the same university three decades earlier. Participants read a hypothetical rape scenario that depicted the perpetrator as…

  19. Perceptions of Rape and Attitudes toward Women in a Sample of Lebanese Students

    ERIC Educational Resources Information Center

    Rebeiz, Maria J.; Harb, Charles

    2010-01-01

    This study investigated values, ambivalent sexism, religiosity, religious differences, gender, and attitudes toward rape victims as predictors of rape myths in a sample of Lebanese students (N = 300). Values of self-transcendence and conservation, gender, hostile sexism, and attitudes toward rape victims emerged as significant predictors of rape…

  20. The Impact of Past Sexual Experiences on Attributions of Responsibility for Rape

    ERIC Educational Resources Information Center

    Mason, Gillian E.; Riger, Stephanie; Foley, Linda A.

    2004-01-01

    Two factors potentially affect observers' attributions of responsibility to a rape survivor: how closely they identify with the survivor and how much they adhere to rape myths. To assess the impact of these factors, 157 female college students categorized by their sexual assault history and by their acceptance of rape myths, evaluated a sexual…