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Possible Tyromyces fissilis (Basidiomycota, Polyporales) co-infection in a lung transplant recipient.  


Invasive fungal diseases are severe complication of the lung transplant patients' follow-up as they are increasing the risk of rejection. We report a patient who developed possible Tyromyces fissilis co-infection during graft rejection episode 2 years after bilateral lung transplantation for cystic fibrosis. The fungus was detected using conventional culture methods as a filamentous basidiomycete and further placed to T. fissilis species based on internal transcribed spacer (ITS) rDNA sequences. The patient was treated according to the susceptibility testing results by voriconazole in combination with the anti-rejection therapy and recovered completely within few weeks. This is, to our knowledge, the first published case report of T. fissilis as a possible causative agent of an infection/rejection episode in a lung transplant recipient. PMID:25064474

Chrenkova, Vanda; Kolarik, Miroslav; Hubacek, Petr; Kolarik, Jan; Simonek, Jan; Lischke, Robert; Drevinek, Pavel



Effect of latitudinal gradient and impact of logging on genetic diversity of Cedrela lilloi along the Argentine Yungas Rainforest  

PubMed Central

Cedrela lilloi C. DC. (cedro coya, Meliaceae), an important south American timber species, has been historically overexploited through selective logging in Argentine Yungas Rainforest. Management and conservation programs of the species require knowledge of its genetic variation patterns; however, no information is available. Molecular genetic variability of the species was characterized to identify high-priority populations for conservation and domestication purposes. Fourteen native populations (160 individuals) along a latitudinal gradient and with different logging's intensities were assessed by 293 polymorphic AFLP (amplified fragment length polymorphism) markers. Genetic diversity was low (Ht = 0.135), according to marginal location of the species in Argentina. Most of the diversity was distributed within populations (87%). Northern populations showed significant higher genetic diversity (R2= 0.69) that agreed with latitudinal pattern of distribution of taxonomic diversity in the Yungas. Three clusters were identified by Bayesian analysis in correspondence with northern, central, and southern Yungas. An analysis of molecular variance (AMOVA) revealed significant genetic differences among latitudinal clusters even when logging (?RT = 0.07) and unlogging populations (?PT = 0.10) were separately analyzed. Loss of genetic diversity with increasing logging intensity was observed between neighboring populations with different disturbance (?PT = 0.03–0.10). Bottlenecks in disturbed populations are suggested as the main cause. Our results emphasize both: the necessity of maintaining the genetic diversity in protected areas that appear as possible long-term refuges of the species; and to rescue for the national system of protected areas some high genetic diversity populations that are on private fields. PMID:23170208

Inza, Maria V; Zelener, Noga; Fornes, Luis; Gallo, Leonardo A



Conserving the Pines of Guadalupe and Cedros Islands, Mexico: An International Collaboration  

Microsoft Academic Search

Monterey pine (Pinus radiata D. Don) is an enigmatic species. Native to only Mexico and USA, it is restricted to three populations along the central coast of California and one on each of two Mexican islands off Baja California— Guadalupe and Cedros Islands. Commercially, it is grown in exotic plantations worldwide on over 4 million hectares with high economic value,

Deborah L. Rogers; J. Jesús Vargas Hernández; A. Colin Matheson; Jesús J. Guerra Santos


Threat to Cedar, Cedrela odorata, Plantations in Vietnam by the Weevil, Aclees sp.  

PubMed Central

The recent decline and death of young cedar, Cedrela odorata L. (Sapindales: Meliaceae), plantations in Vietnam is caused by Aclees sp. (Coleoptera: Curculionidae), a wood-boring brown weevil. A field study was undertaken in three-year-old plantations in two districts in Thanh Hoa province in August 2008. Trees were heavily impacted by the weevil, Aclees; the infestation level (P) ranged from 80 to 100% and the average damage index (R) ranged from 1.8 to 2.8. Observations over one year enabled the life history to be determined. Eggs were laid (February to March, September to November) inside the bark from the base of the trunk up to 60 cm in height. Larvae formed extensive feeding tunnels in the inner bark and sap wood. Pupation occurred in feeding tunnels or pupal chambers in the sapwood. Adults emerged twice a year, February to March and August to October. It is concluded that Aclees is a threat to C. odorata plantations in tropical regions of the world, and quarantine measures should be implemented to reduce the risk of spread. PMID:21271846

Thu, Pham Quang; Quang, Dao Ngoc; Dell, Bernard



Effects of Light Intensity and Nutrient on Growth and Electron Transport Rate of Tropical Tree Seedlings (Bombax buonopozense, Khaya ivorensis and Cedrela odorata) found in Ghana  

Microsoft Academic Search

physiological process. Actual levels of light and nutrient requirements for Bombax buonopozense and Khaya ivorensis tree species native of Ghana and Cedrela odorata (exotic) are not known. This study therefore attempted to find out the level of nutrient and light requirements as well as their interactive effect on growth and electron transport rate (a measure of photosynthetic process) of the

Richard GYIMAH; Toshio NAKAO


Long-term trends in cellulose delta13 C and water-use efficiency of tropical Cedrela and Swietenia from Brazil.  


Elevated CO(2) concentrations ([CO(2)]) affect plant water relations and photosynthesis, and the increase in atmospheric [CO(2)] over the past 100-200 years has been related to changes in stomatal density and the carbon isotope ratio (delta(13)C) in tree rings and leaves from herbarium specimens. Because many tropical trees do not produce annual growth rings and their wood is therefore difficult to date, no trends in delta(13)C of tropical trees have been reported. Wood from Cedrela odorata L. (tropical cedar) and Swietenia macrophylla King (bigleaf mahogany), which do produce annual rings, was collected from a primary rain forest in Aripuanã, Brazil (10 degrees 09' S, 59 degrees 26' W). We measured wood cellulose delta(13)C in 10-year growth increments from 37 Cedrela trees (between 11 and 151 years old in 2001) and 16 Swietenia trees (48-126 years old). A comparison of delta(13)C in cellulose of trees from different decades and of trees of different cambial ages showed that the amount of delta(13)C was largely related to the decade the wood was produced in, and not, or only to a minor extent, to tree age. Cellulose delta(13)C decreased from -26.0 to -27.3 per thousand in Cedrela and from -25.7 to -27.1 per thousand in Swietenia, with the largest changes occurring during the past 50 years. Based on these data and the trends in atmospheric [CO(2)] and delta(13)CO(2), we calculated that the internal [CO(2)] increased from about 220 to 260 ppm and that intrinsic water-use efficiency increased by 34% in Cedrela and by 52% in Swietenia. This may have implications for the water cycle and may explain the trend toward increased tree growth and turnover observed in some tropical forests. PMID:15805094

Hietz, Peter; Wanek, Wolfgang; Dünisch, Oliver



75 FR 47301 - Cedro Hill Wind LLC; Butler Ridge Wind Energy Center, LLC; High Majestic Wind Energy Center, LLC...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Loraine Windpark Project, LLC; White Oak Energy LLC; Meadow Lake Wind Farm III LLC; Meadow Lake Wind Farm IV LLC; Blackstone Wind Farm II LLC; Otay Acquisition Company, LLC; TX Solar I LLC; CalRENEW-1 LLC; Notice of Effectiveness of...



Identification of endangered or threatened Costa Rican tree species by wood anatomy and fluorescence activity.  


A total of 45 native Costa Rican tree species are threatened or in danger of extinction, but the Convention on International Trade Endangered Species (CITES) includes only eight of these in its Appendices. However, the identification of other species based on their wood anatomy is limited. The present study objective was to describe and to compare wood anatomy and fluorescence activity in some endangered or threatened species of Costa Rica. A total of 45 (22 endangered and 23 threatened with extinction) wood samples of these species, from the xylaria of the Instituto Tecnológico de Costa Rica and the Forest Products Laboratory in Madison, Wisconsin, were examined. Surface fluorescence was positive in eight species, water extract fluorescence was positive in six species and ethanol extract fluorescence was positive in 24 species. Almost all species were diffuse porous except for occasional (Cedrela odorata, C. fissilis, Cordia gerascanthus) or regular (C. salvadorensis and C. tonduzii) semi-ring porosity. A dendritic vessel arrangement was found in Sideroxylon capari, and pores were solitary in Guaiacum sanctum and Vantanea barbourii. Vessel element length was shortest in Guaiacum sanctum and longest in Humiriastrum guianensis, Minquartia guianensis and Vantanea barbourii. Finally, anatomical information and fluorescence activity were utilized to construct an identification key of species, in which fluorescence is a feature used in identification. PMID:24027913

Moya, Róger; Wiemann, Michael C; Olivares, Carlos



The Lepidoptera associated with forestry crop species in Brazil: a historical approach.  


Despite the long history of forestry activity in Brazil and its importance to the national economy, there is still much disorder in the information regarding pests of forestry species. Considering the importance of the entomological knowledge for the viability of silvicultural management, this work aimed to gather information on the species of Lepidoptera associated with forestry crops within Brazil using a historical approach. Through a literature review, all registered species of Lepidoptera related to forestry crops in Brazil from 1896 to 2010 were identified. The historical evaluation was based on the comparison of the number of published articles, species richness, and community similarities of the Lepidoptera and their associated forest crops, grouped in 10-year samples. A total of 417 occurrences of Lepidoptera associated with forestry species were recorded, from which 84 species are related with 40 different forestry crops. The nocturnal Lepidoptera were dominant on the records, with Eacles imperialis magnifica Walker as the most frequent pest species cited. Myrtaceae was the most frequent plant family, with Cedrela fissilis as the most cited forestry crop species. A successional change in both Lepidoptera species and their host plants was observed over the decades. The richness of lepidopteran pest species increased over the years, unlike the richness of forestry crop species. This increase could be related to the inefficient enforcement of sanitary barriers, to the increase of monoculture areas, and to the adaptability of native pests to exotic forestry species used in monoculture stands. PMID:23950084

Kowalczuck, Manoela; Carneiro, E; Casagrande, M M; Mielke, O H H



Endosymbiotic copepods may feed on zooxanthellae from their coral host, Pocillopora damicornis  

NASA Astrophysics Data System (ADS)

The Xarifiidae is one of the most common families of endosymbiotic copepods that live in close association with scleractinian corals. Previous studies on xarifiids primarily focused on their taxonomy and morphology, while their influence on corals is still unknown. In this study, we collected a total of 1,579 individuals belonging to 6 species of xarifiids from 360 colonies of Pocillopora damicornis at Nanwan Bay, southern Taiwan from July 2007 to May 2008. Furthermore, using optical and electron microscopic observations, we examined the gut contents of Xarifia fissilis, the most abundant species of the Xarifiidae that we collected. We found that the gut of X. fissilis was characterized by a reddish-brown color due to the presence of numerous unicellular algae with diameters of 5-10 ?m. TEM observations indicated that the unicellular algae possessed typical characteristics of Symbiodinium including a peripheral chloroplast, stalked pyrenoids, starch sheaths, mesokaryotic nuclei, amphiesmas, an accumulation body, and mitochondria. After starving the isolated X. fissilis in the light and dark (light intensity: 140 ?mol photon m-2 s-1; photoperiod: 12 h light/12 h dark) for 2 weeks, fluorescence was clearly visible in its gut and fecal pellets under fluorescent microscopic observations. The cultivation experiment supports the hypothesis that the unicellular algae were beneficial to the survival of X. fissilis under light conditions, possibly through transferring photosynthates to the hosts. These results suggest that X. fissilis may consume and retain unicellular algae for further photosynthesis.

Cheng, Y.-R.; Dai, C.-F.



Dendro Provenancing International meeting on historic  

E-print Network

Dendro ­ Provenancing International meeting on historic timber trade and dendrochronology Lithuania: Timber in the East Alar Läänelaid Dendrochronological dating of pieces of art in Estonia made of Baltic Koval Dendrochronology in Ukraine Tomasz Wany Survey of oak chronologies in Poland Anna Cedro


A new species of Jahnulales from Las Ilusiones Lagoon, Tabasco, Mexico  

Technology Transfer Automated Retrieval System (TEKTRAN)

During a study on biodiversity of freshwater ascomycetes from an urban tropical lagoon, an ascomycete with similar morphology to species of Jahnulales was obtained. Smooth surface test blocks of Pinus sp., Bucida sp., Cedrela sp. and Tabebuia sp. were submerged in pairs close to a private house whar...



E-print Network

exemples de Jersey, de Saint-Pierre-et-Miquelon et de Trinidad JURY M. Pascal BUL�ON (directeur), Directeur (Trinidad-et-Tobago), et Raphael SEBASTIAN, mon guide auprès des pêcheurs de Cedros. Je n'oublie pas-Pierre-et-Miquelon, archipel français d'Amérique du Nord Trinidad, île principale de la République de Trinidad

Paris-Sud XI, Université de


Evaluation of a Translocated Population of Desert Mule Deer in the Chihuahuan Desert of Northern Coahuila, Mexico  

E-print Network

, and northwestern Baja 3 California Norte; peninsula mule deer (O. h. peninsulae) occurring in Baja California Sur and Baja California Norte; Tiburon Island mule deer (O. h. sheldoni) inclusive to Tiburon Island; and Cedros Island mule deer (O. h. cerrosensis... mule deer in Mexico generally describe a distribution that includes the states of Coahuila, Chihuahua, Sonora, and Baja California Norte and portions of northeastern Durango, northern Zacatecas, western Nuevo Leon, northern San Luis Potosi...

Ortega-Sanchez, Alfonso



Biological activity of two neem ( Azadirachta indica A. Juss., Meliaceae) products on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae  

Microsoft Academic Search

Biological activity of two neem products (Azatin and Nim 80) on mahogany shootborer (Hypsipyla grandella) larvae was studied. A randomized complete block design, with four replications, was used. H. grandella third instar larvae were exposed for 24h to Cedrela odorata leaf discs dipped in several treatment dissolutions of each formulated product (0.1, 0.32, 1.0, 3.20, and 10%); afterwards, each larva

Fernando Mancebo; Luko Hilje; Gerardo A Mora; Rodolfo Salazar



Allometric equations for four valuable tropical tree species  

Microsoft Academic Search

Four tree species were harvested periodically over a 13-year period from plantations in the humid lowlands of Costa Rica: Cedrela odorata, Cordia alliodora, Hyeronima alchorneoides, and Euterpe oleracea. The soil was a well-drained, volcanic alluvium, and high fertility coupled with 4m of annual rainfall and high temperatures led to rapid growth rates; at age 13 many individual were >30cm dbh

Thomas G. Cole; John J. Ewel



[Chemical and biological evaluation of the effect of plant extracts against Plasmodium berghei].  


Extracts from thirteen species of plants were evaluated by "in vivo" antimalarial test against plasmodium berghei effects. Significant activities were observed in the ethyl acetate and aqueous extracts, elaborated of Cedrela tonduzii leaves, Trichilia havanensis and Trichilia americana barks, Neurolaena lobata and Gliricidia sepium leaves and Duranta repens fruits. Compounds identified include flavanoids, coumarins, mellilotic acid and iridoids which some kind of biodynamic activity has previously been reported. The flavone quercetin 1 purified from C. tonduzii gave strong antimalarial activity, however, its respective glucosides (quercetin 3-glucoside 2 y robinine 7) showed little significant activity. PMID:9246360

Castro, O; Barrios, M; Chinchilla, M; Guerrero, O



A Re-examination of Shallow Paleomagnetic Inclinations From the Cretaceous Valle Group Sedimentary Rocks, Baja California, Mexico  

NASA Astrophysics Data System (ADS)

A paleomagnetic, rock magnetic, and sedimentological study was conducted in order to determine whether depositional/compactional processes have caused the shallow inclinations observed in the Valle Group sedimentary rocks. A total of 126 samples (14 sites) were collected from the middle Cenomanian section of the Valle along the northern coast of the Vizcaino Peninsula, Baja California, approximately 20 km east of Punta Eugenia at Campito. Samples were subjected to detailed thermal and alternating field (af) demagnetization, typically in 14 steps to 610° C for thermal demagnetization and ~24 steps to 130 mT for af demagnetization. NRMs were strong for marine sedimentary rocks, typically 10 mA/m. The mean of the site means for the demagnetized data was Inc=54.2° , Dec=306° , ? 95=4.8° , N=12, in geographic coordinates, and Inc=20.5° , Dec=341.3° , ? 95=4° , N=12 in stratigraphic coordinates. AMS fabrics have minimum axes clustered nearly perpendicular to bedding, typical of primary depositional/compactional fabrics. Some sites exhibited minimum axes clustering about 10° from the vertical and maximum axes clustered about 10° from the horizontal suggesting that currents and/or initial bedding dip affected the magnetization of these samples at deposition. Since the stratigraphy of the Valle Group dips consistently to the NE at approximately 50° , we sampled a tight slump fold at one site in order to constrain the age of magnetization. Both the AMS fabric and the characteristic remanence (ChRM) fail the fold test at the 95% confidence level. At another site, we sampled adjacent beds each approximately 5 cm thick composed of coarse, medium, or fine-grained sandstone. The directions of these beds are within 2° of each other. These results can be interpreted to indicate either a late remagnetization of the Valle group or an acquisition of the Valle's detrital remanence after slumping, but early in the rock's post-depositional history. Smith and Busby's (1993) slump block test from nearby Cedros Island supports an early acquisition of magnetization for the Valle. Also, a positive regional tilt test for the site means at Campito and Cedros Island suggests that magnetization acquisition predates the regional tilting. Anisotropy of remanence measurements will be conducted to correct any inclination shallowing due to compaction since our mean stratigraphic direction is about 15° shallower than the inclination observed by Smith and Busby for the Valle at Cedros Island. Tectonic implications of the corrected results will be discussed.

Li, Y.; Kodama, K. P.; Smith, D. P.



Modeling Long-term Creep Performance for Welded Nickel-base Superalloy Structures for Power Generation Systems  

SciTech Connect

We report here a constitutive model for predicting long-term creep strain evolution in ?’ strengthened Ni-base superalloys. Dislocation climb-bypassing ?’, typical in intermediate ?’ volume fraction (~20%) alloys, is considered as the primary deformation mechanism. Dislocation shearing ?’ to anti-phase boundary (APB) faults and diffusional creep are also considered for high-stress and high-temperature low-stress conditions, respectively. Additional damage mechanism is taken into account for rapid increase in tertiary creep strain. The model has been applied to Alloy 282, and calibrated in a temperature range of 1375-1450?F, and stress range of 15-45ksi. The model parameters and a MATLAB code are provided. This report is prepared by Monica Soare and Chen Shen at GE Global Research. Technical discussions with Dr. Vito Cedro are greatly appreciated. This work was supported by DOE program DE-FE0005859

Shen, Chen



[Lipid and fatty acid profile of Perna viridis, green mussel (Mollusca: Bivalvia) in different areas of the Eastern Venezuela and the West Coast of Trinidad].  


The species Perna viridis is a highly consumed species, which fast growth makes it an interesting aquaculture alternative for Venezuelan and Trinidad coasts. With the aim to contribute with its nutritional value information, this study analyzed lipid and fatty acid contents from samples taken in five locations from Eastern Venezuela and three from Trinidad West Coast. Total lipids were extracted and quantified, from a pooled sample of 100 organisms per location, by standard gravimetric methods, and their identification and quantification was done by TLC/FID (Iatroscan system). Furthermore, the esterified fatty acids of total lipid, phospholipids and triacylglycerols were identified and quantified by gas chromatography. Eastern Venezuela samples from Los Cedros, La Brea and Chaguaramas showed the highest total lipid values of 7.92, 7.74 and 7.53, respectively, and the minimum values were obtained for La Restinga (6.08%). Among lipid composition, Chacopata samples showed the lowest phospholipid concentration (48.86%) and the maximum values for cholesterol (38.87%) and triacylglycerols (12.26%); besides, La Esmeralda and Rio Caribe samples exhibited maximum phospholipids (88.71 and 84.93 respectively) and minimum cholesterol (6.50 and 4.42%) concentrations. Saturated fatty acids represented between 15.04% and 65.55% within total lipid extracts, with maximum and minimum values for La Esmeralda and Chacopata, respectively. Polyunsaturated results resulted between 7.80 and 37.18%, with higher values in La Brea and lower values in La Esmeralda. For phospholipids, saturated fatty acids concentrations varied between 38.81 and 48.68% for Chaguaramas and Chacopata samples, respectively. In the case of polyunsaturated fatty acids, these varied between non detected and 34.51%, with high concentrations in Los Cedros (27.97%) and Chaguaramas (34.51%) samples. For the triacylglycerols, the saturated fatty acids composition oscillated between 14.27 and 53.80% with low concentrations for Chacopata and high concentration for La Restinga; the polyunsaturated fatty acids were between 4.66 and 35.55% with lower values for Chacopata and higher values for Chaguaramas samples. P. viridis is recommended for human being consumption, according to the high content of unsaturated fatty acids found for this species. PMID:21516644

Koftayan, Tamar; Milano, Jahiro; D'Armas, Haydelba; Salazar, Gabriel



Reinterpretation of Mesozoic ophiolite arc, and blueschist terranes in southwestern Baja California  

SciTech Connect

The nature and significance of disrupted Mesozoic oceanic rocks on Isla Santa Margarita and Isla Magdalena, western Baja California Sur, have been reinterpreted on the basis of detailed mapping and petrologic studies. Three structural units are recognized. (1) The upper plate consists of ophiolitic, arc, and forearc basin rocks. Ophiolitic rocks, including metamorphosed ultramafic rocks, gabbro, dikes, volcanic rocks, and chert, underwent strong contractional deformation and penetrative greenschist-facies metamorphism. Arc rocks, including gabbro, a dike and sill complex, compositionally diverse volcanic rocks, lahars, and volcaniclastic strata, lack a penetrative fabric and are weakly metamorphosed. Forearc basin rocks consist of unmetamorphosed conglomerated and rhythmically bedded siliciclastic turbidites. (2) The lower plate is a subduction complex consisting of weakly to moderately foliated and metamorphosed pillow and massive lavas, breccia, and tuff( ), interbedded red and green siliceous argillite, and rare radiolarian ribbon chert and limestone. Blueschist-facies metamorphism is indicated by lawsonite, aragonite, sodic amphibole, and sodic clino-pyroxene. (3) Serpentine-matrix melange crops out in shallowly dipping fault zones between the upper and lower plates. The structural and petrologic characteristics of the Mesozoic units, the geometry of contacts between them, and the age of extension are similar to those in the Isla Cedros-Vizcalno Peninsula region, 400 km to the northwest. The author infers that syn-subduction extension was a regional event that affected much of the western Baja forearc during the Late Cretaceous and Paleogene.

Sedlock, R.L. (San Jose State Univ., CA (United States). Geology Dept.)



The influence of mesoscale physical structures in the phytoplankton taxonomic composition of the subsurface chlorophyll maximum off western Baja California  

NASA Astrophysics Data System (ADS)

The distribution of the subsurface chlorophyll maximum (SCM) layer, its taxonomic phytoplankton composition, and the maximum quantum efficiency of charge separation of PSII (Fv/Fm) was investigated in the west coast off Baja California during October 2003. SCM characteristics were described and related to the hydrographic regime and the mesoscale physical structures present during this period. Seven groups of phytoplankton were detected in the SCM based on chemotaxonomic analysis of pigment fingerprints: diatoms, haptophytes, pelagophytes, prasinophytes, cryptophytes, Prochlorococcus and cyanobacteria. The distribution of these groups was heterogeneous and closely related to the circulation patterns characterized by the interaction of subarctic and tropical water. Eddies and meanders were detected in the study area and these structures exerted a direct response in the depth, chlorophyll concentration, and photosynthetic competence of phytoplankton in the SCM. A cyclonic eddy characterized by a high chlorophyll concentration (1.6 mg m-3) and high values of Fv/Fm, (0.52) was detected in the northern zone of the study area. In the central zone, a cyclonic eddy (1.2 mg m-3); and other structure resembling a mode-water eddy was located northwest of Cedros Island. This structure presented the highest chlorophyll concentration (1.8 mg m-3) and high Fv/Fm (?0.5). Chlorophyll concentration and the photosynthetic performance of the phytoplankton community was lower outside of these eddies. Cyanobacteria dominated the phytoplankton SCM community in these areas.

Almazán-Becerril, Antonio; Rivas, David; García-Mendoza, Ernesto



Root endophyte symbiosis in vitro between the ectomycorrhizal basidiomycete Tricholoma matsutake and the arbuscular mycorrhizal plant Prunus speciosa.  


We previously reported that Tricholoma matsutake and Tricholoma fulvocastaneum, ectomycorrhizal basidiomycetes that associate with Pinaceae and Fagaceae, respectively, in the Northern Hemisphere, could interact in vitro as a root endophyte of somatic plants of Cedrela odorata (Meliaceae), which naturally harbors arbuscular mycorrhizal fungi in South America, to form a characteristic rhizospheric colony or "shiro". We questioned whether this phenomenon could have occurred because of plant-microbe interactions between geographically separated species that never encounter one another in nature. In the present study, we document that these fungi formed root endophyte interactions and shiro within 140 days of inoculation with somatic plants of Prunus speciosa (=Cerasus speciosa, Rosaceae), a wild cherry tree that naturally harbors arbuscular mycorrhizal fungi in Japan. Compared with C. odorata, infected P. speciosa plants had less mycelial sheath surrounding the exodermis, and the older the roots, especially main roots, the more hyphae penetrated. In addition, a large number of juvenile roots were not associated with hyphae. We concluded that such root endophyte interactions were not events isolated to the interactions between exotic plants and microbes but could occur generally in vitro. Our pure culture system with a somatic plant allowed these fungi to express symbiosis-related phenotypes that varied with the plant host; these traits are innately programmed but suppressed in nature and could be useful in genetic analyses of plant-fungal symbiosis. PMID:24158697

Murata, Hitoshi; Yamada, Akiyoshi; Yokota, Satoru; Maruyama, Tsuyoshi; Endo, Naoki; Yamamoto, Kohei; Ohira, Tatsuro; Neda, Hitoshi



Oxygen isotopes in tree rings are a good proxy for Amazon precipitation and El Niño-Southern Oscillation variability  

PubMed Central

We present a unique proxy for the reconstruction of variation in precipitation over the Amazon: oxygen isotope ratios in annual rings in tropical cedar (Cedrela odorata). A century-long record from northern Bolivia shows that tree rings preserve the signal of oxygen isotopes in precipitation during the wet season, with weaker influences of temperature and vapor pressure. Tree ring ?18O correlates strongly with ?18O in precipitation from distant stations in the center and west of the basin, and with Andean ice core ?18O showing that the signal is coherent over large areas. The signal correlates most strongly with basin-wide precipitation and Amazon river discharge. We attribute the strength of this (negative) correlation mainly to the cumulative rainout processes of oxygen isotopes (Rayleigh distillation) in air parcels during westward transport across the basin. We further find a clear signature of the El Niño-Southern Oscillation (ENSO) in the record, with strong ENSO influences over recent decades, but weaker influence from 1925 to 1975 indicating decadal scale variation in the controls on the hydrological cycle. The record exhibits a significant increase in ?18O over the 20th century consistent with increases in Andean ?18O ice core and lake records, which we tentatively attribute to increased water vapor transport into the basin. Taking these data together, our record reveals a fresh path to diagnose and improve our understanding of variation and trends of the hydrological cycle of the world’s largest river catchment. PMID:23027960

Brienen, Roel J. W.; Helle, Gerd; Pons, Thijs L.; Guyot, Jean-Loup; Gloor, Manuel



Oxygen isotopes in tree rings are a good proxy for Amazon precipitation and El Nino-Southern Oscillation variability.  


We present a unique proxy for the reconstruction of variation in precipitation over the Amazon: oxygen isotope ratios in annual rings in tropical cedar (Cedrela odorata). A century-long record from northern Bolivia shows that tree rings preserve the signal of oxygen isotopes in precipitation during the wet season, with weaker influences of temperature and vapor pressure. Tree ring ?(18)O correlates strongly with ?(18)O in precipitation from distant stations in the center and west of the basin, and with Andean ice core ?(18)O showing that the signal is coherent over large areas. The signal correlates most strongly with basin-wide precipitation and Amazon river discharge. We attribute the strength of this (negative) correlation mainly to the cumulative rainout processes of oxygen isotopes (Rayleigh distillation) in air parcels during westward transport across the basin. We further find a clear signature of the El Niño-Southern Oscillation (ENSO) in the record, with strong ENSO influences over recent decades, but weaker influence from 1925 to 1975 indicating decadal scale variation in the controls on the hydrological cycle. The record exhibits a significant increase in ?(18)O over the 20th century consistent with increases in Andean ?(18)O ice core and lake records, which we tentatively attribute to increased water vapor transport into the basin. Taking these data together, our record reveals a fresh path to diagnose and improve our understanding of variation and trends of the hydrological cycle of the world's largest river catchment. PMID:23027960

Brienen, Roel J W; Helle, Gerd; Pons, Thijs L; Guyot, Jean-Loup; Gloor, Manuel



Evolution of subsidence styles in forearc basin: example from Cretaceous of southern Vizcaino Peninsula, Baja California, Mexico  

SciTech Connect

Late Jurassic to Early Cretaceous arc magmatism is represented by volcaniclastic rocks of the Eugenia Formation in the northern Vizcaino Peninsula and by the metamorphosed Cedros-San Andres volcanoplutonic complex, with a dismembered ophiolitic basement, in the southern peninsula. The Vizcaino Peninsula became the site of forearc sedimentation by the Aptian-Albian (late Early Cretaceous), when arc magmatism moved abruptly eastward to the present-day Peninsular Range. On the southern Vizcaino Peninsula, a conformable stratigraphic section, complicated by later faulting, records a gradual transition from a ridged forearc, broken by basement uplifts and grabens (the Aptian-Albian Asunction Formation), to a broadly subsiding, deep marine forearc basin (the Cenomanian Valle Formation). The basal contact of the Asunction formation has irregular relief caused by brecciated basement rocks and talus accumulated along fault zones. An upward-fining sequence several hundred meters thick records abrupt uplift and gradual denudation of adjacent metamorphic basement. Contemporaneous andesite arc volcanism to the east supplied ash and fresh volcanic detritus to the grabens. Angular sand to boulder-size detritus of the Asunction Formation was derived locally, and includes basic to intermediate meta-igneous rock fragments, with epidote, actinolite, and chlorite, as well as serpentine. Abundant calcareous fossils are commonly unbroken, suggesting local sources for these as well. Angular to subrounded, sand to cobble-sized, intermediate to mafic volcanic rock fragments were derived from a more distant island arc to the east, which occasionally provided intermediate to felsic tuffs to the basin. This source is probably represented by the Aptian-Albian Alisitos Group, which forms much of the western wall of the Late Cretaceous Peninsular Range batholith.

Busby-Spera, C.J.; Boles, J.R.



Antileishmanial, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants.  


Extracts (34) from eight plant species of the Peruvian Amazonia currently used in traditional Peruvian medicine, mostly as antileishmanial remedies and also as painkiller, antiseptic, antipyretic, anti-inflamatory, antiflu, astringent, diuretic, antipoison, anticancerous, antiparasitic, insecticidal, or healing agents, have been tested for their antileishmanial, antitrypanosomal, and cytotoxic activity. Plant species were selected based on interviews conducted with residents of rural areas. The different plant parts were dried, powdered, and extracted by maceration with different solvents (hexane, chloroform, and 70% ethanol-water). These extracts were tested on promastigote forms of Leishmania infantum strain PB75, epimastigote forms of Trypanosoma cruzi strain Y, and the mammalian CHO cell line. Parasite viability and nonspecific cytotoxicity were analyzed by a modified MTT colorimetric assay method. The isolation and identification of pure compounds from selected extracts were performed by column chromatography, gas chromatography mass spectrometry (GC-MS; mixtures), spectroscopic techniques [MS, infrared (IR), ultraviolet (UV)], and mono and two-dimensional (1)H and (13)C nuclear magnetic resonance (NMR; COSY, HSQC, NOESY) experiments. Chondodendron tomentosum bark and Cedrela odorata were the most active extracts against Leishmania, while C. odorata and Aristoloquia pilosa were the most active against Trypanosoma, followed by Tabebuia serratifolia, Tradescantia zebrina, and Zamia ulei. Six compounds and two mixtures were isolated from Z. ulei [cycasin (1)], T. serratifolia {mixtures 1-2, and naphthoquinones 2-acetyl-4H,9H-naphtho[2,3-b]furan-4,9-dione (2) and 2-(1-hydroxyethyl)-4H,9H-naphtho[2,3-b]furan-4,9-dione (3)}, and C. tomentosum [chondrocurine (4); (S,S')-12-O-methyl(+)-curine (5); and cycleanine (6)]. Four compounds and the two mixtures exhibited significant activity. PMID:21922239

González-Coloma, Azucena; Reina, Matías; Sáenz, Claudia; Lacret, Rodney; Ruiz-Mesia, Lastenia; Arán, Vicente J; Sanz, Jesús; Martínez-Díaz, Rafael A



Assessing the extent of "conflict of use" in multipurpose tropical forest trees: a regional view.  


In the context of multiple forest management, multipurpose tree species which provide both timber and non-timber forest products (NTFP), present particular challenges as the potential of conflicting use for either product may be high. One key aspect is that the magnitude of conflict of use can be location specific, thus adding complexity to policy development. This paper focuses on the extent to which the potential for conflict of use in multipurpose tree species varies across the Amazonian lowland forests shared by Peru, Bolivia, Colombia, Ecuador and Venezuela, emphasizing the economic dimension of conflict. Based on a review of the current normative and regulatory aspects of timber and NTFP extraction in the five countries, the paper also briefly discusses the opportunities and constraints for harmonization of timber and NTFP management of multipurpose species across the region. It was found that about half of the 336 timber species reviewed across the five countries also have non-timber uses. Eleven timber species are multipurpose in all five countries: Calophyllum brasiliense, Cedrela odorata, Ceiba pentandra, Clarisia racemosa, Ficus insipida, Jacaranda copaia, Schefflera morototoni, Simarouba amara and Terminalia amazonia. Seven other multipurpose species occurred only in either Venezuela (Tabebuia impetiginosa, Spondias mombin, Pentaclethra macroloba, Copaifera officinalis, Chlorophora tinctoria, Carapa guianensis) or Ecuador (Tabebuia chrysantha). Four multipurpose tree species presented the highest potential of conflict of use across the region: Dipteryx odorata, Tabebuia serratifolia, Hymenaea courbaril and Myroxylon balsamum yet these were not evenly distributed across all five countries. None of the five studied countries have specific legislation to promote sustainable use of any of the multipurpose species reported here and thus mitigate potential conflict of use; nor documented management options for integration or else segregation of both their timber and NTFP values. PMID:24061084

Herrero-Jáuregui, Cristina; Guariguata, Manuel R; Cárdenas, Dairon; Vilanova, Emilio; Robles, Marco; Licona, Juan Carlos; Nalvarte, Walter



An efficient immunodetection method for histone modifications in plants  

PubMed Central

Background Epigenetic mechanisms can be highly dynamic, but the cross-talk among them and with the genome is still poorly understood. Many of these mechanisms work at different places in the cell and at different times of organism development. Covalent histone modifications are one of the most complex and studied epigenetic mechanisms involved in cellular reprogramming and development in plants. Therefore, the knowledge of the spatial distribution of histone methylation in different tissues is important to understand their behavior on specific cells. Results Based on the importance of epigenetic marks for biology, we present a simplified, inexpensive and efficient protocol for in situ immunolocalization on different tissues such as flowers, buds, callus, somatic embryo and meristematic tissue from several plants of agronomical and biological importance. Here, we fully describe all the steps to perform the localization of histone modifications. Using this method, we were able to visualize the distribution of H3K4me3 and H3K9me2 without loss of histological integrity of tissues from several plants, including Agave tequilana, Capsicum chinense, Coffea canephora and Cedrela odorata, as well as Arabidopsis thaliana. Conclusions There are many protocols to study chromatin modifications; however, most of them are expensive, difficult and require sophisticated equipment. Here, we provide an efficient protocol for in situ localization of histone methylation that dispenses with the use of expensive and sensitive enzymes. The present method can be used to investigate the cellular distribution and localization of a wide array of proteins, which could help to clarify the biological role that they play at specific times and places in different tissues of various plant species. PMID:24341414



Complementary soil water use is indicated in mixed native tree plantations, Panama  

NASA Astrophysics Data System (ADS)

Recent approaches to reforestation in the tropics emphasize the establishment of mixed species plantations to provide production but also ecological benefits. However, little is known about the effects of mixed-species stands on soil water utilization und tree water use rates. The objectives of this study were i) to asses seasonal plant water uptake patterns for different species and ii) to examine the effects of species diversity on water uptake and water use rates. The study was conducted in a 7-yr-old plantation in Panama. Five native tree species were planted in mono-, three- and five-species plots. Natural abundance stable isotopic ratios of hydrogen (?2H) and oxygen (?18O) in xylem and soil water samples were used to determine water uptake patterns. The contribution of water from each soil layer to xylem water was calculated using the isotope mixing model SISUS (Stable Isotope Sourcing Using Sampling). Sap flux was measured using thermal dissipation probes. Isotope ratios in soil and xylem water fell below the local meteoric water line (LMWL) suggesting some evaporation. Soil water isotope ratio increased with depth from an average of -18 ‰ and -1 ‰ in the surface soil layer (0-10 cm) to -40 ‰ and -8 ‰ at 50-70 cm depth for ?2H and ?18O, respectively. During the dry season ?2H and ?18O of xylem water were negatively correlated with tree water use rates suggesting that trees taking up water from deeper soil horizons were able to maintain higher water use rates. Species tended to differ in their water uptake pattern, especially during the dry season. Cedrela odorata and Hura crepitans were primarily obtaining water (60 to 80%) from the upper 30 cm. In contrast, Tabebuia rosea took up most of its water (75-90%) from soil depths > 30 cm. Xylem water ?2H and ?18O in Anacardium excelsum and Luehea seemannii suggest that water was taken up to equal proportions from all soil horizons. In mixed stands, overall transpirational water use was higher than in mono-specific stands, which can partly be explained by complementary soil water use among the coexisting tree species.

Schwendenmann, L.; Sánchez Bragado, R.; Kunert, N.; Hölscher, D.



Water use in four model tropical plant associations established in the lowlands of Costa Rica.  


We examined soil water use patterns of four model plant associations established in the North Caribbean lowlands of Costa Rica by comparing the stable hydrogen isotope composition, deltaD, in xylem sap and in soil water at different depths, under rainy and dry conditions. Four 5-year-old model plant associations composed of 2 tree species (Hyeronima alchorneoides and Cedrela odorata) having different architecture and phenology were studied. Average tree height was 8.9 and 7.6 m, respectively. Each tree species was grown in monoculture and in polyculture with 2 perennial monocotyledons (Euterpe oleracea and Heliconia imbricata). Maximum rooting depth at the time of 6D determination was approximately 2 m for almost all species. Most roots of all species were concentrated in the upper soil layers. Stomatal conductance to water vapor (gS) was higher in the deciduous C. odorata than in the evergreen H. alchorneoides; within each species, g, did not differ when the trees were grown in mono or in polyculture. During the rainy season, gradients in soil water 6D were not observed. Average rainy season xylem sap deltaD did not differ among members of the plant combinations tested (-30% per thousand), and was more similar to deltaD values of shallow soil water. Under dry conditions, volumetric soil water content declined from 50 to approximately 35%, and modest gradients in soil water deltaD were observed. Xylem sap deltaD obtained during dry conditions was significantly lower than rainy season values. Xylem sap deltaD of plants growing in the four associations varied between -9 and -22% per hundred, indicating that shallow water was predominantly absorbed during the dry period too. Differences in xylem sap deltaD of trees and monocots were also detected, but no significant patterns emerged. The results suggest that: (a) the plant associations examined extracted water predominantly from shallow soil layers (<1 m), (b) the natural isotopic variation in soil and plant water at the study site was low, and (c) the plant mixes obtain water from more than a single soil layer simultaneously. Temporal factors were important in determining the competition and complementary relations observed among the trees and the perennial monocots. Under the prevailing environmental conditions, water use in these plant associations was determined largely by species-specific attributes such as biomass allocation to fine roots, phenology, and canopy architecture, and to a lesser extent by water limitations. PMID:19419093

Gutiérrez-Soto, Marco V; Ewel, John J



Species and rotation frequency influence soil nitrogen in simplified tropical plant communities.  


Among the many factors that potentially influence the rate at which nitrogen (N) becomes available to plants in terrestrial ecosystems are the identity and diversity of species composition, frequency of disturbance or stand turnover, and time. Replicated suites of investigator-designed communities afforded an opportunity to examine the effects of those factors on net N mineralization over a 12-year period. The communities consisted of large-stature perennial plants, comprising three tree species (Hyeronima alchorneoides, Cedrela odorata, and Cordia alliodora), a palm (Euterpe oleracea), and a large, perennial herb (Heliconia imbricata). Trees were grown in monoculture and in combination with the other two life-forms; tree monocultures were subjected to rotations of one or four years, or like the three-life-form systems, left uncut. The work was conducted on fertile soil in the humid lowlands of Costa Rica, a site with few abiotic constraints to plant growth. Rates of net N mineralization and nitrification were high, typically in the range of 0.2-0.8 microg x g(1) x d(-1), with net nitrification slightly higher than net mineralization, indicating preferential uptake of ammonium (NH4+) by plants and microbes. Net rates of N mineralization were about 30% lower in stands of one of the three tree species, Hyeronima, than in stands of the other two. Contrary to expectations, short-rotation management (one or four years) resulted in higher net rates of N mineralization than in uncut stands, whether the latter were composed of a single tree species or a combination of life-forms. Neither additional species richness nor replenishment of leached N augmented mineralization rates. The net rate at which N was supplied tended to be lowest in stands where demand for N was highest. Careful choice of species, coupled with low frequency of disturbance, can lead to maintenance of N within biomass and steady rates of within-system circulation, whereas pulses, whether caused by cutting and replanting or by the phenological traits of the species selected or combined, subject N supplies to leaching loss. PMID:16711039

Ewel, John J