Characterization and isolation of highly purified porcine satellite cells

PubMed Central

Ding, Shijie; Wang, Fei; Liu, Yan; Li, Sheng; Zhou, Guanghong; Hu, Ping

2017-01-01

Pig is an important food source and an excellent system to model human diseases. Careful characterization of the swine skeletal muscle stem cells (satellite cells) will shed lights on generation of swine skeletal muscle disease model and efficient production of porcine meat for the food industry. Paired box protein 7 (Pax7) is a highly conserved transcription factor shared by satellite cells from various species. However, the sequence of Pax7 has not been characterized in pig. The lack of method to isolate highly purified satellite cells hinders the thorough characterization of the swine satellite cells. Here we found molecular markers for swine satellite cells and revealed that the porcine satellite cells were heterogeneous in various pieces of skeletal muscle. We further developed a method to isolate highly purified satellite cells directly from porcine muscles using fluorescence-activated cell sorting. We next characterized the proliferation and differentiation abilities of isolated satellite cells in vitro; and found that long-term culturing of satellite cells in vitro led to stemness loss. PMID:28417015

Satellite cell therapy – from mice to men

PubMed Central

2013-01-01

Satellite cells are rare mononuclear skeletal muscle-resident cells that are the chief contributors to regenerative myogenesis following muscle injury. Although first identified more than 50 years ago, it is only recently that the murine satellite cell has become molecularly defined with the ability to prospectively isolate these cells from their niche. Human satellite cells are considerably less well understood with relatively few studies having been performed on them. In this review, a critical evaluation of this literature is provided along with a discussion of the practical and methodological issues involved with research on human satellite cells. The therapeutic potential of these and other cells types is also discussed, and the various challenges that face satellite cell therapy are addressed. PMID:23369649

Satellite Cells in Muscular Dystrophy - Lost in Polarity

PubMed Central

Chang, Natasha C.; Chevalier, Fabien P.; Rudnicki, Michael A.

2016-01-01

Recent findings employing the mdx mouse model for Duchenne muscular dystrophy (DMD) have revealed that muscle satellite stem cells play a direct role in contributing to disease etiology and progression of DMD, the most common and severe form of muscular dystrophy. Lack of dystrophin expression in DMD has critical consequences in satellite cells including an inability to establish cell polarity, abrogation of asymmetric satellite stem cell divisions and failure to enter the myogenic program. Thus, muscle wasting in dystrophic mice is not only caused by myofiber fragility, but is exacerbated by intrinsic satellite cell dysfunction leading to impaired regeneration. Despite intense research and clinical efforts, there is still no effective cure for DMD. In this review, we highlight recent research advances in DMD and discuss the current state of treatment and importantly, how we can incorporate satellite cell-targeted therapeutic strategies to correct satellite cell dysfunction in DMD. PMID:27161598

Skeletal muscle satellite cells cultured in simulated microgravity

NASA Technical Reports Server (NTRS)

Molnar, Greg; Hartzell, Charles R.; Schroedl, Nancy A.; Gonda, Steve R.

1993-01-01

Satellite cells are postnatal myoblasts responsible for providing additional nuclei to growing or regenerating muscle cells. Satellite cells retain the capacity to proliferate and differentiate in vitro and therefore provide a useful model to study postnatal muscle development. Most culture systems used to study postnatal muscle development are limited by the two-dimensional (2-D) confines of the culture dish. Limiting proliferation and differentiation of satellite cells in 2-D could potentially limit cell-cell contacts important for developing the level of organization in skeletal muscle obtained in vivo. Culturing satellite cells on microcarrier beads suspended in the High-Aspect-Ratio-Vessel (HARV) designed by NASA provides a low shear, three-dimensional (3-D) environment to study muscle development. Primary cultures established from anterior tibialis muscles of growing rats (approximately 200 gm) were used for all studies and were composed of greater than 75 % satellite cells. Different inoculation densities did not affect the proliferative potential of satellite cells in the HARV. Plating efficiency, proliferation, and glucose utilization were compared between 2-D flat culture and 3-D HARV culture. Plating efficiency (cells attached - cells plated x 100) was similar between the two culture systems. Proliferation was reduced in HARV cultures and this reduction was apparent for both satellite cells and non-satellite cells. Furthermore, reduction in proliferation within the HARV could not be attributed to reduced substrate availability since glucose levels in media from HARV and 2-D cell culture were similar. Morphologically, microcarrier beads within the HARVS were joined together by cells into three-dimensional aggregates composed of greater than 10 beads/aggregate. Aggregation of beads did not occur in the absence of cells. Myotubes were often seen on individual beads or spanning the surface of two beads. In summary, proliferation and differentiation of

New Solar Cells to Boost Satellite Power

Science.gov Websites

New Solar Cells to Boost Satellite Power For more information contact: George Douglas (303) 275 much as doubled, each satellite and its services can be more economical and offer a greater return to satellite customers. The new cells will also have a longer useful life. Because of their construction they

Fibronectin regulates Wnt7a signaling and satellite cell expansion

PubMed Central

Bentzinger, C. Florian; Wang, Yu Xin; von Maltzahn, Julia; Soleimani, Vahab D.; Yin, Hang; Rudnicki, Michael A.

2012-01-01

SUMMARY The influence of the extracellular matrix (ECM) within the stem cell niche remains poorly understood. We found that Syndecan-4 (Sdc4) and Frizzled-7 (Fzd7) form a co-receptor complex in satellite cells and that binding of the ECM glycoprotein Fibronectin (FN) to Sdc4 stimulates the ability of Wnt7a to induce the symmetric expansion of satellite stem cells. Newly activated satellite cells dynamically remodel their niche by transient high-level expression of FN. Knockdown of FN in prospectively isolated satellite cells severely impaired their ability to repopulate the satellite cell niche. Conversely, in vivo over-expression of FN with Wnt7a dramatically stimulated the expansion of satellite stem cells in regenerating muscle. Therefore, activating satellite cells remodel their niche through autologous expression of FN that provides feedback to stimulate Wnt7a signaling through the Fzd7/Sdc4 co-receptor complex. Thus, FN and Wnt7a together regulate the homeostatic levels of satellite stem cells and satellite myogenic cells during regenerative myogenesis. PMID:23290138

TAK1 modulates satellite stem cell homeostasis and skeletal muscle repair

PubMed Central

Ogura, Yuji; Hindi, Sajedah M.; Sato, Shuichi; Xiong, Guangyan; Akira, Shizuo; Kumar, Ashok

2015-01-01

Satellite cells are resident adult stem cells that are required for regeneration of skeletal muscle. However, signalling mechanisms that regulate satellite cell function are less understood. Here we demonstrate that transforming growth factor-β-activated kinase 1 (TAK1) is important in satellite stem cell homeostasis and function. Inactivation of TAK1 in satellite cells inhibits muscle regeneration in adult mice. TAK1 is essential for satellite cell proliferation and its inactivation causes precocious differentiation. Moreover, TAK1-deficient satellite cells exhibit increased oxidative stress and undergo spontaneous cell death, primarily through necroptosis. TAK1 is required for the activation of NF-κB and JNK in satellite cells. Forced activation of NF-κB improves survival and proliferation of TAK1-deficient satellite cells. Furthermore, TAK1-mediated activation of JNK is essential to prevent oxidative stress and precocious differentiation of satellite cells. Collectively, our study suggests that TAK1 is required for maintaining the pool of satellite stem cells and for regenerative myogenesis. PMID:26648529

Effective fiber hypertrophy in satellite cell-depleted skeletal muscle

PubMed Central

McCarthy, John J.; Mula, Jyothi; Miyazaki, Mitsunori; Erfani, Rod; Garrison, Kelcye; Farooqui, Amreen B.; Srikuea, Ratchakrit; Lawson, Benjamin A.; Grimes, Barry; Keller, Charles; Van Zant, Gary; Campbell, Kenneth S.; Esser, Karyn A.; Dupont-Versteegden, Esther E.; Peterson, Charlotte A.

2011-01-01

An important unresolved question in skeletal muscle plasticity is whether satellite cells are necessary for muscle fiber hypertrophy. To address this issue, a novel mouse strain (Pax7-DTA) was created which enabled the conditional ablation of >90% of satellite cells in mature skeletal muscle following tamoxifen administration. To test the hypothesis that satellite cells are necessary for skeletal muscle hypertrophy, the plantaris muscle of adult Pax7-DTA mice was subjected to mechanical overload by surgical removal of the synergist muscle. Following two weeks of overload, satellite cell-depleted muscle showed the same increases in muscle mass (approximately twofold) and fiber cross-sectional area with hypertrophy as observed in the vehicle-treated group. The typical increase in myonuclei with hypertrophy was absent in satellite cell-depleted fibers, resulting in expansion of the myonuclear domain. Consistent with lack of nuclear addition to enlarged fibers, long-term BrdU labeling showed a significant reduction in the number of BrdU-positive myonuclei in satellite cell-depleted muscle compared with vehicle-treated muscle. Single fiber functional analyses showed no difference in specific force, Ca2+ sensitivity, rate of cross-bridge cycling and cooperativity between hypertrophied fibers from vehicle and tamoxifen-treated groups. Although a small component of the hypertrophic response, both fiber hyperplasia and regeneration were significantly blunted following satellite cell depletion, indicating a distinct requirement for satellite cells during these processes. These results provide convincing evidence that skeletal muscle fibers are capable of mounting a robust hypertrophic response to mechanical overload that is not dependent on satellite cells. PMID:21828094

Tissue-specific stem cells: Lessons from the skeletal muscle satellite cell

PubMed Central

Brack, Andrew S.; Rando, Thomas A.

2012-01-01

In 1961, the satellite cell was first identified when electron microscopic examination of skeletal muscle demonstrated a cell wedged between the plasma membrane of the muscle fiber and the basement membrane. In recent years it has been conclusively demonstrated that the satellite cell is the primary cellular source for muscle regeneration and is equipped with the potential to self renew, thus functioning as a bone fide skeletal muscle stem cell (MuSC). As we move past the 50th anniversary of the satellite cell, we take this opportunity to discuss the current state of the art and dissect the unknowns in the MuSC field. PMID:22560074

Chronic inflammation in skeletal muscle impairs satellite cells function during regeneration: can physical exercise restore the satellite cell niche?

PubMed

Perandini, Luiz Augusto; Chimin, Patricia; Lutkemeyer, Diego da Silva; Câmara, Niels Olsen Saraiva

2018-06-01

Chronic inflammation impairs skeletal muscle regeneration. Although many cells are involved in chronic inflammation, macrophages seem to play an important role in impaired muscle regeneration since these cells are associated with skeletal muscle stem cell (namely, satellite cells) activation and fibro-adipogenic progenitor cell (FAP) survival. Specifically, an imbalance of M1 and M2 macrophages seems to lead to impaired satellite cell activation, and these are the main cells that function during skeletal muscle regeneration, after muscle damage. Additionally, this imbalance leads to the accumulation of FAPs in skeletal muscle, with aberrant production of pro-fibrotic factors (e.g., extracellular matrix components), impairing the niche for proper satellite cell activation and differentiation. Treatments aiming to block the inflammatory pro-fibrotic response are partially effective due to their side effects. Therefore, strategies reverting chronic inflammation into a pro-regenerative pattern are required. In this review, we first describe skeletal muscle resident macrophage ontogeny and homeostasis, and explain how macrophages are replenished after muscle injury. We next discuss the potential role of chronic physical activity and exercise in restoring the M1 and M2 macrophage balance and consequently, the satellite cell niche to improve skeletal muscle regeneration after injury. © 2018 Federation of European Biochemical Societies.

Protein Availability and Satellite Cell Dynamics in Skeletal Muscle.

PubMed

Shamim, Baubak; Hawley, John A; Camera, Donny M

2018-06-01

Human skeletal muscle satellite cells are activated in response to both resistance and endurance exercise. It was initially proposed that satellite cell proliferation and differentiation were only required to support resistance exercise-induced hypertrophy. However, satellite cells may also play a role in muscle fibre remodelling after endurance-based exercise and extracellular matrix regulation. Given the importance of dietary protein, particularly branched chain amino acids, in supporting myofibrillar and mitochondrial adaptations to both resistance and endurance-based training, a greater understanding of how protein intake impacts satellite cell activity would provide further insight into the mechanisms governing skeletal muscle remodelling with exercise. While many studies have investigated the capacity for protein ingestion to increase post-exercise rates of muscle protein synthesis, few investigations have examined the role for protein ingestion to modulate satellite cell activity. Here we review the molecular mechanisms controlling the activation of satellite cells in response to mechanical stress and protein intake in both in vitro and in vivo models. We provide a mechanistic framework that describes how protein ingestion may enhance satellite activity and promote exercise adaptations in human skeletal muscle.

A role for RNA post-transcriptional regulation in satellite cell activation

PubMed Central

2012-01-01

Background Satellite cells are resident skeletal muscle stem cells responsible for muscle maintenance and repair. In resting muscle, satellite cells are maintained in a quiescent state. Satellite cell activation induces the myogenic commitment factor, MyoD, and cell cycle entry to facilitate transition to a population of proliferating myoblasts that eventually exit the cycle and regenerate muscle tissue. The molecular mechanism involved in the transition of a quiescent satellite cell to a transit-amplifying myoblast is poorly understood. Methods Satellite cells isolated by FACS from uninjured skeletal muscle and 12 h post-muscle injury from wild type and Syndecan-4 null mice were probed using Affymetrix 430v2 gene chips and analyzed by Spotfiretm and Ingenuity Pathway analysis to identify gene expression changes and networks associated with satellite cell activation, respectively. Additional analyses of target genes identify miRNAs exhibiting dynamic changes in expression during satellite cell activation. The function of the miRNAs was assessed using miRIDIAN hairpin inhibitors. Results An unbiased gene expression screen identified over 4,000 genes differentially expressed in satellite cells in vivo within 12 h following muscle damage and more than 50% of these decrease dramatically. RNA binding proteins and genes involved in post-transcriptional regulation were significantly over-represented whereas splicing factors were preferentially downregulated and mRNA stability genes preferentially upregulated. Furthermore, six computationally identified miRNAs demonstrated novel expression through muscle regeneration and in satellite cells. Three of the six miRNAs were found to regulate satellite cell fate. Conclusions The quiescent satellite cell is actively maintained in a state poised to activate in response to external signals. Satellite cell activation appears to be regulated by post-transcriptional gene regulation. PMID:23046558

Muscle Satellite Cell Protein Teneurin‐4 Regulates Differentiation During Muscle Regeneration

PubMed Central

Ishii, Kana; Suzuki, Nobuharu; Mabuchi, Yo; Ito, Naoki; Kikura, Naomi; Fukada, So‐ichiro; Okano, Hideyuki; Takeda, Shin'ichi

2015-01-01

Abstract Satellite cells are maintained in an undifferentiated quiescent state, but during muscle regeneration they acquire an activated stage, and initiate to proliferate and differentiate as myoblasts. The transmembrane protein teneurin‐4 (Ten‐4) is specifically expressed in the quiescent satellite cells; however, its cellular and molecular functions remain unknown. We therefore aimed to elucidate the function of Ten‐4 in muscle satellite cells. In the tibialis anterior (TA) muscle of Ten‐4‐deficient mice, the number and the size of myofibers, as well as the population of satellite cells, were reduced with/without induction of muscle regeneration. Furthermore, we found an accelerated activation of satellite cells in the regenerated Ten‐4‐deficient TA muscle. The cell culture analysis using primary satellite cells showed that Ten‐4 suppressed the progression of myogenic differentiation. Together, our findings revealed that Ten‐4 functions as a crucial player in maintaining the quiescence of muscle satellite cells. Stem Cells 2015;33:3017–3027 PMID:26013034

Use of Advanced Solar Cells for Commercial Communication Satellites

NASA Technical Reports Server (NTRS)

Bailey, Sheila G.; Landis, Geoffrey A.

1995-01-01

The current generation of communications satellites are located primarily in geosynchronous Earth orbit (GEO). Over the next decade, however, a new generation of communications satellites will be built and launched, designed to provide a world-wide interconnection of portable telephones. For this mission, the satellites must be positioned in lower polar and near-polar orbits. To provide complete coverage, large numbers of satellites will be required. Because the required number of satellites decreases as the orbital altitude is increased, fewer satellites would be required if the orbit chosen were raised from low to intermediate orbit. However, in intermediate orbits, satellites encounter significant radiation due to trapped electrons and protons. Radiation tolerant solar cells may be necessary to make such satellites feasible. We analyze the amount of radiation encountered in low and intermediate polar orbits at altitudes of interest to next-generation communication satellites, calculate the expected degradation for silicon, GaAs, and InP solar cells, and show that the lifetimes can be significantly increased by use of advanced solar cells.

Use of advanced solar cells for commerical communication satellites

NASA Astrophysics Data System (ADS)

Landis, Geoffrey A.; Bailey, Sheila G.

1995-01-01

The current generation of communications satellites are located primarily in geosynchronous Earth orbit (GEO). Over the next decade, however, a new generation of communications satellites will be built and launched, designed to provide a world-wide interconnection of portable telephones. For this mission, the satellites must be positioned in lower polar- and near-polar orbits. To provide complete coverage, large numbers of satellites will be required. Because of the required number of satellites decreases as the orbital altitude is increased, fewer satellites would be required if the orbit chosen were raised from Low to intermediate orbit. However, in intermediate orbits, satellites encounter significant radiation due to trapped electrons and protons. Radiation tolerant solar cells may be necessary to make such satellites feasible. We analyze the amount of radiation encountered in low and intermediate polar orbits at altitudes of interest to next-generation communication satellites, calculate the expected degradation for silicon, GaAs, and InP solar cells, and show that the lifetimes can be significantly increased by use of advanced solar cells.

Use of advanced solar cells for commercial communication satellites

NASA Astrophysics Data System (ADS)

Bailey, Sheila G.; Landis, Geoffrey A.

1995-03-01

The current generation of communications satellites are located primarily in geosynchronous Earth orbit (GEO). Over the next decade, however, a new generation of communications satellites will be built and launched, designed to provide a world-wide interconnection of portable telephones. For this mission, the satellites must be positioned in lower polar and near-polar orbits. To provide complete coverage, large numbers of satellites will be required. Because the required number of satellites decreases as the orbital altitude is increased, fewer satellites would be required if the orbit chosen were raised from low to intermediate orbit. However, in intermediate orbits, satellites encounter significant radiation due to trapped electrons and protons. Radiation tolerant solar cells may be necessary to make such satellites feasible. We analyze the amount of radiation encountered in low and intermediate polar orbits at altitudes of interest to next-generation communication satellites, calculate the expected degradation for silicon, GaAs, and InP solar cells, and show that the lifetimes can be significantly increased by use of advanced solar cells.

Notch signaling deficiency underlies age-dependent depletion of satellite cells in muscular dystrophy

PubMed Central

Jiang, Chunhui; Wen, Yefei; Kuroda, Kazuki; Hannon, Kevin; Rudnicki, Michael A.; Kuang, Shihuan

2014-01-01

Duchenne muscular dystrophy (DMD) is a devastating disease characterized by muscle wasting, loss of mobility and death in early adulthood. Satellite cells are muscle-resident stem cells responsible for the repair and regeneration of damaged muscles. One pathological feature of DMD is the progressive depletion of satellite cells, leading to the failure of muscle repair. Here, we attempted to explore the molecular mechanisms underlying satellite cell ablation in the dystrophin mutant mdx mouse, a well-established model for DMD. Initial muscle degeneration activates satellite cells, resulting in increased satellite cell number in young mdx mice. This is followed by rapid loss of satellite cells with age due to the reduced self-renewal ability of mdx satellite cells. In addition, satellite cell composition is altered even in young mdx mice, with significant reductions in the abundance of non-committed (Pax7+ and Myf5−) satellite cells. Using a Notch-reporter mouse, we found that the mdx satellite cells have reduced activation of Notch signaling, which has been shown to be necessary to maintain satellite cell quiescence and self-renewal. Concomitantly, the expression of Notch1, Notch3, Jag1, Hey1 and HeyL are reduced in the mdx primary myoblast. Finally, we established a mouse model to constitutively activate Notch signaling in satellite cells, and show that Notch activation is sufficient to rescue the self-renewal deficiencies of mdx satellite cells. These results demonstrate that Notch signaling is essential for maintaining the satellite cell pool and that its deficiency leads to depletion of satellite cells in DMD. PMID:24906372

Nandrolone decanoate increases satellite cell numbers in the chicken pectoralis muscle.

PubMed

Allouh, Mohammed Z; Rosser, Benjamin W C

2010-02-01

The anabolic androgenic steroid nandrolone decanoate has minimal androgenic effects and, thus, is widely used to induce muscle hypertrophy in both patients and athletes. Although increases in satellite cell numbers and satellite cells giving rise to new myonuclei are associated with hypertrophy in many experimental models, the relationship between nandrolone and satellite cells is poorly understood. Here we test the hypothesis that nandrolone administration is associated with an increase in satellite cell numbers in muscle. Nandrolone was injected at weekly intervals for four weeks into the right pectoralis muscle of female white leghorn chickens aged 63 days post hatch. Age/size/sex matched control birds received saline injections. The contralateral pectoralis was excised for study from each control and nandrolone treated bird. An antibody against Pax7 and immunocytochemical techniques were used to identify satellite cells. Nandrolone significantly increased mean pectoralis mass by approximately 22%, and mean fiber diameter by about 24%. All satellite cell indices that were quantified increased significantly in chicken pectoralis with administration of nandrolone. Nandrolone injected birds had on average higher satellite cell frequencies (#SC nuclei/all nuclei within basal lamina), number of satellite cells per millimeter of fiber, and satellite cell concentrations (closer together). Myonuclei were further apart (less concentrated) in nandrolone injected muscle. However, an overall increase in myonuclear numbers was revealed by a significantly greater mean number of myonuclei per millimeter of fiber in nandrolone injected muscle. Our results suggest that satellite cells may be key cellular vectors for nandrolone induced muscle fiber hypertrophy.

Human Satellite Cell Transplantation and Regeneration from Diverse Skeletal Muscles

PubMed Central

Xu, Xiaoti; Wilschut, Karlijn J.; Kouklis, Gayle; Tian, Hua; Hesse, Robert; Garland, Catharine; Sbitany, Hani; Hansen, Scott; Seth, Rahul; Knott, P. Daniel; Hoffman, William Y.; Pomerantz, Jason H.

2015-01-01

Summary Identification of human satellite cells that fulfill muscle stem cell criteria is an unmet need in regenerative medicine. This hurdle limits understanding how closely muscle stem cell properties are conserved among mice and humans and hampers translational efforts in muscle regeneration. Here, we report that PAX7 satellite cells exist at a consistent frequency of 2–4 cells/mm of fiber in muscles of the human trunk, limbs, and head. Xenotransplantation into mice of 50–70 fiber-associated, or 1,000–5,000 FACS-enriched CD56+/CD29+ human satellite cells led to stable engraftment and formation of human-derived myofibers. Human cells with characteristic PAX7, CD56, and CD29 expression patterns populated the satellite cell niche beneath the basal lamina on the periphery of regenerated fibers. After additional injury, transplanted satellite cells robustly regenerated to form hundreds of human-derived fibers. Together, these findings conclusively delineate a source of bona-fide endogenous human muscle stem cells that will aid development of clinical applications. PMID:26352798

Isolation and Purification of Satellite Cells for Skeletal Muscle Tissue Engineering

PubMed Central

Syverud, Brian C; Lee, Jonah D; VanDusen, Keith W; Larkin, Lisa M

2015-01-01

Engineered skeletal muscle holds promise as a source of graft tissue for the repair of traumatic injuries such as volumetric muscle loss. The resident skeletal muscle stem cell, the satellite cell, has been identified as an ideal progenitor for tissue engineering due to its role as an essential player in the potent skeletal muscle regeneration mechanism. A significant challenge facing tissue engineers, however, is the isolation of sufficiently large satellite cell populations with high purity. The two common isolation techniques, single fiber explant culture and enzymatic dissociation, can yield either a highly pure satellite cell population or a suitably large number or cells but fail to do both simultaneously. As a result, it is often necessary to use a purification technique such as pre-plating or cell sorting to enrich the satellite cell population post-isolation. Furthermore, the absence of complex chemical and biophysical cues influencing the in vivo satellite cell “niche” complicates the culture of isolated satellite cells. Techniques under investigation to maximize myogenic proliferation and differentiation in vitro are described in this article, along with current methods for isolating and purifying satellite cells. PMID:26413555

Asymmetric Distribution of Primary Cilia Allocates Satellite Cells for Self-Renewal.

PubMed

Jaafar Marican, Nur Hayati; Cruz-Migoni, Sara B; Borycki, Anne-Gaëlle

2016-06-14

Regeneration of vertebrate skeletal muscles requires satellite cells, a population of stem cells that are quiescent in normal conditions and divide, differentiate, and self-renew upon activation triggered by exercise, injury, and degenerative diseases. Satellite cell self-renewal is essential for long-term tissue homeostasis, and previous work has identified a number of external cues that control this process. However, little is known of the possible intrinsic control mechanisms of satellite cell self-renewal. Here, we show that quiescent satellite cells harbor a primary cilium, which is rapidly disassembled upon entry into the cell cycle. Contrasting with a commonly accepted belief, cilia reassembly does not occur uniformly in cells exiting the cell cycle. We found that primary cilia reassemble preferentially in cells committed to self-renew, and disruption of cilia reassembly causes a specific deficit in self-renewing satellite cells. These observations indicate that primary cilia provide an intrinsic cue essential for satellite cell self-renewal. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Optimizing Call Patterns for Landline and Cell Phone Surveys.

PubMed

Reimer, Becky; Roth, Veronica; Montgomery, Robert

2012-01-01

Cell phone surveys have become increasingly popular and researchers have noted major challenges in conducting cost-effective surveys while achieving high response rates. Previous work has shown that calling strategies that maximize both respondent contact and completed interviews for landline surveys may not be the most cost-effective for cell phone surveys. For example, Montgomery, et al. (2011) found important differences between landline and cell samples for best times to call and declines in contact rates after repeated dialing. Using paradata from the 2010 and 2011 National Flu Surveys (sponsored by the Centers for Disease Control and Prevention), we investigate differences in calling outcomes between landline and cell surveys. Specifically, we predict respondent contact and interview completion using logistic regression models that examine the impact of calling on particular days of the week, certain times of the day, number of previous calls, outcomes of previous calls and length of time between calls. We discuss how these differences can be used to increase the likelihood of contacting cooperative respondents and completing interviews for both sample types.

The molecular responses of skeletal muscle satellite cells to continuous expression of IGF-1: implications for the rescue of induced muscular atrophy in aged rats

NASA Technical Reports Server (NTRS)

Chakravarthy, M. V.; Booth, F. W.; Spangenburg, E. E.

2001-01-01

Approximately 50% of humans older than 85 years have physical frailty due to weak skeletal muscles. This indicates a need for determining mechanisms to combat this problem. A critical cellular factor for postnatal muscle growth is a population of myogenic precursor cells called satellite cells. Given the complex process of sarcopenia, it has been postulated that, at some point in this process, a limited satellite cell proliferation potential could become rate-limiting to the regrowth of old muscles. It is conceivable that if satellite cell proliferative capacity can be maintained or enhanced with advanced age, sarcopenia could potentially be delayed or prevented. Therefore, the purposes of this paper are to describe whether IGF-I can prevent muscular atrophy induced by repeated cycles of hindlimb immobilization, increase the in vitro proliferation in satellite cells from these muscles and, if so, the molecular mechanisms by which IGF-I mediates this increased proliferation. Our results provide evidence that IGF-I can enhance aged muscle regrowth possibly through increased satellite cell proliferation. The results also suggest that IGF-I enhances satellite cell proliferation by decreasing the cell cycle inhibitor, p27Kip1, through the PI3'-K/Akt pathway. These data provide molecular evidence for IGF-I's rescue effect upon aging-associated skeletal muscle atrophy.

Solar power satellites - Heat engine or solar cells

NASA Technical Reports Server (NTRS)

Oman, H.; Gregory, D. L.

1978-01-01

A solar power satellite is the energy-converting element of a system that can deliver some 10 GW of power to utilities on the earth's surface. We evaluated heat engines and solar cells for converting sunshine to electric power at the satellite. A potassium Rankine cycle was the best of the heat engines, and 50 microns thick single-crystal silicon cells were the best of the photovoltaic converters. Neither solar cells nor heat engines had a clear advantage when all factors were considered. The potassium-turbine power plant, however, was more difficult to assemble and required a more expensive orbital assembly base. We therefore based our cost analyses on solar-cell energy conversion, concluding that satellite-generated power could be delivered to utilities for around 4 to 5 cents a kWh.

Muscle Satellite Cells: Exploring the Basic Biology to Rule Them.

PubMed

Almeida, Camila F; Fernandes, Stephanie A; Ribeiro Junior, Antonio F; Keith Okamoto, Oswaldo; Vainzof, Mariz

2016-01-01

Adult skeletal muscle is a postmitotic tissue with an enormous capacity to regenerate upon injury. This is accomplished by resident stem cells, named satellite cells, which were identified more than 50 years ago. Since their discovery, many researchers have been concentrating efforts to answer questions about their origin and role in muscle development, the way they contribute to muscle regeneration, and their potential to cell-based therapies. Satellite cells are maintained in a quiescent state and upon requirement are activated, proliferating, and fusing with other cells to form or repair myofibers. In addition, they are able to self-renew and replenish the stem pool. Every phase of satellite cell activity is highly regulated and orchestrated by many molecules and signaling pathways; the elucidation of players and mechanisms involved in satellite cell biology is of extreme importance, being the first step to expose the crucial points that could be modulated to extract the optimal response from these cells in therapeutic strategies. Here, we review the basic aspects about satellite cells biology and briefly discuss recent findings about therapeutic attempts, trying to raise questions about how basic biology could provide a solid scaffold to more successful use of these cells in clinics.

Myostatin signals through Pax7 to regulate satellite cell self-renewal

DOE Office of Scientific and Technical Information (OSTI.GOV)

McFarlane, Craig; Department of Biological Sciences, University of Waikato, Hamilton; Hennebry, Alex

2008-01-15

Myostatin, a Transforming Growth Factor-beta (TGF-{beta}) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-dependent mechanism to regulate satellite cell self-renewal. While excess Myostatin inhibited Pax7 expression via ERK1/2 signaling, an increase in Pax7 expression was observed following both genetic inactivation and functional antagonism of Myostatin. As a result, we show that either blocking or inactivating Myostatin enhances the partitioning of the fusion-incompetent self-renewed satellite cell lineage (high Pax7 expression, lowmore » MyoD expression) from the pool of actively proliferating myogenic precursor cells. Consistent with this result, over-expression of Pax7 in C2C12 myogenic cells resulted in increased self-renewal through a mechanism which slowed both myogenic proliferation and differentiation. Taken together, these results suggest that increased expression of Pax7 promotes satellite cell self-renewal, and furthermore Myostatin may control the process of satellite cell self-renewal through regulation of Pax7. Thus we speculate that, in addition to the intrinsic factors (such as Pax7), extrinsic factors both positive and negative in nature, will play a major role in determining the stemness of skeletal muscle satellite cells.« less

Transduction of satellite cells after prenatal intramuscular administration of lentiviral vectors.

PubMed

MacKenzie, Tippi C; Kobinger, Gary P; Louboutin, Jean-Pierre; Radu, Antoneta; Javazon, Elizabeth H; Sena-Esteves, Miguel; Wilson, James M; Flake, Alan W

2005-01-01

We have previously reported long-term expression of lacZ in myocytes after in utero intramuscular injection of Mokola and Ebola pseudotyped lentiviral vectors. In further experiments, we have noted that these vectors also transduce small cells at the periphery of the muscle fibers that have the morphology of satellite cells, or muscle stem cells. In this study we performed experiments to further define the morphology and function of these cells. Balb/c mice at 14-15 days gestation were injected intramuscularly with Ebola or Mokola pseudotyped lentiviral vectors carrying CMV-lacZ. Animals were harvested at various time points, muscles were stained with X-gal, and processed for electron microscopy (EM) and immunofluorescence. To determine whether transduced satellite cells were functionally capable of regenerating injured muscles, animals were injected with notexin in the same area 8 weeks after the in utero injection of viral vector. Transmission EM of transduced cells confirmed the ultrastructural appearance of satellite cells. Double immunofluorescence for beta-galactosidase and satellite cell markers demonstrated co-localization of these markers in transduced cells. In the notexin-injured animals, small blue cells were seen at the areas of regeneration that co-localized beta-galactosidase with markers of regenerating satellite cells. Central nucleated blue fibers were seen at late time points, indicating regenerated muscle fibers arising from a transduced satellite cell. This study demonstrates transduction of muscle satellite cells following prenatal viral vector mediated gene transfer. These findings may have important implications for gene therapy strategies directed toward muscular dystrophy.

BRE facilitates skeletal muscle regeneration by promoting satellite cell motility and differentiation

PubMed Central

Xiao, Lihai; Lee, Kenneth Ka Ho

2016-01-01

ABSTRACT The function of the Bre gene in satellite cells was investigated during skeletal muscle regeneration. The tibialis anterior leg muscle was experimentally injured in Bre knockout mutant (BRE-KO) mice. It was established that the accompanying muscle regeneration was impaired as compared with their normal wild-type counterparts (BRE-WT). There were significantly fewer pax7+ satellite cells and smaller newly formed myofibers present in the injury sites of BRE-KO mice. Bre was required for satellite cell fusion and myofiber formation. The cell fusion index and average length of newly-formed BRE-KO myofibers were found to be significantly reduced as compared with BRE-WT myofibers. It is well established that satellite cells are highly invasive which confers on them the homing ability to reach the muscle injury sites. Hence, we tracked the migratory behavior of these cells using time-lapse microscopy. Image analysis revealed no difference in directionality of movement between BRE-KO and BRE-WT satellite cells but there was a significant decrease in the velocity of BRE-KO cell movement. Moreover, chemotactic migration assays indicated that BRE-KO satellite cells were significantly less responsive to chemoattractant SDF-1α than BRE-WT satellite cells. We also established that BRE normally protects CXCR4 from SDF-1α-induced degradation. In sum, BRE facilitates skeletal muscle regeneration by enhancing satellite cell motility, homing and fusion. PMID:26740569

The Satellite Cell in Male and Female, Developing and Adult Mouse Muscle: Distinct Stem Cells for Growth and Regeneration

PubMed Central

Neal, Alice; Boldrin, Luisa; Morgan, Jennifer Elizabeth

2012-01-01

Satellite cells are myogenic cells found between the basal lamina and the sarcolemma of the muscle fibre. Satellite cells are the source of new myofibres; as such, satellite cell transplantation holds promise as a treatment for muscular dystrophies. We have investigated age and sex differences between mouse satellite cells in vitro and assessed the importance of these factors as mediators of donor cell engraftment in an in vivo model of satellite cell transplantation. We found that satellite cell numbers are increased in growing compared to adult and in male compared to female adult mice. We saw no difference in the expression of the myogenic regulatory factors between male and female mice, but distinct profiles were observed according to developmental stage. We show that, in contrast to adult mice, the majority of satellite cells from two week old mice are proliferating to facilitate myofibre growth; however a small proportion of these cells are quiescent and not contributing to this growth programme. Despite observed changes in satellite cell populations, there is no difference in engraftment efficiency either between satellite cells derived from adult or pre-weaned donor mice, male or female donor cells, or between male and female host muscle environments. We suggest there exist two distinct satellite cell populations: one for muscle growth and maintenance and one for muscle regeneration. PMID:22662253

Cellular dynamics in the muscle satellite cell niche

PubMed Central

Bentzinger, C Florian; Wang, Yu Xin; Dumont, Nicolas A; Rudnicki, Michael A

2013-01-01

Satellite cells, the quintessential skeletal muscle stem cells, reside in a specialized local environment whose anatomy changes dynamically during tissue regeneration. The plasticity of this niche is attributable to regulation by the stem cells themselves and to a multitude of functionally diverse cell types. In particular, immune cells, fibrogenic cells, vessel-associated cells and committed and differentiated cells of the myogenic lineage have emerged as important constituents of the satellite cell niche. Here, we discuss the cellular dynamics during muscle regeneration and how disease can lead to perturbation of these mechanisms. To define the role of cellular components in the muscle stem cell niche is imperative for the development of cell-based therapies, as well as to better understand the pathobiology of degenerative conditions of the skeletal musculature. PMID:24232182

Differential expression of FGF receptors and of myogenic regulatory factors in primary cultures of satellite cells originating from fast (EDL) and slow (Soleus) twitch rat muscles.

PubMed

Martelly, I; Soulet, L; Bonnavaud, S; Cebrian, J; Gautron, J; Barritault, D

2000-11-01

In the rat, the fast and slow twitch muscles respectively Extensor digitorum longus (EDL) and Soleus present differential characteristics during regeneration. This suggests that their satellite cells responsible for muscle growth and repair represent distinct cellular populations. We have previously shown that satellite cells dissociated from Soleus and grown in vitro proliferate more readily than those isolated from EDL muscle. Fibroblast growth factors (FGFs) are known as regulators of myoblast proliferation and several studies have revealed a relationship between the response of myoblasts to FGF and the expression of myogenic regulatory factors (MRF) of the MyoD family by myoblasts. Therefore, we presently examined the possibility that the satellite cells isolated from EDL and Soleus muscles differ in the expression of FGF receptors (FGF-R) and of MRF expression. FGF-R1 and -R4 were strongly expressed in proliferating cultures whereas FGF-R2 and R3 were not detected in these cultures. In differentiating cultures, only -R1 was present in EDL satellite cells while FGF-R4 was also still expressed in Soleus cells. Interestingly, the unconventional receptor for FGF called cystein rich FGF receptor (CFR), of yet unknown function, was mainly detected in EDL satellite cell cultures. Soleus and EDL satellite cell cultures also differed in the expression MRFs. These results are consistent with the notion that satellite cells from fast and slow twitch muscles belong to different types of myogenic cells and suggest that satellite cells might play distinct roles in the formation and diversification of fast and slow fibres.

FGF2 activates TRPC and Ca2+ signaling leading to satellite cell activation

PubMed Central

Liu, Yewei; Schneider, Martin F.

2013-01-01

Satellite cells, as stem cells of adult skeletal muscle, are tightly associated with the differentiated muscle fibers and remain quiescent in the absence of muscle damage. In response to an injury, the quiescent satellite cell is activated by soluble factors, including FGFs released from injured myofibers. Using immunostaining, we here first show that TRPC1 channels are highly expressed in satellite cells attached to muscle fibers. Since CD34, a traditional stem cell marker, was recently found to be expressed in skeletal muscle satellite cells we labeled living satellite cells in their physiological niche associated with host FDB fibers using anti-CD34-FITC antibody. We then monitored intra-cellular calcium in anti-CD34-FITC labeled satellite cells attached to muscle fibers using the calcium sensitive dye X rhod-1 which has little fluorescence cross talk with FITC. FGF2 increased intracellular calcium in satellite cells, which was antagonized by the TRPC channel blocker SKF 96365. Immunostaining showed that NFATc3 is highly expressed in satellite cells, but not in host FDB fibers. Elevation of intracellular calcium by FGF2 is accompanied by nuclear translocation of NFATc3 and NFATc2 and by an increase in the number of MyoD positive cells per muscle fiber, both of which were attenuated by TRPC blocker SKF 96365. Our results suggest a novel pathway of satellite cell activation where FGF2 enhances calcium influx through a TRPC channel, and the increased cytosolic calcium leads to both NFATc3 and NFATc2 nuclear translocation and enhanced number of MyoD positive satellite cells per muscle fiber. PMID:24575047

Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy

PubMed Central

Fry, Christopher S.; Lee, Jonah D.; Jackson, Janna R.; Kirby, Tyler J.; Stasko, Shawn A.; Liu, Honglu; Dupont-Versteegden, Esther E.; McCarthy, John J.; Peterson, Charlotte A.

2014-01-01

Our aim in the current study was to determine the necessity of satellite cells for long-term muscle growth and maintenance. We utilized a transgenic Pax7-DTA mouse model, allowing for the conditional depletion of > 90% of satellite cells with tamoxifen treatment. Synergist ablation surgery, where removal of synergist muscles places functional overload on the plantaris, was used to stimulate robust hypertrophy. Following 8 wk of overload, satellite cell-depleted muscle demonstrated an accumulation of extracellular matrix (ECM) and fibroblast expansion that resulted in reduced specific force of the plantaris. Although the early growth response was normal, an attenuation of hypertrophy measured by both muscle wet weight and fiber cross-sectional area occurred in satellite cell-depleted muscle. Isolated primary myogenic progenitor cells (MPCs) negatively regulated fibroblast ECM mRNA expression in vitro, suggesting a novel role for activated satellite cells/MPCs in muscle adaptation. These results provide evidence that satellite cells regulate the muscle environment during growth.—Fry, C. S., Lee, J. D., Jackson, J. R., Kirby, T. J., Stasko, S. A., Liu, H., Dupont-Versteegden, E. E., McCarthy, J. J., Peterson, C. A. Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy. PMID:24376025

SIRT1 synchs satellite cell metabolism with stem cell fate.

PubMed

Diaz-Ruiz, Alberto; Gonzalez-Freire, Marta; Ferrucci, Luigi; Bernier, Michel; de Cabo, Rafael

2015-02-05

Metabolic reprogramming of muscle stem cells modulates myogenic cell fate. In this issue of Cell Stem Cell, Ryall et al. (2015) show that SIRT1, a NAD(+)-dependent histone deacetylase, acts as an epigenetic regulator that connects changes in satellite cell metabolism with changes in the transcriptional machinery toward myogenic commitment. Copyright © 2015 Elsevier Inc. All rights reserved.

Satellite cell depletion prevents fiber hypertrophy in skeletal muscle.

PubMed

Egner, Ingrid M; Bruusgaard, Jo C; Gundersen, Kristian

2016-08-15

The largest mammalian cells are the muscle fibers, and they have multiple nuclei to support their large cytoplasmic volumes. During hypertrophic growth, new myonuclei are recruited from satellite stem cells into the fiber syncytia, but it was recently suggested that such recruitment is not obligatory: overload hypertrophy after synergist ablation of the plantaris muscle appeared normal in transgenic mice in which most of the satellite cells were abolished. When we essentially repeated these experiments analyzing the muscles by immunohistochemistry and in vivo and ex vivo imaging, we found that overload hypertrophy was prevented in the satellite cell-deficient mice, in both the plantaris and the extensor digitorum longus muscles. We attribute the previous findings to a reliance on muscle mass as a proxy for fiber hypertrophy, and to the inclusion of a significant number of regenerating fibers in the analysis. We discuss that there is currently no model in which functional, sustainable hypertrophy has been unequivocally demonstrated in the absence of satellite cells; an exception is re-growth, which can occur using previously recruited myonuclei without addition of new myonuclei. © 2016. Published by The Company of Biologists Ltd.

Loss of niche-satellite cell interactions in syndecan-3 null mice alters muscle progenitor cell homeostasis improving muscle regeneration.

PubMed

Pisconti, Addolorata; Banks, Glen B; Babaeijandaghi, Farshad; Betta, Nicole Dalla; Rossi, Fabio M V; Chamberlain, Jeffrey S; Olwin, Bradley B

2016-01-01

The skeletal muscle stem cell niche provides an environment that maintains quiescent satellite cells, required for skeletal muscle homeostasis and regeneration. Syndecan-3, a transmembrane proteoglycan expressed in satellite cells, supports communication with the niche, providing cell interactions and signals to maintain quiescent satellite cells. Syndecan-3 ablation unexpectedly improves regeneration in repeatedly injured muscle and in dystrophic mice, accompanied by the persistence of sublaminar and interstitial, proliferating myoblasts. Additionally, muscle aging is improved in syndecan-3 null mice. Since syndecan-3 null myofiber-associated satellite cells downregulate Pax7 and migrate away from the niche more readily than wild type cells, syxndecan-3 appears to regulate satellite cell homeostasis and satellite cell homing to the niche. Manipulating syndecan-3 provides a promising target for development of therapies to enhance muscle regeneration in muscular dystrophies and in aged muscle.

Karyopherin alpha 1 regulates satellite cell proliferation and survival by modulating nuclear import

PubMed Central

Choo, Hyo-Jung; Cutler, Alicia; Rother, Franziska; Bader, Michael; Pavlath, Grace K.

2016-01-01

Satellite cells are stem cells with an essential role in skeletal muscle repair. Precise regulation of gene expression is critical for proper satellite cell quiescence, proliferation, differentiation and self -renewal. Nuclear proteins required for gene expression are dependent on the nucleocytoplasmic transport machinery to access to nucleus, however little is known about regulation of nuclear transport in satellite cells. The best characterized nuclear import pathway is classical nuclear import which depends on a classical nuclear localization signal (cNLS) in a cargo protein and the heterodimeric import receptors, karyopherin alpha (KPNA) and beta (KPNB). Multiple KPNA1 paralogs exist and can differ in importing specific cNLS proteins required for cell differentiation and function. We show that transcripts for six Kpna paralogs underwent distinct changes in mouse satellite cells during muscle regeneration accompanied by changes in cNLS proteins in nuclei. Depletion of KPNA1, the most dramatically altered KPNA, caused satellite cells in uninjured muscle to prematurely activate, proliferate and undergo apoptosis leading to satellite cell exhaustion with age. Increased proliferation of satellite cells led to enhanced muscle regeneration at early stages of regeneration. In addition, we observed impaired nuclear localization of two key KPNA1 cargo proteins: p27, a cyclin-dependent kinase inhibitor associated with cell cycle control and lymphoid enhancer factor 1, a critical co-transcription factor for β-catenin. These results indicate that regulated nuclear import of proteins by KPNA1 is critical for satellite cell proliferation and survival and establish classical nuclear import as a novel regulatory mechanism for controlling satellite cell fate. PMID:27434733

[Satellite glial cells in sensory ganglia: its role in pain].

PubMed

Costa, Filipa Alexandra Leite; Moreira Neto, Fani Lourença

2015-01-01

Satellite glial cells in sensory ganglia are a recent subject of research in the field of pain and a possible therapeutic target in the future. Therefore, the aim of this study was to summarize some of the important physiological and morphological characteristics of these cells and gather the most relevant scientific evidence about its possible role in the development of chronic pain. In the sensory ganglia, each neuronal body is surrounded by satellite glial cells forming distinct functional units. This close relationship enables bidirectional communication via a paracrine signaling between those two cell types. There is a growing body of evidence that glial satellite cells undergo structural and biochemical changes after nerve injury, which influence neuronal excitability and consequently the development and/or maintenance of pain in different animal models of chronic pain. Satellite glial cells are important in the establishment of physiological pain, in addition to being a potential target for the development of new pain treatments. Copyright © 2014 Sociedade Brasileira de Anestesiologia. Publicado por Elsevier Editora Ltda. All rights reserved.

Myostatin Suppression of Akirin1 Mediates Glucocorticoid-Induced Satellite Cell Dysfunction

PubMed Central

Dong, Yanjun; Pan, Jenny S.; Zhang, Liping

2013-01-01

Glucocorticoids production is increased in many pathological conditions that are associated with muscle loss, but their role in causing muscle wasting is not fully understood. We have demonstrated a new mechanism of glucocorticoid-induced muscle atrophy: Dexamethasone (Dex) suppresses satellite cell function contributing to the development of muscle atrophy. Specifically, we found that Dex decreases satellite cell proliferation and differentiation in vitro and in vivo. The mechanism involved Dex-induced upregulation of myostatin and suppression of Akirin1, a promyogenic gene. When myostatin was inhibited in Dex-treated mice, Akirin1 expression increased as did satellite cell activity, muscle regeneration and muscle growth. In addition, silencing myostatin in myoblasts or satellite cells prevented Dex from suppressing Akirin1 expression and cellular proliferation and differentiation. Finally, overexpression of Akirin1 in myoblasts increased their expression of MyoD and myogenin and improved cellular proliferation and differentiation, theses improvements were no longer suppressed by Dex. We conclude that glucocorticoids stimulate myostatin which inhibits Akirin1 expression and the reparative functions of satellite cells. These responses attribute to muscle atrophy. Thus, inhibition of myostatin or increasing Akirin1 expression could lead to therapeutic strategies for improving satellite cell activation and enhancing muscle growth in diseases associated with increased glucocorticoid production. PMID:23516508

Myostatin suppression of Akirin1 mediates glucocorticoid-induced satellite cell dysfunction.

PubMed

Dong, Yanjun; Pan, Jenny S; Zhang, Liping

2013-01-01

Glucocorticoids production is increased in many pathological conditions that are associated with muscle loss, but their role in causing muscle wasting is not fully understood. We have demonstrated a new mechanism of glucocorticoid-induced muscle atrophy: Dexamethasone (Dex) suppresses satellite cell function contributing to the development of muscle atrophy. Specifically, we found that Dex decreases satellite cell proliferation and differentiation in vitro and in vivo. The mechanism involved Dex-induced upregulation of myostatin and suppression of Akirin1, a promyogenic gene. When myostatin was inhibited in Dex-treated mice, Akirin1 expression increased as did satellite cell activity, muscle regeneration and muscle growth. In addition, silencing myostatin in myoblasts or satellite cells prevented Dex from suppressing Akirin1 expression and cellular proliferation and differentiation. Finally, overexpression of Akirin1 in myoblasts increased their expression of MyoD and myogenin and improved cellular proliferation and differentiation, theses improvements were no longer suppressed by Dex. We conclude that glucocorticoids stimulate myostatin which inhibits Akirin1 expression and the reparative functions of satellite cells. These responses attribute to muscle atrophy. Thus, inhibition of myostatin or increasing Akirin1 expression could lead to therapeutic strategies for improving satellite cell activation and enhancing muscle growth in diseases associated with increased glucocorticoid production.

A myogenic precursor cell that could contribute to regeneration in zebrafish and its similarity to the satellite cell.

PubMed

Siegel, Ashley L; Gurevich, David B; Currie, Peter D

2013-09-01

The cellular basis for mammalian muscle regeneration has been an area of intense investigation over recent decades. The consensus is that a specialized self-renewing stem cell, termed the satellite cell, plays a major role during the process of regeneration in amniotes. How broadly this mechanism is deployed within the vertebrate phylogeny remains an open question. A lack of information on the role of cells analogous to the satellite cell in other vertebrate systems is even more unexpected given the fact that satellite cells were first designated in frogs. An intriguing aspect of this debate is that a number of amphibia and many fish species exhibit epimorphic regenerative processes in specific tissues, whereby regeneration occurs by the dedifferentiation of the damaged tissue, without deploying specialized stem cell populations analogous to satellite cells. Hence, it is feasible that a cellular process completely distinct from that deployed during mammalian muscle regeneration could operate in species capable of epimorphic regeneration. In this minireview, we examine the evidence for the broad phylogenetic distribution of satellite cells. We conclude that, in the vertebrates examined so far, epimorphosis does not appear to be deployed during muscle regeneration, and that analogous cells expressing similar marker genes to satellite cells appear to be deployed during the regenerative process. However, the functional definition of these cells as self-renewing muscle stem cells remains a final hurdle to the definition of the satellite cell as a generic vertebrate cell type. © 2013 FEBS.

Comparison and analysis of Wuding and avian chicken skeletal muscle satellite cells.

PubMed

Tong, H Q; Jiang, Z Q; Dou, T F; Li, Q H; Xu, Z Q; Liu, L X; Gu, D H; Rong, H; Huang, Y; Chen, X B; Jois, M; Te Pas, M F W; Ge, C R; Jia, J J

2016-10-05

Chicken skeletal muscle satellite cells are located between the basement membrane and the sarcolemma of mature muscle fibers. Avian broilers have been genetically selected based on their high growth velocity and large muscle mass. The Wuding chicken is a famous local chicken in Yunnan Province that undergoes non-selection breeding and is slow growing. In this study, we aimed to explore differences in the proliferation and differentiation properties of satellite cells isolated from the two chicken breeds. Using immunofluorescence, hematoxylin-eosin staining and real-time polymerase chain reaction analysis, we analyzed the in vitro characteristics of proliferating and differentiating satellite cells isolated from the two chicken breeds. The growth curve of satellite cells was S-shaped, and cells from Wuding chickens entered the logarithmic phase and plateau phase 1 day later than those from Avian chicken. The results also showed that the two skeletal muscle satellite cell lines were positive for Pax7, MyoD and IGF-1. The expression of Pax7 followed a downward trend, whereas that of MyoD and IGF-1 first increased and subsequently decreased in cells isolated from the two chickens. These data indicated that the skeletal muscle satellite cells of Avian chicken grow and differentiate faster than did those of Wuding chickens. We suggest that the methods of breeding selection applied to these breeds regulate the characteristics of skeletal muscle satellite cells to influence muscle growth.

Satellite Conferences

Science.gov Websites

NOAA- NESDIS Banner Satellite Conferences Collage images of earth, POES and GOES satellites in space HOME Call for Poster Abstracts DOC Logo NOAA Logo Satellite Conferences Welcome to the website for National Oceanic and Atmospheric Administration (NOAA) Satellite Conferences; past, present and future

Skeletal Muscle Satellite Cell Activation Following Cutaneous Burn in Rats

DTIC Science & Technology

2013-12-01

satellite cell activation and survival during oxidative stress. J Muscle Res Cell Motil 2011;32(2):99–109. [33] Rathbone CR, Booth FW, Lees SJ. Sirt1 ...Skeletal muscle satellite cell activation following cutaneous burn in rats Xiaowu Wu*, Thomas J. Walters, Christopher R. Rathbone Extremity Trauma...f o Article history: Accepted 15 October 2012 Keywords: Muscle precursor cell Thermal injury Atrophy Skeletal muscle Activation a b s t r a c t

Myostatin inhibition induces muscle fibre hypertrophy prior to satellite cell activation.

PubMed

Wang, Qian; McPherron, Alexandra C

2012-05-01

Muscle fibres are multinucleated post-mitotic cells that can change dramatically in size during adulthood. It has been debated whether muscle fibre hypertrophy requires activation and fusion of muscle stem cells, the satellite cells. Myostatin (MSTN) is a negative regulator of skeletal muscle growth during development and in the adult, and MSTN inhibition is therefore a potential therapy for muscle wasting diseases, some of which are associated with a depletion of satellite cells. Conflicting results have been obtained in previous analyses of the role of MSTN on satellite cell quiescence. Here, we inhibited MSTN in adult mice with a soluble activin receptor type IIB and analysed the incorporation of new nuclei using 5-bromo-2-deoxyuridine (BrdU) labelling by isolating individual myofibres. We found that satellite cells are activated by MSTN inhibition. By varying the dose and time course for MSTN inhibition, however, we found that myofibre hypertrophy precedes the incorporation of new nuclei, and that the overall number of new nuclei is relatively low compared to the number of total myonuclei. These results reconcile some of the previous work obtained by other methods. In contrast with previous reports, we also found that Mstn null mice do not have increased satellite cell numbers during adulthood and are not resistant to sarcopaenia. Our results support a previously proposed model of hypertrophy in which hypertrophy can precede satellite cell activation. Studies of the metabolic and functional effects of postnatal MSTN inhibition are needed to determine the consequences of increasing the cytoplasm/myonuclear ratio after MSTN inhibition.

Myostatin inhibits proliferation of human urethral rhabdosphincter satellite cells.

PubMed

Akita, Yasuyuki; Sumino, Yasuhiro; Mori, Ken-ichi; Nomura, Takeo; Sato, Fuminori; Mimata, Hiromitsu

2013-05-01

Myostatin, a member of the transforming growth factor-β superfamily, is a negative regulator of myogenesis in skeletal muscle. We examined the effect of myostatin and myostatin inhibition by an antagonistic agent, follistatin, on growth of human urethral rhabdosphincter satellite cells (muscle stem cells) to develop a new strategy for treatment of stress urinary incontinence. Rhabdosphincter satellite cells were cultured and selected by magnetic affinity cell sorting using an anti-neural cell adhesion molecule antibody. The cells were transfected with simian virus-40 antigen to extend their lifespan. A cell proliferation assay, a cell cycle analysis and an investigation of signal transduction were carried out. The autocrine action of endogenous myostatin by western blotting, real-time reverse transcription polymerase chain reaction and immunoneutralization using an anti-myostatin antibody was also evaluated. Selectively cultured cells expressed markers of striated muscles and successfully differentiated into myotubes. Myostatin inhibited proliferation of these cells through Smad2 phosphorylation and cell cycle arrest. Inhibitory effects of myostatin were reversed by addition of follistatin. However, rhabdosphincter satellite cells did not appear to use autocrine secretion of myostatin to regulate their proliferation. Inhibition of myostatin function might be a useful pathway in the development of novel strategies for stimulating rhabdosphincter cells regeneration to treat stress urinary incontinence. © 2012 The Japanese Urological Association.

AMP-activated Protein Kinase Stimulates Warburg-like Glycolysis and Activation of Satellite Cells during Muscle Regeneration*

PubMed Central

Fu, Xing; Zhu, Mei-Jun; Dodson, Mike V.; Du, Min

2015-01-01

Satellite cells are the major myogenic stem cells residing inside skeletal muscle and are indispensable for muscle regeneration. Satellite cells remain largely quiescent but are rapidly activated in response to muscle injury, and the derived myogenic cells then fuse to repair damaged muscle fibers or form new muscle fibers. However, mechanisms eliciting metabolic activation, an inseparable step for satellite cell activation following muscle injury, have not been defined. We found that a noncanonical Sonic Hedgehog (Shh) pathway is rapidly activated in response to muscle injury, which activates AMPK and induces a Warburg-like glycolysis in satellite cells. AMPKα1 is the dominant AMPKα isoform expressed in satellite cells, and AMPKα1 deficiency in satellite cells impairs their activation and myogenic differentiation during muscle regeneration. Drugs activating noncanonical Shh promote proliferation of satellite cells, which is abolished because of satellite cell-specific AMPKα1 knock-out. Taken together, AMPKα1 is a critical mediator linking noncanonical Shh pathway to Warburg-like glycolysis in satellite cells, which is required for satellite activation and muscle regeneration. PMID:26370082

Effects of voluntary wheel running on satellite cells in the rat plantaris muscle.

PubMed

Kurosaka, Mitsutoshi; Naito, Hisashi; Ogura, Yuji; Kojima, Atsushi; Goto, Katsumasa; Katamoto, Shizuo

2009-01-01

This study investigated the effects of voluntary wheel running on satellite cells in the rat plantaris muscle. Seventeen 5-week-old male Wistar rats were assigned to a control (n = 5) or training (n = 12) group. Each rat in the training group ran voluntarily in a running-wheel cage for 8 weeks. After the training period, the animals were anesthetized, and the plantaris muscles were removed, weighed, and analyzed immunohistochemically and biochemically. Although there were no significant differences in muscle weight or fiber area between the groups, the numbers of satellite cells and myonuclei per muscle fiber, percentage of satellite cells, and citrate synthase activity were significantly higher in the training group compared with the control group (p < 0.05). The percentage of satellite cells was also positively correlated with distance run in the training group (r = 0.61, p < 0.05). Voluntary running can induce an increase in the number of satellite cells without changing the mean fiber area in the rat plantaris muscle; this increase in satellite cell content is a function of distance run. Key pointsThere is no study about the effect of voluntary running on satellite cells in the rat plantaris muscle.Voluntary running training causes an increase of citrate synthase activity in the rat plantaris muscle but does not affect muscle weight and mean fiber area in the rat plantaris muscle.Voluntary running can induce an increase in the number of satellite cells without hypertrophy of the rat plantaris muscle.

TRAF6 regulates satellite stem cell self-renewal and function during regenerative myogenesis

PubMed Central

Hindi, Sajedah M.; Kumar, Ashok

2015-01-01

Satellite cells are a stem cell population within adult muscle and are responsible for myofiber regeneration upon injury. Satellite cell dysfunction has been shown to underlie the loss of skeletal muscle mass in many acquired and genetic muscle disorders. The transcription factor paired box-protein-7 (PAX7) is indispensable for supplementing the reservoir of satellite cells and driving regeneration in normal and diseased muscle. TNF receptor–associated factor 6 (TRAF6) is an adaptor protein and an E3 ubiquitin ligase that mediates the activation of multiple cell signaling pathways in a context-dependent manner. Here, we demonstrated that TRAF6-mediated signaling is critical for homeostasis of satellite cells and their function during regenerative myogenesis. Selective deletion of Traf6 in satellite cells of adult mice led to profound muscle regeneration defects and dramatically reduced levels of PAX7 and late myogenesis markers. TRAF6 was required for the activation of MAPKs ERK1/2 and JNK1/2, which in turn activated the transcription factor c-JUN, which binds the Pax7 promoter and augments Pax7 expression. Moreover, TRAF6/c-JUN signaling repressed the levels of the microRNAs miR-1 and miR-206, which promote differentiation, to maintain PAX7 levels in satellite cells. We also determined that satellite cell–specific deletion of Traf6 exaggerates the dystrophic phenotype in the mdx (a mouse model of Duchenne muscular dystrophy) mouse by blunting the regeneration of injured myofibers. Collectively, our study reveals an essential role for TRAF6 in satellite stem cell function. PMID:26619121

SVEP1 is a novel marker of activated pre-determined skeletal muscle satellite cells.

PubMed

Shefer, Gabi; Benayahu, Dafna

2010-03-01

In this study we explored the expression pattern of SVEP1, a novel cell adhesion molecule (CAM), in bona fide satellite cells and their immediate progeny. We show that SVEP1 is expressed in activated satellite cells prior to their determination to the myogenic lineage. SVEP1 was also expressed during early phases of myogenic differentiation through the initial stage of myoblast fusion and myotube formation. The expression of SVEP1 was shown by immunostaining two cell culture systems: freshly isolated myofibers and primary myoblasts. Pax7 was used to pinpoint satellite cells situated in their niche on myofibers, and activated satellite cells were determined based on BrdU incorporation (Pax7(+)/BrdU(+)cells). MyoD marked satellite cells fated to undergo myogenesis as well as proliferating and differentiating myoblasts. Differentiating myoblasts and myotubes were identified based on their sarcomeric myosin expression. We showed that SVEP1 was specifically expressed in pre-determined activated satellite cells (Pax7(+)/ BrdU(+) /MyoD(-)) accounting for about 24% of total satellite cells. On the other hand, SVEP1 expression was absent in quiescent satellite cells (Pax7(+)/BrdU(-)/MyoD(-)). Moreover, based on MyoD/sarcomeric myosin co-expression SVEP1 was shown to be expressed throughout the early phases of myogenesis up until myoblast fusion and myotube formation. A decline in SVEP1 expression occurred upon myotube maturation. We suggest SVEP1 as a potential biomarker for pre-fated satellite cells. The impact of this finding is that it may allow scrutinizing signals that affect differentiation commitment. Thus, holds a therapeutic potential for maladies that involve deregulated stem cell fate-decision.

The number of satellite cells in slow and fast fibres from human vastus lateralis muscle.

PubMed

Kadi, Fawzi; Charifi, Nadia; Henriksson, Jan

2006-07-01

The aim of this investigation was to study the distribution of satellite cells in slow (type I fibres) and fast (type II fibres) fibres from human vastus lateralis muscle. This muscle is characterised by a mixed fibre type composition and is considered as the site of choice for biopsies in research work and for clinical diagnosis. Biopsy samples were obtained from five healthy young volunteers and a total of 1,747 type I fibres and 1,760 type II fibres were assessed. Satellite cells and fibre type composition were studied on serial muscle cross-sections stained with specific monoclonal antibodies. From a total of 218 satellite cells, 116 satellite cells were found in contact with type I fibres (53.6+/-8% of the satellite cells associated to type I fibres) and 102 satellite cells in contact with type II fibres (46.4+/-8% of the satellite cells associated to type II fibres). There was no significant difference (P=0.4) between the percentages of satellite cells in contact with type I and with type II fibres. Additionally, there was no relationship between the mean number of satellite cells per fibre and the mean cross-sectional area of muscle fibres. In conclusion, our results show that there is no fibre type-specific distribution of satellite cells in a human skeletal muscle with mixed fibre type composition.

Skeletal Muscle Satellite Cells Are Committed to Myogenesis and Do Not Spontaneously Adopt Nonmyogenic Fates

PubMed Central

Starkey, Jessica D.; Yamamoto, Masakazu; Yamamoto, Shoko; Goldhamer, David J.

2011-01-01

The developmental potential of skeletal muscle stem cells (satellite cells) remains controversial. The authors investigated satellite cell developmental potential in single fiber and clonal cultures derived from MyoDiCre/+;R26REYFP/+ muscle, in which essentially all satellite cells are permanently labeled. Approximately 60% of the clones derived from cells that co-purified with muscle fibers spontaneously underwent adipogenic differentiation. These adipocytes stained with Oil-Red-O and expressed the terminal differentiation markers, adipsin and fatty acid binding protein 4, but did not express EYFP and were therefore not of satellite cell origin. Satellite cells mutant for either MyoD or Myf-5 also maintained myogenic programming in culture and did not adopt an adipogenic fate. Incorporation of additional wash steps prior to muscle fiber plating virtually eliminated the non-myogenic cells but did not reduce the number of adherent Pax7+ satellite cells. More than half of the adipocytes observed in cultures from Tie2-Cre mice were recombined, further demonstrating a non-satellite cell origin. Under adipogenesis-inducing conditions, satellite cells accumulated cytoplasmic lipid but maintained myogenic protein expression and did not fully execute the adipogenic differentiation program, distinguishing them from adipocytes observed in muscle fiber cultures. The authors conclude that skeletal muscle satellite cells are committed to myogenesis and do not spontaneously adopt an adipogenic fate. PMID:21339173

Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells.

PubMed

Tosic, Milica; Allen, Anita; Willmann, Dominica; Lepper, Christoph; Kim, Johnny; Duteil, Delphine; Schüle, Roland

2018-01-25

Satellite cells are muscle stem cells required for muscle regeneration upon damage. Of note, satellite cells are bipotent and have the capacity to differentiate not only into skeletal myocytes, but also into brown adipocytes. Epigenetic mechanisms regulating fate decision and differentiation of satellite cells during muscle regeneration are not yet fully understood. Here, we show that elevated levels of lysine-specific demethylase 1 (Kdm1a, also known as Lsd1) have a beneficial effect on muscle regeneration and recovery after injury, since Lsd1 directly regulates key myogenic transcription factor genes. Importantly, selective Lsd1 ablation or inhibition in Pax7-positive satellite cells, not only delays muscle regeneration, but changes cell fate towards brown adipocytes. Lsd1 prevents brown adipocyte differentiation of satellite cells by repressing expression of the novel pro-adipogenic transcription factor Glis1. Together, downregulation of Glis1 and upregulation of the muscle-specific transcription program ensure physiological muscle regeneration.

Cell Phone Calls in the Operating Theater and Staff Distractions: An Observational Study.

PubMed

Avidan, Alexander; Yacobi, Galel; Weissman, Charles; Levin, Phillip D

2017-01-09

Cell phones are the primary communication tool in our institution. There are no restrictions on their use in the operating rooms. The goal of this study was to evaluate the extent of cell phone use in the operating rooms during elective surgery and to evaluate whether they cause staff distractions. The following data on cell phone use were recorded anonymously: number of incoming and outgoing cell phone calls, duration of cell phone calls and their content (patient related, work related, private), who was distracted by the cell phone calls, and duration of distractions. We made observations during 52 surgeries. There were 205 cell phone calls, 197 (96.1%; median, 3 per surgery; interquartile range, 2-5) incoming and 8 (3.9%) outgoing. Incoming calls were answered on 110 (55.8%) of 197 (median, 2; interquartile range, 1-3) occasions. The mean duration of incoming calls (64 ± 40 seconds) was shorter than those of the outgoing calls (137 ± 242 seconds, P < 0.001). During 29 (14.7%) of 197 incoming calls, 30 staff distractions occurred. Distractions were caused mainly for surgeons talking on their cell phones (24/30, 80.0%). The mean duration of the distractions was 43.6 ± 22.3 seconds. During all 8 outgoing calls, no other staff members were distracted. The number of cell phone calls in the operating rooms during elective surgery was lower than expected and caused short-lived distractions mainly to the operating surgeons. We recommend that operating surgeons turn off their cell phones before surgery.

47 CFR 25.284 - Emergency Call Center Service.

Code of Federal Regulations, 2010 CFR

2010-10-01

... mobile satellite service to end-user customers (part 25, subparts A-D) must provide Emergency Call Center... Center personnel must determine the emergency caller's phone number and location and then transfer or otherwise redirect the call to an appropriate public safety answering point. Providers of mobile satellite...

Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development.

PubMed

Mitchell, Kathryn J; Pannérec, Alice; Cadot, Bruno; Parlakian, Ara; Besson, Vanessa; Gomes, Edgar R; Marazzi, Giovanna; Sassoon, David A

2010-03-01

Satellite cells are resident myogenic progenitors in postnatal skeletal muscle involved in muscle postnatal growth and adult regenerative capacity. Here, we identify and describe a population of muscle-resident stem cells, which are located in the interstitium, that express the cell stress mediator PW1 but do not express other markers of muscle stem cells such as Pax7. PW1(+)/Pax7(-) interstitial cells (PICs) are myogenic in vitro and efficiently contribute to skeletal muscle regeneration in vivo as well as generating satellite cells and PICs. Whereas Pax7 mutant satellite cells show robust myogenic potential, Pax7 mutant PICs are unable to participate in myogenesis and accumulate during postnatal growth. Furthermore, we found that PICs are not derived from a satellite cell lineage. Taken together, our findings uncover a new and anatomically identifiable population of muscle progenitors and define a key role for Pax7 in a non-satellite cell population during postnatal muscle growth.

Invited Review: Extrinsic regulation of domestic animal-derived myogenic satellite cells II

USDA-ARS?s Scientific Manuscript database

The existence of myogenic satellite cells was reported some forty-seven years ago, and, since that time, satellite cell research has flourished. So much new information is generated (daily) on these cells that it can be difficult for individuals to keep abreast of important issues related to the act...

A Pitx2-MicroRNA Pathway Modulates Cell Proliferation in Myoblasts and Skeletal-Muscle Satellite Cells and Promotes Their Commitment to a Myogenic Cell Fate

PubMed Central

Lozano-Velasco, Estefanía; Vallejo, Daniel; Esteban, Francisco J.; Doherty, Chris; Hernández-Torres, Francisco; Franco, Diego

2015-01-01

The acquisition of a proliferating-cell status from a quiescent state as well as the shift between proliferation and differentiation are key developmental steps in skeletal-muscle stem cells (satellite cells) to provide proper muscle regeneration. However, how satellite cell proliferation is regulated is not fully understood. Here, we report that the c-isoform of the transcription factor Pitx2 increases cell proliferation in myoblasts by downregulating microRNA 15b (miR-15b), miR-23b, miR-106b, and miR-503. This Pitx2c-microRNA (miRNA) pathway also regulates cell proliferation in early-activated satellite cells, enhancing Myf5+ satellite cells and thereby promoting their commitment to a myogenic cell fate. This study reveals unknown functions of several miRNAs in myoblast and satellite cell behavior and thus may have future applications in regenerative medicine. PMID:26055324

mTOR is necessary for proper satellite cell activity and skeletal muscle regeneration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Pengpeng; Department of Animal Sciences, Purdue University, West Lafayette, IN 47907; Liang, Xinrong

The serine/threonine kinase mammalian target of rapamycin (mTOR) is a key regulator of protein synthesis, cell proliferation and energy metabolism. As constitutive deletion of Mtor gene results in embryonic lethality, the function of mTOR in muscle stem cells (satellite cells) and skeletal muscle regeneration remains to be determined. In this study, we established a satellite cell specific Mtor conditional knockout (cKO) mouse model by crossing Pax7{sup CreER} and Mtor{sup flox/flox} mice. Skeletal muscle regeneration after injury was severely compromised in the absence of Mtor, indicated by increased number of necrotic myofibers infiltrated by Evans blue dye, and reduced number andmore » size of regenerated myofibers in the Mtor cKO mice compared to wild type (WT) littermates. To dissect the cellular mechanism, we analyzed satellite cell-derived primary myoblasts grown on single myofibers or adhered to culture plates. The Mtor cKO myoblasts exhibited defective proliferation and differentiation kinetics when compared to myoblasts derived from WT littermates. At the mRNA and protein levels, the Mtor cKO myoblasts expressed lower levels of key myogenic determinant genes Pax7, Myf5, Myod, Myog than did the WT myoblasts. These results suggest that mTOR is essential for satellite cell function and skeletal muscle regeneration through controlling the expression of myogenic genes. - Highlights: • Pax7{sup CreER} was used to delete Mtor gene in satellite cells. • Satellite cell specific deletion of Mtor impairs muscle regeneration. • mTOR is necessary for satellite cell proliferation and differentiation. • Deletion of Mtor leads to reduced expression of key myogenic genes.« less

Differential requirement for satellite cells during overload-induced muscle hypertrophy in growing versus mature mice.

PubMed

Murach, Kevin A; White, Sarah H; Wen, Yuan; Ho, Angel; Dupont-Versteegden, Esther E; McCarthy, John J; Peterson, Charlotte A

2017-07-10

Pax7+ satellite cells are required for skeletal muscle fiber growth during post-natal development in mice. Satellite cell-mediated myonuclear accretion also appears to persist into early adulthood. Given the important role of satellite cells during muscle development, we hypothesized that the necessity of satellite cells for adaptation to an imposed hypertrophic stimulus depends on maturational age. Pax7 CreER -R26R DTA mice were treated for 5 days with vehicle (satellite cell-replete, SC+) or tamoxifen (satellite cell-depleted, SC-) at 2 months (young) and 4 months (mature) of age. Following a 2-week washout, mice were subjected to sham surgery or 10 day synergist ablation overload of the plantaris (n = 6-9 per group). The surgical approach minimized regeneration, de novo fiber formation, and fiber splitting while promoting muscle fiber growth. Satellite cell density (Pax7+ cells/fiber), embryonic myosin heavy chain expression (eMyHC), and muscle fiber cross sectional area (CSA) were evaluated via immunohistochemistry. Myonuclei (myonuclei/100 mm) were counted on isolated single muscle fibers. Tamoxifen treatment depleted satellite cells by ≥90% and prevented myonuclear accretion with overload in young and mature mice (p < 0.05). Satellite cells did not recover in SC- mice after overload. Average muscle fiber CSA increased ~20% in young SC+ (p = 0.07), mature SC+ (p < 0.05), and mature SC- mice (p < 0.05). In contrast, muscle fiber hypertrophy was prevented in young SC- mice. Muscle fiber number increased only in mature mice after overload (p < 0.05), and eMyHC expression was variable, specifically in mature SC+ mice. Reliance on satellite cells for overload-induced hypertrophy is dependent on maturational age, and global responses to overload differ in young versus mature mice.

Fusogenic micropeptide Myomixer is essential for satellite cell fusion and muscle regeneration.

PubMed

Bi, Pengpeng; McAnally, John R; Shelton, John M; Sánchez-Ortiz, Efrain; Bassel-Duby, Rhonda; Olson, Eric N

2018-04-10

Regeneration of skeletal muscle in response to injury occurs through fusion of a population of stem cells, known as satellite cells, with injured myofibers. Myomixer, a muscle-specific membrane micropeptide, cooperates with the transmembrane protein Myomaker to regulate embryonic myoblast fusion and muscle formation. To investigate the role of Myomixer in muscle regeneration, we used CRISPR/Cas9-mediated genome editing to generate conditional knockout Myomixer alleles in mice. We show that genetic deletion of Myomixer in satellite cells using a tamoxifen-regulated Cre recombinase transgene under control of the Pax7 promoter abolishes satellite cell fusion and prevents muscle regeneration, resulting in severe muscle degeneration after injury. Satellite cells devoid of Myomixer maintain expression of Myomaker, demonstrating that Myomaker alone is insufficient to drive myoblast fusion. These findings, together with prior studies demonstrating the essentiality of Myomaker for muscle regeneration, highlight the obligatory partnership of Myomixer and Myomaker for myofiber formation throughout embryogenesis and adulthood.

Satellite-like cells contribute to pax7-dependent skeletal muscle repair in adult zebrafish

PubMed Central

Berberoglu, Michael A.; Gallagher, Thomas L.; Morrow, Zachary T.; Talbot, Jared C.; Hromowyk, Kimberly J.; Tenente, Inês M.; Langenau, David M.; Amacher, Sharon L.

2017-01-01

Satellite cells, also known as muscle stem cells, are responsible for skeletal muscle growth and repair in mammals. Pax7 and Pax3 transcription factors are established satellite cell markers required for muscle development and regeneration, and there is great interest in identifying additional factors that regulate satellite cell proliferation, differentiation, and/or skeletal muscle regeneration. Due to the powerful regenerative capacity of many zebrafish tissues, even in adults, we are exploring the regenerative potential of adult zebrafish skeletal muscle. Here, we show that adult zebrafish skeletal muscle contains cells similar to mammalian satellite cells. Adult zebrafish satellite-like cells have dense heterochromatin, express Pax7 and Pax3, proliferate in response to injury, and show peak myogenic responses 4–5 days post-injury (dpi). Furthermore, using a pax7a-driven GFP reporter, we present evidence implicating satellite-like cells as a possible source of new muscle. In lieu of central nucleation, which distinguishes regenerating myofibers in mammals, we describe several characteristics that robustly identify newly-forming myofibers from surrounding fibers in injured adult zebrafish muscle. These characteristics include partially overlapping expression in satellite cells and regenerating myofibers of two RNA-binding proteins Rbfox2 and Rbfoxl1, known to regulate embryonic muscle development and function. Finally, by analyzing pax7a; pax7b double mutant zebrafish, we show that Pax7 is required for adult skeletal muscle repair, as it is in the mouse. PMID:28279710

The effect of nutritional status and myogenic satellite cell age on turkey satellite cell proliferation, differentiation, and expression of myogenic transcriptional regulatory factors and heparan sulfate proteoglycans syndecan-4 and glypican-1.

PubMed

Harthan, Laura B; McFarland, Douglas C; Velleman, Sandra G

2014-01-01

Posthatch satellite cell mitotic activity is a critical component of muscle development and growth. Satellite cells are myogenic stem cells that can be induced by nutrition to follow other cellular developmental pathways, and whose mitotic activity declines with age. The objective of the current study was to determine the effect of restricting protein synthesis on the proliferation and differentiation, expression of myogenic transcriptional regulatory factors myogenic determination factor 1, myogenin, and myogenic regulatory factor 4, and expression of the heparan sulfate proteoglycans syndecan-4 and glypican-1 in satellite cells isolated from 1-d-, 7-wk-, and 16-wk-old turkey pectoralis major muscle (1 d, 7 wk, and 16 wk cells, respectively) by using variable concentrations of Met and Cys. Four Met concentrations-30 (control), 7.5, 3, or 0 mg/L with 3.2 mg/L of Cys per 1 mg/L of Met-were used for culture of satellite cells to determine the effect of nutrition and age on satellite cell behavior during proliferation and differentiation. Proliferation was reduced by lower Met and Cys concentrations in all ages at 96 h of proliferation. Differentiation was increased in the 1 d Met-restricted cells, whereas the 7 wk cells treated with 3 mg/L of Met had decreased differentiation. Reduced Met and Cys levels from the control did not significantly affect the 16 wk cells at 72 h of differentiation. However, medium with no Met or Cys suppressed differentiation at all ages. The expression of myogenic determination factor 1, myogenin, myogenic regulatory factor 4, syndecan-4, and glypican-1 was differentially affected by age and Met or Cys treatment. These data demonstrate the age-specific manner in which turkey pectoralis major muscle satellite cells respond to nutritional availability and the importance of defining optimal nutrition to maximize satellite cell proliferation and differentiation for subsequent muscle mass accretion.

Akt1 deficiency diminishes skeletal muscle hypertrophy by reducing satellite cell proliferation.

PubMed

Moriya, Nobuki; Miyazaki, Mitsunori

2018-05-01

Skeletal muscle mass is determined by the net dynamic balance between protein synthesis and degradation. Although the Akt/mechanistic target of rapamycin (mTOR)-dependent pathway plays an important role in promoting protein synthesis and subsequent skeletal muscle hypertrophy, the precise molecular regulation of mTOR activity by the upstream protein kinase Akt is largely unknown. In addition, the activation of satellite cells has been indicated as a key regulator of muscle mass. However, the requirement of satellite cells for load-induced skeletal muscle hypertrophy is still under intense debate. In this study, female germline Akt1 knockout (KO) mice were used to examine whether Akt1 deficiency attenuates load-induced skeletal muscle hypertrophy through suppressing mTOR-dependent signaling and satellite cell proliferation. Akt1 KO mice showed a blunted hypertrophic response of skeletal muscle, with a diminished rate of satellite cell proliferation following mechanical overload. In contrast, Akt1 deficiency did not affect the load-induced activation of mTOR signaling and the subsequent enhanced rate of protein synthesis in skeletal muscle. These observations suggest that the load-induced activation of mTOR signaling occurs independently of Akt1 regulation and that Akt1 plays a critical role in regulating satellite cell proliferation during load-induced muscle hypertrophy.

The PERK arm of the unfolded protein response regulates satellite cell-mediated skeletal muscle regeneration

PubMed Central

Xiong, Guangyan; Hindi, Sajedah M; Mann, Aman K; Gallot, Yann S; Bohnert, Kyle R; Cavener, Douglas R; Whittemore, Scott R; Kumar, Ashok

2017-01-01

Regeneration of skeletal muscle in adults is mediated by satellite stem cells. Accumulation of misfolded proteins triggers endoplasmic reticulum stress that leads to unfolded protein response (UPR). The UPR is relayed to the cell through the activation of PERK, IRE1/XBP1, and ATF6. Here, we demonstrate that levels of PERK and IRE1 are increased in satellite cells upon muscle injury. Inhibition of PERK, but not the IRE1 arm of the UPR in satellite cells inhibits myofiber regeneration in adult mice. PERK is essential for the survival and differentiation of activated satellite cells into the myogenic lineage. Deletion of PERK causes hyper-activation of p38 MAPK during myogenesis. Blocking p38 MAPK activity improves the survival and differentiation of PERK-deficient satellite cells in vitro and muscle formation in vivo. Collectively, our results suggest that the PERK arm of the UPR plays a pivotal role in the regulation of satellite cell homeostasis during regenerative myogenesis. DOI: http://dx.doi.org/10.7554/eLife.22871.001 PMID:28332979

Label-Free, High-Throughput Purification of Satellite Cells Using Microfluidic Inertial Separation.

PubMed

Syverud, Brian C; Lin, Eric; Nagrath, Sunitha; Larkin, Lisa M

2018-01-01

Skeletal muscle satellite cells have tremendous therapeutic potential in cell therapy or skeletal muscle tissue engineering. Obtaining a sufficiently pure satellite cell population, however, presents a significant challenge. We hypothesized that size differences between satellite cells and fibroblasts, two primary cell types obtained from skeletal muscle dissociation, would allow for label-free, inertial separation in a microfluidic device, termed a "Labyrinth," and that these purified satellite cells could be used to engineer skeletal muscle. Throughout tissue fabrication, Labyrinth-purified cells were compared with unsorted controls to assess the efficiency of this novel sorting process and to examine potential improvements in myogenic proliferation, differentiation, and tissue function. Immediately after dissociation and Labyrinth sorting, cells were immunostained to identify myogenic cells and fibroblast progenitors. Remaining cells were cultured for 14 days to form a confluent monolayer that was induced to delaminate and was captured as a 3D skeletal muscle construct. During monolayer development, myogenic proliferation (BrdU assay on Day 4), differentiation and myotube fusion index (α-actinin on Day 11), and myotube structural development (light microscopy on Day 14) were assessed. Isometric tetanic force production was measured in 3D constructs on Day 16. Immediately following sorting, unsorted cells exhibited a myogenic purity of 39.9% ± 3.99%, and this purity was enriched approximately two-fold to 75.5% ± 1.59% by microfluidic separation. The BrdU assay on Day 4 similarly showed significantly enhanced myogenic proliferation: in unsorted controls 47.0% ± 2.77% of proliferating cells were myogenic, in comparison to 61.7% ± 2.55% following purification. Myogenic differentiation and fusion, assessed by fusion index quantification, showed improvement from 82.7% ± 3.74% in control to 92.3% ± 2.04% in the purified cell population

Depletion of Pax7+ satellite cells does not affect diaphragm adaptations to running in young or aged mice.

PubMed

Murach, Kevin A; Confides, Amy L; Ho, Angel; Jackson, Janna R; Ghazala, Lina S; Peterson, Charlotte A; Dupont-Versteegden, Esther E

2017-10-01

Satellite cell depletion does not affect diaphragm adaptations to voluntary wheel running in young or aged mice. Satellite cell depletion early in life (4 months of age) has minimal effect on diaphragm phenotype by old age (24 months). Prolonged satellite cell depletion in the diaphragm does not result in excessive extracellular matrix accumulation, in contrast to what has been reported in hind limb muscles. Up-regulation of Pax3 mRNA+ cells after satellite cell depletion in young and aged mice suggests that Pax3+ cells may compensate for a loss of Pax7+ satellite cells in the diaphragm. Future investigations should focus on the role of Pax3+ cells in the diaphragm during adaptation to exercise and ageing. Satellite cell contribution to unstressed diaphragm is higher compared to hind limb muscles, which is probably attributable to constant activation of this muscle to drive ventilation. Whether satellite cell depletion negatively impacts diaphragm quantitative and qualitative characteristics under stressed conditions in young and aged mice is unknown. We therefore challenged the diaphragm with prolonged running activity in the presence and absence of Pax7+ satellite cells in young and aged mice using an inducible Pax7 CreER -R26R DTA model. Mice were vehicle (Veh, satellite cell-replete) or tamoxifen (Tam, satellite cell-depleted) treated at 4 months of age and were then allowed to run voluntarily at 6 months (young) and 22 months (aged). Age-matched, cage-dwelling, Veh- and Tam-treated mice without wheel access served as activity controls. Diaphragm muscles were analysed from young (8 months) and aged (24 months) mice. Satellite cell depletion did not alter diaphragm mean fibre cross-sectional area, fibre type distribution or extracellular matrix content in young or aged mice, regardless of running activity. Resting in vivo diaphragm function was also unaffected by satellite cell depletion. Myonuclear density was maintained in young satellite cell

Moderate-intensity treadmill running promotes expansion of the satellite cell pool in young and old mice.

PubMed

Shefer, Gabi; Rauner, Gat; Stuelsatz, Pascal; Benayahu, Dafna; Yablonka-Reuveni, Zipora

2013-09-01

Satellite cells, the myogenic progenitors located at the myofibre surface, are essential for the repair of adult skeletal muscle. There is ample evidence for an age-linked decline in the number of satellite cells and performance in limb muscles. Hence, an effective means of activating and expanding the satellite cell pool may enhance muscle maintenance and reduce the impact of age-associated muscle deterioration (sarcopaenia). Accordingly, in the present study, we explored the beneficial effects of endurance exercise on satellite cells in young and old mice. Animals were subjected to an 8-week moderate-intensity treadmill-running approach that does not inflict apparent muscle damage (0° inclination, 11.5 m·min(-1) for 30 min·day(-1) , 6 days·week(-1) ). Myofibres of extensor digitorum longus muscles were then isolated from exercised and sedentary mice and used for monitoring the number of satellite cells, as well as for harvesting individual satellite cells for clonal growth assays. We specifically focused on satellite cell pools of single myofibres, with the view that daily wear of muscles probably affects individual myofibres rather than causing overall muscle damage. We found an expansion of the satellite cell pool in the exercised groups compared to the sedentary groups, with the same increase (~ 1.6-fold) in both ages. The results of the present study are in agreement with our findings obtained using rat gastrocnemius, indicating the consistent effect of exercise on satellite cell expansion in limb muscles. The experimental paradigm established in the present study is useful for investigating satellite cell dynamics at the myofibre niche, as well as for broader investigations of the impact of physiologically and pathologically relevant factors on adult myogenesis. © 2013 The Authors Journal compilation © 2013 FEBS.

The effect of exercise mode on the acute response of satellite cells in old men.

PubMed

Nederveen, J P; Joanisse, S; Séguin, C M L; Bell, K E; Baker, S K; Phillips, S M; Parise, G

2015-12-01

A dysregulation of satellite cells may contribute to the progressive loss of muscle mass that occurs with age; however, older adults retain the ability to activate and expand their satellite cell pool in response to exercise. The modality of exercise capable of inducing the greatest acute response is unknown. We sought to characterize the acute satellite cell response following different modes of exercise in older adults. Sedentary older men (n = 22; 67 ± 4 years; 27 ± 2.6 kg*m(-2) ) were randomly assigned to complete an acute bout of either resistance exercise, high-intensity interval exercise on a cycle ergometer or moderate-intensity aerobic exercise. Muscle biopsies were obtained before, 24 and 48 h following each exercise bout. The satellite cell response was analysed using immunofluorescent microscopy of muscle cross sections. Satellite cell expansion associated with type I fibres was observed 24 and 48 h following resistance exercise only (P ˂ 0.05), while no expansion of type II-associated satellite cells was observed in any group. There was a greater number of activated satellite cells 24 h following resistance exercise (pre: 1.3 ± 0.1, 24 h: 4.8 ± 0.5 Pax7 + /MyoD+cells/100 fibres) and high-intensity interval exercise (pre: 0.7 ± 0.3, 24 h: 3.1 ± 0.3 Pax7 + /MyoD+cells/100 fibres) (P ˂ 0.05). The percentage of type I-associated SC co-expressing MSTN was reduced only in the RE group 24 h following exercise (pre: 87 ± 4, 24 h: 57 ± 5%MSTN+ type I SC) (P < 0.001). Although resistance exercise is the most potent exercise type to induce satellite cell pool expansion, high-intensity interval exercise was also more potent than moderate-intensity aerobic exercise in inducing satellite cell activity. © 2015 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

Identification and functional characterization of muscle satellite cells in Drosophila

PubMed Central

Reichert, Heinrich

2017-01-01

Work on genetic model systems such as Drosophila and mouse has shown that the fundamental mechanisms of myogenesis are remarkably similar in vertebrates and invertebrates. Strikingly, however, satellite cells, the adult muscle stem cells that are essential for the regeneration of damaged muscles in vertebrates, have not been reported in invertebrates. In this study, we show that lineal descendants of muscle stem cells are present in adult muscle of Drosophila as small, unfused cells observed at the surface and in close proximity to the mature muscle fibers. Normally quiescent, following muscle fiber injury, we show that these cells express Zfh1 and engage in Notch-Delta-dependent proliferative activity and generate lineal descendant populations, which fuse with the injured muscle fiber. In view of strikingly similar morphological and functional features, we consider these novel cells to be the Drosophila equivalent of vertebrate muscle satellite cells. PMID:29072161

MASTR directs MyoD-dependent satellite cell differentiation during skeletal muscle regeneration

PubMed Central

Mokalled, Mayssa H.; Johnson, Aaron N.; Creemers, Esther E.; Olson, Eric N.

2012-01-01

In response to skeletal muscle injury, satellite cells, which function as a myogenic stem cell population, become activated, expand through proliferation, and ultimately fuse with each other and with damaged myofibers to promote muscle regeneration. Here, we show that members of the Myocardin family of transcriptional coactivators, MASTR and MRTF-A, are up-regulated in satellite cells in response to skeletal muscle injury and muscular dystrophy. Global and satellite cell-specific deletion of MASTR in mice impairs skeletal muscle regeneration. This impairment is substantially greater when MRTF-A is also deleted and is due to aberrant differentiation and excessive proliferation of satellite cells. These abnormalities mimic those associated with genetic deletion of MyoD, a master regulator of myogenesis, which is down-regulated in the absence of MASTR and MRTF-A. Consistent with an essential role of MASTR in transcriptional regulation of MyoD expression, MASTR activates a muscle-specific postnatal MyoD enhancer through associations with MEF2 and members of the Myocardin family. Our results provide new insights into the genetic circuitry of muscle regeneration and identify MASTR as a central regulator of this process. PMID:22279050

Coordination of Satellite Cell Activation and Self-Renewal by Par-Complex-Dependent Asymmetric Activation of p38α/β MAPK

PubMed Central

Troy, Andrew; Cadwallader, Adam B.; Fedorov, Yuri; Tyner, Kristina; Tanaka, Kathleen Kelly; Olwin, Bradley B.

2014-01-01

SUMMARY In response to muscle injury, satellite cells activate the p38α/β MAPK pathway to exit quiescence, then proliferate, repair skeletal muscle, and self-renew, replenishing the quiescent satellite cell pool. Although satellite cells are capable of asymmetric division, the mechanisms regulating satellite cell self-renewal are not understood. We found that satellite cells, once activated, enter the cell cycle and a subset undergoes asymmetric division, renewing the satellite cell pool. Asymmetric localization of the Par complex activates p38α/β MAPK in only one daughter cell, inducing MyoD, which permits cell cycle entry and generates a proliferating myoblast. The absence of p38α/β MAPK signaling in the other daughter cell prevents MyoD induction, renewing the quiescent satellite cell. Thus, satellite cells employ a mechanism to generate distinct daughter cells, coupling the Par complex and p38α/β MAPK signaling to link the response to muscle injury with satellite cell self-renewal. PMID:23040480

Next Generation Lithium-Ion Cell For Satellite Applications

NASA Astrophysics Data System (ADS)

Inoue, Takefumi; Segawa, Masazumi; Yoshida, Hiroaki; Takeda, Koichi

2011-10-01

GS Yuasa Technology has standard line up cells for satellite applications since 1999. The design of these cells is not changed and their production will continue. GS Yuasa is now developing higher performance Next Generation Lithium-ion cells. These cells have an improved positive material, negative material, electrolyte, and separator and have demonstrated excellent capacity retention with very low DC resistance growth during life testing. The new cell has approximately 40% more EOL energy (Wh/kg), and slightly lighter weight while using the same size components as our existing products.

Acoustic satellite behaviour in the Australian bushcricket Elephantodeta nobilis (Phaneropterinae, Tettigoniidae, Orthoptera).

PubMed

Bailey; Field

2000-02-01

Male and female Elephantodeta nobilis duet with the female responding to the male's long and complex call. The duetting male's call consisted of four parts, described here as parts A, B, C and D. We found that the female replied 570 ms after the male's D pulse, which followed the extended part B and short click of part C. Noncalling males were attracted to the duet and often used satellite tactics by inserting a volley of clicks 200 ms before the alpha male's D pulse. Satellite males used part C of the alpha male song to cue their own call and this inserted call induced females to reply earlier compared with the alpha male call alone. Alpha males often extended their calls with additional D-type calls and so we examined the effectiveness of these calls as countermeasures to satellite calling. There was no influence of this alpha strategy on the satellite's propensity to call although more calls from the alpha male did cause the female to reply more frequently. We also examined the effect of relative intensity of alpha and satellite calls on the female's reply. Reduced satellite intensity increased the variance in the timing of the female response. Finally, we tested the effectiveness of the satellite's call on female phonotaxis within a two-speaker arena. Although females preferred the alpha male they were nevertheless attracted to the satellite calls regardless of the latter's relative intensity. We discuss the possible role of satellite calling as a novel conditional strategy. Copyright 2000 The Association for the Study of Animal Behaviour.

Characterisation of equine satellite cell transcriptomic profile response to β-hydroxy-β-methylbutyrate (HMB).

PubMed

Szcześniak, Katarzyna A; Ciecierska, Anna; Ostaszewski, Piotr; Sadkowski, Tomasz

2016-10-01

β-Hydroxy-β-methylbutyrate (HMB) is a popular ergogenic aid used by human athletes and as a supplement to sport horses, because of its ability to aid muscle recovery, improve performance and body composition. Recent findings suggest that HMB may stimulate satellite cells and affect expressions of genes regulating skeletal muscle cell growth. Despite the scientific data showing benefits of HMB supplementation in horses, no previous study has explained the mechanism of action of HMB in this species. The aim of this study was to reveal the molecular background of HMB action on equine skeletal muscle by investigating the transcriptomic profile changes induced by HMB in equine satellite cells in vitro. Upon isolation from the semitendinosus muscle, equine satellite cells were cultured until the 2nd day of differentiation. Differentiating cells were incubated with HMB for 24 h. Total cellular RNA was isolated, amplified, labelled and hybridised to microarray slides. Microarray data validation was performed with real-time quantitative PCR. HMB induced differential expressions of 361 genes. Functional analysis revealed that the main biological processes influenced by HMB in equine satellite cells were related to muscle organ development, protein metabolism, energy homoeostasis and lipid metabolism. In conclusion, this study demonstrated for the first time that HMB has the potential to influence equine satellite cells by controlling global gene expression. Genes and biological processes targeted by HMB in equine satellite cells may support HMB utility in improving growth and regeneration of equine skeletal muscle; however, the overall role of HMB in horses remains equivocal and requires further proteomic, biochemical and pharmacokinetic studies.

The Hippo pathway member Yap plays a key role in influencing fate decisions in muscle satellite cells

PubMed Central

Judson, Robert N.; Tremblay, Annie M.; Knopp, Paul; White, Robert B.; Urcia, Roby; De Bari, Cosimo; Zammit, Peter S.; Camargo, Fernando D.; Wackerhage, Henning

2012-01-01

Summary Satellite cells are the resident stem cells of skeletal muscle. Mitotically quiescent in mature muscle, they can be activated to proliferate and generate myoblasts to supply further myonuclei to hypertrophying or regenerating muscle fibres, or self-renew to maintain the resident stem cell pool. Here, we identify the transcriptional co-factor Yap as a novel regulator of satellite cell fate decisions. Yap expression increases during satellite cell activation and Yap remains highly expressed until after the differentiation versus self-renewal decision is made. Constitutive expression of Yap maintains Pax7+ and MyoD+ satellite cells and satellite cell-derived myoblasts, promotes proliferation but prevents differentiation. In contrast, Yap knockdown reduces the proliferation of satellite cell-derived myoblasts by ≈40%. Consistent with the cellular phenotype, microarrays show that Yap increases expression of genes associated with Yap inhibition, the cell cycle, ribosome biogenesis and that it represses several genes associated with angiotensin signalling. We also identify known regulators of satellite cell function such as BMP4, CD34 and Myf6 (Mrf4) as genes whose expression is dependent on Yap activity. Finally, we confirm in myoblasts that Yap binds to Tead transcription factors and co-activates MCAT elements which are enriched in the proximal promoters of Yap-responsive genes. PMID:23038772

A home away from home: challenges and opportunities in engineering in vitro muscle satellite cell niches

PubMed Central

Cosgrove, Benjamin D.; Sacco, Alessandra; Gilbert, Penney M.; Blau, Helen M.

2009-01-01

Satellite cells are skeletal muscle stem cells with a principal role in postnatal skeletal muscle regeneration. Satellite cells, like many tissue-specific adult stem cells, reside in a quiescent state in an instructive, anatomically defined niche. The satellite cell niche constitutes a distinct membrane-enclosed compartment within the muscle fiber, containing a diversity of biochemical and biophysical signals that influence satellite cell function. A major limitation to the study and clinical utility of satellite cells is that upon removal from the muscle fiber and plating in traditional plastic tissue culture platforms, their muscle stem cell properties are rapidly lost. Clearly, the maintenance of stem cell function is critically dependent on in vivo niche signals, highlighting the need to create novel in vitro microenvironments that allow for the maintenance and propagation of satellite cells while retaining their potential to function as muscle stem cells. Here, we discuss how emerging biomaterials technologies offer great promise for engineering in vitro microenvironments to meet these challenges. In engineered biomaterials, signaling molecules can be presented in a manner that more closely mimics cell-cell and cell-matrix interactions and matrices can be fabricated with diverse rigidities that approximate in vivo tissues. The development of in vitro microenvironments in which niche features can be systematically modulated will be instrumental not only to future insights into muscle stem cell biology and therapeutic approaches to muscle diseases and muscle wasting with aging, but also will provide a paradigm for the analysis of numerous adult tissue-specific stem cells. PMID:19751902

Alkaline regenerative fuel cell energy storage system for manned orbital satellites

NASA Technical Reports Server (NTRS)

Martin, R. E.; Gitlow, B.; Sheibley, D. W.

1982-01-01

It is pointed out that the alkaline regenerative fuel cell system represents a highly efficient, lightweight, reliable approach for providing energy storage in an orbiting satellite. In addition to its energy storage function, the system can supply hydrogen and oxygen for attitude control of the satellite and for life support. A summary is presented of the results to date obtained in connection with the NASA-sponsored fuel cell technology advancement program, giving particular attention to the requirements of the alkaline regenerative fuel cell and the low-earth mission. Attention is given to system design guidelines, weight considerations, gold-platinum cathode cell performance, matrix development, the electrolyte reservoir plate, and the cyclical load profile tests.

The effect of in vivo and in vitro irradiation (25 Gy) on the subsequent in vitro growth of satellite cells

NASA Technical Reports Server (NTRS)

Mozdziak, P. E.; Schultz, E.; Cassens, R. G.

1996-01-01

The effect of in vivo and in vitro irradiation on subsequent satellite cell growth, in vitro, was investigated to ascertain the ability of a 25 Gy dose to inhibit satellite cell proliferation. Satellite cells were isolated from the left (irradiated) and right (non-irradiated) Pectoralis thoracicus of two-week-old tom turkeys 16 h (n=3) and seven weeks (n=2) after the left Pectoralis thoracicus had been irradiated (25 Gy). Satellite cells isolated from the irradiated and non-irradiated muscles exhibited similar (P>0.10) in vitro proliferation indicating that a population of satellite cells survived an in vivo dose of 25 Gy. In additional experiments, satellite cell cultures derived from tom turkey Pectoralis thoracicus were irradiated (25 Gy) in vitro. The number of satellite cells did not (P>0.05) increase in irradiated cultures for 134 h following irradiation, while satellite cells in non-irradiated cultures proliferated (P<0.05) over this time. At later time periods, satellite cell number increased (P<0.05) in irradiated cultures indicating that a population of satellite cells survived irradiation. The results of these in vitro experiments suggest that a 25 Gy dose of irradiation does not abolish satellite cell divisions in the turkey Pectoralis thoracicus.

SOXF factors regulate murine satellite cell self-renewal and function through inhibition of β-catenin activity.

PubMed

Alonso-Martin, Sonia; Auradé, Frédéric; Mademtzoglou, Despoina; Rochat, Anne; Zammit, Peter S; Relaix, Frédéric

2018-06-08

Muscle satellite cells are the primary source of stem cells for postnatal skeletal muscle growth and regeneration. Understanding genetic control of satellite cell formation, maintenance, and acquisition of their stem cell properties is on-going, and we have identified SOXF (SOX7, SOX17, SOX18) transcriptional factors as being induced during satellite cell specification. We demonstrate that SOXF factors regulate satellite cell quiescence, self-renewal and differentiation. Moreover, ablation of Sox17 in the muscle lineage impairs postnatal muscle growth and regeneration. We further determine that activities of SOX7, SOX17 and SOX18 overlap during muscle regeneration, with SOXF transcriptional activity requisite. Finally, we show that SOXF factors also control satellite cell expansion and renewal by directly inhibiting the output of β-catenin activity, including inhibition of Ccnd1 and Axin2 . Together, our findings identify a key regulatory function of SoxF genes in muscle stem cells via direct transcriptional control and interaction with canonical Wnt/β-catenin signaling. © 2018, Alonso-Martin et al.

Label-free screening of niche-to-niche variation in satellite stem cells using functionalized pores

NASA Astrophysics Data System (ADS)

Chapman, Matthew R.; Balakrishnan, Karthik; Conboy, Michael J.; Mohanty, Swomitra; Jabart, Eric; Huang, Haiyan; Hack, James; Conboy, Irina M.; Sohn, Lydia L.

2012-02-01

Combinations of surface markers are currently used to identify muscle satellite cells. Using pores functionalized with specific antibodies and measuring the transit time of cells passing through these pores, we discovered remarkable heterogeneity in the expression of these markers in muscle (satellite) stem cells that reside in different single myofibers. Microniche-specific variation in stem cells of the same organ has not been previously described, as bulk analysis does not discriminate between separate myofibers or even separate hind-leg muscle groups. We found a significant population of Sca-1+ satellite cells that form myotubes, thereby demonstrating the myogenic potential of Sca-1+ cells, which are currently excluded in bulk sorting. Finally, using our label-free pore screening technique, we have been able to quantify directly surface expression of Notch1 without activation of the Notch pathway. We show for the first time Notch1-expression heterogeneity in unactivated satellite cells. The discovery of fiber-to-fiber variations prompts new research into the reasons for such diversity in muscle stem cells.

Inhibition of the Activin Receptor Type-2B Pathway Restores Regenerative Capacity in Satellite Cell-Depleted Skeletal Muscle.

PubMed

Formicola, Luigi; Pannérec, Alice; Correra, Rosa Maria; Gayraud-Morel, Barbara; Ollitrault, David; Besson, Vanessa; Tajbakhsh, Shahragim; Lachey, Jennifer; Seehra, Jasbir S; Marazzi, Giovanna; Sassoon, David A

2018-01-01

Degenerative myopathies typically display a decline in satellite cells coupled with a replacement of muscle fibers by fat and fibrosis. During this pathological remodeling, satellite cells are present at lower numbers and do not display a proper regenerative function. Whether a decline in satellite cells directly contributes to disease progression or is a secondary result is unknown. In order to dissect these processes, we used a genetic model to reduce the satellite cell population by ~70-80% which leads to a nearly complete loss of regenerative potential. We observe that while no overt tissue damage is observed following satellite cell depletion, muscle fibers atrophy accompanied by changes in the stem cell niche cellular composition. Treatment of these mice with an Activin receptor type-2B (AcvR2B) pathway blocker reverses muscle fiber atrophy as expected, but also restores regenerative potential of the remaining satellite cells. These findings demonstrate that in addition to controlling fiber size, the AcvR2B pathway acts to regulate the muscle stem cell niche providing a more favorable environment for muscle regeneration.

Inhibition of the Activin Receptor Type-2B Pathway Restores Regenerative Capacity in Satellite Cell-Depleted Skeletal Muscle

PubMed Central

Formicola, Luigi; Pannérec, Alice; Correra, Rosa Maria; Gayraud-Morel, Barbara; Ollitrault, David; Besson, Vanessa; Tajbakhsh, Shahragim; Lachey, Jennifer; Seehra, Jasbir S.; Marazzi, Giovanna; Sassoon, David A.

2018-01-01

Degenerative myopathies typically display a decline in satellite cells coupled with a replacement of muscle fibers by fat and fibrosis. During this pathological remodeling, satellite cells are present at lower numbers and do not display a proper regenerative function. Whether a decline in satellite cells directly contributes to disease progression or is a secondary result is unknown. In order to dissect these processes, we used a genetic model to reduce the satellite cell population by ~70–80% which leads to a nearly complete loss of regenerative potential. We observe that while no overt tissue damage is observed following satellite cell depletion, muscle fibers atrophy accompanied by changes in the stem cell niche cellular composition. Treatment of these mice with an Activin receptor type-2B (AcvR2B) pathway blocker reverses muscle fiber atrophy as expected, but also restores regenerative potential of the remaining satellite cells. These findings demonstrate that in addition to controlling fiber size, the AcvR2B pathway acts to regulate the muscle stem cell niche providing a more favorable environment for muscle regeneration. PMID:29881353

Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical gamma-oryzanol.

PubMed

Szcześniak, K A; Ciecierska, A; Ostaszewski, P; Sadkowski, T

2016-01-01

Adult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described. Equine satellite cells were isolated from semitendinosus muscle and cultured until the second day of differentiation. Differentiating cells were incubated with GO for the next 24 h. Subsequently, total RNA from GO-treated and control cells was isolated, amplified, labeled, and hybridized to two-color Horse Gene Expression Microarray slides. Quantitative PCR was used for the validation of microarray data. Our results revealed 58 genes with changed expression in GO-treated vs. control cells. Analysis of expression changes suggests that various processes are reinforced by GO in differentiating equine satellite cells, including inhibition of myoblast differentiation, increased proliferation and differentiation, stress response, and increased myogenic lineage commitment. The present study may confirm putative muscle-enhancing abilities of GO; however, the collective role of GO in skeletal myogenesis remains equivocal. The diversity of these changes is likely due to heterogenous growth rate of cells in primary culture. Genes identified in our study, modulated by the presence of GO, may become potential targets of future research investigating impact of this supplement in skeletal muscle on proteomic and biochemical level.

Creating a Software Framework for Simulating Satellite Geolocation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koch, Daniel B

2011-01-01

It is hard to imagine life these days without having some sort of electronic indication of one's current location. Whether the purpose is for business, personal, or emergency use, utilizing smart cell phones, in-vehicle navigation systems, or location beacons, dependence on the Global Positioning System (GPS) is pervasive. Yet the availability of the GPS should not be taken for granted. Both environmental (e.g., terrain, weather) and intentional interference (i.e., jamming) can reduce or deny satellite access. In order to investigate these and other issues, as well as to explore possible alternative satellite constellations, an application called the Satellite Simulation Toolkitmore » (SatSim) was created. This paper presents a high-level overview of SatSim and an example of how it may be used to study geolocation.« less

mTOR is necessary for proper satellite cell activity and skeletal muscle regeneration.

PubMed

Zhang, Pengpeng; Liang, Xinrong; Shan, Tizhong; Jiang, Qinyang; Deng, Changyan; Zheng, Rong; Kuang, Shihuan

The serine/threonine kinase mammalian target of rapamycin (mTOR) is a key regulator of protein synthesis, cell proliferation and energy metabolism. As constitutive deletion of Mtor gene results in embryonic lethality, the function of mTOR in muscle stem cells (satellite cells) and skeletal muscle regeneration remains to be determined. In this study, we established a satellite cell specific Mtor conditional knockout (cKO) mouse model by crossing Pax7(CreER) and Mtor(flox/flox) mice. Skeletal muscle regeneration after injury was severely compromised in the absence of Mtor, indicated by increased number of necrotic myofibers infiltrated by Evans blue dye, and reduced number and size of regenerated myofibers in the Mtor cKO mice compared to wild type (WT) littermates. To dissect the cellular mechanism, we analyzed satellite cell-derived primary myoblasts grown on single myofibers or adhered to culture plates. The Mtor cKO myoblasts exhibited defective proliferation and differentiation kinetics when compared to myoblasts derived from WT littermates. At the mRNA and protein levels, the Mtor cKO myoblasts expressed lower levels of key myogenic determinant genes Pax7, Myf5, Myod, Myog than did the WT myoblasts. These results suggest that mTOR is essential for satellite cell function and skeletal muscle regeneration through controlling the expression of myogenic genes. Copyright © 2015 Elsevier Inc. All rights reserved.

Participation of satellite glial cells of the dorsal root ganglia in acute nociception.

PubMed

Lemes, Júlia Borges Paes; de Campos Lima, Tais; Santos, Débora Oliveira; Neves, Amanda Ferreira; de Oliveira, Fernando Silva; Parada, Carlos Almicar; da Cruz Lotufo, Celina Monteiro

2018-05-29

At dorsal root ganglia, neurons and satellite glial cells (SGC) can communicate through ATP release and P2X7 receptor activation. SGCs are also interconnected by gap junctions and have been previously implicated in modulating inflammatory and chronic pain.We now present evidence that SGCs are also involved in processing acute nociception in rat dorsal root ganglia. Using primary dorsal root ganglia cultures we observed that calcium transients induced in neurons by capsaicin administration were followed by satellite glial cells activation. Only satellite glial cells response was reduced by administration of the P2X7 receptor antagonist A740003. In vivo, acute nociception induced by intraplantar injection of capsaicin in rats was inhibited by A740003 or by the gap junction blocker carbenoxolone administered at the dorsal root ganglia (L5 level). Both drugs also reduced the second phase of the formalin test. These results suggest that communication between neurons and satellite glial cells is not only involved in inflammatory or pathological pain, but also in the transmission of the nociceptive signal, possibly in situations involving C-fiber activation. Copyright © 2018 Elsevier B.V. All rights reserved.

Analytic studies on satellite detection of severe, two-cell tornadoes

NASA Technical Reports Server (NTRS)

Carrier, G. F.; Dergarabedian, P.; Fendell, F. E.

1979-01-01

It is argued that a two-cell structure is likely to be the unique property, and potentially satellite-accessible observable, of the exceptionally severe tornado. Analysis elucidating the dynamic, thermodynamic, and geometric properties of this two-cell structure is described. The analysis ultimately will furnish instrumentation requirements.

Retained differentiation capacity of human skeletal muscle satellite cells from spinal cord-injured individuals.

PubMed

Savikj, Mladen; Ruby, Maxwell A; Kostovski, Emil; Iversen, Per O; Zierath, Juleen R; Krook, Anna; Widegren, Ulrika

2018-06-01

Despite the well-known role of satellite cells in skeletal muscle plasticity, the effect of spinal cord injury on their function in humans remains unknown. We determined whether spinal cord injury affects the intrinsic ability of satellite cells to differentiate and produce metabolically healthy myotubes. We obtained vastus lateralis biopsies from eight spinal cord-injured and six able-bodied individuals. Satellite cells were isolated, grown and differentiated in vitro. Gene expression was measured by quantitative PCR. Abundance of differentiation markers and regulatory proteins was determined by Western blotting. Protein synthesis and fatty acid oxidation were measured by radioactive tracer-based assays. Activated satellite cells (myoblasts) and differentiated myotubes derived from skeletal muscle of able-bodied and spinal cord-injured individuals expressed similar (P > 0.05) mRNA levels of myogenic regulatory factors. Myogenic differentiation factor 1 expression was higher in myoblasts from spinal cord-injured individuals. Desmin and myogenin protein content was increased upon differentiation in both groups, while myotubes from spinal cord-injured individuals contained more type I and II myosin heavy chain. Phosphorylated and total protein levels of Akt-mechanistic target of rapamycin and forkhead box protein O signalling axes and protein synthesis rate in myotubes were similar (P > 0.05) between groups. Additionally, fatty acid oxidation of myotubes from spinal cord-injured individuals was unchanged (P > 0.05) compared to able-bodied controls. Our results indicate that the intrinsic differentiation capacity of satellite cells and metabolic characteristics of myotubes are preserved following spinal cord injury. This may inform potential interventions targeting satellite cell activation to alleviate skeletal muscle atrophy. © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American

Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise.

PubMed

Murach, Kevin A; Walton, R Grace; Fry, Christopher S; Michaelis, Sami L; Groshong, Jason S; Finlin, Brian S; Kern, Philip A; Peterson, Charlotte A

2016-09-01

This investigation evaluated whether moderate-intensity cycle ergometer training affects satellite cell and molecular responses to acute maximal concentric/eccentric resistance exercise in middle-aged women. Baseline and 72 h postresistance exercise vastus lateralis biopsies were obtained from seven healthy middle-aged women (56 ± 5 years, BMI 26 ± 1, VO2max 27 ± 4) before and after 12 weeks of cycle training. Myosin heavy chain (MyHC) I- and II-associated satellite cell density and cross-sectional area was determined via immunohistochemistry. Expression of 93 genes representative of the muscle-remodeling environment was also measured via NanoString. Overall fiber size increased ~20% with cycle training (P = 0.052). MyHC I satellite cell density increased 29% in response to acute resistance exercise before endurance training and 50% with endurance training (P < 0.05). Following endurance training, MyHC I satellite cell density decreased by 13% in response to acute resistance exercise (acute resistance × training interaction, P < 0.05). Genes with an interaction effect tracked with satellite cell behavior, increasing in the untrained state and decreasing in the endurance trained state in response to resistance exercise. Similar satellite cell and gene expression response patterns indicate coordinated regulation of the muscle environment to promote adaptation. Moderate-intensity endurance cycle training modulates the response to acute resistance exercise, potentially conditioning the muscle for more intense concentric/eccentric activity. These results suggest that cycle training is an effective endurance exercise modality for promoting growth in middle-aged women, who are susceptible to muscle mass loss with progressing age. © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

The effect of temperature on apoptosis and adipogenesis on skeletal muscle satellite cells derived from different muscle types

PubMed Central

Harding, Rachel L; Clark, Daniel L; Halevy, Orna; Coy, Cynthia S; Yahav, Shlomo; Velleman, Sandra G

2015-01-01

Satellite cells are multipotential stem cells that mediate postnatal muscle growth and respond differently to temperature based upon aerobic versus anaerobic fiber-type origin. The objective of this study was to determine how temperatures below and above the control, 38°C, affect the fate of satellite cells isolated from the anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b. femoris). At all sampling times, p. major and b. femoris cells accumulated less lipid when incubated at low temperatures and more lipid at elevated temperatures compared to the control. Satellite cells isolated from the p. major were more sensitive to temperature as they accumulated more lipid at elevated temperatures compared to b. femoris cells. Expression of adipogenic genes, CCAAT/enhancer-binding protein β (C/EBPβ) and proliferator-activated receptor gamma (PPARγ) were different within satellite cells isolated from the p. major or b. femoris. At 72 h of proliferation, C/EBPβ expression increased with increasing temperature in both cell types, while PPARγ expression decreased with increasing temperature in p. major satellite cells. At 48 h of differentiation, both C/EBPβ and PPARγ expression increased in the p. major and decreased in the b. femoris, with increasing temperature. Flow cytometry measured apoptotic markers for early apoptosis (Annexin-V-PE) or late apoptosis (7-AAD), showing less than 1% of apoptotic satellite cells throughout all experimental conditions, therefore, apoptosis was considered biologically not significant. The results support that anaerobic p. major satellite cells are more predisposed to adipogenic conversion than aerobic b. femoris cells when thermally challenged. PMID:26341996

Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration.

PubMed

Koide, Masashi; Hagiwara, Yoshihiro; Tsuchiya, Masahiro; Kanzaki, Makoto; Hatakeyama, Hiroyasu; Tanaka, Yukinori; Minowa, Takashi; Takemura, Taro; Ando, Akira; Sekiguchi, Takuya; Yabe, Yutaka; Itoi, Eiji

2018-01-01

Rotator cuff tears (RCTs) are a common shoulder problem in the elderly that can lead to both muscle atrophy and fatty infiltration due to less physical load. Satellite cells, quiescent cells under the basal lamina of skeletal muscle fibers, play a major role in muscle regeneration. However, the myogenic potency of human satellite cells in muscles with fatty infiltration is unclear due to the difficulty in isolating from small samples, and the mechanism of the progression of fatty infiltration has not been elucidated. The purpose of this study was to analyze the population of myogenic and adipogenic cells in disused supraspinatus (SSP) and intact subscapularis (SSC) muscles of the RCTs from the same patients using fluorescence-activated cell sorting. The microstructure of the muscle with fatty infiltration was observed as a whole mount condition under multi-photon microscopy. Myogenic differentiation potential and gene expression were evaluated in satellite cells. The results showed that the SSP muscle with greater fatty infiltration surrounded by collagen fibers compared with the SSC muscle under multi-photon microscopy. A positive correlation was observed between the ratio of muscle volume to fat volume and the ratio of myogenic precursor to adipogenic precursor. Although no difference was observed in the myogenic potential between the two groups in cell culture, satellite cells in the disused SSP muscle showed higher intrinsic myogenic gene expression than those in the intact SSC muscle. Our results indicate that satellite cells from the disused SSP retain sufficient potential of muscle growth despite the fatty infiltration.

Satellites You Can See for Homework

ERIC Educational Resources Information Center

Broderick, Stephen

2012-01-01

Artificial satellites are easily observed most nights when the weather is fine. The website called "Heavens Above" at www.heavens-above.com will help locate these satellites flying over one's location. It also includes how bright they will appear. The direction of travel of each satellite in the night sky also indicates the type of satellite. For…

Stochastic cellular automata model of cell migration, proliferation and differentiation: validation with in vitro cultures of muscle satellite cells.

PubMed

Garijo, N; Manzano, R; Osta, R; Perez, M A

2012-12-07

Cell migration and proliferation has been modelled in the literature as a process similar to diffusion. However, using diffusion models to simulate the proliferation and migration of cells tends to create a homogeneous distribution in the cell density that does not correlate to empirical observations. In fact, the mechanism of cell dispersal is not diffusion. Cells disperse by crawling or proliferation, or are transported in a moving fluid. The use of cellular automata, particle models or cell-based models can overcome this limitation. This paper presents a stochastic cellular automata model to simulate the proliferation, migration and differentiation of cells. These processes are considered as completely stochastic as well as discrete. The model developed was applied to predict the behaviour of in vitro cell cultures performed with adult muscle satellite cells. Moreover, non homogeneous distribution of cells has been observed inside the culture well and, using the above mentioned stochastic cellular automata model, we have been able to predict this heterogeneous cell distribution and compute accurate quantitative results. Differentiation was also incorporated into the computational simulation. The results predicted the myotube formation that typically occurs with adult muscle satellite cells. In conclusion, we have shown how a stochastic cellular automata model can be implemented and is capable of reproducing the in vitro behaviour of adult muscle satellite cells. Copyright © 2012 Elsevier Ltd. All rights reserved.

Dietary tributyrin, an HDAC inhibitor, promotes muscle growth through enhanced terminal differentiation of satellite cells.

PubMed

Murray, Robert L; Zhang, Wei; Iwaniuk, Marie; Grilli, Ester; Stahl, Chad H

2018-05-01

Muscle growth and repair rely on two main mechanisms - myonuclear accretion and subsequent protein accumulation. Altering the ability of muscle resident stem cells (satellite cells) to progress through their myogenic lineage can have a profound effect on lifetime muscle growth and repair. The use of the histone deacetylase (HDAC) inhibitor, butyrate, has had positive outcomes on the in vitro promotion of satellite cell myogenesis. In animal models, the use of butyrate has had promising results in treating myopathic conditions as well as improving growth efficiency, but the impact of dietary butyrate on satellite cells and muscle growth has not been elucidated. We investigated the impact of tributyrin, a butyrate prodrug, on satellite cell activity and muscle growth in a piglet model. Satellite cells from tributyrin-treated piglets had altered myogenic potential, and piglets receiving tributyrin had a ~40% increase in DNA:protein ratio after 21 days, indicating the potential for enhanced muscle growth. To assess muscle growth potential, piglets were supplemented tributyrin (0.5%) during either the neonatal phase (d1-d21) and/or the nursery phase (d21-d58) in a 2 × 2 factorial design. Piglets who received tributyrin during the neonatal phase had improved growth performance at the end of the study and had a ~10% larger loin eye area and muscle fiber cross-sectional area. Tributyrin treatment in the nursery phase alone did not have a significant effect on muscle growth or feed efficiency. These findings suggest that tributyrin is a potent promoter of muscle growth via altered satellite cell myogenesis. © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

The effect of temperature on apoptosis and adipogenesis on skeletal muscle satellite cells derived from different muscle types.

PubMed

Harding, Rachel L; Clark, Daniel L; Halevy, Orna; Coy, Cynthia S; Yahav, Shlomo; Velleman, Sandra G

2015-09-01

Satellite cells are multipotential stem cells that mediate postnatal muscle growth and respond differently to temperature based upon aerobic versus anaerobic fiber-type origin. The objective of this study was to determine how temperatures below and above the control, 38°C, affect the fate of satellite cells isolated from the anaerobic pectoralis major (p. major) or mixed fiber biceps femoris (b. femoris). At all sampling times, p. major and b. femoris cells accumulated less lipid when incubated at low temperatures and more lipid at elevated temperatures compared to the control. Satellite cells isolated from the p. major were more sensitive to temperature as they accumulated more lipid at elevated temperatures compared to b. femoris cells. Expression of adipogenic genes, CCAAT/enhancer-binding protein β (C/EBPβ) and proliferator-activated receptor gamma (PPARγ) were different within satellite cells isolated from the p. major or b. femoris. At 72 h of proliferation, C/EBPβ expression increased with increasing temperature in both cell types, while PPARγ expression decreased with increasing temperature in p. major satellite cells. At 48 h of differentiation, both C/EBPβ and PPARγ expression increased in the p. major and decreased in the b. femoris, with increasing temperature. Flow cytometry measured apoptotic markers for early apoptosis (Annexin-V-PE) or late apoptosis (7-AAD), showing less than 1% of apoptotic satellite cells throughout all experimental conditions, therefore, apoptosis was considered biologically not significant. The results support that anaerobic p. major satellite cells are more predisposed to adipogenic conversion than aerobic b. femoris cells when thermally challenged. © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Evaluation of solar cell materials for a Solar Power Satellite

NASA Technical Reports Server (NTRS)

Glaser, P. E.; Almgren, D. W.; Csigi, K. I.

1980-01-01

Alternative solar cell materials being considered for the solar power satellite are described and price, production, and availability projections through the year 2000 are presented. The chief materials considered are silicon and gallium arsenide.

Ingestion of a Multi-Ingredient Supplement Does Not Alter Exercise-Induced Satellite Cell Responses in Older Men.

PubMed

Snijders, Tim; Bell, Kirsten E; Nederveen, Joshua P; Saddler, Nelson I; Mazara, Nicole; Kumbhare, Dinesh A; Phillips, Stuart M; Parise, Gianni

2018-06-01

Nutritional supplementation can have beneficial effects on body composition, strength, and function in older adults. However, whether the response of satellite cells can be altered by nutritional supplementation in older adults remains unknown. We assessed whether a multi-ingredient protein-based supplement taken over a prolonged period of time could alter the muscle satellite cell response after exercise in older men. Twenty-seven older men [mean ± SD age: 73 ± 1 y; mean ± SD body mass index (kg/m2): 28 ± 1] participated in a randomized double-blind experiment. Participants were randomly divided into an experimental (EXP) group (n = 13) who consumed a multi-ingredient protein-based supplement [30 g whey protein, 2.5 g creatine, 500 IU vitamin D, 400 mg Ca, and 1500 mg n-3 (ω-3) polyunsaturated fatty acids] 2 times/d for 7 wk or a control (CON; 22 g maltodextrin) group (n = 14). After 7 wk of supplementation, all participants performed a single resistance exercise session, and muscle biopsy samples were taken from the vastus lateralis before and 24 and 48 h after exercise. Immunohistochemistry was used to assess the change in type I and II muscle fiber satellite cell content and activation status of the cells. In addition, mRNA expression of the myogenic regulatory factors was determined by using reverse transcriptase-polymerase chain reaction. In response to the single bout of exercise, type I muscle fiber satellite cell content was significantly increased at 24 h (0.132 ± 0.015 and 0.131 ± 0.011 satellite cells/fiber in CON and EXP groups, respectively) and 48 h (0.126 ± 0.010 and 0.120 ± 0.012 satellite cells/fiber in CON and EXP groups, respectively) compared with pre-exercise (0.092 ± 0.007 and 0.118 ± 0.017 satellite cells/fiber in CON and EXP groups, respectively) muscle biopsy samples (P < 0.01), with no difference between the 2 groups. In both groups, we observed no significant changes in type II muscle fiber satellite cell

Characteristics of the Localization of Connexin 43 in Satellite Cells during Skeletal Muscle Regeneration In Vivo

PubMed Central

Ishido, Minenori; Kasuga, Norikatsu

2015-01-01

For myogenesis, new myotubes are formed by the fusion of differentiated myoblasts. In the sequence of events for myotube formation, intercellular communication through gap junctions composed of connexin 43 (Cx43) plays critical roles in regulating the alignment and fusion of myoblasts in advances of myotube formation in vitro. On the other hand, the relationship between the expression patterns of Cx43 and the process of myotube formation in satellite cells during muscle regeneration in vivo remains poorly understood. The present study investigated the relationship between Cx43 and satellite cells in muscle regeneration in vivo. The expression of Cx43 was detected in skeletal muscles on day 1 post-muscle injury, but not in control muscles. Interestingly, the expression of Cx43 was not localized on the inside of the basement membrane of myofibers in the regenerating muscles. Moreover, although the clusters of differentiated satellite cells, which represent a more advanced stage of myotube formation, were observed on the inside of the basement membrane of myofibers in regenerating muscles, the expression of Cx43 was not localized in the clusters of these satellite cells. Therefore, in the present study, it was suggested that Cx43 may not directly contribute to muscle regeneration via satellite cells. PMID:26019374

Effect of incubation temperature on neuropeptide Y and neuropeptide Y receptors in turkey and chicken satellite cells.

PubMed

Clark, Daniel L; McCormick, Janet L; Velleman, Sandra G

2018-05-01

Neuropeptide Y (NPY) is an appetite stimulating peptide released from the central nervous system and impacts the function of many different cell types. A recent transcriptome study showed that NPY expression was altered when turkey breast muscle satellite cells were incubated at low or high temperatures, suggesting NPY may mediate temperature effects on satellite cells. However, to date minimal information exists describing the expression and function of NPY in satellite cells. The objective of this study was to determine how temperature impacts NPY and NPY receptor gene expression in satellite cells isolated from turkeys and chickens with differing genetic lineages. Two broiler and two turkey breast muscle satellite cell lines were incubated at 35, 38 or 41 °C during proliferation and differentiation. In both turkey lines, NPY, and receptors NPY2R and NPY5R expression increased at elevated temperatures after 72 h of proliferation. During differentiation NPY and NPY5R expression increased in both turkey lines with higher temperatures, whereas NPY2R was minimally affected by temperature. In contrast, in both chicken cell lines there were few significant differences for NPY and NPY receptor expression across temperature during proliferation. During differentiation, the temperature effect was different in the two chicken cell lines. In the BPM8 chicken line, there were few differences in NPY and NPY receptors across temperature; whereas elevated temperatures increased NPY, NPY2R, and NPY5R expression in the 708 line. The differences between turkey and chicken lines suggest NPY has species specific satellite cell functions in response to heat stress. Copyright © 2018 Elsevier Inc. All rights reserved.

Modern Inertial and Satellite Navigation Systems

DTIC Science & Technology

1994-05-02

rotor spins, the harder it is to disturb it. This technique is called spin stabilization and it is commonly used for communication satellites. Moder... using a generalization of the complex number called the quaternion . Modem Inertial and Satellite Navigation Systems page 32. 4.2 Exdrson in Pincile...length by an integer. Positive feedback arises from the use of a lasing medium, a gas, liquid, crystal ions, or any of a number of other possibilities

Brain and muscle Arnt-like 1 promotes skeletal muscle regeneration through satellite cell expansion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chatterjee, Somik; Yin, Hongshan; Department of Cardiovascular Medicine, Third Affiliated Hospital, Hebei Medical University, Shijiazhuang 050051, Hebei

Circadian clock is an evolutionarily conserved timing mechanism governing diverse biological processes and the skeletal muscle possesses intrinsic functional clocks. Interestingly, although the essential clock transcription activator, Brain and muscle Arnt-like 1 (Bmal1), participates in maintenance of muscle mass, little is known regarding its role in muscle growth and repair. In this report, we investigate the in vivo function of Bmal1 in skeletal muscle regeneration using two muscle injury models. Bmal1 is highly up-regulated by cardiotoxin injury, and its genetic ablation significantly impairs regeneration with markedly suppressed new myofiber formation and attenuated myogenic induction. A similarly defective regenerative response ismore » observed in Bmal1-null mice as compared to wild-type controls upon freeze injury. Lack of satellite cell expansion accounts for the regeneration defect, as Bmal1{sup −/−} mice display significantly lower satellite cell number with nearly abolished induction of the satellite cell marker, Pax7. Furthermore, satellite cell-derived primary myoblasts devoid of Bmal1 display reduced growth and proliferation ex vivo. Collectively, our results demonstrate, for the first time, that Bmal1 is an integral component of the pro-myogenic response that is required for muscle repair. This mechanism may underlie its role in preserving adult muscle mass and could be targeted therapeutically to prevent muscle-wasting diseases. - Highlights: • Bmal1 is highly inducible by muscle injury and myogenic stimuli. • Genetic ablation of Bmal1 significantly impairs muscle regeneration. • Bmal1 promotes satellite cell expansion during muscle regeneration. • Bmal1-deficient primary myoblasts display attenuated growth and proliferation.« less

Advanced Solar Cells for Satellite Power Systems

NASA Technical Reports Server (NTRS)

Flood, Dennis J.; Weinberg, Irving

1994-01-01

The multiple natures of today's space missions with regard to operational lifetime, orbital environment, cost and size of spacecraft, to name just a few, present such a broad range of performance requirements to be met by the solar array that no single design can suffice to meet them all. The result is a demand for development of specialized solar cell types that help to optimize overall satellite performance within a specified cost range for any given space mission. Historically, space solar array performance has been optimized for a given mission by tailoring the features of silicon solar cells to account for the orbital environment and average operating conditions expected during the mission. It has become necessary to turn to entirely new photovoltaic materials and device designs to meet the requirements of future missions, both in the near and far term. This paper will outline some of the mission drivers and resulting performance requirements that must be met by advanced solar cells, and provide an overview of some of the advanced cell technologies under development to meet them. The discussion will include high efficiency, radiation hard single junction cells; monolithic and mechanically stacked multiple bandgap cells; and thin film cells.

Advanced solar cells for satellite power systems

NASA Astrophysics Data System (ADS)

Flood, Dennis J.; Weinberg, Irving

1994-11-01

The multiple natures of today's space missions with regard to operational lifetime, orbital environment, cost and size of spacecraft, to name just a few, present such a broad range of performance requirements to be met by the solar array that no single design can suffice to meet them all. The result is a demand for development of specialized solar cell types that help to optimize overall satellite performance within a specified cost range for any given space mission. Historically, space solar array performance has been optimized for a given mission by tailoring the features of silicon solar cells to account for the orbital environment and average operating conditions expected during the mission. It has become necessary to turn to entirely new photovoltaic materials and device designs to meet the requirements of future missions, both in the near and far term. This paper will outline some of the mission drivers and resulting performance requirements that must be met by advanced solar cells, and provide an overview of some of the advanced cell technologies under development to meet them. The discussion will include high efficiency, radiation hard single junction cells; monolithic and mechanically stacked multiple bandgap cells; and thin film cells.

Developing a global aeronautical satellite system

NASA Technical Reports Server (NTRS)

Dement, Donald K.

1988-01-01

Arinc, an airline industry-owned and operated company in the United States, has taken steps toward establishing a global aeronautical satellite communications system. Plans call for initiation of a thin-route data operation in 1989, upgrading to establish voice communications via shared spot-beam transponders carried on other satellites, and deploying a worldwide network using dedicated satellites by 1994.

Using Cell Phones From Satellites

NASA Technical Reports Server (NTRS)

Horan, Stephen

2000-01-01

During the past several years, an interest has grown in using commercial telecommunications techniques to supply Telemetry and Command (T&C) services. Recently, the National Aeronautics and Space Administration (NASA) Space Operations Management Office (SOMO) has outlined plans to utilize satellite-based telecommunications services to support space operations in space missions over the next several decades. NASA currently obtains the bulk of its telecommunications services for earth-orbiting satellites via the existing government-owned and controlled Space Network (SN) system. This system consists of the constellation of Tracking and Data Relay Satellites (TDRS) in Geostationary Earth Orbit (GEO) and the associated ground terminals and communications intrastructure. This system is valuable and effective for scientific satellites costing over one million dollars. However, for smaller satellites, this system becomes problematic due to the cost of transponders and support infrastructure. The nominal transponders for using the TDRS cannot be obtained for a cost in dollars, and size, weight, or power that the 3 Corner Satellite project can afford. For these types of nanosatellite missions, alternatives that fit the mission cost and satellite profiles are needed. In particular, low-cost access using existing commercial infrastructure would be useful to mission planners. In particular, the ability to obtain low data rate T&C services would be especially valuable. The nanosatellites generally have low T&C requirements and therefore would benefit from using commercial services that could operate in the 2400 bps - 9600 bps range, especially if contact times longer than the 5 - 10 minute ground station passes could be found.

Myostatin knockout using zinc-finger nucleases promotes proliferation of ovine primary satellite cells in vitro.

PubMed

Salabi, Fatemeh; Nazari, Mahmood; Chen, Qing; Nimal, Jonathan; Tong, Jianming; Cao, Wen G

2014-12-20

Myostatin (MSTN) has previously been shown to negatively regulate the proliferation and differentiation of skeletal muscle cells. Satellite cells are quiescent muscle stem cells that promote muscle growth and repair. Because the mechanism of MSTN in the biology of satellite cells is not well understood, this study was conducted to generate MSTN mono-allelic knockout satellite cells using the zinc-finger nuclease mRNA (MSTN-KO ZFN mRNA) and also to investigate the effect of this disruption on the proliferation and differentiation of sheep primary satellite cells (PSCs). Nineteen biallelic and four mono-allelic knockout cell clones were obtained after sequence analysis. The homologous mono-allelic knockout cells with 5-bp deletion were used to further evaluations. The results demonstrated that mono-allelic knockout of MSTN gene leads to translation inhibition. Real-time quantitative PCR results indicated that knockout of MSTN contributed to an increase in CDK2 and follistatin and a decrease in p21 at the transcript level in proliferation conditions. Moreover, MSTN knockout significantly increased the proliferation of mutant clones (P < 0.01). Consistent with the observed increase in CDK2 and decrease in p21 in cells lacking MSTN, cell cycle analysis showed that MSTN negatively regulated the G1 to S progression. In addition, knockout of myostatin resulted in a remarkable increase in MyoD and MyoG expression under differentiating conditions but had no effect on Myf5 expression. These results expanded our understanding of the regulation mechanism of MSTN. Furthermore, the MSTN-KO ZFN mRNA system in PSCs could be used to generate transgenic sheep in the future.

Neuronal somatic ATP release triggers neuron–satellite glial cell communication in dorsal root ganglia

PubMed Central

Zhang, X.; Chen, Y.; Wang, C.; Huang, L.-Y. M.

2007-01-01

It has been generally assumed that the cell body (soma) of a neuron, which contains the nucleus, is mainly responsible for synthesis of macromolecules and has a limited role in cell-to-cell communication. Using sniffer patch recordings, we show here that electrical stimulation of dorsal root ganglion (DRG) neurons elicits robust vesicular ATP release from their somata. The rate of release events increases with the frequency of nerve stimulation; external Ca2+ entry is required for the release. FM1–43 photoconversion analysis further reveals that small clear vesicles participate in exocytosis. In addition, the released ATP activates P2X7 receptors in satellite cells that enwrap each DRG neuron and triggers the communication between neuronal somata and glial cells. Blocking L-type Ca2+ channels completely eliminates the neuron–glia communication. We further show that activation of P2X7 receptors can lead to the release of tumor necrosis factor-α (TNFα) from satellite cells. TNFα in turn potentiates the P2X3 receptor-mediated responses and increases the excitability of DRG neurons. This study provides strong evidence that somata of DRG neurons actively release transmitters and play a crucial role in bidirectional communication between neurons and surrounding satellite glial cells. These results also suggest that, contrary to the conventional view, neuronal somata have a significant role in cell–cell signaling. PMID:17525149

Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway

PubMed Central

Dai, Jie-Min; Yu, Mu-Xue; Shen, Zhen-Yu; Guo, Chu-Yi; Zhuang, Si-Qi; Qiu, Xiao-Shan

2015-01-01

Signaling through the mammalian target of rapamycin (mTOR) in response to leucine modulates many cellular and developmental processes. However, in the context of satellite cell proliferation and differentiation, the role of leucine and mTORC1 is less known. This study investigates the role of leucine in the process of proliferation and differentiation of primary preterm rat satellite cells, and the relationship with mammalian target of rapamycin complex 1 (mTORC1) activation. Dissociation of primary satellite cells occurred with type I collagenase and trypsin, and purification, via different speed adherence methods. Satellite cells with positive expression of Desmin were treated with leucine and rapamycin. We observed that leucine promoted proliferation and differentiation of primary satellite cells and increased the phosphorylation of mTOR. Rapamycin inhibited proliferation and differentiation, as well as decreased the phosphorylation level of mTOR. Furthermore, leucine increased the expression of MyoD and myogenin while the protein level of MyoD decreased due to rapamycin. However, myogenin expressed no affect by rapamycin. In conclusion, leucine may up-regulate the activation of mTORC1 to promote proliferation and differentiation of primary preterm rat satellite cells. We have shown that leucine promoted the differentiation of myotubes in part through the mTORC1-MyoD signal pathway. PMID:26007333

Rain volume estimation over areas using satellite and radar data

NASA Technical Reports Server (NTRS)

Doneaud, A. A.; Vonderhaar, T. H.

1985-01-01

The feasibility of rain volume estimation over fixed and floating areas was investigated using rapid scan satellite data following a technique recently developed with radar data, called the Area Time Integral (ATI) technique. The radar and rapid scan GOES satellite data were collected during the Cooperative Convective Precipitation Experiment (CCOPE) and North Dakota Cloud Modification Project (NDCMP). Six multicell clusters and cells were analyzed to the present time. A two-cycle oscillation emphasizing the multicell character of the clusters is demonstrated. Three clusters were selected on each day, 12 June and 2 July. The 12 June clusters occurred during the daytime, while the 2 July clusters during the nighttime. A total of 86 time steps of radar and 79 time steps of satellite images were analyzed. There were approximately 12-min time intervals between radar scans on the average.

Barx2 is Expressed in Satellite Cells and is Required for Normal Muscle Growth and Regeneration

PubMed Central

Meech, Robyn; Gonzalez, Katie N.; Barro, Marietta; Gromova, Anastasia; Zhuang, Lizhe; Hulin, Julie-Ann; Makarenkova, Helen P.

2015-01-01

Muscle growth and regeneration are regulated through a series of spatiotemporally dependent signaling and transcriptional cascades. Although the transcriptional program controlling myogenesis has been extensively investigated, the full repertoire of transcriptional regulators involved in this process is far from defined. Various homeodomain transcription factors have been shown to play important roles in both muscle development and muscle satellite cell-dependent repair. Here, we show that the homeodomain factor Barx2 is a new marker for embryonic and adult myoblasts and is required for normal postnatal muscle growth and repair. Barx2 is coexpressed with Pax7, which is the canonical marker of satellite cells, and is upregulated in satellite cells after muscle injury. Mice lacking the Barx2 gene show reduced postnatal muscle growth, muscle atrophy, and defective muscle repair. Moreover, loss of Barx2 delays the expression of genes that control proliferation and differentiation in regenerating muscle. Consistent with the in vivo observations, satellite cell-derived myoblasts cultured from Barx2−/− mice show decreased proliferation and ability to differentiate relative to those from wild-type or Barx2+/− mice. Barx2−/− myoblasts show reduced expression of the differentiation-associated factor myogenin as well as cell adhesion and matrix molecules. Finally, we find that mice lacking both Barx2 and dystrophin gene expression have severe early onset myopathy. Together, these data indicate that Barx2 is an important regulator of muscle growth and repair that acts via the control of satellite cell proliferation and differentiation. PMID:22076929

Role of satellite cells versus myofibers in muscle hypertrophy induced by inhibition of the myostatin/activin signaling pathway.

PubMed

Lee, Se-Jin; Huynh, Thanh V; Lee, Yun-Sil; Sebald, Suzanne M; Wilcox-Adelman, Sarah A; Iwamori, Naoki; Lepper, Christoph; Matzuk, Martin M; Fan, Chen-Ming

2012-08-28

Myostatin and activin A are structurally related secreted proteins that act to limit skeletal muscle growth. The cellular targets for myostatin and activin A in muscle and the role of satellite cells in mediating muscle hypertrophy induced by inhibition of this signaling pathway have not been fully elucidated. Here we show that myostatin/activin A inhibition can cause muscle hypertrophy in mice lacking either syndecan4 or Pax7, both of which are important for satellite cell function and development. Moreover, we show that muscle hypertrophy after pharmacological blockade of this pathway occurs without significant satellite cell proliferation and fusion to myofibers and without an increase in the number of myonuclei per myofiber. Finally, we show that genetic ablation of Acvr2b, which encodes a high-affinity receptor for myostatin and activin A specifically in myofibers is sufficient to induce muscle hypertrophy. All of these findings are consistent with satellite cells playing little or no role in myostatin/activin A signaling in vivo and render support that inhibition of this signaling pathway can be an effective therapeutic approach for increasing muscle growth even in disease settings characterized by satellite cell dysfunction.

Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development.

PubMed

Zhang, Weiya; Xu, Yueyuan; Zhang, Lu; Wang, Sheng; Yin, Binxu; Zhao, Shuhong; Li, Xinyun

2018-06-04

Satellite cells play a key role in the aging, generation, and damage repair of skeletal muscle. The molecular mechanism of satellite cells in these processes remains largely unknown. This study systematically investigated for the first time the characteristics of mouse satellite cells at ten different ages. Results indicated that the number and differentiation capacity of satellite cells decreased with age during skeletal muscle development. Transcriptome analysis revealed that 2,907 genes were differentially expressed at six time points at postnatal stage. WGCNA and GO analysis indicated that 1,739 of the 2,907 DEGs were mainly involved in skeletal muscle development processes. Moreover, the results of WGCNA and protein interaction analysis demonstrated that Tgfβ2, Wnt9a, and Fgfr4 were the key genes responsible for the differentiation of satellite cells. Functional analysis showed that TGFβ2 and WNT9a inhibited, whereas FGFR4 promoted the differentiation of satellite cells. Furthermore, each two of them had a regulatory relationship at the protein level. In vivo study also confirmed that TGFβ2 could regulate the regeneration of skeletal muscle, as well as the expression of WNT9a and FGFR4. Therefore, we concluded that the synergistic effects of TGFβ2, WNT9a, and FGFR4 were responsible for attenuating of the differentiation of aging satellite cells during skeletal muscle development. This study provided new insights into the molecular mechanism of satellite cell development. The target genes and signaling pathways investigated in this study would be useful for improving the muscle growth of livestock or treating muscle diseases in clinical settings. © 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

MMP-14 is necessary but not sufficient for invasion of three-dimensional collagen by human muscle satellite cells

PubMed Central

Lund, Dane K.; Mouly, Vincent

2014-01-01

The twenty-five known matrix metalloproteases (MMPs) and their endogenous inhibitors, tissue inhibitors of metalloproteases (TIMPs), mediate cell invasion through the extracellular matrix (ECM). In a comparative three-dimensional assay, we analyzed human and mouse satellite cells' competence to invade an artificial ECM (collagen I). We identified a single MMP that 1) is expressed by human muscle satellite cells; 2) is induced at the mRNA/protein level by adhesion to collagen I; and 3) is necessary for invasion into a collagen I matrix. Interestingly, murine satellite cells neither express this MMP, nor invade the collagen matrix. However, exogenous human MMP-14 is not sufficient to induce invasion of a collagen matrix by murine cells, emphasizing species differences. PMID:24898588

Aged Muscle Demonstrates Fiber-Type Adaptations in Response to Mechanical Overload, in the Absence of Myofiber Hypertrophy, Independent of Satellite Cell Abundance

PubMed Central

Lee, Jonah D.; Fry, Christopher S.; Mula, Jyothi; Kirby, Tyler J.; Jackson, Janna R.; Liu, Fujun; Yang, Lin; Dupont-Versteegden, Esther E.; McCarthy, John J.

2016-01-01

Although sarcopenia, age-associated loss of muscle mass and strength, is neither accelerated nor exacerbated by depletion of muscle stem cells, satellite cells, we hypothesized that adaptation in sarcopenic muscle would be compromised. To test this hypothesis, we depleted satellite cells with tamoxifen treatment of Pax7CreER-DTA mice at 4 months of age, and 20 months later subjected the plantaris muscle to 2 weeks of mechanical overload. We found myofiber hypertrophy was impaired in aged mice regardless of satellite cell content. Even in the absence of growth, vehicle-treated mice mounted a regenerative response, not apparent in tamoxifen-treated mice. Further, myonuclear accretion occurred in the absence of growth, which was prevented by satellite cell depletion, demonstrating that myonuclear addition is insufficient to drive myofiber hypertrophy. Satellite cell depletion increased extracellular matrix content of aged muscle that was exacerbated by overload, potentially limiting myofiber growth. These results support the idea that satellite cells regulate the muscle environment, and that their loss during aging may contribute to fibrosis, particularly during periods of remodeling. Overload induced a fiber-type composition improvement, independent of satellite cells, suggesting that aged muscle is very responsive to exercise-induced enhancement in oxidative capacity, even with an impaired hypertrophic response. PMID:25878030

Spanish Earth Observation Satellite System

NASA Astrophysics Data System (ADS)

Borges, A.; Cerezo, F.; Fernandez, M.; Lomba, J.; Lopez, M.; Moreno, J.; Neira, A.; Quintana, C.; Torres, J.; Trigo, R.; Urena, J.; Vega, E.; Vez, E.

2010-12-01

The Spanish Ministry of Industry, Tourism and Trade (MITyC) and the Ministry of Defense (MoD) signed an agreement in 2007 for the development of a "Spanish Earth Observation Satellite System" based, in first instance, on two satellites: a high resolution optical satellite, called SEOSAT/Ingenio, and a radar satellite based on SAR technology, called SEOSAR/Paz. SEOSAT/Ingenio is managed by MITyC through the Centre for the Development of Industrial Technology (CDTI), with technical and contractual support from the European Space Agency (ESA). HISDESA T together with the Spanish Instituto Nacional de Técnica Aeroespacial (INTA, National Institute for Aerospace Technology) will be responsible for the in-orbit operation and the commercial operation of both satellites, and for the technical management of SEOSAR/Paz on behalf of the MoD. In both cases EADS CASA Espacio (ECE) is the prime contractor leading the industrial consortia. The ground segment development will be assigned to a Spanish consortium. This system is the most important contribution of Spain to the European Programme Global Monitoring for Environment and Security, GMES. This paper presents the Spanish Earth Observation Satellite System focusing on SEOSA T/Ingenio Programme and with special emphasis in the potential contribution to the ESA Third Party Missions Programme and to the Global Monitoring for Environment and Security initiative (GMES) Data Access.

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies.

PubMed

Feng, Xuesong; Naz, Faiza; Juan, Aster H; Dell'Orso, Stefania; Sartorelli, Vittorio

2018-04-19

Immunofluorescence is an effective method that helps to identify different cell types on tissue sections. In order to study the desired cell population, antibodies for specific cell markers are applied on tissue sections. In adult skeletal muscle, satellite cells (SCs) are stem cells that contribute to muscle repair and regeneration. Therefore, it is important to visualize and trace the satellite cell population under different physiological conditions. In resting skeletal muscle, SCs reside between the basal lamina and myofiber plasma membrane. A commonly used marker for identifying SCs on the myofibers or in cell culture is the paired box protein Pax7. In this article, an optimized Pax7 immunofluorescence protocol on skeletal muscle sections is presented that minimizes non-specific staining and background. Another antibody that recognizes a protein (laminin) of the basal lamina was also added to help identify SCs. Similar protocols can also be used to perform double or triple labeling with Pax7 and antibodies for additional proteins of interest.

Stress hormone is implicated in satellite-caller associations and sexual selection in the Great Plains toad.

PubMed

Leary, Christopher J; Garcia, Apryl M; Knapp, Rosemary

2006-10-01

The effects of androgens on male-typical traits suggest that variation among males in circulating levels can play a major role in sexual selection. We examined whether variation in vocal attractiveness is attributable to differences in androgen levels among Great Plains toads (Bufo cognatus). We found that noncalling "satellite" males practicing an alternative mating tactic were more likely to associate with males producing long calls. However, callers with satellites did not have higher androgen levels than callers without satellites. Rather, callers with satellites had significantly lower corticosterone (CORT) levels than callers without satellites. A CORT manipulation experiment suggested that differences in calls for males with and without satellites were related to differences in CORT levels. Furthermore, there was a negative correlation between CORT level and call duration within most nights of chorus activity. However, the correlation was weak for the pooled data (across all nights), suggesting that local environmental and/or social factors also affect call duration. Last, we show that females preferred broadcast calls of longer duration, characteristic of males with satellites and low CORT. These results imply that satellites optimize their reproductive success by associating with males producing long calls. However, this association should negatively affect the fitness of attractive callers.

MicroRNA-133 Controls Brown Adipose Determination in Skeletal Muscle Satellite Cells by Targeting Prdm16

PubMed Central

Yin, Hang; Pasut, Alessandra; Soleimani, Vahab D.; Bentzinger, C. Florian; Antoun, Ghadi; Thorn, Stephanie; Seale, Patrick; Fernando, Pasan; van IJcken, Wilfred; Grosveld, Frank; Dekemp, Robert A.; Boushel, Robert; Harper, Mary-Ellen; Rudnicki, Michael A.

2013-01-01

SUMMARY Brown adipose tissue (BAT) is an energy-dispensing thermogenic tissue that plays an important role in balancing energy metabolism. Lineage-tracing experiments indicate that brown adipocytes are derived from myogenic progenitors during embryonic development. However, adult skeletal muscle stem cells (satellite cells) have long been considered uniformly determined toward the myogenic lineage. Here, we report that adult satellite cells give rise to brown adipocytes and that microRNA-133 regulates the choice between myogenic and brown adipose determination by targeting the 3′UTR of Prdm16. Antagonism of microRNA-133 during muscle regeneration increases uncoupled respiration, glucose uptake, and thermogenesis in local treated muscle and augments whole-body energy expenditure, improves glucose tolerance, and impedes the development of diet-induced obesity. Finally, we demonstrate that miR-133 levels are downregulated in mice exposed to cold, resulting in de novo generation of satellite cell-derived brown adipocytes. Therefore, microRNA-133 represents an important therapeutic target for the treatment of obesity. PMID:23395168

Rat soleus muscle satellite cells during the recovery after gravitational unloading

NASA Astrophysics Data System (ADS)

Turtikova, Olga; Shenkman, Boris; Altaeva, Erzhena; Leinsoo, Toomas

In this study the attempt was made to assess alterations of rat soleus satellite cell (SC) population during muscle regrowth after 14-day gravitational unloading (using the hindlimb suspension model). Myofiber size increases during the recovery period. SCs are supposed to participate in muscle growth by fusion with myofibers and supplying them with new myonuclei [Mitchell PO, Pavlath GK, 2001; Oishi Y., 2008]. Other points of view are known about SC participation in the recovery of atrophied muscle mass during the readaptation period [Bruusgaard J.C. et al., 2011; Jackson JR et al., 2012]. After 2 weeks of hindlimb suspension mki67 expression was fivefold lower as compared to control animals and increased gradually up to 28 times by the day 7 of reloading. Cdh15 was decreased after hindlimb unloading and rose from the 1st day of reloading. The expression reached control level to the day 7th of reloading. Cellular response was going on concurrently with the spike of IGF-1 blood level and the increase in muscle IGF-1 concentration. It is possible that in the early days of reloading period differentiation and fusion of satellite cells which were active by the end of hindlimb suspension occurred. Satellite cell incorporation was assessed by counting the amount of BrdU+ myonuclei under myofiber dystrophin layer. It came more intensively in the 1st day of readaptation. It is in accordance with the 4,5 time increase in myogenin expression as compared to hindlimb suspended animals detected at the same time point. Myogenin expression 3 fold decreased by 3rd day of readaptation. We observed only the tendency of resizing but no significant changes in in myonuclear domain size. The number of myonuclei per myofiber cross section was decreased after hindlimb suspension and was not restored by the day 14th of readaptation. Cdh15 and myogenin expression at some extent stabilized after 7 days of readaptation, but high mki67 level pointed to intensive proliferation, which could

Regenerative fuel cell study for satellites in GEO orbit

NASA Technical Reports Server (NTRS)

Vandine, Leslie; Gonzalez-Sanabria, Olga; Levy, Alexander

1987-01-01

The results of a 12 month study to identify high performance regenerative hydrogen-oxygen fuel cell concepts for geosynchronous satellite application are summarized. Emphasis was placed on concepts with the potential for high energy density and passive means for water and heat management to maximize system reliability. Both polymer membrane and alkaline electrolyte fuel cells were considered, with emphasis on the alkaline cell because of its high performance, advanced state of development, and proven ability to operate in a launch and space environment. Three alkaline system concepts were studied. Results indicate that using near term technology energy densities between 46 and 52 watt-hour/lb can be achieved at efficiencies of 55 percent. Using advanced light weight cell construction which was achieved in experimental cells, composite tankage material for the reactant gases and the reversible stack concept, system energy densities of 115 watt-hours/lb can be projected.

Utilizing a TDRS satellite for direct broadcast satellite-radio propagation experiments and demonstrations

NASA Technical Reports Server (NTRS)

Hollansworth, James E.

1993-01-01

The NASA/VOA Direct Broadcast Satellite-Radio (DBS-R) Program will be using a NASA Tracking Data Relay Satellite (TDRS) satellite at 62 deg. West longitude to conduct live satellite S-band propagation experiments and demonstrations of satellite sound broadcasting over the next two years (1993-1994). The NASA/VOA DBS-R program has applied intensive effort to garner domestic and international support for the DBS-R concept. An S-band DBS-R allocation was achieved for Region 2 at WARC-92 held in Spain. With this allocation, the DBS-R program now needs to conduct S-band propagation experiments and systems demonstrations that will assist in the development of planning approaches for the use of Broadcast Satellite Service (Sound) frequency bands prior to the planning conference called for by WARC-92. These activities will also support receiver concept development applied to qualities ranging from AM to Monophonic FM, Stereophonic FM, Monophonic CD, and Stereophonic CD quality.

Cost Consideration for Future Communications Satellite

NASA Astrophysics Data System (ADS)

Iida, Takashi

2002-01-01

This paper discusses the cost driving factors of the future communications satellite rather than discussing its cost itself directly, in terms of development period of time, services, and R&D by government. In the first, a period of time for development of a communications system is discussed in comparison of satellite communications system with a terrestrial communications system. Generally speaking, the terrestrial communications system is developed in a short period. Especially, the recent network related IT technology changes very rapidly, like so-called as "Dog Year". On the other hand, it takes a long time, more than several years, to develop a satellite communications system. This paper will discuss this time period of development is how to influence the system realization in various cases. In the second, the service related cost is discussed. First, a mobile communications satellite system is considered as an example. The tremendous penetration speed of the terrestrial cellular phones prevents from the success of the mobile satellite communications system. The success of the mobile satellite communications system depends on how early and user friendly to develop its user terminals. Second, the broadcasting service is described as a successful example. It is described that the satellite broadcasting has a very competitive advantage to the terrestrial broadcasting service from the cost point of view. Finally, the cost of the technology R&D for the future communication satellite by the government is discussed. A model of the future communications satellite for next 30 years has been proposed(1)(2). As an example, this paper estimates the satellite cost of the 60 Gbps range of capacity which is called as 1.5G satellite, where the capacity of the second generation Internet satellite (2G) is 50-500 Gbps per satellite. In the paper, the R&D plan of the future communications satellite will be discussed as a next R&D project to the first generation Internet

The effect of syndecan-4 and glypican-1 knockdown on the proliferation and differentiation of turkey satellite cells differing in age and growth rates.

PubMed

Velleman, Sandra G; Clark, Daniel L; Tonniges, Jeffrey R

2018-09-01

Posthatch skeletal muscle growth requires myogenic satellite cells and the dynamic expression of cell membrane-associated proteins. The membrane associated heparan sulfate proteoglycans, syndecan-4 and glypican-1, link the satellite cell niche to the intracellular environment. Sydnecan-4 and glypican-1 are differentially expressed with age in turkey satellite cells and their over-expression impacts both satellite cell proliferation and differentiation, but their effect on satellite cells from lines with different growth potentials is not known. The objective of the current study was to determine if syndecan-4 and glypican-1 regulation of satellite cell proliferation and differentiation is affected by age and growth selection. Pectoralis major satellite cells isolated at 1 d, 7 and 16-wk of age from a Randombred Control 2 (RBC2) line and a 16-wk body weight (F) line selected from the RBC2 line turkeys were studied. Syndecan-4 and glypican-1 expression was knocked down in both lines. The F-line cells proliferated faster than RBC2 line cells regardless of age, while differentiation tended to be greater in RBC2 line cells than F-line cells at each age. Syndecan-4 knockdown decreased proliferation at 7- and 16-wk but not 1 d cells, and increased differentiation at 1 d and 7 wk but not 16 wk cells. Glypican-1 knockdown differentially affected proliferation depending on cell age, whereas differentiation was decreased for 7- and 16-wk but not 1 d cells. These data suggest syndecan-4 and glypican-1 differentially affected satellite cell function in an age-dependent manner, but had little impact on differences in proliferation and differentiation due to growth selection. Copyright © 2018. Published by Elsevier Inc.

Adapted physical exercise enhances activation and differentiation potential of satellite cells in the skeletal muscle of old mice.

PubMed

Cisterna, Barbara; Giagnacovo, Marzia; Costanzo, Manuela; Fattoretti, Patrizia; Zancanaro, Carlo; Pellicciari, Carlo; Malatesta, Manuela

2016-05-01

During ageing, a progressive loss of skeletal muscle mass and a decrease in muscle strength and endurance take place, in the condition termed sarcopenia. The mechanisms of sarcopenia are complex and still unclear; however, it is known that muscle atrophy is associated with a decline in the number and/or efficiency of satellite cells, the main contributors to muscle regeneration. Physical exercise proved beneficial in sarcopenia; however, knowledge of the effect of adapted physical exercise on the myogenic properties of satellite cells in aged muscles is limited. In this study the amount and activation state of satellite cells as well as their proliferation and differentiation potential were assessed in situ by morphology, morphometry and immunocytochemistry at light and transmission electron microscopy on 28-month-old mice submitted to adapted aerobic physical exercise on a treadmill. Sedentary age-matched mice served as controls, and sedentary adult mice were used as a reference for an unperturbed control at an age when the capability of muscle regeneration is still high. The effect of physical exercise in aged muscles was further analysed by comparing the myogenic potential of satellite cells isolated from old running and old sedentary mice using an in vitro system that allows observation of the differentiation process under controlled experimental conditions. The results of this ex vivo and in vitro study demonstrated that adapted physical exercise increases the number and activation of satellite cells as well as their capability to differentiate into structurally and functionally correct myotubes (even though the age-related impairment in myotube formation is not fully reversed): this evidence further supports adapted physical exercise as a powerful, non-pharmacological approach to counteract sarcopenia and the age-related deterioration of satellite cell capabilities even at very advanced age. © 2016 Anatomical Society.

A short review of variants calling for single-cell-sequencing data with applications.

PubMed

Wei, Zhuohui; Shu, Chang; Zhang, Changsheng; Huang, Jingying; Cai, Hongmin

2017-11-01

The field of single-cell sequencing is fleetly expanding, and many techniques have been developed in the past decade. With this technology, biologists can study not only the heterogeneity between two adjacent cells in the same tissue or organ, but also the evolutionary relationships and degenerative processes in a single cell. Calling variants is the main purpose in analyzing single cell sequencing (SCS) data. Currently, some popular methods used for bulk-cell-sequencing data analysis are tailored directly to be applied in dealing with SCS data. However, SCS requires an extra step of genome amplification to accumulate enough quantity for satisfying sequencing needs. The amplification yields large biases and thus raises challenge for using the bulk-cell-sequencing methods. In order to provide guidance for the development of specialized analyzed methods as well as using currently developed tools for SNS, this paper aims to bridge the gap. In this paper, we firstly introduced two popular genome amplification methods and compared their capabilities. Then we introduced a few popular models for calling single-nucleotide polymorphisms and copy-number variations. Finally, break-through applications of SNS were summarized to demonstrate its potential in researching cell evolution. Copyright © 2017 Elsevier Ltd. All rights reserved.

Small satellite debris catalog maintenance issues

NASA Technical Reports Server (NTRS)

Jackson, Phoebe A.

1991-01-01

The United States Space Command (USSPACECOM) is a unified command of the Department of Defense, and one of its tasks is to detect, track, identify, and maintain a catalog of all man-made objects in Earth orbit. This task is called space surveillance, and the most important tool for space surveillance is the satellite catalog. The command's reasons for performing satellite catalog maintenance is presented. A satellite catalog is described, and small satellite-debris catalog-maintenance issues are identified. The underlying rationale is to describe the catalog maintenance services so that the members of the community can use them with assurance.

Voluntary wheel running increases satellite cell abundance and improves recovery from disuse in gastrocnemius muscles from mice.

PubMed

Brooks, Matthew J; Hajira, Ameena; Mohamed, Junaith S; Alway, Stephen E

2018-06-01

Reloading of atrophied muscles after hindlimb suspension unloading (HSU) can induce injury and prolong recovery. Low-impact exercise, such as voluntary wheel running, has been identified as a nondamaging rehabilitation therapy in rodents, but its effects on muscle function, morphology, and satellite cell activity after HSU are unclear. This study tested the hypothesis that low-impact wheel running would increase satellite cell proliferation and improve recovery of muscle structure and function after HSU in mice. Young adult male and female C57BL/6 mice ( n = 6/group) were randomly placed into five groups. These included HSU without recovery (HSU), normal ambulatory recovery for 14 days after HSU (HSU+NoWR), and voluntary wheel running recovery for 14 days after HSU (HSU+WR). Two control groups were used: nonsuspended mouse cage controls (Control) and voluntary wheel running controls (ControlWR). Satellite cell activation was evaluated by providing mice 5-bromo-2'-deoxyuridine (BrdU) in their drinking water. As expected, HSU significantly reduced in vivo maximal force, decreased in vivo fatigability, and decreased type I and IIa myosin heavy chain (MHC) abundance in plantarflexor muscles. HSU+WR mice significantly improved plantarflexor fatigue resistance, increased type I and IIa MHC abundance, increased fiber cross-sectional area, and increased the percentage of type I and IIA muscle fibers in the gastrocnemius muscle. HSU+WR mice also had a significantly greater percentage of BrdU-positive and Pax 7-positive nuclei inside muscle fibers and a greater MyoD-to-Pax 7 protein ratio compared with HSU+NoWR mice. The mechanotransduction protein Yes-associated protein (YAP) was elevated with reloading after HSU, but HSU+WR mice had lower levels of the inactive phosphorylated YAP serine127 , which may have contributed to increased satellite cell activation with reloading after HSU. These results indicate that voluntary wheel running increased YAP signaling and satellite

Alternative Reproductive Tactics Arising from a Continuous Behavioral Trait: Callers versus Satellites in Field Crickets.

PubMed

Rotenberry, John T; Swanger, Elizabeth; Zuk, Marlene

2015-04-01

Alternative reproductive tactics may arise when natural enemies use sexual signals to locate the signaler. In field crickets, elevated costs to male calling due to acoustically orienting parasitoid flies create opportunity for an alternative tactic, satellite behavior, where noncalling males intercept females attracted to callers. Although the caller-satellite system in crickets that risk detection by parasitoids resembles distinct behavioral phenotypes, a male's propensity to behave as caller or satellite can be a continuously variable trait over several temporal scales, and an individual may pursue alternate tactics at different times. We modeled a caller-satellite-parasitoid system as a spatially explicit interaction among male and female crickets using individual-based simulation. Males varied in their propensity to call versus behave as a satellite from one night to the next. We varied mortality, density, sex ratio, and female mating behavior, and recorded lifetime number of mates as a function of a male's probability of calling (vs. acting as a satellite) along a gradient in parasitism risk. Frequently, the optimal behavior switched abruptly from being pure caller (call every night) to pure satellite (never call) as parasitism rate increased. However, mixed strategies prevailed even with high parasitism risk under conditions of higher background mortality rate, decreasing density, increasing female-biased sex ratio, and increasing female choosiness. In natural populations, high parasitoid pressure alone would be unlikely to yield fixation of pure satellite behavior.

Spiral ganglion degeneration and hearing loss as a consequence of satellite cell death in saposin B-deficient mice.

PubMed

Akil, Omar; Sun, Ying; Vijayakumar, Sarath; Zhang, Wujuan; Ku, Tiffany; Lee, Chi-Kyou; Jones, Sherri; Grabowski, Gregory A; Lustig, Lawrence R

2015-02-18

Saposin B (Sap B) is an essential activator protein for arylsulfatase A in the hydrolysis of sulfatide, a lipid component of myelin. To study Sap B's role in hearing and balance, a Sap B-deficient (B(-/-)) mouse was evaluated. At both light and electron microscopy (EM) levels, inclusion body accumulation was seen in satellite cells surrounding spiral ganglion (SG) neurons from postnatal month 1 onward, progressing into large vacuoles preceding satellite cell degeneration, and followed by SG degeneration. EM also revealed reduced or absent myelin sheaths in SG neurons from postnatal month 8 onwards. Hearing loss was initially seen at postnatal month 6 and progressed thereafter for frequency-specific stimuli, whereas click responses became abnormal from postnatal month 13 onward. The progressive hearing loss correlated with the accumulation of inclusion bodies in the satellite cells and their subsequent degeneration. Outer hair cell numbers and efferent function measures (distortion product otoacoustic emissions and contralateral suppression) were normal in the B(-/-) mice throughout this period. Alcian blue staining of SGs demonstrated that these inclusion bodies corresponded to sulfatide accumulation. In contrast, changes in the vestibular system were much milder, but caused severe physiologic deficits. These results demonstrate that loss of Sap B function leads to progressive sulfatide accumulation in satellite cells surrounding the SG neurons, leading to satellite cell degeneration and subsequent SG degeneration with a resultant loss of hearing. Relative sparing of the efferent auditory and vestibular neurons suggests that alternate glycosphingolipid metabolic pathways predominate in these other systems. Copyright © 2015 the authors 0270-6474/15/353263-13$15.00/0.

Satellite tracking of threatened species

USGS Publications Warehouse

Williams, M.; Lunsford, A.; Ellis, D.; Robinson, J.; Coronado, P.; Campbell, W.

1998-01-01

In 1990, a joint effort of two U.S. federal agencies, NASA Goddard Space Flight Center (GSFC) and the Patuxent Wildlife Research Center, began. We initially joined forces in a project that used satellite telemetry to discover the winter home of a tiny dwindling population of Siberian Cranes. Since then several projects have emerged, and a web site was created to follow some of these activities. This web site is called the Satellite Tracking of Threatened Species and its location is http://sdcd.gsfc.nasa.gov/ISTO/satellite_tracking. It describes the overall program, and links you to three subsections that describe the projects in more detail: Satellite Direct Readout, Birdtracks, and Birdworld.

A POGLUT1 mutation causes a muscular dystrophy with reduced Notch signaling and satellite cell loss.

PubMed

Servián-Morilla, Emilia; Takeuchi, Hideyuki; Lee, Tom V; Clarimon, Jordi; Mavillard, Fabiola; Area-Gómez, Estela; Rivas, Eloy; Nieto-González, Jose L; Rivero, Maria C; Cabrera-Serrano, Macarena; Gómez-Sánchez, Leonardo; Martínez-López, Jose A; Estrada, Beatriz; Márquez, Celedonio; Morgado, Yolanda; Suárez-Calvet, Xavier; Pita, Guillermo; Bigot, Anne; Gallardo, Eduard; Fernández-Chacón, Rafael; Hirano, Michio; Haltiwanger, Robert S; Jafar-Nejad, Hamed; Paradas, Carmen

2016-11-01

Skeletal muscle regeneration by muscle satellite cells is a physiological mechanism activated upon muscle damage and regulated by Notch signaling. In a family with autosomal recessive limb-girdle muscular dystrophy, we identified a missense mutation in POGLUT1 (protein O-glucosyltransferase 1), an enzyme involved in Notch posttranslational modification and function. In vitro and in vivo experiments demonstrated that the mutation reduces O-glucosyltransferase activity on Notch and impairs muscle development. Muscles from patients revealed decreased Notch signaling, dramatic reduction in satellite cell pool and a muscle-specific α-dystroglycan hypoglycosylation not present in patients' fibroblasts. Primary myoblasts from patients showed slow proliferation, facilitated differentiation, and a decreased pool of quiescent PAX7 + cells. A robust rescue of the myogenesis was demonstrated by increasing Notch signaling. None of these alterations were found in muscles from secondary dystroglycanopathy patients. These data suggest that a key pathomechanism for this novel form of muscular dystrophy is Notch-dependent loss of satellite cells. © 2016 The Authors. Published under the terms of the CC BY 4.0 license.

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16.

PubMed

Yin, Hang; Pasut, Alessandra; Soleimani, Vahab D; Bentzinger, C Florian; Antoun, Ghadi; Thorn, Stephanie; Seale, Patrick; Fernando, Pasan; van Ijcken, Wilfred; Grosveld, Frank; Dekemp, Robert A; Boushel, Robert; Harper, Mary-Ellen; Rudnicki, Michael A

2013-02-05

Brown adipose tissue (BAT) is an energy-dispensing thermogenic tissue that plays an important role in balancing energy metabolism. Lineage-tracing experiments indicate that brown adipocytes are derived from myogenic progenitors during embryonic development. However, adult skeletal muscle stem cells (satellite cells) have long been considered uniformly determined toward the myogenic lineage. Here, we report that adult satellite cells give rise to brown adipocytes and that microRNA-133 regulates the choice between myogenic and brown adipose determination by targeting the 3'UTR of Prdm16. Antagonism of microRNA-133 during muscle regeneration increases uncoupled respiration, glucose uptake, and thermogenesis in local treated muscle and augments whole-body energy expenditure, improves glucose tolerance, and impedes the development of diet-induced obesity. Finally, we demonstrate that miR-133 levels are downregulated in mice exposed to cold, resulting in de novo generation of satellite cell-derived brown adipocytes. Therefore, microRNA-133 represents an important therapeutic target for the treatment of obesity. Copyright © 2013 Elsevier Inc. All rights reserved.

Inadequate satellite cell replication compromises muscle regrowth following postnatal nutrient restriction

USDA-ARS?s Scientific Manuscript database

Perinatal growth impairment permanently compromises skeletal muscle mass. The present study assessed the contribution of muscle satellite cell replicative capacity to this deficit. Mouse dams were fed either a low protein (LP, n=7) or control (C, n=6) diet during lactation. Pups were weaned at 21 d ...

Then Why Do They Call Earth the Blue Planet?

NASA Technical Reports Server (NTRS)

2005-01-01

While the most common photographs of Earth taken from space show the planet covered in blue water, NASA has managed to produce detailed color images, using satellite imagery, that show the remarkable variation of colors that actually make up the oceanic surface. An ocean s color is determined by the interaction of surface waters with sunlight, and surface waters can contain any number of different particles and dissolved substances, which could then change the color. Then Why Do They Call Earth the Blue Planet? The particles are mostly phytoplankton, the microscopic, single-celled ocean plants that are the primary food source for much marine life. Remote detection of phytoplankton provides information about the uptake and cycling of carbon by the ocean through photosynthesis, as well as the overall health of the water. Inorganic particles and substances dissolved in the water also affect its color, particularly in coastal regions. Satellite images can be used to calculate the concentrations of these materials in surface waters, as well as the levels of biological activity. The satellites allow a global view that is not available from ship or shore. NASA s orbiting satellites offer a unique vantage point for studying the oceans. By resolving the biological, chemical, and physical conditions in surface waters, they have allowed the oceanographic community to make huge leaps in its understanding of oceanographic processes on regional and global fronts. The study of ocean color, in particular, has been integral in helping researchers understand the natural and human-induced changes in the global environment and establishing the role of the oceans in the biochemical cycles of elements that influence the climate and the distribution of life on Earth.

Myonuclear Domain Flexibility Challenges Rigid Assumptions on Satellite Cell Contribution to Skeletal Muscle Fiber Hypertrophy.

PubMed

Murach, Kevin A; Englund, Davis A; Dupont-Versteegden, Esther E; McCarthy, John J; Peterson, Charlotte A

2018-01-01

Satellite cell-mediated myonuclear accretion is thought to be required for skeletal muscle fiber hypertrophy, and even drive hypertrophy by preceding growth. Recent studies in humans and rodents provide evidence that challenge this axiom. Specifically, Type 2 muscle fibers reliably demonstrate a substantial capacity to hypertrophy in the absence of myonuclear accretion, challenging the notion of a tightly regulated myonuclear domain (i.e., area that each myonucleus transcriptionally governs). In fact, a "myonuclear domain ceiling", or upper limit of transcriptional output per nucleus to support hypertrophy, has yet to be identified. Satellite cells respond to muscle damage, and also play an important role in extracellular matrix remodeling during loading-induced hypertrophy. We postulate that robust satellite cell activation and proliferation in response to mechanical loading is largely for these purposes. Future work will aim to elucidate the mechanisms by which Type 2 fibers can hypertrophy without additional myonuclei, the extent to which Type 1 fibers can grow without myonuclear accretion, and whether a true myonuclear domain ceiling exists.

First micro-satellite and new enhanced small satellite series in DFH Satellite Co. Ltd.

NASA Astrophysics Data System (ADS)

Zhang, Xiaomin; Xie, Bin; Dai, Shoulun; Zhang, Weiwen; Hu, Gefeng; Lan, Ding

2007-06-01

As one important department of CAST (Chinese Academy of Space Technology), with responsibility for small and micro-satellite development, DFH Satellite Co. Ltd. (DFHSat) manufactured and launched six small satellites from 2000 to 2004. Nowadays, DFHSat is developing micro-satellite and new enhanced small satellite series. The first micro-satellite as a basic type is named HummerSat-1. HummerSat-1 is three-axis stabilized with orbit control capability. Information and power control are implemented through an on-board network, GaAs solar cell and Li-ion battery are adopted to obtain and storage power, S-band TT&C and data transmission works are used. The payload of HummerSat-1 has a weight of 60 kg and power consumption of 200 W. The enhanced small satellite series based on technology of HummerSat-1 is planned by DFHSat. It would be aiming to form a continuative product spectrum of 80-200 kg. The enhanced series is focused on satisfying different missions, including earth remote sensing, communication, science exploration, etc.

Heterochromatin-Encoded Satellite RNAs Induce Breast Cancer.

PubMed

Zhu, Quan; Hoong, Nien; Aslanian, Aaron; Hara, Toshiro; Benner, Christopher; Heinz, Sven; Miga, Karen H; Ke, Eugene; Verma, Sachin; Soroczynski, Jan; Yates, John R; Hunter, Tony; Verma, Inder M

2018-06-07

Heterochromatic repetitive satellite RNAs are extensively transcribed in a variety of human cancers, including BRCA1 mutant breast cancer. Aberrant expression of satellite RNAs in cultured cells induces the DNA damage response, activates cell cycle checkpoints, and causes defects in chromosome segregation. However, the mechanism by which satellite RNA expression leads to genomic instability is not well understood. Here we provide evidence that increased levels of satellite RNAs in mammary glands induce tumor formation in mice. Using mass spectrometry, we further show that genomic instability induced by satellite RNAs occurs through interactions with BRCA1-associated protein networks required for the stabilization of DNA replication forks. Additionally, de-stabilized replication forks likely promote the formation of RNA-DNA hybrids in cells expressing satellite RNAs. These studies lay the foundation for developing novel therapeutic strategies that block the effects of non-coding satellite RNAs in cancer cells. Copyright © 2018 Elsevier Inc. All rights reserved.

Proinflammatory Cytokine Tumor Necrosis Factor (TNF)-like Weak Inducer of Apoptosis (TWEAK) Suppresses Satellite Cell Self-renewal through Inversely Modulating Notch and NF-κB Signaling Pathways*

PubMed Central

Ogura, Yuji; Mishra, Vivek; Hindi, Sajedah M.; Kuang, Shihuan; Kumar, Ashok

2013-01-01

Satellite cell self-renewal is an essential process to maintaining the robustness of skeletal muscle regenerative capacity. However, extrinsic factors that regulate self-renewal of satellite cells are not well understood. Here, we demonstrate that TWEAK cytokine reduces the proportion of Pax7+/MyoD− cells (an index of self-renewal) on myofiber explants and represses multiple components of Notch signaling in satellite cell cultures. The number of Pax7+ cells is significantly increased in skeletal muscle of TWEAK knock-out (KO) mice compared with wild-type in response to injury. Furthermore, Notch signaling is significantly elevated in cultured satellite cells and in regenerating myofibers of TWEAK-KO mice. Forced activation of Notch signaling through overexpression of the Notch1 intracellular domain (N1ICD) rescued the TWEAK-mediated inhibition of satellite cell self-renewal. TWEAK also activates the NF-κB transcription factor in satellite cells and inhibition of NF-κB significantly improved the number of Pax7+ cells in TWEAK-treated cultures. Furthermore, our results demonstrate that a reciprocal interaction between NF-κB and Notch signaling governs the inhibitory effect of TWEAK on satellite cell self-renewal. Collectively, our study demonstrates that TWEAK suppresses satellite cell self-renewal through activating NF-κB and repressing Notch signaling. PMID:24151074

Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-κB signaling pathways.

PubMed

Ogura, Yuji; Mishra, Vivek; Hindi, Sajedah M; Kuang, Shihuan; Kumar, Ashok

2013-12-06

Satellite cell self-renewal is an essential process to maintaining the robustness of skeletal muscle regenerative capacity. However, extrinsic factors that regulate self-renewal of satellite cells are not well understood. Here, we demonstrate that TWEAK cytokine reduces the proportion of Pax7(+)/MyoD(-) cells (an index of self-renewal) on myofiber explants and represses multiple components of Notch signaling in satellite cell cultures. The number of Pax7(+) cells is significantly increased in skeletal muscle of TWEAK knock-out (KO) mice compared with wild-type in response to injury. Furthermore, Notch signaling is significantly elevated in cultured satellite cells and in regenerating myofibers of TWEAK-KO mice. Forced activation of Notch signaling through overexpression of the Notch1 intracellular domain (N1ICD) rescued the TWEAK-mediated inhibition of satellite cell self-renewal. TWEAK also activates the NF-κB transcription factor in satellite cells and inhibition of NF-κB significantly improved the number of Pax7(+) cells in TWEAK-treated cultures. Furthermore, our results demonstrate that a reciprocal interaction between NF-κB and Notch signaling governs the inhibitory effect of TWEAK on satellite cell self-renewal. Collectively, our study demonstrates that TWEAK suppresses satellite cell self-renewal through activating NF-κB and repressing Notch signaling.

Association of pKi-67 with satellite DNA of the human genome in early G1 cells.

PubMed

Bridger, J M; Kill, I R; Lichter, P

1998-01-01

pKi-67 is a nucleolar antigen that provides a specific marker for proliferating cells. It has been shown previously that pKi-67's distribution varies in a cell cycle-dependent manner: it coats all chromosomes during mitosis, accumulates in nuclear foci during G1 phase (type I distribution) and localizes within nucleoli in late G1 S and G2 phase (type II distribution). Although no function has as yet been ascribed to pKi-67, it has been found associated with centromeres in G1. In the present study the distribution pattern of pKi-67 during G1 in human dermal fibroblasts (HDFs) was analysed in more detail. Synchronization experiments show that in very early G1 cells pKi-67 coincides with virtually all satellite regions analysed, i.e. with centromeric (alpha-satellite), telomeric (minisatellite) and heterochromatic blocks (satellite III) on chromosomes 1 and Y (type Ia distribution). In contrast, later in the G1 phase, a smaller fraction of satellite DNA regions are found collocalized with pKi-67 foci (type Ib distribution). When all pKi-67 becomes localized within nucleoli, even fewer satellite regions remain associated with the pKi-67 staining. However, all centromeric and short arm regions of the acrocentric chromosomes, which are in very close proximity to or even contain the rRNA genes, are collocalized with anti-pKi-67 staining throughout the remaining interphase of the cell cycle. Thus, our data demonstrate that during post-mitotic reformation and nucleogenesis there is a progressive decline in the fraction of specific satellite regions of DNA that remain associated with pKi-67. This may be relevant to nucleolar reformation following mitosis.

Solar array experiments on the SPHINX satellite. [Space Plasma High voltage INteraction eXperiment satellite

NASA Technical Reports Server (NTRS)

Stevens, N. J.

1974-01-01

The Space Plasma, High Voltage Interaction Experiment (SPHINX) is the name given to an auxiliary payload satellite scheduled to be launched in January 1974. The principal experiments carried on this satellite are specifically designed to obtain the engineering data on the interaction of high voltage systems with the space plasma. The classes of experiments are solar array segments, insulators, insulators with pin holes and conductors. The satellite is also carrying experiments to obtain flight data on three new solar array configurations: the edge illuminated-multijunction cells, the teflon encased cells, and the violet cells.

Antennas for mobile satellite communications

NASA Technical Reports Server (NTRS)

Huang, John

1991-01-01

A NASA sponsored program, called the Mobile Satellite (MSAT) system, has prompted the development of several innovative antennas at L-band frequencies. In the space segment of the MSAT system, an efficient, light weight, circularly polarized microstrip array that uses linearly polarized elements was developed as a multiple beam reflector feed system. In the ground segment, a low-cost, low-profile, and very efficient microstrip Yagi array was developed as a medium-gain mechanically steered vehicle antenna. Circularly shaped microstrip patches excited at higher-order modes were also developed as low-gain vehicle antennas. A more recent effort called for the development of a 20/30 GHz mobile terminal antenna for future-generation mobile satellite communications. To combat the high insertion loss encountered at 20/30 GHz, series-fed Monolithic Microwave Integrated Circuit (MMIC) microstrip array antennas are currently being developed. These MMIC arrays may lead to the development of several small but high-gain Ka-band antennas for the Personal Access Satellite Service planned for the 2000s.

A new higher performance NGO satellite for direct audio/video broadcast

NASA Astrophysics Data System (ADS)

Briskman, Robert D.; Foust, Joseph V.

2010-03-01

A three satellite constellation using non-geostationary orbits (NGO) was launched in the latter half of 2000. It is providing direct satellite broadcasting audio and video services to over 9 million mobile and fixed subscribers throughout North America. The constellation will be augmented with a geostationary satellite called FM-5 in 2009, providing increased availability to the user with this "Hybrid" constellation. Effort has recently started on replacement satellites for the original NGO satellites, the first one called FM-6. This new satellite will be placed in a different orbital plane from the original ones providing a constellation which brings further operational improvements. The paper describes the new satellite which has twice the prime and radio frequency (RF) power than the original and a 9 m diameter aperture transmit antenna whose shaped antenna beam delivers much higher effective isotropic radiated power (EIRP). Other technology advances used in the satellite such as electric propulsion, precision star sensors, and enhanced performing lithium-ion batteries are also described in the paper.

Xin, an actin binding protein, is expressed within muscle satellite cells and newly regenerated skeletal muscle fibers.

PubMed

Hawke, Thomas J; Atkinson, Daniel J; Kanatous, Shane B; Van der Ven, Peter F M; Goetsch, Sean C; Garry, Daniel J

2007-11-01

Xin is a muscle-specific actin binding protein of which its role and regulation within skeletal muscle is not well understood. Here we demonstrate that Xin mRNA is robustly upregulated (>16-fold) within 12 h of skeletal muscle injury and is localized to the muscle satellite cell population. RT-PCR confirmed the expression pattern of Xin during regeneration, as well as within primary muscle myoblast cultures, but not other known stem cell populations. Immunohistochemical staining of single myofibers demonstrate Xin expression colocalized with the satellite cell marker Syndecan-4 further supporting the mRNA expression of Xin in satellite cells. In situ hybridization of regenerating muscle 5-7 days postinjury illustrates Xin expression within newly regenerated myofibers. Promoter-reporter assays demonstrate that known myogenic transcription factors [myocyte enhancer factor-2 (MEF2), myogenic differentiation-1 (MyoD), and myogenic factor-5 (Myf-5)] transactivate Xin promoter constructs supporting the muscle-specific expression of Xin. To determine the role of Xin within muscle precursor cells, proliferation, migration, and differentiation analysis using Xin, short hairpin RNA (shRNA) were undertaken in C2C12 myoblasts. Reducing endogenous Xin expression resulted in a 26% increase (P < 0.05) in cell proliferation and a 20% increase (P < 0.05) in myoblast migratory capacity. Skeletal muscle myosin heavy chain protein levels were increased (P < 0.05) with Xin shRNA administration; however, this was not accompanied by changes in myoglobin protein (another marker of differentiation) nor overt morphological differences relative to differentiating control cells. Taken together, the present findings support the hypothesis that Xin is expressed within muscle satellite cells during skeletal muscle regeneration and is involved in the regulation of myoblast function.

Solar satellites

NASA Astrophysics Data System (ADS)

Poher, C.

A reference system design, projected costs, and the functional concepts of a satellite solar power system (SSPS) for converting sunlight falling on solar panels of a satellite in GEO to a multi-GW beam which could be received by a rectenna on earth are outlined. Electricity transmission by microwaves has been demonstrated, and a reference design system for supplying 5 GW dc to earth was devised. The system will use either monocrystalline Si or concentrator GaAs solar cells for energy collection in GEO. Development is still needed to improve the lifespan of the cells. Currently, the cell performance degrades 50 percent in efficiency after 7-8 yr in space. Each SSPS satellite would weigh either 34,000 tons (Si) or 51,000 tons (GaAs), thereby requiring the fabrication of a heavy lift launch vehicle or a single-stage-to-orbit transport in order to minimize launch costs. Costs for the solar panels have been estimated at $500/kW using the GaAs technology, with transport costs for materials to GEO being $40/kg.

A high-fidelity satellite ephemeris program for Earth satellites in eccentric orbits

NASA Technical Reports Server (NTRS)

Simmons, David R.

1990-01-01

A program for mission planning called the Analytic Satellite Ephemeris Program (ASEP), produces projected data for orbits that remain fairly close to the Earth. ASEP does not take into account lunar and solar perturbations. These perturbations are accounted for in another program called GRAVE, which incorporates more flexible means of input for initial data, provides additional kinds of output information, and makes use of structural programming techniques to make the program more understandable and reliable. GRAVE was revised, and a new program called ORBIT was developed. It is divided into three major phases: initialization, integration, and output. Results of the program development are presented.

Land mobile satellite system requirements

NASA Astrophysics Data System (ADS)

Kiesling, J. D.

1983-05-01

A Land Mobile Satellite System (LMSS) provides voice, data and related communications services to moving vehicles and persons. Communications between the mobiles and satellite are in the 806-890 MHz band. The satellite translates these signals to a ""fixed services band'' such as 14/12 GHz band (Ku-band), and communicates in this band with fixed terminals called gateways. The gateways are located at convenient places such as telephone switches (which provide entry into the national telephone system), dispatcher headquarters, computer centers, etc. Communications are therefore principally mobile to fixed. A third communications link, also at Ku-band, is needed between the satellite and a single fixed ground station. This link provides satellite command, telemetry and ranging and also provides a network control function. The latter, through a common signalling system, receives requests and assigns channel slots, and otherwise controls, monitors and polices the network and collects billing information.

Land mobile satellite system requirements

NASA Technical Reports Server (NTRS)

Kiesling, J. D.

1983-01-01

A Land Mobile Satellite System (LMSS) provides voice, data and related communications services to moving vehicles and persons. Communications between the mobiles and satellite are in the 806-890 MHz band. The satellite translates these signals to a ""fixed services band'' such as 14/12 GHz band (Ku-band), and communicates in this band with fixed terminals called gateways. The gateways are located at convenient places such as telephone switches (which provide entry into the national telephone system), dispatcher headquarters, computer centers, etc. Communications are therefore principally mobile to fixed. A third communications link, also at Ku-band, is needed between the satellite and a single fixed ground station. This link provides satellite command, telemetry and ranging and also provides a network control function. The latter, through a common signalling system, receives requests and assigns channel slots, and otherwise controls, monitors and polices the network and collects billing information.

Satellite antenna management system and method

NASA Technical Reports Server (NTRS)

Leath, Timothy T (Inventor); Azzolini, John D (Inventor)

1999-01-01

The antenna management system and method allow a satellite to communicate with a ground station either directly or by an intermediary of a second satellite, thus permitting communication even when the satellite is not within range of the ground station. The system and method employ five major software components, which are the control and initialization module, the command and telemetry handler module, the contact schedule processor module, the contact state machining module, and the telemetry state machine module. The control and initialization module initializes the system and operates the main control cycle, in which the other modules are called. The command and telemetry handler module handles communication to and from the ground station. The contact scheduler processor module handles the contact entry schedules to allow scheduling of contacts with the second satellite. The contact and telemetry state machine modules handle the various states of the satellite in beginning, maintaining and ending contact with the second satellite and in beginning, maintaining and ending communication with the satellite.

Development of a nitric oxide-releasing analogue of the muscle relaxant guaifenesin for skeletal muscle satellite cell myogenesis.

PubMed

Wang, Guqi; Burczynski, Frank J; Hasinoff, Brian B; Zhang, Kaidong; Lu, Qilong; Anderson, Judy E

2009-01-01

Nitric oxide (NO) mediates activation of satellite precursor cells to enter the cell cycle. This provides new precursor cells for skeletal muscle growth and muscle repair from injury or disease. Targeting a new drug that specifically delivers NO to muscle has the potential to promote normal function and treat neuromuscular disease, and would also help to avoid side effects of NO from other treatment modalities. In this research, we examined the effectiveness of the NO donor, iosorbide dinitrate (ISDN), and a muscle relaxant, methocarbamol, in promoting satellite cell activation assayed by muscle cell DNA synthesis in normal adult mice. The work led to the development of guaifenesin dinitrate (GDN) as a new NO donor for delivering nitric oxide to muscle. The results revealed that there was a strong increase in muscle satellite cell activation and proliferation, demonstrated by a significant 38% rise in DNA synthesis after a single transdermal treatment with the new compound for 24 h. Western blot and immunohistochemistry analyses showed that the markers of satellite cell myogenesis, expression of myf5, myogenin, and follistatin, were increased after 24 h oral administration of the compound in adult mice. This research extends our understanding of the outcomes of NO-based treatments aimed at promoting muscle regeneration in normal tissue. The potential use of such treatment for conditions such as muscle atrophy in disuse and aging, and for the promotion of muscle tissue repair as required after injury or in neuromuscular diseases such as muscular dystrophy, is highlighted.

Regulation of myogenesis and skeletal muscle regeneration: effects of oxygen levels on satellite cell activity.

PubMed

Chaillou, Thomas; Lanner, Johanna T

2016-12-01

Reduced oxygen (O 2 ) levels (hypoxia) are present during embryogenesis and exposure to altitude and in pathologic conditions. During embryogenesis, myogenic progenitor cells reside in a hypoxic microenvironment, which may regulate their activity. Satellite cells are myogenic progenitor cells localized in a local environment, suggesting that the O 2 level could affect their activity during muscle regeneration. In this review, we present the idea that O 2 levels regulate myogenesis and muscle regeneration, we elucidate the molecular mechanisms underlying myogenesis and muscle regeneration in hypoxia and depict therapeutic strategies using changes in O 2 levels to promote muscle regeneration. Severe hypoxia (≤1% O 2 ) appears detrimental for myogenic differentiation in vitro, whereas a 3-6% O 2 level could promote myogenesis. Hypoxia impairs the regenerative capacity of injured muscles. Although it remains to be explored, hypoxia may contribute to the muscle damage observed in patients with pathologies associated with hypoxia (chronic obstructive pulmonary disease, and peripheral arterial disease). Hypoxia affects satellite cell activity and myogenesis through mechanisms dependent and independent of hypoxia-inducible factor-1α. Finally, hyperbaric oxygen therapy and transplantation of hypoxia-conditioned myoblasts are beneficial procedures to enhance muscle regeneration in animals. These therapies may be clinically relevant to treatment of patients with severe muscle damage.-Chaillou, T. Lanner, J. T. Regulation of myogenesis and skeletal muscle regeneration: effects of oxygen levels on satellite cell activity. © FASEB.

To call or not to call--that is the question (while driving).

PubMed

Tractinsky, Noam; Ram, Efrat Soffer; Shinar, David

2013-07-01

We studied whether decisions to engage in cell phone conversation while driving and the consequences of such decisions are related to the driver's age, to the road conditions (demands of the driving task), and to the driver's role in initiating the phone call (i.e. the driver as caller vs. as receiver). Two experiments were performed in a driving simulator in which driver age, road conditions and phone conversation, as a secondary task, were manipulated. Engagement in cell phone conversations, performance in the driving and the conversation tasks, and subjective effort assessment were recorded. In general, drivers were more willing to accept incoming calls than to initiate calls. In addition, older and younger drivers were more susceptible to the deleterious effects of phone conversations while driving than middle aged/experienced drivers. While older drivers were aware of this susceptibility by showing sensitivity to road conditions before deciding whether to engage in a call or not, young drivers showed no such sensitivity. The results can guide the development of young driver training programs and point at the need to develop context-aware management systems of in-vehicle cell phone conversations. Copyright © 2013 Elsevier Ltd. All rights reserved.

FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration

PubMed Central

Castiglioni, Alessandra; Basso, Veronica; Vezzoli, Michela; Monno, Antonella; Almada, Albert E.; Mondino, Anna; Wagers, Amy J.; Manfredi, Angelo A.; Rovere-Querini, Patrizia

2015-01-01

Muscle injury induces a classical inflammatory response in which cells of the innate immune system rapidly invade the tissue. Macrophages are prominently involved in this response and required for proper healing, as they are known to be important for clearing cellular debris and supporting satellite cell differentiation. Here, we sought to assess the role of the adaptive immune system in muscle regeneration after acute damage. We show that T lymphocytes are transiently recruited into the muscle after damage and appear to exert a pro-myogenic effect on muscle repair. We observed a decrease in the cross-sectional area of regenerating myofibers after injury in Rag2-/- γ-chain-/- mice, as compared to WT controls, suggesting that T cell recruitment promotes muscle regeneration. Skeletal muscle infiltrating T lymphocytes were enriched in CD4+CD25+FOXP3+ cells. Direct exposure of muscle satellite cells to in vitro induced Treg cells effectively enhanced their expansion, and concurrently inhibited their myogenic differentiation. In vivo, the recruitment of Tregs to acutely injured muscle was limited to the time period of satellite expansion, with possibly important implications for situations in which inflammatory conditions persist, such as muscular dystrophies and inflammatory myopathies. We conclude that the adaptive immune system, in particular T regulatory cells, is critically involved in effective skeletal muscle regeneration. Thus, in addition to their well-established role as regulators of the immune/inflammatory response, T regulatory cells also regulate the activity of skeletal muscle precursor cells, and are instrumental for the proper regeneration of this tissue. PMID:26039259

IGF-1 colocalizes with muscle satellite cells following acute exercise in humans.

PubMed

Grubb, Amanda; Joanisse, Sophie; Moore, Daniel R; Bellamy, Leeann M; Mitchell, Cameron J; Phillips, Stuart M; Parise, Gianni

2014-04-01

Insulin-like growth factor-1 (IGF-1) regulates stem cell proliferation and differentiation in vitro. The aim of this study was to quantify the change in satellite cell (SC) specific IGF-1 colocalization following exercise. We observed a significant increase (p < 0.05) in the percentage of SC with IGF-1 colocalization from baseline to 72 h after a bout of resistance exercise. This strongly supports a role for IGF-1 in human SC function following exercise.

Evidence of heterogeneity within bovine satellite cells isolated from young and adult animals.

PubMed

Li, J; Gonzalez, J M; Walker, D K; Hersom, M J; Ealy, A D; Johnson, S E

2011-06-01

Satellite cells are a heterogeneous population of myogenic precursors responsible for muscle growth and repair in mammals. The objectives of the experiment were to examine the growth rates and degree of heterogeneity within bovine satellite cells (BSC) isolated from young and adult animals. The BSC were harvested from the semimembranosus of young (4.3 ± 0.5 d) and adult (estimated 24 to 27 mo) cattle and cultured en masse. Young animal BSC re-enter the cell cycle sooner and reach maximal 5-ethynyl-2'-deoxyuridine (EdU) incorporation earlier (P < 0.05) than adult contemporaries. Adult BSC contain fewer (P < 0.05) MyoD and myogenin immunopositive nuclei than BSC isolated from young animals after 3, 4, and 5 d in culture. These results indicate that BSC from young animals activate, proliferate, and differentiate sooner than isolates from adult animals. Lineage heterogeneity within BSC was examined using antibodies specific for Pax7 and Myf5, lineage markers of satellite cells, and myoblasts. Immunocytochemistry revealed the majority of Pax7-expressing BSC also express Myf5; a minor population (~5%) fails to exhibit Myf5 immunoreactivity. The percentage of Pax7:Myf5 BSC from young animals decreases sooner (P < 0.05) in culture than adult BSC, indicating a more rapid rate of muscle fiber formation. A subpopulation immunopositive for Myf5 only was identified in both ages of BSC isolates. The growth kinetics and heterogeneity of young BSC was further evaluated by clonal analysis. Single cell clones were established and analyzed after 10 d. Colonies segregated into 2 groups based upon population doubling time. Immunostaining of the slow-growing colonies (population doubling time ≥ 3 d) revealed that a portion exhibited asymmetric distribution of the lineage markers Pax7 and Myf5, similar to self-renewable mouse muscle stem cells. In summary, these results offer insight into the heterogeneity of BSC and provide evidence for subtle differences between rodent and bovine

Reinforcement learning for resource allocation in LEO satellite networks.

PubMed

Usaha, Wipawee; Barria, Javier A

2007-06-01

In this paper, we develop and assess online decision-making algorithms for call admission and routing for low Earth orbit (LEO) satellite networks. It has been shown in a recent paper that, in a LEO satellite system, a semi-Markov decision process formulation of the call admission and routing problem can achieve better performance in terms of an average revenue function than existing routing methods. However, the conventional dynamic programming (DP) numerical solution becomes prohibited as the problem size increases. In this paper, two solution methods based on reinforcement learning (RL) are proposed in order to circumvent the computational burden of DP. The first method is based on an actor-critic method with temporal-difference (TD) learning. The second method is based on a critic-only method, called optimistic TD learning. The algorithms enhance performance in terms of requirements in storage, computational complexity and computational time, and in terms of an overall long-term average revenue function that penalizes blocked calls. Numerical studies are carried out, and the results obtained show that the RL framework can achieve up to 56% higher average revenue over existing routing methods used in LEO satellite networks with reasonable storage and computational requirements.

Candidate solar cell materials for photovoltaic conversion in a solar power satellite /SPS/

NASA Technical Reports Server (NTRS)

Glaser, P. E.; Almgren, D. W.

1978-01-01

In recognition of the obstacles to solar-generated baseload power on earth, proposals have been made to locate solar power satellites in geosynchronous earth orbit (GEO), where solar energy would be available 24 hours a day during most of the time of the year. In an SPS, the electricity produced by solar energy conversion will be fed to microwave generators forming part of a planar phase-array transmitting antenna. The antenna is designed to precisely direct a microwave beam of very low intensity to one or more receiving antennas at desired locations on earth. At the receiving antenna, the microwave energy will be safely and efficiently reconverted to electricity and then be transmitted to consumers. An SPS system will include a number of satellites in GEO. Attention is given to the photovoltaic option for solar energy conversion in GEO, solar cell requirements, the availability of materials, the implication of large production volumes, requirements for high-volume manufacture of solar cell arrays, and the effects of concentration ratio on solar cell array area.

Mobile satellite service in the United States

NASA Technical Reports Server (NTRS)

Agnew, Carson E.; Bhagat, Jai; Hopper, Edwin A.; Kiesling, John D.; Exner, Michael L.; Melillo, Lawrence; Noreen, Gary K.; Parrott, Billy J.

1988-01-01

Mobile satellite service (MSS) has been under development in the United States for more than two decades. The service will soon be provided on a commercial basis by a consortium of eight U.S. companies called the American Mobile Satellite Consortium (AMSC). AMSC will build a three-satellite MSS system that will offer superior performance, reliability and cost effectiveness for organizations requiring mobile communications across the U.S. The development and operation of MSS in North America is being coordinated with Telesat Canada and Mexico. AMSC expects NASA to provide launch services in exchange for capacity on the first AMSC satellite for MSAT-X activities and for government demonstrations.

Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation

PubMed Central

McKenzie, Alec I.; D'Lugos, Andrew C.; Saunders, Michael J.; Gworek, Keith D.; Luden, Nicholas D.

2016-01-01

The central purpose of this study was to evaluate the fiber type-specific satellite cell and myonuclear responses of endurance-trained cyclists to a block of intensified training, when supplementing with carbohydrate (CHO) vs. carbohydrate-protein (PRO). In a crossover design, endurance-trained cyclists (n = 8) performed two consecutive training periods, once supplementing with CHO (de facto “control” condition) and the other with PRO. Each training period consisted of 10 days of intensified cycle training (ICT–120% increase in average training duration) followed by 10 days of recovery (RVT–reduced volume training; 33% volume reduction vs. normal training). Skeletal muscle biopsies were obtained from the vastus lateralis before and after ICT and again following RVT. Immunofluorescent microscopy was used to quantify SCs (Pax7+), myonuclei (DAPI+), and myosin heavy chain I (MyHC I). Data are expressed as percent change ± 90% confidence limits. The 10-day block of ICTCHO increased MyHC I SC content (35 ± 28%) and myonuclear density (16 ± 6%), which remained elevated following RVTCHO (SC = 69 ± 50% vs. PRE; Nuclei = 17 ± 15% vs. PRE). MyHC II SC and myonuclei were not different following ICTCHO, but were higher following RVTCHO (SC = +33 ± 31% vs. PRE; Nuclei = 15 ± 14% vs. PRE), indicating a delayed response compared to MyHC I fibers. The MyHC I SC pool increased following ICTPRO (37 ± 37%), but without a concomitant increase in myonuclei. There were no changes in MyHC II SC or myonuclei following ICTPRO. Collectively, these trained endurance cyclists possessed a relatively large pool of SCs that facilitated rapid (MyHC I) and delayed (MyHC II) satellite cell proliferation and myonuclear accretion under carbohydrate conditions. The current findings strengthen the growing body of evidence demonstrating alterations in satellite cell number in the absence of hypertrophy. Satellite cell pool expansion is typically viewed as an advantageous response to

Satellite Radar Measures Tohoku, Japan Earthquake

NASA Image and Video Library

2011-04-01

This radar image, called a coseismic interferogram, using satellite data from the European Space Agency Envisat depicts ground displacements resulting from the March 11, 2011, magnitude 9.0 Tohoku earthquake in Japan.

Timation 3 satellite. [artificial satellite for navigation, space radiation, and time transfer applications

NASA Technical Reports Server (NTRS)

Bartholomew, C. A.

1972-01-01

The characteristics of the Timation 3 satellite are discussed. A diagram of the basic structure is provide to show the solar panels, navigation and telemetry antennas, gravity gradient booms, and solar cell experiments. The specific application of the satellite for time management or time transfer for navigation purposes is reported. Various measurements and experiments conducted by the satellite are described.

Satellite communications system 'Tyulpan'

NASA Astrophysics Data System (ADS)

Tchuyan, R. K.; Tarasov, E. V.; Belousov, A. P.; Balyk, V. M.; Kovtunenko, V. M.; Morozov, V. A.; Andreev, V. A.; v'yunenko, K. A.

1993-10-01

A concept of the satellite communication system called 'Tyulpan' (because or its tulip-resembling shape) is considered. This conception envisages the use of six satellites-retranslators installed on high-latitude elliptic orbits. Such a system can provide the communication for mean- and high-latitude region of Europe, Asia, and America. For the communication, super small ground stations of 0.4 m in diameter can be used. In the development of system conception, the already existing technical solutions and possibility of conversion or existing installations of military destination were taken into account. Therefore, the system considered can be realized at the earliest possible date.

Reducing juvenile delinquency with automated cell phone calls.

PubMed

Burraston, Bert O; Bahr, Stephen J; Cherrington, David J

2014-05-01

Using a sample of 70 juvenile probationers (39 treatment and 31 controls), we evaluated the effectiveness of a rehabilitation program that combined cognitive-behavioral training and automated phone calls. The cognitive-behavioral training contained six 90-min sessions, one per week, and the phone calls occurred twice per day for the year following treatment. Recidivism was measured by whether they were rearrested and the total number of rearrests during the 1st year. To test the impact of the phone calls, those who received phone calls were divided into high and low groups depending on whether they answered more or less than half of their phone calls. Those who completed the class and answered at least half of their phone calls were less likely to have been arrested and had fewer total arrests.

Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication

PubMed Central

Mackey, Abigail L.; Rasmussen, Lotte K.; Kadi, Fawzi; Schjerling, Peter; Helmark, Ida C.; Ponsot, Elodie; Aagaard, Per; Durigan, João Luiz Q.; Kjaer, Michael

2016-01-01

With this study we investigated the role of nonsteroidal anti-inflammatory drugs (NSAIDs) in human skeletal muscle regeneration. Young men ingested NSAID [1200 mg/d ibuprofen (IBU)] or placebo (PLA) daily for 2 wk before and 4 wk after an electrical stimulation–induced injury to the leg extensor muscles of one leg. Muscle biopsies were collected from the vastus lateralis muscles before and after stimulation (2.5 h and 2, 7, and 30 d) and were assessed for satellite cells and regeneration by immunohistochemistry and real-time RT-PCR, and we also measured telomere length. After injury, and compared with PLA, IBU was found to augment the proportion of ActiveNotch1+ satellite cells at 2 d [IBU, 29 ± 3% vs. PLA, 19 ± 2% (means ± sem)], satellite cell content at 7 d [IBU, 0.16 ± 0.01 vs. PLA, 0.12 ± 0.01 (Pax7+ cells/fiber)], and to expedite muscle repair at 30 d. The PLA group displayed a greater proportion of embryonic myosin+ fibers and a residual ∼2-fold increase in mRNA levels of matrix proteins (all P < 0.05). Endomysial collagen was also elevated with PLA at 30 d. Minimum telomere length shortening was not observed. In conclusion, ingestion of NSAID has a potentiating effect on Notch activation of satellite cells and muscle remodeling during large-scale regeneration of injured human skeletal muscle.—Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., Kjaer, M. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication. PMID:26936358

Preface: BeiDou Navigation Satellite System (BDS)/GNSS+: New developments and emerging applications

NASA Astrophysics Data System (ADS)

Jin, Shuanggen

2017-12-01

The China's BeiDou Navigation Satellite System (BDS) has been developed and operated well with over 25 launched satellites in 2017, including fifteen Medium Earth orbit (MEO) satellites, five geostationary Earth orbit (GEO) satellites and five inclined geosynchronous orbit (IGSO) satellites. Together with the United States' GPS, European Union's Galileo and Russia's GLONASS as well as other regional augmentation systems, e.g., Indian Regional Navigation Satellite System (IRNSS) and Japan Quasi-Zenith Satellite System (QZSS), more emerging applications of multi-Global Navigation Satellite Systems (GNSS) will be exploited and realized in the coming years. The papers in this issue of Advances in Space Research present new advances in the system, techniques and emerging applications of BDS/GNSS+. These papers were from an open call and a special call for participants at the 8th China Satellite Navigation Conference (CSNC 2017) held on May 23-25, 2017, Shanghai, China. This conference series provides a good platform for academic and technique exchanges as well as collaboration in satellite navigation. CSNC 2017 was well attend with more than 3000 participants and over 800 papers in 12 sessions.

Loss of Ptpn11 (Shp2) drives satellite cells into quiescence

PubMed Central

Griger, Joscha; Schneider, Robin; Lahmann, Ines; Schöwel, Verena; Keller, Charles; Spuler, Simone; Nazare, Marc; Birchmeier, Carmen

2017-01-01

The equilibrium between proliferation and quiescence of myogenic progenitor and stem cells is tightly regulated to ensure appropriate skeletal muscle growth and repair. The non-receptor tyrosine phosphatase Ptpn11 (Shp2) is an important transducer of growth factor and cytokine signals. Here we combined complex genetic analyses, biochemical studies and pharmacological interference to demonstrate a central role of Ptpn11 in postnatal myogenesis of mice. Loss of Ptpn11 drove muscle stem cells out of the proliferative and into a resting state during muscle growth. This Ptpn11 function was observed in postnatal but not fetal myogenic stem cells. Furthermore, muscle repair was severely perturbed when Ptpn11 was ablated in stem cells due to a deficit in stem cell proliferation and survival. Our data demonstrate a molecular difference in the control of cell cycle withdrawal in fetal and postnatal myogenic stem cells, and assign to Ptpn11 signaling a key function in satellite cell activity. DOI: http://dx.doi.org/10.7554/eLife.21552.001 PMID:28463680

Insulin-like growth factor-I extends in vitro replicative life span of skeletal muscle satellite cells by enhancing G1/S cell cycle progression via the activation of phosphatidylinositol 3'-kinase/Akt signaling pathway

NASA Technical Reports Server (NTRS)

Chakravarthy, M. V.; Abraha, T. W.; Schwartz, R. J.; Fiorotto, M. L.; Booth, F. W.

2000-01-01

Interest is growing in methods to extend replicative life span of non-immortalized stem cells. Using the insulin-like growth factor I (IGF-I) transgenic mouse in which the IGF-I transgene is expressed during skeletal muscle development and maturation prior to isolation and during culture of satellite cells (the myogenic stem cells of mature skeletal muscle fibers) as a model system, we elucidated the underlying molecular mechanisms of IGF-I-mediated enhancement of proliferative potential of these cells. Satellite cells from IGF-I transgenic muscles achieved at least five additional population doublings above the maximum that was attained by wild type satellite cells. This IGF-I-induced increase in proliferative potential was mediated via activation of the phosphatidylinositol 3'-kinase/Akt pathway, independent of mitogen-activated protein kinase activity, facilitating G(1)/S cell cycle progression via a down-regulation of p27(Kip1). Adenovirally mediated ectopic overexpression of p27(Kip1) in exponentially growing IGF-I transgenic satellite cells reversed the increase in cyclin E-cdk2 kinase activity, pRb phosphorylation, and cyclin A protein abundance, thereby implicating an important role for p27(Kip1) in promoting satellite cell senescence. These observations provide a more complete dissection of molecular events by which increased local expression of a growth factor in mature skeletal muscle fibers extends replicative life span of primary stem cells than previously known.

Effect of β-hydroxy-β-methylbutyrate on miRNA expression in differentiating equine satellite cells exposed to hydrogen peroxide.

PubMed

Chodkowska, Karolina A; Ciecierska, Anna; Majchrzak, Kinga; Ostaszewski, Piotr; Sadkowski, Tomasz

2018-01-01

Skeletal muscle injury activates satellite cells to initiate processes of proliferation, differentiation, and hypertrophy in order to regenerate muscle fibers. The number of microRNAs and their target genes are engaged in satellite cell activation. β-Hydroxy-β-methylbutyrate (HMB) is known to prevent exercise-induced muscle damage. The purpose of this study was to evaluate the effect of HMB on miRNA and relevant target gene expression in differentiating equine satellite cells exposed to H 2 O 2 . We hypothesized that HMB may regulate satellite cell activity, proliferation, and differentiation, hence attenuate the pathological processes induced during an in vitro model of H 2 O 2 -related injury by changing the expression of miRNAs. Equine satellite cells (ESC) were isolated from the samples of skeletal muscle collected from young horses. ESC were treated with HMB (24 h) and then exposed to H 2 O 2 (1 h). For the microRNA and gene expression assessment microarrays, technique was used. Identified miRNAs and genes were validated using real-time qPCR. Cell viability, oxidative stress, and cell damage were measured using colorimetric method and flow cytometry. Analysis of miRNA and gene profile in differentiating ESC pre-incubated with HMB and then exposed to H 2 O 2 revealed difference in the expression of 27 miRNAs and 4740 genes, of which 344 were potential target genes for identified miRNAs. Special attention was focused on differentially expressed miRNAs and their target genes involved in processes related to skeletal muscle injury. Western blot analysis showed protein protection in HMB-pre-treated group compared to control. The viability test confirmed that HMB enhanced cell survival after the hydrogen peroxide exposition. Our results suggest that ESC pre-incubated with HMB and exposed to H 2 O 2 could affect expression on miRNA levels responsible for skeletal muscle development, cell proliferation and differentiation, and activation of tissue repair after

Satellite cell proliferation in adult skeletal muscle

NASA Technical Reports Server (NTRS)

Morrison, Paul R. (Inventor); Thomason, Donald B. (Inventor); Stancel, George M. (Inventor); Booth, Frank W. (Inventor)

1995-01-01

Novel methods of retroviral-mediated gene transfer for the in vivo corporation and stable expression of eukaryotic or prokaryotic foreign genes in tissues of living animals is described. More specifically, methods of incorporating foreign genes into mitotically active cells are disclosed. The constitutive and stable expression of E. coli .beta.-galactosidase gene under the promoter control of the Moloney murine leukemia virus long terminal repeat is employed as a particularly preferred embodiment, by way of example, establishes the model upon which the incorporation of a foreign gene into a mitotically-active living eukaryotic tissue is based. Use of the described methods in therapeutic treatments for genetic diseases, such as those muscular degenerative diseases, is also presented. In muscle tissue, the described processes result in genetically-altered satellite cells which proliferate daughter myoblasts which preferentially fuse to form a single undamaged muscle fiber replacing damaged muscle tissue in a treated animal. The retroviral vector, by way of example, includes a dystrophin gene construct for use in treating muscular dystrophy. The present invention also comprises an experimental model utilizable in the study of the physiological regulation of skeletal muscle gene expression in intact animals.

Time- and dose-dependent effects of total-body ionizing radiation on muscle stem cells

PubMed Central

Masuda, Shinya; Hisamatsu, Tsubasa; Seko, Daiki; Urata, Yoshishige; Goto, Shinji; Li, Tao-Sheng; Ono, Yusuke

2015-01-01

Exposure to high levels of genotoxic stress, such as high-dose ionizing radiation, increases both cancer and noncancer risks. However, it remains debatable whether low-dose ionizing radiation reduces cellular function, or rather induces hormetic health benefits. Here, we investigated the effects of total-body γ-ray radiation on muscle stem cells, called satellite cells. Adult C57BL/6 mice were exposed to γ-radiation at low- to high-dose rates (low, 2 or 10 mGy/day; moderate, 50 mGy/day; high, 250 mGy/day) for 30 days. No hormetic responses in proliferation, differentiation, or self-renewal of satellite cells were observed in low-dose radiation-exposed mice at the acute phase. However, at the chronic phase, population expansion of satellite cell-derived progeny was slightly decreased in mice exposed to low-dose radiation. Taken together, low-dose ionizing irradiation may suppress satellite cell function, rather than induce hormetic health benefits, in skeletal muscle in adult mice. PMID:25869487

Regenerative fuel cell study for satellites in GEO orbit

NASA Technical Reports Server (NTRS)

Levy, Alexander; Vandine, Leslie L.; Stedman, James K.

1987-01-01

Summarized are the results of a 12-month study to identify high performance regenerative hydrogen-oxygen fuel cell concepts for geosynchronous satellite application. Emphasis was placed on concepts with the potential for high energy density (W-hr/lb) and passive means for water and heat management to maximize system reliability. Both polymer membrane and alkaline electrolyte fuel cells were considered, with emphasis on the alkaline cell because of its high performance, advanced state of development, and proven ability to operate in a launch and space environment. Three alkaline system concepts were studied. The first, the integrated design, utilized a configuration in which the fuel cell and electrolysis cells are alternately stacked inside a pressure vessel. Product water is transferred by diffusion during electrolysis and waste heat is conducted through the pressure wall, thus using completely passive means for transfer and control. The second alkaline system, the dedicated design, uses a separate fuel cell and electrolysis stack so that each unit can be optimized in size and weight based on its orbital operating period. The third design was a dual function stack configuration, in which each cell can operate in both fuel cell and electrolysis mode, thus eliminating the need for two separate stacks and associated equipment. Results indicate that using near term technology energy densities between 46 and 52 W-hr/lb can be achieved at efficiencies of 55 percent. System densities of 115 W-hr/lb are contemplated.

Age-specific functional epigenetic changes in p21 and p16 in injury-activated satellite cells

PubMed Central

Li, Ju; Han, Suhyoun; Cousin, Wendy; Conboy, Irina M.

2014-01-01

The regenerative capacity of muscle dramatically decreases with age because old muscle stem cells fail to proliferate in response to tissue damage. Here we uncover key age-specific differences underlying this proliferative decline: namely, the genetic loci of CDK inhibitors (CDKI) p21 and p16 are more epigenetically silenced in young muscle stem cells, as compared to old, both in quiescent cells and those responding to tissue injury. Interestingly, phosphorylated ERK (pERK) induced in these cells by ectopic FGF-2 is found in association with p21 and p16 promoters, and moreover, only in the old cells. Importantly, in the old satellite cells FGF-2/pERK silences p21 epigenetically and transcriptionally, which leads to reduced p21 protein levels and enhanced cell proliferation. In agreement with the epigenetic silencing of the loci, young muscle stem cells do not depend as much as old on ectopic FGF/pERK for their myogenic proliferation. In addition, other CDKIs, such asp15INK4B and p27KIP1, become elevated in satellite cells with age, confirming and explaining the profound regenerative defect of old muscle. This work enhances our understanding of tissue aging, promoting strategies for combating age-imposed tissue degeneration. PMID:25447026

Heterogeneity of adult masseter muscle satellite cells with cardiomyocyte differentiation potential.

PubMed

Huang, Wei; Liang, Jialiang; Feng, Yuliang; Jia, Zhanfeng; Jiang, Lin; Cai, Wenfeng; Paul, Christian; Gu, Jianguo G; Stambrook, Peter J; Millard, Ronald W; Zhu, Xiao-Lan; Zhu, Ping; Wang, Yigang

2018-05-26

Although resident cardiac stem cells have been reported, regeneration of functional cardiomyocytes (CMs) remains a challenge. The present study identifies an alternative progenitor source for CM regeneration without the need for genetic manipulation or invasive heart biopsy procedures. Unlike limb skeletal muscles, masseter muscles (MM) in the mouse head are developed from Nkx2-5 mesodermal progenitors. Adult masseter muscle satellite cells (MMSCs) display heterogeneity in developmental origin and cell phenotypes. The heterogeneous MMSCs that can be characterized by cell sorting based on stem cell antigen-1 (Sca1) show different lineage potential. While cardiogenic potential is preserved in Sca1 + MMSCs as shown by expression of cardiac progenitor genes (including Nkx2-5), skeletal myogenic capacity is maintained in Sca1 - MMSCs with Pax7 expression. Sca1 + MMSC-derived beating cells express cardiac genes and exhibit CM-like morphology. Electrophysiological properties of MMSC-derived CMs are demonstrated by calcium transients and action potentials. These findings show that MMSCs could serve as a novel cell source for cardiomyocyte replacement. Copyright © 2018. Published by Elsevier Inc.

Solar array experiments on the Sphinx satellite

NASA Technical Reports Server (NTRS)

Stevens, N. J.

1973-01-01

The Space Plasma, High Voltage Interaction Experiment (SPHINX) is the name given to an auxiliary payload satellite scheduled to be launched in January 1974. The principal experiments carried on this satellite are specifically designed to obtain the engineering data on the interaction of high voltage systems with the space plasma. The classes of experiments are solar array segments, insulators, insulators with pin holes and conductors. The satellite is also carrying experiments to obtain flight data on three new solar array configurations; the edge illuminated-multijunction cells, the Teflon encased cells and the violet cells.

SAC-A satellite

NASA Image and Video Library

1998-12-14

S88-E-5161 (12-14-98) --- Before beginning their pre-sleep period on their next to last day in space, the STS-88 astronauts deployed a small 590-pound satellite called SAC-A for the Argentinean National Committee of Space Activities. Equipped with five technology experiments, including one to track the movement of whales off the coast of Argentina, SAC-A was ejected from a canister in Endeavour's cargo bay at 10:31 p.m. Central time as the shuttle few over the northern Indian Ocean. The satellite is expected to remain in orbit from five to nine months sending back data to Argentine researchers back on Earth. The photo was taken with an electronic still camera (ESC) at 04:35:48 GMT, Dec. 14.

Satellite battery testing status

NASA Astrophysics Data System (ADS)

Haag, R.; Hall, S.

1986-09-01

Because of the large numbers of satellite cells currently being tested and anticipated at the Naval Weapons Support Center (NAVWPNSUPPCEN) Crane, Indiana, satellite cell testing is being integrated into the Battery Test Automation Project (BTAP). The BTAP, designed to meet the growing needs for battery testing at the NAVWPNSUPPCEN Crane, will consist of several Automated Test Stations (ATSs) which monitor batteries under test. Each ATS will interface with an Automation Network Controller (ANC) which will collect test data for reduction.

Satellite battery testing status

NASA Technical Reports Server (NTRS)

Haag, R.; Hall, S.

1986-01-01

Because of the large numbers of satellite cells currently being tested and anticipated at the Naval Weapons Support Center (NAVWPNSUPPCEN) Crane, Indiana, satellite cell testing is being integrated into the Battery Test Automation Project (BTAP). The BTAP, designed to meet the growing needs for battery testing at the NAVWPNSUPPCEN Crane, will consist of several Automated Test Stations (ATSs) which monitor batteries under test. Each ATS will interface with an Automation Network Controller (ANC) which will collect test data for reduction.

Reduced satellite cell density and myogenesis in Wagyu compared with Angus cattle as a possible explanation of its high marbling.

PubMed

Fu, X; Yang, Q; Wang, B; Zhao, J; Zhu, M; Parish, S M; Du, M

2018-05-01

Mechanisms responsible for excellent marbling in Japanese black cattle, Wagyu, remain to be established. Because both muscle cells and intramuscular adipocytes are developed from mesenchymal progenitor cells during early muscle development, we hypothesized that intramuscular progenitor cells in Wagyu cattle have attenuated myogenic capacity in favor of adipogenesis, leading to high marbling but reduced muscle growth. Biceps femoris muscle biopsy samples were obtained from both Angus (n=3) and Wagyu (n=3) cattle at 12 months of age. Compared with Angus, the density of satellite cells was much lower in Wagyu muscle (by 45.8±10%, P<0.05). Consistently, the formation of myotubes from muscle-derived progenitor cells was also lower (by 64.2±12.9%, P<0.05), but adipogenic capacity was greater in Wagyu. The average muscle fiber diameter was larger in Wagyu (by 23.9±6.8%, P=0.089) despite less muscle mass, suggesting less muscle fiber formation in Wagyu compared with Angus cattle. Because satellite cells are derived from fetal myogenic cells, the reduction in satellite cell density together with lower muscle fiber formation suggests that myogenesis was attenuated during early muscle development in Wagyu cattle. Given the shared pool of mesenchymal progenitor cells, the attenuated myogenesis likely shifts progenitor cells to adipogenesis during early development, which may contribute to high intramuscular adipocyte formation in Wagyu cattle.

An automated mapping satellite system ( Mapsat).

USGS Publications Warehouse

Colvocoresses, A.P.

1982-01-01

The favorable environment of space permits a satellite to orbit the Earth with very high stability as long as no local perturbing forces are involved. Solid-state linear-array sensors have no moving parts and create no perturbing force on the satellite. Digital data from highly stabilized stereo linear arrays are amenable to simplified processing to produce both planimetric imagery and elevation data. A satellite imaging system, called Mapsat, including this concept has been proposed to produce data from which automated mapping in near real time can be accomplished. Image maps as large as 1:50 000 scale with contours as close as a 20-m interval may be produced from Mapsat data. -from Author

Inflammatory sensitization of nociceptors depends on activation of NMDA receptors in DRG satellite cells.

PubMed

Ferrari, Luiz Fernando; Lotufo, Celina Monteiro; Araldi, Dionéia; Rodrigues, Marcos A; Macedo, Larissa P; Ferreira, Sérgio H; Parada, Carlos Amilcar

2014-12-23

The present study evaluated the role of N-methyl-D-aspartate receptors (NMDARs) expressed in the dorsal root ganglia (DRG) in the inflammatory sensitization of peripheral nociceptor terminals to mechanical stimulation. Injection of NMDA into the fifth lumbar (L5)-DRG induced hyperalgesia in the rat hind paw with a profile similar to that of intraplantar injection of prostaglandin E2 (PGE2), which was significantly attenuated by injection of the NMDAR antagonist D(-)-2-amino-5-phosphonopentanoic acid (D-AP-5) in the L5-DRG. Moreover, blockade of DRG AMPA receptors by the antagonist 6,7-dinitroquinoxaline-2,3-dione had no effect in the PGE2-induced hyperalgesia in the paw, showing specific involvement of NMDARs in this modulatory effect and suggesting that activation of NMDAR in the DRG plays an important role in the peripheral inflammatory hyperalgesia. In following experiments we observed attenuation of PGE2-induced hyperalgesia in the paw by the knockdown of NMDAR subunits NR1, NR2B, NR2D, and NR3A with antisense-oligodeoxynucleotide treatment in the DRG. Also, in vitro experiments showed that the NMDA-induced sensitization of cultured DRG neurons depends on satellite cell activation and on those same NMDAR subunits, suggesting their importance for the PGE2-induced hyperalgesia. In addition, fluorescent calcium imaging experiments in cultures of DRG cells showed induction of calcium transients by glutamate or NMDA only in satellite cells, but not in neurons. Together, the present results suggest that the mechanical inflammatory nociceptor sensitization is dependent on glutamate release at the DRG and subsequent NMDAR activation in satellite glial cells, supporting the idea that the peripheral hyperalgesia is an event modulated by a glutamatergic system in the DRG.

Business use of satellite communications

NASA Astrophysics Data System (ADS)

Edelson, B. I.; Cooper, R. S.

1982-02-01

The development, systems technology, and future applications of digital transmission systems for business use of satellite systems are explored. Intelsat currently has 22 satellites in space, applied mostly to data, telephone, and television transmission. An all digital communications network called the Integrated Services Digital Network is replacing the former, analog, systems with wideband digital transmission for voice, data, video, and industrial control information, with domestic satellites serving as the central node of transmissions in a service area. The digital systems are faster than the analog systems, and the U.S. Postal Service is developing an electronic mail system with six other countries. Various magazines are now teleprinted in various locations from broadcasts from a central source, and chain stores are employing data transmission for centralized management, inventory, and procurement.

Acute tissue injury activates satellite cells and promotes sarcoma formation via the HGF/c-MET signaling pathway.

PubMed

Van Mater, David; Añó, Leonor; Blum, Jordan M; Webster, Micah T; Huang, WeiQiao; Williams, Nerissa; Ma, Yan; Cardona, Diana M; Fan, Chen-Ming; Kirsch, David G

2015-02-01

Some patients with soft-tissue sarcoma (STS) report a history of injury at the site of their tumor. Although this phenomenon is widely reported, there are relatively few experimental systems that have directly assessed the role of injury in sarcoma formation. We recently described a mouse model of STS whereby p53 is deleted and oncogenic Kras is activated in muscle satellite cells via a Pax7(CreER) driver following intraperitoneal injection with tamoxifen. Here, we report that after systemic injection of tamoxifen, the vast majority of Pax7-expressing cells remain quiescent despite mutation of p53 and Kras. The fate of these muscle progenitors is dramatically altered by tissue injury, which leads to faster kinetics of sarcoma formation. In adult muscle, quiescent satellite cells will transition into an active state in response to hepatocyte growth factor (HGF). We show that modulating satellite cell quiescence via intramuscular injection of HGF increases the penetrance of sarcoma formation at the site of injection, which is dependent on its cognate receptor c-MET. Unexpectedly, the tumor-promoting effect of tissue injury also requires c-Met. These results reveal a mechanism by which HGF/c-MET signaling promotes tumor formation after tissue injury in a mouse model of primary STS, and they may explain why some patients develop a STS at the site of injury. ©2014 American Association for Cancer Research.

miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice.

PubMed

Zeng, Ping; Han, Wanhong; Li, Changyin; Li, Hu; Zhu, Dahai; Zhang, Yong; Liu, Xiaohong

2016-09-01

Skeletal muscle mass and homeostasis during postnatal muscle development and regeneration largely depend on adult muscle stem cells (satellite cells). We recently showed that global overexpression of miR-378 significantly reduced skeletal muscle mass in mice. In the current study, we used miR-378 transgenic (Tg) mice to assess the in vivo functional effects of miR-378 on skeletal muscle growth and regeneration. Cross-sectional analysis of skeletal muscle tissues showed that the number and size of myofibers were significantly lower in miR-378 Tg mice than in wild-type mice. Attenuated cardiotoxin-induced muscle regeneration in miR-378 Tg mice was found to be associated with delayed satellite cell activation and differentiation. Mechanistically, miR-378 was found to directly target Igf1r in muscle cells both in vitro and in vivo These miR-378 Tg mice may provide a model for investigating the physiological and pathological roles of skeletal muscle in muscle-associated diseases in humans, particularly in sarcopenia. © The Author 2016. Published by Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Fibromodulin: a master regulator of myostatin controlling progression of satellite cells through a myogenic program.

PubMed

Lee, Eun Ju; Jan, Arif Tasleem; Baig, Mohammad Hassan; Ashraf, Jalaluddin Mohammad; Nahm, Sang-Soep; Kim, Yong-Woon; Park, So-Young; Choi, Inho

2016-08-01

Differentiation of muscle satellite cells (MSCs) involves interaction of the proteins present in the extracellular matrix (ECM) with MSCs to regulate their activity, and therefore phenotype. Herein, we report fibromodulin (FMOD), a member of the proteoglycan family participating in the assembly of ECM, as a novel regulator of myostatin (MSTN) during myoblast differentiation. In addition to having a pronounced effect on the expression of myogenic marker genes [myogenin (MYOG) and myosin light chain 2 (MYL2)], FMOD was found to maintain the transcriptional activity of MSTN Moreover, coimmunoprecipitation and in silico studies performed to investigate the interaction of FMOD helped confirm that it antagonizes MSTN function by distorting its folding and preventing its binding to activin receptor type IIB. Furthermore, in vivo studies revealed that FMOD plays an active role in healing by increasing satellite cell recruitment to sites of injury. Together, these findings disclose a hitherto unrecognized regulatory role for FMOD in MSCs and highlight new mechanisms whereby FMOD circumvents the inhibitory effects of MSTN and triggers myoblast differentiation. These findings offer a basis for the design of novel MSTN inhibitors that promote muscle regeneration after injury or for the development of pharmaceutical agents for the treatment of different muscle atrophies.-Lee, E. J., Jan, A. T., Baig, M. H., Ashraf, J. M., Nahm, S.-S., Kim, Y.-W., Park, S.-Y., Choi, I. Fibromodulin: a master regulator of myostatin controlling progression of satellite cells through a myogenic program. © FASEB.

In-person contact begets calling and texting: interpersonal motives for cell phone use, face-to-face interaction, and loneliness.

PubMed

Jin, Borae; Park, Namkee

2010-12-01

This study examined how cell-phone use is related to interpersonal motives for using cell phones, face-to-face communication, and loneliness. A survey of 232 college students who owned a cell phone revealed that affection and inclusion were relatively strong motivations for using voice calls and text messaging, and that interpersonal motives were positively related to the amount of cell-phone use, including calling and texting. The amount of face-to-face interaction was positively associated with the participants' cell-phone use and their interpersonal motives for using cell phones: the more the participants engaged in face-to-face interaction with other people, the higher their motives were and the more frequent cell-phone use was. Loneliness did not have a direct relation to cell-phone use. Instead, the participants with higher levels of loneliness were less likely to engage in face-to-face social interaction, which led them to use cell phones less and to be less motivated to use cell phones for interpersonal purposes.

NASA mobile satellite program

NASA Technical Reports Server (NTRS)

Knouse, G.; Weber, W.

1985-01-01

A three phase development program for ground and space segment technologies which will enhance and enable the second and third generation mobile satellite systems (MSS) is outlined. Phase 1, called the Mobile Satellite Experiment (MSAT-X), is directed toward the development of ground segment technology needed for future MSS generations. Technology validation and preoperational experiments with other government agencies will be carried out during the two year period following launch. The satellite channel capacity needed to carry out these experiments will be obtained from industry under a barter type agreement in exchange for NASA provided launch services. Phase 2 will develop and flight test the multibeam spacecraft antenna technology needed to obtain substantial frequency reuse for second generation commercial systems. Industry will provide the antenna, and NASA will fly it on the Shuttle and test it in orbit. Phase 3 is similar to Phase 2 but will develop an even larger multibeam antenna and test it on the space station.

NASA mobile satellite program

NASA Astrophysics Data System (ADS)

Knouse, G.; Weber, W.

1985-04-01

A three phase development program for ground and space segment technologies which will enhance and enable the second and third generation mobile satellite systems (MSS) is outlined. Phase 1, called the Mobile Satellite Experiment (MSAT-X), is directed toward the development of ground segment technology needed for future MSS generations. Technology validation and preoperational experiments with other government agencies will be carried out during the two year period following launch. The satellite channel capacity needed to carry out these experiments will be obtained from industry under a barter type agreement in exchange for NASA provided launch services. Phase 2 will develop and flight test the multibeam spacecraft antenna technology needed to obtain substantial frequency reuse for second generation commercial systems. Industry will provide the antenna, and NASA will fly it on the Shuttle and test it in orbit. Phase 3 is similar to Phase 2 but will develop an even larger multibeam antenna and test it on the space station.

Space Solar Power: Satellite Concepts

NASA Technical Reports Server (NTRS)

Little, Frank E.

1999-01-01

Space Solar Power (SSP) applies broadly to the use of solar power for space related applications. The thrust of the NASA SSP initiative is to develop concepts and demonstrate technology for applying space solar power to NASA missions. Providing power from satellites in space via wireless transmission to a receiving station either on earth, another celestial body or a second satellite is one goal of the SSP initiative. The sandwich design is a satellite design in which the microwave transmitting array is the front face of a thin disk and the back of the disk is populated with solar cells, with the microwave electronics in between. The transmitter remains aimed at the earth in geostationary orbit while a system of mirrors directs sunlight to the photovoltaic cells, regardless of the satellite's orientation to the sun. The primary advantage of the sandwich design is it eliminates the need for a massive and complex electric power management and distribution system for the satellite. However, it requires a complex system for focusing sunlight onto the photovoltaic cells. In addition, positioning the photovoltaic array directly behind the transmitting array power conversion electronics will create a thermal management challenge. This project focused on developing designs and finding emerging technology to meet the challenges of solar tracking, a concentrating mirror system including materials and coatings, improved photovoltaic materials and thermal management.

Leonardo-BRDF: A New Generation Satellite Constellation

NASA Technical Reports Server (NTRS)

Esper, Jaime; Neeck, Steven; Wiscombe, Warren; Ryschkewitsch, Michael; Andary, J. (Technical Monitor)

2000-01-01

Instantaneous net radiation flux at the top of the atmosphere is one of the primary drivers of climate and global change. Since the dawn of the satellite era, great efforts and expense have gone into measuring this flux from single satellites and even (for a several-year period) from a constellation of three satellites called ERBE. However, the reflected solar flux is an angular and spectral integral over the so-called "BRDF" or Bidirectional Reflectance Distribution Function, which is the angular distribution of reflected solar radiation for each solar zenith angle and each wavelength. Previous radiation flux satellites could not measure instantaneous BRDF, so scientists have had to fall back on models or composites. Because their range of observed solar zenith angles was very limited due to sunsynchronous orbits, the resultant flux maps are too inaccurate to see the dynamics of radiation flux or to reliably correlate it with specific phenomena (hurricanes, biomass fires, urban pollution, dust outbreaks, etc.). Accuracy only becomes acceptable after monthly averaging, but this washes out almost all cause-and-effect information, further exacerbated by the lack of spectral resolution. Leonardo-BRDF is a satellite system designed to measure the instantaneous spectral BRDF using a formation of highly coordinated satellites, all pointing at the same Earth targets at the same time. It will allow scientists for the first time to assess the radiative forcing of climate due to specific phenomena, which is bound to be important in the ongoing debate about global warming and what is causing it. The formation is composed of two satellite types having, as instrument payloads, single highly-integrated miniature imaging spectrometers or radiometers. Two nearby "keystone" satellites anchor the formation and fly in static orbits. They employ wide field of view imaging spectrometers that are extremely light and compact. The keystone satellites are identical and can operate in

The role of radiation hard solar cells in minimizing the costs of global satellite communications systems

NASA Technical Reports Server (NTRS)

Summers, Geoffrey P.; Walters, Robert J.; Messenger, Scott R.; Burke, Edward A.

1995-01-01

An analysis embodied in a PC computer program is presented which quantitatively demonstrates how the availability of radiation hard solar cells can minimize the cost of a global satellite communication system. The chief distinction between the currently proposed systems, such as Iridium Odyssey and Ellipsat, is the number of satellites employed and their operating altitudes. Analysis of the major costs associated with implementing these systems shows that operation within the earth's radiation belts can reduce the total system cost by as much as a factor of two, so long as radiation hard components including solar cells, can be used. A detailed evaluation of several types of planar solar cells is given, including commercially available Si and GaAs/Ge cells, and InP/Si cells which are under development. The computer program calculates the end of life (EOL) power density of solar arrays taking into account the cell geometry, coverglass thickness, support frame, electrical interconnects, etc. The EOL power density can be determined for any altitude from low earth orbit (LEO) to geosynchronous (GEO) and for equatorial to polar planes of inclination. The mission duration can be varied over the entire range planned for the proposed satellite systems. An algorithm is included in the program for determining the degradation of cell efficiency for different cell technologies due to proton and electron irradiation. The program can be used to determine the optimum configuration for any cell technology for a particular orbit and for a specified mission life. Several examples of applying the program are presented, in which it is shown that the EOL power density of different technologies can vary by an order of magnitude for certain missions. Therefore, although a relatively radiation soft technology can be made to provide the required EOL power by simply increasing the size of the array, the impact on the total system budget could be unacceptable, due to increased launch and

Satellite Communications for ATM

NASA Technical Reports Server (NTRS)

Shamma, Mohammed A.

2003-01-01

This presentation is an overview on Satellite Communication for the Aeronautical Telecommunication Management (ATM) research. Satellite Communications are being considered by the FAA and NASA as a possible alternative to the present and future ground systems supporting Air Traffic Communications. The international Civil Aviation Organization (ICAO) have in place Standards and Recommended Practices (SARPS) for the Aeronautical Mobile Satellite Services (AMSS) which is mainly derived from the pre-existing Inmarsat service that has been in service since the 1980s. The Working Group A of the Aeronautical Mobile Communication Panel of ICAO has also been investigating SARPS for what is called the Next Generation Satellite Service (NGSS) which conforms less to the Inmarsat based architecture and explores wider options in terms of satellite architectures. Several designs are being proposed by Firms such as Boeing, ESA, NASA that are geared toward full or secondary usage of satellite communications for ATM. Satellite communications for ATM can serve several purposes ranging from primary usage where ground services would play a minimal backup role, to an integrated solution where it will be used to cover services, or areas that are less likely to be supported by the proposed and existing ground infrastructure. Such Integrated roles can include usage of satellite communications for oceanic and remote land areas for example. It also can include relieving the capacity of the ground network by providing broadcast based services of Traffic Information Services messages (TIS-B), or Flight Information Services (FIS-B) which can take a significant portion of the ground system capacity. Additionally, satellite communication can play a backup role to support any needs for ground replacement, or additional needed capacity even after the new digital systems are in place. The additional bandwidth that can be provided via satellite communications can also open the door for many new

Dynamic system simulation of small satellite projects

NASA Astrophysics Data System (ADS)

Raif, Matthias; Walter, Ulrich; Bouwmeester, Jasper

2010-11-01

A prerequisite to accomplish a system simulation is to have a system model holding all necessary project information in a centralized repository that can be accessed and edited by all parties involved. At the Institute of Astronautics of the Technische Universitaet Muenchen a modular approach for modeling and dynamic simulation of satellite systems has been developed called dynamic system simulation (DySyS). DySyS is based on the platform independent description language SysML to model a small satellite project with respect to the system composition and dynamic behavior. A library of specific building blocks and possible relations between these blocks have been developed. From this library a system model of the satellite of interest can be created. A mapping into a C++ simulation allows the creation of an executable system model with which simulations are performed to observe the dynamic behavior of the satellite. In this paper DySyS is used to model and simulate the dynamic behavior of small satellites, because small satellite projects can act as a precursor to demonstrate the feasibility of a system model since they are less complex compared to a large scale satellite project.

Robust and brilliant Raman tags based on core-satellite assemblies for brain tumor cell imaging

NASA Astrophysics Data System (ADS)

Chang, Yung-Ching; Huang, Li-Ching; Sun, Wei-Lun; Chuang, Shih Yi; Lin, Tien-Hsin; Wu, Yi-Syuan; Sze, Chun-I.; Chen, Shiuan-Yeh

2018-02-01

GBM (Glioblastoma Multiforme), a fatal brain tumor, is highly infiltrative and difficult to be completely removed by the surgery. In this work, the Raman tags based on the plasmonic core-satellite assemblies with 1 nm internal gap accompanied by extremely high gap field have been fabricated and applied to GBM cell labeling. The brightness of the Raman tags is comparable to the fluorophores. The GBM cells with overexpression of EGFR are labeled with these Raman tags and can be distinguished from the normal cells through Raman imaging.

Solar power satellite, system definition study. Part 2, volume 3: SPS satellite systems

NASA Technical Reports Server (NTRS)

1977-01-01

The differences in approach to solar energy conversion by solar cells and thermal engine systems are examined. Systems requirements for the solar power satellite (SPS) are given along with a description of the primary subsystems. Trades leading to exact configuration selection, for example, selection of the Rankine cycle operating temperatures are explained, and two satellite configurations are discussed.

Long-term insulin-like growth factor-I expression in skeletal muscles attenuates the enhanced in vitro proliferation ability of the resident satellite cells in transgenic mice

NASA Technical Reports Server (NTRS)

Chakravarthy, M. V.; Fiorotto, M. L.; Schwartz, R. J.; Booth, F. W.

2001-01-01

Insulin-like growth factor-I (IGF-I) overexpression for 1-month in mouse skeletal muscle increases satellite cell proliferation potential. However, it is unknown whether this beneficial enhancement by IGF-I expression would persist over a longer-term duration in aged mice. This is an important issue to address if a prolonged course of IGF-I is to be used clinically in muscle-wasting conditions where satellite cells may become limiting. Using the IGF-I transgenic (IGF-I Tg) mouse that selectively expresses the IGF-I transgene in striated muscles, we found that 18-months of continuous IGF-I overexpression led to a loss in the enhanced in vitro proliferative capacity of satellite cells from Tg skeletal muscles. Also 18-month-old IGF-I Tg satellite cells lost the enhanced BrdU incorporation, greater pRb and Akt phosphorylations, and decreased p27(Kip1) levels initially observed in cells from 1-month-old IGF-I Tg mice. The levels of those biochemical markers reverted to similar values seen in the 18-months WT littermates. These findings, therefore, suggest that there is no further beneficial effect on enhancing satellite cell proliferation ability with persistent long-term expression of IGF-I in skeletal muscles of these transgenic mice.

Satellite-Based EMI Detection, Identification, and Mitigation

NASA Astrophysics Data System (ADS)

Stottler, R.; Bowman, C.

2016-09-01

Commanding, controlling, and maintaining the health of satellites requires a clear operating spectrum for communications. Electro Magnetic Interference (EMI) from other satellites can interfere with these communications. Determining which satellite is at fault improves space situational awareness and can be used to avoid the problem in the future. The Rfi detection And Prediction Tool, Optimizing Resources (RAPTOR) monitors the satellite communication antenna signals to detect EMI (also called RFI for Radio Frequency Interference) using a neural network trained on past cases of both normal communications and EMI events. RAPTOR maintains a database of satellites that have violated the reserved spectrum in the past. When satellite-based EMI is detected, RAPTOR first checks this list to determine if any are angularly close to the satellite being communicated with. Additionally, RAPTOR checks the Space Catalog to see if any of its active satellites are angularly close. RAPTOR also consults on-line databases to determine if the described operating frequencies of the satellites match the detected EMI and recommends candidates to be added to the known offenders database, accordingly. Based on detected EMI and predicted orbits and frequencies, RAPTOR automatically reschedules satellite communications to avoid current and future satellite-based EMI. It also includes an intuitive display for a global network of satellite communications antennas and their statuses including the status of their EM spectrum. RAPTOR has been prototyped and tested with real data (amplitudes versus frequency over time) for both satellite communication signals and is currently undergoing full-scale development. This paper describes the RAPTOR technologies and results of testing.

Satellite freeze forecast system: Executive summary

NASA Technical Reports Server (NTRS)

Martsolf, J. D. (Principal Investigator)

1983-01-01

A satellite-based temperature monitoring and prediction system consisting of a computer controlled acquisition, processing, and display system and the ten automated weather stations called by that computer was developed and transferred to the national weather service. This satellite freeze forecasting system (SFFS) acquires satellite data from either one of two sources, surface data from 10 sites, displays the observed data in the form of color-coded thermal maps and in tables of automated weather station temperatures, computes predicted thermal maps when requested and displays such maps either automatically or manually, archives the data acquired, and makes comparisons with historical data. Except for the last function, SFFS handles these tasks in a highly automated fashion if the user so directs. The predicted thermal maps are the result of two models, one a physical energy budget of the soil and atmosphere interface and the other a statistical relationship between the sites at which the physical model predicts temperatures and each of the pixels of the satellite thermal map.

Estimating the Earth's gravity field using a multi-satellite SLR solution

NASA Astrophysics Data System (ADS)

Bloßfeld, Mathis; Stefka, Vojtech; Müller, Horst; Gerstl, Michael

2013-04-01

Satellite Laser Ranging (SLR) is the unique technique to determine station coordinates, Earth Orientation Parameter (EOP) and Stokes coefficients of the Earth's gravity field in one common adjustment. These parameters form the so called "three pillars" (Plag & Pearlman, 2009) of the Global Geodetic Observing System (GGOS). In its function as official analysis center of the International Laser Ranging Service (ILRS), DGFI is developing and maintaining software to process SLR observations called "DGFI Orbit and Geodetic parameter estimation Software" (DOGS). The software is used to analyze SLR observations and to compute multi-satellite solutions. To take benefit of different orbit performances (e.g. inclination and altitude), a solution using ten different spherical satellites (ETALON1/2, LAGEOS1/2, STELLA, STARLETTE, AJISAI, LARETS, LARES, BLITS) covering 12 years of observations is computed. The satellites are relatively weighted using a variance component estimation (VCE). The obtained weights are analyzed w.r.t. the potential of the satellite to monitor changes in the Earths geometry, rotation and gravity field. The estimated parameters (station coordinates and EOP) are validated w.r.t. official time series of the IERS. The obtained Stokes coefficients are compared to recent gravity field solutions and discussed in detail.

The data of establishing a three-dimensional culture system for in vitro recapitulation and mechanism exploration of tumor satellite formation during cancer cell transition.

PubMed

Chen, Chun-Nan; Chen, You-Tzung; Yang, Tsung-Lin

2017-12-01

Tumor satellite formation is an indicator of cancer invasiveness and correlates with recurrence, metastasis, and poorer prognosis. By analyzing pathological specimens, tumor satellites formed at the tumor-host interface reflect the phenomena of epithelial-mesenchymal transition. It is impossible to reveal the dynamic processes and the decisive factors of tumor satellite formation using clinicopathological approaches alone. Therefore, establishment of an in vitro system to monitor the phenomena is important to explicitly elucidate underlying mechanisms. In this study, we explored the feasibility of creating an in vitro three-dimensional collagen culture system to recapitulate the process of tumor satellite formation. This data presented here are referred to the research article (Chen et al., 2017) [1]. Using this model, the dynamic process of tumor satellite formation could be recapitulated in different types of human cancer cells. Induced by calcium deprivation, the treated cells increased the incidence and migratory distance of tumor satellites. E-cadherin internalization and invadopodia formation were enhanced by calcium deprivation and were associated with cellular dynamic change during tumor satellite formation. The data confirmed the utility of this culture system to recapitulate dynamic cellular alteration and to explore the potential mechanisms of tumor satellite formation.

Astronaut Dale Gardner using MMU to travel to Westar VI satellite

NASA Technical Reports Server (NTRS)

1984-01-01

Astronaut Dale A. Gardner, wearing the manned maneuvering unit (MMU) approaching the spinng Westar VI satellite over Bahama Banks. Gardner uses a large tool called the apogee kick motor capture device (ACD) to enter the nozzle of the spent Westar engine and stabilize the satellite to capture it for return to Earth.

A Future of Satellite-Aided Search and Rescue

NASA Technical Reports Server (NTRS)

Wallace, Ronald

1998-01-01

Satellite technology has been an integral part of maritime search and rescue since the Cospas-Sarsat system began operation in 1984. This system, credited with more than eighty-six hundred lives saved, has recently been augmented to provide immediate response through geostationary satellites. The other satellite-based distress alerting system, INMARSAT, launched its emergency Standard C service in 1991 and Standard E in 1997. Current plans call for a continuation of service from both of these vital systems at least through the first decade of the next century. We are currently witnessing the construction of a number of new satellite systems that will have the potential for revolutionizing mobile communications. These systems will be capable of emergency communication, and must be given due consideration in any look at the future, This paper reviews existing systems using satellites for distress alerting, describes the plans in place for them, and discusses likely developments.

Satellite cell senescence underlies myopathy in a mouse model of limb-girdle muscular dystrophy 2H

PubMed Central

Kudryashova, Elena; Kramerova, Irina; Spencer, Melissa J.

2012-01-01

Mutations in the E3 ubiquitin ligase tripartite motif-containing 32 (TRIM32) are responsible for the disease limb-girdle muscular dystrophy 2H (LGMD2H). Previously, we generated Trim32 knockout mice (Trim32–/– mice) and showed that they display a myopathic phenotype accompanied by neurogenic features. Here, we used these mice to investigate the muscle-specific defects arising from the absence of TRIM32, which underlie the myopathic phenotype. Using 2 models of induced atrophy, we showed that TRIM32 is dispensable for muscle atrophy. Conversely, TRIM32 was necessary for muscle regrowth after atrophy. Furthermore, TRIM32-deficient primary myoblasts underwent premature senescence and impaired myogenesis due to accumulation of PIAS4, an E3 SUMO ligase and TRIM32 substrate that was previously shown to be associated with senescence. Premature senescence of myoblasts was also observed in vivo in an atrophy/regrowth model. Trim32–/– muscles had substantially fewer activated satellite cells, increased PIAS4 levels, and growth failure compared with wild-type muscles. Moreover, Trim32–/– muscles exhibited features of premature sarcopenia, such as selective type II fast fiber atrophy. These results imply that premature senescence of muscle satellite cells is an underlying pathogenic feature of LGMD2H and reveal what we believe to be a new mechanism of muscular dystrophy associated with reductions in available satellite cells and premature sarcopenia. PMID:22505452

Skeletal muscle wasting with disuse atrophy is multi-dimensional: the response and interaction of myonuclei, satellite cells and signaling pathways

PubMed Central

Brooks, Naomi E.; Myburgh, Kathryn H.

2014-01-01

Maintenance of skeletal muscle is essential for health and survival. There are marked losses of skeletal muscle mass as well as strength and physiological function under conditions of low mechanical load, such as space flight, as well as ground based models such as bed rest, immobilization, disuse, and various animal models. Disuse atrophy is caused by mechanical unloading of muscle and this leads to reduced muscle mass without fiber attrition. Skeletal muscle stem cells (satellite cells) and myonuclei are integrally involved in skeletal muscle responses to environmental changes that induce atrophy. Myonuclear domain size is influenced differently in fast and slow twitch muscle, but also by different models of muscle wasting, a factor that is not yet understood. Although the myonuclear domain is 3-dimensional this is rarely considered. Apoptosis as a mechanism for myonuclear loss with atrophy is controversial, whereas cell death of satellite cells has not been considered. Molecular signals such as myostatin/SMAD pathway, MAFbx, and MuRF1 E3 ligases of the ubiquitin proteasome pathway and IGF1-AKT-mTOR pathway are 3 distinctly different contributors to skeletal muscle protein adaptation to disuse. Molecular signaling pathways activated in muscle fibers by disuse are rarely considered within satellite cells themselves despite similar exposure to unloading or low mechanical load. These molecular pathways interact with each other during atrophy and also when various interventions are applied that could alleviate atrophy. Re-applying mechanical load is an obvious method to restore muscle mass, however how nutrient supplementation (e.g., amino acids) may further enhance recovery (or reduce atrophy despite unloading or ageing) is currently of great interest. Satellite cells are particularly responsive to myostatin and to growth factors. Recently, the hibernating squirrel has been identified as an innovative model to study resistance to atrophy. PMID:24672488

Preclinical characterization of the JAK/STAT inhibitor SGI-1252 on skeletal muscle function, morphology, and satellite cell content.

PubMed

Sorensen, Jacob R; Fuqua, Jordan D; Deyhle, Michael R; Parmley, Jacob; Skousen, Caitlin; Hancock, Chad; Parcell, Allen C; Hyldahl, Robert D

2018-01-01

Recent studies have highlighted the JAK/STAT signaling pathway in the regulation of muscle satellite cell behavior. Herein we report preclinical studies designed to characterize the effects of a novel JAK/STAT inhibitor on plantar flexor skeletal muscle function, morphology, and satellite cell content. The compound, SGI-1252, was administered orally (400mg/kg) in a 10% dextrose solution to wild type mice (n = 6) 3 times per week for 8 weeks. A control group (n = 6) received only the dextrose solution. SGI-1252 was well tolerated, as animals displayed similar weight gain over the 8-week treatment period. Following treatment, fatigue in the gastrocnemius-soleus-plantaris complex was greater in the SGI-1252 mice during a 300 second tetanic contraction bout (p = 0.035), though both the rate of fatigue and maximal force production were similar. SGI-1252 treated mice had increased type II myofiber cross-sectional area (1434.8 ± 225.4 vs 1754.7 ± 138.5 μm2), along with an increase in wet muscle mass (125.45 ± 5.46 vs 139.6 ± 12.34 mg, p = 0.032) of the gastrocnemius relative to vehicle treated mice. SGI-1252 treatment reduced gastrocnemius STAT3 phosphorylation 53% (94.79 ± 45.9 vs 44.5 ± 6.1 MFI) and significantly increased the concentration of Pax7+ satellite cells (2589.2 ± 105.5 vs 2859.4 ± 177.5 SC/mm3) in the gastrocnemius. SGI-1252 treatment suppressed MyoD (p = 0.013) and Myogenin (p<0.0001) expression in human primary myoblasts, resulting in reduced myogenic differentiation (p = 0.039). Orally delivered SGI-1252 was well tolerated, attenuates skeletal muscle STAT3 activity, and increases satellite cell content in mouse gastrocnemius muscle, likely by inhibiting myogenic progression.

A conserved function for pericentromeric satellite DNA

PubMed Central

Jagannathan, Madhav; Cummings, Ryan

2018-01-01

A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarded as junk, is a critical constituent of the chromosome, allowing the packaging of all chromosomes into a single nucleus. We show that the multi-AT-hook satellite DNA-binding proteins, Drosophila melanogaster D1 and mouse HMGA1, play an evolutionarily conserved role in bundling pericentromeric satellite DNA from heterologous chromosomes into ‘chromocenters’, a cytological association of pericentromeric heterochromatin. Defective chromocenter formation leads to micronuclei formation due to budding from the interphase nucleus, DNA damage and cell death. We propose that chromocenter and satellite DNA serve a fundamental role in encapsulating the full complement of the genome within a single nucleus, the universal characteristic of eukaryotic cells. PMID:29578410

Thermospheric density estimation from SLR observations of LEO satellites - A case study with the ANDE-Pollux satellite

NASA Astrophysics Data System (ADS)

Blossfeld, M.; Schmidt, M.; Erdogan, E.

2016-12-01

The thermospheric neutral density plays a crucial role within the equation of motion of Earth orbiting objects since drag, lift or side forces are one of the largest non-gravitational perturbations acting on the satellite. Precise Orbit Determination (POD) methods can be used to estimate thermospheric density variations from measured orbit determinations. One method which provides highly accurate measurements of the satellite position is Satellite Laser Ranging (SLR). Within the POD process, scaling factors are estimated frequently. These scaling factors can be either used for the scaling of the so called satellite-specific drag (ballistic) coefficients or the integrated thermospheric neutral density. We present a method for analytically model the drag coefficient based on a couple of physical assumptions and key parameters. In this paper, we investigate the possibility to use SLR observations to the very low Earth orbiting satellite ANDE-Pollux (approximately at 350km altitude) to determine scaling factors for different a priori thermospheric density models. We perform a POD for ANDE-Pollux covering 49 days between August 2009 and September 2009 which means the time span containing the largest number of observations during the short lifetime of the satellite. Finally, we compare the obtained scaled thermospheric densities w.r.t. each other

Effect of 8-week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats.

PubMed

Lim, Chang Hyun; Gil, Ju Hyun; Quan, Helong; Viet, Dang Ha; Kim, Chang Keun

2018-06-01

We investigated the effects of regular leucine intake and/or resistance exercise training on skeletal muscle hypertrophy and satellite cell activity after the administration of different doses of leucine. Ten-week-old Sprague-Dawley rats were assigned to six groups (n = 7 per group): a control group (Con), two groups receiving either 10% (0.135 g/kg.wt) (Leu10) or 50% (0.675 g/kg.wt) (Leu50) leucine supplementation, and three exercise groups receiving 0% (Ex), 10% (Leu10Ex), and 50% (Leu50Ex) leucine supplementation. The rats performed ladder climbing exercises thrice per week for 8 weeks, and received leucine supplements at the same time daily. Muscle phenotypes were assessed by immunohistochemistry. MyoD, myogenin, and IGF1 protein levels were determined by western blot. The Leu50Ex group displayed significantly higher numbers of positive embryonic myosin fibers (0.35 ± 0.08, 250%) and myonuclei (3.29 ± 0.3, 118.7%) than all other groups. And exercise training groups increased the cross-sectional area, the number of satellite cells and protein expression of MyoD, myogenin, and IGF1alpha relative to the Control group (P < 0.05). However, Only leucine supplementation group did not increase skeletal muscle hypertrophy and satellite cell activity, regardless of the dose (P > 0.05). Leucine intake accompanied by regular exercise training may increase satellite cell activation in skeletal muscles, and improve muscle quality more effectively than continuous leucine ingestion alone. © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

Limitations imposed by ionospheric turbulence on satellite-to-satellite Doppler measurement accuracy. [of earth gravity field

NASA Technical Reports Server (NTRS)

Grossi, M. D.

1982-01-01

For some time the possibility has been considered to perform an accurate survey from orbit of the earth gravity field by making use of low-low, satellite-to-satellite Doppler tracking with a radio link which operates in the frequency band in the range from 50 to 100 GHz. It is, therefore, of interest to discuss the upper bound in Doppler measurement accuracy imposed by the effects of ionospheric turbulence. The present investigation is concerned with the measurement error induced by ionospheric turbulence. The assumptin is made that the so-called ionospheric refractive 'bias' can be removed with one of the multifrequency methods of the current practice.

The Advanced Communication Technology Satellite and ISDN

NASA Technical Reports Server (NTRS)

Lowry, Peter A.

1996-01-01

This paper depicts the Advanced Communication Technology Satellite (ACTS) system as a global central office switch. The ground portion of the system is the collection of earth stations or T1-VSAT's (T1 very small aperture terminals). The control software for the T1-VSAT's resides in a single CPU. The software consists of two modules, the modem manager and the call manager. The modem manager (MM) controls the RF modem portion of the T1-VSAT. It processes the orderwires from the satellite or from signaling generated by the call manager (CM). The CM controls the Recom Laboratories MSPs by receiving signaling messages from the stacked MSP shelves ro units and sending appropriate setup commands to them. There are two methods used to setup and process calls in the CM; first by dialing up a circuit using a standard telephone handset or, secondly by using an external processor connected to the CPU's second COM port, by sending and receiving signaling orderwires. It is the use of the external processor which permits the ISDN (Integrated Services Digital Network) Signaling Processor to implement ISDN calls. In August 1993, the initial testing of the ISDN Signaling Processor was carried out at ACTS System Test at Lockheed Marietta, Princeton, NJ using the spacecraft in its test configuration on the ground.

Muscle regeneration potential and satellite cell activation profile during recovery following hindlimb immobilization in mice.

PubMed

Guitart, Maria; Lloreta, Josep; Mañas-Garcia, Laura; Barreiro, Esther

2018-05-01

Reduced muscle activity leads to muscle atrophy and function loss in patients and animal models. Satellite cells (SCs) are postnatal muscle stem cells that play a pivotal role in skeletal muscle regeneration following injury. The regenerative potential, satellite cell numbers, and markers during recovery following immobilization of the hindlimb for 7 days were explored. In mice exposed to 7 days of hindlimb immobilization, in those exposed to recovery (7 days, splint removal), and in contralateral control muscles, muscle precursor cells were isolated from all hindlimb muscles (fluorescence-activated cell sorting, FACS) and SCs, and muscle regeneration were identified using immunofluorescence (gastrocnemius and soleus) and electron microscopy (EM, gastrocnemius). Expression of ki67, pax7, myoD, and myogenin was quantified (RT-PCR) from SC FACS yields. Body and grip strength were determined. Following 7 day hindlimb immobilization, a decline in SCs (FACS, immunofluorescence) was observed together with an upregulation of SC activation markers and signs of muscle regeneration including fusion to existing myofibers (EM). Recovery following hindlimb immobilization was characterized by a program of muscle regeneration events. Hindlimb immobilization induced a decline in SCs together with an upregulation of markers of SC activation, suggesting that fusion to existing myofibers takes place during unloading. Muscle recovery induced a significant rise in muscle precursor cells and regeneration events along with reduced SC activation expression markers and a concomitant rise in terminal muscle differentiation expression. These are novel findings of potential applicability for the treatment of disuse muscle atrophy, which is commonly associated with severe chronic and acute conditions. © 2017 Wiley Periodicals, Inc.

Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise.

PubMed

Reidy, Paul T; Fry, Christopher S; Dickinson, Jared M; Drummond, Micah J; Rasmussen, Blake B

2017-06-01

Aged skeletal muscle has an attenuated and delayed ability to proliferate satellite cells in response to resistance exercise. The mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway is a focal point for cell growth, however, the effect of postexercise mTORC1 activation on human skeletal muscle satellite cell (SC) proliferation is unknown. To test the proliferative capacity of skeletal muscle SC in aging muscle to a potent mTORC1 activator (i.e., EAA; essential amino acids) we recruited older (~72y) men to conduct leg resistance exercise (8setsx10reps) without (-EAA; n  = 8) and with (+EAA: n  = 11) ingestion of 10 g of EAA 1 h postexercise. Muscle biopsies were taken before exercise (Pre) and 24 h postexercise (Post) for assessment of expression and fiber type-specific Pax7 + SC, Ki67 + Pax7 + SC and MyoD + SC -EAA did not show an increase in Pax7 + satellite cells at Post( P  > 0.82). Although statistical significance for an increase in Pax7 +  SC at 24 h post-RE was not observed in +EAA versus -EAA, we observed trends for a treatment difference ( P  < 0.1). When examining the change from Pre to Post trends were demonstrated (#/myofiber: P  = 0.076; and %/myonuclei: P  = 0.065) for a greater increase in +EAA versus -EAA Notably, we found an increase SC proliferation in +EAA, but not -EAA with increase in Ki67 + SC and MyoD + cells ( P  < 0.05). Ki67 + SC also exhibited a significant group difference Post ( P  < 0.010). Pax7 + SC in fast twitch myofibers did not change and were not different between groups ( P  > 0.10). CDK2, MEF2C, RB1 mRNA only increased in +EAA ( P  < 0.05). Acute muscle satellite cell proliferative capacity may be partially rescued with postexercise EAA ingestion in older men. © 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

Improved Assimilation of Streamflow and Satellite Soil Moisture with the Evolutionary Particle Filter and Geostatistical Modeling

NASA Astrophysics Data System (ADS)

Yan, Hongxiang; Moradkhani, Hamid; Abbaszadeh, Peyman

2017-04-01

Assimilation of satellite soil moisture and streamflow data into hydrologic models using has received increasing attention over the past few years. Currently, these observations are increasingly used to improve the model streamflow and soil moisture predictions. However, the performance of this land data assimilation (DA) system still suffers from two limitations: 1) satellite data scarcity and quality; and 2) particle weight degeneration. In order to overcome these two limitations, we propose two possible solutions in this study. First, the general Gaussian geostatistical approach is proposed to overcome the limitation in the space/time resolution of satellite soil moisture products thus improving their accuracy at uncovered/biased grid cells. Secondly, an evolutionary PF approach based on Genetic Algorithm (GA) and Markov Chain Monte Carlo (MCMC), the so-called EPF-MCMC, is developed to further reduce weight degeneration and improve the robustness of the land DA system. This study provides a detailed analysis of the joint and separate assimilation of streamflow and satellite soil moisture into a distributed Sacramento Soil Moisture Accounting (SAC-SMA) model, with the use of recently developed EPF-MCMC and the general Gaussian geostatistical approach. Performance is assessed over several basins in the USA selected from Model Parameter Estimation Experiment (MOPEX) and located in different climate regions. The results indicate that: 1) the general Gaussian approach can predict the soil moisture at uncovered grid cells within the expected satellite data quality threshold; 2) assimilation of satellite soil moisture inferred from the general Gaussian model can significantly improve the soil moisture predictions; and 3) in terms of both deterministic and probabilistic measures, the EPF-MCMC can achieve better streamflow predictions. These results recommend that the geostatistical model is a helpful tool to aid the remote sensing technique and the EPF-MCMC is a

Gliopathic Pain: When Satellite Glial Cells Go Bad

PubMed Central

Ohara, Peter T.; Vit, Jean-Philippe; Bhargava, Aditi; Romero, Marcela; Sundberg, Christopher; Charles, Andrew C.; Jasmin, Luc

2010-01-01

Neurons in sensory ganglia are surrounded by satellite glial cells (SGCs) that perform similar functions to the glia found in the CNS. When primary sensory neurons are injured, the surrounding SGCs undergo characteristic changes. There is good evidence that the SGCs are not just bystanders to the injury but play an active role in the initiation and maintenance of neuronal changes that underlie neuropathic pain. In this article the authors review the literature on the relationship between SGCs and nociception and present evidence that changes in SGC potassium ion buffering capacity and glutamate recycling can lead to neuropathic pain-like behavior in animal models. The role that SGCs play in the immune responses to injury is also considered. We propose the term gliopathic pain to describe those conditions in which central or peripheral glia are thought to be the principal generators of principal pain generators. PMID:19826169

Commercial Satellite Imagery Analysis for Countering Nuclear Proliferation

NASA Astrophysics Data System (ADS)

Albright, David; Burkhard, Sarah; Lach, Allison

2018-05-01

High-resolution commercial satellite imagery from a growing number of private satellite companies allows nongovernmental analysts to better understand secret or opaque nuclear programs of countries in unstable or tense regions, called proliferant states. They include North Korea, Iran, India, Pakistan, and Israel. By using imagery to make these countries’ aims and capabilities more transparent, nongovernmental groups like the Institute for Science and International Security have affected the policies of governments and the course of public debate. Satellite imagery work has also strengthened the efforts of the International Atomic Energy Agency, thereby helping this key international agency build its case to mount inspections of suspect sites and activities. This work has improved assessments of the nuclear capabilities of proliferant states. Several case studies provide insight into the use of commercial satellite imagery as a key tool to educate policy makers and affect policy.

Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals.

PubMed

Pugh, Jamie K; Faulkner, Steve H; Turner, Mark C; Nimmo, Myra A

2018-02-01

Sarcopenia can begin from the 4-5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone. Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HR max on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7 + cells) and active (MyoD + cells) satellite cell number using immunofluorescence microscopy. Type-I-specific Pax7 + (P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD + (P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7 + and MyoD + cell number remained unchanged after both exercise trials (all P ≥ 0.13). Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middle-aged individuals.

A satellite system for land-mobile communications in Europe

NASA Technical Reports Server (NTRS)

Bartholome, P.; Rogard, R.

1988-01-01

There exists a great unsatisified demand for land mobile communications in Europe, particularly in sectors of business activity such as the road transport industry. This demand could best be satisfied by means of satellite-based private networks providing voice and data communications in a hub configuration. The potential market is estimated to encompass several hundred thousand road vehicles and the transmission capacity required would be several thousand channels. ESA is currently demonstrating the potential of satellite communications for this type of application, using a system called PRODAT. System studies are being performed with the aim of defining the architecture of a regional satellite system for Europe.

Satellites at Work, Space in the Seventies.

ERIC Educational Resources Information Center

Corliss, William R.

This publication in the "Space in the Seventies" series describes current status and future plans for "working" spacecraft, also called "application satellites." These spacecraft serve the needs of communications, meteorology, geodesy, and navigation. They also enable us to study earth resources from space. Many scientific and technical concepts…

Healthy satellites provide quality service

NASA Astrophysics Data System (ADS)

Margittai, Paul

The procedures used by Telesat Canada to ensure the performance of Anik satellites is described. At the Satellite Telemetry, Tracking and Command station, each Anik satellite has its own dedicated antenna. Telemetered information from the satellites is received, processed and forwarded to the Satellite Control Center. There the satellite controllers issue all commands to the satellites, and continually monitor the telemetered data. These data, describing the state of health of the satellites, are then analyzed by engineering specialists. The satellites are held in their precise orbital positions by means of specially developed software. The Anik C and D satellites employ travelling wave tube amplifiers (TWTAs) in the transpounder channels. The TWTA saturated flux density (SFD) and equivalent isotropic radiated power (EIRP) are regularly measured and their trends scrutinized. This ensures that customers receive the radio frequency power needed for high-quality service. The satellite electrical power is supplied by the solar cell array and batteries. Power system performance is evaluated regularly to ensure that power is available to operate the required number of TWTAs. In addition to rain-fades, short service interruptions can be caused by high voltage trip-offs of TWTAs, and by electrostatic discharge related anomalies. To minimize these interruptions, Telesat ensures that the satellite controllers are fully trained and prepared for any eventuality, and the relevant operational procedures are continually refined. A fully trained staff of satellite controllers keep interruptions caused by high voltage trip-offs of TWTAs and by electrostatic discharge to a minimum.

The effect of nutritional status on myogenic satellite cell proliferation and differentiation.

PubMed

Powell, D J; McFarland, D C; Cowieson, A J; Muir, W I; Velleman, S G

2013-08-01

Early posthatch satellite cell (SC) mitotic activity is a critical component of muscle development and growth. Satellite cells are stem cells that can be induced by nutrition to follow other cellular developmental pathways. The objective of the current study was to determine the effect of restricting protein synthesis on the proliferation and differentiation of SC, using variable concentrations of Met and Cys to modulate protein synthesis. Broiler pectoralis major SC were cultured and treated with 1 of 6 different Met/Cys concentrations: 60/192, 30/96 (control), 7.5/24, 3/9.6, 1/3.2, or 0/0 mg/L. The effect of Met/Cys concentration on SC proliferation and differentiation was measured, and myonuclear accretion was measured by counting the number of nuclei per myotube during differentiation. The 30/96 mg/L Met/Cys treatment resulted in the highest rate of proliferation compared with all other treatments by 72 h of proliferation (P < 0.05). Differentiation was measured with Met/Cys treatments only during proliferation and the cultures receiving normal differentiation medium (R/N), normal proliferation medium and differentiation medium with variable Met/Cys (N/R), or both proliferation and differentiation receiving variable Met/Cys treatments (R/R). Differentiation responded in a dose-dependent manner to Met/Cys concentration under all 3 of these treatment regimens, with a degree of recovery in the R/N regimen cells following reinstatement of the control medium. Reductions in both proliferation and differentiation were more pronounced as Met/Cys concentrations were further reduced, whereas increased differentiation was observed under the increased Met/Cys concentration treatment when applied during differentiation in the N/R and R/R regimens. The number of nuclei per myotube was significantly decreased in the severely Met/Cys restricted treatments (P < 0.05). These data demonstrate the sensitivity of pectoralis major SC to nutritional availability and the importance of

Satellite-based Flood Modeling Using TRMM-based Rainfall Products.

PubMed

Harris, Amanda; Rahman, Sayma; Hossain, Faisal; Yarborough, Lance; Bagtzoglou, Amvrossios C; Easson, Greg

2007-12-20

Increasingly available and a virtually uninterrupted supply of satellite-estimatedrainfall data is gradually becoming a cost-effective source of input for flood predictionunder a variety of circumstances. However, most real-time and quasi-global satelliterainfall products are currently available at spatial scales ranging from 0.25 o to 0.50 o andhence, are considered somewhat coarse for dynamic hydrologic modeling of basin-scaleflood events. This study assesses the question: what are the hydrologic implications ofuncertainty of satellite rainfall data at the coarse scale? We investigated this question onthe 970 km² Upper Cumberland river basin of Kentucky. The satellite rainfall productassessed was NASA's Tropical Rainfall Measuring Mission (TRMM) Multi-satellitePrecipitation Analysis (TMPA) product called 3B41RT that is available in pseudo real timewith a latency of 6-10 hours. We observed that bias adjustment of satellite rainfall data canimprove application in flood prediction to some extent with the trade-off of more falsealarms in peak flow. However, a more rational and regime-based adjustment procedureneeds to be identified before the use of satellite data can be institutionalized among floodmodelers.

Non-Geo systems—where have all the satellites gone?

NASA Astrophysics Data System (ADS)

Ashford, Edward W.

2004-08-01

Non-GEO satellites were being hailed as the means that would bring connectivity to the masses. Anyone, it was said, would be able to buy a portable telephone that would allow them, no matter where they might be, to call or be called by anyone else in the world. In the early 1990s, there were predictions that, by now, literally many hundreds of new non-geostationary communications satellites would be in orbit. Furthermore, people all over the world would also be able to buy an inexpensive satellite terminal that would allow them to access the Internet with a broadband connection, at prices on par with or even far below what the terrestrial competition would be able to offer. Many companies were set up to compete to establish this "Nirvana of Communications." To do so, however, the companies in question would have to attract sufficient financial backing from investors to actually begin such an undertaking. Surprisingly, at least in retrospect, a tremendous amount of investment capital was forthcoming, and a number of contracts were awarded to manufacturers to develop the requisite satellite systems. Today, almost a decade later, satellite manufacturers and their suppliers have been subjected to a massive belt-tightening exercise as contracts were cancelled and expected future orders were not forthcoming. The launch vehicle industry, which had geared up to meet the bonanza of expected launch orders, is also going hungry. Where are all the proposed systems? This paper will attempt to answer this question, as well as to provide some insight into why the systems that are no longer with us fell by the wayside. Finally, it will look into the future and try to predict what the long-term prospects might be for non-GEO communication satellites in general.

Satellite-based Flood Modeling Using TRMM-based Rainfall Products

PubMed Central

Harris, Amanda; Rahman, Sayma; Hossain, Faisal; Yarborough, Lance; Bagtzoglou, Amvrossios C.; Easson, Greg

2007-01-01

Increasingly available and a virtually uninterrupted supply of satellite-estimated rainfall data is gradually becoming a cost-effective source of input for flood prediction under a variety of circumstances. However, most real-time and quasi-global satellite rainfall products are currently available at spatial scales ranging from 0.25° to 0.50° and hence, are considered somewhat coarse for dynamic hydrologic modeling of basin-scale flood events. This study assesses the question: what are the hydrologic implications of uncertainty of satellite rainfall data at the coarse scale? We investigated this question on the 970 km2 Upper Cumberland river basin of Kentucky. The satellite rainfall product assessed was NASA's Tropical Rainfall Measuring Mission (TRMM) Multi-satellite Precipitation Analysis (TMPA) product called 3B41RT that is available in pseudo real time with a latency of 6-10 hours. We observed that bias adjustment of satellite rainfall data can improve application in flood prediction to some extent with the trade-off of more false alarms in peak flow. However, a more rational and regime-based adjustment procedure needs to be identified before the use of satellite data can be institutionalized among flood modelers. PMID:28903302

Satellite Catalog Renumbering: What Does That Mean and Should I Be Worried?

DTIC Science & Technology

2013-04-02

Line Element Set ". Because of growth of the number of objects in the satellite catalog, it is expected that the fixed format will be insufficient to...the "Two Line Element Set ". Because of growth of the number of objects in the satellite catalog, it is expected that the fixed format will be...on what is called a "Two Line Element Set " (TLE). Using this data in the proper formulas allows us to predict when the satellite will be overhead and

Autonomous sensor-based dual-arm satellite grappling

NASA Technical Reports Server (NTRS)

Wilcox, Brian; Tso, Kam; Litwin, Todd; Hayati, Samad; Bon, Bruce

1989-01-01

Dual-arm satellite grappling involves the integration of technologies developed in the Sensing and Perception (S&P) Subsystem for object acquisition and tracking, and the Manipulator Control and Mechanization (MCM) Subsystem for dual-arm control. S&P acquires and tracks the position, orientation, velocity, and angular velocity of a slowly spinning satellite, and sends tracking data to the MCM subsystem. MCM grapples the satellite and brings it to rest, controlling the arms so that no excessive forces or torques are exerted on the satellite or arms. A 350-pound satellite mockup which can spin freely on a gimbal for several minutes, closely simulating the dynamics of a real satellite is demonstrated. The satellite mockup is fitted with a panel under which may be mounted various elements such as line replacement modules and electrical connectors that will be used to demonstrate servicing tasks once the satellite is docked. The subsystems are housed in three MicroVAX II microcomputers. The hardware of the S&P Subsystem includes CCD cameras, video digitizers, frame buffers, IMFEX (a custom pipelined video processor), a time-code generator with millisecond precision, and a MicroVAX II computer. Its software is written in Pascal and is based on a locally written vision software library. The hardware of the MCM Subsystem includes PUMA 560 robot arms, Lord force/torque sensors, two MicroVAX II computers, and unimation pneumatic parallel grippers. Its software is written in C, and is based on a robot language called RCCL. The two subsystems are described and test results on the grappling of the satellite mockup with rotational rates of up to 2 rpm are provided.

Investigating the origins of the Irregular satellites using Cladistics

NASA Astrophysics Data System (ADS)

Holt, Timothy; Horner, Jonti; Tylor, Christopher; Nesvorny, David; Brown, Adrian; Carter, Brad

2017-10-01

The irregular satellites of Jupiter and Saturn are thought to be objects captured during a period of instability in the early solar system. However, the precise origins of these small bodies remain elusive. We use cladistics, a technique traditionally used by biologists, to help constrain the origins of these bodies. Our research contributes to a growing body of work that uses cladistics in astronomy, collectively called astrocladistics. We present one of the first instances of cladistics being used in a planetary science context. The analysis uses physical and compositional characteristics of three prograde Jovian irregular satellites (Themisto, Leda & Himalia), five retrograde Jovian irregular satellites (Ananke, Carme, Pasiphae, Sinope & Callirrhoe), along with Phoebe, a retrograde irregular satellite of Saturn, and several other regular Jovian and Saturnian satellites. Each of these members are representatives of their respective taxonomic groups. The irregular satellites are compared with other well-studied solar system bodies, including satellites, terrestrial planets, main belt asteroids, comets, and minor planets. We find that the Jovian irregular satellites cluster with asteroids and Ceres. The Saturnian satellites studied here are found to form an association with the comets, adding to the narrative of exchange between the outer solar system and Saturnian orbital space. Both of these results demonstrate the utility of cladistics as an analysis tool for the planetary sciences.

Estimating the Earth's geometry, rotation and gravity field using a multi-satellite SLR solution

NASA Astrophysics Data System (ADS)

Stefka, V.; Blossfeld, M.; Mueller, H.; Gerstl, M.; Panafidina, N.

2012-12-01

Satellite Laser Ranging (SLR) is the unique technique to determine station coordinates, Earth Orientation Parameter (EOP) and Stokes coefficients of the Earth's gravity field in one common adjustment. These parameters form the so called "three pillars" (Plag & Pearlman, 2009) of the Global Geodetic Observing System (GGOS). In its function as official analysis center of the International Laser Ranging Service (ILRS), DGFI is developing and maintaining software to process SLR observations called "DGFI Orbit and Geodetic parameter estimation Software" (DOGS). The software is used to analyze SLR observations and to compute multi-satellite solutions. To take benefit of different orbit performances (e.g. inclination and altitude), a solution using ten different spherical satellites (ETALON1/2, LAGEOS1/2, STELLA, STARLETTE, AJISAI, LARETS, LARES, BLITS) covering the period of 12 years of observations is computed. The satellites are relatively weighted using a variance component estimation (VCE). The obtained weights are analyzed w.r.t. the potential of the satellite to monitor changes in the Earths geometry, rotation and gravity field. The estimated parameters (station coordinates and EOP) are validated w.r.t. official time series of the IERS. The Stokes coefficients are compared to recent gravity field solutions.

Evidence that acidic fibroblast growth factor promotes maturation of rat satellite-cell-derived myotubes in vitro.

PubMed

Düsterhöft, S; Pette, D

1999-11-01

Satellite cells isolated from fast tibialis anterior (TA) and slow soleus (SOL) rat muscles were cultivated on matrigel, and treated with acidic fibroblast growth factor (aFGF). The following observations were made: 1) aFGF-treated cultures exhibited enhanced proliferation as mirrored by a twofold increase in DNA content. 2) Compared to the untreated cultures, myotubes in the aFGF cultures were larger; 3) Using reverse transcriptase polymerase chain reaction (RT-PCR) and northern blot analyses, we observed enhanced expression of all adult myosin heavy chain (MHC) isoforms, as well as of myogenin. These findings indicate that, under the culture conditions used, aFGF has a stimulatory effect on proliferation but also on maturation and differentiation of satellite cells. Furthermore, transcript levels of FGF receptor 1 (FGFR1) and 4 (FGFR4) isoforms, as well as of aFGF and bFGF were assessed by RT-PCR. aFGF-treated myotubes displayed increased expression of aFGF and bFGF, suggesting a paracrine effect of exogenous aFGF. In this regard, SOL-derived cultures responded more strongly than TA-derived cultures. The effects of aFGF treatment on the two receptors consisted of a decrease in FGFR1 and an increase in FGFR4 mRNA levels in 5-day-old cultures. In 8-day-old TA cultures, effects of FGF were similar to those in 5-day-old cultures. 8-day FGF-treated SOL cultures treated with FGF for 8 days exhibited higher FGFR1 and FGFR4 mRNA levels than the respective untreated cultures. Compared to 5 day-treated cultures, FGFR1 increased and FGFR4 decreased. This led to a shift in the ratio of FGFR1 to FGFR4 in the FGF-treated cultures which may explain the ability of satellite cells to differentiate under the influence of aFGF.

Solar photovoltaic research and development program of the Air Force Aero Propulsion Laboratory. [silicon solar cell applicable to satellite power systems

NASA Technical Reports Server (NTRS)

Wise, J.

1979-01-01

Progress is reported in the following areas: laser weapon effects, solar silicon solar cell concepts, and high voltage hardened, high power system technology. Emphasis is placed on solar cells with increased energy conversion efficiency and radiation resistance characteristics for application to satellite power systems.

Whistler-like Signals Detected Simultaneously by Swarm Satellites

NASA Astrophysics Data System (ADS)

Hulot, G.; Coisson, P.; Deram, P.; Leger, J. M.; Jager, T.; Brocco, L.

2016-12-01

The three Swarm satellites embark an absolute scalar magnetometer (ASM) that generates nominally 1 Hz filtered data of the intensity of the magnetic field. Its internal frequency of operation however allows to record data at higher sampling rate, generating so-called "burst-mode" data at 250 Hz. During the commissioning phase of the Swarm mission, between December 2013 and February 2014 several burst-mode sessions have been recorded. At the beginning the satellites were still on a pearls-on-string orbital configuration and later each satellite was successively moved to its final orbit. During the last two burst sessions satellites B and C were still following each other, while satellite A was already orbiting at a lower altitude, on the opposite side of the Earth. We present an analysis of the burst data recorded on 22 and 23 February 2014, when several short signals were detected, usually lasting less than 1 second, presenting a descending tone in the frequency band accessible using burst data (1-125 Hz), similar to the whistlers signals in VLF band. These signals were detected nearly simultaneously by satellites B and C, at about 300 km distance, corresponding to a horizontal speed of about 1500 km/s. We present an analysis of these events and of their coincidence with lighting activity on the ground in the area of visibility of these satellites, as provided by the WWLN.

The incidence of satellite cysts in keratocystic odontogenic tumors.

PubMed

Pavelić, Boiidar; Katunarić, Marina; Segović, Sanja; Karadole, Maja Cimas; Katanec, Davor; Saban, Aida; Puhar, Ivan

2014-03-01

Renaming of the Odontogenic Keratocyst as the Keratocystic Odontogenic Tumor by the World Health Organization (WHO) is based on the aggressive nature of this lesion. Satellite cysts founded in the walls of the original cysts may give rise to a new lesion formation. The aim of this retrospecitve study was to identify the existence of specific features according incidence of satellite cysts and the pallisading of the basal layer of the epithelium and to establish their mutual correlation. The histopathologic data of Keratocystic Odontogenic Tumor on the basis of new WHO's classification (2005) were analized. Prominent palisade basal cell layer was found in 415 (94.75%) and partially absent palisade basal cell layer in 23 (5.25%) cases. Satellite cysts were presented in prominent palisade basal cell layer in 85 specimens (20.5%) and in cases with partial absent of the palisade basal layer in 3 spicemens (13%). The higher the frequency of pallisading was the higher the frequency of satellite cysts was (p > 0.05).

Satellite Applications for K-12 Geoscience Education

NASA Astrophysics Data System (ADS)

Mooney, M.; Ackerman, S.; Lettvin, E.; Emerson, N.; Whittaker, T. M.

2007-12-01

This presentation will highlight interactive on-line curriculum developed at the Cooperative Institute for Meteorological Satellite Studies (CIMSS) at the University of Wisconsin in Madison. CIMSS has been on the forefront of educational software design for over two decades, routinely integrating on-line activities into courses on satellite remote sensing. In 2006, CIMSS began collaborating with education experts and researchers from the University of Washington to create an NSF-funded distance learning course for science teachers called Satellite Applications for Geoscience Education. This course includes numerous web-based learning activities, including a distance education tool called VISITview which allows instructors to connect with multiple students simultaneously to conduct a lesson. Developed at CIMSS to facilitate training of National Weather Service forecasters economically and remotely, VISITview is especially effective for groups of people discussing and analyzing maps or images interactively from many locations. Along with an on-line chat function, VISITview participants can use a speaker phone or a networked voice-enabled application to create a learning environment similar to a traditional classroom. VISITview will be used in two capacities: first, instructors will convey topics of current relevance in geoscience disciplines via VISITview. Second, the content experts will participate in "virtual visits" to the classrooms of the educators who take the course for full credit. This will enable scientists to interact with both teachers and students to answer questions and discuss exciting or inspiring examples that link satellite data to their areas of research. As long as a school has Internet access, an LCD projector and a speakerphone, VISITview sessions can be shared with an entire classroom. The geoscientists who developed material for the course and conducting VISITview lectures include a geologist from the University of Wisconsin-Richland, an

Space satellite power system. [conversion of solar energy by photovoltaic solar cell arrays

NASA Technical Reports Server (NTRS)

Glaser, P. E.

1974-01-01

The concept of a satellite solar power station was studied. It is shown that it offers the potential to meet a significant portion of future energy needs, is pollution free, and is sparing of irreplaceable earth resources. Solar energy is converted by photovoltaic solar cell arrays to dc energy which in turn is converted into microwave energy in a large active phased array. The microwave energy is beamed to earth with little attenuation and is converted back to dc energy on the earth. Economic factors are considered.

ISS Expedition 18 Synchronized Position Hold,Engage,Reorient,Experimental Satellites (SPHERES)

NASA Image and Video Library

2008-10-26

ISS018-E-005214 (26 Oct. 2008) --- This close-up view shows three bowling-ball-sized free-flying satellites called Synchronized Position Hold, Engage, Reorient, Experimental Satellites (SPHERES) in the Destiny laboratory of the International Space Station. SPHERES were designed to test control algorithms for spacecraft by performing autonomous rendezvous and docking maneuvers inside the station. The results are important for multi-body control and in designing constellation and array spacecraft configurations.

Impact of static magnetic fields on human myoblast cell cultures.

PubMed

Stern-Straeter, Jens; Bonaterra, Gabriel Alejandro; Kassner, Stefan S; Faber, Anne; Sauter, Alexander; Schulz, Johannes D; Hörmann, Karl; Kinscherf, Ralf; Goessler, Ulrich Reinhart

2011-12-01

Treatment of skeletal muscle loss due to trauma or tumor ablation therapy still lacks a suitable clinical approach. Creation of functional muscle tissue in vitro using the differentiation potential of human satellite cells (myoblasts) is a promising new research field called tissue engineering. Strong differentiation stimuli, which can induce formation of myofibers after cell expansion, have to be identified and evaluated in order to create sufficient amounts of neo-tissue. The objective of this study was to determine the influence of static magnetic fields (SMF) on human satellite cell cultures as one of the preferred stem cell sources in skeletal muscle tissue engineering. Experiments were performed using human satellite cells with and without SMF stimulation after incubation with a culture medium containing low [differentiation medium (DM)] or high [growth medium (GM)] concentrations of growth factors. Proliferation analysis using the alamarBlue assay revealed no significant influence of SMF on cell division. Real-time RT-PCR of the following marker genes was investigated: myogenic factor 5 (MYF5), myogenic differentiation antigen 1 (MYOD1), myogenin (MYOG), skeletal muscle α1 actin (ACTA1), and embryonic (MYH3), perinatal (MYH8) and adult (MYH1) skeletal muscle myosin heavy chain. We detected an influence on marker gene expression by SMF in terms of a down-regulation of the marker genes in cell cultures treated with SMF and DM, but not in cell cultures treated with SMF and GM. Immunocytochemical investigations using antibodies directed against the differentiation markers confirmed the gene expression results and showed an enhancement of maturation after stimulation with GM and SMF. Additional calculation of the fusion index also revealed an increase in myotube formation in cell cultures treated with SMF and GM. Our findings show that the effect of SMF on the process of differentiation depends on the growth factor concentration in the culture medium in human

Effect of ECM2 expression on bovine skeletal muscle-derived satellite cell differentiation.

PubMed

Liu, Chang; Tong, Huili; Li, Shufeng; Yan, Yunqin

2018-05-01

Extracellular matrix components have important regulatory functions during cell proliferation and differentiation. In recent study, extracellular matrix were shown to have a strong effect on skeletal muscle differentiation. Here, we aimed to elucidate the effects of extracellular matrix protein 2 (ECM2), an extracellular matrix component, on the differentiation of bovine skeletal muscle-derived satellite cells (MDSCs). Western blot and immunofluorescence analyses were used to elucidate the ECM2 expression pattern in bovine MDSCs during differentiation in vitro. CRISPR/Cas9 technology was used to activate or inhibit ECM2 expression to study its effects on the in vitro differentiation of bovine MDSCs. ECM2 expression was shown to increase gradually during bovine MDSC differentiation, and the levels of this protein were higher in more highly differentiated myotubes. ECM2 activation promoted MDSC differentiation, whereas its suppression inhibited the differentiation of these cells. Here, for the first time, we demonstrated the importance of ECM2 expression during bovine MDSC differentiation; these results could lead to treatments that help to increase beef cattle muscularity. © 2018 International Federation for Cell Biology.

Satellite Data Inform Forecasts of Crop Growth

NASA Technical Reports Server (NTRS)

2015-01-01

During a Stennis Space Center-led program called Ag 20/20, an engineering contractor developed models for using NASA satellite data to predict crop yield. The model was eventually sold to Genscape Inc., based in Louisville, Kentucky, which has commercialized it as LandViewer. Sold under a subscription model, LandViewer software provides predictions of corn production to ethanol plants and grain traders.

Satellite Delivery of Aviation Weather Data

NASA Technical Reports Server (NTRS)

Kerczewski, Robert J.; Haendel, Richard

2001-01-01

With aviation traffic continuing to increase worldwide, reducing the aviation accident rate and aviation schedule delays is of critical importance. In the United States, the National Aeronautics and Space Administration (NASA) has established the Aviation Safety Program and the Aviation System Capacity Program to develop and test new technologies to increase aviation safety and system capacity. Weather is a significant contributor to aviation accidents and schedule delays. The timely dissemination of weather information to decision makers in the aviation system, particularly to pilots, is essential in reducing system delays and weather related aviation accidents. The NASA Glenn Research Center is investigating improved methods of weather information dissemination through satellite broadcasting directly to aircraft. This paper describes an on-going cooperative research program with NASA, Rockwell Collins, WorldSpace, Jeppesen and American Airlines to evaluate the use of satellite digital audio radio service (SDARS) for low cost broadcast of aviation weather information, called Satellite Weather Information Service (SWIS). The description and results of the completed SWIS Phase 1 are presented, and the description of the on-going SWIS Phase 2 is given.

High Specific Energy NiH2 Batteries for GEO Satellites

NASA Technical Reports Server (NTRS)

Borthomieu, Y.; Fabre, M.

2001-01-01

This viewgraph presentation outlines the SAFT qualification status history, cell and battery modifications, overall battery characteristics, satellite programs and battery types delivered, and battery performances for selected satellite missions.

ACTS TDMA network control. [Advanced Communication Technology Satellite

NASA Technical Reports Server (NTRS)

Inukai, T.; Campanella, S. J.

1984-01-01

This paper presents basic network control concepts for the Advanced Communications Technology Satellite (ACTS) System. Two experimental systems, called the low-burst-rate and high-burst-rate systems, along with ACTS ground system features, are described. The network control issues addressed include frame structures, acquisition and synchronization procedures, coordinated station burst-time plan and satellite-time plan changes, on-board clock control based on ground drift measurements, rain fade control by means of adaptive forward-error-correction (FEC) coding and transmit power augmentation, and reassignment of channel capacities on demand. The NASA ground system, which includes a primary station, diversity station, and master control station, is also described.

LANDSAT language at our reach. First Swedish satellite. Civilization detectors

NASA Technical Reports Server (NTRS)

Wayne, D. L.; Bravo, V.

1981-01-01

Information on the use of LANDSAT data by Argentina is presented. Details on a Swedish satellite to be completed in 1984 and to be called VIKING are reported. Attempts to contact other civilizations in space by the use of radiotelescopes are discussed.

Satellite Power Systems (SPS) concept definition study (Exhibit D). Volume 4: Operations analyses

NASA Technical Reports Server (NTRS)

Hanley, G. M.

1981-01-01

Using a coplanar, end-mounted antenna as a baseline, various configuration trades were performed to select a preferred solid state concept. The increase in efficiency that could be realized by use of multi bandgap solar cells, either with klystron or solid state antenna was evaluated. Satellite configurations were developed to exploit the sandwich antenna concept wherein solar cells are located on one side of the antenna panel and solid state dc/RF converters on the other side. These concepts entailed various primary and secondary reflector arrangements for directing solar energy to the solar cell side of the antenna with higher concentration ratios than used on the coplanar configurations. Operations analysis included development of a satellite construction scenario, a concept for the SCB, a top-level satellite construction operation, construction operation, construction timelines and crew sizes, mass flows to orbit, and a satellite maintenance scenario. The list of materials required for satellite construction was updated to identify significant differences relevant to the solid state satellite concept. Means of decommissioning satellites at the end of their design life were studied.

Data Collection Satellite Application in Precision Agriculture

NASA Astrophysics Data System (ADS)

Durào, O.

2002-01-01

Agricultural Instrumentation Research Center, Brazilian Agricultural Research Corporation; Space Programs Brazil launched in 1993 its first satellite partially built and entirely designed, integrated, tested and operated in the country. It was the SCD-1 satellite, a small (115 kg. and an octagonal prism with 80 cm. height and an external diameter of 100 cm.) with a payload transponder that receives data from ground platforms spread all over the country (including its sea shore). These data are then retransmitted to a receiving station at every satellite pass. Data collected and received are processed at Data Collection Mission Center for distribution via internet at most 30 min after the satellite pass. The ground platforms are called PCD's and differ in the parameters measured according to its purpose and location. Thus, they are able to measure temperature, rain level, wind direction, solar radiation, carbon monoxide as well as many others, beyond its own location. SCD- 1 had a nominal designed life of one year, but is still functioning. It is a LEO satellite with inclination of 25°. In 1998, the country launched SCD-2, with the same purpose, but in phase with SCD-1 . Other differences were a higher index of Brazilian made components and an active attitude control subsystem for the spin rate provided by the magnetic torque coils (these in accordance with a development strategy previously planned). In 1999 the country launched in cooperation with China a remote sensing satellite (mass of 1.4 ton.) called CBERS-1. This satellite is sun synchronous (98° inclination) and also carries a transponder for data collection/transmission as a secondary payload. Thus, the country has now three satellites with data collection/transmission capabilities, two in low inclination phased orbits and one in polar orbit, providing a nice coverage both geographical and temporal not only to its territory but also to other regions of the world.. At first there were not too many PCD

Spiny Nanorod and Upconversion Nanoparticle Satellite Assemblies for Ultrasensitive Detection of Messenger RNA in Living Cells.

PubMed

Gao, Rui; Hao, Changlong; Xu, Liguang; Xu, Chuanlai; Kuang, Hua

2018-04-17

Quantitation and in situ monitoring of target mRNA (mRNA) in living cells remains a significant challenge for the chemical and biomedical communities. To quantitatively detect mRNA expression levels in living cells, we have developed DNA-driven gold nanorod coated platinum-upconversion nanoparticle satellite assemblies (termed Au NR@Pt-UCNP satellites) for intracellular thymidine kinase 1 (TK1) mRNA analysis. The nanostructures were capable of recognizing target mRNA in a sequence-specific manner as luminescence of UCNPs was effectively quenched by Au NR@Pt within the assemblies. Following recognition, UCNPs detached from Au NR@Pt, resulting in luminescence restoration to achieve effective in situ imaging and quantifiable detection of target mRNA. The upconversional luminescence intensity of confocal images showed a good linear relationship with intracellular TK1 mRNA ranging from 1.17 to 65.21 fmol/10 μg RNA and a limit of detection (LOD) of 0.67 fmol/10 μg RNA. We believe that our present assay can be broadly applied for detection of endogenous biomolecules at the cellular and tissue levels and restoration of tissue homeostasis in vivo.

The quasi-parallel lives of satellite cells and atrophying muscle

PubMed Central

Biressi, Stefano; Gopinath, Suchitra D.

2015-01-01

Skeletal muscle atrophy or wasting accompanies various chronic illnesses and the aging process, thereby reducing muscle function. One of the most important components contributing to effective muscle repair in postnatal organisms, the satellite cells (SCs), have recently become the focus of several studies examining factors participating in the atrophic process. We critically examine here the experimental evidence linking SC function with muscle loss in connection with various diseases as well as aging, and in the subsequent recovery process. Several recent reports have investigated the changes in SCs in terms of their differentiation and proliferative capacity in response to various atrophic stimuli. In this regard, we review the molecular changes within SCs that contribute to their dysfunctional status in atrophy, with the intention of shedding light on novel potential pharmacological targets to counteract the loss of muscle mass. PMID:26257645

Embryonic-only arsenic exposure alters skeletal muscle satellite cell function in killifish (Fundulus heteroclitus).

PubMed

Szymkowicz, Dana B; Schwendinger, Katey L; Tatnall, Caroline M; Swetenburg, John R; Bain, Lisa J

2018-05-01

Arsenic is a contaminant found worldwide in drinking water and food. Epidemiological studies have correlated arsenic exposure with reduced weight gain and improper muscular development, while in vitro studies show that arsenic exposure impairs myogenic differentiation. The purpose of this study was to use Fundulus heteroclitus or killifish as a model organism to determine if embryonic-only arsenic exposure permanently reduces the number or function of muscle satellite cells. Killifish embryos were exposed to 0, 50, 200, or 800 ppb arsenite (As III ) until hatching, and then juvenile fish were raised in clean water. At 28, 40, and 52 weeks after hatching, skeletal muscle injuries were induced by injecting cardiotoxin into the trunk of the fish just posterior to the dorsal fin. Muscle sections were collected at 0, 3 and 10 days post-injury. Collagen levels were used to assess muscle tissue damage and recovery, while levels of proliferating cell nuclear antigen (PCNA) and myogenin were quantified to compare proliferating cells and newly formed myoblasts. At 28 weeks of age, baseline collagen levels were 105% and 112% greater in 200 and 800 ppb groups, respectively, and at 52 weeks of age, were 58% higher than controls in the 200 ppb fish. After cardiotoxin injury, collagen levels tend to increase to a greater extent and take longer to resolve in the arsenic exposed fish. The number of baseline PCNA(+) cells were 48-216% greater in 800 ppb exposed fish compared to controls, depending on the week examined. However, following cardiotoxin injury, PCNA is reduced at 28 weeks in 200 and 800 ppb fish at day 3 during the recovery period. By 52 weeks, there are significant reductions in PCNA in all exposure groups at day 3 of the recovery period. Based on these results, embryonic arsenic exposure increases baseline collagen levels and PCNA(+) cells in skeletal muscle. However, when these fish are challenged with a muscle injury, the proliferation and

An advanced generation land mobile satellite system and its critical technologies

NASA Technical Reports Server (NTRS)

Naderi, F.

1982-01-01

A conceptual design for a Land Mobile Satellite System (LMSS) for the 1990s is presented. LMSS involves small tranceivers accessing satellites directly, with ground reception through small car-top antennas. The satellite would have a large antenna and blanket coverage areas in the UHF. The call may originate from a home, be carried by wire to a gateway, transmitted to satellite on the S-band, converted to UHF on the satellite, and transmitted to the vehicle. The system design is constrained by the number of users in an area during the busiest hours, Shuttle storage, controllability factors, and the total area served. A 55-m antenna has been selected, with 87 spot beams and two 10 MHz UHF bands in the 806-890 MHz band. A 17 dB interbeam isolation level is required, implying that sufficient sub-bands can be generated to assure 8265 total channels. The mobile satellite (MSAT) would have an 83 m mast lower segment, a 34 m upper segment, and a second, 10 m antenna made of a deployable mesh. Various antenna function modes are considered.

A Battery Certification Testbed for Small Satellite Missions

NASA Technical Reports Server (NTRS)

Cameron, Zachary; Kulkarni, Chetan S.; Luna, Ali Guarneros; Goebel, Kai; Poll, Scott

2015-01-01

A battery pack consisting of standard cylindrical 18650 lithium-ion cells has been chosen for small satellite missions based on previous flight heritage and compliance with NASA battery safety requirements. However, for batteries that transit through the International Space Station (ISS), additional certification tests are required for individual cells as well as the battery packs. In this manuscript, we discuss the development of generalized testbeds for testing and certifying different types of batteries critical to small satellite missions. Test procedures developed and executed for this certification effort include: a detailed physical inspection before and after experiments; electrical cycling characterization at the cell and pack levels; battery-pack overcharge, over-discharge, external short testing; battery-pack vacuum leak and vibration testing. The overall goals of these certification procedures are to conform to requirements set forth by the agency and identify unique safety hazards. The testbeds, procedures, and experimental results are discussed for batteries chosen for small satellite missions to be launched from the ISS.

Current developments in electrochemical storage systems for satellites

NASA Technical Reports Server (NTRS)

Gutmann, G.

1986-01-01

The need for batteries with greater power capacity and service life for power satellites is examined. The Ni/Cd and Ni/H batteries now being used must be upgraded to meet advanced space requirements. Improvements in power capacity, service life, and cycle count for various satellites in LEO and GEO orbits are discussed. The Ni/Cd and Ni/H cell reactions are explained, and the solubility and volume changes for various charged and uncharged masses are described. A chart of the energy content and cycle count for various cell systems is presented, and the factors which cause aging and failure in the Ni/Cd and Ni/H cell systems are discussed. The advantages of the Ni/H battery are given and the need for more developed electrochemical storage systems because of an increase in the mass of satellites is explained. The requirements for space batteries and the work currently done by NASA and West Germany on advanced batteries are discussed.

Economic importance of transportation services : highlights of the Transportation Satellite Accounts

DOT National Transportation Integrated Search

1998-04-01

A new accounting tool, called the Transportation Satellite Accounts (TSA), now provides a way to measure both in-house and for-hire transportation services. The TSA, developed jointly by the Bureau of Transportation Statistics (BTS) of the Department...

Weakly stationary noise filtering of satellite-acquired imagery

NASA Technical Reports Server (NTRS)

Palgen, J. J. O.; Tamches, I.; Deutsch, E. S.

1971-01-01

A type of weakly stationary noise called herringbone noise was observed in satellite imagery. The characteristics of this noise are described; a model for its simulation was developed. The model is used to degrade pictorial data for comparison with similar noise degraded Nimbus data. Two filtering methods are defined and evaluated. A user's application demonstration is discussed.

The Globalstar mobile satellite system for worldwide personal communications

NASA Astrophysics Data System (ADS)

Wiedeman, Robert A.; Viterbi, Andrew J.

Loral Aerospace Corporation along with Qualcomm Inc. have developed a satellite system which offers global mobile voice and data services to and from handheld and mobile user terminals with omni-directional antennas. By combining the use of low-earth orbit (LEO) satellites with existing terrestrial communications systems and innovative, highly efficient spread spectrum techniques, the Globalstar system provides users with low-cost, reliable communications throughout the world. The Globalstar space segment consists of a constellation of 48 LEO satellites in circular orbits with 750 NM (1389 km) altitude. Each satellite communicates with the mobile users via the satellite-user links and with gateway stations. The gateway stations handle the interface between the Globalstar network and the OSTN/PLMN systems. Globalstar transceivers are similar to currently proposed digital cellular telephones in size and have a serial number that will allow the end user to make and receive calls from or to that device anywhere in the world. The Globalstar system is designed to operate as a complement to existing local, long-distance, public, private and specialized telecommunications networks. Service is primarily designed to serve the rural and thin route communications needs of consumers, government users, and private networks.

The Globalstar mobile satellite system for worldwide personal communications

NASA Technical Reports Server (NTRS)

Wiedeman, Robert A.; Viterbi, Andrew J.

1993-01-01

Loral Aerospace Corporation along with Qualcomm Inc. have developed a satellite system which offers global mobile voice and data services to and from handheld and mobile user terminals with omni-directional antennas. By combining the use of low-earth orbit (LEO) satellites with existing terrestrial communications systems and innovative, highly efficient spread spectrum techniques, the Globalstar system provides users with low-cost, reliable communications throughout the world. The Globalstar space segment consists of a constellation of 48 LEO satellites in circular orbits with 750 NM (1389 km) altitude. Each satellite communicates with the mobile users via the satellite-user links and with gateway stations. The gateway stations handle the interface between the Globalstar network and the OSTN/PLMN systems. Globalstar transceivers are similar to currently proposed digital cellular telephones in size and have a serial number that will allow the end user to make and receive calls from or to that device anywhere in the world. The Globalstar system is designed to operate as a complement to existing local, long-distance, public, private and specialized telecommunications networks. Service is primarily designed to serve the rural and thin route communications needs of consumers, government users, and private networks.

Macrophage deficiency in osteopetrotic (op/op) mice inhibits activation of satellite cells and prevents hypertrophy in single soleus fibers.

PubMed

Ohira, T; Wang, X D; Ito, T; Kawano, F; Goto, K; Izawa, T; Ohno, H; Kizaki, T; Ohira, Y

2015-05-15

Effects of macrophage on the responses of soleus fiber size to hind limb unloading and reloading were studied in osteopetrotic homozygous (op/op) mice with inactivated mutation of macrophage colony-stimulating factor (M-CSF) gene and in wild-type (+/+) and heterozygous (+/op) mice. The basal levels of mitotically active and quiescent satellite cell (-46 and -39% vs. +/+, and -40 and -30% vs. +/op) and myonuclear number (-29% vs. +/+ and -28% vs. +/op) in fibers of op/op mice were significantly less than controls. Fiber length and sarcomere number in op/op were also less than +/+ (-22%) and +/op (-21%) mice. Similar trend was noted in fiber cross-sectional area (CSA, -15% vs. +/+, P = 0.06, and -14% vs. +/op, P = 0.07). The sizes of myonuclear domain, cytoplasmic volume per myonucleus, were identical in all types of mice. The CSA, length, and the whole number of sarcomeres, myonuclei, and mitotically active and quiescent satellite cells, as well as myonuclear domain, in single muscle fibers were decreased after 10 days of unloading in all types of mice, although all of these parameters in +/+ and +/op mice were increased toward the control values after 10 days of reloading. However, none of these levels in op/op mice were recovered. Data suggest that M-CSF and/or macrophages are important to activate satellite cells, which cause increase of myonuclear number during fiber hypertrophy. However, it is unclear why their responses to general growth and reloading after unloading are different. Copyright © 2015 the American Physiological Society.

Follistatin induces muscle hypertrophy through satellite cell proliferation and inhibition of both myostatin and activin.

PubMed

Gilson, Hélène; Schakman, Olivier; Kalista, Stéphanie; Lause, Pascale; Tsuchida, Kunihiro; Thissen, Jean-Paul

2009-07-01

Follistatin (FS) inhibits several members of the TGF-beta superfamily, including myostatin (Mstn), a negative regulator of muscle growth. Mstn inhibition by FS represents a potential therapeutic approach of muscle atrophy. The aim of our study was to investigate the mechanisms of the FS-induced muscle hypertrophy. To test the role of satellite cells in the FS effect, we used irradiation to destroy their proliferative capacity. FS overexpression increased the muscle weight by about 37% in control animals, but the increase reached only 20% in irradiated muscle, supporting the role of cell proliferation in the FS-induced hypertrophy. Surprisingly, the muscle hypertrophy caused by FS reached the same magnitude in Mstn-KO as in WT mice, suggesting that Mstn might not be the only ligand of FS involved in the regulation of muscle mass. To assess the role of activin (Act), another FS ligand, in the FS-induced hypertrophy, we electroporated FSI-I, a FS mutant that does not bind Act with high affinity. Whereas FS electroporation increased muscle weight by 32%, the muscle weight gain induced by FSI-I reached only 14%. Furthermore, in Mstn-KO mice, FSI-I overexpression failed to induce hypertrophy, in contrast to FS. Therefore, these results suggest that Act inhibition may contribute to FS-induced hypertrophy. Finally, the role of Act as a regulator of muscle mass was supported by the observation that ActA overexpression induced muscle weight loss (-15%). In conclusion, our results show that satellite cell proliferation and both Mstn and Act inhibition are involved in the FS-induced muscle hypertrophy.

Possible misinterpretation of lunar cratering record in Voyager team analyses of outer planet satellites

NASA Technical Reports Server (NTRS)

Hartmann, William K.

1991-01-01

While interpreting outer planetary satellites, the Voyager imaging team repeatedly referred to a lunar frontside highland calibration curve. It was assumed that it is unmodified and not in steady state equilibrium, but rather records all impacts that have occurred. It was also assumed that it records the size distribution of an early population of impactors, called Population I, evidence for which was found on various satellites. New evidence is reported that the Voyager team interpretation of this population is wrong, a conclusion that seriously affects the cratering histories reported for outer planet satellites.

Lightweight Solar Power for Small Satellites

NASA Technical Reports Server (NTRS)

Nabors, Sammy A.

2015-01-01

The innovation targets small satellites or CubeSats for which conventional deployable arrays are not feasible due to their size, weight and complexity. This novel solar cell array includes a thin and flexible photovoltaic cell applied to an inflatable structure to create a high surface area array for collecting solar energy in a lightweight, simple and deployable structure. The inflatable array, with its high functional surface area, eliminates the need and the mechanisms required to point the system toward the sun. The power density achievable in these small arrays is similar to that of conventional high-power deployable/pointable arrays used on large satellites or space vehicles. Although inflatable solar arrays have been previously considered by others, the arrays involved the use of traditional rigid solar cells. Researchers are currently working with thin film photovoltaics from various suppliers so that the NASA innovation is not limited to any particular solar cell technology. NASA has built prototypes and tested functionality before and after inflation. As shown in the current-voltage currents below, deployment does not damage the cell performance.

Calling patterns in human communication dynamics

PubMed Central

Jiang, Zhi-Qiang; Xie, Wen-Jie; Li, Ming-Xia; Podobnik, Boris; Zhou, Wei-Xing; Stanley, H. Eugene

2013-01-01

Modern technologies not only provide a variety of communication modes (e.g., texting, cell phone conversation, and online instant messaging), but also detailed electronic traces of these communications between individuals. These electronic traces indicate that the interactions occur in temporal bursts. Here, we study intercall duration of communications of the 100,000 most active cell phone users of a Chinese mobile phone operator. We confirm that the intercall durations follow a power-law distribution with an exponential cutoff at the population level but find differences when focusing on individual users. We apply statistical tests at the individual level and find that the intercall durations follow a power-law distribution for only 3,460 individuals (3.46%). The intercall durations for the majority (73.34%) follow a Weibull distribution. We quantify individual users using three measures: out-degree, percentage of outgoing calls, and communication diversity. We find that the cell phone users with a power-law duration distribution fall into three anomalous clusters: robot-based callers, telecom fraud, and telephone sales. This information is of interest to both academics and practitioners, mobile telecom operators in particular. In contrast, the individual users with a Weibull duration distribution form the fourth cluster of ordinary cell phone users. We also discover more information about the calling patterns of these four clusters (e.g., the probability that a user will call the cr-th most contact and the probability distribution of burst sizes). Our findings may enable a more detailed analysis of the huge body of data contained in the logs of massive users. PMID:23319645

Calling patterns in human communication dynamics.

PubMed

Jiang, Zhi-Qiang; Xie, Wen-Jie; Li, Ming-Xia; Podobnik, Boris; Zhou, Wei-Xing; Stanley, H Eugene

2013-01-29

Modern technologies not only provide a variety of communication modes (e.g., texting, cell phone conversation, and online instant messaging), but also detailed electronic traces of these communications between individuals. These electronic traces indicate that the interactions occur in temporal bursts. Here, we study intercall duration of communications of the 100,000 most active cell phone users of a Chinese mobile phone operator. We confirm that the intercall durations follow a power-law distribution with an exponential cutoff at the population level but find differences when focusing on individual users. We apply statistical tests at the individual level and find that the intercall durations follow a power-law distribution for only 3,460 individuals (3.46%). The intercall durations for the majority (73.34%) follow a Weibull distribution. We quantify individual users using three measures: out-degree, percentage of outgoing calls, and communication diversity. We find that the cell phone users with a power-law duration distribution fall into three anomalous clusters: robot-based callers, telecom fraud, and telephone sales. This information is of interest to both academics and practitioners, mobile telecom operators in particular. In contrast, the individual users with a Weibull duration distribution form the fourth cluster of ordinary cell phone users. We also discover more information about the calling patterns of these four clusters (e.g., the probability that a user will call the c(r)-th most contact and the probability distribution of burst sizes). Our findings may enable a more detailed analysis of the huge body of data contained in the logs of massive users.

β-Hydroxy-β-methylbutyrate (HMB) enhances the proliferation of satellite cells in fast muscles of aged rats during recovery from disuse atrophy.

PubMed

Alway, Stephen E; Pereira, Suzette L; Edens, Neile K; Hao, Yanlei; Bennett, Brian T

2013-09-01

Loss of myonuclei by apoptosis is thought to contribute to sarcopenia. We have previously shown, that the leucine metabolite, β-hydroxy-β-methylbutyrate (HMB) suppresses apoptotic signaling and the apoptotic index (the ratio of apoptotic positive to apoptotic negative myonuclei) during muscle disuse and during reloading periods after disuse in aged rats. However, it was not clear if the apoptotic signaling indexes were due only to preservation of myonuclei or if perhaps the total myogenic pool increased as a result of HMB-mediated satellite cell proliferation as this would have also reduced the apoptotic index. In this study, we tested the hypothesis that HMB would augment myogenic cells (satellite cells) proliferation during muscle recovery (growth) after a period of disuse in senescent animals. The hindlimb muscles of 34 month old Fisher 344 × Brown Norway rats were unloaded for 14 days by hindlimb suspension (HLS), and then reloaded for 14 days. The rats received either Ca-HMB (340 mg/kg body weight; n = 16), or the vehicle (n = 10) by gavage throughout the experimental period. HMB prevented the functional decline in maximal plantar flexion isometric force production during the reloading period, but not during HLS. HMB-treatment enhanced the proliferation of muscle stem cells as shown by a greater percentage of satellite cells that had proliferated (more BrdU positive, Pax-7 positive, and more Pax7/Ki67 positive nuclei) and as a result, more differentiated stem cells were present (more MyoD/myogenin positive myonuclei), relative to total myonuclei, in reloaded plantaris muscles as compared to reloaded muscles from vehicle-treated animals. Furthermore HMB increased the nuclear protein abundance of proliferation markers, inhibitor of differentiation-2 and cyclin A, as compared to vehicle treatment in reloaded muscles. Although HMB increased phosphorylated Akt during reloading, other mTOR related proteins were not altered by HMB treatment. These data show that

Laser Communication Experiments with Artemis Satellite

NASA Astrophysics Data System (ADS)

Kuzkov, Sergii; Sodnik, Zoran; Kuzkov, Volodymyr

2013-10-01

In November 2001, the European Space Agency (ESA) established the world-first inter-satellite laser communication link between the geostationary ARTEMIS satellite and the low Earth orbiting (LEO) SPOT-4 Earth observation satellite, demonstrating data rates of 50 Mbps. In 2006, the Japanese Space Agency launched the KIRARI (OICETS) LEO satellite with a compatible laser communication terminal and bidirectional laser communication links (50 Mbps and 2 Mbps) were successfully realized between KIRARI and ARTEMIS. ESA is now developing the European Data Relay Satellite (EDRS) system, which will use laser communication technology to transmit data between the Sentinel 1 and 2 satellites in LEO to two geostationary satellites (EDRS-A and EDRS-C) at data rates of 1.8 Gbps. As the data handling capabilities of state-of-the-art telecommunication satellites in GEO increase so is the demand for the feeder-link bandwidth to be transmitted from ground. This is why there is an increasing interest in developing high bandwidth ground-to-space laser communication systems working through atmosphere. In 2002, the Main Astronomical Observatory (MAO) started the development of its own laser communication system for its 0.7m AZT-2 telescope, located in Kyiv, Ukraine. The work was supported by the National Space Agency of Ukraine and by ESA. MAO developed a highly accurate computerized tracking system for AZT-2 telescope and a compact laser communication package called LACES (Laser Atmosphere and Communication Experiments with Satellites). The LACES instrument includes a camera of the pointing and tracking subsystems, a receiver module, a laser transmitter module, a tip/tilt atmospheric turbulence compensation subsystem, a bit error rate tester module and other optical and electronic components. The principal subsystems are mounted on a platform, which is located at the Cassegrain focus of the AZT-2 telescope. All systems were tested with the laser communication payload on-board ARTEMIS and

Application of satellite data to tropic-subtropic moisture coupling

NASA Technical Reports Server (NTRS)

Thompson, Aylmer H.; Mcguirk, James P.

1987-01-01

Common tropical synoptic events, called moisture bursts, have been defined in terms of their appearance in infrared satellite imagery. Their synoptic and climatological behavior over the tropical North Pacific Ocean is described using data from four cool seasons, including the 1982 to 1983 El Nino winter and the January and May of 1979.

The Tracking & Data Relay Satellite System. The New Space Network.

ERIC Educational Resources Information Center

Froehlich, Walter

This publication describes the giant-capacity space communications installation called the "Tracking and Data Relay Satellite System" (TDRSS). Chapters include: (1) "A New Communications Bridge to Orbit" (illustrating what it is and how it looks); (2) "TDRSS Goes to Work" (describing how it functions); (3) "The…

Rain volume estimation over areas using satellite and radar data

NASA Technical Reports Server (NTRS)

Doneaud, A. A.; Vonderhaar, T. H.

1985-01-01

An investigation of the feasibility of rain volume estimation using satellite data following a technique recently developed with radar data called the Arera Time Integral was undertaken. Case studies were selected on the basis of existing radar and satellite data sets which match in space and time. Four multicell clusters were analyzed. Routines for navigation remapping amd smoothing of satellite images were performed. Visible counts were normalized for solar zenith angle. A radar sector of interest was defined to delineate specific radar echo clusters for each radar time throughout the radar echo cluster lifetime. A satellite sector of interest was defined by applying small adjustments to the radar sector using a manual processing technique. The radar echo area, the IR maximum counts and the IR counts matching radar echo areas were found to evolve similarly, except for the decaying phase of the cluster where the cirrus debris keeps the IR counts high.

Electrospun Fe3O4/TiO2 hybrid nanofibers and their in vitro biocompatibility: prospective matrix for satellite cell adhesion and cultivation.

PubMed

Amna, Touseef; Hassan, M Shamshi; Van Ba, Hoa; Khil, Myung-Seob; Lee, Hak-Kyo; Hwang, I H

2013-03-01

We report the fabrication of novel Fe3O4/TiO2 hybrid nanofibers with the improved cellular response for potential tissue engineering applications. In this study, Fe3O4/TiO2 hybrid nanofibers were prepared by facile sol-gel electrospinning using titanium isopropoxide and iron(III) nitrate nonahydrate as precursors. The obtained electrospun nanofibers were vacuum dried at 80 °C and then calcined at 500 °C. The physicochemical characterization of the synthesized composite nanofibers was carried out by scanning electron microscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy and X-ray diffraction pattern. To examine the in vitro cytotoxicity, satellite cells were treated with as-prepared Fe3O4/TiO2 and the viability of cells was analyzed by Cell Counting Kit-8 assay at regular time intervals. The morphological features of unexposed satellite cells and exposed to Fe3O4/TiO2 composite were examined with a phase contrast microscope whereas the quantification of cell viability was carried out via confocal laser scanning microscopy. The morphology of the cells attached to hybrid matrix was observed by Bio-SEM. Cytotoxicity experiments indicated that the satellite cells could attach to the Fe3O4/TiO2 composite nanofibers after being cultured. We observed that Fe3O4-TiO2 composite nanofibers could support cell adhesion and growth. Results from this study therefore suggest that Fe3O4/TiO2 composite scaffold with small diameters (approximately 200 nm) can mimic the natural extracellular matrix well and provide possibilities for diverse applications in the field of tissue engineering and regenerative medicine. Copyright © 2012 Elsevier B.V. All rights reserved.

The role of radiation hard solar cells in minimizing the costs of global satellite communication systems

NASA Technical Reports Server (NTRS)

Summers, Geoffrey P.; Walters, Robert J.; Messenger, Scott R.; Burke, Edward A.

1996-01-01

An analysis embodied in a PC computer program is presented, which quantitatively demonstrates how the availability of radiation hard solar cells can help minimize the cost of a global satellite communications system. An important distinction between the currently proposed systems, such as Iridium, Odyssey and Ellipsat, is the number of satellites employed and their operating altitudes. Analysis of the major costs associated with implementing these systems shows that operation at orbital altitudes within the earth's radiation belts (10(exp 3) to 10(exp 4)km) can reduce the total cost of a system by several hundred percent, so long as radiation hard components including solar cells can be used. A detailed evaluation of the predicted performance of photovoltaic arrays using several different planar solar cell technologies is given, including commercially available Si and GaAs/Ge, and InP/Si which is currently under development. Several examples of applying the program are given, which show that the end of life (EOL) power density of different technologies can vary by a factor of ten for certain missions. Therefore, although a relatively radiation-soft technology can usually provide the required EOL power by simply increasing the size of the array, the impact upon the total system budget could be unacceptable, due to increased launch and hardware costs. In aggregate, these factors can account for more than a 10% increase in the total system cost. Since the estimated total costs of proposed global-coverage systems range from $1B to $9B, the availability of radiation-hard solar cells could make a decisive difference in the selection of a particular constellation architecture.

Cladistical Analysis of the Jovian and Saturnian Satellite Systems

NASA Astrophysics Data System (ADS)

Holt, Timothy. R.; Brown, Adrian. J.; Nesvorný, David; Horner, Jonathan; Carter, Brad

2018-06-01

Jupiter and Saturn each have complex systems of satellites and rings. These satellites can be classified into dynamical groups, implying similar formation scenarios. Recently, a larger number of additional irregular satellites have been discovered around both gas giants that have yet to be classified. The aim of this paper is to examine the relationships between the satellites and rings of the gas giants, using an analytical technique called cladistics. Cladistics is traditionally used to examine relationships between living organisms, the “tree of life.” In this work, we perform the first cladistical study of objects in a planetary science context. Our method uses the orbital, physical, and compositional characteristics of satellites to classify the objects in the Jovian and Saturnian systems. We find that the major relationships between the satellites in the two systems, such as families, as presented in previous studies, are broadly preserved. In addition, based on our analysis of the Jovian system, we identify a new retrograde irregular family, the Iocaste family, and suggest that the Phoebe family of the Saturnian system can be further divided into two subfamilies. We also propose that the Saturnian irregular families be renamed, to be consistent with the convention used in Jovian families. Using cladistics, we are also able to assign the new unclassified irregular satellites into families. Taken together, the results of this study demonstrate the potential use of the cladistical technique in the investigation of relationships between orbital bodies.

Pseudo-coherent demodulation for mobile satellite systems

NASA Technical Reports Server (NTRS)

Divsalar, Dariush; Simon, Marvin K.

1993-01-01

This paper proposes three so-called pseudo-coherent demodulation schemes for use in land mobile satellite channels. The schemes are derived based on maximum likelihood (ML) estimation and detection of an N-symbol observation of the received signal. Simulation results for all three demodulators are presented to allow comparison with the performance of differential PSK (DPSK) and ideal coherent demodulation for various system parameter sets of practical interest.

Odyssey personal communications satellite system

NASA Technical Reports Server (NTRS)

Spitzer, Christopher J.

1993-01-01

The spectacular growth of cellular telephone networks has proved the demand for personal communications. Large regions of the world are too sparsely populated to be economically served by terrestrial cellular communications. Since satellites are well suited to this application, TRW filed with the FCC on May 31, 1993 for the Odyssey construction permit. Odyssey will provide high quality wireless communication services worldwide from satellites. These services will include: voice, data, paging, and messaging. Odyssey will be an economical approach to providing communications. A constellation of 12 satellites will be orbited in three, 55 deg. inclined planes at an altitude of 10,354 km to provide continuous coverage of designated regions. Two satellites will be visible anywhere in the world at all times. This dual visibility leads to high line-of-sight elevation angles, minimizing obstructions by terrain, trees and buildings. Each satellite generates a multibeam antenna pattern that divides its coverage area into a set of contiguous cells. The communications system employs spread spectrum CDMA on both the uplinks and downlinks. This signaling method permits band sharing with other systems and applications. Signal processing is accomplished on the ground at the satellite's 'Gateway' stations. The 'bent pipe' transponders accommodates different regional standards, as well as signaling changes over time. The low power Odyssey handset will be cellular compatible. Multipath fade protection is provided in the handset.

Magnetic field experiment on the SUNSAT satellite

NASA Astrophysics Data System (ADS)

Kotzé, P. B.; Langenhoven, B.; Risbo, T.

2002-03-01

On Tuesday 23 February 1999, at 10:29 UTC, SUNSAT was launched into an 857×655 km, 96.47° polar orbit on a Boeing-Delta II rocket from Vandenberg Air Force Base in California, USA. Both SUNSAT and Ørsted were NASA-sponsored secondary payloads accompanying the USA Air Force Argos satellite. In the process it became South Africa's (and Africa's) first satellite in space. Although sponsored by several private industrial organisations, it is essentially a student project with more than 96 graduate students in the Department of Electronic and Electrical Engineering at the University of Stellenbosch providing the majority of SUNSAT's engineering development and operation since 1992. This paper reports on the magnetic field experiment on board the Sunsat satellite, consisting of two fluxgate magnetometers, called Orimag and Scimag, both built and calibrated by the Hermanus Magnetic Observatory. Orimag is mainly used for orientation control purposes on SUNSAT, while Scimag, mounted on a boom of 2.2 m is designed to perform geomagnetic field observations, employing standard navigation fluxgate technology.

Development of a demand assignment/TDMA system for international business satellite communications

NASA Astrophysics Data System (ADS)

Nohara, Mitsuo; Takeuchi, Yoshio; Takahata, Fumio; Hirata, Yasuo; Yamazaki, Yoshiharu

An experimental IBS (international business satellite) communications system based on a demand assignment and TDMA (time-division multiple-access) operation has been developed. The system utilizes a limited satellite resource efficiently and provides various kinds of ISDN services totally. A discussion is presented of the IBS network configurations suitable to international communications and describes the developed communications system from the viewpoint of the hardware and software implementation. The performance in terms of the transmission quality and call processing is also demonstrated.

Deliberate Satellite Fragmentations and their Effects on the Long-Term Space Environment

NASA Technical Reports Server (NTRS)

Johnson, N. L.

2010-01-01

Since 1964 at least 56 spacecraft and two launch vehicle upper stages have been deliberately fragmented while in Earth orbit. Many of these events have had no long-lasting effects on the near-Earth space environment, but one represents the most devastating satellite breakup in history that will pose hazards to operational spacecraft in low Earth orbit for decades to come. International space debris mitigation guidelines now call for avoiding the creation of long-lived debris from intentional satellite fragmentations. This paper summarizes the reasons for and environmental consequences of deliberate satellite fragmentations. Contrary to popular belief, only one in five deliberate fragmentations have been related to the testing of anti-satellite weapon systems, for which only one such test has occurred during the past 25 years. Other reasons for deliberate satellite fragmentations range from engineering tests to protecting national security information. Whereas the majority of deliberate satellite fragmentations have occurred in low Earth orbits, some have involved spacecraft in highly elliptical orbits. The former Soviet Union and the current Russian Federation have been responsible for 90% of all identified deliberate on-orbit satellite fragmentations.

Increasing cellular coverage within integrated terrestrial/satellite mobile networks

NASA Technical Reports Server (NTRS)

Castro, Jonathan P.

1995-01-01

When applying the hierarchical cellular concept, the satellite acts as giant umbrella cell covering a region with some terrestrial cells. If a mobile terminal traversing the region arrives to the border-line or limits of a regular cellular ground service, network transition occurs and the satellite system continues the mobile coverage. To adequately assess the boundaries of service of a mobile satellite system an a cellular network within an integrated environment, this paper provides an optimized scheme to predict when a network transition may be necessary. Under the assumption of a classified propagation phenomenon and Lognormal shadowing, the study applies an analytical approach to estimate the location of a mobile terminal based on a reception of the signal strength emitted by a base station.

Altered Functional Properties of Satellite Glial Cells in Compressed Spinal Ganglia

PubMed Central

Zhang, Haijun; Mei, Xiaofeng; Zhang, Pu; Ma, Chao; White, Fletcher A; Donnelly, David F; LaMotte, Robert H

2009-01-01

The cell bodies of sensory neurons in the dorsal root ganglion (DRG) are enveloped by satellite glial cells (SGCs). In an animal model of intervertebral foraminal stenosis and low-back pain, a chronic compression of the DRG (CCD) increases the excitability of neuronal cell bodies in the compressed ganglion. The morphological and electrophysiological properties of SGCs were investigated in both CCD and uninjured, control lumbar DRGs. SGCs responded within 12 hours of the onset of CCD as indicated by an increased expression of glial fibrillary acidic protein (GFAP) in the compressed DRG but to lesser extent in neighboring or contralateral DRGs. Within one week, coupling through gap junctions between SGCs was significantly enhanced in the compressed ganglion. Under whole-cell patch clamp recordings, inward and outward potassium currents, but not sodium currents, were detected in individual SGCs. SGCs enveloping differently sized neurons had similar electrophysiological properties. SGCs in the compressed vs. control DRG exhibited significantly reduced inwardly rectifying potassium currents (Kir), increased input resistances and positively shifted resting membrane potentials. The reduction in Kir was greater for nociceptive medium-sized neurons compared to non-nociceptive neurons. Kir currents of SGCs around spontaneously active neurons were significantly reduced one day after compression but recovered by 7 days. These data demonstrate rapid alterations in glial membrane currents and GFAP expression in close temporal association with the development of neuronal hyperexcitability in the CCD model of europathic pain. However, these alterations are not fully sustained and suggest other mechanisms for the maintenance of the hyperexcitable state. PMID:19330845

Determination of motion extrema in multi-satellite systems

NASA Astrophysics Data System (ADS)

Allgeier, Shawn E.

Spacecraft, or satellite formation flight has been a topic of interest dating back to the Gemini program of the 1960s. Traditionally space missions have been designed around large monolithic assets. Recent interest in low cost, rapid call up mission architectures structured around fractionated systems, small satellites, and constellations has spurred renewed efforts in spacecraft relative motion problems. While such fractionated, or multi-body systems may provide benefits in terms of risk mitigation and cost savings, they introduce new technical challenges in terms of satellite coordination. Characterization of satellite formations is a vital requirement for them to have utility to industry and government entities. Satellite formations introduce challenges in the form of constellation maintenance, inter-satellite communications, and the demand for more sophisticated guidance, navigation, and control systems. At the core of these challenges is the orbital mechanics which govern the resulting motion. New applications of algebraic techniques are applied to the formation flight problem, specifically Gröbner basis tools, as a means of determining extrema of certain quantities pertaining to formation flight. Specifically, bounds are calculated for the relative position components, relative speed, relative velocity components, and range rate. The position based metrics are relevant for planning formation geometry, particularly in constellation or Earth observation applications. The velocity metrics are relevant in the design of end game interactions for rendezvous and proximity operations. The range rate of one satellite to another is essential in the design of radio frequency hardware for inter-satellite communications so that the doppler shift can be calculated a priori. Range rate may also have utility in space based surveillance and space situational awareness concerns, such as cross tagging. The results presented constitute a geometric perspective and have utility

Mixed lactate and caffeine compound increases satellite cell activity and anabolic signals for muscle hypertrophy.

PubMed

Oishi, Yoshimi; Tsukamoto, Hayato; Yokokawa, Takumi; Hirotsu, Keisuke; Shimazu, Mariko; Uchida, Kenji; Tomi, Hironori; Higashida, Kazuhiko; Iwanaka, Nobumasa; Hashimoto, Takeshi

2015-03-15

We examined whether a mixed lactate and caffeine compound (LC) could effectively elicit proliferation and differentiation of satellite cells or activate anabolic signals in skeletal muscles. We cultured C2C12 cells with either lactate or LC for 6 h. We found that lactate significantly increased myogenin and follistatin protein levels and phosphorylation of P70S6K while decreasing the levels of myostatin relative to the control. LC significantly increased protein levels of Pax7, MyoD, and Ki67 in addition to myogenin, relative to control. LC also significantly increased follistatin expression relative to control and stimulated phosphorylation of mTOR and P70S6K. In an in vivo study, male F344/DuCrlCrlj rats were assigned to control (Sed, n = 10), exercise (Ex, n = 12), and LC supplementation (LCEx, n = 13) groups. LC was orally administered daily. The LCEx and Ex groups were exercised on a treadmill, running for 30 min at low intensity every other day for 4 wk. The LCEx group experienced a significant increase in the mass of the gastrocnemius (GA) and tibialis anterior (TA) relative to both the Sed and Ex groups. Furthermore, the LCEx group showed a significant increase in the total DNA content of TA compared with the Sed group. The LCEx group experienced a significant increase in myogenin and follistatin expression of GA relative to the Ex group. These results suggest that administration of LC can effectively increase muscle mass concomitant with elevated numbers of myonuclei, even with low-intensity exercise training, via activated satellite cells and anabolic signals. Copyright © 2015 the American Physiological Society.

NASA standard GAS Can satellite. [Get-Away Special canister for STS Orbiter

NASA Technical Reports Server (NTRS)

Cudmore, Patrick H.; Mcintosh, W.; Edison, M.; Nichols, S.; Mercier, E.

1989-01-01

The Get-Away Special canister (GAS Can) satellite is a small, (150 lb) low-cost satellite making it possible for commercial and scientific institutions to conduct experiments in space on an economical and short-term basis. The current model is called Xsat (Exceptional Satellite) and is designed to be launched from a GAS canister on the STS Orbiter; also provided is a low-cost automated PC-operated ground station for commercial, scientific, and government users. The Xsat structure is diagrammed, and details such as payload interface, weight restrictions, and structural loads are described in detail, pointing out that Xsat has a maximum payload weight of 50 lbs, and has a natural vibration frequency of around 45 Hz, with a minimum requiremet of 35 Hz. Thermal designs, power system, electronics, computer design and bus system, and satellite operations are all outlined.

Open-cell and closed-cell clouds off Peru

NASA Image and Video Library

2010-04-27

2010/107 - 04/17 at 21 :05 UTC. Open-cell and closed-cell clouds off Peru, Pacific Ocean Resembling a frosted window on a cold winter's day, this lacy pattern of marine clouds was captured off the coast of Peru in the Pacific Ocean by the MODIS on the Aqua satellite on April 19, 2010. The image reveals both open- and closed-cell cumulus cloud patterns. These cells, or parcels of air, often occur in roughly hexagonal arrays in a layer of fluid (the atmosphere often behaves like a fluid) that begins to "boil," or convect, due to heating at the base or cooling at the top of the layer. In "closed" cells warm air is rising in the center, and sinking around the edges, so clouds appear in cell centers, but evaporate around cell edges. This produces cloud formations like those that dominate the lower left. The reverse flow can also occur: air can sink in the center of the cell and rise at the edge. This process is called "open cell" convection, and clouds form at cell edges around open centers, which creates a lacy, hollow-looking pattern like the clouds in the upper right. Closed and open cell convection represent two stable atmospheric configurations — two sides of the convection coin. But what determines which path the "boiling" atmosphere will take? Apparently the process is highly chaotic, and there appears to be no way to predict whether convection will result in open or closed cells. Indeed, the atmosphere may sometimes flip between one mode and another in no predictable pattern. Satellite: Aqua NASA/GSFC/Jeff Schmaltz/MODIS Land Rapid Response Team To learn more about MODIS go to: rapidfire.sci.gsfc.nasa.gov/gallery/?latest NASA Goddard Space Flight Center is home to the nation's largest organization of combined scientists, engineers and technologists that build spacecraft, instruments and new technology to study the Earth, the sun, our solar system, and the universe.

Application of the Iridium Satellite System to Aeronautical Communications

NASA Technical Reports Server (NTRS)

Kerczewski, Robert J.; Meza, Mike; Gupta, Om

2008-01-01

The next generation air transportation system will require greater air-ground communications capacity to accommodate more air traffic with increased safety and efficiency. Communications will remain primarily terrestrially based, but satellite communications will have an increased role. Inmarsat s aeronautical services have been approved and are in use for aeronautical safety communications provided by geostationary satellites. More recently the approval process for the Iridium low earth orbit constellation is nearing completion. The current Iridium system will be able to provide basic air traffic services communications suitable for oceanic, remote and polar regions. The planned second generation of the Iridium system, called Iridium NEXT, will provide enhanced capabilities and enable a greater role in the future of aeronautical communications. This paper will review the potential role of satellite communications in the future of air transportation, the Iridium approval process and relevant system testing, and the potential role of Iridium NEXT.

Analytic studies on satellite detection of severe, two-cell tornadoes

NASA Technical Reports Server (NTRS)

Carrier, G. F.; Dergarabedian, P.; Fendell, F. E.

1979-01-01

From funnel-cloud-length interpretation, the severe tornado is characterized by peak swirl speed relative to the axis of rotation of about 90 m/s. Thermohydrodynamic achievement of the pressure deficit from ambient necessary to sustain such swirls requires that a dry, compressionally heated, non-rotating downdraft of initially tropopause-level air lie within an annulus of rapidly swirling, originally low-level air ascending on a near-moist-adiabatic locus of thermodynamic states. The two-cell structure furnishes an observable parameter possibly accessible to a passively instrumented, geosynchronous meteorological satellite with mesoscale resolution, for early detection of a severe tornado. Accordingly, the low-level turnaround region, in which the surface inflow layer separates to become a free ascending layer and for which inviscid modeling suffices, is examined quantitatively. Preliminary results indicate that swirl overshoot, i.e., swirl speeds in the turnaround region in excess of the maximum achieved in the potential vortex, is modest.

Exploring NASA Satellite Data with High Resolution Visualization

NASA Astrophysics Data System (ADS)

Wei, J. C.; Yang, W.; Johnson, J. E.; Shen, S.; Zhao, P.; Gerasimov, I. V.; Vollmer, B.; Vicente, G. A.; Pham, L.

2013-12-01

Satellite data products are important for a wide variety of applications that can bring far-reaching benefits to the science community and the broader society. These benefits can best be achieved if the satellite data are well utilized and interpreted, such as model inputs from satellite, or extreme event (such as volcano eruption, dust storm, ...etc) interpretation from satellite. Unfortunately, this is not always the case, despite the abundance and relative maturity of numerous satellite data products provided by NASA and other organizations. Such obstacles may be avoided by providing satellite data as ';Images' with accurate pixel-level (Level 2) information, including pixel coverage area delineation and science team recommended quality screening for individual geophysical parameters. We will present a prototype service from the Goddard Earth Sciences Data and Information Services Center (GES DISC) supporting various visualization and data accessing capabilities from satellite Level 2 data (non-aggregated and un-gridded) at high spatial resolution. Functionality will include selecting data sources (e.g., multiple parameters under the same measurement, like NO2 and SO2 from Ozone Monitoring Instrument (OMI), or same parameter with different methods of aggregation, like NO2 in OMNO2G and OMNO2D products), defining area-of-interest and temporal extents, zooming, panning, overlaying, sliding, and data subsetting and reformatting. The portal interface will connect to the backend services with OGC standard-compliant Web Mapping Service (WMS) and Web Coverage Service (WCS) calls. The interface will also be able to connect to other OGC WMS and WCS servers, which will greatly enhance its expandability to integrate additional outside data/map sources.

Satellited 4q identified in amniotic fluid cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, I.; Hsieh, C.L.; Songster, G.

Extra material was identified on the distal long arm of a chromosome 4 in an amniotic fluid specimen sampled at 16.6 weeks of gestational age. There was no visible loss of material from chromosome 4, and no evidence for a balanced rearrangement. The primary counseling issue in this case was advanced maternal age. Ultrasound findings were normal, and family history was unremarkable. The identical 4qs chromosome was observed in cells from a paternal peripheral blood specimen and appeared to be an unbalanced rearrangement. This extra material was NOR positive in lymphocytes from the father, but was negative in the fetalmore » amniocytes. Father`s relatives were studied to verify the familial origin of this anomaly. In situ hybridization with both exon and intron sequences of ribosomal DNA demonstrated that ribosomal DNA is present at the terminus of the 4qs chromosome in the fetus, father, and paternal grandmother. This satellited 4q might have been derived from a translocation event that resulted in very little or no loss from the 4q and no specific phenotype. This derivative chromosome 4 has been inherited through at least 3 generations of phenotypically normal individuals. 8 refs., 3 figs.« less

H2@Scale Laboratory CRADA Call | Hydrogen and Fuel Cells | NREL

Science.gov Websites

research and development agreement (CRADA) projects with the Hydrogen at Scale (H2@Scale) national CRADA Call H2@Scale CRADA Agreement Template Responses to CRADA Call Questions: Part 1 (includes

Power attenuation characteristics as switch-over criterion in personal satellite mobile communications

NASA Technical Reports Server (NTRS)

Castro, Jonathan P.

1993-01-01

A third generation mobile system intends to support communications in all environments (i.e., outdoors, indoors at home or office and when moving). This system will integrate services that are now available in architectures such as cellular, cordless, mobile data networks, paging, including satellite services to rural areas. One way through which service integration will be made possible is by supporting a hierarchical cellular structure based on umbrella cells, macro cells, micro and pico cells. In this type of structure, satellites are part of the giant umbrella cells allowing continuous global coverage, the other cells belong to cities, neighborhoods, and buildings respectively. This does not necessarily imply that network operation of terrestrial and satellite segments interconnect to enable roaming and spectrum sharing. However, the cell concept does imply hand-off between different cell types, which may involve change of frequency. Within this propsective, the present work uses power attenuation characteristics to determine a dynamic criterion that allows smooth transition from space to terrestrial networks. The analysis includes a hybrid channel that combines Rician, Raleigh and Log Normal fading characteristics.

An MIP model to schedule the call center workforce and organize the breaks

NASA Astrophysics Data System (ADS)

Türker, Turgay; Demiriz, Ayhan

2016-06-01

In modern economies, companies place a premium on managing their workforce efficiently especially in labor intensive service sector, since the services have become the significant portion of the economies. Tour scheduling is an important tool to minimize the overall workforce costs while satisfying the minimum service level constraints. In this study, we consider the workforce management problem of an inbound call-center while satisfying the call demand within the short time periods with the minimum cost. We propose a mixed-integer programming model to assign workers to the daily shifts, to determine the weekly off-days, and to determine the timings of lunch and other daily breaks for each worker. The proposed model has been verified on the weekly demand data observed at a specific call center location of a satellite TV operator. The model was run on both 15 and 10 minutes demand estimation periods (planning time intervals).

Open-cell and closed-cell clouds off Peru [detail

NASA Image and Video Library

2017-12-08

2010/107 - 04/17 at 21 :05 UTC. Open-cell and closed-cell clouds off Peru, Pacific Ocean. To view the full fame of this image to go: www.flickr.com/photos/gsfc/4557497219/ Resembling a frosted window on a cold winter's day, this lacy pattern of marine clouds was captured off the coast of Peru in the Pacific Ocean by the MODIS on the Aqua satellite on April 19, 2010. The image reveals both open- and closed-cell cumulus cloud patterns. These cells, or parcels of air, often occur in roughly hexagonal arrays in a layer of fluid (the atmosphere often behaves like a fluid) that begins to "boil," or convect, due to heating at the base or cooling at the top of the layer. In "closed" cells warm air is rising in the center, and sinking around the edges, so clouds appear in cell centers, but evaporate around cell edges. This produces cloud formations like those that dominate the lower left. The reverse flow can also occur: air can sink in the center of the cell and rise at the edge. This process is called "open cell" convection, and clouds form at cell edges around open centers, which creates a lacy, hollow-looking pattern like the clouds in the upper right. Closed and open cell convection represent two stable atmospheric configurations — two sides of the convection coin. But what determines which path the "boiling" atmosphere will take? Apparently the process is highly chaotic, and there appears to be no way to predict whether convection will result in open or closed cells. Indeed, the atmosphere may sometimes flip between one mode and another in no predictable pattern. Satellite: Aqua NASA/GSFC/Jeff Schmaltz/MODIS Land Rapid Response Team To learn more about MODIS go to: rapidfire.sci.gsfc.nasa.gov/gallery/?latest NASA Goddard Space Flight Center is home to the nation's largest organization of combined scientists, engineers and technologists that build spacecraft, instruments and new technology to study the Earth, the sun, our solar system, and the universe.

Enhanced AIS receiver design for satellite reception

NASA Astrophysics Data System (ADS)

Clazzer, Federico; Lázaro, Francisco; Plass, Simon

2016-12-01

The possibility to detect Automatic Identification System (AIS) messages from low earth orbit (LEO) satellites paves the road for a plurality of new and unexplored services. Besides worldwide tracking of vessels, maritime traffic monitoring, analysis of vessel routes employing big data, and oceans monitoring are just few of the fields, where satellite-aided AIS is beneficial. Designed for ship-to-ship communication and collision avoidance, AIS satellite reception performs poorly in regions with a high density of vessels. This calls for the development of advanced satellite AIS receivers able to improve the decoding capabilities. In this context, our contribution focuses on the introduction of a new enhanced AIS receiver design and its performance evaluation. The enhanced receiver makes use of a coherent receiver for the low signal-to-noise ratio (SNR) region, while for medium to high SNRs, a differential Viterbi receiver is used. Additional novelty of our work is in the exploitation of previously decoded packets from one vessel that is still under the LEO reception range, to improve the vessel detection probability. The assessment of the performance against a common receiver is done making the use of a simple and tight model of the medium access (MAC) layer and the multi-packet reception (MPR) matrix for physical layer (PHY) representation. Performance results show the benefits of such enhanced receiver, especially when it is bundled with successive interference cancellation (SIC).

Effect of TCEA3 on the differentiation of bovine skeletal muscle satellite cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhu, Yue; Tong, Hui-Li; Li, Shu-Feng

Bovine muscle-derived satellite cells (MDSCs) are important for animal growth. In this study, the effect of transcription elongation factor A3 (TCEA3) on bovine MDSC differentiation was investigated. Western blotting, immunofluorescence assays, and cytoplasmic and nuclear protein isolation and purification techniques were used to determine the expression pattern and protein localization of TCEA3 in bovine MDSCs during in vitro differentiation. TCEA3 expression was upregulated using the CRISPR/Cas9 technique to study its effects on MDSC differentiation in vitro. TCEA3 expression gradually increased during the in vitro differentiation of bovine MDSCs and peaked on the 5th day of differentiation. TCEA3 was mainly localized in the cytoplasmmore » of bovine MDSCs, and its expression was not detected in the nucleus. The level of TCEA3 was relatively higher in myotubes at a higher degree of differentiation than during early differentiation. After transfection with a TCEA3-activating plasmid vector (TCEA3 overexpression) for 24 h, the myotube fusion rate, number of myotubes, and expression levels of the muscle differentiation-related loci myogenin (MYOG) and myosin heavy chain 3 (MYH3) increased significantly during the in vitro differentiation of bovine MDSCs. After transfection with a TCEA3-inhibiting plasmid vector for 24 h, the myotube fusion rate, number of myotubes, and expression levels of MYOG and MYH3 decreased significantly. Our results indicated, for the first time, that TCEA3 promotes the differentiation of bovine MDSCs and have implications for meat production and animal rearing. - Highlights: • Muscle-derived satellite cell differentiation is promoted by TCEA3. • TCEA3 protein was localized in the cytoplasm, but not nuclei of bovine MDSCs. • TCEA3 levels increased as myotube differentiation increased. • TCEA3 affected myotube fusion, myotube counts, and MYOG and MYH3 levels.« less

Mentoring for Youth in Schools and Communities. National Satellite Videoconference. [Videotape].

ERIC Educational Resources Information Center

Department of Justice, Washington, DC. Office of Juvenile Justice and Delinquency Prevention.

This videotape presents the National Satellite Videoconference on mentoring for youth. The video opens with a discussion of mentoring and presents panelists who make statements about mentoring and youth programs and respond to questions called in by videoconference participants at approximately 500 downlinked sites. Panelists were: (1) Shay…

Changes in proteolytic enzymes mRNAs and proteins relevant for meat quality during myogenesis and hypoxia of primary bovine satellite cells.

PubMed

Yang, You Bing; Pandurangan, Muthuraman; Hwang, InHo

2012-06-01

The current study was conducted to evaluate the functions of μ-calpain (CAPN1), calpastatin, HSPs (heat shock proteins), and caspases during myogenesis and cell death induced by sodium azide (NaN(3)) hypoxia. The cell samples were divided into three groups: satellite cells formed at confluent monolayer (stage 1), stage 1 cells fusion into myotubes on d eight post-differentiation (stage 2), and stage 2 cells treated with 1 mM NaN(3) for 24 h (stage 3). Real-time RT-PCR showed that stage 2 cells had increased CAPN1, calpastatin, caspase 7, and CARD9 (Caspase activation and recruitment domain 9) mRNA expressions compared to stage 1 cells (*p < 0.05). By Western blotting caspase 3, caspase 7, caspase 8, and caspase 9 protein levels increased in cells at stage 2 compared to cells at stage 1 (*p < 0.05). Real-time RT-PCR showed that stage 3 cells had increased CAPN1, calpastatin, caspase 7, HSP70 (70 kDA heat shock proteins), and HSP90 (90 kDA heat shock proteins-alpha) and decreased CARD9 mRNA expression compared to stage 2 cells (*p < 0.05). Stage 3 samples had increase caspase 7 and caspase 12 activities compared to stage 2 samples, and by Western blotting protein levels of both HSP70 and HSP90 expressions, increased significantly under hypoxia condition (*p < 0.05). Here, we conclude that CAPN1, calpastatin, caspase 3, caspase 7, caspase 8, and CARD9 have important roles for satellite cell myogenesis; and that caspase 7, 12, HSP70, and HSP90 are involved in the process of apoptotic cell death under hypoxia conditions and we speculate that these proteins may be involved in early postmortem proteolysis and meat tenderization.

Implementation of a system to provide mobile satellite services in North America

NASA Technical Reports Server (NTRS)

Johanson, Gary A.; Davies, N. George; Tisdale, William R. H.

1993-01-01

This paper describes the implementation of the ground network to support Mobile Satellite Services (MSS). The system is designed to take advantage of a powerful new satellite series and provides significant improvements in capacity and throughput over systems in service today. The system is described in terms of the services provided and the system architecture being implemented to deliver those services. The system operation is described including examples of a circuit switched and packet switched call placement. The physical architecture is presented showing the major hardware components and software functionality placement within the hardware.

Very long baseline interferometry using a communication satellite

NASA Technical Reports Server (NTRS)

Swenson, G. W., Jr.

1975-01-01

A planned experiment is discussed in long-baseline interferometry, using the Communications Technology Satellite to transmit the base-band signal from one telescope to another for real-time correlation. A 20 megabit data rate is planned, calling for a delay-line of 10 MHz bandwidth and controllable delay up to 275 milliseconds. A number of sources will be studied on baselines from Ontario to West Virginia and California.

COUP-TFII regulates satellite cell function and muscular dystrophy.

PubMed

Xie, Xin; Tsai, Sophia Y; Tsai, Ming-Jer

2016-10-03

Duchenne muscular dystrophy (DMD) is a severe and progressive muscle-wasting disease caused by mutations in the dystrophin gene. Although dystrophin deficiency in myofiber triggers the disease's pathological changes, the degree of satellite cell (SC) dysfunction defines disease progression. Here, we have identified chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) hyperactivity as a contributing factor underlying muscular dystrophy in a dystrophin-deficient murine model of DMD. Ectopic expression of COUP-TFII in murine SCs led to Duchenne-like dystrophy in the muscles of control animals and exacerbated degenerative myopathies in dystrophin-deficient mice. COUP-TFII-overexpressing mice exhibited regenerative failure that was attributed to deficient SC proliferation and myoblast fusion. Mechanistically, we determined that COUP-TFII coordinated a regenerative program through combined regulation of multiple promyogenic factors. Furthermore, inhibition of COUP-TFII preserved SC function and counteracted the muscle weakness associated with Duchenne-like dystrophy in the murine model, suggesting that targeting COUP-TFII is a potential treatment for DMD. Together, our findings reveal a regulatory role of COUP-TFII in the development of muscular dystrophy and open up a potential therapeutic opportunity for managing disease progression in patients with DMD.

Ultrastructure and composition of Call-Exner bodies in bovine follicles.

PubMed

van Wezel, I L; Irving-Rodgers, H F; Sado, Y; Ninomiya, Y; Rodgers, R J

1999-05-01

Call-Exner bodies are present in ovarian follicles of a range of species including human and rabbit, and in a range of human ovarian tumors. We have also found structures resembling Call-Exner bodies in bovine preantral and small antral follicles. Hematoxylin and eosin staining of single sections of bovine ovaries has shown that 30% of preantral follicles with more than one layer of granulosa cells and 45% of small (less than 650 microns) antral follicles have at least one Call-Exner body composed of a spherical eosinophilic region surrounded by a rosette of granulosa cells. Alcian blue stains the spherical eosinophilic region of the Call-Exner bodies. Electron microscopy has demonstrated that some Call-Exner bodies contain large aggregates of convoluted basal lamina, whereas others also contain regions of unassembled basal-lamina-like material. Individual chains of the basal lamina components type IV collagen (alpha 1 to alpha 5) and laminin (alpha 1, beta 2 and delta 1) have been immunolocalized to Call-Exner bodies in sections of fresh-frozen ovaries. Bovine Call-Exner bodies are presumably analogous to Call-Exner bodies in other species but are predominantly found in preantral and small antral follicles, rather than large antral follicles. With follicular development, the basal laminae of Call-Exner bodies change in their apparent ratio of type IV collagen to laminin, similar to changes observed in the follicular basal lamina, suggesting that these structures have a common cellular origin.

Isolation, characterization, and molecular regulation of muscle stem cells

PubMed Central

Fukada, So-ichiro; Ma, Yuran; Ohtani, Takuji; Watanabe, Yoko; Murakami, Satoshi; Yamaguchi, Masahiko

2013-01-01

Skeletal muscle has great regenerative capacity which is dependent on muscle stem cells, also known as satellite cells. A loss of satellite cells and/or their function impairs skeletal muscle regeneration and leads to a loss of skeletal muscle power; therefore, the molecular mechanisms for maintaining satellite cells in a quiescent and undifferentiated state are of great interest in skeletal muscle biology. Many studies have demonstrated proteins expressed by satellite cells, including Pax7, M-cadherin, Cxcr4, syndecan3/4, and c-met. To further characterize satellite cells, we established a method to directly isolate satellite cells using a monoclonal antibody, SM/C-2.6. Using SM/C-2.6 and microarrays, we measured the genes expressed in quiescent satellite cells and demonstrated that Hesr3 may complement Hesr1 in generating quiescent satellite cells. Although Hesr1- or Hesr3-single knockout mice show a normal skeletal muscle phenotype, including satellite cells, Hesr1/Hesr3-double knockout mice show a gradual decrease in the number of satellite cells and increase in regenerative defects dependent on satellite cell numbers. We also observed that a mouse's genetic background affects the regenerative capacity of its skeletal muscle and have established a line of DBA/2-background mdx mice that has a much more severe phenotype than the frequently used C57BL/10-mdx mice. The phenotype of DBA/2-mdx mice also seems to depend on the function of satellite cells. In this review, we summarize the methodology of direct isolation, characterization, and molecular regulation of satellite cells based on our results. The relationship between the regenerative capacity of satellite cells and progression of muscular disorders is also summarized. In the last part, we discuss application of the accumulating scientific information on satellite cells to treatment of patients with muscular disorders. PMID:24273513

Regulatory T cells and skeletal muscle regeneration.

PubMed

Schiaffino, Stefano; Pereira, Marcelo G; Ciciliot, Stefano; Rovere-Querini, Patrizia

2017-02-01

Skeletal muscle regeneration results from the activation and differentiation of myogenic stem cells, called satellite cells, located beneath the basal lamina of the muscle fibers. Inflammatory and immune cells have a crucial role in the regeneration process. Acute muscle injury causes an immediate transient wave of neutrophils followed by a more persistent infiltration of M1 (proinflammatory) and M2 (anti-inflammatory/proregenerative) macrophages. New studies show that injured muscle is also infiltrated by a specialized population of regulatory T (Treg) cells, which control both the inflammatory response, by promoting the M1-to-M2 switch, and the activation of satellite cells. Treg cells accumulate in injured muscle in response to specific cytokines, such as IL-33, and promote muscle growth by releasing growth factors, such as amphiregulin. Muscle repair during aging is impaired due to reduced number of Treg cells and can be enhanced by IL-33 supplementation. Migration of Treg cells could also contribute to explain the effect of heterochronic parabiosis, whereby muscle regeneration of aged mice can be improved by a parabiotically linked young partners. In mdx dystrophin-deficient mice, a model of human Duchenne muscular dystrophy, muscle injury, and inflammation is mitigated by expansion of the Treg-cell population but exacerbated by Treg-cell depletion. These findings support the notion that immunological mechanisms are not only essential in the response to pathogenic microbes and tumor cells but also have a wider homeostatic role in tissue repair, and open new perspectives for boosting muscle growth in chronic muscle disease and during aging. © 2016 Federation of European Biochemical Societies.

The growing impact of satellite data in daily life

NASA Astrophysics Data System (ADS)

Stramondo, Salvatore

2015-04-01

Satellite images have a growing role in our daily life. Weather previsions, telecommunications, environmental planning, disaster mitigation and monitoring: these are only some of the fieldworks where space remote sensing data, and related processing techniques, provide extremely useful information to policy/decision makers, scientists, or to the "simple" citizen. The demonstration of the level of attention provided by the International Community to the impact of new technologies and satellite Earth Observation, in particular, onto everyday life is testified by the recent and forthcoming project calls. Horizon 2020, for instance, identified "Societal challenges" and "Science with and for Society" among the main pillars. In sub-themes we may read references to the "Environment", "Secure societies", "Climate changes", and many others, most of which soliciting the use of remote sensing technologies. In such scenario the scientists should be conscious about the capabilities and the implications in applying new technologies. Recent examples might be explanatory. Satellite data properly managed can be used to measure millimetric and/or centimetric movements of buildings and infrastructures. It has been demonstrated how long term monitoring of urban areas detecting pre-collapse deformations might provide useful hints to prevent such dramatic events. Or, in different frameworks, satellite data can be an advanced instrument for intelligence and military purposes. With such premises, ethic issues assume a key role to properly address the use of satellite technologies.

Active Cavity Irradiance Monitor Satellite ACRIMSAT Artist Concept

NASA Image and Video Library

1999-12-21

The Active Cavity Irradiance Monitor Satellite, or ACRIMSAT, mission is a climate change investigation that measures changes in how much of the sun's energy reaches Earth's atmosphere. This energy, called solar irradience, creates winds, heats the land and drives ocean currents, and therefore contains significant data that climatologists can use to improve predictions of climate change and global warming. The satellite's Active Cavity Irradiance Monitor III instrument, now in its third generation, has been used since the 1980s to study solar irradiance and its impacts on global warming. Scientists, using data from the instrument, now theorize that there is a significant correlation between solar radiation and global warming. ACRIMSAT completed its five-year primary mission in 2005 when it began operating under its extended mission. http://photojournal.jpl.nasa.gov/catalog/PIA18157

A sustainable genetic algorithm for satellite resource allocation

NASA Technical Reports Server (NTRS)

Abbott, R. J.; Campbell, M. L.; Krenz, W. C.

1995-01-01

A hybrid genetic algorithm is used to schedule tasks for 8 satellites, which can be modelled as a robot whose task is to retrieve objects from a two dimensional field. The objective is to find a schedule that maximizes the value of objects retrieved. Typical of the real-world tasks to which this corresponds is the scheduling of ground contacts for a communications satellite. An important feature of our application is that the amount of time available for running the scheduler is not necessarily known in advance. This requires that the scheduler produce reasonably good results after a short period but that it also continue to improve its results if allowed to run for a longer period. We satisfy this requirement by developing what we call a sustainable genetic algorithm.

Integrated Satellite Control in REIMEI (INDEX) Satellite

NASA Astrophysics Data System (ADS)

Fukuda, Seisuke; Mizuno, Takahide; Sakai, Shin-Ichiro; Fukushima, Yousuke; Saito, Hirobumi

REIMEI/INDEX (INnovative-technology Demonstration EXperiment) is a 70kg class small satellite which the Institute of Space and Astronautical Science, Japan Exploration Agency, ISAS/JAXA, has developed for observation of auroral small-scale dynamics as well as demonstration of advanced satellite technologies. An important engineering mission of REIMEI is integrated satellite control using commercial RISC CPUs with a triple voting system in order to ensure fault-tolerance against radiation hazards. Software modules concerning every satellite function, such as attitude control, data handling, and mission applications, work cooperatively so that highly sophisticated satellite control can be performed. In this paper, after a concept of the integrated satellite control is introduced, the Integrated Controller Unit (ICU) is described in detail. Also unique topics in developing the integrated control system are shown.

Perl Tools for Automating Satellite Ground Systems

NASA Technical Reports Server (NTRS)

McLean, David; Haar, Therese; McDonald, James

2000-01-01

The freeware scripting language Pert offers many opportunities for automating satellite ground systems for new satellites as well as older, in situ systems. This paper describes a toolkit that has evolved from of the experiences gained by using Pert to automate the ground system for the Compton Gamma Ray Observatory (CGRO) and for automating some of the elements in the Earth Observing System Data and Operations System (EDOS) ground system at Goddard Space Flight Center (GSFC). CGRO is an older ground system that was forced to automate because of fund cuts. Three 8 hour shifts were cut back to one 8 hour shift, 7 days per week. EDOS supports a new mission called Terra, launched December 1999 that requires distribution and tracking of mission-critical reports throughout the world. Both of these ground systems use Pert scripts to process data and display it on the Internet as well as scripts to coordinate many of the other systems that make these ground systems work as a coherent whole. Another task called Automated Multimodal Trend Analysis System (AMTAS) is looking at technology for isolation and recovery of spacecraft problems. This effort has led to prototypes that seek to evaluate various tools and technology that meet at least some of the AMTAS goals. The tools, experiences, and lessons learned by implementing these systems are described here.

Nano-Satellite Avionics

NASA Technical Reports Server (NTRS)

Culver, Harry

1999-01-01

Abstract NASA's Goddard Space Flight Center (GSFC) is currently developing a new class of satellites called the nano-satellite (nano-sat). A major objective of this development effort is to provide the technology required to enable a constellation of tens to hundreds of nano-satellites to make both remote and in-situ measurements from space. The Nano-sat will be a spacecraft weighing a maximum of 10 kg, including the propellant mass, and producing at least 5 Watts of power to operate the spacecraft. The electronics are required to survive a total radiation dose rate of 100 krads for a mission lifetime of two years. There are many unique challenges that must be met in order to develop the avionics for such a spacecraft. The first challenge is to develop an architecture that will operate on the allotted 5 Watts and meet the diverging requirements of multiple missions. This architecture will need to incorporate a multitude of new advanced microelectronic technologies. The microelectronics developed must be a modular and scalable packaging of technology to solve the problem of developing a solution to both reduce cost and meet the requirements of various missions. This development will utilize the most cost effective approach, whether infusing commercially driven semiconductor devices into spacecraft applications or partnering with industry to design and develop low cost, low power, low mass, and high capacity data processing devices. This paper will discuss the nano-sat architecture and the major technologies that will be developed. The major technologies that will be covered include: (1) Light weight Low Power Electronics Packaging, (2) Radiation Hard/Tolerant, Low Power Processing Platforms, (3) High capacity Low Power Memory Systems (4) Radiation Hard reconfiguragble field programmable gate array (rFPGA)

A gravity gradient stabilized solar power satellite design

NASA Technical Reports Server (NTRS)

Bowden, M. L.

1981-01-01

The concept of a solar power satellite (SPS) is reviewed, and a design proposed for such a satellite taking advantage of solar radiation pressure and gravity gradient forces to eliminate much of the structure from the baseline configuration. The SPS design consists of a solar cell array lying in the orbital plane and a free floating mirror above to reflect sunlight down onto it. The structural modes of the solar cell array are analyzed and found to be well within control limitations. Preliminary calculations concerning the free floating mirror and its position-keeping propellant requirements are also performed. A numerical example is presented, which shows that, even in terms of mass only, this configuration is a competitive design when compared to the conventional Department of Energy reference design. Other advantages, such as easier assembly in orbit, lower position-keeping propellant requirements, possibilities for decreasing necessary solar cell area, and longer solar cell life, may make this design superior.

Satellite-tracking and earth-dynamics research programs. [NASA Programs on satellite orbits and satellite ground tracks of geodetic satellites

NASA Technical Reports Server (NTRS)

1974-01-01

Observations and research progress of the Smithsonian Astrophysical Observatory are reported. Satellite tracking networks (ground stations) are discussed and equipment (Baker-Nunn cameras) used to observe the satellites is described. The improvement of the accuracy of a laser ranging system of the ground stations is discussed. Also, research efforts in satellite geodesy (tides, gravity anomalies, plate tectonics) is discussed. The use of data processing for geophysical data is examined, and a data base for the Earth and Ocean Physics Applications Program is proposed. Analytical models of the earth's motion (computerized simulation) are described and the computation (numerical integration and algorithms) of satellite orbits affected by the earth's albedo, using computer techniques, is also considered. Research efforts in the study of the atmosphere are examined (the effect of drag on satellite motion), and models of the atmosphere based on satellite data are described.

Virus Satellites Drive Viral Evolution and Ecology

PubMed Central

Frígols, Belén; Quiles-Puchalt, Nuria; Mir-Sanchis, Ignacio; Donderis, Jorge; Elena, Santiago F.; Buckling, Angus; Novick, Richard P.; Marina, Alberto; Penadés, José R.

2015-01-01

Virus satellites are widespread subcellular entities, present both in eukaryotic and in prokaryotic cells. Their modus vivendi involves parasitism of the life cycle of their inducing helper viruses, which assures their transmission to a new host. However, the evolutionary and ecological implications of satellites on helper viruses remain unclear. Here, using staphylococcal pathogenicity islands (SaPIs) as a model of virus satellites, we experimentally show that helper viruses rapidly evolve resistance to their virus satellites, preventing SaPI proliferation, and SaPIs in turn can readily evolve to overcome phage resistance. Genomic analyses of both these experimentally evolved strains as well as naturally occurring bacteriophages suggest that the SaPIs drive the coexistence of multiple alleles of the phage-coded SaPI inducing genes, as well as sometimes selecting for the absence of the SaPI depressing genes. We report similar (accidental) evolution of resistance to SaPIs in laboratory phages used for Staphylococcus aureus typing and also obtain the same qualitative results in both experimental evolution and phylogenetic studies of Enterococcus faecalis phages and their satellites viruses. In summary, our results suggest that helper and satellite viruses undergo rapid coevolution, which is likely to play a key role in the evolution and ecology of the viruses as well as their prokaryotic hosts. PMID:26495848

A native IP satellite communications system

NASA Astrophysics Data System (ADS)

Koudelka, O.; Schmidt, M.; Ebert, J.; Schlemmer, H.; Kastner-Puschl, S.; Riedler, W.

2004-08-01

≪ In the framework of ESA's ARTES-5 program the Institute of Applied Systems Technology (Joanneum Research) in cooperation with the Department of Communications and Wave Propagation has developed a novel meshed satellite communications system which is optimised for Internet traffic and applications (L*IP—Local Network Interconnection via Satellite Systems Using the IP Protocol Suite). Both symmetrical and asymmetrical connections are supported. Bandwidth on demand and guaranteed quality of service are key features of the system. A novel multi-frequency TDMA access scheme utilises efficient methods of IP encapsulation. In contrast to other solutions it avoids legacy transport network techniques. While the DVB-RCS standard is based on ATM or MPEG transport cells, the solution of the L*IP system uses variable-length cells which reduces the overhead significantly. A flexible and programmable platform based on Linux machines was chosen to allow the easy implementation and adaptation to different standards. This offers the possibility to apply the system not only to satellite communications, but provides seamless integration with terrestrial fixed broadcast wireless access systems. The platform is also an ideal test-bed for a variety of interactive broadband communications systems. The paper describes the system architecture and the key features of the system.

Dynamic Call Admission Control Scheme Based on Predictive User Mobility Behavior for Cellular Networks

NASA Astrophysics Data System (ADS)

Intarasothonchun, Silada; Thipchaksurat, Sakchai; Varakulsiripunth, Ruttikorn; Onozato, Yoshikuni

In this paper, we propose a modified scheme of MSODB and PMS, called Predictive User Mobility Behavior (PUMB) to improve performance of resource reservation and call admission control for cellular networks. This algorithm is proposed in which bandwidth is allocated more efficiently to neighboring cells by key mobility parameters in order to provide QoS guarantees for transferring traffic. The probability is used to form a cluster of cells and the shadow cluster, where a mobile unit is likely to visit. When a mobile unit may change the direction and migrate to the cell that does not belong to its shadow cluster, we can support it by making efficient use of predicted nonconforming call. Concomitantly, to ensure continuity of on-going calls with better utilization of resources, bandwidth is borrowed from predicted nonconforming calls and existing adaptive calls without affecting the minimum QoS guarantees. The performance of the PUMB is demonstrated by simulation results in terms of new call blocking probability, handoff call dropping probability, bandwidth utilization, call successful probability, and overhead message transmission when arrival rate and speed of mobile units are varied. Our results show that PUMB provides the better performances comparing with those of MSODB and PMS under different traffic conditions.

Is alarm calling risky? Marmots avoid calling from risky places.

PubMed

Collier, Travis C; Blumstein, Daniel T; Girod, Lewis; Taylor, Charles E

2010-12-01

Alarm calling is common in many species. A prevalent assumption is that calling puts the vocalizing individual at increased risk of predation. If calling is indeed costly, we need special explanations for its evolution and maintenance. In some, but not all species, callers vocalize away from safety and thus may be exposed to an increased risk of predation. However, for species that emit bouts with one or a few calls, it is often difficult to identify the caller and find the precise location where a call was produced. We analyzed the spatial dynamics of yellow-bellied marmot (Marmota flaviventris) alarm calling using an acoustic localization system to determine the location from which calls were emitted. Marmots almost always called from positions close to the safety of their burrows, and, if they produced more than one alarm call, tended to end their calling bouts closer to safety than they started them. These results suggest that for this species, potential increased predation risk from alarm calling is greatly mitigated and indeed calling may have limited predation costs.

Is alarm calling risky? Marmots avoid calling from risky places

PubMed Central

Collier, Travis C.; Blumstein, Daniel T.; Girod, Lewis; Taylor, Charles E.

2010-01-01

Alarm calling is common in many species. A prevalent assumption is that calling puts the vocalizing individual at increased risk of predation. If calling is indeed costly, we need special explanations for its evolution and maintenance. In some, but not all species, callers vocalize away from safety and thus may be exposed to an increased risk of predation. However, for species that emit bouts with one or a few calls, it is often difficult to identify the caller and find the precise location where a call was produced. We analyzed the spatial dynamics of yellow-bellied marmot (Marmota flaviventris) alarm calling using an acoustic localization system to determine the location from which calls were emitted. Marmots almost always called from positions close to the safety of their burrows, and, if they produced more than one alarm call, tended to end their calling bouts closer to safety than they started them. These results suggest that for this species, potential increased predation risk from alarm calling is greatly mitigated and indeed calling may have limited predation costs. PMID:21116460

Protocol and Topology Issues for Wide-Area Satellite Interconnection of Terrestrial Optical LANs

NASA Astrophysics Data System (ADS)

Parraga, N.

2002-01-01

system for multimedia services. EuroSkyWay uses a GEO satellite with onboard switching. It has its own proprietary protocol stack for data link control (DLC), logical link control (LLC) and layer 3 functions such as resource management, call admission control and authentication. Special attention is paid to the IP interworking with Layer 3 function since IP does not support connection set-up and session protocols, thus proper interworking functions with IP signaling protocols for resource reservation routing such as RSVP, BGP, and ICMP need to be developed. Whereas the EuroSkyWay system is an representative for a meshed topology, DVB-RCS systems have usually star configuration with a central hub station. Different data streams are distinguished by program identifiers (PIDs). Recent proposals aim at the evolution of DVB-RCS towards a fully meshed structure. The paper will also discuss the protocol architecture for interconnect SONET LANs over these systems. Finally, a performance comparison of the different solutions will be given in terms of cell overhead rate and signalling effort for selected scenarios.

Improving Satellite Compatible Microdevices to Study Biology in Space

NASA Technical Reports Server (NTRS)

Kalkus, Trevor; Snyder, Jessica; Paulino-Lima, Ivan; Rothschild, Lynn

2017-01-01

The technology for biology in space lags far behind the gold standard for biological experiments on Earth. To remedy this disparity, the Rothschild lab works on proof of concept, prototyping, and developing of new sensors and devices to further the capabilities of biology research on satellites. One such device is the PowerCell Payload System. One goal for synthetic biology in aiding space travel and colonization is to genetically engineer living cells to produce biochemicals in space. However, such farming in space presupposes bacteria retain their functionality post-launch, bombarded by radiation, and without the 1G of Earth. Our questions is, does a co-culture of cyanobacteria and protein-synthesizing bacteria produce Earth-like yields of target proteins? Is the yield sensitive to variable gravitational forces? To answer these questions, a PowerCell Payload System will spend 1 year aboard the German Aerospace Center's Euglena and Combined Regenerative Organic-food Production In Space (Eu:CROPIS) mission satellite. The PowerCell system is a pair of two 48-well microfluidic cards, each well seeded with bacteria. The system integrates fluidic, thermal, optical, electronic, and control systems to germinate bacteria spores, then measure the protein synthesized for comparison to parallel experiments conducted on the Earth. In developing the PowerCell Payload, we gained insight into the shortcomings of biology experiments on satellites. To address these issues, we have started three new prototyping projects: 1) The development of an extremely stable and radiation resistant cell-free system, allowing for the construction of proteins utilizing only cell components instead of living cells. This can be lyophilized on a substrate, like paper. (2) Using paper as a microfluidic platform that is flexible, stable, cheap, and wicking. The capillary action eliminates the need for pumps, reducing volume, mass, and potential failing points. Electrodes can be printed on the paper to

Evidence That Calls-Based and Mobility Networks Are Isomorphic

PubMed Central

Coscia, Michele; Hausmann, Ricardo

2015-01-01

Social relations involve both face-to-face interaction as well as telecommunications. We can observe the geography of phone calls and of the mobility of cell phones in space. These two phenomena can be described as networks of connections between different points in space. We use a dataset that includes billions of phone calls made in Colombia during a six-month period. We draw the two networks and find that the call-based network resembles a higher order aggregation of the mobility network and that both are isomorphic except for a higher spatial decay coefficient of the mobility network relative to the call-based network: when we discount distance effects on the call connections with the same decay observed for mobility connections, the two networks are virtually indistinguishable. PMID:26713730

Satellite rainfall retrieval by logistic regression

NASA Technical Reports Server (NTRS)

Chiu, Long S.

1986-01-01

The potential use of logistic regression in rainfall estimation from satellite measurements is investigated. Satellite measurements provide covariate information in terms of radiances from different remote sensors.The logistic regression technique can effectively accommodate many covariates and test their significance in the estimation. The outcome from the logistical model is the probability that the rainrate of a satellite pixel is above a certain threshold. By varying the thresholds, a rainrate histogram can be obtained, from which the mean and the variant can be estimated. A logistical model is developed and applied to rainfall data collected during GATE, using as covariates the fractional rain area and a radiance measurement which is deduced from a microwave temperature-rainrate relation. It is demonstrated that the fractional rain area is an important covariate in the model, consistent with the use of the so-called Area Time Integral in estimating total rain volume in other studies. To calibrate the logistical model, simulated rain fields generated by rainfield models with prescribed parameters are needed. A stringent test of the logistical model is its ability to recover the prescribed parameters of simulated rain fields. A rain field simulation model which preserves the fractional rain area and lognormality of rainrates as found in GATE is developed. A stochastic regression model of branching and immigration whose solutions are lognormally distributed in some asymptotic limits has also been developed.

NOAA budget would boost satellite funding but cut some key areas

NASA Astrophysics Data System (ADS)

Showstack, Randy

2012-03-01

The White House's proposed fiscal year (FY) 2013 budget for the National Oceanic and Atmospheric Administration (NOAA), announced on 13 February, looks favorable at first glance. The administration's request calls for $5.1 billion, an increase of $153 million (3.1%) above the FY 2012 estimated budget. However, the increase for NOAA satellites is $163 million, which means that other areas within the agency would be slated for decreased funding, including programs within the National Ocean Service (NOS), National Marine Fisheries Service (NMFS), National Weather Service (NWS), and some NOAA education programs. The proposed overall budget for the agency “reflects the overarching importance of weather satellites to public safety, to national security, and to the economy,” NOAA director Jane Lubchenco said at a 16 February briefing, noting that difficult choices were made regarding the budget. “Due to significant resources required for our weather satellites and the economic conditions in the country, other parts of our budget have been reduced, in some cases quite significantly,” she said. She added that the imperative to fund both the Joint Polar Satellite System (JPSS) and geostationary satellites in FY 2013 “imposes serious constraints on the rest of NOAA's budget.”

Defining the transcriptional signature of skeletal muscle stem cells.

PubMed

Yablonka-Reuveni, Z; Day, K; Vine, A; Shefer, G

2008-04-01

Satellite cells, the main source of myoblasts in postnatal muscle, are located beneath the myofiber basal lamina. The myogenic potential of satellite cells was initially documented based on their capacity to produce progeny that fused into myotubes. More recently, molecular markers of resident satellite cells were identified, further contributing to defining these cells as myogenic stem cells that produce differentiating progeny and self-renew. Herein, we discuss aspects of the satellite cell transcriptional milieu that have been intensively investigated in our research. We elaborate on the expression patterns of the paired box (Pax) transcription factors Pax3 and Pax7, and on the myogenic regulatory factors myogenic factor 5 (Myf5), myogenic determination factor 1 (MyoD), and myogenin. We also introduce original data on MyoD upregulation in newly activated satellite cells, which precedes the first round of cell proliferation. Such MyoD upregulation occurred even when parent myofibers with their associated satellite cells were exposed to pharmacological inhibitors of hepatocyte growth factor and fibroblast growth factor receptors, which are typically involved in promoting satellite cell proliferation. These observations support the hypothesis that most satellite cells in adult muscle are committed to rapidly entering myogenesis. We also detected expression of serum response factor in resident satellite cells prior to MyoD expression, which may facilitate the rapid upregulation of MyoD. Aspects of satellite cell self-renewal based on the reemergence of cells expressing Pax7, but not MyoD, in myogenic cultures are discussed further herein. We conclude by describing our recent studies using transgenic mice in which satellite cells are traced and isolated based on their expression of green fluorescence protein driven by regulatory elements of the nestin promoter (nestin-green fluorescence protein). This feature provides us with a novel means of studying satellite cell

Solar power satellite

NASA Technical Reports Server (NTRS)

Davis, H. P.

1978-01-01

The solar power satellite (SPS) concept, under evaluation by NASA since 1974, is discussed. A typical system providing a total of 10,000 MW of electrical power to the ground receiving stations is considered. Energy conversion systems, including the photovoltaic device category using single-crystal silicon cells, are taken into account, as are the 2.45-GHz microwave power-transmission link and the ground receiver (or rectenna). Concepts involving space construction of the satellite's large structures (5 x 25 km) are described, noting that a process similar to the familiar roll-forming of light sheet metal parts has been adapted to the space environment. Transportation vehicles are discussed, including the Space Shuttle planned to reach 60 flights per year by the mid 1980's. Electrical power forecasts and advanced systems cost projections are analyzed, together with a description of costs estimates. The indirect economics of energy research and development, and the present NASA/DOE SPS program are noted.

Optimization of orbital assignment and specification of service areas in satellite communications

NASA Technical Reports Server (NTRS)

Wang, Cou-Way; Levis, Curt A.; Buyukdura, O. Merih

1987-01-01

The mathematical nature of the orbital and frequency assignment problem for communications satellites is explored, and it is shown that choosing the correct permutations of the orbit locations and frequency assignments is an important step in arriving at values which satisfy the signal-quality requirements. Two methods are proposed to achieve better spectrum/orbit utilization. The first, called the delta S concept, leads to orbital assignment solutions via either mixed-integer or restricted basis entry linear programming techniques; the method guarantees good single-entry carrier-to-interference ratio results. In the second, a basis for specifying service areas is proposed for the Fixed Satellite Service. It is suggested that service areas should be specified according to the communications-demand density in conjunction with the delta S concept in order to enable the system planner to specify more satellites and provide more communications supply.

Circumnutations of sunflower hypocotyls in satellite orbit

NASA Technical Reports Server (NTRS)

Brown, A. H.; Chapman, D. K.; Lewis, R. F.; Venditti, A. L.

1990-01-01

The principal objective of the research reported here was to determine whether a plant's periodic growth oscillations, called circumnutations, would persist in the absence of a significant gravitational or inertial force. The definitive experiment was made possible by access to the condition of protracted near weightlessness in an earth satellite. The experiment, performed during the first flight of Spacelab on the National Aeronautics and Space Administration shuttle, Columbia, in November and December, 1983, tested a biophysical model, proposed in 1967, that might account for circumnutation as a gravity-dependent growth response. However, circumnutations were observed in microgravity. They continued for many hours without stimulation by a significant g-force. Therefore, neither a gravitational nor an inertial g-force was an absolute requirement for initiation [correction of initation] or continuation of circumnutation. On average, circumnutation was significantly more vigorous in satellite orbit than on earth-based clinostats. Therefore, at least for sunflower (Helianthus annuus L.) circumnutation, clinostatting is not the functional equivalent of weightlessness.

Korea Earth Observation Satellite Program

NASA Astrophysics Data System (ADS)

Baek, Myung-Jin; Kim, Zeen-Chul

via Korea Aerospace Research Institute (KARI) as the prime contractor in the area of Korea earth observation satellite program to enhance Korea's space program development capability. In this paper, Korea's on-going and future earth observation satellite programs are introduced: KOMPSAT- 1 (Korea Multi Purpose Satellite-1), KOMPSAT-2 and Communication, Broadcasting and Meteorological Satellite (CBMS) program. KOMPSAT-1 satellite successfully launched in December 1999 with Taurus launch vehicle. Since launch, KOMPSAT-1 is downlinking images of Korea Peninsular every day. Until now, KOMPSAT-1 has been operated more than 2 and half years without any major hardware malfunction for the mission operation. KOMPSAT-1 payload has 6.6m panchromatic spatial resolution at 685 km on-orbit and the spacecraft bus had NASA TOMS-EP (Total Ozone Mapping Spectrometer-Earth Probe) spacecraft bus heritage designed and built by TRW, U.S.A.KOMPSAT-1 program was international co-development program between KARI and TRW funded by Korean Government. be launched in 2004. Main mission objective is to provide geo-information products based on the multi-spectral high resolution sensor called Multi-Spectral Camera (MSC) which will provide 1m panchromatic and 4m multi-spectral high resolution images. ELOP of Israel is the prime contractor of the MSC payload system and KARI is the total system prime contractor including spacecraft bus development and ground segment. KARI also has the contract with Astrium of Europe for the purpose of technical consultation and hardware procurement. Based on the experience throughout KOMPSAT-1 and KOMPSAT-2 space system development, Korea is expecting to establish the infrastructure of developing satellite system. Currently, KOMPSAT-2 program is in the critical design stage. are scheduled to launch in 2008 and in 2014, respectively. The mission of CBMS consists of two areas. One is of space technology test for the communications mission, and the other is of a real

Initiation of small-satellite formations via satellite ejector

NASA Astrophysics Data System (ADS)

McMullen, Matthew G

Small satellites can be constructed at a fraction of the cost of a full-size satellite. One full-size satellite can be replaced with a multitude of small satellites, offering expanded area coverage through formation flight. However, the shortcoming to the smaller size is usually a lack of thrusting capabilities. Furthermore, current designs for small satellite deployment mechanisms are only capable of love deployment velocities (on the order of meters per second). Motivated to address this shortcoming, a conceived satellite ejector would offer a significant orbit change by ejecting the satellite at higher deployment velocities (125-200 m/s). Focusing on the applications of the ejector, it is sought to bridge the gap in prior research by offering a method to initiate formation flight. As a precursor to the initiation, the desired orbit properties to initiate the formation are specified in terms of spacing and velocity change vector. From this, a systematic method is developed to find the relationship among velocity change vector, the desired orbit's orientation, and the spacing required to initiate the formation.

Regulation of centriolar satellite integrity and its physiology.

PubMed

Hori, Akiko; Toda, Takashi

2017-01-01

Centriolar satellites comprise cytoplasmic granules that are located around the centrosome. Their molecular identification was first reported more than a quarter of a century ago. These particles are not static in the cell but instead constantly move around the centrosome. Over the last decade, significant advances in their molecular compositions and biological functions have been achieved due to comprehensive proteomics and genomics, super-resolution microscopy analyses and elegant genetic manipulations. Centriolar satellites play pivotal roles in centrosome assembly and primary cilium formation through the delivery of centriolar/centrosomal components from the cytoplasm to the centrosome. Their importance is further underscored by the fact that mutations in genes encoding satellite components and regulators lead to various human disorders such as ciliopathies. Moreover, the most recent findings highlight dynamic structural remodelling in response to internal and external cues and unexpected positive feedback control that is exerted from the centrosome for centriolar satellite integrity.

Solar power satellite life-cycle energy recovery consideration

NASA Astrophysics Data System (ADS)

Weingartner, S.; Blumenberg, J.

The construction, in-orbit installation and maintenance of a solar power satellite (SPS) will demand large amounts of energy. As a minimum requirement for an energy effective power satellite it is asked that this amount of energy be recovered. The energy effectiveness in this sense resulting in a positive net energy balance is a prerequisite for cost-effective power satellite. This paper concentrates on life-cycle energy recovery instead on monetary aspects. The trade-offs between various power generation systems (different types of solar cells, solar dynamic), various construction and installation strategies (using terrestrial or extra-terrestrial resources) and the expected/required lifetime of the SPS are reviewed. The presented work is based on a 2-year study performed at the Technical University of Munich. The study showed that the main energy which is needed to make a solar power satellite a reality is required for the production of the solar power components (up to 65%), especially for the solar cell production. Whereas transport into orbit accounts in the order of 20% and the receiving station on earth (rectenna) requires about 15% of the total energy investment. The energetic amortization time, i.e. the time the SPS has to be operational to give back the amount of energy which was needed for its production installation and operation, is about two years.

Satellite end of life constraints: Technical and organisational solutions

NASA Astrophysics Data System (ADS)

Cabrières, Bernard; Alby, Fernand; Cazaux, Christian

2012-04-01

Since 1974 with the radiocommunication satellite Symphony1, CNES launched and operated 11 GEO and 20 LEO satellites. During those 36 years, both flight segment and ground segment dramatically evolved and operational organisations and techniques equally improved. At the present time, CNES operates 1 GEO satellite and 17 LEO satellites with not much more people and costs than in 1986 when its first Satellite Operation Direction in Toulouse was only in charge of Telecom1A, Telecom1B and Spot1. This fantastic technical evolution combined with the huge increase of services to citizens and governments given by Space systems was unfortunately also associated with an enormous growth of space pollution by debris of all sizes. From the beginning, CNES was a major actor of the international effort to promote regulations in order to try to reduce or at least control this problematic situation. Internally, CNES, not only set up an operational on-call service to deal with collision risks, but decided to do its best to apply the new guidelines to the end of life of satellites under its responsibility even for those developed and launched a very long time ago. For instance, that was the case in 2009 for the reorbitation of the GEO satellite Telecom 2C (launched in 1995) and for the deorbitation of the LEO satellite Spot2 (launched in 1990). In addition, CNES prepares procedures to be able to be as exemplary as possible for its other spacecrafts whose end of life approaches. The constraints and challenges to face in order to cope with these new requirements are multiple: choice of final orbit, realistic calculation of re-entry duration, estimation of residual propellant, electric passivation, management of explosion risks… All these studies and operational experience gained will be helpful for the new role of CNES, which recently became in charge of controlling space operators in the frame of the new French space law on space operations.

A mobile communication system providing integrated voice/data services over power limited satellite channels

NASA Astrophysics Data System (ADS)

Bose, Sanjay K.; Gordon, J. J.

The modeling and analysis of a system providing integrated voice/data services to mobile terminals over a power-limited satellite channel are discussed. The mobiles use slotted Aloha random access to send requests for channel assignments to a central station. For successful requests, the actual transmission of voice/data within a call is done using the channel assigned for this purpose by the central station. The satellite channel is assumed to be power limited. Taking into account the known burstiness of voice sources (which use a voice-activated switch), the central station overassigns channels so that the average total power is below the power limit of the satellite transponder. The performance of this model is analyzed. Certain simple, static control strategies for improving performance are also proposed.

Space transportation, satellite services, and space platforms

NASA Technical Reports Server (NTRS)

Disher, J. H.

1979-01-01

The paper takes a preview of the progressive development of vehicles for space transportation, satellite services, and orbital platforms. A low-thrust upper stage of either the ion engine or chemical type will be developed to transport large spacecraft and space platforms to and from GEO. The multimission spacecraft, space telescope, and other scientific platforms will require orbital serves going beyond that provided by the Shuttle's remote manipulator system, and plans call for extravehicular activity tools, improved remote manipulators, and a remote manned work station (the cherry picker).

Aeronautical Satellite-Assisted Process for Information Exchange Through Network Technologies (Aero-SAPIENT) Conducted

NASA Technical Reports Server (NTRS)

Zernic, Michael J.

2002-01-01

Broadband satellite communications for aeronautics marries communication and network technologies to address NASA's goals in information technology base research and development, thereby serving the safety and capacity needs of the National Airspace System. This marriage of technology increases the interactivity between airborne vehicles and ground systems. It improves decision-making and efficiency, reduces operation costs, and improves the safety and capacity of the National Airspace System. To this end, a collaborative project called the Aeronautical Satellite Assisted Process for Information Exchange through Network Technologies, or Aero-SAPIENT, was conducted out of Tinker AFB, Oklahoma, during November and December 2000.

Electrostatic protection of the solar power satellite and rectenna. Part 1: Protection of the solar power satellite

NASA Technical Reports Server (NTRS)

1980-01-01

Several features of the interactions of the Solar Power Satellite (SPS) with its space environment are examined theoretically. The voltages produced at various surfaces due to space plasmas and the plasma leakage currents through the kapton and sapphire solar cell blankets are calculated. At geosynchronous orbit, this parasitic power loss is only 0.7%, and is easily compensated by oversizing. At low Earth orbit, the power loss is potentially much larger (3%), and anomalous arcing is expected for the EOTV high voltage negative surfaces. Preliminary results of a three dimensional self consistent plasma and electric field computer program are presented, confirming the validity of the predictions made from the one dimensional models. Lastly, magnetic shielding of the satellite is considered to reduce the power drain and to protect the solar cells from energetic electron and plasma ion bombardment. It is concluded that minor modifications can allow the SPS to operate safely and efficiently in its space environment. Subsequent design changes will substantially alter the basic conclusions.

DNA methylation analysis on satellite I region in blastocysts obtained from somatic cell cloned cattle.

PubMed

Yamanaka, Ken-Ichi; Kaneda, Masahiro; Inaba, Yasushi; Saito, Koji; Kubota, Kaiyu; Sakatani, Miki; Sugimura, Satoshi; Imai, Kei; Watanabe, Shinya; Takahashi, Masashi

2011-08-01

Many observations have been made on cloned embryos and on adult clones by somatic cell nuclear transfer (SCNT), but it is still unclear whether the progeny of cloned animals is presenting normal epigenetic status. Here, in order to accumulate the information for evaluating the normality of cloned cattle, we analyzed the DNA methylation status on satellite I region in blastocysts obtained from cloned cattle. Embryos were produced by artificial insemination (AI) to non-cloned or cloned dams using semen from non-cloned or cloned sires. After 7 days of AI, embryos at blastocyst stage were collected by uterine flushing. The DNA methylation levels in embryos obtained by using semen and/or oocytes from cloned cattle were similar to those in in vivo embryos from non-cloned cattle. In contrast, the DNA methylation levels in SCNT embryos were significantly higher (P < 0.01) than those in in vivo embryos from non-cloned and cloned cattle, approximately similar to those in somatic cells used as donor cells. Thus, this study provides useful information that epigenetic status may be normal in the progeny of cloned cattle, suggesting the normality of germline cells in cloned cattle. 2011 The Authors. Animal Science Journal © 2011 Japanese Society of Animal Science.

The Next Landsat Satellite: The Landsat Data Continuity Mission

NASA Technical Reports Server (NTRS)

Rons, James R.; Dwyer, John L.; Barsi, Julia A.

2012-01-01

The Landsat program is one of the longest running satellite programs for Earth observations from space. The program was initiated by the launch of Landsat 1 in 1972. Since then a series of six more Landsat satellites were launched and at least one of those satellites has been in operations at all times to continuously collect images of the global land surface. The Department of Interior (DOI) U.S. Geological Survey (USGS) preserves data collected by all of the Landsat satellites at their Earth Resources Observation and Science (EROS) Center in Sioux Falls, South Dakota. This 40-year data archive provides an unmatched record of the Earth's land surface that has undergone dramatic changes in recent decades due to the increasing pressure of a growing population and advancing technologies. EROS provides the ability for anyone to search the archive and order digital Landsat images over the internet for free. The Landsat data are a public resource for observing, characterizing, monitoring, trending, and predicting land use change over time providing an invaluable tool for those addressing the profound consequences of those changes to society. The most recent launch of a Landsat satellite occurred in 1999 when Landsat 7 was placed in orbit. While Landsat 7 remains in operation, the National Aeronautics and Space Administration (NASA) and the DOI/ USGS are building its successor satellite system currently called the Landsat Data Continuity Mission (LDCM). NASA has the lead for building and launching the satellite that will carry two Earth-viewing instruments, the Operational Land Imager (OLI) and the Thermal Infrared Sensor (TIRS). The OLI will take images that measure the amount of sunlight reflected by the land surface at nine wavelengths of light with three of those wavelengths beyond the range of human vision. T1RS will collect coincident images that measure light emitted by the land surface as a function of surface temperature at two longer wavelengths well beyond the

Educational Pico-Satellite Project CUBESAT - University of Tokyo's CUBESAT XI and its Operation Plan

NASA Astrophysics Data System (ADS)

Tsuda, Y.; Sako, N.; Eishima, T.; Ito, T.; Arikawa, Y.; Miyamura, N.

2002-01-01

University of Tokyo ISSL (Intelligent Space Systems Laboratory) has been developing a pico-satellite called "CubeSat" as an international joint program. In CubeSat project, 10cm cubic satellites have been developed by several universities and launched to the low-earth orbit altogether by Russian rocket "Dnepr". ISSL has developed "XI" series ([sai]: X-factor Investigator) satellites, and the flight model is already fabricated and ready for delivery. The mission of XI satellite is the on-orbit technology demonstration of the ultra-small satellite bus system with an extensive use of commercial-off-the-shelf components. XI transmits the Morse beacon and FM packet telemetry which provides the health data of the satellite. Additionally, XI has a CMOS camera which provides 15,000 pixels panchromatic images as an advanced mission. Ground operation is one of the key issues for CubeSats. Now we are promoting international ground station network in which several universities' ground stations connected by internet collaboratively operate university-built small satellites, which enlarges the link opportunity. Collaboration with amateur HAM engineers is also indispensable for search for the satellite or get beacon signal to estimate the satellite orbit. We are now developing operation concept based on these ideas. As the launch is scheduled in this fall, the operation plan will be fixed at the time of this conference. In this presentation the final design of ISSL's CubeSat XI and operation plan will be presented.

A high-fidelity N-body ephemeris generator for satellites in Earth orbit

NASA Astrophysics Data System (ADS)

Simmons, David R.

1991-10-01

A program is currently used for mission planning called the Analytic Satellite Ephemeris Program (ASEP), which produces projected data for orbits that remain fairly close to Earth. Lunar and solar perturbations are taken into account in another program called GRAVE. This project is a revision of GRAVE which incorporates more flexible means of input for initial data, provides additional kinds of output information, and makes use of structured programming techniques to make the program more understandable and reliable. The computer program ORBIT was tested against tracking data for the first 313 days of operation of the CRRES satellite. A sample graph is given comparing the semi-major axis calculated by the program with the values supplied by NORAD. When calculated for points at which CRRES passes through the ascending node, the argument of perigee, the right ascension of the ascending node, and the mean anomaly all stay within about a degree of the corresponding values from NORAD; the inclination of the orbital plane is much closer. The program value of the eccentricity is in error by no more than 0.0002.

Swarm satellite mission scheduling & planning using Hybrid Dynamic Mutation Genetic Algorithm