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Sample records for centrosome-associated nucleocytoplasmic shuttling

  1. Cyclin G2 is a centrosome-associated nucleocytoplasmic shuttling protein that influences microtubule stability and induces a p53-dependent cell cycle arrest

    SciTech Connect

    Arachchige Don, Aruni S.; Dallapiazza, Robert F.; Bennin, David A.; Brake, Tiffany; Cowan, Colleen E.; Horne, Mary C. . E-mail: mary-horne@uiowa.edu

    2006-12-10

    , the mature centriole present at microtubule foci, indicates that cyclin G2 resides primarily on the mother centriole. Copurification of cyclin G2 and PP2A subunits with microtubules and centrosomes, together with the effects of ectopic cyclin G2 on cell cycle progression, nuclear morphology and microtubule growth and stability, suggests that cyclin G2 may modulate the cell cycle and cellular division processes through modulation of PP2A and centrosomal associated activities.

  2. Nucleocytoplasmic shuttling of galectin-3.

    PubMed

    Arnoys, Eric J; Ackerman, Cheri M; Wang, John L

    2015-01-01

    A large number of observations on the nuclear versus cytoplasmic distribution of galectin-3 have been reported, correlating the presence or absence of the protein in a particular compartment of the cell to various parameters such as source of the cells under study, specific cell type, culture conditions, proliferation status of the cell/culture, or neoplastic transformation. In fact, galectin-3 exhibits the phenomenon of nucleocytoplasmic shuttling, defined as the repeated bidirectional movement of a protein across the nuclear pore complex. Nevertheless, the finding that galectin-3 can show a predominantly nuclear localization under one set of conditions and a prominent cytoplasmic localization under other conditions suggests specific and regulated mechanisms of balance between cytoplasmic anchorage, nuclear import, nuclear retention, and nuclear export. One key consideration in the understanding of these processes is the definition of the signals and receptors that mediate the transport. In this chapter, we describe the experimental procedures that have allowed us to document the phenomenon of nucleocytoplasmic shuttling and the identification of the nuclear localization signal as well as the nuclear export signal. PMID:25253159

  3. Nucleocytoplasmic Shuttling of Endocytic Proteins

    PubMed Central

    Vecchi, Manuela; Polo, Simona; Poupon, Viviane; van de Loo, Jan-Willem; Benmerah, Alexandre; Di Fiore, Pier Paolo

    2001-01-01

    Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and α-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic shuttling of endocytic proteins are apparently independent processes, since inhibition of endocytosis did not appreciably alter nuclear translocation of endocytic proteins, and blockade of nuclear export did not change the initial rate of endocytosis. In the nucleus, eps15 and CALM acted as positive modulators of transcription in a GAL4-based transactivation assay, thus raising the intriguing possibility that some endocytic proteins play a direct or indirect role in transcriptional regulation. PMID:11425879

  4. Nucleocytoplasmic shuttling of the progesterone receptor.

    PubMed Central

    Guiochon-Mantel, A; Lescop, P; Christin-Maitre, S; Loosfelt, H; Perrot-Applanat, M; Milgrom, E

    1991-01-01

    The nuclear localization of the progesterone receptor is mediated by two signal sequences: one is constitutive and lies in the hinge region (between the DNA and steroid binding domains), the other is hormone dependent and is localized in the second zinc finger of the DNA binding domain. The use of various inhibitors of energy synthesis in cells expressing permanently or transiently the wild-type receptor or a receptor mutated within the nuclear localization signals, demonstrated that the nuclear residency of the receptor reflects a dynamic situation: the receptor diffusing into the cytoplasm and being constantly and actively transported back into the nucleus. The existence of this nucleo-cytoplasmic shuttle mechanism was confirmed by receptor transfer from one nucleus to the other in heterokaryons. Preliminary evidence was obtained, using oestrogen receptor, that this phenomenon may be of general significance for steroid receptors. Images PMID:1935904

  5. Nucleocytoplasmic Shuttling Activity of Ataxin-3

    PubMed Central

    Maciel, Patrícia; Carvalho, Ana Luísa

    2009-01-01

    Spinocerebellar ataxia type-3, also known as Machado-Joseph Disease (MJD), is one of many inherited neurodegenerative disorders caused by polyglutamine-encoding CAG repeat expansions in otherwise unrelated genes. Disease protein misfolding and aggregation, often within the nucleus of affected neurons, characterize polyglutamine disorders. Several evidences have implicated the nucleus as the primary site of pathogenesis for MJD. However, the molecular determinants for the nucleocytoplasmic transport of human ataxin-3 (Atx3), the protein which is mutated in patients with MJD, are not characterized. In order to characterize the nuclear shuttling activity of Atx3, we performed yeast nuclear import assays and found that Atx3 is actively imported into the nucleus, by means of a classical nuclear localizing sequence formed by a cluster of lysine and arginine residues. On the other hand, when active nuclear export was inhibited using leptomycin B, a specific inhibitor of the nuclear export receptor CRM1, both endogenous Atx3 and transfected GFP-Atx3 accumulated inside the nucleus of a subpopulation of COS-7 cells, whereas both proteins are normally predominant in the cytoplasm. Additionally, using a Rev(1.4)-GFP nuclear export assay, we performed an extensive analysis of six putative aliphatic nuclear export motifs identified in Atx3 amino acid sequence. Although none of the tested peptide sequences were found to drive nuclear export when isolated, we have successfully mapped the region of Atx3 responsible for its CRM1-independent nuclear export activity. Curiously, the N-terminal Josephin domain alone is exported into the cytoplasm, but the nuclear export activity of Atx3 is significantly enhanced in a longer construct that is truncated after the two ubiquitin interaction motifs, upstream from the polyQ tract. Our data show that Atx3 is actively imported to and exported from the cell nucleus, and that its nuclear export activity is dependent on a motif located at its N

  6. Molecular Pathways: Anticancer Activity by Inhibition of Nucleocytoplasmic Shuttling.

    PubMed

    Conforti, Fabio; Wang, Yisong; Rodriguez, Jose A; Alberobello, Anna Teresa; Zhang, Yu-Wen; Giaccone, Giuseppe

    2015-10-15

    A dynamic distribution between nucleus and cytoplasm (nucleocytoplasmic shuttling) is one of the control mechanisms adapted by normal cells to regulate the activity of a variety of molecules. Growing evidence suggests that dysregulation of the nucleocytoplasmic shuttling is involved in promoting abnormal cell survival, tumor progression, and drug resistance, and is associated with poor cancer prognosis. Aberrant nucleocytoplasmic shuttling in cancer cells may result from a hyperactive status of diverse signal-transduction pathways, such as the PI3K-AKT and MAPK pathways, or from alterations in the general nuclear import/export machinery. Among the large number of molecules involved in the shuttling process, exportin XPO1, also known as chromosome region maintenance 1, appears to play a particularly prominent role in pathogenesis of both hematological malignancies and solid tumors. Given the importance of nucleocytoplasmic shuttling in cancer pathogenesis and the rapidly expanding knowledge in this field, attempts have been made to develop compounds able to revert the aberrant nucleocytoplasmic shuttling. A promising new drug, KPT-330 (Selinexor), which belongs to the class of XPO1 inhibitors called selective inhibitors of nuclear export, is now being tested in phase I/II clinical trials. PMID:26324742

  7. Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes

    PubMed Central

    Inoue, Alexandre Haruo; Serpeloni, Mariana; Hiraiwa, Priscila Mazzocchi; Yamada-Ogatta, Sueli Fumie; Muniz, João Renato Carvalho; Motta, Maria Cristina Machado; Vidal, Newton Medeiros; Goldenberg, Samuel; Ávila, Andréa Rodrigues

    2014-01-01

    Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor. PMID:25313564

  8. Characterization of molecular determinants for nucleocytoplasmic shuttling of PRV UL54

    SciTech Connect

    Li Meili; Wang Shuai; Cai Mingsheng; Guo Hong; Zheng Chunfu

    2011-09-01

    The pseudorabies virus (PRV) early protein UL54 is a homologue of the herpes simplex virus 1 (HSV-1) immediate-early protein ICP27, which is a multifunctional protein and essential for HSV-1 infection. To determine if UL54 might shuttle between the nucleus and cytoplasm, as has been shown for its homologues in human herpesviruses, the molecular determinants for its nucleocytoplasmic shuttling were investigated. Heterokaryon assays demonstrated that UL54 was a nucleocytoplasmic shuttling protein and this property could not be blocked by leptomycin B, an inhibitor of chromosome region maintenance 1 (CRM1). However, TAP/NXF1 promoted the nuclear export of UL54 and interacted with UL54, suggesting that UL54 shuttles between the nucleus and the cytoplasm via a TAP/NXF1, but not CRM1, dependent nuclear export pathway. Furthermore, UL54 was demonstrated to target to the nucleus through a classic Ran-, importin {beta}1- and {alpha}5-dependent nuclear import mechanism.

  9. ERK1 nucleocytoplasmic shuttling rate depends on specific N-terminal aminoacids

    SciTech Connect

    Marchi, Matilde; NEST-INFM, Scuola Normale Superiore, Pisa ; Pancrazi, Laura; Maffei, Margherita; Institute of Food Science CNR, Avellino ; Ratto, G. Michele; Costa, Mario

    2010-07-23

    Despite ERK1 and ERK2 were considered interchangeable isoforms for a long time, their roles are now emerging as only partially overlapping. We recently reported that the nucleocytoplasmic trafficking of GFP-tagged ERK1 is slower than that of ERK2, this difference being caused by a unique domain of ERK1 located at its N-terminus (ERK1-Nt). In the present report we further investigated this issue by asking which were the specific aminoacids involved in such process. By photobleaching strategy, we demonstrated that ERK1-Nt is a domain capable to slow down the nucleocytoplasmic shuttling rate even of a small cargo protein. ERK1-Nt was then dissected into three regions as follows: 1 (aa 1-9), 2 (aa 10-29) and 3, (aa 30-39) that were deleted or mutated at specific sites. Dynamic imaging assessment of the role played by each region in determining the shuttling rate revealed that: region 1 has no significant role, region 2 and specific aminoacids of region 3 (V{sub 31}, K{sub 33,} P{sub 36}) are critical, but singularly do not totally account for the difference in the shuttling rate between ERK1 and 2. Finally, we demonstrated that the nucleocytoplasmic shuttling rate of a passively diffusing protein (mRED) is inversely related to ERK1-Nt-GFP concentrations inside the cell, thus suggesting that ERK1-Nt-GFP occupies the nuclear pore perhaps because of an important affinity of ERK1-Nt for nucleoporins. In conclusion, ERK1-Nt is a domain able per se to confer a slower shuttling rate to a cargo protein. Specific regions within this domain were identified as responsible for this biophysical property.

  10. Nucleocytoplasmic shuttling: a novel in vivo property of antisense phosphorothioate oligodeoxynucleotides

    PubMed Central

    Lorenz, Peter; Misteli, Tom; Baker, Brenda F.; Bennett, C. Frank; Spector, David L.

    2000-01-01

    Phosphorothioate oligodeoxynucleotides (P=S ODNs) are frequently used as antisense agents to specifically interfere with the expression of cellular target genes. However, the cell biological properties of P=S ODNs are poorly understood. Here we show that P=S ODNs were able to continuously shuttle between the nucleus and the cytoplasm and that shuttling P=S ODNs retained their ability to act as antisense agents. The shuttling process shares characteristics with active transport since it was inhibited by chilling and ATP depletion in vivo. Transport was carrier-mediated as it was saturable, and nuclear pore complex-mediated as it was sensitive to treatment with wheatgerm agglutinin. Oligonucleotides without a P=S backbone chemistry were only weakly restricted in their migration by chilling, ATP depletion and wheatgerm agglutinin and thus moved by diffusion. P=S ODN shuttling was only moderately affected by disruption of the Ran/RCC1 system. We propose that P=S ODNs shuttle through their binding to yet unidentified cellular molecules that undergo nucleocytoplasmic transport via a pathway that is not as strongly dependent on the Ran/RCC1 system as nuclear export signal-mediated protein export, U-snRNA, tRNA and mRNA export. The shuttling property of P=S ODNs must be taken into account when considering the mode and site of action of these antisense agents. PMID:10606658

  11. Space shuttling in the cell: nucleocytoplasmic transport and microtubule organization during the cell cycle.

    PubMed

    Sato, Masamitsu; Toda, Takashi

    2010-01-01

    Microtubules form a multifunctional filamentous structure essential for the cell. In interphase, microtubules form networks in the cytoplasm and play pivotal roles in cell polarity and intracellular transport of various biomolecules. In mitosis, microtubules dramatically change their morphology to assemble the mitotic spindle, thereby pulling the chromosomes toward the spindle poles. One long-standing question is how microtubules are reorganized upon mitotic entry. Yeast cells undergo closed mitosis, in which the nuclear envelope persists, whereas higher eukaryotes undergo open mitosis, in which the nuclear envelope breaks down. Microtubule reorganization must be controlled by selective localization of microtubule-assembly factors. Recent findings in fission yeast indicate that several microtubule-associated proteins (MAPs) shuttle between the cytoplasm and the nucleus through regulation by Ran GTPase, the universal organizer of nucleocytoplasmic transport. Furthermore, the synergistic interplay of Ran and cyclin-dependent kinase (CDK) induces the critical spatiotemporal shift of modes in microtubule assembly from cytoplasmic arrays to nuclear spindles. A MAP complex Alp7/TACC-Alp14/TOG undergoes nucleocytoplasmic shuttling in interphase, whereas it is retained in the mitotic nucleus through a decrease of its nuclear export by CDK. Our understanding of how microtubules are reorganized during the cell cycle is beginning to emerge. PMID:21327068

  12. hMSH5 is a nucleocytoplasmic shuttling protein whose stability depends on its subcellular localization

    PubMed Central

    Lahaye, François; Lespinasse, Françoise; Staccini, Pascal; Palin, Lucile; Paquis-Flucklinger, Véronique; Santucci-Darmanin, Sabine

    2010-01-01

    MSH5 is a MutS-homologous protein required for meiotic DNA recombination. In addition, recent studies suggest that the human MSH5 protein (hMSH5) participates to mitotic recombination and to the cellular response to DNA damage and thus raise the possibility that a tight control of hMSH5 function(s) may be important for genomic stability. With the aim to characterize mechanisms potentially involved in the regulation of hMSH5 activity, we investigated its intracellular trafficking properties. We demonstrate that hMSH5 possesses a CRM1-dependent nuclear export signal (NES) and a nuclear localization signal that participates to its nuclear targeting. Localization analysis of various mutated forms of hMSH5 by confocal microscopy indicates that hMSH5 shuttles between the nucleus and the cytoplasm. We also provide evidence suggesting that hMSH5 stability depends on its subcellular compartmentalization, hMSH5 being much less stable in the nucleus than in the cytoplasm. Together, these data suggest that hMSH5 activity may be regulated by nucleocytoplasmic shuttling and nuclear proteasomal degradation, both of these mechanisms contributing to the control of nuclear hMSH5 content. Moreover, data herein also support that in tissues where both hMSH5 and hMSH4 proteins are expressed, hMSH5 might be retained in the nucleus through masking of its NES by binding of hMSH4. PMID:20185565

  13. BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon heat stress

    SciTech Connect

    Jin, Young-Hee; Ahn, Sang-Gun; Kim, Soo-A.

    2015-08-21

    Bcl2-associated athoanogene (BAG) 3 is a member of the co-chaperone BAG family. It is induced by stressful stimuli such as heat shock and heavy metals, and it regulates cellular adaptive responses against stressful conditions. In this study, we identified a novel role for BAG3 in regulating the nuclear shuttling of HSF1 during heat stress. The expression level of BAG3 was induced by heat stress in HeLa cells. Interestingly, BAG3 rapidly translocalized to the nucleus upon heat stress. Immunoprecipitation assay showed that BAG3 interacts with HSF1 under normal and stressed conditions and co-translocalizes to the nucleus upon heat stress. We also demonstrated that BAG3 interacts with HSF1 via its BAG domain. Over-expression of BAG3 down-regulates the level of nuclear HSF1 by exporting it to the cytoplasm during the recovery period. Depletion of BAG3 using siRNA results in reduced nuclear HSF1 and decreased Hsp70 promoter activity. BAG3 in MEF(hsf1{sup −/−}) cells actively translocalizes to the nucleus upon heat stress suggesting that BAG3 plays a key role in the processing of the nucleocytoplasmic shuttling of HSF1 upon heat stress. - Highlights: • The expression level of BAG3 is induced by heat stress. • BAG3 translocates to the nucleus upon heat stress. • BAG3 interacts with HSF1 and co-localizes to the nucleus. • BAG3 is a key regulator for HSF1 nuclear shuttling.

  14. GNL3L Is a Nucleo-Cytoplasmic Shuttling Protein: Role in Cell Cycle Regulation

    PubMed Central

    Thoompumkal, Indu Jose; Mahalingam, Sundarasamy

    2015-01-01

    GNL3L is an evolutionarily conserved high molecular weight GTP binding nucleolar protein belonging to HSR1-MMR1 subfamily of GTPases. The present investigation reveals that GNL3L is a nucleo-cytoplasmic shuttling protein and its export from the nucleus is sensitive to Leptomycin B. Deletion mutagenesis reveals that the C-terminal domain (amino acids 501–582) is necessary and sufficient for the export of GNL3L from the nucleus and the exchange of hydrophobic residues (M567, L570 and 572) within the C-terminal domain impairs this process. Results from the protein-protein interaction analysis indicate that GNL3L interaction with CRM1 is critical for its export from the nucleus. Ectopic expression of GNL3L leads to lesser accumulation of cells in the ‘G2/M’ phase of cell cycle whereas depletion of endogenous GNL3L results in ‘G2/M’ arrest. Interestingly, cell cycle analysis followed by BrdU labeling assay indicates that significantly increased DNA synthesis occurs in cells expressing nuclear export defective mutant (GNL3L∆NES) compared to the wild type or nuclear import defective GNL3L. Furthermore, increased hyperphosphorylation of Rb at Serine 780 and the upregulation of E2F1, cyclins A2 and E1 upon ectopic expression of GNL3L∆NES results in faster ‘S’ phase progression. Collectively, the present study provides evidence that GNL3L is exported from the nucleus in CRM1 dependent manner and the nuclear localization of GNL3L is important to promote ‘S’ phase progression during cell proliferation. PMID:26274615

  15. Subcellular Localization of Class II HDAs in Arabidopsis thaliana: Nucleocytoplasmic Shuttling of HDA15 Is Driven by Light

    PubMed Central

    Alinsug, Malona V.; Chen, Fang Fang; Luo, Ming; Tai, Ready; Jiang, Liwen; Wu, Keqiang

    2012-01-01

    Class II histone deacetylases in humans and other model organisms undergo nucleocytoplasmic shuttling. This unique functional regulatory mechanism has been well elucidated in eukaryotic organisms except in plant systems. In this study, we have paved the baseline evidence for the cytoplasmic and nuclear localization of Class II HDAs as well as their mRNA expression patterns. RT-PCR analysis on the different vegetative parts and developmental stages reveal that Class II HDAs are ubiquitously expressed in all tissues with minimal developmental specificity. Moreover, stable and transient expression assays using HDA-YFP/GFP fusion constructs indicate cytoplasmic localization of HDA5, HDA8, and HDA14 further suggesting their potential for nuclear transport and deacetylating organellar and cytoplasmic proteins. Organelle markers and stains confirm HDA14 to abound in the mitochondria and chloroplasts while HDA5 localizes in the ER. HDA15, on the other hand, shuttles in and out of the nucleus upon light exposure. In the absence of light, it is exported out of the nucleus where further re-exposition to light treatments signals its nuclear import. Unlike HDA5 which binds with 14-3-3 proteins, HDA15 fails to interact with these chaperones. Instead, HDA15 relies on its own nuclear localization and export signals to navigate its subcellular compartmentalization classifying it as a Class IIb HDA. Our study indicates that nucleocytoplasmic shuttling is indeed a hallmark for all eukaryotic Class II histone deacetylases. PMID:22363501

  16. The Q-rich/PST domain of the AHR regulates both ligand-induced nuclear transport and nucleocytoplasmic shuttling

    PubMed Central

    Tkachenko, Anna; Henkler, Frank; Brinkmann, Joep; Sowada, Juliane; Genkinger, Doris; Kern, Christian; Tralau, Tewes; Luch, Andreas

    2016-01-01

    The aryl hydrocarbon receptor (AHR) shuttles continuously between cytoplasm and nucleus, unless ligand-binding triggers association with the AHR nuclear translocator (ARNT) and subsequent binding to cognate DNA motifs. We have now identified Val 647 as mandatory residue for export from the nucleus and AHR-function. This residue prevents inactivation of the receptor as a consequence of nuclear sequestration via constitutive import. Concomitantly mutants lacking this residue are exclusively localised in the nucleus. Although ligands accelerate nuclear import transiently, stable nuclear transition depends on a motif adjacent to Val 647 that comprises residues 650–661. Together, this defined region within the Q-rich domain regulates intracellular trafficking of the AHR in context of both nucleocytoplasmic shuttling and receptor activation. Nuclear export therefore depends on the previously characterised N-terminal NES and the newly identified motif that includes V647. Nucleocytoplasmic distribution of full-length human AHR is further affected by a section of the PST domain that shows sequence similarities with nuclear export signals. In concert, these motifs maintain a predominant cytoplasmic compartmentalisation, receptive for ligand binding. PMID:27535013

  17. The Q-rich/PST domain of the AHR regulates both ligand-induced nuclear transport and nucleocytoplasmic shuttling.

    PubMed

    Tkachenko, Anna; Henkler, Frank; Brinkmann, Joep; Sowada, Juliane; Genkinger, Doris; Kern, Christian; Tralau, Tewes; Luch, Andreas

    2016-01-01

    The aryl hydrocarbon receptor (AHR) shuttles continuously between cytoplasm and nucleus, unless ligand-binding triggers association with the AHR nuclear translocator (ARNT) and subsequent binding to cognate DNA motifs. We have now identified Val 647 as mandatory residue for export from the nucleus and AHR-function. This residue prevents inactivation of the receptor as a consequence of nuclear sequestration via constitutive import. Concomitantly mutants lacking this residue are exclusively localised in the nucleus. Although ligands accelerate nuclear import transiently, stable nuclear transition depends on a motif adjacent to Val 647 that comprises residues 650-661. Together, this defined region within the Q-rich domain regulates intracellular trafficking of the AHR in context of both nucleocytoplasmic shuttling and receptor activation. Nuclear export therefore depends on the previously characterised N-terminal NES and the newly identified motif that includes V647. Nucleocytoplasmic distribution of full-length human AHR is further affected by a section of the PST domain that shows sequence similarities with nuclear export signals. In concert, these motifs maintain a predominant cytoplasmic compartmentalisation, receptive for ligand binding. PMID:27535013

  18. The BRO proteins of Bombyx mori nucleopolyhedrovirus are nucleocytoplasmic shuttling proteins that utilize the CRM1-mediated nuclear export pathway

    SciTech Connect

    Kang, Won Kyung . E-mail: wkkang@riken.jp; Kurihara, Masaaki . E-mail: mkuri@riken.jp; Matsumoto, Shogo . E-mail: smatsu@riken.jp

    2006-06-20

    The BRO proteins of Bombyx mori nucleopolyhedrovirus (BmNPV) display a biphasic pattern of intracellular localization during infection. At early times, they reside in the nucleus but then show both cytoplasmic and nuclear localization as the infection proceeds. Therefore, we examined the possibility of nuclear export. Using inhibitors, we reveal that BmNPV BRO proteins shuttle between the nucleus and cytoplasm. Mutations on the leucine-rich region of BRO proteins resulted in nuclear accumulation of transiently expressed proteins, suggesting that this region functions as a CRM1-dependent nuclear export signal (NES). On the contrary, mutant BRO-D with an altered NES did not show nuclear accumulation in infected cells, although protein production seemed to be reduced. RT-PCR analysis showed that the lower level of protein production was due to a reduction in RNA synthesis. Taken together, our results suggest that BRO proteins are nucleocytoplasmic shuttling proteins that utilize the CRM1-mediated nuclear export pathway.

  19. p35 Regulates the CRM1-Dependent Nucleocytoplasmic Shuttling of Nuclear Hormone Receptor Coregulator-Interacting Factor 1 (NIF-1)

    PubMed Central

    Zhao, Xiao-Su; Fu, Wing-Yu; Chien, Winnie W. Y.; Li, Zhen; Fu, Amy K. Y.; Ip, Nancy Y.

    2014-01-01

    Cyclin-dependent kinase 5 (Cdk5) is a proline-directed serine/threonine kinase, which plays critical roles in a wide spectrum of neuronal functions including neuronal survival, neurite outgrowth, and synapse development and plasticity. Cdk5 activity is controlled by its specific activators: p35 or p39. While knockout studies reveal that Cdk5/p35 is critical for neuronal migration during early brain development, functions of Cdk5/p35 have been unraveled through the identification of the interacting proteins of p35, most of which are Cdk5/p35 substrates. However, it remains unclear whether p35 can regulate neuronal functions independent of Cdk5 activity. Here, we report that a nuclear protein, nuclear hormone receptor coregulator (NRC)-interacting factor 1 (NIF-1), is a new interacting partner of p35. Interestingly, p35 regulates the functions of NIF-1 independent of Cdk5 activity. NIF-1 was initially discovered as a transcriptional regulator that enhances the transcriptional activity of nuclear hormone receptors. Our results show that p35 interacts with NIF-1 and regulates its nucleocytoplasmic trafficking via the nuclear export pathway. Furthermore, we identified a nuclear export signal on p35; mutation of this site or blockade of the CRM1/exportin-dependent nuclear export pathway resulted in the nuclear accumulation of p35. Intriguingly, blocking the nuclear export of p35 attenuated the nuclear accumulation of NIF-1. These findings reveal a new p35-dependent mechanism in transcriptional regulation that involves the nucleocytoplasmic shuttling of transcription regulators. PMID:25329792

  20. The nucleocytoplasmic shuttling protein CIZ reduces adult bone mass by inhibiting bone morphogenetic protein-induced bone formation.

    PubMed

    Morinobu, Mikihiko; Nakamoto, Tetsuya; Hino, Kazunori; Tsuji, Kunikazu; Shen, Zhong-Jian; Nakashima, Kazuhisa; Nifuji, Akira; Yamamoto, Haruyasu; Hirai, Hisamaru; Noda, Masaki

    2005-03-21

    Osteoporosis is a major health problem; however, the mechanisms regulating adult bone mass are poorly understood. Cas-interacting zinc finger protein (CIZ) is a nucleocytoplasmic shuttling protein that localizes at cell adhesion plaques that form where osteoblasts attach to substrate. To investigate the potential role of CIZ in regulating adult bone mass, we examined the bones in CIZ-deficient mice. Bone volume was increased and the rates of bone formation were increased in CIZ-deficient mice, whereas bone resorption was not altered. CIZ deficiency enhanced the levels of mRNA expression of genes encoding proteins related to osteoblastic phenotypes, such as alkaline phosphatase (ALP) as well as osterix mRNA expression in whole long bones. Bone marrow cells obtained from the femora of CIZ-deficient mice revealed higher ALP activity in culture and formed more mineralized nodules than wild-type cells. CIZ deficiency enhanced bone morphogenetic protein (BMP)-induced osteoblastic differentiation in bone marrow cells in cultures, indicating that BMP is the target of CIZ action. CIZ deficiency increased newly formed bone mass after femoral bone marrow ablation in vivo. Finally, BMP-2-induced bone formation on adult mouse calvariae in vivo was enhanced by CIZ deficiency. These results establish that CIZ suppresses the levels of adult bone mass through inhibition of BMP-induced activation of osteoblasts. PMID:15781586

  1. Nucleocytoplasmic shuttling of tRNAs and implication of the cytosolic Hsp70 system in tRNA import.

    PubMed

    Yoshihisa, Tohru

    2015-01-01

    tRNAs, a class of non-coding RNAs essential for translation, are unique among cytosolic RNA species in that they shuttle between the nucleus and cytoplasm during their life. Although their export from the nucleus has been studied in detail, limited information on import machinery was available. Our group recently reported that Ssa2p, one of major cytosolic Hsp70s in Saccharomyces cerevisiae, acts as a crucial factor for tRNA import upon nutrient starvation. Ssa2p can bind tRNAs and a nucleoporin directly in an ATP-sensitive manner, suggesting that it acts as a nuclear import carrier for tRNAs, like importin-β proteins. In vitro assays revealed that Ssa2p binds tRNA specifically but has preference for loosely folded tRNAs. In this Extra View, these features of Ssa2p as a new import factor is discussed with other recent findings related to nucleocytoplasmic transport of tRNAs reported from other groups. PMID:26280499

  2. Nucleocytoplasmic shuttling of tRNAs and implication of the cytosolic Hsp70 system in tRNA import

    PubMed Central

    Yoshihisa, Tohru

    2015-01-01

    tRNAs, a class of non-coding RNAs essential for translation, are unique among cytosolic RNA species in that they shuttle between the nucleus and cytoplasm during their life. Although their export from the nucleus has been studied in detail, limited information on import machinery was available. Our group recently reported that Ssa2p, one of major cytosolic Hsp70s in Saccharomyces cerevisiae, acts as a crucial factor for tRNA import upon nutrient starvation. Ssa2p can bind tRNAs and a nucleoporin directly in an ATP-sensitive manner, suggesting that it acts as a nuclear import carrier for tRNAs, like importin-β proteins. In vitro assays revealed that Ssa2p binds tRNA specifically but has preference for loosely folded tRNAs. In this Extra View, these features of Ssa2p as a new import factor is discussed with other recent findings related to nucleocytoplasmic transport of tRNAs reported from other groups. PMID:26280499

  3. Characterization of nucleocytoplasmic shuttling and intracellular localization signals in Duck Enteritis Virus UL54.

    PubMed

    Liu, Chaoyue; Cheng, Anchun; Wang, Mingshu; Chen, Shun; Jia, Renyong; Zhu, Dekang; Liu, Mafeng; Sun, Kunfeng; Yang, Qiao; Chen, Xiaoyue

    2016-08-01

    Duck Enteritis virus (DEV) UL54 is a homolog of herpes simplex virus-1 (HSV-1) trafficking protein ICP27, which plays an essential role in infection. In this study, DEV UL54 shuttling between the nucleus and cytoplasm was verified with a heterokaryon assay. One predicted nuclear export sequence (NES) (339-348 aa) was shown to be functional and chromosomal region maintenance 1 (CRM1)-dependent; however, the insensitivity of UL54 to Leptomycin B (LMB) and NES mutation suggests that other mechanisms are responsible for the observed nuclear export. Next, three non-classical nuclear localization sequences (NLSs), referred to as NLS1 (105-122 aa), NLS2 (169-192 aa) and NLS3 (257-274 aa), were identified. Furthermore, a recombinant DEV with the UL54 NLSs deleted (DEV- UL54 mNLSs) was constructed and showed that UL54 NLSs moderately affected DEV growth. PMID:27157269

  4. Analysing the impact of nucleo-cytoplasmic shuttling of β-catenin and its antagonists APC, Axin and GSK3 on Wnt/β-catenin signalling.

    PubMed

    Schmitz, Yvonne; Rateitschak, Katja; Wolkenhauer, Olaf

    2013-11-01

    The canonical Wnt signalling pathway plays a critical role in development and disease. The key player of the pathway is β-catenin. Its activity is mainly regulated by the destruction complex consisting of APC, Axin and GSK3. In the nucleus, the complex formation of β-catenin and TCF initiates target gene expression. Our study provides a comprehensive analysis of the role of nucleo-cytoplasmic shuttling of APC, Axin, and GSK3 and the inactivation of β-catenin by the destruction complex in Wnt/β-catenin signalling. We address the following questions: Can nucleo-cytoplasmic shuttling of APC, Axin and GSK3 increase the [β-catenin/TCF] concentration? And, how is the [β-catenin/TCF] concentration influenced by phosphorylation and subsequent degradation of nuclear β-catenin? Based on experimental findings, we develop a compartmental model and conduct several simulation experiments. Our analysis reveals the following key findings: 1) nucleo-cytoplasmic shuttling of β-catenin and its antagonists can yield a spatial separation between the said proteins, which results in a breakdown of β-catenin degradation, followed by an accumulation of β-catenin and hence leads to an increase of the [β-catenin/TCF] concentration. Our results strongly suggest that Wnt signalling can benefit from nucleo-cytoplasmic shuttling of APC, Axin and GSK3, although they are in general β-catenin antagonising proteins. 2) The total robustness of the [β-catenin/TCF] output is closely linked to its absolute concentration levels. We demonstrate that the compartmental separation of β-catenin and the destruction complex does not only lead to a maximization, but additionally to an increased robustness of [β-catenin/TCF] signalling against perturbations in the cellular environment. 3) A nuclear accumulation of the destruction complex renders the pathway robust against fluctuations in Wnt signalling and against changes in the compartmental distribution of β-catenin. 4) Elucidating the impact of

  5. Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants.

    PubMed Central

    Szilvay, A M; Brokstad, K A; Kopperud, R; Haukenes, G; Kalland, K H

    1995-01-01

    The human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev moves repeatedly between the cytoplasm, a perinuclear zone, the nucleoli, and nucleoplasmic speckles. In this study, we demonstrated by both indirect immunofluorescence and Western immunoblot analysis that nuclear exit of Rev transdominant negative mutants was defective compared with that of wild-type Rev. The basic and activation domains of Rev signal import and export, respectively, of Rev across the nuclear membrane. In cotransfection experiments, mutants containing mutations of Rev inhibited the nuclear egress of wild-type Rev, thus revealing a novel transdominant negative phenotype. PMID:7745679

  6. Nucleocytoplasmic Shuttling of Valosin-Containing Protein (VCP/p97) Regulated by Its N domain and C-terminal Region

    PubMed Central

    Song, Changcheng; Wang, Qing; Song, Changzheng; Lockett, Stephen J.; Colburn, Nancy H.; Li, Chou-Chi H.; Wang, Ji Ming; Rogers, Thomas J.

    2014-01-01

    Valosin-containing protein (VCP or p97), a member of AAA family (ATPases associated with diverse cellular activities), plays a key role in many important cellular activities. A genetic deficiency of VCP can cause inclusion body myopathy associated with Paget’s disease of bone and frontotemporal dementia (IBMPFD). Previous studies showed that the VCP N domain is essential for the regulation of nuclear entry of VCP. Here we report that IBMPFD mutations, which are mainly located in the N domain, suppress the nuclear entry of VCP. Moreover, the peptide sequence G780AGPSQ in the C-terminal region regulates the retention of VCP in the nucleus. A mutant lacking this sequence can increase the nuclear distribution of IBMPFD VCP, suggesting that this sequence is a potential molecular target for correcting the deficient nucleocytoplasmic shuttling of IBMPFD VCP proteins. PMID:25447673

  7. Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei

    PubMed Central

    Lichius, Alexander; Seidl-Seiboth, Verena; Seiboth, Bernhard; Kubicek, Christian P

    2014-01-01

    Trichoderma reesei is a model for investigating the regulation of (hemi-)cellulase gene expression. Cellulases are formed adaptively, and the transcriptional activator XYR1 and the carbon catabolite repressor CRE1 are main regulators of their expression. We quantified the nucleo-cytoplasmic shuttling dynamics of GFP-fusion proteins of both transcription factors under cellulase and xylanase inducing conditions, and correlated their nuclear presence/absence with transcriptional changes. We also compared their subcellular localization in conidial germlings and mature hyphae. We show that cellulase gene expression requires de novo biosynthesis of XYR1 and its simultaneous nuclear import, whereas carbon catabolite repression is regulated through preformed CRE1 imported from the cytoplasmic pool. Termination of induction immediately stopped cellulase gene transcription and was accompanied by rapid nuclear degradation of XYR1. In contrast, nuclear CRE1 rapidly decreased upon glucose depletion, and became recycled into the cytoplasm. In mature hyphae, nuclei containing activated XYR1 were concentrated in the colony center, indicating that this is the main region of XYR1 synthesis and cellulase transcription. CRE1 was found to be evenly distributed throughout the entire mycelium. Taken together, our data revealed novel aspects of the dynamic shuttling and spatial bias of the major regulator of (hemi-)cellulase gene expression, XYR1, in T. reesei. PMID:25302561

  8. Extracellular signal-regulated kinase 2 (ERK-2) mediated phosphorylation regulates nucleo-cytoplasmic shuttling and cell growth control of Ras-associated tumor suppressor protein, RASSF2

    SciTech Connect

    Kumari, Gita; Mahalingam, S.

    2009-10-01

    Ras GTPase controls the normal cell growth through binding with an array of effector molecules, such as Raf and PI3-kinase in a GTP-dependent manner. RASSF2, a member of the Ras association domain family, is known to be involved in the suppression of cell growth and is frequently down-regulated in various tumor tissues by promoter hypermethylation. In the present study, we demonstrate that RASSF2 shuttles between nucleus and cytoplasm by a signal-mediated process and its export from the nucleus is sensitive to leptomycin B. Amino acids between 240 to 260 in the C-terminus of RASSF2 harbor a functional nuclear export signal (NES), which is necessary and sufficient for efficient export of RASSF2 from the nucleus. Substitution of conserved Ile254, Val257 and Leu259 within the minimal NES impaired RASSF2 export from the nucleus. In addition, wild type but not the nuclear export defective RASSF2 mutant interacts with export receptor, CRM-1 and exported from the nucleus. Surprisingly, we observed nucleolar localization for the nuclear export defective mutant suggesting the possibility that RASSF2 may localize in different cellular compartments transiently in a cell cycle dependent manner and the observed nuclear localization for wild type protein may be due to faster export kinetics from the nucleolus. Furthermore, our data suggest that RASSF2 is specifically phosphorylated by MAPK/ERK-2 and the inhibitors of MAPK pathway impair the phosphorylation and subsequently block the export of RASSF2 from the nucleus. These data clearly suggest that ERK-2 mediated phosphorylation plays an important role in regulating the nucleo-cytoplasmic shuttling of RASSF2. Interestingly, nuclear import defective mutant of RASSF2 failed to induce cell cycle arrest at G1/S phase and apoptosis suggesting that RASSF2 regulates cell growth in a nuclear localization dependent manner. Collectively, these data provided evidence for the first time that MAPK/ERK-2 mediated phosphorylation regulates

  9. Rho-kinase signaling controls nucleocytoplasmic shuttling of class IIa Histone Deacetylase (HDAC7) and transcriptional activation of orphan nuclear receptor NR4A1

    SciTech Connect

    Compagnucci, Claudia; Barresi, Sabina; Petrini, Stefania; Bertini, Enrico; Zanni, Ginevra

    2015-04-03

    Rho-kinase (ROCK) has been well documented to play a key role in RhoA-induced actin remodeling. ROCK activation results in myosin light chain (MLC) phosphorylation either by direct action on MLC kinase (MLCK) or by inhibition of MLC phosphatase (MLCP), modulating actin–myosin contraction. We found that inhibition of the ROCK pathway in induced pluripotent stem cells, leads to nuclear export of HDAC7 and transcriptional activation of the orphan nuclear receptor NR4A1 while in cells with constitutive ROCK hyperactivity due to loss of function of the RhoGTPase activating protein Oligophrenin-1 (OPHN1), the orphan nuclear receptor NR4A1 is downregulated. Our study identify a new target of ROCK signaling via myosin phosphatase subunit (MYPT1) and Histone Deacetylase (HDAC7) at the nuclear level and provide new insights in the cellular functions of ROCK. - Highlights: • ROCK regulates nucleocytoplasmic shuttling of HDAC7 via phosphorylation of MYPT1. • Nuclear export of HDAC7 and upregulation of NR4A1 occurs with low ROCK activity. • High levels of ROCK activity due to OPHN1 loss of function downregulate NR4A1.

  10. A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling.

    PubMed

    Blanc, Valerie; Kennedy, Susan; Davidson, Nicholas O

    2003-10-17

    C to U editing of the nuclear apolipoprotein B (apoB) transcript is mediated by a core enzyme containing a catalytic deaminase, apobec-1, and an RNA binding subunit, apobec-1 complementation factor (ACF). ACF expression is predominantly nuclear, including mutant proteins with deletions of a putative nuclear localization signal. We have now identified a novel 41-residue motif (ANS) in the auxiliary domain of ACF that functions as an authentic nuclear localization signal. ANS-green fluorescence protein and ANS-beta-galactosidase chimeras were both expressed exclusively in the nucleus, whereas wild-type chimeras or an ACF deletion mutant lacking the ANS were cytoplasmic. Nuclear accumulation of ACF is transcription-dependent, temperature-sensitive, and reversible, features reminiscent of a shuttling protein. ACF relocates to the cytoplasm after actinomycin D treatment, an effect blocked by the CRM1 inhibitor leptomycin B. Heterokaryon assays confirmed directly that ACF shuttles in vivo. ACF binds to the protein carrier, transportin 2 in vivo, and colocalizes to the nucleus as determined by confocal microscopy. Co-immunoprecipitation experiments revealed that transportin 2 binds directly to the ANS motif. These data suggest that directed nuclear localization and compartmentalization of the core complex of the apoB RNA editing enzyme is regulated through a dominant targeting sequence (ANS) contained within ACF. PMID:12896982

  11. The human actin-related protein hArp5: Nucleo-cytoplasmic shuttling and involvement in DNA repair

    SciTech Connect

    Kitayama, Kumiko; Kamo, Mariko; Oma, Yukako; Matsuda, Ryo; Uchida, Takafumi; Ikura, Tsuyoshi; Tashiro, Satoshi; Ohyama, Takashi; Winsor, Barbara; Harata, Masahiko

    2009-01-15

    Certain actin-related proteins (Arps) of budding yeast are localized in the nucleus, and have essential roles as stoichiometric components of histone acetyltransferase (HAT) and chromatin remodeling complexes. On the other hand, identification of vertebrate nuclear Arps and their functional analyses are just beginning. We show that human Arp5 (hArp5) proteins are localized in the nucleus, and that arp5{delta} yeast cells are partially complemented by hArp5. Thus, hArp5 is a novel member of the nuclear Arps of vertebrates, which possess evolutionarily conserved functions from yeast to humans. We show here that hArp5 shuttles between the nucleus and the cytoplasm. Furthermore, after the induction of DNA double strand breaks (DSB), cell growth and the accumulation of phosphorylated histone H2AX ({gamma}-H2AX) are impaired by hArp5 depletion. Association of hArp5 with the hIno80 chromatin remodeling enzyme and decrease of chromatin-bound hIno80 by hArp5-depletion indicate that hArp5 may have a role in the recruitment of the hINO80 complex to chromatin. Overexpression of hArp5 and hIno80 enhanced {gamma}-H2AX accumulation. These observations suggest that hArp5 is involved in the process of DSB repair through the regulation of the chromatin remodelling machinery.

  12. Introduction to nucleocytoplasmic transport: molecules and mechanisms.

    PubMed

    Peters, Reiner

    2006-01-01

    Nucleocytoplasmic transport, the exchange of matter between nucleus and cytoplasm, plays a fundamental role in human and other eukaryotic cells, affecting almost every aspect of health and disease. The only gate for the transport of small and large molecules as well as supramolecular complexes between nucleus and cytoplasm is the nuclear pore complex (NPC). The NPC is not a normal membrane transport protein (transporter). Composed of 500 to 1000 peptide chains, the NPC features a mysterious functional duality. For most molecules, it constitutes a molecular sieve with a blurred cutoff at approx 10 nm, but for molecules binding to phenylalanine-glycine (FG) motifs, the NPC appears to be a channel of approx 50 nm diameter, permitting bidirectional translocation at high speed. To achieve this, the NPC cooperates with soluble factors, the nuclear transport receptors, which shuttle between nuclear contents and cytoplasm. Here, we provide a short introduction to nucleocytoplasmic transport by describing first the structure and composition of the nuclear pore complex. Then, mechanisms of nucleocytoplasmic transport are discussed. Finally, the still essentially unresolved mechanisms by which nuclear transport receptors and transport complexes are translocated through the nuclear pore complex are considered, and a novel translocation model is suggested. PMID:16739728

  13. Nucleocytoplasmic transport of macromolecules.

    PubMed Central

    Corbett, A H; Silver, P A

    1997-01-01

    Nucleocytoplasmic transport is a complex process that consists of the movement of numerous macromolecules back and forth across the nuclear envelope. All macromolecules that move in and out of the nucleus do so via nuclear pore complexes that form large proteinaceous channels in the nuclear envelope. In addition to nuclear pores, nuclear transport of macromolecules requires a number of soluble factors that are found both in the cytoplasm and in the nucleus. A combination of biochemical, genetic, and cell biological approaches have been used to identify and characterize the various components of the nuclear transport machinery. Recent studies have shown that both import to and export from the nucleus are mediated by signals found within the transport substrates. Several studies have demonstrated that these signals are recognized by soluble factors that target these substrates to the nuclear pore. Once substrates have been directed to the pore, most transport events depend on a cycle of GTP hydrolysis mediated by the small Ras-like GTPase, Ran, as well as other proteins that regulate the guanine nucleotide-bound state of Ran. Many of the essential factors have been identified, and the challenge that remains is to determine the exact mechanism by which transport occurs. This review attempts to present an integrated view of our current understanding of nuclear transport while highlighting the contributions that have been made through studies with genetic organisms such as the budding yeast, Saccharomyces cerevisiae. PMID:9184010

  14. Centrosome-associated regulators of the G2/M checkpoint as targets for cancer therapy

    PubMed Central

    Wang, Yingmei; Ji, Ping; Liu, Jinsong; Broaddus, Russell R; Xue, Fengxia; Zhang, Wei

    2009-01-01

    In eukaryotic cells, control mechanisms have developed that restrain cell-cycle transitions in response to stress. These regulatory pathways are termed cell-cycle checkpoints. The G2/M checkpoint prevents cells from entering mitosis when DNA is damaged in order to afford these cells an opportunity to repair the damaged DNA before propagating genetic defects to the daughter cells. If the damage is irreparable, checkpoint signaling might activate pathways that lead to apoptosis. Since alteration of cell-cycle control is a hallmark of tumorigenesis, cell-cycle regulators represent potential targets for therapy. The centrosome has recently come into focus as a critical cellular organelle that integrates G2/M checkpoint control and repairs signals in response to DNA damage. A growing number of G2/M checkpoint regulators have been found in the centrosome, suggesting that centrosome has an important role in G2/M checkpoint function. In this review, we discuss centrosome-associated regulators of the G2/M checkpoint, the dysregulation of this checkpoint in cancer, and potential candidate targets for cancer therapy. PMID:19216791

  15. Functional insights of nucleocytoplasmic transport in plants

    PubMed Central

    Tamura, Kentaro; Hara-Nishimura, Ikuko

    2014-01-01

    Plant nucleocytoplasmic transport beyond the nuclear envelope is important not only for basic cellular functions but also for growth, development, hormonal signaling, and responses to environmental stimuli. Key components of this transport system include nuclear transport receptors and nucleoporins. The functional and physical interactions between receptors and the nuclear pore in the nuclear membrane are indispensable for nucleocytoplasmic transport. Recently, several groups have reported various plant mutants that are deficient in factors involved in nucleocytoplasmic transport. Here, we summarize the current state of knowledge about nucleocytoplasmic transport in plants, and we review the plant-specific regulation and roles of this process in plants. PMID:24765097

  16. Viral Subversion of Nucleocytoplasmic Trafficking

    PubMed Central

    Yarbrough, Melanie L.; Mata, Miguel A.; Sakthivel, Ramanavelan; Fontoura, Beatriz M. A.

    2014-01-01

    Trafficking of proteins and RNA into and out of the nucleus occurs through the nuclear pore complex (NPC). Due to its critical function in many cellular processes, the NPC and transport factors are common targets of several viruses that disrupt key constituents of the machinery to facilitate viral replication. Many viruses such as poliovirus and severe acute respiratory syndrome (SARS) virus inhibit protein import into the nucleus, while viruses such as influenza A virus target and disrupt host mRNA nuclear export. Current evidence indicates that these viruses may employ such strategies to avert the host immune response. Conversely, many viruses co-opt nucleocytoplasmic trafficking to facilitate transport of viral RNAs. Since viral proteins interact with key regulators of the host nuclear transport machinery, viruses have served as invaluable tools of discovery that led to the identification of novel constituents of nuclear transport pathways. In addition, this review explores the importance of nucleocytoplasmic trafficking to viral pathogenesis as these studies revealed new antiviral therapeutic strategies and exposed previously unknown cellular mechanisms. Further understanding of nuclear transport pathways will determine whether such therapeutics will be useful treatments for important human pathogens. PMID:24289861

  17. Inherited human sex reversal due to impaired nucleocytoplasmic trafficking of SRY defines a male transcriptional threshold

    PubMed Central

    Chen, Yen-Shan; Racca, Joseph D.; Phillips, Nelson B.; Weiss, Michael A.

    2013-01-01

    Human testis determination is initiated by SRY (sex determining region on Y chromosome). Mutations in SRY cause gonadal dysgenesis with female somatic phenotype. Two subtle variants (V60L and I90M in the high-mobility group box) define inherited alleles shared by an XY sterile daughter and fertile father. Whereas specific DNA binding and bending are unaffected in a rat embryonic pre-Sertoli cell line, the variants exhibited selective defects in nucleocytoplasmic shuttling due to impaired nuclear import (V60L; mediated by Exportin-4) or export (I90M; mediated by chromosome region maintenance 1). Decreased shuttling limits nuclear accumulation of phosphorylated (activated) SRY, in turn reducing occupancy of DNA sites regulating Sertoli-cell differentiation [the testis-specific SRY-box 9 (Sox9) enhancer]. Despite distinct patterns of biochemical and cell-biological perturbations, V60L and I90M each attenuated Sox9 expression in transient transfection assays by twofold. Such attenuation was also observed in studies of V60A, a clinical variant associated with ovotestes and hence ambiguity between divergent cell fates. This shared twofold threshold is reminiscent of autosomal syndromes of transcription-factor haploinsufficiency, including XY sex reversal associated with mutations in SOX9. Our results demonstrate that nucleocytoplasmic shuttling of SRY is necessary for robust initiation of testicular development. Although also characteristic of ungulate orthologs, such shuttling is not conserved among rodents wherein impaired nuclear export of the high-mobility group box and import-dependent phosphorylation are compensated by a microsatellite-associated transcriptional activation domain. Human sex reversal due to subtle defects in the nucleocytoplasmic shuttling of SRY suggests that its transcriptional activity lies near the edge of developmental ambiguity. PMID:24003159

  18. A Nucleocytoplasmic Shuttling Protein in Oxidative Stress Tolerance

    SciTech Connect

    Ow, David W.; Song, Wen

    2003-03-26

    Plants for effective extraction of toxic metals and radionuclides must tolerate oxidative stress. To identify genes that enhance oxidative stress tolerance, an S. pombe cDNA expression plasmid library was screened for the ability to yield hypertolerant colonies. Here, we report on the properties of one gene that confers hypertolerance to cadmium and oxidizing chemicals. This gene appears to be conserved in other organisms as homologous genes are found in human, mouse, fruitfly and Arabidopsis. The fruitfly and Arabidopsis genes likewise enhance oxidative stress tolerance in fission yeast. During oxidative stress, the amount of mRNA does not change, but protein fusions to GFP relocate from the cytoplasm to the nucleus. The same pattern is observed with the Arabidopsis homologue-GFP fusion protein. This behavior suggests a signaling role in oxidative stress tolerance and these conserved proteins may be targets for engineering stress tolerant plants for phytoremediation.

  19. Nucleocytoplasmic shuttling of hexokinase II in a cancer cell

    SciTech Connect

    Neary, Catherine L.; Pastorino, John G.

    2010-04-16

    In yeast, the hexokinase type II enzyme (HXKII) translocates to the nucleus in the presence of excess glucose, and participates in glucose repression. However, no evidence has suggested a nuclear function for HXKII in mammalian cells. Herein, we present data showing nuclear localization of HXKII in HeLa cells, both by immunocytochemistry and subcellular fractionation. HXKII is extruded from the nucleus, at least in part, by the activity of the exportin 1/CrmA system, as demonstrated by increased nuclear expression and decreased cytoplasmic expression after incubation with leptomycin B, a bacterially-derived exportin inhibitor. Furthermore, cytoplasmic localization of HXKII is dependent on its enzymatic activity, as inhibiting HXKII activity using 2-deoxy-D-glucose (2DG) increased nuclear localization. This effect was more significant in cells incubated in the absence of glucose for 24 h prior to addition of 2DG. Regulated translocation of HXKII to the nucleus of mammalian cells could represent a previously unknown glucose-sensing mechanism.

  20. The Effects of Modeled Microgravity on Nucleocytoplasmic Localization of Human Apurinic/Apyrimidinic

    NASA Technical Reports Server (NTRS)

    Gonda, Steve; Jackson, E.B.

    2004-01-01

    Exposure to space radiation and microgravity occurs to humans during space flight. In order to have accurate risk estimations, answering questions to whether increased DNA damage seen during space flight in modified by microgravity are important. Several studies have examined whether intercellular repair of radiation-induced DNA lesions are modified by microgravity. Results from these studies show no modification of the repair processes due to microgravity. However, it is known that in studies not involving radiation that microgravity interferes with normal development. Interestingly, there is no data that attempts to analyze the possible effects of microgravity on the trafficking of DNA repair proteins. In this study, we analyze the effects of modeled microgravity on nucleocytoplasmic shuttling of the human DNA repair enzyme apurinic/apyrimidinic endonuclease 1 (APE1/Ref1) which is involved in base excision repair. We examined nuclear translocation of APE1 using enhanced green fluorescent protein (EGFP) fused to APE1 as a reporter. While APE1 under normal gravity showed normal nuclear localization, APE1 nuclear localization under modeled microgravity was decreased. These results suggest that nucleocytoplasmic translocation of APE1 is modified under modeled microgravity.

  1. Mammalian cells contain a second nucleocytoplasmic hexosaminidase.

    PubMed

    Gutternigg, Martin; Rendić, Dubravko; Voglauer, Regina; Iskratsch, Thomas; Wilson, Iain B H

    2009-04-01

    Some thirty years ago, work on mammalian tissues suggested the presence of two cytosolic hexosaminidases in mammalian cells; one of these has been more recently characterized in a recombinant form and has an important role in cellular function due to its ability to cleave beta-N-acetylglucosamine residues from a variety of nuclear and cytoplasmic proteins. However, the molecular nature of the second cytosolic hexosaminidase, named hexosaminidase D, has remained obscure. In the present study, we molecularly characterize for the first time the human and murine recombinant forms of enzymes, encoded by HEXDC genes, which appear to correspond to hexosaminidase D in terms of substrate specificity, pH dependency and temperature stability. Furthermore, a Myc-tagged form of this novel hexosaminidase displays a nucleocytoplasmic localization. Transcripts of the corresponding gene are expressed in a number of murine tissues. On the basis of its sequence, this enzyme represents, along with the lysosomal hexosaminidase subunits encoded by the HEXA and HEXB genes, the third class 20 glycosidase to be identified from mammalian sources. PMID:19040401

  2. Nucleoporins and nucleocytoplasmic transport in hematologic malignancies.

    PubMed

    Takeda, Akiko; Yaseen, Nabeel R

    2014-08-01

    Hematologic malignancies are often associated with chromosomal rearrangements that lead to the expression of chimeric fusion proteins. Rearrangements of the genes encoding two nucleoporins, NUP98 and NUP214, have been implicated in the pathogenesis of several types of hematologic malignancies, particularly acute myeloid leukemia. NUP98 rearrangements result in fusion of an N-terminal portion of NUP98 to one of numerous proteins. These rearrangements often follow treatment with topoisomerase II inhibitors and tend to occur in younger patients. They have been shown to induce leukemia in mice and to enhance proliferation and disrupt differentiation in primary human hematopoietic precursors. NUP214 has only a few fusion partners. DEK-NUP214 is the most common NUP214 fusion in AML; it tends to occur in younger patients and is usually associated with FLT3 internal tandem duplications. The leukemogenic activity of NUP214 fusions is less well characterized. Normal nucleoporins, including NUP98 and NUP214, have important functions in nucleocytoplasmic transport, transcription, and mitosis. These functions and their disruptions by oncogenic nucleoporin fusions are discussed. PMID:24657637

  3. Mechanism of Human Nucleocytoplasmic Hexosaminidase D

    PubMed Central

    2016-01-01

    Mammalian β-hexosaminidases have been shown to play essential roles in cellular physiology and health. These enzymes are responsible for the cleavage of the monosaccharides N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) from cellular substrates. One of these β-hexosaminidases, hexosaminidase D (HexD), encoded by the HEXDC gene, has received little attention. No mechanistic studies have focused on the role of this unusual nucleocytoplasmically localized β-hexosaminidase, and its cellular function remains unknown. Using a series of kinetic and mechanistic investigations into HexD, we define the precise catalytic mechanism of this enzyme and establish the identities of key enzymic residues. The preparation of synthetic aryl N-acetylgalactosaminide substrates for HexD in combination with measurements of kinetic parameters for wild-type and mutant enzymes, linear free energy analyses of the enzyme-catalyzed hydrolysis of these substrates, evaluation of the reaction by nuclear magnetic resonance, and inhibition studies collectively reveal the detailed mechanism of action employed by HexD. HexD is a retaining glycosidase that operates using a substrate-assisted catalytic mechanism, has a preference for galactosaminide over glucosaminide substrates, and shows a pH optimum in its second-order rate constant at pH 6.5–7.0. The catalytically important residues are Asp148 and Glu149, with Glu149 serving as the general acid/base residue and Asp148 as the polarizing residue. HexD is inhibited by Gal-NAG-thiazoline (Ki = 420 nM). The fundamental insights gained from this study will aid in the development of potent and selective probes for HexD, which will serve as useful tools to improve our understanding of the physiological role played by this unusual enzyme. PMID:27149221

  4. Mechanism of Human Nucleocytoplasmic Hexosaminidase D.

    PubMed

    Alteen, Matthew G; Oehler, Verena; Nemčovičová, Ivana; Wilson, Iain B H; Vocadlo, David J; Gloster, Tracey M

    2016-05-17

    Mammalian β-hexosaminidases have been shown to play essential roles in cellular physiology and health. These enzymes are responsible for the cleavage of the monosaccharides N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) from cellular substrates. One of these β-hexosaminidases, hexosaminidase D (HexD), encoded by the HEXDC gene, has received little attention. No mechanistic studies have focused on the role of this unusual nucleocytoplasmically localized β-hexosaminidase, and its cellular function remains unknown. Using a series of kinetic and mechanistic investigations into HexD, we define the precise catalytic mechanism of this enzyme and establish the identities of key enzymic residues. The preparation of synthetic aryl N-acetylgalactosaminide substrates for HexD in combination with measurements of kinetic parameters for wild-type and mutant enzymes, linear free energy analyses of the enzyme-catalyzed hydrolysis of these substrates, evaluation of the reaction by nuclear magnetic resonance, and inhibition studies collectively reveal the detailed mechanism of action employed by HexD. HexD is a retaining glycosidase that operates using a substrate-assisted catalytic mechanism, has a preference for galactosaminide over glucosaminide substrates, and shows a pH optimum in its second-order rate constant at pH 6.5-7.0. The catalytically important residues are Asp148 and Glu149, with Glu149 serving as the general acid/base residue and Asp148 as the polarizing residue. HexD is inhibited by Gal-NAG-thiazoline (Ki = 420 nM). The fundamental insights gained from this study will aid in the development of potent and selective probes for HexD, which will serve as useful tools to improve our understanding of the physiological role played by this unusual enzyme. PMID:27149221

  5. Osmotic stress alters chromatin condensation and nucleocytoplasmic transport

    SciTech Connect

    Finan, John D.; Leddy, Holly A.; Guilak, Farshid

    2011-05-06

    Highlights: {yields} The rate of nucleocytoplasmic transport increases under hyper-osmotic stress. {yields} The mechanism is a change in nuclear geometry, not a change in permeability of the nuclear envelope. {yields} Intracytoplasmic but not intranuclear diffusion is sensitive to osmotic stress. {yields} Pores in the chromatin of the nucleus enlarge under hyper-osmotic stress. -- Abstract: Osmotic stress is a potent regulator of biological function in many cell types, but its mechanism of action is only partially understood. In this study, we examined whether changes in extracellular osmolality can alter chromatin condensation and the rate of nucleocytoplasmic transport, as potential mechanisms by which osmotic stress can act. Transport of 10 kDa dextran was measured both within and between the nucleus and the cytoplasm using two different photobleaching methods. A mathematical model was developed to describe fluorescence recovery via nucleocytoplasmic transport. As osmolality increased, the diffusion coefficient of dextran decreased in the cytoplasm, but not the nucleus. Hyper-osmotic stress decreased nuclear size and increased nuclear lacunarity, indicating that while the nucleus was getting smaller, the pores and channels interdigitating the chromatin had expanded. The rate of nucleocytoplasmic transport was increased under hyper-osmotic stress but was insensitive to hypo-osmotic stress, consistent with the nonlinear osmotic properties of the nucleus. The mechanism of this osmotic sensitivity appears to be a change in the size and geometry of the nucleus, resulting in a shorter effective diffusion distance for the nucleus. These results may explain physical mechanisms by which osmotic stress can influence intracellular signaling pathways that rely on nucleocytoplasmic transport.

  6. SPEED Microscopy and Its Application in Nucleocytoplasmic Transport.

    PubMed

    Ma, Jiong; Kelich, Joseph M; Yang, Weidong

    2016-01-01

    In eukaryotic cells, the nuclear pore complexes (NPCs) selectively mediate the bidirectional trafficking of macromolecules between the cytoplasm and the nucleus. The selective barrier is formed by intrinsically disordered phenylalanine-glycine (FG) nucleoporins anchored on the wall of the submicrometer NPC, which allows for passive diffusion and facilitated translocation through the nuclear pore. Dysfunction of nucleocytoplasmic transport has been associated with many human diseases. However, due to the technical challenge of imaging the native tomography of the FG-nucleoporin barrier and its interactions with transiting molecules in the native NPC, the precise nucleocytoplasmic transport mechanism remains unresolved. To refine the transport mechanism, single-molecule fluorescence microscopy methods have been employed to obtain the transport kinetics and the spatial transport route of individual fluorescent molecules through the NPC. In this method paper, we particularly highlight a newly developed high-speed super-resolution three-dimensional microscopy approach, termed as SPEED (single-point edge-excitation subdiffraction) microscopy, and its application in characterizing nucleocytoplasmic transport. PMID:27147062

  7. Space Shuttle

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The space shuttle flight system and mission profile are briefly described. Emphasis is placed on the economic and social benefits of the space transportation system. The space shuttle vehicle is described in detail.

  8. Space Shuttle.

    ERIC Educational Resources Information Center

    National Aeronautics and Space Administration, Washington, DC.

    The plans for utilizing reusable space shuttles which could replace almost all present expendable launch vehicles are briefly described. Many illustrations are included showing the artists' concepts of various configurations proposed for space shuttles. (PR)

  9. The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.

    PubMed

    Zhang, Ke; Donnelly, Christopher J; Haeusler, Aaron R; Grima, Jonathan C; Machamer, James B; Steinwald, Peter; Daley, Elizabeth L; Miller, Sean J; Cunningham, Kathleen M; Vidensky, Svetlana; Gupta, Saksham; Thomas, Michael A; Hong, Ingie; Chiu, Shu-Ling; Huganir, Richard L; Ostrow, Lyle W; Matunis, Michael J; Wang, Jiou; Sattler, Rita; Lloyd, Thomas E; Rothstein, Jeffrey D

    2015-09-01

    The hexanucleotide repeat expansion (HRE) GGGGCC (G4C2) in C9orf72 is the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Recent studies support an HRE RNA gain-of-function mechanism of neurotoxicity, and we previously identified protein interactors for the G4C2 RNA including RanGAP1. A candidate-based genetic screen in Drosophila expressing 30 G4C2 repeats identified RanGAP (Drosophila orthologue of human RanGAP1), a key regulator of nucleocytoplasmic transport, as a potent suppressor of neurodegeneration. Enhancing nuclear import or suppressing nuclear export of proteins also suppresses neurodegeneration. RanGAP physically interacts with HRE RNA and is mislocalized in HRE-expressing flies, neurons from C9orf72 ALS patient-derived induced pluripotent stem cells (iPSC-derived neurons), and in C9orf72 ALS patient brain tissue. Nuclear import is impaired as a result of HRE expression in the fly model and in C9orf72 iPSC-derived neurons, and these deficits are rescued by small molecules and antisense oligonucleotides targeting the HRE G-quadruplexes. Nucleocytoplasmic transport defects may be a fundamental pathway for ALS and FTD that is amenable to pharmacotherapeutic intervention. PMID:26308891

  10. The C9ORF72 repeat expansion disrupts nucleocytoplasmic transport

    PubMed Central

    Haeusler, Aaron R.; Grima, Jonathan C.; Machamer, James B.; Steinwald, Peter; Daley, Elizabeth L.; Miller, Sean J.; Cunningham, Kathleen M.; Vidensky, Svetlana; Gupta, Saksham; Thomas, Michael A.; Hong, Ingie; Chiu, Shu-Ling; Huganir, Richard L.; Ostrow, Lyle W.; Matunis, Michael J.; Wang, Jiou; Sattler, Rita

    2016-01-01

    A GGGGCC (G4C2) hexanucleotide repeat expansion (HRE) in C9ORF72 is the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Recent studies support an HRE RNA gain-of-function mechanism of neurotoxicity, and we previously identified protein interactors for the G4C2 RNA including RanGAP1. A candidate-based genetic screen in Drosophila expressing 30 G4C2 repeats identified RanGAP (Drosophila ortholog of human RanGAP1), a key regulator of nucleocytoplasmic transport, as a potent suppressor of neurodegeneration. Enhancing nuclear import or suppressing nuclear export of proteins also suppresses neurodegeneration. RanGAP physically interacts with HRE RNA and is mislocalized in HRE-expressing flies, neurons from C9ORF72 ALS patient-derived induced pluripotent stem cells (iPSNs), and in C9ORF72 patient brain tissue. Nuclear import is impaired as a result of HRE expression in the fly model and in C9ORF72 iPSNs, and these deficits are rescued by small molecules and antisense oligonucleotides targeting the HRE G-quadruplexes. Nucleocytoplasmic transport defects may be a fundamental pathway for ALS and FTD amenable to pharmacotherapeutic intervention. PMID:26308891

  11. Problem-Solving Test: Nucleocytoplasmic Shuttling of Pre-mRNA Binding Proteins

    ERIC Educational Resources Information Center

    Szeberenyi, Jozsef

    2012-01-01

    Terms to be familiar with before you start to solve the test: transcription, pre-mRNA, RNA processing, RNA transport, RNA polymerase II, direct and indirect immunofluorescence staining, cell fractionation by centrifugation, oligo(dT)-cellulose chromatography, washing and elution of the column, ribonuclease, SDS-polyacrylamide gel electrophoresis,…

  12. Nucleocytoplasmic shuttling of the HSV-2 serine/threonine kinase Us3

    SciTech Connect

    Finnen, Renee L.; Johnston, Susan M.; Neron, Casey E.; Banfield, Bruce W.

    2011-08-15

    The alphaherpesvirus serine/threonine kinase Us3 plays diverse roles in virus multiplication and modifies both nuclear and cytoplasmic substrates. We recently reported that treatment of HSV-2 Us3-transfected and HSV-2-infected cells with leptomycin B, an inhibitor of nuclear export mediated by interaction of chromosomal regional maintenance protein (CRM1) with leucine rich nuclear export signals (NESs), resulted in nuclear trapping of Us3. Here, we utilized fluorescence loss in photobleaching to monitor nuclear export of HSV-2 Us3 and confirm that this process proceeds solely via a CRM1-mediated mechanism. Analysis of deletion derivatives of HSV-2 Us3 fused to a nuclear export reporter protein implicated the involvement of NES-like sequences in nuclear export. However, nuclear trapping of HSV-2 Us3 proteins carrying mutations in these potential NESs was not observed, indicating that these sequences are not functional in the context of full-length protein. Our analyses also revealed previously unidentified regions of HSV-2 Us3 that contribute to its kinase activity.

  13. Nucleocytoplasmic Transport: A Paradigm for Molecular Logistics in Artificial Systems.

    PubMed

    Vujica, Suncica; Zelmer, Christina; Panatala, Radhakrishnan; Lim, Roderick Y H

    2016-01-01

    Artificial organelles, molecular factories and nanoreactors are membrane-bound systems envisaged to exhibit cell-like functionality. These constitute liposomes, polymersomes or hybrid lipo-polymersomes that display different membrane-spanning channels and/or enclose molecular modules. To achieve more complex functionality, an artificial organelle should ideally sustain a continuous influx of essential macromolecular modules (i.e. cargoes) and metabolites against an outflow of reaction products. This would benefit from the incorporation of selective nanopores as well as specific trafficking factors that facilitate cargo selectivity, translocation efficiency, and directionality. Towards this goal, we describe how proteinaceous cargoes are transported between the nucleus and cytoplasm by nuclear pore complexes and the biological trafficking machinery in living cells (i.e. nucleocytoplasmic transport). On this basis, we discuss how biomimetic control may be implemented to selectively import, compartmentalize and accumulate diverse macromolecular modules against concentration gradients in artificial organelles. PMID:27363369

  14. Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.

    PubMed

    Halim, Adnan; Larsen, Ida Signe Bohse; Neubert, Patrick; Joshi, Hiren Jitendra; Petersen, Bent Larsen; Vakhrushev, Sergey Y; Strahl, Sabine; Clausen, Henrik

    2015-12-22

    Dynamic cycling of N-Acetylglucosamine (GlcNAc) on serine and threonine residues (O-GlcNAcylation) is an essential process in all eukaryotic cells except yeast, including Saccharomyces cerevisiae and Schizosaccharomyces pombe. O-GlcNAcylation modulates signaling and cellular processes in an intricate interplay with protein phosphorylation and serves as a key sensor of nutrients by linking the hexosamine biosynthetic pathway to cellular signaling. A longstanding conundrum has been how yeast survives without O-GlcNAcylation in light of its similar phosphorylation signaling system. We previously developed a sensitive lectin enrichment and mass spectrometry workflow for identification of the human O-linked mannose (O-Man) glycoproteome and used this to identify a pleothora of O-Man glycoproteins in human cell lines including the large family of cadherins and protocadherins. Here, we applied the workflow to yeast with the aim to characterize the yeast O-Man glycoproteome, and in doing so, we discovered hitherto unknown O-Man glycosites on nuclear, cytoplasmic, and mitochondrial proteins in S. cerevisiae and S. pombe. Such O-Man glycoproteins were not found in our analysis of human cell lines. However, the type of yeast O-Man nucleocytoplasmic proteins and the localization of identified O-Man residues mirror that of the O-GlcNAc glycoproteome found in other eukaryotic cells, indicating that the two different types of O-glycosylations serve the same important biological functions. The discovery opens for exploration of the enzymatic machinery that is predicted to regulate the nucleocytoplasmic O-Man glycosylations. It is likely that manipulation of this type of O-Man glycosylation will have wide applications for yeast bioprocessing. PMID:26644575

  15. Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast

    PubMed Central

    Halim, Adnan; Larsen, Ida Signe Bohse; Neubert, Patrick; Joshi, Hiren Jitendra; Petersen, Bent Larsen; Vakhrushev, Sergey Y.; Strahl, Sabine; Clausen, Henrik

    2015-01-01

    Dynamic cycling of N-Acetylglucosamine (GlcNAc) on serine and threonine residues (O-GlcNAcylation) is an essential process in all eukaryotic cells except yeast, including Saccharomyces cerevisiae and Schizosaccharomyces pombe. O-GlcNAcylation modulates signaling and cellular processes in an intricate interplay with protein phosphorylation and serves as a key sensor of nutrients by linking the hexosamine biosynthetic pathway to cellular signaling. A longstanding conundrum has been how yeast survives without O-GlcNAcylation in light of its similar phosphorylation signaling system. We previously developed a sensitive lectin enrichment and mass spectrometry workflow for identification of the human O-linked mannose (O-Man) glycoproteome and used this to identify a pleothora of O-Man glycoproteins in human cell lines including the large family of cadherins and protocadherins. Here, we applied the workflow to yeast with the aim to characterize the yeast O-Man glycoproteome, and in doing so, we discovered hitherto unknown O-Man glycosites on nuclear, cytoplasmic, and mitochondrial proteins in S. cerevisiae and S. pombe. Such O-Man glycoproteins were not found in our analysis of human cell lines. However, the type of yeast O-Man nucleocytoplasmic proteins and the localization of identified O-Man residues mirror that of the O-GlcNAc glycoproteome found in other eukaryotic cells, indicating that the two different types of O-glycosylations serve the same important biological functions. The discovery opens for exploration of the enzymatic machinery that is predicted to regulate the nucleocytoplasmic O-Man glycosylations. It is likely that manipulation of this type of O-Man glycosylation will have wide applications for yeast bioprocessing. PMID:26644575

  16. Shuttle Reference Data

    NASA Astrophysics Data System (ADS)

    2002-12-01

    This collection of shuttle reference data contains the following information: shuttle abort history, shuttle abort modes, abort decisions, space shuttle rendezvous maneuvers, space shuttle main engines, space shuttle solid rocket boosters, hold-down posts, SRB (solid rocket boosters) ignition, electrical power distribution, hydraulic power units, thrust vector control, SBR rate gyro assemblies, SBR separation and Space Shuttle Super Super Light Weight Tank (SLWT).

  17. Space Shuttle

    NASA Technical Reports Server (NTRS)

    1975-01-01

    A general description of the space shuttle program is presented, with emphasis on its application to the use of space for commercial, scientific, and defense needs. The following aspects of the program are discussed: description of the flight system (orbiter, external tank, solid rocket boosters) and mission profile, direct benefits related to life on earth (both present and expected), description of the space shuttle vehicle and its associated supporting systems, economic impacts (including indirect benefits such as lower inflation rates), listing of participating organizations.

  18. Space Shuttle

    NASA Technical Reports Server (NTRS)

    Roberts, Lisa

    2005-01-01

    This viewgraph presentation summarizes changes to the Space Shuttle Propulsions Systems made for the Return to Flight in response to the recommendations of the Columbia Accident Investigation Board (CAIB). The presentation also includes an overivew of the Columbia debris recovery effort.

  19. Respiratory virus modulation of host nucleocytoplasmic transport; target for therapeutic intervention?

    PubMed Central

    Caly, Leon; Ghildyal, Reena; Jans, David A.

    2015-01-01

    The respiratory diseases caused by rhinovirus, respiratory syncytial virus, and influenza virus represent a large social and financial burden on healthcare worldwide. Although all three viruses have distinctly unique properties in terms of infection and replication, they share the ability to exploit/manipulate the host-cell nucleocytoplasmic transport system in order to replicate effectively and efficiently. This review outlines the various ways in which infection by these viruses impacts on the host nucleocytoplasmic transport system, and examples where inhibition thereof in turn decreases viral replication. The highly conserved nature of the nucleocytoplasmic transport system and the viral proteins that interact with it make this virus–host interface a prime candidate for the development of specific antiviral therapeutics in the future. PMID:26322040

  20. Shuttle Processing

    NASA Technical Reports Server (NTRS)

    Guodace, Kimberly A.

    2010-01-01

    This slide presentation details shuttle processing flow which starts with wheel stop and ends with launching. The flow is from landing the orbiter is rolled into the Orbiter Processing Facility (OPF), where processing is performed, it is then rolled over to the Vehicle Assembly Building (VAB) where it is mated with the propellant tanks, and payloads are installed. A different flow is detailed if the weather at Kennedy Space Center requires a landing at Dryden.

  1. Perturbation of nucleo-cytoplasmic transport affects size of nucleus and nucleolus in human cells.

    PubMed

    Ganguly, Abira; Bhattacharjee, Chumki; Bhave, Madhura; Kailaje, Vaishali; Jain, Bhawik K; Sengupta, Isha; Rangarajan, Annapoorni; Bhattacharyya, Dibyendu

    2016-03-01

    Size regulation of human cell nucleus and nucleolus are poorly understood subjects. 3D reconstruction of live image shows that the karyoplasmic ratio (KR) increases by 30-80% in transformed cell lines compared to their immortalized counterpart. The attenuation of nucleo-cytoplasmic transport causes the KR value to increase by 30-50% in immortalized cell lines. Nucleolus volumes are significantly increased in transformed cell lines and the attenuation of nucleo-cytoplasmic transport causes a significant increase in the nucleolus volume of immortalized cell lines. A cytosol and nuclear fraction swapping experiment emphasizes the potential role of unknown cytosolic factors in nuclear and nucleolar size regulation. PMID:26813731

  2. The Shuttle Era

    NASA Technical Reports Server (NTRS)

    1981-01-01

    An overview of the Space Shuttle Program is presented. The missions of the space shuttle orbiters, the boosters and main engine, and experimental equipment are described. Crew and passenger accommodations are discussed as well as the shuttle management teams.

  3. Shuttle Era: Launch Directors

    NASA Video Gallery

    A space shuttle launch director is the leader of the complex choreography that goes into a shuttle liftoff. Ten people have served as shuttle launch directors, making the final decision whether the...

  4. HIV-1 Recruits UPF1 but Excludes UPF2 to Promote Nucleocytoplasmic Export of the Genomic RNA

    PubMed Central

    Ajamian, Lara; Abel, Karen; Rao, Shringar; Vyboh, Kishanda; García-de-Gracia, Francisco; Soto-Rifo, Ricardo; Kulozik, Andreas E.; Gehring, Niels H.; Mouland, Andrew J.

    2015-01-01

    Unspliced, genomic HIV-1 RNA (vRNA) is a component of several ribonucleoprotein complexes (RNP) during the viral replication cycle. In earlier work, we demonstrated that the host upframeshift protein 1 (UPF1), a key factor in nonsense-mediated mRNA decay (NMD), colocalized and associated to the viral structural protein Gag during viral egress. In this work, we demonstrate a new function for UPF1 in the regulation of vRNA nuclear export. We establish that the nucleocytoplasmic shuttling of UPF1 is required for this function and demonstrate that UPF1 exists in two essential viral RNPs during the late phase of HIV-1 replication: the first, in a nuclear export RNP that contains Rev, CRM1, DDX3 and the nucleoporin p62, and the second, which excludes these nuclear export markers but contains Gag in the cytoplasm. Interestingly, we observed that both UPF2 and the long isoform of UPF3a, UPF3aL, but not the shorter isoforms UPF3aS and UPF3b, are excluded from the UPF1-Rev-CRM1-DDX3 complex as they are negative regulators of vRNA nuclear export. In silico protein-protein docking analyses suggest that Rev binds UPF1 in a region that overlaps the UPF2 binding site, thus explaining the exclusion of this negative regulatory factor by HIV-1 that is necessary for vRNA trafficking. This work uncovers a novel and unique regulatory circuit involving several UPF proteins that ultimately regulate vRNA nuclear export and trafficking. PMID:26492277

  5. HIV-1 Recruits UPF1 but Excludes UPF2 to Promote Nucleocytoplasmic Export of the Genomic RNA.

    PubMed

    Ajamian, Lara; Abel, Karen; Rao, Shringar; Vyboh, Kishanda; García-de-Gracia, Francisco; Soto-Rifo, Ricardo; Kulozik, Andreas E; Gehring, Niels H; Mouland, Andrew J

    2015-01-01

    Unspliced, genomic HIV-1 RNA (vRNA) is a component of several ribonucleoprotein complexes (RNP) during the viral replication cycle. In earlier work, we demonstrated that the host upframeshift protein 1 (UPF1), a key factor in nonsense-mediated mRNA decay (NMD), colocalized and associated to the viral structural protein Gag during viral egress. In this work, we demonstrate a new function for UPF1 in the regulation of vRNA nuclear export. OPEN ACCESS Biomolecules 2015, 5 2809 We establish that the nucleocytoplasmic shuttling of UPF1 is required for this function and demonstrate that UPF1 exists in two essential viral RNPs during the late phase of HIV-1 replication: the first, in a nuclear export RNP that contains Rev, CRM1, DDX3 and the nucleoporin p62, and the second, which excludes these nuclear export markers but contains Gag in the cytoplasm. Interestingly, we observed that both UPF2 and the long isoform of UPF3a, UPF3aL, but not the shorter isoforms UPF3aS and UPF3b, are excluded from the UPF1-Rev-CRM1-DDX3 complex as they are negative regulators of vRNA nuclear export. In silico protein-protein docking analyses suggest that Rev binds UPF1 in a region that overlaps the UPF2 binding site, thus explaining the exclusion of this negative regulatory factor by HIV-1 that is necessary for vRNA trafficking. This work uncovers a novel and unique regulatory circuit involving several UPF proteins that ultimately regulate vRNA nuclear export and trafficking. PMID:26492277

  6. A Shuttle evolution strategy

    NASA Technical Reports Server (NTRS)

    Teixeira, Charles; Mallini, Charles

    1989-01-01

    An overview of a potential Space Shuttle evolution strategy is presented. A Shuttle development study which reviews past and ongoing studies, implements a Shuttle Enhancement Data Base, and develops a methodology and a strawman evolution strategy is discussed. The long-term goals of a Shuttle evolution strategy, including increased reliability, lower cost, robustness, resiliency, increased capability, and assured access are addressed.

  7. Cytoplasmic protein aggregates interfere with nucleocytoplasmic transport of protein and RNA.

    PubMed

    Woerner, Andreas C; Frottin, Frédéric; Hornburg, Daniel; Feng, Li R; Meissner, Felix; Patra, Maria; Tatzelt, Jörg; Mann, Matthias; Winklhofer, Konstanze F; Hartl, F Ulrich; Hipp, Mark S

    2016-01-01

    Amyloid-like protein aggregation is associated with neurodegeneration and other pathologies. The nature of the toxic aggregate species and their mechanism of action remain elusive. Here, we analyzed the compartment specificity of aggregate toxicity using artificial β-sheet proteins, as well as fragments of mutant huntingtin and TAR DNA binding protein-43 (TDP-43). Aggregation in the cytoplasm interfered with nucleocytoplasmic protein and RNA transport. In contrast, the same proteins did not inhibit transport when forming inclusions in the nucleus at or around the nucleolus. Protein aggregation in the cytoplasm, but not the nucleus, caused the sequestration and mislocalization of proteins containing disordered and low-complexity sequences, including multiple factors of the nuclear import and export machinery. Thus, impairment of nucleocytoplasmic transport may contribute to the cellular pathology of various aggregate deposition diseases. PMID:26634439

  8. The Importin β Binding Domain as a Master Regulator of Nucleocytoplasmic Transport

    PubMed Central

    Lott, Kaylen; Cingolani, Gino

    2010-01-01

    Specific and efficient recognition of import cargoes is essential to ensure nucleocytoplasmic transport. To this end, the prototypical karyopherin importin β associates with import cargoes directly or, more commonly, through import adaptors, such as importin α and snurportin. Adaptor proteins bind the nuclear localization sequence (NLS) of import cargoes while recruiting importin β via an N-terminal importin β binding (IBB) domain. The use of adaptors greatly expands and amplifies the repertoire of cellular cargoes that importin β can efficiently import into the cell nucleus and allows for fine regulation of nuclear import. Accordingly, the IBB-domain is a dedicated NLS, unique to adaptor proteins that functions as a molecular liaison between importin β and import cargoes. This review provides an overview of the molecular role played by the IBB-domain in orchestrating nucleocytoplasmic transport. Recent work has determined that the IBB-domain has specialized functions at every step of the import and export pathway. Unexpectedly, this stretch of ∼40 amino acids plays an essential role in regulating processes such as formation of the import complex, docking and translocation through the nuclear pore complex (NPC), release of import cargoes into the cell nucleus and finally recycling of import adaptors and importin β into the cytoplasm. Thus, the IBB-domain is a master regulator of nucleocytoplasmic transport, whose complex molecular function is only recently beginning to emerge. PMID:21029753

  9. Nucleo-cytoplasmic transport as a therapeutic target of cancer.

    PubMed

    Gravina, Giovanni Luca; Senapedis, William; McCauley, Dilara; Baloglu, Erkan; Shacham, Sharon; Festuccia, Claudio

    2014-01-01

    Shuttling of specific proteins out of the nucleus is essential for the regulation of the cell cycle and proliferation of both normal and malignant tissues. Dysregulation of this fundamental process may affect many other important cellular processes such as tumor growth, inflammatory response, cell cycle, and apoptosis. It is known that XPO1 (Exportin-1/Chromosome Region Maintenance 1/CRM1) is the main mediator of nuclear export in many cell types. Nuclear proteins exported to the cytoplasm by XPO1 include the drug targets topoisomerase IIα (topo IIα) and BCR-ABL and tumor suppressor proteins such as Rb, APC, p53, p21, and p27. XPO1 can mediate cell proliferation through several pathways: (i) the sub-cellular localization of NES-containing oncogenes and tumor suppressor proteins, (ii) the control of the mitotic apparatus and chromosome segregation, and (iii) the maintenance of nuclear and chromosomal structures. The XPO1 protein is elevated in ovarian carcinoma, glioma, osteosarcoma, pancreatic and cervical cancer. There is a growing body of research indicating that XPO1 may have an important role as a prognostic marker in solid tumors. Because of this, nuclear export inhibition through XPO1 is a potential target for therapeutic intervention in many cancers. The best understood XPO1 inhibitors are the small molecule nuclear export inhibitors (NEIs; Leptomycin B and derivatives, ratjadones, PKF050-638, valtrate, ACA, CBS9106, selinexor/KPT-330, and verdinexor/KPT-335). Selinexor and verdinexor are orally bioavailable, highly potent, small molecules that are classified as Selective Inhibitors of Nuclear Export (SINE). KPT-330 is the only NEI currently in Phase I/II human clinical trials in hematological and solid cancers. Of all the potential targets in nuclear cytoplasmic transport, the nuclear export receptor XPO1 remains the best understood and most advanced therapeutic target for the treatment of cancer. PMID:25476752

  10. Nucleocytoplasmic shuttling of STK16 (PKL12), a Golgi-resident serine/threonine kinase involved in VEGF expression regulation

    SciTech Connect

    Guinea, Barbara; Gonzalez de la Pena, Manuel . E-mail: abernad@cnb.uam.es

    2006-01-15

    PKL12/STK16 protein is the first identified mammalian member of a ser/thr kinase subfamily that is conserved across several kingdoms, with a broad expression pattern in murine tissues and cell types. Endogenous STK16 subcellular localization was evaluated by indirect immunofluorescence in NIH/3T3 and NRK cells, demonstrating a Golgi-associated pattern that appears to be independent of signals provided by integrin pathways. When cells were treated with brefeldin A (BFA) or nocodazole, drugs that promote Golgi disorganization, we observed STK16 translocation to the nuclear compartment. Constitutive overexpression of this protein by retroviral vectors also promotes accumulation of STK16 in the nuclear compartment, as shown by subfractionation studies. A kinase-dead STK16 mutant (E202A) was used to demonstrate that both the Golgi association and the nuclear translocation capabilities seem to be independent of the STK16 kinase activity. In addition, we show that STK16 overexpression in several cell lines enhances their capacity to produce and secrete VEGF. To confirm these data in vivo, we injected tumor cells overexpressing STK16 into immunodeficient BALBc/SCID mice. HT1080-derived tumors overexpressing STK16 showed increased volume and number of blood vessels compared to controls. Altogether, these data concur with previous reports suggesting a potential role for STK16 as a transcriptional co-activator.

  11. Secondhand smoke exposure-induced nucleocytoplasmic shuttling of HMGB1 in a rat premature skin aging model.

    PubMed

    Chaichalotornkul, Sirintip; Nararatwanchai, Thamthiwat; Narkpinit, Somphong; Dararat, Pornpen; Kikuchi, Kiyoshi; Maruyama, Ikuro; Tancharoen, Salunya

    2015-01-01

    Secondhand cigarette smoke exposure (SSE) has been linked to carcinogenic, oxidative, and inflammatory reactions. Herein, we investigated whether premature skin aging could be induced by SSE in a rat model, and assessed the cytoplasmic translocation of high mobility group box 1 (HMGB1) protein and collagen loss in skin tissues. Animals were divided into two groups: SSE and controls. Whole body SSE was carried out for 12 weeks. Dorsal skin tissue specimens were harvested for HMGB1 and Mallory's azan staining. Correlations between serum HMGB1 and collagen levels were determined. Rat skin exposed to secondhand smoke lost collagen bundles in the papillary dermis and collagen decreased significantly (p<0.05) compared with control rats. In epidermal keratinocytes, cytoplasmic HMGB1 staining was more diffuse and there were more HMGB1-positive cells after four weeks in SSE compared to control rats. A negative correlation between HMGB1 serum and collagen levels (r=-0.631, p=0.28) was also observed. Therefore, cytoplasmic HMGB1 expression in skin tissues might be associated with skin collagen loss upon the initiation of SSE. Additionally, long-term SSE might affect the appearance of the skin, or could accelerate the skin aging process. PMID:25446104

  12. Shuttle Landing Facility

    NASA Video Gallery

    The Shuttle Landing Facility at NASA's Kennedy Space Center in Florida marked the finish line for space shuttle missions since 1984. It is also staffed by a group of air traffic controllers who wor...

  13. Shuttle interaction study extension

    NASA Technical Reports Server (NTRS)

    1982-01-01

    The following areas of Space Shuttle technology were discussed: variable altitude strategy, spacecraft servicing, propellant storage, orbiter plume impingement, space based design, mating (docking and berthing), shuttle fleet utilization, and mission/traffic model.

  14. Shuttle Showcase: Firsts

    NASA Video Gallery

    The space shuttle has defined an era and broken boundaries both in space and on Earth. Among the hundreds of people who have flown on the shuttle, many have been firsts -- for their race, their cou...

  15. NASA Facts, Space Shuttle.

    ERIC Educational Resources Information Center

    National Aeronautics and Space Administration, Washington, DC. Educational Programs Div.

    This newsletter from the National Aeronautics and Space Administration (NASA) contains a description of the purposes and potentials of the Space Shuttle craft. The illustrated document explains some of the uses for which the shuttle is designed; how the shuttle will be launched from earth, carry out its mission, and land again on earth; and what a…

  16. Shuttle plate braiding machine

    NASA Technical Reports Server (NTRS)

    Huey, Jr., Cecil O. (Inventor)

    1994-01-01

    A method and apparatus for moving yarn in a selected pattern to form a braided article. The apparatus includes a segmented grid of stationary support elements and a plurality of shuttles configured to carry yarn. The shuttles are supported for movement on the grid assembly and each shuttle includes a retractable plunger for engaging a reciprocating shuttle plate that moves below the grid assembly. Such engagement at selected times causes the shuttles to move about the grid assembly in a selected pattern to form a braided article of a particular geometry.

  17. An integrated model for the nucleo-cytoplasmic transport of cytoplasmic poly(A)-binding proteins.

    PubMed

    Burgess, Hannah M; Gray, Nicola K

    2012-05-01

    Cytoplasmic poly(A)-binding proteins (PABPs) regulate mRNA stability and translation. Although predominantly localized in the cytoplasm, PABP proteins also cycle through the nucleus. Recent work has established that their steady-state localization can be altered by cellular stresses such as ultraviolet (UV) radiation, and infection by several viruses, resulting in nuclear accumulation of PABPs. Here, we present further evidence that their interaction with and release from mRNA and translation complexes are important in determining their sub-cellular distribution and propose an integrated model for regulated nucleo-cytoplasmic transport of PABPs. PMID:22896784

  18. Three cardiovirus Leader proteins equivalently inhibit four different nucleocytoplasmic trafficking pathways

    SciTech Connect

    Ciomperlik, Jessica J.; Basta, Holly A.; Palmenberg, Ann C.

    2015-10-15

    Cardiovirus infections inhibit nucleocytoplasmic trafficking by Leader protein-induced phosphorylation of Phe/Gly-containing nucleoporins (Nups). Recombinant Leader from encephalomyocarditis virus, Theiler's murine encephalomyelitis virus and Saffold virus target the same subset of Nups, including Nup62 and Nup98, but not Nup50. Reporter cell lines with fluorescence mCherry markers for M9, RS and classical SV40 import pathways, as well as the Crm1-mediated export pathway, all responded to transfection with the full panel of Leader proteins, showing consequent cessation of path-specific active import/export. For this to happen, the Nups had to be presented in the context of intact nuclear pores and exposed to cytoplasmic extracts. The Leader phosphorylation cascade was not effective against recombinant Nup proteins. The findings support a model of Leader-dependent Nup phosphorylation with the purpose of disrupting Nup-transportin interactions. - Highlights: • Nup98, but not Nup50 becomes phosphorylated by cardiovirus Leader protein-dependent mechanisms. • At least four independent nucleocytoplasmic trafficking pathways are inhibited by this process. • Nups must be presented in a nuclear pore context for Leader-directed phosphorylation. • Leader, by itself, does not cause activation of cellular kinases.

  19. Tumor marker nucleoporin 88 kDa regulates nucleocytoplasmic transport of NF-{kappa}B

    SciTech Connect

    Takahashi, Nozomi Kilsdonk, Jeroen W.J. van; Ostendorf, Benedikt; Smeets, Ruben; Bruggeman, Sophia W.M.; Alonso, Angel; Loo, Fons van de; Schneider, Matthias; Berg, Wim B. van den; Swart, Guido W.M.

    2008-09-26

    Nucleoporin 88 kDa (Nup88) is a tumor marker, overexpressed in various types of cancer. In Drosophila Nup88 (mbo) was reported to selectively mediate the nucleocytoplasmic transport of NF-{kappa}B, an ubiquitous transcription factor involved in immune responses, apoptosis, and cancer. We addressed the function of Nup88 in mammalian cells. Selective depletion of Nup88 by small interfering RNA (siRNA) inhibited NF-{kappa}B-dependent reporter gene activation and the nuclear translocation of NF-{kappa}B without affecting the upstream activation pathway in NIH3T3 cells. In contrast, nuclear translocation of glucocorticoid receptor was not reduced by the depletion of Nup88. In metastatic melanoma cells overexpressing Nup88, constitutive activation of NF-{kappa}B was found both in nucleus and cytoplasm. Nup88 depletion in these cells reduced TNF-induced nuclear accumulation of NF-{kappa}B subunits. We conclude that Nup88 regulates the activity of NF-{kappa}B at the level of nucleocytoplasmic transport. Overexpression of Nup88 in tumor cells may, thus be involved in the constitutive NF-{kappa}B activation.

  20. Shuttle communications design study

    NASA Technical Reports Server (NTRS)

    Cartier, D. E.

    1975-01-01

    The design and development of a space shuttle communication system are discussed. The subjects considered include the following: (1) Ku-band satellite relay to shuttle, (2) phased arrays, (3) PN acquisition, (4) quadriplexing of direct link ranging and telemetry, (5) communications blackout on launch and reentry, (6) acquisition after blackout on reentry, (7) wideband communications interface with the Ku-Band rendezvous radar, (8) aeroflight capabilities of the space shuttle, (9) a triple multiplexing scheme equivalent to interplex, and (10) a study of staggered quadriphase for use on the space shuttle.

  1. Potential Future Shuttle Improvements

    NASA Technical Reports Server (NTRS)

    Henderson, Edward

    2001-01-01

    NASA has just recently completed the 104th flight of the Space Shuttle. Each of the four Orbiters in the Shuttle fleet have a design life of 100 flights each. Thus the fleet is capable of almost 300 more flights, and at current flight rates could potentially operate well past 2020 if necessary. This paper addresses some of the potential Shuttle system improvements that could be considered if the decision is made to continue operations of this vehicle for such an extended period. The national space transportation policy envisions a decision around 2005-2006 concerning readiness to start development of a Shuttle replacement system. Leading up to that decision point NASA is investing in the Space Launch Initiative (SLI) to reduce the development risks associated with key technologies needed for the next generation reusable launch vehicle (RLV). The Shuttle replacement could be a new design RLV or could be based on a Shuttle derived design: i.e., a vehicle based on the current Shuttle but with major design changes. The technology investment strategy of SLI is supportive of either approach. However, if NASA and industry are not ready to develop a replacement vehicle in the 2006-2012 timeframe, then another option would be to continue to make important, but evolutionary changes, to the existing Shuttle fleet. The overall strategy for next generation RLV planning, including possible Shuttle evolution, is captured in Figure 1.

  2. Shuttle Wastewater Solution Characterization

    NASA Technical Reports Server (NTRS)

    Adam, Niklas; Pham, Chau

    2011-01-01

    During the 31st shuttle mission to the International Space Station, STS-129, there was a clogging event in the shuttle wastewater tank. A routine wastewater dump was performed during the mission and before the dump was completed, degraded flow was observed. In order to complete the wastewater dump, flow had to be rerouted around the dump filter. As a result, a basic chemical and microbial investigation was performed to understand the shuttle wastewater system and perform mitigation tasks to prevent another blockage. Testing continued on the remaining shuttle flights wastewater and wastewater tank cleaning solutions. The results of the analyses and the effect of the mitigation steps are detailed in this paper.

  3. Nuclear Shuttle in Flight

    NASA Technical Reports Server (NTRS)

    1970-01-01

    This 1970 artist's concept shows a Nuclear Shuttle in flight. As envisioned by Marshall Space Flight Center Program Development engineers, the Nuclear Shuttle would deliver payloads to lunar orbit or other destinations then return to Earth orbit for refueling and additional missions.

  4. Stennis tests shuttle valves

    NASA Technical Reports Server (NTRS)

    2009-01-01

    Flames burst from the E-1 Test Stand as Stennis Space Center engineers perform one of dozens of shuttle flow valve tests in early February. Stennis engineers teamed with Innovative Partnership Program partners to perform the tests after NASA officials delayed the launch of the STS-119 mission because of concerns with the shuttle part.

  5. Autonomous Space Shuttle

    NASA Technical Reports Server (NTRS)

    Siders, Jeffrey A.; Smith, Robert H.

    2004-01-01

    The continued assembly and operation of the International Space Station (ISS) is the cornerstone within NASA's overall Strategic P an. As indicated in NASA's Integrated Space Transportation Plan (ISTP), the International Space Station requires Shuttle to fly through at least the middle of the next decade to complete assembly of the Station, provide crew transport, and to provide heavy lift up and down mass capability. The ISTP reflects a tight coupling among the Station, Shuttle, and OSP programs to support our Nation's space goal . While the Shuttle is a critical component of this ISTP, there is a new emphasis for the need to achieve greater efficiency and safety in transporting crews to and from the Space Station. This need is being addressed through the Orbital Space Plane (OSP) Program. However, the OSP is being designed to "complement" the Shuttle as the primary means for crew transfer, and will not replace all the Shuttle's capabilities. The unique heavy lift capabilities of the Space Shuttle is essential for both ISS, as well as other potential missions extending beyond low Earth orbit. One concept under discussion to better fulfill this role of a heavy lift carrier, is the transformation of the Shuttle to an "un-piloted" autonomous system. This concept would eliminate the loss of crew risk, while providing a substantial increase in payload to orbit capability. Using the guidelines reflected in the NASA ISTP, the autonomous Shuttle a simplified concept of operations can be described as; "a re-supply of cargo to the ISS through the use of an un-piloted Shuttle vehicle from launch through landing". Although this is the primary mission profile, the other major consideration in developing an autonomous Shuttle is maintaining a crew transportation capability to ISS as an assured human access to space capability.

  6. Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans.

    PubMed

    Askjaer, Peter; Galy, Vincent; Meister, Peter

    2014-01-01

    The nematode Caenorhabditis elegans is characterized by many features that make it highly attractive to study nuclear pore complexes (NPCs) and nucleocytoplasmic transport. NPC composition and structure are highly conserved in nematodes and being amenable to a variety of genetic manipulations, key aspects of nuclear envelope dynamics can be observed in great details during breakdown, reassembly, and interphase. In this chapter, we provide an overview of some of the most relevant modern techniques that allow researchers unfamiliar with C. elegans to embark on studies of nucleoporins in an intact organism through its development from zygote to aging adult. We focus on methods relevant to generate loss-of-function phenotypes and their analysis by advanced microscopy. Extensive references to available reagents, such as mutants, transgenic strains, and antibodies are equally useful to scientists with or without prior C. elegans or nucleoporin experience. PMID:24857735

  7. Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly

    PubMed Central

    Lucena, Rafael; Dephoure, Noah; Gygi, Steve P.; Kellogg, Douglas R.; Tallada, Victor A.

    2015-01-01

    During each cell cycle, the mitotic spindle is efficiently assembled to achieve chromosome segregation and then rapidly disassembled as cells enter cytokinesis. Although much has been learned about assembly, how spindles disassemble at the end of mitosis remains unclear. Here we demonstrate that nucleocytoplasmic transport at the membrane domain surrounding the mitotic spindle midzone, here named the midzone membrane domain (MMD), is essential for spindle disassembly in Schizosaccharomyces pombe cells. We show that, during anaphase B, Imp1-mediated transport of the AAA-ATPase Cdc48 protein at the MMD allows this disassembly factor to localize at the spindle midzone, thereby promoting spindle midzone dissolution. Our findings illustrate how a separate membrane compartment supports spindle disassembly in the closed mitosis of fission yeast. PMID:25963819

  8. Nucleocytoplasmic Recycling of the Nuclear Localization Signal Receptor α Subunit In Vivo Is Dependent on a Nuclear Export Signal, Energy, and RCC1

    PubMed Central

    Boche, Irene; Fanning, Ellen

    1997-01-01

    Nuclear protein import requires a nuclear localization signal (NLS) receptor and at least three other cytoplasmic factors. The α subunit of the NLS receptor, Rag cohort 1 (Rch1), enters the nucleus, probably in a complex with the β subunit of the receptor, as well as other import factors and the import substrate. To learn more about which factors and/or events end the import reaction and how the import factors return to the cytoplasm, we have studied nucleocytoplasmic shuttling of Rch1 in vivo. Recombinant Rch1 microinjected into Vero or tsBN2 cells was found primarily in the cytoplasm. Rch1 injected into the nucleus was rapidly exported in a temperature-dependent manner. In contrast, a mutant of Rch1 lacking the first 243 residues accumulated in the nuclei of Vero cells after cytoplasmic injection. After nuclear injection, the truncated Rch1 was retained in the nucleus, but either Rch1 residues 207–217 or a heterologous nuclear export signal, but not a mutant form of residues 207–217, restored nuclear export. Loss of the nuclear transport factor RCC1 (regulator of chromosome condensation) at the nonpermissive temperature in the thermosensitive mutant cell line tsBN2 caused nuclear accumulation of wild-type Rch1 injected into the cytoplasm. However, free Rch1 injected into nuclei of tsBN2 cells at the nonpermissive temperature was exported. These results suggested that RCC1 acts at an earlier step in Rch1 recycling, possibly the disassembly of an import complex that contains Rch1 and the import substrate. Consistent with this possibility, incubation of purified RanGTP and RCC1 with NLS receptor and import substrate prevented assembly of receptor/substrate complexes or stimulated their disassembly. PMID:9334337

  9. Space shuttle requirements/configuration evolution

    NASA Technical Reports Server (NTRS)

    Andrews, E. P.

    1991-01-01

    Space Shuttle chronology; Space Shuttle comparison; Cost comparison; Performance; Program ground rules; Sizing criteria; Crew/passenger provisions; Space Shuttle Main Engine (SSME) characteristics; Space Shuttle program milestones; and Space Shuttle requirements are outlined. This presentation is represented by viewgraphs.

  10. [Importin-β1 plays a key role in the nucleocytoplasmic transportation process of MARVELD1].

    PubMed

    Wang, Z Y; Shi, M; Li, Y

    2015-01-01

    While MARVEL domain-containing protein 1 (MARVELD1) is widely expressed in normal tissue, its expression is decreased in tumor tissue. Importin-β1 is a kind of nucleocytoplasmic transport protein, which participates in protein transportation between the nucleus and cytoplasm. It has been known that MARVELD1 binds importin-β1. To define the biological functions of the binding between MARVELD1 and importin-β1, confocal laser scanning microscopy was applied to confirm the distribution of MARVELD1 in the cells when importin-β1, is knocked down, and the results show that MARVELD1's concentration is decreased in the nucleus and elevated in the cytoplasm. GST-pull down and western blot were applied to test the binding affinity between the normal importin-β1, and several truncations of MARVELD1, and the binding affinity between the normal MARVELD1 and several truncations of importin-β1. These results show that there exist several binding points between MARVELD1 and importin-β1. Basing on these results one can come to a conclusion that importin-β1 mediates the non-classical nucleocytoplasmic transportation of MARVELD1. It was also found that knockdown of importin-β1, leads to an increase in the total density of MARVELD1. Considering the abnormally reduced expression of MARVELD1 in tumor tissue, the results of this study will help gaining a better understanding of the roles of MARVELD1 in the signal network systems during tumorigenesis in cancer cells. PMID:26107903

  11. Regulation of nucleocytoplasmic trafficking of transcription factor OREBP/TonEBP/NFAT5.

    PubMed

    Tong, Edith H Y; Guo, Jin-Jun; Huang, Ai-Long; Liu, Han; Hu, Chang-Deng; Chung, Stephen S M; Ko, Ben C B

    2006-08-18

    The osmotic response element-binding protein (OREBP), also known as tonicity enhancer-binding protein (TonEBP) or NFAT5, regulates the hypertonicity-induced expression of a battery of genes crucial for the adaptation of mammalian cells to extracellular hypertonic stress. The activity of OREBP/TonEBP is regulated at multiple levels, including nucleocytoplasmic trafficking. OREBP/TonEBP protein can be detected in both the cytoplasm and nucleus under isotonic conditions, although it accumulates exclusively in the nucleus or cytoplasm when subjected to hypertonic or hypotonic challenges, respectively. Using immunocytochemistry and green fluorescent protein fusions, the protein domains that determine its subcellular localization were identified and characterized. We found that OREBP/TonEBP nuclear import is regulated by a nuclear localization signal. However, under isotonic conditions, nuclear export of OREBP/TonEBP is mediated by a CRM1-dependent, leucine-rich canonical nuclear export sequence (NES) located in the N terminus. Disruption of NES by site-directed mutagenesis yielded a mutant OREBP/TonEBP protein that accumulated in the nucleus under isotonic conditions but remained a target for hypotonicity-induced nuclear export. More importantly, a putative auxiliary export domain distal to the NES was identified. Disruption of the auxiliary export domain alone is sufficient to abolish the nuclear export of OREBP/TonEBP induced by hypotonicity. By using bimolecular fluorescence complementation assay, we showed that CRM1 interacts with OREBP/TonEBP, but not with a mutant protein deficient in NES. Our findings provide insight into how nucleocytoplasmic trafficking of OREBP/TonEBP is regulated by changes in extracellular tonicity. PMID:16782704

  12. Shuttle Safety Improvements

    NASA Technical Reports Server (NTRS)

    Henderson, Edward

    2001-01-01

    The Space Shuttle has been flying for over 20 years and based on the Orbiter design life of 100 missions it should be capable of flying at least 20 years more if we take care of it. The Space Shuttle Development Office established in 1997 has identified those upgrades needed to keep the Shuttle flying safely and efficiently until a new reusable launch vehicle (RLV) is available to meet the agency commitments and goals for human access to space. The upgrade requirements shown in figure 1 are to meet the program goals, support HEDS and next generation space transportation goals while protecting the country 's investment in the Space Shuttle. A major review of the shuttle hardware and processes was conducted in 1999 which identified key shuttle safety improvement priorities, as well as other system upgrades needed to reliably continue to support the shuttle miss ions well into the second decade of this century. The high priority safety upgrades selected for development and study will be addressed in this paper.

  13. Space Shuttle Abort Evolution

    NASA Technical Reports Server (NTRS)

    Henderson, Edward M.; Nguyen, Tri X.

    2011-01-01

    This paper documents some of the evolutionary steps in developing a rigorous Space Shuttle launch abort capability. The paper addresses the abort strategy during the design and development and how it evolved during Shuttle flight operations. The Space Shuttle Program made numerous adjustments in both the flight hardware and software as the knowledge of the actual flight environment grew. When failures occurred, corrections and improvements were made to avoid a reoccurrence and to provide added capability for crew survival. Finally some lessons learned are summarized for future human launch vehicle designers to consider.

  14. Shuttle mask floorplanning

    NASA Astrophysics Data System (ADS)

    Xu, Gang; Tian, Ruiqi; Wong, Martin D.; Reich, Alfred J.

    2003-12-01

    A shuttle mask has different chips on the same mask. The chips are not electrically connected. Alliance and foundry customers can utilize shuttle masks to share the rising cost of mask and wafer manufacturing. This paper studies the shuttle mask floorplan problem, which is formulated as a rectangle-packing problem with constraints of final die sawing strategy and die-to-die mask inspection. For our formulation, we offer a "merging" method that reduces the problem to an unconstrained slicing floorplan problem. Excellent results are obtained from the experiment with real industry data. We also study a "general" method and discuss the reason why it does not work very well.

  15. Space Shuttle redesign status

    NASA Technical Reports Server (NTRS)

    Brand, Vance D.

    1986-01-01

    NASA has conducted an extensive redesign effort for the Space Shutle in the aftermath of the STS 51-L Challenger accident, encompassing not only Shuttle vehicle and booster design but also such system-wide factors as organizational structure, management procedures, flight safety, flight operations, sustainable flight rate, and maintenance safeguards. Attention is presently given to Solid Rocket Booster redesign features, the Shuttle Main Engine's redesigned high pressure fuel and oxidizer turbopumps, the Shuttle Orbiter's braking and rollout (landing gear) system, the entry control mode of the flight control system, a 'split-S' abort maneuver for the Orbiter, and crew escape capsule proposals.

  16. Shuttle Shaping Up

    NASA Technical Reports Server (NTRS)

    Covault, Craig

    2004-01-01

    Measures to sharply curtail ana document potentially fatal launch debris similar to that which doomed Columbia and her crew should allow the space shuttle to resume flights as early as May. But it could take up to two years before a fully certified thermal protection system and wing leading edge in-orbit repair capability is ready, shuttle managers say. The overall positive safety tradeoffs, however, are enabling return-to-flight preparations to accelerate this month toward making a serious run at launching the shuttle back to space by spring.

  17. The Space Shuttle

    NASA Technical Reports Server (NTRS)

    Moffitt, William L.

    2003-01-01

    As missions have become increasingly more challenging over the years, the most adaptable and capable element of space shuttle operations has proven time and again to be human beings. Human space flight provides unique aspects of observation. interaction and intervention that can reduce risk and improve mission success. No other launch vehicle - in development or in operation today - can match the space shuttle's human space flight capabilities. Preserving U.S. leadership in human space flight requires a strategy to meet those challenges. The ongoing development of next generation vehicles, along with upgrades to the space shuttle, is the most effective means for assuring our access to space.

  18. MSFC shuttle lightning research

    NASA Technical Reports Server (NTRS)

    Vaughan, Otha H., Jr.

    1993-01-01

    The shuttle mesoscale lightning experiment (MLE), flown on earlier shuttle flights, and most recently flown on the following space transportation systems (STS's), STS-31, -32, -35, -37, -38, -40, -41, and -48, has continued to focus on obtaining additional quantitative measurements of lightning characteristics and to create a data base for use in demonstrating observation simulations for future spaceborne lightning mapping systems. These flights are also providing design criteria data for the design of a proposed shuttle MLE-type lightning research instrument called mesoscale lightning observational sensors (MELOS), which are currently under development here at MSFC.

  19. Space Shuttle: The Renewed Promise.

    ERIC Educational Resources Information Center

    McAleer, Neil

    This booklet describes the history of the space shuttle, especially after the Challenger accident. Topics include: (1) "Introduction"; (2) "Return to Flight: The Recovery"; (3) "Space Shuttle Chronology"; (4) "Examples of Other Modifications on Shuttle's Major Systems"; (5) "Space Shuttle Recovery Chronology"; (6) "Poised for Launch: Space Shuttle…

  20. Shuttle/GPSPAC experimentation study

    NASA Technical Reports Server (NTRS)

    Moses, J.; Flack, J. F.

    1977-01-01

    The utilization is discussed of the GPSPAC, which is presently being developed to be used on the low altitude host vehicle (LAHV), for possible use in the shuttle avionics system to evaluate shuttle/GPS navigation performance. Analysis and tradeoffs of the shuttle/GPS link, shuttle signal interface requirements, oscillator tradeoffs and GPSPAC mechanical modifications for shuttle are included. Only the on-orbit utilization of GPSPAC for the shuttle is discussed. Other phases are briefly touched upon. Recommendations are provided for using the present GPSPAC and the changes required to perform shuttle on-orbit navigation.

  1. Shuttle Astronauts Play Chess

    NASA Video Gallery

    STS-134 astronauts Greg Johnson and Greg Chamitoff ponder their next move for the Earth vs. Space chess match. The shuttle crew members also discuss their activities aboard the International Space ...

  2. Habitability study shuttle orbiter

    NASA Technical Reports Server (NTRS)

    1973-01-01

    Habitability design concepts for the Shuttle Orbiter Program are provided for MSC. A variety of creative solutions for the stated tasks are presented. Sketches, mock-ups, mechanicals and models are included for establishing a foundation for future development.

  3. Habitability study shuttle orbiter

    NASA Technical Reports Server (NTRS)

    1972-01-01

    Studies of the habitability of the space shuttle orbiter are briefly summarized. Selected illustrations and descriptions are presented for: crew compartment, hygiene facilities, food system and galley, and storage systems.

  4. Space Shuttle Drawing

    NASA Technical Reports Server (NTRS)

    2004-01-01

    The Apollo program demonstrated that men could travel into space, perform useful tasks there, and return safely to Earth. But space had to be more accessible. This led to the development of the Space Shuttle. The Shuttle's major components are the orbiter spacecraft; the three main engines, with a combined thrust of more than 1.2 million pounds; the huge external tank (ET) that feeds the liquid hydrogen fuel and liquid oxygen oxidizer to the three main engines; and the two solid rocket boosters (SRBs), with their combined thrust of some 5.8 million pounds, that provide most of the power for the first two minutes of flight. Crucially involved with the Space Shuttle program virtually from its inception, the Marshall Space Flight Center (MSFC) played a leading role in the design, development, testing, and fabrication of many major Shuttle propulsion components.

  5. Shuttle car loading system

    NASA Technical Reports Server (NTRS)

    Collins, E. R., Jr. (Inventor)

    1985-01-01

    A system is described for loading newly mined material such as coal, into a shuttle car, at a location near the mine face where there is only a limited height available for a loading system. The system includes a storage bin having several telescoping bin sections and a shuttle car having a bottom wall that can move under the bin. With the bin in an extended position and filled with coal the bin sections can be telescoped to allow the coal to drop out of the bin sections and into the shuttle car, to quickly load the car. The bin sections can then be extended, so they can be slowly filled with more while waiting another shuttle car.

  6. Shuttle Showcase: STS-125

    NASA Video Gallery

    After four previous trips to repair and upgrade the Hubble Space Telescope, it was time for the Shuttle to make one final service call to install new, advanced instruments, batteries, gyros and ins...

  7. Shuttle Inventory Management

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Inventory Management System (SIMS) consists of series of integrated support programs providing supply support for both Shuttle program and Kennedy Space Center base opeations SIMS controls all supply activities and requirements from single point. Programs written in COBOL.

  8. Space Shuttle astrodynamical constants

    NASA Technical Reports Server (NTRS)

    Cockrell, B. F.; Williamson, B.

    1978-01-01

    Basic space shuttle astrodynamic constants are reported for use in mission planning and construction of ground and onboard software input loads. The data included here are provided to facilitate the use of consistent numerical values throughout the project.

  9. Space shuttle revitalization system

    NASA Technical Reports Server (NTRS)

    Quattrone, P. D.

    1985-01-01

    The Space Shuttle air revitalization system is discussed. The sequential steps in loop closure are examined and a schematic outline of the regenerative air revitalization system is presented. Carbon dioxide reduction subsystem concepts are compared. Schemes are drawn for: static feedwater electrolysis cell, solid polymer electrolyte water electrolysis cell, air revitalization system, nitrogen generation reactions, nitrogen subsystem staging, vapor compression distillation subsystem, thermoelectric integrated membrane evaporation subsystem, catalytic distillation water reclamation subsystem, and space shuttle solid waste management system.

  10. Shuttle derived atmosphere

    NASA Technical Reports Server (NTRS)

    Findlay, John

    1987-01-01

    The shuttle descends along a rather shallow path, thus providing some information on the horizontal structure of the atmosphere. Small scale structures were suggested (shears, potholes). The best estimates of the shuttle drag coefficient and projected areas are used to go from accelerometer data to density through the use of BET's (Best Estimated Trajectories). Data are from the IMU's (Inertial Measurement Unit) and the HiRAP (High Resolution Accelerometer Package).

  11. A novel nucleo-cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp

    PubMed Central

    2011-01-01

    Background Unisexual vertebrates have been demonstrated to reproduce by gynogenesis, hybridogenesis, parthenogenesis, or kleptogenesis, however, it is uncertain how the reproduction mode contributes to the clonal diversity. Recently, polyploid gibel carp has been revealed to possess coexisting dual modes of unisexual gynogenesis and sexual reproduction and to have numerous various clones. Using sexual reproduction mating between clone D female and clone A male and subsequent 7 generation multiplying of unisexual gynogenesis, we have created a novel clone strain with more than several hundred millions of individuals. Here, we attempt to identify genetic background of the novel clone and to explore the significant implication for clonal diversity contribution. Methods Several nuclear genome markers and one cytoplasmic marker, the mitochondrial genome sequence, were used to identify the genetic organization of the randomly sampled individuals from different generations of the novel clone. Results Chromosome number, Cot-1 repetitive DNA banded karyotype, microsatellite patterns, AFLP profiles and transferrin alleles uniformly indicated that nuclear genome of the novel clone is identical to that of clone A, and significantly different from that of clone D. However, the cytoplasmic marker, its complete mtDNA genome sequence, is same to that of clone D, and different from that of clone A. Conclusions The present data indicate that the novel clone is a nucleo-cytoplasmic hybrid between the known clones A and D, because it originates from the offspring of gonochoristic sexual reproduction mating between clone D female and clone A male, and contains an entire nuclear genome from the paternal clone A and a mtDNA genome (cytoplasm) from the maternal clone D. It is suggested to arise via androgenesis by a mechanism of ploidy doubling of clone A sperm in clone D ooplasm through inhibiting the first mitotic division. Significantly, the selected nucleo-cytoplasmic hybrid female

  12. 7. YOSEMITE VALLEY SHUTTLE BUS AT SENTINEL BRIDGE SHUTTLE BUS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. YOSEMITE VALLEY SHUTTLE BUS AT SENTINEL BRIDGE SHUTTLE BUS AND PARKING LOT AREA. LOOKING WNW. GIS: N-37 40 36.2 / W-119 44 45.0 - Yosemite National Park Roads & Bridges, Yosemite Village, Mariposa County, CA

  13. Space Shuttle ice nuclei

    NASA Technical Reports Server (NTRS)

    Turco, R. P.; Toon, O. B.; Whitten, R. C.; Cicerone, R. J.

    1982-01-01

    Estimates are made showing that, as a consequence of rocket activity in the earth's upper atmosphere in the Shuttle era, average ice nuclei concentrations in the upper atmosphere could increase by a factor of two, and that an aluminum dust layer weighing up to 1000 tons might eventually form in the lower atmosphere. The concentrations of Space Shuttle ice nuclei (SSIN) in the upper troposphere and lower stratosphere were estimated by taking into account the composition of the particles, the extent of surface poisoning, and the size of the particles. Calculated stratospheric size distributions at 20 km with Space Shuttle particulate injection, calculated SSIN concentrations at 10 and 20 km altitude corresponding to different water vapor/ice supersaturations, and predicted SSIN concentrations in the lower stratosphere and upper troposphere are shown.

  14. Shuttle: forever young?

    PubMed

    Sietzen, Frank

    2002-01-01

    NASA has started a 4-phase program of upgrades designed to increase safety and extend use of the space shuttles through the year 2020. Phase I is aimed at improving vehicle safety and supporting the space station. Phase II is aimed at combating obsolescence and includes a checkout launch and control system and protection from micrometeoroids and orbital debris. Phase III is designed to expand or enhance the capabilities of the shuttle and includes development of an auxiliary power unit, avionics, a channel-wall nozzle, extended nose landing gear, long-life fuel cells, a nontoxic orbital maneuvering system/reaction control system, and a water membrane evaporator. Phase IV is aimed at design of system changes that would alter the shuttle mold line and configuration; projects include a five-segment solid rocket booster, liquid flyback boosters, and a crew escape module. PMID:11794337

  15. Identification of an l-Rhamnose Synthetic Pathway in Two Nucleocytoplasmic Large DNA Viruses▿

    PubMed Central

    Parakkottil Chothi, Madhu; Duncan, Garry A.; Armirotti, Andrea; Abergel, Chantal; Gurnon, James R.; Van Etten, James L.; Bernardi, Cinzia; Damonte, Gianluca; Tonetti, Michela

    2010-01-01

    Nucleocytoplasmic large DNA viruses (NCLDVs) are characterized by large genomes that often encode proteins not commonly found in viruses. Two species in this group are Acanthocystis turfacea chlorella virus 1 (ATCV-1) (family Phycodnaviridae, genus Chlorovirus) and Acanthamoeba polyphaga mimivirus (family Mimiviridae), commonly known as mimivirus. ATCV-1 and other chlorovirus members encode enzymes involved in the synthesis and glycosylation of their structural proteins. In this study, we identified and characterized three enzymes responsible for the synthesis of the sugar l-rhamnose: two UDP-d-glucose 4,6-dehydratases (UGDs) encoded by ATCV-1 and mimivirus and a bifunctional UDP-4-keto-6-deoxy-d-glucose epimerase/reductase (UGER) from mimivirus. Phylogenetic analysis indicated that ATCV-1 probably acquired its UGD gene via a recent horizontal gene transfer (HGT) from a green algal host, while an earlier HGT event involving the complete pathway (UGD and UGER) probably occurred between a protozoan ancestor and mimivirus. While ATCV-1 lacks an epimerase/reductase gene, its Chlorella host may encode this enzyme. Both UGDs and UGER are expressed as late genes, which is consistent with their role in posttranslational modification of capsid proteins. The data in this study provide additional support for the hypothesis that chloroviruses, and maybe mimivirus, encode most, if not all, of the glycosylation machinery involved in the synthesis of specific glycan structures essential for virus replication and infection. PMID:20538863

  16. A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning

    PubMed Central

    Kırlı, Koray; Karaca, Samir; Dehne, Heinz Jürgen; Samwer, Matthias; Pan, Kuan Ting; Lenz, Christof; Urlaub, Henning; Görlich, Dirk

    2015-01-01

    CRM1 is a highly conserved, RanGTPase-driven exportin that carries proteins and RNPs from the nucleus to the cytoplasm. We now explored the cargo-spectrum of CRM1 in depth and identified surprisingly large numbers, namely >700 export substrates from the yeast S. cerevisiae, ≈1000 from Xenopus oocytes and >1050 from human cells. In addition, we quantified the partitioning of ≈5000 unique proteins between nucleus and cytoplasm of Xenopus oocytes. The data suggest new CRM1 functions in spatial control of vesicle coat-assembly, centrosomes, autophagy, peroxisome biogenesis, cytoskeleton, ribosome maturation, translation, mRNA degradation, and more generally in precluding a potentially detrimental action of cytoplasmic pathways within the nuclear interior. There are also numerous new instances where CRM1 appears to act in regulatory circuits. Altogether, our dataset allows unprecedented insights into the nucleocytoplasmic organisation of eukaryotic cells, into the contributions of an exceedingly promiscuous exportin and it provides a new basis for NES prediction. DOI: http://dx.doi.org/10.7554/eLife.11466.001 PMID:26673895

  17. Nucleocytoplasmic protein translocation during mitosis in the social amoebozoan Dictyostelium discoideum.

    PubMed

    O'Day, Danton H; Budniak, Aldona

    2015-02-01

    Mitosis is a fundamental and essential life process. It underlies the duplication and survival of all cells and, as a result, all eukaryotic organisms. Since uncontrolled mitosis is a dreaded component of many cancers, a full understanding of the process is critical. Evolution has led to the existence of three types of mitosis: closed, open, and semi-open. The significance of these different mitotic species, how they can lead to a full understanding of the critical events that underlie the asexual duplication of all cells, and how they may generate new insights into controlling unregulated cell division remains to be determined. The eukaryotic microbe Dictyostelium discoideum has proved to be a valuable biomedical model organism. While it appears to utilize closed mitosis, a review of the literature suggests that it possesses a form of mitosis that lies in the middle between truly open and fully closed mitosis-it utilizes a form of semi-open mitosis. Here, the nucleocytoplasmic translocation patterns of the proteins that have been studied during mitosis in the social amoebozoan D. discoideum are detailed followed by a discussion of how some of them provide support for the hypothesis of semi-open mitosis. PMID:24618050

  18. Phosphorylation-regulated Nucleocytoplasmic Trafficking of Internalized Fibroblast Growth Factor-1

    PubMed Central

    Więdłocha, Antoni; Nilsen, Trine; Wesche, Jørgen; Sørensen, Vigdis; Małecki, Jędrzej; Marcinkowska, Ewa; Olsnes, Sjur

    2005-01-01

    Fibroblast growth factor-1 (FGF-1), which stimulates cell growth, differentiation, and migration, is capable of crossing cellular membranes to reach the cytosol and the nucleus in cells containing specific FGF receptors. The cell entry process can be monitored by phosphorylation of the translocated FGF-1. We present evidence that phosphorylation of FGF-1 occurs in the nucleus by protein kinase C (PKC)δ. The phosphorylated FGF-1 is subsequently exported to the cytosol. A mutant growth factor where serine at the phosphorylation site is exchanged with glutamic acid, to mimic phosphorylated FGF-1, is constitutively transported to the cytosol, whereas a mutant containing alanine at this site remains in the nucleus. The export can be blocked by leptomycin B, indicating active and receptor-mediated nuclear export of FGF-1. Thapsigargin, but not leptomycin B, prevents the appearance of active PKCδ in the nucleus, and FGF-1 is in this case phosphorylated in the cytosol. Leptomycin B increases the amount of phosphorylated FGF-1 in the cells by preventing dephosphorylation of the growth factor, which seems to occur more rapidly in the cytoplasm than in the nucleus. The nucleocytoplasmic trafficking of the phosphorylated growth factor is likely to play a role in the activity of internalized FGF-1. PMID:15574884

  19. A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning.

    PubMed

    Kırlı, Koray; Karaca, Samir; Dehne, Heinz Jürgen; Samwer, Matthias; Pan, Kuan Ting; Lenz, Christof; Urlaub, Henning; Görlich, Dirk

    2015-01-01

    CRM1 is a highly conserved, RanGTPase-driven exportin that carries proteins and RNPs from the nucleus to the cytoplasm. We now explored the cargo-spectrum of CRM1 in depth and identified surprisingly large numbers, namely >700 export substrates from the yeast S. cerevisiae, ≈1000 from Xenopus oocytes and >1050 from human cells. In addition, we quantified the partitioning of ≈5000 unique proteins between nucleus and cytoplasm of Xenopus oocytes. The data suggest new CRM1 functions in spatial control of vesicle coat-assembly, centrosomes, autophagy, peroxisome biogenesis, cytoskeleton, ribosome maturation, translation, mRNA degradation, and more generally in precluding a potentially detrimental action of cytoplasmic pathways within the nuclear interior. There are also numerous new instances where CRM1 appears to act in regulatory circuits. Altogether, our dataset allows unprecedented insights into the nucleocytoplasmic organisation of eukaryotic cells, into the contributions of an exceedingly promiscuous exportin and it provides a new basis for NES prediction. PMID:26673895

  20. The RanGTP Pathway: From Nucleo-Cytoplasmic Transport to Spindle Assembly and Beyond

    PubMed Central

    Cavazza, Tommaso; Vernos, Isabelle

    2016-01-01

    The small GTPase Ran regulates the interaction of transport receptors with a number of cellular cargo proteins. The high affinity binding of the GTP-bound form of Ran to import receptors promotes cargo release, whereas its binding to export receptors stabilizes their interaction with the cargo. This basic mechanism linked to the asymmetric distribution of the two nucleotide-bound forms of Ran between the nucleus and the cytoplasm generates a switch like mechanism controlling nucleo-cytoplasmic transport. Since 1999, we have known that after nuclear envelope breakdown (NEBD) Ran and the above transport receptors also provide a local control over the activity of factors driving spindle assembly and regulating other aspects of cell division. The identification and functional characterization of RanGTP mitotic targets is providing novel insights into mechanisms essential for cell division. Here we review our current knowledge on the RanGTP system and its regulation and we focus on the recent advances made through the characterization of its mitotic targets. We then briefly review the novel functions of the pathway that were recently described. Altogether, the RanGTP system has moonlighting functions exerting a spatial control over protein interactions that drive specific functions depending on the cellular context. PMID:26793706

  1. Signals for Bidirectional Nucleocytoplasmic Transport in the Duck Hepatitis B Virus Capsid Protein

    PubMed Central

    Mabit, Helene; Breiner, Klaus M.; Knaust, Andreas; Zachmann-Brand, Beate; Schaller, Heinz

    2001-01-01

    Hepadnavirus genome replication involves cytoplasmic and nuclear stages, requiring balanced targeting of cytoplasmic nucleocapsids to the nuclear compartment. In this study, we analyze the signals determining capsid compartmentalization in the duck hepatitis B virus (DHBV) animal model, as this system also allows us to study hepadnavirus infection of cultured primary hepatocytes. Using fusions to the green fluorescent protein as a functional assay, we have identified a nuclear localization signal (NLS) that mediates nuclear pore association of the DHBV nucleocapsid and nuclear import of DHBV core protein (DHBc)-derived polypeptides. The DHBc NLS mapped is unique. It bears homology to repetitive NLS elements previously identified near the carboxy terminus of the capsid protein of hepatitis B virus, the human prototype of the hepadnavirus family, but it maps to a more internal position. In further contrast to the hepatitis B virus core protein NLS, the DHBc NLS is not positioned near phosphorylation target sites that are generally assumed to modulate nucleocytoplasmic transport. In functional assays with a knockout mutant, the DHBc NLS was found to be essential for nuclear pore association of the nucleocapsid. The NLS was found to be also essential for virus production from the full-length DHBV genome in transfected cells and from hepatocytes infected with transcomplemented mutant virus. Finally, the DHBc additionally displayed activity indicative of a nuclear export signal, presumably counterbalancing NLS function in the productive state of the infected cell and thereby preventing nucleoplasmic accumulation of nucleocapsids. PMID:11160696

  2. Exploring nucleo-cytoplasmic large DNA viruses in Tara Oceans microbial metagenomes

    PubMed Central

    Hingamp, Pascal; Grimsley, Nigel; Acinas, Silvia G; Clerissi, Camille; Subirana, Lucie; Poulain, Julie; Ferrera, Isabel; Sarmento, Hugo; Villar, Emilie; Lima-Mendez, Gipsi; Faust, Karoline; Sunagawa, Shinichi; Claverie, Jean-Michel; Moreau, Hervé; Desdevises, Yves; Bork, Peer; Raes, Jeroen; de Vargas, Colomban; Karsenti, Eric; Kandels-Lewis, Stefanie; Jaillon, Olivier; Not, Fabrice; Pesant, Stéphane; Wincker, Patrick; Ogata, Hiroyuki

    2013-01-01

    Nucleo-cytoplasmic large DNA viruses (NCLDVs) constitute a group of eukaryotic viruses that can have crucial ecological roles in the sea by accelerating the turnover of their unicellular hosts or by causing diseases in animals. To better characterize the diversity, abundance and biogeography of marine NCLDVs, we analyzed 17 metagenomes derived from microbial samples (0.2–1.6 μm size range) collected during the Tara Oceans Expedition. The sample set includes ecosystems under-represented in previous studies, such as the Arabian Sea oxygen minimum zone (OMZ) and Indian Ocean lagoons. By combining computationally derived relative abundance and direct prokaryote cell counts, the abundance of NCLDVs was found to be in the order of 104–105 genomes ml−1 for the samples from the photic zone and 102–103 genomes ml−1 for the OMZ. The Megaviridae and Phycodnaviridae dominated the NCLDV populations in the metagenomes, although most of the reads classified in these families showed large divergence from known viral genomes. Our taxon co-occurrence analysis revealed a potential association between viruses of the Megaviridae family and eukaryotes related to oomycetes. In support of this predicted association, we identified six cases of lateral gene transfer between Megaviridae and oomycetes. Our results suggest that marine NCLDVs probably outnumber eukaryotic organisms in the photic layer (per given water mass) and that metagenomic sequence analyses promise to shed new light on the biodiversity of marine viruses and their interactions with potential hosts. PMID:23575371

  3. Space Shuttle ascent aborts

    NASA Astrophysics Data System (ADS)

    Schmidgall, Richard A.

    1989-09-01

    Specific guidance functions and trajectory design of return to launch site (RTLS) and transoceanic abort landing (TAL) intact abort profiles, as well as the increasing emphasis on contingency aborts, are presented. Various systems failures including Space Shuttle main engine failures and detailed technical analyses, including the design of powered flight abort trajectories, are considered. The most critical of flight abort situations is the RTLS, while TAL is the preferred abort when uphill capability is no longer available. It is concluded that one principle must remain to ensure continuing success of Space Shuttle flights: namely that intact and contingency aborts necessitate development to ensure safe return of the vehicle, payload, and crew whenever possible.

  4. Nanoelectromechanics of shuttle devices

    NASA Astrophysics Data System (ADS)

    Shekhter, R. I.; Gorelik, L. Y.; Krive, I. V.; Kiselev, M. N.; Parafilo, A. V.; Jonson, M.

    2013-04-01

    A single-electron tunneling (SET) device with a nanoscale central island that can move with respect to the bulk sourceand drain electrodes allows for a nanoelectromechanical (NEM) coupling between the electrical current through the device and the mechanical vibrations of the island. Although the electromechanical "shuttle" instability and the associated phenomenon of single-electron shuttling were predicted more than 15 years ago, both theoretical and experimental studies of NEM-SET structures are still carried out. New functionalities based on quantum coherence, Coulomb correlations and coherent electron-spin dynamics are still of particular interest. In this article we present a short review of recent activities in this area.

  5. Space Shuttle Columbia launch

    NASA Technical Reports Server (NTRS)

    1995-01-01

    A Great Blue Heron seems oblivious to the tremendous spectacle of light and sound generated by a Shuttle liftoff, as the Space Shuttle Columbia (STS-73) soars skyward from Launch Pad 39B. Columbia's seven member crew's mission included continuing experimentation in the Marshall managed payloads including the United States Microgravity Laboratory 2 (USML-2) and the keel-mounted accelerometer that characterizes the very low frequency acceleration environment of the orbiter payload bay during space flight, known as the Orbital Acceleration Research Experiment (OARE).

  6. Stretching the Shuttle

    NASA Astrophysics Data System (ADS)

    Furniss, Tim

    1992-05-01

    A review is presented of the modifications incorporated in the Shuttle Columbia to extend its duration and capabilities in preparation for this extended-duration orbiter (EDO) to fly missions of up to 16 days. Attention is given to the evolution of the program that has changed the Shuttle from a space truck on nominal seven-day sorties to a versatile vehicle that can perform as a space laboratory. Consideration is given to the provision of more electrical power and life support supplies and equipment, the CRYO wafer pallet, advanced general-purpose computers, and an improved radar-altimeter.

  7. Space Shuttle Aging Elastomers

    NASA Technical Reports Server (NTRS)

    Curtis, Cris E.

    2007-01-01

    The reusable Manned Space Shuttle has been flying into Space and returning to earth for more than 25 years. The Space Shuttle's uses various types of elastomers and they play a vital role in mission success. The Orbiter has been in service well past its design life of 10 years or 100 missions. As part of the aging vehicle assessment one question under evaluation is how the elastomers are performing. This paper will outline a strategic assessment plan, how identified problems were resolved and the integration activities between subsystems and Aging Orbiter Working Group.

  8. Shuttle mission plans

    NASA Technical Reports Server (NTRS)

    Visentine, J. T.; Lee, C. M.

    1978-01-01

    Shuttle mission plans recently developed by NASA for the time period 1980-1991 are presented. Standard and optional services, which will be available to users of the Space Transportation System (STS) when it becomes operational in the 1980's, are described. Pricing policies established by NASA to encourage use of the STS by commercial, foreign and other U.S. Government users are explained. The small Self-Contained Payload Program, which will make space flight opportunities available to private citizens and individual experimenters who wish to use the Space Shuttle for investigative research, is discussed.

  9. NASA revises shuttle schedule

    NASA Astrophysics Data System (ADS)

    Wainger, Lisa A.

    The new schedule for Space Shuttle missions and expendable launch vehicles (ELV's) calls for a 7-month delay in sending up the Hubble Space Telescope. NASA was forced to put off launching the telescope until February 1990 to keep the Magellan and Galileo missions within their narrow launch windows. The first post-Challenger shuttle launch is now scheduled for late this month. Discovery's most recent delays were due to a hydrogen leak discovered July 29 that has still not been corrected and an engine valve malfunction during an August 4 test fire.

  10. Nanoparticle shuttle memory

    DOEpatents

    Zettl, Alex Karlwalter

    2012-03-06

    A device for storing data using nanoparticle shuttle memory having a nanotube. The nanotube has a first end and a second end. A first electrode is electrically connected to the first end of the nanotube. A second electrode is electrically connected to the second end of the nanotube. The nanotube has an enclosed nanoparticle shuttle. A switched voltage source is electrically connected to the first electrode and the second electrode, whereby a voltage may be controllably applied across the nanotube. A resistance meter is also connected to the first electrode and the second electrode, whereby the electrical resistance across the nanotube can be determined.

  11. Space Shuttle Flyout: Landing Convoy

    NASA Video Gallery

    A team of trained technicians and specialized trucks and equipment is vital for getting a space shuttle safed after landing, helping the astronauts off the spacecraft and returning the shuttle to i...

  12. EA Shuttle Document Retention Effort

    NASA Technical Reports Server (NTRS)

    Wagner, Howard A.

    2010-01-01

    This slide presentation reviews the effort of code EA at Johnson Space Center (JSC) to identify and acquire databases and documents from the space shuttle program that are adjudged important for retention after the retirement of the space shuttle.

  13. Space Shuttle Era: Main Engines

    NASA Video Gallery

    Producing 500,000 pounds of thrust from a package weighing only 7,500 pounds, the Space Shuttle Main Engines are one of the shining accomplishments of the shuttle program. The success did not come ...

  14. Space shuttles: A pyrotechnic overview

    NASA Technical Reports Server (NTRS)

    Graves, T. J.

    1980-01-01

    Pyrotechnic components specified in Shuttle system designs to accomplish varied tasks during all mission phases are described. The function of these pyrotechnics in the operation of the space shuttle vehicle is discussed. Designs are presented for pyrotechnics with innovative features of those meeting unique shuttle requirements for safety and reliability. A rationale for the qualification and certification of these devices is developed. Maintenance of this qualified system in production hardware is explained through a description of shuttle flight certification review process.

  15. EHD2 shuttles to the nucleus and represses transcription.

    PubMed

    Pekar, Olga; Benjamin, Sigi; Weidberg, Hilla; Smaldone, Silvia; Ramirez, Francesco; Horowitz, Mia

    2012-06-15

    EHD {EH [Eps15 (epidermal growth factor receptor substrate 15) homology]-domain-containing} proteins participate in several endocytic events, such as the internalization and the recycling processes. There are four EHD proteins in mammalian cells, EHD1-EHD4, each with diverse roles in the recycling pathway of endocytosis. EHD2 is a plasma-membrane-associated member of the EHD family that regulates internalization. Since several endocytic proteins have been shown to undergo nucleocytoplasmic shuttling and have been assigned roles in regulation of gene expression, we tested the possibility that EHD proteins also shuttle to the nucleus. Our results showed that, among the three EHD proteins (EHD1-EHD3) that were tested, only EHD2 accumulates in the nucleus under nuclear export inhibition treatment. Moreover, the presence of a NLS (nuclear localization signal) was essential for its entry into the nucleus. Nuclear exit of EHD2 depended partially on its NES (nuclear export signal). Elimination of a potential SUMOylation site in EHD2 resulted in a major accumulation of the protein in the nucleus, indicating the involvement of SUMOylation in the nuclear exit of EHD2. We confirmed the SUMOylation of EHD2 by employing co-immunoprecipitation and the yeast two-hybrid system. Using GAL4-based transactivation assay as well as a KLF7 (Krüppel-like factor 7)-dependent transcription assay of the p21WAF1/Cip1 [CDKN1A (cyclin-dependent kinase inhibitor 1A)] gene, we showed that EHD2 represses transcription. qRT-PCR (quantitative real-time PCR) of RNA from cells overexpressing EHD2 or of RNA from cells knocked down for EHD2 confirmed that EHD2 represses transcription of the p21WAF1/Cip1 gene. PMID:22448906

  16. Shuttle Communications Blackout Study

    NASA Technical Reports Server (NTRS)

    Haben, R. L.; Budica, R. J.

    1983-01-01

    Space Shuttle Orbiter Entry Communications Blackout Study computer program models, investigates, and predicts communication blackout envelopes based on mission entry trajectory and associated data from tracking stations. Of interest to those designing and using communications systems susceptible to blackout. Program is readily adapted to predict entry communications blackout for any nonablative entry vehicle.

  17. The Shuttle Environment Workshop

    NASA Technical Reports Server (NTRS)

    Lehmann, J.; Tanner, S. G. (Editor); Wilkerson, T. (Editor)

    1983-01-01

    Results of shuttle environmental measurement programs were presented. The implications for plasma, infrared and ultraviolet experiments were discussed. The prelaunch environmental conditions, results of key environmental measurements made during the flights of STS 1, 2, 3, 4, and postlanding environmental conditions were covered.

  18. Aboard the Space Shuttle.

    ERIC Educational Resources Information Center

    Steinberg, Florence S.

    This 32-page pamphlet contains color photographs and detailed diagrams which illustrate general descriptive comments about living conditions aboard the space shuttle. Described are details of the launch, the cabin, the condition of weightlessness, food, sleep, exercise, atmosphere, personal hygiene, medicine, going EVA (extra-vehicular activity),…

  19. Chasing the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Mcbride, J. A.; Gray, R. E.

    1981-01-01

    The use and modifications of the T-38 aircraft as chase planes for the STS-1 landing are discussed. Two planes tracked the approach at Edwards AFB, with the lead plane responsible for airspeed and altimetry calibration, technical photography, landing gear status, and height-above-touchdown calls; the second T-38 provided live TV and back-up. Modifications included extension of the landing gear extension speed, increasing the area of the speedbrakes, and installation of a TV system; the goal was to stay with the Shuttle below 40,000 ft while it descended at 15,000 ft/min. Ten T-38's were modified and the deployment of lead and back-up crews during the first Shuttle flight is outlined. Western Test Range C-band radar data of the Orbiter position were transformed into intercept coordinates and VHF relayed to the chase craft. Photographs were taken of the right and left sides and underside of the Shuttle while flying with the speedbrake up to match the Shuttle speed.

  20. Replacing NASA's Shuttle

    NASA Astrophysics Data System (ADS)

    Robertson, Donald F.

    1990-02-01

    The latest NASA Shuttle II proposal for an Advanced Manned Launch System (AMLS) is reviewed. It could achieve total reusability, with a glide-back booster stage and no solid rockets. The propellant load would be divided between the booster and orbiter stages. The AMLS payload of just over nine tons will be limited to crew and 'high-value' cargo, carried in the dorsal pod. Bulky freight and satellites will rely on expendable launchers. AMLS will be a Space Station ferry only and would not be used for on-orbit experiments. The operational history of the Space Shuttle program is shown, as well as its programmed future undertakings. Beyond the proposed Shuttle II, some insight is offered on the conceptual vehicle named Shuttle Z that could be the mainstay of Lunar-Base or Mars expeditions. Needed technologies and key features of a proposed AMLS orbiter are also mentioned. In addition, NASA proposals for a rescue vehicle for Space Station Freedom that will serve to return stranded or injured astronauts to earth is presented. One such proposed crew rescue vehicle would carry four people plus 450 kg of supplies, for a gross mass of 7146 kg.

  1. Shuttle Blast-Off!

    ERIC Educational Resources Information Center

    Gage, Marilyn Kay; And Others

    1993-01-01

    Two articles describe ideas for school library media centers interested in promoting space education. The first article explains how to construct an inexpensive simulation of a space shuttle and suggests associated activities. The second presents steps for identifying resources and organizing them into a resources file; relevant information…

  2. Space Shuttle news reference

    NASA Technical Reports Server (NTRS)

    1981-01-01

    A detailed description of the space shuttle vehicle and associated subsystems is given. Space transportation system propulsion, power generation, environmental control and life support system and avionics are among the topics. Also, orbiter crew accommodations and equipment, mission operations and support, and flight crew complement and crew training are addressed.

  3. Space Shuttle separation mechanisms

    NASA Technical Reports Server (NTRS)

    Rogers, W. F.

    1979-01-01

    The development of space shuttle separation devices is reviewed to illustrate the mechanisms involved in separating the orbiter from the Boeing 747 carrier aircraft and from the externally mounted propellant tank. Other aspects of the separation device development discussed include design evolution, operational experience during the orbiter approach and landing tests, and the work to be accomplished before an operational system becomes a reality.

  4. Space shuttle separation mechanisms

    NASA Technical Reports Server (NTRS)

    Rogers, W. F.

    1978-01-01

    The development of space shuttle separation devices is reviewed to illustrate the mechanisms involved in separating the Orbiter from the Boeing 747 carrier aircraft and from the externally mounted propellant tank. Other aspects of the separation device development discussed include design evolution, operational experience during the orbiter approach and landing tests, and the work required to produce an operational system.

  5. Space shuttle SRM development

    NASA Technical Reports Server (NTRS)

    Adams, I. C.; Call, F. W.

    1979-01-01

    The successful static testing of the fourth Space Shuttle Solid Rocket Motor (SRM) is described. Transportation and support equipment concepts and component reusability are demonstrated. The evolution of the SRM transportation support equipment and special test equipment designs are reviewed, and development activities are discussed. Handling and processing aspects of large, heavy components are reviewed briefly.

  6. Activity and regulation of the centrosome-associated proteasome.

    PubMed

    Fabunmi, R P; Wigley, W C; Thomas, P J; DeMartino, G N

    2000-01-01

    Regulated proteolysis is important for maintaining appropriate cellular levels of many proteins. The bulk of intracellular protein degradation is catalyzed by the proteasome. Recently, the centrosome was identified as a novel site for concentration of the proteasome and associated regulatory proteins (Wigley, W. C., Fabunmi, R. P., Lee, M. G., Marino, C. R., Muallem, S., DeMartino, G. N., and Thomas, P. J. (1999) J. Cell Biol. 145, 481-490). Here we provide evidence that centrosomes contain the active 26 S proteasome that degrades ubiquitinated-protein and proteasome-specific peptide substrates. Moreover, the centrosomes contain an ubiquitin isopeptidase activity. The proteolytic activity is ATP-dependent and is inhibited by proteasome inhibitors. Notably, treatment of cells with inhibitors of proteasome activity promotes redistribution of the proteasome and associated regulatory proteins to the centrosome independent of an intact microtubule system. These data provide biochemical evidence for active proteasomal complexes at the centrosome, highlighting a novel function for this organizing structure. PMID:10617632

  7. Salt Stress and Ethylene Antagonistically Regulate Nucleocytoplasmic Partitioning of COP1 to Control Seed Germination.

    PubMed

    Yu, Yanwen; Wang, Juan; Shi, Hui; Gu, Juntao; Dong, Jingao; Deng, Xing Wang; Huang, Rongfeng

    2016-04-01

    Seed germination, a critical stage initiating the life cycle of a plant, is severely affected by salt stress. However, the underlying mechanism of salt inhibition of seed germination (SSG) is unclear. Here, we report that the Arabidopsis (Arabidopsis thaliana) CONSTITUTIVE PHOTOMORPHOGENESIS1 (COP1) counteracts SSG Genetic assays provide evidence that SSG in loss of function of the COP1 mutant was stronger than this in the wild type. A GUS-COP1 fusion was constitutively localized to the nucleus in radicle cells. Salt treatment caused COP1 to be retained in the cytosol, but the addition of ethylene precursor 1-aminocyclopropane-1-carboxylate had the reverse effect on the translocation of COP1 to the nucleus, revealing that ethylene and salt exert opposite regulatory effects on the localization of COP1 in germinating seeds. However, loss of function of the ETHYLENE INSENSITIVE3 (EIN3) mutant impaired the ethylene-mediated rescue of the salt restriction of COP1 to the nucleus. Further research showed that the interaction between COP1 and LONG HYPOCOTYL5 (HY5) had a role in SSG Correspondingly, SSG in loss of function of HY5 was suppressed. Biochemical detection showed that salt promoted the stabilization of HY5, whereas ethylene restricted its accumulation. Furthermore, salt treatment stimulated and ethylene suppressed transcription of ABA INSENSITIVE5 (ABI5), which was directly transcriptionally regulated by HY5. Together, our results reveal that salt stress and ethylene antagonistically regulate nucleocytoplasmic partitioning of COP1, thereby controlling Arabidopsis seed germination via the COP1-mediated down-regulation of HY5 and ABI5. These findings enhance our understanding of the stress response and have great potential for application in agricultural production. PMID:26850275

  8. Sturgeon nucleo-cytoplasmic large DNA virus phylogeny and PCR tests.

    PubMed

    Clouthier, Sharon C; VanWalleghem, Elissa; Anderson, Eric D

    2015-12-01

    Sturgeon epitheliotropic nucleo-cytoplasmic large DNA viruses (NCLDVs) can cause a lethal disease of the integumentary system. These viruses have not been assigned to any currently recognized family or genus. In this study, phylogenetic analyses using the major capsid protein (MCP) showed that the sturgeon NCLDVs formed a cohesive taxonomic group, could be identified to the species or possibly sub-species level and formed a distinct evolutionary lineage within the Megavirales. The genetic relatedness of the sturgeon virus MCP allowed design of 3 PCR diagnostic tests with analytical specificity (ASp) inclusive of this group of viruses. The conventional PCR test, C1, had broader ASp than the 2 quantitative PCR tests, Q1 and Q2, and was inclusive of the sturgeon viruses as well as some viruses belonging to the families Mimi-, Phycodna-, or Iridoviridae. Q2 had broader specificity than Q1 but both tests recognized the sturgeon NCLDVs and did not cross-react with co-localizing sturgeon herpesviruses. Analytical test performance characteristics evaluated for Q1 and Q2 revealed sensitive assays with observed 50% limits of detection between 3 and 6.25 plasmid copies and high intra- and inter-assay repeatability. Q1 was used to test for sturgeon viruses in endangered populations of lake sturgeon Acipenser fulvescens within the Winnipeg River or Nelson River drainage systems of Manitoba, Canada. Test results indicated that namao virus is endemic in the Nelson River water basin. These tests meet the analytical requirements for diagnostic testing in Canada and are useful tools for disease management in sturgeon conservation stocking programs in North America. PMID:26648102

  9. GGGGCC repeat expansion in C9ORF72 compromises nucleocytoplasmic transport

    PubMed Central

    Freibaum, Brian D.; Lu, Yubing; Lopez-Gonzalez, Rodrigo; Kim, Nam Chul; Almeida, Sandra; Lee, Kyung-Ha; Badders, Nisha; Valentine, Marc; Miller, Bruce L.; Wong, Philip C.; Petrucelli, Leonard; Kim, Hong Joo; Gao, Fen-Biao; Taylor, J. Paul

    2015-01-01

    GGGGCC (G4C2) repeat expansion in a noncoding region of C9ORF72 is the most common cause of sporadic and familial forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD)1,2. The basis for pathogenesis is unknown. To capture the consequences of G4C2 repeat expansion in a tractable genetic system, we generated transgenic fly lines expressing 8, 28 or 58 G4C2 repeat-containing transcripts that do not have a translation start site (AUG) but contain an open-reading frame for green fluorescent protein (GFP) to detect repeat-associated non-AUG (RAN) translation. These transgenic animals show dosage-dependent, repeat length-dependent degeneration in neuronal tissues and RAN translation of dipeptide repeat (DPR) proteins as observed in patients. This model was used in a large-scale, unbiased genetic screen ultimately leading to the identification of 18 genetic modifiers that encode components of the nuclear pore complex (NPC) as well as the machinery that coordinates the export of nuclear RNA and the import of nuclear proteins. Consistent with these results we found morphological abnormalities in the architecture of the nuclear envelope in cells expressing expanded G4C2 repeats in vitro and in vivo. Moreover, we identified a substantial defect in RNA export resulting in retention of RNA in the nuclei of Drosophila cells expressing expanded G4C2 repeats and also in mammalian cells, including aged iPSC-derived neurons from C9ORF72 patients. These studies show that a primary consequence of G4C2 repeat expansion is the compromise of nucleocytoplasmic transport through the nuclear pore, revealing a novel mechanism of neurodegeneration. PMID:26308899

  10. Evolution of the Space Shuttle

    NASA Astrophysics Data System (ADS)

    Nease, Ardell

    1993-02-01

    This paper initially examines the Space Shuttle's past and future role in the exploration and exploitation of space and then discusses the evolution of the Space Shuttle as a cost effective design solution to the nation's and the world's space requirements. The argument for Shuttle evolution is presented and a cost effective approach to evolving the Space Shuttle into tomorrow's Space Transportation System is described. Near term upgrades can increase safety and reliability, avoid obsolescence, reduce operations costs, and increase performance; they can be followed by the long term block changes that incorporate new technologies and make the Space Shuttle dramatically more useful and cost effective to operate. The balance between continued Shuttle System life vs replacement system development and production is placed in the perspective of mission needs, technological leverage, and fiscal reality. The paper concludes that the evolution of the Space Shuttle is the most cost effective solution to the nation's space transportation needs for more than thirty years.

  11. Shuttle freezer conceptual design

    NASA Technical Reports Server (NTRS)

    Proctor, B. W.; Russell, D. J.

    1975-01-01

    A conceptual design for a kit freezer for operation onboard shuttle was developed. The freezer features a self-contained unit which can be mounted in the orbiter crew compartment and is capable of storing food at launch and returning with medical samples. Packaging schemes were investigated to provide the optimum storage capacity with a minimum weight and volume penalty. Several types of refrigeration systems were evaluated to select one which would offer the most efficient performance and lowest hazard of safety to the crew. Detailed performance data on the selected, Stirling cycle principled refrigeration unit were developed to validate the feasibility of its application to this freezer. Thermal analyses were performed to determine the adequacy of the thermal insulation to maintain the desired storage temperature with the design cooling capacity. Stress analyses were made to insure the design structure integrity could be maintained over the shuttle flight regime. A proposed prototype freezer development plan is presented.

  12. Shuttle Net, Tuna Net

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Rockwell International, NASA's prime contractor for the Space Shuttle, asked West Coast Netting (WCN) to develop a safety net for personnel working on the Shuttle Orbiter. This could not be an ordinary net, it had to be relatively small, yet have extraordinary tensile strength. It also had to be fire resistant and resistant to ultraviolet (UV) light. After six months, WCN found the requisite fiber, a polyester-like material called NOMEX. The company was forced to invent a more sophisticated twisting process since conventional methods did not approach specified breaking strength. The resulting product, the Hyperester net, sinks faster and fishes deeper, making it attractive to fishing fleets. A patented treatment for UV protection and greater abrasion resistance make Hyperester nets last longer, and the no-shrink feature is an economic bonus.

  13. New shuttle schedule released

    NASA Astrophysics Data System (ADS)

    1986-10-01

    The Hubble Space Telescope has a tentative launch date of November 17, 1988, according to an announcement made October 3, 1986, by the National Aeronautics and Space Administration (NASA). The plan calls for the first shuttle launch to take place on February 18, 1988, when Discovery is slated to launch a second Tracking and Data Relay Satellite (TDRS). A TDRS satellite was aboard Challenger when it exploded on January 28, 1986.The $1.3 billion telescope would be launched aboard the fifth shuttle mission in 1988. After launching the TDRS satellite, NASA plans to send Atlantis and then Columbia spacebound with military payloads. In September 1988, Discovery would be launched again with a third TDRS satellite.

  14. Space Shuttle flight results

    NASA Technical Reports Server (NTRS)

    Mccarty, B. J.

    1982-01-01

    System function aspects of the first Space Shuttle mission are assessed. Almost 90 mission anomalies have been identified by a mission evaluation team, which were variously attributed to instrumentation sensor failures, improper operation, or design deficiencies. The two most significant problems encountered were Solid Rocket Booster ignition wave overpressure, which exceeded maximum expected pressure by a factor of 2:1 over most of the Orbiter and 5:1 at a sensor location on the aft bulkhead, near the engines, and an inoperative tumble valve, which would normally have imparted to the Space Shuttle External Tank a tumbling motion, after Tank separation, that insures disintegration of the large structure during reentry. The first problem has been solved by strengthening six support struts, and the second by ensuring that the tumble valve is moisture-free.

  15. Shuttle entry guidance revisited

    NASA Technical Reports Server (NTRS)

    Mease, Kenneth D.; Kremer, Jean-Paul

    1992-01-01

    The Shuttle entry guidance concept is reviewed which is aimed at tracking a reference drag trajectory that leads to the specified range and velocity for the initiation of the terminal energy management phase. An approximate method of constructing the domain of attraction is proposed, and its validity is ascertained by simulation. An alternative guidance law yielding global exponential tracking in the absence of control saturation is derived using a feedback linearization method. It is noted that the alternative guidance law does not improve on the stability and performance of the current guidance law, for the operating domain and control capability of the Shuttle. It is suggested that the new guidance law with a larger operating domain and increased lift-to-drag capability would be superior.

  16. Aboard the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Steinberg, F. S.

    1980-01-01

    Livability aboard the space shuttle orbiter makes it possible for men and women scientists and technicians in reasonably good health to join superbly healthy astronauts as space travelers and workers. Features of the flight deck, the mid-deck living quarters, and the subfloor life support and house-keeping equipment are illustrated as well as the provisions for food preparation, eating, sleeping, exercising, and medical care. Operation of the personal hygiene equipment and of the air revitalization system for maintaining sea level atmosphere in space is described. Capabilities of Spacelab, the purpose and use of the remote manipulator arm, and the design of a permanent space operations center assembled on-orbit by shuttle personnel are also depicted.

  17. Space Shuttle Familiarization

    NASA Technical Reports Server (NTRS)

    Mellett, Kevin

    2006-01-01

    This slide presentation visualizes the NASA space center and research facility sites, as well as the geography, launching sites, launching pads, rocket launching, pre-flight activities, and space shuttle ground operations located at NASA Kennedy Space Center. Additionally, highlights the international involvement behind the International Space Station and the space station mobile servicing system. Extraterrestrial landings, surface habitats and habitation systems, outposts, extravehicular activity, and spacecraft rendezvous with the Earth return vehicle are also covered.

  18. Buying a Shuttle ticket

    NASA Technical Reports Server (NTRS)

    Moore, W. F.; Forsythe, C.

    1977-01-01

    A preliminary draft policy for reimbursement for Space Shuttle flights has been developed by NASA in the form of pricing criteria for Space Transportation System (STS) users in domestic and foreign government and industry. The reimbursement policy, the transition from expendable launch vehicles to STS, the new user services, and the interaction of the economics of new user services and STS cost to fly are discussed in the present paper. Current efforts to develop new users are noted.

  19. The Shuttle Enterprise

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The Shuttle Enterprise rolls out of the Palmdale manufacturing facilities with Star Trek television cast members. From left to right they are: Dr. James D. Fletcher, NASA Administrator, DeForest Kelley (Dr. 'Bones' McCoy), George Takei (Mr. Sulu), Nichelle Nichols (Lt. Uhura), Leonard Nimoy (the indefatigable Mr. Spock), Gene Rodenberry (The Great Bird of the Galaxy), and Walter Koenig (Ensign Pavel Checkov).

  20. NASA Space Shuttle Program: Shuttle Environmental Assurance (SEA) Initiative

    NASA Technical Reports Server (NTRS)

    Glover, Steve E.; McCool, Alex (Technical Monitor)

    2002-01-01

    The first Space Shuttle flight was in 1981 and the fleet was originally expected to be replaced with a new generation vehicle in the early 21st century. Space Shuttle Program (SSP) elements proactively address environmental and obsolescence concerns and continue to improve safety and supportability. The SSP manager created the Shuttle Environmental Assurance (SEA) Initiative in 2000. SEA is to provide an integrated approach for the SSP to promote environmental excellence, proactively manage materials obsolescence, and optimize associated resources.

  1. Senescence-related functional nuclear barrier by down-regulation of nucleo-cytoplasmic trafficking gene expression

    SciTech Connect

    Kim, Sung Young; Ryu, Sung Jin; Ahn, Hong Ju; Choi, Hae Ri; Kang, Hyun Tae; Park, Sang Chul

    2010-01-01

    One of the characteristic natures of senescent cells is the hypo- or irresponsiveness not only to growth factors but also to apoptotic stress. In the present study, we confirmed the inhibition of nuclear translocation of activated p-ERK1/2 and NF-kB p50 in response to growth stimuli or LPS in the senescent human diploid fibroblasts. In order to elucidate the underlying mechanism for the senescence-associated hypo-responsiveness, we carried out the comparison study for gene expression profiles through microarray analysis. In consequence, we observed the vast reduction in expression of nucleo-cytoplasmic trafficking genes in senescent cells, when compared with those in young cells. Expression levels of several nucleoporins, karyopherin {alpha}, karyopherin {beta}, Ran, and Ran-regulating factors were confirmed to be down-regulated in senescent HDFs by using RT-PCR and Western blot methods. Taken together, these data suggest the operation of certain senescence-associated functional nuclear barriers by down-regulation of the nucleo-cytoplasmic trafficking genes in the senescent cells.

  2. The high risk HPV16 L2 minor capsid protein has multiple transport signals that mediate its nucleocytoplasmic traffic

    SciTech Connect

    Mamoor, Shahan; Onder, Zeynep; Karanam, Balasubramanyam; Kwak, Kihyuck; Bordeaux, Jennifer; Crosby, Lauren; Roden, Richard B.S.; Moroianu, Junona

    2012-01-20

    In this study we examined the transport signals contributing to HPV16 L2 nucleocytoplasmic traffic using confocal microscopy analysis of enhanced green fluorescent protein-L2 (EGFP-L2) fusions expressed in HeLa cells. We confirmed that both nuclear localization signals (NLSs), the nNLS (1MRHKRSAKRTKR12) and cNLS (456RKRRKR461), previously characterized in vitro (Darshan et al., 2004), function independently in vivo. We discovered that a middle region rich in arginine residues (296SRRTGIRYSRIGNKQTLRTRS316) functions as a nuclear retention sequence (NRS), as mutagenesis of critical arginine residues within this NRS reduced the fraction of L2 in the nucleus despite the presence of both NLSs. Significantly, the infectivity of HPV16 pseudoviruses containing either RR297AA or RR297EE within the L2 NRS was strongly reduced both in HaCaT cells and in a murine challenge model. Experiments using Ratjadone A nuclear export inhibitor and mutation-localization analysis lead to the discovery of a leucine-rich nuclear export signal ({sub 462}LPYFFSDVSL) mediating 16L2 nuclear export. These data indicate that HPV16 L2 nucleocytoplasmic traffic is dependent on multiple functional transport signals.

  3. C9ORF72 poly(GA) aggregates sequester and impair HR23 and nucleocytoplasmic transport proteins.

    PubMed

    Zhang, Yong-Jie; Gendron, Tania F; Grima, Jonathan C; Sasaguri, Hiroki; Jansen-West, Karen; Xu, Ya-Fei; Katzman, Rebecca B; Gass, Jennifer; Murray, Melissa E; Shinohara, Mitsuru; Lin, Wen-Lang; Garrett, Aliesha; Stankowski, Jeannette N; Daughrity, Lillian; Tong, Jimei; Perkerson, Emilie A; Yue, Mei; Chew, Jeannie; Castanedes-Casey, Monica; Kurti, Aishe; Wang, Zizhao S; Liesinger, Amanda M; Baker, Jeremy D; Jiang, Jie; Lagier-Tourenne, Clotilde; Edbauer, Dieter; Cleveland, Don W; Rademakers, Rosa; Boylan, Kevin B; Bu, Guojun; Link, Christopher D; Dickey, Chad A; Rothstein, Jeffrey D; Dickson, Dennis W; Fryer, John D; Petrucelli, Leonard

    2016-05-01

    Neuronal inclusions of poly(GA), a protein unconventionally translated from G4C2 repeat expansions in C9ORF72, are abundant in patients with frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) caused by this mutation. To investigate poly(GA) toxicity, we generated mice that exhibit poly(GA) pathology, neurodegeneration and behavioral abnormalities reminiscent of FTD and ALS. These phenotypes occurred in the absence of TDP-43 pathology and required poly(GA) aggregation. HR23 proteins involved in proteasomal degradation and proteins involved in nucleocytoplasmic transport were sequestered by poly(GA) in these mice. HR23A and HR23B similarly colocalized to poly(GA) inclusions in C9ORF72 expansion carriers. Sequestration was accompanied by an accumulation of ubiquitinated proteins and decreased xeroderma pigmentosum C (XPC) levels in mice, indicative of HR23A and HR23B dysfunction. Restoring HR23B levels attenuated poly(GA) aggregation and rescued poly(GA)-induced toxicity in neuronal cultures. These data demonstrate that sequestration and impairment of nuclear HR23 and nucleocytoplasmic transport proteins is an outcome of, and a contributor to, poly(GA) pathology. PMID:26998601

  4. Space shuttle operational risk assessment

    NASA Astrophysics Data System (ADS)

    Fragola, Joseph R.; Maggio, Gaspare

    1996-03-01

    A Probabilistic Risk Assessment (PRA) of the Space Shuttle system has recently been completed. This year-long effort represents a development resulting from seven years of application of risk technology to the Space Shuttle. These applications were initiated by NASA shortly after the Challenger accident as recommended by the Rogers and Slay Commission reports. The current effort is the first integrated quantitative assessment of the risk of the loss of the shuttle vehicle from 3 seconds prior to liftoff to wheel-stop at mission end. The study which was conducted under the direction of NASA's Shuttle Safety and Mission Assurance office at Johnson Spaceflight Center focused on shuttle operational risk but included consideration of all the shuttle flight and test history since the beginning of the program through Mission 67 in July of 1994.

  5. Nucleocytoplasmic trafficking is essential for BAK1- and BKK1-mediated cell-death control.

    PubMed

    Du, Junbo; Gao, Yang; Zhan, Yanyan; Zhang, Shasha; Wu, Yujun; Xiao, Yao; Zou, Bo; He, Kai; Gou, Xiaoping; Li, Guojing; Lin, Honghui; Li, Jia

    2016-02-01

    BRI1-ASSOCIATED KINASE 1 (BAK1) was initially identified as a co-receptor of the brassinosteroid (BR) receptor BRI1. Genetic analyses also revealed that BAK1 and its closest homolog BAK1-LIKE 1 (BKK1) regulate a BR-independent cell-death control pathway. The double null mutant bak1 bkk1 displays a salicylic acid- and light-dependent cell-death phenotype even without pathogen invasion. Molecular mechanisms of the spontaneous cell death mediated by BAK1 and BKK1 remain unknown. Here we report our identification of a suppressor of bak1 bkk1 (sbb1-1). Genetic analyses indicated that cell-death symptoms in a weak double mutant, bak1-3 bkk1-1, were completely suppressed by the loss-of-function mutation in SBB1, which encodes a nucleoporin (NUP) 85-like protein. Genetic analyses also demonstrated that individually knocking out three other nucleoporin genes from the SBB1-located sub-complex was also able to rescue the cell-death phenotype of bak1-3 bkk1-1. In addition, a DEAD-box RNA helicase, DRH1, was identified in the same protein complex as SBB1 via a proteomic approach. The drh1 mutation also rescues the cell-death symptoms of bak1-3 bkk1-1. Further analyses indicated that export of poly(A)(+) RNA was greatly blocked in the nup and drh1 mutants, resulting in accumulation of significant levels of mRNAs in the nuclei. Over-expression of a bacterial NahG gene to inactivate salicylic acid also rescues the cell-death phenotype of bak1-3 bkk1-1. Mutants suppressing cell-death symptoms always showed greatly reduced salicylic acid contents. These results suggest that nucleocytoplasmic trafficking, especially of molecules directly or indirectly involved in endogenous salicylic acid accumulation, is critical in BAK1- and BKK1-mediated cell-death control. PMID:26775605

  6. Exon recognition and nucleocytoplasmic partitioning determine AMPD1 alternative transcript production.

    PubMed Central

    Mineo, I; Holmes, E W

    1991-01-01

    Two mature transcripts are produced from the rat AMP deaminase 1 (AMPD1) gene, one that retains exon 2 and one from which exon 2 has been removed. The ratio of these two transcripts is controlled by stage-specific and tissue-specific signals (I. Mineo, P. R. H. Clarke, R. L. Sabina, and E. W. Holmes, Mol. Cell. Biol. 10:5271-5278, 1990; R. L. Sabina, N. Ogasawara, and E. W. Holmes, Mol. Cell. Biol. 9:2244-2246, 1989). By using transfection studies with native, mutant, and chimeric minigene constructs, two steps in RNA processing that determine the ratio of these two transcripts have been identified. The first step is recognition of this exon in the primary transcript. The primary transcript is subject to alternative splicing in which exon 2 is either recognized and thereby included in the mature mRNA or is ignored and retained in a composite intron containing intron 1-exon 2-intron 2. The following properties of the primary transcript influence exon recognition. (i) Exon 2 is intrinsically difficult to recognize, possibly because of its small size (only 12 bases) and/or a suboptimal 5' donor site at the exon 2-intron 2 boundary. (ii) Intron 2 plays a permissive role in recognition of exon 2 because it is removed at a relatively slow rate, presumably because of the suboptimal polypyrimidine tract in the putative 3' branch site. The second step in RNA processing that influences the ratio of mature transcripts produced from the AMPD1 gene occurs subsequent to the ligation of exon 2 to exon 1. An RNA intermediate, composed of exon 1-exon 2-intron 2-exon 3, is produced in the first processing step, but it is variably retained in the nucleus. Retention of this intermediate in the nucleus is associated with accumulation of the mature mRNA containing exon 2, while cytoplasmic escape of this intermediate is reactions, exon recognition and nucleocytoplasmic partitioning, determine the relative abundance of alternative mRNAs derived from the AMPD1 gene. Images PMID:1922051

  7. Representative shuttle evaporative heat sink

    NASA Technical Reports Server (NTRS)

    Hixon, C. W.

    1978-01-01

    The design, fabrication, and testing of a representative shuttle evaporative heat sink (RSEHS) system which vaporizes an expendable fluid to provide cooling for the shuttle heat transport fluid loop is reported. The optimized RSEHS minimum weight design meets or exceeds the shuttle flash evaporator system requirements. A cold trap which cryo-pumps flash evaporator exhaust water from the CSD vacuum chamber test facility to prevent water contamination of the chamber pumping equipment is also described.

  8. Space Shuttle operational logistics plan

    NASA Technical Reports Server (NTRS)

    Botts, J. W.

    1983-01-01

    The Kennedy Space Center plan for logistics to support Space Shuttle Operations and to establish the related policies, requirements, and responsibilities are described. The Directorate of Shuttle Management and Operations logistics responsibilities required by the Kennedy Organizational Manual, and the self-sufficiency contracting concept are implemented. The Space Shuttle Program Level 1 and Level 2 logistics policies and requirements applicable to KSC that are presented in HQ NASA and Johnson Space Center directives are also implemented.

  9. Space Shuttle Wireless Crew Communications

    NASA Technical Reports Server (NTRS)

    Armstrong, R. W.; Doe, R. A.

    1982-01-01

    The design, development, and performance characteristics of the Space Shuttle's Wireless Crew Communications System are discussed. This system allows Space Shuttle crews to interface with the onboard audio distribution system without the need for communications umbilicals, and has been designed through the adaptation of commercially available hardware in order to minimize development time. Testing aboard the Space Shuttle Orbiter Columbia has revealed no failures or design deficiencies.

  10. Food packages for Space Shuttle

    NASA Technical Reports Server (NTRS)

    Fohey, M. F.; Sauer, R. L.; Westover, J. B.; Rockafeller, E. F.

    1978-01-01

    The paper reviews food packaging techniques used in space flight missions and describes the system developed for the Space Shuttle. Attention is directed to bite-size food cubes used in Gemini, Gemini rehydratable food packages, Apollo spoon-bowl rehydratable packages, thermostabilized flex pouch for Apollo, tear-top commercial food cans used in Skylab, polyethylene beverage containers, Skylab rehydratable food package, Space Shuttle food package configuration, duck-bill septum rehydration device, and a drinking/dispensing nozzle for Space Shuttle liquids. Constraints and testing of packaging is considered, a comparison of food package materials is presented, and typical Shuttle foods and beverages are listed.

  11. Stennis certifies final shuttle engine

    NASA Technical Reports Server (NTRS)

    2008-01-01

    Steam blasts out of the A-2 Test Stand at Stennis Space Center on Oct. 22 as engineers begin a certification test on engine 2061, the last space shuttle main flight engine scheduled to be built. Since 1975, Stennis has tested every space shuttle main engine used in the program - about 50 engines in all. Those engines have powered more than 120 shuttle missions - and no mission has failed as a result of engine malfunction. For the remainder of 2008 and throughout 2009, Stennis will continue testing of various space shuttle main engine components.

  12. History of Space Shuttle Rendezvous

    NASA Technical Reports Server (NTRS)

    Goodman, John L.

    2011-01-01

    This technical history is intended to provide a technical audience with an introduction to the rendezvous and proximity operations history of the Space Shuttle Program. It details the programmatic constraints and technical challenges encountered during shuttle development in the 1970s and over thirty years of shuttle missions. An overview of rendezvous and proximity operations on many shuttle missions is provided, as well as how some shuttle rendezvous and proximity operations systems and flight techniques evolved to meet new programmatic objectives. This revised edition provides additional information on Mercury, Gemini, Apollo, Skylab, and Apollo/Soyuz. Some chapters on the Space Shuttle have been updated and expanded. Four special focus chapters have been added to provide more detailed information on shuttle rendezvous. A chapter on the STS-39 mission of April/May 1991 describes the most complex deploy/retrieve mission flown by the shuttle. Another chapter focuses on the Hubble Space Telescope servicing missions. A third chapter gives the reader a detailed look at the February 2010 STS-130 mission to the International Space Station. The fourth chapter answers the question why rendezvous was not completely automated on the Gemini, Apollo, and Space Shuttle vehicles.

  13. NASA space shuttle lightweight seat

    NASA Technical Reports Server (NTRS)

    Hansen, Chris; Jermstad, Wayne; Lewis, James; Colangelo, Todd

    1996-01-01

    The Space Shuttle Lightweight Seat-Mission Specialist (LWS-MS) is a crew seat for the mission specialists who fly aboard the Space Shuttle. The LWS-MS is a lightweight replacement for the mission specialist seats currently flown on the Shuttle. Using state-of-the-art analysis techniques, a team of NASA and Lockheed engineers from the Johnson Space Center (JSC) designed a seat that met the most stringent requirements demanded of the new seats by the Shuttle program, and reduced the weight of the seats by 52%.

  14. Shuttle Risk Progression by Flight

    NASA Technical Reports Server (NTRS)

    Hamlin, Teri; Kahn, Joe; Thigpen, Eric; Zhu, Tony; Lo, Yohon

    2011-01-01

    Understanding the early mission risk and progression of risk as a vehicle gains insights through flight is important: . a) To the Shuttle Program to understand the impact of re-designs and operational changes on risk. . b) To new programs to understand reliability growth and first flight risk. . Estimation of Shuttle Risk Progression by flight: . a) Uses Shuttle Probabilistic Risk Assessment (SPRA) and current knowledge to calculate early vehicle risk. . b) Shows impact of major Shuttle upgrades. . c) Can be used to understand first flight risk for new programs.

  15. Space Shuttle Payload Information Source

    NASA Technical Reports Server (NTRS)

    Griswold, Tom

    2000-01-01

    The Space Shuttle Payload Information Source Compact Disk (CD) is a joint NASA and USA project to introduce Space Shuttle capabilities, payload services and accommodations, and the payload integration process. The CD will be given to new payload customers or to organizations outside of NASA considering using the Space Shuttle as a launch vehicle. The information is high-level in a visually attractive format with a voice over. The format is in a presentation style plus 360 degree views, videos, and animation. Hyperlinks are provided to connect to the Internet for updates and more detailed information on how payloads are integrated into the Space Shuttle.

  16. STS-109 Shuttle Mission

    NASA Technical Reports Server (NTRS)

    2001-01-01

    This is the insignia of the STS-109 Space Shuttle mission. Carrying a crew of seven, the Space Shuttle Orbiter Columbia was launched with goals of maintenance and upgrades to the Hubble Space Telescope (HST). The Marshall Space Flight Center had the responsibility for the design, development, and construction of the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. The HST detects objects 25 times fainter than the dimmest objects seen from Earth and provides astronomers with an observable universe 250 times larger than is visible from ground-based telescopes, perhaps as far away as 14 billion light-years. The HST views galaxies, stars, planets, comets, possibly other solar systems, and even unusual phenomena such as quasars, with 10 times the clarity of ground-based telescopes. During the STS-109 mission, the telescope was captured and secured on a work stand in Columbia's payload bay using Columbia's robotic arm where four members of the crew performed five spacewalks completing system upgrades to the HST. Included in those upgrades were: The replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when it original coolant ran out. Lasting 10 days, 22 hours, and 11 minutes, the STS-109 mission was the 27th flight of the Orbiter Columbia and the 108th flight overall in NASA's Space Shuttle Program.

  17. Space Shuttle Cockpit exhibit

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Want to sit in the cockpit of the Space Shuttle and watch astronauts work in outer space? At StenniSphere, you can do that and much more. StenniSphere, the visitor center at John C. Stennis Space Center in Hancock County, Miss., presents 14,000-square-feet of interactive exhibits that depict America's race for space as well as a glimpse of the future. StenniSphere is open free of charge from 9 a.m. to 5 p.m. daily.

  18. Space Shuttle Cockpit

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Want to sit in the cockpit of the Space Shuttle and watch astronauts work in outer space? At StenniSphere, you can do that and much more. StenniSphere, the visitor center at John C. Stennis space Center in Hancock County, Miss., presents 14,000-square-feet of interactive exhibits that depict America's race for space as well as a glimpse of the future. Stennisphere is open free of charge from 9 a.m. to 5 p.m. daily.

  19. Shuttle operational expectations

    NASA Technical Reports Server (NTRS)

    Abrahamson, J. A.

    1982-01-01

    The results of orbital flight tests (OFT) of the Space Shuttle are reviewed, and modifications planned for upcoming operational flights are discussed. The performance of the solid rocket boosters, external tank, main engines, structural system, propulsion system, reaction control system, electric power system, heat rejection system, hydraulic system, avionics, and other systems is described and evaluated as generally highly satisfactory. Payload servicing and deployment were also successfully demonstrated by OFT. Additional facilities planned for the operational flights are briefly described, and improvements that will make the Challenger spacecraft lighter than Columbia, provide it with more thrust, and give it a larger payload are summarized. Some software modifications being introduced are also mentioned.

  20. Space shuttle avionics system

    NASA Technical Reports Server (NTRS)

    Hanaway, John F.; Moorehead, Robert W.

    1989-01-01

    The Space Shuttle avionics system, which was conceived in the early 1970's and became operational in the 1980's represents a significant advancement of avionics system technology in the areas of systems and redundacy management, digital data base technology, flight software, flight control integration, digital fly-by-wire technology, crew display interface, and operational concepts. The origins and the evolution of the system are traced; the requirements, the constraints, and other factors which led to the final configuration are outlined; and the functional operation of the system is described. An overall system block diagram is included.

  1. Space Shuttle Missions Summary

    NASA Technical Reports Server (NTRS)

    Bennett, Floyd V.; Legler, Robert D.

    2011-01-01

    This document has been produced and updated over a 21-year period. It is intended to be a handy reference document, basically one page per flight, and care has been exercised to make it as error-free as possible. This document is basically "as flown" data and has been compiled from many sources including flight logs, flight rules, flight anomaly logs, mod flight descent summary, post flight analysis of mps propellants, FDRD, FRD, SODB, and the MER shuttle flight data and inflight anomaly list. Orbit distance traveled is taken from the PAO mission statistics.

  2. Streamlining shuttle ground operations

    NASA Technical Reports Server (NTRS)

    Heimbold, R. L.; Reichert, W. H.

    1986-01-01

    To meet NASA Space Transportation System goals the Shuttle Processing Contractors have to reduce Space Transportation System ground processing time and ground processing costs. These objectives must be met without compromising safety of flight or safety during assembly, test, and service operations. Ground processing requirements are analyzed to determine critical serial flow paths and costly labor-intensive tasks. Processing improvements are realized by improvements in processing methodology, by application of computer-aided technology, and by modernization of KSC facilities. Ongoing improvement efforts are outlined and progress-to-date is described.

  3. Atmospheric testing of Shuttle

    NASA Technical Reports Server (NTRS)

    Roosa, S. A.

    1974-01-01

    Basic space shuttle launch and orbit configurations are examined. Particular attention is given to the atmospheric portion of reentry and landing, taking into account the pilot techniques, the flight control system, and questions of stability and control. Details are provided regarding a plan which has been proposed for testing the lower Mach number and altitude regime of the entry profile. The ALT program will be primarily concerned with gathering sufficient test data to verify safe subsonic aerodynamic flight and landing in the first orbital flight test approach and landing configuration.

  4. Shuttle autoland support program

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The results of the space shuttle automatic landing support program studies performed from November 1, 1975 to March 31, 1976 were summarized. The following subjects were discussed: (1) software definition review (TAEM pre-final and Autoland only), (2) software definition review (KPIT scheduling), (3) deletion of air data, (4) June 1975 aero data update and check runs, (5) flat turn study, (6) guidance mode switching study, (7) flat turn study-additional data, (8) trajectory shaping study, (9) aero data tolerances, and (10) turbulence model study.

  5. Shuttle Lesson Learned - Toxicology

    NASA Technical Reports Server (NTRS)

    James, John T.

    2010-01-01

    This is a script for a video about toxicology and the space shuttle. The first segment is deals with dust in the space vehicle. The next segment will be about archival samples. Then we'll look at real time on-board analyzers that give us a lot of capability in terms of monitoring for combustion products and the ability to monitor volatile organics on the station. Finally we will look at other issues that are about setting limits and dealing with ground based lessons that pertain to toxicology.

  6. Shuttle Laser Altimeter

    NASA Technical Reports Server (NTRS)

    Bufton, Jack L.; Harding, David J.; Garvin, James B.

    1999-01-01

    The Shuttle Laser Altimeter (SLA) is a Hitchhiker experiment that has flown twice; first on STS-72 in January 1996 and then on STS-85 in August 1997. Both missions produced successful laser altimetry and surface lidar data products from approximately 80 hours per mission of SLA data operations. A total of four Shuttle missions are planned for the SLA series. This paper documents SLA mission results and explains SLA pathfinder accomplishments at the mid-point in this series of Hitchhiker missions. The overall objective of the SLA mission series is the transition of the Goddard Space Flight Center airborne laser altimeter and lidar technology to low Earth orbit as a pathfinder for NASA operational space-based laser remote sensing devices. Future laser altimeter sensors will utilize systems and approaches being tested with SLA, including the Multi-Beam Laser Altimeter (MBLA) and the Geoscience Laser Altimeter System (GLAS). MBLA is the land and vegetation laser sensor for the NASA Earth System Sciences Pathfinder Vegetation Canopy Lidar (VCL) Mission, and GLAS is the Earth Observing System facility instrument on the Ice, Cloud, and Land Elevation Satellite (ICESat). The Mars Orbiting Laser Altimeter, now well into a multi-year mapping mission at the red planet, is also directly benefiting from SLA data analysis methods, just as SLA benefited from MOLA spare parts and instrument technology experience [5] during SLA construction in the early 1990s.

  7. Nucleocytoplasmic shuttling of the rabies virus P protein requires a nuclear localization signal and a CRM1-dependent nuclear export signal

    SciTech Connect

    Pasdeloup, David; Poisson, Nicolas; Raux, Helene; Gaudin, Yves; Ruigrok, Rob W.H. . E-mail: danielle.blondel@vms.cnrs-gif.fr

    2005-04-10

    Rabies virus P protein is a co-factor of the viral RNA polymerase. It has been shown previously that P mRNA directs the synthesis of four N-terminally truncated P products P2, P3, P4, and P5 due to translational initiation by a leaky scanning mechanism at internal Met codons. Whereas P and P2 are located in the cytoplasm, P3, P4, and P5 are found in the nucleus. Here, we have analyzed the molecular basis of the subcellular localization of these proteins. Using deletion mutants fused to GFP protein, we show the presence of a nuclear localization signal (NLS) in the C-terminal part of P (172-297). This domain contains a short lysine-rich stretch ({sup 211}KKYK{sup 214}) located in close proximity with arginine 260 as revealed by the crystal structure of P. We demonstrate the critical role of lysine 214 and arginine 260 in NLS activity. In the presence of Leptomycin B, P is retained in the nucleus indicating that it contains a CRM1-dependent nuclear export signal (NES). The subcellular distribution of P deletion mutants indicates that the domain responsible for export is the amino-terminal part of the protein. The use of fusion proteins that have amino terminal fragments of P fused to {beta}-galactosidase containing the NLS of SV40 T antigen allows us to identify a NES between residues 49 and 58. The localization of NLS and NES determines the cellular distribution of the P gene products.

  8. Methods to assess the nucleocytoplasmic shuttling of the HPV E1 helicase and its effects on cellular proliferation and induction of a DNA damage response.

    PubMed

    Lehoux, Michaël; Fradet-Turcotte, Amélie; Archambault, Jacques

    2015-01-01

    Replication of the human papillomavirus (HPV) double-stranded DNA genome in the nucleus of infected cells relies on the viral proteins E1 and E2 in conjunction with the host DNA replication machinery. This process is tightly linked to the replication of cellular DNA, in part through the cyclin-dependent phosphorylation of E1, which inhibits its export out of the nucleus to promote its accumulation in this compartment during S-phase. It has been recently shown that accumulation of E1 in the nucleus, while a prerequisite for viral DNA replication, leads to the inhibition of cellular proliferation and the activation of a DNA damage response (DDR). Here we describe methods to monitor the subcellular localization of E1 and to assess the deleterious effects of its nuclear accumulation on cellular proliferation, cell cycle progression and the induction of a DDR, using a combination of colony formation assays, immunofluorescence microcopy, and flow cytometry approaches. PMID:25348298

  9. Space Shuttle Glider. Educational Brief.

    ERIC Educational Resources Information Center

    National Aeronautics and Space Administration, Washington, DC.

    Space Shuttle Glider is a scale model of the U.S. Space Shuttle orbiter. The airplane-like orbiter usually remains in Earth orbit for up to two weeks at a time. It normally carries a six- to seven-person crew which includes the mission commander, pilot, and several mission and/or payload specialists who have specialized training associated with…

  10. 1999 Shuttle Small Payloads Symposium

    NASA Technical Reports Server (NTRS)

    Daelemans, Gerard (Editor); Mosier, Frances L. (Editor)

    1999-01-01

    The 1999 Shuttle Small Payloads Symposium is a combined symposia of the Get Away Special (GAS), Space Experiment Module (SEM), and Hitchhiker programs, and is proposed to continue as an annual conference. The focus of this conference is to educate potential Space Shuttle Payload Bay users as to the types of carrier systems provided and for current users to share experiment concepts.

  11. Space Shuttle Program Update

    NASA Technical Reports Server (NTRS)

    2006-01-01

    Bruce Buckingham, from NASA Public Affairs, introduces Wayne Hale, Space Shuttle Program Manager, and Mike Leinbach, NASA launch Director. Wayne Hale begins discussing the Flight Readiness Review (FRR) that has just occurred to see if they were ready to fly. He points out that the review was a debris verification review (DVR). This review was done to ascertain how well they have done to eliminate the potential for debris to come off of the External Tank (ET), or any other part of the launch vehicle. He expresses that they have made significant improvements to the ET. He gives a description of the ET that is presently on the launch pad. Mike Leinbach discusses hardware processing and the condition of the launch vehicle. Questions from the news media about possible modifications to the ice frost ramp, Solid Rocket Booster (SRB) electrical problems, ET foam loss, amount of debris loss expectation during ascent, and return to flight costs are all addressed.

  12. Remodeling and Shuttling

    PubMed Central

    Rodrigueza, Wendi V.; Williams, Kevin Jon; Rothblat, George H.; Phillips, Michael C.

    2016-01-01

    In normal physiology, cells are exposed to cholesterol acceptors of different sizes simultaneously. The current study examined the possible interactions between two different classes of acceptors, one large (large unilamellar phospholipid vesicles, LUVs) and one small (HDL or other small acceptors), added separately or in combination to Fu5AH rat hepatoma cells. During a 24-hour incubation, LUVs of palmitoyl-oleoyl phosphatidylcholine at 1 mg phospholipid (PL) per milliliter extracted ≈20% of cellular unesterified cholesterol (UC) label and mass in a slow, continuous fashion (half-time [t½] for UC efflux was ≈50 hours) and human HDL3 at 25 μg PL per milliliter extracted ≈15% cellular UC label with no change in cellular cholesterol mass (t½ of ≈8 hours). In contrast, the combination of LUVs and HDL3 extracted over 90% of UC label (t½ of ≈4 hours) and ≈50% of the UC mass, indicating synergy. To explain this synergy, specific particle interactions were examined, namely, remodeling, in which the two acceptors alter each other’s composition and thus the ability to mobilize cellular cholesterol, and shuttling, in which the small acceptor ferries cholesterol from cells to the large acceptor. To examine remodeling, LUVs and HDL were coincubated and reisolated before application to cells. This HDL became UC depleted, PL enriched, and lost a small amount of apolipoprotein A-I. Compared with equivalent numbers of control HDL particles, remodeled HDL caused faster efflux (t½ ≈4 hours) and exhibited a greater capacity to sequester cellular cholesterol over 24 hours (≈38% versus ≈15% for control HDL), consistent with their enrichment in PL. Remodeled LUVs still extracted ≈20% of cellular UC. Thus, remodeling accounted for some but not all of the synergy between LUVs and HDL. To examine shuttling, several approaches were used. First, reisolation of particles after an 8-hour exposure to cells revealed that HDL contained very little of the cellular UC

  13. Space Shuttle development update

    NASA Technical Reports Server (NTRS)

    Brand, V.

    1984-01-01

    The development efforts, since the STS-4 flight, in the Space Shuttle (SS) program are presented. The SS improvements introduced in the last two years include lower-weight loads, communication through the Tracking and Data Relay Satellite, expanded extravehicular activity capability, a maneuvering backpack and the manipulator foot restraint, the improvements in thermal projection system, the 'optional terminal area management targeting' guidance software, a rendezvous system with radar and star tracker sensors, and improved on-orbit living conditions. The flight demonstrations include advanced launch techniques (e.g., night launch and direct insertion to orbit); the on-orbit demonstrations; and added entry and launching capabilities. The entry aerodynamic analysis and entry flight control fine tuning are described. Reusability, improved ascent performance, intact abort and landing flexibility, rollout control, and 'smart speedbrakes' are among the many improvements planned for the future.

  14. Shuttle entry guidance

    NASA Technical Reports Server (NTRS)

    Harpold, J. C.; Graves, C. A., Jr.

    1978-01-01

    This paper describes the design of the entry guidance for the Space Shuttle Orbiter. This guidance provides the steering commands for trajectory control from initial penetration of the earth's atmosphere until the terminal area guidance is activated at an earth-relative speed of 2500 fps. At this point, the Orbiter is at a distance of about 50 nmi from the runway threshold, and at an altitude of about 80,000 ft. The entry guidance design is based on an analytic solution of the equations of motion defining the drag acceleration profile that meets the terminal criteria of the entry flight while maintaining the flight within systems and operational constraints. Guidance commands, which are based on a control law that ensures damping of oscillatory type trajectory motion, are computed to steer the Orbiter to this drag acceleration profile.

  15. STS-109 Shuttle Mission

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Carrying a crew of seven, the Space Shuttle Orbiter Columbia soared through some pre-dawn clouds into the sky as it began its 27th flight, STS-109. Launched March 1, 2002, the goal of the mission was the maintenance and upgrade of the Hubble Space Telescope (HST). The Marshall Space Flight Center had the responsibility for the design, development, and construction of the HST, which is the most complex and sensitive optical telescope ever made, to study the cosmos from a low-Earth orbit. The HST detects objects 25 times fainter than the dimmest objects seen from Earth and provides astronomers with an observable universe 250 times larger than is visible from ground-based telescopes, perhaps as far away as 14 billion light-years. The HST views galaxies, stars, planets, comets, possibly other solar systems, and even unusual phenomena such as quasars, with 10 times the clarity of ground-based telescopes. During the STS-109 mission, the telescope was captured and secured on a work stand in Columbia's payload bay using Columbia's robotic arm. Here four members of the crew performed five spacewalks completing system upgrades to the HST. Included in those upgrades were: replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when it original coolant ran out. Lasting 10 days, 22 hours, and 11 minutes, the STS-109 mission was the 108th flight overall in NASA's Space Shuttle Program.

  16. Space Shuttle Strategic Planning Status

    NASA Technical Reports Server (NTRS)

    Norbraten, Gordon L.; Henderson, Edward M.

    2007-01-01

    The Space Shuttle Program is aggressively flying the Space Shuttle manifest for assembling the International Space Station and servicing the Hubble Space Telescope. Completing this flight manifest while concurrently transitioning to the Exploration architecture creates formidable challenges; the most notable of which is retaining critical skills within the Shuttle Program workforce. The Program must define a strategy that will allow safe and efficient fly-out of the Shuttle, while smoothly transitioning Shuttle assets (both human and facility) to support early flight demonstrations required in the development of NASA's Crew Exploration Vehicle (Orion) and Crew and Cargo Launch Vehicles (Ares I). The Program must accomplish all of this while maintaining the current level of resources. Therefore, it will be necessary to initiate major changes in operations and contracting. Overcoming these challenges will be essential for NASA to fly the Shuttle safely, accomplish the Vision for Space Exploration, and ultimately meet the national goal of maintaining a robust space program. This paper will address the Space Shuttle Program s strategy and its current status in meeting these challenges.

  17. Space Shuttle Strategic Planning Status

    NASA Technical Reports Server (NTRS)

    Henderson, Edward M.; Norbraten, Gordon L.

    2006-01-01

    The Space Shuttle Program is aggressively planning the Space Shuttle manifest for assembling the International Space Station and servicing the Hubble Space Telescope. Implementing this flight manifest while concurrently transitioning to the Exploration architecture creates formidable challenges; the most notable of which is retaining critical skills within the Shuttle Program workforce. The Program must define a strategy that will allow safe and efficient fly-out of the Shuttle, while smoothly transitioning Shuttle assets (both human and facility) to support early flight demonstrations required in the development of NASA s Crew Exploration Vehicle (CEV) and Crew and Cargo Launch Vehicles (CLV). The Program must accomplish all of this while maintaining the current level of resources. Therefore, it will be necessary to initiate major changes in operations and contracting. Overcoming these challenges will be essential for NASA to fly the Shuttle safely, accomplish the President s "Vision for Space Exploration," and ultimately meet the national goal of maintaining a robust space program. This paper will address the Space Shuttle Program s strategy and its current status in meeting these challenges.

  18. Pharmacologic considerations for Shuttle astronauts

    NASA Technical Reports Server (NTRS)

    Santy, Patricia A.; Bungo, Michael W.

    1991-01-01

    Medication usage by crewmembers in the preflight and inflight mission periods is common in the Shuttle Program. The most common medical reports for which medication is used are: space motion sickness (SMS), sleeplessness, headache, and backache. A number of medications are available in the Shuttle Medical Kit to treat these problems. Currently, astronauts test all frequently used medications before mission assignment to identify potential side-effects, problems related to performance, personal likes/dislikes, and individual therapeutic effect. However, microgravity-induced changes in drug pharmacokinetics, in combination with multiple operational factors, may significantly alter crewmember responses inflight. This article discusses those factors that may impact pharmacologic efficacy during Shuttle missions.

  19. Shuttle Mockup and Integration Laboratory

    NASA Technical Reports Server (NTRS)

    1984-01-01

    A high-angle view of overall activity in the JSC Shuttle Mockup and Integration Laboratory. In the foreground is the manipulator development facility (MDF), a high fidelity trainer designed to prepare mission specialists for the operation of the remote manipulator system (RMS) on Space Shuttle Orbiters. Here, a helium-filled balloon represents the Long Duration Exposure Facility (LDEF), in the grasp of the RMS end effector. Astronaut crewmembers for STS 41-C mission in the MDF's cabin control the arm while simulating LDEF deployment. Other Shuttle training hardware is visible as well; the full fuselage trainer (FFT) is in upper left and the crew compartment trainer (CCT) is at top center.

  20. Space Shuttle critical function audit

    NASA Technical Reports Server (NTRS)

    Sacks, Ivan J.; Dipol, John; Su, Paul

    1990-01-01

    A large fault-tolerance model of the main propulsion system of the US space shuttle has been developed. This model is being used to identify single components and pairs of components that will cause loss of shuttle critical functions. In addition, this model is the basis for risk quantification of the shuttle. The process used to develop and analyze the model is digraph matrix analysis (DMA). The DMA modeling and analysis process is accessed via a graphics-based computer user interface. This interface provides coupled display of the integrated system schematics, the digraph models, the component database, and the results of the fault tolerance and risk analyses.

  1. Flexible Gates: Dynamic Topologies and Functions for FG Nucleoporins in Nucleocytoplasmic Transport▿ †

    PubMed Central

    Terry, Laura J.; Wente, Susan R.

    2009-01-01

    The nuclear envelope is a physical barrier between the nucleus and cytoplasm and, as such, separates the mechanisms of transcription from translation. This compartmentalization of eukaryotic cells allows spatial regulation of gene expression; however, it also necessitates a mechanism for transport between the nucleus and cytoplasm. Macromolecular trafficking of protein and RNA occurs exclusively through nuclear pore complexes (NPCs), specialized channels spanning the nuclear envelope. A novel family of NPC proteins, the FG-nucleoporins (FG-Nups), coordinates and potentially regulates NPC translocation. The extensive repeats of phenylalanine-glycine (FG) in each FG-Nup directly bind to shuttling transport receptors moving through the NPC. In addition, FG-Nups are essential components of the nuclear permeability barrier. In this review, we discuss the structural features, cellular functions, and evolutionary conservation of the FG-Nups. PMID:19801417

  2. Space Shuttle mission: STS-67

    NASA Technical Reports Server (NTRS)

    1995-01-01

    The Space Shuttle Endeavor, scheduled to launch March 2, 1995 from NASA's Kennedy Space Center, will conduct NASA's longest Shuttle flight prior to date. The mission, designated STS-67, has a number of experiments and payloads, which the crew, commanded by Stephen S. Oswald, will have to oversee. This NASA press kit for the mission contains a general background (general press release, media services information, quick-look facts page, shuttle abort modes, summary timeline, payload and vehicle weights, orbital summary, and crew responsibilities); cargo bay payloads and activities (Astro 2, Get Away Special Experiments); in-cabin payloads (Commercial Minimum Descent Altitude Instrumentation Technology Associates Experiments, protein crystal growth experiments, Middeck Active Control Experiment, and Shuttle Amateur Radio Experiment); and the STS-67 crew biographies. The payloads and experiments are described and summarized to give an overview of the goals, objectives, apparatuses, procedures, sponsoring parties, and the assigned crew members to carry out the tasks.

  3. NASA Now: Shuttle Engineering Challenge

    NASA Video Gallery

    In this installment of NASA Now, you’ll meet Guidance, Navigation and Flight Controls engineer George Hatcher, who talks about the complex system needed to fly the space shuttle at extreme speeds...

  4. Space Shuttle contingency landing operations

    NASA Technical Reports Server (NTRS)

    Allen, Andrew M.; Fleming, Robert D.

    1991-01-01

    The sites and operations involved in emergency landings of the Space Shuttle are discussed. The role of international agreements in developing such sites is addressed. Rescue operations following emergency landings are examined.

  5. Space Shuttle Program Legacy Report

    NASA Technical Reports Server (NTRS)

    Johnson, Scott

    2012-01-01

    Share lessons learned on Space Shuttle Safety and Mission Assurance (S&MA) culture, processes, and products that can guide future enterprises to improve mission success and minimize the risk of catastrophic failures. Present the chronology of the Johnson Space Center (JSC) S&MA organization over the 40-year history of the Space Shuttle Program (SSP) and identify key factors and environments which contributed to positive and negative performance.

  6. Shuttle Fuel Feedliner Cracking Investigation

    NASA Technical Reports Server (NTRS)

    Nesman, Tomas E.; Turner, Jim (Technical Monitor)

    2002-01-01

    This presentation provides an overview of material covered during 'Space Shuttle Fuel Feedliner Cracking Investigation MSFC Fluids Workshop' held November 19-21, 2002. Topics covered include: cracks on fuel feed lines of Orbiter space shuttles, fluid driven cracking analysis, liner structural modes, structural motion in a fluid, fluid borne drivers, three dimensional computational fluid dynamics models, fluid borne drivers from pumps, amplification mechanisms, flow parameter mapping, and flight engine flow map.

  7. Search for ultraviolet Shuttle glow

    NASA Technical Reports Server (NTRS)

    Tennyson, P. D.; Feldman, P. D.; Henry, R. C.

    1987-01-01

    In January 1986, the Space Shuttle Columbia carried two ultraviolet experiments (UVX) in an attempt to observe very weak diffuse emission from various astronomical sources at wavelengths below 3200 A with moderate spectral resolution. The experiment attested to the feasibility of low cost astronomy from the Space Shuttle using Get Away Special canisters. Emissions from O2, O, and NO were detected and shown to be consistent with an atmospheric origin.

  8. Shuttle Hypervelocity Impact Database

    NASA Technical Reports Server (NTRS)

    Hyde, James L.; Christiansen, Eric L.; Lear, Dana M.

    2011-01-01

    With three missions outstanding, the Shuttle Hypervelocity Impact Database has nearly 3000 entries. The data is divided into tables for crew module windows, payload bay door radiators and thermal protection system regions, with window impacts compromising just over half the records. In general, the database provides dimensions of hypervelocity impact damage, a component level location (i.e., window number or radiator panel number) and the orbiter mission when the impact occurred. Additional detail on the type of particle that produced the damage site is provided when sampling data and definitive analysis results are available. Details and insights on the contents of the database including examples of descriptive statistics will be provided. Post flight impact damage inspection and sampling techniques that were employed during the different observation campaigns will also be discussed. Potential enhancements to the database structure and availability of the data for other researchers will be addressed in the Future Work section. A related database of returned surfaces from the International Space Station will also be introduced.

  9. Shuttle Hypervelocity Impact Database

    NASA Technical Reports Server (NTRS)

    Hyde, James I.; Christiansen, Eric I.; Lear, Dana M.

    2011-01-01

    With three flights remaining on the manifest, the shuttle impact hypervelocity database has over 2800 entries. The data is currently divided into tables for crew module windows, payload bay door radiators and thermal protection system regions, with window impacts compromising just over half the records. In general, the database provides dimensions of hypervelocity impact damage, a component level location (i.e., window number or radiator panel number) and the orbiter mission when the impact occurred. Additional detail on the type of particle that produced the damage site is provided when sampling data and definitive analysis results are available. The paper will provide details and insights on the contents of the database including examples of descriptive statistics using the impact data. A discussion of post flight impact damage inspection and sampling techniques that were employed during the different observation campaigns will be presented. Future work to be discussed will be possible enhancements to the database structure and availability of the data for other researchers. A related database of ISS returned surfaces that are under development will also be introduced.

  10. Space shuttle baseline accommodations for payloads

    NASA Technical Reports Server (NTRS)

    1972-01-01

    The space shuttle system as it relates to payloads is described. This study provides potential users of the space shuttle with a uniform base of information on the accommodations between the payload and the shuttle. By utilizing this information, preliminary payload planning and design studies can be evaluated and compared against a common set of shuttle/payload accommodations. This information also minimizes the necessity for each payload study to develop information on the shuttle configuration.

  11. Phosphorylation by casein kinase 1 regulates tonicity-induced osmotic response element-binding protein/tonicity enhancer-binding protein nucleocytoplasmic trafficking.

    PubMed

    Xu, SongXiao; Wong, Catherine C L; Tong, Edith H Y; Chung, Stephen S M; Yates, John R; Yin, YiBing; Ko, Ben C B

    2008-06-20

    The osmotic response element-binding protein (OREBP), also known as tonicity enhancer-binding protein (TonEBP) or NFAT5, is the only known osmo-sensitive transcription factor that mediates cellular adaptations to extracellular hypertonic stress. Although it is well documented that the subcellular localization and transactivation activity of OREBP/TonEBP are tightly regulated by extracellular tonicity, the molecular mechanisms involved remain elusive. Here we show that nucleocytoplasmic trafficking of OREBP/TonEBP is regulated by the dual phosphorylation of Ser-155 and Ser-158. Alanine scanning mutagenesis revealed that Ser-155 is an essential residue that regulates OREBP/TonEBP nucleocytoplasmic trafficking. Tandem mass spectrometry revealed that Ser-155 and Ser-158 of OREBP/TonEBP are both phosphorylated in living cells under hypotonic conditions. In vitro phosphorylation assays further suggest that phosphorylation of the two serine residues proceeds in a hierarchical manner with phosphorylation of Ser-155 priming the phosphorylation of Ser-158 and that these phosphorylations are essential for nucleocytoplasmic trafficking of the transcription factor. Finally, we have shown that the pharmacological inhibition of casein kinase 1 (CK1) abolishes the phosphorylation of Ser-158 and impedes OREBP/TonEBP nuclear export and that recombinant CK1 phosphorylates Ser-158. Knockdown of CK1alpha1L, a novel isoform of CK1, inhibits hypotonicity-induced OREBP/TonEBP nuclear export. Together these data highlight the importance of Ser-155 and Ser-158 in the nucleocytoplasmic trafficking of OREBP/TonEBP and indicate that CK1 plays a major role in regulating this process. PMID:18411282

  12. Phosphorylation by Casein Kinase 1 Regulates Tonicity-induced Osmotic Response Element-binding Protein/Tonicity Enhancer-binding Protein Nucleocytoplasmic Trafficking*

    PubMed Central

    Xu, SongXiao; Wong, Catherine C. L.; Tong, Edith H. Y.; Chung, Stephen S. M.; Yates, John R.; Yin, YiBing; Ko, Ben C. B.

    2008-01-01

    The osmotic response element-binding protein (OREBP), also known as tonicity enhancer-binding protein (TonEBP) or NFAT5, is the only known osmo-sensitive transcription factor that mediates cellular adaptations to extracellular hypertonic stress. Although it is well documented that the subcellular localization and transactivation activity of OREBP/TonEBP are tightly regulated by extracellular tonicity, the molecular mechanisms involved remain elusive. Here we show that nucleocytoplasmic trafficking of OREBP/TonEBP is regulated by the dual phosphorylation of Ser-155 and Ser-158. Alanine scanning mutagenesis revealed that Ser-155 is an essential residue that regulates OREBP/TonEBP nucleocytoplasmic trafficking. Tandem mass spectrometry revealed that Ser-155 and Ser-158 of OREBP/TonEBP are both phosphorylated in living cells under hypotonic conditions. In vitro phosphorylation assays further suggest that phosphorylation of the two serine residues proceeds in a hierarchical manner with phosphorylation of Ser-155 priming the phosphorylation of Ser-158 and that these phosphorylations are essential for nucleocytoplasmic trafficking of the transcription factor. Finally, we have shown that the pharmacological inhibition of casein kinase 1 (CK1) abolishes the phosphorylation of Ser-158 and impedes OREBP/TonEBP nuclear export and that recombinant CK1 phosphorylates Ser-158. Knockdown of CK1α1L, a novel isoform of CK1, inhibits hypotonicity-induced OREBP/TonEBP nuclear export. Together these data highlight the importance of Ser-155 and Ser-158 in the nucleocytoplasmic trafficking of OREBP/TonEBP and indicate that CK1 plays a major role in regulating this process. PMID:18411282

  13. Shuttle Atlantis Landing at Edwards

    NASA Technical Reports Server (NTRS)

    1985-01-01

    NASA's Space Shuttle Atlantis touched down on the lakebed runway at Edwards Air Force Base in California's Mojave Desert Tuesday, 3 December 1985 at 1:33:49 p.m. Pacific Standard Time, concluding the STS 61-B international mission. The eight-day mission successfully deployed three communications satellites including the Mexican Morelos B, the Australian Aussat 2 and an RCA Satcom K-2 satellite. In addition, two spacewalks were performed to experiment with construction of structures in space. Crew of the 61-B mission included Commander Brewster H. Shaw, Jr.; Pilot Bryan D. O'Connor; Mission Specialists Mary L. Cleave, Sherwood C. Spring and Jerry L. Ross; and Payload Specialists Rudolfo Neri Vela of Mexico and Charles Walker of McDonnell Douglas Astronautics Co. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories

  14. Shuttle Discovery Landing at Edwards

    NASA Technical Reports Server (NTRS)

    1989-01-01

    The STS-29 Space Shuttle Discovery mission lands at NASA's then Ames-Dryden Flight Research Facility, Edwards AFB, California, early Saturday morning, 18 March 1989. Touchdown was at 6:35:49 a.m. PST and wheel stop was at 6:36:40 a.m. on runway 22. Controllers chose the concrete runway for the landing in order to make tests of braking and nosewheel steering. The STS-29 mission was very successful, completing the launch of a Tracking and Data Relay communications satellite, as well as a range of scientific experiments. Discovery's five-man crew was led by Commander Michael L. Coats, and included pilot John E. Blaha and mission specialists James P. Bagian, Robert C. Springer, and James F. Buchli. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the laboratories remain inside the payload bay throughout

  15. Shuttle Atlantis Landing at Edwards

    NASA Technical Reports Server (NTRS)

    1988-01-01

    The Space Shuttle Atlantis touches down at 3:35 p.m. PST on 6 December 1988 at NASA's then Ames-Dryden Flight Research Facility at the conclusion of the STS-27 Department of Defense mission. Landing took place on runway 17 of the Rogers Dry Lake, concluding the 4-day, 9-hour, 6-minute mission. The five-man crew was led by Commander Robert L. Gibson and included Pilot Guy S. Gardner; Mission Specialists Jerry L. Ross, William M. Sheperd, and Richard M. Mullane. Atlantis was launched on December 2 from NASA's Kennedy Space Center. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the laboratories remain inside the payload bay throughout the mission. They are then removed after the Space Shuttle returns to Earth and can be reused on future flights. Some of these orbital laboratories, like the Spacelab

  16. Shuttle Atlantis Landing at Edwards

    NASA Technical Reports Server (NTRS)

    1985-01-01

    NASA's Space Shuttle Atlantis touched down on the lakebed runway at Edwards Air Force Base in California's Mojave Desert Tuesday, 3 December 1985 at 1:33:49 p.m. Pacific Standard Time, concluding the STS 61-B international mission. The eight-day mission successfully deployed three communications satellites including the Mexican Morelos B, the Australian Aussat 2 and an RCA Satcom K-2 satellite. In addition, two spacewalks were performed to experiment with construction of structures in space. Crew of the 61-B mission included Commander Brewster H. Shaw, Jr.; Pilot Bryan D. O'Connor; Mission Specialists Mary L. Cleave, Sherwood C. Spring and Jerry L. Ross; and Payload Specialists Rudolfo Neri Vela of Mexico and Charles Walker of McDonnell Douglas Astronautics Co. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories

  17. Shuttle Discovery Landing at Edwards

    NASA Technical Reports Server (NTRS)

    1989-01-01

    The STS-29 Space Shuttle Discovery mission lands at NASA's then Ames-Dryden Flight Research Facility, Edwards AFB, California, early Saturday morning, 18 March 1989. Touchdown was at 6:35:49 a.m. PST and wheel stop was at 6:36:40 a.m. on runway 22. Controllers chose the concrete runway for the landing in order to make tests of braking and nosewheel steering. The STS-29 mission was very successful, completing the launch of a Tracking and Data Relay communications satellite, as well as a range of scientific experiments. Discovery's five-man crew was led by Commander Michael L. Coats, and included pilot John E. Blaha and mission specialists James P. Bagian, Robert C. Springer, and James F. Buchli. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the laboratories remain inside the payload bay throughout

  18. ]Space Shuttle Independent Assessment Team

    NASA Technical Reports Server (NTRS)

    2000-01-01

    The Shuttle program is one of the most complex engineering activities undertaken anywhere in the world at the present time. The Space Shuttle Independent Assessment Team (SIAT) was chartered in September 1999 by NASA to provide an independent review of the Space Shuttle sub-systems and maintenance practices. During the period from October through December 1999, the team led by Dr. McDonald and comprised of NASA, contractor, and DOD experts reviewed NASA practices, Space Shuffle anomalies, as well as civilian and military aerospace experience. In performing the review, much of a very positive nature was observed by the SIAT, not the least of which was the skill and dedication of the workforce. It is in the unfortunate nature of this type of review that the very positive elements are either not mentioned or dwelt upon. This very complex program has undergone a massive change in structure in the last few years with the transition to a slimmed down, contractor-run operation, the Shuttle Flight Operations Contract (SFOC). This has been accomplished with significant cost savings and without a major incident. This report has identified significant problems that must be addressed to maintain an effective program. These problems are described in each of the Issues, Findings or Observations summarized, and unless noted, appear to be systemic in nature and not confined to any one Shuttle sub-system or element. Specifics are given in the body of the report, along with recommendations to improve the present systems.

  19. Calcium-induced cleavage of DNA topoisomerase I involves the cytoplasmic-nuclear shuttling of calpain 2.

    PubMed

    Chou, Shang-Min; Huang, Ting-Hsiang; Chen, Hsiang-Chin; Li, Tsai-Kun

    2011-08-01

    Important to the function of calpains is temporal and spatial regulation of their proteolytic activity. Here, we demonstrate that cytoplasm-resident calpain 2 cleaves human nuclear topoisomerase I (hTOP1) via Ca(2+)-activated proteolysis and nucleoplasmic shuttling of proteases. This proteolysis of hTOP1 was induced by either ionomycin-caused Ca(2+) influx or addition of Ca(2+) in cellular extracts. Ca(2+) failed to induce hTOP1 proteolysis in calpain 2-knockdown cells. Moreover, calpain 2 cleaved hTOP1 in vitro. Furthermore, calpain 2 entered the nucleus upon Ca(2+) influx, and calpastatin interfered with this process. Calpain 2 cleavage sites were mapped at K(158) and K(183) of hTOP1. Calpain 2-truncated hTOP1 exhibited greater relaxation activity but remained able to interact with nucleolin and to form cleavable complexes. Interestingly, calpain 2 appears to be involved in ionomycin-induced protection from camptothecin-induced cytotoxicity. Thus, our data suggest that nucleocytoplasmic shuttling may serve as a novel type of regulation for calpain 2-mediated nuclear proteolysis. PMID:21086148

  20. Space Shuttle Atlantis has liftoff

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Space Shuttle Atlantis lifts off at 6:11:10 a.m. EDT, challenging dawn as it lights up the sky on the fourth attempt to keep a rendezvous with the International Space Station. The mission is taking the crew of seven to the Space Station to deliver logistics and supplies as well as to prepare the Station for the arrival of the Zvezda Service Module, expected to be launched by Russia in July 2000. Also, the crew will conduct one space walk and will reboost the space station from 230 statute miles to 250 statute miles. This will be the third assembly flight to the Space Station. After a 10-day mission, landing is targeted for May 29 at 2:19 a.m. EDT. This is the 98th Shuttle flight and the 21st flight for Shuttle Atlantis.

  1. Space shuttle and life sciences

    NASA Technical Reports Server (NTRS)

    Mason, J. A.

    1977-01-01

    During the 1980's, some 200 Spacelab missions will be flown on space shuttle in earth-orbit. Within these 200 missions, it is planned that at least 20 will be dedicated to life sciences research, projects which are yet to be outlined by the life sciences community. Objectives of the Life Sciences Shuttle/Spacelab Payloads Program are presented. Also discussed are major space life sciences programs including space medicine and physiology, clinical medicine, life support technology, and a variety of space biology topics. The shuttle, spacelab, and other life sciences payload carriers are described. Concepts for carry-on experiment packages, mini-labs, shared and dedicated spacelabs, as well as common operational research equipment (CORE) are reviewed. Current NASA planning and development includes Spacelab Mission Simulations, an Announcement of Planning Opportunity for Life Sciences, and a forthcoming Announcement of Opportunity for Flight Experiments which will together assist in forging a Life Science Program in space.

  2. Liquid lift for the Shuttle

    NASA Astrophysics Data System (ADS)

    Demeis, Richard

    1989-02-01

    After the operational failure of a Solid Rocket Booster (SRB) led to the Space Shuttle Challenger accident, NASA reexamined the use of liquid-fueled units in place of the SRBs in order to ascertain whether they could improve safety and payload. In view of favorable study results obtained, the posibility has arisen of employing a common liquid rocket booster for the Space Shuttle, its cargo version ('Shuttle-C'), and the next-generation Advanced Launch System. The system envisioned would involve two booster units, whose four engines/unit would be fed by integral LOX and kerosene tanks. Mission aborts with one-booster unit and two-unit failures would not be catastrophic, and would respectively allow LEO or an emergency landing in Africa.

  3. Techniques for shuttle trajectory optimization

    NASA Technical Reports Server (NTRS)

    Edge, E. R.; Shieh, C. J.; Powers, W. F.

    1973-01-01

    The application of recently developed function-space Davidon-type techniques to the shuttle ascent trajectory optimization problem is discussed along with an investigation of the recently developed PRAXIS algorithm for parameter optimization. At the outset of this analysis, the major deficiency of the function-space algorithms was their potential storage problems. Since most previous analyses of the methods were with relatively low-dimension problems, no storage problems were encountered. However, in shuttle trajectory optimization, storage is a problem, and this problem was handled efficiently. Topics discussed include: the shuttle ascent model and the development of the particular optimization equations; the function-space algorithms; the operation of the algorithm and typical simulations; variable final-time problem considerations; and a modification of Powell's algorithm.

  4. Subcellular Proteomics Reveals a Role for Nucleo-cytoplasmic Trafficking at the DNA Replication Origin Activation Checkpoint

    PubMed Central

    Mulvey, Claire M.; Tudzarova, Slavica; Crawford, Mark; Williams, Gareth H.; Stoeber, Kai; Godovac-Zimmermann, Jasminka

    2014-01-01

    Depletion of DNA replication initiation factors such as CDC7 kinase triggers the origin activation checkpoint in healthy cells and leads to a protective cell cycle arrest at the G1 phase of the mitotic cell division cycle. This protective mechanism is thought to be defective in cancer cells. To investigate how this checkpoint is activated and maintained in healthy cells, we conducted a quantitative SILAC analysis of the nuclear- and cytoplasmic-enriched compartments of CDC7-depleted fibroblasts and compared them to a total cell lysate preparation. Substantial changes in total abundance and/or subcellular location were detected for 124 proteins, including many essential proteins associated with DNA replication/cell cycle. Similar changes in protein abundance and subcellular distribution were observed for various metabolic processes, including oxidative stress, iron metabolism, protein translation and the tricarboxylic acid cycle. This is accompanied by reduced abundance of two karyopherin proteins, suggestive of reduced nuclear import. We propose that altered nucleo-cytoplasmic trafficking plays a key role in the regulation of cell cycle arrest. The results increase understanding of the mechanisms underlying maintenance of the DNA replication origin activation checkpoint and are consistent with our proposal that cell cycle arrest is an actively maintained process that appears to be distributed over various subcellular locations. PMID:23320540

  5. The effect of light color on the nucleocytoplasmic and chloroplast cycle of the green chlorococcal alga Scenedesmus obliquus.

    PubMed

    Cepák, V; Pribyl, P; Vítová, M

    2006-01-01

    The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, the light phase of the cell cycle (time when first autospores started to be released) was prolonged. The length of dark phase (time when 100 % of daughters were allowed to release from mothers) was not influenced and was the same for all colors. Critical cell size for cell division in green light was shifted to a smaller size (compared with cells grown in other lights) and so was the size of released daughters. The nuclear cycle was slowed in blue and even in green light, contrary to cells grown in red and white light. At the beginning of the cell cycle, one-nucleus daughters possess approximately 10 nucleoids; during the cell cycle their number doubled in all variants before the division of nuclei. Both events were delayed in cultures grown more slowly most markedly in green light. Smaller daughters in the green variant possessed a lower number of nucleoids. Motile cells released in continuous green or blue lights but not in red one were rarely observed. PMID:17007440

  6. shRNA library screening identifies nucleocytoplasmic transport as a mediator of BCR-ABL1 kinase-independent resistance.

    PubMed

    Khorashad, Jamshid S; Eiring, Anna M; Mason, Clinton C; Gantz, Kevin C; Bowler, Amber D; Redwine, Hannah M; Yu, Fan; Kraft, Ira L; Pomicter, Anthony D; Reynolds, Kimberly R; Iovino, Anthony J; Zabriskie, Matthew S; Heaton, William L; Tantravahi, Srinivas K; Kauffman, Michael; Shacham, Sharon; Chenchik, Alex; Bonneau, Kyle; Ullman, Katharine S; O'Hare, Thomas; Deininger, Michael W

    2015-03-12

    The mechanisms underlying tyrosine kinase inhibitor (TKI) resistance in chronic myeloid leukemia (CML) patients lacking explanatory BCR-ABL1 kinase domain mutations are incompletely understood. To identify mechanisms of TKI resistance that are independent of BCR-ABL1 kinase activity, we introduced a lentiviral short hairpin RNA (shRNA) library targeting ∼5000 cell signaling genes into K562(R), a CML cell line with BCR-ABL1 kinase-independent TKI resistance expressing exclusively native BCR-ABL1. A customized algorithm identified genes whose shRNA-mediated knockdown markedly impaired growth of K562(R) cells compared with TKI-sensitive controls. Among the top candidates were 2 components of the nucleocytoplasmic transport complex, RAN and XPO1 (CRM1). shRNA-mediated RAN inhibition or treatment of cells with the XPO1 inhibitor, KPT-330 (Selinexor), increased the imatinib sensitivity of CML cell lines with kinase-independent TKI resistance. Inhibition of either RAN or XPO1 impaired colony formation of CD34(+) cells from newly diagnosed and TKI-resistant CML patients in the presence of imatinib, without effects on CD34(+) cells from normal cord blood or from a patient harboring the BCR-ABL1(T315I) mutant. These data implicate RAN in BCR-ABL1 kinase-independent imatinib resistance and show that shRNA library screens are useful to identify alternative pathways critical to drug resistance in CML. PMID:25573989

  7. Nucleocytoplasmic human O-GlcNAc transferase is sufficient for O-GlcNAcylation of mitochondrial proteins

    PubMed Central

    Trapannone, Riccardo; Mariappa, Daniel; Ferenbach, Andrew T.; vanAalten, Daan M.F.

    2016-01-01

    O-linked N-acetylglucosamine modification (O-GlcNAcylation) is a nutrient-dependent protein post-translational modification (PTM), dynamically and reversibly driven by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) that catalyse the addition and the removal of the O-GlcNAc moieties to/from serine and threonine residues of target proteins respectively. Increasing evidence suggests involvement of O-GlcNAcylation in many biological processes, including transcription, signalling, neuronal development and mitochondrial function. The presence of a mitochondrial O-GlcNAc proteome and a mitochondrial OGT (mOGT) isoform has been reported. We explored the presence of mOGT in human cell lines and mouse tissues. Surprisingly, analysis of genomic sequences indicates that this isoform cannot be expressed in most of the species analysed, except some primates. In addition, we were not able to detect endogenous mOGT in a range of human cell lines. Knockdown experiments and Western blot analysis of all the predicted OGT isoforms suggested the expression of only a single OGT isoform. In agreement with this, we demonstrate that overexpression of the nucleocytoplasmic OGT (ncOGT) isoform leads to increased O-GlcNAcylation of mitochondrial proteins, suggesting that ncOGT is necessary and sufficient for the generation of the O-GlcNAc mitochondrial proteome. PMID:27048592

  8. Nucleocytoplasmic human O-GlcNAc transferase is sufficient for O-GlcNAcylation of mitochondrial proteins.

    PubMed

    Trapannone, Riccardo; Mariappa, Daniel; Ferenbach, Andrew T; van Aalten, Daan M F

    2016-06-15

    O-linked N-acetylglucosamine modification (O-GlcNAcylation) is a nutrient-dependent protein post-translational modification (PTM), dynamically and reversibly driven by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) that catalyse the addition and the removal of the O-GlcNAc moieties to/from serine and threonine residues of target proteins respectively. Increasing evidence suggests involvement of O-GlcNAcylation in many biological processes, including transcription, signalling, neuronal development and mitochondrial function. The presence of a mitochondrial O-GlcNAc proteome and a mitochondrial OGT (mOGT) isoform has been reported. We explored the presence of mOGT in human cell lines and mouse tissues. Surprisingly, analysis of genomic sequences indicates that this isoform cannot be expressed in most of the species analysed, except some primates. In addition, we were not able to detect endogenous mOGT in a range of human cell lines. Knockdown experiments and Western blot analysis of all the predicted OGT isoforms suggested the expression of only a single OGT isoform. In agreement with this, we demonstrate that overexpression of the nucleocytoplasmic OGT (ncOGT) isoform leads to increased O-GlcNAcylation of mitochondrial proteins, suggesting that ncOGT is necessary and sufficient for the generation of the O-GlcNAc mitochondrial proteome. PMID:27048592

  9. STS-63 Space Shuttle report

    NASA Astrophysics Data System (ADS)

    Fricke, Robert W., Jr.

    1995-06-01

    The STS-63 Space Shuttle Program Mission Report summarizes the Payload activities and provides detailed data on the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle Main Engine (SSME) systems performance during this sixty-seventh flight of the Space Shuttle Program, the forty-second since the return to flight, and twentieth flight of the Orbiter vehicle Discovery (OV-103). In addition to the OV-103 Orbiter vehicle, the flight vehicle consisted of an ET that was designated ET-68; three SSME's that were designated 2035, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's that were designated BI-070. The RSRM's that were an integral part of the SRB's were designated 360Q042A for the left SRB and 360L042B for the right SRB. The STS-63 mission was planned as an 8-day duration mission with two contingency days available for weather avoidance or Orbiter contingency operations. The primary objectives of the STS-63 mission were to perform the Mir rendezvous operations, accomplish the Spacehab-3 experiments, and deploy and retrieve the Shuttle Pointed Autonomous Research Tool for Astronomy-204 (SPARTAN-204) payload. The secondary objectives were to perform the Cryogenic Systems Experiment (CSE)/Shuttle Glo-2 Experiment (GLO-2) Payload (CGP)/Orbital Debris Radar Calibration Spheres (ODERACS-2) (CGP/ODERACS-2) payload objectives, the Solid Surface Combustion Experiment (SSCE), and the Air Force Maui Optical Site Calibration Tests (AMOS). The objectives of the Mir rendezvous/flyby were to verify flight techniques, communication and navigation-aid sensor interfaces, and engineering analyses associated with Shuttle/Mir proximity operations in preparation for the STS-71 docking mission.

  10. STS-63 Space Shuttle report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1995-01-01

    The STS-63 Space Shuttle Program Mission Report summarizes the Payload activities and provides detailed data on the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle Main Engine (SSME) systems performance during this sixty-seventh flight of the Space Shuttle Program, the forty-second since the return to flight, and twentieth flight of the Orbiter vehicle Discovery (OV-103). In addition to the OV-103 Orbiter vehicle, the flight vehicle consisted of an ET that was designated ET-68; three SSME's that were designated 2035, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's that were designated BI-070. The RSRM's that were an integral part of the SRB's were designated 360Q042A for the left SRB and 360L042B for the right SRB. The STS-63 mission was planned as an 8-day duration mission with two contingency days available for weather avoidance or Orbiter contingency operations. The primary objectives of the STS-63 mission were to perform the Mir rendezvous operations, accomplish the Spacehab-3 experiments, and deploy and retrieve the Shuttle Pointed Autonomous Research Tool for Astronomy-204 (SPARTAN-204) payload. The secondary objectives were to perform the Cryogenic Systems Experiment (CSE)/Shuttle Glo-2 Experiment (GLO-2) Payload (CGP)/Orbital Debris Radar Calibration Spheres (ODERACS-2) (CGP/ODERACS-2) payload objectives, the Solid Surface Combustion Experiment (SSCE), and the Air Force Maui Optical Site Calibration Tests (AMOS). The objectives of the Mir rendezvous/flyby were to verify flight techniques, communication and navigation-aid sensor interfaces, and engineering analyses associated with Shuttle/Mir proximity operations in preparation for the STS-71 docking mission.

  11. Space Shuttle booster recovery planning.

    NASA Technical Reports Server (NTRS)

    Godfrey, R. E.

    1973-01-01

    At the initiation of the Space Shuttle Program, recoverable solid rocket boosters were base-lined, with an estimated savings of 30 per cent over expendable solid rockets. Present studies indicate that the solid rocket boosters in the 142-inch diameter range can be recovered using state-of-the-art recovery systems. Marshall Space Flight Center is conducting extensive studies to establish the most cost effective recovery system for the present Shuttle boosters. Model drop testing, in various facilities, and structural load testing are being conducted with model sizes ranging from 6 inches to 120 inches in diameter.

  12. Shuttle Propulsion Overview to NATO

    NASA Technical Reports Server (NTRS)

    Lightfoot, Robert

    2006-01-01

    In the early morning on Saturday, February 1, 2003, the Space Shuttle Columbia broke up during entry. After extensive investigation of the accident and recommendations made by the Columbia Accident Investigation Board, President Bush gave the vision for space exploration for NASA, which include return the Space Shuttle to flight as soon as practical, complete assembly of the ISS by the end of the decade, initiate robotic missions to the moon no later than 2008, develop a new Crew Exploration Vehicle, conduct first robotic, then human missions to Mars and extend human exploration across the solar system.

  13. Mechanistic investigations of shuttle glow

    NASA Technical Reports Server (NTRS)

    Caledonia, G. E.; Holtzclaw, K. W.; Krech, R. H.; Sonnenfroh, D. M.; Leone, A.; Blumberg, W. A. M.

    1993-01-01

    A series of laboratory measurements have been performed in order to provide a mechanistic interpretation for the visible shuttle glow. These studies involved interactions of an 8 km/s oxygen atom beam with both contaminant dosed surfaces and gaseous targets. We conclude that visible shuttle glow arises from surface mediated O + NO recombination via a Langmuir-Hinshelwood mechanism and that the gas-phase exchange reaction O + N2 - NO + N provides a viable source of precursor NO above surfaces oriented in the ram direction.

  14. Space Shuttle Main Engine. Overview

    NASA Astrophysics Data System (ADS)

    Jackson, Eugene D.

    An overview of the Space Shuttle Main Engine (SSME) is presented. The Space Shuttle propulsion system consists of two large solid booster motors, three SSME's, two orbital maneuvering system engines, and 44 reaction control system thrusters. The three SSME's burn liquid hydrogen and liquid oxygen from the external tank and are sequentially started at launch. Engine thrust is throttleable. The major components and some of their key features and operational parameters are outlined. The life and reliability being achieved by the SSME are presented.

  15. Improving the breed - Shuttle development

    NASA Technical Reports Server (NTRS)

    Brand, V.

    1985-01-01

    An evaluation is made of design improvements that have been made to the Space Shuttle System, and the performance gains obtained; the most important of these stem from efforts to refine procedures for rendezvous with stricken satellites, in order to repair them. Ascent performance has been improved through Space Shuttle Main Engine thrust improvements and external tank weight reductions. On-orbit living convenience has been enhanced by the addition of small sleeping compartments and a galley. Greater flexibility has been obtained for reentry and landing maneuvers. Attention is given to problems which continue to be posed by the thermal protection tiles.

  16. Selected optimal shuttle entry computations

    NASA Technical Reports Server (NTRS)

    Sullivan, H. C.

    1974-01-01

    Parameterization and the Davidon-Fletcher-Powell method are used to study the characteristics of optimal shuttle entry trajectories. Two problems of thermal protective system weight minimization are considered: roll modulation and roll plus an angle-of-attack modulation. Both problems are targeted for the edges of the entry footprint. Results consistent with constraints on loads and control bounds are particularly well-behaved and strongly support 'energy' approximation results obtained for the case of symmetric flight by Kelley and Sullivan (1973). Furthermore, results indicate that optimal shuttle entry trajectories should be easy to duplicate and to analyze by using simple techniques.

  17. Space Shuttle orbiter separation bolts

    NASA Technical Reports Server (NTRS)

    Ritchie, R. S.

    1979-01-01

    Evolution of the space shuttle from previous spacecraft systems dictated growth and innovative design of previously standard ordnance devices. Initially, one bolt design was programmed for both 747 and external tank application. However, during development and subsequent analyses, two distinct designs evolved. The unique requirements of both bolts include: high combined loading, redundant initiation, flush separation plane, self-righting and shank attenuation. Of particular interest are the test methods, problem areas, and use of subscale models which demonstrated feasibility at an early phase in the program. The techniques incorporated in the shuttle orbiter bolts are applicable to other mechanisms.

  18. The Space Shuttle in perspective

    NASA Technical Reports Server (NTRS)

    Hosenball, S. N.

    1981-01-01

    Commercial aspects of the Space Shuttle are examined, with attention given to charges to users, schedule of launches and reimbursement, kinds of payload and their selection, NASA authority, space allocation, and risk, liability, and insurance. It is concluded that insurance to reduce the risk, incentives that NASA is willing to make available to U.S. industry, and the demonstrated willingness of industry and the financial community to invest their funds in space ventures indicate that the new Shuttle capabilities will exponentially increase commercial activities in space during the 1980s.

  19. Mechanistic investigations of shuttle glow

    SciTech Connect

    Caledonia, G.E.; Holtzclaw, K.W.; Krech, R.H.; Sonnenfroh, D.M. ); Leone, A. ); Blumber, W.A.M. )

    1993-03-01

    A series of laboratory measurements have been performed in order to provide a mechanistic interpretation for the visible shuttle glow. These studies involved interactions in an 8 km/s oxygen atom beam with both contaminant dosed surfaces and gaseous targets. The authors conclude that visible shuttle glow arises from surface mediated O + NO recombination via a Langmuir-Hinshelwood mechanism and that the gas-phase exchange reaction O + N[sub 2] [r arrow] NO + N provides a viable source of precursor NO above surfaces oriented in the ram direction. 35 refs., 4 figs.

  20. Airbreathing engines for space shuttle

    NASA Technical Reports Server (NTRS)

    Glassman, A. J.; Stewart, W. L.; Nosek, S. M.

    1972-01-01

    The requirements imposed on the airbreathing engines by the shuttle mission and some results from engine design studies are discussed. In particular, some of the engine system weight study results are presented, potential problem areas and required engine modifications are identified, and testing requirements for a development and qualification program are discussed. The engines of interest for the shuttle are engines that are currently being developed for other applicatons. The potential problems, engine modifications, and testing requirements result primarily from the new environments associated with launch, space residence, and reentry.

  1. Issues in Shuttle System Instrumentation

    NASA Technical Reports Server (NTRS)

    James, George

    2004-01-01

    The purose: a) Customer's perspective on Space Shuttle Return to Flight instrumentation; b) Focus on the difficult instrumentation issues; and c) Enable a discussion of new technologies (i.e.- NANO/MEMS/Small Tech) that could enhance Shuttle instrumentation posture. The T-10 Umbilical allows the vehicle instruments to be monitored and recorded prior to each launch and retract during launch.Launch Complex Instrumentation are instruments needed for assessment of Launch Commit Criteria (LCC) Salt-air and launch environments are issues. Instrumentation (Drag-On Instrumentation) can be added as needed to the vehicle for non-flight use. The current Roll-out Fatigue Testing is a primary example.

  2. Continual Improvement in Shuttle Logistics

    NASA Technical Reports Server (NTRS)

    Flowers, Jean; Schafer, Loraine

    1995-01-01

    It has been said that Continual Improvement (CI) is difficult to apply to service oriented functions, especially in a government agency such as NASA. However, a constrained budget and increasing requirements are a way of life at NASA Kennedy Space Center (KSC), making it a natural environment for the application of CI tools and techniques. This paper describes how KSC, and specifically the Space Shuttle Logistics Project, a key contributor to KSC's mission, has embraced the CI management approach as a means of achieving its strategic goals and objectives. An overview of how the KSC Space Shuttle Logistics Project has structured its CI effort and examples of some of the initiatives are provided.

  3. Shuttle extravehicular life support equipment

    NASA Technical Reports Server (NTRS)

    Sutton, J. G.

    1973-01-01

    A Shuttle EVA/IVA Requirements Study was conducted by Hamilton Standard for NASA. The objectives of this study were to establish a baseline EVA approach for Shuttle and to prepare requirements for the EVA equipment required to support these operations. This paper presents the results of the EVA life support requirements definition effort and defines candidate configurations which meet these requirements. Various subsystem and system concepts were identified and evaluated to determine the most desirable approaches. Both independent and umbilical configurations are considered. Because certain EVA missions could involve contamination-sensitive payloads, the impact of integrating noncontaminating equipment is also considered.

  4. Shuttle Endeavour Flyover of Los Angeles Landmarks

    NASA Video Gallery

    Space shuttle Endeavour atop NASA's Shuttle Carrier Aircraft flew over many Los Angeles area landmarks on its final ferry flight Sept. 21, 2012, including the Coliseum, the Hollywood Sign, Griffith...

  5. Shuttle Performance: Lessons Learned, Part 2

    NASA Technical Reports Server (NTRS)

    Arrington, J. P. (Compiler); Jones, J. J. (Compiler)

    1983-01-01

    Several areas of Space Shuttle technology were addressed including aerothermal environment, thermal protection, measurement and analysis, Shuttle carrier aerodynamics, entry analysis of the STS-3, and an overview of each section.

  6. Space Operations Center: Shuttle interaction study

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The implication of using the Shuttle with the Space Operation Center (SOC), including constraints that the Shuttle will place upon the SOC design. The study identifies the considerations involved in the use of the Shuttle as a part of the SOC concept, and also identifies the constraints to the SOC imposed by the Shuttle in its interactions with the SOC, and on the design or technical solutions which allow satisfactory accomplishment of the interactions.

  7. Space Shuttle Projects Overview to Columbia Air Forces War College

    NASA Technical Reports Server (NTRS)

    Singer, Jody; McCool, Alex (Technical Monitor)

    2000-01-01

    This paper presents, in viewgraph form, a general overview of space shuttle projects. Some of the topics include: 1) Space Shuttle Projects; 2) Marshall Space Flight Center Space Shuttle Projects Office; 3) Space Shuttle Propulsion systems; 4) Space Shuttle Program Major Sites; 5) NASA Office of Space flight (OSF) Center Roles in Space Shuttle Program; 6) Space Shuttle Hardware Flow; and 7) Shuttle Flights To Date.

  8. Transition to the space shuttle operations era

    NASA Technical Reports Server (NTRS)

    1985-01-01

    The tasks involved in the Space Shuttle Development Program are discussed. The ten major characteristics of an operational Shuttle are described, as well as the changes occurring in Shuttle processing, on-line operations, operations engineering, and support operations. A summary is given of tasks and goals that are being pursued in the effort to create a cost effective and efficient system.

  9. Shuttle Global Positioning (GPS) System design study

    NASA Technical Reports Server (NTRS)

    Nilsen, P.; Huth, G. K.

    1980-01-01

    Investigations of certain aspects and problems of the shuttle global positioning system GPS, are presented. Included are: test philosophy and test outline; development of a phase slope specification for the shuttle GPS antenna; an investigation of the shuttle jamming vulnerability; and an expression for the GPS signal to noise density ratio for the thermal protection system.

  10. A decade on board America's Space Shuttle

    NASA Technical Reports Server (NTRS)

    1991-01-01

    Spectacular moments from a decade (1981-1991) of Space Shuttle missions, captured on film by the astronauts who flew the missions, are presented. First hand accounts of astronauts' experiences aboard the Shuttle are given. A Space Shuttle mission chronology featuring flight number, vehicle name, crew, launch and landing dates, and mission highlights is given in tabular form.

  11. Shuttle Global Positioning System (GPS) design study

    NASA Technical Reports Server (NTRS)

    Nilsen, P. W.

    1979-01-01

    The effects of oscillator noise on Shuttle Global Positioning System (GPS) receiver performance, GPS navigation system self-test, GPS ground transmitter design to augment shuttle navigation, the effect of ionospheric delay modelling on GPS receiver design, and GPS receiver tracking of Shuttle transient maneuvers were investigated.

  12. Earth Resources Survey and the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Stow, W. K.; Andryczyk, R. W.

    1975-01-01

    The impact that the shuttle is expected to have on the Earth Resources Program and several concepts for exploiting the shuttle characteristics are discussed. The utilization of the space shuttle in its sortie mode for earth resources and the application of an earth observations standard package to earth resources missions were studied.

  13. Space shuttle operations integration plan

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The Operations Integration Plan is presented, which is to provide functional definition of the activities necessary to develop and integrate shuttle operating plans and facilities to support flight, flight control, and operations. It identifies the major tasks, the organizations responsible, their interrelationships, the sequence of activities and interfaces, and the resultant products related to operations integration.

  14. The Space Shuttle At Work.

    ERIC Educational Resources Information Center

    Allaway, Howard

    This report describes the Space Shuttle vehicles and is prepared by the Scientific and Technical Information Branch and Division of Public Affairs of the National Aeronautics and Space Administration. The book is divided into nine chapters including information about the launching, flight, and orbit of the ships; the satellites and previous space…

  15. Living aboard the Space Shuttle

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The crew habitat of the Space Shuttle is briefly characterized. Subjects discussed include the overall layout of the crew quarters; the air-purification and climate-control facilities; menus and food-preparation techniques; dishwashing, laundry, toilet, bathing, and shaving procedures; and recreation and sleeping accommodations. Drawings and a photograph are provided.

  16. Space Shuttle Propulsion System Reliability

    NASA Technical Reports Server (NTRS)

    Welzyn, Ken; VanHooser, Katherine; Moore, Dennis; Wood, David

    2011-01-01

    This session includes the following sessions: (1) External Tank (ET) System Reliability and Lessons, (2) Space Shuttle Main Engine (SSME), Reliability Validated by a Million Seconds of Testing, (3) Reusable Solid Rocket Motor (RSRM) Reliability via Process Control, and (4) Solid Rocket Booster (SRB) Reliability via Acceptance and Testing.

  17. Space Shuttle Launch: STS-129

    NASA Video Gallery

    STS-129. Space shuttle Atlantis and its six-member crew began an 11-day delivery flight to the International Space Station on Monday, Nov 16, 2009, with a 2:28 p.m. EST launch from NASA's Kennedy S...

  18. Space-Shuttle Emulator Software

    NASA Technical Reports Server (NTRS)

    Arnold, Scott; Askew, Bill; Barry, Matthew R.; Leigh, Agnes; Mermelstein, Scott; Owens, James; Payne, Dan; Pemble, Jim; Sollinger, John; Thompson, Hiram; Thompson, James C.; Walter, Patrick; Brummel, David; Weismuller, Steven P.; Aadsen, Ron; Hurley, Keith; Ruhle, Chris

    2007-01-01

    A package of software has been developed to execute a raw binary image of the space shuttle flight software for simulation of the computational effects of operation of space shuttle avionics. This software can be run on inexpensive computer workstations. Heretofore, it was necessary to use real flight computers to perform such tests and simulations. The package includes a program that emulates the space shuttle orbiter general- purpose computer [consisting of a central processing unit (CPU), input/output processor (IOP), master sequence controller, and buscontrol elements]; an emulator of the orbiter display electronics unit and models of the associated cathode-ray tubes, keyboards, and switch controls; computational models of the data-bus network; computational models of the multiplexer-demultiplexer components; an emulation of the pulse-code modulation master unit; an emulation of the payload data interleaver; a model of the master timing unit; a model of the mass memory unit; and a software component that ensures compatibility of telemetry and command services between the simulated space shuttle avionics and a mission control center. The software package is portable to several host platforms.

  19. Shuttle reentry aerodynamic heating test

    NASA Technical Reports Server (NTRS)

    Pond, J. E.; Mccormick, P. O.; Smith, S. D.

    1971-01-01

    The research for determining the space shuttle aerothermal environment is reported. Brief summaries of the low Reynolds number windward side heating test, and the base and leeward heating and high Reynolds number heating test are included. Also discussed are streamline divergence and the resulting effect on aerodynamic heating, and a thermal analyzer program that is used in the Thermal Environment Optimization Program.

  20. Salt Stress and Ethylene Antagonistically Regulate Nucleocytoplasmic Partitioning of COP1 to Control Seed Germination1[OPEN

    PubMed Central

    Shi, Hui; Gu, Juntao; Dong, Jingao; Deng, Xing Wang

    2016-01-01

    Seed germination, a critical stage initiating the life cycle of a plant, is severely affected by salt stress. However, the underlying mechanism of salt inhibition of seed germination (SSG) is unclear. Here, we report that the Arabidopsis (Arabidopsis thaliana) CONSTITUTIVE PHOTOMORPHOGENESIS1 (COP1) counteracts SSG. Genetic assays provide evidence that SSG in loss of function of the COP1 mutant was stronger than this in the wild type. A GUS-COP1 fusion was constitutively localized to the nucleus in radicle cells. Salt treatment caused COP1 to be retained in the cytosol, but the addition of ethylene precursor 1-aminocyclopropane-1-carboxylate had the reverse effect on the translocation of COP1 to the nucleus, revealing that ethylene and salt exert opposite regulatory effects on the localization of COP1 in germinating seeds. However, loss of function of the ETHYLENE INSENSITIVE3 (EIN3) mutant impaired the ethylene-mediated rescue of the salt restriction of COP1 to the nucleus. Further research showed that the interaction between COP1 and LONG HYPOCOTYL5 (HY5) had a role in SSG. Correspondingly, SSG in loss of function of HY5 was suppressed. Biochemical detection showed that salt promoted the stabilization of HY5, whereas ethylene restricted its accumulation. Furthermore, salt treatment stimulated and ethylene suppressed transcription of ABA INSENSITIVE5 (ABI5), which was directly transcriptionally regulated by HY5. Together, our results reveal that salt stress and ethylene antagonistically regulate nucleocytoplasmic partitioning of COP1, thereby controlling Arabidopsis seed germination via the COP1-mediated down-regulation of HY5 and ABI5. These findings enhance our understanding of the stress response and have great potential for application in agricultural production. PMID:26850275

  1. Unique nucleocytoplasmic dsDNA and +ssRNA viruses are associated with the dinoflagellate endosymbionts of corals

    PubMed Central

    Correa, Adrienne M S; Welsh, Rory M; Vega Thurber, Rebecca L

    2013-01-01

    The residence of dinoflagellate algae (genus: Symbiodinium) within scleractinian corals is critical to the construction and persistence of tropical reefs. In recent decades, however, acute and chronic environmental stressors have frequently destabilized this symbiosis, ultimately leading to coral mortality and reef decline. Viral infection has been suggested as a trigger of coral–Symbiodinium dissociation; knowledge of the diversity and hosts of coral-associated viruses is critical to evaluating this hypothesis. Here, we present the first genomic evidence of viruses associated with Symbiodinium, based on the presence of transcribed +ss (single-stranded) RNA and ds (double-stranded) DNA virus-like genes in complementary DNA viromes of the coral Montastraea cavernosa and expressed sequence tag (EST) libraries generated from Symbiodinium cultures. The M. cavernosa viromes contained divergent viral sequences similar to the major capsid protein of the dinoflagellate-infecting +ssRNA Heterocapsa circularisquama virus, suggesting a highly novel dinornavirus could infect Symbiodinium. Further, similarities to dsDNA viruses dominated (∼69%) eukaryotic viral similarities in the M. cavernosa viromes. Transcripts highly similar to eukaryotic algae-infecting phycodnaviruses were identified in the viromes, and homologs to these sequences were found in two independently generated Symbiodinium EST libraries. Phylogenetic reconstructions substantiate that these transcripts are undescribed and distinct members of the nucleocytoplasmic large DNA virus (NCLDVs) group. Based on a preponderance of evidence, we infer that the novel NCLDVs and RNA virus described here are associated with the algal endosymbionts of corals. If such viruses disrupt Symbiodinium, they are likely to impact the flexibility and/or stability of coral–algal symbioses, and thus long-term reef health and resilience. PMID:22791238

  2. Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport.

    PubMed Central

    Fortes, P; Beloso, A; Ortín, J

    1994-01-01

    The influenza virus RNA segment 8 encodes two proteins, NS1 and NS2, by differential splicing. The collinear transcript acts as mRNA for NS1 protein, while the spliced mRNA encodes NS2 protein. The splicing of NS1 mRNA was studied in cells transfected with a recombinant plasmid that has the cDNA of RNA segment 8 cloned under the SV40 late promoter and polyadenylation signals. As described for influenza virus-infected cells, NS1 mRNA was poorly spliced to yield NS2 mRNA. However, inactivation of the NS1 gene, but not the NS2 gene, led to a substantial increase in the splicing efficiency, as shown by the relative accumulations of NS1 and NS2 mRNAs. This effect was not specific for NS1 mRNA, since the splicing of the endogenous SV40 early transcript was altered in such a way that t-Ag mRNA was almost eliminated. These changes in the splicing pattern coincided with a strong inhibition of the mRNA nucleocytoplasmic transport. Both NS1 and NS2 mRNAs were retained in the nucleus of cells expressing NS1 protein, but no effect was observed when only NS2 protein was expressed. Furthermore, other mRNAs tested, such as T-Ag mRNA and the non-spliceable nucleoprotein transcript, were also retained in the nucleus upon expression of NS1 protein, suggesting that it induced a generalized block of mRNA export from the nucleus. Images PMID:8313914

  3. Space Shuttle Main Engine (SSME) Options for the Future Shuttle

    NASA Technical Reports Server (NTRS)

    Jue, Fred; Kuck, Fritz; McCool, Alex (Technical Monitor)

    2002-01-01

    The main engines for the Future Shuttle will focus on improved safety and operability. Performance enhancements may also be required for vehicle safety purposes to achieve more desirable abort scenarios. This paper discusses the potential improvements that will be considered for implementation into the Future Shuttle. Integrated engine and vehicle health management systems will achieve additional system-level reliability improvements over those currently in development. Advanced instrumentation for detecting leaks, analyzing component wear and degradation, and providing sophisticated operational data will be used for reliable engine control and scheduling maintenance operations. A new nozzle and main combustion chamber (MCC) will reduce failure probability by 50% and allow for higher thrust capability without requiring the entire engine to be redesigned. Turbopump improvements may range from minor component improvements to using 3rd-generation pumps built on the advanced concepts demonstrated by the Integrated Powerhead Development (IPD) program and the Space Launch Initiative (SLI) prototype engines.The main engines for the Future Shuttle will focus on improved safety and operability. Performance enhancements may also be required for vehicle safety purposes to achieve more desirable abort scenarios. This paper discusses the potential improvements that will be considered for implementation into the Future Shuttle. Integrated engine and vehicle health management systems will achieve additional system-level reliability improvements over those currently in development. Advanced instrumentation for detecting leaks, analyzing component wear and degradation, and providing sophisticated operational data will be used for reliable engine control and scheduling maintenance operations. A new nozzle and main combustion chamber (MCC) will reduce failure probability by 50% and allow for higher thrust capability without requiring the entire engine to be redesigned. Turbopump

  4. SESAC statement on shuttle accident

    NASA Astrophysics Data System (ADS)

    The Space and Earth Science Advisory Committee (SESAC) of the NASA Advisory Council (NAC) shares NASA's and the nation's grief in the loss of the Challenger crew—seven exceptional individuals whose lives were dedicated to some of our country's loftiest goals. Over the years, these dedicated individuals and their fellow astronauts have worked closely with the scientific community to ensure that the scientific aspects of the United States space program would be productive in the era of the space shuttle. Through their efforts, the value of manned space flight for accomplishing important research in several areas of space science has been unambiguously demonstrated. Further, as space science has become increasingly an international enterprise, the capabilities of the space shuttle have become central to much scientific planning worldwide.

  5. Digital coding of Shuttle TV

    NASA Technical Reports Server (NTRS)

    Habibi, A.; Batson, B.

    1976-01-01

    Space Shuttle will be using a field-sequential color television system for the first few missions, but the present plans are to switch to a NTSC color TV system for future missions. The field-sequential color TV system uses a modified black and white camera, producing a TV signal with a digital bandwidth of about 60 Mbps. This article discusses the characteristics of the Shuttle TV systems and proposes a bandwidth-compression technique for the field-sequential color TV system that could operate at 13 Mbps to produce a high-fidelity signal. The proposed bandwidth-compression technique is based on a two-dimensional DPCM system that utilizes temporal, spectral, and spatial correlation inherent in the field-sequential color TV imagery. The proposed system requires about 60 watts and less than 200 integrated circuits.

  6. Biowaste monitoring system for shuttle

    NASA Technical Reports Server (NTRS)

    Fogal, G. L.; Sauer, R. L.

    1975-01-01

    The acquisition of crew biomedical data has been an important task on all manned space missions from Project Mercury through the recently completed Skylab Missions. The monitoring of metabolic wastes from the crew is an important aspect of this activity. On early missions emphasis was placed on the collection and return of biowaste samples for post-mission analysis. On later missions such as Skylab, equipment for inflight measurement was also added. Life Science experiments are being proposed for Shuttle missions which will require the inflight measurement and sampling of metabolic wastes. In order to minimize the crew impact associated with these requirements, a high degree of automation of these processes will be required. This paper reviews the design and capabilities of urine biowaste monitoring equipment provided on past-manned space programs and defines and describes the urine volume measurement and sampling equipment planned for the Shuttle Orbiter program.

  7. Space Shuttle Corrosion Protection Performance

    NASA Technical Reports Server (NTRS)

    Curtis, Cris E.

    2007-01-01

    The reusable Manned Space Shuttle has been flying into Space and returning to earth for more than 25 years. The launch pad environment can be corrosive to metallic substrates and the Space Shuttles are exposed to this environment when preparing for launch. The Orbiter has been in service well past its design life of 10 years or 100 missions. As part of the aging vehicle assessment one question under evaluation is how the thermal protection system and aging protective coatings are performing to insure structural integrity. The assessment of this cost resources and time. The information is invaluable when minimizing risk to the safety of Astronauts and Vehicle. This paper will outline a strategic sampling plan and some operational improvements made by the Orbiter Structures team and Corrosion Control Review Board.

  8. Shuttle seated extraction feasibility study

    NASA Technical Reports Server (NTRS)

    Onagel, Steven R.; Bement, Laurence J.

    1989-01-01

    Following the Space Shuttle Challenger accident, serious attention has turned to in-flight escape. Prior to the resumption of flight, a manual bailout system was qualified and installed. For the long term, a seated extraction system to expand the escape envelope is being investigated. This paper describes a 1987 study, conducted jointly by NASA/Johnson Space Center and Langley Research Center, to determine the feasibility of modifying the Space Shuttle Orbiters to incorporate the seated extraction system. Results of the study are positive, indicating retrofit opportunity and high probability of escape for early ascent, late entry, and even for uncontrolled flight such as the Challenger breakup. The system, as envisioned, can extract seven crewmembers within two seconds.

  9. Shuttle seated extraction feasibility study

    NASA Astrophysics Data System (ADS)

    Onagel, Steven R.; Bement, Laurence J.

    Following the Space Shuttle Challenger accident, serious attention has turned to in-flight escape. Prior to the resumption of flight, a manual bailout system was qualified and installed. For the long term, a seated extraction system to expand the escape envelope is being investigated. This paper describes a 1987 study, conducted jointly by NASA/Johnson Space Center and Langley Research Center, to determine the feasibility of modifying the Space Shuttle Orbiters to incorporate the seated extraction system. Results of the study are positive, indicating retrofit opportunity and high probability of escape for early ascent, late entry, and even for uncontrolled flight such as the Challenger breakup. The system, as envisioned, can extract seven crewmembers within two seconds.

  10. Advanced Space Shuttle simulation model

    NASA Technical Reports Server (NTRS)

    Tatom, F. B.; Smith, S. R.

    1982-01-01

    A non-recursive model (based on von Karman spectra) for atmospheric turbulence along the flight path of the shuttle orbiter was developed. It provides for simulation of instantaneous vertical and horizontal gusts at the vehicle center-of-gravity, and also for simulation of instantaneous gusts gradients. Based on this model the time series for both gusts and gust gradients were generated and stored on a series of magnetic tapes, entitled Shuttle Simulation Turbulence Tapes (SSTT). The time series are designed to represent atmospheric turbulence from ground level to an altitude of 120,000 meters. A description of the turbulence generation procedure is provided. The results of validating the simulated turbulence are described. Conclusions and recommendations are presented. One-dimensional von Karman spectra are tabulated, while a discussion of the minimum frequency simulated is provided. The results of spectral and statistical analyses of the SSTT are presented.

  11. Space Shuttle Propulsion Safety Upgrades

    NASA Technical Reports Server (NTRS)

    Humphries, William Randy, Jr.; McCool, Alex (Technical Monitor)

    2000-01-01

    This document is a viewgraph presentation which reviews the proposed upgrades to the Space Shuttle Propulsion system, to improve safety, and reduce significant hazards. The goals of the program are to reduce the risk of a catastrophe in ascent, to achieve significant reduction in orbital and entry systems, and to improve the crew cockpit situational awareness for managing the critical operational situations. The document reviews the upgrades to the propulsion system which are planned to improve the safety. These include modifications to the Advanced Thrust Vector Control, modifications to the Space Shuttle Main Engine Block III, improvement in the Advanced Health Management System, the use of Friction Stir welding on the external tank, which is expected to improve mechanical properties, and reduce defect rate, and the modification of the propellant grains geometry.

  12. Formalizing Space Shuttle Software Requirements

    NASA Technical Reports Server (NTRS)

    Crow, Judith; DiVito, Ben L.

    1996-01-01

    This paper describes two case studies in which requirements for new flight-software subsystems on NASA's Space Shuttle were analyzed, one using standard formal specification techniques, the other using state exploration. These applications serve to illustrate three main theses: (1) formal methods can complement conventional requirements analysis processes effectively, (2) formal methods confer benefits regardless of how extensively they are adopted and applied, and (3) formal methods are most effective when they are judiciously tailored to the application.

  13. The space shuttle at work

    NASA Technical Reports Server (NTRS)

    Allaway, H.

    1979-01-01

    The concept of the orbital flight of the space shuttle and the development of the space transportation system are addressed. How the system came to be, why it is designed the way it is, what is expected of it, and how it may grow are among the questions considered. Emphasis is placed on the effect of the space transportation system on U.S. space exploration in the next decade, including plans to make space an extension of life on the Earth's surface.

  14. Space Shuttle Propulsion Finishing Strong

    NASA Technical Reports Server (NTRS)

    Owen, James W.; Singer, Jody

    2011-01-01

    Numerous lessons have been documented from the Space Shuttle Propulsion elements. Major events include loss of the SRB's on STS-4 and shutdown of an SSME during ascent on STS- 51F. On STS-112 only half the pyrotechnics fired to release the vehicle from the launch pad, a testament for redundancy. STS-91 exhibited freezing of a main combustion chamber pressure measurement and on STS-93 nozzle tube ruptures necessitated a low liquid level oxygen cut off of the main engines. A number of on pad aborts were experienced during the early program resulting in delays. And the two accidents, STS-51L and STS-107, had unique heritage in history from early Program decisions and vehicle configuration. Following STS-51L significant resources were invested in developing fundamental physical understanding of solid rocket motor environments and material system behavior. Human rating of solid rocket motors was truly achieved. And following STS-107, the risk of ascent debris was better characterized and controlled. Situational awareness during all mission phases improved, and the management team instituted effective risk assessment practices. These major events and lessons for the future are discussed. The last 22 flights of the Space Shuttle, following the Columbia accident, were characterized by remarkable improvement in safety and reliability. Numerous problems were solved in addition to reduction of the ascent debris hazard. The propulsion system elements evolved to high reliability and heavy lift capability. The Shuttle system, though not a operable as envisioned in the 1970's, successfully assembled the International Space Station (ISS) and provided significant logistics and down mass for ISS operations. By the end of the Program, the remarkable Space Shuttle Propulsion system achieved very high performance, was largely reusable, exhibited high reliability, and is a heavy lift earth to orbit propulsion system. The story of this amazing system is discussed in detail in the paper.

  15. Shuttle Radar Topography Mission (SRTM)

    USGS Publications Warehouse

    U.S. Geological Survey

    2009-01-01

    Under an agreement with the National Aeronautics and Space Administration (NASA) and the Department of Defense's National Geospatial-Intelligence Agency (NGA), the U.S. Geological Survey (USGS) is distributing elevation data from the Shuttle Radar Topography Mission (SRTM). The SRTM is a joint project of NASA and NGA to map the Earth's land surface in three dimensions at an unprecedented level of detail. As part of space shuttle Endeavour's flight during February 11-22, 2000, the SRTM successfully collected data over 80 percent of the Earth's land surface for most of the area between latitudes 60 degrees north and 56 degrees south. The SRTM hardware included the Spaceborne Imaging Radar-C (SIR-C) and X-band Synthetic Aperture Radar (X-SAR) systems that had flown twice previously on other space shuttle missions. The SRTM data were collected with a technique known as interferometry that allows image data from dual radar antennas to be processed for the extraction of ground heights.

  16. Shuttle Radar Topography Mission (SRTM)

    USGS Publications Warehouse

    U.S. Geological Survey

    2003-01-01

    Under an agreement with the National Aeronautics and Space Administration (NASA) and the Department of Defense's National Imagery and Mapping Agency (NIMA), the U.S. Geological Survey (USGS) is now distributing elevation data from the Shuttle Radar Topography Mission (SRTM). The SRTM is a joint project between NASA and NIMA to map the Earth's land surface in three dimensions at a level of detail unprecedented for such a large area. Flown aboard the NASA Space Shuttle Endeavour February 11-22, 2000, the SRTM successfully collected data over 80 percent of the Earth's land surface, for most of the area between 60? N. and 56? S. latitude. The SRTM hardware included the Spaceborne Imaging Radar-C (SIR-C) and X-band Synthetic Aperture Radar (X-SAR) systems that had flown twice previously on other space shuttle missions. The SRTM data were collected specifically with a technique known as interferometry that allows image data from dual radar antennas to be processed for the extraction of ground heights.

  17. Shuttle accident stalls science plans

    NASA Astrophysics Data System (ADS)

    Katzoff, Judith A.

    Plans to make 1986 a uniquely productive year for U.S. space science activities ended in one horrible moment with the January 28, 1986, explosion of the space shuttle Challenger. The joyless scene at Cape Canaveral, Fla., stood in sharp contrast to the overwhelming success of Voyager 2 in its encounter with Uranus 4 days earlier. (Scientific details of that encounter will follow in upcoming issues of Eos.)Of the 15 space shuttle flights planned for fiscal year 1986, beginning October 1, 1985, a total of seven were to have carried scientific payloads for the National Aeronautics and Space Administration (NASA). The remaining eight flights were evenly divided between missions for the U.S. Department of Defense and commercial missions for NASA's paying customers. The explosion caused NASA to put its entire space shuttle program on hold to allow time for engineers to find the cause of the accident and for NASA to implement corrective measures. As Eos went to press, NASA acting administrator William R. Graham had not yet released the names of those who would serve on the formal investigative panel. “I think everybody's agreed that it will take weeks to months to unravel,” said Alexander Dessler, director of the space science laboratory at NASA's Marshall Space Flight Center near Huntsville, Ala. Dessler speculated that investigators would begin with a list of hundreds of possible causes for the explosion.

  18. Survey of ultraviolet shuttle glow

    NASA Technical Reports Server (NTRS)

    Spear, K. A.; Uckler, G. J.; Tobiska, K.

    1985-01-01

    The University of Colorado Get Away Special (GAS) project utilizes the efforts of its students to place experiments on the shuttle. The objective of one experiment, the shuttle glow study, is to conduct a general survey of emissions in the ultraviolet near vehicle surfaces. An approximate wavelength range of 1900 to 3000 A will be scanned to observe predominant features. Special emphasis will be placed on studying the band structure of NO near 2000 A and the Mg+ line at 2800 A. The spectrometer, of Ebert-Faste 1/8-meter design, will perform the experiment during spacecraft night. It will be oriented such that the optical axis points to the cargo bay zenith. In order to direct the field-of-view of the instrument onto the shuttle vertical stabilizer (tail), a mirror assembly is employed. The mirror system has been designed to rotate through 7.5 degrees of arc using 10 positions resulting in a spatial resolution of 30 x 3 cm, with the larger dimension corresponding to the horizontal direction. Such a configuration can be attained from the forwardmost position in the cargo bay. Each spatial position will be subjected to a full spectral scan with a resolution on the order of 10 A.

  19. Space Shuttle Star Tracker Challenges

    NASA Technical Reports Server (NTRS)

    Herrera, Linda M.

    2010-01-01

    The space shuttle fleet of avionics was originally designed in the 1970's. Many of the subsystems have been upgraded and replaced, however some original hardware continues to fly. Not only fly, but has proven to be the best design available to perform its designated task. The shuttle star tracker system is currently flying as a mixture of old and new designs, each with a unique purpose to fill for the mission. Orbiter missions have tackled many varied missions in space over the years. As the orbiters began flying to the International Space Station (ISS), new challenges were discovered and overcome as new trusses and modules were added. For the star tracker subsystem, the growing ISS posed an unusual problem, bright light. With two star trackers on board, the 1970's vintage image dissector tube (IDT) star trackers track the ISS, while the new solid state design is used for dim star tracking. This presentation focuses on the challenges and solutions used to ensure star trackers can complete the shuttle missions successfully. Topics include KSC team and industry partner methods used to correct pressurized case failures and track system performance.

  20. Shuttle/Centaur project perspective

    NASA Technical Reports Server (NTRS)

    Muckley, E. T.

    1984-01-01

    The shuttle/Centaur vehicle is being developed as an expendable, cryogenic high energy upper stage for use with the National Space Transportation System (NSTS). The stage is expected to meet the demands of a wide range of users. The shuttle/Centaur will be a modification of the highly successful Centaur stage, used extensively with the Atlas and Titan boosters since 1966 to launch planetary, geosynchronous and Earth orbital missions. The design changes required for use with the NSTS are described. These are primarily related to: (1) tank resizing to take advantage of the orbiter payload bay dimensions; (2) provisions for physically adopting Centaur to the orbiter; and, (3) accommodating safety requirements of the manned NSTS. The expected performance capabilities of two versions of the shuttle/Centaur are also described. The initial version, designated G-prime, is the larger of the two, with a length of about 9.1m (30 ft.). This vehicle will be used to launch the Galileo and International Solar Polar Mission to Jupiter in May 1986.

  1. Simulated Shuttle Egress: Comparison of Two Space Shuttle Protective Garments

    NASA Technical Reports Server (NTRS)

    Lee, Stuart M.C.; Bishop, Phillip A.; Schneider, Suzanne M.; Greenisen, Michael C.; Paloski, William H. (Technical Monitor)

    2000-01-01

    In a previous study from our laboratory, we observed carbon dioxide (CO2) accumulation in the helmet of the NASA Launch and Entry Suit (LES) during a simulated emergency egress from the Space Shuttle. Eight of 12 subjects were unable to complete the egress simulation with a G-suit inflation pressure of 1.5 psi. The purpose of this report was to compare CO2 accumulation and egress walking time in the new Advanced Crew Escape Suit (ACES) with that in the LES.

  2. Nucleocytoplasmic Translocation of UBXN2A Is Required for Apoptosis during DNA Damage Stresses in Colon Cancer Cells

    PubMed Central

    Abdullah, Ammara; Sane, Sanam; Freeling, Jessica L; Wang, Hongmin; Zhang, Dong; Rezvani, Khosrow

    2015-01-01

    The subcellular localization, expression level, and activity of anti-cancer proteins alter in response to intrinsic and extrinsic cellular stresses to reverse tumor progression. The purpose of this study is to determine whether UBXN2A, an activator of the p53 tumor suppressor protein, has different subcellular compartmentalization in response to the stress of DNA damage. We measured trafficking of the UBXN2A protein in response to two different DNA damage stresses, UVB irradiation and the genotoxic agent Etoposide, in colon cancer cell lines. Using a cytosol-nuclear fractionation technique followed by western blot and immunofluorescence staining, we monitored and quantitated UBXN2A and p53 proteins as well as p53's downstream apoptotic pathway. We showed that the anti-cancer protein UBXN2A acts in the early phase of cell response to two different DNA damage stresses, being induced to translocate into the cytoplasm in a dose- and time-dependent manner. UVB-induced cytoplasmic UBXN2A binds to mortalin-2 (mot-2), a known oncoprotein in colon tumors. UVB-dependent upregulation of UBXN2A in the cytoplasm decreases p53 binding to mot-2 and activates apoptotic events in colon cancer cells. In contrast, the shRNA-mediated depletion of UBXN2A leads to significant reduction in apoptosis in colon cancer cells exposed to UVB and Etoposide. Leptomycin B (LMB), which was able to block UBXN2A nuclear export following Etoposide treatment, sustained p53-mot-2 interaction and had partially antagonistic effects with Etoposide on cell apoptosis. The present study shows that nucleocytoplasmic translocation of UBXN2A in response to stresses is necessary for its anti-cancer function in the cytoplasm. In addition, LMB-dependent suppression of UBXN2A's translocation to the cytoplasm upon stress allows the presence of an active mot-2 oncoprotein in the cytoplasm, resulting in p53 sequestration as well as activation of other mot-2-dependent growth promoting pathways. PMID:26516353

  3. Experiment definition phase shuttle laboratory (LDRL-10.6 experiment): Shuttle sortie to elliptical orbit satellite

    NASA Technical Reports Server (NTRS)

    Goodwin, F. E.; Nussmeier, T. A.; Stokes, L. S.; Vourgourakis, E. J.

    1976-01-01

    The following topics were reviewed: (1) design options for shuttle terminal, (2) elliptical orbit satellite design options, (3) shuttle terminal details, (4) technology status and development requirements, (5) transmitter technology, and (6) carbon dioxide laser life studies.

  4. Evaluation philosophy for shuttle launched payloads

    NASA Technical Reports Server (NTRS)

    Heuser, R. E.

    1975-01-01

    Some approaches to space-shuttle payload evaluation are examined. Issues considered include subsystem replacement in low-cost modular spacecraft (LCMS), validation of spacelab payloads, the use of standard components in shuttle-era spacecraft, effects of shuttle-induced environments on payloads, and crew safety. The LCMS is described, and goals are discussed for its evaluation program. Concepts regarding how the evaluation should proceed are considered.

  5. Laboratory investigation of shuttle glow mechanisms

    NASA Astrophysics Data System (ADS)

    Caledonia, G. E.; Holtzclaw, K. W.; Green, B. D.; Krech, R. H.; Leone, A.; Swenson, G.

    1990-10-01

    A fast oxygen atom source was used to investigate the Shuttle glow phenomena in the laboratory. Both room temperature and cooled targets were dosed with NO and then irradiated by 8 km/s oxygen atoms. The observed fluorescence is spectrally similar to that seen on the Shuttle supporting previous suggestions that recombination of O with surface bound NO is the responsible mechanism for the visible Shuttle glow.

  6. Shuttle being tested at Marshall Center

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Ground vibration tests of the space shuttle vehicle performed to evaluate the structural dynamics and their effect on the control system of the shuttle are described. Test results are used to verify the system design and mathematical models that predict how the shuttle's control system will react to the much more severe vibrations expected during launch and flight into orbit. The test configurations, the test facility, and the dynamic test suspension system are among the topics discussed.

  7. Space Shuttle Usage of z/OS

    NASA Technical Reports Server (NTRS)

    Green, Jan

    2009-01-01

    This viewgraph presentation gives a detailed description of the avionics associated with the Space Shuttle's data processing system and its usage of z/OS. The contents include: 1) Mission, Products, and Customers; 2) Facility Overview; 3) Shuttle Data Processing System; 4) Languages and Compilers; 5) Application Tools; 6) Shuttle Flight Software Simulator; 7) Software Development and Build Tools; and 8) Fun Facts and Acronyms.

  8. STS-80 Space Shuttle Mission Report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1997-01-01

    The STS-80 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the eightieth flight of the Space Shuttle Program, the fifty-fifth flight since the return-to-flight, and the twenty-first flight of the Orbiter Columbia (OV-102).

  9. Space Shuttle Main Engine (SSME) Test Firing

    NASA Technical Reports Server (NTRS)

    1981-01-01

    A Space Shuttle Main Engine (SSME) undergoing a full power level 290.04 second test firing at the National Space Technology Laboratories (currently called the Stennis Space Center) in Mississippi. The firings were part of a series of developmental testing designed to increase the amount of thrust available to the Shuttle from its three main engines. The additional thrust allowed the Shuttle to launch heavier payloads into orbit. The Marshall Space Flight Center (MSFC) had management responsibility of Space Shuttle propulsion elements, including the Main Engines.

  10. Space Shuttle Main Engine Public Test Firing

    NASA Technical Reports Server (NTRS)

    2000-01-01

    A new NASA Space Shuttle Main Engine (SSME) roars to the approval of more than 2,000 people who came to John C. Stennis Space Center in Hancock County, Miss., on July 25 for a flight-certification test of the SSME Block II configuration. The engine, a new and significantly upgraded shuttle engine, was delivered to NASA's Kennedy Space Center in Florida for use on future shuttle missions. Spectators were able to experience the 'shake, rattle and roar' of the engine, which ran for 520 seconds - the length of time it takes a shuttle to reach orbit.

  11. Electromagnetic Compatibility for the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Scully, Robert C.

    2004-01-01

    This slide presentation reviews the Space Shuttle electromagnetic compatibility (EMC). It includes an overview of the design of the shuttle with the areas that are of concern for the electromagnetic compatibility. It includes discussion of classical electromagnetic interference (EMI) and the work performed to control the electromagnetic interference. Another area of interest is electrostatic charging and the threat of electrostatic discharge and the attempts to reduce damage to the Shuttle from these possible hazards. The issue of electrical bonding is als reviewed. Lastly the presentation reviews the work performed to protect the shuttle from lightning, both in flight and on the ground.

  12. Space Shuttle aerothermodynamic data report, phase C

    NASA Technical Reports Server (NTRS)

    1985-01-01

    Space shuttle aerothermodynamic data, collected from a continuing series of wind tunnel tests, are permanently stored with the Data Management Services (DMS) system. Information pertaining to current baseline configuration definition is also stored. Documentation of DMS processed data arranged sequentially and by space shuttle configuration are included. An up-to-date record of all applicable aerothermodynamic data collected, processed, or summarized during the space shuttle program is provided. Tables are designed to provide suvery information to the various space shuttle managerial and technical levels.

  13. Shuttle Enterprise Being Worked on in Hangar

    NASA Technical Reports Server (NTRS)

    1983-01-01

    The Space Shuttle Enterprise being worked on in the weight & balance hangar. The Enterprise, the first orbiter built, was not spaceflight rated and was used in 1977 to verify the landing, approach, and glide characteristics of the orbiters in the Approach and Landing Tests (ALT) at Edwards Air Force Base, California. It was also used for engineering fit-checks at the shuttle launch facilities. Following approach and landing tests in 1977 and its use as an engineering vehicle, Enterprise was donated to the National Air and Space Museum in Washington, D.C. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the laboratories remain inside the payload bay throughout the mission. They are then removed after the Space Shuttle returns to Earth and can be reused on future flights. Some of these orbital

  14. Space Shuttle and Hypersonic Entry

    NASA Technical Reports Server (NTRS)

    Campbell, Charles H.; Gerstenmaier, William H.

    2014-01-01

    Fifty years of human spaceflight have been characterized by the aerospace operations of the Soyuz, of the Space Shuttle and, more recently, of the Shenzhou. The lessons learned of this past half decade are important and very significant. Particularly interesting is the scenario that is downstream from the retiring of the Space Shuttle. A number of initiatives are, in fact, emerging from in the aftermath of the decision to terminate the Shuttle program. What is more and more evident is that a new era is approaching: the era of the commercial usage and of the commercial exploitation of space. It is probably fair to say, that this is the likely one of the new frontiers of expansion of the world economy. To make a comparison, in the last 30 years our economies have been characterized by the digital technologies, with examples ranging from computers, to cellular phones, to the satellites themselves. Similarly, the next 30 years are likely to be characterized by an exponential increase of usage of extra atmospheric resources, as a result of more economic and efficient way to access space, with aerospace transportation becoming accessible to commercial investments. We are witnessing the first steps of the transportation of future generation that will drastically decrease travel time on our Planet, and significantly enlarge travel envelope including at least the low Earth orbits. The Steve Jobs or the Bill Gates of the past few decades are being replaced by the aggressive and enthusiastic energy of new entrepreneurs. It is also interesting to note that we are now focusing on the aerospace band, that lies on top of the aeronautical shell, and below the low Earth orbits. It would be a mistake to consider this as a known envelope based on the evidences of the flights of Soyuz, Shuttle and Shenzhou. Actually, our comprehension of the possible hypersonic flight regimes is bounded within really limited envelopes. The achievement of a full understanding of the hypersonic flight

  15. STS-105 Shuttle Orbiter Discovery

    NASA Technical Reports Server (NTRS)

    2001-01-01

    This is a view of the Space Shuttle Discovery as it approaches the International Space Station (ISS) during the STS-105 mission. Visible in the payload bay of Discovery are the Multipurpose Logistics Module (MPLM) Leonardo at right, which stores various supplies and experiments to be transferred into the ISS; at center, the Integrated Cargo Carrier (ICC) which carries the Early Ammonia Servicer (EAS); and two Materials International Space Station Experiment (MISSE) containers at left. Aboard Discovery were the ISS Expedition Three crew, who were to replace the Expedition Two crew that had been living on the ISS for the past five months.

  16. Shuttle bioresearch laboratory breadboard simulations

    NASA Technical Reports Server (NTRS)

    Taketa, S. T.

    1975-01-01

    Laboratory breadboard simulations (Tests I and II) were conducted to test concepts and assess problems associated with bioresearch support equipment, facilities, and operational integration for conducting manned earth orbital Shuttle missions. This paper describes Test I and discusses the major observations made in Test II. The tests emphasized candidate experiment protocols and requirements: Test I for biological research and Test II for crew members (simulated), subhuman primates, and radioisotope tracer studies on lower organisms. The procedures and approaches developed for these simulation activities could form the basis for Spacelab simulations and developing preflight integration, testing, and logistics of flight payloads.

  17. Seismic excitation by space shuttles

    USGS Publications Warehouse

    Kanamori, H.; Mori, J.; Sturtevant, B.; Anderson, D.L.; Heaton, T.

    1992-01-01

    Shock waves generated by the space shuttles Columbia (August 13, 1989), Atlantis (April 11, 1991) and Discovery (September 18, 1991) on their return to Edwards Air Force Base, California, were recorded by TERRAscope (Caltech's broadband seismic network), the Caltech-U.S.G.S Southern California Seismic Network (SCSN), and the University of Southern California (USC) Los Angeles Basin Seismic Network. The spatial pattern of the arrival times exhibits hyperbolic shock fronts from which the path, velocity and altitude of the space shuttle could be determined. The shock wave was acoustically coupled to the ground, converted to a seismic wave, and recorded clearly at the broadband TERRAscope stations. The acoustic coupling occurred very differently depending on the conditions of the Earth's surface surrounding the station. For a seismic station located on hard bedrock, the shock wave (N wave) was clearly recorded with little distortion. Aside from the N wave, very little acoustic coupling of the shock wave energy to the ground occurred at these sites. The observed N wave record was used to estimate the overpressure of the shock wave accurately; a pressure change of 0.5 to 2.2 mbars was obtained. For a seismic station located close to the ocean or soft sedimentary basins, a significant amount of shock wave energy was transferred to the ground through acoustic coupling of the shock wave and the oceanic Rayleigh wave. A distinct topography such as a mountain range was found effective to couple the shock wave energy to the ground. Shock wave energy was also coupled to the ground very effectively through large man made structures such as high rise buildings and offshore oil drilling platforms. For the space shuttle Columbia, in particular, a distinct pulse having a period of about 2 to 3 seconds was observed, 12.5 s before the shock wave, with a broadband seismograph in Pasadena. This pulse was probably excited by the high rise buildings in downtown Los Angeles which were

  18. Shuttle Discovery Mated to 747 SCA

    NASA Technical Reports Server (NTRS)

    1983-01-01

    The Space Shuttle Discovery rides atop '905,' NASA's 747 Shuttle Carrier Aircraft, on its delivery flight from California to the Kennedy Space Center, Florida, where it was prepared for its first orbital mission for 30 August to 5 September 1984. The NASA 747, obtained in 1974, has special support struts atop the fuselage and internal strengthening to accommodate the additional weight of the orbiters. Small vertical fins have also been added to the tips of the horizontal stabilizers for additional stability due to air turbulence on the control surfaces caused by the orbiters. A second modified 747, no. 911, went in to service in November 1990 and is also used to ferry orbiters to destinations where ground transportation is not practical. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space Shuttles are designed to be continually reused. When Space Shuttles are used to transport complete scientific laboratories into space, the

  19. Shuttle Orbital Applications and Requirements, supplementary tasks (SOAR-IIS)

    NASA Technical Reports Server (NTRS)

    1973-01-01

    Representative shuttle mission applications were studied. The interfaces analyses, and specific payloads are reported for the following types of missions: shuttle delivered automated spacecraft, shuttle/tug delivered spacecraft, man-tended automated spacecraft, and sortie missions.

  20. Space Shuttle Status News Conference

    NASA Technical Reports Server (NTRS)

    2005-01-01

    Richard Gilbech, External Tank "Tiger Team" Lead, begins this space shuttle news conference with detailing the two major objectives of the team. The objectives include: 1) Finding the root cause of the foam loss on STS-114; and 2) Near and long term improvements for the external tank. Wayne Hale, Space Shuttle Program Manager, presents a chart to explain the external tank foam loss during STS-114. He gives a possible launch date for STS-121 after there has been a repair to the foam on the External Tank. He further discusses the changes that need to be made to the surrounding areas of the plant in New Orleans, due to Hurricane Katrina. Bill Gerstemaier, NASA Associate Administrator for Space Operations, elaborates on the testing of the external tank foam loss. The discussion ends with questions from the news media about a fix for the foam, replacement of the tiles, foam loss avoidance, the root cause of foam loss and a possible date for a new external tank to be shipped to NASA Kennedy Space Center.

  1. STS-109 Shuttle Mission Launch

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Carrying the STS-109 crew of seven, the Space Shuttle Orbiter Columbia blasted from its launch pad as it began its 27th flight and 108th flight overall in NASA's Space Shuttle Program. Launched March 1, 2002, the goal of the mission was the maintenance and upgrade of the Hubble Space Telescope (HST) which was developed, designed, and constructed by the Marshall Space Flight Center. Captured and secured on a work stand in Columbia's payload bay using Columbia's robotic arm, the HST received the following upgrades: replacement of the solar array panels; replacement of the power control unit (PCU); replacement of the Faint Object Camera (FOC) with a new advanced camera for Surveys (ACS); and installation of the experimental cooling system for the Hubble's Near-Infrared Camera and Multi-object Spectrometer (NICMOS), which had been dormant since January 1999 when it original coolant ran out. Four of the crewmembers performed 5 space walks in the 10 days, 22 hours, and 11 minutes of the the STS-109 mission.

  2. The 1995 Shuttle Small Payloads Symposium

    NASA Technical Reports Server (NTRS)

    Goldsmith, Frann (Editor); Mosier, Frances L. (Editor)

    1995-01-01

    The 1995 Shuttle Small Payloads Symposium is a combined symposia of the Get Away Special (GAS) and Hitchhiker programs, and is proposed to continue as an annual conference. The focus of this conference is to educate potential Space Shuttle Payload Bay users as to the types of carrier systems provided and for current users to share experiment concepts.

  3. Space science plans for the shuttle era.

    NASA Technical Reports Server (NTRS)

    Naugle, J. E.; Johnson, R. W.

    1972-01-01

    Review of the current thinking on the space science, exploration, and application plans and policies that are to take advantage of the widened capabilities to be provided by the shuttle and the sortie mode of the shuttle. Present planning activities and plans for the next year are discussed.

  4. The 1992 Shuttle Small Payloads Symposium

    NASA Technical Reports Server (NTRS)

    Thomas, Lawrence R. (Editor); Mosier, Frances L. (Editor)

    1992-01-01

    The 1992 Shuttle Small Payloads Symposium is a continuation of the Get Away Special Symposium convened from 1984 through 1988, and is proposed to continue as an annual conference. The focus of this conference is to educate potential Space Shuttle Payload Bay users as to the types of carrier systems provided and for current users to share experiment concepts.

  5. Liftoff of STS-59 Shuttle Endeavour

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The liftoff of the Space Shuttle Endeavour is backdropped against clouds at the Kennedy Space Center (KSC). Liftoff occurred at 7:05 a.m., April 9, 1994. The air-to-air view was photographed from the Shuttle Training Aircraft (STA) piloted by astronaut Robert L. Gibson.

  6. Launch of STS-67 Space Shuttle Endeavour

    NASA Technical Reports Server (NTRS)

    1995-01-01

    Carrying a crew of seven and a complement of astronomic experiments, the Space Shuttle Endeavour embarks on NASA's longest shuttle flight to date. Endeavour's liftoff from Launch Pad 39A occurred at 1:38:13 a.m. (EST), March 2, 1995. In this view the fence line near the launch pad is evident in the foreground.

  7. Liftoff of STS-67 Space Shuttle Endeavour

    NASA Technical Reports Server (NTRS)

    1995-01-01

    Carrying a crew of seven and a complement of astronomic experiments, the Space Shuttle Endeavour embarks on NASA's longest Shuttle flight to date. Endeavour's liftoff from Launch Pad 39A occurred at 1:38:13 a.m. (EST), March 2, 1995.

  8. Liftoff of STS-67 Space Shuttle Endeavour

    NASA Technical Reports Server (NTRS)

    1995-01-01

    Carrying a crew of seven and a compliment of astronomic experiments, the Space Shuttle Endeavour embarks on NASA's longest Shuttle flight to date. Endeavour's liftoff from Launch Pad 39A occurred at 1:38:13 a.m. (EST), March 2, 1995.

  9. The 1993 Shuttle Small Payloads Symposium

    NASA Technical Reports Server (NTRS)

    Thomas, Lawrence R. (Editor); Mosier, Frances L. (Editor)

    1993-01-01

    The 1993 Shuttle Small Payloads Symposium is a combined symposia of the Get Away Special (GAS), Hitchhiker, and Complex Autonomous Payloads (CAP) programs, and is proposed to continue as an annual conference. The focus of this conference is to educate potential Space Shuttle Payload Bay users as to the types of carrier systems provided and for current users to share experiment concepts.

  10. Restartable solid motor stage for shuttle applications

    NASA Technical Reports Server (NTRS)

    Rohrbaugh, D. J.

    1973-01-01

    The application of restartable solid motor stages to shuttle missions has been shown to provide a viable supplement to the shuttle program. Restartable solid motors in the 3000 pound class provide a small expendable transfer stage that reduces the demand on the shuttle for the lower energy missions. Shuttle operational requirements and preliminary performance data provided an input for defining design features required for restartable solid motor applications. These data provided a basis for a configuration definition that is compatible with shuttle operations. Mission by mission analysis showed the impact on a NASA supplied mission model. The results showed a 15% reduction in the number of shuttle flights required. In addition the amount of shuttle capability used to complete the mission objectives was significantly reduced. For example, in the 1979 missions there was a 62% reduction in shuttle capability used. The study also showed that the solid motor could provide a supplement to the TUG that would allow TUGS to be used in a recoverable rather than an expendable mode. The study shows a 71% reduction in the number of TUGs that would be expended.

  11. ATLAS Series of Shuttle Missions. Volume 23

    NASA Technical Reports Server (NTRS)

    1996-01-01

    This technical paper contains selected papers from Geophysical Research Letters (Volume 23, Number 17) on ATLAS series of shuttle missions. The ATLAS space shuttle missions were conducted in March 1992, April 1993, and November 1994. This paper discusses solar irradiance, middle atmospheric temperatures, and trace gas concentrations measurements made by the ATLAS payload and companion instruments.

  12. Metallic materials for the space shuttle.

    NASA Technical Reports Server (NTRS)

    Tavassoli, A. A.

    1972-01-01

    The need for further development and reevaluation of current space-vehicle materials (intended for a single mission) to meet the long-time requirements of a space shuttle is demonstrated. Leading metallic material candidates and their properties are tabulated for a delta-wing space shuttle configuration with a metallic thermal protection system.

  13. Launch Vehicle Demonstrator Using Shuttle Assets

    NASA Technical Reports Server (NTRS)

    Creech, Dennis M.; Threet, Grady E., Jr.; Waters, Eric D.

    2011-01-01

    Study Objective is to characterize the performance capabilities of an inline, shuttle-derived launch vehicle using two design strategies: the first as an early program demonstrator utilizing high structural margins, maximum shuttle assets, and minimal pad impact, the later having undergone structural optimization, flying operational mission GR&A and serving as a baseline for evolutionary upgrades.

  14. Liquid Hydrogen Consumption During Space Shuttle Program

    NASA Technical Reports Server (NTRS)

    Partridge, Jonathan K.

    2011-01-01

    This slide presentation reviews the issue of liquid hydrogen consumption and the points of its loss in prior to the shuttle launch. It traces the movement of the fuel from the purchase to the on-board quantity and the loss that results in 54.6 of the purchased quantity being on board the Shuttle.

  15. STS-55 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1993-01-01

    A summary of the Space Shuttle Payloads, Orbiter, External Tank, Solid Rocket Booster, Redesigned Solid Rocket Motor, and the Main Engine subsystems performance during the 55th flight of the Space Shuttle Program and the 14th flight of Columbia is presented.

  16. Space Shuttle communications and tracking system

    NASA Technical Reports Server (NTRS)

    Tu, Kwei; Cravey, Donald N.; Kuo, Yeng S.; Johnson, John H.; Teasdale, William E.

    1987-01-01

    This paper is intended to provide a comprehensive description of the Space Shuttle communications and tracking system design, development, and system operations. Areas of special innovative communications techniques implemented by the Space Shuttle such as source encoding, channel encoding, spread spectrum, and digital modulation are emphasized. In addition, the role of the Electronics Systems Test Laboratory in the development of the system is discussed.

  17. Space shuttle orbiter test flight series

    NASA Technical Reports Server (NTRS)

    Garrett, D.; Gordon, R.; Jackson, R. B.

    1977-01-01

    The proposed studies on the space shuttle orbiter test taxi runs and captive flight tests were set forth. The orbiter test flights, the approach and landing tests (ALT), and the ground vibration tests were cited. Free flight plans, the space shuttle ALT crews, and 747 carrier aircraft crew were considered.

  18. Report of the Shuttle Processing Review Team

    NASA Technical Reports Server (NTRS)

    1993-01-01

    The intent of this report is to summarize the assessment of the shuttle processing operations at the Kennedy Space Center (KSC) as requested by the NASA Administrator. He requested a team reaffirmation that safety is the number one priority and review operations to ensure confidence in the shuttle processing procedures at KSC.

  19. Shuttle Entry Imaging Using Infrared Thermography

    NASA Technical Reports Server (NTRS)

    Horvath, Thomas; Berry, Scott; Alter, Stephen; Blanchard, Robert; Schwartz, Richard; Ross, Martin; Tack, Steve

    2007-01-01

    During the Columbia Accident Investigation, imaging teams supporting debris shedding analysis were hampered by poor entry image quality and the general lack of information on optical signatures associated with a nominal Shuttle entry. After the accident, recommendations were made to NASA management to develop and maintain a state-of-the-art imagery database for Shuttle engineering performance assessments and to improve entry imaging capability to support anomaly and contingency analysis during a mission. As a result, the Space Shuttle Program sponsored an observation campaign to qualitatively characterize a nominal Shuttle entry over the widest possible Mach number range. The initial objectives focused on an assessment of capability to identify/resolve debris liberated from the Shuttle during entry, characterization of potential anomalous events associated with RCS jet firings and unusual phenomenon associated with the plasma trail. The aeroheating technical community viewed the Space Shuttle Program sponsored activity as an opportunity to influence the observation objectives and incrementally demonstrate key elements of a quantitative spatially resolved temperature measurement capability over a series of flights. One long-term desire of the Shuttle engineering community is to calibrate boundary layer transition prediction methodologies that are presently part of the Shuttle damage assessment process using flight data provided by a controlled Shuttle flight experiment. Quantitative global imaging may offer a complementary method of data collection to more traditional methods such as surface thermocouples. This paper reviews the process used by the engineering community to influence data collection methods and analysis of global infrared images of the Shuttle obtained during hypersonic entry. Emphasis is placed upon airborne imaging assets sponsored by the Shuttle program during Return to Flight. Visual and IR entry imagery were obtained with available airborne

  20. Space Shuttle wind tunnel testing program

    NASA Technical Reports Server (NTRS)

    Whitnah, A. M.; Hillje, E. R.

    1984-01-01

    A major phase of the Space Shuttle Vehicle (SSV) Development Program was the acquisition of data through the space shuttle wind tunnel testing program. It became obvious that the large number of configuration/environment combinations would necessitate an extremely large wind tunnel testing program. To make the most efficient use of available test facilities and to assist the prime contractor for orbiter design and space shuttle vehicle integration, a unique management plan was devised for the design and development phase. The space shuttle program is reviewed together with the evolutional development of the shuttle configuration. The wind tunnel testing rationale and the associated test program management plan and its overall results is reviewed. Information is given for the various facilities and models used within this program. A unique posttest documentation procedure and a summary of the types of test per disciplines, per facility, and per model are presented with detailed listing of the posttest documentation.

  1. STS-38 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Camp, David W.; Germany, D. M.; Nicholson, Leonard S.

    1991-01-01

    The STS-38 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities on this thirty-seventh flight of the Space Shuttle and the seventh flight of the Orbiter vehicle Atlantis (OV-104). In addition to the Atlantis vehicle, the flight vehicle consisted of an External Tank (ET) (designated as ET-40/LWT-33), three Space Shuttle main engines (SSME's) (serial numbers 2019, 2022, 2027), and two Solid Rocket Boosters (SRB's), designated as BI-039. The STS-38 mission was a classified Department of Defense mission, and as much, the classified portions of the mission are not presented in this report. The sequence of events for this mission is shown. The significant problems that occurred in the Space Shuttle Orbiter subsystem during the mission are summarized and the official problem tracking list is presented. In addition, each Space Shuttle Orbiter problem is cited in the subsystem discussion.

  2. Shuttle Carrier Aircraft (SCA) Fleet Photo

    NASA Technical Reports Server (NTRS)

    1995-01-01

    NASA's two Boeing 747 Shuttle Carrier Aircraft (SCA) are seen here nose to nose at Dryden Flight Research Center, Edwards, California. The front mounting attachment for the Shuttle can just be seen on top of each. The SCAs are used to ferry Space Shuttle orbiters from landing sites back to the launch complex at the Kennedy Space Center, and also to and from other locations too distant for the orbiters to be delivered by ground transportation. The orbiters are placed atop the SCAs by Mate-Demate Devices, large gantry-like structures which hoist the orbiters off the ground for post-flight servicing, and then mate them with the SCAs for ferry flights. Features which distinguish the two SCAs from standard 747 jetliners are; three struts, with associated interior structural strengthening, protruding from the top of the fuselage (two aft, one forward) on which the orbiter is attached, and two additional vertical stabilizers, one on each end of the standard horizontal stabilizer, to enhance directional stability. The two SCAs are under the operational control of NASA's Johnson Space Center, Houston, Texas. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back

  3. Wavelet Regularization Per Nullspace Shuttle

    NASA Astrophysics Data System (ADS)

    Charléty, J.; Nolet, G.; Sigloch, K.; Voronin, S.; Loris, I.; Simons, F. J.; Daubechies, I.; Judd, S.

    2010-12-01

    Wavelet decomposition of models in an over-parameterized Earth and L1-norm minimization in wavelet space is a promising strategy to deal with the very heterogeneous data coverage in the Earth without sacrificing detail in the solution where this is resolved (see Loris et al., abstract this session). However, L1-norm minimizations are nonlinear, and pose problems of convergence speed when applied to large data sets. In an effort to speed up computations we investigate the application of the nullspace shuttle (Deal and Nolet, GJI 1996). The nullspace shuttle is a filter that adds components from the nullspace to the minimum norm solution so as to have the model satisfy additional conditions not imposed by the data. In our case, the nullspace shuttle projects the model on a truncated basis of wavelets. The convergence of this strategy is unproven, in contrast to algorithms using Landweber iteration or one of its variants, but initial computations using a very large data base give reason for optimism. We invert 430,554 P delay times measured by cross-correlation in different frequency windows. The data are dominated by observations with US Array, leading to a major discrepancy in the resolution beneath North America and the rest of the world. This is a subset of the data set inverted by Sigloch et al (Nature Geosci, 2008), excluding only a small number of ISC delays at short distance and all amplitude data. The model is a cubed Earth model with 3,637,248 voxels spanning mantle and crust, with a resolution everywhere better than 70 km, to which 1912 event corrections are added. In each iteration we determine the optimal solution by a least squares inversion with minimal damping, after which we regularize the model in wavelet space. We then compute the residual data vector (after an intermediate scaling step), and solve for a model correction until a satisfactory chi-square fit for the truncated model is obtained. We present our final results on convergence as well as a

  4. Chemical Shuttle Additives in Lithium Ion Batteries

    SciTech Connect

    Patterson, Mary

    2013-03-31

    The goals of this program were to discover and implement a redox shuttle that is compatible with large format lithium ion cells utilizing LiNi{sub 1/3}Mn{sub 1/3}Co{sub 1/3}O{sub 2} (NMC) cathode material and to understand the mechanism of redox shuttle action. Many redox shuttles, both commercially available and experimental, were tested and much fundamental information regarding the mechanism of redox shuttle action was discovered. In particular, studies surrounding the mechanism of the reduction of the oxidized redox shuttle at the carbon anode surface were particularly revealing. The initial redox shuttle candidate, namely 2-(pentafluorophenyl)-tetrafluoro-1,3,2-benzodioxaborole (BDB) supplied by Argonne National Laboratory (ANL, Lemont, Illinois), did not effectively protect cells containing NMC cathodes from overcharge. The ANL-RS2 redox shuttle molecule, namely 1,4-bis(2-methoxyethoxy)-2,5-di-tert-butyl-benzene, which is a derivative of the commercially successful redox shuttle 2,5-di-tert-butyl-1,4-dimethoxybenzene (DDB, 3M, St. Paul, Minnesota), is an effective redox shuttle for cells employing LiFePO{sub 4} (LFP) cathode material. The main advantage of ANL-RS2 over DDB is its larger solubility in electrolyte; however, ANL-RS2 is not as stable as DDB. This shuttle also may be effectively used to rebalance cells in strings that utilize LFP cathodes. The shuttle is compatible with both LTO and graphite anode materials although the cell with graphite degrades faster than the cell with LTO, possibly because of a reaction with the SEI layer. The degradation products of redox shuttle ANL-RS2 were positively identified. Commercially available redox shuttles Li{sub 2}B{sub 12}F{sub 12} (Air Products, Allentown, Pennsylvania and Showa Denko, Japan) and DDB were evaluated and were found to be stable and effective redox shuttles at low C-rates. The Li{sub 2}B{sub 12}F{sub 12} is suitable for lithium ion cells utilizing a high voltage cathode (potential that is higher

  5. Space shuttle wheels and brakes

    NASA Technical Reports Server (NTRS)

    Carsley, R. B.

    1985-01-01

    The Space Shuttle Orbiter wheels were subjected to a combination of tests which are different than any previously conducted in the aerospace industry. The major testing difference is the computer generated dynamic landing profiles used during the certification process which subjected the wheels and tires to simulated landing loading conditions. The orbiter brakes use a unique combination of carbon composite linings and beryllium heat sink to minimize weight. The development of a new lining retention method was necessary in order to withstand the high temperature generated during the braking roll. As with many programs, the volume into which this hardware had to fit was established early in the program, with no provisions made for growth to offset the continuously increasing predicted orbiter landing weight.

  6. Space Shuttle - Now and later

    NASA Technical Reports Server (NTRS)

    Malkin, M. S.

    1979-01-01

    The Space Shuttle System consisting of four major elements, the orbiter, three main engines, an expendable external tank, and solid rocket boosters is examined. Dimensions, thrust and payload capacity are observed, noting cost reduction through recovery and subsequent reuse of some components. Return of the solid rocket boosters (SRB's) via parachute and recovery from the ocean for refurbishment is covered. Objectives for the first mission scheduled for November 1979 include achieving a 150 nautical mile orbit and carrying a payload consisting of development flight instrumentation (DFI), the induced environment contamination monitor (IECM), and the aerodynamic coefficients identification package (ACIP). A section devoted to performance enhancement, discusses weight reduction on the orbiter, external tanks and thrust augmentation through the use of strap on solid motors (SOSM).

  7. Microbial Check Valve for Shuttle

    NASA Technical Reports Server (NTRS)

    Colombo, G. V.; Putnam, D. F.; Sauer, R. L.

    1978-01-01

    The Microbial Check Valve (MCV) is a device developed for the Space Shuttle that prevents the transfer of viable microorganisms within water systems. The device is essentially a bed of resin material, impregnated with iodine, that kills microorganisms on contact. It prevents the cross-contamination of microorganisms from a nonpotable system into the potable water system when these systems are interconnected. In this regard, the function of the device is similar to that of the 'air gap' found in conventional one-gravity systems. Basic design data are presented including pressure drop, scaling factors, sizing criteria, and the results of challenging the device with suspensions of seven microorganisms including aerobes, anaerobes and spore formers.

  8. Shuttle Spacelab Core Equipment Freezer

    NASA Technical Reports Server (NTRS)

    Copeland, R. J.

    1977-01-01

    This paper describes the preliminary design of a Shuttle Spacelab Core Equipment Freezer. The unit will provide the capability to freeze and store many experiment specimens. Two models of the unit are planned. One model provides storage at -70 C; the other model will provide -70 C storage, a freeze dry capability, storage at a selectable temperature in the range of 0 C to -70 C, and means of maintaining close temperature tolerances. In addition an exchanger loop will be available at 4 C for cooling of a centrifuge and a remote storage compartment. A test tube holder, a dish holder and thermal capacitors for rapid freezing of large specimens will also be provided. A Stirling Cycle was selected as the active refrigerator for minimum cost and weight.

  9. Space shuttle main engine controller

    NASA Technical Reports Server (NTRS)

    Mattox, R. M.; White, J. B.

    1981-01-01

    A technical description of the space shuttle main engine controller, which provides engine checkout prior to launch, engine control and monitoring during launch, and engine safety and monitoring in orbit, is presented. Each of the major controller subassemblies, the central processing unit, the computer interface electronics, the input electronics, the output electronics, and the power supplies are described and discussed in detail along with engine and orbiter interfaces and operational requirements. The controller represents a unique application of digital concepts, techniques, and technology in monitoring, managing, and controlling a high performance rocket engine propulsion system. The operational requirements placed on the controller, the extremely harsh operating environment to which it is exposed, and the reliability demanded, result in the most complex and rugged digital system ever designed, fabricated, and flown.

  10. Space Shuttle Trace Gas Analyzer

    NASA Technical Reports Server (NTRS)

    Dencker, W.

    1975-01-01

    A Trace Gas Analyzer (TGA) with the ability to detect the presence of toxic contaminants in the Space Shuttle atmosphere within the subparts-per-million range is under development. The design is a modification of the miniaturized Gas Chromatograph-Mass Spectrometer (GCMS) developed for the Viking Mars Lander. An ambient air sample is injected onto the GC column from a constant volume sample loop and separated into individual compounds for identification by the MS. The GC-MS interface consists of an effluent divider and a silver-paladium separator, an electrochemical cell which removes more than 99.99% of the hydrogen carrier gas. The hydrogen is reclaimed and repressurized without affecting the separator efficiency, a feature which enables a considerable weight reduction in the carrier gas supply system.

  11. Shuttle Entry Air Data System

    NASA Technical Reports Server (NTRS)

    Siemers, P. M., III

    1978-01-01

    The SEADS system (Shuttle Entry Air Data System) is being developed to provide research quality hypersonic (M greater than 3.5) air data. SEADS will accomplish this through the instrumentation of the orbiter's baseline nose cap. The SEADS development program consists of (1) the design and testing program required to define a reinforced carbon-carbon (RCC) nose cap penetration concept which will not degrade nose cap performance, (2) the definition of analytical techniques and design criteria for array definition and flight data analysis, (3) the verification of these analytical techniques and array criteria through a comprehensive wind-tunnel test program, (4) the demonstration of the system concept through detailed testing, and (5) the analyses and tests required to flight-certify the SEADS system.

  12. Space Shuttle solid rocket booster

    NASA Technical Reports Server (NTRS)

    Hardy, G. B.

    1979-01-01

    Details of the design, operation, testing and recovery procedures of the reusable solid rocket boosters (SRB) are given. Using a composite PBAN propellant, they will provide the primary thrust (six million pounds maximum at 20 s after ignition) within a 3 g acceleration constraint, as well as thrust vector control for the Space Shuttle. The drogues were tested to a load of 305,000 pounds, and the main parachutes to 205,000. Insulation in the solid rocket motor (SRM) will be provided by asbestos-silica dioxide filled acrylonitrile butadiene rubber ('asbestos filled NBR') except in high erosion areas (principally in the aft dome), where a carbon-filled ethylene propylene diene monomer-neopreme rubber will be utilized. Furthermore, twenty uses for the SRM nozzle will be allowed by its ablative materials, which are principally carbon cloth and silica cloth phenolics.

  13. Shuttle atmospheric lidar research program

    NASA Technical Reports Server (NTRS)

    1979-01-01

    The Shuttle atmospheric lidar program is discussed in relation to an understanding of the processes governing the Earth's atmosphere and in the capacity to evaluate the atmospheric susceptibility to manmade and natural perturbations. Applications of the lidar which are discussed are the determination of the global flow of water vapor and pollutants in the troposphere, improvement of chemical and transport models of the stratosphere and mesosphere, evaluation of radiative models of the atmosphere, investigation of chemistry and transport of thermospheric atomic species, and investigation of magnetospheric aspects of sun/weather relationships. The features of the lidar measurements discussed are the high spatial resolution, control of the source wavelength and intensity, and high measurement specificity.

  14. Space Shuttle Orbiter descent navigation

    NASA Technical Reports Server (NTRS)

    Montez, M. N.; Madden, M. F.

    1982-01-01

    The entry operational sequence (OPS 3) begins approximately 2 hours prior to the deorbit maneuver and continues through atmospheric entry, terminal area energy management (TAEM), approach and landing, and rollout. During this flight phase, the navigation state vector is estimated by the Space Shuttle Orbiter onboard navigation system. This estimate is computed using a six-element sequential Kalman filter, which blends inertial measurement unit (IMU) delta-velocity data with external navaid data. The external navaids available to the filter are tactical air navigation (TACAN), barometric altimeter, and microwave scan beam landing system (MSBLS). Attention is given to the functional design of the Orbiter navigation system, the descent navigation sensors and measurement processing, predicted Kalman gains, correlation coefficients, and current flights navigation performance.

  15. Optimal control for electron shuttling

    NASA Astrophysics Data System (ADS)

    Zhang, Jun; Greenman, Loren; Deng, Xiaotian; Hayes, Ian M.; Whaley, K. Birgitta

    2013-06-01

    In this paper we apply an optimal control technique to derive control fields that transfer an electron between ends of a chain of donors or quantum dots. We formulate the transfer as an optimal steering problem, and then derive the dynamics of the optimal control. A numerical algorithm is developed to effectively generate control pulses. We apply this technique to transfer an electron between sites of a triple quantum dot and an ionized chain of phosphorus dopants in silicon. Using the optimal pulses for the spatial shuttling of phosphorus dopants, we then add hyperfine interactions to the Hamiltonian and show that a 500 G magnetic field will transfer the electron spatially as well as transferring the spin components of two of the four hyperfine states of the electron-nuclear spin pair.

  16. Space Shuttle Orbiter-Illustration

    NASA Technical Reports Server (NTRS)

    2001-01-01

    This illustration is an orbiter cutaway view with callouts. The orbiter is both the brains and heart of the Space Transportation System (STS). About the same size and weight as a DC-9 aircraft, the orbiter contains the pressurized crew compartment (which can normally carry up to seven crew members), the huge cargo bay, and the three main engines mounted on its aft end. There are three levels to the crew cabin. Uppermost is the flight deck where the commander and the pilot control the mission. The middeck is where the gallery, toilet, sleep stations, and storage and experiment lockers are found for the basic needs of weightless daily living. Also located in the middeck is the airlock hatch into the cargo bay and space beyond. It is through this hatch and airlock that astronauts go to don their spacesuits and marned maneuvering units in preparation for extravehicular activities, more popularly known as spacewalks. The Space Shuttle's cargo bay is adaptable to hundreds of tasks. Large enough to accommodate a tour bus (60 x 15 feet or 18.3 x 4.6 meters), the cargo bay carries satellites, spacecraft, and spacelab scientific laboratories to and from Earth orbit. It is also a work station for astronauts to repair satellites, a foundation from which to erect space structures, and a hold for retrieved satellites to be returned to Earth. Thermal tile insulation and blankets (also known as the thermal protection system or TPS) cover the underbelly, bottom of the wings, and other heat-bearing surfaces of the orbiter to protect it during its fiery reentry into the Earth's atmosphere. The Shuttle's 24,000 individual tiles are made primarily of pure-sand silicate fibers, mixed with a ceramic binder. The solid rocket boosters (SRB's) are designed as an in-house Marshall Space Flight Center project, with United Space Boosters as the assembly and refurbishment contractor. The solid rocket motor (SRM) is provided by the Morton Thiokol Corporation.

  17. Space Shuttle RTOS Bayesian Network

    NASA Technical Reports Server (NTRS)

    Morris, A. Terry; Beling, Peter A.

    2001-01-01

    With shrinking budgets and the requirements to increase reliability and operational life of the existing orbiter fleet, NASA has proposed various upgrades for the Space Shuttle that are consistent with national space policy. The cockpit avionics upgrade (CAU), a high priority item, has been selected as the next major upgrade. The primary functions of cockpit avionics include flight control, guidance and navigation, communication, and orbiter landing support. Secondary functions include the provision of operational services for non-avionics systems such as data handling for the payloads and caution and warning alerts to the crew. Recently, a process to selection the optimal commercial-off-the-shelf (COTS) real-time operating system (RTOS) for the CAU was conducted by United Space Alliance (USA) Corporation, which is a joint venture between Boeing and Lockheed Martin, the prime contractor for space shuttle operations. In order to independently assess the RTOS selection, NASA has used the Bayesian network-based scoring methodology described in this paper. Our two-stage methodology addresses the issue of RTOS acceptability by incorporating functional, performance and non-functional software measures related to reliability, interoperability, certifiability, efficiency, correctness, business, legal, product history, cost and life cycle. The first stage of the methodology involves obtaining scores for the various measures using a Bayesian network. The Bayesian network incorporates the causal relationships between the various and often competing measures of interest while also assisting the inherently complex decision analysis process with its ability to reason under uncertainty. The structure and selection of prior probabilities for the network is extracted from experts in the field of real-time operating systems. Scores for the various measures are computed using Bayesian probability. In the second stage, multi-criteria trade-off analyses are performed between the scores

  18. The Shuttle Radar Topography Mission

    NASA Astrophysics Data System (ADS)

    Farr, T. G.; Kobrick, M.

    2001-05-01

    The Shuttle Radar Topography Mission (SRTM), which flew successfully aboard Endeavour in February 2000, is a cooperative project between NASA and the National Imagery and Mapping Agency (NIMA). The mission was designed to use a single-pass radar interferometer to produce a digital elevation model of the Earth's land surface between about 60 degrees north and 56 degrees south latitude. The DEM will have 30 m horizontal resolution and about 15 m vertical errors. Two ortho-rectified C-band image mosaics are also planned. SRTM used a modification of the radar instrument that comprised the Spaceborne Radar Laboratory that flew twice on the Shuttle Endeavour in 1994. To collect the interferometric data, a 60 m mast, additional C-band antenna, and improved tracking and navigation devices were added. A second X-band antenna was also added by the German Space Agency, and produced higher resolution topographic measurements in strips nested within the full, C-band coverage. First results indicate that the radars and ancillary instruments worked very well. Data played back to the ground during the flight were processed to DEMs and products released hours after acquisition. An extensive program for calibration and verification of the SRTM data is now underway. When complete later this year, systematic processing of the data will begin, with final products emerging a continent at a time. Data processing will be completed by the end of 2002. Products will be transferred to the US Geological Survey's EROS Data Center for civilian archive and distribution. NIMA will handle Department of Defense distribution. * Work performed under contract to NASA.

  19. The Shuttle Radar Topography Mission

    NASA Astrophysics Data System (ADS)

    Farr, T. G.; Kobrick, M.

    2001-12-01

    The Shuttle Radar Topography Mission (SRTM), which flew successfully aboard Endeavour in February 2000, is a cooperative project between NASA, the National Imagery and Mapping Agency, and the German and Italian Space Agencies. The mission was designed to use a single-pass radar interferometer to produce a digital elevation model of the Earth's land surface between about 60 degrees north and 56 degrees south latitude. The DEM will have 30 m horizontal resolution and better than 15 m vertical errors. Two ortho-rectified C-band image mosaics are also planned. Data processing will be completed by the end of 2002. SRTM used a modification of the radar instrument that comprised the Spaceborne Radar Laboratory that flew twice on the Shuttle Endeavour in 1994. To collect the interferometric data, a 60 m mast, additional C-band antenna, and improved tracking and navigation devices were added. A second X-band antenna was also added by the German Space Agency, and produced higher resolution topographic measurements in strips nested within the full, C-band coverage. First results indicate that the radars and ancillary instruments worked very well. Data played back to the ground during the flight were processed to DEMs and products released hours after acquisition. An extensive program for calibration and verification of the SRTM data is now underway. When complete later this year, systematic processing of the data will begin, with final products emerging a continent at a time. Products will be transferred to the US Geological Survey's EROS Data Center for civilian archive and distribution. NIMA will handle Department of Defense distribution. * Work performed under contract to NASA.

  20. Space shuttle maintenance program planning document

    NASA Technical Reports Server (NTRS)

    Brown, D. V.

    1972-01-01

    A means for developing a space shuttle maintenance program which will be acceptable to the development centers, the operators (KSC and AF), and the manufacturer is presented. The general organization and decision processes for determining the essential scheduled maintenance requirements for the space shuttle orbiter are outlined. The development of initial scheduled maintenance programs is discussed. The remaining maintenance, that is non-scheduled or non-routine maintenance, is directed by the findings of the scheduled maintenance program and the normal operation of the shuttle. The remaining maintenance consists of maintenance actions to correct discrepancies noted during scheduled maintenance tasks, nonscheduled maintenance, normal operation, or condition monitoring.

  1. Quantum Mechanical Models Of The Fermi Shuttle

    SciTech Connect

    Sternberg, James

    2011-06-01

    The Fermi shuttle is a mechanism in which high energy electrons are produced in an atomic collision by multiple collisions with a target and a projectile atom. It is normally explained purely classically in terms of the electron's orbits prescribed in the collision. Common calculations to predict the Fermi shuttle use semi-classical methods, but these methods still rely on classical orbits. In reality such collisions belong to the realm of quantum mechanics, however. In this paper we discuss several purely quantum mechanical calculations which can produce the Fermi shuttle. Being quantum mechanical in nature, these calculations produce these features by wave interference, rather than by classical orbits.

  2. Shuttle Ku-band signal design study

    NASA Technical Reports Server (NTRS)

    Lindsey, W. C.; Braun, W. R.; Mckenzie, T. M.

    1978-01-01

    Carrier synchronization and data demodulation of Unbalanced Quadriphase Shift Keyed (UQPSK) Shuttle communications' signals by optimum and suboptimum methods are discussed. The problem of analyzing carrier reconstruction techniques for unbalanced QPSK signal formats is addressed. An evaluation of the demodulation approach of the Ku-Band Shuttle return link for UQPSK when the I-Q channel power ratio is large is carried out. The effects that Shuttle rocket motor plumes have on the RF communications are determined also. The effect of data asymmetry on bit error probability is discussed.

  3. The First Space Shuttle External Tank

    NASA Technical Reports Server (NTRS)

    1977-01-01

    The first Space Shuttle External Tank (ET), the Main Propulsion Test Article (MPTA), rolls off the assembly line on September 9, 1977 at Michoud Assembly Facility in New Orleans, Louisiana. The MPTA was then transported to the National Space Technology Laboratories (currently called Stennis Space Center) in southern Mississippi where it was used in the static test firing of the Shuttle's cluster of three main engines. Marshall Space Flight Center was responsible for developing the External Tank. External Tank contains two tanks, one for liquid hydrogen and one for liquid oxygen, and a plumbing system that supplies propellant to the Main Engines of the Space Shuttle Orbiter.

  4. Shuttle era waste management and biowaste monitoring

    NASA Technical Reports Server (NTRS)

    Sauer, R. L.; Fogal, G. L.

    1976-01-01

    The acquisition of crew biomedical data has been an important task on manned space missions. The monitoring of biowastes from the crew to support water and mineral balance studies and endocrine studies has been a valuable part of this activity. This paper will present a review of waste management systems used in past programs. This past experience will be cited as to its influence on the Shuttle design. Finally, the Shuttle baseline waste management system and the proposed Shuttle biomedical measurement and sampling systems will be presented.

  5. Analysis of sleep on Shuttle missions

    NASA Technical Reports Server (NTRS)

    Santy, Patricia A.; Kapanka, Heidi; Davis, Jeffrey R.; Stewart, Donald F.

    1988-01-01

    The sleep patterns of 58 Space Shuttle crew members are analyzed statistically on the basis of debriefing forms filled out within 3 days postflight. The data are compiled in a table, and photographs of typical sleep conditions on the Shuttle are provided. It is found that sleep disruption is relatively common on Shuttle missions, especially on the first and last days. Sleep medication was used by 19.4 percent of crew on single-shift flights and 50 percent of crew on dual-shift flights.

  6. Cooling radioisotope thermoelectric generators in the Shuttle

    NASA Technical Reports Server (NTRS)

    Norman, R. M.

    1978-01-01

    Radioisotope thermoelectric generators (RTG) to be used on future spacecraft and launched by the Shuttle must be cooled from the time they are installed and enclosed until the spacecraft is deployed from the Shuttle. A special Cooling Kit maintains their temperature well below critical by circulating water through the coils soldered to them and through a heat exchanger that boils water and externally discharges the resulting steam. The RTG Cooling Kit, including its support frame, if fully charged with about 64 kg of evaporation water, will increase the Shuttle launch mass by about 200 kg.

  7. Simulated lightning test shuttle .03 scale model. [(space shuttle orbiter)

    NASA Technical Reports Server (NTRS)

    Clifford, D. W.

    1974-01-01

    Lightning Attach Point tests were conducted for the space shuttle launch configuration (Orbiter, External Tank and Solid Rocket Boosters). A series of 250 long spark tests (15 to 20 foot sparks) determined that the orbiter may be struck on the nose, windshield brow, tail and wingtips during launch but not on the main engine nozzles which have been shown to be vulnerable to lightning damage. The orbiter main engine and SRB exhaust plumes were simulated electrically with physical models coated with graded resistance paints. The tests showed that the exhaust plumes from the SRB provide additional protection for the main engine nozzles. However, the tests showed that the Orbiter Thermal Protection System (TPS), which has also been shown to be vulnerable to lightning damage, may be struck during launch. Therefore further work is indicated in the areas of swept stroke studies on the model and on TPS panels. Further attach point testing is also indicated on the free-flying orbiter. Photographs of the test setup are shown.

  8. Photographer: KSC The 747 Shuttle Carrier Aircraft, carrying the Space Shuttle Orbiter Enterprise

    NASA Technical Reports Server (NTRS)

    1980-01-01

    Photographer: KSC The 747 Shuttle Carrier Aircraft, carrying the Space Shuttle Orbiter Enterprise piggyback, lifts off from the Shuttle Landing Facility's 15,000-foot-long runway at 11:03, August 10. Enterprise flown to KSC on April 10 for use in checking out assembly, test and launch facilities which will be used for the launch of its sister ship Columbia on the first Space Shuttle flight, will make a five-stop flight to NASA's Dryden Flight Research Center in California.

  9. Particulate contaminant relocation during shuttle ascent

    NASA Technical Reports Server (NTRS)

    Scialdone, J. J.

    1986-01-01

    The dislodgement, venting, and redeposition of particles on a surface in the shuttle bay by the vibroacoustic, gravitational, and aerodynamic forces present during shuttle ascent have been investigated. The particles of different sizes which are displaced, vented, and redistributed have been calculated; and an estimate of the increased number of particles on certain surfaces and the decrease on others has been indicated. The average sizes, velocities, and length of time for certain particles to leave the bay following initial shuttle doors opening and thermal tests have been calculated based on indirect data obtained during several shuttle flights. Suggestions for future measurements and observations to characterize the particulate environment and the techniques to limit the in-orbit particulate contamination of surfaces and environment have been offered.

  10. STS-43 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1991-01-01

    The STS-43 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem operations during the forty-second flight of the Space Shuttle Program and the ninth flight of the Orbiter Vehicle Atlantis (OV-104). In addition to the Atlantis vehicle, the flight vehicle consisted of the following: an External Tank (ET) designated as ET-47 (LWT-40); three Space Shuttle main engines (SSME's) (serial numbers 2024, 2012, and 2028 in positions 1, 2, and 3, respectively); and two Solid Rocket Boosters (SRB's) designated as BI-045. The primary objective of the STS-43 mission was to successfully deploy the Tracking and Data Relay Satellite-E/Inertial Upper Stage (TDRS-E/IUS) satellite and to perform all operations necessary to support the requirements of the Shuttle Solar Backscatter Ultraviolet (SSBUV) payload and the Space Station Heat Pipe Advanced Radiator Element (SHARE-2).

  11. Plasma waves associated with the space shuttle

    NASA Technical Reports Server (NTRS)

    Cairns, I. H.; Gurnett, D. A.

    1990-01-01

    Water molecules outgassed from the Space Shuttle suffer collisional charge-exchange with ionospheric oxygen ions, thereby forming unstable distributions of pick-up water ions and leading to high levels of plasma waves near the Shuttle. Liouville's equation with a charge-exchange source term is solved for the water ion distribution function as a function of position relative to the Shuttle. The observational characteristics of the near zone Shuttle waves are summarized. A linear theory in which beam like distributions of water ions drive Doppler shifted lower hybrid waves via the modified two stream instability is developed. This theory explains many characteristics of the near zone waves. Further work on the effects of wave nonlinearities and spatial inhomogeneity is required to explain the detailed frequency spectrum of the waves. The observed wave levels apparently satisfy the threshold condition for modulational instability of lower hybrid waves.

  12. Space Shuttle Main Engine Test Firing

    NASA Technical Reports Server (NTRS)

    1988-01-01

    A cloud of extremely hot steam boils out of the flame deflector at the A-1 test stand during a test firing of a Space Shuttle Main Engine (SSME) at the John C. Stennis Space Center, Hancock County, Mississippi.

  13. Shuttle mission simulator software conceptual design

    NASA Technical Reports Server (NTRS)

    Burke, J. F.

    1973-01-01

    Software conceptual designs (SCD) are presented for meeting the simulator requirements for the shuttle missions. The major areas of the SCD discussed include: malfunction insertion, flight software, applications software, systems software, and computer complex.

  14. The Representative Shuttle Environmental Control System

    NASA Technical Reports Server (NTRS)

    Brose, H. F.; Greenwood, F. H.; Thompson, C. D.; Willis, N. C.

    1974-01-01

    The Representative Shuttle Environmental Control System (RSECS) program was conceived to provide NASA with a prototype system representative of the Shuttle Environmental Control System (ECS). Discussed are the RSECS program objectives, predicated on updating and adding to the early system as required to retain its usefulness during the Shuttle ECS development and qualification effort. Ultimately, RSECS will be replaced with a flight-designed system using either refurbished development or qualification equipment to provide NASA with a flight simulation capability during the Shuttle missions. The RSECS air revitalization subsystem and the waste management support subsystem are being tested. A water coolant subsystem and a freon coolant subsystem are in the development and planning phases.

  15. Evaluation philosophy for shuttle launched payloads

    NASA Technical Reports Server (NTRS)

    Heuser, R. E.

    1975-01-01

    Potential benefits of factory-to-pad testing constitute major cost savings and increase test effectiveness. Overall flight performance will be improved. The factory-to-pad approach is compatible with space shuttle processing and the large space telescope program.

  16. Launch of STS-66 Space Shuttle Atlantis

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The Space Shuttle Atlantis returns to work after a refurbishing and a two-year layoff, as liftoff for NASA's STS-66 occurs at noon (EDT), November 3, 1994. A 'fish-eye' lens was used to record the image.

  17. NASA nixes Centaur launches from shuttle

    NASA Astrophysics Data System (ADS)

    Katzoff, Judith A.

    James C. Fletcher, the administrator of the National Aeronautics and Space Administration (NASA) announced on June 19, 1986, that because of safety considerations, the space shuttle will not be used to launch the Centaur Upper Stage. The Ulysses and Galileo missions, which were originally to have been launched in May 1986, would have been launched from the shuttle with the Centaur rocket (Eos, November 19, 1985, p. 1183; February 4, 1986, p. 57). The Galileo craft is to explore Jupiter; Ulysses is a joint mission of the European Space Agency (ESA) and NASA that is to orbit the sun around its poles, outside of the “ecliptic plane” where the planets lie. The decision seems likely to delay further the two missions, which were already delayed by the suspension of shuttle launches after the explosion of the space shuttle Challenger on January 28, 1986.

  18. Shuttle Main Engine Firing in Gimbal Test

    NASA Technical Reports Server (NTRS)

    1995-01-01

    A close-up view of a Space Shuttle Main Engine during a test at the John C. Stennis Space Center shows how the engine is gimballed, or rotated to evaluate the performance of its components under simulated flight conditions.

  19. A Lifeline Home: Goddard's Final Shuttle Mission

    NASA Video Gallery

    Controllers at Goddard's Network Integration Center share their thoughts as the 30-year-old Shuttle Program comes to an end with the final flight of STS-135, which concluded with a textbook landing...

  20. Student Experiments Fly with the Shuttle.

    ERIC Educational Resources Information Center

    Saunders, Walter; And Others

    1979-01-01

    Describes various experiments which high school students are preparing, to be carried on NASA's 500 or more Space Shuttle flights in the 1980s. The project is intended to stimulate superior secondary school students. (SA)

  1. Behind the Scenes: Under the Shuttle

    NASA Video Gallery

    In this episode of "NASA Behind the Scenes," astronaut Mike Massimino takes you up to - and under - the space shuttle as it waits on launch pad 39A at the Kennedy Space Center for the start of a re...

  2. Sturckow Recaps Last Shuttle Landing at Edwards

    NASA Video Gallery

    When Space Shuttle Discovery touched down at Edwards Air Force Base in Southern California on Sept. 11, 2009 to conclude mission STS-128, no one foresaw that it would be the last of 54 such landing...

  3. Space shuttle phase B study plan

    NASA Technical Reports Server (NTRS)

    Hello, B.

    1971-01-01

    Phase B emphasis was directed toward development of data which would facilitate selection of the booster concept, and main propulsion system for the orbiter. A shuttle system is also defined which will form the baseline for Phase C program activities.

  4. Space Shuttle Atlantis after RSS rollback

    NASA Technical Reports Server (NTRS)

    2001-01-01

    On Launch Pad 39A, the Rotating Service Structure has rolled back to reveal Space Shuttle Atlantis poised for launch. Atlantis is carrying the U.S. Laboratory Destiny, a key module in the growth of the International Space Station. Destiny will be attached to the Unity node on the Space Station using the Shuttle's robotic arm. Three spacewalks are required to complete the planned construction work during the 11-day mission. Launch is targeted for 6:11 p.m. EST and the planned landing at KSC Feb. 18 about 1:39 p.m. This mission marks the seventh Shuttle flight to the Space Station, the 23rd flight of Atlantis and the 102nd flight overall in NASA's Space Shuttle program.

  5. Shuttle Hitchhiker Experiment Launcher System (SHELS)

    NASA Technical Reports Server (NTRS)

    Daelemans, Gerry

    1999-01-01

    NASA's Goddard Space Flight Center Shuttle Small Payloads Project (SSPP), in partnership with the United States Air Force and NASA's Explorer Program, is developing a Shuttle based launch system called SHELS (Shuttle Hitchhiker Experiment Launcher System), which shall be capable of launching up to a 400 pound spacecraft from the Shuttle cargo bay. SHELS consists of a Marman band clamp push-plate ejection system mounted to a launch structure; the launch structure is mounted to one Orbiter sidewall adapter beam. Avionics mounted to the adapter beam will interface with Orbiter electrical services and provide optional umbilical services and ejection circuitry. SHELS provides an array of manifesting possibilities to a wide range of satellites.

  6. Magnetically controlled single-electron shuttle

    NASA Astrophysics Data System (ADS)

    Ilinskaya, O. A.; Kulinich, S. I.; Krive, I. V.; Shekhter, R. I.; Jonson, M.

    2015-01-01

    A theory of single-electron shuttling in an external magnetic field in nanoelectromechanical system with magnetic leads is presented. We consider partially spin-polarized electrons in the leads and electron transport in both the Coulomb blockade regime and in the limit of large bias voltages when the Coulomb blockade is lifted. The influence of the degree of spin polarization on shuttle instability is considered. It is shown that there is certain degree of spin polarization above which the magnetic field ceases to control electron transport. In the Coulomb blockade regime the dependence of the threshold magnetic field, which separates the "shuttle" and vibron regimes, on the degree of polarization is evaluated. The possibility of re-entrant transitions to the shuttle phase is discussed.

  7. The space shuttle program: a policy failure?

    PubMed

    Logsdon, J M

    1986-05-30

    The 5 January 1972 announcement by President Richard Nixon that the United States would develop during the 1970's a new space transportation system-the space shuttle-has had fundamental impacts on the character of U.S. space activities. In retrospect, it can be argued that the shuttle design chosen was destined to fail to meet many of the policy objectives established for the system; the shuttle's problems in serving as the primary launch vehicle for the United States and in providing routine and cost-effective space transportation are in large part a result of the ways in which compromises were made in the 1971-72 period in order to gain White House and congressional approval to proceed with the program. The decision to develop a space shuttle is an example of a poor quality national commitment to a major technological undertaking. PMID:17754497

  8. NASA's Original Shuttle Carrier Departs Dryden

    NASA Video Gallery

    NASA's Space Shuttle Carrier Aircraft (SCA) No. 905, departed NASA's Dryden Flight Research Center on Oct. 24, 2012 for the final time, ending a 38-year association with the NASA field center at Ed...

  9. A Celebration of the Space Shuttle Program

    NASA Video Gallery

    On September 23, 2011, NASA Langley hosted a Shuttle Celebration at the Virginia Air & Space Center in Hampton, Va. More than 650 guests attended, including STS-135 Commander Chris Ferguson and NAS...

  10. Astronauts Train for Final Shuttle Mission

    NASA Video Gallery

    The crew of STS-135, the final space shuttle mission, rehearsed their launch day process at NASA's Kennedy Space Center in Florida during a Terminal Countdown Demonstration Test that took place Jun...

  11. Shuttle Small Payloads Project Office: Overview

    NASA Technical Reports Server (NTRS)

    Dunker, S. Chris

    1999-01-01

    This viewgraph presentation gives an overview of the Shuttle Small Payload Project Office activities. Information is given on the Hitchhiker and Hitchhiker-Junior programs, the Get Away Special program, space experiment module program, program costs, and future enhancements.

  12. Space Shuttle Documentary (Narrated by William Shatner)

    NASA Video Gallery

    This feature-length documentary looks at the history of the most complex machine ever built. For 30 years, NASA's space shuttle carried humans to and from space, launched amazing observatories, and...

  13. Legacy of the Space Shuttle Program

    NASA Technical Reports Server (NTRS)

    Sullivan, Steven J.

    2010-01-01

    This slide presentation reviews many of the innovations from Kennedy Space Center engineering for ground operations that were made during the shuttle program. The innovations are in the areas of detection, image analysis, protective equipment, software development and communications.

  14. Composite reinforced propellant tanks. [space shuttles

    NASA Technical Reports Server (NTRS)

    Brown, L. D.; Martin, M. J.; Aleck, B. J.; Landes, R.

    1975-01-01

    Design studies involving weight and cost were carried out for several structural concepts applicable to space shuttle disposable tankage. An effective design, a honeycomb stabilized pressure vessel, was chosen. A test model was designed and fabricated.

  15. Space transportation system shuttle turnabout analysis report

    NASA Technical Reports Server (NTRS)

    Reedy, R. E.

    1979-01-01

    The progress made and the problems encountered by the various program elements of the shuttle program in achieving the 160 hour ground turnaround goal are presented and evaluated. Task assessment time is measured against the program allocation time.

  16. The Shuttle Cost and Price model

    NASA Technical Reports Server (NTRS)

    Leary, Katherine; Stone, Barbara

    1983-01-01

    The Shuttle Cost and Price (SCP) model was developed as a tool to assist in evaluating major aspects of Shuttle operations that have direct and indirect economic consequences. It incorporates the major aspects of NASA Pricing Policy and corresponds to the NASA definition of STS operating costs. An overview of the SCP model is presented and the cost model portion of SCP is described in detail. Selected recent applications of the SCP model to NASA Pricing Policy issues are presented.

  17. Computed Flow About The Integrated Space Shuttle

    NASA Technical Reports Server (NTRS)

    Buning, P. G.; Chiu, I. T.; Obayashi, S.; Rizk, Y. M.; Steger, J. L.

    1991-01-01

    Report discusses numerical simulations of flow of air about integrated Space Shuttle in ascent. Goal: to improve understanding of, and ability to predict, how integrated Space Shuttle will perform during both nominal and aborted ascent under various conditions. These and other numerical simulations intended both to supplement wind-tunnel data, corrupted to some extent by scaling and wall-interference effects, and to compensate for scarcity of valid flight data.

  18. Camcorders in Space Shuttle earth observations

    NASA Technical Reports Server (NTRS)

    Lulla, Kamlesh; Helfert, Michael

    1990-01-01

    A project to evaluate the use of commercially available camcorder systems during Shuttle flights is discussed, focusing on the use of an 8-mm camcorder for earth observations during the STS-30 mission in May, 1989. The camcorder with a 2/3-inch CCD is described, noting the modifications to prepare the camcorder for use on the Shuttle. The results of the camcorder project are summarized, listing the types of earth-viewing video images that were aquired with the camcorder.

  19. The 1995 Shuttle Small Payloads Symposium

    SciTech Connect

    Goldsmith, F.; Mosier, F.L.

    1995-09-01

    The 1995 Shuttle Small Payloads Symposium is a combined symposia of the Get Away Special (GAS) and Hitchhiker programs, and is proposed to continue as an annual conference. The focus of this conference is to educate potential Space Shuttle Payload Bay users as to the types of carrier systems provided and for current users to share experiment concepts. Separate abstracts have been submitted for contributions to this report.

  20. The 1993 Shuttle Small Payloads Symposium

    SciTech Connect

    Thomas, L.R.; Mosier, F.L.

    1993-10-01

    The 1993 Shuttle Small Payloads Symposium is a combined symposia of the Get Away Special (GAS), Hitchhiker, and Complex Autonomous Payloads (CAP) programs, and is proposed to continue as an annual conference. The focus of this conference is to educate potential Space Shuttle Payload Bay users as to the types of carrier systems provided and for current users to share experiment concepts. Separate abstracts have been prepared for articles from this report.

  1. The 1992 Shuttle Small Payloads Symposium

    SciTech Connect

    Thomas, L.R.; Mosier, F.L.

    1992-10-01

    The 1992 Shuttle Small Payloads Symposium is a continuation of the Get Away Special Symposium convened from 1984 through 1988, and is proposed to continue as an annual conference. The focus of this conference is to educate potential Space Shuttle Payload Bay users as to the types of carrier systems provided and for current users to share experiment concepts. Separate abstracts have been prepared for papers in this report.

  2. Probabilistic Approaches for Evaluating Space Shuttle Risks

    NASA Technical Reports Server (NTRS)

    Vesely, William

    2001-01-01

    The objectives of the Space Shuttle PRA (Probabilistic Risk Assessment) are to: (1) evaluate mission risks; (2) evaluate uncertainties and sensitivities; (3) prioritize contributors; (4) evaluate upgrades; (5) track risks; and (6) provide decision tools. This report discusses the significance of a Space Shuttle PRA and its participants. The elements and type of losses to be included are discussed. The program and probabilistic approaches are then discussed.

  3. Space Shuttle Program Tin Whisker Mitigation

    NASA Technical Reports Server (NTRS)

    Nishimi, Keith

    2007-01-01

    The discovery of tin whiskers (TW) on space shuttle hardware led to a program to investigate and removal and mitigation of the source of the tin whiskers. A Flight Control System (FCS) avionics box failed during vehicle testing, and was routed to the NASA Shuttle Logistics Depot for testing and disassembly. The internal inspection of the box revealed TW growth visible without magnification. The results of the Tiger Team that was assembled to investigate and develop recommendations are reviewed in this viewgraph presentation.

  4. Dynamic analysis for shuttle design verification

    NASA Technical Reports Server (NTRS)

    Fralich, R. W.; Green, C. E.; Rheinfurth, M. H.

    1972-01-01

    Two approaches that are used for determining the modes and frequencies of space shuttle structures are discussed. The first method, direct numerical analysis, involves finite element mathematical modeling of the space shuttle structure in order to use computer programs for dynamic structural analysis. The second method utilizes modal-coupling techniques of experimental verification made by vibrating only spacecraft components and by deducing modes and frequencies of the complete vehicle from results obtained in the component tests.

  5. Shuttle time and frequency transfer experiment

    NASA Technical Reports Server (NTRS)

    Allan, D. W.; Alley, C. O.; Decher, R.; Vessot, R. F. C.; Winkler, G. M. R.

    1980-01-01

    A proposed space shuttle experiment to demonstrate techniques for global high precision comparison of clocks and primary frequency standards is described. The experiment, using transmitted microwave and pulsed laser signals, compared a hydrogen maser clock onboard the space shuttle with a clock in a ground station in order to demonstrate time transfer with accuracies of 1 nsec or better and frequency comparison at the 10 to the -14th power accuracy level.

  6. Space Shuttle: The Renewed Promise

    NASA Technical Reports Server (NTRS)

    McAleer, Neil

    1989-01-01

    NASA celebrated its 30th anniversary in 1988, two days after the Space Shuttle soared into space once more. When Congress approved the creation of the National Aeronautics and Space Administration in 1958, the United States had successfully launched only four small satellites and no American astronaut had yet flown in space. In the three decades since, four generations of manned spacecraft have been built and flown, twelve men have walked on the Moon, more than 100 Americans have flown and worked in space, and communications satellites and other Space-Age technologies have transformed life on planet Earth. When NASA's Golden Anniversary is celebrated in 2008, it is likely that men and women will be permanently living and working in space. There may be a base on the Moon, and a manned mission to Mars may only be years away. If a brief history of the first half-century of the Space Age is written for that event, it will show clearly how the exploration of space has altered the course of human history and allowed us to take a better hold of our destiny on and off planet Earth.

  7. Shuttle Upgrade Program: Tile TPS

    NASA Technical Reports Server (NTRS)

    Leiser, Daniel B.; Stewart, David A.; DiFiore, Robert; Irby, Ed; Arnold, James (Technical Monitor)

    2001-01-01

    One of the areas where the thermal protection system on the Space Shuttle Orbiter could be improved is the RSI (Reusable Surface Insulation) tile. The improvement would be in damage resistance that would reduce the resultant maintenance and inspection required. It has performed very well in every other aspect. Improving the system's damage resistance has been the subject of much research over the past several years. One of the results of that research was a new system developed for damage prone areas on the orbiter (i.e., base heat shield). That system, designated as TUFI, Toughened Uni-Piece Fibrous Insulation, was successfully demonstrated as an experiment on the Orbiter and is now baselined for the base heat shield. This paper describes the results of a current research program to further improve the TUFI tile system, thus making it applicable to more areas on the orbiter. The way to remove the current limitations of the TUFI system (i.e., weight or thermal conductivity differences between it and the baseline tile (LI-900)) is to improve the characteristics of LI-900 or AETB-8. Specifically this paper describes the results of two efforts. The first shows performance data of an improved LI-900 system involving the application of TUFI and the second describes data that shows a reduced difference in thermal conductivity between the advanced TUFI substrate (AETB-8) now used on the orbiter and LI-900.

  8. Navigation of the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Edwards, A., Jr.

    1983-01-01

    Navigational systems and operations for the Space Shuttle are described. All navigational instrumentation is controlled from within the pressurized main cabin. Measurements of the state vector and the attitude are made with an inertial measurement unit (IMU), which uses data initialized at the moment of take-off. Orbital location is calculated in approximations using the initial propulsion conditions, models of the gravity field, and aerodynamic drag forces. Updates are periodically received from ground tracking stations. IMU continues attitude information, and additional references are made with an automated startracker device. Information can also be gathered by optical alignment, and future systems will include radar tracking in an approach mode. Deorbit is accompanied by IMU altitude measurements as well as calculations of altitude based on drag measurements. Barometric measurements begin at about 80,000 ft altitude. Signals are received from TACAN beginning at 145,000 ft, and the microwave scanning beam landing system is started at 20,000 ft. Various radionavigation systems are also employed in all flight phases.

  9. Space shuttle flying qualities and criteria assessment

    NASA Technical Reports Server (NTRS)

    Myers, T. T.; Johnston, D. E.; Mcruer, Duane T.

    1987-01-01

    Work accomplished under a series of study tasks for the Flying Qualities and Flight Control Systems Design Criteria Experiment (OFQ) of the Shuttle Orbiter Experiments Program (OEX) is summarized. The tasks involved review of applicability of existing flying quality and flight control system specification and criteria for the Shuttle; identification of potentially crucial flying quality deficiencies; dynamic modeling of the Shuttle Orbiter pilot/vehicle system in the terminal flight phases; devising a nonintrusive experimental program for extraction and identification of vehicle dynamics, pilot control strategy, and approach and landing performance metrics, and preparation of an OEX approach to produce a data archive and optimize use of the data to develop flying qualities for future space shuttle craft in general. Analytic modeling of the Orbiter's unconventional closed-loop dynamics in landing, modeling pilot control strategies, verification of vehicle dynamics and pilot control strategy from flight data, review of various existent or proposed aircraft flying quality parameters and criteria in comparison with the unique dynamic characteristics and control aspects of the Shuttle in landing; and finally a summary of conclusions and recommendations for developing flying quality criteria and design guides for future Shuttle craft.

  10. Shuttle delays squeeze launches of science missions

    NASA Astrophysics Data System (ADS)

    Like pinholes in a balloon, recent delays in preparations for the National Aeronautics and Space Administration's space shuttle Discovery are bleeding away enthusiasm and deflating hopes for an early September launch and resumption of the shuttle program after 2 years of soul-searching and cautious rebuilding. Official projections are now for an October lift-off. Further delays could threaten the success of many long-awaited scientific missions to be launched from the shuttle over the next year.A hydrogen leak was discovered July 29 during fueling of the shuttle at Cape Canaveral, Fla., and another fuel leak was discovered August 1. Shuttle engineers thought Discovery would have to be moved back to its hangar to repair the original leak, stalling the launch until at least November, but technicians were able to fix those leaks on the pad. However, a crucial test-firing of the three main shuttle engines August 4 was halted 5 seconds from ignition when one engine failed to fire because of a valve problem. A NASA press officer said the problem would be fixed on the pad and the test would probably occur within a week.

  11. Shuttle Carrier Aircraft (SCA) Fleet Photo

    NASA Technical Reports Server (NTRS)

    1995-01-01

    NASA's two Boeing 747 Shuttle Carrier Aircraft (SCA) are seen here nose to nose at Dryden Flight Research Center, Edwards, California. The front mounting attachment for the Shuttle can just be seen on top of each. The SCAs are used to ferry Space Shuttle orbiters from landing sites back to the launch complex at the Kennedy Space Center, and also to and from other locations too distant for the orbiters to be delivered by ground transportation. The orbiters are placed atop the SCAs by Mate-Demate Devices, large gantry-like structures which hoist the orbiters off the ground for post-flight servicing, and then mate them with the SCAs for ferry flights. Features which distinguish the two SCAs from standard 747 jetliners are; three struts, with associated interior structural strengthening, protruding from the top of the fuselage (two aft, one forward) on which the orbiter is attached, and two additional vertical stabilizers, one on each end of the standard horizontal stabilizer, to enhance directional stability. The two SCAs are under the operational control of NASA's Johnson Space Center, Houston, Texas. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back

  12. NESTA: NASA Engineering Shuttle Telemetry Agent

    NASA Technical Reports Server (NTRS)

    Semmel, Glenn S.; Davis, Steven R.; Leucht, Kurt W.; Rowe, Dan A.; Smith, Kevin E.; Boloni, Ladislau

    2005-01-01

    The Spaceport Processing Systems Branch at NASA Kennedy Space Center has developed and deployed an agent based tool to monitor the Space Shuttle's ground processing telemetry stream. The application, the NASA Engineering Shuttle Telemetry Agent, increases situational awareness for system and hardware engineers during ground processing of the Shuttle's subsystems. The agent provides autonomous monitoring of the telemetry stream and automatically alerts system engineers when predefined criteria have been met. Efficiency and safety are improved through increased automation. Sandia National Labs' Java Expert System Shell is employed as the rule engine. The shell's predicate logic lends itself well to capturing the heuristics and specifying the engineering rules of this spaceport domain. The declarative paradigm of the rule-based agent yields a highly modular and scalable design spanning multiple subsystems of the Shuttle. Several hundred monitoring rules have been written thus far with corresponding notifications sent to Shuttle engineers. This paper discusses the rule-based telemetry agent used for Space Shuttle ground processing and explains the problem domain, development of the agent software, benefits of AT technology, and deployment and sustaining engineering of the product.

  13. Designing the Space Shuttle Propulsion System

    NASA Technical Reports Server (NTRS)

    Owen, James; Moore, Dennis; Wood, David; VanHooser, Kathrine; Wlzyn, Ken

    2011-01-01

    The major elements of the Space Shuttle Main Propulsion System include two reusable solid rocket motors integrated into recoverable solid rocket boosters, an expendable external fuel and oxidizer tank, and three reusable Space Shuttle Main Engines. Both the solid rocket motors and space shuttle main engines ignite prior to liftoff, with the solid rocket boosters separating about two minutes into flight. The external tank separates after main engine shutdown and is safely expended in the ocean. The SSME's, integrated into the Space Shuttle Orbiter aft structure, are reused after post landing inspections. Both the solid rocket motors and the space shuttle main engine throttle during early ascent flight to limit aerodynamic loads on the structure. The configuration is called a stage and a half as all the propulsion elements are active during the boost phase, and the SSME's continue operation to achieve orbital velocity approximately eight and a half minutes after liftoff. Design and performance challenges were numerous, beginning with development work in the 1970 s. The solid rocket motors were large, and this technology had never been used for human space flight. The SSME s were both reusable and very high performance staged combustion cycle engines, also unique to the Space Shuttle. The multi body side mount configuration was unique and posed numerous integration and interface challenges across the elements. Operation of the system was complex and time consuming. This paper discusses a number of the system level technical challenges including development and operations.

  14. Simulating Avionics Upgrades to the Space Shuttles

    NASA Technical Reports Server (NTRS)

    Deger, Daniel; Hill, Kenneth; Braaten, Karsten E.

    2008-01-01

    Cockpit Avionics Prototyping Environment (CAPE) is a computer program that simulates the functions of proposed upgraded avionics for a space shuttle. In CAPE, pre-existing space-shuttle-simulation programs are merged with a commercial-off-the-shelf (COTS) display-development program, yielding a package of software that enables high-fi46 NASA Tech Briefs, September 2008 delity simulation while making it possible to rapidly change avionic displays and the underlying model algorithms. The pre-existing simulation programs are Shuttle Engineering Simulation, Shuttle Engineering Simulation II, Interactive Control and Docking Simulation, and Shuttle Mission Simulator playback. The COTS program Virtual Application Prototyping System (VAPS) not only enables the development of displays but also makes it possible to move data about, capture and process events, and connect to a simulation. VAPS also enables the user to write code in the C or C++ programming language and compile that code into the end-product simulation software. As many as ten different avionic-upgrade ideas can be incorporated in a single compilation and, thus, tested in a single simulation run. CAPE can be run in conjunction with any or all of four simulations, each representing a different phase of a space-shuttle flight.

  15. TERSSE. Definition of the total earth resources system for the shuttle era. Volume 9: Earth resources shuttle applications

    NASA Technical Reports Server (NTRS)

    Alverado, U.

    1975-01-01

    The use of the space shuttle for the Earth Resources Program is discussed. Several problems with respect to payload selection, integration, and mission planning were studied. Each of four shuttle roles in the sortie mode were examined and projected into an integrated shuttle program. Several representative Earth Resources missions were designed which would use the shuttle sortie as a platform and collectively include the four shuttle roles. An integrated flight program based on these missions was then developed for the first two years of shuttle flights. A set of broad implications concerning the uses of the shuttle for Earth Resources studies resulted.

  16. Success Legacy of the Space Shuttle Program: Changes in Shuttle Post Challenger and Columbia

    NASA Technical Reports Server (NTRS)

    Jarrell, George

    2010-01-01

    This slide presentation reviews the legacy of successes in the space shuttle program particularly with regards to the changes in the culture of NASA's organization after the Challenger and Columbia accidents and some of the changes to the shuttles that were made manifest as a result of the accidents..

  17. Infrared spectral measurement of space shuttle glow

    SciTech Connect

    Ahmadijian, M.

    1992-01-01

    Infrared spectral measurements of the space shuttle glow were successfully conducted during the STS-39 space shuttle mission. Analysis indicates that NO, NO[sup +], OH, and CO are among the molecules associated with the infrared glow phenomenon. During orbiter thruster firings the glow intensities in the infrared are enhanced by factors of 10x to 100x with significant changes in spectral distribution. These measurements were obtained with the Spacecraft Kinetic Infrared Test (SKIRT) payload which included a cryogenic infrared circular variable filter (CVF) spectrometer (0.6 [mu]m to 5.4 [mu]) and a number of infrared, visible, and ultraviolet radiometers (0.2 [mu]m to 5.4 [mu]m and 9.9 [mu]m to 10.4 [mu]m). In addition, glow measurements were unsuccessfully attempted with the Cryogenic Infrared Radiance Instrumentation for Shuttle (CIRRIS-1A) with its 2.5 [mu]m to 25 [mu]m Fourier transform interferometer. SKIRT CVF obtained over 14,000 spectra of quiescent shuttle glow, thruster enhanced shuttle glow, upper atmosphere airglow, aurora, orbiter environment, and deep space non-glow backgrounds during its eight day mission. The SKIRT radiometers operated almost continuously throughout the mission to provide a detailed history of the IR/VIS/UV optical environment associated with the operation of large spacecraft structures in low earth orbit. This dissertation will primarily address those measurements conducted by the SKIRT spectrometer as they relate to space shuttle glow in the infrared. The STS-39 Space Shuttle Discovery was launched from the NASA Kennedy Space Center on 28 April 1991 into a 57 degree inclination circular orbit at an altitude of 260 km.

  18. STS-31 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Camp, David W.; Germany, D. M.; Nicholson, Leonard S.

    1990-01-01

    The STS-31 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities on this thirty-fifth flight of the Space Shuttle and the tenth flight of the Orbiter Vehicle Discovery (OV-103). In addition to the Discovery vehicle, the flight vehicle consisted of an External Tank (ET) (designated as ET-34/LWT-27), three Space Shuttle main engines (SSME's) (serial numbers 2011, 2031, and 2107), and two Solid Rocket Booster (SRB) (designated as BI-037). The primary objective of the mission was to place the Hubble Space Telescope (HST) into a 330 nmi. circular orbit having an inclination of 28.45 degrees. The secondary objectives were to perform all operations necessary to support the requirements of the Protein Crystal Growth (PCG), Investigations into Polymer Membrane Processing (IPMP), Radiation Monitoring Equipment (RME), Ascent Particle Monitor (APM), IMAX Cargo Bay Camera (ICBC), Air Force Maui Optical Site Calibration Test (AMOS), IMAX Crew Compartment Camera, and Ion Arc payloads. In addition, 12 development test objectives (DTO's) and 10 detailed supplementary objectives (DSO's) were assigned to the flight. The sequence of events for this mission is shown. The significant problems that occurred in the Space Shuttle Orbiter subsystems during the mission are summarized, and the official problem tracking list is presented. In addition, each of the Space Shuttle Orbiter problems is cited in the subsystem discussion.

  19. STS-64 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1995-01-01

    The STS-64 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the sixty-fourth flight of the Space Shuttle Program and the nineteenth flight of the Orbiter vehicle Discovery (OV-103). In addition to the Orbiter, the flight vehicle consisted of an ET that was designated ET-66; three SSMEs that were designated as serial numbers 2031, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's that were designated Bl-068. The RSRM's that were installed in each SRB were designated as 360L041 A for the left SRB, and 360L041 B for the right SRB. The primary objective of this flight was to successfully perform the planned operations of the Lidar In-Space Technology Experiment (LITE), and to deploy the Shuttle Pointed Autonomous Research Tool for Astronomy (SPARTAN) -201 payload. The secondary objectives were to perform the planned activities of the Robot Operated Materials Processing System (ROMPS), the Shuttle Amateur Radio Experiment - 2 (SAREX-2), the Solid Surface Combustion Experiment (SSCE), the Biological Research in Canisters (BRIC) experiment, the Radiation Monitoring Equipment-3 (RME-3) payload, the Military Application of Ship Tracks (MAST) experiment, and the Air Force Maui Optical Site Calibration Test (AMOS) payload.

  20. Space Shuttle Atlantis after RSS rollback

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Lights on the Fixed Service Structure give a holiday impression at Launch Pad 39A where Space Shuttle Atlantis is poised for launch. Above the yellow-orange external tank is the Gaseous Oxygen Vent Arm, with the '''beanie cap''' vent hood raised. Before cryogenic loading, the hood will be lowered into position over the external tank vent louvers to vent gaseous oxygen vapors away from the Shuttle. Atlantis is carrying the U.S. Laboratory Destiny, a key module in the growth of the International Space Station. Destiny will be attached to the Unity node on the Space Station using the Shuttle's robotic arm. Three spacewalks are required to complete the planned construction work during the 11- day mission. Launch is targeted for 6:11 p.m. EST and the planned landing at KSC Feb. 18 about 1:39 p.m. This mission marks the seventh Shuttle flight to the Space Station, the 23rd flight of Atlantis and the 102nd flight overall in NASA's Space Shuttle program.

  1. Space Shuttle Solid Rocket Booster Debris Assessment

    NASA Technical Reports Server (NTRS)

    Kendall, Kristin; Kanner, Howard; Yu, Weiping

    2006-01-01

    The Space Shuttle Columbia Accident revealed a fundamental problem of the Space Shuttle Program regarding debris. Prior to the tragedy, the Space Shuttle requirement stated that no debris should be liberated that would jeopardize the flight crew and/or mission success. When the accident investigation determined that a large piece of foam debris was the primary cause of the loss of the shuttle and crew, it became apparent that the risk and scope of - damage that could be caused by certain types of debris, especially - ice and foam, were not fully understood. There was no clear understanding of the materials that could become debris, the path the debris might take during flight, the structures the debris might impact or the damage the impact might cause. In addition to supporting the primary NASA and USA goal of returning the Space Shuttle to flight by understanding the SRB debris environment and capability to withstand that environment, the SRB debris assessment project was divided into four primary tasks that were required to be completed to support the RTF goal. These tasks were (1) debris environment definition, (2) impact testing, (3) model correlation and (4) hardware evaluation. Additionally, the project aligned with USA's corporate goals of safety, customer satisfaction, professional development and fiscal accountability.

  2. STS-57 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1993-01-01

    The STS-57 Space Shuttle Program Mission Report provides a summary of the Payloads, as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the fifty-sixth flight of the Space Shuttle Program and fourth flight of the Orbiter vehicle Endeavour (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET (ET-58); three SSME's which were designated as serial numbers 2019, 2034, and 2017 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-059. The lightweight RSRM's that were installed in each SRB were designated as 360L032A for the left SRB and 360W032B for the right SRB. The STS-57 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement, as documented in NSTS 07700, Volume 8, Appendix E. That document states that each major organizational element supporting the Program will report the results of their hardware evaluation and mission performance plus identify all related in-flight anomalies.

  3. Space Shuttle Underside Astronaut Communications Performance Evaluation

    NASA Technical Reports Server (NTRS)

    Hwu, Shian U.; Dobbins, Justin A.; Loh, Yin-Chung; Kroll, Quin D.; Sham, Catherine C.

    2005-01-01

    The Space Shuttle Ultra High Frequency (UHF) communications system is planned to provide Radio Frequency (RF) coverage for astronauts working underside of the Space Shuttle Orbiter (SSO) for thermal tile inspection and repairing. This study is to assess the Space Shuttle UHF communication performance for astronauts in the shadow region without line-of-sight (LOS) to the Space Shuttle and Space Station UHF antennas. To insure the RF coverage performance at anticipated astronaut worksites, the link margin between the UHF antennas and Extravehicular Activity (EVA) Astronauts with significant vehicle structure blockage was analyzed. A series of near-field measurements were performed using the NASA/JSC Anechoic Chamber Antenna test facilities. Computational investigations were also performed using the electromagnetic modeling techniques. The computer simulation tool based on the Geometrical Theory of Diffraction (GTD) was used to compute the signal strengths. The signal strength was obtained by computing the reflected and diffracted fields along the propagation paths between the transmitting and receiving antennas. Based on the results obtained in this study, RF coverage for UHF communication links was determined for the anticipated astronaut worksite in the shadow region underneath the Space Shuttle.

  4. Shuttle Propulsion Overview - The Design Challenges

    NASA Technical Reports Server (NTRS)

    Owen, James W.

    2011-01-01

    The major elements of the Space Shuttle Main Propulsion System include two reusable solid rocket motors integrated into recoverable solid rocket boosters, an expendable external fuel and oxidizer tank, and three reusable Space Shuttle Main Engines. Both the solid rocket motors and space shuttle main engines ignite prior to liftoff, with the solid rocket boosters separating about two minutes into flight. The external tank separates, about eight and a half minutes into the flight, after main engine shutdown and is safely expended in the ocean. The SSME's, integrated into the Space Shuttle Orbiter aft structure, are reused after post landing inspections. The configuration is called a stage and a half as all the propulsion elements are active during the boost phase, with only the SSME s continuing operation to achieve orbital velocity. Design and performance challenges were numerous, beginning with development work in the 1970's. The solid rocket motors were large, and this technology had never been used for human space flight. The SSME s were both reusable and very high performance staged combustion cycle engines, also unique to the Space Shuttle. The multi body side mount configuration was unique and posed numerous integration and interface challenges across the elements. Operation of the system was complex and time consuming. This paper describes the design challenges and key areas where the design evolved during the program.

  5. Space Shuttle Navigation in the GPS Era

    NASA Technical Reports Server (NTRS)

    Goodman, John L.

    2001-01-01

    The Space Shuttle navigation architecture was originally designed in the 1970s. A variety of on-board and ground based navigation sensors and computers are used during the ascent, orbit coast, rendezvous, (including proximity operations and docking) and entry flight phases. With the advent of GPS navigation and tightly coupled GPS/INS Units employing strapdown sensors, opportunities to improve and streamline the Shuttle navigation process are being pursued. These improvements can potentially result in increased safety, reliability, and cost savings in maintenance through the replacement of older technologies and elimination of ground support systems (such as Tactical Air Control and Navigation (TACAN), Microwave Landing System (MLS) and ground radar). Selection and missionization of "off the shelf" GPS and GPS/INS units pose a unique challenge since the units in question were not originally designed for the Space Shuttle application. Various options for integrating GPS and GPS/INS units with the existing orbiter avionics system were considered in light of budget constraints, software quality concerns, and schedule limitations. An overview of Shuttle navigation methodology from 1981 to the present is given, along with how GPS and GPS/INS technology will change, or not change, the way Space Shuttle navigation is performed in the 21 5 century.

  6. Environmental protection requirements for scout/shuttle auxiliary stages

    NASA Technical Reports Server (NTRS)

    Qualls, G. L.; Kress, S. S.; Storey, W. W.; Ransdell, P. N.

    1980-01-01

    The requirements for enabling the Scout upper stages to endure the expected temperature, mechanical shock, acoustical and mechanical vibration environments during a specified shuttle mission were determined. The study consisted of: determining a shuttle mission trajectory for a 545 kilogram (1200 pound) Scout payload; compilation of shuttle environmental conditions; determining of Scout upper stages environments in shuttle missions; compilation of Scout upper stages environmental qualification criteria and comparison to shuttle mission expected environments; and recommendations for enabling Scout upper stages to endure the exptected shuttle mission environments.

  7. Space Shuttle Main Engine Debris Testing Methodology and Impact Tolerances

    NASA Technical Reports Server (NTRS)

    Gradl, Paul R.; Stephens, Walter

    2005-01-01

    In the wake of the Space Shuttle Columbia disaster every effort is being made to determine the susceptibility of Space Shuttle elements to debris impacts. Ice and frost debris is formed around the aft heat shield closure of the orbiter and liquid hydrogen feedlines. This debris has been observed to liberate upon lift-off of the shuttle and presents potentially dangerous conditions to the Space Shuttle Main Engine. This paper describes the testing done to determine the impact tolerance of the Space Shuttle Main Engine nozzle coolant tubes to ice strikes originating from the launch pad or other parts of the shuttle.

  8. STS-37 Shuttle Crew after Edwards landing

    NASA Technical Reports Server (NTRS)

    1991-01-01

    The crew of the Space Shuttle Atlantis gives the 'all's well' thumb's-up sign after leaving the 100-ton orbiter following their landing at 6:55 a.m. (PDT), 11 April 1991, at NASA's Ames Dryden Flight Research Facility (later redesignated Dryden Flight Research Center), Edwards, California, to conclude mission STS-37. They are, from left, Kenneth D. Cameron, pilot; Steven R. Nagel, mission commander; and mission specialists Linda M. Godwin, Jerry L. Ross, and Jay Apt. During the mission,which began with launch April 5 at Kennedy Space Center, Florida, the crew deployed the Gamma Ray Observatory. Ross and Jay also carried out two spacewalks, one to deploy an antenna on the Gamma Ray Observatory and the other to test equipment and mobility techniques for the construction of the future Space Station. The planned five-day mission was extended one day because of high winds at Edwards. Space Shuttles are the main element of America's Space Transportation System and are used for space research and other space applications. The shuttles are the first vehicles capable of being launched into space and returning to Earth on a routine basis. Space Shuttles are used as orbiting laboratories in which scientists and mission specialists conduct a wide variety of scientific experiments. Crews aboard shuttles place satellites in orbit, rendezvous with satellites to carry out repair missions and return them to space, and retrieve satellites and return them to Earth for refurbishment and reuse. Space Shuttles are true aerospace vehicles. They leave Earth and its atmosphere under rocket power provided by three liquid-propellant main engines with two solid-propellant boosters attached plus an external liquid-fuel tank. After their orbital missions, they streak back through the atmosphere and land like airplanes. The returning shuttles, however, land like gliders, without power and on runways. Other rockets can place heavy payloads into orbit, but, they can only be used once. Space

  9. STS-49: Space shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1992-01-01

    The STS-49 Space Shuttle Program Mission Report contains a summary of the Orbiter, External Tank (ET), Solid Rocket Booster/Redesigned Solid Rocket Motor (SRB/RSRM), and Space Shuttle main engine (SSME) subsystem performance during the forty-seventh flight of the Space Shuttle Program and the first flight of the Orbiter vehicle Endeavor (OV-105). In addition to the Endeavor vehicle, the flight vehicle consisted of an ET designated as ET-43 (LWT-36); three SSME's which were serial numbers 2030, 2015, and 2017 in positions 1, 2, and 3, respectively; and two SRB's designated as BI-050. The lightweight RSRM's installed in each SRB were designated as 360L022A for the left RSRM and 360L022B for the right RSRM.

  10. Space Shuttle payloads and data handling accommodations

    NASA Technical Reports Server (NTRS)

    Teasdale, W. E.

    1978-01-01

    The Space Transportation System, which includes the Space Shuttle, will reduce the costs of earth orbital operations while improving operational capabilities and flexibility. The Shuttle can deliver payloads up to 65,000 pounds to a 150-nmi circular orbit at a substantially lower operational cost per flight than the Titan III-C system, which has a payload delivery capability of only 30,000 pounds. In addition, the Space Shuttle can return to earth with up to 32,000 pounds of payload, a capability not provided by expendable launch vehicles. Typical payload missions are considered along with aspects of satellite placement and recovery, typical free-flying payloads, attached scientific payloads, and the typical nature of payload data.

  11. Space Shuttle Pinhole Formation Mechanism Studies

    NASA Technical Reports Server (NTRS)

    Jacobson, Nathan S.

    1998-01-01

    Pinholes have been observed to form on the wing leading edge of the space shuttle after about 10-15 flights. In this report we expand upon previous observations by Christensen (1) that these pinholes often form along cracks and are associated with a locally zinc-rich area. The zinc appears to come from weathering and peeling paint on the launch structure. Three types of experimental examinations are performed to understand this issue further: (A) Detailed microstructural examination of actual shuttle pinholes (B) Mass spectrometric studies of coupons containing, actual shuttle pinholes and (C) Laboratory furnace studies of ZnO/SiC reactions and ZnO/SiC protected carbon/carbon reaction. On basis of these observations we present a detailed mechanism of pinhole formation due to formation of a corrosive ZnO-Na-2-O-SiO2 ternary glass, which flows into existing cracks and enlarges them.

  12. STS-39 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1991-01-01

    The STS-39 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem operations during the fortieth flight of the Space Shuttle and the twelfth flight of the Orbiter Vehicle Discovery (OV-103). In addition to the Discovery vehicle, the flight vehicle consisted of the following: an External Tank (ET) (designated as ET-46 (LWT-39); three Space Shuttle main engines (SSME's) (serial numbers 2026, 2030, and 2029 in positions 1, 2, and 3, respectively); and two Solid Rocket Boosters (SRB's) designated as BI-043. The primary objective of this flight was to successfully perform the planned operations of the Infrared Background Signature Survey (IBSS), Air Force Payload (AFP)-675, Space Test Payload (STP)-1, and the Multipurpose Experiment Canister (MPEC) payloads.

  13. Shuttle Liquid Fly Back Booster Configuration Options

    NASA Technical Reports Server (NTRS)

    Healy, T. J., Jr.

    1998-01-01

    This paper surveys the basic configuration options available to a Liquid Fly Back Booster (LFBB), integrated with the Space Shuttle system. The background of the development of the LFBB concept is given. The influence of the main booster engine (BME) installations and the Fly Back Engine (FBE) installation on the aerodynamic configurations are also discussed. Limits on the LFBB configuration design space imposed by the existing Shuttle flight and ground elements are also described. The objective of the paper is to put the constrains and design space for an LFBB in perspective. The object of the work is to define LFBB configurations that significantly improve safety, operability, reliability and performance of the Shuttle system and dramatically lower operations costs.

  14. STS-48 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1991-01-01

    The STS-48 Space Shuttle Program Mission Report is a summary of the vehicle subsystem operations during the forty-third flight of the Space Shuttle Program and the thirteenth flight of the Orbiter vehicle Discovery (OV-103). In addition to the Discovery vehicle, the flight vehicle consisted of the following: an External Tank (ET) designated as ET-42 (LUT-35); three Space Shuttle main engines (SSME's) (serial numbers 2019, 2031, and 2107 in positions 1, 2, and 3, respectively); and two Solid Rocket Boosters (SRB's) designated as BI-046. The lightweight redesigned Solid Rocket Motors (RSRM's) installed in each one of the SRB's were designated as 360L018A for the left SRB and 360L018B for the right SRB. The primary objective of the flight was to successfully deploy the Upper Atmospheric Research Satellite (UARS) payload.

  15. Shuttle rendezvous radar performance evaluation and simulation

    NASA Technical Reports Server (NTRS)

    Griffin, John W.; Lindberg, Andrew C.; Ahn, Thomas B.; Harton, Paul L.

    1988-01-01

    The US Space Shuttle's Ku-band system was specifically designed for communications and tracking functions which are required during on-orbit operations with other spacecraft. Operating modes permit search and acquisition to be accomplished by computer designation or under manual control by the astronaut. Ku-band system data channels drive on-board dedicated displays and are incorporated into state vector updates by Shuttle guidance and navigation computers. Radar-cross-section estimates were used in computer simulations to predict the range at which radar detection and acquisition can be expected. Validity of the simulationi model and the radar design and performance were verified by flight tests on the White Sands test range. It is concluded that results of the testing established confidence in the capability of the system to provide the relative position and rate information which is needed for Shuttle work involving other spacecraft.

  16. STS-58 Space Shuttle Mission Report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1994-01-01

    The STS-58 Space Shuttle Program Mission Report provides a summary of the payload activities as well as the orbiter, external tank (ET), solid rocket booster (SRB) and redesigned solid rocket motor (RSRM), and the space shuttle main engine (SSME) subsystems performance during the fifty-eighth mission of the space shuttle program and fifteenth flight of the orbiter vehicle Columbia (OV-102). In addition to the orbiter, the flight vehicle consisted of an ET (ET-57); three SSME's, which were designated as serial numbers 2024, 2109, and 2018 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-061. The lightweight RSRM's that were installed in each SRB were designated as 360L034A for the left SRB and 360W034B for the right SRB.

  17. Ranger telerobotic shuttle experiment: a status report

    NASA Astrophysics Data System (ADS)

    Gefke, Gardell; Carignan, Craig R.; Roberts, Brian E.; Lane, J. Corde

    2002-02-01

    This paper presents an update on the Ranger Telerobotic Shuttle Experiment (RTSX) and associated key robotics technologies within the Ranger program. Ranger TSX will operate from a Spacelab logistics pallet inside the cargo bay of the shuttle and will demonstrate space station and on-orbit servicing operations including extravehicular (EVA) worksite setup, an orbital replacement unit (ORU) exchange, and various task board experiments. The flight system will be teleoperated from the middeck inside the shuttle as well as from a ground control station at NASA Johnson Space Center. This paper addresses the technical and programmatic status of the flight experiment and describes progress on the engineering test unit, Ranger Neutral Buoyancy Vehicle II (RNBVII), currently in fabrication. Also described are associated technologies, which support this effort. These include a flight robot mockup built to practice EVA stowage and Ranger NBV I, a free-flight prototype vehicle.

  18. Emittance measurements of RCG coated Shuttle tiles

    NASA Technical Reports Server (NTRS)

    Bouslog, Stanley A.; Cunnington, George R., Jr.

    1992-01-01

    The spectral and total normal emittance of the Reaction Cured Glass (RCG) coating used on Shuttle tiles has been measured for surface temperatures of 300 to 1905 K. These measurements were made on two virgin and two flown Shuttle tile samples. Room temperature directional emittance data were also obtained and used to determine the total hemispherical emittance of RCG as a function of temperature. The data obtained from this calculation indicate that the total hemispherical emittance decreases from a room temperature value of 0.83 to a value of 0.76 at 1905 K. The flown Shuttle tiles exhibited a change in the spectral distribution of emittance compared to that of the virgin tile, but no significant trends in the total emittance from a virgin to a flown tile could be established.

  19. Coulomb blockade of spin-dependent shuttling

    NASA Astrophysics Data System (ADS)

    Park, Hee Chul; Kadigrobov, Anatoli M.; Shekhter, Robert I.; Jonson, M.

    2013-12-01

    We show that nanomechanical shuttling of single electrons may enable qualitatively new functionality if spin-polarized electrons are injected into a nanoelectromechanical single-electron tunneling (NEM-SET) device. This is due to the combined effects of spin-dependent electron tunneling and Coulomb blockade of tunneling, which are phenomena that occur in certain magnetic NEM-SET devices. Two effects are predicted to occur in such structures. The first is a reentrant shuttle instability, by which we mean the sequential appearance, disappearance and again the appearance of a shuttle instability as the driving voltage is increased (or the mechanical dissipation is diminished). The second effect is an enhanced spin polarization of the nanomechanically assisted current flow.

  20. Evolution of the Shuttle extravehicular mobility unit

    NASA Technical Reports Server (NTRS)

    Correale, J. V.

    1979-01-01

    This paper deals briefly with the emergence of space suits as an important factor in supporting space missions but focuses mainly on the conceptual design of the Shuttle EMU (extravehicular mobility unit). Factors covering the criteria for the design approach are discussed. Several of the more specific subjects concerning the configuration selected are stressed; the EMU operating pressure, simplicity in don/doff, male/female sizing, components' useful life and the complexity surrounding the support of multi-year missions throughout the Shuttle program duration. In addition, comparisons with past EMU programs are made including a detailed discussion of how the Shuttle application and requirements drove and made this configuration a significant departure from previous EMU systems.

  1. Shuttle Liquid Fly Back Booster Configuration Options

    NASA Technical Reports Server (NTRS)

    Healy, Thomas J., Jr.

    1998-01-01

    This paper surveys the basic configuration options available to a Liquid Fly Back Booster (LFBB), integrated with the Space Shuttle system. The background of the development of the LFBB concept is given. The influence of the main booster engine (BME) installations and the fly back engine (FBE) installation on the aerodynamic configurations are also discussed. Limits on the LFBB configuration design space imposed by the existing Shuttle flight and ground elements are also described. The objective of the paper is to put the constrains and design space for an LFBB in perspective. The object of the work is to define LFBB configurations that significantly improve safety, operability, reliability and performance of the Shuttle system and dramatically lower operations costs.

  2. Shuttle inspection team recognized for 'eagle eyes.'

    NASA Technical Reports Server (NTRS)

    2000-01-01

    NASA Administrator Daniel Goldin (left) applauds the Space Shuttle ice and debris inspection team who were recognized for their keen safety observations prior to the launch of Space Shuttle Discovery. Standing next to Goldin are (left to right) D. Scott Otto, with Lockheed Martin Space Services Company; John B. Blue, Thomas F. Ford and Michael Barber, with United Space Alliance; Gregory N. Katnik and Jorge E. Rivera, with NASA. Katnick and Rivera received the agency's Exceptional Achievement Medal; Barber, Blue, Ford and Otto received the NASA Public Service Medal. While scanning the launch pad before launch, the team found a stray 4-inch pin near the Shuttle's external fuel tank that could have caused damage during launch. Discovery was safely launched the next day, on Oct. 11.

  3. Cryogenic recovery. [of space shuttle propellants

    NASA Technical Reports Server (NTRS)

    Howard, F. S.

    1976-01-01

    Because of the low boiling temperature of cryogenic propellants to be used on the Space Shuttle, loss of cryogens from boiloff could become very costly. This paper describes how this shuttle problem is being solved at Kennedy Space Center. Cryogenic losses are categorized relative to the particular cryogenic involved, the Space Shuttle servicing operation causing boiloff and the magnitude of the loss. The techniques under consideration are discussed in detail. These techniques include reclaiming the boiloff by reliquefaction, upgrading the reclaimed boiloff by purification, and interim boiloff storage in metal hydride prior to reprocessing. One of the reliquefaction processes discussed in detail utilizes the cooling effect of venting some of the liquid hydrogen boiloff to provide a simple hydrogen reliquefaction unit. Possible future applications of these cryogenics recovery techniques to industry and transportation systems using liquid hydrogen for energy storage and fuel are also discussed.

  4. Flight testing of the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Brand, V. D.

    1981-01-01

    An account is given of the flight testing development phase of the Space Shuttle Orbiter. Because of its low lift-to-drag ratio, the Orbiter must make its 280-kt landing approach in the lower atmosphere with a steep descent angle of 19 deg, pulling up at 1800 feet altitude to a 1-1.5 deg descent angle, and finally gliding to a landing at 195 kt. The Orbiter has almost neutral aerodynamic static stability in pitch and yaw, requiring the use of electronic stabilization to augment the vehicle's natural stability. Attention is given to the flight test program, which comprised the first four flights of the Space Shuttle, and which substantially relied on preflight analysis to establish confidence in Shuttle flight performance. Also discussed are the solar radiation absorption and aerodynamic heating characteristics

  5. STS-51 Space Shuttle Mission Report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1993-01-01

    The STS-51 Space Shuttle Program Mission Report summarizes the payloads as well as the orbiter, external tank (ET), solid rocket booster (SRB), redesigned solid rocket motor (RSRM), and the space shuttle main engine (SSME) systems performance during the fifty-seventh flight of the space shuttle program and seventeenth flight of the orbiter vehicle Discovery (OV-103). In addition to the orbiter, the flight vehicle consisted of an ET designated as ET-59; three SSME's, which were designated as serial numbers 2031, 2034, and 2029 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-060. The lightweight RSRM's that were installed in each SRB were designated as 360W033A for the left SRB and 360L033B for the right SRB.

  6. STS-40 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1991-01-01

    The STS-40 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem operations during the forty-first flight of the Space Shuttle and the eleventh flight of the Orbiter Vehicle Columbia (OV-102). In addition to the Columbia vehicle, the flight vehicle consisted of an External Tank (ET) designated as ET-41 (LWT-34), three Space Shuttle main engines (SSME's) (serial numbers 2015, 2022, and 2027 in positions 1, 2, and 3, respectively), and two Solid Rocket Boosters (SRB's) designated as BI-044. The primary objective of the STS-40 flight was to successfully perform the planned operations of the Spacelab Life Sciences-1 (SLS-1) payload. The secondary objectives of this flight were to perform the operations required by the Getaway Special (GAS) payloads and the Middeck O-Gravity Dynamics Experiment (MODE) payload.

  7. STS-56 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1993-01-01

    The STS-56 Space Shuttle Program Mission Report provides a summary of the Payloads, as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the fifty-fourth flight of the Space Shuttle Program and sixteenth flight of the Orbiter vehicle Discovery (OV-103). In addition to the Orbiter, the flight vehicle consisted of an ET (ET-54); three SSME's, which were designated as serial numbers 2024, 2033, and 2018 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-058. The lightweight RSRM's that were installed in each SRB were designated as 360L031A for the left SRB and 360L031B for the right SRB.

  8. Catalogs of Space Shuttle earth observations photography

    NASA Technical Reports Server (NTRS)

    Lulla, Kamlesh; Helfert, Michael

    1990-01-01

    A review is presented of postflight cataloging and indexing activities of mission data obtained from Space Shuttle earth observations photography. Each Space Shuttle mission acquires 1300-4400 photographs of the earth that are reviewed and interpreted by a team of photointerpreters and cataloging specialists. Every photograph's manual and electronic set of plots is compared for accuracy of its locational coordinates. This cataloging activity is a critical and principal part of postflight activity and ensures that the database is accurate, updated and consequently made meaningful for further utilization in the applications and research communities. A final product in the form of a Catalog of Space Shuttle Earth Observations Handheld Photography is published for users of this database.

  9. STS-36 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Mechelay, Joseph E.; Germany, D. M.; Nicholson, Leonard S.

    1990-01-01

    The STS-36 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities on this thirty-fourth flight of the Space Shuttle and the sixth flight of the OV-104 Orbiter vehicle, Atlantis. In addition to the Atlantis vehicle, the flight vehicle consisted of an External Tank (ET) (designated as ET-33/LWT-26), three Space Shuttle main engines (SSME's) (serial numbers 2019, 2030, and 2029), and two Solid Rocket Boosters (SRB's) (designated as BI-036). The STS-36 mission was a classified Department of Defense mission, and as such, the classified portions of the mission are not discussed. The unclassified sequence of events for this mission is shown in tabular form. Summarized are the significant problems that occurred in the Orbiter subsystems during the mission. The official problem tracking list is presented. In addition, each of the Orbiter problems is cited in the subsystem discussion.

  10. Post-Shuttle EVA Operations on ISS

    NASA Technical Reports Server (NTRS)

    West, Bill; Witt, Vincent; Chullen, Cinda

    2010-01-01

    The EVA hardware used to assemble and maintain the ISS was designed with the assumption that it would be returned to Earth on the Space Shuttle for ground processing, refurbishment, or failure investigation (if necessary). With the retirement of the Space Shuttle, a new concept of operations was developed to enable EVA hardware (EMU, Airlock Systems, EVA tools, and associated support equipment and consumables) to perform ISS EVAs until 2016 and possibly beyond to 2020. Shortly after the decision to retire the Space Shuttle was announced, NASA and the One EVA contractor team jointly initiated the EVA 2010 Project. Challenges were addressed to extend the operating life and certification of EVA hardware, secure the capability to launch EVA hardware safely on alternate launch vehicles, and protect EMU hardware operability on orbit for long durations.

  11. Shuttle Case Study Collection Website Development

    NASA Technical Reports Server (NTRS)

    Ransom, Khadijah S.; Johnson, Grace K.

    2012-01-01

    As a continuation from summer 2012, the Shuttle Case Study Collection has been developed using lessons learned documented by NASA engineers, analysts, and contractors. Decades of information related to processing and launching the Space Shuttle is gathered into a single database to provide educators with an alternative means to teach real-world engineering processes. The goal is to provide additional engineering materials that enhance critical thinking, decision making, and problem solving skills. During this second phase of the project, the Shuttle Case Study Collection website was developed. Extensive HTML coding to link downloadable documents, videos, and images was required, as was training to learn NASA's Content Management System (CMS) for website design. As the final stage of the collection development, the website is designed to allow for distribution of information to the public as well as for case study report submissions from other educators online.

  12. Orbital impacts and the Space Shuttle windshield

    NASA Technical Reports Server (NTRS)

    Edelstein, Karen S.

    1995-01-01

    The Space Transportation System (STS) fleet has flown more than sixty missions over the fourteen years since its first flight. As a result of encounters with on-orbit particulates (space debris and micrometeoroids), 177 impact features (chips) have been found on the STS outer windows (through STS-65). Forty-five of the damages were large enough to warrant replacement of the window. NASA's orbital operations and vehicle inspection procedures have changes over the history of the shuttle program, in response to concerns about the orbital environment and the cost of maintaining the space shuttle. These programmatic issues will be discussed, including safety concerns, maintenance issues, inspection procedures and flight rule changes. Examples of orbital debris impacts to the shuttle windows will be provided. There will also be a brief discussion of the impact properties of glass and what design changes have been considered to improve the impact properties of the windows.

  13. A perfect launch of Space Shuttle Discovery

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Space Shuttle Discovery lifts off Launch Pad 39A against a backdrop of xenon lights (just above the orbiter' nose and at left). On the Mobile Launcher Platform beneath, water begins flooding the area for flame and sound control. The perfect on- time liftoff occurred at 7:17 p.m. EDT, sending a crew of seven on the 100th launch in the history of the Shuttle program. Discovery carries a payload that includes the Integrated Truss Structure Z-1, first of 10 trusses that will form the backbone of the Space Station, and the third Pressurized Mating Adapter that will provide a Shuttle docking port for solar array installation on the sixth Station flight and Lab installation on the seventh Station flight. Discovery's landing is expected Oct. 22 at 2:10 p.m. EDT.

  14. Shuttle Orbiter Uplink Text and Graphics System

    NASA Technical Reports Server (NTRS)

    Hoover, A. A.; Land, C. K.; Lipoma, P. C.

    1978-01-01

    This paper presents the definition of requirements for and current design of the Shuttle Orbiter Uplink Text and Graphics System (UT&GS). Beginning in early 1981, the UT&GS will support Shuttle flights by providing the capability of transmitting single-frame imagery from the ground to the orbiting Shuttle vehicle. Such imagery is in the form of maps, text, diagrams, or photographs, and is outputted on the Orbiter as a paper hard copy. Four modes of operation will be provided to minimize the time required to transmit less than full-resolution imagery. This paper discusses the considerations and complications leading to the four modes and associated resolution requirements. The paper also presents the design of the CCD array ground scanner and airborne CRT hardcopier.

  15. Shuttle unified navigation filter, revision 1

    NASA Technical Reports Server (NTRS)

    Muller, E. S., Jr.

    1973-01-01

    Equations designed to meet the navigation requirements of the separate shuttle mission phases are presented in a series of reports entitled, Space Shuttle GN and C Equation Document. The development of these equations is based on performance studies carried out for each particular mission phase. Although navigation equations have been documented separately for each mission phase, a single unified navigation filter design is embodied in these separate designs. The purpose of this document is to present the shuttle navigation equations in a form in which they would most likely be coded-as the single unified navigation filter used in each mission phase. This document will then serve as a single general reference for the navigation equations replacing each of the individual mission phase navigation documents (which may still be used as a description of a particular navigation phase).

  16. Shuttle get-away special experiments

    NASA Technical Reports Server (NTRS)

    Orton, George

    1987-01-01

    This presentation describes two shuttle Get-Away-Special (GAS) experiments built by McDonnell Douglas to investigate low-g propellant acquisition and gaging. The first experiment was flown on shuttle mission 41-G in October 1984. The second experiment has been qualified for flight and is waiting for a flight assignment. The tests performed to qualify these experiments for flight are described, and the lessons learned which can be applied to future GAS experiments are discussed. Finally, survey results from 134 GAS experiments flown to date are presented. On the basis of these results it is recommended that future GAS experiments be qualified to shuttle thermal and dynamic environments through a rigorous series of mission operating tests. Furthermore, should automatic activation of the experiment be required during the boost phase of the mission, NASA-supplied redundant barometric switches should be employed to trigger the activation.

  17. Space Shuttle status and performance improvements

    NASA Technical Reports Server (NTRS)

    Day, L. E.

    1982-01-01

    Following nearly a decade of studies and development, the Space Shuttle is beginning its flight test phase. This paper reviews the status of the program, and preparations of the space vehicle and facilities for flight. The launch preparations, including conduct of the Mission Verification Test, which comprises a Flight Readiness Firing prior to the actual launch, is discussed. This simulation was designed to test the readiness of the complete organization and all ground and flight equipment for commitment to the first manned orbital flight. The plan of the orbital flight program is outlined indicating how each succeeding flight is made more complex and severe to incrementally test the Space Shuttle. Completion of this four flight program readies the Space Shuttle for operations in late 1982. Payload performance to orbit is increased as the program progresses through the development flights and on into operations.

  18. The defective nuclear lamina in Hutchinson-gilford progeria syndrome disrupts the nucleocytoplasmic Ran gradient and inhibits nuclear localization of Ubc9.

    PubMed

    Kelley, Joshua B; Datta, Sutirtha; Snow, Chelsi J; Chatterjee, Mandovi; Ni, Li; Spencer, Adam; Yang, Chun-Song; Cubeñas-Potts, Caelin; Matunis, Michael J; Paschal, Bryce M

    2011-08-01

    The mutant form of lamin A responsible for the premature aging disease Hutchinson-Gilford progeria syndrome (termed progerin) acts as a dominant negative protein that changes the structure of the nuclear lamina. How the perturbation of the nuclear lamina in progeria is transduced into cellular changes is undefined. Using patient fibroblasts and a variety of cell-based assays, we determined that progerin expression in Hutchinson-Gilford progeria syndrome inhibits the nucleocytoplasmic transport of several factors with key roles in nuclear function. We found that progerin reduces the nuclear/cytoplasmic concentration of the Ran GTPase and inhibits the nuclear localization of Ubc9, the sole E2 for SUMOylation, and of TPR, the nucleoporin that forms the basket on the nuclear side of the nuclear pore complex. Forcing the nuclear localization of Ubc9 in progerin-expressing cells rescues the Ran gradient and TPR import, indicating that these pathways are linked. Reducing nuclear SUMOylation decreases the nuclear mobility of the Ran nucleotide exchange factor RCC1 in vivo, and the addition of SUMO E1 and E2 promotes the dissociation of RCC1 and Ran from chromatin in vitro. Our data suggest that the cellular effects of progerin are transduced, at least in part, through reduced function of the Ran GTPase and SUMOylation pathways. PMID:21670151

  19. HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 Is Required for Circadian Periodicity through the Promotion of Nucleo-Cytoplasmic mRNA Export in Arabidopsis[W][OPEN

    PubMed Central

    MacGregor, Dana R.; Gould, Peter; Foreman, Julia; Griffiths, Jayne; Bird, Susannah; Page, Rhiannon; Stewart, Kelly; Steel, Gavin; Young, Jack; Paszkiewicz, Konrad; Millar, Andrew J.; Halliday, Karen J.; Hall, Anthony J.; Penfield, Steven

    2013-01-01

    Cold acclimation has been shown to be attenuated by the degradation of the INDUCER OF CBF EXPRESSION1 protein by the E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 (HOS1). However, recent work has suggested that HOS1 may have a wider range of roles in plants than previously appreciated. Here, we show that hos1 mutants are affected in circadian clock function, exhibiting a long-period phenotype in a wide range of temperature and light environments. We demonstrate that hos1 mutants accumulate polyadenylated mRNA in the nucleus and that the circadian defect in hos1 is shared by multiple mutants with aberrant mRNA export, but not in a mutant attenuated in nucleo-cytoplasmic transport of microRNAs. As revealed by RNA sequencing, hos1 exhibits gross changes to the transcriptome with genes in multiple functional categories being affected. In addition, we show that hos1 and other previously described mutants with altered mRNA export affect cold signaling in a similar manner. Our data support a model in which altered mRNA export is important for the manifestation of hos1 circadian clock defects and suggest that HOS1 may indirectly affect cold signaling through disruption of the circadian clock. PMID:24254125

  20. Phospho-regulation and nucleocytoplasmic trafficking of CrzA in response to calcium and alkaline-pH stress in Aspergillus nidulans.

    PubMed

    Hernández-Ortiz, Patricia; Espeso, Eduardo A

    2013-08-01

    Tolerance to abiotic stresses by microorganisms require of appropriate signalling and regulatory pathways. Calcineurin phosphatases mediate calcium-dependent signalling pathways which are widely distributed among phylogeny. In Saccharomyces cerevisiae, calcineurin mediates the post-translational modification of downstream effectors, most of them transcription factors, being the best-characterized calcineurin-regulated zinc-finger factor 1, Crz1p. Here we study the signalling process of CrzA, a filamentous fungal Crz orthologue, in response to calcium and ambient-pH alkalinization. In Aspergillus nidulans resting cells CrzA locates in the cytoplasm being excluded from nuclei. CrzA is a phospho-protein and upon calcium, manganese or alkaline-pH stresses, accumulates in nuclei in a calcineurin-dependent manner. Functional analysis of CrzA defined the presence of a nuclear-export and two nuclear-localization signals as well as a PSINVE sequence that constitutes the major calcineurin-docking domain. First 450 amino acids of CrzA contain these functional motifs and in this region is where phosphorylated residues locate. Different phosphorylation steps are identified in CrzA and activities of casein kinase 1 homologue, CkiA, and of glycogen synthase kinase-3β, identified for the first time here as GskA, are involved. The phospho-signalling process and nucleocytoplasmic trafficking of CrzA shows similarities to those described in yeast for Crz1p homologues and of NFATs in mammals. PMID:23772954

  1. STS-62 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1994-01-01

    The STS-62 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSHE) systems performance during the sixty-first flight of the Space Shuttle Program and sixteenth flight of the Orbiter vehicle Columbia (OV-102). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-62; three SSME's which were designated as serial numbers 2031, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-064. The RSRM's that were installed in each SRB were designated as 360L036A (lightweight) for the left SRB, and 36OWO36B (welterweight) for the right SRB. This STS-62 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objectives of the STS-62 mission were to perform the operations of the United States Microgravity Payload-2 (USMP-2) and the Office of Aeronautics and Space Technology-2 (OAST-2) payload. The secondary objectives of this flight were to perform the operations of the Dexterous End Effector (DEE), the Shuttle Solar Backscatter Ultraviolet/A (SSBUV/A), the Limited Duration Space Environment Candidate Material Exposure (LDCE), the Advanced Protein Crystal Growth (APCG), the Physiological Systems Experiments (PSE), the Commercial Protein Crystal Growth (CPCG), the Commercial Generic Bioprocessing Apparatus (CGBA), the Middeck Zero-Gravity Dynamics Experiment (MODE), the Bioreactor Demonstration System (BDS), the Air Force Maui Optical Site Calibration Test (AMOS), and the Auroral Photography Experiment (APE-B).

  2. An Analysis of Shuttle Crew Scheduling Violations

    NASA Technical Reports Server (NTRS)

    Bristol, Douglas

    2012-01-01

    From the early years of the Space Shuttle program, National Aeronautics and Space Administration (NASA) Shuttle crews have had a timeline of activities to guide them through their time on-orbit. Planners used scheduling constraints to build timelines that ensured the health and safety of the crews. If a constraint could not be met it resulted in a violation. Other agencies of the federal government also have scheduling constraints to ensure the safety of personnel and the public. This project examined the history of Space Shuttle scheduling constraints, constraints from Federal agencies and branches of the military and how these constraints may be used as a guide for future NASA and private spacecraft. This was conducted by reviewing rules and violations with regard to human aerospace scheduling constraints, environmental, political, social and technological factors, operating environment and relevant human factors. This study includes a statistical analysis of Shuttle Extra Vehicular Activity (EVA) related violations to determine if these were a significant producer of constraint violations. It was hypothesized that the number of SCSC violations caused by EVA activities were a significant contributor to the total number of violations for Shuttle/ISS missions. Data was taken from NASA data archives at the Johnson Space Center from Space Shuttle/ISS missions prior to the STS-107 accident. The results of the analysis rejected the null hypothesis and found that EVA violations were a significant contributor to the total number of violations. This analysis could help NASA and commercial space companies understand the main source of constraint violations and allow them to create constraint rules that ensure the safe operation of future human private and exploration missions. Additional studies could be performed to evaluate other variables that could have influenced the scheduling violations that were analyzed.

  3. STS-62 Space Shuttle mission report

    NASA Astrophysics Data System (ADS)

    Fricke, Robert W., Jr.

    1994-05-01

    The STS-62 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSHE) systems performance during the sixty-first flight of the Space Shuttle Program and sixteenth flight of the Orbiter vehicle Columbia (OV-102). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-62; three SSME's which were designated as serial numbers 2031, 2109, and 2029 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-064. The RSRM's that were installed in each SRB were designated as 360L036A (lightweight) for the left SRB, and 36OWO36B (welterweight) for the right SRB. This STS-62 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objectives of the STS-62 mission were to perform the operations of the United States Microgravity Payload-2 (USMP-2) and the Office of Aeronautics and Space Technology-2 (OAST-2) payload. The secondary objectives of this flight were to perform the operations of the Dexterous End Effector (DEE), the Shuttle Solar Backscatter Ultraviolet/A (SSBUV/A), the Limited Duration Space Environment Candidate Material Exposure (LDCE), the Advanced Protein Crystal Growth (APCG), the Physiological Systems Experiments (PSE), the Commercial Protein Crystal Growth (CPCG), the Commercial Generic Bioprocessing Apparatus (CGBA), the Middeck Zero-Gravity Dynamics Experiment (MODE), the Bioreactor Demonstration System (BDS), the Air Force Maui Optical Site Calibration Test (AMOS), and the Auroral Photography Experiment (APE-B).

  4. STS-59 Space Shuttle mission report

    NASA Astrophysics Data System (ADS)

    Fricke, Robert W., Jr.

    1994-06-01

    The STS-59 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the sixty-second flight of the Space Shuttle Program and sixth flight of the Orbiter vehicle Endeavor (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-63; three SSME's which were designated as serial numbers 2028, 2033, and 2018 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-065. The RSRM's that were installed in each SRB were designated as 360W037A (welterweight) for the left SRB, and 360H037B (heavyweight) for the right SRB. This STS-59 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objective of the STS-59 mission was to successfully perform the operations of the Space Radar Laboratory-1 (SRL-1). The secondary objectives of this flight were to perform the operations of the Space Tissue Loss-A (STL-A) and STL-B payloads, the Visual Function Tester-4 (VFT-4) payload, the Shuttle Amateur Radio Experiment-2 (SAREX-2) experiment, the Consortium for Materials Development in Space Complex Autonomous Payload-4 (CONCAP-4), and the three Get-Away Special (GAS) payloads.

  5. Scintillation Effects on Space Shuttle GPS Data

    NASA Technical Reports Server (NTRS)

    Goodman, John L.; Kramer, Leonard

    2001-01-01

    Irregularities in ionospheric electron density result in variation in amplitude and phase of Global Positioning System (GPS) signals, or scintillation. GPS receivers tracking scintillated signals may lose carrier phase or frequency lock in the case of phase sc intillation. Amplitude scintillation can cause "enhancement" or "fading" of GPS signals and result in loss of lock. Scintillation can occur over the equatorial and polar regions and is a function of location, time of day, season, and solar and geomagnetic activity. Mid latitude regions are affected only very rarely, resulting from highly disturbed auroral events. In the spring of 1998, due to increasing concern about scintillation of GPS signals during the upcoming solar maximum, the Space Shuttle Program began to assess the impact of scintillation on Collins Miniaturized Airborne GPS Receiver (MAGR) units that are to replace Tactical Air Control and Navigation (TACAN) units on the Space Shuttle orbiters. The Shuttle Program must determine if scintillation effects pose a threat to safety of flight and mission success or require procedural and flight rule changes. Flight controllers in Mission Control must understand scintillation effects on GPS to properly diagnose "off nominal" GPS receiver performance. GPS data from recent Space Shuttle missions indicate that the signals tracked by the Shuttle MAGR manifest scintillation. Scintillation is observed as anomalous noise in velocity measurements lasting for up to 20 minutes on Shuttle orbit passes and are not accounted for in the error budget of the MAGR accuracy parameters. These events are typically coincident with latitude and local time occurrence of previously identified equatorial spread F within about 20 degrees of the magnetic equator. The geographic and seasonal history of these events from ground-based observations and a simple theoretical model, which have potential for predicting events for operational purposes, are reviewed.

  6. STS-60 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1994-01-01

    The STS-60 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the sixtieth flight of the Space Shuttle Program and eighteenth flight of the Orbiter vehicle Discovery (OV-103). In addition to the Orbiter, the flight vehicle consisted of an ET designated at ET-61 (Block 10); three SSME's which were designated as serial numbers 2012, 2034, and 2032 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-062. The RSRM's that were installed in each SRB were designated as 360L035A (lightweight) for the left SRB, and 360Q035B (quarterweight) for the right SRB. This STS-60 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume VIII, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objectives of the STS-60 mission were to deploy and retrieve the Wake Shield Facility-1 (WSF-1), and to activate the Spacehab-2 payload and perform on-orbit experiments. Secondary objectives of this flight were to activate and command the Capillary Pumped Loop/Orbital Debris Radar Calibration Spheres/Breman Satellite Experiment/Getaway Special (GAS) Bridge Assembly (CAPL/ODERACS/BREMSAT/GBA) payload, the Auroral Photography Experiment-B (APE-B), and the Shuttle Amateur Radio Experiment-II (SAREX-II).

  7. STS-59 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1994-01-01

    The STS-59 Space Shuttle Program Mission Report summarizes the Payload activities as well as the Orbiter, External Tank (ET), Solid Rocket Booster (SRB), Redesigned Solid Rocket Motor (RSRM), and the Space Shuttle main engine (SSME) systems performance during the sixty-second flight of the Space Shuttle Program and sixth flight of the Orbiter vehicle Endeavor (OV-105). In addition to the Orbiter, the flight vehicle consisted of an ET designated as ET-63; three SSME's which were designated as serial numbers 2028, 2033, and 2018 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-065. The RSRM's that were installed in each SRB were designated as 360W037A (welterweight) for the left SRB, and 360H037B (heavyweight) for the right SRB. This STS-59 Space Shuttle Program Mission Report fulfills the Space Shuttle Program requirement as documented in NSTS 07700, Volume 8, Appendix E. That document requires that each major organizational element supporting the Program report the results of its hardware evaluation and mission performance plus identify all related in-flight anomalies. The primary objective of the STS-59 mission was to successfully perform the operations of the Space Radar Laboratory-1 (SRL-1). The secondary objectives of this flight were to perform the operations of the Space Tissue Loss-A (STL-A) and STL-B payloads, the Visual Function Tester-4 (VFT-4) payload, the Shuttle Amateur Radio Experiment-2 (SAREX-2) experiment, the Consortium for Materials Development in Space Complex Autonomous Payload-4 (CONCAP-4), and the three Get-Away Special (GAS) payloads.

  8. The Toxoplasma gondii kinetochore is required for centrosome association with the centrocone (spindle pole)

    PubMed Central

    Farrell, Megan; Gubbels, Marc-Jan

    2013-01-01

    Summary The kinetochore is a multi-protein structure assembled on eukaryotic centromeres mediating chromosome attachment to spindle microtubules. Here we identified the kinetochore proteins Nuf2 and Ndc80 in the apicomplexan parasite Toxoplasma gondii. Localization revealed that kinetochores remain clustered throughout the cell cycle and co-localize with clustered centromeres at the centrocone, a structure containing the spindle pole embedded in the nuclear envelope. Pharmacological disruption of microtubules resulted in partial loss of some kinetochore and centromere clustering, indicating microtubules are necessary but not strictly required for kinetochore clustering. Generation of a TgNuf2 conditional knockdown strain revealed it is essential for chromosome segregation, but dispensable for centromere clustering. The centromeres actually remained associated with the centrocone suggesting microtubule binding is not required for their interaction with the spindle pole. The most striking observation upon TgNuf2 depletion was that the centrosome behaved normally, but that it lost its association with the centrocone. This suggests that microtubules are essential to maintain contact between the centrosome and chromosomes, and this interaction is critical for the partitioning of the nuclei into the two daughter parasites. Finally, genetic complementation experiments with mutated TgNuf2 constructs highlighted an apicomplexan specific motif with a putative role in nuclear localization. PMID:24015880

  9. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis

    PubMed Central

    Pillai, Smitha; Nguyen, Jonathan; Johnson, Joseph; Haura, Eric; Coppola, Domenico; Chellappan, Srikumar

    2015-01-01

    TANK Binding Kinase 1 (TBK1) is a non-canonical IκB kinase that contributes to KRAS-driven lung cancer. Here we report that TBK1 plays essential roles in mammalian cell division. Specifically, levels of active phospho-TBK1 increase during mitosis and localize to centrosomes, mitotic spindles and midbody, and selective inhibition or silencing of TBK1 triggers defects in spindle assembly and prevents mitotic progression. TBK1 binds to the centrosomal protein CEP170 and to the mitotic apparatus protein NuMA, and both CEP170 and NuMA are TBK1 substrates. Further, TBK1 is necessary for CEP170 centrosomal localization and binding to the microtubule depolymerase Kif2b, and for NuMA binding to dynein. Finally, selective disruption of the TBK1–CEP170 complex augments microtubule stability and triggers defects in mitosis, suggesting that TBK1 functions as a mitotic kinase necessary for microtubule dynamics and mitosis. PMID:26656453

  10. Analyses of Shuttle Orbiter approach and landing

    NASA Technical Reports Server (NTRS)

    Ashkenas, I. L.; Hoh, R. H.; Teper, G. L.

    1982-01-01

    A study of the Shuttle Orbiter approach and landing conditions is summarized. The causes of observed PIO-like flight deficiencies are listed, and possible corrective measures are examined. Closed-loop pilot/vehicle analyses are described, and a description is given of path-attitude stability boundaries. The latter novel approach is found to be of great value in delineating and illustrating the basic causes of this multiloop pilot control problem. It is shown that the analytical results are consistent with flight test and fixed-base simulation. Conclusions are drawn concerning possible improvements in the Shuttle Orbiter/Digital Flight Control System.

  11. An optical communication link on the Shuttle

    NASA Astrophysics Data System (ADS)

    Grady, Jane L.

    A communication link for the transfer of data between the crew cabin and payload bay of the Shuttle is proposed. The configuration of the system, which is to transmit data optically through the Shuttle cabin window, is described. The effects of temperature cycling and cosmic radiation on optical fibers, the cable, connectors, and lenses, and of vibrations during a mission on the alignment of collimating lenses at the window are investigated. Consideration is given to determining the optimum design for the optical fiber and the optimum system operation wavelength for transmission through the aft Orbiter window and the payload environment.

  12. Viscoelastic propellant effects on Space Shuttle Dynamics

    NASA Technical Reports Server (NTRS)

    Bugg, F.

    1981-01-01

    The program of solid propellant research performed in support of the space shuttle dynamics modeling effort is described. Stiffness, damping, and compressibility of the propellant and the effects of many variables on these properties are discussed. The relationship between the propellant and solid rocket booster dynamics during liftoff and boost flight conditions and the effects of booster vibration and propellant stiffness on free free solid rocket booster modes are described. Coupled modes of the shuttle system and the effect of propellant stiffness on the interfaces of the booster and the external tank are described. A finite shell model of the solid rocket booster was developed.

  13. Holography on the NASA Space Shuttle

    NASA Technical Reports Server (NTRS)

    Wuerker, R. F.; Heflinger, L. O.; Flannery, J. V.; Kassel, A.; Rollauer, A. M.

    1980-01-01

    The SL-3 flight on the Space Shuttle will carry a 25 mW He-Ne laser holographic recorder for recording the solution growth of triglycine sulfate (TGS) crystals under low-zero gravity conditions. Three hundred holograms (two orthogonal views) will be taken (on SO-253 film) of each growth experiment. Processing and analysis (i.e., reconstructed imagery, holographic schlieren, reverse reference beam microscopy, and stored beam interferometry) of the holographic records will be done at NASA/MSFC. Other uses of the recorder on the Shuttle have been proposed.

  14. A Representative Shuttle Environmental Control System

    NASA Technical Reports Server (NTRS)

    Brose, H. F.; Stanley, M. D.; Leblanc, J. C.

    1977-01-01

    The Representative Shuttle Environmental Control System (RSECS) provides a ground test bed to be used in the early accumulation of component and system operating data, the evaluation of potential system improvements, and possibly the analysis of Shuttle Orbiter test and flight anomalies. Selected components are being subjected to long term tests to determine endurance and corrosion resistance capability prior to Orbiter vehicle experience. Component and system level tests in several cases are being used to support flight certification of Orbiter hardware. These activities are conducted as a development program to allow for timeliness, flexibility, and cost effectiveness not possible in a program burdened by flight documentation and monitoring constraints.

  15. Study of alternate space shuttle concepts

    NASA Technical Reports Server (NTRS)

    1971-01-01

    A study of alternate space shuttle concepts was conducted to examine the stage-and-one-half concept and its potential for later conversion and use in the two stage reusable shuttle system. A study of external hydrogen tank concepts was conducted to determine the issues involved in the design and production of a low-cost expendable tank system. The major objectives of the study were to determine: (1) realistic drop tank program cost estimates, (2) estimated drop tank program cost for selected specific designs, and (3) change in program cost due to variations in design and manufacturing concepts and changes in program assumptions.

  16. First Space Shuttle External Tank Rollout

    NASA Technical Reports Server (NTRS)

    1977-01-01

    The first Space Shuttle External Tank, the Main Propulsion Test Article (MPTA), rolls off the assembly line September 9, 1977 at the Michoud Assembly Facility in New Orleans. The MPTA was then transported to the National Space Technology Laboratories in southern Mississippi where it was used in the first static firing of the three main engines. Marshall Space Flight Center had management responsibility for Space Shuttle propulsion elements, including the External Tank. Martin Marietta was the prime contractor who designed and assembled the tanks at Michoud.

  17. Space Shuttle Orbital Drag Parachute Design

    NASA Technical Reports Server (NTRS)

    Meyerson, Robert E.

    2001-01-01

    The drag parachute system was added to the Space Shuttle Orbiter's landing deceleration subsystem beginning with flight STS-49 in May 1992. The addition of this subsystem to an existing space vehicle required a detailed set of ground tests and analyses. The aerodynamic design and performance testing of the system consisted of wind tunnel tests, numerical simulations, pilot-in-the-loop simulations, and full-scale testing. This analysis and design resulted in a fully qualified system that is deployed on every flight of the Space Shuttle.

  18. Post-Shuttle EVA Operations on ISS

    NASA Technical Reports Server (NTRS)

    West, William; Witt, Vincent; Chullen, Cinda

    2010-01-01

    The expected retirement of the NASA Space Transportation System (also known as the Space Shuttle ) by 2011 will pose a significant challenge to Extra-Vehicular Activities (EVA) on-board the International Space Station (ISS). The EVA hardware currently used to assemble and maintain the ISS was designed assuming that it would be returned to Earth on the Space Shuttle for refurbishment, or if necessary for failure investigation. With the retirement of the Space Shuttle, a new concept of operations was developed to enable EVA hardware (Extra-vehicular Mobility Unit (EMU), Airlock Systems, EVA tools, and associated support hardware and consumables) to perform ISS EVAs until 2015, and possibly beyond to 2020. Shortly after the decision to retire the Space Shuttle was announced, the EVA 2010 Project was jointly initiated by NASA and the One EVA contractor team. The challenges addressed were to extend the operating life and certification of EVA hardware, to secure the capability to launch EVA hardware safely on alternate launch vehicles, to protect for EMU hardware operability on-orbit, and to determine the source of high water purity to support recharge of PLSSs (no longer available via Shuttle). EVA 2010 Project includes the following tasks: the development of a launch fixture that would allow the EMU Portable Life Support System (PLSS) to be launched on-board alternate vehicles; extension of the EMU hardware maintenance interval from 3 years (current certification) to a minimum of 6 years (to extend to 2015); testing of recycled ISS Water Processor Assembly (WPA) water for use in the EMU cooling system in lieu of water resupplied by International Partner (IP) vehicles; development of techniques to remove & replace critical components in the PLSS on-orbit (not routine); extension of on-orbit certification of EVA tools; and development of an EVA hardware logistical plan to support the ISS without the Space Shuttle. Assumptions for the EVA 2010 Project included no more

  19. Shuttle Student Involvement Project for Secondary Schools

    NASA Technical Reports Server (NTRS)

    Wilson, G. P.; Ladwig, A.

    1981-01-01

    The National Aeronautics and Space Administration (NASA) has initiated the Shuttle Student Involvement Project for Secondary Schools (SSIP-S), an annual nationwide competition to select student proposals for experiments suitable for flight aboard the Space Shuttle. The objective of the project is to stimulate the study of science and technology in grades 9 through 12 by directly relating students to a space research program. This paper will analyze the first year of the project from a standpoint of how the competition was administered; the number and types of proposals that were submitted; and will discuss the process involved in preparing the winning experiments for eventual flight.

  20. Scientific uses of the space shuttle

    NASA Technical Reports Server (NTRS)

    1974-01-01

    A survey was conducted to determine the possible missions which could be accomplished by the space shuttle. The areas of scientific endeavor which were considered are as follows: (1) atmospheric and space physics, (2) high energy astrophysics, (3) infrared astronomy, (4) optical and ultraviolet astronomy, (5) solar physics, (6) life sciences, and (7) planetary exploration. Specific projects to be conducted in these broader areas are defined. The modes of operation of the space shuttle are analyzed. Instruments and equipment required for conducting the experiments are identified.

  1. A next generation Space Shuttle concept

    NASA Technical Reports Server (NTRS)

    Davis, Hubert P.; Teixeira, Charles

    1989-01-01

    Work done over the past two years for the NASA/Johnson Space Center on possible evolutionary paths of the present Space Shuttle Orbiter and system to meet the goals of enhanced safety, mission suitability economy, and robustness is summarized in this paper. Three vehicle concepts are described: an advanced technology one-and-one-half stage vehicle, a near-term evolved Space Shuttle system closely related to today's Orbiter in concept and appearance, and an Orbiter-Glider which might be appropriate for use with emerging large space launch vehicles.

  2. Human inositol 1,4,5-trisphosphate 3-kinase isoform B (IP3KB) is a nucleocytoplasmic shuttling protein specifically enriched at cortical actin filaments and at invaginations of the nuclear envelope.

    PubMed

    Nalaskowski, Marcus M; Fliegert, Ralf; Ernst, Olga; Brehm, Maria A; Fanick, Werner; Windhorst, Sabine; Lin, Hongying; Giehler, Susanne; Hein, Jamin; Lin, Yuan-Na; Mayr, Georg W

    2011-02-11

    Recent studies have shown that inositol 1,4,5-trisphosphate 3-kinase isoform B (IP3KB) possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells. Detailed mechanisms of its targeting regulation will be important in understanding the role of Ins(1,4,5)P(3) phosphorylation on subcellular calcium signaling and compartment-specific initiation of pathways leading to regulatory active higher phosphorylated inositol phosphates. Here, we identified an exportin 1-dependent nuclear export signal ((134)LQRELQNVQV) and characterized the amino acids responsible for nuclear localization of IP3KB ((129)RKLR). These two targeting domains regulate the amount of nuclear IP3KB in cells. We also demonstrated that the localization of IP3KB at the plasma membrane is due to its binding to cortical actin structures. Intriguingly, all three of these targeting activities reside in one small polypeptide segment (amino acids 104-165), which acts as a multitargeting domain (MTD). Finally, a hitherto unknown subnuclear localization of IP3KB could be demonstrated in rapidly growing H1299 cells. IP3KB is specifically enriched at nuclear invaginations extending perpendicular between the apical and basal surface of the nucleus of these flat cells. Such nuclear invaginations are known to be involved in Ins(1,4,5)P(3)-mediated Ca(2+) signaling of the nucleus. Our findings indicate that IP3KB not only regulates cytoplasmic Ca(2+) signals by phosphorylation of subplasmalemmal and cytoplasmic Ins(1,4,5)P(3) but may also be involved in modulating nuclear Ca(2+) signals generated from these nuclear envelope invaginations. PMID:21148483

  3. Aerodynamic and base heating studies on space shuttle configurations

    NASA Technical Reports Server (NTRS)

    1974-01-01

    Heating rate and pressure measurements were obtained on a 25-O space shuttle model in a vacuum chamber. Correlation data on windward laminar and turbulent boundary layers and leeside surfaces of the space shuttle orbiter are included.

  4. Behind the Scenes: Leinbach Is Shuttle's 'Button Pusher'

    NASA Video Gallery

    He's the guy who, figuratively, pushes "the big red button" to send the shuttle into space. Astronaut Mike Massimino takes you inside the firing room at the Kennedy Space Center to meet Shuttle Lau...

  5. Space Shuttle Endeavour Heads West - Duration: 111 seconds.

    NASA Video Gallery

    NASA's Shuttle Carrier Aircraft, a modified 747, flew retired shuttle Endeavour from Kennedy Space Center in Florida to Houston on Sept. 19, 2012, to complete the first leg of Endeavour's trip to L...

  6. Toward a history of the space shuttle. An annotated bibliography

    NASA Astrophysics Data System (ADS)

    Launius, Roger D.; Gillette, Aaron K.

    1992-12-01

    This selective, annotated bibliography discusses those works judged to be most essential for researchers writing scholarly studies on the Space Shuttle's history. A thematic arrangement of material concerning the Space Shuttle will hopefully bring clarity and simplicity to such a complex subject. Subjects include the precursors of the Space Shuttle, its design and development, testing and evaluation, and operations. Other topics revolve around the Challenger accident and its aftermath, promotion of the Space Shuttle, science on the Space Shuttle, commercial uses, the Space Shuttle's military implications, its astronaut crew, the Space Shuttle and international relations, the management of the Space Shuttle Program, and juvenile literature. Along with a summary of the contents of each item, judgments have been made on the quality, originality, or importance of some of these publications. An index concludes this work.

  7. Launch of space shuttle Challenger on the 41-C mission

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Wide angle view of the launch of the space shuttle Challenger on the 41-C mission from the Kennedy Space Center (KSC) launch pad. This view was taken from the Shuttle training aircraft by Astronaut John Young.

  8. Shuttle Atlantis: From the Inside - Duration: 8 minutes, 39 seconds.

    NASA Video Gallery

    An unprecedented up close, inside look at space shuttle Atlantis as it was readied for "towback"" from Kennedy's Shuttle Landing Facility runway to Orbiter Processing Facility-1 following its May 2...

  9. STS-1: the first space shuttle mission, April 12, 1981

    NASA Video Gallery

    Space shuttle Columbia launched on the first space shuttle mission on April 12, 1981, a two-day demonstration of the first reusable, piloted spacecraft's ability to go into orbit and return safely ...

  10. Toward a history of the space shuttle. An annotated bibliography

    NASA Technical Reports Server (NTRS)

    Launius, Roger D. (Compiler); Gillette, Aaron K. (Compiler)

    1992-01-01

    This selective, annotated bibliography discusses those works judged to be most essential for researchers writing scholarly studies on the Space Shuttle's history. A thematic arrangement of material concerning the Space Shuttle will hopefully bring clarity and simplicity to such a complex subject. Subjects include the precursors of the Space Shuttle, its design and development, testing and evaluation, and operations. Other topics revolve around the Challenger accident and its aftermath, promotion of the Space Shuttle, science on the Space Shuttle, commercial uses, the Space Shuttle's military implications, its astronaut crew, the Space Shuttle and international relations, the management of the Space Shuttle Program, and juvenile literature. Along with a summary of the contents of each item, judgments have been made on the quality, originality, or importance of some of these publications. An index concludes this work.

  11. Space Operations Center, shuttle interaction study, volume 1

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The implication of using the Shuttle with the SOC, including constraints that the Shuttle places upon the SOC design is studied. The considerations involved in the use of the Shuttle as a part of the SOC concept, and the constraints to the SOC imposed by the Shuttle in its interactions with the SOC, and on the design or technical solutions which allow satisfactory accomplishment of the interactions are identified.

  12. Economic analysis of the space shuttle system, volume 1

    NASA Technical Reports Server (NTRS)

    1972-01-01

    An economic analysis of the space shuttle system is presented. The analysis is based on economic benefits, recurring costs, non-recurring costs, and ecomomic tradeoff functions. The most economic space shuttle configuration is determined on the basis of: (1) objectives of reusable space transportation system, (2) various space transportation systems considered and (3) alternative space shuttle systems.

  13. STS-37 Space Shuttle mission report

    NASA Astrophysics Data System (ADS)

    Fricke, Robert W.

    1991-05-01

    The STS-37 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities during this thirty-ninth flight of the Space Shuttle and the eighth flight of the Orbiter Vehicle Atlantis (OV-104). In addition to the Atlantis vehicle, the flight vehicle consisted of the following: an External Tank (ET) (designated as ET-37/LWT-30); three Space Shuttle main engines (SSME's) (serial numbers 2019, 2031, and 2107 in positions 1, 2, and 3, respectively); and two Solid Rocket Boosters (SRB's) designated as BI-042. The primary objective of this flight was to successfully deploy the Gamma Ray Observatory (GRO) payload. The secondary objectives were to successfully perform all operations necessary to support the requirements of the Protein Crystal Growth (PCG) Block 2 version, Radiation Monitoring Experiment-3 (RME-3), Ascent Particle Monitor (APM), Shuttle Amateur Radio Experiment-2 (SAREX-2), Air Force Maui Optical Site Calibration Test (AMOS), Bioserve Instrumentation Technology Associates Materials Dispersion Apparatus (BIMDA), and the Crew and Equipment Transfer Aids (CETA) payloads.

  14. Shuttle applications in tropospheric air quality observations

    NASA Technical Reports Server (NTRS)

    Friedman, E.; Gupta, J.; Carmichael, J.

    1978-01-01

    The role which might be played by the space shuttle in obtaining data which describes the air quality of the north-eastern United States was investigated. The data requirements of users, a model for statistical interpretation of the observations, the influence of orbit parameters on the spatial and temporal sampling and an example of application of the the model were considered.

  15. Computer Program for Space-Shuttle Testing

    NASA Technical Reports Server (NTRS)

    Hyman, M. D.; Fine, G. H.; Hollombe, G. J.

    1986-01-01

    Demand on Space Shuttle general-purpose computers reduced. Simulations Testbed and Scenario Pre-processor (STB&SPP) system reduces need for use of GPC's in hardware and software development and testing. System consists of computer program, SPP, and set of utility subroutines, STB, which incorporates Interface Simulator (ISIM). STB&SPP system written in FORTRAN V and Assembler.

  16. Program for Space Shuttle Payload Cabling

    NASA Technical Reports Server (NTRS)

    Schultz, Roger D.; Saxon, C. Rogers

    1987-01-01

    EXCABL is expert-system computer program developed to route electrical cables in Space Shuttle Orbiter payload bay for each mission. Automates cable-routing process and provides data for cable-installation documents. Automation increased speed and accuracy of payload-integration process, and expert system codifies knowledge cabling experts have acquired. Written in ART.

  17. Identification of space shuttle main engine dynamics

    NASA Technical Reports Server (NTRS)

    Duyar, Ahmet; Guo, Ten-Huei; Merrill, Walter C.

    1989-01-01

    System identification techniques are used to represent the dynamic behavior of the Space Shuttle Main Engine. The transfer function matrices of the linearized models of both the closed loop and the open loop system are obtained by using the recursive maximum likelihood method.

  18. Laser contouring of Space Shuttle tiles

    NASA Astrophysics Data System (ADS)

    Bishop, P. J.; Minardi, A.; He, Mingli; Shelton, Bret

    Straight through and partial cuts were made in fibrous silicon-based ceramic insulation materials (used on the Space Shuttle) to determine the feasibility of laser machining. Experimental results were accumulated from over 800 exposures to determine the belt conditions for cutting. Laser intensity, feedrate, and other parameters were varied to determine conditions for cutting and are discussed in the paper.

  19. Launch of STS-66 Space Shuttle Atlantis

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The Space Shuttle Atlantis returns to work after a refurbishing and a two-year layoff, as liftoff for NASA's STS-66 occurs at noon (EDT), November 3, 1994. A 70mm camera was used to record the image. Note the vegetation and the reflection of the launch in the water across from the launch pad.

  20. Launch of STS-66 Space Shuttle Atlantis

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The Space Shuttle Atlantis returns to work after a refurbishing and a two-year layoff, as liftoff for NASA's STS-66 occurs at noon (EDT), November 3, 1994. A 35mm camera was used to record the image, which includes much of the base of the launch site as well as the launch itself.

  1. Liftoff of STS-59 Shuttle Endeavour

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The liftoff of the Space Shuttle Endeavour is backdropped against a dawn sky at the Kennedy Space Center (KSC). The morning sky allows for a contrasting backdrop for the diamond shock effect of the thrust from Endeavour's main engines. Trees outline the lower portion of the view. Liftoff occurred at 7:05 a.m., April 9, 1994.

  2. Liftoff of STS-59 Shuttle Endeavour

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The liftoff of the Space Shuttle Endeavour is backdropped against a dawn sky at the Kennedy Space Center (KSC). Trees and water from a nearby marsh outline the lower portion of the view. Liftoff occurred at 7:05 a.m., April 9, 1994.

  3. STS-67 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1995-01-01

    The STS-67 Space Shuttle Program Mission Report provides the results of the orbiter vehicle performance evaluation during this sixty-eighth flight of the Shuttle Program, the forty-third flight since the return to flight, and the eighth flight of the Orbiter vehicle Endeavour (OV-105). In addition, the report summarizes the payload activities and the performance of the External Tank (ET), Solid Rocket Booster (SRB), Reusable Solid Rocket Motor (RSRM), and the Space Shuttle Main Engines (SSME). The serial numbers of the other elements of the flight vehicle were ET-69 for the ET; 2012, 2033, and 2031 for SSME's 1, 2, and 3, respectively; and Bl-071 for the SRB's. The left-hand RSRM was designated 360W043A, and the right-hand RSRM was designated 360L043B. The primary objective of this flight was to successfully perform the operations of the ultraviolet astronomy (ASTRO-2) payload. Secondary objectives of this flight were to complete the operations of the Protein Crystal Growth - Thermal Enclosure System (PCG-TES), the Protein Crystal Growth - Single Locker Thermal Enclosure System (PCG-STES), the Commercial Materials Dispersion Apparatus ITA Experiments (CMIX), the Shuttle Amateur Radio Experiment-2 (SAREX-2), the Middeck Active Control Experiment (MACE), and two Get-Away Special (GAS) payloads.

  4. Space shuttle program: Lightning protection criteria document

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The lightning environment for space shuttle design is defined and requirements that the design must satisfy to insure protection of the vehicle system from direct and indirect effects of lightning are imposed. Specifications, criteria, and guidelines included provide a practical and logical approach to protection problems.

  5. STS-44 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1992-01-01

    The STS-44 Space Shuttle Program Mission Report is a summary of the vehicle subsystem operations during the forty-fourth flight of the Space Shuttle Program and the tenth flight of the Orbiter vehicle Atlantis (OV-104). In addition to the Atlantis vehicle, the flight vehicle consisted of the following: an External Tank (ET) designated as ET-53 (LWT-46); three Space Shuttle main engines (SSME's) (serial numbers 2015, 2030, and 2029 in positions 1, 2, and 3, respectively); and two Solid Rocket Boosters (SRB's) designated as BI-047. The lightweight redesigned Solid Rocket Motors (RSRM's) installed in each one of the SRB's were designated as 360L019A for the left SRB and 360W019B for the right SRB. The primary objective of the STS-44 mission was to successfully deploy the Department of Defense (DOD) Defense Support Program (DSP) satellite/inertial upper stage (IUS) into a 195 nmi. earth orbit at an inclination of 28.45 deg. Secondary objectives of this flight were to perform all operations necessary to support the requirements of the following: Terra Scout, Military Man in Space (M88-1), Air Force Maui Optical System Calibration Test (AMOS), Cosmic Radiation Effects and Activation Monitor (CREAM), Shuttle Activation Monitor (SAM), Radiation Monitoring Equipment-3 (RME-3), Visual Function Tester-1 (VFT-1), and the Interim Operational Contamination Monitor (IOCM) secondary payloads/experiments.

  6. Shuttle-Car System for Continuous Mining

    NASA Technical Reports Server (NTRS)

    Collins, E. R., Jr.

    1984-01-01

    Buffer storage catches coal production between loadings. Telescoping reservoir filled continuously. With tailgate down, shuttle car slides into place along sides and bottom of reservoir. Reservoir retracts along inside of car and out through tailgate, leaving coal behind in car. System not restricted to coal mining and may prove economical for hauling other solid materials.

  7. Space shuttle visual simulation system design study

    NASA Technical Reports Server (NTRS)

    1973-01-01

    A recommendation and a specification for the visual simulation system design for the space shuttle mission simulator are presented. A recommended visual system is described which most nearly meets the visual design requirements. The cost analysis of the recommended system covering design, development, manufacturing, and installation is reported. Four alternate systems are analyzed.

  8. STS-37 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1991-01-01

    The STS-37 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities during this thirty-ninth flight of the Space Shuttle and the eighth flight of the Orbiter Vehicle Atlantis (OV-104). In addition to the Atlantis vehicle, the flight vehicle consisted of the following: an External Tank (ET) (designated as ET-37/LWT-30); three Space Shuttle main engines (SSME's) (serial numbers 2019, 2031, and 2107 in positions 1, 2, and 3, respectively); and two Solid Rocket Boosters (SRB's) designated as BI-042. The primary objective of this flight was to successfully deploy the Gamma Ray Observatory (GRO) payload. The secondary objectives were to successfully perform all operations necessary to support the requirements of the Protein Crystal Growth (PCG) Block 2 version, Radiation Monitoring Experiment-3 (RME-3), Ascent Particle Monitor (APM), Shuttle Amateur Radio Experiment-2 (SAREX-2), Air Force Maui Optical Site Calibration Test (AMOS), Bioserve Instrumentation Technology Associates Materials Dispersion Apparatus (BIMDA), and the Crew and Equipment Transfer Aids (CETA) payloads.

  9. Space Shuttle MMOD Threat Mitigation Techniques

    NASA Technical Reports Server (NTRS)

    Hyde, Justin L.; Christiansen, Eric L.; Kerr, James H.

    2007-01-01

    Prior to each shuttle mission, threat assessments are performed to determine the risk of critical penetration, payload bay door radiator tube leak and crew module window replacement from Micrometeoroid and Orbital Debris (MMOD). Mission parameters, such as vehicle attitude, exposure time and altitude are used as inputs for the analysis. Ballistic limit equations, based on hypervelocity impact testing of shuttle materials are used to estimate the critical particle diameters of the outer surfaces of the vehicle. The assessments are performed using the BUMPER computer code at the NASA/JSC Hypervelocity Impact Technology Facility (HITF). The most critical involves the calculation of Loss of Crew and Vehicle (LOCV) risk. In recent years, NASA has implemented several techniques to reduce the risk to the Shuttle from MMOD impacts. This paper will describe on-orbit inspection of the reinforced carbon-carbon (RCC) regions and the methods used discern hypervelocity impact damage. Impact damage contingency plans and on-orbit repair techniques will also be discussed. The wing leading edge impact detection system (WLEIDS) and it's role in the reduction of on-orbit risk reduction will be presented. Finally, an analysis of the effectivity of alternative shuttle flight attitudes on MMOD risk will be demonstrated.

  10. STS-41 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Camp, David W.; Germany, D. M.; Nicholson, Leonard S.

    1990-01-01

    The STS-41 Space Shuttle Program Mission Report contains a summary of the vehicle subsystem activities on this thirty-sixth flight of the Space Shuttle and the eleventh flight of the Orbiter vehicle, Discovery (OV-103). In addition to the Discovery vehicle, the flight vehicle consisted of an External Tank (ET) (designated as ET-39/LWT-32), three Space Shuttle main engines (SSME's) (serial numbers 2011, 2031, and 2107), and two Solid Rocket Boosters (SRB's), designated as BI-040. The primary objective of the STS-41 mission was to successfully deploy the Ulysses/inertial upper stage (IUS)/payload assist module (PAM-S) spacecraft. The secondary objectives were to perform all operations necessary to support the requirements of the Shuttle Backscatter Ultraviolet (SSBUV) Spectrometer, Solid Surface Combustion Experiment (SSCE), Space Life Sciences Training Program Chromosome and Plant Cell Division in Space (CHROMEX), Voice Command System (VCS), Physiological Systems Experiment (PSE), Radiation Monitoring Experiment - 3 (RME-3), Investigations into Polymer Membrane Processing (IPMP), Air Force Maui Optical Calibration Test (AMOS), and Intelsat Solar Array Coupon (ISAC) payloads. The sequence of events for this mission is shown in tabular form. Summarized are the significant problems that occurred in the Orbiter subsystems during the mission. The official problem tracking list is presented. In addition, each Orbiter problem is cited in the subsystem discussion.

  11. Using Space Shuttle Data in the Classroom.

    ERIC Educational Resources Information Center

    Feldman, Allan P.

    1984-01-01

    Describes how to use data from television broadcasts of shuttle launches along with data from National Aeronautics and Space Administration (NASA) "Educational Briefs for the Classroom" to establish relevant connections of high school physics with the real world. Includes graphs of range/altitude, speed/time, and three tables with launch data and…

  12. Space shuttle visual simulation system design study

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The current and near-future state-of-the-art in visual simulation equipment technology is related to the requirements of the space shuttle visual system. Image source, image sensing, and displays are analyzed on a subsystem basis, and the principal conclusions are used in the formulation of a recommended baseline visual system. Perceptibility and visibility are also analyzed.

  13. Space Shuttle. Teacher's Guide [and] Student Material.

    ERIC Educational Resources Information Center

    Butler, Della

    The teacher's guide and student materials provide elementary and junior high school students with an understanding of the space shuttle as a new kind of transportation for conveying goods and performing services in space. The unit is appropriate for a learning center approach, individual instruction, or use with the entire class. It is organized…

  14. Rockwell Fails in Response to Shuttle Disaster.

    ERIC Educational Resources Information Center

    Kaufman, John A.

    1988-01-01

    Describes the contingent media relations policy employed by Rockwell International, the prime contractor for the United States space shuttle program, following the January 28, 1986, destruction of the Challenger. Analyzes Rockwell's response through a theoretical model of crisis perception and Rockwell's policy in relation to the mass media. (MS)

  15. Cryogenic spin testing of NASA's shuttle engines

    NASA Astrophysics Data System (ADS)

    Maillar, Kenneth M.; Enos, Anthony; Gauthier, Robert

    1992-12-01

    Spin testing of the Space Shuttle Main Engine (SSME) high-pressure turbopump rotors is described focusing on the SSME cryogenic spin test facility. Testing at full operating speed is predicated on achieving and maintaining a cryogenic rotor temperature. Rotors are driven to operational speeds after being chilled to - 195 C.

  16. STS-50 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W.

    1992-01-01

    The STS-50 Space Shuttle Program Mission Report contains a summary of the Orbiter, External Tank (ET), Solid Rocket Booster/Redesigned Solid Rocket Motor (SRB/RSRM), and the Space Shuttle main engine (SSME) subsystem performance during the forty-eighth flight of the Space Shuttle Program, and the twelfth flight of the Orbiter vehicle Columbia (OV-102). In addition to the Columbia vehicle, the flight vehicle consisted of the following: an ET which was designated ET-50 (LUT-43); three SSME's which were serial numbers 2019, 2031, and 2011 in positions 1, 2, and 3, respectively; and two SRB's which were designated BI-051. The lightweight/redesigned RSRM's installed in each SRB were designated 360L024A for the left RSRM and 360M024B for the right RSRM. The primary objective of the STS-50 flight was to successfully perform the planned operations of the United States Microgravity Laboratory (USML-1) payload. The secondary objectives of this flight were to perform the operations required by the Investigations into Polymer Membrane Processing (IPMP), and the Shuttle Amateur Radio Experiment 2 (SAREX-2) payloads. An additional secondary objective was to meet the requirements of the Ultraviolet Plume Instrument (UVPI), which was flown as a payload of opportunity.

  17. STS-52 Space Shuttle mission report

    NASA Technical Reports Server (NTRS)

    Fricke, Robert W., Jr.

    1992-01-01

    The STS-52 Space Shuttle Program Mission Report provides a summary of the Orbiter, External Tank (ET), Solid Rocket Booster/Redesigned Solid Rocket Motor (SRB/RSRM), and the Space Shuttle main engine (SSME) subsystem performance during the fifty-first flight of the Space Shuttle Program, and the thirteenth flight of the Orbiter vehicle Columbia (OV-102). In addition to the Orbiter, the flight vehicle consisted of the following: an ET (designated as ET-55/LWT-48); three SSME's, which were serial numbers 2030, 2015, and 2034 in positions 1, 2, and 3, respectively; and two SRB's, which were designated BI-054. The lightweight RSRM's that were installed in each SRB were designated 360L027A for the left SRB and 360Q027B for the right SRB. The primary objectives of this flight were to successfully deploy the Laser Geodynamic Satellite (LAGEOS-2) and to perform operations of the United States Microgravity Payload-1 (USMP-1). The secondary objectives of this flight were to perform the operations of the Attitude Sensor Package (ASP), the Canadian Experiments-2 (CANEX-2), the Crystals by Vapor Transport Experiment (CVTE), the Heat Pipe Performance Experiment (HPP), the Commercial Materials Dispersion Apparatus Instrumentation Technology Associates Experiments (CMIX), the Physiological System Experiment (PSE), the Commercial Protein Crystal Growth (CPCG-Block 2), the Shuttle Plume Impingement Experiment (SPIE), and the Tank Pressure Control Experiment (TPCE) payloads.

  18. Reliability of a Shuttle reaction timer

    NASA Technical Reports Server (NTRS)

    Hays, Russell D.; Mazzocca, Augustus D.; Rashid, Michael; Siconolfi, Steven F.

    1992-01-01

    Reaction, movement, and task times refer to the times needed to initially respond to a stimulus, make the specific movement, and complete the entire task. This study evaluated the reliability of a simple reaction timer designed to mimic a Space Shuttle task (turning on an overhead switch).

  19. Space Shuttle Atlantis after RSS rollback

    NASA Technical Reports Server (NTRS)

    2001-01-01

    This closeup reveals Space Shuttle Atlantis after rollback of the Rotating Service Structure. Extended to the side of Atlantis is the orbiter access arm, with the White Room at its end. The White Room provides entry for the crew into Atlantis's cockpit. Below Atlantis, on either side of the tail are the tail service masts. They support the fluid, gas and electrical requirements of the orbiter's liquid oxygen and liquid hydrogen aft T-0 umbilicals. Atlantis is carrying the U.S. Laboratory Destiny, a key module in the growth of the International Space Station. Destiny will be attached to the Unity node on the Space Station using the Shuttle's robotic arm. Three spacewalks are required to complete the planned construction work during the 11-day mission. Launch is targeted for 6:11 p.m. EST and the planned landing at KSC Feb. 18 about 1:39 p.m. This mission marks the seventh Shuttle flight to the Space Station, the 23rd flight of Atlantis and the 102nd flight overall in NASA's Space Shuttle program.

  20. Microbiology studies in the Space Shuttle

    NASA Technical Reports Server (NTRS)

    Taylor, G. R.

    1976-01-01

    Past space microbiology studies have evaluated three general areas: microbe detection in extraterrestrial materials; monitoring of autoflora and medically important species on crewmembers, equipment, and cabin air; and in vitro evaluations of isolated terrestrial species carried on manned and unmanned spaceflights. These areas are briefly reviewed to establish a basis for presenting probable experiment subjects applicable to the Space Shuttle era. Most extraterrestrial life detection studies involve visitations to other heavenly bodies. Although this is not applicable to the first series of Shuttle flights, attempts to capture meteors and spores in space could be important. Human pathogen and autoflora monitoring will become more important with increased variety among crewmembers. Inclusion of contaminated animal and plant specimens in the space lab will necessitate inflight evaluation of cross-contamination and infection potentials. The majority of Shuttle microbiology studies will doubtless fall into the third study area. Presence of a space lab will permit a whole range of experimentation under conditions similar to these experienced in earth-based laboratories. The recommendations of various study groups are analyzed, and probable inflight microbiological experiment areas are identified for the Life Sciences Shuttle Laboratory.