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1

Characterization of the antioxidant properties of phenolic extracts from some citrus peels  

Microsoft Academic Search

This study sought to determine the distribution of free and bound phenolics in some Nigerian citrus peels [orange (Citrus sinensis), grapefruit (Citrus paradisii) and shaddock (Citrus maxima)] and characterize the antioxidant properties. The free phenolics were extracted with 80% acetone, while the bound phenolics\\u000a were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Free phenolic extracts had

G. Oboh; A. O. Ademosun

2

Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme *  

PubMed Central

In order to investigate the antioxidant properties of the polysaccharides from the brown alga Sargassum fusiforme, the crude polysaccharides from S. fusiforme (SFPS) were extracted in hot water, and the lipid peroxidation inhibition assay exhibited that SFPS possessed a potential antioxidant activity. Hence, two purely polymeric fractions, SFPS-1 and SFPS-2 were isolated by the column of DEAE (2-diethylaminoethanol)-Sepharose Fast Flow, with their molecular weights of 51.4 and 30.3 kDa determined by high performance gel permeation chromatography (HPGPC). They were preliminarily characterized using chemical analysis in combination of infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies and found to contain large amounts of uronic acids and ?-glycosidical linkages. The antioxidant activities of these two SFPS fractions were evaluated using superoxide and hydroxyl radical-scavenging assays. The results show that the antioxidant ability of SFPS-2 was higher than that of SFPS-1, probably correlating with the molecular weight and uronic acid content.

Zhou, Jing; Hu, Nan; Wu, Ya-lin; Pan, Yuan-jiang; Sun, Cui-rong

2008-01-01

3

Saponins in Ipomoea batatas tubers: Isolation, characterization, quantification and antioxidant properties  

Microsoft Academic Search

Triterpene saponins are a class of plant natural products with a wide range of bioactivities, which makes them an interesting research subject. This work reports, for the first time, the isolation and characterization of saponins in Ipomoeabatatas tuber flour, their quantification and antioxidant properties. Their structures were characterized on the basis of UV, FAB–MS, ESI–MS, GC–MS, polarimetry and NMR data,

Irene Dini; Gian Carlo Tenore; Antonio Dini

2009-01-01

4

Preparation, characterization, and antioxidant properties of gallic acid- grafted-chitosans  

Microsoft Academic Search

Gallic acid-grafted-chitosans (GA-g-chitosans) with four different grafting ratios were prepared by a free radical-induced grafting reaction in order to improve antioxidant and water-solubility. To verify the synthesis of GA-g-chitosans, 1H NMR and thin layer chromatography were employed, and the results revealed that GA was grafted onto the chitosan. The antioxidant properties of the GA-g-chitosans were evaluated using several in vitro

Young-Sook Cho; Se-Kwon Kim; Chang-Bum Ahn; Jae-Young Je

2011-01-01

5

Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional chinese medicines.  

PubMed

There is considerable interest in the isolation of more potent antioxidant compounds to treat diseases involving oxidative stress. Thirty-three traditional Chinese medicine (TCM) extracts were examined for their antioxidant activity using the 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonate] (ABTS) assay. Five extracts with high activity (Cratoxylum cochinchinense, Cortex magnoliae officinalis, Psoralea corylifolia L, Curculigo orchioides Gaertn, and Glycyrrhiza uralensis Fisch.) were selected for further characterization. C. cochinchinense outperformed other extracts in most of the assays tested except phospholipid peroxidation inhibition, where P. corylifolia L showed higher activity. C. cochinchinense was particularly potent in inhibiting the formation of advanced glycation end products on proteins and strongly inhibited hypochlorous acid-induced DNA damage. We attempted to isolate the active ingredients from C. cochinchinense and obtained an extract (YCT) containing at least 90% mangiferin as identified by HPLC and mass spectrometry. However, YCT showed significantly higher activity in assays of phospholipid peroxidation, inhibition of protein glycation, and superoxide (O(2)(?-)) and peroxynitrite (ONOO(-)) scavenging, as compared with mangiferin, suggesting that the nonmangiferin constituents of YCT contribute to its additional antioxidant activities. PMID:15182859

Tang, Soon Yew; Whiteman, Matthew; Peng, Zhao Feng; Jenner, Andrew; Yong, Eu Leong; Halliwell, Barry

2004-06-15

6

Preparation, characterization and antioxidant property of water-soluble ferulic acid grafted chitosan.  

PubMed

The objective of the present work was to improve the antioxidant activity and water solubility of chitosan by grafting with ferulic acid through a carbodiimide-mediated coupling reaction. UV-vis spectrophotometry, FTIR, (1)H NMR and ninhydrin assay confirmed the grafting of ferulic acid onto chitosan at the C-2 position. Ferulic acid grafted chitosan - prepared using a mole ratio of chitosan to ferulic acid of 1:1, reaction temperature of 60°C, and reaction time of 3h - possessed the highest ferulic acid substitution degree, i.e. 0.37. Although ferulic acid grafted chitosan showed reduced crystallinity (?10%) and decreased decomposition temperature (?55°C) as compared to chitosan, it exhibited greater radical scavenging activity (?55%) and was soluble in water (up to 1.3mg/mL). The improved antioxidant property and water solubility of this chitosan derivative could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical, pharmaceutical and cosmetics industries. PMID:23768592

Woranuch, Sarekha; Yoksan, Rangrong

2013-04-12

7

[Antioxidant properties of plant extracts].  

PubMed

Investigation of antioxidant properties of some plants was carried out. A group of plants exhibited antimicrobial activity was studied in detail. Efficiency of plants as antioxidants was tested by the influence of their extracts on the yield of photochemiluminescence of Gly-Trp solutions. Antioxidant properties were examined under conditions when their own absorption was minimized. Riboflavin as additional sensitizer was used in this experiment for superoxide generation. The antioxidant effect was evaluated with regard to single dose of plant extracts and their concentration in human organism. The effect decreases in the following consequence: Hypericum perforatum > Potentilla erectra > Ledum palustre > Plantago major > Salvia officinalis > Chamomilla recutita > Arctostaphylos uva. PMID:9591094

Bol'shakova, I V; Lozovskaia, E L; Sapezhinski?, I I

8

Antioxidant properties of aminoguanidine.  

PubMed

It is well known that aminoguanidine (AG) can diminish advanced glycosylation of proteins, which might be beneficial in preventing chronic diabetic complications. Recent reports suggested an inter-relationship between glycosylation of protein and free radical damage. In the present study, we examined the free radical scavenging properties of AG. Electron paramagnetic resonance using the spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was performed to determine the superoxide and hydroxyl radical scavenging abilities of AG. These experiments revealed that AG was an effective hydroxyl radical scavenger even though it expressed a direct inhibitory effect on the xanthine oxidase activity at high concentrations (AG > or = 5 mM). In the second part of the study, allophycocyanin was used as an indicator of free radical mediated protein damage. In the assay, 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH) was used as a peroxyl radical generator, and the loss of allophycocyanin fluorescence was monitored. The antioxidant effect of AG was expressed in oxygen-radical absorbing capacity (ORAC), where one ORAC unit equals the net protection produced by 1 microM Trolox (a water soluble analogue of vitamin E) as a control standard. AG exhibited a significant dose-dependent effect against free radical damage. These radical scavenging properties of AG may contribute to protective effects during glycation and explain the prevention of diabetic complications. PMID:10520725

Courderot-Masuyer, C; Dalloz, F; Maupoil, V; Rochette, L

1999-01-01

9

Isolation and characterization of dihydromonacolin-MV from Monascus purpureus for antioxidant properties.  

PubMed

The methanolic extract of Monascus purpureus cultivated by solid-state fermentation on rice showed strong 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and better yield as compared to other polarity based extracted fractions. It was selected for further purification of the antioxidant. The activity-guided repeated fractionation of methanolic extract on a silica gel column chromatography yielded a compound that exhibited strong antioxidant activity. Based on the spectroscopic analysis by UV, IR, 1H NMR, 13C NMR, 2D-HSQCT NMR, and MS, the antioxidant isolated was elucidated as a derivative of dihydromonacolin-K, where the ester group is 2-methyl propionate, designated as dihydromonacolin-MV. The DPPH radical was significantly scavenged by the dihydromonacolin-MV (IC50 20+/-1 microg ml-1). The dihydromonacolin-MV showed strong inhibition of lipid peroxidation in a liposome model with an IC50 value of 5.71+/-0.38 microg ml-1 and superoxide radical scavenging activity with an IC50 value of 163.97+/-2.68 microg ml-1. PMID:17043831

Dhale, Mohan A; Divakar, S; Kumar, S Umesh; Vijayalakshmi, G

2006-10-17

10

Antioxidant properties of thiamine.  

PubMed

Thiamine (10(-4)-10(-6)M) inhibits lipid peroxidation in rat liver microsomes and free radical oxidation of oleic acid in vitro. Thiamine interacts with free radicals and hydroperoxides and is oxidized to thiochrome and thiamine disulfide. The antioxidant effect of thiamine is probably related to successive transfer of 2H(+)from the NH(2)group of the pyrimidine ring and H(+)from the thiazole ring (after its opening) to reactive substrates. PMID:11177269

Lukienko, P I; Mel'nichenko, N G; Zverinskii, I V; Zabrodskaya, S V

2000-09-01

11

Electrochemical characterization of the marine antioxidant gadusol.  

PubMed

The electrochemical properties of gadusol, a metabolite extracted from fish roes, were determined for the first time by cyclic- and square-wave voltammetries on a glassy carbon electrode in buffered aqueous solutions. The aims of the work were characterization of the redox behavior and assessment of the mechanism underlying the antioxidant capacity of the compound. Anodic waves with peak potentials of 710 +/- 5 mV and 601 +/- 9 mV vs. Ag/AgCl were obtained for gadusol and its anion gadusolate, respectively. The voltammograms point to the irreversible nature of the oxidation process and reveal better antioxidant properties for the compound under physiological pH, with a moderate reductive power, but improved stability under atmospheric oxidation in comparison with other natural antioxidants such as ascorbic acid. The presence of direct ionic micelles prepared with sodium dodecyl-sulfate or cetyl-trimethyl-ammonium chloride yielded no significant differences regarding homogeneous medium in the cyclic voltammetry of gadusolate. The results provide evidence of the electron transfer mechanism for the quenching reaction of standard-reactive species by gadusolate and support it as a promising candidate to be tested as an antioxidant additive to food, cosmetic and pharmaceutical formulas. The direct analysis of the metabolite in biological systems and natural extracts by electrochemical methods is also envisaged. PMID:23074911

Arbeloa, Ernesto Maximiliano; Luján Ramírez, Cristina; Ariel Procaccini, Raúl; Churio, María Sandra

2012-09-01

12

Synthesis and antioxidant properties of fullero-steroidal covalent conjugates  

Microsoft Academic Search

Four new fullero-steroids were synthesized starting from ?-aminobutyric acid (GABA)-incorporated fulleropyrrolidine and corresponding sterols. The synthesis, characterization, preliminary study on in vitro antioxidant activity as well as the steady state photophysical properties are presented. All synthesized compounds showed antioxidant activity 2–3-fold higher than the parent fullerene. The most active compound appeared to be as potent as standard antioxidant BHT (2,6-di-tert-butyl-4-methylphenol).

Mira S. Bjelakovi?; Dejan M. Godjevac; Dragana R. Mili?

2007-01-01

13

Antioxidant properties of essential oils  

Microsoft Academic Search

By the method of capillary gas-liquid chromatography, we studied the antioxidant properties and stability during the storage\\u000a of hexane solutions of 14 individual essential oils from black and white pepper (Piper nigrum L.), cardamom (Elettaria cardamomum L.), nutmeg (Myristica fragrans Houtt.), mace (Myristica fragrans Houtt), juniper berry (Juniperus communis L.), fennel seed (Foeniculum vulgare Mill., var. dulce Thelling), caraway (Carvum

T. A. Misharina; M. B. Terenina; N. I. Krikunova

2009-01-01

14

[Antioxidant properties of essential oils].  

PubMed

By the method of capillary gas-liquid chromatography we studied antioxidant properties and stability during the storage of hexane solutions of 14 individual essential oils from black and white pepper (Piper nigrum L.), cardamom (Elettaria cardamomum L.), nutmeg (Myristica fragrans Houtt.), mace (Myristica fragrans Houtt), juniperberry (Juniperus communis L.), seed of fennel (Foeniculum vulgare Mill., var. dulce Thelling), caraway (Carvum carvi L.), dry leaves of cinnamon (Cinnamomum zeylanicum Bl.), marjoram (Origanum majorana L.), laurel (Laurus nobilis L.), ginger (Zingiber officinale L.), garlic (Allium sativum L.), and clove bud (Caryophyllus aromaticus L.). We assessed the antioxidant properties by the oxidation of aliphatic aldehyde (trans-2-hexenal) into the according carbon acid. We established that essential oils of garlic, clove bud, ginger and leaves of cinnamon have the maximal efficiency of inhibition of hexenal oxidation (80-93%), while black pepper oil has the minimal (49%). Antioxidant properties of essential oils with a high content of substituted phenols depended poorly on its concentration in model systems. We studied the changes in essential oils content during the storage of its hexane solutions for 40 days in the light and out of the light and compared it with the stability of essential oils stored for a year out of the light. PMID:20067158

Misharina, T A; Terenina, M B; Krikunova, N I

15

Antitrypanosomal and antioxidant properties of 4-hydroxycoumarins derivatives.  

PubMed

In the present communication we prepared a series of six 4-hydroxycoumarin derivatives, isosters of quercetin, recognized as an antioxidant natural compound, with the aim of evaluating the antitrypanosomal activity against Trypanosoma cruzi, the parasite responsible for Chagas disease, and the antioxidant properties. We have used the 4-hydroxycoumarin moiety (compound 1) as the molecular template for the synthesis of compounds 2-7. These derivates have shown moderate trypanocidal activity. However they have been proved to be good antioxidants. In particular compound 7 is the most active antioxidant and it is, therefore, a potential candidate for a successful employment in conditions characterized by free radicals overproduction. PMID:22832320

Pérez-Cruz, Fernanda; Serra, Silvia; Delogu, Giovanna; Lapier, Michel; Maya, Juan Diego; Olea-Azar, Claudio; Santana, Lourdes; Uriarte, Eugenio

2012-07-13

16

Physicochemical and antioxidant properties of Algerian honey.  

PubMed

The aim of the present study was to characterize the physical, biochemical and antioxidant properties of Algerian honey samples (n = 4). Physical parameters, such as pH, moisture content, electrical conductivity (EC), total dissolved solids (TDS), color intensity, total sugar and sucrose content were measured. Several biochemical and antioxidant tests were performed to determine the antioxidant properties of the honey samples. The mean pH was 3.84 ± 0.01, and moisture the content was 13.21 ± 0.16%. The mean EC was 0.636 ± 0.001, and the mean TDS was 316.92 ± 0.92. The mean color was 120.58 ± 0.64 mm Pfund, and the mean 5-hydroxymethylfurfural (HMF) content was 21.49 mg/kg. The mean total sugar and reducing sugar contents were 67.03 ± 0.68 g/mL and 64.72 ± 0.52 g/g, respectively. The mean sucrose content was 2.29 ± 0.65%. High mean values of phenolic (459.83 ± 1.92 mg gallic acid/kg), flavonoid (54.23 ± 0.62 mg catechin/kg), ascorbic acid (159.70 ± 0.78 mg/kg), AEAC (278.15 ± 4.34 mg/kg), protein (3381.83 ± 6.19 mg/kg) and proline (2131.47 ± 0.90) contents, as well as DPPH (39.57% ± 4.18) and FRAP activities [337.77 ± 1.01 µM Fe (II)/100 g], were also detected, indicating that Algerian honey has a high antioxidant potential. Strong positive correlations were found between flavonoid, proline and ascorbic acid contents and color intensity with DPPH and FRAP values. Thus, the present study revealed that Algerian honey is a good source of antioxidants. PMID:22996344

Khalil, Ibrahim; Moniruzzaman, Mohammed; Boukraâ, Laïd; Benhanifia, Mokhtar; Islam, Asiful; Islam, Nazmul; Sulaiman, Siti Amrah; Gan, Siew Hua

2012-09-20

17

Antioxidant properties of humic substances.  

PubMed

Humic substances (HS) are heterogeneous, redox-active organic macromolecules. While electron transfer to and from HS under reducing conditions is well investigated, comparatively little is known on the electron donating (i.e., antioxidant) properties of HS under oxic conditions. In this work, the electron donating capacities (EDCs) of terrestrial and aquatic HS were quantified by mediated electrochemical oxidation over a wide range of pH values and applied redox potentials (E(h)) using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as an electron transfer mediator. Electrochemical oxidation of three model humic acids (HAs) was largely irreversible, and the EDCs of these HAs increased with increasing E(h) and pH. These results suggest that HS contain a wide variety of moieties that are oxidized at different potentials and that, upon oxidation, release protons and undergo irreversible follow-up reactions. At a given pH and E(h), the EDCs of the HS correlated well with their titrated phenol contents suggesting phenolic moieties as major electron donating groups in HS. Comparing the EDCs of 15 HS with their electron accepting capacities (EACs), aquatic HS had higher EDCs and lower EACs than terrestrial HS of comparable aromaticities. These results indicate that oxidative transformation of HS in the environment results in a depletion of electron donating phenolic moieties with antioxidant properties relative to the electron accepting quinone moieties. PMID:22463073

Aeschbacher, Michael; Graf, Cornelia; Schwarzenbach, René P; Sander, Michael

2012-04-13

18

Phlorotannin extracts from fucales characterized by HPLC-DAD-ESI-MSn: approaches to hyaluronidase inhibitory capacity and antioxidant properties.  

PubMed

Purified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSn. Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activity and the hyaluronidase (HAase) inhibitory capacity exhibited by the extracts were also assessed. A correlation between the extracts activity and their chemical composition was established. F. spiralis, the species presenting higher molecular weight phlorotannins, generally displayed the strongest lipid peroxidation inhibitory activity (IC?? = 2.32 mg/mL dry weight) and the strongest HAase inhibitory capacity (IC?? = 0.73 mg/mL dry weight). As for superoxide radical scavenging, C. nodicaulis was the most efficient species (IC?? = 0.93 mg/mL dry weight), followed by F. spiralis (IC?? = 1.30 mg/mL dry weight). These results show that purified phlorotannin extracts have potent capabilities for preventing and slowing down the skin aging process, which is mainly associated with free radical damage and with the reduction of hyaluronic acid concentration, characteristic of the process. PMID:23222802

Ferreres, Federico; Lopes, Graciliana; Gil-Izquierdo, Angel; Andrade, Paula B; Sousa, Carla; Mouga, Teresa; Valentão, Patrícia

2012-12-10

19

Phlorotannin Extracts from Fucales Characterized by HPLC-DAD-ESI-MSn: Approaches to Hyaluronidase Inhibitory Capacity and Antioxidant Properties  

PubMed Central

Purified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSn. Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activity and the hyaluronidase (HAase) inhibitory capacity exhibited by the extracts were also assessed. A correlation between the extracts activity and their chemical composition was established. F. spiralis, the species presenting higher molecular weight phlorotannins, generally displayed the strongest lipid peroxidation inhibitory activity (IC50 = 2.32 mg/mL dry weight) and the strongest HAase inhibitory capacity (IC50 = 0.73 mg/mL dry weight). As for superoxide radical scavenging, C. nodicaulis was the most efficient species (IC50 = 0.93 mg/mL dry weight), followed by F. spiralis (IC50 = 1.30 mg/mL dry weight). These results show that purified phlorotannin extracts have potent capabilities for preventing and slowing down the skin aging process, which is mainly associated with free radical damage and with the reduction of hyaluronic acid concentration, characteristic of the process.

Ferreres, Federico; Lopes, Graciliana; Gil-Izquierdo, Angel; Andrade, Paula B.; Sousa, Carla; Mouga, Teresa; Valentao, Patricia

2012-01-01

20

Antioxidant Properties of Ethanolic Extract from Ramulus mori (Sangzhi)  

Microsoft Academic Search

The antioxidant properties and total phenolic contents of four fractions of ethanolic extract from Ramulus mori were examined. Various experimental models including superoxide radical, hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) scavenging activity, metal chelating activity, and reducing power were used for characterization of their antioxidant activity. The four fractions showed various degrees of efficacy in each assay in a dose-dependent manner.

Zuofa Zhang; Jie Jin; Liangen Shi

2009-01-01

21

The antioxidant properties of serum albumin  

Microsoft Academic Search

Free radicals are a normal component of cellular oxygen metabolism in mammals. However, free radical-associated damage is an important factor in many pathological processes. Glycation and oxidative damage cause protein modifications, frequently observed in numerous diseases. Albumin represents a very abundant and important circulating antioxidant. This review brings together recent insights on albumin antioxidant properties. First, it focuses on the

Marjolaine Roche; Philippe Rondeau; Nihar Ranjan Singh; Evelyne Tarnus; Emmanuel Bourdon

2008-01-01

22

Antioxidant properties of various solvent extracts of mulberry ( Morus indica L.) leaves  

Microsoft Academic Search

The antioxidant properties and total phenolic contents of methanol, acetone and water extracts of mulberry (Morus indica L.) leaves were examined. Various experimental models including iron (III) reducing capacity, total antioxidant capacity, DPPH radical scavenging activity and in vitro inhibition of ferrous sulphate-induced oxidation of lipid system were used for characterization of antioxidant activity of extracts. The three extracts showed

Saeedeh Arabshahi-Delouee; Asna Urooj

2007-01-01

23

The antioxidant properties of carnitine in vitro  

Microsoft Academic Search

Many of the effects of carnitine are ascribed to its antioxidant properties. The aim of this study was to evaluate the antioxidant\\u000a properties of carnitine in vitro. Carnitine was found to decolorize ABTS•+, and to protect fluorescein against bleaching induced by AAPH-derived peroxyl radicals and peroxynitrite, thiol groups against\\u000a oxidation induced by hydrogen peroxide, peroxyl radicals, hypochlorite and peroxynitrite, and

Katarzyna Solarska; Anna Lewi?ska; Agata Karowicz-Bili?ska; Grzegorz Bartosz

2010-01-01

24

Antioxidant contents and antioxidative properties of traditional rye breads.  

PubMed

The purpose of this research was to find out the effect of flour extraction rate on the antioxidative properties of traditional rye bread and then to compare the bioactive compounds content and antioxidant properties of rye breads with commercial wheat roll. Four types of rye flour with different extraction rates of 100 (whole meal dark flour), 95 (brown flour), 90 (brown flour), and 70% (light flour) originated from Warko rye cultivar were used for traditional bread baking with sourdough fermentation. Four types of the respective rye breads were analyzed for their potentially beneficial components, including tocopherols and tocotrienols, total phenolics and flavonoids, reduced glutathione, and inositol hexaphosphates. Moreover, the phenolic acids profile was provided. The Trolox equivalent antioxidant capacity (TEAC) of the breads was evaluated using free radical scavenging activities of 80% methanol extracts against ABTS*+ radical cation (ABTS radical cation decolorization method) whereas radical scavenging activity (RSA) was determined against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH*). The superoxide dismutase-like activity (SOD-like activity) was evaluated as free radical scavenging activities of PBS extracts against superoxide anion radicals (O2*-). The results were compared to whole meal rye bread as well as to wheat roll taken as representative example of wheat based bakery product. The studies showed that flour extraction rates strongly affected the content of bioactive compounds and antioxidative properties of traditionally baked rye breads. The incorporation of the rye flours with extraction rates from 100 down to 70% in the formulation caused decrease in tocopherol (T), tocotrienol (T3), inositol hexaphosphate (IP6), and phenolic compound (TPC) contents in rye breads. No changes in reduced glutathione (GSH) contents were noted between each type of rye bread. A significant decrease in Trolox equivalent antioxidant capacity and radical DDPH scavenging activity was also found in bread formulated on flour with an extraction rate of 70% in comparison to the breads formulated on flour with extraction rates from 100 to 90%. The highest SOD-like activity was noted for rye bread formulated on flour with an extraction rate of 70%. The four types of rye breads showed better antioxidative properties and higher antioxidant contents when compared to wheat roll with one exception made to tocopherols and tocotrienols. PMID:17263468

Michalska, Anna; Ceglinska, Alicja; Amarowicz, Ryszard; Piskula, Mariusz Konrad; Szawara-Nowak, Dorota; Zielinski, Henryk

2007-02-01

25

Antioxidant properties of polysaccharides from Ganoderma tsugae  

Microsoft Academic Search

Ganoderma tsugae Murrill (Ganodermataceae) were available in the form of mature and baby Ling chih, mycelia and fermentation filtrate. From these four forms, hot water extracted and hot alkali extracted polysaccharides were prepared and their antioxidant properties were studied. Polysaccharides showed good antioxidant activity as evidenced by their particularly low EC50 values (<0.1mg\\/ml). At 20mg\\/ml, both extracted polysaccharides from mycelia

Yu-Hsiu Tseng; Joan-Hwa Yang; Jeng-Leun Mau

2008-01-01

26

Antioxidant properties of several commercial mushrooms  

Microsoft Academic Search

Winter (strains white and yellow), shiitake (strains 271 and Tainung 1) and oyster mushrooms (abalone and tree oyster mushrooms) were obtained commercially and methanolic extracts were prepared from these mushrooms and their antioxidant properties were studied. The antioxidant activities by the 1,3-diethyl-2-thiobarbituric acid method were moderate to high at 1.2 mg ml?1. Reducing powers were excellent (and higher than 1.28

Joan-Hwa Yang; Hsiu-Ching Lin; Jeng-Leun Mau

2002-01-01

27

[Structure, antioxidative and anticancer properties of flavonoids].  

PubMed

Flavonoids are compounds occuring in plants, e.g. in fruits and vegetables. Flavonoids have been identified as: flavones, flavanones, flavanols (flavan-3-ols), flavonols, anthocyanidines, isoflavonoids and neoflavonoids. Their antioxidative properties are connected with their ability to scavenge free radicals. Their antioxidant properties are linked to the ability to chelate transitional metal ions, mainly copper and iron and to increase antioxidant capacity by the stimulation of the activity of important antioxidant enzymes: superoxide dismutase, glutathione peroxidase and catalase. Flavonoids are able to inhibit the activities of prooxidant enzymes such as cyclooxygenase, lipooxygenase, xanthine oxidase and expression of inducible nitric oxide synthase. These compounds can also regenerate ascorbyl and tocoferoxyl radicals to corresponding vitamins. Pharmacological properties of flavonoids are manifested in different ways. They display antiviral, anti-allergic, anti-inflammatory and anticancer properties. Flavonoids play also a role as inhibitors of neurodegenerative diseases (Alzheimer and Parkinson's diseases) and ageing. Moreover, protective effects against ionizing and UV radiation have been shown for flavonoids. In this paper the antioxidative properties and antitumour action of flavonoids, such as blockade of cell cycle, activation of apoptosis pathways and inhibition of cancerogenesis by inactivation of some carcinogens are reviewed. PMID:23373409

Czapli?ska, Ma?gorzata; Czepas, Jan; Gwo?dzi?ski, Krzysztof

2012-01-01

28

Morphological and genetic characterization of a cultivated Cordyceps sinensis fungus and its polysaccharide component possessing antioxidant property in H22 tumor-bearing mice  

Microsoft Academic Search

Cordyceps sinensis, one of the most precious traditional Chinese medicines, possesses the antitumor activity, antioxidant activity and the capability of modulating the immune system. In the present study, a fungus strain G1 isolated from wild C. sinensis was identified and initially characterized. A phylogenetic tree was generated based on the sequences of the internal transcribed spacer (ITS) region of related

Jiaping Chen; Weiyun Zhang; Tingting Lu; Jing Li; Yi Zheng; Lingdong Kong

2006-01-01

29

Antioxidant properties of Mannich bases.  

PubMed

The biological importance of antioxidants influenced to synthesize some curcumin-related compounds as potential antioxidants. Accordingly, a series of 2,4-diaryl-3-azabicyco[3.3.1]nonan-9-ones were synthesized with polyphenolic and/or polymethoxyphenyl groups by modified Mannich condensations. The yield was significantly improved using BF(3)·SiO(2) as heterogeneous catalyst under mild conditions. Stereochemistry of all the synthesized compounds was established as twin-chair with an equatorial disposition of the aryl groups, through their NMR and XRD interpretations. The ABNs 8 (curcumin analog) and 10 (bis-demethoxycurcumin analog) showed an effective profile over curcumin, ?-tocopherol, and vitamin C by chemical methods. Further, the efficiency of one of the active molecules, ABN 10, was demonstrated by its intracellular ROS inhibition activity on RAW 264.7 macrophage cells by FACS analysis in dose-dependent manner. PMID:22995623

Park, Dong Ho; Venkatesan, Jayachandran; Kim, Se-Kwon; Ramkumar, Venkatachalm; Parthiban, Paramasivam

2012-08-29

30

Impairment of serum albumin antioxidant properties in obstructive sleep apnoea  

Microsoft Academic Search

Antioxidant counteraction of oxidative stress has been poorly explored in obstructive sleep apnoea (OSA). Serum albumin is a major antioxidant agent and structural modifications induced by glucose or free radicals impair its antioxidant properties. The aim of the present study was to compare antioxidant capacities and structural changes of albumin in nonobese OSA patients and healthy volunteers. Albumin structural changes

P. Faure; R. Tamisier; A. Favier; S. Halimi; P. Levy

31

Fungal enhancement of the antioxidant properties of grape waste  

Microsoft Academic Search

The physico-chemical properties of grape waste were characterized to evaluate its potential to support the fungal growth of\\u000a four Aspergillus and three Penicillium strains under solid-state fermentation (SSF) conditions for the production of phenolic antioxidants. In addition, the free\\u000a radical-scavenging potential of DPPH? and ABTS?+ radical solutions and lipid oxidation inhibition by linoleic acid peroxidation of extracts from fermented and

Guillermo Cristian Martínez-Ávila; Antonio F. Aguilera-Carbó; Raúl Rodríguez-Herrera; Cristóbal Noé Aguilar

32

Specific antioxidant properties of human serum albumin.  

PubMed

Human serum albumin (HSA) has been used for a long time as a resuscitation fluid in critically ill patients. It is known to exert several important physiological and pharmacological functions. Among them, the antioxidant properties seem to be of paramount importance as they may be implied in the potential beneficial effects that have been observed in the critical care and hepatological settings. The specific antioxidant functions of the protein are closely related to its structure. Indeed, they are due to its multiple ligand-binding capacities and free radical-trapping properties. The HSA molecule can undergo various structural changes modifying its conformation and hence its binding properties and redox state. Such chemical modifications can occur during bioprocesses and storage conditions of the commercial HSA solutions, resulting in heterogeneous solutions for infusion. In this review, we explore the mechanisms that are responsible for the specific antioxidant properties of HSA in its native form, chemically modified forms, and commercial formulations. To conclude, we discuss the implication of this recent literature for future clinical trials using albumin as a drug and for elucidating the effects of HSA infusion in critically ill patients. PMID:23414610

Taverna, Myriam; Marie, Anne-Lise; Mira, Jean-Paul; Guidet, Bertrand

2013-02-15

33

Antioxidant properties of hydroxy-flavones  

Microsoft Academic Search

The antioxidant properties of 24 hydroxy-flavones were evaluated. Results show that 2?,3?,4?-OH substitution on the B ring plays a crucial role in radical scavenger activity in the DPPH assay and in the inhibitory effect on pereoxydation of tissue lipids in the MDA test. The formation of stable radicals for this type of compounds has been studied by ESR. In addition,

Nicole Cotelle; Jean-Luc Bernier; Jean-Pierre Catteau; Jean Pommery; Jean-Claude Wallet; Emile M. Gaydou

1996-01-01

34

Extensive screening for herbal extracts with potent antioxidant properties  

PubMed Central

This paper summarizes our research for herbal extracts with potent antioxidant activity obtained from a large scale screening based on superoxide radical (O2•?) scavenging activity followed by characterization of antioxidant properties. Firstly, scavenging activity against O2•? was extensively screened from ethanol extracts of approximately 1000 kinds of herbs by applying an electron spin resonance (ESR)-spin trapping method, and we chose four edible herbal extracts with prominently potent ability to scavenge O2•?. They are the extracts from Punica granatum (Peel), Syzygium aromaticum (Bud), Mangifera indica (Kernel), and Phyllanthus emblica (Fruit). These extracts were further examined to determine if they also scavenge hydroxyl radical (•OH), by applying the ESR spin-trapping method, and if they have heat resistance as a desirable characteristic feature. Experiments with the Fenton reaction and photolysis of H2O2 induced by UV irradiation demonstrated that all four extracts have potent ability to directly scavenge •OH. Furthermore, the scavenging activities against O2•? and •OH of the extracts of P. granatum (peel), M. indica (kernel) and P. emblica (fruit) proved to be heat-resistant. The results of the review might give useful information when choosing a potent antioxidant as a foodstuff. For instance, the four herbal extracts chosen from extensive screening possess desirable antioxidant properties. In particular, the extracts of the aforementioned three herbs are expected to be suitable for food processing in which thermal devices are used, because of their heat resistance.

Niwano, Yoshimi; Saito, Keita; Yoshizaki, Fumihiko; Kohno, Masahiro; Ozawa, Toshihiko

2011-01-01

35

Antiglycation and antioxidant properties of soy sauces.  

PubMed

Diabetes-induced hyperglycemia increases formation of advanced glycation end products (AGEs) and metal-catalyzed production of free radicals. This study compared the antioxidant capacities of dark and light soy sauces of different brands and investigated their abilities to inhibit AGEs and whether their mechanism of action was pre- or post-Amadori or involved chelation of transition metals. The antioxidant capacities of soy sauces were compared using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) method and by measuring their total phenolic contents. Model proteins (lysozyme, albumin) were glycated using fructose with or without soy sauces with subsequent analysis of cross-linked AGEs by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The effect of soy sauces on pre- and post-Amadori inhibition of AGEs was investigated by measuring fructosamine and AGEs following reincubation of ribose-glycated (ribated) lysozyme, respectively. Dark soy sauces had higher antioxidant capacities and phenolic content and were more effective inhibitors of post-Amadori-derived cross-linked AGEs. However, light soy sauces were more effective at inhibiting fructosamine and had more potent metal chelation properties. This study reports the antiglycation properties of soy sauces, but further studies are required to determine the constituents responsible for this effect and whether soy sauce consumption can reduce oxidative stress and AGEs in diabetic subjects. PMID:21861728

Mashilipa, Changwe; Wang, Qiuyu; Slevin, Mark; Ahmed, Nessar

2011-08-23

36

ANTIOXIDANT AND ANTICANCER PROPERTIES OF BERRY FRUITS  

Technology Transfer Automated Retrieval System (TEKTRAN)

Berry fruits are considered excellent functional foods because they contain high levels of natural antioxidants. Antioxidants can act as free radical scavengers, peroxide decomposers, singlet and triplet oxygen quenchers, enzyme inhibitors, and synergists. Therefore, antioxidants can delay or prev...

37

Olea europaea L. leaf extract and derivatives: antioxidant properties.  

PubMed

This paper reports a very simple and fast method to collect eluates with high amounts of hydroxytyrosol, biotransforming Olea europaea L. leaf extract by a thermophilic beta-glycosidase immobilized on chitosan. Some phenolic compounds in the leaf tissue and in the eluates obtained by biotransformation are identified. To propose the eluates as natural substances from a vegetal source, their antioxidant properties have been compared with those of the leaf extract from which they are originated. The eluates possess a higher concentration of simple phenols, characterized by a stronger antioxidant capacity, than those available in extra virgin olive oils and in many tablets of olive leaf extracts, commercially found as dietetic products and food integrators. PMID:12166985

Briante, Raffaella; Patumi, Maurizio; Terenziani, Stefano; Bismuto, Ettore; Febbraio, Ferdinando; Nucci, Roberto

2002-08-14

38

Antioxidant properties of the fatty alcohol esters of gallic acid  

Microsoft Academic Search

Summary and Conclusions  The antioxidant properties of octyl, dodecyl, tetradecyl, hexadecyl, and octadecyl gallates in lard substrate were determined\\u000a by the active oxygen method. The order of their effectiveness was about the same as that of the more active antioxidants,\\u000a nordihydroguariaretic acid and gallic acid, that have been reported. The carry-over of the antioxidant properties into baked\\u000a goods was determined by

Steward G. Morris; Lillian A. Kraekel; Dorothy Hammer; J. S. Myers; R. W. Riemenschneider

1947-01-01

39

[Antioxidant properties of a series of extracts from medicinal plants].  

PubMed

Investigation of antioxidant properties of some plants was carried out. A group of plants affected human central nervous system was studied in detail. Efficiency of plants as antioxidants was tested by the influence of their extracts on the yield of photochemiluminescence of Gly-Trp solutions. Antioxidant properties were examined under conditions when their own absorption was minimized. Riboflavin as additional sensitizer was used in this experiment for superoxide generation. The antioxidant effect was evaluated with regard to single dose of plant extracts and their concentration in human organism. The effect decreases in the following consequence: Hypericum > Eleutherococcus > Rhodiola > Leonurus > Aralia > Valeriana > Echinopanax > Schizandra > Panax gin-seng. PMID:9172694

Bol'shakova, I V; Lozovskaia, E L; Sapezhinski?, I I

40

In vitro antioxidant properties of morphine  

Microsoft Academic Search

Morphine is implicated in diverse functions, from development to immune modulation in the central and peripheral nervous systems. It has also been used extensively in the clinical management of pain due to its potent analgesic effect. This study was designed to evaluate the in vitro antioxidant capacity of morphine using different antioxidant tests, including total antioxidant activity, reducing power, free

?lhami Gülç?n; ?ükrü Beydem?r; H. Ahmet Alici; Mahfuz Elmasta?; M. Emin Büyükokuro?lu

2004-01-01

41

Human Meconium Has Potent Antioxidative Properties  

Microsoft Academic Search

In vitro the antioxidative capacity of pooled and lyophilized human meconium, measured by chemiluminescence, was compared to that of three potent antioxidants: vitamin C, a vitamin E analogue and a synthetic antioxidant, butylated hydroxytoluene. Meconium showed a significant superoxide trapping and peroxidation prevention capacity, but its capacity to trap peroxyl radicals was minor. These effects of meconium were possibly due

Pekka Kääpä; Jaakko Kytölä; Hanna Soukka; Markku Ahotupa

1997-01-01

42

[Antioxidant properties of benzofurocaine, phenycaberan and orthophen].  

PubMed

The antioxidant properties of benzofurocaine, phenycaberan, crithophen, alpha-tocopherol and ionol were evaluated by inhibition of oxygen absorption by liposomes from egg phosphatidylcholine induced by the addition of an prooxidant. The activity of the tested agents at inhibition of Fe2+-ascorbater-initiated oxidation decreases in the order: ionol, benzofurocaine, phenycaberan, orthophen, alpha-tocopherol; emine-initiated (EDTA-independent) oxidation of phosphatidylcholine is inhibited only by ionol, orthophen and alpha-tocopherol. Phenycaberan exerts no effect on oxidation and benzofurocaine increases the rate of emine-initiated absorption of oxygen. Thus, benzofurocaine, phenycaberan and orthophen may be referred to as selective inhibitors of Fe2+-initiated peroxidation of phosphatidylcholine. PMID:2792356

Lebedev, A V; Kuz'min, A V; Levitski?, D O; Stepaniuk, G I

43

Chemical properties and antioxidant and antimicrobial activities of Slovenian propolis.  

PubMed

The chemical composition as well as the antioxidant and antimicrobial activities of two EtOH extracts of propolis (PEEs) from Slovenia were determined. EtOH was used as extracting solvent at 70 and 96%, providing the extracts PEE70 and PEE96, respectively. The extraction with 70% EtOH was more efficient than that with 96% EtOH, as the PEE70 was richer in total phenolic compounds than the PEE96. The Slovenian propolis was characterized by different phenolic acids and flavonoids. The PEE96 was slightly richer in three specific compounds, i.e., caffeic acid, ferulic acid, and luteolin, while all other substances detected showed higher contents in the PEE70. The PEE70 showed a stronger reducing power and ability to scavenge free radicals and metal ions than the PEE96. Both PEEs were in the main more effective against Gram-positive bacteria than against fungi and Gram-negative bacteria like Salmonella and Escherichia coli, with the exception of Campylobacter. The PEE96 decreased the intracellular oxidation in Saccharomyces cerevisiae in a dose-dependent manner. The antimicrobial activities and antioxidant properties were related to the total phenolic contents. The two PEEs have the potential for use as natural antimicrobial and antioxidant additives in foods. PMID:22899615

Mavri, Ana; Abramovi?, Helena; Polak, Tomaž; Bertoncelj, Jasna; Jamnik, Polona; Smole Možina, Sonja; Jeršek, Barbara

2012-08-01

44

Antioxidant and antimutagenic properties of Hibiscus tiliaceus L. methanolic extract.  

PubMed

The genus Hibiscus thrives in a variety of climates and produces a diversity of natural compounds with bioactive properties. We have studied the chemical composition and the in vivo antioxidant properties of Hibiscus tiliaceus L. methanolic flower extract, as well as its mutagenic/antimutagenic effects. Vitamin E and some stigmasterol derivatives that might confer an antioxidant effect to the extract were present. Treatment with this extract protected several Saccharomyces cerevisiae strains defective in antioxidant defenses against H2O2 and t-BOOH cytotoxicities, showing a clear antioxidant activity. The effect is the same for all strains used, independent of the antioxidant defense disrupted, suggesting that protection may be due to molecules that act as versatile and wide spectrum nonenzymatic antioxidants, such as vitamins or phytosterols. The extract was not mutagenic in either Salmonella typhimurium or S. cerevisiae and showed a significant antimutagenic action against oxidative mutagens in S. cerevisiae. PMID:16968101

Rosa, Renato Moreira; Melecchi, Maria Inés S; da Costa Halmenschlager, Rafael; Abad, Fernanda C; Simoni, Cristina Rosat; Caramão, Elina B; Henriques, João Antonio Pêgas; Saffi, Jenifer; de Paula Ramos, Ana Lígia Lia

2006-09-20

45

Reinvestigation of the antioxidant properties of conjugated linoleic acid  

Microsoft Academic Search

Despite repeated suggestions that antioxidant activity of conjugated linoleic acid (CLA), a collective of conjugated dienoic\\u000a isomers of linoleic acid, underlies its reported anticarcinogenic and antiatherosclerotic effects, the antioxidant properties\\u000a of CLA remain ill-defined. Therefore, this study was undertaken to gain more insight into the mechanism of potential CLA antioxidant\\u000a activity. It was tested whether CLA could protect membranes composed

Nancy E. Cook; Diane L. Tribble

1995-01-01

46

Antioxidant properties of bucillamine: Possible mode of action  

Microsoft Academic Search

The antioxidant properties of Bucillamine (BUC), a di-thiol compound used for treatment of rheumatoid arthritis (RA) and its possible mode of action, were investigated. BUC exhibits potent antioxidant activity similar to those of trolox and ascorbic acid. It reduces the stable free radical diphenyl-2-picrylhydrazyl (DPPH) with IC50 of 18.5±0.1?mol, its relative antioxidant activity by the ferric reducing ability (FRAP) is

Dalia Mazor; Liya Greenberg; Dror Shamir; Dan Meyerstein; Naomi Meyerstein

2006-01-01

47

Antioxidant properties of minocycline: neuroprotection in an oxidative stress assay and direct radical-scavenging activity.  

PubMed

Minocycline is neuroprotective in animal models of a number of acute CNS injuries and neurodegenerative diseases. While anti-inflammatory and anti-apoptotic effects of minocycline have been characterized, the molecular basis for the neuroprotective effects of minocycline remains unclear. We report here that minocycline and a number of antioxidant compounds protect mixed neuronal cultures in an oxidative stress assay. To evaluate the role of minocycline's direct antioxidant properties in neuroprotection, we determined potencies for minocycline, other tetracycline antibiotics, and reference antioxidant compounds using a panel of in vitro radical scavenging assays. Data from in vitro rat brain homogenate lipid peroxidation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays show that minocycline, in contrast to tetracycline, is an effective antioxidant with radical scavenging potency similar to vitamin E. Our findings suggest that the direct antioxidant activity of minocycline may contribute to its neuroprotective effects in some cell-based assays and animal models of neuronal injury. PMID:16033424

Kraus, Richard L; Pasieczny, Rodger; Lariosa-Willingham, Karen; Turner, Mary S; Jiang, Alice; Trauger, John W

2005-08-01

48

[Antioxidant properties of novel preparations--bioflavonoid derivatives and tannins].  

PubMed

New medicinal plant preparations of polyphenol nature, representing the derivatives of bioflavonoids (piflamin) and ellagotannins (altan and ellagic acid) were experimentally studied. The drugs exhibited antioxidant properties, which were manifested by inhibition of a pathological lipid peroxidation, restoration of the functional activity of the antioxidant system components, and stabilization of the hepatocyte membranes. PMID:11548450

Iakovleva, L V; Gerasimova, O A; Karbusheva, I V; Ivakhnenko, A K; Buniatian, N D; Sakharova, T S

49

Evaluation of antioxidant properties of berries  

Microsoft Academic Search

The present paper focuses on assessing the levels of various enzymatic and non-enzymatic antioxidants in selected berries\\u000a of Indian sub-continent viz., gooseberry, grapes, orange and tomato. This study has revealed that orange, tomato and grapes\\u000a possess predominant quantities of enzymatic antioxidants namely SOD, catalase and glutathione peroxidase respectively. The\\u000a levels of antioxidants analyzed namely reduced glutathione, vitamin C and vitamin

P. Rani; K. Meena Unni; J. Karthikeyan

2004-01-01

50

Antioxidant and radical scavenging properties of curcumin  

Microsoft Academic Search

Curcumin (diferuoyl methane) is a phenolic compound and a major component of Curcuma longa L. In the present paper, we determined the antioxidant activity of curcumin by employing various in vitro antioxidant assays such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2?-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total

Tuba Ak; ?lhami Gülçin

2008-01-01

51

Preliminary structural characterization and antioxidant activities of polysaccharides extracted from Hawk tea (Litsea coreana var. lanuginosa).  

PubMed

Three polysaccharides were extracted from different leaf age Hawk teas (Litsea coreana var. lanuginosa) by hot water method. Preliminary structural characterization was conducted by physicochemical property, fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analyses. Antioxidant activities against 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ferric reducing activity power (FRAP), hydroxyl radical and erythrocyte hemolysis were also evaluated. The physicochemical property analysis indicated significant differences in the three polysaccharides. The FTIR spectra revealed the general characteristic absorption peaks of the three polysaccharides. The SEM images demonstrated significant differences in the surface features of the different polysaccharides. The antioxidant activity assays revealed the obvious antioxidant activities of three polysaccharides, and the polysaccharides of Hawk primary leaf tea exhibited higher antioxidant activities than the other two polysaccharides. With current findings, the polysaccharides from Hawk primary leaf tea may have potential applications in food industries. PMID:23618259

Jia, Xuejing; Dong, Lihua; Yang, Yong; Yuan, Shu; Zhang, Zhongwei; Yuan, Ming

2013-03-13

52

Antioxidant Properties of Pyrrolobenzimidazole Derivative RU-792: Experimental Study.  

PubMed

We studied antioxidant activity of new derivative of pyrrolo[1,2-?]benzimidazole RU-792 and compared its effects on free radical processes with those of the reference antioxidant Trolox in four model free-radical systems. RU-792 had high antioxidant activity determined by its intrinsic antiradical properties. RU-792 was superior to Trolox by antioxidant activity in the models of Fe(2+)-induced chemiluminescence of lipids with 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), but less effective in the model of luminol-dependent chemiluminescence. PMID:24143368

Kosolapov, V A; Eltsova, L V; Spasov, A A; Anisimova, V A

2013-07-01

53

Antioxidant properties of some lichen species.  

PubMed

Antioxidant activity of the acetone, methanol and aqueous extracts of the lichens Cladonia furcata, Hypogymnia physodes, Lasallia pustulata, Parmelia caperata and Parmelia sulcata has been screened in vitro by using different methods (DPPH radical scavenging, superoxide anion radical scavenging, reducing power, determination of total phenolic compounds and determination of total flavonoid content). Of the lichens tested, Lasallia pustulata had powerful antioxidant activities. Acetone, methanol and aqueous extracts of this lichen showed 90.93, 69.87 and 65.08% DPPH radical scavenging activities. Moreover, the tested extracts had effective reducing power and superoxide anion radical scavenging. Those various antioxidant activities were compared to standard antioxidants such as ascorbic acid, butylated hydroxyanisole (BHA) and ?-tocopherol. In addition, total content of phenol and flavonoid in extracts were determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The strong relationships between total phenolic and flavonoid contents and the antioxidative activities of tested extracts suggest that these compounds play important role in antioxidant activity. The present stady shows that tested lichen species demonstrated a strong antioxidant activity and can be considered as good sources of natural antioxidants. PMID:23572791

Kosani?, Marijana; Rankovi?, Branislav; Vukojevi?, Jelena

2010-12-22

54

Antioxidant properties of anesthetics: the biochemist, the surgeon and the anesthetist.  

PubMed

General anesthesia can impair immunological defense mechanisms while inducing an inflammatory reaction. Generalized inflammatory reactions involve leucocytes which in turn release inflammatory mediators and free oxygen radicals. General anesthetics include a series of gaseous and intravenous sedative-hypnotic agents indicated for induction and maintenance of general anesthesia as well as for sedation of intubated, mechanically ventilated adults in intensive care units (ICU). Some anesthetics, such as propofol, are characterized by a phenolic structure similar to that of alpha-tocopherol, and exhibit antioxidant properties that have been demonstrated both in vitro and in vivo. Similarly, other anesthetics show antioxidant and protective roles but this mechanism is to be related to their ability to induce antioxidant enzyme (i.e., heme oxygenase-1). The aim of the present review is to evaluate the antioxidant properties of anesthetics in various experimental models and if they may be considered efficient therapeutic tools in counteracting oxidative stress during general anesthesia and sedation in ICU. PMID:19169610

Li Volti, G; Basile, F; Murabito, P; Galvano, F; Di Giacomo, C; Gazzolo, D; Vadalà, S; Azzolina, R; D'Orazio, N; Mufeed, H; Vanella, L; Nicolosi, A; Basile, G; Biondi, A

55

Physico-chemical and antioxidant properties of four mango (Mangifera indica L.) cultivars in China.  

PubMed

Four principal mango cultivars (Tainong No.1, Irwin, JinHwang and Keitt) grown in southern China were selected, and their physico-chemical and antioxidant properties were characterized and compared. Of all the four cultivars, Tainong No.1 had highest content of total phenols, ?-coumaric acid, sinapic acid, quercetin, titratable acidity, citric acid, malic acid, fructose, higher antioxidant activities (DPPH, FRAP) and L(*), lower pH, PPO activity and individual weight. Keitt mangoes showed significantly (p<0.05) higher contents of ?-carotene, ?-hydroxybenzoic acid, sucrose, total sugar, total soluble solid, catechin, succinic acid and higher PPO activity. JinHwang mangoes exhibited significantly (p<0.05) higher individual weight and PPO activity, but had lower content of total phenols, ?-carotene and lower antioxidant activity. Principal component analysis (PCA) allowed the four mango cultivars to be differentiated clearly based on all these physico-chemical and antioxidant properties determined in the study. PMID:23265504

Liu, Feng-Xia; Fu, Shu-Fang; Bi, Xiu-Fang; Chen, Fang; Liao, Xiao-Jun; Hu, Xiao-Song; Wu, Ji-Hong

2012-11-08

56

Cysteine-containing peptides having antioxidant properties  

Microsoft Academic Search

Cysteine containing amphipathic alpha helices of the exchangeable apolipoproteins, as exemplified by apolipoprotein (apo) A-I.sub.Milano (R173C) and apoA-I.sub.Paris, (R151C) were found to exhibit potent antioxidant activity on phospholipid surfaces. The addition of a free thiol, at the hydrophobic\\/hydrophilic interface of an amphipathic alpha helix of synthetic peptides that mimic HDL-related proteins, imparts a unique antioxidant activity to these peptides which

Bielicki; John K

2009-01-01

57

Cysteine-containing peptides having antioxidant properties  

Microsoft Academic Search

Cysteine containing amphipathic alpha helices of the exchangeable apolipoproteins, as exemplified by apolipoprotein (apo) A-I.sub.Milano (R173C) and apoA-I.sub.Paris, (R151C) were found to exhibit potent antioxidant activity on phospholipid surfaces. The addition of a free thiol, at the hydrophobic\\/hydrophilic interface of an amphipathic alpha helix of synthetic peptides that mimic HDL-related proteins, imparts a unique antioxidant activity to these peptides which

Bielicki; John K

2008-01-01

58

Antioxidant properties of kilned and roasted malts.  

PubMed

Compounds possessing antioxidant activity play a crucial role in delaying or preventing lipid oxidation in foods and beverages during processing and storage. Such reactions lead to loss of product quality, especially as a consequence of off-flavor formation. The aim of this study was to determine the antioxidant activity of kilned (standard) and roasted (speciality) malts in relation to phenolic compounds, sugars, amino acids, and color [assessed as European Brewing Convention units (degrees EBC) and absorbance at 420 nm]. The concentrations of sugars and amino acids decreased with the intensity of the applied heat treatment, and this was attributed to the extent of the Maillard reaction, as well as sugar caramelization, in the highly roasted malts. Proline, followed by glutamine, was the most abundant free amino/imino acid in the malt samples, except those that were highly roasted, and maltose was the most abundant sugar in all malts. Levels of total phenolic compounds decreased with heat treatment. Catechin and ferulic acid were the most abundant phenolic compounds in the majority of the malts, and amounts were highest in the kilned samples. In highly roasted malts, degradation products of ferulic acid were identified. Antioxidant activity increased with the intensity of heating, in parallel with color formation, and was significantly higher for roasted malts compared to kilned malts. In kilned malts, phenolic compounds were the main identified contributors to antioxidant activity, with Maillard reaction products also playing a role. In roasted malts, Maillard reaction products were responsible for the majority of the antioxidant activity. PMID:16190672

Samaras, Thomas S; Camburn, Philip A; Chandra, Sachin X; Gordon, Michael H; Ames, Jennifer M

2005-10-01

59

Antioxidant properties of hot water extracts from Ganoderma tsugae Murrill  

Microsoft Academic Search

Ganoderma tsugae Murrill (Ganodermataceae) were available in the form of mature and baby Ling chih, mycelia and fermentation filtrate. From these four forms, hot water extracts were prepared and their antioxidant properties were studied. Hot water extracts from mature and baby Ling chih showed high antioxidant activities (78.5% and 78.2%) at 20mg\\/ml, and had EC50 values of 7.25 and 5.89mgextract\\/ml,

Jeng-Leun Mau; Shu-Yao Tsai; Yu-Hsiu Tseng; Shih-Jeng Huang

2005-01-01

60

Cysteine-containing peptides having antioxidant properties  

DOEpatents

Cysteine containing amphipathic alpha helices of the exchangeable apolipoproteins, as exemplified by apolipoprotein (apo) A-I.sub.Milano (R173C) and apoA-I.sub.Paris, (R151C) were found to exhibit potent antioxidant activity on phospholipid surfaces. The addition of a free thiol, at the hydrophobic/hydrophilic interface of an amphipathic alpha helix of synthetic peptides that mimic HDL-related proteins, imparts a unique antioxidant activity to these peptides which inhibits lipid peroxidation and protects phospholipids from water-soluble free radical initiators. These peptides can be used as therapeutic agents to combat cardiovascular disease, ischemia, bone disease and other inflammatory related diseases.

Bielicki, John K. (Castro Valley, CA)

2008-10-21

61

Cysteine-containing peptides having antioxidant properties  

SciTech Connect

Cysteine containing amphipathic alpha helices of the exchangeable apolipoproteins, as exemplified by apolipoprotein (apo) A-I.sub.Milano (R173C) and apoA-I.sub.Paris, (R151C) were found to exhibit potent antioxidant activity on phospholipid surfaces. The addition of a free thiol, at the hydrophobic/hydrophilic interface of an amphipathic alpha helix of synthetic peptides that mimic HDL-related proteins, imparts a unique antioxidant activity to these peptides which inhibits lipid peroxidation and protects phospholipids from water-soluble free radical initiators. These peptides can be used as therapeutic agents to combat cardiovascular disease, ischemia, bone disease and other inflammatory related diseases.

Bielicki, John K. (Castro Valley, CA)

2009-10-13

62

Design and synthesis of new hypocholesterolemic organosilanes with antioxidant properties  

Microsoft Academic Search

(Arylamino)methylsilane derivatives 3 have been prepared and identified as potent inhibitors of human LDL oxidation mediated by copper(II). Combination of this property with the structural requirement of squalene epoxidase inhibitors led to the design and synthesis of the (arylamino)methylsilane derivative 4 which was characterised as the first potent, orally active squalene epoxidase inhibitor with antioxidant properties.

J.-P. Gotteland; A. Delhon; D. Junquéro; P. Oms; S. Halazy

1996-01-01

63

The antioxidant properties of different phthalocyanines.  

PubMed

Oxidative stress is involved in the etiology of several chronic diseases, including cardiovascular disease, diabetes, cancer, and neurodegenerative disorders. From this perspective, we have evaluated the possible antioxidant capacities of five different phthalocyanines (PCs), consisting of four metallophthalocyanines (MPCs) and one simple phthalocyanine (PC) in order to explore, for the first time, the potential antioxidant activities of these compounds. Our results show that all PCs tested in this study have significant antioxidant activity in lipid peroxidation assay, providing protection from sodium nitroprusside -induced oxidative damage to supernatant from the homogenized liver, brain, e rim of mice. Compared to the non-induced control, the PCs were generally more efficient in reducing malondialdehyde levels in all assays on lipid peroxidation induced by sodium nitroprusside; the order of approximate decrease in efficiency was as follows: manganese-PC (better efficiency)>copper-PC>iron-PC>zinc-PC>PC (worst efficiency). Furthermore, the copper-PC and manganese-PC compounds exerted a significant protective effect in deoxyribose degradation assays, when employing Fe(2+), Fe(2+)+H(2)O(2), and H(2)O(2) solutions. In conclusion, all PCs tested here were shown to be promising compounds for future in vivo investigations, because of their potential antioxidant activities in vitro. PMID:22024102

Amaral, Guilherme Pires; Puntel, Gustavo Orione; Dalla Corte, Cristiane Lenz; Dobrachinski, Fernando; Barcelos, Rômulo Pillon; Bastos, Luiza Lena; Avila, Daiana Silva; Rocha, João Batista Teixeira; da Silva, Edegar Ozorio; Puntel, Robson Luiz; Soares, Félix Alexandre Antunes

2011-10-17

64

Extracts of Halenia elliptica exhibit antioxidant properties in vitro and in vivo  

Microsoft Academic Search

The antioxidant properties of different extracts of Halenia elliptica was investigated by employing various established in vitro systems. The results showed that various extracts possessed strong antioxidant activity in vitro, and the 70% methanol extract (ME) had the strongest antioxidant activity. Based on our in vitro results, ME was used for investigating the antioxidant properties of H. ellipticain vivo. The

Bo Huang; Haibin Ke; Jingsheng He; Xiaoquan Ban; Hong Zeng; Youwei Wang

2011-01-01

65

Antioxidant characterization of some Sicilian edible wild greens.  

PubMed

Epidemiological studies have demonstrated that many antioxidants and the total antioxidant capacity (TAC) of the diet may protect against cancers and cardiovascular disease. Common fruits and vegetables are good sources of antioxidants, although in some Mediterranean areas traditional wild greens are responsible for a significant percentage of total dietary antioxidant intake. In the European Prospective Investigation into Cancer and Nutrition cohort of Ragusa (Sicily), a high number of subjects were found to frequently eat wild greens, including Sinapis incana and Sinapis nigra, Diplotaxis erucoides, Cichorium intybus, Asparagus acutifolius, and Borrago officinalis. On the basis of these observations, detailed characterization of single antioxidant components (i.e., polyphenols, carotenoids, chlorophylls, and ascorbic acid) and the TAC of these edible wild traditional plants was performed. The wild plants examined were found to be very rich in antioxidants, such as flavonoids and carotenoids, with high TAC values, suggesting that the importance of these vegetables, not only in the traditional but even in the contemporary diet, needs to be emphasized. PMID:16302763

Salvatore, Sara; Pellegrini, Nicoletta; Brenna, Oreste V; Del Rio, Daniele; Frasca, Graziella; Brighenti, Furio; Tumino, Rosario

2005-11-30

66

Antioxidant properties of captopril in vitro.  

PubMed

This paper reports on the effect of captopril on the stability of unsaturated fatty acids in the evening primrose oil. The experiment was performed for captopril at concentrations of 0.05%, 0.1% and 0.2% in the samples of evening primrose oil at temperatures of 20 degrees, 40 degrees and 60 degrees C. The determination was performed on the 10th day of incubation. The results were compared to those obtained for analogous samples with octyl gallate, an antioxidant commonly used in oil systems, taken at the same concentrations and at the same temperatures. The changes in concentrations of the substances studied with time were monitored by gas chromatography. The results have shown captopril to be a stronger antioxidant than octyl gallate at all concentrations and temperatures used. PMID:14556487

Radomska, Anna

67

Synthesis and antioxidant properties of dendritic polyphenols  

Microsoft Academic Search

Three dendritic polyphenols (generation 1) were synthesized: a syringaldehyde-based dendrimer (1), a vanillin-based dendrimer (2), and an iodinated vanillin-based dendrimer (3). They all showed strong antioxidant activity according to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay. The syringaldehyde dendrimer was twice and 10 times stronger than quercetin and Trolox, respectively. The vanillin-based dendrimer and its more hydrophobic iodinated derivative were also more

Choon Young Lee; Ajit Sharma; Jae Eun Cheong; Julie L. Nelson

2009-01-01

68

Ferulic Acid: therapeutic potential through its antioxidant property.  

PubMed

There has been considerable public and scientific interest in the use of phytochemicals derived from dietary components to combat human diseases. They are naturally occurring substances found in plants. Ferulic acid (FA) is a phytochemical commonly found in fruits and vegetables such as tomatoes, sweet corn and rice bran. It arises from metabolism of phenylalanine and tyrosine by Shikimate pathway in plants. It exhibits a wide range of therapeutic effects against various diseases like cancer, diabetes, cardiovascular and neurodegenerative. A wide spectrum of beneficial activity for human health has been advocated for this phenolic compound, at least in part, because of its strong antioxidant activity. FA, a phenolic compound is a strong membrane antioxidant and known to positively affect human health. FA is an effective scavenger of free radicals and it has been approved in certain countries as food additive to prevent lipid peroxidation. It effectively scavenges superoxide anion radical and inhibits the lipid peroxidation. It possesses antioxidant property by virtue of its phenolic hydroxyl group in its structure. The hydroxy and phenoxy groups of FA donate electrons to quench the free radicals. The phenolic radical in turn forms a quinone methide intermediate, which is excreted via the bile. The past few decades have been devoted to intense research on antioxidant property of FA. So, the present review deals with the mechanism of antioxidant property of FA and its possible role in therapeutic usage against various diseases. PMID:18188410

Srinivasan, Marimuthu; Sudheer, Adluri R; Menon, Venugopal P

2007-03-01

69

Characterization and antioxidant activity of dihydromyricetin–lecithin complex  

Microsoft Academic Search

Dihydromyricetin is a natural antioxidant with good prospects in food industry. But its poor lipophilic property limits its\\u000a application in lipophilic food. In this study, the dihydromyricetin–lecithin complex was prepared to improve the hydrophobicity\\u000a of dihydromyricetin. The physicochemical properties of the complex were analyzed by ultraviolet–visible spectrometry, infrared\\u000a spectrometry, scanning electron microscopy, differential scanning calorimetry, X-ray diffractometry. The result showed

Benguo LiuJiquan; Jiquan Du; Jie Zeng; Chungang Chen; Shengyang Niu

2009-01-01

70

Direct evidence of caeruloplasmin antioxidant properties.  

PubMed

The chain-breaking antioxidant potential of caeruloplasmin and bovine serum albumin (BSA) has been investigated in comparison with other well-established antioxidants. Their Oxygen Radical Absorbing Capacity (ORAC), was measured by using beta-phycocyanin (beta-PC) as a fluorescent indicator protein, 2,2'-azobis (2-amidinopropane) hydrochloride (AAPH) as a peroxyl radical generator and the water soluble vitamin E analogue, Trolox, as a reference standard. The relative peroxyl absorbing capacities/mole for Trolox, caeruloplasmin, heat-denatured caeruloplasmin (hCP), catalase, bovine serum albumin (BSA), superoxide dismutase (SOD), and deferoxamine were 1; 2.6; 3.3; 3.7; 1.2; 0.1; 0.2, respectively. Caeruloplasmin was far more effective as a peroxyl radical scavenger than SOD, deferoxamine and BSA, but slightly less effective than catalase. The peroxyl radical absorbing capacity of caeruloplasmin was enhanced by heat-denaturation of the protein. Electron paramagnetic resonance (EPR) spectroscopy using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin-trap, was applied in order to measure the scavenger abilities of caeruloplasmin on superoxide radical and hydroxyl radical production and the concentration required to inhibit by 50% oxygen free radical formation (IC50) was determined. The IC50 values of caeruloplasmin, hCP, and BSA for the superoxide radical were 12, 2, 260 microM and for the hydroxyl radical 15, 2, 200 microM. These results show that caeruloplasmin is an effective chain-breaking antioxidant for a variety of radicals, independently of its catalytic ferroxidase activity. PMID:9879663

Atanasiu, R L; Stea, D; Mateescu, M A; Vergely, C; Dalloz, F; Briot, F; Maupoil, V; Nadeau, R; Rochette, L

1998-12-01

71

Antioxidant properties of a North American ginseng extract  

Microsoft Academic Search

A North American ginseng extract (NAGE) containing known principle ginsenosides for Panax quinquefolius was assayed for metal chelation, affinity to scavenge DPPH-stable free radical, and peroxyl (LOO·) and hydroxyl (·OH) free radicals for the purpose of characterizing mechanisms of antioxidant activity. Dissociation constants (Kd) for NAGE to bind transition metals were in the order of Fe2+ > Cu2+ > Fe3+

David D. Kitts; Arosha N. Wijewickreme; Chun Hu

2000-01-01

72

Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo).  

PubMed

Berries contain significant amounts of phytochemicals, including polyphenols, which are reported to reduce cancer risk, coronary heart disease and other degenerative diseases. These effects are mainly attributed to the antioxidant capacity of polyphenols found in berries. Strawberry tree (Arbutus unedo) berries are used in folk medicine but seldom eaten as fresh fruits. Their phenolic profile and antioxidant capacity reveal a high potential, but they are not well characterized as a "health promoting food". The aim of this study was to assess the antioxidant properties of the edible strawberry tree fruit in vitro and in a neurodegeneration cell model. Raspberry (Rubus idaeus), a well documented health-promoting fruit, was used as a control for comparison purposes. A. unedo yielded a similar content in polyphenols and a slightly lower value of total antioxidant capacity in comparison to R. idaeus. Although the chemically-measured antioxidant activity was similar between both fruits, R. idaeus increased neuroblastoma survival in a neurodegeneration cell model by 36.6% whereas A. unedo extracts caused no effect on neuroblastoma viability. These results clearly demonstrate that a promising level of chemically-determined antioxidant activity of a plant extract is not necessarily correlated with biological significance, as assessed by the effect of A. unedo fruit in a neurodegeneration cell model. PMID:22254017

Fortalezas, Sofia; Tavares, Lucélia; Pimpão, Rui; Tyagi, Meenu; Pontes, Vera; Alves, Paula M; McDougall, Gordon; Stewart, Derek; Ferreira, Ricardo B; Santos, Cláudia N

2010-02-21

73

Antioxidant Properties and Neuroprotective Capacity of Strawberry Tree Fruit (Arbutus unedo)  

PubMed Central

Berries contain significant amounts of phytochemicals, including polyphenols, which are reported to reduce cancer risk, coronary heart disease and other degenerative diseases. These effects are mainly attributed to the antioxidant capacity of polyphenols found in berries. Strawberry tree (Arbutus unedo) berries are used in folk medicine but seldom eaten as fresh fruits. Their phenolic profile and antioxidant capacity reveal a high potential, but they are not well characterized as a “health promoting food”. The aim of this study was to assess the antioxidant properties of the edible strawberry tree fruit in vitro and in a neurodegeneration cell model. Raspberry (Rubus idaeus), a well documented health-promoting fruit, was used as a control for comparison purposes. A. unedo yielded a similar content in polyphenols and a slightly lower value of total antioxidant capacity in comparison to R. idaeus. Although the chemically-measured antioxidant activity was similar between both fruits, R. idaeus increased neuroblastoma survival in a neurodegeneration cell model by 36.6% whereas A. unedo extracts caused no effect on neuroblastoma viability. These results clearly demonstrate that a promising level of chemically-determined antioxidant activity of a plant extract is not necessarily correlated with biological significance, as assessed by the effect of A. unedo fruit in a neurodegeneration cell model.

Fortalezas, Sofia; Tavares, Lucelia; Pimpao, Rui; Tyagi, Meenu; Pontes, Vera; Alves, Paula M.; McDougall, Gordon; Stewart, Derek; Ferreira, Ricardo B.; Santos, Claudia N.

2010-01-01

74

In vitro pro- and antioxidant properties of estrogens  

Microsoft Academic Search

The pro- and antioxidant properties of estrogens are subject of debate. The apparent discrepancy is largely caused by the chemical heterogeneity in the estrogen family and by their concentration and the environment in which they are found. To gain some insight into this debate, we determined whether estradiol (E2), estrone (E1), the 2-, 4- and 16?-hydroxyestrogens and also the 2-

Paul A Thibodeau; Rémy Kachadourian; Rosalie Lemay; Martine Bisson; Brian J Day; Benoit Paquette

2002-01-01

75

In vivo and in vitro antioxidant properties of furosemide  

Microsoft Academic Search

The aim of this study was to investigate in vivo and in vitro antioxidant properties of furosemide. In vitro, human red blood cells were submitted to oxidative stress (AAPH), in absence or in presence of different concentrations of furosemide. Potassium efflux was measured in order to quantify the oxidative stress after the action of AAPH on red blood cells. Allophycocyanin

J. J. Lahet; F. Lenfant; C. Courderot-Masuyer; E. Ecarnot-Laubriet; C. Vergely; M. J. Durnet-Archeray; M. Freysz; L. Rochette

2003-01-01

76

Investigation of antioxidant property of iron oxide particlesby 1'-1'diphenylpicryl-hydrazyle (DPPH) method  

Microsoft Academic Search

We investigated the antioxidant properties of iron oxide particles. Particles of different sizes were synthesized and their antioxidant potency was investigated by using an in vitro system. The antioxidant capacity assay was done via a modified DPPH method for insoluble solid materials. The percentages of DPPH scavenging were calculated for iron oxide particles. The particles were found to show antioxidant

S. Paul; J. P. Saikia; S. K. Samdarshi; B. K. Konwar

2009-01-01

77

Investigation of antioxidant property of iron oxide particlesby 1?-1?diphenylpicryl-hydrazyle (DPPH) method  

Microsoft Academic Search

We investigated the antioxidant properties of iron oxide particles. Particles of different sizes were synthesized and their antioxidant potency was investigated by using an in vitro system. The antioxidant capacity assay was done via a modified DPPH method for insoluble solid materials. The percentages of DPPH scavenging were calculated for iron oxide particles. The particles were found to show antioxidant

S. Paul; J. P. Saikia; S. K. Samdarshi; B. K. Konwar

2009-01-01

78

Antioxidant properties of cap and stipe from Coprinus comatus.  

PubMed

Coprinus comatus, also called chicken drumstick mushroom, is currently commercially available in China. Hot water and ethanolic extracts were prepared from cap and stipe of C. comatus fruit bodies and their antioxidant properties were studied. Ethanolic extract from stipe showed high antioxidant activity (80.6%) at 1 mg/mL. Reducing power of hot water extracts from cap was 1.653 at 10 mg/mL. Extracts from cap showed better scavenging ability on DPPH (57.9% at 1 mg/mL) than stipe ones. Ethanolic extracts were more effective in scavenging ability on hydroxyl radicals (57.4-61.3% at 5 mg/mL) than hot water extracts. Ethanolic extracts showed moderate scavenging ability on superoxide radicals (46.3-47.0% at 20 mg/mL). Naturally occurring antioxidant components including total phenols (3.60-20.00 mg/g), tocopherols (0.58-11.93 mg/g), flavonoids (0.19-3.52 mg/g) and polysaccharides (58.52-547.86 mg/g) were found in the extracts. Overall, extracts from cap were more effective for the antioxidant properties assayed. PMID:20335995

Li, Bo; Lu, Fei; Suo, Xiaomin; Nan, Haijuan; Li, Bin

2010-03-09

79

[Antioxidant and anticancer properties of metalloporphyrins embedded in liposomes].  

PubMed

Reactive oxygen species (ROS) are implicated in many disease such as inflammation, arteriosclerosis, cancer. Therefore, a water-soluble cationic metalloporphyrins with SOD activity are studied widely as antioxidant drugs. Further, liposomes are applied to drug delivery system (DDS) as drug carriers and investigated for example disposition and stability. We designed PEG modified liposomes for avoiding reticuloendothelial system (RES) and embedded cationic metalloporphyrins for DDS, evaluated antioxidant and anticancer property. Preservation of these particle size measured DLS in an in vitro system, in order to simulate in vivo conditions of flow. Result of this measurement, we found Pluronic F-68/ liposomes have a long circulation property, and avoid fusion with plasma protein. SOD activity was determined by the stopped-flow analysis and cytochrome c assay, which allowed the evaluation of k(cat) and IC(50) for the reaction with a superoxide anion radical (.O(2)(-)). Anti cancer property was measured by cell viability test. We found that F-68/ liposomes were the most effective catalyst as antioxidant and anticancer. These results revealed that porphyrin-embedded PEG-liposomes had the property of long circulation in blood and that this compound was effective as a SOD model compound with a drug carrier capacity. PMID:17898468

Yuasa, Makoto; Oyaizu, Kenichi; Murata, Hidenori; Sahara, Yoshizumi; Hatsugai, Tomomi; Ogata, Akihiko

2007-01-01

80

Chemical and antioxidant properties of pomegranate cultivars grown in the Mediterranean region of Turkey  

Microsoft Academic Search

Arils from six pomegranate (Punica granatum L.) cultivars obtained from various sites from the Mediterranean region of Turkey were evaluated for their chemical and antioxidant properties. These properties included total phenolics (TP), total monomeric anthocyanins (TMA), soluble solids (TSS), titratable acidity (TA), individual sugars and organic acids. Antioxidant capacities of arils were determined by both the ferric reducing antioxidant power

Mustafa Ozgen; Co?kun Durgaç; Sedat Serçe; Cemal Kaya

2008-01-01

81

EVALUATION OF ANTIOXIDANT PROPERTIES OF SILYMARIN AND ITS POTENTIAL TO INHIBIT PEROXYL RADICALS IN VITRO  

Microsoft Academic Search

Silymarin (milk thistle) has been used for centuries as a natural remedy for diseases of liver and biliary tract. The present study was carried out to evaluate the in vitro free radical scavenging activity and antioxidant properties of silymarin. Antioxidant properties of silymarin were evaluated by four methods: 1) FARP assay for total antioxidant capacity; 2) DPPH radical scavenging assay;

ZARBAN ASGHAR; ZIAEE MASOOD

2008-01-01

82

Antioxidant properties of tropical and temperate herbal teas  

Microsoft Academic Search

Antioxidant properties (AOP) of thirteen tropical and five temperate herbal teas were screened. Comparisons were made with green, oolong and black teas of Camellia sinensis. The AOP studied were total phenolic content, radical-scavenging activity, ferric-reducing power and ferrous ion-chelating (FIC) ability. Tropical herbal teas were more diverse in types and more variable in AOP values than temperate herbal teas. Herbal

E. W. C. Chan; Y. Y. Lim; K. L. Chong; J. B. L. Tan; S. K. Wong

2010-01-01

83

Antioxidant properties of butein isolated from Dalbergia odorifera  

Microsoft Academic Search

The antioxidant properties of butein, isolated from Dalbergia odorifera T. Chen, were investigated in this study. Butein inhibited iron-induced lipid peroxidation in rat brain homogenate in a concentration-dependent manner with an IC50, 3.3±0.4 ?M. It was as potent as ?-tocopherol in reducing the stable free radical diphenyl-2-picrylhydrazyl (DPPH) with an IC0.200, 9.2±1.8 ?M. It also inhibited the activity of xanthine

Zhi-Jiao Cheng; Sheng-Chu Kuo; Shiuh-Chuan Chan; Feng-Nien Ko; Che-Ming Teng

1998-01-01

84

Antioxidant properties of isotorachrysone isolated from Rhamnus nakaharai  

Microsoft Academic Search

Isotorachrysone inhibited iron-induced lipid peroxidation with an IC50 value of 1.64 ± 0.08 ?M in rat brain homogenates, and was comparable in potency to butylated hydroxytoluene and was more potent than a-tocopherol or desferrioxamine. The mechanism of antioxidant properties were then examined. Isotorachrysone could scavenge the stable free radical diphenyl-p-picrylhydrazyl. And it was an efficient direct scavenger of water-soluble peroxyl

George Hsiao; Feng-Nien Ko; Chun-Nan Lin; Che-Ming Teng

1996-01-01

85

From genotype to apricot fruit quality: the antioxidant properties contribution.  

PubMed

Apricot fruit (Prunus armeniaca L.) quality strictly relates to the eating quality of fresh produce. Since consumers are more and more interested in healthy food, and apricot germplasm shows a wide choice of new selections and cultivars, apricot nutraceutical properties are under evaluation in order to select outstanding genotypes, which may link breeding and marketing. In the present study, 18 apricot genotypes of the Italian and international germplasm were evaluated over three years according to pomological, total antioxidant and total phenols attributes using principal component and hierarchical cluster analyses. The antioxidant capacity ranged from 1.24 to 11.47 ?molTE gFW?¹ and total phenols from 0.22 to 1.58 mgGAE gFW?¹. Four outstanding genotypes stood out as healthy food for fresh consumption which may also be introduced in breeding programs to produce new cultivars with improved nutraceutical values in addition to fruit qualitative attributes. PMID:23054392

Leccese, Annamaria; Bartolini, Susanna; Viti, Raffaella

2012-12-01

86

Antioxidant and antiproliferative properties of methylated metabolites of anthocyanins.  

PubMed

Anthocyanins are major flavonoids in many plant foods and have been related to health promotion. In the human organism anthocyanins are metabolised to different metabolites. One of the most important phase II reactions of flavonoids is the methylation of the catechol group. This feature is expected to have an effect on the antioxidant and antiproliferative properties of flavonoids including anthocyanins. In this work, delphinidin-3-glucoside, cyanidin-3-glucoside and petunidin-3-glucoside methylated metabolites were obtained by enzymatic hemi-synthesis. The compounds were identified as monomethylated products by HPLC-MS and NMR. The methylated metabolites were found to still retain significant radical scavenging activity and reducing activity, suggesting that they could act as potential antioxidants in vivo. The antiproliferative activity of the metabolites in comparison with the parental anthocyanins was also evaluated in three cancer cell lines by sulforhodamine B assay. The conjugation with methyl groups decreased or did not alter the antiproliferative effect of the original anthocyanin. PMID:23871042

Fernandes, Iva; Marques, Filipe; de Freitas, Victor; Mateus, Nuno

2013-05-18

87

Production, characterization and antioxidant activity of exopolysaccharides from submerged culture of Morchella crassipes.  

PubMed

The aim of this work was to investigate the fermentation optimization, molecular characterization, and antioxidant activity in vitro of exopolysaccharides (EPS) from Morchella crassipes in submerged culture. Firstly, an optimal medium for EPS production was obtained by single-factor experiment and central composite design as follows: maltose 44.79 g/L and tryptone 4.21 g/L. Then, one fraction of EPS was obtained from the culture filtrates by size exclusion chromatography and the molecular characteristics were examined by a multi-angle laser light scattering and refractive index detector system. The weight-average molar mass and the polydispersity ratio of the EPS fraction were revealed to be 1.961 × 10(4) g/mol and 1.838, respectively. FT-IR spectroscopy was used for obtaining vibrational spectra of the purified EPS fraction. Finally, the antioxidant activity of EPS was investigated and the relationship with molecular properties was discussed as well. PMID:22415552

He, Peixin; Geng, Lujing; Mao, Duobin; Xu, Chunping

2012-03-14

88

SYNTHESIS AND CHARACTERIZATION OF POLYMERIC ANTIOXIDANT DELIVERY SYSTEMS  

Microsoft Academic Search

Even though the role of oxidative stress in a variety of disease states is known, strategies to alleviate this oxidative stress by antioxidants have not been able to achieve clinical success. Particularly, treatment of oxidative stress by small molecule antioxidants has not received due attention because of the challenges associated with its delivery. Antioxidant polymers, where small molecule antioxidants are

Paritosh P. Wattamwar

2011-01-01

89

Design, synthesis and properties of novel powerful antioxidants, glutathione analogues.  

PubMed

Glutathione (GSH) is the major low-molecular weight antioxidant in mammalian cells. Thus, its analogues carrying similar and/or additional positive properties might have clinical perspectives. Here, we report the design and synthesis of a library of tetrapeptidic GSH analogues called UPF peptides. Compared to cellular GSH our designed peptidic analogues showed remarkably higher hydroxyl radical scavenging ability (EC(50) of GSH: 1,231.0 +/- 311.8 microM; EC(50) of UPF peptides: from 0.03 to 35 microM) and improved antiradical efficiency towards a stable alpha,alpha-diphenyl-beta-picrylhydrazyl (DPPH) radical. The best of UPF peptides was 370-fold effective hydroxyl radical scavengers than melatonin (EC(50): 11.4 +/- 1.0 microM). We also found that UPF peptides do not influence the viability and membrane integrity of K562 human erythroleukemia cells even at 200 microM concentration. Dimerization of GSH and UPF peptides was compared in water and in 0.9% saline solutions. The results, together with an earlier finding that UPF1 showed protective effects in global cerebral ischemia model in rats, suggest that UPF peptides might serve both as potent antioxidants as well as leads for design of powerful non-peptidic antioxidants that correct oxidative stress-driven events. PMID:17577738

Ehrlich, Kersti; Viirlaid, Säde; Mahlapuu, Riina; Saar, Külliki; Kullisaar, Tiiu; Zilmer, Mihkel; Langel, Ulo; Soomets, Ursel

2007-07-01

90

Investigation of antioxidant property of iron oxide particlesby 1?-1?diphenylpicryl-hydrazyle (DPPH) method  

NASA Astrophysics Data System (ADS)

We investigated the antioxidant properties of iron oxide particles. Particles of different sizes were synthesized and their antioxidant potency was investigated by using an in vitro system. The antioxidant capacity assay was done via a modified DPPH method for insoluble solid materials. The percentages of DPPH scavenging were calculated for iron oxide particles. The particles were found to show antioxidant potency. The DPPH scavenging increases with decrease in particle size.

Paul, S.; Saikia, J. P.; Samdarshi, S. K.; Konwar, B. K.

2009-11-01

91

Antioxidant Properties of Coffee Brews in Relation to the Roasting Degree  

Microsoft Academic Search

The antioxidant properties of coffee were studied in relation to roasting degree. In particular, the extent of the chain-breaking activity and oxygen scavenging properties of Maillard reaction products contained in coffee brews were evaluated. Samples showed very high chain-breaking and oxygen consumption activities, which did not increase linearly with increasing roasting degree. In our experimental conditions, the highest antioxidant properties

M. C. Nicoli; M. Anese; L. Manzocco; C. R. Lerici

1997-01-01

92

Antioxidant and cardioprotective properties of the sulphydryl angiotensin-converting enzyme inhibitor zofenopril.  

PubMed

Zofenopril, a new potent sulphydryl angiotensin-converting enzyme (ACE) inhibitor, is characterized by high lipophilicity, selective cardiac ACE inhibition, and antioxidant and tissue protective activities. In vitro and in vivo experiments suggest that zofenopril exerts antioxidant properties at clinically achievable tissue concentrations. In endothelial cells, zofenopril enhances nitric oxide production, attenuates atherosclerotic lesion development and inhibits adhesion molecule expression by reducing reactive oxygen species. These peculiar characteristics are reflected in the drug's cardioprotective activity, which has been shown to be greater than that of non-sulphydryl ACE inhibitors. Cardiac hypertrophy was also reduced by chronic zofenopril administration, independently of its blood pressure-reducing effect. ACE inhibitors with a sulphydryl group could have an advantage in improving vascular function and reducing cardiac impairment compared with non-sulphydryl-containing ACE inhibitors. This could explain zofenopril's remarkable clinical efficacy post-infarction, and potentially beneficial use in prevention and therapy of cardiovascular diseases, such as atherosclerosis, thrombosis and heart failure. PMID:15651714

Evangelista, S; Manzini, S

93

Antioxidant and radical scavenging properties of extracts from Ganoderma tsugae  

Microsoft Academic Search

The antioxidant activities and scavenging effects on free radicals of extracts from Ganoderma were investigated. The methanolic extracts of Ganoderma tsugae (MEGT) showed the strongest antioxidant activity of five species of Ganoderma tested. MEGT exhibited substantial antioxidant activity in the linoleic acid and rat liver microsome peroxidation systems. The antioxidant activity of MEGT was stronger than ?-tocopherol. MEGT had a

Gow-Chin Yen; Jun-Yi Wu

1999-01-01

94

Antioxidant properties of some different molecular weight chitosans.  

PubMed

Chitosan, a cationic polysaccharide, is widely employed as dietary supplement and in pharmacological and biomedical applications. Although numerous studies have focused on its applications as pharmaceutical excipients or bioactive reagents, relationships between molecular weight (Mr) and biological properties remain unclear. The focus of this study was on the antioxidant properties of several Mr chitosans. We measured the ability of seven Mr chitosans (CT1; 2.8 kDa, CT2; 17.0 kDa, CT3; 33.5 kDa, CT4; 62.6 kDa, CT5; 87.7 kDa, CT6; 604 kDa, CT7; 931 kDa) to protect plasma protein from oxidation by peroxyl radicals derived from 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH). A comparison of the antioxidant action of high Mr chitosans (CT6-CT7) with that of low Mr chitosans (CT1-CT5) showed that low Mr chitosans (CT1-CT5) were more effective in preventing the formation of carbonyl groups in plasma protein exposed to peroxyl radicals. AAPH substantially increases plasma protein carbonyl content via the oxidation of human serum albumin (HSA). We also measured the ability of these chitosans to protect HSA against oxidation by AAPH. Low Mr chitosans (CT1-CT5) were found to effectively prevent the formation of carbonyl groups in HSA, when exposed to peroxyl radicals. Low Mr chitosans were also good scavengers of N-centered radicals, but high Mr chitosans were much less effective. We also found a strong correlation between antioxidant activity and the Mr of chitosans in vitro. These activities were also determined by using the 'TPAC' test. These results suggest that low Mr chitosans (CT1-CT3) may be absorbed well from the gastrointestinal tract and inhibit neutrophil activation and oxidation of serum albumin that is frequently observed in patients plasma undergoing hemodialysis, resulting in a reduction in oxidative stress associated with uremia. PMID:19559405

Tomida, Hisao; Fujii, Takeshi; Furutani, Nobuko; Michihara, Akihiro; Yasufuku, Taira; Akasaki, Kenji; Maruyama, Toru; Otagiri, Masaki; Gebicki, Janusz M; Anraku, Makoto

2009-05-13

95

Preparation, physicochemical characterization, and antioxidant effects of quercetin nanoparticles.  

PubMed

The purpose of this study was to develop quercetin-loaded nanoparticles (QUEN) by a nanoprecipitation technique with Eudragit E (EE) and polyvinyl alcohol (PVA) as carriers, and to evaluate the antioxidant effects of quercetin (QU) and of its nanoparticles. The novel QUEN systems were characterized by particle size and morphology, yield and encapsulation efficiency, differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), (1)H nuclear magnetic resonance ((1)H NMR), and dissolution study. It was observed that the weight ratio of QU:EE:PVA at 1:10:10 carried a particle size of <85 nm, a particle distribution with polydispersity index <0.3, and its yield and encapsulation efficiency were over 99%. The results from XRD and DSC of the QUEN showed that the crystal of the drug might be converted to an amorphous state. The FT-IR and (1)H NMR demonstrated that QU formed intermolecular hydrogen bonding with carriers. The release of the drug from the QUEN was 74-fold higher compared with the pure drug. In addition, the antioxidant activity of the QUEN was more effective than pure QU on DPPH scavenging, anti-superoxide formation, superoxide anion scavenging, and anti-lipid peroxidation. PMID:17689897

Wu, Tzu-Hui; Yen, Feng-Lin; Lin, Liang-Tzung; Tsai, Tong-Rong; Lin, Chun-Ching; Cham, Thau-Ming

2007-06-28

96

Pain Behavior and Response Properties of Spinal Dorsal Horn Neurons Following Experimental Diabetic Neuropathy in the Rat: Modulation by Nitecapone, a COMT Inhibitor with Antioxidant Properties  

Microsoft Academic Search

We attempted to characterize a spinal neuronal correlate of painful neuropathy induced by diabetes mellitus (DM). Pain behavior and response properties of spinal dorsal horn neurons were determined in rats with a streptozocin-induced DM. A catechol-O-methyltransferase inhibitor with potent antioxidant properties, nitecapone, was used in an attempt to attenuate neuropathic symptoms. Behaviorally DM induced mechanical hypersensitivity that was markedly attenuated

Antti Pertovaara; Hong Wei; Jaakko Kalmari; Minna Ruotsalainen

2001-01-01

97

Emulsifying Property and Antioxidative Activity of Cuttlefish Skin Gelatin Modified with Oxidized Linoleic Acid and Oxidized Tannic Acid  

Microsoft Academic Search

Cuttlefish skin gelatins modified with oxidized linoleic acid (OLA) and oxidized tannic acid (OTA) were characterized and\\u000a determined for emulsifying properties and antioxidative activity. Modification of gelatin with 5% OTA increased the total\\u000a phenolic content and 1,1-diphenyl-2-picrylhydrazyl, 2,2?-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging\\u000a activity and ferric reducing antioxidant power of gelatin–OTA. Incorporation of OLA into gelatin (OLA-to-free amino group\\u000a molar ratio of

Tanong Aewsiri; Soottawat Benjakul; Wonnop Visessanguan; Peter A. Wierenga; Harry Gruppen

98

Nutritive value, antioxidant and antimicrobial properties of Struchium sparganophora leaves.  

PubMed

Struchium sparganophora leaf is a green leafy vegetable popularly used in soup preparations in processed and unprocessed forms in Nigeria. Fresh leaves of S. sparganophora were soaked overnight to remove its bitterness, before determination of the nutrient and antinutrient content, while sun-dried S. sparganophora leaves were extracted with rectified spirit. The antioxidant and antimicrobial properties of the extracts were subsequently determined. The result of the study revealed that the fresh leaf has high nutrient (protein, fat, crude fiber, and mineral) but low antinutrient (tannin and phytate) content. However, processing of the S. sparganophora leaf caused a significant decrease (P < .05) in both the nutrient and antinutrient contents. The ethanolic extract of the leaf contains alkaloids, tannin, saponins, phlobatannin, anthraquinone, and glycosides; furthermore, the extract has high antioxidant activity as typified by its high total phenol (5.4 g/100 g) content, reducing power (2.50 OD(700)), and free radical scavenging ability (65.2%). In addition, the extract (1.0 mg/mL) inhibited the growth of some pathogenic bacteria [Shigella dysenteriae (zone of inhibition 17.0 mm), Staphylococcus aureus (11.0 mm)] and fungi [Candida albicans (18.0 mm), Penicillium sp. (14.0 mm), Saccharomyces cerevisiae (15.0 mm)]. The inhibition of the bacterial and fungal growth was dose dependent. However, the same concentration of the extract did not inhibit the growth of Escherichia coli, Proteus sp., Pseudomonas aeruginosa, and Klebsiella sp., as well as the growth of the following fungi: Aspergillus fumigatus, Fusarium solani, and Aspergillus flavus. It could therefore be inferred that the leaf of S. sparganophora is promising both nutritionally and medicinally because of its high nutrient content, low antinutrient content, and arrays of pharmaceutically active phytochemicals with high levels of antioxidant and antimicrobial activity. PMID:16822216

Oboh, Ganiyu

2006-01-01

99

ANTIOXIDANT PROPERTIES OF HIBISCUS: SPECIES VARIATION, ALTITUDINAL CHANGE, COASTAL INFLUENCE AND FLORAL COLOUR CHANGE  

Microsoft Academic Search

WONG SK, LIM YY & CHAN EWC. 2009. Antioxidant properties of Hibiscus: species variation, altitudinal change, coastal influence and floral colour change. Antioxidant properties (AOP) of leaves and flowers of six Hibiscus species were screened. For leaves of Hibiscus rosa-sinensis and Hibiscus tiliaceus, the effects of altitudinal change and coastal influence respectively were assessed. Flowers of Hibiscus mutabilis, during colour

SK Wong; YY Lim; EWC Chan

2009-01-01

100

Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado  

Microsoft Academic Search

A total of 38 commonly consumed vegetable samples, including 3 kale, 1 rhubarb, 3 spinach, 3 broccoli, 2 green bean, 5 carrot, 10 tomato, and 11 potato samples, were investigated for their total phenolic contents (TPC) and antioxidant properties. The measured antioxidant properties included free radical scavenging activities against DPPH, superoxide anion radical (O2?), ABTS+ and peroxyl radical, and Fe2+

Kequan Zhou; Liangli Yu

2006-01-01

101

Synthesis and characterization of poly(antioxidant ?-amino esters) for controlled release of polyphenolic antioxidants.  

PubMed

Attenuation of cellular oxidative stress, which plays a central role in biomaterial-induced inflammation, provides an exciting opportunity to control the host tissue response to biomaterials. In the case of biodegradable polymers, biomaterial-induced inflammation is often a result of local accumulation of polymer degradation products, hence there is a need for new biomaterials that can inhibit this response. Antioxidant polymers, which have antioxidants incorporated into the polymer backbone, are a class of biomaterials that, upon degradation, release active antioxidants, which can scavenge free radicals and attenuate oxidative stress, resulting in improved material biocompatibility. In this work, we have synthesized poly(antioxidant ?-amino ester) (PA?AE) biodegradable hydrogels of two polyphenolic antioxidants, quercetin and curcumin. The degradation characteristics of PA?AE hydrogels and the antioxidant activity of PA?AE degradation products were studied. Treatment of endothelial cells with PA?AE degradation products protected cells from hydrogen-peroxide-induced oxidative stress. PMID:22426289

Wattamwar, Paritosh P; Biswal, Dipti; Cochran, David B; Lyvers, Adam C; Eitel, Richard E; Anderson, Kimberly W; Hilt, J Zach; Dziubla, Thomas D

2012-03-16

102

Chemical composition, antioxidant properties and anti-cholinesterase activity of Cordia gilletii (Boraginaceae) leaves essential oil.  

PubMed

This study aimed to investigate for the first time the chemical composition, the antioxidant properties and the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity of the essential oil from the leaves of Cordia gilletii De Wild (Boraginaceae). The essential oil, characterized by 23 constituents (90.1% of the total oil), was constituted by terpene derivatives (25.6%) and non-terpene derivatives (64.5%), among which aldehydes, fatty acids and alkanes were present with the percentage of 16.5%, 18.8% and 23.1%, respectively. The antioxidant activity of C. gilletii essential oil was screened by two in vitro tests: DPPH and beta-carotene bleaching test. The essential oil revealed antioxidant activity with an IC50 value of 75.0 and 129.9 microg/mL on DPPH radical and beta-carotene decoloration tests, respectively. Moreover, C. gilletii inhibited AChE enzyme with an IC50 value of 105.6 microg/mL. PMID:21425688

Bonesi, Marco; Okusa, Philippe N; Tundis, Rosa; Loizzo, Monica R; Menichini, Federica; Stévigny, Caroline; Duez, Pierre; Menichini, Francesco

2011-02-01

103

Characterization of food antioxidants, illustrated using commercial garlic and ginger preparations  

Microsoft Academic Search

Methods for the characterization of antioxidants are presented and illustrated by their application to commercial garlic and ginger preparations, since it has been widely speculated that garlic and ginger might be beneficial to human health because they exert ‘antioxidant activity’. The sample of commercial ginger powder, tested at concentrations up to 5 mg\\/ml, inhibited the peroxidation of phospholipid liposomes, but

Okezie I. Aruoma; Jeremy P. E. Spencer; Donna Warren; Peter Jenner; John Butler; Barry Halliwell

1997-01-01

104

Hydroalcoholic extracts of Indian medicinal plants can help in amelioration from oxidative stress through antioxidant properties.  

PubMed

The in vitro study of the antioxidant properties of the hydroalcoholic extracts of various Indian medicinal plants can logically help to develop a better and safer way of amelioration from oxidative stress. As aimed, the present study has been done to estimate and thereby conclude regarding the antioxidant activities of a few Indian medicinal plants, viz., Terminalia chebula, Terminalia belerica, Emblica officinalis, Caesalpinia crista, Cajanus cajan, and Tinospora cordifolia. The extracts of the plants have been subjected to the evaluation of antioxidant properties through scavenging assays for reactive oxygen species like superoxide, nitric oxide, peroxynitrite, hypochlorous acid, singlet oxygen, etc. and measurement of TEAC values and other phytochemical parameters. The phenolic and flavonoid contents of each plant have been found to be correlated to their individual antioxidant activity. The results showed the hydroalcoholic extracts of the plants were efficient indicators of their antioxidant capacity thus concreting their basis to be used as natural antioxidant. PMID:22624183

Sarkar, Rhitajit; Mandal, Nripendranath

2012-01-01

105

Antioxidative and antiviral properties of flowering cherry fruits (Prunus serrulata L. var. spontanea).  

PubMed

The phenolic compounds of many fruits have been known to be efficient cellular protective antioxidants. In this study, antioxidative and antiviral properties of flowering cherry cultivars (Prunus yedoensis, Prunus sargentii, Prunus lannesiana, and Prunus cerasus) in Korea were investigated. The antioxidant property was assayed for specific activities including 2,2-diphenyl-1-picrylhydrazyl (DPPH) hydroxy radical scavenging activity, reducing power capacity, and superoxide dismutase (SOD) like activity. In addition, antiviral activity was determined by inhibition studies on the infection cycle of porcine epidemic diarrhea virus (PEDV), measured as minimum concentration of cherry extracts that inhibited 50% of cytopathic effect (CPE) on PEDV. Our results show that the four varieties of cherries contain substantially high antioxidants and antiviral activities. In particular, P. cerasus contains higher antioxidants and antiviral activities as well as polyphenolic content than other varieties. Our data indicate that Korean native cherry cultivars could be beneficial supplements of dietary antioxidants and natural antiviral agents. PMID:20821824

Yook, Hong-Sun; Kim, Kyoung-Hee; Park, Jung-Eun; Shin, Hyun-Jin

2010-01-01

106

In vitro and in vivo antioxidant properties of ferulic acid: A comparative study with other natural oxidation inhibitors  

Microsoft Academic Search

Ferulic acid exhibits a wide range of therapeutic effects that are attributed to its potent antioxidant capacity. However, in vitro antioxidant properties of ferulic acid have not been elucidated in detail. Evidence that polyphenols, including ferulic acid, act as antioxidants in vivo is also limited. In order to elucidate in more detail the scientific background of antioxidant activities of ferulic

Shirou Itagaki; Toshimitsu Kurokawa; Chie Nakata; Yoshitaka Saito; Setsu Oikawa; Masaki Kobayashi; Takeshi Hirano; Ken Iseki

2009-01-01

107

Antioxidant properties and principal phenolic phytochemicals of Indian medicinal plants from Asclepiadoideae and Periplocoideae  

Microsoft Academic Search

The subfamily Asclepiadoideae (Apocynaceae) and the closely-related Periplocoideae are sources of many indigenous Indian medicinal plants. We surveyed antioxidant properties and total phenolic and flavonoid contents of 15 samples, representing 12 Indian medicinal plant species from these subfamilies. Total antioxidant assay was performed using the 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid and ferric-reducing antioxidant power methods. Total phenolic and flavonoid contents were measured using

Siddharthan Surveswaran; Yi-Zhong Cai; Jie Xing; Harold Corke; Mei Sun

2010-01-01

108

Standardization of antioxidant properties of honey by a combination of spectrophotometric\\/fluorimetric assays and chemometrics  

Microsoft Academic Search

The aim of this work was to establish a solid platform of analytical information for the definition\\/standardization of the antioxidant properties of honey. We investigated first the antioxidant\\/radical scavenging capacity of 14 commercial honeys of different floral and geographic origin, using a battery of spectrophotometric tests: Folin-Ciocalteu assay for phenol content (PC), ferric reducing antioxidant power (FRAP assay) for total

Giangiacomo Beretta; Paola Granata; Maria Ferrero; Marica Orioli; Roberto Maffei Facino

2005-01-01

109

Antioxidant Properties of the Edible Basidiomycete Armillaria mellea in Submerged Cultures  

PubMed Central

Antioxidant components, ascorbic acid, total flavonoids and total phenols are produced effectively by Armillaria mellea submerged cultures. Dried mycelia and mycelia-free broths obtained by A. mellea submerged cultures are extracted with methanol and hot water and investigated for antioxidant properties. Methanolic extracts from dried mycelia (MEM) and mycelia-free broth (MEB) and hot water extracts from dried mycelia (HWEM) by A. mellea submerged cultures show good antioxidant properties as evidenced by low EC50 values (<10 mg/mL). Total flavonoid is mainly found in hot water extracts; however, total phenol is rich in methanol and hot water extracts from mycelia. Ascorbic acid and total phenol contents are well correlated with the reducing power and the scavenging effect on superoxide anions. Total flavonoid content is dependent on the antioxidant activity and the chelating effect on ferrous ions. Total antioxidant component contents are closely related to the antioxidant activity and the scavenging superoxide anion ability. Results confirm that extracts with good antioxidant properties from fermenting products by A. mellea are potential good substitutes for synthetic antioxidants and can be applied to antioxidant-related functional food and pharmaceutical industries.

Lung, Ming-Yeou; Chang, Yu-Cheng

2011-01-01

110

Antioxidant Properties of the Edible Basidiomycete Armillaria mellea in Submerged Cultures.  

PubMed

Antioxidant components, ascorbic acid, total flavonoids and total phenols are produced effectively by Armillaria mellea submerged cultures. Dried mycelia and mycelia-free broths obtained by A. mellea submerged cultures are extracted with methanol and hot water and investigated for antioxidant properties. Methanolic extracts from dried mycelia (MEM) and mycelia-free broth (MEB) and hot water extracts from dried mycelia (HWEM) by A. mellea submerged cultures show good antioxidant properties as evidenced by low EC(50) values (<10 mg/mL). Total flavonoid is mainly found in hot water extracts; however, total phenol is rich in methanol and hot water extracts from mycelia. Ascorbic acid and total phenol contents are well correlated with the reducing power and the scavenging effect on superoxide anions. Total flavonoid content is dependent on the antioxidant activity and the chelating effect on ferrous ions. Total antioxidant component contents are closely related to the antioxidant activity and the scavenging superoxide anion ability. Results confirm that extracts with good antioxidant properties from fermenting products by A. mellea are potential good substitutes for synthetic antioxidants and can be applied to antioxidant-related functional food and pharmaceutical industries. PMID:22072892

Lung, Ming-Yeou; Chang, Yu-Cheng

2011-09-26

111

Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems  

Microsoft Academic Search

Microalgae have been a popular edible food, but there are no known reports on the antioxidative peptides derived from microalgae. The algae protein waste, which is normally discarded as animal feed, is a by-product during production of algae essence from microalgae, Chlorella vulgaris. Algae protein waste was hydrolyzed using pepsin, and a potent antioxidative peptide of VECYGPNRPQF was separated and

I.-Chuan Sheih; Tung-Kung Wu; Tony J. Fang

2009-01-01

112

Antioxidant properties of Mediterranean spices compared with common food additives.  

PubMed

In this study, the antioxidant properties of Mediterranean food spices (annatto, cumin, oregano, sweet and hot paprika, rosemary, and saffron) at 5% concentration and of common food additives (butylated hydroxyanisole [BHA], butylated hydroxytoluene [BHT], and propyl gallate) at 100 microg/g are compared. The ability of these compounds to inhibit lipid peroxidation was, in decreasing order, rosemary > oregano > propyl gallate > annatto > BHA > sweet paprika > cumin > hot paprika > saffron > BHT. Deoxyribose damage is partially inhibited in the presence of cumin extract that exhibits the strongest protective action. The rest of the spices also protect deoxyribose better than the BHA and BHT used in the assay. Finally, the results obtained in the assay point to the prooxidant effect of propyl gallate. Hydrogen peroxide scavenging activity is measured by using peroxidase-based assay systems. In aqueous medium, the spice extracts show lower antioxidant activity than propyl gallate, the decreasing order being cumin > oregano > annatto > rosemary > hot paprika > sweet paprika. BHA and BHT did not scavenge H2O2 Spices are able to scavenge HOCl and protect alpha1-antiproteinase. The results indicate that rosemary and oregano are more effective HOCl scavengers than the other substances analyzed, which, in decreasing order, were propyl gallate, annatto, sweet and hot paprika, saffron, and cumin. The effect of Mediterranean food spices on the oxidative stability of refined olive oil tested by the Rancimat method was compared with common food additives during storage (72 h, 2, 4, and 6 months) at room temperature. The results showed that the spice extracts analyzed have significant stabilizing effects (P < 0.05). PMID:11563520

Martínez-Tomé, M; Jiménez, A M; Ruggieri, S; Frega, N; Strabbioli, R; Murcia, M A

2001-09-01

113

The anti-oxidant properties of isothiocyanates: a review.  

PubMed

Cruciferous vegetables, such as broccoli and watercress, have been studied extensively aiming to evaluate their chemopreventive properties. Some of them have already been established using animal models. The ITCs induce Phase II enzymes related to detoxification processes of chemical carcinogens to prevent the start of carcinogenesis. They also exhibit antitumor activity at post-initiation phase, suggesting their additional role(s) in cancer prevention. Sulforaphane is the most extensively studied isothiocyanate, focused in its anti-tumoral activity and it is mainly found in great amounts in broccoli and other cruciferous. In a dose dependent manner, ITCs inhibit the cell viability of human cervical cancer cells, human pancreatic cancer cells, human hepatocellular carcinoma cells, human ovarian cancer cells, and have antiinflammatory properties in the treatment of human T-cell leukemia cells. This protective effect may be due to improved antioxidant status. Although the health effects of diet in humans are generally considered promising, there are definite challenges and limitations of the current data in better understanding of the molecular mechanisms responsible for this effect, together with the possible interactions between different dietary constituents. The survey of relevant patents on the use of isothiocyanates such as sulforaphane for cancer and cardiovascular diseases treatments is also included in this review. PMID:23978168

de Figueiredo, Sônia M; Filho, Sidney A V; Nogueira-Machado, José A; Caligiorne, Rachel B

2013-09-01

114

Antioxidative and chemopreventive properties of Vernonia amygdalina and Garcinia biflavonoid.  

PubMed

Recently, considerable attention has been focused on dietary and medicinal phytochemicals that inhibit, reverse or retard diseases caused by oxidative and inflammatory processes. Vernonia amygdalina is a perennial herb belonging to the Asteraceae family. Extracts of the plant have been used in various folk medicines as remedies against helminthic, protozoal and bacterial infections with scientific support for these claims. Phytochemicals such as saponins and alkaloids, terpenes, steroids, coumarins, flavonoids, phenolic acids, lignans, xanthones, anthraquinones, edotides and sesquiterpenes have been extracted and isolated from Vernonia amygdalina. These compounds elicit various biological effects including cancer chemoprevention. Garcinia kola (Guttiferae) seed, known as "bitter kola", plays an important role in African ethnomedicine and traditional hospitality. It is used locally to treat illnesses like colds, bronchitis, bacterial and viral infections and liver diseases. A number of useful phytochemicals have been isolated from the seed and the most prominent of them is the Garcinia bioflavonoids mixture called kolaviron. It has well-defined structure and an array of biological activities including antioxidant, antidiabetic, antigenotoxic and hepatoprotective properties. The chemopreventive properties of Vernonia amygdalina and Garcinia biflavonoids have been attributed to their abilities to scavenge free radicals, induce detoxification, inhibit stress response proteins and interfere with DNA binding activities of some transcription factors. PMID:21776245

Farombi, Ebenezer O; Owoeye, Olatunde

2011-06-23

115

Antioxidant properties of taurine in rat alveolar macrophages  

SciTech Connect

Isolated rat alveolar macrophages (RAM) which had taken-up and accumulated extracellular (0-500 {mu}M) taurine (TAU) were exposed to 0.45 {plus minus} 0.05 ppm ozone for 30 minutes in a modified tissue culture flask containing TAU-supplemented medium. Recovered cells were assayed for oxidant damage and media analyzed for leakage of intracellular components. Cell viability significantly increased, while recovery of cells decreased (possibly due to increased adherence) with increasing TAU. At 100 {mu}M (rat plasma TAU level), TAU protected against the ozone-induced increase in zymosan-stimulated chemiluminescence, diminished leakages of lipid peroxidation products and protein into the medium, and partially restored the ozone-inactivated Na{sup +}/K{sup +} ATPase activity of RAM. Efflux of oxidized glutathione was maximized and K{sup +} leakage was minimized by the addition of 250 {mu}M TAU. At 250-500 {mu}M TAU, leakages of lipid peroxidation products and protein were enhanced, while the intracellular TAU content dramatically increased. These results indicate that TAU has both direct and indirect antioxidant properties at low levels and pro-oxidant properties at high levels in RAM.

Castranova, V.; Banks, M.A.; Porter, D.W.; Martin, W.G. (National Inst. for Occupational Safety and Health, Morgantown, WV (United States) West Virginia Univ., Morgantown (United States))

1990-02-26

116

Quercetin and rutin\\/2-hydroxypropyl-?-cyclodextrin nanoparticles: obtaining, characterization and antioxidant activity  

Microsoft Academic Search

This paper presents the obtaining, characterization and antioxidant activity evaluation of quercetin\\/2- hydroxypropyl-?-cyclodextrin and rutin\\/2-hydroxypropyl-?-cyclodextrin complexes. For synthesis the crystallization from ethanol-water solution method was used, but the yields were relatively low (up to 23%). The morphology and dimensional characterization was realized by using scanning electron microscopy. The formation of complexes was revealed by differential scanning calorimetry and the antioxidant

Georgeta Coneaca; Nicoleta G. H?d?rug?c; Daniel I. H?d?rug?d

117

Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids  

Microsoft Academic Search

The antioxidant and eicosanoid enzyme inhibition properties of pomegranate (Punica granatum) fermented juice and seed oil flavonoids were studied. The pomegranate fermented juice (pfj) and cold pressed seed oil (pcpso) showed strong antioxidant activity close to that of butylated hydroxyanisole (BHA) and green tea (Thea sinensis), and significantly greater than that of red wine (Vitis vitifera). Flavonoids extracted from pcpso

Shay Yehoshua Schubert; Ephraim Philip Lansky; Ishak Neeman

1999-01-01

118

In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves  

Microsoft Academic Search

The antioxidant potency of different fractions of Moringa oleifera leaves were investigated by employing various established in vitro systems, such as ?-Carotene bleaching, reducing power, DPPH\\/superoxide\\/hydroxyl radical scavenging, ferrous ion chelation and lipid peroxidation. On the basis of in vitro antioxidant properties polyphenolic fraction of M. oleifera leaves (MOEF) was chosen as the potent fraction and used for the DNA

Arti R. Verma; M. Vijayakumar; Chandra S. Mathela; Chandana V. Rao

2009-01-01

119

Differential antioxidant properties of red wine in water soluble and lipid soluble peroxyl radical generating systems  

Microsoft Academic Search

Red wine and its components have been shown to possess cardioprotective and anti-atherogenic effects. Additionally, red wine and many of its components like catechin, epicatechin, rutin, transresveratrol and quercetin possess antioxidant properties. Oxidized low density lipoprotein (LDL) is involved in the development of an atherosclerotic lesion. Red wine, therefore, may be anti-atherogenic because of its antioxidant effects on LDL modification.

R. S. Faustino; T. A. Clark; S. Sobrattee; M. P. Czubryl; G. N. Pierce

2004-01-01

120

Quality of commercial wine vinegars evaluated on the basis of total polyphenol content and antioxidant properties  

Microsoft Academic Search

Wine vinegar derived from fruit and grain contains a certain amount of phenolic compounds which are known to possess antioxidant properties. In this paper the quality of nine samples of wine vinegar commercially available in Bangkok was evaluated according to their total polyphenol content and antioxidant capacity compared to common distilled vinegar. The total polyphenol content was expressed in gallic

Praphan Pinsirodom

121

Antioxidant Property Enhancement of Sweet Potato Flour under Simulated Gastrointestinal pH  

PubMed Central

Sweet potato is known to be rich in healthful antioxidants, but the stability of its antioxidant properties under gastrointestinal pH is very much unknown. Hence, this study aimed to evaluate the changes in antioxidant properties (total contents of phenolics and flavonoids as well as antioxidant activity) of sweet potato flour (SPF) under simulated gastrointestinal pH conditions. It was found that the yield of SPF crude phenolic extract increased from 0.29 to 3.22 g/100 g SPF upon subjection to gastrointestinal pH conditions (p < 0.05). Also elevated significantly were the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of SPF (p < 0.05). In summary, the antioxidant properties of SPF were enhanced under gastrointestinal pH conditions, suggesting that SPF might possess a considerable amount of bound phenolic and other antioxidative compounds. The antioxidant properties of SPF are largely influenced by pH and thus might be enhanced during the in vivo digestive process.

Chan, Kim Wei; Khong, Nicholas M. H.; Iqbal, Shahid; Umar, Imam Mustapha; Ismail, Maznah

2012-01-01

122

Structural characterization and antioxidant activities of polysaccharides extracted from Epimedium acuminatum.  

PubMed

The polysaccharides were extracted from Epimedium acuminatum by hot water extraction, ultrasonic-assisted extraction, enzyme extraction, and microwave-assisted extraction. The physicochemical properties of Epimedium polysaccharides were then determined by chemical composition analysis, Fourier transform infrared (FT-IR) spectroscopy, and scanning electron microscopy (SEM) analysis. Further, the antioxidant activities were studied via different methods, including DPPH assay, ABTS assay, FRAP assay and AAPH-induced erythrocyte hemolysis assay. Results showed that the physicochemical properties of different polysaccharides were similar. Antioxidant assay indicated that four polysaccharides exhibited significant antioxidant activities in a dose-dependent manner. The antioxidant activities of the polysaccharides which obtained by hot water extraction were higher than those of other polysaccharides. Overall, E. acuminatum polysaccharides might be used as potential natural antioxidant. PMID:23218266

Cheng, Haoran; Feng, Shiling; Jia, Xuejing; Li, Qianqian; Zhou, Yonghong; Ding, Chunbang

2012-10-02

123

Antioxidant properties of calcium antagonists related to membrane biophysical interactions  

Microsoft Academic Search

The antioxidant activities of representative calcium antagonists, including amlodipine, verapamil, and diltiazem, were measured in hepatic microsomal membranes by the Fe-catalyzed, hydroxyl radical–producing system (dihydroxyfumarate + Fe3+) and assessed by malondialdehyde (MDA) formation. Despite the absence of L-type calcium channels in this membrane preparation, the calcium antagonists showed dose-dependent antioxidant activity. The biophysical mechanism for calcium-antagonist antioxidant activity was evaluated

R. Preston Mason; I. Tong Mak; Mark W. Trumbore; Pamela E. Mason

1999-01-01

124

Fuel property and antioxidant effects on elastomer integrity. Phase 2. Final report, September 1985-February 1988  

SciTech Connect

The program objective was to determine if typical antioxidants used in JP-5 turbine fuel could exhibit harmful effects on fuel system elastomers as a consequence of chemical change of the antioxidants during the inhibition process. One stable and one unstable fuel (relative to peroxidation tendency), with and without an antioxidant, were stored at 43C in contact with 14 elastomer materials. Storage periods ranged from 7 to 270 days. Post-storage elastomer results indicated no deleterious effect attributable to the antioxidants. Significant changes in elastomer properties were confined to the uninhibited, unstable fuel which developed appreciable levels of peroxides over the course of the study.

Cuellar, J.P.

1988-03-01

125

Antioxidant properties of different fractions of Vitex negundo Linn  

Microsoft Academic Search

Vitex negundo Linn. (VN), belonging to family Verbenaceae, is an aromatic shrub distributed throughout India. In the ayurvedic system of medicine it is used as a drug of choice to manage pain, inflammation and other related diseases. It contains many polyphenolic compounds, terpenoids, glycosidic iridoids and alkaloids. Since polyphenolic compounds have high antioxidant potential, the antioxidant potency of V. negundo

Om Prakash Tiwari; Yamini B. Tripathi

2007-01-01

126

Gamma-irradiation improves the color and antioxidant properties of Chaga mushroom (Inonotus obliquus) extract.  

PubMed

The objective of this study was to evaluate the effect of ionizing radiation on color and antioxidative properties of Chaga mushroom (Inonotus obliquus) extract (CME). CME (10 mg/mL) was gamma-irradiated at 0, 3, 5, 7, and 10 kGy, and color, antioxidant activity, and total phenolic compound levels were then determined. The lightness and yellowness were increased (P < .05), and the redness was decreased (P < .05), as irradiation dose increased. The antioxidant parameters such as the 2-diphenyl-1-picrylhydrazyl, superoxide, and hydroxyl radical scavenging activities, ferric reducing/antioxidant power, and inhibition of lipid peroxidation increased as the irradiation dose increased. Also, the total phenolic compound levels of CME were increased (P < .05) by gamma-irradiation. These results suggest that gamma-irradiation could be considered a means for improving the antioxidant properties and the color of CME. PMID:20041791

Kim, Jae-Hun; Sung, Nak-Yun; Kwon, Sun-Kyu; Srinivasan, Periasamy; Song, Beom-Seok; Choi, Jong-Il; Yoon, Yohan; Kim, Jin Kyu; Byun, Myung-Woo; Kim, Mee-Ree; Lee, Ju-Woon

2009-12-01

127

Polyphenolics profile and antioxidant properties of Raphanus sativus L.  

PubMed

Raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological properties. Antioxidant and radical scavenging activity of R. sativus root extracted with solvents of varying polarity were evaluated using different model systems. Polyphenolic content was estimated to be in the range 13.18-63.54?mg?g?¹ dry weight, with a considerable amount being obtained with polar solvents. High-performance liquid chromatography analysis indicated the presence of an array of polyphenolics. Catechin was found to be the most abundant phenolic compound in water extract and sinapic acid, the predominant phenolic compound in methanolic, ethyl acetate and hexane extracts. The methanolic extract showed significant ferric reducing ability, moderate metal chelating activity and strong radical scavenging activity. The methanolic extract could be successfully utilised as an ingredient in functional foods. However, water extract could be more pertinent to human nutrition as it contained a significant amount of catechin, which was comparable to traditional sources like green and black tea. PMID:21714734

Beevi, Syed Sultan; Mangamoori, Lakshmi Narasu; Gowda, Bandi Boje

2011-06-30

128

Antioxidant and anti-inflammatory properties of C-phycocyanin from blue-green algae  

Microsoft Academic Search

Objective: Phycocyanin is a pigment found in blue-green algae which contains open chain tetrapyrroles with possible scavenging properties. We have studied its antioxidant properties.¶Materials and methods: Phycocyanin was evaluated as a putative antioxidant in vitro by using: a) luminol-enhanced chemiluminescence (LCL) generated by three different radical species (Oф, OH”, RO”) and by zymosan activated human polymorphonuclear leukocytes (PMNLs), b) deoxyribose

C. Romay; J. Armesto; D. Remirez; R. González; N. Ledon; I. García

1998-01-01

129

Antioxidant properties of various solvent extracts from purple basil.  

PubMed

Water, ethanol and acetone extracts from leaves and flowers of purple basil, one of the most popular spices consumed in the Thrace region of Turkey, were tested in vitro for their ability to inhibit peroxidation of lipids, to scavenge DPPH, hydrogen peroxide, superoxide anion, to reduce Fe(III) to Fe(II) and to chelate Fe(II) ions. The results showed that purple basil contained naturally occurring antioxidant components and possessed antioxidant activity which may be attributed to its lipid peroxidation inhibitory, radical scavenging and metal chelating activities. It was concluded that purple basil might be a potential source of antioxidants. PMID:22613128

Ye?ilo?lu, Ye?im; Sit, Latif?ah

2012-05-03

130

Antioxidant properties of various solvent extracts from purple basil  

NASA Astrophysics Data System (ADS)

Water, ethanol and acetone extracts from leaves and flowers of purple basil, one of the most popular spices consumed in the Thrace region of Turkey, were tested in vitro for their ability to inhibit peroxidation of lipids, to scavenge DPPH, hydrogen peroxide, superoxide anion, to reduce Fe(III) to Fe(II) and to chelate Fe(II) ions. The results showed that purple basil contained naturally occurring antioxidant components and possessed antioxidant activity which may be attributed to its lipid peroxidation inhibitory, radical scavenging and metal chelating activities. It was concluded that purple basil might be a potential source of antioxidants.

Ye?ilo?lu, Ye?im; ?it, Latif?ah

2012-09-01

131

In vitro antioxidant properties of indigenous underutilized fruits  

Microsoft Academic Search

In the present study, in vitro antioxidant and free radical scavenging capacity of methanol extracts from 10 underutilized fruits viz., Syzygium cumini, Murraya koenigii, Coccinia grandis, Opuntia dillenii, Carissa carandus, Kirganalia reticulata, Canthium parviflorum,\\u000a Lantana camara, Alangium lamarckii, and Morus alba were evaluated using established in vitro models such as ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), 2,2?azinobis(3-ethylbenzothiozoline-6-sulfonic acid) diammonium

Nataraj Loganayaki; Sellamuthu Manian

2010-01-01

132

Polyphenolic compounds and antioxidant properties in mango fruits  

Microsoft Academic Search

Fruit of eight mango genotypes were evaluated for antioxidant potential by several biochemical assays (DPPH, ABTS, ORAC, FRAP, SASR and MCC) and tested for their polyphenol composition and vitamin C contents. The significance analysis demonstrated that the antioxidant capacity of Tainong 1 fruits were significantly higher than that of other genotypes, which was about 2.1–6.3-fold higher than Guifei assayed in

Xiaowei Ma; Hongxia Wu; Liqin Liu; Quansheng Yao; Songbiao Wang; Rulin Zhan; Shanshan Xing; Yigang Zhou

2011-01-01

133

Direct and indirect antioxidant properties of inducers of cytoprotective proteins.  

PubMed

Cellular protection against oxidative and electrophile toxicities is provided by two types of small-molecule antioxidants: (i) direct antioxidants, which are redox active, short-lived, are sacrificed in the process of their antioxidant actions and need to be replenished or regenerated, and may evoke pro-oxidant effects; and (ii) indirect antioxidants, that may or may not be redox active. Indirect antioxidants activate the Keap1/Nrf2/ARE pathway resulting in transcriptional induction of a battery of cytoprotective proteins (also known as phase 2 enzymes) that act catalytically, are not consumed, have long half-lives, and are unlikely to evoke pro-oxidant effects. These protective systems are involved in a complex functional interplay, such that many cytoprotective proteins participate in the synthesis and/or regeneration of direct antioxidants, whereas some direct antioxidants are required for the catalytic functions of cytoprotective proteins. Importantly, many inducers of cytoprotective proteins have been isolated from edible plants, e. g., sulforaphane from broccoli and curcumin from turmeric. Both are pleiotropic agents with multiple biological activities that could collectively contribute to their protective effects in various animal studies, including models of carcinogenesis, hypertension, neuronal and retinal damage. In addition to inducing cytoprotective proteins, molecules like curcumin which contain Michael acceptor functionalities (olefins or acetylenes conjugated to electron withdrawing groups) and phenolic hydroxyl groups can scavenge directly and potently oxygen- and nitrogen-centered reactive intermediates. Such bifunctional antioxidants can play a dual protective role by: (i) scavenging hazardous oxidants directly and instantaneously, and (ii) inducing cytoprotective enzymes that in turn function to resolve the consequences of hazardous processes that are already in progress, and to ensure long-term protection against subsequent challenges. PMID:18327872

Dinkova-Kostova, Albena T; Talalay, Paul

2008-06-01

134

Antioxidant properties of the triphenylethylene antiestrogen drug toremifene  

Microsoft Academic Search

The aim of the present study was to investigate antioxidativity of the triphenylethylene antiestrogen toremifene. Toremifene\\u000a and its structural analogues were studied for their ability to inhibit chain reactions of lipid peroxidation and to act as\\u000a scavengers of free radicals in vitro, and the effects of toremifene were compared to those of the estrogens, tamoxifen and\\u000a known antioxidants. Moreover, the

Markku Ahotupa; Eero Mäntylä; Lauri Kangas

1997-01-01

135

Investigation of chemical compounds, antioxidant and antimicrobial properties of teucrium arduini L. (lamiaceae).  

PubMed

In this paper chemical composition of the essential oil (analysed by GC and GC-MS), the content of phenolic compounds (analysed by HPLC), quantity of total phenols and total flavonoids (analysed by UV/Vis spectrophotometer), antioxidant and antimicrobial activities of ethanolic extracts were investigated in endemic Teucrium arduini L. in population of Mt Biokovo (Croatia). The oil was characterized by a high concentration of sesquiterpene hydrocarbons (70.4%) of which ?-caryophyllene (35.2%) and germacrene D (18.7%) being the major compounds. Three phenolic compounds (quercetin, ferulic acid and rosmarinic acid) were identified and quantified in ethanolic extract of T. arduini using HPLC. The results also showed that T. arduini is a source of polyphenolic and other antioxidants with radical-scavenging and chelating properties. The ethanol extracts prepared from the leaf of T. arduini showed broad spectrum of antimicrobial activity on Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus brasiliensis, which are susceptible on concentration below or equal to 4.00 mg/mL, whilst Microsporum gypseum was resistant at investigated concentrations. PMID:23597042

Kremer, Dario; Kosir, Iztok Joze; Kosalec, Ivan; Koncic, Marijana Zovko; Potocnik, Tanja; Cerenak, Andreja; Bezic, Nada; Srecec, Sinisa; Dunkic, Valerija

2013-08-01

136

Antioxidant properties of MitoTEMPOL and its hydroxylamine.  

PubMed

Piperidine nitroxides such as TEMPOL have been widely used as antioxidants in vitro and in vivo. MitoTEMPOL is a mitochondria-targeted derivative of TEMPOL designed to protect mitochondria from the oxidative damage that they accumulate, but once there is rapidly reduced to its hydroxylamine, MitoTEMPOL-H. As little is known about the antioxidant efficacy of hydroxylamines, this study has assessed the antioxidant activity of both MitoTEMPOL and MitoTEMPOL-H. The hydroxylamine was more effective at preventing lipid-peroxidation than MitoTEMPOL and decreased oxidative damage to mitochondrial DNA caused by menadione. In contrast to MitoTEMPOL, MitoTEMPOL-H has no superoxide dismutase activity and its antioxidant actions are likely to be mediated by hydrogen atom donation. Therefore, even though MitoTEMPOL is rapidly reduced to MitoTEMPOL-H in cells, it remains an effective antioxidant. Furthermore, as TEMPOL is also reduced to a hydroxylamine in vivo, many of its antioxidant effects may also be mediated by its hydroxylamine. PMID:19058062

Trnka, Jan; Blaikie, Frances H; Logan, Angela; Smith, Robin A J; Murphy, Michael P

2009-01-01

137

Antioxidant properties of (R)-Se-aryl thiazolidine-4-carboselenoate.  

PubMed

The antioxidant potential of organoselenium compounds has been extensively investigated because oxidative stress is a hallmark of a variety of human diseases. In this study, we report the influence of substituent groups on the antioxidant activity of (R)-Se-aryl thiazolidine-4-carboselenoate (Se-PTC) in several in vitro assays. The amino group in the thiazolidine ring affects the antioxidant activity of the compound. Our data revealed that Se-PTC a had higher radical scavenging efficiency in the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(+)) assays compared to other compounds. In the ferric ion reducing antioxidant power (FRAP) assay, Se-PTC a exhibited ferric-reducing ability at concentrations as low as 5?M. However, this effect was diminished when the amino group was protected with carbamate (Se-PTC d). In the nitric oxide scavenging assay, Se-PTC c presented better NO-scavenging than Se-PTC b. However, Se-PTC a and d did not prevent NO formation at any of the tested concentrations. Se-PTC c decreased the sodium nitroprussate-induced lipid peroxidation in the cortex and hippocampus of mice. In summary, we demonstrate that Se-PTC is a promising antioxidant compound and that the compound's activity is influenced by the amino group and by the characteristics of the arylselenium substituents. Thus, these compounds may be used as synthetic antioxidants that provide protection against oxidative diseases. PMID:23830813

Victoria, Francine Novack; Martinez, Débora Martins; Castro, Micheli; Casaril, Angela M; Alves, Diego; Lenardão, Eder João; Salles, Helena Domingues; Schneider, Paulo H; Savegnago, Lucielli

2013-07-02

138

Anticancer and antioxidant properties of terpinolene in rat brain cells.  

PubMed

Abstract Terpinolene (TPO) is a natural monoterpene present in essential oils of many aromatic plant species. Although various biological activities of TPO have been demonstrated, its neurotoxicity has never been explored. In this in vitro study we investigated TPO's antiproliferative and/or cytotoxic properties using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) test, genotoxic damage potential using the single-cell gel electrophoresis (SCGE), and oxidative effects through total antioxidant capacity (TAC) and total oxidative stress (TOS) in cultured primary rat neurons and N2a neuroblastoma cells. Dose-dependent effects of TPO (at 10 mg L-1, 25 mg L-1, 50 mg L-1, 100 mg L-1, 200 mg L-1, and 400 mg L-1) were tested in both cell types. Significant (P<0.05) decrease in cell proliferation were observed in cultured primary rat neurons starting with the dose of 100 mg L-1 and in N2a neuroblastoma cells starting with 50 mg L-1. TPO was not genotoxic in either cell type. In addition, TPO treatment at 10 mg L-1, 25 mg L-1, and 50 mg L-1 increased TAC in primary rat neurons, but not in N2a cells. However, at concentrations above 50 mg L-1 it increased TOS in both cell types. Our findings clearly demonstrate that TPO is a potent antiproliferative agent for brain tumour cells and may have potential as an anticancer agent, which needs to be further studied. PMID:24084350

Aydin, Elanur; Türkez, Hasan; Ta?demir, Sener

2013-09-01

139

Antioxidant and choleretic properties of Raphanus sativus L. sprout (Kaiware Daikon) extract.  

PubMed

Brassica vegetables and glucosinolates contained therein are supposed to reduce the risk of cancer and to possess health-promoting properties. The benefits of a Brassica-based diet may be particularly expressed by eating sprouts, in which the glucosinolate content is higher than in mature vegetables. With this in mind, a first objective of this study was to evaluate the antioxidant properties of radish (Raphanus sativus L.) sprouts (Kaiware Daikon) extract (KDE), in which the glucosinolate glucoraphasatin (GRH), showing some antioxidant activity, is present at 10.5% w/w. The contribution of GRH to KDE's antioxidant activity was considered in two chemical assays (Trolox equivalent antioxidant capacity and Briggs-Rauscher methods). The total phenol assay by Folin-Ciocalteu reagent was performed to quantify the reducing capacity of KDE. Finally, on the basis of the putative choleretic properties of antioxidant plant extracts, the effect on the bile flow of KDE administration was investigated in an animal experimental model. The findings showed that KDE has antioxidant properties and significantly induced bile flow in rats administered 1.5 g/kg of body weight for 4 consecutive days. PMID:17177500

Barillari, Jessica; Cervellati, Rinaldo; Costa, Stefano; Guerra, Maria Clelia; Speroni, Ester; Utan, Aneli; Iori, Renato

2006-12-27

140

Characterization of physicochemical properties of ferulic acid  

Microsoft Academic Search

Ferulic acid (3-methoxy, 4-hydroxy cinnamic acid) is a flavoid component possessing antioxidant property. The compound is\\u000a currently under development as a new drug candidate for the treatment of the dementia. The objective of this preformulation\\u000a study was to determine the physicochemical properties of ferulic acid. Then-octanol to water partition coefficients of ferulic acid were 0.375 and 0.489 at the pHs

Young Taek Sohn; Jin Hee Oh

2003-01-01

141

Antiproliferative and antioxidant properties of anthocyanin-rich extract from açai  

Microsoft Academic Search

An anthocyanin-rich extract, generated from açai (AEA), was investigated for its antioxidant properties and antiproliferative activity against C-6 rat brain glioma cells and MDA-468 human breast cancer cells. AEA has an ORAC value of 2589?moles trolox equivalents (TE)\\/g dried powder and a DPPH radical-scavenging activity of 1208?moles TE\\/g, suggesting that AEA is an exceptional source of natural antioxidants. In addition,

Shelly Hogan; Hyun Chung; Lei Zhang; Jianrong Li; Yongwoo Lee; Yumin Dai; Kequan Zhou

2010-01-01

142

In vitro antioxidant activity of polyphenol extracts with antiviral properties from Geranium sanguineum L  

Microsoft Academic Search

Recent evidence shows that plant polyphenols exhibit antioxidant and radical scavenging properties. By three separate and complementary methods - DPPH assay, ?-carotene-linoleic acid assay and NBT-reduction assay it was established that a polyphenol-rich extract from the medicinal plant Geranium sanguineum L. with strong anti-influenza virus activity, possessed antioxidant and radical scavenging capacities. For comparative reasons caffeic acid and the synthetic

Munevver Sokmen; Maria Angelova; Ekaterina Krumova; Svetlana Pashova; Stefka Ivancheva; Atalay Sokmen; Julia Serkedjieva

2005-01-01

143

Structure–Property Relationships of Trimetazidine Derivatives and Model Compounds as Potential Antioxidants  

Microsoft Academic Search

Twenty-five compounds (trimetazidine derivatives and other compounds, mostly having a free phenolic group) were examined for their radical scavenging and antioxidant properties. Their reaction with DPPH (2,2-diphenyl-1-picrylhydrazyl) as a measure of radical scavenging capacity was assessed by two parameters, namely EC50 (the concentration of antioxidant decreasing DPPH by 50%), and log Z, a kinetic parameter proposed here and derived from

Jacek Ancerewicz; Eugenia Migliavacca; Pierre-Alain Carrupt; Bernard Testa; Françoise Brée; Roland Zini; Jean-Paul Tillement; Serge Labidalle; Daniel Guyot; Anne-Marie Chauvet-Monges; Aimé Crevat; Alain Le Ridant

1998-01-01

144

Antioxidant properties of commercial wild rice and analysis of soluble and insoluble phenolic acids  

Microsoft Academic Search

To investigate the antioxidant properties of commercial wild rice, identify and quantify soluble and insoluble phenolic acids in wild rice whole grain, the alkaline hydrolysates from crude methanol extracts (soluble fraction) and residues (insoluble fraction) were separately analysed by high performance liquid chromatography (HPLC) coupled with photodiode array detection (PAD) and quadrupole-time of flight (Q-TOF) mass spectrometry (LC–MS). The antioxidant

Yang Qiu; Qin Liu; Trust Beta

2010-01-01

145

Antioxidant Property of Emblica officinalis during Experimentally Induced Restrain Stress in Rats  

Microsoft Academic Search

The present study was aimed to investigate the antioxidant property of Emblica officinalis (E. offic- inalis) during restrain-stress in albino rat. Three groups of albino rat were employed namely control, restrain-stress (4hr\\/day for 15days) and E. officinalis +r estrain-stress. The oxidative stress was assessed by measuring the lipid peroxidation (LPO), enzymatic antioxidant status superoxide dismutase (SOD), cata- lase (CAT), and

Ramasundaram Thangaraj; Senniyanallur Rathakrishnan Ayyappan; Panneerselvam Manikandan; Jayaraman Baskaranc

2007-01-01

146

In vitro study of the antioxidative properties of the glucose derivatives against oxidation of plasma components  

Microsoft Academic Search

Oxidative stress has been implicated in the pathogenesis of variety of diseases. Since the endogenous antioxidant defense\\u000a may be not adequate to counteract the enhanced generation of oxidants, a growing interest in research for exogenous nutrients\\u000a has been observed. The present study was designed to assess in vitro the antioxidative properties of the glucose derivatives:\\u000a calcium d-glucarate, d-gluconic acid lactone,

Joanna Kolodziejczyk; Joanna Saluk-Juszczak; Barbara Wachowicz

2011-01-01

147

The in vitro and ex vivo antioxidant properties, and hypolipidemic activity of CGP 2881  

Microsoft Academic Search

This report describes the in vitro and ex vivo antioxidant properties of a new antioxidant, CGP 2881. This compound is structurally similar to probucol, in that both compounds contain bis-tertiary butyl phenyl groups. However, CGP 2881 consistently inhibited CuSO4 (Cu2+)- and macrophage (MØ)-induced oxidation of human low density lipoproteins (LDL) more potently than equimolar concentrations of probucol. CGP 2881 (1

David L Feldman; Therese C Mogelesky; Robin Sharif; W. Kit Sawyer; Michael Jeune; Chii-Whei Hu; Kenneth S Leonards; Margaret Forney Prescott

1999-01-01

148

Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea  

PubMed Central

Background In South Africa, Calpurnia aurea (Ait.) Benth is used to destroy lice and to relieve itches, to destroy maggots and to treat allergic rashes, particularly those caused by caterpillars. Antioxidants play an important role protecting against damage by reactive oxygen species. Plants containing flavonoids have been reported to possess strong antioxidant properties. Methods The antibacterial, antioxidant activities and phenolic contents of the methanol extracts of the leaves and stems of Calpurnia aurea were evaluated using in vitro standard methods. Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins. Tannins, quercetin and catechin equivalents were used for these parameters. The antioxidant activities of the stem extract of Calpurnia aurea were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Laboratory isolates of 10 bacteria species which included five Gram-positive and five Gram-negative strains were used to assay for antibacterial activity of this plant. Results The results from this study showed that the antioxidant activities of the stem extract of Calpurnia aurea as determined by the total phenol, flavonoids, and FRAP methods were higher than that of the leaves. On the other hand, the leaf extract of the plant has higher level of total flavonols and proanthocyanidins. The leaf extract also has higher radical scavenging activity as shown in 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), and 2,2¿-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. The leaf extract showed activity against seven of the bacterial organisms. Conclusion The results from this study indicate that the leaves and stem extracts of Calpurnia aurea possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as primary antioxidants. Although, the antibacterial properties of Calpurnia aurea are not as effective as the standard drugs- Chloramphenicol and Streptomycin, they still possess some activity against bacterial strains used in this study. Calpurnia aurea may therefore be a good candidate for functional foods as well as pharmaceutical plant-based products.

Adedapo, Adeolu A; Jimoh, Florence O; Koduru, Srinivas; Afolayan, Anthony J; Masika, Patrick J

2008-01-01

149

The antioxidative properties of carnosine and other drugs.  

PubMed

The antioxidative effect of various drugs on lipid peroxidation in rat serum in the presence of FeSO4 was studied. The concentration of TBA-active products decreased in the presence of carnosine and anticataract drugs (Japanese Catalin, Chinese Baineiting and Finnish Catachrom-OFTAN). Vita-iodurol (France) and Quinax (USA) were completely inert. One of feasible mechanisms underlying certain pathologies (cataract, rheumatism, etc.) is the damage of biomembranes by active oxygen species. The potent antioxidative activity of carnosine reflects the high therapeutic value of this compound. PMID:2369412

Chasovnikova, L V; Formazyuk, V E; Sergienko, V I; Boldyrev, A A; Severin, S E

1990-01-01

150

Physicochemical characterization and antioxidant activity of commercial portuguese honeys.  

PubMed

The present study evaluated the physicochemical characteristics and antioxidant activity of 13 commercial honeys from diverse floral origin, produced in Portugal. The values of electrical conductivity of cardoon and pennyroyal honeys were superior to the maximum limits defined by European legislation. Citrus, strawberry tree, and 1 sample of lavender honeys had values of diastase activity below those determined by European legislation. Strawberry tree, pennyroyal, and cardoon honeys had the highest amounts of potassium that coincided with the highest electrical conductivity. Strawberry tree honey was the most effective as antioxidant along with cardoon and heather honeys. This ability was strongly correlated with the amounts of phenols and flavonoids and not with the levels of vitamin C or proline. PMID:23957401

Aazza, Smail; Lyoussi, Badiaa; Antunes, Dulce; Miguel, Maria Graça

2013-08-01

151

Salvia leriifolia Benth (Lamiaceae) extract demonstrates in vitro antioxidant properties and cholinesterase inhibitory activity.  

PubMed

The object of the present study was to investigate the in vitro antioxidant properties and cholinesterase inhibitory activity of Salvia leriifolia Benth extracts and fractions. The functional role of herbs and spices and their constituents is a hot topic in food-related plant research. Salvia species have been used since ancient times in folk medicine for cognitive brain function and have been subjected to extensive research. Thus, we hypothesize that S leriifolia, because of its functional properties, would be a good candidate to use as a nutraceutical product for improving memory in the elderly or patients affected by Alzheimer disease (ad). To test this hypothesis, we examined the cholinesterase inhibitory activity using the modified colorimetric Ellman's method against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The n-hexane exhibited the highest activity, with inhibitory concentration 50% (IC(50)) values of 0.59 and 0.21 mg/mL, for AChE and BChE, respectively. This extract was fractionated, and 9 of these fractions (A-I) were obtained and tested. Fraction G, characterized by the presence of sesquiterpenes as major components, was the most active against AChE (IC(50) = 0.05 mg/mL). Because oxidative stress is a critical event in the pathogenesis of AD, we decided to screen the antioxidant activity (AA) using 2,2-diphenyl-1-picrylhydrazyl test, ?-carotene bleaching test, and bovine brain peroxidation (thiobarbituric acid) assay. The ethyl acetate extract showed the highest activity, with IC(50) values of 2 and 33 ?g/mL on ?-carotene bleaching test and thiobarbituric acid test, respectively. These results suggest potential health benefits of S leriifolia extracts. However, this finding requires additional investigation in vivo. PMID:21147365

Loizzo, Monica R; Tundis, Rosa; Conforti, Filomena; Menichini, Federica; Bonesi, Marco; Nadjafi, Farsad; Frega, Natale Giuseppe; Menichini, Francesco

2010-12-01

152

Basic nutritional investigation Quantitative evaluation of the antioxidant properties of garlic and shallot preparations  

Microsoft Academic Search

Objective: Shallots and garlic are an important part of the diet of many populations and there is long-held belief in their health enhancing properties. This study tested whether fresh and commer- cial shallot and garlic preparations have antioxidant properties that might justify such claims. Methods: Samples produced by pressing and extraction of bulbs were tested for their ability to decrease

Nuttakaan Leelarungrayub; Viboon Rattanapanone; Nantaya Chanarat; Janusz M. Gebicki

153

Determination of Antioxidant Properties of Aroma Extracts from Various Beans  

Microsoft Academic Search

Aroma extracts from fresh soybeans, mung beans, kidney beans, and azuki beans were prepared using simultaneous steam distillation and solvent extraction (SDE) under mild conditions (55 °C and 95 mmHg). Extracts were examined for antioxidative activities in two different assays. The aroma extracts isolated from all beans inhibited the oxidation of hexanal for nearly one month at a level of

Kwang-Geun Lee; Alyson E. Mitchell; Takayuki Shibamoto

2000-01-01

154

Free aromatic amino acids in egg yolk show antioxidant properties  

Microsoft Academic Search

Laying hens are known to be able to “bio-convert” health-promoting components from the diet into eggs. Wheat and corn are important sources of antioxidant phenolics in the human diet. Given the fact that wheat and corn are major feed ingredients of laying hens in Canada, the objectives of this study were to characterise the presence of novel phenolic compounds in

Chamila Nimalaratne; Daise Lopes-Lutz; Andreas Schieber; Jianping Wu

2011-01-01

155

Antioxidant properties of di- tert-butylhydroxylated flavonoids  

Microsoft Academic Search

Epidemiological evidence suggests an inverse relationship between dietary intake of flavonoids and cardiovascular risk. The biological activities of flavonoids are related to their antioxidative effects, but they also can be mutagenic, due to the prooxidant activity of the catechol pattern. To prevent these problems, we synthesized new flavonoids where one or two di-tert-butylhydroxyphenyl (DBHP) groups replaced catechol moiety at position

Jonathan Lebeau; Christophe Furman; Jean-Luc Bernier; Patrick Duriez; Elisabeth Teissier; Nicole Cotelle

2000-01-01

156

Investigation of the antioxidant properties of tomatoes after processing  

Microsoft Academic Search

A study was made of the antioxidant content, activity and colour of two New Zealand commercially grown tomatoes (Lycopersicon esculentum L. var. Excell and Aranca) cultivated in glasshouses using hydroponic techniques. Excell tomatoes were harvested and stored individually while cultivar Aranca was harvested by cutting the vine, which consisted of a group of eight tomatoes. Both cultivars of tomatoes were

E Sahlin; G. P Savage; C. E Lister

2004-01-01

157

Antioxidant properties and protein compositions of porcine haemoglobin hydrolysates  

Microsoft Academic Search

Porcine haemoglobin hydrolysates were prepared through hydrolysis by Alcalase followed by Flavourzyme, and their protein compositions were analyzed using Sephadex G-50 gel filtration chromatography. The antioxidant activities, including reducing power, ferrous ion chelating ability, and DPPH radical scavenging activity, of the hydrolysates were evaluated. The results showed that the hydrolysates of haemoglobin exhibited low reducing powers, but high ferrous ion

Chi-Yue Chang; Kuei-Ching Wu; Shu-Hua Chiang

2007-01-01

158

Antioxidant properties of phenolic compounds from Pelargonium reniforme.  

PubMed

Flavonoids and hydrolyzable tannins isolated from Pelargonium reniforme were evaluated for their antioxidant ability using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical generating system and a luminol-dependent chemiluminescence assay. In both assays, the polyphenols tested showed higher radical scavenging activities than the reference antioxidant, ascorbic acid (IC50 2.6-32.9 microM vs 40.9 microM in the DPPH test, and 2-25 times stronger effects in the chemiluminescence assay). A comparison of the flavonoids and the tannins showed that the latter have more potential than the former. Structural requirements for marked antioxidant activities of hydrolyzable tannins were the presence of galloyl and hexahydroxydiphenoyl groups, and apparently carbonyl (ester) functionalities in oxidatively modified dehydrohexa-hydroxydiphenoyl moieties. For flavonoids, it appeared that a catechol (3',4'-dihydroxy) element in the B-ring were important determinants and that O-glycosides were more effective than flavone-based C-glucosyls. Conspicuously, introduction of a galloyl group significantly enhanced their potentials. The demonstrated marked antioxidant effects of the polyphenols provide a clue for beneficial effects of P. reniforme in the treatment of liver disorders among several ethnic groups in areas of southern Africa. PMID:15264932

Latté, Klaus Peter; Kolodziej, Herbert

2004-07-28

159

In vitro antioxidant properties of different parts of pomegranate flowers  

Microsoft Academic Search

The present study was designed to compare the antioxidant capacity of 70% ethanol extracts of petal, stamen and receptacle of pomegranate flowers (PF) by DPPH, reducing power, hydroxyl radicals (OH), DNA degradation and DNA breaking assays, as well as the peroxide value (POV). The different parts of PF showed significant activities in all the free radical scavenging tests. Among the

Lihua Zhang; Xuemei Yang; Yuanhu Zhang; Lu Wang; Ran Zhang

2011-01-01

160

Original Contribution ANTIOXIDANT PROPERTIES OF AMANITA PHALLOIDES MUSHROOM TOXINS  

Microsoft Academic Search

SUMMARY Alpha amanitin and phalloidin are powerful hepatotoxins that belong to the main classes of toxins namely, amatoxins and phallotoxins, isolated from the deadly poisonous mushroom Amanita phalloides. It is well known that disruptions of antioxidant enzyme defense that include superoxide dismutase (SOD), catalase, glutathione peroxidase lead to generation of reactive oxygen species, followed by increase in the products of

A. Zheleva; V. Gadjeva; S. Popova

2004-01-01

161

Influence of antioxidants on the sun protection properties of hair care products.  

PubMed

Aim of this study was to determine the influence of commercially available antioxidants on sun protection properties of hair care products. To evaluate changes of human hair L*a*b-color measurements, tensile strength measurements and high pressure dynamic scanning calorimetry (HPDSC) measurements were carried out. To have a measure for the concentration of the activity of the reactive species, causing hair or color damage, chemiluminescence measurements were carried out. Before the test with the antioxidants experiments were carried out to evaluate effects of varied artificial weathering conditions on physical properties of hair. Here high relative humidity (85%) and low radiant flux (600W/m2) exhibited the biggest changes in natural hair color but the lowest changes in the in tensile strength and HPDSC measurements. All of the tested antioxidants reduced the chemiluminescence level when used in a pre-sun or after-sun formulation. According to the HPDSC measurements the antioxidants showed a slight increase of the peak temperature and therewith a hint towards a protection effect when used in a pre-sun or after-sun product. In contrast thereto some of the antioxidants reduced the tensile strength of sun care products for hair when added. A slight reduction in the lightening of natural hair color could be observed when antioxidants were present in the sun care formulations. The effect of antioxidants in sun care formulations used on dyed hair was strongly dependent on the shade of hair. The addition of some antioxidants yielded significant improvements of the protection properties of the used sun care product in some measurement methods. PMID:15645086

Meinert, Knut; Springob, Christian; Schmidt, Carl U; Kripp, Thomas C

2004-01-01

162

Antioxidant and cytoprotective properties of infusions from leaves and inflorescences of Achillea collina Becker ex Rchb.  

PubMed

Plants are the main source of molecules with antioxidant and radical scavenging properties that aid the natural defence systems of cells and may be involved in the preservation of human health, particularly preventing all the physiopathological conditions where oxidative damage is a hallmark. Achillea collina Becker ex Rchb. is a medicinal plant of the Achillea millefolium aggregate (yarrow) traditionally used, particularly in mountain areas, as an infusion or alcohol extract for its digestive, antiinflammatory, analgesic, antipyretic and wound healing properties. The aim of this study was to investigate the antioxidant capacity and cytoprotective activity against oxidative stress of infusions obtained from the leaves and inflorescences of Achillea collina Becker ex Rchb., assessed by chemical (free radical scavenging activity by DPPH and Folin Ciocalteu assay) and biological assays (in vitro model of cytotoxicity and lipid peroxidation in PC12 cells line). Infusions of leaves had the highest antioxidant properties and cytoprotective activity. The antioxidant capacity was significantly correlated with the total phenolic content but not with the cytoprotective profile. Achillea collina Becker ex Rchb. has good antioxidant and cytoprotective properties, suggesting further investigations on its chemical composition and potential health value, particularly for traditionally prepared infusions of leaves. PMID:19067389

Giorgi, Annamaria; Bombelli, Raffaella; Luini, Alessandra; Speranza, Giovanna; Cosentino, Marco; Lecchini, Sergio; Cocucci, Maurizio

2009-04-01

163

The active natural anti-oxidant properties of chamomile, milk thistle, and halophilic bacterial components in human skin in vitro.  

PubMed

The number of skin cancers continues to rise, accounting for approximately 40% of all cancers reported in the United States and approximately 9,500 deaths per year. Studies have shown reactive oxygen species (ROS) type free radicals are linked to skin cancer and aging. Therefore, it is important for us to identify agents that have anti-oxidant properties to protect skin against free radical damage. The purpose of this research is to investigate the anti-oxidant properties of bisabolol, silymarin, and ectoin that are components from chamomile, milk thistle, and halophilic bacteria, respectively. We measured the ability of bisabolol, silymarin, and ectoin to modulate the hydrogen peroxide (H2O2)-induced upregulation of ROS free radicals in normal human skin fibroblasts in vitro. Using a flow cytometry-based assay, we demonstrated that varying concentrations of these natural components were able to inhibit upregulation of H2O2-generated free radicals in human skin fibroblasts in vitro. Our results indicate components of chamomile, milk thistle, and halophilic bacteria exhibit anti-oxidant capabilities and warrant further study in clinical trials to characterize their anti-cancer and anti-aging capabilities. PMID:23884490

Mamalis, Andrew; Nguyen, Duc-Huy; Brody, Neil; Jagdeo, Jared

2013-07-01

164

Properties of bread dough with added fiber polysaccharides and phenolic antioxidants: a review.  

PubMed

During breadmaking, different ingredients are used to ensure the development of a continuous protein network that is essential for bread quality. Interests in incorporating bioactive ingredients such as dietary fiber (DF) and phenolic antioxidants into popular foods such as bread have grown rapidly, due to the increased consumer health awareness. The added bioactive ingredients may or may not promote the protein cross-links. Appropriate cross-links among wheat proteins, fiber polysaccharides, and phenolic antioxidants could be the most critical factor for bread dough enhanced with DF and phenolic antioxidants. Such cross-links may influence the structure and properties of a bread system during baking. This article presents a brief overview of our current knowledge of the fate of the key components (wheat proteins, fibers, and phenolic antioxidants) and how they might interact during bread dough development and baking. PMID:21535512

Sivam, Anusooya S; Sun-Waterhouse, Dongxiao; Quek, SiewYoung; Perera, Conrad O

2010-10-01

165

Differential antioxidant properties of red wine in water soluble and lipid soluble peroxyl radical generating systems.  

PubMed

Red wine and its components have been shown to possess cardioprotective and anti-atherogenic effects. Additionally, red wine and many of its components like catechin, epicatechin, rutin, transresveratrol and quercetin possess antioxidant properties. Oxidized low density lipoprotein (LDL) is involved in the development of an atherosclerotic lesion. Red wine, therefore, may be anti-atherogenic because of its antioxidant effects on LDL modification. This study examined the antioxidant effects of catechin, epicatechin, rutin, transresveratrol, quercetin and Merlot wines on LDL oxidation. Merlot was chosen because although other red wines have been tested, limited information exists for this variety. Oxidation was carried out with AAPH (2,2'-Azo-bis(2-amidinopropane) dihydrochloride) and AMVN (2,2'-Azo-bis(2,4-dimethylvaleronitrile)), as water and lipid soluble peroxyl radical generating systems (FRGS), respectively. This allowed us to determine the lipophilic antioxidant characteristics of the wine and its components. Conjugated diene assays were used to measure LDL oxidation over 6 hrs. In an AAPH system, all polyphenolic compounds except transresveratrol displayed an antioxidant effect. LDL oxidation by AAPH was also inhibited by aliquots of Merlot wine. No antioxidant effects were observed in an AMVN environment except for a mild antioxidant effect by quercetin. Surprisingly, incubation of LDL with Merlot wine strongly protected against oxidation by AMVN. In summary, the five phenolic compounds displayed antioxidant effects in a water soluble free radical generating system, but only quercetin showed this in a lipid soluble one. However, red wine inhibited LDL oxidation by both water and lipid soluble free radical generating systems. Our data suggest, therefore, that red wines contain unidentified antioxidants that provide protection against LDL oxidation within a lipid soluble environment. PMID:15524181

Faustino, R S; Clark, T A; Sobrattee, S; Czubryl, M P; Pierce, G N

2004-08-01

166

In vitro and in vivo antioxidant properties and DNA damage protective activity of green fruit of Ficus glomerata  

Microsoft Academic Search

This study evaluated the antioxidant properties of Ficus glomerata fruits using in vitro and in vivo assay. In order to find in vitro antioxidant properties, extract\\/fractions from F. glomerata were studied for TPC, AOA, RP, DPPH, O2-, OH scavenging activities and LPO. Among all the extract\\/fractions, FEF has shown potent antioxidant activity and was also found effective in protecting oxidative

Arti R. Verma; M. Vijayakumar; Chandana V. Rao; Chandra S. Mathela

2010-01-01

167

Anthocyanins from black sorghum and their antioxidant properties  

Microsoft Academic Search

A black, high anthocyanin sorghum variety (Tx430) grown in several environments was analyzed for anthocyanins by spectrophotometric and HPLC methods. The samples were also analyzed for antioxidant activity using the 2,2?-azinobis (3-ethyl-benzothiaziline-6-sulfonic acid) method. Two extracting solvents, 1% HCl in methanol and 70% aqueous acetone, were compared. Sorghum brans had three to four times higher anthocyanin contents than the whole

Joseph M. Awika; Lloyd W. Rooney; Ralph D. Waniska

2005-01-01

168

Methodological aspects about in vitro evaluation of antioxidant properties  

Microsoft Academic Search

Several of the most commonly used methods for in vitro determination of antioxidant capacity are reviewed in the present paper. The chemical principles of methods based either on biological oxidants (peroxyl radical, superoxide radical anion, hydrogen peroxide, hydroxyl radical, hypochlorous acid, singlet oxygen, nitric oxide radical, and peroxynitrite) or on non-biological assays (scavenging of 2,2?-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (TEAC assay), scavenging

Luís M. Magalhães; Marcela A. Segundo; Salette Reis; José L. F. C. Lima

2008-01-01

169

Antioxidant properties of myricetin and quercetin in oil and emulsions  

Microsoft Academic Search

The effect of quercetin and myricetin on the stability of sunflower oil and oil-in-water emulsions was studied by storage\\u000a experiments monitored by measurement of peroxide values, conjugated dienes, and headspace volatile analysis. Myricetin showed\\u000a strong antioxidant activity in oils stored at 60 or 30°C and in oil-in-water emulsions stored at 30°C, whether tocopherols\\u000a or citric acid were present or not;

Andrea Roedig-Penman; Michael H. Gordon

1998-01-01

170

Polyphenolic compounds and antioxidant properties of selected China wines  

Microsoft Academic Search

Thirty-seven China wines, produced from different geographical origins, were examined in this study. The antioxidant activity of wines was measured by different analytical methods: oxygen radical absorbance capacity (ORAC), reducing power, 2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH·), hydroxyl radical-scavenger activity, superoxide radical-scavenger activity, lipid peroxidation and chelating capacity. Furthermore,total phenols, total flavonoids, total flavanols and total anthocyanins of wines were determined.

Hua Li; Xiaoyu Wang; Yong Li; Peihong Li; Hua Wang

2009-01-01

171

The antioxidant properties of gallic acid and allied compounds  

Microsoft Academic Search

Summary  The differences between animal fats and common vegetable oils as regards their protection by antioxidants are briefly reviewed;\\u000a in general, the former can be stabilized by di- and poly-phenolic inhibitors and by inhibitols, the latter by acid-type inhibitors\\u000a such as tartaric, phosphoric and other acids; these acids reinforce the action of the inhibitols which occur naturally in\\u000a vegetable oils or

Calvin Golumbic; H. A. Mattill

1942-01-01

172

Antioxidant properties of ferulic acid and its related compounds.  

PubMed

Antioxidant activity of 24 ferulic acid related compounds together with 6 gallic acid related compounds was evaluated using several different physical systems as well as their radical scavenging activity. The radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) decreased in the order caffeic acid > sinapic acid > ferulic acid > ferulic acid esters > p-coumaric acid. In bulk methyl linoleate, test hydroxycinnamic acids and ferulic acid esters showed antioxidant activity in parallel with their radical scavenging activity. In an ethanol-buffer solution of linoleic acid, the activity of test compounds was not always associated with their radical scavenging activity. Ferulic acid was most effective among the tested phenolic acids. Esterification of ferulic acid resulted in increasing activity. The activity of alkyl ferulates was somewhat influenced by the chain length of alcohol moiety. When the inhibitory effects of alkyl ferulates against oxidation of liposome induced by AAPH were tested, hexyl, octyl, and 2-ethyl-1-hexyl ferulates were more active than the other alkyl ferulates. Furthermore, lauryl gallate is most effective among the tested alkyl gallates. These results indicated that not only the radical scavenging activity of antioxidants, but also their affinity with lipid substrates, might be important factors in their activity. PMID:11902973

Kikuzaki, Hiroe; Hisamoto, Masashi; Hirose, Kanae; Akiyama, Kayo; Taniguchi, Hisaji

2002-03-27

173

Essential Oils Composition and Antioxidant Properties of Three Thymus Species  

PubMed Central

The essential oils of three wild-growing Thymus species, collected from west of Iran during the flowering stage, were obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS). Under the optimum extraction and analysis conditions, 44, 38, and 38 constituents (mainly monoterpenes compounds) were identified in T. kotschyanus Boiss. and Hohen, T. eriocalyx (Ronniger) Jalas, and T. daenensis subsp lancifolius (Celak) Jalas which represented 89.9%, 99.7%, and 95.8% of the oils, respectively. The main constituents were thymol (16.4–42.6%), carvacrol (7.6–52.3%), and ?-terpinene (3–11.4%). Antioxidant activity was employed by two complementary test systems, namely, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging and ?-carotene/linoleic acid systems. Antioxidant activity of polar subfraction of T. daenensis subsp lancifolius (Celak) Jalas was found to be higher than those of the others in DPPH assay, while nonpolar subfraction of T. eriocalyx (Ronniger) Jalas has most antioxidant activity in ?-carotene/linoleic acid test (19.1 ± 0.1??g/mL and 96.1 ± 0.8% inhibition rate, resp.).

Amiri, Hamzeh

2012-01-01

174

Antioxidant properties of the wild edible mushroom Lactarius salmonicolor.  

PubMed

The fruiting bodies of the edible mushroom Lactarius salmonicolor (Russulaceae) collected from the wild were extracted with solvents of increasing polarity and evaluated for their antioxidant capacity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Additionally, their total phenolic content was evaluated by a modification of the Folin-Ciocalteu method. The methanol extract showed the most potent radical scavenging activity (inhibition of 36.7% of DPPH at 3 mg/mL and 6.8 mg gallic acid equivalents/g of dry extract), and was further fractionated. Total phenolics and antioxidant activity found to be significant higher in the fractions of increased polarity. The phytochemical investigation which was performed by chromatographic and spectrometric techniques led to the isolation of nine compounds from the methanol extract and the identification of 10 fatty acids and fatty acids esters from the dichloromethane extract. Evaluation of the antioxidant activity of isolated compounds showed p-hydroxybenzoic acid (4) to be the most active. Among the various compounds found, the rare azulene type 7-acetyl-4-methylazulene-1-carboxylic acid (1) has been identified, which had been isolated only once before from L. deliciosus. Therefore, this compound seems to have some chemotaxonomic value for Lactrarius species exuding orange to red milk. PMID:23957359

Athanasakis, Georgios; Aligiannis, Nektarios; Gonou-Zagou, Zacharoula; Skaltsounis, Alexios-Leandros; Fokialakis, Nikolas

2013-08-01

175

Radiation hydrolysate of tuna cooking juice with enhanced antioxidant properties  

NASA Astrophysics Data System (ADS)

Tuna protein hydrolysates are of increasing interest because of their potential application as a source of bioactive peptides. Large amounts of tuna cooking juice with proteins and extracts are produced during the process of tuna canning, and these cooking juice wastes cause environmental problems. Therefore, in this study, cooking juice proteins were hydrolyzed by irradiation for their utilization as functional additives. The degree of hydrolysis of tuna cooking juice protein increased from 0% to 15.1% at the absorbed doses of 50 kGy. To investigate the antioxidant activity of the hydrolysate, it was performed the ferric reducing antioxidant power (FRAP) assay, and the lipid peroxidation inhibitory and superoxide radical scavenging activities were measured. The FRAP values increased from 1470 ?M to 1930 ?M and IC50 on superoxide anion was decreased from 3.91 ?g/mL to 1.29 ?g/mL at 50 kGy. All of the antioxidant activities were increased in the hydrolysate, suggesting that radiation hydrolysis, which is a simple process that does not require an additive catalysts or an inactivation step, is a promising method for food and environmental industries.

Choi, Jong-il; Sung, Nak-Yun; Lee, Ju-Woon

2012-08-01

176

Analysis method and characterization of the antioxidant capacity of vitamin E-interactive polysulfone hemodialyzers  

Microsoft Academic Search

The lipophilic antioxidant vitamin E was used as a surface modifier (or coating agent) of hollow-fiber hemodialyzer membranes with the aim of increasing their biocompatibility and preventing oxidative stress, which are the main clinical drawbacks in hemodialysis (HD) therapy. At present, the redox chemistry of vitamin E-modified dialyzers is not well characterized and there is no standard method to assess

Alessandro Floridi; Marta Piroddi; Francesca Pilolli; Yutaka Matsumoto; Masaharu Aritomi; Francesco Galli

2009-01-01

177

Simulated Gastrointestinal pH Condition Improves Antioxidant Properties of Wheat and Rice Flours  

PubMed Central

The present study was conducted to evaluate the antioxidant properties of wheat and rice flours under simulated gastrointestinal pH condition. After subjecting the wheat and rice flour slurries to simulated gastrointestinal pH condition, both slurries were centrifuged to obtain the crude phenolic extracts for further analyses. Extraction yield, total contents of phenolic and flavonoids were determined as such (untreated) and under simulated gastrointestinal pH condition (treated). 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging activity, 2,2?-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical cation (ABTS•+) scavenging activity, ferric reducing antioxidant power (FRAP), beta-carotene bleaching (BCB) and iron chelating activity assays were employed for the determination of antioxidant activity of the tested samples. In almost all of the assays performed, significant improvements in antioxidant properties (p < 0.05) were observed in both flours after treatment, suggesting that wheat and rice flours contain considerably heavy amounts of bound phenolics, and that their antioxidant properties might be improved under gastrointestinal digestive conditions.

Chan, Kim Wei; Khong, Nicholas M. H.; Iqbal, Shahid; Ismail, Maznah

2012-01-01

178

Color, betalain pattern, and antioxidant properties of cactus pear (Opuntia spp.) clones.  

PubMed

Total phenolics, ascorbic acid, and betalain contents of differently colored cactus pear clones (nine Opuntia ficus-indica [L.] Mill. clones and one O. robusta Wendl. clone) were investigated and related to their respective antioxidant potential assessed by Trolox-equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays. TEAC and ORAC values were very highly correlated with each other and also with values for total phenolics, betalain contents, and ascorbic acid concentrations. Total phenolics had the greatest contribution to ORAC and TEAC values. High-performance liquid chromatography (HPLC)-diode array detector (DAD)-tandem mass spectrometry (MS/MS) measurements of cactus pear juices permitted the differentiation of the clones based on variations in pigment patterns and betalain concentrations. The red and yellow betalains were absent in lime green colored cactus fruits. The ratio and concentration of these pigments were responsible for the yellow, orange, red, and purple colors in the other clones. Progeny of purple and lime green colored parents were characterized by 12% and 88% of plants bearing lime green and purple fruit, respectively. This implies that the genes for betalain production were lacking in the lime green fruits but could be provided by a parent with a complete set of genes, that is, purple fruits. Besides known pigments typical of Cactaceae, two unexpected betalains were identified. Whereas gomphrenin I was found for the first time in tissues of cactus plants, methionine-betaxanthin has never been described before as a genuine betalain. In addition to their alleged health-promoting properties, various combinations of yellow betaxanthins and red-purple betacyanins may allow the development of new food products without using artificial colorants. PMID:15656686

Stintzing, Florian C; Herbach, Kirsten M; Mosshammer, Markus R; Carle, Reinhold; Yi, Weiguang; Sellappan, Subramani; Akoh, Casimir C; Bunch, Ron; Felker, Peter

2005-01-26

179

Isolation, characterization and antioxidative activity of C-phycocyanin from Limnothrix sp. strain 37-2-1.  

PubMed

C-phycocyanin (C-PC) is a blue colored accessory photosynthetic pigment found in cyanobacteria. Some of the medicinal properties of Spirulina have been attributed to this pigment, which includes anticancer, antioxidant, and anti-inflammatory activity. We have screened cyanobacteria isolated from freshwater habitats in Florida for their high content of C-PC. Of 125 strains tested, one filamentous strain identified as Limnothrix sp. was selected for further research. This strain produced 18% C-PC of total dry biomass. Here we describe a simple method for obtaining C-PC of high purity without the use of ion exchange chromatography. The procedure is based on pigment precipitation from the cell lysate with an appropriate concentration of ammonium sulfate, then purification with activated carbon and chitosan, followed by a sample concentration using tangential flow filtration. We have shown that when the lower concentration of ammonium sulfate was used, C-PC with higher purity index was recovered. Characterization of C-PC from Limnothrix showed that it had an absorbance maximum at 620nm and fluorescence at 639nm. The molecular mass of intact C-PC was estimated to be ~50kDa with ? and ? subunits forming dimmers. When C-PC content per unit biomass was compared to that of marketed Spirulina powder, we found that Limnothrix was superior. C-phycocyanin from Limnothrix had an antioxidative activity on DPPH free radicals similar to that found in a natural antioxidant - rutin. PMID:22353597

Gantar, Miroslav; Simovi?, Dragan; Djilas, Sonja; Gonzalez, Walter W; Miksovska, Jaroslava

2012-02-14

180

Synthesis, characterization and antioxidant activity copper-quercetin complex  

NASA Astrophysics Data System (ADS)

Quercetin (3,3',4',5,7-pentahydroxyflavone) one of the most abundant dietary flavonoids, has been investigated in the presence of Cu(II) in methanol. The spectroscopic studies (UV-vis, 1H NMR and IR) were useful to assess the relevant interaction of Quercetin with Cu(II) ions, the chelation sites and dependence of the complex structure from the metal/ligand ratio. A 1:2 (L:M) complex was indicated by Job's method of continuous variation, which was applied to ascertain the stoichiometric composition of the complex. The antioxidant activities of the compounds were evaluated by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The complexed flavonoid was much more effective free radical scavengers than the free flavonoids.

Bukhari, S. Birjees; Memon, Shahabuddin; Mahroof-Tahir, M.; Bhanger, M. I.

2009-01-01

181

Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities.  

PubMed

Thirty-four strains of lactic acid bacteria (seven Bifidobacterium, 11 Lactobacillus, six Lactococcus, and 10 Streptococcus thermophilus) were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation (TAA(AA) and TAA(LA)), trolox-equivalent antioxidant capacity (TEAC), intracellular glutathione (TGSH), and superoxide dismutase (SOD). Wide dispersion of each of TAA(AA), TAA(LA), TEAC, TGSH, and SOD occurred within bacterial groups, indicating that antioxidative properties are strain specific. The strains Bifidobacterium animalis subsp. lactis DSMZ 23032, Lactobacillus acidophilus DSMZ 23033, and Lactobacillus brevis DSMZ 23034 exhibited among the highest TAA(AA), TAA(LA), TEAC, and TGSH values within the lactobacilli and bifidobacteria. These strains were used to prepare a potentially antioxidative probiotic formulation, which was administered to rats at the dose of 10(7), 10(8), and 10(9) cfu/day for 18 days. The probiotic strains colonized the colon of the rats during the trial and promoted intestinal saccharolytic metabolism. The analysis of plasma antioxidant activity, reactive oxygen molecules level, and glutathione concentration, revealed that, when administered at doses of at least 10(8) cfu/day, the antioxidant mixture effectively reduced doxorubicin-induced oxidative stress. Probiotic strains which are capable to limit excessive amounts of reactive radicals in vivo may contribute to prevent and control several diseases associated with oxidative stress. PMID:22790540

Amaretti, Alberto; di Nunzio, Mattia; Pompei, Anna; Raimondi, Stefano; Rossi, Maddalena; Bordoni, Alessandra

2012-07-12

182

Characterization and Properties of Nanoparticles  

NASA Astrophysics Data System (ADS)

Nanoparticles and other nanostructured materials are increasingly subject to a wide variety of studies. Although it is often ignored, in many circumstances the character and properties of nano-structured materials vary as a function of time, are altered by coatings and are environmentally dependent. This talk will outline some of the general issues related to characterization of nanoparticles and show measurements of the time and environmentally dependent behaviors of iron metal-core oxide-shell and ceria nanoparticles. Iron nanoparticles are of interest for several reasons including their potential use for medical applications and reduction of environmental contaminants such as chlorinated hydrocarbons or chromates. A study of the changes that occur when iron nanoparticles are exposed to water and the impact of the changes on particle reactivity and reaction pathway will be discussed. For this work, XPS, TEM, BET and XRD have been used to obtain information about the changes that take place in the particles as a function of time. Because of an ability to store and release oxygen, ceria is important for catalytic, fuel cell and possibly health applications. Other researchers have shown that as CeO2 particles decrease in size, the Ce valence state changes from a Ce^+4 to a Ce^+3. We have found that this transformation is environmentally dependent. Five nm particles can change from +4 to +3 and back to +4, as the environment is altered from oxidizing to reducing conditions and back. These changes can be observed in solution using optical adsorption and may have impacted measurements intended to examine band-gap changes due to particle size. XPS measurements of particles fully corroborate the real time solution measurements. These time and environmentally dependent measurements create important sample and characterization handling challenges.

Baer, Donald

2008-05-01

183

Investigations on the binding and antioxidant properties of the plant flavonoid fisetin in model biomembranes  

Microsoft Academic Search

Plant flavonoids are emerging as potent therapeutic drugs for free radical mediated diseases, for which cell membranes generally serve as targets for lipid peroxidation and related deleterious effects. Screening and characterization of these ubiquitous, therapeutically potent polyphenolic compounds, require a clear understanding regarding their incorporation and possible location in membranes, as well as quantitative estimates of their antioxidative and radical

Bidisa Sengupta; Anwesha Banerjee; Pradeep K Sengupta

2004-01-01

184

Phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata Willd. subsp. mucronata Willd  

PubMed Central

Background Several plants traditionally used in treatment of a variety of infections in South Africa are reported in ethnobotanical surveys. Many of these plants including Ziziphus mucronata subsp. mucronata lack scientific reports to support their medicinal importance. Methods The antioxidant activities and phenolic contents of the acetone, ethanol and aqueous extracts of the stems of Z. mucronata subsp. mucronata were evaluated using in vitro standard methods. The total phenol, total flavonoids and proanthocyanidin content were determined spectrophotometrically. Quercetin, Tannic acid and catechin equivalents were used for these parameters. The antioxidant activities of the stem bark extracts of this plant were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Results The quantity of the phenolic compounds, flavonoids and proanthocyanidins detected differ significantly in the various extracts. The phenolics were significantly higher than the flavonoids and proanthocyanidin contents in all the extracts investigated. The ferric reducing ability and the radical scavenging activities of the extracts were very high and dose-dependent. The ethanol extract had the highest antioxidant activity, followed by the acetone extract while the aqueous extract was the least active. Reacting with ABTS, the 50% inhibitory concentrations (IC50) were (0.0429 ± 0.04 mg/ml) for aqueous, (0.0317 ± 0.04 mg/ml) for acetone and (0.0306 ± 0.04 mg/ml) for ethanol extracts while they inhibited DPPH radical with 50% inhibitory concentration (IC50) values of 0.0646 ± 0.02 mg/ml (aqueous), 0.0482 ± 0.02 mg/ml (acetone) and 0.0422 ± 0.03 mg/ml (ethanol). Conclusions A correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant. This study, therefore, demonstrated that Z. mucronata subsp. mucronata has strong antioxidant property and free radical scavenging capability.

2011-01-01

185

[Influence of the composition of essential oils on their antioxidant and antiradical properties].  

PubMed

The antiradical and antioxidant properties of essential oil mixtures (EOMs) with various compositions were studied and compared in model systems of hexanal autooxidation, thermal oxidation of methyl linoleate and beta3-carotene, and in a reaction with the stable diphenylpicrylhydrazyl radical. It was found that all EOMs under investigation exhibited antiradical activity. The highest antiradical activity was observed for an EOM containing monoterpene hydrocarbons as the main components (the phenol content was low). The antioxidant activity of all EOMs was from 60 to 98% and depended on the model system composition and the method of assay. The mixture with the highest phenol content exhibits the highest antioxidant activity level in the hexanal autooxidation system. EOMs with a high content of phenols and alpha- and y-terpinenes were the most efficient antioxidants in the beta3-carotene model system. The study confirmed the possibility to vary the antioxidant and antiradical properties of essential oils by preparing their mixtures with a specific composition. PMID:22567894

Misharina, T A; Alinkina, E S; Fatkullina, L D; Vorob'eva, A K; Medvedeva, I B; Burlakova, E B

186

Antioxidant properties of chitosan coatings on frozen Atlantic salmon fillet portions  

Microsoft Academic Search

Atlantic salmon contain omega-3 fatty acids, which play important roles in promoting human health but are highly susceptible to oxidation. Chitosan has been shown to have antioxidant properties which could be beneficial in extending the shelf life of Atlantic salmon; however, the effects of chitosan molecular size on oxidation of salmon fillets have not been reported. The primary objective of

M. D. Hammond; D. I. Skonberg

2011-01-01

187

Antioxidant Properties of Chitosan Coatings on Frozen Atlantic Salmon Fillet Portions  

Microsoft Academic Search

Atlantic salmon contain omega-3 fatty acids, which play important roles in promoting human health but are highly susceptible to oxidation. Chitosan has been shown to have antioxidant properties which could be beneficial in extending the shelf life of Atlantic salmon; however, the effects of chitosan molecular size on oxidation of salmon fillets have not been reported. The primary objective of

M. D. Hammond; D. I. Skonberg

2012-01-01

188

Assessment of antioxidant activity and rheological properties of wheat and buckwheat milling fractions  

Microsoft Academic Search

This study examined the antioxidant properties of the ethanolic extracts of wheat milling fractions (wheat flour type 500 and type 850, and bran) and their polyphenol and tocopherol content, and rheological characteristics of wheat dough supplemented with buckwheat flours (light and wholegrain). The results obtained in this study were correlated with our previously published data on wheat flour type 400,

Ivana Sedej; Marijana Saka?; Anamarija Mandi?; Aleksandra Mišan; Vesna Tumbas; Miroslav Hadna?ev

189

Nutritive Value and Antioxidant Properties of Cereal Gruels Produced from Fermented Maize and Sorghum  

Microsoft Academic Search

Cereal gruels prepared from fermented maize (yellow and white) and sorghum seeds are the forms in which cereal are popularly consumed in tropical Africa, mostly as weaning food for children. The present study sought to determine the nutritional and antioxidant properties of cereal gruels produced from maize (yellow and white) and sorghum seeds. Seeds of maize (yellow and white) and

G. Oboh; T. V. Amusan

2009-01-01

190

Antioxidant properties of Myristica fragrans (Houtt) and its effect on selected organs of albino rats  

Microsoft Academic Search

Aqueous extract of the seed of Myristica fragrans (nutmeg) was evaluated for its phytochemical constituents, antinutrients and antioxidant properties. Toxicological investigation was also carried out using six groups of Wistar albino rats. The treatment groups were administered varying doses (100-500 mg\\/kg body weight) of the extract for a period of 28 days. The animals were sacrificed and their livers, kidneys,

M. T. Olaleye; Afolabi C. Akinmoladun; A. A. Akindahunsi

191

Anti-oxidant and immunomodulatory properties of seabuckthorn ( Hippophae rhamnoides)—an in vitro study  

Microsoft Academic Search

This study was designed to determine the anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides) using lymphocytes as a model system. Chromium(VI) as potassium dichromate was used to induce oxidative damage. The production of free radicals by chromium and the ability of alcoholic leaf and fruit extracts of seabuckthorn to inhibit the oxidative damage induced by chromium was investigated. Addition

S Geetha; M Sai Ram; V Singh; G Ilavazhagan; R. C Sawhney

2002-01-01

192

Influence of jet-cooking Prowashonupana barley flour on phenolic composition, antioxidant activities, and viscoelastic properties  

Technology Transfer Automated Retrieval System (TEKTRAN)

The influence of jet-cooking Prowashonupana barley flour on total phenolic contents, antioxidant activities, water holding capacities, and viscoelastic properties was studied. Barley flour was jet-cooked without or with pH adjustment at 7, 9, or 11. Generally, the free phenolic content and antioxi...

193

Anti-angiogenic, Antioxidant, and Anticarcinogenic Properties of a Novel Anthocyanin-Rich Berry Extract Formula  

Microsoft Academic Search

Edible berry anthocyanins possess a broad spectrum of therapeutic and anti-carcinogenic properties. Berries are rich in anthocyanins, compounds that provide pigmentation to fruits and serve as natural antioxidants. Anthocyanins repair and protect genomic DNA integrity. Earlier studies have shown that berry anthocyanins are beneficial in reducing age-associated oxidative stress, as well as in improving neuronal and cognitive brain function. Six

D. Bagchi; C. K. Sen; M. Bagchi; M. Atalay

2004-01-01

194

Inhibitory effect of raspberries on starch digestive enzyme and their antioxidant properties and phenolic composition  

Microsoft Academic Search

Seven primocane fall-bearing raspberry (Rubus idaeus L.) cultivars, Nova (red), Dinkum (red), Heritage (red), Autumn Britten (red), Josephine, Anne (yellow), Fall Gold (yellow) were analysed for potential health promoting properties including their inhibitory effect on starch and fat digestive enzymes, antioxidant activities, and phenolic composition. The tested raspberry extracts showed no detectable inhibition of pancreatic ?-amylase and lipase. However, all

Lei Zhang; Jianrong Li; Shelly Hogan; Hyun Chung; Gregory E. Welbaum; Kequan Zhou

2010-01-01

195

Antioxidant property of selected Ocimum species and their secondary metabolite content  

Microsoft Academic Search

In this study, chemical composition and antioxidative properties of eight selected Ocimum species (Ocimum gratissimum, Ocimum americanum, Ocimum minimum, Ocimum citriodorum, Ocimum kilimandscharicum, Ocimum grandiflorum, Ocimum lamiifolium, and Ocimum selloi) was investigated. Leaves of these plants were extracted using methanol. The quantitative analysis of phenolic constituents was determined using high-performance liquid chromatography. Total phenolic content was estimated using Folin-Ciocalteu reagent

F. Lukmanul Hakkim; Girija Arivazhagan; R. Boopathy

2008-01-01

196

The Antimicrobial Activity, Mosquito Larvicidal Activity, Antioxidant Property and Tyrosinase Inhibition of Piper betle  

Microsoft Academic Search

The essential oil and methanolic and aqueous extracts of Piper betle L. were assayed for their anti- microbial activity, mosquito larvicidal activity, antioxidant property and mushroom tyrosinase inhibition. The methanolic and aquaous extracts showed strong activity against the yeasts: C. albicans ,a ndM. pachydermatis. The crude essential oil exhibited a broad-spectrum strong antimicrobial activity against all test organisms. The strongest

Li-Ching Morgan Row; Jiau-Ching Ho

197

Evaluation of antioxidant and antimicrobial properties of finger millet polyphenols ( Eleusine coracana)  

Microsoft Academic Search

Phenolic acids from finger millet (Eleusine coracana) milled fractions (whole flour, seed coat, 3%, 5% and 7%) were isolated and their antioxidant and antimicrobial properties were evaluated. Acidic methanol extracts from seed coat to whole flour were rich in polyphenol content and were found to be stable up to 48h at pH 4, 7, and 9 as studied by ultraviolet

Varsha Viswanath; Asna Urooj; N. G. Malleshi

2009-01-01

198

Antitumoral and antioxidant effect of essential oils and in vitro antioxidant properties of essential oils and aqueous extracts from Salvia pisidica  

Microsoft Academic Search

The aim of the work was to investigate antitumoral effect of essential oils on cancer cells and their possible protective\\u000a (antioxidant) effects against hydrogen peroxide-induced cytotoxicity. Also, in vitro antioxidant properties of essential oils\\u000a and aqueous extracts from wild form and cultivated form of Salvia pisidica were compared.\\u000a \\u000a \\u000a We found out that essential oils from wild form (IC50 128, and

Aysun Ozkan; Ayse Erdogan; Munevver Sokmen; Saadet Tugrulay; Orhan Unal

2010-01-01

199

Novel EPR characterization of the antioxidant activity of tea leaves  

NASA Astrophysics Data System (ADS)

Electron paramagnetic resonance (EPR) spectroscopy is utilized to investigate several categories of green and black tea: Twining green tea (TGT), Chinese green tea (CGT), Red-labels black tea (RBT). Basically, two EPR signals from all the studied samples are observed: One of them is a very weak sharp EPR signal with ? Hpp?10 G and g-factor=2.00023 superimposed on the other broad signal with ? Hpp?550 G and g-factor=2.02489. The broad signal is a characteristic one of manganese(II) complex, while the sharp signal is related to a stable radical of aromatic origin exist in a powder condition. The feature of the manganese EPR signal is attributed to manganese(II) complex and reflected the molecular behavior of Mn(II) in the protein system of the natural leaves. The sharp signal, which is most probably due to a semiquinones radicals, is observed at room temperature and its intensity is remarkably affected by photo degradation of the studied samples. The intensity of manganese(II) EPR signal is found to be related to ageing and disintegration of the tea leaves. Moreover, direct relation between the relative intensity of the semiquinones radical signal and antioxidant activity of the studied samples was also correlated.

Morsy, M. A.; Khaled, M. M.

2002-04-01

200

Effect of Extraction Temperature on Functional Properties and Antioxidative Activities of Gelatin from Shark Skin  

Microsoft Academic Search

Physico-chemical properties, functional properties, and antioxidative acitivities of gelatin from the skins of brownbanded\\u000a bamboo shark (BBS; Chiloscyllium punctatum) and blacktip shark (BTS; Carcharhinus limbatus), as affected by extraction temperature, were investigated. ?-Amino acid group content and surface hydrophobicity of both\\u000a gelatins from both species increased as the extraction temperature increased (P?

Phanat Kittiphattanabawon; Soottawat Benjakul; Wonnop Visessanguan; Fereidoon Shahidi

201

Antioxidant and biochemical properties of protein hydrolysates prepared from Silver carp ( Hypophthalmichthys molitrix)  

Microsoft Academic Search

The antioxidant and biochemical properties of enzymatically hydrolyzed silver carp (Hypophthalmichthys molitrix) protein were studied. The molecular weight of the main peaks of the hydrolysates by both Alcalase and Flavourzyme was lower than 5000Da. The hydrolysates treated by Alcalase for ?1.5h (hydrolysis time) showed that the relative proportion of <1000Da fraction was more than 60%. For the biochemical properties, hydrolysis

Shiyuan Dong; Mingyong Zeng; Dongfeng Wang; Zunying Liu; Yuanhui Zhao; Huicheng Yang

2008-01-01

202

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells  

Microsoft Academic Search

Background  Oxidative damage to the retinal pigment epithelium might be involved in the pathogenesis of age related macular degeneration.\\u000a Thus antioxidative protection represents a rationale for a causative therapy or prophylaxis. The aim of the present study\\u000a is to evaluate antioxidative properties of vitamin C and pyruvate at retinal pigment epithelial (RPE) cells exposed to oxidative\\u000a stress.\\u000a \\u000a \\u000a \\u000a Methods  The ability of vitamin

Oliver Zeitz; Lars Schlichting; Gisbert Richard; Olaf Strauß

2007-01-01

203

Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year  

PubMed Central

Background There is no available information on physicochemical and antioxidant properties on Bangladeshi honey. We investigated five different monofloral and three different multifloral honey samples collected from different parts of Bangladesh. Methods The levels of phenolics, flavonoids, ascorbic acid, ascorbic acid equivalent antioxidant content (AEAC), proline, protein and antioxidants were determined in the honey samples using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Results The highest level of phenolic was 688.5?±?5.9 mg Gallic acid/kg, and the highest level of flavonoid was 155?±?6.9 mg Catechin/kg. The highest color intensity was 2034.00?±?17.5 mAU, and the highest protein content was 8.6?±?0.0mg/g. High levels of proline (2932.8?±?3.7 mg/kg), ascorbic acid (154.3?±?0.3 mg/kg), AEAC (34.1?±?1.4mg/100 g) and FRAP (772.4?±?2.5 ?mol Fe (II)/100 g) were detected in some of the samples, especially the multifloral honey samples, indicating good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH, FRAP and color intensity, indicating that in addition to total phenolic and flavonoid concentrations, color intensity and amino acid are good indicators of the antioxidant potential of honey. Except for a single sample (BDH-6), the honey samples stored for 1.5 years at room temperature still had 5-hydroxymethylfurfural (HMF) values within the recommended range (mean = 10.93 mg/kg), indicating that the rate of HMF production in Bangladeshi honey samples is low. Conclusion It is postulated that the low rate of HMF formation could be attributed to the acidic and low moisture content in the samples. In general, multifloral honeys have higher antioxidant properties based on their high levels of phenolics, flavonoids, AEAC, DPPH and FRAP when compared to monofloral honeys. We also found that monofloral honey samples from Guizotia abyssinica and Nigella sativa had high antioxidant properties.

2012-01-01

204

Chemical characteristics and antioxidant properties of crude water soluble polysaccharides from four common edible mushrooms.  

PubMed

Four crude water soluble polysaccharides, CABP, CAAP, CFVP and CLDP, were isolated from common edible mushrooms, including Agaricus bisporus, Auricularia auricula, Flammulina velutipes and Lentinus edodes, and their chemical characteristics and antioxidant properties were determined. Fourier Transform-infrared analysis showed that the four crude polysaccharides were all composed of ?-glycoside linkages. The major monosaccharide compositions were D-galactose, D-glucose and D-mannose for CABP, CAAP and CLDP, while CFVP was found to consist of L-arabinose, D-galactose, D-glucose and D-mannose. The main molecular weight distributions of CABP and the other three polysaccharides were <5.1 × 10(4) Da and >66.0 × 10(4) Da, respectively. Antioxidant properties of the four polysaccharides were evaluated in in vitro systems and CABP showed the best antioxidant properties. The studied mushroom species could potentially be used in part of well-balanced diets and as a source of antioxidant compounds. PMID:22495548

He, Jin-Zhe; Ru, Qiao-Mei; Dong, Dan-Dan; Sun, Pei-Long

2012-04-11

205

Hypolipidemic and antioxidant properties of a polysaccharide fraction from Enteromorpha prolifera.  

PubMed

A polysaccharide fraction (EPF2) was obtained from the crude polysaccharides of Enteromorpha prolifera by a series isolation procedure. Monosaccharide components analysis indicated that EPF2 was composed of rhamnose, xylose, mannose, galactose and glucose in a molar ratio of 3.64:1.08:0.21:0.75:0.27. Hypolipidemic and antioxidant properties of EPF2 were investigated. The results showed that the hypolipidemic effect of EPF2 was in a concentration-dependent fashion and the prior oral administration of EPF2 (300 mg/kg body weight) exhibited considerable effect which could bear comparison with that of simvastatin. Moreover, administration of EPF2 could significantly enhance the activities of endogenous antioxidant enzymes and lowered the content of maleic dialdehyde (MDA) in serum. The results suggested that EPF2 had a high hypolipidemic activity and this activity might be attributed to its antioxidant potential. PMID:23541551

Tang, Zhihong; Gao, Hongwei; Wang, Shuai; Wen, Shaohong; Qin, Song

2013-03-26

206

Quality and antioxidant properties of breads enriched with dry onion (Allium cepa L.) skin.  

PubMed

The aim of the study was to investigate the effect on the antioxidant properties and sensory value of bread of adding ground onion skin (OS). For a determination of bioaccessibility and bioavailability in vitro the human gastrointestinal tract model was used. OS contained mastication-extractable quercetin (4.6 mg/g). Quercetin from OS was highly bioaccessible during in vitro conditions, but only approximately 4% of quercetin released during simulated digestion was bioavailable in vitro. The antioxidant potential of bread with OS was significantly higher than the activity noted in the control. In particular, OS addition significantly fortificated bread with bioaccessible lipid oxidation preventers and compounds with reducing and chelating abilities. The 2-3% OS addition caused significant improvement of antioxidant abilities (further increases in the OS supplement did not increase the activity of bread). Sensory evaluation showed that replacement of wheat flour in bread with up to 3% OS powder gave satisfactory consumer acceptability. PMID:23411290

Gawlik-Dziki, Urszula; ?wieca, Micha?; Dziki, Dariusz; Baraniak, Barbara; Tomi?o, Justyna; Czy?, Jaros?aw

2012-11-15

207

Comparative Study on the Chemical Composition, Antioxidant Properties and Hypoglycaemic Activities of Two Capsicum annuum L. Cultivars ( Acuminatum small and Cerasiferum )  

Microsoft Academic Search

The present study aimed to evaluate for the first time the phenols, flavonoids, carotenoids, capsaicin and dihydrocapsaicin\\u000a content and the antioxidant and hypoglycemic properties of Capsicum annuum var. acuminatum small and C. annuum var. cerasiferum air-dried fruits. The ethanol extract of C. annuum var. acuminatum small, characterized by the major content of total poliphenols, flavonoids, carotenoids and capsaicinoids, showed the

Rosa Tundis; Monica R. Loizzo; Federica Menichini; Marco Bonesi; Filomena Conforti; Giancarlo Statti; Damiano De Luca; Bruno de Cindio; Francesco Menichini

208

Changes in chemical composition and antioxidative properties of rye ginger cakes during their shelf-life.  

PubMed

Changes in chemical composition and antioxidative properties of rye ginger cakes during their shelf-life were investigated in this study. In particular, the changes in antioxidants content, antioxidative and reducing capacity, and Maillard reaction development in rye ginger cakes after long-term storage were addressed. Ginger cakes produced according to the traditional and current recipe were stored for 5 years at room temperature in a dark place. The total phenolic compounds (TPC), inositol hexaphosphate (IP6), reduced (GSH) and oxidised glutathione (GSSG) contents, antioxidant and reducing capacity and Maillard reaction products (MRPs) were determined in ginger cakes after storage and then compared to those measured after baking. After long-term storage a decrease in TPC and IP6 contents in cakes was noted. In contrast, an increase in antioxidative and reducing capacity of stored cakes was observed. Long-term storage induced formation of furosine, advanced and final Maillard reaction products and caused changes in both reduced and oxidised forms of glutathione. After long-term storage the modest changes in furosine, FAST index and browning in ginger cake formulated with dark rye flour may suggest that this product is the healthiest among others. Therefore, traditional rye ginger cakes can be considered as an example of a healthy food that is also relatively stable during long term storage as noted by the small chemical changes observed in its composition. PMID:22980898

Zieli?ski, Henryk; del Castillo, Maria Dolores; Przygodzka, Ma?gorzata; Ciesarova, Zuzana; Kukurova, Kristina; Zieli?ska, Danuta

2012-07-14

209

The photoprotective and antioxidative properties of luteolin are synergistically augmented by tocopherol and ubiquinone.  

PubMed

Ultraviolet radiation induces DNA damage and oxidative stress which can result in skin inflammation, photoaging, and photocarcinogenesis. The flavonoid luteolin that is present in high amounts in the dyers weld, Reseda luteola, is one of the most potent antioxidative plant metabolites and also has ultraviolet-absorbing properties.The aim of this study was to determine whether tocopherol and ubiquinone add synergistic antioxidative values to luteolin. None of the substances showed cytotoxic effects in concentrations from 0.25 to 4 µg/mL. The photoprotective and antioxidant effect of equivalent concentrations of luteolin, tocopherol, and ubiquinone and their combination in a ratio of 4 : 4 : 1 were studied in solar simulator irradiated human skin fibroblasts. Luteolin had a half-maximal radical scavenging concentration of 2 µg/mL, whereas tocopherol and ubiquinone were only effective at higher concentrations. None of the substances showed a phototoxic effect, and only luteolin had a moderate photoprotective effect at 2 µg/mL. The combination of luteolin, tocopherol, and ubiquinone exerted a synergistic radical scavenging effect already at a concentration of 0.25 µg/mL and a complete photoprotection at 2 µg/mL.In summary, our findings suggest that the potent antioxidant and photoprotective effect of flavonoids like luteolin may be further increased by the addition of low concentrations of other antioxidants such as tocopherol and ubiquinone. PMID:23839819

Wölfle, Ute; Haarhaus, Birgit; Schempp, Christoph M

2013-07-09

210

Evaluation of antioxidant and antimicrobial properties of Soymida febrifuga leaf extracts.  

PubMed

The present study was designed to evaluate the antioxidant and antimicrobial properties of hexane (LH), methanol (LM) and aqueous (LA) extracts of Soymida febrifuga (Maliaceae) leaves, which is a traditional folk medicine in India. No pharmacological evaluation of this plant (except antiplasmodial activity) has been reported to date. Antioxidant activity of different extracts was evaluated by DPPH free radical scavenging activity, taking total phenolic content (TPC) as an index. Antimicrobial activity was tested against six bacterial and five fungal strains using the agar hole diffusion method and the minimum inhibitory concentrations (MIC) and minimum microbicidal concentration (MMC) were determined for all the test organisms against the extracts. The results showed that the methanol and aqueous extracts of leaf had a higher antioxidant activity and total phenolic content than the hexane extract. The antioxidant activity and TPC of the extracts were highly correlated. Extracts also showed several degrees of antimicrobial activity against different microbes. The methanol extract was more potent against Aspergillus fumigatus and Candida tropicana. The lowest MIC values obtained for LM, LA and LH were 78, 156, 625 microg/mL against A. fumigatus, C. tropicana and C. albicans, respectively. Hence, this study confirms that Soymida febrifuga leaves possess potent antioxidant and antimicrobial activity. PMID:18389470

Reddy, Boreddy Srinivas; Reddy, Boreddy Purushotham; Raghavulu, Sapireddy Veer; Ramakrishna, Sistla; Venkateswarlu, Y; Diwan, Prakash V

2008-07-01

211

Antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate Schiff base ligands and their metal complexes  

NASA Astrophysics Data System (ADS)

In this study, two Schiff base ligands (HL 1 and HL 2) and their Cu(II), Co(II), Ni(II), Pd(II) and Ru(III) metal complexes were synthesized and characterized by the analytical and spectroscopic methods. Alkane oxidation activities of the metal complexes were studied on cyclohexane as substrate. The ligands and their metal complexes were evaluated for their antimicrobial activity against Corynebacterium xerosis, Bacillus brevis, Bacillus megaterium, Bacillus cereus, Mycobacterium smegmatis, Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis (as Gram-positive bacteria) and Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Yersinia enterocolitica, Klebsiella fragilis, Saccharomyces cerevisiae, and Candida albicans (as Gram-negative bacteria). The antioxidant properties of the Schiff base ligands were evaluated in a series of in vitro tests: 1,1-diphenyl-2-picrylhydrazyl (DPPH rad ) free radical scavenging and reducing power activity of superoxide anion radical generated non-enzymatic systems. Electrochemical and thermal properties of the compounds were investigated.

Ceyhan, Gökhan; Çelik, Cumali; Uru?, Serhan; Demirta?, ?brahim; Elmasta?, Mahfuz; Tümer, Mehmet

2011-10-01

212

Antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate Schiff base ligands and their metal complexes.  

PubMed

In this study, two Schiff base ligands (HL(1) and HL(2)) and their Cu(II), Co(II), Ni(II), Pd(II) and Ru(III) metal complexes were synthesized and characterized by the analytical and spectroscopic methods. Alkane oxidation activities of the metal complexes were studied on cyclohexane as substrate. The ligands and their metal complexes were evaluated for their antimicrobial activity against Corynebacterium xerosis, Bacillus brevis, Bacillus megaterium, Bacillus cereus, Mycobacterium smegmatis, Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis (as gram-positive bacteria) and Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Yersinia enterocolitica, Klebsiella fragilis, Saccharomyces cerevisiae, and Candida albicans (as gram-negative bacteria). The antioxidant properties of the Schiff base ligands were evaluated in a series of in vitro tests: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and reducing power activity of superoxide anion radical generated non-enzymatic systems. Electrochemical and thermal properties of the compounds were investigated. PMID:21752697

Ceyhan, Gökhan; Çelik, Cumali; Uru?, Serhan; Demirta?, ?brahim; Elmasta?, Mahfuz; Tümer, Mehmet

2011-06-21

213

Antioxidant power, bacteriostatic activity, and characterization of white grape pomace extracts by HPLC–ESI–MS  

Microsoft Academic Search

Polyphenolic fractions obtained from white grape pomace by aqueous extraction were analyzed to determine their antioxidant\\u000a power (DPPH and TBARS methods), bacteriostatic activity, the effect of particle size on total extractable polyphenols (expressed\\u000a as gallic acid equivalents (GAE)), and to characterize by HPLC–ESI–MS. The extraction of polyphenols from Albariño variety\\u000a grape pomace was performed both on lab-scale and on pilot-plant

Marivel Sánchez; Daniel Franco; Jorge Sineiro; Beatriz Magariños; Ma. José Núñez

2009-01-01

214

Subcritical water extraction of nutraceuticals with antioxidant activity from oregano. Chemical and functional characterization  

Microsoft Academic Search

In the present work, oregano leaves (Origanum vulgare L.) are explored as natural source of nutraceuticals with antioxidant activity. To do this, subcritical water extraction (SWE), a new environmentally friendly technique, is employed as extraction procedure and HPLC coupled to DAD is used for the chemical characterization of the extracts. Moreover, the radical scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the determination

I. Rodríguez-Meizoso; F. R. Marin; M. Herrero; F. J. Señorans; G. Reglero; A. Cifuentes; E. Ibáñez

2006-01-01

215

Antioxidant properties of Australian canola meal protein hydrolysates.  

PubMed

Antioxidant activities of canola protein hydrolysates (CPHs) and peptide fractions prepared using five proteases and ultrafiltration membranes (1, 3, 5, and 10kDa) were investigated. CPHs had similar and adequate quantities of essential amino acids. The effective concentration that scavenged 50% (EC50) of the ABTS(+) was greatest for the <1kDa pancreatin fraction at 10.1?g/ml. CPHs and peptide fractions scavenged DPPH(+) with most of the EC50 values being <1.0mg/ml. Scavenging of superoxide radical was generally weak, except for the <1kDa pepsin peptide fraction that had a value of 51%. All CPHs inhibited linoleic acid oxidation with greater efficiency observed for pepsin hydrolysates. The oxygen radical absorbance capacity of Alcalase, chymotrypsin and pepsin hydrolysates was found to be better than that of glutathione (GSH) (p<0.05). These results show that CPHs have the potential to be used as bioactive ingredients in the formulation of functional foods against oxidative stress. PMID:24176374

Alashi, Adeola M; Blanchard, Christopher L; Mailer, Rodney J; Agboola, Samson O; Mawson, A John; He, Rong; Girgih, Abraham; Aluko, Rotimi E

2013-09-25

216

Effects of baking conditions, dough fermentation, and bran particle size on antioxidant properties of whole-wheat pizza crusts.  

PubMed

This study investigated the effects of processing conditions including bran particle size, dough fermentation time, and baking time and temperature on the extractable antioxidant properties of whole-wheat pizza crust. Experiments were carried out using two different varieties of hard white winter wheat, Trego and Lakin. Antioxidant properties examined included oxygen radical absorbing capacity (ORAC), hydroxyl radical scavenging capacity (HOSC), relative 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity (RDSC), cation 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging capacity, total phenolic contents (TPC), and ferulic acid contents. Results indicated that bran particle size had no effect on the antioxidant properties evaluated. Increasing dough fermentation time from 0 to 48 h had no significant influence on antioxidant properties except HOSC, which increased as much as 28%, possibly as a result of increase in soluble free ferulic acid, which increased as much as 130%. Increasing baking temperature from 204 to 288 degrees C with a 7 min bake time increased all evaluated antioxidant properties by as much as 82%. Increasing baking time from 7 to 14 min with 204 degrees C baking temperature might increase some antioxidant properties as much as 60%. The results from this study suggest that longer dough fermentation times and increased baking time or temperature may be potential approaches to increase the antioxidant availability in whole-wheat pizza crust. PMID:19138086

Moore, Jeffrey; Luther, Marla; Cheng, Zhihong; Yu, Liangli Lucy

2009-02-11

217

Effect of antioxidant properties of lecithin emulsifier on oxidative stability of encapsulated bioactive compounds.  

PubMed

Oxidation of encapsulated bioactive compounds in emulsions is one of the key challenges that limit shelf life of emulsion containing products. Oxidation in these emulsions is triggered by permeation of free radicals generated at the emulsion interface. The objective of this study was to evaluate the role of antioxidant properties of common emulsifiers (lecithin and Tween 20) in reducing permeation of free radicals across the emulsion interface. Radical permeation rates were correlated with oxidative stability of a model bioactive compound (curcumin) encapsulated in these emulsions. Rate of permeation of peroxyl radicals from the aqueous phase to the oil phase of emulsion was inversely proportional to the antioxidant properties of emulsifiers. The rate of radical permeation was significantly higher (p<0.05) for emulsions stabilized using Tween 20 and oxidized lecithin compared to native lecithin that showed higher antioxidant activity. Free radical permeation rate correlated with stability of curcumin in emulsions and was significantly higher (p<0.05) in lecithin stabilized emulsions as compared to Tween 20 emulsions. Overall, this study demonstrates that antioxidant activity of emulsifiers significantly influences permeation of free radicals across the emulsion interface and the rate of oxidation of bioactive encapsulant. PMID:23618963

Pan, Yuanjie; Tikekar, Rohan V; Nitin, N

2013-04-22

218

Antioxidant properties and polyphenolic compositions of fruits from different European cranberrybush (Viburnum opulus L.) genotypes.  

PubMed

Antioxidant properties of fruit juices of six Viburnum opulus genotypes were evaluated by DPPH, ABTS(+) radical scavenging capacity (RSC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC) and Folin-Ciocalteu total phenolic content (TPC) assays. TPC varied in the range of 5.4-10.6 mg gallic acid equivalents/g, RSC (ABTS(+)), FRAP and ORAC values were 31.9-109.8, 32.3-61.8 and 141.6-260.4 ?mol trolox equivalents/g, respectively. V. opulus var. sargentii fruit juice was a remarkably stronger antioxidant than the other five V. opulus genotypes. The content of chlorogenic acid (the main phenolic compound in berry juices) depending on plant cultivar varied in the range of 0.54-6.93 mg/ml. The RSC of individual constituents was measured by the on-line HPLC-UV-DPPH method: chlorogenic acid was the dominant radical scavenger in V. opulus P3 (74%), while epicatechin and catechin (the main antioxidants in V. opulus var. sargentii) contributed to 40% and 23% of the total RSC for the sargentii genotype. Nine constituents were identified in V. opulus juice by using ultra high performance liquid chromatography coupled to quadruple and time-of-flight mass spectrometers (UPLC-QTOF-MS). In general, the study demonstrated that V. opulus var. sargentii followed by V. opulus P3 and V. opulus var. americanum possessed the highest antioxidant capacity. The obtained results may assist in selecting the most valuable V. opulus genotypes for the production of fruits possessing strong antioxidant capacity and containing beneficial phenolic constituents. PMID:23993538

Kraujalyt?, Vilma; Venskutonis, Petras Rimantas; Pukalskas, Audrius; ?esonien?, Laima; Daubaras, Remigijus

2013-06-20

219

Water vapor permeability, mechanical properties and antioxidant effect of Mexican oregano-soy based edible films.  

PubMed

Water-soluble extracts from Mexican oregano (Lippia graveolens) were incorporated into soy protein isolate (SPI) films. Water vapor permeability, mechanical properties, and antioxidant ability were evaluated. All the extracts were capable of scavenging DPPH radicals in a concentration-dependent fashion; the IC50 values were obtained. Oregano extracts were incorporated into SPI films plasticized with sorbitol, glycerol, and glycerol-sorbitol 1:1. The addition of the extracts resulted in an increase in the water vapor permeability values and provided a dark reddish film appearance. Changes in tensile strength as well as elongation values were observed. The oregano SPI films exhibited antioxidant properties in a concentration-dependent fashion. PMID:19241539

Pruneda, E; Peralta-Hernández, J M; Esquivel, K; Lee, S Y; Godínez, L A; Mendoza, S

2008-08-01

220

Structure-property studies on the antioxidant activity of flavonoids present in diet.  

PubMed

The screening of natural flavonoids for their bioactivity as antioxidants is usually carried out by determinination of their profile as chain-breaking antioxidants, by the evaluation of their direct free radical-scavenging activity as hydrogen- or electron-donating compounds. Since this may not be the only mechanism underlying the antioxidant activity it is important to check the ability of these compounds to act as chelators of transition metal ions. Accordingly, in the present study the acidity constants of catechin and taxifolin, as well as the formation constants of the corresponding copper (II) complexes, were investigated by potentiometry and/or spectrophotometry. Moreover, a detailed quantitative examination of the coordination species formed is presented. In addition, the partition coefficients of both catechin and taxifolin in a biomimetic system (micelles) were determined, since these properties may also contribute to the antioxidant behavior of this type of compound. The log P values determined depend on the electrostatic interactions of the compounds with the differently charged micelles (the highest values were obtained for zwitterionic and cationic micelles). The prooxidant behavior of the compounds was assessed through the oxidation of 2'-deoxyguanosine, induced by a Fenton reaction, catalyzed by copper. The data obtained reveal that the flavonoids under study did not present prooxidant activity, in this particular system. The results obtained are evidence of a clear difference among the pKa, the complexation properties, and the lipophilicity of the flavonoids studied, which can partially explain their distinct antioxidant activity. The most stable geometries of the free compounds were determined by theoretical (ab initio) methods, in order to properly account for the electron correlation effects which occur in these systems, thus allowing a better interpretation of the experimental data. PMID:16198236

Teixeira, Susana; Siquet, Christophe; Alves, Carla; Boal, Isabel; Marques, M Paula; Borges, Fernanda; Lima, José L F C; Reis, Salette

2005-10-15

221

Analysis method and characterization of the antioxidant capacity of vitamin E-interactive polysulfone hemodialyzers.  

PubMed

The lipophilic antioxidant vitamin E was used as a surface modifier (or coating agent) of hollow-fiber hemodialyzer membranes with the aim of increasing their biocompatibility and preventing oxidative stress, which are the main clinical drawbacks in hemodialysis (HD) therapy. At present, the redox chemistry of vitamin E-modified dialyzers is not well characterized and there is no standard method to assess the antioxidant capacity of these biomembranes under conditions that simulate those observed during HD therapy. With this study, we developed an original online method to determine the antioxidant capacity of vitamin E-modified dialyzer membranes during circulation experiments. This method is based on a spectrophotometric assay known as the ferric reducing/antioxidant power assay (FRAP). The principle of FRAP and its application to the qualitative and quantitative assessment of miniaturized polysulfone (PS)-based vitamin E-modified dialyzers (PS-VE) were verified by the accurate in vitro analysis of the iron-catalyzed oxidation of vitamin E. The antioxidant capacity of miniaturized PS-VE samples assessed in this study was of 14.5 microM Fe(2+), which corresponded to the transformation of nearly one-third of the vitamin E bound to the hollow-fiber membrane to its oxidation end product alpha-tocopherol quinone. This method shows good reproducibility and intra- and inter-assay precision, and can be easily adapted to determine the redox activity of every type of vitamin E-modified dialyzers during technological investigation, manufacturing control and clinical research. PMID:19442768

Floridi, Alessandro; Piroddi, Marta; Pilolli, Francesca; Matsumoto, Yutaka; Aritomi, Masaharu; Galli, Francesco

2009-05-04

222

Antioxidant and Free Radical Scavenging Properties of Developed Mono and Polyherbal Formulations  

Microsoft Academic Search

The antioxidant properties of developed mono and polyherbal tablet formulations viz. Withatab, Asparatab, Centab and Ascenwit that probably involve free radical mechanisms, were evaluated by the methods, namely the DPPH (1,1-diphenyl-2-picryl hydrazyl) free radical scavenging assay, Super oxide scavenging assay, Nitric oxide radical scavenging assay and ABTS (2, 2'-azinobis-3-ethyl-benzothiozoline-6-sulphonic acid) radical scavenging assay. The standardized methanol extract from Withania somnifera,

P. K. Jain; R. K. Agrawal

223

Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid  

Microsoft Academic Search

The antioxidant and pro-oxidant properties of ascorbic acid (AA) and gallic acid (GA) were investigated. AA and GA, at a concentration of 1.65 mM, accelerate the oxidation of deoxyribose induced by Fe3+–EDTAJH2O2. The reducing power of these two compounds increased upon increasing the concentration. AA and GA showed no chelating ability toward iron (II). At a concentration of 4.17 mM,

Gow-Chin Yen; Pin-Der Duh; Hui-Ling Tsai

2002-01-01

224

Antioxidant property of aroma extract isolated from clove buds [ Syzygium aromaticum (L.) Merr. et Perry  

Microsoft Academic Search

Aroma extract from dried clove buds [Syzygium aromaticum (L.) Merr. et Perry] was obtained by using steam-distillation under mild conditions (55°C and 95 mm Hg). The antioxidant property of the aroma extract was evaluated in two different assays. The aroma extract isolated from clove buds inhibited the oxidation of hexanal for 30 days at a level of 50 ?g\\/ml. Clove

Kwang-Geun Lee; Takayuki Shibamoto

2001-01-01

225

The impact of LED illumination on antioxidant properties of sprouted seeds  

Microsoft Academic Search

The objective of this study was to evaluate the effect of the light emitting diode (LED) spectra on the antioxidant properties\\u000a of sprouted wheat (Triticum aestivum L.), radish (Raphanus sativus L.), and lentil (Lens esculenta Moenh.) seeds. Lighting experiments were performed under controlled conditions (PPFD - 100 ?mol m?2 s?1; 12 h photoperiod; 27°C). The LED conditions used were: L1

Giedr? Samuolien?; Akvil? Urbonavi?i?t?; Aušra Brazaityt?; Gintar? Šabajevien?; Jurga Sakalauskait?; Pavelas Duchovskis

2011-01-01

226

Solidphase extraction of berries’ anthocyanins and evaluation of their antioxidative properties  

Microsoft Academic Search

Solid-phase extraction (SPE) was used to obtain anthocyanin-rich extracts from five berry species: chokeberry, elderberry, black currant, blackberry and blueberry. During SPE more than 94.4% of the sugars and more than 88.5% of the acids present in the crude extracts were separated. The SPE resulted in 90–95.6% anthocyanins recovery. The antioxidative properties of the anthocyanin-rich extracts were tested by measuring

Petko Denev; Milan Ciz; Gabriela Ambrozova; Antonin Lojek; Irina Yanakieva; Maria Kratchanova

2010-01-01

227

Antimutagenic and Antioxidant Properties of Phenolic Fractions from Andean Purple Corn ( Zea mays L.)  

Microsoft Academic Search

The antimutagenic and antioxidant properties of various phenolic fractions obtained from Andean purple corn were examined by the Ames test and the DPPH antiradical assay. An anthocyanin-rich water fraction (WF) and an ethyl acetate fraction (EAF) showed a dose-dependent antimutagenic behavior against the food mutagen Trp-P-1 with IC50 values of 321.7 ( 21.36 and 95.2 ( 10.95 Ìg of chlorogenic

Romina Pedreschi; Luis Cisneros-Zevallos

2006-01-01

228

Isolation and antioxidant property of the extracellular polysaccharide from Rhodella reticulata  

Microsoft Academic Search

The extracellular polysaccharide from Rhodella reticulata was separated from the culture medium followed by concentration and ethanol precipitation, and purified by anion exchange\\u000a chromatography on DEAE-Sepharose Fast Flow. This study compared the free radical-scavenging property and antioxidant activity\\u000a with various treatments of crude extracellular polysaccharides of R. reticulata. The results showed that both the crude extracellular polysaccharide and deproteinized crude

Bilian ChenWenlang; Wenlang You; Jian Huang; Ying Yu; Weiping Chen

2010-01-01

229

Antioxidant properties of Thonningianin A, isolated from the African medicinal herb, Thonningia sanguinea  

Microsoft Academic Search

The antioxidant properties of Thonningianin A (Th A), an ellagitannin, isolated from the methanolic extract of the African medicinal herb, Thonningia sanguinea were studied using the NADPH and Fe2+\\/ascorbate-induced lipid peroxidation (LPO), electron spin resonance spectrometer and the deoxyribose assay. Th A at 10?M inhibited both the NADPH and Fe2+\\/ascorbate-induced LPO in rat liver microsomes by 60% without inhibitory effects

Maxwell Afari Gyamfi; Yoko Aniya

2002-01-01

230

Nutritional constituents and antioxidant properties of indigenous kembayau ( Dacryodes rostrata (Blume) H. J. Lam) fruits  

Microsoft Academic Search

The nutritional and antioxidant properties of peels, pulp and seeds of kembayau (Dacryodes rostrata) fruits were evaluated. Kembayau seeds and pulp were rich in fat, while peels had the highest ash contents. Potassium was the most prevalent mineral in peels (380.72–1112.00mg\\/100g). In kembayau fruits, total flavonoid content (1012.74–28,022.28mg rutin equivalent\\/100g) was higher than total phenolic and total monomeric anthocyanin contents.

Kin Weng Kong; Lye Yee Chew; K. Nagendra Prasad; Cheng Yuon Lau; Amin Ismail; Jian Sun; Bahareh Hosseinpoursarmadi

2011-01-01

231

Antioxidant and Lysosomotropic Properties of Acridine-propranolol: Protection against Oxidative Endothelial Cell Injury  

Microsoft Academic Search

The antioxidant and lysosomotropic properties of a fluorescent analogue of propranolol, 9-amino-acridine-propranolol (9-AAP) were compared to those of propranolol. Using isolated microsomal membranes exposed to a superoxide and hydroxyl radical generating system, 9-AAP was found to be at least 10-fold more potent than propranolol (and about 50% as potent as vitamin E) in inhibiting lipid peroxidation. In cultured endothelial cells,

Benjamin F. Dickens; William B. Weglicki; Patricia A. Boehme; Tong I. Mak

2002-01-01

232

Investigations of the antioxidant properties of plant extracts using a DNA-electrochemical biosensor  

Microsoft Academic Search

In this work, the results of a method based on an electrochemical biosensor to detect DNA damage in vitro for the evaluation of the antioxidant properties of plant extracts are reported. The biosensor consisted of a dsDNA immobilized on a screen-printed electrode surface (SPE). DNA damage was promoted by the generation of the OH radicals via Fenton-type reaction. The interaction

Lucilene Dornelles Mello; Silvia Hernandez; Giovanna Marrazza; Marco Mascini; Lauro Tatsuo Kubota

2006-01-01

233

Compositions, functional properties and antioxidative activity of protein hydrolysates prepared from round scad ( Decapterus maruadsi)  

Microsoft Academic Search

Composition, functional properties and antioxidative activity of a protein hydrolysate prepared from defatted round scad (Decapterus maruadsi) mince, using Flavourzyme, with a degree of hydrolysis (DH) of 60%, were determined. The protein hydrolysate had a high protein content (48.0%) and a high ash content (24.56%). It was brownish yellow in colour (L?=58.00, a?=8.38, b?=28.32). The protein hydrolysate contained a high

Yaowapa Thiansilakul; Soottawat Benjakul; Fereidoon Shahidi

2007-01-01

234

Hypolipidemic and antioxidant properties of Ganoderma lucidum (Leyss:Fr) Karst used as a dietary supplement  

Microsoft Academic Search

In this study, the hypolipidemic and antioxidant properties of Ganoderma lucidum CG 144, a medicinal mushroom cultivated on wet wheat grains by solid-state fermentation, were investigated followed dietary supplementation.\\u000a Basal chow was supplemented with 85, 50, or 10% of G. lucidum CG 144 dried spawn, resulting in G85, G50, and G10 diets, respectively, and fed to normocholesterolemic and induced-hypercholesterolemic\\u000a mice.

Rosália Rubel; Herta S. Dalla Santa; Luiz Cláudio Fernandes; Sandro J. R. Bonatto; Sérgio Bello; Bonald C. Figueiredo; José Hermenio C. Lima Filho; Cid Aimbiré M. Santos; Carlos Ricardo Soccol

2011-01-01

235

Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions  

PubMed Central

Background Diabetes is a serious metabolic disorder affecting the metabolism of carbohydrate, protein and fat. A number of studies have shown that diabetes mellitus is associated with oxidative stress, leading to an increased production of reactive oxygen species. Ficus deltoidea is traditionally used in Malaysia for regulating blood sugar, blood pressure and cholesterol levels. The use of F. deltoidea as an alternative medicinal herb is increasingly gaining popularity with the sale of F. deltoidea tea bags and capsules in the local market. The present study was undertaken to investigate the antidiabetic and antioxidant activities of the fruits from different varieties of F. deltoidea, employing in vitro methods. Method Two fruit varieties of F. deltoidea (var. angustifolia (SF) and var. kunstleri (BF)) were extracted separately using double-distilled water. The resulting aqueous extracts were partitioned using ethyl acetate to obtain the ethyl acetate and water fractions. The crude aqueous extracts and the corresponding fractions were evaluated for their phenolic, flavonoid, sugar and protein contents. Protein profiling of the extracts and fractions were also carried out by means of SDS-PAGE and SELDI-TOF MS. Antidiabetic activities were assessed based on the ability of the samples to inhibit yeast and mammalian ?-glucosidase as well as ?-amylase. Antioxidant capacities were examined by measuring the ability of the samples to reduce ferric ions and to scavenge DPPH, superoxide anion, ABTS and nitric oxide radicals. Results The crude extracts and fractions of SF and BF inhibited both yeast and rat intestinal ?-glucosidases in a dose-dependent manner, but did not inhibit porcine pancreatic ?-amylase. The water fraction of BF showed the highest percentage of ?-glucosidase inhibition while having the highest amount of protein (73.33?±?4.99 ?g/mg fraction). All the extracts and fractions exhibited antioxidant activities, with SF crude extract showing the highest antioxidant activity and phenolic content (121.62?±?4.86 mg/g extract). Fractionation of the crude extracts resulted in loss of antioxidant activities. There was no positive correlation between phenolic and flavonoid content with ?-glucosidase inhibitory activities. However, phenolic content correlated well with antioxidant activities of the crude extracts but not with the fractions. Conclusions The antioxidant activities of the fruits of F. deltoidea might be asserted by the phenolic content but other polar plant components were possibly involved in the antidiabetic properties. The study of these compounds having both antihyperglycemic and antioxidant activities may provide a new approach in the treatment of diabetes mellitus.

2013-01-01

236

Antimicrobial property, antioxidant capacity, and cytotoxicity of essential oil from cumin produced in Iran.  

PubMed

Cumin (Cuminum cyminum) is one of the commonly used spices in food preparations. It is also used in traditional medicine as a stimulant, a carminative, and an astringent. In this study, we characterized the antimicrobial, antioxidant, and cytotoxic activities of cumin. E. coli, S. aureus, and S. faecalis were sensitive to various oil dilutions. The total phenol content of the essential oil was estimated to be 33.43 microg GAE/mg of the oil. The oil showed higher antioxidant activity compared with that of BHT and BHA. The cumin essential oil exhibited a dose-dependent scavenging of DPPH radicals and 5.4 microg of the oil was sufficient to scavenge 50% of DPPH radicals/mL. At a concentration of 0.1 microL/mL, oil destructed Hela cells by 79%. The antioxidant activity of cumin essential oil might contribute to its cytotoxic activity. Acute and subchronic toxicity was studied in a 30-d oral toxicity study by administration to Wistar rats of the essential oil. A 17.38% decrease in WBCs count, and 25.77%, 14.24%, and 108.81% increase in hemoglobin concentration, hematocrit, and platelet count, respectively, were noted. LDL/HDL ratio was reduced to half, which adds to the nutritional effects of cumin. Thus, cumin with a high phenolic content and good antioxidant activity can be supplemented for both nutritional purposes and preservation of foods. PMID:20492235

Allahghadri, Tolou; Rasooli, Iraj; Owlia, Parviz; Nadooshan, Mohammadreza Jalali; Ghazanfari, Tooba; Taghizadeh, Massoud; Astaneh, Shakiba Darvish Alipoor

2010-03-01

237

Antioxidant Properties of Water-Soluble Fullerene Derivatives  

Microsoft Academic Search

\\u000a Due to their inherent electronic properties, fullerenes are considered as radical sponges being capable of effectively quenching\\u000a reactive oxygen species (ROS). The most promising candidates for potential pharmaceutical applications are therefore water-soluble\\u000a fullerene derivatives, since they provide reasonable biological availability. In light of these considerations, we give an\\u000a overview over the most recent concepts for designing and synthesizing real water-soluble

Florian Beuerle; Russell Lebovitz; Andreas Hirsch

2008-01-01

238

Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties.  

PubMed Central

The flavonoids constitute a large group of polyphenolic phytochemicals with antioxidant properties in vitro. The interactions of four structurally related flavonoids (quercetin, kaempferol, rutin and luteolin) with Cu2+ ions were investigated in terms of the extent to which they undergo complex formation through chelation or modification through oxidation, as well as in their structural dependence. The ortho 3',4'-dihydroxy substitution in the B ring is shown to be important for Cu2+-chelate formation, thereby influencing the antioxidant activity. The presence of a 3-hydroxy group in the flavonoid structure enhances the oxidation of quercetin and kaempferol, whereas luteolin and rutin, each lacking the 3-hydroxy group, do not oxidize as readily in the presence of Cu2+ ions. The results also demonstrate that the reactivities of the flavonoids in protecting low-density lipoprotein (LDL) against Cu2+ ion-induced oxidation are dependent on their structural properties in terms of the response of the particular flavonoid to Cu2+ ions, whether chelation or oxidation, their partitioning abilities between the aqueous compartment and the lipophilic environment within the LDL particle, and their hydrogen-donating antioxidant properties.

Brown, J E; Khodr, H; Hider, R C; Rice-Evans, C A

1998-01-01

239

Antioxidant and Hepatoprotective Properties of Tofu (Curdle Soymilk) against Acetaminophen-Induced Liver Damage in Rats.  

PubMed

The antioxidant and hepatoprotective properties of tofu using acetaminophen to induce liver damage in albino rats were evaluated. Tofus were prepared using calcium chloride, alum, and steep water as coagulants. The polyphenols of tofu were extracted and their antioxidant properties were determined. The weight gain and feed intake of the rats were measured. The analysis of serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities and the concentrations of albumin, total protein, cholesterol, and bilirubin were analyzed. The result reveals that the antioxidant property of both soluble and bound polyphenolic extracts was significantly higher in all tofus, but the steep water coagulated tofu was recorded higher. Rats fed with various tofus and acetaminophen had their serum ALP, ALT, AST, and LDH activities; total cholesterol; and bilirubin levels significantly (P < 0.05) reduced, and total protein and albumin concentrations increased when compared with basal diet and acetaminophen administered group. Therefore, all tofus curdled with various coagulants could be used to prevent liver damage caused by oxidative stress. PMID:23533782

Yakubu, Ndatsu; Oboh, Ganiyu; Olalekan, Amuzat Aliyu

2013-02-28

240

Sulfur free radical reactivity with curcumin as reference for evaluating antioxidant properties of medicinal zingiberales.  

PubMed

Rhizome extracts of some members of the medicinal Zingiberales are widely used in dietary intake as well as in the traditional system of medicine. Curcumin, the chrome orange-yellow coloring compound present in turmeric rhizomes, has long been known to possess antioxidant property. Crude methanol extracts of the rhizomes of 11 species--Alpinia allughas, A. galanga, Curcuma amada, C. caesia, C. leucorrhiza, Hedychium coronarium, H. coccineum, H. flavescens, Kaempferia galanga, Zingiber cassumunar, and Z. officinale--were evaluated for their antioxidant properties using sulfur free radical reactivity with curcumin as a reference indicator. Sulfur free radicals (GS.) are generated by irradiating 15 mM glutathione (GSH) solution using a 5100 Ci cobalt-60 gamma irradiator. As reference indicator for the reactivity with sulfur free radicals, we used the depletion of pure curcumin sample in vitro as determined by a simple spectrophotometric method. The addition of the supernatant from crude rhizome extracts to the reaction mixture significantly decreased the depletion of curcumin, indicating that these crude extracts possessed antioxidant properties. The relative curcumin protection by different crude extracts against GS. showed that Z. cassumunar gave the highest degree of radioprotection. Our results reveal the potential medicinal use of rhizomes of medicinal Zingiberales as dietary agents. PMID:15312045

Chirangini, Pukhrambam; Sharma, Gurumayum J; Sinha, Swapan K

2004-01-01

241

Impact of solar dehydration on composition and antioxidant properties of acai (Euterpe oleracea Mart.).  

PubMed

Commercial products derived from the acai fruit (Euterpe oleracea Mart.) are available in Brazil, but in Venezuela, it is only known by ethnic indigenous groups of the Amazon. In this study, acai flour was made by solar dehydration and the effect of processing on the composition, microbiological quality, and antioxidant properties of such flour were evaluated. The fruit was purchased in Puerto Ayacucho, Venezuela, and a portion was manually pulped. Microbiological quality, proximal composition, minerals, polyphenols, tannins, anthocyanins, and antioxidant capacity were evaluated. The remaining portion of fruit was blanched in a solution of ascorbic acid and citric acid at 98 degrees C for 1 min in the same manner, manually pulped, dried by solar dehydration and the acai flour was also analysed. From the composition of the acai flour, its high content of fat (22.9%), protein (13.7%), dietary fibre (20.5%), total polyphenols (1.60 g/kg) and antioxidant capacity (79.97%) stood out. The blanching of the fruit and the solar dehydrating of the acai pulp did not modify the composition, but they improved its microbiological quality and reduced phenolic compounds and antioxidant capacity. The flour obtained is stable and innocuous and could be used to diversify the diet of the indigenous people of the Amazon region. PMID:22097293

Sangronis, Elba; Sanabria, Neida

2011-03-01

242

Evaluation of the antioxidant and antimicrobial properties of in vitro cultured Drosera intermedia extracts.  

PubMed

Evaluation of the antioxidant activity of the methanol, water and n-hexane extracts of Drosera intermedia, determined by the Folin-Ciocalteau (F-C), trolox equivalent antioxidant capacity (TEAC) and oxygen radical antioxidant capacity (ORAC) assays showed that the methanol extract had the highest antioxidant activity (F-C: 378.6 +/- 31.5 micromol(GAE)/mg(extract); TEAC: 332.2 +/- 29.1 micromol(TE)/mg(extract); ORAC: 64.7 +/- 7.8 micromol(TE)/mg(extract). Antimicrobial activity was tested against seven bacterial and eight yeast strains using the agar diffusion assay, followed by the determination of minimum inhibitory concentrations (MIC). All tested D. intermedia extracts demonstrated strong antimicrobial properties with a broad spectrum of activity. However, the n-hexane extract exhibited much greater activity than water and methanol extracts. The most susceptible microorganisms to the n-hexane extract were Staphylococcus epidermidis ATCC 12228 and Candida albicans YP0175, for which a MIC value of 13.0 microg/mL was scored. PMID:19768984

Grevenstuk, Tomás; Gonçalves, Sandra; Almeida, Sara; Coelho, Natacha; Quintas, Célia; Gaspar, Maria Nelma; Romano, Anabela

2009-08-01

243

Antioxidant Properties of Brazilian Tropical Fruits by Correlation between Different Assays  

PubMed Central

Four different assays (the Folin-Ciocalteu, DPPH, enzymatic method, and inhibitory activity on lipid peroxidation) based on radically different physicochemical principles and normally used to determine the antioxidant activity of food have been confronted and utilized to investigate the antioxidant activity of fruits originated from Brazil, with particular attention to more exotic and less-studied species (jurubeba, Solanum paniculatum; pequi, Caryocar brasiliense; pitaya, Hylocereus undatus; siriguela, Spondias purpurea; umbu, Spondias tuberosa) in order to (i) verify the correlations between results obtained by the different assays, with the final purpose to obtain more reliable results avoiding possible measuring-method linked mistakes and (ii) individuate the more active fruit species. As expected, the different methods give different responses, depending on the specific assay reaction. Anyhow all results indicate high antioxidant properties for siriguela and jurubeba and poor values for pitaya, umbu, and pequi. Considering that no marked difference of ascorbic acid content has been detected among the different fruits, experimental data suggest that antioxidant activities of the investigated Brazilian fruits are poorly correlated with this molecule, principally depending on their total polyphenolic content.

Pereira Lima, Giuseppina Pace; Fabris, Sabrina

2013-01-01

244

Evaluation of antioxidant activity and characterization of phenolic constituents of Phyllanthus amarus root.  

PubMed

The antioxidant property of the 70% aqueous ethanol extract of Phyllanthus amarus roots and its ether-soluble, ethyl acetate-soluble, and aqueous fractions were investigated by various in vitro assays. The root extracts showed higher DPPH, hydroxyl, superoxide, and nitric oxide radical scavenging and reducing power activity. Among all the samples, the ethyl acetate-soluble fraction demonstrated highest radical scavenging activity and total phenolics content. Twenty-eight different phenolic compounds were identified by LCMS/MS analysis of the ethyl acetate-soluble fraction. The majority of the compounds were found to exist as their glycosides, and many of these were gallic acid derivatives. Free epicatechin and gallic acid were also identified in the ethyl acetate-soluble fraction. The present investigation suggested that P. amarus root is a potent antioxidant and can be used for the prevention of diseases related to oxidative stress. PMID:23458355

Maity, Soumya; Chatterjee, Suchandra; Variyar, Prasad Shekhar; Sharma, Arun; Adhikari, Soumyakanti; Mazumder, Santasree

2013-03-25

245

Accelerated Characterization of Polymer Properties  

SciTech Connect

This report describes the efforts to develop a suite of microanalysis techniques that can rapidly measure a variety of polymer properties of industrial importance, including thermal, photo-oxidative, and color stability; as well as ductility, viscosity, and mechanical and antistatic properties. Additional goals of the project were to direct the development of these techniques toward simultaneous measurements of multiple polymer samples of small size in real time using non-destructive and/or parallel or rapid sequential measurements, to develop microcompounding techniques for preparing polymers with additives, and to demonstrate that samples prepared in the microcompounder could be analyzed directly or used in rapid off-line measurements. These enabling technologies are the crucial precursors to the development of high-throughput screening (HTS) methodologies for the polymer additives industry whereby the rate of development of new additives and polymer formulations can be greatly accelerated.

R. Wroczynski; l. Brewer; D. Buckley; M. Burrell; R. Potyrailo

2003-07-30

246

Phytochemical Analysis and Antioxidant Property of Leaf Extracts of Vitex doniana and Mucuna pruriens  

PubMed Central

Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of diverse disease states. The phytochemical screening and antioxidant property of fresh leaves of Vitex doniana and Mucuna pruriens, used in the management and treatment of various diseases, were studied. The extracts (ethanol and distilled water) were screened for the presence of phytochemicals, and their inhibition of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical was used to evaluate their free radical scavenging activity. Liver levels of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) in carbon tetrachloride- (CCl4) treated albino rats were also used to assess the antioxidant activity of the extracts. The animals were treated with 250?mg/kg body weight of the extracts for six consecutive days before a single dose (2.5?mL/kg body weight) of CCl4. Vitamin C was used as the standard antioxidant. Phytochemical screening revealed the presence of saponins, tannins, anthraquinones, terpenoids, and flavonoids in all the extracts, while alkaloids were detected in extracts of Vitex doniana only, and cardiac glycosides occurred in extracts of Mucuna pruriens only. All the extracts inhibited DPPH radical in a concentration-dependent manner, water extract of Vitex doniana producing highest inhibition which was not significantly different (P > .05) from vitamin C. The extracts produced a significant decrease (P < .05) in liver MDA, while the levels of SOD and CAT significantly increased (P < .05) relative to the positive control. These results are an indication of antioxidant potential of the extracts and may be responsible for some of the therapeutic uses of these plants.

Agbafor, K. N.; Nwachukwu, N.

2011-01-01

247

Phytochemical Analysis and Antioxidant Property of Leaf Extracts of Vitex doniana and Mucuna pruriens.  

PubMed

Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of diverse disease states. The phytochemical screening and antioxidant property of fresh leaves of Vitex doniana and Mucuna pruriens, used in the management and treatment of various diseases, were studied. The extracts (ethanol and distilled water) were screened for the presence of phytochemicals, and their inhibition of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical was used to evaluate their free radical scavenging activity. Liver levels of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) in carbon tetrachloride- (CCl(4)) treated albino rats were also used to assess the antioxidant activity of the extracts. The animals were treated with 250?mg/kg body weight of the extracts for six consecutive days before a single dose (2.5?mL/kg body weight) of CCl(4). Vitamin C was used as the standard antioxidant. Phytochemical screening revealed the presence of saponins, tannins, anthraquinones, terpenoids, and flavonoids in all the extracts, while alkaloids were detected in extracts of Vitex doniana only, and cardiac glycosides occurred in extracts of Mucuna pruriens only. All the extracts inhibited DPPH radical in a concentration-dependent manner, water extract of Vitex doniana producing highest inhibition which was not significantly different (P > .05) from vitamin C. The extracts produced a significant decrease (P < .05) in liver MDA, while the levels of SOD and CAT significantly increased (P < .05) relative to the positive control. These results are an indication of antioxidant potential of the extracts and may be responsible for some of the therapeutic uses of these plants. PMID:21547085

Agbafor, K N; Nwachukwu, N

2011-04-18

248

Anti-oxidant, pro-oxidant properties of tannic acid and its binding to DNA.  

PubMed

Tannic acid has numerous food and pharmacological applications. It is an additive in medicinal products, and is used as a flavouring agent and as an anti-oxidant in various foods and beverages. We have previously shown that tannic acid in the presence of Cu(II) causes DNA degradation through generation of reactive oxygen species. On the other hand, it exhibits antimutagenic and anticarcinogenic activities, and induces apoptosis in animal cells. It is known that most plant-derived polyphenolic anti-oxidants also act as pro-oxidants under certain conditions. In this paper, we compare the anti-oxidant and pro-oxidant properties of tannic acid and its structural component gallic acid. It is shown that tannic acid is the most efficient generator of the hydroxyl radical in the presence of Cu(II), as compared with gallic acid and its analogues syringic acid and pyrogallol. The anti-oxidant activity of tannic acid was studied by its effect on hydroxyl radical and singlet oxygen mediated cleavage of plasmid DNA. Again, tannic acid provided the maximum protection against cleavage, while gallic acid and its structural analogues were found to be non-inhibitory or partially inhibitory. The results suggest that the structural features of tannic acid that are important for its anti-oxidant action are also those that contribute to the generation of hydroxyl radicals in the presence of Cu(II). Restriction analysis of treated phage DNA and thermal melting profiles of calf thymus DNA indicated that tannic acid strongly binds to DNA. Indirect evidence indicates that modification of DNA bases may also occur. PMID:10731518

Khan, N S; Ahmad, A; Hadi, S M

2000-03-15

249

Synthesis, characterization and properties of novel BN nanocages from a single-source precursor  

NASA Astrophysics Data System (ADS)

In this Letter, we report the synthesis of hexagonal crystalline BN powders with novel cage-like structures from a single-source precursor NH 4BF 4 at 400-600 °C for 12 h. The obtained samples are characterized by XRD, XPS, TEM and HRTEM. Experiments reveal that the as-prepared BN powders exist in multiwall nanocages with dimensions from about 20 to 200 nm and have very good anti-oxidation properties at high temperatures.

Yang, Zeheng; Shi, Liang; Chen, Luyang; Gu, Yunle; Cai, Peijun; Zhao, Aiwu; Qian, Yitai

2005-03-01

250

Antioxidant properties and preliminary evaluation of phytochemical composition of different anatomical parts of amaranth.  

PubMed

Antioxidant properties of amaranth extracts isolated sequentially by acetone and methanol/water from defatted plant leaves, flowers, stems and seeds were assessed by ABTS(+•), DPPH(•), ORAC and total phenols content (TPC) assays. In addition, antioxidant properties of solid plant material were evaluated by the direct QUENCHER method using the same assays. Leaves and flowers of amaranth as well as their extracts possessed the highest antioxidant activities. Radical scavenging capacity in ABTS(+•) assay for leaves, flowers, stems and seeds evaluated by QUENCHER method were 144.24?±?2.41, 112.33?±?7.45, 19.05?±?1.13 and 21.82?±?1.06 ?mol trolox equivalents in 1 g of dry weight, respectively. On-line HPLC-DPPH(•) assay was used to determine the activity of separated compounds and it was observed that rutin was the main radical scavenger in amaranth extracts. Preliminary screening of extract composition was performed by UPLC/ESI-QTOF-MS and rutin, nicotiflorin, isoquercitrin, 4-hydroxybenzoic and p-coumaric acids were identified by measuring their accurate mass and retention time. PMID:23912804

Kraujalis, Paulius; Venskutonis, Petras R; Kraujalien?, Vaida; Pukalskas, Audrius

2013-09-01

251

Anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides)--an in vitro study.  

PubMed

This study was designed to determine the anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides) using lymphocytes as a model system. Chromium(VI) as potassium dichromate was used to induce oxidative damage. The production of free radicals by chromium and the ability of alcoholic leaf and fruit extracts of seabuckthorn to inhibit the oxidative damage induced by chromium was investigated. Addition of chromium (10 microg/ml) to the cells resulted in enhanced cytotoxicity, apoptosis, free radical production and decreased glutathione (reduced) levels. Chromium also caused a significant inhibition of lymphocyte proliferation induced by both lipopolysaccharide and concanavalin A. Alcoholic extracts of leaves and fruits of seabuckthorn at a concentration of 500 microg/ml were found to inhibit chromium-induced free radical production, apoptosis, DNA fragmentation and restored the anti-oxidant status to that of control cells. In addition, these extracts also were able to arrest the chromium-induced inhibition of lymphocyte proliferation. These observations suggest that the alcoholic extracts of leaves and fruits of seabuckthorn have marked cytoprotective properties, which could be attributed to the anti-oxidant activity. PMID:11849845

Geetha, S; Sai Ram, M; Singh, V; Ilavazhagan, G; Sawhney, R C

2002-03-01

252

Antioxidant properties of diorganoyl diselenides and ditellurides: modulation by organic aryl or naphthyl moiety.  

PubMed

Diorganoyl dichalcogenide compouds can have antioxidant activity in different in vitro and in vivo models. Here, we have compared the potential antioxidant activity of 1-dinaphthyl diselenide (1-NapSe)(2), 2-dinaphthyl diselenide (2-NapSe)(2), 1-dinaphthyl distelluride (1-NapTe)(2), 2-dinaphthyl ditelluride (2-NapTe)(2) with their well-studied analogs diphenyl diselenide ((PhSe)(2)) and diphenyl telluride ((PhTe)(2)). (PhSe)(2), (PhTe)(2), and naphthalene analogs-inhibited Fe(II)-induced lipid peroxidation, catalytically decomposed hydrogen peroxide and oxidized thiols, such as dithiothreitol (DTT), Cysteine (CYS), dimercaptopropionic acid (DMPS), and thiophenol (PhSH). (PhSe)(2) was the less potent of the tested compounds against Fe(II)-induced lipid peroxidation in brain homogenates and the change in the organic moiety from an aryl to naphthyl group increased considerably the antioxidant potency of diselenide compounds. However, the change from aryl to naphthyl had little effect on the thio-peroxidase-like activity of diorganoyl dichalcogenides. These results suggest that minor changes in the organic moiety of aromatic diselenide compounds can modify profoundly their capacity to inhibit iron-induced lipid peroxidation. The pharmacological properties of organochalcogens are thought to be linked to their capacity of modulating oxidative stress. Consequently, it becomes important to explore the toxicological properties of dinaphthyl diselenides and ditellurides. PMID:22983825

Ibrahim, Mohammad; Hassan, Waseem; Meinerz, Daiane Francine; Dos Santos, Matheus; V Klimaczewski, Claudia; M Deobald, Anna; Costa, Maricilia S; Nogueira, Cristina W; Barbosa, Nilda B V; Rocha, Joao B T

2012-09-16

253

Seismic characterization of fracture properties  

SciTech Connect

The purpose of this paper is to show that there is a relationship, both empirical and theoretical, between the measured seismic response, the mechanical stiffness (also referred to as specific stiffness) of fractures and their hydraulic conductivity. Laboratory measurements of the mechanical stiffness, hydraulic conductivity and seismic properties of natural fractures are summarized. A theoretical model for the amplitude and group time delay for compressional and shear waves transmitted across a single fracture is presented. Predictions based on this model are compared with laboratory measurements. Finally, the results for a single fracture are extended to multiple parallel fractures. 13 refs., 6 figs.

Myer, L.R.; Hopkins, D.; Cook, N.G.W. (Lawrence Berkeley Lab., CA (USA)); Pyrak-Nolte, L.J. (Purdue Univ., Lafayette, IN (USA). Dept. of Earth and Atmospheric Sciences)

1990-01-01

254

Characterization and antioxidant potential of Brazilian fruits from the Myrtaceae family.  

PubMed

The objective of this study is to evaluating the Brazilian biodiversity through physicochemical characterization and determination of antioxidant potential of three species from the Myrtaceae family, namely yellow guava (Psidium cattleyanum Sabine), guabiroba (Campomanesia xanthocarpa O. Berg), and uvaia ( Eugenia pyriformis Cambess). Guabiroba had the greater quantity of phenolic compounds (9033 mg chlorogenic acid/100 g) and vitamin C (30.58 mg/g) and showed the best TSS/TTA (total soluble solid/total titratable acid) ratio (45.12). For the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic) method, the guabiroba (507.49 ?M Trolox/g) presented the highest antioxidant potential; however, in the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, uvaia (170.26 g/g DPPH) and guabiroba (161.29 g/g DPPH) were not statistically different. The uvaia outranked the other fruits with respect to its high carotenoid (909.33 ?g/g) and vitamin A (37.83 ?g/g) contents, and the yellow guava, although showing a lower bioactive compound content and antioxidant activity, nevertheless presented much higher values than many traditionally consumed fruits. PMID:22397467

Pereira, Marina C; Steffens, Rosana S; Jablonski, André; Hertz, Plinho F; Rios, Alessandro de O; Vizzotto, Márcia; Flôres, Simone H

2012-03-19

255

Montmorillonite intercalated with glutathione for antioxidant delivery: synthesis, characterization, and bioavailability evaluation.  

PubMed

A most powerful antioxidant, glutathione (GSH), plays an important role in detoxification, immune response, and protection against reactive oxygen species. However, orally ingested GSH can be easily degradable to free amino acids by chemical and enzymatic hydrolysis, resulting in low bioavailability. The aim of this study was, therefore, to enhance GSH bioavailability by developing GSH-montmorillonite (MMT) hybrid system. It was also coated with polyvinylacetal diethylaminoacetate (AEA) for better stability. Both GSH-MMT and AEA-GSH-MMT hybrids were characterized by powder X-ray diffraction (PXRD), Fourier transformed infrared (FT-IR), and thermogravimetric analysis (TGA), indicating that GSH was successfully intercalated into the interlayer spaces of MMT. In vivo antioxidant activity assay revealed that AEA-GSH-MMT hybrid significantly increased antioxidant activity in the plasma after oral administration in mice. Pharmacokinetic study also indicated that AEA-GSH-MMT hybrid considerably increased the plasma concentration of GSH at 1h post-oral administration. Moreover, both the hybrid systems remarkably enhanced GSH delivery to the main target tissue, liver. All the results suggest that GSH-MMT hybrid systems have great potential to enhance bioavailability of oral GSH, providing new insight into their pharmaceutical application. PMID:22266539

Baek, Miri; Choy, Jin-Ho; Choi, Soo-Jin

2012-01-14

256

Antioxidative properties of hydroxycinnamic acid derivatives and a phenylpropanoid glycoside. A pulse radiolysis study  

NASA Astrophysics Data System (ADS)

Spectral and redox properties of the phenoxyl radicals from hydroxycinnamic acid derivatives and one selected component of phenylpropanoid glycosides, verbascoside, were studied using pulse radiolysis techniques. On the basis of the pH dependence of phenoxyl radical absorptions, the pKa values for deprotonation of sinapic acid radical and ferulic acid radical are 4.9 and 5.2. The rate constants of one electron oxidation of those antioxidants by azide radical and bromide radical ion were determined at pH 7. The redox potentials of those antioxidants were determined as 0.59-0.71 V vs NHE at pH 7 with reference standard 4-methoxyphenol and resorcinol.

Lin, W.; Navaratnam, S.; Yao, S.; Lin, N.

1998-10-01

257

Essential oil of the leaves of Eugenia uniflora L.: antioxidant and antimicrobial properties.  

PubMed

Essential oil (EO) of the leaves of Eugenia uniflora L. (Brazilian cherry tree) was evaluated for its antioxidant, antibacterial and antifungal properties. The acute toxicity of the EO administered by oral route was also evaluated in mice. The EO exhibited antioxidant activity in the DPPH, ABTS and FRAP assays and reduced lipid peroxidation in the kidney of mice. The EO also showed antimicrobial activity against two important pathogenic bacteria, Staphylococcus aureus and Listeria monocytogenes, and against two fungi of the Candida species, C. lipolytica and C. guilliermondii. Acute administration of the EO by the oral route did not cause lethality or toxicological effects in mice. These findings suggest that the EO of the leaves of E. uniflora may have the potential for use in the pharmaceutical industry. PMID:22583648

Victoria, Francine Novack; Lenardão, Eder João; Savegnago, Lucielli; Perin, Gelson; Jacob, Raquel Guimarães; Alves, Diego; da Silva, Wladimir Padilha; da Motta, Amanda de Souza; Nascente, Patricia da Silva

2012-05-11

258

Tronchuda cabbage ( Brassica oleracea L. var. costata DC) seeds: Phytochemical characterization and antioxidant potential  

Microsoft Academic Search

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS\\/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-4?-(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,2?-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also

Federico Ferreres; Carla Sousa; Patrícia Valentão; Rosa M. Seabra; José A. Pereira; Paula B. Andrade

2007-01-01

259

In vitro antioxidant and cytotoxic properties of ethanol extract of Alpinia oxyphylla fruits.  

PubMed

Abstract Context. Alpinia oxyphylla Miquel (Zingiberaceae) is a traditional Chinese herbal medicine widely used for the treatment of intestinal disorders, urosis and diuresis. However, information about antioxidant and cytotoxic properties of its fruits remains to be elucidated. Objective: The ethanol crude extract (CE) and its fractions [petroleum ether fraction (PF), ethyl acetate fraction (EF), n-butanol fraction (BF) and water fraction (WF) extracted by petroleum ether, ethyl acetate, n-butanol and water, respectively] of A. oxyphylla fruits were investigated for their antioxidant activity and cytotoxicity. Materials and methods: The total phenolic content (TPC) and antioxidant activity of the extracts were determined by Folin-Ciocalteu reagent, 1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), Trolox equivalent antioxidant capacity and reducing power assay. Cytotoxicity of the extracts (0-200??g/mL) was tested on six human cancer cell lines (breast cancer cell line, cervix carcinoma cell line, lung adenocarcinoma cell line, liver carcinoma cell line, gastric cancer cell line and colon cancer cell line) using the sulforhodamine B assay. Results: The TPC of extracts varied from 8.2 to 20.3?mg gallic acid equivalents/g dry weight. DPPH radical scavenging effect of extracts decreased in the order of EF?>?BF?>?CE?>?PF?>?WF, with IC50 values ranging from 74.7 to 680.8??g/mL. 2,2-azo-bis(3-Ethylbenzothiazoline-6-sulfoic acid) diammonium salt scavenging activity ranged from 0.118 to 0.236?mmol Trolox equivalence/mg extract. The extracts exhibited concentration-dependent reducing power, and EF showed the highest reducing ability. A satisfactory correlation (R(2)?>?0.826) between TPC and antioxidant activity was observed. In addition, EF, PF and CE exhibited potent anticancer effects on six cancer cell lines with IC50 values ranging from 40.1 to 166.3??g/mL. Discussion and conclusion: The ethanol extract of A. oxyphylla fruit, especially the EF, was found to possess potent antioxidant and anticancer activities, and thus a great potential for the application in food and drug products. PMID:23841520

Wang, Cheng-Zhong; Yuan, Hui-Hui; Bao, Xiao-Li; Lan, Min-Bo

2013-07-10

260

Comparative study of antioxidant properties of wild growing and cultivated Allium species.  

PubMed

Allium species are cultivated for the edible bulb, which is used mainly as flavoring in foods. Besides that, they could prevent tumor promotion and some processes that are associated with free radicals, such as cardiovascular diseases and aging. Therefore, different Allium species, both cultivated (Allium nutans L., A. fistulosum L., A. vineale L., A. pskemense B. Fedtsch, A. schoenoprasum L., A. cepa L. and A. sativum L.) and wild (A. flavum L., A. sphaerocephalum L., A. atroviolaceum Boiss, A. vineale L., A. ursinum L., A. scorodoprasum L., A. roseum L. and A. subhirsutum L.), were investigated in order to evaluate the antioxidant properties of their bulbs. This study reports on the results obtained for the bulb antioxidant enzyme activities (superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase), the quantities of non-enzymatic plant antioxidants (reduced glutathione and total flavonoids), the contents of soluble proteins, vitamin C, carotenoids, chlorophylls a and b, as well as for the quantities of malonyldialdehyde and .OH and O2.- radicals. PMID:17726730

Stajner, D; Igi?, R; Popovi?, B M; Malenci?, Dj

2008-01-01

261

Cultivar variations in antioxidant and antihyperlipidemic properties of pomelo pulp (Citrus grandis [L.] Osbeck) in Thailand.  

PubMed

Pomelo (Citrus grandis L. Osbeck) is a native fruit of great economic importance in Southeast Asia. To provide experimental evidence for the antioxidant and antihyperlipidemic properties of pomelo, 6 cultivars, including Kao-Yai (KY), Thong-dee (TD), Kao-Tangkwa (KT), Kao-Numpueng (KN), Ta-Koi (TK), and Tubtim Siam (TS) were evaluated. KY had the highest phenolic content, and the strongest 1,1-diphenyl-2-pireyhydrazyl radical scavenging capacity and hydroxyl radical scavenging activity. From the high-performance liquid chromatography analysis, naringin and naringenin were the major flavonoids in the KT and TK cultivars. Six pomelo cultivars had antihyperlipidemic activities including the inhibition of pancreatic lipase and cholesterol esterase, as well as cholesterol micelle formation and bile acid binding. Hierarchical clustering analysis showed that the 6 cultivars were separated into 2 classifications. In addition, the total phenolics of the pomelo cultivars were significantly correlated with ferric reducing antioxidant power and Trolox equivalent antioxidant capacity. The results suggest that pomelo provides significant health benefits and may be used for developing functional foods. PMID:23561168

Mäkynen, Kittana; Jitsaardkul, Sritanaporn; Tachasamran, Pansiree; Sakai, Nathaporn; Puranachoti, Supitcha; Nirojsinlapachai, Natthapat; Chattapat, Vipaporn; Caengprasath, Natarin; Ngamukote, Sathaporn; Adisakwattana, Sirichai

2013-02-16

262

The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin, and allyl disulfide.  

PubMed

Garlic and garlic extracts, through their antioxidant activities, have been reported to provide protection against free radical damage in the body. This study investigated antioxidant properties of garlic compounds representing the four main chemical classes, alliin, allyl cysteine, allyl disulfide, and allicin, prepared by chemical synthesis or purification. Alliin scavenged superoxide, while allyl cysteine and allyl disulfide did not react with superoxide. Allicin suppressed the formation of superoxide by the xanthine/xanthine oxidase system, probably via a thiol exchange mechanism. Alliin, allyl cysteine, and allyl disulfide all scavenged hydroxyl radicals; the rate constants calculated based on deoxyribose competitive assay were 1.4-1.7 x 10(10), 2.1-2.2 x 10(9), and 0.7-1.5 x 10(10) M (1) second(1), respectively. Contrary to previous reports, allicin did not exhibit hydroxyl radical scavenging activity in this study. Alliin, allicin, and allyl cysteine did not prevent induced microsomal lipid peroxidation, but both alliin and allyl cysteine were hydroxyl scavengers, and allyl disulfide was a lipid peroxidation terminator. In summary, our findings indicated that allyl disulfide, alliin, allicin, and allyl cysteine exhibit different patterns of antioxidant activities as protective compounds against free radical damage. PMID:16822206

Chung, Lip Yong

2006-01-01

263

Betalain, Acid ascorbic, phenolic contents and antioxidant properties of purple, red, yellow and white cactus pears.  

PubMed

Commercialization of cactus pears based on their antioxidant properties can generate competitive advantages, and these can turn into business opportunities and the development of new products and a high-value ingredient for the food industry. This work evaluated the antioxidant activities (1,1-diphenyl-2-picrylhydrazyl radical-scavenging, protection against oxidation of a ?-carotene-linoleic acid emulsion, and iron (II) chelation), the content of total phenolic compounds, ascorbic acid, betacyanin, betaxanthin and the stability of betacyanin pigments in presence of Cu (II)-dependent hydroxyl radicals (OH•), in 18 cultivars of purple, red, yellow and white cactus pear from six Mexican states. Our results indicated that the antiradical activities from yellow and white cactus pear cultivars were not significantly different (p < 0.05) and were lower than the average antiradical activities in red and purple cultivars. The red cactus pear from the state of Zacatecas showed the highest antioxidant activity. The free radical scavenging activity for red cactus pears was significantly correlated (p < 0.05) to the concentration of total phenolic compounds (R(2) = 0.90) and ascorbic acid (R(2) = 0.86). All 18 cultivars of cactus pears studied showed significant chelating activity of ferrous ions. The red and purple cactus pears showed a great stability when exposed to OH•. PMID:22072899

Sumaya-Martínez, María Teresa; Cruz-Jaime, Sandra; Madrigal-Santillán, Eduardo; García-Paredes, Juan Diego; Cariño-Cortés, Raquel; Cruz-Cansino, Nelly; Valadez-Vega, Carmen; Martinez-Cardenas, Leonardo; Alanís-García, Ernesto

2011-09-28

264

ATP-regulation of antioxidant properties and phenolics in litchi fruit during browning and pathogen infection process  

Microsoft Academic Search

The impact of energy level on antioxidant properties in relation to pericarp browning and loss of disease resistance of litchi fruit was investigated. Litchi fruits were vacuum-infiltrated with distiled water (control), 1mM adenosine triphosphate (ATP) and 0.5mM 2,4-dinitrophenol (DNP) under 75kPa for 3min before being inoculated with Peronophythora litchi or not. ATP-treated fruits presented higher activities of antioxidant enzymes, including

Chun Yi; Yueming Jiang; John Shi; Hongxia Qu; Sophia Xue; Xuewu Duan; Jingyu Shi; Nagendra K. Prasad

2010-01-01

265

Antioxidative properties of aqueous and aroma extracts of squid miso prepared with Aspergillus oryzae-inoculated koji  

Microsoft Academic Search

To evaluate the antioxidative properties of a newly developed fermented paste (squid miso) prepared from squid mantle flesh with Aspergillus oryzae-inoculated koji, we used in vitro models, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, nitric oxide (NO) and carbon-centered radical-scavenging activity (RSA), reducing power ability (RPA), and linoleic acid oxidation. The antioxidant activity of volatile compounds, which were extracted from squid miso by

Anupam Giri; Kazufumi Osako; Akira Okamoto; Emiko Okazaki; Toshiaki Ohshima

2011-01-01

266

Antioxidant Properties of Fruits and Vegetables Shots and Juices: An Electron Paramagnetic Resonance Study  

Microsoft Academic Search

The antioxidant activity of some commercially available fruit and vegetable juices was evaluated with regard to their radical\\u000a scavenging activity against the stable free radical 4-hydroxy-2,2,6,6-tetramethyl-l-piperidinyloxy (TEMPOL) monitored by electron\\u000a paramagnetic resonance (EPR) spectroscopy. TEMPOL is a stable nitroxide free radical characterized by a well-defined EPR spectrum\\u000a consisting of three peaks. The integral intensity of the EPR spectra of TEMPOL

Evaggelia D. Tzika; Vassiliki Papadimitriou; Theodore G. Sotiroudis; Aristotelis Xenakis

2008-01-01

267

Physicochemical Characterization and Antioxidant Activity of Essential Oils of Guggul (Commiphora wightii) Collected from Madhya Pradesh  

PubMed Central

The present study reports physicochemical characterization and antioxidant activity of essential oils extracted from guggul (Commiphora wightii) exudates collected from different places in Madhya Pradesh, India. The guggul exudates were hydrodistilled for 3-4 h in Clevenger apparatus. The oil obtained was dried over anhydrous Na2SO4 and stored at 4° until testing. Before extraction of oils from the exudates, their % moisture and tristimulus values of the colors namely L (white-black), a (green-red) and b (blue-yellow) were determined. Physicochemical characterization of the extracted oils was carried out to determine their solubility, yield%, acid value (mg/KOH/g), saponification value (mg/KOH/g), ester value, iodine value (g/g), peroxide value (mEq/kg) and Fourier transformed infrared analyses. The storage-effect on the % moisture and tristimulus values of the colors of guggul exudates as also the % oil yield and physicochemical parameters of the essential oils extracted from them, were studied using three different packaging materials viz., local plastic, low density polyethylene (200 G) and high density polyethylene (200 G). The antioxidant potential of extracted oils was evaluated by free radical scavenging activity using 1,1-diphenyl-2-picryl hydrazyl assay.

Siddiqui, M. Z.; Thomas, M.; Prasad, N.

2013-01-01

268

Structural identification and antioxidant properties of major anthocyanin extracted from Omija (Schizandra chinensis) fruit.  

PubMed

Omija (Schizandra chinensis) is used as an ingredient in traditional medicine in East Asia. It is consumed as tea and wine and display pinkish-red color and beneficial physiological activity. However, the origin of Omija's unique color and bioactivity has not been studied extensively and its application is very limited. Thus, it was required to determine the chemical structure of major phenolic compounds of Omija fruit and evaluate their antioxidant activity. The colorants extracted from a domestic Omija cultivar were concentrated by a Sep-pak(R) Plus C(18) cartridge. A major high-performance liquid chromatography (HPLC) peak of anthocyan represented 94.1% of total absorbable compounds at 520 nm, which was further identified by LC-ESI-MS. The mass-to-charge ratio (m/z) of the major anthocyan was determined to be 727. Highly pure anthocyan fraction with a semipreparative HPLC was acid-hydrolyzed, and the sugar moieties linked to anthocyan (cyanidin) were characterized by thin layer chromatography (TLC) and high-performance anion exchange chromatography (HPAEC) analyses. The linkage patterns of sugars and core cyanidin structure were determined by (1)H- and (13)C-NMR analyses. Antioxidant activity of the extract and the purified anthocyanin was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) methods. As a result, the structure of the purified colorant was identified as Cya-3-O-xylrut. At the same molar level of the samples tested, the purified Cya-3-O-xylrut (31.2% and 39.2%) had substantially greater antioxidant activity than l-ascorbic acid (17.1% and 10.1%) from DPPH and ABTS methods, respectively. In this study, Omija colorant mostly consisted of Cya-3-O-xylrut explained 86% (DPPH) and 98% (ABTS) of total antioxidant activity derived from water extract from Omija. PMID:19323727

Kim, S-H; Joo, M H; Yoo, S-H

2009-03-01

269

Synthesis, characterization and optical properties of nanoparticles  

Microsoft Academic Search

ZnO, Si, silica, Ge, Ga oxide, W oxide and Mo oxide nanoparticles have been synthesized and characterized, and their optical properties have been investigated. These particles were synthesized by a Laser Vaporization and Controlled Condensation (LVCC) technique in a modified diffusion cloud chamber. The particles deposited on smooth substrates reveal highly organized web-like structures with uniform micrometer size pores. The

Shoutian Li

1998-01-01

270

Functional, antioxidant and film-forming properties of tuna-skin gelatin with a brown algae extract.  

PubMed

Characteristics and functional properties of gelatin from skin of Atlantic Bluefin tuna (Thunnus thynnus) were investigated. The gelatin was extracted by an acid-swelling process in the presence of different concentrations of commercial pepsin, followed by subsequent heating. The extraction yield was higher when increasing concentrations of pepsin were used during the swelling process. Emulsion activity index, foam formation ability and foam stability of gelatin increased with the increase of gelatin concentration. Antioxidant properties (ferric-reducing ability and DPPH-radical-scavenging capacity) of gelatin-based edible films containing aqueous or methanolic extracts of brown algae (Cystoseira barbata) were also assessed. For comparative purposes, tuna-skin gelatin edible film with BHA was studied. Antioxidant properties of the films were increased significantly when natural extracts were added. Extracts of brown algae could be useful additives to obtain edible films from tuna-skin gelatin with interesting functional and antioxidant properties. PMID:22721871

Haddar, Anissa; Sellimi, Sabrine; Ghannouchi, Raouia; Alvarez, Oscar Martinez; Nasri, Moncef; Bougatef, Ali

2012-06-18

271

Investigation of the antioxidant properties of hyperjovinol A through its Cu(II) coordination ability.  

PubMed

Hyperjovinol A (2-methyl-1-(2,4,6-trihydroxy-3-(3-hydroxy-3,7-dimethyloct-6-enyl)phen yl)propan-1-one) is an acylated phloroglucinol isolated from Hypericum Jovis and exhibiting antioxidant properties comparable with those of the most common antioxidant drugs. The study models the compound's antioxidant ability through its ability to coordinate a Cu(2+) ion and reduce it to Cu(+). Complexes with a Cu(2+) ion were calculated for all the low energy and for representative high energy conformers of hyperjovinol A, placing the ion in turn near each of the electron-rich binding sites. The most stable complexes are those in which Cu(2+) binds simultaneously to the O of the OH in the geranyl-type chain (R') and the C?C double bond at the end of R', or to the O of a phenol OH and the O of the OH in R'. The most stable complexes in which Cu(2+) binds only to one site are those in which it binds to the C?C double bond at the end of R' or to the sp(2) O of the COCH(CH3)2 acyl group. Cu(2+) is reduced to Cu(+) in all complexes. Comparisons with corresponding complexes of other molecular structures in which one or more of the structural features of hyperjovinol A are modified attempt to elucidate the role, for the antioxidant ability, of relevant features of hyperjovinol A, like the presence and position of the OH or the C?C double bond in R'. Calculations at the DFT/B3LYP/6-31+G(d,p) level were performed for all the structures considered. Calculations utilizing the LANL2DZ pseudopotential for the Cu(2+) ion were also performed for hyperjovinol A. PMID:23212237

Mammino, Liliana

2012-12-05

272

Antioxidant activity of indole-based melatonin analogues in erythrocytes and their voltammetric characterization.  

PubMed

Melatonin (MLT) is a strong free-radical scavenger, which protects the body from the effects of oxidants. In recent years, MLT have been described resulting in much attention in the development of synthetic compounds possessing. As a part of our ongoing study a series of indole-based MLT analogue hydrazide/hydrazone derivatives were synthesized, characterized and in vitro antioxidant activity was investigated by evaluating their reducing effect against oxidation of a redox sensitive fluorescent probe. Membrane stabilizing effect of all compounds was also investigated by lactate dehydrogenase leakage assay. Furthermore voltammetric methods have been applied to the synthesized compounds to characterize oxidation potentials to get insight into their metabolism owing to the oxidation mechanisms taking place at the electrode and in the body share similar principles. PMID:22994658

Suzen, Sibel; Tekiner-Gulbas, Betul; Shirinzadeh, Hanif; Uslu, Duysal; Gurer-Orhan, Hande; Gumustas, Mehmet; Ozkan, Sibel Aysil

2012-09-20

273

Antioxidative and functional properties of protein hydrolysate from defatted skipjack (Katsuwonous pelamis) roe.  

PubMed

Antioxidative and functional properties of protein hydrolysate from defatted skipjack (Katsuwonous pelamis) roe, hydrolysed by Alcalase 2.4 L (RPH) with different degrees of hydrolysis (DH) at various concentrations were examined. As DH increased, the reduction of DPPH, ABTS radicals scavenging activities and reducing power were noticeable (p<0.05). The increases in metal chelating activity and superoxide scavenging activity were attained with increasing DH (p<0.05). However, chelating activity gradually decreased at DH above 30%. All activities except superoxide anion radical scavenging activity increased as the concentration of hydrolysate increased (p<0.05). Hydrolysis using Alcalase could increase protein solubility to above 80% over a wide pH range (2-10). The highest emulsion ability index (EAI) and foam stability (FS) of hydrolysates were observed at low DH (5%) (p<0.05). Concentrations of hydrolysates determined interfacial properties differently, depending on DH. The molecular weight distribution of RPH with 5%DH (RPH5) was determined using Sephadex G-75 column. Two major peaks with the molecular weight of 57.8 and 5.5kDa were obtained. Fraction with MW of 5.5 had the strongest metal chelating activity and ABTS radical scavenging activity. The results reveal that protein hydrolysates from defatted skipjack roe could be used as food additives possessing both antioxidant activity and functional properties. PMID:22980906

Intarasirisawat, Rossawan; Benjakul, Soottawat; Visessanguan, Wonnop; Wu, Jianping

2012-06-29

274

Development of naturally activated edible films with antioxidant properties prepared from red seaweed Porphyra columbina biopolymers.  

PubMed

The aim of this work was to study the physicochemical and antioxidant properties of phycobiliproteins-phycocolloids-based films, obtained from mixtures of two aqueous fractions extracted from Porphyra columbina red seaweed, one enriched in phycocolloids (PcF) and the other in phycobiliproteins (PF). Films with different ratios of PF:PcF (0, 25, 50, 75, 100% [w/w]) and without plasticizer addition were prepared by casting. PcF films had excellent mechanical properties (tensile strength ?50MPa, elongation at break ?3% and an elastic modulus ?17.5MPa). The addition of PF to formulations exerted a plasticizing effect on the PcF matrix, which was manifested in moisture content, water solubility and mechanical properties of the resulting films but not in its water vapour permeability. The antioxidant capacity (TEAC) of the PcF films was significantly increased by the addition of PF and a direct relationship between TEAC and the total phenolic compounds (r(2)=0.9998) and R-phycoerythrin (r(2)=0.9942) was observed. PMID:24176306

Cian, Raúl E; Salgado, Pablo R; Drago, Silvina R; González, Rolando J; Mauri, Adriana N

2013-09-14

275

Physicochemical and antioxidant properties of cornelian cherry fruits ( Cornus mas L.) grown in Turkey  

Microsoft Academic Search

Cornus mas L. is a naturally growing dogwood species in Anatolia. In present study, physical, chemical and antioxidant properties of cornelian cherry fruits were studied. The fruit weight was in the range of 0.39–1.03g, fruit length 14.24–22.20mm, fruit width 9.59–13.21mm, flesh\\/seed ratio 1.34 to 6.72. Hunter L values of the samples ranged between 10.82 and 19.69, and a value was

Serpil Tural; Ilkay Koca

2008-01-01

276

Comparative study on the chemical composition, antioxidant properties and hypoglycaemic activities of two Capsicum annuum L. cultivars (Acuminatum small and Cerasiferum).  

PubMed

The present study aimed to evaluate for the first time the phenols, flavonoids, carotenoids, capsaicin and dihydrocapsaicin content and the antioxidant and hypoglycemic properties of Capsicum annuum var. acuminatum small and C. annuum var. cerasiferum air-dried fruits. The ethanol extract of C. annuum var. acuminatum small, characterized by the major content of total poliphenols, flavonoids, carotenoids and capsaicinoids, showed the highest radical scavenging activity (IC(50) of 152.9 ?g/ml). On the contrary, C. annuum var. cerasiferum showed a significant antioxidant activity evaluated by the ?-carotene bleaching test (IC(50) of 3.1 ?g/ml). The lipophilic fraction of both C. annuum var. acuminatum and C. annuum var. cerasiferum exhibited an interesting and selective inhibitory activity against ?-amylase (IC(50) of 6.9 and 20.1 ?g/ml, respectively). PMID:21792679

Tundis, Rosa; Loizzo, Monica R; Menichini, Federica; Bonesi, Marco; Conforti, Filomena; Statti, Giancarlo; De Luca, Damiano; de Cindio, Bruno; Menichini, Francesco

2011-09-01

277

Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants  

Microsoft Academic Search

In order to find out new sources of safe and inexpensive antioxidants, the antioxidant capacities of 45 selected medicinal plants were evaluated using ferric reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) assays, respectively, and the total phenolic contents of these plants were measured by the Folin–Ciocalteu method. Most of these plants were analyzed for the first time

Hua-Bin Li; Chi-Chun Wong; Ka-Wing Cheng; Feng Chen

2008-01-01

278

Thiocyanate: a potentially useful therapeutic agent with host defense and antioxidant properties.  

PubMed

Thiocyanate (SCN) functions in host defense as part of the secreted lactoperoxidase (LPO) microbicidal pathway. SCN is the preferred substrate for LPO-driven catalytic reduction of hydrogen peroxide (H(2)O(2)) forming hypothiocyanous acid (HOSCN). HOSCN is selectively generated by many peroxidase enzymes that can utilize SCN including: eosinophil peroxidase (EPO), gastric peroxidase (GPO), myeloperoxidase (MPO), salivary peroxidase (SPO), and thyroid peroxidase (TPO). These enzymes generate HOSCN through a two-electron halogenation reaction. HOSCN is a potent microbicidal agent that kills or nullifies invading pathogens but is better tolerated by host tissue. Some controversy exists as to whether physiologic levels of HOSCN are non-toxic to host tissue, but the disagreement appears to be based on results of enzymatic generation (yielding moderate steady-state exposure) versus direct high level acute exposure in mammalian cell lines. This apparent duality is also true of other endogenous oxidants such as hydrogen peroxide and relates to the difference between physiologically relevant oxidant production versus supra-physiologic bolus dosing approaches. SCN has antioxidant properties that include the ability to protect cells against oxidizing agents such as hypochlorous acid (HOCl) and repair protein chloramines. SCN is an important endogenous molecule that has the potential to interact in complex and elegant ways with its host environment and foreign organisms. SCN's diverse properties as both host defense and antioxidant agent make it a potentially useful therapeutic. PMID:22968041

Chandler, Joshua D; Day, Brian J

2012-08-08

279

Phenolic, polysaccharidic, and lipidic fractions of mushrooms from northeastern Portugal: chemical compounds with antioxidant properties.  

PubMed

Mushrooms do not constitute a significant portion of the human diet, but their consumption continues to increase due to their functional benefits and presence of bioactive compounds. Some of those compounds can be found in the phenolic, polysaccharidic, and lipidic fractions of edible and inedible species. Herein, those fractions of five wild mushrooms (Coprinopsis atramentaria, Lactarius bertillonii, Lactarius vellereus, Rhodotus palmatus, and Xerocomus chrysenteron) from northeastern Portugal were studied for their chemical composition and antioxidant properties. Protocatechuic, p-hydroxybenzoic, p-coumaric, and cinnamic acids were found in the phenolic fraction; rhamnose, xylose, fucose, arabinose, fructose, glucose, manose, mannitol, sucrose, maltose, and trehalose were quantified in polysaccharidic fraction; and linoleic and stearic (only in Lactarius sp.) acids, and ?- and ?-tocopherols were the main compounds in the lipidic fraction. C. atramentaria and X. chrysenteron phenolic fractions gave the highest free radical scavenging activity, reducing properties, and lipid peroxidation inhibition in brain homogenates, which is in agreement with its highest content in total phenolics. Furthermore, among the polysaccharidic fractions C. atramentaria also gave the highest antioxidant activity, which is in agreement with its highest total polysaccharides content and sugars obtained after hydrolysis. PMID:22515547

Heleno, Sandrina A; Barros, Lillian; Martins, Anabela; Queiroz, Maria João R P; Santos-Buelga, Celestino; Ferreira, Isabel C F R

2012-04-27

280

Synthesis and antioxidant properties of substituted 3-benzylidene-7-alkoxychroman-4-ones  

Microsoft Academic Search

A series of 3-benzylidene-7-alkoxychroman-4-one derivatives were synthesized and evaluated for their antioxidant activities. The antioxidant activity was assessed using three methods, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and thiobarbituric acid reactive substances (TBARS) assays. 3-Benzylidene-7-alkoxychroman-4-one derivatives bearing catecholic group on benzylidene moiety exhibited excellent antioxidant activity. Compounds having catechol moiety exhibited potent antioxidant activities in all

Alireza Foroumadi; Alireza Samzadeh-Kermani; Saeed Emami; Gholamreza Dehghan; Maedeh Sorkhi; Fatemeh Arabsorkhi; Mahmoud Reza Heidari; Mohammad Abdollahi; Abbas Shafiee

2007-01-01

281

Propolis as potential cosmeceutical sunscreen agent for its combined photoprotective and antioxidant properties.  

PubMed

Propolis, bee glue, and its main polyphenolic components show high antioxidant activity as found measuring their inhibitory action on lipid peroxidation of linoleic acid (LA) in sodium dodecyl sulfate (SDS) micelles. Furthermore, these substances evidence effectiveness as broad spectrum UVB and UVA photoprotection sunscreens, as it results by measurements of sun protection factor (SPF), the universal indicator related primarily to UVB radiations, and of the two parameters giving an indication of the UVA absorbance properties, i.e. UVA/UVB ratio and critical wavelength. The combination of these characteristics moves up propolis and its main polyphenolic components to the class of cosmeceuticals, as possible active ingredient of sunscreen commercial formulations for their protective and preventive properties. PMID:21134430

Gregoris, Elena; Fabris, Sabrina; Bertelle, Mariangela; Grassato, Luigi; Stevanato, Roberto

2010-12-04

282

CHARACTERIZATION OF PHENOLIC COMPOUNDS IN STRAWBERRY FRUITS BY RP-HPLC-DAD AND INVESTIGATION OF THEIR ANTIOXIDANT CAPACITY  

Microsoft Academic Search

This research was undertaken to evaluate the phenolic compositions and antioxidant capacities of strawberry cultivars (Camarosa, Seyhun, and Osmanli) grown in Turkey. High performance liquid chromatography coupled with diode array and coulometric array detectors were used to characterize and quantify organic acids and phenolic compounds. Four organic acids (citric, malic, shikimic, and ascorbic acids) were determined. The major organic acid

Hasim Kelebek; Serkan Selli

2011-01-01

283

Synthesis, characterization, thermal and antioxidant studies of potassium dihydrobisphenothiazinyl borate and its transition metal complexes.  

PubMed

The bidentate borate anion H(2)B(ptz)(2)(-) and its transition metal complexes have been synthesized and characterized by elemental analyses, magnetic susceptibility, electronic, IR, (1)H and (13)C NMR data. The molar conductance of 10(-3) M solution for all the complexes supports their non-ionic nature. The TGA profile of borate anion shows a single stage unlike that of two stage decomposition plot of the metal complexes. On the basis of spectroscopic studies the geometry of all the complexes have been proposed to be distorted-tetrahedral. The in vitro antioxidant and lipid oxidation inhibition of the ligand and its complexes have also been studied. The Cu[B(ptz)(2)](2) complex was found to be most effective in all the studies. PMID:22885087

Nami, Shahab A A; Alam, Mahboob; Husain, Ahmad; Parveen, Mehtab

2012-07-20

284

Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials.  

PubMed

Propolis samples, collected from different geographical locations in Iraq (Baghdad, Dahuk, Mosul and Salah ad-Din), were analyzed and assessed for their anti-oxidant activity. Concentrations of phenolic compounds (flavonoids, phenolic acids and their esters) in propolis were estimated using high performance liquid chromatography coupled to electrospray mass spectrometry. Thirty-eight different compounds were identified and 33 of them were polyphenols. Other compounds were tentatively identified as clerodane diterpenoids, and one was considered unknown. Semi-quantitative measurements showed that phenolic acids and their esters were the predominant constituents in propolis extracts, followed by flavones and flavonols, and then flavanones and dihydroflavonols. Propolis samples were further spectrophotometrically characterized using the Folin-Ciocalteu reagent for the determination of total phenolic compounds. The free radical scavenging activities of propolis samples were also evaluated by using the 2,2-diphenyl-1-picrylhydrazyl assay. The results revealed that propolis extracts exhibited strong free radical scavenging activity. PMID:21723909

Sulaiman, Ghassan M; Al Sammarrae, Khulood W; Ad'hiah, Ali H; Zucchetti, Massimo; Frapolli, Roberta; Bello, Ezia; Erba, Eugenio; D'Incalci, Maurizio; Bagnati, Renzo

2011-06-24

285

Effects of oral dosage form and storage period on the antioxidant properties of four species used in traditional herbal medicine.  

PubMed

Herbal infusions and decoctions in water are some of the most commonly consumed beverages in the world. Although water is not a good solvent for many of the active components in herbs, liquid preparations are rich in several bioactive compounds. Most of them have powerful antioxidant activity and have been related to medicinal herbs' properties. Herein, decoctions and infusions in water of lemon-verbena (Aloysia citrodora) aerial parts and leaves, fennel (Foeniculum vulgare), pennyroyal (Mentha pulegium) and spearmint (Mentha spicata) aerial parts with different periods of storage (0, 30, 60 and 120 days), were prepared. The effects of the method of preparation and storage period on their antioxidant properties were analysed. For all the analysed species, infusions gave better results than the corresponding decoctions. Spearmint infusions showed the highest antioxidant properties, at all the storage periods, probably due to the highest levels and synergy between phenolics, flavonoids and ascorbic acid found in this sample. Linear discriminant analysis confirmed that the length of storage period has a significant influence on the antioxidant activity and antioxidant content. Flavonoids and reducing sugars proved to be the parameters that most highly contributed to cluster individual groups according to different periods of storage. PMID:20740475

Guimarães, Rafaela; Barreira, João C M; Barros, Lillian; Carvalho, Ana Maria; Ferreira, Isabel C F R

2010-08-24

286

[Antioxidant properties of remaxol, reamberin, and ademetionine in patients with drug-induced liver injury on the background of antituberculous therapy].  

PubMed

The antioxidant properties of remaxol, reamberin and ademetionine have been studied in comparison to 5 % glucose solution in a group of 120 patients with drug-induced liver injury. It is established that the inclusion of this drugs in the composition of complex therapy contributed to restoration of the antioxidant potential of the cells, which was manifested by increased activity of glutathione peroxidase and superoxide dismutase and integral indices of antioxidant protection (total antioxidant capacity and total antioxidant status) with a relative stabilization of the level of glutathione-S-transferase. Maximum pharmacotherapeutic effect with respect to all of the studiea indices has been achieved by the use of remaxol. PMID:23762990

Sukhanov, D S

2013-01-01

287

Curcumin Loaded Poly(?-Caprolactone) Nanofibers: Diabetic Wound Dressing with Antioxidant and Anti-inflammatory Properties  

PubMed Central

1. Curcumin is a naturally occurring poly-phenolic compound with a broad range of favorable biological functions including anti-cancer, anti-oxidant and anti-inflammatory activities. The low bioavailability and in vivo stability of curcumin require the development of suitable carrier vehicles to deliver the molecule in a sustained manner at therapeutic levels. 2. We investigated the feasibility and potential of poly(caprolactone) (PCL) nanofibers as a delivery vehicle for curcumin for wound healing applications. By optimizing the electrospinning parameters, bead-free curcumin loaded PCL nanofibers were developed. 3. The fibers showed sustained release of curcumin for 72 h and could be made to deliver a dosage much lower than the reported cytotoxic concentration while remaining bioactive. Human foreskin fibroblast cells (HFF-1) showed more than 70% viability on curcumin loaded nanofibers. The antioxidant activity of curcumin loaded nanofibers was demonstrated using an ORAC assay and by the ability of the fibers to maintain the viability of HFF-1 cells on the fibers under a condition of oxidative stress. The curcumin loaded nanofibers also reduced inflammatory induction as evidenced by low levels of IL-6 release from mouse monocyte-macrophages seeded on the fibers following stimulation by E.coli-derived lipopolysaccharide (LPS). The in vivo wound healing capability of the curcumin loaded PCL nanofibers was demonstrated by an increased rate of wound closure in a streptozotocin (STZ) induced diabetic mice model. 4. These results demonstrate that curcumin loaded PCL nanofiber matrix is bioactive and has potential as a wound dressing with antioxidant and anti-inflammatory properties.

Merrell, Jonathan G.; McLaughlin, Shaun W.; Tie, Lu; Laurencin, Cato T.; Chen, Alex F.; Nair, Lakshmi S.

2009-01-01

288

Anti-Iipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México.  

PubMed

We report the results of in vitro anti-lipase and antioxidant assays using crude ethanolic extracts from 30 plants grown in Oaxaca, México. Anti-lipase tests were performed by using porcine pancreatic lipase (PPL) [EC 3.1.1.3] from Affymetrix/USB. The extracts of Solanum erianthum, Salvia microphylla, Brungmansia suaveolens and Cuphea aequipetala showed up to 60% PPL inhibition. The effect of these extracts on the kinetic parameters of PPL (Km= 0.36 mM, and Vmax=0.085 mM min -1) revealed that the alcoholic preparations of S. erianthum and C. aequipetala engendered a non-competitive inhibition (Vmax=0.055 mM min -1; Vmax= 0.053 mM min -1), whereas those of S. microphylla and B. suaveolens produced a mixed inhibition (Km= 0.567 mM, Vmax=0.051 mM min _1; Km=0.643 mM, Vmax= 0.042 mM min ¹). In addition to these findings, seven extracts from different plants were able to inhibit PPL in the range of 30-50%. Antioxidant tests against 2,2-Diphenyl-1-picryl hydrazyl (DPPH) confirmed that Arctostaphylos pungens, Gnaphalium roseum, Crotalaria pumila, Cuphea aequipetala, Rhus chondroloma, and Satureja laevigata possess relevant antioxidant activity (IC(5)0=50-80 ?g mL¹). The general composition of the most effective ethanolic extracts was obtained in order to confirm their known chemistry reported by previous works. Comprehensive chemical analysis of the ethanolic extracts and their poisoning effects suggests that S. microphylla, C. aequipetala and A. pungens could be considered as the best sources with both desired properties. PMID:23959013

Villa-Ruano, Nemesio; Zurita-Vásquez, Guilibaldo G; Pacheco-Hernández, Yesenia; Betancourt-Jiménez, Martha G; Cruz-Durán, Ramiro; Duque-Bautista, Horacio

2013-01-01

289

New insights into the antioxidant activity of hydroxycinnamic acids: Synthesis and physicochemical characterization of novel halogenated derivatives  

Microsoft Academic Search

An interdisciplinary research project was developed combining the synthesis of a series of hydroxycinnamic acid derivatives and the evaluation of their physicochemical parameters (namely redox potentials and partition coefficients), along with the corresponding antioxidant activity. A structure–property–activity relationship (SPAR) approach was then applied aiming at establishing a putative relation between the physicochemical parameters of the compounds under study and their

Alexandra Gaspar; E. Manuela Garrido; Mário Esteves; Elias Quezada; Nuno Milhazes; Jorge Garrido; Fernanda Borges

2009-01-01

290

Effect of incorporating hydrothermal, kilned and defatted oats on antioxidant and chapatti making properties of wheat flour.  

PubMed

Oats were subjected to treatments like defatting, hydrothermal cooking and kilning, milled into flour and then the control and treated flours were incorporated into wheat flour at 25% and 50% levels and chapatti making behaviour and antioxidant properties were studied. The treatments significantly affected the antioxidant properties of oats. Incorporating oat flours to wheat increased total phenolic content but lowered the antioxidant activity however both were decreased significantly upon baking. The reducing power of the oat blended flour was higher than the wheat flours and ranged from 8.0 to 15.5 ?mol AAE/g and was further increased upon baking. The metal chelating activity of flour blends varied from 62.0% to 73.8% and further increased upon baking. After baking the total flavonoid content was lowered and ranged from 308 to 389 ?g CE/g. The non-enzymatic browning index significantly increased up to 27.6% upon baking. PMID:23411260

Gujral, Hardeep Singh; Sharma, Paras; Gill, Balmeet Singh; Kaur, Sumandeep

2012-11-08

291

Photochemical and Optical Properties of Water-Soluble Xanthophyll Antioxidants: Aggregation vs Complexation.  

PubMed

Xanthophyll carotenoids can self-assemble in aqueous solution to form J- and H-type aggregates. This feature significantly changes the photophysical and optical properties of these carotenoids, and has an impact on solar energy conversion and light induced oxidative damage. In this study we have applied EPR and optical absorption spectroscopy to investigate how complexation can affect the aggregation ability of the xanthophyll carotenoids zeaxanthin, lutein, and astaxanthin, their photostability, and antioxidant activity. It was shown that complexation with the polysaccharide arabinogalactan (AG) polymer matrix and the triterpene glycoside glycyrrhizin (GA) dimer reduced the aggregation rate but did not inhibit aggregation completely. Moreover, these complexants form inclusion complexes with both monomer and H-aggregates of carotenoids. H-aggregates of carotenoids exhibit higher photostability in aqueous solutions as compared with monomers, but much lower antioxidant activity. It was found that complexation increases the photostability of both monomers and the aggregates of xanthophyll carotenoids. Also their ability to trap hydroperoxyl radicals increases in the presence of GA as the GA forms a donutlike dimer in which the hydrophobic polyene chain of the xanthophylls and their H-aggregates lies protected within the donut hole, permitting the hydrophilic ends to be exposed to the surroundings. PMID:23937515

Polyakov, Nikolay E; Magyar, Adam; Kispert, Lowell D

2013-08-26

292

Antioxidant properties of 2-O-beta-D-glucopyranosyl-L-ascorbic acid.  

PubMed

The antioxidant activity of a provitamin C agent, 2-O-beta-D-glucopyranosyl-L-ascorbic acid (AA-2betaG), was compared to that of 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) and ascorbic acid (AA) using four in vitro methods, 1,1-diphenyl-picrylhydrazyl (DPPH) radical-scavenging assay, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(*+))-scavenging assay, oxygen radical absorbance capacity (ORAC) assay, and 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis inhibition assay. AA-2betaG slowly and continuously scavenged DPPH radicals and ABTS(*+) in roughly the same reaction profiles as AA-2G, whereas AA quenched these radicals immediately. In the ORAC assay and the hemolysis inhibition assay, AA-2betaG showed similar overall activities to AA-2G and to AA, although the reactivity of AA-2betaG against the peroxyl radical generated in both assays was lower than that of AA-2G and AA. These data indicate that AA-2betaG had roughly the same radical-scavenging properties as AA-2G, and a comprehensive in vitro antioxidant activity of AA-2betaG appeared to be comparable not only to that of AA-2G but also to that of AA. PMID:18540110

Takebayashi, Jun; Yagi, Yasuyuki; Ishii, Rie; Abe, Shigeki; Yamada, Kazuhiko; Tai, Akihiro

2008-06-07

293

The in vivo antioxidant and antifibrotic properties of green tea (Camellia sinensis, Theaceae).  

PubMed

The in vivo antioxidant and antifibrotic properties of green tea (Camellia sinensis, Theaceae) were investigated with a study of carbon tetrachloride (CCl(4))-induced oxidative stress and hepatic fibrosis in male ICR mice. Oral administration of green tea extract at doses of 125, 625 and 1250 mg/kg for 8 weeks significantly reduced (p<0.05) the levels of thiobarbituric acid-reactive substances (TBARS) and protein carbonyls in the liver by at least 28% compared with that was induced by CCl(4) (1 mL/kg) in mice. Moreover, green tea extract administration significantly increased (p<0.05) the activities of catalase, glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rd) in the liver. Our study found that oral administration of green tea extract prevented CCl(4)-induced hepatic fibrosis, as evidenced by a decreased hydroxyproline level in the liver and a reduced incidence of hepatic fibrosis by histological observations. These results indicate that green tea exhibits potent protective effects against CCl(4)-induced oxidative stress and hepatic fibrosis in mice by inhibiting oxidative damage and increasing antioxidant enzyme activities. PMID:23194532

Tsai, Chia-Fang; Hsu, Yu-Wen; Ting, Hung-Chih; Huang, Chun-Fa; Yen, Cheng-Chieh

2012-09-28

294

Palynological origin, phenolic content, and antioxidant properties of honeybee-collected pollen from Bahia, Brazil.  

PubMed

The aim of this study was to determine the palynological origin, phenolic and flavonoid content, and antioxidant properties of twenty-five samples of bee pollen harvested during a nine-month period (February-November) from the Canavieiras municipality (northeastern Brazil). Of the 25 samples analyzed, only two (February 01 and 02) were heterofloral. The predominant pollens in the samples analyzed during that month were: Cecropia, Eucalyptus, Elaeis, Mimosa pudica, Eupatorium, and Scoparia. Ethyl acetate fractions were analyzed by HPLC-DAD. The flavonoids isoquercetin, myricetin, tricetin, quercetin, luteolin, selagin, kaempferol, and isorhamnetin were detected. The flavonoid present in all 22 samples was isolated and identified as isorhamnetin 3-O-b-neohesperidoside. The total phenolic contents determined using the Folin-Ciocalteu reagent ranged from 41.5 to 213.2 mg GAE/g. Antioxidant activities based on the 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azinobis 3-ethylbenzothiozoline-6-sulfonic acid (ABTS), and Fe2+ ion chelating activity assays were observed for all extracts, and correlated with the total phenolic content. PMID:22314384

Freire, Kristerson R L; Lins, Antonio C S; Dórea, Marcos C; Santos, Francisco A R; Camara, Celso A; Silva, Tania M S

2012-02-07

295

Antioxidant versus anti-diabetic properties of leaves from Vernonia amygdalina Del. growing in Malaysia.  

PubMed

The antioxidant and anti-diabetic properties of the sequential extracts of Vernonia amygdalina based on the chemical composition of the most effective anti-diabetic extract were studied. Using DPPH and ABTS radical scavenging as well as FRAP assays, the extracts showed a consistent dose-dependent trend of potent antioxidant activity in the following solvents: water extract>methanol extract>chloroform extract>and petroleum ether extracts. In the oral glucose tolerance test, the chloroform extract exerted the highest response (33.3%), similar to metformin (27.2%), after 2h compared to the control (50.8%, P<0.05). After a 14-day administration in diabetic rats, the chloroform extract recorded the highest blood (23.5%) and serum (21.4%) glucose-lowering effects (P<0.05). GC-MS analysis of the chloroform extract revealed high levels of linoleic acid (4.72%), ?-linolenic acid (10.8%) and phytols (12.0%), as well as other compounds. PMID:23993503

Atangwho, Item J; Egbung, Godwin E; Ahmad, Mariam; Yam, Mun F; Asmawi, Mohd Z

2013-06-20

296

Antioxidant and signal modulation properties of plant polyphenols in controlling vascular inflammation.  

PubMed

Oxidized low-density lipoproteins (oxLDL) play a critical role in the initiation of atherosclerosis through activation of inflammatory signaling. In the present work we investigated the role of antioxidant and signal modulation properties of plant polyphenols in controlling vascular inflammation. Significant decrease in intracellular NO level and superoxide overproduction was found in human umbilical vein endothelial cells (HUVEC) treated with oxLDL, but not with LDL. The redox imbalance was prevented by the addition of quercetin or resveratrol. Expression analysis of 14 genes associated with oxidative stress and inflammation revealed oxLDL-mediated up-regulation of genes specifically involved in leukocyte recruitment and adhesion. This up-regulation could be partially avoided by the addition of verbascoside or resveratrol, while treatment with quercetin resulted in a further increase in the expression of these genes. Lipopolysaccharide (LPS)-treated HUVEC were also used for the evaluation of anti-inflammatory potency of plant polyphenols. Significant differences between HUVEC treaded with oxLDL and LPS were found in both the expression pattern of inflammation-related genes and the effects of plant polyphenols on cellular responses. The present data indicate that plant polyphenols may affect vascular inflammation not only as antioxidants but also as modulators of inflammatory redox signaling pathways. PMID:21371465

Kostyuk, Vladimir A; Potapovich, Alla I; Suhan, Tatyana O; de Luca, Chiara; Korkina, Liudmila G

2011-03-01

297

Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC.  

PubMed Central

Microbial infections of various types of wounds are a challenge to the treatment of wounds and wound healing. The study was to investigate antimicrobial and antioxidant properties of methanol leaf and stem bark extracts of Kigelia africana and methanol leaf and root extracts of Strophanthus hispidus and also to determine wound healing properties of the extracts. The antimicrobial activities of the methanol extracts were determined against two Gram-positive and two Gram-negative bacteria and a fungus using agar diffusion and micro-dilution methods. The antioxidant activity was determined using 1,1-diphenyl-2-picryl–hydrazyl (DPPH) method. The influence of the extracts on rate of wound closure was investigated using the excision wound model and histopathological investigation of treated and untreated wound tissues performed. The MICs of leaf extract of K. africana against test organisms were 2.5–7.5?mg/mL and stem bark extract were 2.25–7.5?mg/mL. The leaf extract of S. hispidus had MIC range of 2.5–7.5?mg/mL and 2.5–10?mg/mL for root extract. The IC50 of leaf and stem bark extracts of K. africana were 56.9 and 13.7??g/mL, respectively and leaf and root of S. hispidus were 49.8 and 45.1??g/mL, respectively. K. africana extracts (7.5% w/w) showed significant (P < 0.05) wound contraction at day 7 with 72% of wound closure whiles significant (P < 0.05) wound contractions were observed on day 11 for stem bark of K. africana, leaf and root extracts of S. hispidus. Wound tissues treated with the extracts showed improved collagenation, re-epitheliazition and rapid granulation formation compared with untreated wound tissues. The extracts were found to contain alkaloids, saponins, tannins, flavonoids, carbohydrates, and sapogenetic glycosides. The HPLC finger-printing of the extracts were developed. The leaf, stem bark and root extracts of K. africana and S. hispidus exhibited antimicrobial, antioxidant, and enhanced wound healing properties and these may justify the medicinal uses of the plants for treatment of microbial infections and wounds.

Agyare, Christian; Dwobeng, Anita Serwaa; Agyepong, Nicholas; Boakye, Yaw Duah; Mensah, Kwesi Boadu; Ayande, Patrick George; Adarkwa-Yiadom, Martin

2013-01-01

298

Assessing the effects of different pectins addition on color quality and antioxidant properties of blackberry jam  

PubMed Central

Background In the last years pectin and other hydrocolloids were tested for improving the color stability and the retention of bioactive compounds in gelled fruit-based products. In line with these concerns, our study has been directed to quantify the changes in antioxidant status and color indices of blackberry jam obtained with different types of pectin (degree of esterification: DE, degree of amidation: DA) and doses in response to processing and storage for 1, 3 and 6 months at 20°C. Results Blackberry jam was obtained by a traditional procedure used in households or small-scale systems with different commercial pectins (HMP: high-methoxyl pectin, LMP: low-methoxyl pectin and LMAP: low-methoxyl amidated pectin) added to three concentrations (0.3, 0.7 and 1.0%) and investigated in terms of total monomeric anthocyanins (TMA), antioxidant capacity expressed as ferric reducing antioxidant power (FRAP), total phenolics (TP), color density (CD) and percent of polymeric color, PC (%). Thermal processing resulted in significant depreciation of analyzed parameters reported to the corresponding values of fresh fruit as follows: TMA (69-82%), TP (33-55%) and FRAP (18-52%). Biologically active compounds and color were best retained one day post-processing in jams with LMAP followed by samples with LMP and HMP. Storage for 6 months brings along additional dramatic losses reported to the values recorded one day post-processing as follows: TMA (31-56%), TP (29-51%) and FRAP (20-41%). Also, both processing and storage resulted in significant increases in PC (%). The pectin type and dosage are very influential factors for limiting the alterations occurring in response to processing and storage. The best color retention and the highest TMA, TP and FRAP were achieved by LMAP, followed by LMP and HMP. Additionally, a high level of bioactive compounds in jam could be related to a high dose of pectin. LMAP to a level of 1% is the most indicated to provide the highest antioxidant properties in jam. Conclusions The retention of bioactive compounds and jam color stability were strongly dependent on the pectin type and dosage. By a proper selection of pectin type and dose could be limited the losses recorded in response to processing and storage.

2013-01-01

299

New C-deoxyhexosyl flavones and antioxidant properties of Passiflora edulis leaf extract.  

PubMed

The flavonoids present in passion fruit (Passiflora edulis) leaves were identified by a high-performance liquid chromatography-diode array detection-tandem mass spectrometry (HPLC-DAD-MS/MS) method. Sixteen apigenin or luteolin derivatives were characterized, which included four mono-C-glycosyl, eight O-glycosyl- C-glycosyl, and four O-glycosyl derivatives. With the exceptions of C-hexosyl luteolin and C-hexosyl apigenin, all the compounds exhibited a deoxyhexose moiety. Moreover, the uncommon C-deoxyhexosyl derivatives of luteolin and apigenin have been identified for first time in P. edulis by HPLC-DAD-MS/MS. The antioxidative capacity of passion fruit leaves was checked against DPPH radical and several reactive oxygen species (superoxide radical, hydroxyl radical, and hypochlorous acid), revealing it to be concentration-dependent, although a pro-oxidant effect was noticed for hydroxyl radical. PMID:18001037

Ferreres, Federico; Sousa, Carla; Valentão, Patrícia; Andrade, Paula B; Seabra, Rosa M; Gil-Izquierdo, Angel

2007-11-15

300

Isolation, characterization and antioxidative effect of phyllanthin against CCl4-induced toxicity in HepG2 cell line.  

PubMed

The present study was an attempt to investigate the hepatoprotective and antioxidative property of Phyllanthus amarus (P. amarus) extract and phyllanthin. Phyllanthin, one of the active lignin present in this plant species was isolated from the aerial parts, by silica gel column chromatography employing gradient elution with hexane-ethyl acetate solvent mixture. It was obtained in high yields (1.23%), compared to reported procedures and the purity was ascertained by HPTLC and reversed-phase HPLC analysis. Characterization of phyllanthin was done by mp, UV-Visible spectrophotometry, elemental analysis, FT-IR, 1H NMR, 13C NMR and mass spectral analysis. Free radical scavenging activity of P. amarus extract and phyllanthin was also examined using DPPH assay. The protective effect of P. amarus extract and phyllanthin was studied on CCl4-induced toxicity in human hepatoma HepG2 cell line. The results indicated that CCl4 treatment caused a significant decrease in cell viability. In addition, the toxin treatment initiated lipid peroxidation (LPO), caused leakage of enzymes like alanine transaminase (ALT) and lactate dehydrogenase (LDH) with a significant decrease in glutathione (GSH) levels. It was observed that phyllanthin effectively alleviated the changes induced by CCl4 in a concentration-dependent manner, with much smaller strengths as compared to P. amarus extract. PMID:19576190

Krithika, Rajesh; Mohankumar, Ramasamy; Verma, Ramtej J; Shrivastav, Pranav S; Mohamad, Illiyas L; Gunasekaran, Palani; Narasimhan, Srinivasan

2009-07-01

301

NMR CHARACTERIZATIONS OF PROPERTIES OF HETEROGENEOUS MEDIA  

SciTech Connect

A critical and long-standing need within the petroleum industry is the specification of suitable petrophysical properties for mathematical simulation of fluid flow in petroleum reservoirs (i.e., reservoir characterization). The development of accurate reservoir characterizations is extremely challenging. Property variations may be described on many scales, and the information available from measurements reflect different scales. In fact, experiments on laboratory core samples, well-log data, well-test data, and reservoir-production data all represent information potentially valuable to reservoir characterization, yet they all reflect information about spatial variations of properties at different scales. Nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI) provide enormous potential for developing new descriptions and understandings of heterogeneous media. NMR has the rare capability to probe permeable media non-invasively, with spatial resolution, and it provides unique information about molecular motions and interactions that are sensitive to morphology. NMR well-logging provides the best opportunity ever to resolve permeability distributions within petroleum reservoirs. We develop MRI methods to determine, for the first time, spatially resolved distributions of porosity and permeability within permeable media samples that approach the intrinsic scale: the finest resolution of these macroscopic properties possible. To our knowledge, this is the first time that the permeability is actually resolved at a scale smaller than the sample. In order to do this, we have developed a robust method to determine of relaxation distributions from NMR experiments and a novel implementation and analysis of MRI experiments to determine the amount of fluid corresponding to imaging regions, which are in turn used to determine porosity and saturation distributions. We have developed a novel MRI experiment to determine velocity distributions within flowing experiments, and developed methodology using that data to determine spatially resolved permeability distributions. We investigate the use of intrinsic properties for developing improved correlations for predicting permeability from NMR well-logging data and for obtaining more accurate estimates of multiphase flow properties--the relative permeability and capillary pressure--from displacement experiments. We demonstrate the use of MRI measurements of saturation and relaxation for prediction wetting-phase relative permeability for unstable experiments. Finally, we developed an improved method for determining surface relaxivity with NMR experiments, which can provide better descriptions of permeable media microstructures and improved correlations for permeability predictions.

C.T. Philip Chang; Changho Choi; Jeromy T. Hollenshead; Rudi Michalak; Jack Phan; Ramon Saavedra; John C. Slattery; Jinsoo Uh; Randi Valestrand; A. Ted Watson; Song Xue

2005-01-01

302

Addition of bovine plasma hydrolysates improves the antioxidant properties of soybean and sunflower protein-based films  

Microsoft Academic Search

The effect of adding different amounts of a bovine plasma hydrolysate (BPH) with high antioxidant capacity on the functional properties of protein films based on soybean and sunflower protein was studied. BPH caused a decrease in tensile strength, elastic modulus and glass transition temperature of the films, as well as an increase in their elongation at break and water vapor

Pablo R. Salgado; Graciela B. Fernández; Silvina R. Drago; Adriana N. Mauri

2011-01-01

303

Sulfation of a polysaccharide produced by a marine filamentous fungus Phoma herbarum YS4108 alters its antioxidant properties in vitro  

Microsoft Academic Search

Free radicals and other reactive oxygen species (ROS) are generated by all aerobic cells and are widely believed to play a significant role in aging as well as a number of degenerative or pathological diseases. This study compared the free radical-scavenging properties and antioxidant activity of YCP, a polysaccharide from the mycelium of a marine filamentous fungus Phoma herbarum YS

X. B. Yang; X. D. Gao; F. Han; R. X. Tan

2005-01-01

304

Structurally related flavonoids with antioxidative properties differentially affect cell cycle progression and apoptosis of human acute leukemia cells  

Microsoft Academic Search

Flavonoids are a group of common phenolic compounds that are ubiquitous in all vascular plants and are important constituents of the human diet. The possible relationship between the chemical structure of 6 closely related naturally occurring flavonoids and their antioxidative properties as well as their effects on proliferation, apoptosis, and cycle of human acute leukemia (HL-60) cells were investigated. Quercetin,

Gordana Rusak; Herwig O. Gutzeit; Jutta Ludwig Müller

2005-01-01

305

Influence of Processing on Bioactive Substances Content and Antioxidant Properties of Apple Purée from Organic and Conventional Production in Poland  

Microsoft Academic Search

The organic food market is developing dynamically in many European countries and therefore studies concerning the nutritive value of organically produced foods are becoming increasingly important. It was found appropriate to conduct studies on selected bioactive substances and antioxidant properties of apple preserves prepared from organic vs. conventional apples. Three apple cultivars, Lobo, Boskoop and Cortland, were grown in organic

306

Fermentation Changes the Nutritive Value, Polyphenol Distribution, and Antioxidant Properties of Parkia biglobosa Seeds (African Locust Beans)  

Microsoft Academic Search

This present study was carried out to investigate the effect of natural fermentation on the nutritive value (proximate composition), polyphenolic distribution, and antioxidant properties of African locust beans (Parkia biglobosai). African locust bean (ALB) seeds were boiled for 12 h, dehulled and subjected to natural solid substrate fermentation without any inoculation for 4 days. The proximate composition of the fermented

G. Oboh; K. B. Alabi; A. A. Akindahunsi

2008-01-01

307

Antioxidative properties of functional polyphenols and their metabolites assessed by an ORAC assay.  

PubMed

We compared the antioxidative activities of polyphenol metabolites with those of intact functional polyphenols by an assay of the oxygen radical absorbance capacity (ORAC). The metabolites of ellagitannin geraniin, chlorogenic acid, and (-)-epigallocatechin gallate displayed more potent antioxidative activity than their respective original compounds. Our findings suggest that these metabolites may play important roles as biological antioxidants after their consumption. PMID:22313776

Ishimoto, Hidekazu; Tai, Akihiro; Yoshimura, Morio; Amakura, Yoshiaki; Yoshida, Takashi; Hatano, Tsutomu; Ito, Hideyuki

2012-02-07

308

Growth, characterization, and properties of nanographene.  

PubMed

A systematic study on nanographene grown directly on silicon dioxide substrates is reported. The growth is carried out in a remote plasma-enhanced chemical vapor deposition system at a low temperature of around 550 °C with methane gas as the carbon source. Atomic force microscopy is used to characterize the nanographene morphology, and Raman spectroscopy, X-ray photoelectron spectroscopy, and scanning tunneling microscopy are exploited to identify the in-plane sp(2) bonding structures of nanographene samples. Electrical transport properties are measured at various temperatures down to 4 K. Tunneling effects, minimal conductance at the charge-neutral point, sheet resistances, and Dirac point position at different channel lengths are investigated. In addition, nanographene film possesses high transmittance properties, as indicated by transmittance spectra. Nanographene devices are fabricated from rippled structures, and show great promise for strain-gauge sensor applications. PMID:22378609

Yang, Wei; He, Congli; Zhang, Lianchang; Wang, Yi; Shi, Zhiwen; Cheng, Meng; Xie, Guibai; Wang, Duoming; Yang, Rong; Shi, Dongxia; Zhang, Guangyu

2012-02-29

309

Sulfation of genistein alters its antioxidant properties and its effect on platelet aggregation and monocyte and endothelial function  

Microsoft Academic Search

Soy isoflavones have been extensively studied because of their possible benefits to human health. Genistein, the major isoflavone aglycone, has received most attention; however, it undergoes extensive metabolism (e.g. conjugation with sulfuric acid) in the gut and liver, which may affect its biological properties. This study investigated the antioxidant activity and free radical-scavenging properties of genistein, genistein-4?-sulfate and genistein-4?-7-disulfate as

Gerald Rimbach; Peter D Weinberg; Sonia de Pascual-Teresa; Maria Garcia Alonso; Ben A Ewins; Rufus Turner; Anne Marie Minihane; Nigel Botting; Brian Fairley; Seiichi Matsugo; Yuzo Uchida; Aedin Cassidy

2004-01-01

310

Evaluation of antioxidative properties of Allium species growing wild in Italy.  

PubMed

The genus Allium (Alliaceae) is an important dietary source of antioxidant phytochemical products. The antioxidant activity of some Allium species is well known but no information is available on the in vitro antioxidant activities of Italian Allium species growing wild. The aim of this study was to examine the in vitro antioxidant activity of aqueous extracts of different parts belonging to three Allium species growing wild, endemic to Italian flora: Allium neapolitanum Cyr., Allium subhirsutum L. and Allium roseum L., compared with the in vitro antioxidant activity of aqueous extracts of bulbs and leaves of the greatly studied garlic (Allium sativum L.). The antioxidant potential of extracts was evaluated using two different spectrophotometric assays: the DPPH test and FRAP assay. Furthermore the polyphenolic content was determined in all Allium species. The flowers of species growing wild showed the higher antioxidant power. Interesting results were shown even by the leaves, while the antioxidant capacity of the bulbs was lower. A correlation between the phenolic contents and the antioxidant activity was discovered. The differences in antioxidant capacity reflect the variability in the Allium species and the parts of the plant used and that the bulb of Allium sativum did not show higher antioxidant power. PMID:17514631

Nencini, Cristina; Cavallo, Federica; Capasso, Anna; Franchi, Gian Gabriele; Giorgio, Giorgi; Micheli, Lucia

2007-09-01

311

Honeybee-collected pollen from five Portuguese Natural Parks: palynological origin, phenolic content, antioxidant properties and antimicrobial activity.  

PubMed

The aim of this study was to determine the palynological origin, phenolic content, antioxidant and antimicrobial properties of pollen from five Portuguese Natural-Parks [Parque Nacional Peneda Gerês (PNPG); Parque Natural do Montesinho (PNM); Parque Natural do Alvão (PNA); Parque Natural da Serra da Estrela (PNSE) and Parque Natural do Douro Internacional (PNDI)]. Eight families were found in the mixture of bee pollen: Rosaceae, Cistaceae, Boraginaceae, Asteraceae, Fagaceae, Ericaeae, Myrtaceae and Fabaceae. The phenolic compounds content, determined by the Folin-Ciocalteu method, varied between 10.5 and 16.8 mg of gallic acid equivalents/g of extract (mg GAE/g) found in bee pollen from PNM and PNDI, respectively. The free radical scavenging measured showed the highest effective extract - PNM with EC(50) 2.16, followed by PND with 2.24 mg/mL. In the ?-carotene bleaching assay the same behaviour as in the DPPH method was verified. We also verified that the presence of pollen differentially affected the growth of bacteria Gram-positive, Gram-negative and yeasts under study, depending this on the microorganism and the pollen used. This is an important study since, as far we know, it is the first time that Portuguese bee pollen from Natural Parks was studied, and their characterization can increase their economic value. PMID:21291944

Morais, Margarida; Moreira, Leandro; Feás, Xesús; Estevinho, Leticia M

2011-02-01

312

Quantitative electron spin resonance (ESR) analysis of antioxidative properties using the acetaldehyde/xanthine oxidase system  

NASA Astrophysics Data System (ADS)

We present a method for the quantitative ESR analysis of the antioxidant properties of drugs using the acetaldhehyde/xanthine oxidase (AC/XOD) superoxide generating system and 5,5-dimethyl-l-pyrroline-N-oxide (DMPO) as spin trap. In stoichiometric conditions (AC/XOD, 60 mM/0.018 U), the resulting paramagnetic DMPO adduct disappeared with superoxide dismutase and remained when catalase or DMSO were used. That adduct was dependent only on superoxide and resulted from the trapping of a carboxyl radical by DMPO (aN = 15.2 G, aH = 18.9 G). Similar results were obtained using 4-pyridyl-l-oxide-N-t-butyl nitrone (POBN) as spin trap. The ESR signal of the DMPO-CO2- adduct was very stable and allowed quantitative analysis of the antioxidative activity of redox molecules from an IC{50} value representing the concentration causing 50% inhibition of its intensity. Among the tested compounds, manganese(II), complexes were the most effective, 25 times as active as ascorbic acid or (+)catechin and 500-fold more antioxidative than Trolox^R. Nous présentons une méthode d'analyse quantitative de l'activité antioxydante de composés d'intérêt pharmaceutique basée sur le système acétaldéhyde/xanthine oxydase (AC/XOD), l'utilisation de la RPE et du piégeage de spin avec le 5,5-diméthyl-l-pyrroline-N-oxyde (DMPO). Dans les conditions stoechiométriques {AC/XOD, 60 mM/0,018 U/ml}, l'adduit radicalaire résultant de ce système disparaît en présence de superoxyde dismutase et persiste en présence de catalase ou de DMSO. Cet adduit ne dépend que de la présence de l'anion superoxyde et provient du piégeage d'un radical carboxyle CO2- sur le DMPO (aN = 15.2 G, aH = 18.9 G). Des résultats similaires ont été obtenus avec le piégeur de spin 4-pyridyl-l-oxyde-N-t-butyl nitrone (POBN). Le signal RPE de l'adduit DMPO-CO2- est très stable et permet la quantification de l'activité antioxydante de pharmacophores redox par la détermination de la CI{50}, concentration qui diminue de 50 % son intensité. Parmi les composés testés, les complexes du manganèse sont les plus antioxydants, 25 fois plus actifs que la vitamine C ou la catéchine(+), 500 fois plus antioxydants que le Trolox^R.

Souchard, J.-P.; Nepveu, F.

1998-05-01

313

Comparison of the antioxidant properties of HYAFF-11p75, AQUACEL and hyaluronan towards reactive oxygen species in vitro.  

PubMed

In chronic wounds, a number of host factors are released which perpetuate the inflammatory process, including polymorphonuclear leukocyte (PMN)-derived reactive oxygen species (ROS), such as superoxide radical (O2*-) and hydroxyl radical (*OH) species. The glycosaminoglycan. hyaluronan, has been shown to act as an antioxidant towards ROS, although the potential for biomaterials, such as HYAFF -11p75 (the 75% benzyl ester of hyaluronan) and AQUACEL (carboxymethylcellulose), to act in this manner has yet to be elucidated. This study compared the antioxidant properties of high and low molecular weight hyaluronan (HMWT HA and LMWT HA), HYAFF -11p75, AQUACEL and an AQUACEL /hyaluronan composite (AQUACEL /HA) against O2*- and *OH. The antioxidant capacities of each material were assessed by their ability to inhibit cytochrome C reduction by O2*- fluxes, generated via the oxidation of hypoxanthine by xanthine oxidase, and their inhibition of 2-deoxy-D-ribose degradation by *OH fluxes, generated by the reaction of hydrogen peroxide (H2O2) and iron (Fe2+). All materials studied possessed dose dependent antioxidant properties towards O2*-, with HYAFF 11p75 having the greatest antioxidant potential towards these species, followed by AQUACEL, HMWT HA, AQUACEL /HA and LMWT HA. Only HMWT HA exhibited dose dependent antioxidant properties towards *OH at the fluxes examined. Gas chromatography/mass spectrometry analysis implied that ester bonds between the hyaluronan backbone and benzyl groups of HYAFF -11p75 are highly susceptible to O2*- hydrolysis, with the de-esterified benzyl alcohol being rapidly degraded in the presence of *OH. This data supports the hypothesis that HYAFF -11p75 has greater antioxidant capacity towards O2*-, due to the esterified benzyl groups providing alternative sites for O2*- attack other than the hyaluronan backbone of HYAFF -11p75 itself and explains the inability of HYAFF -11p75 to scavenge *OH, due to benzyl alcohol degradation by *OH. The antioxidant activities of these biomaterials, particularly HYAFF -11p75 and AQUACEL, towards O2*- could be beneficial, as the scavenging of PMN-derived O2*- may remove initial sources of O2*- and further prevent the secondary formation of *OH. These ROS are thought to be a primary causal factor for the extensive degradation and metabolic alterations observed in chronic wounds. PMID:11962667

Moseley, R; Leaver, M; Walker, M; Waddington, R J; Parsons, D; Chen, W Y J; Embery, G

2002-05-01

314

Multivariate calibration by near infrared spectroscopy for the determination of the vitamin E and the antioxidant properties of quinoa.  

PubMed

Quinoa is a pseudocereal that is grown mainly in the Andes. It is a functional food supplement and ingredient in the preparation of highly nutritious food. In this paper we evaluate the potential of near infrared spectroscopy (NIR) for the determination of vitamin E and antioxidant capacity in the quinoa as total phenol content (TPC), radical scavenging activity by DPPH (2,2-diphenyl-2-picryl-hydrazyl) and cupric reducing antioxidant capacity (CUPRAC) expressed as gallic acid equivalent (GAE). For recording NIR a fiber optic remote reflectance probe applied directly on the quinoa samples without treatment was used. The regression method used was modified partial least squares (MPLS). The multiple correlation coefficients (RSQ) and the standard prediction error corrected (SEP(C)) were for the vitamin E (0.841 and 1.70mg100g(-1)) and for the antioxidants TPC (0.947 and 0.08mgGAEg(-1)), DPPH radical (0.952 and 0.23mgGAEg(-1)) and CUPRAC ( 0.623 and 0.21mgGAEg(-1)), respectively. The prediction capacity of the model developed measured by the ratio performance deviation (RPD) for vitamin E (2.51), antioxidants TPC (4.33), DPPH radical (4.55) and CUPRAC (1.55) indicated that NIRS with a fiber optic probe provides an alternative for the determination of vitamin E and antioxidant properties of the quinoa, with a lower cost, higher speed and results comparable with the chemical methods. PMID:24148374

Moncada, Guillermo Wells; González Martín, Ma Inmaculada; Escuredo, Olga; Fischer, Susana; Míguez, Montserrat

2013-05-04

315

Effect of genotype, environment, and their interaction on phytochemical compositions and antioxidant properties of soft winter wheat flour.  

PubMed

The effect of genotype (G), growing environment (E), and their interaction (G×E) on the antioxidant properties and chemical compositions were investigated using the flour samples of 10 wheat varieties grown in four different locations in Maryland. Lutein content of wheat flour ranged from 0.10 to 0.69 ?g/g, and ?-tocopherol ranged from 0.12 to 0.83 ?g/g. Total carotenoids were primarily affected by E (45.7%), while G×E interaction had a larger effect on the level of total tocopherols (71.6%). E had the largest effect on antioxidant activity against oxygen, hydroxyl, and ABTS(·+) radicals. G had the least influence on the measured phytochemicals and antioxidant activity assays. Total carotenoids had a significant correlation with average low air temperature (r=0.359, p<0.01) as well as precipitation level (r=0.214, p<0.01). ABTS(·+) radical scavenging capacity had a positive correlation with average air temperature (r=0.705, p<0.01), while hydroxyl radical scavenging capacity had a negative correlation with temperature (r=-0.269. p<0.01). These results show that environment, genotype, and their interaction could influence the levels of lipophilic antioxidants and antioxidant activities of wheat flour. PMID:23265511

Lv, Junli; Lu, Yingjian; Niu, Yuge; Whent, Monica; Ramadan, Mohamed Fawzy; Costa, Jose; Yu, Liangli Lucy

2012-11-10

316

Effect of extrusion cooking on the physicochemical properties, resistant starch, phenolic content and antioxidant capacities of green banana flour.  

PubMed

Green banana flour was extruded through a co-rotating twin-screw extruder with constant barrel temperature. The objectives of this study were to determine the effect of extrusion cooking variables (feed moisture, FM, 20% and 50%; screw speed, SS, 200 and 400rpm) and storing of the extruded flours at 4°C for 24h on the physicochemical properties, resistant starch (RS), pasting properties and antioxidant capacities. Extrusion cooking at higher FM and lower SS increased the amylose content, which was expressed in highest RS content. Water adsorption index (WAI) and pasting properties were increased, while water solubility index (WSI), total phenolic content (TPC) and antioxidant activities (FRAP, ABTS(+), DPPH) in free and bound phenolics were decreased compared to the other extruded samples. Storing the extruded flours at 4°C for 24h prior to oven drying was the main factor leading to a further increase in the content of amylose, RS, TPC and WSI values, as well as pasting properties - in particular peak viscosity. Compared to native banana flour, extrusion cooking caused significant changes in all studied properties of the extruded flours, except for soluble DF and antioxidant capacity (ABTS(+) and DPPH) of bound phenolics. PMID:24054209

Sarawong, Chonthira; Schoenlechner, Regine; Sekiguchi, Ken; Berghofer, Emmerich; Ng, Perry K W

2013-07-27

317

Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro  

PubMed Central

Objective To evaluate and compare antioxidant activities of the aqueous extracts of unripe plantain (Musa paradisiaca), assess their inhibitory action on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro and to characterize the main phenolic constituents of the plantain products using gas chromatography analysis. Methods Aqueous extracts of plantain products (raw, elastic pastry, roasted and boiled) flour of 0.1 g/mL (each) were used to determine their total phenol, total flavonoid, 1,1 diphenyl-2 picrylhydrazyl (DPPH) and hydroxyl (OH) radical scavenging ability. The inhibitory effect of the extracts on sodium nitroprusside induced lipid peroxidation was also determined. Results The results revealed that all the aqueous extracts showed antioxidant activity. The boiled flour had highest DPPH and OH radical scavenging ability while raw flour had the highest Fe2+ chelating ability, sodium nitroprusside inhibitory effect and vitamin C content. The antioxidant results showed that elastic pastry had the highest total phenol and total flavonoid content. Characterization of the unripe plantain products for polyphenol contents using gas chromatography showed varied quantity of apigenin, myricetin, luteolin, capsaicin, isorhaemnetin, caffeic acid, kampferol, quercetin, p-hydroxybenzoic acid, shogaol, glycitein and gingerol per product on the spectra. Conclusions Considering the antioxidant activities and ability to inhibit lipid peroxidation of unripe plantain, this could justify their traditional use in the management/prevention of diseases related to stress.

Shodehinde, Sidiqat Adamson; Oboh, Ganiyu

2013-01-01

318

Characterization of the antioxidant composition of strawberry tree ( Arbutus unedo L.) fruits  

Microsoft Academic Search

The fruits of the strawberry tree (Arbutus unedo L.) are consumed mainly as processed product, but may be a good source of antioxidants if consumed as fresh fruit. The aim of the present study was to identify and quantify the antioxidant components present in strawberry tree fruits, including flavonoids, vitamins C and E and carotenoids. The fruits are a very

K. Pallauf; J. C. Rivas-Gonzalo; M. D. del Castillo; M. P. Cano; S. de Pascual-Teresa

2008-01-01

319

Studies on the Food Additive Propyl Gallate: Synthesis, Structural Characterization, and Evaluation of the Antioxidant Activity  

ERIC Educational Resources Information Center

|Antioxidants are additives largely used in industry for delaying, retarding, or preventing the development of oxidative deterioration. Propyl gallate (E310) is a phenolic antioxidant extensively used in the food, cosmetics, and pharmaceutical industries. A series of lab experiments have been developed to teach students about the importance and…

Garrido, Jorge; Garrido, E. Manuela; Borges, Fernanda

2012-01-01

320

Physicochemical characterization, antioxidant and anticancer activities in vitro of a novel polysaccharide from Melia toosendan Sieb. Et Zucc fruit  

Microsoft Academic Search

A novel water-soluble polysaccharide pMTPS-3, obtained from Melia toosendan Sieb. Et Zucc fruit by hot-water extraction and ethanol precipitation, was fractionated by DEAE-52 cellulose anion-exchange and Sephadex G-100 gel filtration chromatography. Its primary structural features and molecular weight were characterized by Fourier infrared spectrometry (FTIR), gel permeation chromatography (GPC) and gas chromatography (GC). And the antioxidant activities of pMTPS-3 in

Liang He; Pengfei Ji; XingGuo Gong; Weiqi Li; Junwen Cheng; Hua Qian; XianLiang Song

2011-01-01

321

Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays.  

PubMed

Aqueous extracts of 30 plants were investigated for their antioxidant properties using DPPH and ABTS radical scavenging capacity assay, oxygen radical absorbance capacity (ORAC) assay, superoxide dismutase (SOD) assay, and ferric reducing antioxidant potential (FRAP) assay. Total phenolic content was also determined by the Folin-Ciocalteu method. Antioxidant properties and total phenolic content differed significantly among selected plants. It was found that oak (Quercus robur), pine (Pinus maritima), and cinnamon (Cinnamomum zeylanicum) aqueous extracts possessed the highest antioxidant capacities in most of the methods used, and thus could be potential rich sources of natural antioxidants. These extracts presented the highest phenolic content (300-400 mg GAE/g). Mate (Ilex paraguariensis) and clove (Eugenia caryophyllus clovis) aqueous extracts also showed strong antioxidant properties and a high phenolic content (about 200 mg GAE/g). A significant relationship between antioxidant capacity and total phenolic content was found, indicating that phenolic compounds are the major contributors to the antioxidant properties of these plants. PMID:19199445

Dudonné, Stéphanie; Vitrac, Xavier; Coutière, Philippe; Woillez, Marion; Mérillon, Jean-Michel

2009-03-11

322

Characterizing Correctness Properties of Parallel Programs Using Fixpoints  

Microsoft Academic Search

We have shown that correctness properties of parallel programs can be described using computation trees and that from these descriptions fixpoint characterizations can be generated. We have also given conditions on the form of computation tree descriptions to ensure that a correctness property can be characterized using continuous fixpoints. A consequence is that a correctness property such as inevitability under

E. Allen Emerson; Edmund M. Clarke

1980-01-01

323

On quantitative characterization of microstructures and effective properties  

Microsoft Academic Search

Proper quantitative characterization of microstructures, for the purpose of modeling the effective properties, is discussed. This is a broad subject that covers different physical properties (elastic, conductive, transport, etc.), as well as various types of microstructures. The present work focuses on microstructures that can be characterized as continuous matrices containing isolated inhomogeneities of diverse shapes, properties and orientations. We address

Mark Kachanov; Igor Sevostianov

2005-01-01

324

Characterization of phenolic compounds, carotenoids, vitamins and antioxidant activities of selected Malaysian wild edible plants.  

PubMed

This study was carried out to characterize phenolic compounds, carotenoids, vitamins and the antioxidant activity of selected wild edible plants. Plant extracts were purified, and phenolic compounds comprising 11 phenolic acids (hydroxybenzoic acid and hydrocinnamic acid) and 33 flavonoids (including catechin, glycosides and aglycones) were analysed using High Performance Liquid Chromatography - Diode Array Detector (HPLC-DAD). Furthermore, the contents of ascorbic acid and tocopherol ((? and ? tocopherol) and carotenoids (lutein and ?-carotene) were also determined. The major phenolics identified consisted of glycosides of flavones (apigenin and luteolin) and flavonols (kaempferol and quercetin). Among the phenolic acids identified after hydrolysis, coumaric acid was the predominant phenolic acid in all the extracts of wild plants. Ascorbic acid [53.8 mg/100 g fresh weight (FW)] and ?-carotene (656.5 mg/100 g FW) showed the highest content in the leaf of Heckeria umbellatum. In conclusion, the leaves of H. umbellatum, Aniseia martinicensis and Gonostegia hirta have excellent potential in the future to emerge as functional ingredients. PMID:23368987

Wong, Jin Yi; Matanjun, Patricia; Ooi, Yasmin Beng Houi; Chia, Kah Fei

2013-02-01

325

Cytotoxic and antioxidative activities of Plantago lagopus L. and characterization of its bioactive compounds.  

PubMed

Bioactivity guided isolation and characterization of phytoconstituents from the aerial parts of Plantago lagopus L. were performed to give a new insight into the usage of Plantago species in traditional medicine. The extract showed strong radical scavenging effects against DPPH, nitric oxide (NO) and superoxide (SO) radicals comparable to that of known antioxidants 3-t-butyl-4-hydroxyanisole, ascorbic acid (vitamin C), and quercetin in addition to its cytotoxic activities against HEP-2 (human larynx epidermoid carcinoma) and RD (human rhabdomyosarcoma) cell lines based on MTT assay for growth inhibition. The gallic acid equivalent total phenolic content of the plant was found to be 79.94 mg/g dry extract. Phenylethanoid glycosides, verbascoside and calceorioside A were isolated from the most active fraction and both compounds showed strong radical scavenging activity against tested radicals and cytotoxicity against HEP-2, RD and MCF-7 (human breast adenocarcinoma) cell lines. In addition apoptotic cell death was observed in histological analysis. Taken together, these findings suggest that verbascoside and calceorioside A may be used in cancer prevention. PMID:22289578

Harput, U Sebnem; Genc, Yasin; Saracoglu, Iclal

2012-01-25

326

Structure–property studies on the antioxidant activity of flavonoids present in diet  

Microsoft Academic Search

The screening of natural flavonoids for their bioactivity as antioxidants is usually carried out by determinination of their profile as chain-breaking antioxidants, by the evaluation of their direct free radical-scavenging activity as hydrogen- or electron-donating compounds. Since this may not be the only mechanism underlying the antioxidant activity it is important to check the ability of these compounds to act

Susana Teixeira; Christophe Siquet; Carla Alves; Isabel Boal; M. Paula Marques; Fernanda Borges; José L. F. C. Lima; Salette Reis

2005-01-01

327

Antioxidant properties of dihydroherbimycin A from a newly isolated Streptomyces sp  

Microsoft Academic Search

During antioxidant screening using 1,1-diphenyl-picrylhydrazyl (DPPH) and a lipid peroxidation assay, a streptomycete strain\\u000a was found to produce herbimycin A and dihydroherbimycin A as antioxidants in the culture filtrate. These molecules were identified\\u000a by using spectral analyses, including infrared, ultraviolet, mass spectrum, and nuclear magnetic resonance assays. In the\\u000a DPPH radical-scavenging assay, dihydroherbimycin A exhibited more potent antioxidant activity (IC50,

Hung Bae Chang; Jae-heon Kim

2007-01-01

328

Functionality and antioxidant properties of tilapia (Oreochromis niloticus) as influenced by the degree of hydrolysis.  

PubMed

Freeze dried protein powders (Fresh minced meat, FMM and Hot water dip, HWD) from tilapia (Oreochromis niloticus) were hydrolyzed by Alcalase 2.4 L (Alc), Flavourzyme (Flav) and Neutrase (Neut), and investigated for antioxidant activity and their functional properties. FMM and HWD hydrolysed by Alc, exhibiting superior antioxidant activity, had estimated degrees of hydrolysis (DH) of 23.40% and 25.43%, respectively. The maximum values of the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 3-(2-pyridyl) 5,6-bis(4-phenyl-sulphonic acid)-1,2,4-triazine (ferrozine), radical scavenging activities and metal chelating properties were 86.67%, 91.27% and 82.57%, and 84.67%, 92.60% and 78.00% for FMM and HWD, respectively, with a significant difference (P < 0.05) between the samples. Essential amino acids were above the amounts recommended by the Food and Agricultural Organization/World Health Organization (FAO/WHO/UNU) for humans. Lower molecular weight sizes <3,000 Da were more predominant in FMM and HWD hydrolysed by Alc, while in hydrolysed by Flav and Neut they were >8,000 Da. At pH 2, FMM and HWD hydrolysates have varying solubilities above 85% (Alc FMM; 91.33%, Flav FMM; 79.5%, Neut FMM; 83.8% and Alc HWD; 90.45%, Flav HWD; 83.5%, and Neut HWD; 85.8%). They have 'U' shaped solubility curves, water holding capacity was in the range of 2.77 and 1.77 mL/g, while oil holding capacity ranged between 3.13 and 2.23 mL/g. FMM and HWD have the highest bulk density of 0.53 and 0.53 for Neutrase and Alcalase 2.4 L, respectively. Foam capacity and stability ranged from 125.5 to 61.4, 138.5 to 45.2, 130.0 to 62.5, and 124.5 to 55.0, 137.5 to 53.3, 129.6 to 62.7 for FMM and HWD hydrolyzed with Alcalase 2.4 L, Flavourzyme and Neutrase, respectively. Tilapia fish protein hydrolysates are thus potential functional food ingredients. PMID:20480046

Foh, Mohamed Beva Kelfala; Amadou, Issoufou; Foh, Betty Mabel; Kamara, Mohamed Tabita; Xia, Wenshui

2010-04-26

329

Hydroxycinnamic Acid antioxidants: an electrochemical overview.  

PubMed

Hydroxycinnamic acids (such as ferulic, caffeic, sinapic, and p-coumaric acids) are a group of compounds highly abundant in food that may account for about one-third of the phenolic compounds in our diet. Hydroxycinnamic acids have gained an increasing interest in health because they are known to be potent antioxidants. These compounds have been described as chain-breaking antioxidants acting through radical scavenging activity, that is related to their hydrogen or electron donating capacity and to the ability to delocalize/stabilize the resulting phenoxyl radical within their structure. The free radical scavenger ability of antioxidants can be predicted from standard one-electron potentials. Thus, voltammetric methods have often been applied to characterize a diversity of natural and synthetic antioxidants essentially to get an insight into their mechanism and also as an important tool for the rational design of new and potent antioxidants. The structure-property-activity relationships (SPARs) correlations already established for this type of compounds suggest that redox potentials could be considered a good measure of antioxidant activity and an accurate guideline on the drug discovery and development process. Due to its magnitude in the antioxidant field, the electrochemistry of hydroxycinnamic acid-based antioxidants is reviewed highlighting the structure-property-activity relationships (SPARs) obtained so far. PMID:23956973

Teixeira, José; Gaspar, Alexandra; Garrido, E Manuela; Garrido, Jorge; Borges, Fernanda

2013-07-16

330

Hydroxycinnamic Acid Antioxidants: An Electrochemical Overview  

PubMed Central

Hydroxycinnamic acids (such as ferulic, caffeic, sinapic, and p-coumaric acids) are a group of compounds highly abundant in food that may account for about one-third of the phenolic compounds in our diet. Hydroxycinnamic acids have gained an increasing interest in health because they are known to be potent antioxidants. These compounds have been described as chain-breaking antioxidants acting through radical scavenging activity, that is related to their hydrogen or electron donating capacity and to the ability to delocalize/stabilize the resulting phenoxyl radical within their structure. The free radical scavenger ability of antioxidants can be predicted from standard one-electron potentials. Thus, voltammetric methods have often been applied to characterize a diversity of natural and synthetic antioxidants essentially to get an insight into their mechanism and also as an important tool for the rational design of new and potent antioxidants. The structure-property-activity relationships (SPARs) correlations already established for this type of compounds suggest that redox potentials could be considered a good measure of antioxidant activity and an accurate guideline on the drug discovery and development process. Due to its magnitude in the antioxidant field, the electrochemistry of hydroxycinnamic acid-based antioxidants is reviewed highlighting the structure-property-activity relationships (SPARs) obtained so far.

Teixeira, Jose; Gaspar, Alexandra; Garrido, E. Manuela; Garrido, Jorge; Borges, Fernanda

2013-01-01

331

Essential oils content and antioxidant properties of peel ethanol extract in 18 lemon cultivars  

Microsoft Academic Search

Composition of essential oils and antioxidant activity of peel ethanol extract were analyzed in 18 local lemon cultivars. Essential oils composition was determined by GC\\/FID analysis, and antioxidant activity with the ABTS method. Fruit weight, polar and equatorial diameters, peel thickness, seed number, juice percentage, titratable acidity and juice pH were also determined for each cultivar. The main component in

C. Di Vaio; G. Graziani; A. Gaspari; G. Scaglione; S. Nocerino; A. Ritieni

2010-01-01

332

Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia  

Microsoft Academic Search

The aim of the present work was to investigate and compare phenolic compounds and antioxidant activity of methanol extracts of Quercus robur and Quercus cerris acorn kernels obtained before and after thermal treatment. Content of total phenolics, tannins, non-tannin phenolics and flavonoids was determined spectrophotometrically and content of gallic acid with HPLC. Antioxidant activity of the samples was assayed through

Sveto Raki?; Silvana Petrovi?; Jelena Kuki?; Milka Jadranin; Vele Teševi?; Dragan Povrenovi?; Slavica Šiler-Marinkovi?

2007-01-01

333

Prooxidant and antioxidant properties of human serum ultrafiltrates toward LDL: important role of uric acid  

Microsoft Academic Search

Oxidized LDL is present within atherosclerotic lesions, demonstrating a failure of antioxidant protection. A normal human serum ultrafiltrate of M r below 500 was prepared as a model for the low M r components of intersti- tial fluid, and its effects on LDL oxidation were investi- gated. The ultrafiltrate (0.3%, v\\/v) was a potent antioxidant for native LDL, but was

Rebecca A. Patterson; Elizabeth T. M. Horsley; David S. Leake

2003-01-01

334

Antioxidant properties of different fractions of tubers from Pueraria tuberosa Linn  

Microsoft Academic Search

Pueraria tuberosa Linn. (PT), Leguminosae, is a perennial climber, growing throughout tropical parts of India. In the Ayurvedic system of medicine, it is used as a drug of choice to manage pain, inflammation and other related diseases. The antioxidant potency of P. tuberosa was investigated for the first time. Total antioxidant capacity was determined using an ABTS?+ assay. Lipid peroxidation

Nidhi Pandey; J. K. Chaurasia; O. P. Tiwari; Yamini B. Tripathi

2007-01-01

335

Antioxidant property of polyhydroxylated naphthoquinone pigments from shells of purple sea urchin Anthocidaris crassispina  

Microsoft Academic Search

Sea urchin gonads are highly priced sushi foodstuff “Uni” in Japanese traditional food, but after removal of them the residual shells with spines are dumped as waste. However, sea urchin shells contain naphthoquinone pigments with several phenolic hydroxyl groups, which were expected to act as potent antioxidant substances by donating hydrogens. Our previous study has evaluated their antioxidant ability to

Rui Kuwahara; Hideo Hatate; Tamami Yuki; Hisashi Murata; Ryusuke Tanaka; Yoichiro Hama

2009-01-01

336

Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract  

Microsoft Academic Search

Pomegranate is an important source of bioactive compounds and has been used for folk medicine for many centuries. Pomegranate juice has been demonstrated to be high in antioxidant activity and is effective in the prevention of atherosclerosis. In a previous study, we found that pomegranate peel had the highest antioxidant activity among the peel, pulp and seed fractions of 28

Yunfeng Li; Changjiang Guo; Jijun Yang; Jingyu Wei; Jing Xu; Shuang Cheng

2006-01-01

337

Antioxidant properties of blackberry and blueberry fruits grown in the Black Sea Region of Turkey  

Microsoft Academic Search

Seven wild and ten cultivated blackberries (Arapaho, Bartin, Black Satin, Bursa 1, Bursa 2, Cherokee, Chester, Jumbo, Navaho, and Ness), and six lowbush (Vaccinium arctostaphylos) and four highbush (Vaccinium corymbosum) blueberries fruits (Ivanhoe, Jersey, Northland, and Rekord) were analyzed for total anthocyanins, total phenolics, and antioxidant activity as ferric reducing antioxidant power (FRAP) in this study. The respective ranges of

Ilkay Koca; Bulent Karadeniz

2009-01-01

338

Antioxidant and emulsifying properties of alcalase-hydrolyzed potato proteins in meat emulsions with different fat concentrations.  

PubMed

The effect of hydrolyzed potato protein (HPP), a natural antioxidant, on emulsion quality was investigated using a factorial design with two Fat (15%, 30%) and two HPP (0%, 2.5%) levels, with three replications. The colour of the raw emulsions as well as cooking losses, textural properties and TBARS of cooked frankfurters were measured. Increasing the Fat proportion significantly (P<0.05) increased L(?), H(ab)(0) and decreased a(?), b(?), C(ab)(?) and hardness. Meat emulsions with added HPP were darker (lower L(?)) than those made without HPP and also had lower values of a(?) and b(?). The addition of HPP (2.5%) significantly (P<0.05) decreased cooking losses and fracture force, and had a significant (P<0.05) inhibitory effect on lipid oxidation in cooked frankfurters. These results suggest that HPP has both antioxidant and emulsifying properties which may be of potential use in meat emulsion manufacturing. PMID:20416748

Nieto, Gema; Castillo, Manuel; Xiong, Youling L; Alvarez, Daniel; Payne, Fred A; Garrido, María Dolores

2009-03-26

339

Antioxidant properties, phytochemical composition, and antiproliferative activity of Maryland-grown soybeans with colored seed coats.  

PubMed

This study characterized and compared 18 colored seed coat soybeans for the isoflavone, total phenolic, and cyanidin-3-glucoside (Cy-3-glc) contents of their flour extracts and the fatty acid composition and carotenoid and alpha-tocopherol contents of their oils. Antioxidant assays also assessed activity of the flour extracts against peroxyl, hydroxyl, and ABTS(+) radicals. Black seed coat soybeans had the highest TPC, ORAC, HOSC, and ABTS(+) radical scavenging values, in addition to the highest isoflavone content, and were the only color to contain Cy-3-glc. Five soybeans (two black and one each brown, yellow, and green) were selected to test their effects on HT-29 human colorectal cancer cell growth. The effects of the hydrolyzed and unhydrolyzed extracts were compared to an aglycone isoflavone standard mixture of the same total molar concentration as the highest soybean concentration of 15 mg of seed flour equiv/mL of treatment media. All high doses of hydrolyzed soybean treatments except the green genotype significantly reduced cell number compared to control at 3 h of treatment time, whereas the high dose of isoflavone standard treatment took 72 h to show a significant reduction (P < 0.05). PMID:19950996

Slavin, Margaret; Kenworthy, William; Yu, Liangli Lucy

2009-12-01

340

Facile chemiluminescence assay for antioxidative properties of vegetable lipids: fundamentals and illustrative examples.  

PubMed

The general approach disclosed herein opens the new possibilities of exploiting the oxidation processes followed by chemiluminescence (CL) emission for the assessment of an antioxidant potential of natural lipid materials and enables determination of the amount and strength of lipid-borne antioxidants in one experiment. The reliability of the analytical procedure is completely unaffected by an inevitable entering of oxidizable lipid portions into the probe chemiluminescent mixture, which is exemplarily illustrated for the case of vegetable oils which served as sources of antioxidant-containing lipids. As a matter of fact, the difference in the effective radical-scavenging rate constants, determined for the antioxidative constituents of the sunflower and corn oils, perfectly matches the distinction of their qualitative tocopherol contents. In addition to the antiradical activity of lipid samples, the antioxidant potential of the latter may be modified by their influence on hydroperoxide stability, as it has been also demonstrated in the present work. PMID:19768225

Fedorova, Galina F; Menshov, Valery A; Trofimov, Alexey V; Vasil'ev, Rostislav F

2009-08-17

341

Unique Properties of Polyphenol Stilbenes in the Brain: More than Direct Antioxidant Actions; Gene\\/Protein Regulatory Activity  

Microsoft Academic Search

The ‘French Paradox’ has been typically associated with moderate consumption of wine, especially red wine. A polyphenol 3,4’,5-trihydroxy-trans-stilbene (a member of the non-flavonoids family), better known as resveratrol, has been purported to have many health benefits. A number of these valuable properties have been attributed to its intrinsic antioxidant capabilities, although the potential level of resveratrol in the circulation is

Sylvain Doré

2005-01-01

342

Influence of degree of hydrolysis on functional properties, antioxidant activity and ACE inhibitory activity of peanut protein hydrolysate  

Microsoft Academic Search

Functional properties, antioxidant and Angiotensin-converting enzyme (ACE) inhibitory activities of peanut protein isolate (PPI) and peanut protein hydrolysate (PPH) prepared using Alcalase, at different (10%, 20%, 30% and 40%) degrees of hydrolysis, (DH) were investigated. Hydrolysis (at DH>10%) significantly (p<0.05) improved the solubility (>80%) of PPI, especially in the pH range of 4–6. However, PPI showed better emulsifying and foaming

S. N. Jamdar; V. Rajalakshmi; M. D. Pednekar; F. Juan; V. Yardi; Arun Sharma

2010-01-01

343

Antioxidant properties and cytotoxic effects of fractions from glasswort ( Salicornia herbacea ) seed extracts on human intestinal cells  

Microsoft Academic Search

Glasswort (Salicornia herbacea L.) is a halophyte that grows in the salt marshes and has been used as a seasoned vegetable and a traditional folk medicine.\\u000a In the present study, the antioxidant properties and cytotoxic effects of glasswort seeds on human colon and intestinal cells\\u000a were investigated. A series of solvent fractions, including fractions of hexane (Fr.H), ethyl ether (Fr.E),

Smee Kang; Daram Kim; Bo Hyun Lee; Mi-Ri Kim; Maehee Chiang; Jungil Hong

2011-01-01

344

Dopaminergic pharmacology and antioxidant properties of pukateine, a natural product lead for the design of agents increasing dopamine neurotransmission  

Microsoft Academic Search

The dopaminergic and antioxidant properties of pukateine [(R)-11-hydroxy-1,2-methylenedioxyaporphine, PUK], a natural aporphine derivative, were analyzed in the rat central nervous system. At dopamine (DA) D1 ([3H]-SCH 23390) and D2 ([3H]-raclopride) binding sites, PUK showed IC50 values in the submicromolar range (0.4 and 0.6 ?M, respectively). When the uptake of tritiated dopamine was assayed by using a synaptosomal preparation, PUK showed

Federico A Dajas-Bailador; Marcelo Asencio; Carolina Bonilla; Ma. Cecilia Scorza; Carolina Echeverry; Miguel Reyes-Parada; Rodolfo Silveira; Philippe Protais; Graeme Russell; Bruce K Cassels; Federico Dajas

1999-01-01

345

ANTIOXIDANT PROPERTIES OF GREEK O. DICTAMNUS AND R. OFFICINALIS METHANOL AND AQUEOUS EXTRACTS - HPLC DETERMINATION OF PHENOLIC ACIDS  

Microsoft Academic Search

This study determined the total phenol (TP), phenolic acid (PA) content as well as the free radical scavenging activity (RSA) and ferric reducing antioxidant properties (FRAP) of methanol and aqueous extracts of R.officinalis and O.dictamnus species (Crete, Greece). The methanol extracts showed higher RSA (P<0.05) than the aqueous extracts (mean EC50 value: 0.33 and 0.38mg\\/mL, resp.). Rosemary was superior in

V. Lagouri; G. Alexandri

2011-01-01

346

New aspects of the antioxidant properties of phenolic acids: a combined theoretical and experimental approach.  

PubMed

Ferulic acid is widely distributed in the leaves and seeds of cereals as well as in coffee, apples, artichokes, peanuts, oranges and pineapples. Like numerous other natural polyphenols it exhibits antioxidant properties. It is known to act as a free radical scavenger by H atom transfer from the phenolic OH group. In the present joint experimental and theoretical studies we studied a new mechanism to explain such activities. Ferulic acid can indeed act by radical addition on the alpha,beta-double bond. On the basis of the identification of metabolites formed in an oxidative radiolytic solution and after DFT calculations, we studied the thermodynamic and kinetic aspects of this reaction. Addition and HAT reactions were treated as competitive reactions. The possibility of dimer formation was also investigated from a theoretical point of view; the high barriers we obtained contribute to explaining why we did not observe those compounds as major radiolytic compounds. The DPPH free radical scavenging capacity of ferulic acid and the oxidative products was measured and is discussed on the basis of DFT calculations (BDEs and spin densities). PMID:19950505

Anouar, E; Kosinová, P; Kozlowski, D; Mokrini, R; Duroux, J L; Trouillas, P

2009-09-21

347

Effects of ionizing radiation on microbial decontamination, phenolic contents, and antioxidant properties of triphala.  

PubMed

Triphala, a mixture of Emblica officinalis, Terminalia chebula, and Terminalia bellirica, containing ingredients from plant origin, is often prone to microbial contamination. A high level of microbial contamination was observed in Triphala samples obtained from different sources. On gamma radiation processing, a sharp decline in log CFU was observed with increasing radiation dose and a complete decontamination at 5 kGy. Average D10 value for total aerobic and fungal counts were observed to be 0.55 +/- 0.073 kGy and 0.94 +/- 0.043 kGy, respectively. Water extracts of irradiated samples showed linearly increasing concentration of gallic acid (3.3 to 4.5 times), total phenolic contents (2.16 to 2.87 times), and antioxidant properties with increasing radiation dose up to 25 kGy. The increase could be attributed to easy release of active ingredients from their radiation degraded complex forms. Aflatoxin B(1) and ochratoxin could not be detected in the samples. Gamma-radiation dose up to 5 kGy could be safely used to hygienize Triphala. PMID:19397725

Kumari, N; Kumar, P; Mitra, D; Prasad, B; Tiwary, B N; Varshney, L

2009-04-01

348

Antioxidant properties of aqueous extracts from selected lamiaceae species grown in Turkey.  

PubMed

Water-soluble extracts from black thyme (Thymbra spicata L.), savory (Satureja cuneifolia Ten.), Spanish oregano (Coridothymus capitatus (L.) Reichb. f.), sweet marjoram (Majorana hortensis Moench), Syrian oregano (Origanum syriacum L.), Toka oregano (Origanum minutiflorum O. Schwarz et P. H. Davis), and Turkish oregano (Origanum onites L.) were screened for antioxidant properties in a battery of six in vitro assays. Total phenol content and qualitative-quantitative compositional analyses were also carried out. The extracts demonstrated varying degrees of efficacy in each screen. The savory extract was the most effective at reducing iron(III), scavenging 1,1-diphenyl-2-picrylhydrazyl radicals, inhibiting ascorbate-iron(III)-catalyzed hydroxyl radical-mediated brain phospholipid peroxidation, and site-specific hydroxyl radical-mediated 2-deoxy-d-ribose degradation. The Syrian oregano extract was the most effective chelator of iron(II), while Spanish and Turkish oregano extracts were the most effective inhibitors of nonsite-specific hydroxyl radical-mediated 2-deoxy-d-ribose degradation. All the extracts contained Folin-Ciocalteu reagent-reactive substances, which was confirmed by the presence of polar phenolic analytes (i.e., hydroxybenzoates, hydroxycinnamates, and flavonoids). PMID:14969528

Dorman, H J Damien; Bachmayer, Oliver; Kosar, Müberra; Hiltunen, Raimo

2004-02-25

349

Dipeptidyl peptidase IV inhibitory and antioxidative properties of milk protein-derived dipeptides and hydrolysates.  

PubMed

Selected synthetic dipeptides and milk protein hydrolysates were evaluated for their dipeptidyl peptidase IV (DPP-IV) inhibitory properties, and their superoxide (SO) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. DPP-IV inhibition was seen with eight out of the twelve dipeptides and 5 of the twelve hydrolysates studied. Trp-Val inhibited DPP-IV, however, inhibition was not observed with the reverse peptide Val-Trp. The most potent hydrolysate inhibitors were generated from casein (CasH2) and lactoferrin (LFH1). Two Trp containing dipeptides, Trp-Val and Val-Trp, and three lactoferrin hydrolysates scavenged DPPH. The dipeptides had higher SO EC(50) values compared to the milk protein hydrolysates (arising from three lactoferrin and one whey protein hydrolysates). Higher molecular mass fractions of the milk protein hydrolysates were associated with the SO scavenging activity. Trp-Val and one lactoferrin hydrolysate (LFH1) were multifunctional displaying both DPP-IV inhibitory and antioxidant (SO and DPPH scavenging) activities. These compounds may have potential as dietary ingredients in the management of type 2 diabetes by virtue of their ability to scavenge reactive oxygen species and to extend the half-life of incretin molecules. PMID:23219487

Nongonierma, Alice B; FitzGerald, Richard J

2012-12-03

350

Endothelium-dependent vasodilator and antioxidant properties of a novel enzymatic extract of grape pomace from wine industrial waste.  

PubMed

The aim of the present study was to evaluate the vascular effects of an enzymatic extract of grape pomace (GP-EE) on isolated arteries, focusing our attention on endothelium-derived relaxation and on its antioxidant properties. Grape pomace derived from wine making was extracted by an enzymatic process and its composition of polyphenols was evaluated by HPLC and ESI-MS/MS, detecting kaempferol, catechin, quercetin and procyanidins B1 and B2, trace levels of resveratrol and tracing out gallocatechin and anthocyanidins. GP-EE induced endothelium- and NO-dependent vasodilatation of both rat aorta and small mesenteric artery (SMA) segments and reduced Phe-induced response in aortic rings. Both ORAC and DPPH assays confirmed antioxidant scavenging properties of GP-EE, which also prevented O(2)(·-) production (assessed by DHE fluorescence) and contraction elicited by ET-1. These results provide evidence that GP-EE possesses interesting antioxidant and protective vascular properties and highlight the potential interest of this extract as a functional food. PMID:22953822

Rodriguez-Rodriguez, Rosalia; Justo, Maria Luisa; Claro, Carmen Maria; Vila, Elisabet; Parrado, Juan; Herrera, Maria Dolores; Alvarez de Sotomayor, Maria

2012-05-30

351

Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extracts  

Microsoft Academic Search

We have investigated the antioxidant properties (FRAP ferric-reducing ability and ABTS radical-scavenging capacity) as well as the light barrier properties of gelatin-based edible films containing oregano or rosemary aqueous extracts. For comparative purposes both bovine-hide and tuna-skin gelatins were studied. The oregano and rosemary extracts were first characterised by both their total phenolic content and antioxidant activity, with the oregano

J. Gómez-Estaca; L. Bravo; M. C. Gómez-Guillén; A. Alemán; P. Montero

2009-01-01

352

Improvement of quality and antioxidant properties of dried mulberry leaves with combined far-infrared radiation and air convection in Thai tea process  

Microsoft Academic Search

Combined far-infrared radiation with hot-air convection (FIR–HA) drying was used for improving colour and antioxidant properties of mulberry leaf tea. Antioxidant properties and phenolic compounds of FIR–HA dried mulberry tea were determined and compared with the commercial product and with fresh leaves. We found that a smaller decrease in L and b values of the FIR–HA dried tea than those

Pitchaporn Wanyo; Sirithon Siriamornpun; Naret Meeso

2011-01-01

353

Isolation and characterization of antioxidant and antibacterial compound from mango ginger ( Curcuma amada Roxb.) rhizome  

Microsoft Academic Search

The chloroform extract of mango ginger (Curcuma amada Roxb.) rhizome was subjected to antioxidant activity-guided purification by repeated silica gel column chromatography to obtain a pure antioxidant compound. The structure was deduced by analyzing UV, IR, liquid chromatography–mass spectrometry (LC–MS) and two-dimensional heteronuclear multiple quantum coherence transfer spectroscopy (2D-HMQCT) NMR spectral data, and named it as “Amadannulen”, a novel compound.

R. S. Policegoudra; K. Abiraj; D. Channe Gowda; S. M. Aradhya

2007-01-01

354

Oxidative stress as a risk factor for osteoporosis in elderly Mexicans as characterized by antioxidant enzymes  

Microsoft Academic Search

BACKGROUND: Oxidative stress (OxS) has recently been linked with osteoporosis; however, we do not know the influence of OxS as an independent risk factor for this disease. METHODS: We conducted a case-control study in 94 subjects ?60 years of age, 50 healthy and 44 with osteoporosis. We measured total antioxidant status, plasma lipid peroxides, antioxidant activity of superoxide dismutase and

Martha A Sánchez-Rodríguez; Mirna Ruiz-Ramos; Elsa Correa-Muñoz; Víctor Manuel Mendoza-Núñez

2007-01-01

355

Assessment of Antioxidant Properties in Fruits of Myrica esculenta: A Popular Wild Edible Species in Indian Himalayan Region  

PubMed Central

Crude extract of Myrica esculenta fruits, a wild edible species of Indian Himalayan Region, was evaluated for phenolic compounds and antioxidant properties. Results revealed significant variation in total phenolic and flavonoid contents across populations. Among populations, total phenolic content varied between 1.78 and 2.51?mg gallic acid equivalent/g fresh weight (fw) of fruits and total flavonoids ranged between 1.31 and 1.59?mg quercetin equivalent/g fw. Antioxidant activity determined by 2,2?-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, 1,1-diphenyl-2-picrylhydrazyl radical scavenging and ferric reducing antioxidant power (FRAP) exhibited considerable antioxidant potential and showed significant positive correlation with total phenolic and total flavonoids content. High performance liquid chromatography analysis revealed significant variation (P

Rawat, Sandeep; Jugran, Arun; Giri, Lalit; Bhatt, Indra D.; Rawal, Ranbeer S.

2011-01-01

356

Antioxidant and antimutagenic properties of the monoterpene indole alkaloid psychollatine and the crude foliar extract of Psychotria umbellata Vell.  

PubMed

Psychollatine is a monoterpene indole alkaloid produced and accumulated by Psychotria umbellata Vell. (Rubiaceae) leaves in relatively high amounts (approximately 3% of the dry weight). The alkaloid has been shown to display opioid-like analgesic, anxiolytic, antidepressive and antipsychotic activities in rodents. In vitro assays suggested a protective role for this molecule in plant oxidative stress responses. This work reports antioxidant properties of psychollatine and the crude foliar extract from P. umbellata in strains of Saccharomyces cerevisiae proficient and deficient in antioxidant defenses exposed to H2O2 and paraquat. The antimutagenic activity of P. umbellata and its main alkaloid were assayed in S. cerevisiae N123 strain in presence of H2O2. Moreover, the antioxidant capacity of these substances on the hydroxyl radical (OH.) was investigated, using the hypoxanthine/xanthine oxidase assay. Psychollatine and the crude foliar extract of P. umbellata showed protective effect against oxidative stress in yeast, acting both as antioxidant and antimutagenic agents. PMID:18093795

Fragoso, Variluska; do Nascimento, Naíla Cannes; Moura, Dinara Jaqueline; e Silva, Ana Catarina Romano; Richter, Marc François; Saffi, Jenifer; Fett-Neto, Arthur Germano

2007-11-19

357

Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents.  

PubMed

Total equivalent antioxidant capacity (TEAC) and phenolic content of 26 common spice extracts from 12 botanical families were investigated. Qualitative and quantitative analyses of major phenolics in the spice extracts were systematically conducted by reversed-phase high-performance liquid chromatography (RP-HPLC). Many spices contained high levels of phenolics and demonstrated high antioxidant capacity. Wide variation in TEAC values (0.55-168.7 mmol/100 g) and total phenolic content (0.04-14.38 g of gallic acid equivalent/100 g) was observed. A highly positive linear relationship (R2= 0.95) obtained between TEAC values and total phenolic content showed that phenolic compounds in the tested spices contributed significantly to their antioxidant capacity. Major types of phenolic constituents identified in the spice extracts were phenolic acids, phenolic diterpenes, flavonoids, and volatile oils (e.g., aromatic compounds). Rosmarinic acid was the dominant phenolic compound in the six spices of the family Labiatae. Phenolic volatile oils were the principal active ingredients in most spices. The spices and related families with the highest antioxidant capacity were screened, e.g., clove in the Myrtaceae, cinnamon in the Lauraceae, oregano in the Labiatae, etc., representing potential sources of potent natural antioxidants for commercial exploitation. This study provides direct comparative data on antioxidant capacity and total and individual phenolics contents of the 26 spice extracts. PMID:16190627

Shan, Bin; Cai, Yizhong Z; Sun, Mei; Corke, Harold

2005-10-01

358

Heptacoordinate tin(IV) compounds derived from pyridine Schiff bases: synthesis, characterization, in vitro cytotoxicity, anti-inflammatory and antioxidant activity.  

PubMed

Tin(IV) complexes 2a--q derived from pyridine Schiff bases were prepared and characterized. Four complexes of this series were evaluated in vitro against different carcinogenic cell lines; besides their anti-inflammatory and antioxidant properties were also tested. Combination of mass spectrometry, multinuclear NMR and X-ray diffraction techniques evidenced the formation of heptacoordinated monomeric species. The X-ray diffraction analysis of 2a, 2b, 2i, 2j and 2n led to establish the heptacoordination around the tin atom in solid state and also revealed that the ligand occupies the equatorial positions of the distorted pentagonal bipyramidal geometry and the two alkyl or aryl groups the axial positions. The in-vitro study for complexes 2a--d against six tumor cell lines showed varied antiproliferative activity, the IC50 for all tested complexes was lower than that of the cis-platin. Compounds 2a--d also exhibited anti-inflammatory activity where complex 2c resulted to be more active (IC50=0.11 microM) than the indomethacin IC50=0.27 microM which was used as reference. The antioxidant activity in rat brain homogenate on inhibition of thiobarbituric acid reactive substances (TBARS) indicated that 2c (IC50=1.77 microM) is more active than the quercetine (4.11 microM) and alpha-tocopherol (IC50=569.09 microM). PMID:19122310

González, Arturo; Gómez, Elizabeth; Cortés-Lozada, Armando; Hernández, Simón; Ramírez-Apan, Teresa; Nieto-Camacho, Antonio

2009-01-01

359

An Investigation into the Physico-chemical Properties of Transformer Oil Blends with Antioxidants extracted from Turmeric Powder  

NASA Astrophysics Data System (ADS)

The blending of transformer oil (used mainly as an insulating oil) with appropriate synthetic antioxidants, such as BHT (2,6-di-tert-butyl-4-methylphenol) and DBP (2,6-di-tert-butylphenol) have been previously reported. This article is focused on the use of antioxidant extracts from turmeric (Curcuma longa), a natural source. Turmeric is well known for its antimicrobial, antioxidant and anticarcinogenic properties owing to the active nature of its components. Extracts from powdered turmeric were subsequently blended into naphthenic-based uninhibited virgin transformer oil, hereinafter referred to as extract-oil blends (E-OB). Thin-layer chromatography (TLC) of the oil blends revealed that five components extracted from turmeric powder were successfully blended into the oil. Subsequent gas chromatography-mass spectrometry (GC-MS) analysis confirmed the presence of the compounds: curcumene, sesquiphellandrene, ar-turmerone, turmerone and curlone. Thermogravimetric analysis (TGA) of the extract-oil blends, containing various levels of extracts, revealed an average temperature shift of ˜8.21°C in the initial onset of degradation in comparison to virgin non-blended oil. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that an increase in the mass aliquot of turmeric extracts in the transformer oil increased the free radical scavenging activity of the oil. Electrical properties of the oil investigated showed that the dissipation factor in the blended oil was found to be lower than that of virgin transformer oil. Evidently, a lower dissipation value renders the oil blend as a superior insulator over normal virgin non-blended oil. This investigation elucidated improved physico-chemical properties of transformer oil blended with turmeric antioxidant extracts.

Dukhi, Veresha; Bissessur, Ajay; Ngila, Catherine Jane; Ijumba, Nelson Mutatina

2013-07-01

360

Synthesis, characterization and DNA-binding properties of Ln(III) complexes with 6-ethoxy chromone-3-carbaldehyde benzoyl hydrazone.  

PubMed

A novel 6-ethoxy chromone-3-carbaldehyde benzoyl hydrazone (L) and its Ln(III) complexes, [Ln = Sm (1), Eu (2), Gd (3), Tb (4)], have been synthesized and characterized. The fluorescence properties of the Eu(III) and Sm(III) complexes in solid state and Eu(III) complex in different solutions (DMF, DMSO, methanol and acetonitrile) were investigated. At the same time, the DNA-binding properties of the two complexes are investigated using UV-Vis absorption spectroscopy, fluorescence spectroscopy, viscosity measurement. All the experimental evidences indicate that the two complexes can bind to CT-DNA via an intercalation mechanism. Furthermore, antioxidant activity tests in vitro showed that the complexes have significant antioxidative activity against hydroxyl free radicals from the Fenton reaction. PMID:19370403

Wang, Ju; Yang, Zheng-Yin; Wang, Bao-Dui; Yi, Xu-Yang; Liu, Yong-Chun

2009-04-16

361

Oxidant, antioxidant and physical exercise  

Microsoft Academic Search

Generation of reactive oxygen species (ROS) is a normal process in the life of aerobic organisms. Under physiological conditions, these deleterious species are mostly removed by the cellular antioxidant systems, which include antioxidant vitamins, protein and non-protein thiols, and antioxidant enzymes. Since the antioxidant reserve capacity in most tissues is rather marginal, strenuous physical exercise characterized by a remarkable increase

Alok K. Banerjee; Amritlal Mandal; Dipanjan Chanda; Sajal Chakraborti

2003-01-01

362

In Vivo Biochemical and Gene Expression Analyses of the Antioxidant Activities and Hypocholesterolaemic Properties of Tamarindus indica Fruit Pulp Extract  

PubMed Central

Background Tamarindus indica (T. indica) is a medicinal plant with many biological activities including anti-diabetic, hypolipidaemic and anti-bacterial activities. A recent study demonstrated the hypolipidaemic effect of T. indica fruit pulp in hamsters. However, the biochemical and molecular mechanisms responsible for these effects have not been fully elucidated. Hence, the aims of this study were to evaluate the antioxidant activities and potential hypocholesterolaemic properties of T. indica, using in vitro and in vivo approaches. Methodology/Principal Findings The in vitro study demonstrated that T. indica fruit pulp had significant amount of phenolic (244.9±10.1 mg GAE/extract) and flavonoid (93.9±2.6 mg RE/g extract) content and possessed antioxidant activities. In the in vivo study, hamsters fed with high-cholesterol diet for ten weeks showed elevated serum triglyceride, total cholesterol, HDL-C and LDL-C levels. Administration of T. indica fruit pulp to hypercholesterolaemic hamsters significantly lowered serum triglyceride, total cholesterol and LDL-C levels but had no effect on the HDL-C level. The lipid-lowering effect was accompanied with significant increase in the expression of Apo A1, Abcg5 and LDL receptor genes and significant decrease in the expression of HMG-CoA reductase and Mtp genes. Administration of T. indica fruit pulp to hypercholesterolaemic hamsters also protected against oxidative damage by increasing hepatic antioxidant enzymes, antioxidant activities and preventing hepatic lipid peroxidation. Conclusion/Significance It is postulated that tamarind fruit pulp exerts its hypocholesterolaemic effect by increasing cholesterol efflux, enhancing LDL-C uptake and clearance, suppressing triglyceride accumulation and inhibiting cholesterol biosynthesis. T. indica fruit pulp has potential antioxidative effects and is potentially protective against diet-induced hypercholesterolaemia.

Lim, Chor Yin; Mat Junit, Sarni; Abdulla, Mahmood Ameen; Abdul Aziz, Azlina

2013-01-01

363

Evaluation of antioxidant properties of Mallotus oppositifolium in in-vitro, in-vivo and ex-vivo model systems.  

PubMed

The protective effect of antioxidants and naturally occurring substances against oxidative stress damage has recently attracted much attention. The leaves of Mallotus oppositifolium, a shrub of the family Euphorbiacea that grows in many parts of Africa, are used in folk medicine and herbal preparations for the treatment of dysentery, worms and malaria. The study investigated the antioxidant properties of the methanolic extract of the leaves of Mallotus oppositifolium (MEMO) in comparison with butylated hydroxyl anisole (BHA) as a standard antioxidant using three free radical generators viz hydrophilic radical generator 2,2-azobis(2-amidino propane) dihydrochloride (AAPH), hydrophobic radical generator 2,2-azobis(2,4-dimethylvaleronitrile) (AMVN) and hydroxyl radical and non-specific radical generator Fe2+/ascorbate system in an in vitro, in vivo and ex-vivo model systems. Phytochemical analysis of the leaves extract was also assessed. Phytochemical analysis of the powdered leaves revealed the presence of alkaloids, tannins, cardenolides and saponins. In vitro study indicated that while MEMO failed to inhibit lipid peroxidation (LPO) induced by AAPH, while BHA offered 55.5% inhibition. In addition, while AMVN-induced LPO was inhibited by 17.7% and 29.4% by MEMO and BHA respectively, Fe2+/ascorbate system-induced LPO was inhibited by 57.9% and 78.9% by MEMO and BHArespectively. Ex-vivo studies showed that MEMO at 100mg/kg bw reduced malondialdehyde and protein carbonyl levels by 34.5% and 12.0% respectively compared with the control. In vivo, MEMO increased (P<0.05) superoxide dismutase and catalase activities by 408.0% and 295.0% respectively. Taken together, this study demonstrates that MEMO exhibits antioxidant, radical scavenging and enhancement of enzymatic antioxidant capacity and as such could intervene in toxicological processes mediated by free radical mechanisms. PMID:22416646

Adedara, I A; Adesanoye, O A; Farombi, E O

2010-12-01

364

Total phenolic content and antioxidative properties of commercial tinctures obtained from some Lamiaceae plants.  

PubMed

The antioxidant level of commercial tinctures from three Lamiaceae plants, Salvia officinalis, Mentha piperita, and Melissa officinalis, have been determined by the Folin-Ciocalteu method, the 2.2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging technique and ABTS assay. Total phenolic content was expressed as GAE (gallic acid equivalent) and ranged from 0.24 to 3.99 mg/mL. Antioxidant activity in the ABTS assay, calculated as TEAC (Trolox equivalent antioxidant capacity), ranged from 23.5 to 35.6 micromol Trolox/mL, while in the DPPH method, the EC50 value ranged from 0.04 to 0.07 mL/assay. Radical scavenging activity was correlated with total phenolic content. Correlations between ABTS and F-C methods, DPPH and F-C methods and ABTS and DPPH methods were calculated. The obtained results can be useful as additional information about the antioxidant activity of galenical preparations. PMID:23413570

Kowalczyk, Adam; Biskup, Izabela; Fecka, Izabela

2012-12-01

365

Antioxidant and iron-binding properties of curcumin, capsaicin, and S-allylcysteine reduce oxidative stress in rat brain homogenate.  

PubMed

Research demonstrates that antioxidants and metal chelators may be of beneficial use in the treatment of neurodegenerative diseases, such as Alzheimer's disease (AD). This study investigated the antioxidant and metal-binding properties of curcumin, capsaicin, and S-allylcysteine, which are major components found in commonly used dietary spice ingredients turmeric, chilli, and garlic, respectively. The DPPH assay demonstrates that these compounds readily scavenge free radicals. These compounds significantly curtail iron- (Fe2+) and quinolinic acid (QA)-induced lipid peroxidation and potently scavenge the superoxide anion generated by 1 mM cyanide in rat brain homogenate. The ferrozine assay was used to measure the extent of Fe2+ chelation, and electrochemistry was employed to measure the Fe3+ binding activity of curcumin, capsaicin, and S-allylcysteine. Both assays demonstrate that these compounds bind Fe2+ and Fe3+ and prevent the redox cycling of iron, suggesting that this may be an additional method through which these agents reduce Fe2+-induced lipid peroxidation. This study demonstrates the antioxidant and metal-binding properties of these spice ingredients, and it is hereby postulate that these compounds have important implications in the prevention or treatment of neurodegenerative diseases such as AD. PMID:18422331

Dairam, Amichand; Fogel, Ronen; Daya, Santy; Limson, Janice L

2008-04-19

366

Production, characterization and antioxidant potential of protease from Streptomyces sp. MAB18 using poultry wastes.  

PubMed

Poultry waste is an abundant renewable source for the recovery of several value-added metabolites with potential industrial applications. This study describes the production of protease on poultry waste, with the subsequent use of the same poultry waste for the extraction of antioxidants. An extracellular protease-producing strain was isolated from Cuddalore coast, India, and identified as Streptomyces sp. MAB18. Its protease was purified 17.13-fold with 21.62% yield with a specific activity of 2398.36?U/mg and the molecular weight was estimated as 43?kDa. The enzyme was optimally active at pH 8-10 and temperature 50-60 ° C and it was most stable up to pH 12 and 6-12% of NaCl concentration. The enzyme activity was reduced when treated with Hg(2+), Pb(2+), and SDS and stimulated by Fe(2+), Mg(2+), Triton X-100, DMSO (dimethyl sulfoxide), sodium sulphite, and ?-mercaptoethanol. Furthermore, the antioxidant activities of protease were evaluated using in vitro antioxidant assays, such as DPPH radical-scavenging activity, O2 scavenging activity, NO scavenging activity, Fe(2+) chelating activity, and reducing power. The enzyme showed important antioxidant potential with an IC50 value of 78 ± 0.28?mg/mL. Results of the present study indicate that the poultry waste-derived protease may be useful as supplementary protein and antioxidant in the animal feed formulations. PMID:23991418

Manivasagan, Panchanathan; Venkatesan, Jayachandran; Sivakumar, Kannan; Kim, Se-Kwon

2013-08-07

367

Production, Characterization and Antioxidant Potential of Protease from Streptomyces sp. MAB18 Using Poultry Wastes  

PubMed Central

Poultry waste is an abundant renewable source for the recovery of several value-added metabolites with potential industrial applications. This study describes the production of protease on poultry waste, with the subsequent use of the same poultry waste for the extraction of antioxidants. An extracellular protease-producing strain was isolated from Cuddalore coast, India, and identified as Streptomyces sp. MAB18. Its protease was purified 17.13-fold with 21.62% yield with a specific activity of 2398.36?U/mg and the molecular weight was estimated as 43?kDa. The enzyme was optimally active at pH 8–10 and temperature 50–60°C and it was most stable up to pH 12 and 6–12% of NaCl concentration. The enzyme activity was reduced when treated with Hg2+, Pb2+, and SDS and stimulated by Fe2+, Mg2+, Triton X-100, DMSO (dimethyl sulfoxide), sodium sulphite, and ?-mercaptoethanol. Furthermore, the antioxidant activities of protease were evaluated using in vitro antioxidant assays, such as DPPH radical-scavenging activity, O2 scavenging activity, NO scavenging activity, Fe2+ chelating activity, and reducing power. The enzyme showed important antioxidant potential with an IC50 value of 78 ± 0.28?mg/mL. Results of the present study indicate that the poultry waste-derived protease may be useful as supplementary protein and antioxidant in the animal feed formulations.

Manivasagan, Panchanathan; Sivakumar, Kannan; Kim, Se-Kwon

2013-01-01

368

Evaluation of antioxidant properties of medical plants using microbial test systems  

Microsoft Academic Search

The antioxidant activities of extracts from leaves of the medicinal plants growing in Siberia were examined. Total antioxidant\\u000a activity was determined using in vitro methods including DPPH (2,2-diphenyl-1-picrylhydrazyl radical) free radical scavenging\\u000a assay, chelating capacity assay with ferrozine, evaluation of capacity to protect plasmid DNA against oxidative damage, measurement\\u000a of H2O2 production, and measurement of total flavonoid and tannin content

Galina V. Smirnova; Galina I. Vysochina; Nadezda G. Muzyka; Zoya Y. Samoylova; Tatyana A. Kukushkina; Oleg N. Oktyabrsky

2010-01-01

369

Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal  

Microsoft Academic Search

Pollen analysis, total phenols content and antioxidant activity were studied for the first time in Portuguese propolis samples from Bornes and Fundão regions. Total phenols content was determined by colorimetric assay and their amount was of 329mg\\/g of GAE in Bornes sample and 151mg\\/g of GAE in Fundão propolis. The antioxidant capacity of propolis extracts was assessed through the scavenging

Leandro Moreira; Luís G. Dias; José Alberto Pereira; Leticia Estevinho

2008-01-01

370

Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata  

Microsoft Academic Search

Antioxidant activity (AA), total phenolic content, and reducing power of the crude extract, fractions, and subfractions derived from a red alga, Polysiphonia urceolata, were evaluated and determined. The antioxidative activity was measured using the ?,?-diphenyl-?-picrylhydrazyl (DPPH) radical scavenging assay and the ?-carotene–linoleate assay systems, and compared with that of butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). The

Xiao-Juan Duan; Wei-Wei Zhang; Xiao-Ming Li; Bin-Gui Wang

2006-01-01

371

Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha)  

Microsoft Academic Search

Water and methanol extracts of persimmon leaf tea were studied for antioxidant and radical-scavenging activities. Antioxidant activity was measured using a ?-carotene bleaching method. The activity of water extract was very strong and 0.125% higher than that of 10 mM ascorbic acid. The scavenging activity against superoxide anion radicals by methanol extract was stronger than that of the water extract,

Senji Sakanaka; Yumi Tachibana; Yuki Okada

2005-01-01

372

Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions  

Microsoft Academic Search

Antioxidant and antimicrobial activities as well as the quantity of phenolic substances (total phenol, flavonoid and phenolic acid contents) were determined in aqueous extracts of leaves, stems and flowers of Moltkia petraea (Tratt.) Griseb. from two mountainous localities (Sveti Jure and Sniježnica) in Croatia. In addition, the profile of phenolic acids was analyzed by UPLC–MS\\/MS. Antioxidant activities of all extracts

M. Zovko Kon?i?; D. Kremer; J. Gruz; M. Strnad; G. Biševac; I. Kosalec; D. Šamec; J. Piljac-Žegarac; K. Karlovi?

2010-01-01

373

Styrylpyrones from the medicinal fungus Phellinus baumii and their antioxidant properties.  

PubMed

During the search for natural antioxidants from basidiomycetes, a new styrylpyrone, baumin (1), was isolated from the cultivated medicinal fungus Phellinus baumii, together with known compounds, davallialactone (2), hispidin (3), hypholomine B (4), interfungin A (5), inoscavin A (6), and phelligridin D (7), which were previously isolated from the medicinal fungi Phellinusignarius, Phellinuslinteus, and Inonotus xeranticus. Their structures were elucidated by extensive spectroscopic methods. These compounds exhibited antioxidant activity through Fenton reaction inhibition via iron chelation and free radical scavenging. PMID:20708931

Lee, In-Kyoung; Han, Myung-Suk; Lee, Myeong-Seok; Kim, Young-Sook; Yun, Bong-Sik

2010-07-25

374

Purification and characterization of antioxidant components from the fruiting bodies of Pleurotus abalonus including 9-beta-d-ribofuranosidoadenine, 5'-deoxy-5'-(methylthio)adenosine, and a triterpenoid.  

PubMed

Although Pleurotus abalonus is a well-known edible mushroom in Asia, there is a dearth of information on its antioxidant activity. The present report is the first one focused on the purification and characterization of 9-beta-d-ribofuranosidoadenine (ADO), 5'-deoxy-5'-(methylthio) adenosine (MTA) and a triterpenoid complex from P. abalonus. Different antioxidant activities including inhibitory effects on hemolysis and lipid peroxidation in brain and kidney homogenates as well as significant synergistic effect on scavenging of hydroxyl radicals were demonstrated, which lays a foundation for the development of P. abalonus as a natural antioxidant applied in medicine. PMID:23892470

Zhang, Ye Ni; Song, Min; Ng, Tzi Bun; Zhao, Li; Liu, Fang

2013-06-28

375

Prooxidant and antioxidant properties of human serum ultrafiltrates toward LDL: important role of uric acid.  

PubMed

Oxidized LDL is present within atherosclerotic lesions, demonstrating a failure of antioxidant protection. A normal human serum ultrafiltrate of Mr below 500 was prepared as a model for the low Mr components of interstitial fluid, and its effects on LDL oxidation were investigated. The ultrafiltrate (0.3%, v/v) was a potent antioxidant for native LDL, but was a strong prooxidant for mildly oxidized LDL when copper, but not a water-soluble azo initiator, was used to oxidize LDL. Adding a lipid hydroperoxide to native LDL induced the antioxidant to prooxidant switch of the ultrafiltrate. Uric acid was identified, using uricase and add-back experiments, as both the major antioxidant and prooxidant within the ultrafiltrate for LDL. The ultrafiltrate or uric acid rapidly reduced Cu2+ to Cu+. The reduction of Cu2+ to Cu+ may help to explain both the antioxidant and prooxidant effects observed. The decreased concentration of Cu2+ would inhibit tocopherol-mediated peroxidation in native LDL, and the generation of Cu+ would promote the rapid breakdown of lipid hydroperoxides in mildly oxidized LDL into lipid radicals. The net effect of the low Mr serum components would therefore depend on the preexisting levels of lipid hydroperoxides in LDL. These findings may help to explain why LDL oxidation occurs in atherosclerotic lesions in the presence of compounds that are usually considered to be antioxidants. PMID:12562831

Patterson, Rebecca A; Horsley, Elizabeth T M; Leake, David S

2002-12-16

376

Functional characterization and role of INrf2 in antioxidant response element-mediated expression and antioxidant induction of NAD(P)H:quinone oxidoreductase1 gene  

Microsoft Academic Search

Antioxidant response element (ARE) and nuclear transcription factor Nrf2 are known to regulate expression and coordinated induction of NQO1 and other detoxifying enzyme genes in response to antioxidants and xenobiotics. A cytosolic inhibitor of Nrf2, INrf2, that retains Nrf2 in the cytoplasm, was cloned and sequenced. Treatment of cells with antioxidants and xenobiotics results in the release of Nrf2 from

Saravanakumar Dhakshinamoorthy; Anil K Jaiswal

2001-01-01

377

Quality and antioxidant properties of a reduced-sugar pomegranate juice jelly with an aqueous extract of pomegranate peels.  

PubMed

In the present study, the production of a reduced-sugar pomegranate juice jelly supplemented with an aqueous extract of pomegranate peel (PE) is described. Influence of different carbohydrate polymers (guar (G), xanthan (X) and tragacanth (T) gums) on rheological properties was studied. Combination GXT presented the most similar rheological behaviour to commercial jelly. Jelly (J) and jelly with PE (JE) were stored at 4°C over an 8week period for physical, chemical, antioxidant, microbiological and sensory analysis. J and JE showed similar values for °Brix, colour and Aw, though the pH of JE was lower than J. Thiol and phenolic compounds were higher in JE than in J. Antioxidant activity (radical scavenging activity and autoxidation of linoleic acid) was higher in JE than in J at 0weeks, and were decreasing with time. Pomegranate juice with additives was generally less accepted than J and JE. PMID:23017400

Ventura, Janeth; Alarcón-Aguilar, Francisco; Roman-Ramos, Rubén; Campos-Sepulveda, Efraín; Reyes-Vega, Maria L; Daniel Boone-Villa, V; Jasso-Villagómez, Edgar Iván; Aguilar, Cristóbal N

2012-07-15

378

Effects of Athamanta turbith fruit essential oils on CCl4-induced hepatic failure in mice and their antioxidant properties.  

PubMed

The effects of essential oils isolated from mature fruits of Athamanta turbith ssp. hungarica (Borbás) Tutin and A. turbith ssp. haynaldii (Borbás & Uechtr.) Tutin (Umbelliferae) on some liver biochemical parameters in mice intoxicated with carbon tetrachloride were investigated. Pretreatment with both essential oils extenuated the effects caused by carbon tetrachloride. In order to investigate in vitro antioxidant properties of the oils, three methods were applied: scavenging of both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radicals, as well as a test of inhibition of Fe(2+)/ascorbic-induced lipid peroxidation. Investigated essential oils exhibited modest antioxidant capacity. Therefore, their influence on biochemical parameters in intoxicated animals might be linked to the inhibition of enzymes (cytochrome P450 2E1) involved in metabolic activation of halomethanes. PMID:19927273

Tomi?, Ana; Bozin, Biljana; Samojlik, Isidora; Milenkovi?, Marina; Mimica-Duki?, Neda; Petrovi?, Silvana

2010-05-01

379

In vivo and in vitro antioxidant activity and hepatoprotective properties of polyphenols from Halimeda opuntia (Linnaeus) Lamouroux.  

PubMed

Antioxidant activity and hepatoprotective properties of the aqueous extract and tetrahydrofuran-extracted phenolic fractions of Halimeda opuntia (Linnaeus) Lamouroux were investigated in rats with chemically induced liver injury. Total polyphenols were determined by using the Folin-Ciocalteau reagent. Liver damage was induced by CCl(4) and assessed by a histological technique. Reverse transcription/polymerase chain reaction (RT/PCR) analysis showed increased superoxide dismutase (SOD) and catalase (CAT) gene expression and activities in the group treated with free phenolic acid (FPA) fractions of H. opuntia, suggesting inducing effects on both enzymes. In addition, rats treated with FPA fractions displayed lower liver thiobarbituric acid reactive substance (TBARS) levels than those observed for rats in the CCl(4)-treated group. These data suggest that the phenolic fractions from H. opuntia may protect the liver against oxidative stress-inducing effects of chemicals by modulating its antioxidant enzymes and oxidative status. PMID:22564347

de Oliveira e Silva, Ana Mara; Vidal-Novoa, Alexis; Batista-González, Ana Elsa; Pinto, José Ricardo; Portari Mancini, Dalva Assunção; Reina-Urquijo, Wilman; Mancini-Filho, Jorge

2012-01-01

380

An electron paramagnetic resonance study of the antioxidant properties of the nitroxide free radical TEMPO.  

PubMed

Previous reports of superoxide scavenging and ferroxidase-like activity of nitroxide free radicals have greatly increased interest in the ability of these compounds to protect cells against oxidative cellular damage. In the present study we investigated the antioxidant properties of the six membered nitroxide 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) in various assays. TEMPO (5 mM) inhibited the hydroxyl radical mediated generation of ethylene from 2-keto-4-methylthiobutyric acid to 31.2 +/- 4.0% of control values. Furthermore, we noted that TEMPO had the ability to maintain iron in its ferric form, a finding with strong implications for the interpretation of the ferricytochrome c reduction assay. TEMPO may be reduced to an electron paramagnetic resonance silent hydroxylamine by a number of pathways. TEMPO absorption intensity decay (TAID) was monitored to investigate the effects of hydrogen peroxide on Cu,Zn-SOD. TEMPO was found to effectively scavenge or suppress formation of hydroxyl radicals inside Cu,Zn-SOD. The generation of hydroxyl radical was confirmed by employing the conventional spin trapping agent DMPO. Using radical scavengers unable to penetrate the Cu,Zn-SOD enzyme (e.g., mannitol, ethanol, albumin) or compounds with access to copper within the Cu,Zn-SOD enzyme (azide and cyanide), we could not detect hydroxyl radicals outside the enzyme. Finally, since the electron paramagnetic resonance absorption intensity is directly proportional to the concentration of TEMPO spins, loss of absorption intensity provided information concerning radical-mediated processes. Therefore, the decay kinetics of TEMPO may be used as a very sensitive alternative to conventional spin traps. PMID:8138184

Voest, E E; van Faassen, E; Marx, J J

1993-12-01

381

Antioxidant activity and characterization of phenolic compounds from bacaba (Oenocarpus bacaba Mart.) fruit by HPLC-DAD-MS(n).  

PubMed

The phytochemicals in fruits have been shown to be major bioactive compounds with regard to health benefits. Bacaba (Oenocarpus bacaba Mart.) is a native palm fruit from the Brazilian savannah and Amazon rainforest that plays an important role in the diet of rural communities and is also a source of income for poor people. This paper reports the characterization and analyses of phenolics from bacaba fruit extract. The total phenolic content of bacaba fruit amounted to 1759.27 ± 1.01 mg GAE/100 g, the flavonoid content was 1134.32 ± 0.03 mg CTE/100 g, and the anthocyanin content was 34.69 ± 0.00 mg cyn-3-glc/100 g. The antioxidant activity was evaluated through different assays [ORAC, FRAP, DPPH, TEAC, and cellular antioxidant assay (CAA) assays] and revealed a significant antioxidant capacity for bacaba in comparison to the data available in the literature. The assignment of the phenolic compounds using HPLC-DAD-MS(n) was based on the evaluation of their UV-vis absorption maxima (?(max)) and mass spectral analyses, and 14 compounds were tentatively identified. The results suggest that bacaba fruits are a promising source of phenolics. PMID:22788720

Abadio Finco, Fernanda D B; Kammerer, Dietmar R; Carle, Reinhold; Tseng, Wen-Hsin; Böser, Sabrina; Graeve, Lutz

2012-07-26

382

Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity.  

PubMed

Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium containing 1% shrimp and crab shell powder (1:1) (w/v), 0.1% K(2)HPO(4), and 0.05% MgSO(4)·7H(2)O. The molecular weight of chitinase was determined by SDS-PAGE to be 36 kDa. The optimum pH, temperature, pH stability, and thermal stability of chitinase were about 8, 37°C, 5-12, and 40-80°C, respectively. The antioxidant activity of A. faecalis AU02 culture supernatant was determined through scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) as 84%, and the antioxidant compound was characterized by TLC and its FT-IR spectrum. The present study proposed that marine wastes can be utilized to generate a high-value-added product and that pharmacological studies can extend its use to the field of medicine. PMID:22131949

Annamalai, Neelamegam; Veeramuthu Rajeswari, Mayavan; Vijayalakshmi, Shanmugam; Balasubramanian, Thangavel

2011-01-22

383

Antioxidant and gastric cytoprotective prostaglandins properties of Cassia sieberiana roots bark extract as an anti-ulcerogenic agent  

PubMed Central

Background Cassia sieberiana is a savannah tree with a wide phytotherapeutic application including the use of its roots in the management of various stomach disorders including gastric ulcer, stomach pains and indigestion. The aim of the study is to evaluate the antioxidant, gastric cytoprotective prostaglandins, secretory phospholipase A2, phytochemical and acute toxicity properties of Cassia sieberiana roots bark extract in a bid to justify its phytotherapeutic applications in gastric ulcer. Methods Antioxidant and radical scavenging activities of the roots bark extract of Cassia sieberiana were assayed. Serum secretory phospholipase A2 (sPLA2) concentration and activity and the formation of gastric mucosal prostaglandins E2 (PGE2) and I2 (PGI2) were also assessed. Comparisons between means were performed using analysis of variance (ANOVA) followed by Students Standard Newman-Keuls post hoc analysis to determine statistical significance. P?antioxidant power and can scavenge hydroxyl radicals. The extract also possesses DPPH scavenging activity, can chelate ferrous ion and a dose-dependent protective effect against lipid peroxidation and free radical generation. Prostaglandin studies showed that the roots bark extract dose dependently increased gastric mucosal PGE2 and PGI2 levels and also decreased serum sPLA2 activity. Phytochemical analyses suggest that the roots extract contains polyhydroxyl/phenolic substances. Acute toxicity test showed no sign of toxicity up to a dose level of 2000?mg/kg body weight p.o. Conclusions C. sieberiana roots extract possesses significant antioxidant and gastric cytoprotective prostaglandin properties as well as serum secretory phospholipase A2 inhibitory activity which could be due to its content of polyhydroxy and/or phenolic substances. This may justify its use as an anti-ulcerogenic agent in traditional medicine in West Africa.

2012-01-01

384

The Improvement of The Endogenous Antioxidant Property of Stone Fish (Actinopyga lecanora) Tissue Using Enzymatic Proteolysis  

PubMed Central

The stone fish (Actinopyga lecanora) ethanolic and methanolic tissue extracts were investigated for total phenolic contents (TPCs) as well as antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging activity and ferric reducing antioxidant power (FRAP) assays. Both extracts showed low amount of phenolics (20.33 to 17.03?mg of gallic acid equivalents/100?g dried sample) and moderate antioxidant activity (39% to 34%??DPPH• radical scavenging activity and 23.95 to 22.30?mmol/100?mL FeSO4 FRAP value). Enzymatic proteolysis was carried out in order to improve the antioxidant activity using six commercially available proteases under their optimum conditions. The results revealed that the highest increase in antioxidant activity up to 85% was obtained for papain-generated proteolysate, followed by alcalase (77%), trypsin (75%), pepsin (68%), bromelain (68%), and flavourzyme (50%) as measured by DPPH• radical scavenging activity, whilst for the FRAP value, the highest increase in the antioxidant activity up to 39.2?mmol/100?mL FeSO4 was obtained for alcalase-generated proteolysate, followed by papain (29.5?mmol/100?mL FeSO4), trypsin (23.2?mmol/100?mL FeSO4), flavourzyme (24.7?mmol/100?mL FeSO4), bromelain (22.9?mmol/100?mL FeSO4), and pepsin (20.8?mmol/100?mL FeSO4). It is obvious that proteolysis of stone fish tissue by proteolytic enzymes can considerably enhance its antioxidant activity.

Bordbar, Sara; Ebrahimpour, Afshin; Abdul Hamid, Azizah; Abdul Manap, Mohd Yazid; Anwar, Farooq; Saari, Nazamid

2013-01-01

385

The improvement of the endogenous antioxidant property of stone fish (Actinopyga lecanora) tissue using enzymatic proteolysis.  

PubMed

The stone fish (Actinopyga lecanora) ethanolic and methanolic tissue extracts were investigated for total phenolic contents (TPCs) as well as antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH(•)) radical scavenging activity and ferric reducing antioxidant power (FRAP) assays. Both extracts showed low amount of phenolics (20.33 to 17.03 mg of gallic acid equivalents/100 g dried sample) and moderate antioxidant activity (39% to 34% DPPH(•) radical scavenging activity and 23.95 to 22.30 mmol/100 mL FeSO4 FRAP value). Enzymatic proteolysis was carried out in order to improve the antioxidant activity using six commercially available proteases under their optimum conditions. The results revealed that the highest increase in antioxidant activity up to 85% was obtained for papain-generated proteolysate, followed by alcalase (77%), trypsin (75%), pepsin (68%), bromelain (68%), and flavourzyme (50%) as measured by DPPH(•) radical scavenging activity, whilst for the FRAP value, the highest increase in the antioxidant activity up to 39.2 mmol/100 mL FeSO4 was obtained for alcalase-generated proteolysate, followed by papain (29.5 mmol/100 mL FeSO4), trypsin (23.2 mmol/100 mL FeSO4), flavourzyme (24.7 mmol/100 mL FeSO4), bromelain (22.9 mmol/100 mL FeSO4), and pepsin (20.8 mmol/100 mL FeSO4). It is obvious that proteolysis of stone fish tissue by proteolytic enzymes can considerably enhance its antioxidant activity. PMID:23586061

Bordbar, Sara; Ebrahimpour, Afshin; Abdul Hamid, Azizah; Abdul Manap, Mohd Yazid; Anwar, Farooq; Saari, Nazamid

2013-02-03

386

Comparative characterization of sweetpotato antioxidant genes from expressed sequence tags of dehydration-treated fibrous roots under different abiotic stress conditions.  

PubMed

Drought stress is one of the most adverse conditions for plant growth and productivity. The plant antioxidant system is an important defense mechanism and includes antioxidant enzymes and low-molecular weight antioxidants. Understanding the biochemical and molecular responses to drought is essential for improving plant resistance to water-limited conditions. Previously, we isolated and characterized expressed sequence tags (ESTs) from a full-length enriched cDNA library prepared from fibrous roots of sweetpotato subjected to dehydration stress (Kim et al. in BMB Rep 42:271-276, [5]). In this study, we isolated and characterized 11 sweetpotato antioxidant genes from sweetpotato EST library under various abiotic stress conditions, which included six intracellular CuZn superoxide dismutases (CuZnSOD), ascorbate peroxidase, catalase, glutathione peroxidase (GPX), glutathione-S-transferase, thioredoxin (TRX), and five extracellular peroxidase genes. The expression of almost all the antioxidant genes induced under dehydration treatments occurred in leaves, with the exception of extracellular swPB6, whereas some antioxidant genes showed increased expression levels in the fibrous roots, such as intracellular GPX, TRX, extracellular swPA4, and swPB7 genes. During various abiotic stress treatments in leaves, such as exposure to NaCl, cold, and abscisic acid, several intracellular antioxidant genes were strongly expressed compared with the expression of extracellular antioxidant genes. These results indicated that some intracellular antioxidant genes, especially swAPX1 and CuZnSOD, might be specifically involved in important defense mechanisms against oxidative stress induced by various abiotic stresses including dehydration in sweetpotato plants. PMID:23187736

Kim, Yun-Hee; Jeong, Jae Cheol; Lee, Haeng-Soon; Kwak, Sang-Soo

2012-11-27

387

Antioxidant enzymatic defences in human follicular fluid: characterization and age-dependent changes  

Microsoft Academic Search

The aim of this work was to study the antioxidant enzymatic defences in human follicular fluid and investigate their possible changes during reproductive ageing. To this end, we tested the specific activities and protein expression of enzymes involved in reactive oxygen species (ROS) scavenging and in detoxification of ROS byproducts in follicular fluid from young (range 27-32 years, n =

M. C. Carbone; C. Tatone; S. Delle Monache; R. Marci; D. Caserta; R. Colonna; F. Amicarelli

2003-01-01

388

Antioxidant genes of the emerald ash borer ( Agrilus planipennis): Gene characterization and expression profiles  

Microsoft Academic Search

Phytophagous insects frequently encounter reactive oxygen species (ROS) from exogenous and endogenous sources. To overcome the effect of ROS, insects have evolved a suite of antioxidant defense genes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione peroxidase (GPX). The emerald ash borer (Agrilus planipennis Fairmaire), an exotic invasive insect pest from Asia has killed millions of

Swapna Priya Rajarapu; Praveen Mamidala; Daniel A. Herms; Pierluigi Bonello; Omprakash Mittapalli

2011-01-01

389

Characterization of nitrated phenolic compounds for their anti-oxidant, pro-oxidant, and nitration activities.  

PubMed

Coffee is one of the most widely consumed beverages worldwide. Evidence of the health benefits and the important contribution of coffee brew to the intake of anti-oxidants in the diet has increased coffee consumption. Chlorogenic acid (ChA) and caffeic acid (CaA) are the major phenolic compounds in coffee. However, phenolic compounds, which are generally effective anti-oxidants, can become pro-oxidants in the presence of Cu(2+) to induce DNA damage under certain conditions. On the other hand, sodium nitrite (NaNO(2)) is widely used as a food additive to preserve and tinge color on cured meat and fish. It is possible that phenolic compounds react with NaNO(2) under acidic conditions, such as gastric juice. In this study, we identified compounds produced by the reaction between ChA or CaA in coffee and NaNO(2) in artificial gastric juice. The identified phenolic compounds and nitrated phenolic compounds were assessed for their anti-oxidant, pro-oxidant, and nitration activities by performing an in vitro assay. The nitrated phenolic compounds seemed to show increased anti-oxidant activity and decreased pro-oxidant activity. However, one nitrated CaA compound that has a furoxan ring showed the ability to release NO(2)(-) in the neutral condition. PMID:21723849

Iwasaki, Yusuke; Nomoto, Maki; Oda, Momoko; Mochizuki, Keisuke; Nakano, Yuki; Ishii, Yuji; Ito, Rie; Saito, Koichi; Umemura, Takashi; Nishikawa, Akiyoshi; Nakazawa, Hiroyuki

2011-06-24

390

Characterization of phenolics content and antioxidant activity of different beer types.  

PubMed

Despite the wide literature describing the biological effects of polyphenols, scarce data are available on their content in the human diet. This study examined total polyphenols content, free and total phenolic acids profile, and antioxidant activity of different commercial beers types (abbey, ale, bock, wheat, lager, pilsner, and dealcoholized). Ferulic acid is by far the most abundant phenolic acid in beers, followed by sinapic, vanillic, caffeic, p-coumaric, and 4-hydroxyphenylacetic acids. Ferulic, caffeic, syringic, sinapic, and, to a lesser extent, vanillic acids are present in beers mainly as bound forms, whereas p-coumaric and 4-hydroxyphenylacetic acids are generally present equally in free and bound forms. Total polyphenols and phenolic acids contents greatly vary among different beer types (i.e., total polyphenols, from 366 ?g/mL gallic acid equivalents for dealcoholized beers to 875 ?g/mL gallic acid equivalents for bock beers, with higher values measured in bock, abbey, and ale beers and lower values in dealcoholized beers). Similarly, the antioxidant activity measured with the ferric reducing antioxidant power (FRAP) assay is remarkably different depending on beer type (from 1525 ?M for dealcoholized beers to 4663 ?M for bock beers), with higher values in bock, abbey, and ale beers and lower values in dealcoholized beers. FRAP values strictly correlate with polyphenols and phenolic acids content. The contribution of single phenolic acids to the antioxidant activity measured with FRAP assay was also studied. PMID:20822144

Piazzon, Alessandro; Forte, Monica; Nardini, Mirella

2010-10-13

391

Characterization of flavonoids from Dryopteris erythrosora and evaluation of their antioxidant, anticancer and acetylcholinesterase inhibition activities.  

PubMed

The profiles and bioactivities of flavonoids extracted from Dryopteris erythrosora were investigated. The total flavonoid content in full plant of D. Erythrosora is about 14.33%. The main flavonoids in D. Erythrosora were identified as gliricidin 7-O-hexoside, apigenin7-O-glucoside, quercetin 7-O-rutinoside, quercetin 7-O-galactoside, keampferol 7-O-gentiobioside, keampferol-3-O-rutinoside, myricetin 3-O-rhamnoside and quercitrin by means of HPLC-DAD-ESI-MS. Flavonoids (0.36mg/ml) extract from D. erythrosora showed similar 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH()), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS()), superoxide anion scavenging potential and ferric reducing antioxidant potential (FRAP) with that of rutin (0.80mg/ml). However, the antioxidant power by FRAP assay of 0.36mg/ml flavonoids extract from D. erythrosora was much weaker than that of 0.80mg/ml rutin. Moreover, the flavonoids extract from D. erythrosora showed obvious cytotoxic effects on A549 cells. The antioxidant activities of flavonoids extracts from 69 ferns showed a significant reciprocal proportion to the total flavonoids contents. The flavonoids extract from D. erythrosora exhibited a dose-dependent inhibition against acetylcholinesterase. Moreover, the anticancer activity slightly increased with improving antioxidant potential of fern flavonoids. Fern flavonoids are excellent function foods. PMID:23063594

Cao, Jianguo; Xia, Xian; Chen, Xuefei; Xiao, Jianbo; Wang, Quanxi

2012-10-12

392

Polyphenolic Contents and Antioxidant Properties of Different Grape (V. vinifera, V. labrusca, and V. hybrid) Cultivars  

PubMed Central

The polyphenolic contents and the antioxidant activity of the skins and pulps of different grape cultivars were estimated using HPLC and DPPH antioxidant assay, respectively. The phenolics and flavonoids identified were quercetin, kaempferol, caffeic acid, p-coumaric acid, cinnamic acid, and (?)-epicatechin. The total phenolic contents were found to be the highest in the grape skin of Flouxa (>400?mg/100?g), followed by Campbell Early and Tamnara (>300?mg/100?g), and then by Red Globe and Ruby Seedless (>250?mg/100?g), and the total phenolic content was the lowest in Italia and Delaware (<60?mg/100 g). The antioxidant activities of the grape extracts varied from 12.5% (Ruby Seedless) to 60.2% (Hongiseul) for skins, whereas the antioxidant activities of the grape extracts varied from 35.4% (Campbell Early) to 84.5% (Hongiseul) for pulps. The grape pulps have stronger antioxidant activities than those of the grape skins. Our results suggest that the phenolic and flavonoid contents in extracts of grape skins and pulps showed statistically significant correlations with the free radical scavenging activity.

Nile, Shivraj Hariram; Kim, S. H.; Ko, Eun Young; Park, Se Won

2013-01-01

393

Antioxidant properties of ?-seleno amines against lipid peroxidation in rat brain and liver.  

PubMed

?-Seleno amines were screened for in vitro antioxidant activity. The compounds (C1-C4) were tested against lipid peroxidation induced by iron and sodium nitroprusside in rat brain and liver homogenates. The compounds showed inhibition against thiobarbituric acid reactive species (TBARS) induced by different pro-oxidants (10?M FeSO(4) and 5?M sodium nitroprusside (SNP) in rat brain and liver homogenates. The compounds exhibited strong antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and phosphomolybdenum assays. The IC(50) values revealed that the ?-seleno amines in which the amino group was protected with protecting groups tert-butyloxycarbonyl (Boc) and Tosyl (Ts) groups showed better antioxidant profiles compared to the free monoselenides. The total antioxidant activity of C1, C2, C3 and C4 were found to be 85.2±11.5, 114±7.9, 138±8.5, 125.81±5.2?M/ml of ascorbic acid respectively. Therefore, these compounds may be used as synthetic antioxidants. PMID:22797322

Sabir, S M; Salman, Syed M; Rocha, J B T

2012-06-26

394

Evaluation of Antioxidant Properties and Mineral Composition of Purslane (Portulaca oleracea L.) at Different Growth Stages  

PubMed Central

The main objective of this research was to appraise the changes in mineral content and antioxidant attributes of Portulaca oleracea over different growth stages. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) assays. The iodine titration method was used to determine the ascorbic acid content (AAC). DPPH scavenging (IC50) capacity ranged from 1.30 ± 0.04 to 1.71 ± 0.04 mg/mL, while the ascorbic acid equivalent antioxidant activity (AEAC) values were 229.5 ± 7.9 to 319.3 ± 8.7 mg AA/100 g, total phenol content (TPC) varied from 174.5 ± 8.5 to 348.5 ± 7.9 mg GAE/100 g. AAC 60.5 ± 2.1 to 86.5 ± 3.9 mg/100 g and FRAP 1.8 ± 0.1 to 4.3 ± 0.1 mg GAE/g. There was good correlation between the results of TPC and AEAC, and between IC50 and FRAP assays (r2 > 0.9). The concentrations of Ca, Mg, K, Fe and Zn increased with plant maturity. Calcium (Ca) was negatively correlated with sodium (Na) and chloride (Cl), but positively correlated with magnesium (Mg), potassium (K), iron (Fe) and zinc (Zn). Portulaca olerecea cultivars could be used as a source of minerals and antioxidants, especially for functional food and nutraceutical applications.

Uddin, Md. Kamal; Juraimi, Abdul Shukor; Ali, Md. Eaqub; Ismail, Mohd Razi

2012-01-01

395

Synthesis and antioxidant properties of substituted 2-phenyl-1H-indoles.  

PubMed

In this study, we report the design, synthesis and antioxidant activity of a series of substituted 2-(4-aminophenyl)-1H-indoles and 2-(methoxyphenyl)-1H-indoles. The new compounds are structurally related to the known indole-based antioxidant lead compound melatonin (MLT), and the antitumour 2-(4-aminophenyl)benzothiazole and 2-(3,4-dimethoxyphenyl)benzothiazole series. Efficient access to the target 2-phenylindoles was achieved via Fischer indole synthesis between substituted phenylhydrazines and acetophenones. 2-(4-Aminophenyl)indoles (such as the 6-fluoro analogue 3b) in particular showed potent antioxidant activity in the DPPH and superoxide radical scavenging assays (80% and 81% inhibition at 1mM concentration of 3b, respectively), at a level comparable with the reference standard MLT (98% and 75% at 1 mM). PMID:23540647

Karaaslan, Cigdem; Kadri, Hachemi; Coban, Tulay; Suzen, Sibel; Westwell, Andrew D

2013-03-01

396

Antioxidant and Anti-Inflammatory Properties of Longan (Dimocarpus longan Lour.) Pericarp  

PubMed Central

This study examined the antioxidant and anti-inflammatory activities of the water extract of longan pericarp (WLP). The results showed that WLP exhibited radical scavenging, reducing activity and liposome protection activity. In addition, WLP also inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophages. Further, administration of WLP, in the range of 100–400?mg/kg, showed a concentration-dependent inhibition on paw edema development following carrageenan (Carr) treatment in mice. The anti-inflammatory effects of WLP may be related to NO and tumor necrosis factor (TNF-?) suppression and associated with the increase in the activities of antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase. Overall, the results showed that WLP might serve as a natural antioxidant and inflammatory inhibitor.

Huang, Guan-Jhong; Wang, Bor-Sen; Lin, Wei-Chao; Huang, Shyh-Shyun; Lee, Chao-Ying; Yen, Ming-Tsung; Huang, Ming-Hsing

2012-01-01

397

Influence of lipid composition on physicochemical properties of nanoliposomes encapsulating natural dipeptide antioxidant l-carnosine.  

PubMed

Natural dipeptide antioxidants (l-carnosine) are recieving increasing attention because of their noticeable potential as biopreservatives in food recent technology. Encapsulation of antioxidants by nanoliposomes could represent an ameliorative approach to overcome the problems related to the direct application of these antioxidant peptides in food. In this study, nanoliposomes prepared from different lipids (DOPC, POPC and DPPC) by thin film hydration method, were assessed by considering their size, ?-potential, phase transition temperature and fluidity. One important parameter of interest in this article was to compare the encapsulation efficacy of l-carnosine in three different nanoliposomes using a rapid and precise approach (1)H NMR without the need for physical separation of entrapped and non-entrapped l-carnosine. Furthermore, the morphology of small unilamellar nanoliposomes with different compositions on mica surface was investigated using Atomic Force Microscopy. PMID:23107672

Maherani, Behnoush; Arab-Tehrany, Elmira; Kheirolomoom, Azadeh; Cleymand, Franck; Linder, Michel

2012-03-01

398

Antioxidant properties of benzylchroman derivatives from Caesalpinia sappan L. against oxidative stress evaluated in vitro.  

PubMed

The antioxidant activity of extracts from Caesalpinia sappan L. (CSL) was studied in vitro by evaluating the total phenolics, measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) and on reactive nitrogen species (RNS), and measuring the inhibitory effect on Cu(2+)-induced human low-density lipoprotein (LDL) oxidation. The CSL extracts were found to have a potent scavenging activity against all of the reactive species tested, as well as an inhibitory effect on LDL oxidation, especially in the ethyl acetate (EA) fraction. Therefore, we isolated and identified benzylchroman derivatives sappanchalcone (1) and 3'-deoxy-4-O-methylepisappanol (2) from the EA fraction of CSL and their antioxidant activities were evaluated. The studied CSL extracts and the compounds 1 and 2 were revealed to be very effective against the evaluated pro-oxidant species, including ROS and RNS. PMID:20105050

Lee, Min-Ja; Lee, Hye-Sook; Kim, Hyuck; Yi, Hyo-Seung; Park, Sun-Dong; Moon, Hyung-In; Park, Won-Hwan

2010-10-01

399

Antioxidant properties of an endogenous thiol: Alpha-lipoic acid, useful in the prevention of cardiovascular diseases.  

PubMed

In the past few years, a growing interest has been given to the possible antioxidant functions of a natural acid, synthesized in human tissues: alpha-lipoic acid (ALA). Both the oxidized (disulfide) and reduced (dithiol: dihydrolipoic acid, DHLA) forms of ALA show antioxidant properties. ALA administered in the diet accumulates in tissues, and a substantial part is converted to DHLA via a lipoamide dehydrogenase. Commercial ALA is usually a racemic mixture of the R and S forms. Chemical studies have indicated that ALA scavenges hydroxyl radicals, hypochlorous acid, and singlet oxygen. ALA exerts antioxidant effects in biological systems not only through direct ROS quenching but also via transition metal chelation. ALA has been shown to possess a number of beneficial effects both in the prevention and treatment of diabetes in experimental conditions. ALA presents beneficial effects in the management of symptomatic diabetic neuropathy and has been used in this context in Germany for more than 30 years. In cardiovascular disease, dietary supplementation with ALA has been successfully employed in a variety of in vivo models: ischemia-reperfusion, heart failure, and hypertension. More mechanistic and human in vivo studies are needed to determine whether optimizing the dietary intake of ALA can help to decrease cardiovascular diseases. A more complete understanding of cellular biochemical events that influence oxidative damage is required to guide future therapeutic advances. PMID:19998523

Ghibu, Stéliana; Richard, Carole; Vergely, Catherine; Zeller, Marianne; Cottin, Yves; Rochette, Luc

2009-11-01

400

Use of liposomes as membrane models to evaluate the contribution of drug-membrane interactions to antioxidant properties of etodolac.  

PubMed

This work stresses the need to combine antioxidant assays and drug-membrane interaction studies to describe more accurately the antioxidant profile of non-steroidal anti-inflammatory drugs (NSAIDs). Different experiments performed in liposomes and aqueous solution were compared and used to evaluate the protective effect of etodolac in lipid peroxidation. Lipid peroxidation was induced by the peroxyl radical (ROO*) derived from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and hydroxyl radical (HO*) generated by the Fenton reaction and was assessed by the fluorescence intensity decay of three fluorescence probes with distinct lipophilic properties--fluorescein; hexadecanoyl aminofluorescein (HDAF) and diphenylhexatriene propionic acid (DPHPA). Membrane fluidity changes due to lipid peroxidation were also evaluated by steady-state anisotropy measurements. Interactions of etodolac with lipid bilayers were evaluated by membrane zeta-potential measurements. Results indicate a drug location near the membrane surface and show that etodolac can scavenge the radicals studied but to a variable extent, depending on the assayed media and reactive species. The use of different probes and liposomes as membrane mimetic systems allowed us to conclude that membrane lipoperoxidation is not only related to the scavenging characteristics of the antioxidants, but also to their ability to interact with lipid bilayers. PMID:18796242

Lúcio, Marlene; Ferreira, Helena; Lima, José L F C; Reis, Salette

2008-01-01

401

[Studies of the blood antioxidant system and oxygen-transporting properties of human erythrocytes during 105-day isolation].  

PubMed

Effects of strict 105-d isolation on blood antioxidant status, erythrocyte membrane processes and oxygen-binding properties of hemoglobin were studied in 6 male volunteers (25 to 40 y.o.) in ground-based simulation of a mission to Mars (experiment Mars-105). The parameters were measured using venous blood samples collected during BDC, on days 35, 70 and 105 of the experiment and on days 7 and 14-15 after its completion. Methods of biochemistry (determination of enzyme activity and thin-layer chromatography) and biophysical (laser interference microscopy, Raman spectroscopy) showed changes in relative content of lipid and phospholipid fractions suggesting growth of membrane microviscosity and increase in TBA-AP (active products of lipids peroxidation interacting with thiobarbituric acid). A significant increase in glucose-6-phosphate dehydrogenase and superoxide dismutase activities against reduction of catalase activity points to both reparative processes in erythrocytes and disbalance between the number of evolving active forms of oxygen and antioxidant protection mechanisms in cells. Hemoglobin sensitivity of oxygen and blood level of oxyhemoglobin were found to increase, too. It is presumed that adaptation of organism to stresses experienced during and after the experiment may destroy balance of the antioxidant protection systems which is conducive to oxidation of membrane phospholipids, alteration of their content, increase of membrane microviscosity and eventual failure of the gas-exchange function of erythrocytes. PMID:21675192

Brazhe, N A; Ba?zhumanov, A A; Parshina, E Iu; Iusipovich, A I; Akhalaia, M Ia; Iarlykova, Iu V; Labetskaia, O I; Ivanova, S M; Morukov, B V; Maksimov, G V

402

The antioxidant properties of new dimer and two monomers of phenolic acid amides isolated from Limoniastrum guyonianum.  

PubMed

Limoniastramide, a new dimer of phenolic acid amide, isolated from Limoniastrum guyonianum, along with two natural monomers N-E-caffeoyl tyramine (1) and N-E-feruloyl tyramine (2), using centrifugal partition chromatography (CPC). Their structures were determined on the basis of spectroscopic data analysis. We investigate the antioxidant activities of Limoniastrum amides using various in vitro assays. Results showed that N-E-feruloyl tyramine and N-E-caffeoyl tyramine exhibited the highest DPPH scavenging activity compared to the dimer (IC50=0.5, 0.6 and 6.5?g/ml, respectively). In addition, they have significant capacities to inhibit the bleaching of ?-carotene. Limoniastramide presented the best activity with an IC50 value equal to 8?g/ml. Finally, the N-E-caffeoyl tyramine showed the highest reducing power (EC50=26?g/ml) compared to the other molecules. The present study found that L. guyonianum amides have effective in vitro antioxidant and radical scavenging activity which can be used in pharmacological and food industry due to their antioxidant properties. PMID:24176369

Trabelsi, Najla; Oueslati, Samia; Ksouri, Riadh; Nassra, Merian; Marchal, Axel; Krisa, Stéphanie; Abdelly, Chedly; Mérillon, Jean-Michel; Waffo-Téguo, Pierre

2013-09-25

403

Antioxidant properties of Taraxacum officinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice.  

PubMed

Acetaminophen (APAP) hepatotoxicity has been related to several cases of hepatitis, cirrhosis, and hepatic transplant. As APAP hepatotoxicity is related to reactive oxygen species (ROS) formation and excessive oxidative stress, natural antioxidant compounds have been tested as an alternative therapy to diminish the hepatic dysfunction induced by APAP. Taraxacum officinale Weber (Family Asteraceae), commonly known as dandelion, is used for medicinal purposes because of its choleretic, diuretic, antioxidant, anti-inflammatory, and hepatoprotective properties. This study evaluated the hepatoprotective activity of T. officinale leaf extract against APAP-induced hepatotoxicity. T. officinale was able to decrease thiobarbituric acid-reactive substance levels induced by 200?mg/kg APAP (p.o.), as well as prevent the decrease in sulfhydryl levels caused by APAP treatment. Furthermore, histopathological alterations, as well as the increased levels of serum aspartate and alanine aminotransferases caused by APAP, were prevented by T. officinale (0.1 and 0.5?mg/mL). In addition, T. officinale extract also demonstrated antioxidant activity in vitro, as well as scavenger activity against 2,2-diphenyl-1-picrylhydrazyl and nitric oxide radicals. Our results clearly demonstrate the hepatoprotective effect of T. officinale against the toxicity induced by APAP. The possible mechanisms involved include its scavenger activities against ROS and reactive nitrogen species, which are attributed to the content of phenolic compounds in the extract. PMID:22424457

Colle, Dirleise; Arantes, Leticia Priscilla; Gubert, Priscila; da Luz, Sônia Cristina Almeida; Athayde, Margareth Linde; Teixeira Rocha, João Batista; Soares, Félix Alexandre Antunes

2012-03-16

404

Contribution of phenolic compounds to the antioxidant potential and type II diabetes related enzyme inhibition properties of Pongamia pinnata L. Pierre seeds  

Microsoft Academic Search

The methanolic extract of Pongamia pinnata L. Pierre (locally called as karanja) seed materials, an underutilized food legume collected from India was analyzed for antioxidant and type II diabetes related enzyme inhibition properties. The methanolic extract of raw seeds contained total free phenolic content of 14.85±0.32g catechin equivalent\\/100g extract DM. Encouraging levels of ferric reducing\\/antioxidant power (FRAP, 1179mmol Fe[II]\\/mg extract),

Vellingiri Vadivel; Hans K. Biesalski

2011-01-01

405

Structure activity relationship of antioxidative property of flavonoids and inhibitory effect on matrix metalloproteinase activity in UVA-irradiated human dermal fibroblast  

Microsoft Academic Search

Collagenase, a matrix metalloproteinases (MMPs), is a key regulator in the photoaging process of skin due to the reactive\\u000a oxygen species generated after exposure to ultraviolet A (UVA). Flavonoid compounds have been demonstrated to possess antioxidant\\u000a properties, and could be useful in the prevention of photoaging. In this study, to investigate the structure-activity relationship\\u000a of flavonoid compounds on their antioxidant

Gwan-Sub Sim; Bum-Chun Lee; Ho Seung Cho; Jae Woong Lee; Jin-Hwa Kim; Dong-Hwan Lee; Jin-Hui Kim; Hyeong-Bae Pyo; Dong Cheul Moon; Yeo Pyo Yun; Jin Tae Hong

2007-01-01

406

Characterization of mechanical properties of Inconel alloys.  

National Technical Information Service (NTIS)

The microstructure and mechanical properties of Inconel 60 and Inconel 690 steam generator tube test-produced by Sammi Steel Co. Ltd. were analyzed to develop the Inconel steam generator tube for nuclear power plant. 2. The mechanical properties such as y...

S. H. Hong H. Y. Kim W. H. Sohn Y. C. Ahn

1997-01-01

407

Comparisons on the Functional Properties and Antioxidant Activity of Spray-Dried and Freeze-Dried Egg White Protein Hydrolysate  

Microsoft Academic Search

Freeze-dried and spray-dried egg white protein hydrolysates (FD-EWPH, SD-EWPH) prepared by papain were evaluated for their\\u000a proximate composition, color, molecular weight distribution, physical structures, functional properties, and antioxidant activity.\\u000a FD-EWPH and SD-EWPH had similar proximate composition and molecular weight distribution, but they had different color and\\u000a physical structures. There was no significant difference (P?>?0.05) in solubility among egg white protein

Chen Chen; Yu-Jie Chi; Wei Xu

408

Isolation and characterization of antioxidant and antibacterial compound from mango ginger (Curcuma amada Roxb.) rhizome.  

PubMed

The chloroform extract of mango ginger (Curcuma amada Roxb.) rhizome was subjected to antioxidant activity-guided purification by repeated silica gel column chromatography to obtain a pure antioxidant compound. The structure was deduced by analyzing UV, IR, liquid chromatography-mass spectrometry (LC-MS) and two-dimensional heteronuclear multiple quantum coherence transfer spectroscopy (2D-HMQCT) NMR spectral data, and named it as "Amadannulen", a novel compound. It exhibited DPPH radical scavenging activity, super oxide radical scavenging activity, lipid peroxidation inhibitory activity and metal chelating activity. Amadannulen also showed antibacterial activity against both Gram-positive and Gram-negative bacteria tested. It also exhibited bactericidal activity against M. luteus, B. cereus and B. subtilis. PMID:17223394

Policegoudra, R S; Abiraj, K; Channe Gowda, D; Aradhya, S M

2007-01-16

409

Preparation of starch-sodium lignosulfonate graft copolymers via laccase catalysis and characterization of antioxidant activity.  

PubMed

Graft copolymers of waxy maize starch and sodium lignosulfonate (SLS) were prepared by Trametes versicolor laccase catalysis in aqueous solution. Amount of SLS grafted based on phenol analysis was 0.5% and 1.0% in the absence and presence of 1-hydroxybenzotriazole (HBT), respectively. Starch-SLS graft copolymers were effective antioxidants as judged by 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The presence of laccase caused a reduction in starch molecular weight although a cross-linked gel fraction was also observed when HBT was present. This new method for preparing starch chemically modified with phenolic compounds is simple and the resulting antioxidant polymers have potential in food, cosmetic and packaging applications. PMID:23121948

Shogren, Randal L; Biswas, Atanu

2012-08-31

410

Evaluation of the antioxidant and antibacterial properties of various solvents extracts of Annona squamosa L. leaves  

Microsoft Academic Search

The present work was conducted aiming to evaluate the effect of different solvent extracts on the antioxidant and antibacterial activities of Annona squamosa L. leaves. Four solvents were chosen for the study namely; methanol 80%, acetone 50%, ethanol 50% and boiling water. Acetone and boiling water gave the highest extraction yields as compared to methanol and ethanol. Total phenolic contents

Ghadir A. El-Chaghaby; Abeer F. Ahmad; Eman S. Ramis

411

Antioxidant, anti-inflammatory, and antimicrobial properties of garlic and onions  

Microsoft Academic Search

Purpose – The purpose of this paper is to provide a comprehensive overview of multiple functions and their underlying mechanisms for two common spices, garlic and onion, containing organosulphur compounds. Design\\/methodology\\/approach – Literature review of chemistry, physiology, molecular biology, clinical studies. Findings – Both garlic and onions exert their effects on human health via multiple different functions, including antioxidant, anti-inflammatory,

Emily A. Wilson; Barbara Demmig-Adams

2007-01-01

412

The in vitro and in vivo antioxidant properties of seabuckthorn ( Hippophae rhamnoides L.) seed oil  

Microsoft Academic Search

The antioxidant capacity of seabuckthorn (Hippophae rhamnoides L.) seed oil was investigated with a number of established in vitro assays and in an in vivo study of carbon tetrachloride (CCl4)-induced oxidative stress in mice. The results showed that DPPH radical scavenging activity, ferrous ion chelating activity, reducing power and inhibition of lipid peroxidation activity all increased with increasing concentrations of

Hung-Chih Ting; Yu-Wen Hsu; Chia-Fang Tsai; Fung-Jou Lu; Ming-Chih Chou; Wen-Kang Chen

2011-01-01

413

Antioxidant defense properties of Arctic amphipods: comparison between deep-, sublittoral and surface-water species  

Microsoft Academic Search

The toxic reactive oxygen species (ROS), produced naturally in an oxygenated environment, led to the development of antioxidant defenses by aerobic organisms to prevent tissue damage. Polar marine waters are regarded as a strongly prooxidant ecosystem, due to the high dissolved oxygen level and intense UV radiation during summer, while the deep waters are considered refuges against oxidative stress. In

L. Camus; B. Gulliksen

2005-01-01

414

Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions.  

PubMed

Antioxidant and antimicrobial activities as well as the quantity of phenolic substances (total phenol, flavonoid and phenolic acid contents) were determined in aqueous extracts of leaves, stems and flowers of Moltkia petraea (Tratt.) Griseb. from two mountainous localities (Sveti Jure and Snijeznica) in Croatia. In addition, the profile of phenolic acids was analyzed by UPLC-MS/MS. Antioxidant activities of all extracts in different test systems, namely the DPPH radical scavenging, reducing power assay and chelating activity, increased with extract concentration. Activity of the extracts from Snijeznica in beta-carotene-linoleic acid assay did not differ from the activity of standard, BHA. The leaf extracts from Snijeznica demonstrated superior antioxidant activity in most of the assays, while the stem extract from the same locality was the most effective Fe(2+) ion chelator. In general, the extracts from Snijeznica were more effective antioxidants than the corresponding extracts from Sveti Jure. The aqueous extracts of M. petraea did not show antimicrobial activity against bacteria and fungi tested in the diffusion and dilution assays. PMID:20304028

Konci?, M Zovko; Kremer, D; Gruz, J; Strnad, M; Bisevac, G; Kosalec, I; Samec, D; Piljac-Zegarac, J; Karlovi?, K

2010-03-19

415

Attachment of rhamnosyl glucoside on quercetin confers potent iron-chelating ability on its antioxidant properties  

Microsoft Academic Search

The pharmacological essence of the natural addition of rhamnosyl glucoside on quercetin that is commonly found in nature in medicinal plants is rather obscure. The present study therefore sought to compare the antioxidant activities of both compounds by comparing their ability to decolourise DPPH radicals, reduce Fe3+, chelate Fe2+, prevent deoxyribose degradation and inhibit hepatic thiobarbituric acid reactive substances induced

P. A. Omololu; J. B. T. Rocha; I. J. Kade

2011-01-01

416

MECHANISMS RESPONSIBLE FOR THE ANTIOXIDANT PROPERTIES OF CHLOROPHYLLIN: A PULSE RADIOLYSIS STUDY  

Microsoft Academic Search

A b s t r a c t Chlorophyllin, the water-soluble analogue of the ubiquitous green pigment chlorophyll has been shown to be a potent antioxidant and radioprotector under both in vitro and in vivo conditions. It is a potent protector of membrane damage and prevents protein oxidation, lipid peroxidation besides depletion of thiols induced by radiation, photosensitization and other

K. K. Boloor; T. P. A. Devasagayam

417

Antioxidant properties of Asparagus racemosus against damage induced by ?-radiation in rat liver mitochondria  

Microsoft Academic Search

The possible antioxidant effects of crude extract and a purified aqueous fraction of Asparagus racemosus against membrane damage induced by the free radicals generated during ?-radiation were examined in rat liver mitochondria. ?-Radiation, in the dose range of 75–900 Gy, induced lipid peroxidation as assessed by the formation of thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH). Using an

Jayashree P Kamat; Krutin K Boloor; Thomas P. A Devasagayam; S. R Venkatachalam

2000-01-01

418

Quality Characteristics and Antioxidant Properties of Breads Supplemented with Sugar Beet Molasses-Based Ingredients  

Microsoft Academic Search

Osmotic dehydration in sugar beet molasses as hypertonic medium was used to treat apples, plums, carrots and cabbage. Following the treatment, the fruits\\/vegetables were dried and ground. The obtained powders or pure beet molasses were incorporated into white wheat bread at 5 and 10% levels (flour basis). The results showed that the mineral content (K, Mg, Ca) and antioxidant potential

Bojana Filip?ev; Ljubinko Levi?; Marija Bodroža-Solarov; Nevena Mišljenovi?; Gordana Koprivica

2010-01-01

419

ANTIDIABETIC ACTIVITY OF AEGLE MARMELOS AND ITS RELATIONSHIP WITH ITS ANTIOXIDANT PROPERTIES  

Microsoft Academic Search

Abstract : Abstract : Abstract : Abstract : Oxidative stress induced by alloxan has been shown to damage pancreatic ?-cell and produce hyperglycemia in rats. Aegle marmelos leaf extract is being used in Ayurveda as a medicine for diabetes. The present study examined the action of Aegle marmelos against experimental diabetes as well as the antioxidant potential of the drug.

M. C. SABU; RAMADASAN KUTTAN

2004-01-01

420

Characterizing the elastic properties of tissues.  

PubMed

The quality of life of ageing populations is increasingly determined by age-related changes to the mechanical properties of numerous biological tissues. Degradation and mechanical failure of these tissues has a profound effect on human morbidity and mortality. Soft tissues have complex and intricate structures and, similar to engineering materials, their mechanical properties are controlled by their microstructure. Thus age-relate changes in mechanical behavior are determined by changes in the properties and relative quantities of microstructural tissue components. This review focuses on the cardiovascular system; it discusses the techniques used both in vivo and ex vivo to determine the age-related changes in the mechanical properties of arteries. PMID:22723736

Akhtar, Riaz; Sherratt, Michael J; Cruickshank, J Kennedy; Derby, Brian

2011-03-01

421

Supercritical fluid extraction of antioxidant and antimicrobial compounds from Laurus nobilis L. Chemical and functional characterization  

Microsoft Academic Search

Extraction of laurel leaves by using supercritical carbon dioxide was carried out on a supercritical fluid (SF) pilot-scale\\u000a plant. The extraction pressure and temperature were set to 250 bar and 60°C, respectively, using a 4% of ethanol as modifier.\\u000a The employed apparatus, owing to a two-stage separation, allowed us to obtain two different fractions (F1 and F2), whose antioxidant\\u000a and antimicrobial

S. Santoyo; R. Lloría; L. Jaime; E. Ibañez; F. J. Señoráns; G. Reglero

2006-01-01

422

Structural characterization and antioxidant activity of a low-molecular polysaccharide from Dendrobium huoshanense.  

PubMed

The present study aimed at investigating the structural features and antioxidant activities of a polysaccharide fraction (DHP1A) obtained from Dendrobium huoshanense, a precious herb medicine in China. DHP1A mainly consisted of mannose (Man), glucose (Glc) and a trace of galactose (Gal), with a molecular weight of 6700Da. Its backbone contained (1?4)-linked ?-D-Glcp, (1?6)-linked ?-D-Glcp and (1?4)-linked ?-D-Manp, with a branch of terminal ?-D-Galp. The in vitro antioxidant evaluation revealed that DHP1A had a remarkable inhibition effect on the FeCl2-induced lipid peroxidation. Furthermore, DHP1A pretreatment decreased the production of malondialdehyde (MDA), and restored the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), as well as the level of glutathione (GSH) in the livers of CCl4-treated mice. These results suggested that DHP1A was a potential antioxidant component in D. huoshanense. PMID:24096144

Tian, Chang-Cheng; Zha, Xue-Qiang; Pan, Li-Hua; Luo, Jian-Ping

2013-10-03

423

Oxidative stress as a risk factor for osteoporosis in elderly Mexicans as characterized by antioxidant enzymes  

PubMed Central

Background Oxidative stress (OxS) has recently been linked with osteoporosis; however, we do not know the influence of OxS as an independent risk factor for this disease. Methods We conducted a case-control study in 94 subjects ?60 years of age, 50 healthy and 44 with osteoporosis. We measured total antioxidant status, plasma lipid peroxides, antioxidant activity of superoxide dismutase and glutathione peroxidase (GPx), and calculated the SOD/GPx ratio. Bone mineral density was obtained at the peripheral DXA in calcaneus using a portable Norland Apollo Densitometer®. Osteoporosis was considered when subjects had a BMD of 2.5 standard deviations or more below the mean value for young adults. Results GPx antioxidant activity was significantly lower in the group of subjects with osteoporosis in comparison with the group of healthy subjects (p < 0.01); in addition, the SOD/GPx ratio was significantly higher in the group of individuals with osteoporosis (p < 0.05). In logistic regression analysis, we found OxS to be an independent risk factor for osteoporosis (odds ratio [OR] = 2.79; 95% confidence interval [95% CI] = 1.08–7.23; p = 0.034). Conclusion Our findings suggest that OxS is an independent risk factor for osteoporosis linked to increase of SOD/GPx ratio.

Sanchez-Rodriguez, Martha A; Ruiz-Ramos, Mirna; Correa-Munoz, Elsa; Mendoza-Nunez, Victor Manuel

2007-01-01

424

Purification and characterization of a novel ~18 kDa antioxidant protein from Ginkgo biloba seeds.  

PubMed

Ginkgo biloba seeds are widely used as a food and traditional medicine in China. In the present study, a novel antioxidant protein named GBSP was purified from Ginkgo biloba seeds. The protein (GBSP) was purified by homogenization of Ginkgo biloba seed powder in saline solution, 70% ammonium sulphate precipitation, filtration on a DEAE-Cellulose52 anion exchange column, gel filtration on a Sephadex G-50 column, and preparative chromatography on a C(18) column using RP-HPLC. GBSP showed an apparent molecular weight of 18 kDa by SDS-PAGE and MALDI-TOF/MS analyses. The amino acid sequence obtained by MALDI-TOF/TOF MS analysis showed GBSP was a novel protein, as no matching protein in was found the database. The protein exhibited significant antioxidant activities against free radicals such as DPPH, ABTS and superoxide anion and showed higher activity than ?-tocopherol in a linoleic acid emulsion assay system. Furthermore, GBSP exhibited notable reducing power and a strong chelating effect on Cu(2+) and Fe(2+). Therefore, the present study demonstrates, for the first time, that this novel protein from Ginkgo biloba seeds is an excellent antioxidant. PMID:23519252

Zhou, Hao; Chen, Xijuan; Wang, Chengzhang; Ye, Jianzhong; Chen, Hongxia

2012-12-11

425

Polyphenol Content and Antioxidant Properties of Colored Soybean Seeds from Central Europe  

PubMed Central

Abstract The antioxidant activity and contents of various polyphenol classes in the seeds of seven soybean varieties of different seed color and one yellow seed cultivar, representing a reference genotype, were evaluated. Total polyphenols and tannins were determined after extraction of plant material with 70% aqueous acetone, and total flavonoids were extracted with methanol and acetic acid, whereas anthocyanins were extracted with 20% aqueous ethanol. In addition, isoflavone content and composition were determined using high-performance liquid chromatography analysis. Antioxidant activity of seed extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay. A positive linear correlation between antioxidant activity and contents of total polyphenols and anthocyanins was established. The highest antioxidant activity was observed in the extracts of black and brown varieties, which also showed high levels of all polyphenol classes examined. Yellow seed had the highest total isoflavone content (3.62?mg/g of dry material). The highest concentration of total daidzein was determined in black seeds (>2.0?mg/g of dry material), and the highest total glycitein and genistein contents occurred in the yellow cultivar (0.53 and 1.49?mg/g of dry material, respectively). According to our results, varieties of black and brown seeds could be of special interest not only for their large content of total polyphenols, ranging from 4.94 to 6.22?mg of gallic acid equivalents/g of dry material, but also for their high content of natural antioxidants such as anthocyanins.

Cvejic, Jelena; Miladinovic, Jegor

2012-01-01

426

Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity.  

PubMed

Anthocyanins and proanthocyanidins were characterized by HPLC-ESI-MS/MS coupled with a diode array and/or fluorescent detector in seven cultivars of Ribes nigrum (black currant) and Ribes rubrum (red currant, Red Lake), six cultivars of Ribes grossularia (gooseberries), Aronia melanocarpa(chokeberry), and Sambucus nigra (elderberry). Thirty-one different anthocyanins were detected in these berries, but not every anthocyanin was observed in each berry. A number of minor anthocyanins were identified from these berries for the first time. The concentrations of individual anthocyanins in all of the berries were quantified using relevant anthocyanidin 3-glucoside standards. Among the berries studied in this paper and in berries in general, chokeberry has the highest total anthocyanin concentrations [1480 mg/100 g of fresh weight (FW)], whereas the lowest total anthocyanin concentration in the berries studied was found in the gooseberry cv. Careless, which contained only 0.07 mg/100 g of FW. Two cultivars of gooseberries (Marigold and Leveller) did not contain any anthocyanins. Total proanthocyanidin concentrations in the berries studied ranged from 23 to 664 mg/100 g of FW in elderberry and chokeberry, respectively. Procyanidin or prodelphinidin polymers were the predominant components (>65% w/w) in most of the berries. The lipophilic and hydrophilic antioxidant capacities were measured by the oxygen radical absorbance capacity (ORAC(FL)) procedure. The total antioxidant capacity varied from 21 micromol of TE/g of FW in Careless gooseberry to 161 micromol of TE/g of FW in chokeberry. Total phenolics in the berries in general paralleled hydrophilic antioxidant capacity. PMID:15612766

Wu, Xianli; Gu, Liwei; Prior, Ronald L; McKay, Steve

2004-12-29

427

Antioxidant properties of neohesperidin dihydrochalcone: inhibition of hypochlorous acid-induced DNA strand breakage, protein degradation, and cell death.  

PubMed

Neohesperidin dihydrochalcone (NHDC), a non-nutritive sweetening agent, is simply produced by hydrogenation of neohesperidin. The aim of this study is to evaluate the antioxidant and radical scavenging properties of neohesperidin dihydrochalcone and other structurally related compounds (phloridzin, neohesperidin) toward different reactive radical and oxygen species including .ABTS+, .O2-, .OH, H2O2, and HOCl in vitro. NHDC showed remarkable radical scavenging activity against stable radical and reactive oxygen species (ROS) in concentration dependent manner. Especially, NHDC was the most potent inhibitor of H2O2 and HOCl. NHDC showed HOCl scavenging activity of 93.5% and H2O2 scavenging property of 73.5% which was more than those of all the tested compounds including ascorbic acid and BHT. Moreover, NHDC could inhibit protein degradation, plasmid DNA strand cleavage and HIT-T15, HUVEC cell death from HOCl attack while mannitol, BHT, and ascorbic acid could not protect them effectively. These results suggest that NHDC is a potent antioxidant, especially it is evaluated as a novel HOCl scavenger. This study implies the possibility of therapeutic effect of NHDC on ROS-related inflammatory diseases. PMID:17268074

Choi, Je-Min; Yoon, Byoung-Seok; Lee, Sang-Kyou; Hwang, Jae-Kwan; Ryang, Ryung

2007-02-01

428

Cognitive enhancement in aged mice after chronic administration of Cedrus deodara Loud. and Pinus roxburghii Sarg. with demonstrated antioxidant properties.  

PubMed

Cedrus deodara and Pinus roxburghii, plants mentioned in Indian literature, have been described to possess central nervous system effects and are used in Ayurvedic medicine to treat disorders of the mind. To investigate the memory-enhancing activity of volatile oil and chloroform extracts of C. deodara and P. roxburghii in the Morris water maze paradigm as well as evaluating their antioxidant properties. Aged albino mice were used to study the effect of oil and chloroform extracts on learning and memory by using the Morris water maze paradigm. The chloroform extract of C. deodara produced a significant decrease in escape latency over 7 days of training in both reference and working memory training in comparison to the control