Sample records for characterization antioxidant properties

  1. Characterization of the antioxidant properties of phenolic extracts from some citrus peels

    Microsoft Academic Search

    G. Oboh; A. O. Ademosun

    This study sought to determine the distribution of free and bound phenolics in some Nigerian citrus peels [orange (Citrus sinensis), grapefruit (Citrus paradisii) and shaddock (Citrus maxima)] and characterize the antioxidant properties. The free phenolics were extracted with 80% acetone, while the bound phenolics\\u000a were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Free phenolic extracts had

  2. Partial characterization, antioxidative properties and hypolipidemic effects of oilseed cake of Allanblackia floribunda and Jatropha curcas

    PubMed Central

    2013-01-01

    Background High fat diet is known to induce oxidative stress and abnormal changes in lipid metabolism. Many traditional plants have been shown to possess antioxidant and lipid-lowering activities, improving on oxidative status and lipid profile. In this paper, we characterized and examined the antioxidative properties of the oilseed cake of A. floribunda and J. curcas. We also evaluated their effect on lipid profile in the plasma and liver of experimental rats placed on a high fat diet. Methods For a partial characterization, the qualitative and quantitative analyses of storage proteins, dietary fibre and polyphenol content were evaluated. Four extracts (aqueous, ethanolic, methanolic and 0.1 N HCl) were evaluated for their antioxidant properties and scavenging activities. The effect on lipid profile was evaluated after the administration of the crude extracts to albino rats placed on a high fat diet. Results Our results showed that J. curcas contains 10 times more storage proteins than A. floribunda while A. floribunda contains twice as much total dietary fibre than J. curcas. An evaluation of the different families of storage proteins showed that J. curcas has glutelins as the major storage proteins in its seeds (61.65 mg/g d.m), followed by globulins (25.30 mg/g d.m) and albumins (18.30 mg/g d.m). The electrophoretic analyses revealed a diversity of bands at the level of the different families and for both species. The evaluation of the in vitro antioxidant activities showed that A. floribunda extracts had higher antioxidant properties. Although the composition of A. floribunda and J. curcas oilseed cake are different, they lowered serum triglycerides (TG), total cholesterol (TC) and blood glucose level. Conclusion These results show that the oilseed cake of A. floribunda and J. curcas possess antioxidant properties with an effect on blood glucose level and lipid profile. PMID:24330337

  3. Extraction, characterization and antioxidant property of chitosan from cuttlebone Sepia kobiensis (Hoyle 1885).

    PubMed

    Ramasamy, Pasiyappazham; Subhapradha, Namasivayam; Shanmugam, Vairamani; Shanmugam, Annaian

    2014-03-01

    Chitin was extracted from the cuttlebone of Sepia kobiensis and chitosan was prepared through deacetylation. The chitosan was characterized for its structural, physical and thermal (CHN, DDA, FT-IR, NMR, XRD, Viscometric analysis, SEM and DSC) properties. Further, the chitosan exhibited the antioxidant activity of 50.68-74.36% at 1-10 mg ml(-1) and it also showed the reducing power of 0.28% at 1 mg ml(-1). At 10 mg ml(-1), the chitosan exhibited the scavenging ability of 46.17%, on 1,1-diphenyl-2-picrylhydrazyl radicals, 23.38-73.70% on superoxide radicals at 0.05-1.6 mg ml(-1) and 18.34% to 62.39% (0.1-3.2 mg ml(-1)) on hydroxyl radicals; whereas at 1-10 mg ml(-1) the chelating ability on ferrous ions was calculated as 49.74-73.59%. Based on the potential antioxidant activity, scavenging ability on hydroxyl radicals and chelating abilities on ferrous ions, the chitosan from the cuttlebone of S. kobiensis may not only be used as a potent natural antioxidant but also as a possible food quality enhancer ingredient in the pharmaceutical industry. PMID:24333227

  4. Characterization of the antioxidant properties of hydrophilic and lipophilic extracts of Jute (Corchorus olitorius) leaf.

    PubMed

    Oboh, G; Raddatz, H; Henle, T

    2009-01-01

    Corchorus olitorius (jute) is a native plant of tropical Africa and Asia, and has since spread to Australia, South America and some parts of Europe. Its leafy vegetable is popularly used in soup preparation and folk medicine for the treatment of fever, chronic cystitis, cold and tumours. A comparative study of the antioxidant properties of hydrophilic extract (HE) and lipophilic extract (LE) constituents of the leafy vegetable has been assessed. HE and LE of the leaf were prepared using water and hexane, respectively and their antioxidant properties were determined. HE had a significantly higher (P<0.05) 1,1-diphenyl-2-picrylhydrazyl radical-scavenging ability (aqueous, 9.6-84.4%; hexane, 2.0-20.4%), reducing power (aqueous, 0.67 mmol ascorbic acid equivalent/g; hexane, 0.49 mmol ascorbic acid equivalent/g) and trolox equivalent antioxidant capacity (aqueous, 2.3 mmol/g; hexane, 1.1 mmol/g) than LE; conversely, LE had a significantly higher (P<0.05) OH. scavenging activity (44.5-46.2%) than HE (11.6-32.3%), while there was no significant difference (P>0.05) in their Fe(II) chelating ability (HE, 57.7-66.7%; LE, 56.4-61.1%). The higher 1,1-diphenyl-2-picrylhydrazyl radical-scavenging ability, reducing power and trolox equivalent antioxidant capacity of the hydrophilic extract may be due to its significantly higher (P<0.05) total phenol (630.8 mg/100 g), total flavonoid (227.8 mg/100 g) and non-flavonoid polyphenols (403.0 mg/100 g), and its high ascorbic acid content (32.6 mg/100 g). While the higher OH. scavenging ability of LE may be due to its high total carotenoid content (42.5 mg/100 g). Therefore, the additive/synergistic antioxidant activities of the hydrophilic and lipophilic constituents may contribute to the medicinal properties of C. olitorius leaf. PMID:19391031

  5. Characterization of selenium-enriched mycelia of Catathelasma ventricosum and their antihyperglycemic and antioxidant properties.

    PubMed

    Liu, Yuntao; Li, Caiming; Luo, Xiaohu; Han, Guoquan; Xu, Shude; Niu, Fuge; Hu, Xinjie; Wu, Hejun; Zhang, Huimin

    2015-01-21

    This is the first report concerning the selenium enrichment of Catathelasma ventricosum mycelia. The selenium-containing proteins present in selenium-enriched mycelia (Se-MC) were identified using size-exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS). The selenium-containing amino acids liberated by hydrolysis of these proteins were identified using anion exchange-ICP-MS. Se-MC was found to contain selenoproteins with molecular weights ranging from 1.7 to 60.5 kDa. The main selenium-containing amino acids within them were selenomethionine and selenocysteine. Furthermore, Se-MC possessed excellent antihyperglycemic and antioxidant properties. Se-MC normalized biochemical parameters like insulin level, blood glucose level, body weight, and antioxidant enzyme activity in streptozocin-induced diabetic mice. It also inhibited the ?-amylase and ?-glucosidase activities present in in vitro gastric and intestinal models. In conclusion, Se-MC has the potential to serve as a dietary supplement of selenium, an antioxidant, or an ingredient for the formulation of nutraceuticals. PMID:25536291

  6. Synthesis, characterization, DNA binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin-2-hydroxy benzoyl hydrazone.

    PubMed

    Li, Yong; Yang, Zheng-Yin; Wang, Ming-Fang

    2010-07-01

    A novel Schiff-base ligand (H(5)L), hesperetin-2-hydroxy benzoyl hydrazone, and its copper (II), zinc (II) and nickel (II) complexes (M.H(3)L) [M(II) = Cu, Zn, Ni], have been synthesized and characterized. The ligand and Zn (II) complex exhibit green and blue fluorescence under UV light and the fluorescent properties of the ligand and Zn (II) complex in solid state and different solutions were investigated. In addition, DNA binding properties of the ligand and its metal complexes have been investigated by electronic absorption spectroscopy, fluorescence spectra, ethidium bromide displacement experiments, iodide quenching experiments, salt effect and viscosity measurements. Results suggest that all the compounds bind to DNA via an intercalation binding mode. Furthermore, the antioxidant activity of the ligand and its metal complexes was determined by superoxide and hydroxyl radical scavenging methods in vitro. The metal complexes were found to possess potent antioxidant activity and be better than the free ligand alone and some standard antioxidants like vitamin C and mannitol. PMID:20352308

  7. In vitro antioxidant properties and characterization in nutrients and phytochemicals of six medicinal plants from the Portuguese folk medicine

    Microsoft Academic Search

    Lillian Barros; Sónia Oliveira; Ana Maria Carvalho; Isabel C. F. R. Ferreira

    2010-01-01

    Traditional ethnomedical use of plants is recognized as an important potential source of compounds used in mainstream medicine. Herein, the in vitro antioxidant properties, nutrients and phytochemical composition of six medicinal plants widely used in the north-eastern Portuguese region were evaluated. The antioxidant activity was screened through: radical scavenging effects, reducing power, and inhibition of lipid peroxidation in brain homogenates.

  8. Antioxidant properties of amidothionophosphates: Novel antioxidant molecules

    Microsoft Academic Search

    Oren Tirosh; Yehoshua Katzhendler; Yechezkel B Arenholz; Isaac Ginsburg; Ron Kohen

    1996-01-01

    This work describes the synthesis and characterization of a new family of antioxidants. The molecules have the same active group, but different oil-to-water and octanol-to-water partition coefficients due to different substituents. Three new molecules were synthesized based on the chemical structure of the primary amide attached to a thiophosphate group forming an amidothionophosphate. The amidothionophosphate molecules were exposed to the

  9. Characterization and antioxidant properties of alcoholic extracts from gamma irradiated ?-carrageenan

    NASA Astrophysics Data System (ADS)

    Relleve, Lorna; Abad, Lucille

    2015-07-01

    Different extracts from unirradiated and gamma irradiated ?-carrageenan (solid and 1% w/v aqueous solution) were obtained with isopropyl alcohol (IPA) at concentrations of 40%, 60% and 80% v/v at room temperature. Physical and chemical properties of the different IPA extracts were analyzed by GPC, UV, and FT-IR. The extracts consisted of low molecular weight fragments with an average molecular weight (Mw) ranging from 2300 Da to 5000 Da. UV analyses of extracts from irradiated carrageenan showed varying maximum absorptions in the range of 265-280 nm. FT-IR spectra of all extracts from irradiated carrageenan showed all the important functional groups of carrageenan in the fingerprint region (4000-600 cm-1) and additional carbonyl C=O and C=C double bond peaks. Antioxidant properties of the different extracts were investigated using reducing power assay. The reducing power of extracts from the irradiated solution follows the order of 80%>60%>40% while no trend was observed for all extracts from irradiated solid ?-carrageenan.

  10. [Antioxidant properties of plant extracts].

    PubMed

    Bol'shakova, I V; Lozovskaia, E L; Sapezhinski?, I I

    1998-01-01

    Investigation of antioxidant properties of some plants was carried out. A group of plants exhibited antimicrobial activity was studied in detail. Efficiency of plants as antioxidants was tested by the influence of their extracts on the yield of photochemiluminescence of Gly-Trp solutions. Antioxidant properties were examined under conditions when their own absorption was minimized. Riboflavin as additional sensitizer was used in this experiment for superoxide generation. The antioxidant effect was evaluated with regard to single dose of plant extracts and their concentration in human organism. The effect decreases in the following consequence: Hypericum perforatum > Potentilla erectra > Ledum palustre > Plantago major > Salvia officinalis > Chamomilla recutita > Arctostaphylos uva. PMID:9591094

  11. Biochemical characterization of Helichrysum italicum (Roth) G.Don subsp. italicum (Asteraceae) from Montenegro: phytochemical screening, chemotaxonomy, and antioxidant properties.

    PubMed

    Kladar, Nebojša V; Ana?kov, Goran T; Rat, Milica M; Sr?enovi?, Branislava U; Gruji?, Nevena N; Šefer, Emilia I; Božin, Biljana N

    2015-03-01

    The chemical composition and antioxidant properties of the essential oil and EtOH extract of immortelle (Helichrysum italicum (Roth) G.Don subsp. italicum, Asteraceae) collected in Montenegro were evaluated. The essential oil was characterized by GC/MS analysis, and the content of total phenolics and flavonoids in the EtOH extract was determined using the Folin?Ciocalteu reagent. The free-radical-scavenging capacity (RSC) of both the essential oil and the EtOH extract was assessed with the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) method. Moreover, the inhibition of hydroxyl radical ((.) OH) generation by the EtOH extract of immortelle was evaluated for the first time here. Neryl acetate (28.2%) and ?-curcumene (18.8%) were the main compounds in the essential oil, followed by neryl propionate (9.1%) and ar-curcumene (8.3%). The chemical composition of the oils of the examined and additional 16 selected Helichrysum italicum taxa described in literature were compared using principal component (PCA) and cluster (CA) analyses. The results of the statistical analyses implied the occurrence of at least four different main and three subchemotypes of essential oils. Considering the antioxidant properties, the EtOH extract of immortelle exhibited similar potential as propyl gallate and quercetin, while the essential oil exhibited relatively weak DPPH(.) -scavenging capacity. PMID:25766915

  12. Preliminary Characterization, Antioxidant Properties and Production of Chrysolaminarin from Marine Diatom Odontella aurita

    PubMed Central

    Xia, Song; Gao, Baoyan; Li, Aifen; Xiong, Jihai; Ao, Ziqiang; Zhang, Chengwu

    2014-01-01

    A new chrysolaminarin, named CL2, with a molecular mass of 7.75 kDa, was purified from the marine diatom, Odontella aurita, using DEAE-52 cellulose anion-exchange chromatography and Sephadex G-200 gel-filtration chromatography. The monosaccharide and structural analysis revealed that CL2 was a glucan mainly composed of glucose, which was linked by the ?-d-(1?3) (main chain) and ?-d-(1?6) (side chain) glycosidic bond, demonstrated by infrared spectroscopy (IR) and nuclear magnetic resonance (NMR). The antioxidant activity tests revealed that the CL2 presented stronger hydroxyl radical scavenging activity with increasing concentrations, but less was effective on reducing power analysis and scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The influences of nitrogen concentration and light intensity on chrysolaminarin production of O. aurita were further investigated in a glass column photobioreactor, and a record high chrysolaminarin productivity of 306 mg L?1 day?1 was achieved. In conclusion, the chrysolaminarin CL2 from O. aurita may be explored as a natural antioxidant agent for application in aquaculture, food and pharmaceutical areas. PMID:25251034

  13. Epiafzelechin from the root bark of Cassia sieberiana: detection by DART mass spectrometry, spectroscopic characterization, and antioxidant properties.

    PubMed

    Kpegba, Kafui; Agbonon, Amegnona; Petrovic, Ana G; Amouzou, Etchri; Gbeassor, Messanvi; Proni, Gloria; Nesnas, Nasri

    2011-03-25

    The root bark of Cassia sieberiana was analyzed using direct analysis in real time mass spectrometry, and a main flavonoid component with an [M + H](+) mass of 275 was identified. The flavonoid, epiafzelechin, was isolated and fully characterized with the concerted use of NMR spectroscopy, circular dichroism, and optical rotation. Electronic circular dichroism and optical rotation TDDFT calculations were also performed, and their agreement with the experimental results confirmed the enantiomeric identity of the isolated natural product. The antioxidant activity of the compound was also investigated. PMID:21070009

  14. Antioxidant properties of essential oils

    Microsoft Academic Search

    T. A. Misharina; M. B. Terenina; N. I. Krikunova

    2009-01-01

    By the method of capillary gas-liquid chromatography, we studied the antioxidant properties and stability during the storage\\u000a of hexane solutions of 14 individual essential oils from black and white pepper (Piper nigrum L.), cardamom (Elettaria cardamomum L.), nutmeg (Myristica fragrans Houtt.), mace (Myristica fragrans Houtt), juniper berry (Juniperus communis L.), fennel seed (Foeniculum vulgare Mill., var. dulce Thelling), caraway (Carvum

  15. [Antioxidant properties of essential oils].

    PubMed

    Misharina, T A; Terenina, M B; Krikunova, N I

    2009-01-01

    By the method of capillary gas-liquid chromatography we studied antioxidant properties and stability during the storage of hexane solutions of 14 individual essential oils from black and white pepper (Piper nigrum L.), cardamom (Elettaria cardamomum L.), nutmeg (Myristica fragrans Houtt.), mace (Myristica fragrans Houtt), juniperberry (Juniperus communis L.), seed of fennel (Foeniculum vulgare Mill., var. dulce Thelling), caraway (Carvum carvi L.), dry leaves of cinnamon (Cinnamomum zeylanicum Bl.), marjoram (Origanum majorana L.), laurel (Laurus nobilis L.), ginger (Zingiber officinale L.), garlic (Allium sativum L.), and clove bud (Caryophyllus aromaticus L.). We assessed the antioxidant properties by the oxidation of aliphatic aldehyde (trans-2-hexenal) into the according carbon acid. We established that essential oils of garlic, clove bud, ginger and leaves of cinnamon have the maximal efficiency of inhibition of hexenal oxidation (80-93%), while black pepper oil has the minimal (49%). Antioxidant properties of essential oils with a high content of substituted phenols depended poorly on its concentration in model systems. We studied the changes in essential oils content during the storage of its hexane solutions for 40 days in the light and out of the light and compared it with the stability of essential oils stored for a year out of the light. PMID:20067158

  16. Antioxidant properties of organic sulfur compounds and criteria for evaluating these properties

    Microsoft Academic Search

    I. A. Rubinshtein; E. P. Sobolev

    1965-01-01

    1.The antioxidant properties of organic sulfur compounds are well characterized by the proposed criteria for inhibitor evaluation. These criteria reflect the features of chemical structure of the organic sulfur compounds.2.All of the organic sulfur compounds that were investigated do possess antioxidant properties.3.The antioxidant properties of organic sulfur compounds depend mainly on the nature of the bonds of the sulfur atoms;

  17. Isolation, characterization, spectroscopic properties and quantum chemical computations of an important phytoalexin resveratrol as antioxidant component from Vitis labrusca L. and their chemical compositions

    NASA Astrophysics Data System (ADS)

    Güder, Aytaç; Korkmaz, Halil; Gökce, Halil; Alpaslan, Yelda Bingöl; Alpaslan, Gökhan

    2014-12-01

    In this study, isolation and characterization of trans-resveratrol (RES) as an antioxidant compound were carried out from VLE, VLG and VLS. Furthermore, antioxidant activities were evaluated by using six different methods. Finally, total phenolic, flavonoid, ascorbic acid, anthocyanin, lycopene, ?-carotene and vitamin E contents were carried out. In addition, the FT-IR, 13C and 1H NMR chemical shifts and UV-vis. spectra of trans-resveratrol were experimentally recorded. Quantum chemical computations such as the molecular geometry, vibrational frequencies, UV-vis. spectroscopic parameters, HOMOs-LUMOs energies, molecular electrostatic potential (MEP), natural bond orbitals (NBO) and nonlinear optics (NLO) properties of title molecule have been calculated by using DFT/B3PW91 method with 6-311++G(d,p) basis set in ground state for the first time. The obtained results show that the calculated spectroscopic data are in a good agreement with experimental data.

  18. Cultivated strains of Agaricus bisporus and A. brasiliensis: chemical characterization and evaluation of antioxidant and antimicrobial properties for the final healthy product--natural preservatives in yoghurt.

    PubMed

    Stojkovi?, Dejan; Reis, Filipa S; Glamo?lija, Jasmina; ?iri?, Ana; Barros, Lillian; Van Griensven, Leo J L D; Ferreira, Isabel C F R; Sokovi?, Marina

    2014-07-25

    Agaricus bisporus (J. E. Lange) Emil J. Imbach and Agaricus brasiliensis Wasser, M. Didukh, Amazonas & Stamets are edible mushrooms. We chemically characterized these mushrooms for nutritional value, hydrophilic and lipophilic compounds. The antioxidant and antimicrobial activities of methanolic and ethanolic extracts were assessed. Hepatotoxicity was also evaluated. The ethanolic extract of both species was tested for inhibition of Listeria monocytogenes growth in yoghurt. Both species proved to be a good source of bioactive compounds. A. brasiliensis was richer in polyunsaturated fatty acids and revealed the highest concentration of phenolic acids, and tocopherols. A. bisporus showed the highest monounsaturated fatty acids and ergosterol contents. A. brasiliensis revealed the highest antioxidant potential, and its ethanolic extract displayed the highest antibacterial potential; the methanolic extract of A. bisporus revealed the highest antifungal activity. A. brasiliensis possessed better preserving properties in yoghurt. PMID:24881564

  19. Antitrypanosomal and antioxidant properties of 4-hydroxycoumarins derivatives.

    PubMed

    Pérez-Cruz, Fernanda; Serra, Silvia; Delogu, Giovanna; Lapier, Michel; Maya, Juan Diego; Olea-Azar, Claudio; Santana, Lourdes; Uriarte, Eugenio

    2012-09-01

    In the present communication we prepared a series of six 4-hydroxycoumarin derivatives, isosters of quercetin, recognized as an antioxidant natural compound, with the aim of evaluating the antitrypanosomal activity against Trypanosoma cruzi, the parasite responsible for Chagas disease, and the antioxidant properties. We have used the 4-hydroxycoumarin moiety (compound 1) as the molecular template for the synthesis of compounds 2-7. These derivates have shown moderate trypanocidal activity. However they have been proved to be good antioxidants. In particular compound 7 is the most active antioxidant and it is, therefore, a potential candidate for a successful employment in conditions characterized by free radicals overproduction. PMID:22832320

  20. Phytochemical Characterization of Chinese Bayberry (Myrica rubra Sieb. et Zucc.) of 17 Cultivars and Their Antioxidant Properties

    PubMed Central

    Zhang, Xianan; Huang, Huizhong; Zhang, Qiaoli; Fan, Fangjuan; Xu, Changjie; Sun, Chongde; Li, Xian; Chen, Kunsong

    2015-01-01

    In order to fully understand the variations of fruit quality-related phytochemical composition in Chinese bayberry (Myrica rubra Sieb. et Zucc.), mature fruit of 17 cultivars from Zhejiang and Jiangsu provinces was used for the investigation of fruit quality attributes, including fruit color, soluble sugars, organic acids, total phenolics, flavonoids, antioxidant capacity, etc. Sucrose was the main soluble sugar, while citric acid was the main organic acid in bayberry fruit. The content of total phenolics and total flavonoids were positively correlated with 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) antioxidant activity and 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity. Five anthocyanidins, i.e., delphinidin–hexoside (Dp–Hex), cyanidin-3–O-galactoside (C-3–Gal), cyanidin-3–O-glucoside (C-3–Glu), pelargonidin-3–O-glucoside (Pg-3–Glu) and peonidin-3-O-glucoside (Pn-3–Glu), and seven flavonols compounds, i.e., myricetin-3-O-rhamnoside (M-3–Rha), myricetin deoxyhexoside–gallate (M-DH–G), quercetin-3-O-galactoside (Q-3–Gal), quercetin-3–O-glucoside (Q-3–Glu), quercetin-3–O-rhamnoside (Q-3–Rha), kaempferol-3–O-galactoside (K-3–Gal) and kaempferol-3–O-glucoside (K-3–Glu), were identified and characterized among the cultivars. The significant differences in phytochemical compositions among cultivars reflect the diversity in bayberry germplasm, and cultivars of good flavor and/or rich in various health-promoting phytochemicals are good candidates for future genetic breeding of bayberry fruit of high quality. In conclusion, our results may provide important information for further breeding or industrial utilization of different bayberry resources. PMID:26042467

  1. Phytochemical Characterization of Chinese Bayberry (Myrica rubra Sieb. et Zucc.) of 17 Cultivars and Their Antioxidant Properties.

    PubMed

    Zhang, Xianan; Huang, Huizhong; Zhang, Qiaoli; Fan, Fangjuan; Xu, Changjie; Sun, Chongde; Li, Xian; Chen, Kunsong

    2015-01-01

    In order to fully understand the variations of fruit quality-related phytochemical composition in Chinese bayberry (Myrica rubra Sieb. et Zucc.), mature fruit of 17 cultivars from Zhejiang and Jiangsu provinces was used for the investigation of fruit quality attributes, including fruit color, soluble sugars, organic acids, total phenolics, flavonoids, antioxidant capacity, etc. Sucrose was the main soluble sugar, while citric acid was the main organic acid in bayberry fruit. The content of total phenolics and total flavonoids were positively correlated with 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) antioxidant activity and 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) (ABTS) radical scavenging activity. Five anthocyanidins, i.e., delphinidin-hexoside (Dp-Hex), cyanidin-3-O-galactoside (C-3-Gal), cyanidin-3-O-glucoside (C-3-Glu), pelargonidin-3-O-glucoside (Pg-3-Glu) and peonidin-3-O-glucoside (Pn-3-Glu), and seven flavonols compounds, i.e., myricetin-3-O-rhamnoside (M-3-Rha), myricetin deoxyhexoside-gallate (M-DH-G), quercetin-3-O-galactoside (Q-3-Gal), quercetin-3- O-glucoside (Q-3-Glu), quercetin-3-O-rhamnoside (Q-3-Rha), kaempferol-3-O-galactoside (K-3-Gal) and kaempferol-3-O-glucoside (K-3-Glu), were identified and characterized among the cultivars. The significant differences in phytochemical compositions among cultivars reflect the diversity in bayberry germplasm, and cultivars of good flavor and/or rich in various health-promoting phytochemicals are good candidates for future genetic breeding of bayberry fruit of high quality. In conclusion, our results may provide important information for further breeding or industrial utilization of different bayberry resources. PMID:26042467

  2. Characterization of microalgal carotenoids by mass spectrometry and their bioavailability and antioxidant properties elucidated in rat model.

    PubMed

    Ranga Rao, A; Raghunath Reddy, R L; Baskaran, V; Sarada, R; Ravishankar, G A

    2010-08-11

    Of the total carotenoids in respective algal samples, beta-carotene in Spirulina platensis was 69.5%, astaxanthin and its esters in Haematococcus pluvialis was 81.38%, and lutein in Botryococcus braunii was 74.6%. The carotenoids were characterized by mass spectrometry. A time-course study of carotenoids in rats after administration of microalgal biomass showed peak levels in plasma, liver, and eyes at 2, 4, and 6 h, respectively. Beta-carotene accumulation in Spirulina-fed rats was maximum in eye tissues at 6 h. Similarly, levels of astaxanthin and lutein in Haematococcus- and Botryococcus-fed rats were also maximal in eye tissues. Astaxanthin from H. pluvialis showed better bioavailability than beta-carotene and lutein. The antioxidant enzymes, catalase, superoxide dismutase, peroxidase, and TBARS were significantly high in plasma at 2 h and in liver at 4 h, evidently offering protection from free radicals. This study implies that microalgae can be a good source of carotenoids of high bioavailability and nutraceutical value. PMID:20681642

  3. Antioxidant properties of myocardial fuels

    Microsoft Academic Search

    Robert T. Mallet; Jie Sun

    2003-01-01

    Oxidative metabolism of blood-borne fuels provides myocardium the energy required to sustain its contractile performance. Recent research has revealed that, in addition to supplying energy, certain fuels are able to detoxify harmful oxidants and bolster the myocardium's endogenous antioxidant defenses. These antioxidant capabilities could potentially protect the myocardium from the ravages of reactive oxygen and nitrogen intermediates generated upon reperfusion

  4. Structural characterization, antioxidant and anticancer properties of gold nanoparticles synthesized from leaf extract(decoction)of Antigonon leptopus Hook. &Arn.

    PubMed

    Balasubramani, Govindasamy; Ramkumar, Rajendiran; Krishnaveni, Narayanaswamy; Pazhanimuthu, Annamalai; Natarajan, Thillainathan; Sowmiya, Rajamani; Perumal, Pachiappan

    2015-04-01

    Tea is an aromatic beverage prepared by pouring boiling water over alleviated leaves of the tea plant. Tea prepared from the aerial parts of Antigonon leptopus has been traditionally used as remedy for cold, diabetes and pain in many countries. The gold nanoparticles (Au NPs) synthesized from powdered leaf extract (decoction) of A. leptopus were characterized by UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier transform-infrared (FT-IR), high resolution transmission electron microscopy (HR-TEM), selected area electron diffraction (SAED) pattern and energy dispersive X-ray (EDX) analyses to define the formation of Au NPs. Further, the synthesized Au NPs were well characterized based on their strong surface plasmon resonance (SPR), crystalline nature, functional groups, size and dispersed shapes, purity and Bragg's reflections of face centered cubic (fcc) structure of metallic gold. The Au NPs showed higher free radical scavenging property when compared to the effect of leaf extract. Cytotoxicity study of synthesized Au NPs exhibited the growth inhibitory property at the concentration (GI50) of 257.8 ?g/mL in human adenocarcinoma breast cancer (MCF-7) cells after 48 h. Thus, the Au NPs synthesized from the Mexican creeper, A. leptopus revealed the important biological properties: as a free radical as well as anticancer agent. We conclude that the A. leptopus derived biological materials have promising potential as a source for the development of anticancer drug in future. PMID:25432487

  5. Antioxidant properties of several specialty mushrooms

    Microsoft Academic Search

    Jeng-Leun Mau; Hsiu-Ching Lin; Si-Fu Song

    2002-01-01

    Four specialty mushrooms are commercially available in Taiwan, including Dictyophora indusiata (basket stinkhorn), Grifola frondosa (maitake), Hericium erinaceus (lion's mane), and Tricholoma giganteum (white matsutake). Methanolic extracts were prepared from these specialty mushrooms and their antioxidant properties were studied. The antioxidant activities at 1.2 mg ml?1 were in the order of basket stinkhorn>lion's mane>maitake>white matsutake. Basket stinkhorn showed an excellent

  6. Morphological and genetic characterization of a cultivated Cordyceps sinensis fungus and its polysaccharide component possessing antioxidant property in H22 tumor-bearing mice

    Microsoft Academic Search

    Jiaping Chen; Weiyun Zhang; Tingting Lu; Jing Li; Yi Zheng; Lingdong Kong

    2006-01-01

    Cordyceps sinensis, one of the most precious traditional Chinese medicines, possesses the antitumor activity, antioxidant activity and the capability of modulating the immune system. In the present study, a fungus strain G1 isolated from wild C. sinensis was identified and initially characterized. A phylogenetic tree was generated based on the sequences of the internal transcribed spacer (ITS) region of related

  7. [Antioxidant and membranoprotective properties of trolox].

    PubMed

    Tibir'kova, E V; Kosolapov, V A; Spasov, A A

    2009-01-01

    The antioxidant and membranoprotective properties of trolox have been studied in vitro. It is established that trolox possesses antioxidant properties, inhibiting ascorbate-dependent lipid peroxidation of liver homogenates and Fe(2+)-induced lipid chemiluminescence of egg-yolk lipids. In addition, trolox exhibits a broad spectrum of antiradical activity and interacts with stable free 2,2-diphenyl-1-picrylhydrazyl radical so as to reduce the generation of luminol radicals and reactive oxygen species and to bind peroxy radicals. Trolox also possessed membranoprotective properties, being capable of reducing the oxidative hemolysis of erythrocytes. PMID:19441730

  8. Pectic polysaccharide from the green fruits of Momordica charantia (Karela): structural characterization and study of immunoenhancing and antioxidant properties.

    PubMed

    Panda, Bibhash C; Mondal, Soumitra; Devi, K Sanjana P; Maiti, Tapas K; Khatua, Somanjana; Acharya, Krishnendu; Islam, Syed S

    2015-01-12

    A water soluble pectic polysaccharide (PS) isolated from the aqueous extract of the green fruits of Momordica charantia contains D-galactose and D-methyl galacturonate in a molar ratio of nearly 1:4. It showed splenocyte, thymocyte as well as macrophage activations. Moreover, it exhibited potent antioxidant activities. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and 1D and 2D NMR studies, the structure of the repeating unit of the pectic polysaccharide was established as: [Formula: see text]. PMID:25464078

  9. Potential Anticancer Properties of Grape Antioxidants

    PubMed Central

    Zhou, Kequan; Raffoul, Julian J.

    2012-01-01

    Dietary intake of foods rich in antioxidant properties is suggested to be cancer protective. Foods rich in antioxidant properties include grape (Vitis vinifera), one of the world's largest fruit crops and most commonly consumed fruits in the world. The composition and cancer-protective effects of major phenolic antioxidants in grape skin and seed extracts are discussed in this review. Grape skin and seed extracts exert strong free radical scavenging and chelating activities and inhibit lipid oxidation in various food and cell models in vitro. The use of grape antioxidants are promising against a broad range of cancer cells by targeting epidermal growth factor receptor (EGFR) and its downstream pathways, inhibiting over-expression of COX-2 and prostaglandin E2 receptors, or modifying estrogen receptor pathways, resulting in cell cycle arrest and apoptosis. Interestingly, some of these activities were also demonstrated in animal models. However, in vivo studies have demonstrated inconsistent antioxidant efficacy. Nonetheless, a growing body of evidence from human clinical trials has demonstrated that consumption of grape, wine and grape juice exerts many health-promoting and possible anti-cancer effects. Thus, grape skin and seed extracts have great potential in cancer prevention and further investigation into this exciting field is warranted. PMID:22919383

  10. Antioxidant and Prooxidant Properties of Carotenoids

    Microsoft Academic Search

    Andrew J. Young; Gordon M. Lowe

    2001-01-01

    The ability of dietary carotenoids such as ?-carotene and lycopene to act as antioxidants in biological systems is dependent upon a number of factors. While the structure of carotenoids, especially the conjugated double bond system, gives rise to many of the fundamental properties of these molecules, it also affects how these molecules are incorporated into biological membranes. This, in turn,

  11. Tunisian date (Phoenix dactylifera L.) by-products: Characterization and potential effects on sensory, textural and antioxidant properties of dairy desserts.

    PubMed

    Jridi, Mourad; Souissi, Nabil; Salem, Marwa Ben; Ayadi, M A; Nasri, Moncef; Azabou, Samia

    2015-12-01

    Three Tunisian date varieties, Deglet Nour, Kentichi and Allig, served to produce syrups and powders, which were then examined for their physico-chemical composition and antioxidant properties. Different proportions of these sweetening-like agents were incorporated to produce nine different formulations of dairy desserts, with lower amount of added sugars to avoid any artificial flavoring or coloring agents. Sensory and color evaluation data revealed that incorporating Deglet Nour and Kentichi syrup offers the most desirable formulation. Furthermore, syrup polysaccharides and fibers contribute to better maintain the final product texture. In addition, date by-products create a good source of natural thickening agents, involved in enhancing apparent viscosity and spontaneous exudation. Thanks to their high content in phenolic compounds, date by-products considerably improve antioxidant activities of the formulated desserts. Therefore, they could be valued as natural ingredients in the formulation of novel dairy products with high nutritional-properties. PMID:26041157

  12. Antioxidant properties of hydroxy-flavones

    Microsoft Academic Search

    Nicole Cotelle; Jean-Luc Bernier; Jean-Pierre Catteau; Jean Pommery; Jean-Claude Wallet; Emile M. Gaydou

    1996-01-01

    The antioxidant properties of 24 hydroxy-flavones were evaluated. Results show that 2?,3?,4?-OH substitution on the B ring plays a crucial role in radical scavenger activity in the DPPH assay and in the inhibitory effect on pereoxydation of tissue lipids in the MDA test. The formation of stable radicals for this type of compounds has been studied by ESR. In addition,

  13. Extensive screening for herbal extracts with potent antioxidant properties

    PubMed Central

    Niwano, Yoshimi; Saito, Keita; Yoshizaki, Fumihiko; Kohno, Masahiro; Ozawa, Toshihiko

    2011-01-01

    This paper summarizes our research for herbal extracts with potent antioxidant activity obtained from a large scale screening based on superoxide radical (O2•?) scavenging activity followed by characterization of antioxidant properties. Firstly, scavenging activity against O2•? was extensively screened from ethanol extracts of approximately 1000 kinds of herbs by applying an electron spin resonance (ESR)-spin trapping method, and we chose four edible herbal extracts with prominently potent ability to scavenge O2•?. They are the extracts from Punica granatum (Peel), Syzygium aromaticum (Bud), Mangifera indica (Kernel), and Phyllanthus emblica (Fruit). These extracts were further examined to determine if they also scavenge hydroxyl radical (•OH), by applying the ESR spin-trapping method, and if they have heat resistance as a desirable characteristic feature. Experiments with the Fenton reaction and photolysis of H2O2 induced by UV irradiation demonstrated that all four extracts have potent ability to directly scavenge •OH. Furthermore, the scavenging activities against O2•? and •OH of the extracts of P. granatum (peel), M. indica (kernel) and P. emblica (fruit) proved to be heat-resistant. The results of the review might give useful information when choosing a potent antioxidant as a foodstuff. For instance, the four herbal extracts chosen from extensive screening possess desirable antioxidant properties. In particular, the extracts of the aforementioned three herbs are expected to be suitable for food processing in which thermal devices are used, because of their heat resistance. PMID:21297917

  14. Synthesis and evaluation of antioxidant and antimicrobial properties of thymol containing pyridone moieties

    Microsoft Academic Search

    P. S. Nagle; Y. A. Pawar; A. E. Sonawane; S. M. Bhosale; D. H. More

    Various derivatives of 1,2-dihydro-6-(4-hydroxy-5-isopropyl-2-methylphenyl)-2-oxo-4-arylpyridine-3-carbonitrile (IVa–m) were synthesized and characterized. Their antioxidant and antimicrobial properties were evaluated by measuring their activity\\u000a in vitro. Among the newly synthesized derivatives IVg, IVh, IVi, IVj, IVk, IVl, and IVm showed good antioxidant activity. However, all derivatives show better antibacterial and antifungal properties than that\\u000a of parent thymol. The probable mechanism, particularly for antioxidant properties

  15. Characterization of antioxidants and change of antioxidant levels during storage of Manilkara zapota L.

    PubMed

    Shui, Guanghou; Wong, Shih Peng; Leong, Lai Peng

    2004-12-29

    Antioxidants found in fruits and vegetables play an important role via their protective effects against the onset of aging-related chronic diseases. Our previous research has indicated that unripe ciku fruits (Manilkara zapota L.) are an excellent source of antioxidants, with over 3000 mg of L-ascorbic acid equivalent antioxidant capacity (AEAC) per 100 g of fresh sample. In this study, 24 antioxidants in an extract of ciku king were characterized through a free radical spiking test. Their chemical structures were proposed using high-performance liquid chromatography-mass spectrometry (HPLC-MS) and tandem MS (HPLC/MSn). The antioxidant capacity of ciku king fruits was mainly attributed to polyphenolics with basic blocks of gallocatechin or catechin or both. The changes of total antioxidant capacity (TAC) and total phenolics content (TPC) of ciku king fruits with storage time were also investigated. It was found that the TAC and TPC decreased significantly as the fruits gradually changed from the unripe to the overripe stage. The best time for one to consume ciku king fruits at a flavorful stage with high amounts of antioxidants with AEAC values ranging from 600 to 1200 mg per 100 g fresh sample is suggested. The change of the content of major antioxidant peaks was also consistent with changes of antioxidant levels during storage. PMID:15612764

  16. Antioxidant and cytoprotective properties of partridgeberry polyphenols.

    PubMed

    Bhullar, Khushwant S; Rupasinghe, H P Vasantha

    2015-02-01

    Partridgeberry (Vaccinium vitis-idaea) is a polyphenol-rich berry of the Ericaceous family, grown in Newfoundland and Labrador province of Canada. The aims of this study were to identify extraction solvents for the maximum recovery of polyphenols, to establish fractionation technique for isolation of major sub-classes of polyphenols, and to evaluate antioxidant and cytoprotective properties of the partridgeberry polyphenol preparations. The acidified 70% acetone was identified as the ideal solvent for the maximum recovery of polyphenols from partridgeberry. Further, aqueous two-phase extraction, column chromatography and UPLC-MS/MS were employed to produce three partridgeberry polyphenol fractions, rich in either, anthocyanins, flavan-3-ols or flavonols. All the three PPF were potent antioxidants and displayed cytoprotective activity through the activation of nuclear factor erythroid 2-related factor 2 pathway, scavenging of reactive oxygen species, and inhibition of cellular death. The current study suggests that partridgeberry has numerous potential health implications in both prevention and amelioration of various diseases involving oxidative stress. PMID:25172753

  17. Chemical properties and antioxidant and antimicrobial activities of Slovenian propolis.

    PubMed

    Mavri, Ana; Abramovi?, Helena; Polak, Tomaž; Bertoncelj, Jasna; Jamnik, Polona; Smole Možina, Sonja; Jeršek, Barbara

    2012-08-01

    The chemical composition as well as the antioxidant and antimicrobial activities of two EtOH extracts of propolis (PEEs) from Slovenia were determined. EtOH was used as extracting solvent at 70 and 96%, providing the extracts PEE70 and PEE96, respectively. The extraction with 70% EtOH was more efficient than that with 96% EtOH, as the PEE70 was richer in total phenolic compounds than the PEE96. The Slovenian propolis was characterized by different phenolic acids and flavonoids. The PEE96 was slightly richer in three specific compounds, i.e., caffeic acid, ferulic acid, and luteolin, while all other substances detected showed higher contents in the PEE70. The PEE70 showed a stronger reducing power and ability to scavenge free radicals and metal ions than the PEE96. Both PEEs were in the main more effective against Gram-positive bacteria than against fungi and Gram-negative bacteria like Salmonella and Escherichia coli, with the exception of Campylobacter. The PEE96 decreased the intracellular oxidation in Saccharomyces cerevisiae in a dose-dependent manner. The antimicrobial activities and antioxidant properties were related to the total phenolic contents. The two PEEs have the potential for use as natural antimicrobial and antioxidant additives in foods. PMID:22899615

  18. Preliminary structural characterization and antioxidant activities of polysaccharides extracted from Hawk tea (Litsea coreana var. lanuginosa).

    PubMed

    Jia, Xuejing; Dong, Lihua; Yang, Yong; Yuan, Shu; Zhang, Zhongwei; Yuan, Ming

    2013-06-01

    Three polysaccharides were extracted from different leaf age Hawk teas (Litsea coreana var. lanuginosa) by hot water method. Preliminary structural characterization was conducted by physicochemical property, fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analyses. Antioxidant activities against 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ferric reducing activity power (FRAP), hydroxyl radical and erythrocyte hemolysis were also evaluated. The physicochemical property analysis indicated significant differences in the three polysaccharides. The FTIR spectra revealed the general characteristic absorption peaks of the three polysaccharides. The SEM images demonstrated significant differences in the surface features of the different polysaccharides. The antioxidant activity assays revealed the obvious antioxidant activities of three polysaccharides, and the polysaccharides of Hawk primary leaf tea exhibited higher antioxidant activities than the other two polysaccharides. With current findings, the polysaccharides from Hawk primary leaf tea may have potential applications in food industries. PMID:23618259

  19. Boldine and its antioxidant or health-promoting properties.

    PubMed

    O'Brien, Peter; Carrasco-Pozo, Catalina; Speisky, Hernán

    2006-01-01

    The increasing recognition of the participation of free radical-mediated oxidative events in the initiation and/or progression of cardiovascular, tumoural, inflammatory and neurodegenerative disorders, has given rise to the search for new antioxidant molecules. An important source of such molecules has been plants for which there is an ethno-cultural base for health promotion. An important example of this is boldo (Peumus boldus Mol.), a chilean tree whose leaves have been traditionally employed in folk medicine and is now widely recognized as a herbal remedy by a number of pharmacopoeias. Boldo leaves are rich in several aporphine-like alkaloids, of which boldine is the most abundant one. Research conducted during the early 1990s led to the discovery that boldine is one of the most potent natural antioxidants. Prompted by the latter, a large and increasing number of studies emerged, which have focused on characterizing some of the pharmacological properties that may arise from the free radical-scavenging properties of boldine. The present review attempts to exhaustively cover and discuss such studies, placing particular attention on research conducted during the last decade. Mechanistic aspects and structure-activity data are discussed. The review encompasses pharmacological actions, which arise from its antioxidant properties (e.g., cyto-protective, anti-tumour promoting, anti-inflammatory, anti-diabetic and anti-atherogenic actions), as well as those that do not seem to be associated with such activity (e.g., vasorelaxing, anti-trypanocidal, immuno- and neuro-modulator, cholagogic and/or choleretic actions). Based on the pharmacological and toxicological data now available, further research needs and recommendations are suggested to define the actual potential of boldine for its use in humans. PMID:16221469

  20. Comparison of the Antioxidant Properties of Commonly Consumed Commercial Teas

    Microsoft Academic Search

    Anna Pekal; Paulina Drozdz; Krystyna Pyrzynska

    2012-01-01

    The antioxidant properties of various commonly consumed commercial teas were screened and compared to check what the consumers get in commercial tea bags in the range of consume preparation conditions. It includes flavored black teas and green teas, as well as some fruit teas. Electron transfer-based assays, such as phenolic content by Folin-Ciocalteu method and cupric ion reducing antioxidant capacity

  1. COMPARISON OF THE ANTIOXIDANT PROPERTIES OF COMMONLY CONSUMED COMMERCIAL TEAS

    Microsoft Academic Search

    Anna Pekal; Paulina Drozdz; Krystyna Pyrzynska

    2011-01-01

    The antioxidant properties of various commonly consumed commercial teas were screened and compared to check what the consumers get in commercial tea bags in the range of consume preparation conditions. It includes flavored black teas, green teas as well as some fruit teas. Electron transfer-based assays such as phenolic content by Folin-Ciocalteu method and cupric ion reducing antioxidant capacity (CUPRAC)

  2. In vitro antioxidant properties of mangosteen peel extract.

    PubMed

    Suttirak, Weerayuth; Manurakchinakorn, Supranee

    2014-12-01

    The growing interest in the replacement of synthetic food antioxidants by natural ones has fostered research on the screening of plant-derived raw materials for identifying new antioxidants. The special attention of research today is focused on inexpensive or residual sources from agricultural industries. Fruit peels as sources of powerful natural antioxidants are often the waste parts of various fruits from consumption and food industry. Among the fruit peels, mangosteen peel is an important source of natural phenolic antioxidants. The mangosteen peel contains various bioactive substances, i.e., phenolic acids and flavonoids, which possess biological and medicinal properties, especially antioxidant properties. The aim of this review, after presenting analytical techniques for determining in vitro antioxidant activity of mangosteen peel extract, is to summarize available data on the factors affecting antioxidant activity of mangosteen peel extract. In addition, the potential antioxidant activity of mangosteen peel extract, the bioactive compounds identified from mangosteen peel extract and their antioxidant activity are presented. Potential applications of the mangosteen peel extract in food, pharmaceutical and cosmetic products are also discussed. PMID:25477623

  3. Antioxidant properties of some lichen species.

    PubMed

    Kosani?, Marijana; Rankovi?, Branislav; Vukojevi?, Jelena

    2011-10-01

    Antioxidant activity of the acetone, methanol and aqueous extracts of the lichens Cladonia furcata, Hypogymnia physodes, Lasallia pustulata, Parmelia caperata and Parmelia sulcata has been screened in vitro by using different methods (DPPH radical scavenging, superoxide anion radical scavenging, reducing power, determination of total phenolic compounds and determination of total flavonoid content). Of the lichens tested, Lasallia pustulata had powerful antioxidant activities. Acetone, methanol and aqueous extracts of this lichen showed 90.93, 69.87 and 65.08% DPPH radical scavenging activities. Moreover, the tested extracts had effective reducing power and superoxide anion radical scavenging. Those various antioxidant activities were compared to standard antioxidants such as ascorbic acid, butylated hydroxyanisole (BHA) and ?-tocopherol. In addition, total content of phenol and flavonoid in extracts were determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The strong relationships between total phenolic and flavonoid contents and the antioxidative activities of tested extracts suggest that these compounds play important role in antioxidant activity. The present stady shows that tested lichen species demonstrated a strong antioxidant activity and can be considered as good sources of natural antioxidants. PMID:23572791

  4. Antioxidant properties of anthocyanidins, anthocyanidin-3-glucosides and respective portisins

    Microsoft Academic Search

    Joana Azevedo; Iva Fernandes; Ana Faria; Joana Oliveira; Ana Fernandes; Victor de Freitas; Nuno Mateus

    2010-01-01

    The study of the antioxidants properties of three classes of pigments, namely anthocyanidins (cyanidin-Cy, delphinidin-Dp and malvidin-Mv), anthocyanidin-3-glucosides (Cy-3-gluc, Dp-3-gluc and Mv-3-gluc), and portisins (Cy-pt, Dp-pt and Mv-pt) was carried out. The aim was to evaluate the relationship between the structure antioxidant properties of individual anthocyanins and respective derivative pigments. The ability of these compounds to inhibit lipid peroxidation in

  5. Antioxidant properties of tea investigated by EPR spectroscopy

    Microsoft Academic Search

    Martin Polovka; Vlasta Brezová; Andrej Staško

    2003-01-01

    The antioxidant properties of green, black and mixed (fruit) tea samples of different origin were investigated by means of EPR spectroscopy. A six line EPR spectrum of solid tea samples indicates the presence of Mn(II) ions and it is superimposed with a sharp singlet line attributed to semiquinone radical species (?Hpp=1 mT; g=2.0022). Antioxidant properties of aqueous tea extracts in

  6. Antioxidant properties of carboxymethyl glucan: comparative analysis.

    PubMed

    Babincová, M; Bacová, Z; Machová, E; Kogan, G

    2002-01-01

    Antioxidative capabilities of carboxymethylated (1 --> 3)-beta-D-glucan from Saccharomyces cerevisiae cell wall, alpha-tocopherol, and mannitol against lipid peroxidation in phosphatidylcholine liposomes induced by OH. radicals produced with Fenton's reagent (H(2)O(2)/Fe(2+)) were studied using absorption ultraviolet-visible spectrophotometry. It was found that (1 --> 3)-beta-D-glucan is an antioxidant with the scavenging ability lying between that of alpha-tocopherol, which is known to be incorporated in the lipid bilayer, and the water-soluble antioxidant, mannitol. PMID:12487754

  7. Antioxidant characterization of some Sicilian edible wild greens.

    PubMed

    Salvatore, Sara; Pellegrini, Nicoletta; Brenna, Oreste V; Del Rio, Daniele; Frasca, Graziella; Brighenti, Furio; Tumino, Rosario

    2005-11-30

    Epidemiological studies have demonstrated that many antioxidants and the total antioxidant capacity (TAC) of the diet may protect against cancers and cardiovascular disease. Common fruits and vegetables are good sources of antioxidants, although in some Mediterranean areas traditional wild greens are responsible for a significant percentage of total dietary antioxidant intake. In the European Prospective Investigation into Cancer and Nutrition cohort of Ragusa (Sicily), a high number of subjects were found to frequently eat wild greens, including Sinapis incana and Sinapis nigra, Diplotaxis erucoides, Cichorium intybus, Asparagus acutifolius, and Borrago officinalis. On the basis of these observations, detailed characterization of single antioxidant components (i.e., polyphenols, carotenoids, chlorophylls, and ascorbic acid) and the TAC of these edible wild traditional plants was performed. The wild plants examined were found to be very rich in antioxidants, such as flavonoids and carotenoids, with high TAC values, suggesting that the importance of these vegetables, not only in the traditional but even in the contemporary diet, needs to be emphasized. PMID:16302763

  8. Antioxidant properties of some hydroalcoholic plant extracts with antiinflammatory activity.

    PubMed

    Herold, Aurora; Cremer, Lidia; Calug?ru, Ana; Tama?, Viorica; Ionescu, F; Manea, S; Szegli, G

    2003-01-01

    The hydroalcoholic extracts of Calendula officinalis, Hypericum perforatum, Plantago lanceolata and Glycyrrhiza glabra which exhibited different anti-inflammatory activities were evaluated for the possible mode of action by studying their antioxidant potential. In the present study we investigated if standardized hydroalcoholic extracts of plants such as Calendula officinalis, Hypericum perforatum, Plantago lanceolata and Glycyrrhiza glabra produced by Hofigal Stock Company could modulate the respiratory burst of human activated neutrophils, as a consequence of their antioxidant capacity. Their antioxidant properties were measured using a colorimetric assay (Total Antioxidant Status kit). We demonstrated that Hypericum perforatum and Calendula officinalis hydroalcoholic extracts possessed a significant antioxidant activity while Plantago lanceolata and Glycyrrhiza glabra hydroalcoholic extracts had a minor antioxidant status. Using reactive oxygen species-generating systems (OZ-activated human PMN neutrophils), Calendula officinalis and Hypericum perforatum extracts showed strong reactive oxygen species scavenging property, Hypericum perforatum extract exhibing the highest scavenging activity. These results confirm the potential of Calendula officinalis and Hypericum perforatum investigated hydroalcoholic extracts as medicinal remedies to be used in different inflammatory/allergic diseases. These extracts could be a useful tool for obtaining new antioxidant/anti-inflammatory agents. PMID:16008145

  9. Antioxidant properties of roasted coffee residues.

    PubMed

    Yen, Wen-Jye; Wang, Bor-Sen; Chang, Lee-Wen; Duh, Pin-Der

    2005-04-01

    The antioxidant activity of roasted coffee residues was evaluated. Extraction with four solvents (water, methanol, ethanol, and n-hexane) showed that water extracts of roasted coffee residues (WERCR) produced higher yields and gave better protection for lipid peroxidation. WERCR showed a remarkable protective effect on oxidative damage of protein. In addition, WERCR showed scavenging of free radicals as well as the reducing ability and to bind ferrous ions, indicating that WERCR acts as both primary and secondary antioxidants. The HPLC analyses showed that phenolic acids (chlorogenic acid and caffeic acid) and nonphenolic compounds [caffeine, trigonelline, nicotinic acid, and 5-(hydroxymethyl)furfuraldehyde] remained in roasted coffee residues. These compounds showed a protective effect on a liposome model system. The concentrations of flavonoids and polyphenolic compounds in roasted coffee residues were 8,400 and 20,400 ppm, respectively. In addition, the Maillard reaction products (MRPs) remaining in roasted coffee residues were believed to show antioxidant activity. These data indicate that roasted coffee residues have excellent potential for use as a natural antioxidant source because the antioxidant compounds remained in roasted coffee residues. PMID:15796608

  10. Antioxidant Properties of Isolated Isorhamnetin from the Sea Buckthorn Marc

    Microsoft Academic Search

    Liu Pengfei; Deng Tiansheng; Hou Xianglin; Wang Jianguo

    2009-01-01

    In the present study, the process of separation and purification of isohamnetin from marc of sea buckthorn was obtained. The\\u000a antioxidant properties of the pure isolated isorhamnetin were evaluated by the scavenging of the diphenylpicrylhydrazyl radical\\u000a (DPPH), iron (III) to iron (II)—reducing, and iron-chelating assays. High purity isorhamnetin (92.1%) was obtained and the\\u000a results of antioxidant assays showed that isorhamnetin

  11. Antioxidant properties of sterilized yacon (Smallanthus sonchifolius) tuber flour.

    PubMed

    Sousa, Sérgio; Pinto, Jorge; Rodrigues, César; Gião, Maria; Pereira, Claúdia; Tavaria, Freni; Xavier Malcata, F; Gomes, Ana; Bertoldo Pacheco, M T; Pintado, Manuela

    2015-12-01

    The objective of this research work was to investigate the antioxidant properties of sterilized yacon tuber flour. The results revealed for the first time the high antioxidant activity of sterilized yacon flour. The best extract obtained by boiling 8.9% (w/v) of yacon flour in deionised water for 10min exhibited a total antioxidant capacity of 222±2mg (ascorbic acid equivalent)/100g DW and a total polyphenol content of 275±3mg (gallic acid equivalent)/100g DW associated to the presence of four main phenolic compounds: chlorogenic acid, caffeic acid, coumaric acid and protocatechuic acid, as well as the amino acid tryptophan. The most abundant was chlorogenic acid, followed by caffeic acid. Biological assays revealed that the extract had indeed antioxidant protection, and no pro-oxidant activity. In conclusion, sterilized yacon tuber flour has the potential to be used in the food industry as a food ingredient to produce functional food products. PMID:26041224

  12. Characterization of antioxidant methylcellulose film incorporated with ?-tocopherol nanocapsules.

    PubMed

    Noronha, Carolina Montanheiro; de Carvalho, Sabrina Matos; Lino, Renata Calegari; Barreto, Pedro Luiz Manique

    2014-09-15

    Biodegradable and antioxidant films based on methylcellulose (MC) and ?-tocopherol nanocapsule suspension (NCs) were developed. MC and NCs films were prepared by a casting method in three different proportions. The mechanical, wettability, colour, light transmission, antioxidant and release characteristics of the films were studied. The addition of NCs to MC films decreased the tensile strength (TS) and the elastic modulus (EM) (p<0.05) but increased the percentage elongation at break (%E) and thickness (p<0.05). NCs films showed a higher hydrophobicity when compared to that of film control. Lightness and yellowish color were intensified in the NCs films which, in their turn, demonstrated high antioxidant activity and excellent barrier properties against UV and visible light. A burst and prolonged release of ?-tocopherol to food simulant was also reported. PMID:24767092

  13. Characterization of an antioxidant polylactic acid (PLA) film prepared with ?-tocopherol, BHT and polyethylene glycol using film cast extruder

    Microsoft Academic Search

    Youngjae Byun; Young Teck Kim; Scott Whiteside

    2010-01-01

    Polylactic acid (PLA) films were prepared with ?-tocopherol, buthylated hydroxytoluene (BHT), and polyethylene glycol 400 (PEG 400) using a cast film extruder in an effort to create an antioxidant film. Film properties were characterized by radical scavenging activity and thermal, physical, and gas barrier properties. Final resin compounding and pelletization was achieved by a twin screw extruder and a pelletizator

  14. Effects of Genotype and Environment on the Antioxidant Properties of Hard Winter Wheat Bran

    E-print Network

    Liu, Jian-Guo

    Effects of Genotype and Environment on the Antioxidant Properties of Hard Winter Wheat Bran JEFFREY and dietary commodity worldwide with known antioxidant properties concentrated mostly in the bran fraction (E) to hard winter wheat bran antioxidant properties, as well as correlations of these properties

  15. Ferulic Acid: Therapeutic Potential Through Its Antioxidant Property

    PubMed Central

    Srinivasan, Marimuthu; Sudheer, Adluri R.; Menon, Venugopal P.

    2007-01-01

    There has been considerable public and scientific interest in the use of phytochemicals derived from dietary components to combat human diseases. They are naturally occurring substances found in plants. Ferulic acid (FA) is a phytochemical commonly found in fruits and vegetables such as tomatoes, sweet corn and rice bran. It arises from metabolism of phenylalanine and tyrosine by Shikimate pathway in plants. It exhibits a wide range of therapeutic effects against various diseases like cancer, diabetes, cardiovascular and neurodegenerative. A wide spectrum of beneficial activity for human health has been advocated for this phenolic compound, at least in part, because of its strong antioxidant activity. FA, a phenolic compound is a strong membrane antioxidant and known to positively affect human health. FA is an effective scavenger of free radicals and it has been approved in certain countries as food additive to prevent lipid peroxidation. It effectively scavenges superoxide anion radical and inhibits the lipid peroxidation. It possesses antioxidant property by virtue of its phenolic hydroxyl group in its structure. The hydroxy and phenoxy groups of FA donate electrons to quench the free radicals. The phenolic radical in turn forms a quinone methide intermediate, which is excreted via the bile. The past few decades have been devoted to intense research on antioxidant property of FA. So, the present review deals with the mechanism of antioxidant property of FA and its possible role in therapeutic usage against various diseases. PMID:18188410

  16. Antioxidant properties of a North American ginseng extract

    Microsoft Academic Search

    David D. Kitts; Arosha N. Wijewickreme; Chun Hu

    2000-01-01

    A North American ginseng extract (NAGE) containing known principle ginsenosides for Panax quinquefolius was assayed for metal chelation, affinity to scavenge DPPH-stable free radical, and peroxyl (LOO·) and hydroxyl (·OH) free radicals for the purpose of characterizing mechanisms of antioxidant activity. Dissociation constants (Kd) for NAGE to bind transition metals were in the order of Fe2+ > Cu2+ > Fe3+

  17. Antioxidant Properties of Whole Body Periodic Acceleration (pGz)

    PubMed Central

    Kurlansky, Paul; Altamirano, Francisco; Lopez, Jose R.

    2015-01-01

    The recognition that oxidative stress is a major component of several chronic diseases has engendered numerous trials of antioxidant therapies with minimal or no direct benefits. Nanomolar quantities of nitric oxide released into the circulation by pharmacologic stimulation of eNOS have antioxidant properties but physiologic stimulation as through increased pulsatile shear stress of the endothelium has not been assessed. The present study utilized a non-invasive technology, periodic acceleration (pGz) that increases pulsatile shear stress such that upregulation of cardiac eNOS occurs, We assessed its efficacy in normal mice and mouse models with high levels of oxidative stress, e.g. Diabetes type 1 and mdx (Duchene Muscular Dystrophy). pGz increased protein expression and upregulated eNOS in hearts. Application of pGz was associated with significantly increased expression of endogenous antioxidants (Glutathioneperoxidase-1(GPX-1), Catalase (CAT), Superoxide, Superoxide Dismutase 1(SOD1). This led to an increase of total cardiac antioxidant capacity along with an increase in the antioxidant response element transcription factor Nrf2 translocation to the nucleus. pGz decreased reactive oxygen species in both mice models of oxidative stress. Thus, pGz is a novel non-pharmacologic method to harness endogenous antioxidant capacity. PMID:26133377

  18. Synthesis, characterization, antioxidative activity and DNA binding properties of the copper(II), zinc(II), nickel(II) complexes with 1,2-Di(4'-iminonaringenin)ethane.

    PubMed

    Li, Yong; Yang, Zheng-Yin; Li, Tian-Rong

    2008-11-01

    A novel naringenin Schiff base ligand (1,2-di(4'-iminonaringenin)ethane, H6L) and its three transition metal complexes [Cu(II) complex (1), Zn(II) complex (2), and Ni(II) complex (3)] have been prepared and characterized on the basis of elemental analysis, molar conductivity, 1H-NMR, mass spectra, UV-vis spectra, and IR spectra. The DNA-binding properties of the ligand and its complexes have been investigated by absorption spectroscopy, fluorescence spectroscopy, ethidium bromide (EB) displacement experiments, and viscosity measurement. The results indicated that the ligand and its complexes can bind to DNA. The binding affinity of the Cu(II) complex (1) is higher than that of the ligand and the other two complexes. The intrinsic binding constant (Kb) of the complex (1) is 3.3x10(6). In addition, the suppression ratio for O2-. and HO. was determined. The 50% inhibition obtained for the ligand and its three complexes demonstrates that, compared to the ligand, the complexes exhibit higher antioxidative activity in the suppression of O2-. and HO.. PMID:18981601

  19. Antioxidant Properties of Aminoethylcysteine Ketimine Decarboxylated Dimer: A Review

    PubMed Central

    Macone, Alberto; Fontana, Mario; Barba, Marco; Botta, Bruno; Nardini, Mirella; Ghirga, Francesca; Calcaterra, Andrea; Pecci, Laura; Matarese, Rosa Marina

    2011-01-01

    Aminoethylcysteine ketimine decarboxylated dimer is a natural sulfur-containing compound detected in human plasma and urine, in mammalian brain and in many common edible vegetables. Over the past decade many studies have been undertaken to identify its metabolic role. Attention has been focused on its antioxidant properties and on its reactivity against oxygen and nitrogen reactive species. These properties have been studied in different model systems starting from plasma lipoproteins to specific cellular lines. All these studies report that aminoethylcysteine ketimine decarboxylated dimer is able to interact both with reactive oxygen and nitrogen species (hydrogen peroxide, superoxide anion, hydroxyl radical, peroxynitrite and its derivatives). Its antioxidant activity is similar to that of Vitamin E while higher than other hydrophilic antioxidants, such as trolox and N-acetylcysteine. PMID:21686170

  20. Antioxidant Properties of Tea Extracts as Affected by Processing

    Microsoft Academic Search

    L Manzocco; M Anese; M. C Nicoli

    1998-01-01

    The changes in the antioxidant properties of green and black tea extracts as a consequence of processing and storage conditions were evaluated through measurements of chain-breaking activity, oxygen scavenging capacity and redox potential. Pasteurisation and storage as well as forced oxygenation in both tea extracts caused an increase in the optical density and a decrease in the reducing power of

  1. Effects of Solid-State Yeast Treatment on the Antioxidant Properties and Protein and Fiber

    E-print Network

    Liu, Jian-Guo

    Effects of Solid-State Yeast Treatment on the Antioxidant Properties and Protein and Fiber, including extractable antioxidant properties, protein contents, and soluble and insoluble fiber compositions treatments were able to significantly increase releasable antioxidant properties ranging from 28 to 65, from

  2. A density functional study of antioxidant properties on anthocyanidins

    NASA Astrophysics Data System (ADS)

    Guzmán, Rosa; Santiago, Cristobalina; Sánchez, Mario

    2009-10-01

    A density functional theory (DFT) study, using the B3LYP/6-31G(d,p) method, was performed in a attempt to understand the antioxidant properties of some anthocyanidins. This study is based on the H-atom transfer mechanism, which implicates the evaluation of the bond dissociation enthalphy (BDE) of all OH substituents in each structure. The electronic structures studied in this paper are: aurantinidin, cyanidin, delphinidin, malvinidin, pelargonidin and peonidin. Analysis of the computed results suggest that the antioxidant capacity of those structures is in the following order: cyanidin > malvidin > aurantinidin > delphinidin ? peonidin > pelargonidin.

  3. Chemical and antioxidant properties of pomegranate cultivars grown in the Mediterranean region of Turkey

    Microsoft Academic Search

    Mustafa Ozgen; Co?kun Durgaç; Sedat Serçe; Cemal Kaya

    2008-01-01

    Arils from six pomegranate (Punica granatum L.) cultivars obtained from various sites from the Mediterranean region of Turkey were evaluated for their chemical and antioxidant properties. These properties included total phenolics (TP), total monomeric anthocyanins (TMA), soluble solids (TSS), titratable acidity (TA), individual sugars and organic acids. Antioxidant capacities of arils were determined by both the ferric reducing antioxidant power

  4. Profiling and characterization antioxidant activities in Anoectochilus formosanus hayata.

    PubMed

    Wang, Sheng-Yang; Kuo, Yueh-Hsiung; Chang, Hsing-Ning; Kang, Pei-Ling; Tsay, Hsin-Sheng; Lin, Ku-Feng; Yang, Ning-Sun; Shyur, Lie-Fen

    2002-03-27

    Phytochemical characteristics and antioxidant activities of the crude and fractionated plant extracts of Anoectochilus formosanus were evaluated using five different assay systems. An acid-treatment (2 N HCl in 95% ethanol) was employed to treat a butanol fraction (BuOH), creating an acid-hydrolyzed BuOH fraction. The IC(50) values for DPPH radicals in the BuOH and acid-hydrolyzed BuOH fractions were 0.521 and 0.021 mg/mL, respectively. The acid-hydrolyzed BuOH exhibited approximately 5-fold higher activity in scavenging superoxide anion than catechin. The acid-hydrolyzed BuOH fraction also effectively protected phi x174 supercoiled DNA against strand cleavage induced by H(2)O(2) and reduced oxidative stress in HL-60 cells. Metabolite profiling showed that the aglycones of flavonoid glycosides in BuOH were produced after acid hydrolytic treatment, and this resulted in a significant increase in antioxidant activities of acid-hydrolyzed BuOH. One new diarylpentanoid, kinsenone, and three known flavonoid glycosides and their derivatives were identified for the first time from A. formosanus, with strong antioxidant properties. PMID:11902924

  5. Antioxidant and antimicrobial properties of monofloral bee pollen.

    PubMed

    Fatrcová-Šramková, Katarína; Nôžková, Janka; Ka?ániová, Miroslava; Máriássyová, Magda; Rovná, Katarína; Stri?ík, Michal

    2013-01-01

    The main aim of this study was to determine antioxidant properties and antibacterial activity of monofloral bee pollen samples to pathogenic bacteria. These samples were collected in different localities in Slovakia. The antioxidant properties of examined plant species were different and decreasing in the following order: Brassica napus subsp. napus L > Papaver somniferum L. > Helianthus annuus L. The antimicrobial effect of the bee product samples were tested by using the agar well diffusion method. The methanol (99.9% and 70%) and the ethanol (96% and 70%) were used for extraction. In this study, five different strains of bacteria were tested: Listeria monocytogenes CCM 4699; Pseudomonas aeruginosa CCM 1960; Staphylococcus aureus CCM 3953; Salmonella enterica CCM 4420; and Escherichia coli CCM 3988. The most sensitive bacteria of the poppy pollen ethanolic extract was Staphylococcus aureus was (70%) The most sensitive bacteria of rape bee pollen methanolic extract (70%) and sunflower ethanolic extract (70%) was Salmonella enterica. PMID:23305281

  6. Resveratrol content and antioxidant properties of underutilized fruits.

    PubMed

    Shrikanta, Akshatha; Kumar, Anbarasu; Govindaswamy, Vijayalakshmi

    2015-01-01

    In the present study, resveratrol content and antioxidant properties of underutilized fruits such as Jamun (Syzygium cumini L.), Jackfruit (Artocarpus heterophyllus) and Mulberry (Morus rubra) were investigated keeping Grape (Vitis vinifera) as a reference. Ethanol/water (80:20 v/v) extracts of different parts of fruit samples including skin, pulp and seeds were analyzed by HPLC and MS for the quantification of resveratrol. Total polyphenols, flavonoids, DPPH scavenging activity and total antioxidant capacity were also investigated. Among the samples analyzed, mulberry fruit (whole) showed highest resveratrol content (50.61 ?g g(-1) dry weight) followed by jamun seed (34.87 ?g g(-1) dry weight), jamun pulp (13.70 ?g g(-1) dry weight) and skin of jamun (11.19 ?g g(-1) dry weight). Jamun seed extract exhibited the highest polyphenol content (55.54 mg gallic acid equivalent g(-1) dry weight) and highest antioxidant property (IC50 value-0.40 mg ml(-1)). The results suggest that underutilized fruits high in resveratrol and other polyphenols can be used as functional beverages. PMID:25593373

  7. Regulation of biosynthetic genes and antioxidant properties of vitamin B6 vitamers during plant defense responses

    E-print Network

    Daub, Margaret

    Regulation of biosynthetic genes and antioxidant properties of vitamin B6 vitamers during plant linked to cellular oxidative stress. We investigated the role of this vitamin as an antioxidant in oxidative responses linked to plant defense. B6 vitamers effectively quenched superoxide and had antioxidant

  8. ANTIOXIDANT PROPERTIES OF ENDOXAN IN RENAL MODEL SYSTEMS Milka Mileva1

    E-print Network

    Boyer, Edmond

    1 ANTIOXIDANT PROPERTIES OF ENDOXAN IN RENAL MODEL SYSTEMS Milka Mileva1 , Lubomir Traikov1 and a decrease in the level of non-enzymatic antioxidants, such as reduced glutathione and vitamins C, E and A, as well as total antioxidant status. According some authors endoxan is an inactive cytostatic which

  9. Antioxidant and sensorial properties of linden honey with dried apricots.

    PubMed

    ?etkovi?, Gordana; ?anadanovi?-Brunet, Jasna; Vuli?, Jelena; Djilas, Sonja; Tumbas Šaponjac, Vesna

    2014-11-01

    The total phenol (TPh) and flavonoid contents (TFd), and antioxidant and sensorial properties of linden honey (LH) with dried apricots (20, 30, and 40%) were evaluated. TPh increased 4.3 times for LH40 (from 23.96 to 102.87?mg gallic acid equiv./100?g honey), while increase of TFd was slightly lower, ca. 2.9-fold for LH40 (from 18.11 to 51.72?mg rutin equiv./100?g honey). Based on HPLC analysis, the most dominant phenolic compound was gallic acid (11.14?mg/100?g honey in LH and 42.65?mg/100?g honey in LH40). In three different assays, the antioxidant activity increased with increasing concentration of apricots in honey. The values varied from 13.36 for LH to 7.06?mg/ml for LH40; the values ranged from 189.83 for LH to 11.23?mg/ml for LH40; the RP0.5 (reducing power) values ranged from 169.00 for LH to 27.60?mg/ml for LH40. Based on the correlation analysis, it is obvious that TPh and TFd were associated with the antioxidant activities of honey samples. A high degree of correlation existed between antioxidant activities of honey samples and TPh (R from 0.945 to 0.996) and TFd (R from 0.805 to 0.934). Obtained scores for individual sensory properties indicated very good quality of honey with dried apricots. PMID:25408327

  10. Antioxidants

    MedlinePLUS

    ... doses of vitamin E may increase risks of prostate cancer and one type of stroke. Antioxidant supplements may also interact with some medicines. To minimize risk, tell you of your health care providers about any antioxidants you use. NIH: National Center for Complementary and Integrative Health

  11. [Structure elucidation of antioxidative polyphenols and their biological properties].

    PubMed

    Yoshimura, Morio

    2014-01-01

    Tannins and related polyphenols have been reported to produce diverse biological effects that indicate their therapeutic potential, including antioxidative, antibacterial, and antiviral activities. In an exploratory study of natural antioxidants with biological effects, we investigated the tannin constituents of myrtaceous plants reported to be rich in polyphenols. We isolated 16 new compounds from five species of the Melaleuca, Eucalyptus, Syzygium, Pimenta, and Myrtus genera. Among the new polyphenols, nine were isolated from Melaleuca squarrosa and characterized as C-glucosidic ellagitannin monomers and oligomers. The structures of the other new polyphenols from myrtaceous plants and a novel compound obtained from the berries of Pyracantha coccinea (Rosaceae) were also elucidated based on spectroscopic and chemical evidence. The antioxidative activity of isolates in the present study was estimated by measuring 1,1-diphenyl-2-picrylhydrazyl radical (DPPH)-scavenging activity and in the oxygen radical absorbance capacity (ORAC) test. We found that oenothein B, a macrocyclic ellagitannin dimer, is widely distributed in the genus Eucalyptus and produces a significant immunomodulatory effect on human dendritic cells (DCs). In a survey of natural ligands that bind to the aryl hydrocarbon receptor (AhR), nepitrin, a flavonoid glycoside, and other polyphenols were revealed to be promising therapeutic candidates. PMID:25174366

  12. Spirogyra varians mutant generated by high dose gamma-irradiation shows increased antioxidant properties

    NASA Astrophysics Data System (ADS)

    Lee, Hak-Jyung; Yoon, Minchul; Sung, Nak-Yun; Choi, Jong-il

    2012-08-01

    The aim of this study was to evaluate the antioxidant properties of a Spirogyra varians mutant (Mut) produced by gamma irradiation. Methanol extracts were prepared from Spirogyra varians wild-type and Mut plants, and their antioxidant activities and total phenolic content (TPC) were determined. Antioxidant parameters, including the 2-diphenyl-1-picrylhydrazyl radical-scavenging activity and ferric-reducing/antioxidant power, were higher in the Mut extract. Moreover, the TPC level was higher (P<0.05) in the Mut methanol extract. Therefore, these results suggest that gamma irradiation-induced S. varians Mut has superior antioxidant properties.

  13. Chemical Characterization, Bioactive Properties, and Pigment Stability of Polyphenolics in Açai (Euterpe oleracea Mart.)

    E-print Network

    Pacheco Palencia, Lisbeth A.

    2010-07-14

    Phytochemical composition, antioxidant activity, pigment stability, bioactive properties, and in-vitro absorption of polyphenolics in acai fruit (Euterpe oleracea Mart.) were investigated. Detailed characterization of phenolic compounds present...

  14. Antimutagenic, antioxidant and antimicrobial properties of Maytenus krukovii bark.

    PubMed

    Bruni, Renato; Rossi, Damiano; Muzzoli, Mariavittoria; Romagnoli, Carlo; Paganetto, Guglielmo; Besco, Elena; Choquecillo, Fritz; Peralta, Katia; Lora, William Schmitt; Sacchetti, Gianni

    2006-12-01

    The hydroalcoholic extract of Maytenus krukovii bark was investigated for its in vitro mutageno-protective activities by means of the Ames Salmonella/microsome assay. The extract showed an inhibitory effect in both T98 and T100 strains against the mutagenic activity of promutagen 2-aminoanthracene but was not protective against directly acting mutagens sodium azide and 2-nitrofluorene. When tested as a radical scavenger and antioxidant it produced a dose-dependent inhibition. The extract did not show significant antibacterial properties, and was weakly active against dermatophyte and phytopathogenic fungi, but inhibited the growth of phytopathogen Pithyum ultimum. PMID:16963198

  15. Identification and characterization of antioxidant peptides from chickpea protein hydrolysates.

    PubMed

    Torres-Fuentes, Cristina; Contreras, María del Mar; Recio, Isidra; Alaiz, Manuel; Vioque, Javier

    2015-08-01

    Oxidative stress due to the excess of radical oxygen species (ROS) contribute to the development of different diseases. The use of antioxidants may prevent the development of these diseases by counteracting ROS levels. There is an increasing interest in natural antioxidants as they are safer for consumers than synthetic antioxidants. In this work, reducing power, free radical scavenging and cellular antioxidant activities of chickpea peptides fractions have been investigated. Peptide sequences included in fractions with antioxidant activity were identified. Main sequences, ALEPDHR, TETWNPNHPEL, FVPH and SAEHGSLH, corresponded to legumin, the main seed protein. Most peptides contained histidine, which has shown antioxidant activity. Two peptides also included tryptophan and phenylalanine, in which the phenolic group could also serve as hydrogen donor. These results show that legumin is a source of antioxidant peptides of high interest for food and pharmaceutical industries to develop new nutraceuticals and functional foods. PMID:25766818

  16. Assessment of oxidative stress and antioxidant property using electron spin resonance (ESR) spectroscopy.

    PubMed

    Lee, Masaichi-Chang-Il

    2013-01-01

    The pathophysiology of hypertension or stroke is associated with an excess of ROS generation in the vascular system, and results in induction of various pathological cascades of cerebrovascular damage. We have demonstrated that electron spin resonance methods using a spin trap or spin probe will be useful for understanding redox status under conditions of oxidative stress in the spontaneously hypertensive rat or stroke-prone spontaneously hypertensive rat brain. We have used electron spin resonance imaging and noninvasive L-band electron spin resonance to characterize the higher degree of brain oxidative stress in the stroke-prone spontaneously hypertensive rat and spontaneously hypertensive rat than in the Wistar-Kyoto rat brain, and the lower extent of oxidative stress in the spontaneously hypertensive rat than in the stroke-prone spontaneously hypertensive rat brain. Indeed, we may be able to confirm propofol medium-chain triglyceride/long-chain triglyceride (MCT/LCT) as neuroprotective anesthesia and crocetin as antioxidant food factor against human stroke after screening for antioxidant properties in stroke models such as stroke-prone spontaneously hypertensive rat. Thus, our electron spin resonance biomedical application suggests that it could be used to assess antioxidant effects on oxidative stress in the brain using spontaneously hypertensive rat and stroke-prone spontaneously hypertensive rat. We hope that further advances in the instrumentation used for electron spin resonance imaging and the development of optimized nontoxic spin probes will make this technology even more promising for novel clinical prediction or noninvasive diagnosis of human stroke. After screening drugs or foods for antioxidant property using in vitro or in vivo electron spin resonance assessment, it will be possible to find and develop novel drugs or food factors with such properties for the prevention of stroke in the near future. PMID:23341690

  17. Scavenging and antioxidant properties of compounds synthesized by carotenogenic yeasts stressed by heavy metals--EPR spin trapping study.

    PubMed

    Rapta, Peter; Polovka, Martin; Zalibera, Michal; Breierova, Emilia; Zitnanova, Ingrid; Marova, Ivana; Certik, Milan

    2005-06-01

    Free radical scavenging and antioxidant activities of metabolites produced by carotenogenic yeasts of Rhodotorula sp. and Sporobolomyces sp. grown under heavy metal presence were studied using various EPR experiments. The thermally initiated decomposition of K(2)S(2)O(8) coupled with EPR spin trapping was shown to be the best choice to characterize antioxidant properties of yeast's samples. EPR spectroscopy revealed that yeast walls showed higher ability to scavenge free radicals than those from inside the cells. Since carotenogenic yeast differ to each other in resistance against the heavy metals due to their individual protective system, quenching properties and antioxidant activities of carotenogenic yeasts were modulated by Ni(2+) or Zn(2+) ions variously. PMID:15911077

  18. Characterization of antioxidant-antimicrobial ?-carrageenan films containing Satureja hortensis essential oil.

    PubMed

    Shojaee-Aliabadi, Saeedeh; Hosseini, Hedayat; Mohammadifar, Mohammad Amin; Mohammadi, Abdorreza; Ghasemlou, Mehran; Ojagh, Seyed Mahdi; Hosseini, Seyede Marzieh; Khaksar, Ramin

    2013-01-01

    The present work was aimed at characterizing biodegradable composite kappa-carrageenan films incorporated with Satureja hortensis (SEO) in terms of their physical, optical, mechanical, barrier and antioxidant properties. Also, in a comparative study, we sought to evaluate the antimicrobial effectiveness of these films against five pathogens. The films' water vapor barrier properties were found to improve considerably upon the addition of SEO. Carrageenan composite films were less resistant to breakage, more flexible and more opaque with lower gloss than the control film. These results can be explained by the film's microstructure, which was analyzed by atomic force microscopy and scanning electron microscopy. The films incorporating SEO showed good antioxidant properties; this effect was greatly improved when the proportion of added SEO was 3%. Films with SEO effectively inhibited the five microorganisms tested. The results of the present study suggest that SEO as a natural antibacterial agent can potentially be used in packaging a wide range of food products, particularly those that are highly oxidative and microbial sensitive. PMID:22959956

  19. Chemistry and pharmacological properties of some natural and synthetic antioxidants for heavy metal toxicity.

    PubMed

    Flora, S J S; Shrivastava, Rupal; Mittal, Megha

    2013-01-01

    Heavy metals are known to cause oxidative deterioration of bio-molecules by initiating free radical mediated chain reaction resulting in lipid per-oxidation, protein oxidation and oxidation of nucleic acid like DNA and RNA. The development of effective dual functioning antioxidants, possessing both metal-chelating and free radical-scavenging properties should bring into play. Administration of natural and synthetic antioxidants like, quercetin, catechin, taurine, captopril, gallic acid, melatonin, N-acetyl cysteine, ?- lipoic acid and others have been recognized in the disease prevention and clinical recovery against heavy metal intoxication. These antioxidants affect biological systems not only through direct quenching of free radicals but also via chelation of toxic metal(s). These antioxidants also, have the capacity to enhance cellular antioxidant defense mechanism by regenerating endogenous antioxidants, such as glutathione and vitamin C and E. They also influence cellular signaling and trigger redox sensitive regulatory pathways. The reactivity of antioxidants in protecting against heavy metal induced oxidative stress depends upon their structural properties, their partitioning abilities between hydrophilic and lipophilic environment and their hydrogen donation antioxidant properties. Herein, we review the structural, biochemical and pharmacological properties of selected antioxidants with particular reference to their ability to (i) chelate heavy metals from its complex (ii) ameliorate free radical (iii) terminate heavy metal induced free radical chain reaction (iv) regenerate endogenous antioxidants and, (v) excretion of metal without its redistribution. PMID:24206124

  20. Antioxidant Characterization of Oak Extracts Combining Spectrophotometric Assays and Chemometrics

    PubMed Central

    Popovi?, Boris M.; Štajner, Dubravka; Orlovi?, Saša; Gali?, Zoran

    2013-01-01

    Antioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against DPPH•, •NO, and O2•? radicals, ferric reducing antioxidant power (FRAP), total phenol, tannin, flavonoid, and proanthocyanidin contents. Permanganate reducing antioxidant capacity (PRAC) was determined using water extracts. Beside, mentioned parameters, soluble proteins, lipid peroxidation (LP), pigments and proline contents were also determined. The data of different procedures were compared and analyzed by multivariate techniques (correlation matrix calculation and principal component analysis (PCA)). PCA found that investigated organs of two different oak tree species possess similar antioxidant characteristics. The superior antioxidant characteristics showed oak leaves over twigs and acorns and seem to be promising source of antioxidants with possible use in industry and pharmacy. PMID:24453789

  1. Anti-inflammatory and antioxidant properties of Helichrysum italicum.

    PubMed

    Sala, Araceli; Recio, MaríadelCarmen; Giner, Rosa Marfa; Máñez, Salvador; Tournier, Horacio; Schinella, Guillermo; Ríos, José-Luis

    2002-03-01

    The anti-inflammatory and antioxidant activities of the aerial part of Helichrysum italicum extracts have been established in various in-vivo and in-vitro experimental models. The results obtained on the acute oedemas induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) and ethyl phenylpropiolate in the mouse ear, by serotonin and phospholipase A2 (PLA2) in the mouse paw, on chronic inflammation induced by repeated application of TPA in the mouse ear and on the delayed-type hypersensitivity induced by sheep red blood cells suggest that said anti-inflammatory activity is due to the effects of compounds expressed via a corticoid-like mechanism. In addition, the antioxidant activity of the extracts seems to be implicated in this anti-inflammatory activity, as the former inhibits enzymatic and non-enzymatic lipid peroxidation and has free-radical scavenger properties. We conclude that the anti-inflammatory activity of Helichrysum italicum can be explained by multiple effects, including inflammatory enzyme inhibition, free-radical scavenging activity and corticoid-like effects. PMID:11902802

  2. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus

    PubMed Central

    Mu, Haibo; Zhang, Amin; Zhang, Wuxia; Cui, Guoting; Wang, Shunchun; Duan, Jinyou

    2012-01-01

    The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties against chemicals-induced reactive species (ROS) including 1,1?-Diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide anion radical, as well as their protective effects on H2O2-induced PC12 cell death were investigated. Results showed that I. obliquus polysaccharides can scavenge all ROS tested above in a dose-dependent manner. IOA and its product IOA-1 could rescue PC12 cell viability from 38.6% to 79.8% and 83.0% at a concentration of 20?g/mL. Similarly, IOW and its product IOW-1 at the same dose, can also increase cell viability to 84.9% and 88.6% respectively. The antioxidative activities of water-soluble and alkali-soluble polysaccharide constituents from I. obliquus might contribute to diverse medicinal and nutritional values of this mushroom. PMID:22942760

  3. Antioxidant properties of domesticated and wildRubus species

    Microsoft Academic Search

    Nigel Deighton; Rex Brennan; Chad Finn; Howard V Davies

    2000-01-01

    The antioxidative capacities of a number of Rubus species of varied pigmentation have been investigated. In addition, total phenol, anthocyanin and ascorbic acid contents have been determined. Two methods to assess the antioxidant potential of fruit juices have been used. The antioxidant capacities of the fruit ranged from 0 to 25.3 mmol Trolox equivalents g?1 (TEAC) or from 190 to

  4. The chemistry and antioxidant properties of tocopherols and tocotrienols

    Microsoft Academic Search

    Afaf Kamal-Eldin; Lars-Åke Appelqvist

    1996-01-01

    This article is a review of the fundamental chemistry of the tocopherols and tocotrienols relevant to their antioxidant action.\\u000a Despite the general agreement that ?-tocopherol is the most efficient antioxidant and vitamin E homologuein vivo, there was always a considerable discrepancy in its “absolute” and “relative” antioxidant effectivenessin vitro, especially when compared to ?-tocopherol. Many chemical, physical, biochemical, physicochemical, and

  5. Antioxidant properties of black tea in alcohol intoxication

    Microsoft Academic Search

    W. ?uczaj; E. Skrzydlewska

    2004-01-01

    Food ingredients such as alcohol may modify cellular redox state. Ethanol metabolism is accompanied by generation of free radicals that can damage cell components especially when antioxidant mechanisms are no able to neutralize them. However black tea is a source of polyphenol antioxidants that may enhance cellular antioxidant abilities. The aim of this study was to investigate the effect of

  6. Quercetin and rutin\\/2-hydroxypropyl-?-cyclodextrin nanoparticles: obtaining, characterization and antioxidant activity

    Microsoft Academic Search

    Georgeta Coneaca; Nicoleta G. H?d?rug?c; Daniel I. H?d?rug?d

    This paper presents the obtaining, characterization and antioxidant activity evaluation of quercetin\\/2- hydroxypropyl-?-cyclodextrin and rutin\\/2-hydroxypropyl-?-cyclodextrin complexes. For synthesis the crystallization from ethanol-water solution method was used, but the yields were relatively low (up to 23%). The morphology and dimensional characterization was realized by using scanning electron microscopy. The formation of complexes was revealed by differential scanning calorimetry and the antioxidant

  7. Hydroalcoholic extracts of Indian medicinal plants can help in amelioration from oxidative stress through antioxidant properties.

    PubMed

    Sarkar, Rhitajit; Mandal, Nripendranath

    2012-01-01

    The in vitro study of the antioxidant properties of the hydroalcoholic extracts of various Indian medicinal plants can logically help to develop a better and safer way of amelioration from oxidative stress. As aimed, the present study has been done to estimate and thereby conclude regarding the antioxidant activities of a few Indian medicinal plants, viz., Terminalia chebula, Terminalia belerica, Emblica officinalis, Caesalpinia crista, Cajanus cajan, and Tinospora cordifolia. The extracts of the plants have been subjected to the evaluation of antioxidant properties through scavenging assays for reactive oxygen species like superoxide, nitric oxide, peroxynitrite, hypochlorous acid, singlet oxygen, etc. and measurement of TEAC values and other phytochemical parameters. The phenolic and flavonoid contents of each plant have been found to be correlated to their individual antioxidant activity. The results showed the hydroalcoholic extracts of the plants were efficient indicators of their antioxidant capacity thus concreting their basis to be used as natural antioxidant. PMID:22624183

  8. Microwave-assisted synthesis and antioxidant properties of hydrazinyl thiazolyl coumarin derivatives

    PubMed Central

    2012-01-01

    Background Coumarin derivatives exhibit a wide range of biological properties including promising antioxidant activity. Furthermore, microwave-assisted organic synthesis has delivered rapid routes to N- and O-containing heterocycles, including coumarins and thiazoles. Combining these features, the use of microwave-assisted processes will provide rapid access to a targeted coumarin library bearing a hydrazino pharmacophore for evaluation of antioxidant properties Results Microwave irradiation promoted 3 of the 4 steps in a rapid, convergent synthesis of a small library of hydrazinyl thiazolyl coumarin derivatives, all of which exhibited significant antioxidant activity comparable to that of the natural antioxidant quercetin, as established by DPPH and ABTS radical assays Conclusions Microwave dielectric heating provides a rapid and expedient route to a series of hydrazinyl thiazolyl coumarins to investigate their radical scavenging properties. Given their favourable properties, in comparison with known antioxidants, these coumarin derivatives are promising leads for further development and optimization. PMID:22510146

  9. Standardization of antioxidant properties of honey by a combination of spectrophotometric\\/fluorimetric assays and chemometrics

    Microsoft Academic Search

    Giangiacomo Beretta; Paola Granata; Maria Ferrero; Marica Orioli; Roberto Maffei Facino

    2005-01-01

    The aim of this work was to establish a solid platform of analytical information for the definition\\/standardization of the antioxidant properties of honey. We investigated first the antioxidant\\/radical scavenging capacity of 14 commercial honeys of different floral and geographic origin, using a battery of spectrophotometric tests: Folin-Ciocalteu assay for phenol content (PC), ferric reducing antioxidant power (FRAP assay) for total

  10. Antioxidant Properties of the Edible Basidiomycete Armillaria mellea in Submerged Cultures

    PubMed Central

    Lung, Ming-Yeou; Chang, Yu-Cheng

    2011-01-01

    Antioxidant components, ascorbic acid, total flavonoids and total phenols are produced effectively by Armillaria mellea submerged cultures. Dried mycelia and mycelia-free broths obtained by A. mellea submerged cultures are extracted with methanol and hot water and investigated for antioxidant properties. Methanolic extracts from dried mycelia (MEM) and mycelia-free broth (MEB) and hot water extracts from dried mycelia (HWEM) by A. mellea submerged cultures show good antioxidant properties as evidenced by low EC50 values (<10 mg/mL). Total flavonoid is mainly found in hot water extracts; however, total phenol is rich in methanol and hot water extracts from mycelia. Ascorbic acid and total phenol contents are well correlated with the reducing power and the scavenging effect on superoxide anions. Total flavonoid content is dependent on the antioxidant activity and the chelating effect on ferrous ions. Total antioxidant component contents are closely related to the antioxidant activity and the scavenging superoxide anion ability. Results confirm that extracts with good antioxidant properties from fermenting products by A. mellea are potential good substitutes for synthetic antioxidants and can be applied to antioxidant-related functional food and pharmaceutical industries. PMID:22072892

  11. Antioxidative properties and flavonoid composition of Chenopodium quinoa seeds cultivated in Japan

    Microsoft Academic Search

    Yuko Hirose; Tomoyuki Fujita; Toshiyuki Ishii; Naoya Ueno

    2010-01-01

    To evaluate the nutritional advantages of quinoa seeds (Chenopodium quinoa Willd.) cultivated in Japan, antioxidative properties and flavonoid composition were determined and compared to corresponding data for conventionally-used cereals and pseudo-cereals, including quinoa seeds from South America. The antioxidant activities of these grains against DPPH radicals were strongly associated with the total phenolic content of the tested samples. The crude

  12. Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids

    Microsoft Academic Search

    Shay Yehoshua Schubert; Ephraim Philip Lansky; Ishak Neeman

    1999-01-01

    The antioxidant and eicosanoid enzyme inhibition properties of pomegranate (Punica granatum) fermented juice and seed oil flavonoids were studied. The pomegranate fermented juice (pfj) and cold pressed seed oil (pcpso) showed strong antioxidant activity close to that of butylated hydroxyanisole (BHA) and green tea (Thea sinensis), and significantly greater than that of red wine (Vitis vitifera). Flavonoids extracted from pcpso

  13. Chemical composition and the antioxidative properties of Nigerian Okra Seed ( Abelmoschus esculentus Moench) Flour

    Microsoft Academic Search

    O. E. Adelakun; O. J. Oyelade; B. I. O. Ade-Omowaye; I. A. Adeyemi; M. Van de Venter

    2009-01-01

    Studies on the chemical composition and the antioxidative properties of Nigerian Okra Seed (Abelmoschus esculentus Moench) Flour were carried out. This is done to establish the nutritional composition and the antioxidative potentials of the seeds, both of which are highly implicated in health. Okra seeds were roasted at 160°C for 10–60mins. The roasted seeds were subjected to proximate, yield and

  14. Redispersible liposomal-N-acetylcysteine powder for pulmonary administration: development, in vitro characterization and antioxidant activity.

    PubMed

    Ourique, Aline Ferreira; Chaves, Paula Dos Santos; Souto, Gabriele Dadalt; Pohlmann, Adriana Raffin; Guterres, Silvia Stanisçuaski; Beck, Ruy Carlos Ruver

    2014-12-18

    Liposomal dry powders of N-acetylcysteine (SD-NAC-Lip) were developed for pulmonary administration. Liposomes were prepared by reverse phase evaporation and spray dried using lactose (10%, w/w) as drying adjuvant. The powders were characterized according to process yield, drug content, residual water content, particle size distribution, morphology and redispersion behavior. In vitro aerosol performance was evaluated using an eight-stage Andersen Cascade Impactor. Moreover, in vitro antioxidant activity was determined by measuring thiobarbituric acid reactive species (TBARS) present in the lungs of healthy Wistar rats after induction of oxidation by iron/EDTA. The spray-drying process had a high yield (71%±2), drug content (mg/g) according to the expected value, moisture content below 9%, geometric mean diameter under 3?m with span value lower than 1. Spherical particles were observed by scanning electron microscopy. Liposomal dry-powders were able to recover the nanometric size of the original dispersion after their redispersion in aqueous medium, as shown by laser diffraction and transmission electron microscopy. Furthermore, the powders presented aerodynamic diameter of about 7?m and respirable fraction above 30%, indicating suitable properties for pulmonary use. The encapsulation of N-acetylcysteine in liposomes was essential to maintain its in vitro antioxidant activity after the drying process. In addition, the powder containing the encapsulated drug had better in vitro antioxidant activity than the liquid and solid formulations containing the non-encapsulated drug, which makes it a good candidate for the treatment of pulmonary diseases associated with oxidative stress. PMID:25263567

  15. The effect of heating and fermenting on antioxidant properties of white cabbage.

    PubMed

    Kusznierewicz, Barbara; ?miechowska, Anna; Bartoszek, Agnieszka; Namie?nik, Jacek

    2008-06-01

    It is widely believed that natural antioxidants found in food are significantly lost during processing. Nevertheless, it was recently demonstrated that processed fruits and vegetables may retain their antioxidant activity. In the present work, the changes in the overall antioxidant properties as a consequence of fermentation of cabbage and/or heat treatment of cabbage juices and extracts were studied. Fermentation processes as well as heat treatment increased the initial values of antioxidant activity. While a decrease in the antioxidant potential of sauerkraut juice was found for short heat treatments, a partial recovery of these properties was observed by prolonging heating periods. The TLC analysis showed that during fermentation and thermal processes, some substances with reactivity towards Folin-Ciocalteu reagent, hence with possible antioxidant activity, were released. We demonstrated that in contrast to common expectation, typical culinary processing of cabbage increases its antioxidant potency. The gain in antioxidant activity of heated samples coincided with the formation of both F-C reagent reactive compounds as well as brown early Maillard reaction products. This information may encourage the consumption of heat processed cabbage, especially that the release of antioxidants during heating may prevent oxidation of other food components, e.g. fats. PMID:26065745

  16. Antioxidative and Chemopreventive Properties of Vernonia amygdalina and Garcinia biflavonoid

    PubMed Central

    Farombi, Ebenezer O.; Owoeye, Olatunde

    2011-01-01

    Recently, considerable attention has been focused on dietary and medicinal phytochemicals that inhibit, reverse or retard diseases caused by oxidative and inflammatory processes. Vernonia amygdalina is a perennial herb belonging to the Asteraceae family. Extracts of the plant have been used in various folk medicines as remedies against helminthic, protozoal and bacterial infections with scientific support for these claims. Phytochemicals such as saponins and alkaloids, terpenes, steroids, coumarins, flavonoids, phenolic acids, lignans, xanthones, anthraquinones, edotides and sesquiterpenes have been extracted and isolated from Vernonia amygdalina. These compounds elicit various biological effects including cancer chemoprevention. Garcinia kola (Guttiferae) seed, known as “bitter kola”, plays an important role in African ethnomedicine and traditional hospitality. It is used locally to treat illnesses like colds, bronchitis, bacterial and viral infections and liver diseases. A number of useful phytochemicals have been isolated from the seed and the most prominent of them is the Garcinia bioflavonoids mixture called kolaviron. It has well-defined structure and an array of biological activities including antioxidant, antidiabetic, antigenotoxic and hepatoprotective properties. The chemopreventive properties of Vernonia amygdalina and Garcinia biflavonoids have been attributed to their abilities to scavenge free radicals, induce detoxification, inhibit stress response proteins and interfere with DNA binding activities of some transcription factors. PMID:21776245

  17. The anti-oxidant properties of isothiocyanates: a review.

    PubMed

    de Figueiredo, Sônia M; Filho, Sidney A V; Nogueira-Machado, José A; Caligiorne, Rachel B

    2013-09-01

    Cruciferous vegetables, such as broccoli and watercress, have been studied extensively aiming to evaluate their chemopreventive properties. Some of them have already been established using animal models. The ITCs induce Phase II enzymes related to detoxification processes of chemical carcinogens to prevent the start of carcinogenesis. They also exhibit antitumor activity at post-initiation phase, suggesting their additional role(s) in cancer prevention. Sulforaphane is the most extensively studied isothiocyanate, focused in its anti-tumoral activity and it is mainly found in great amounts in broccoli and other cruciferous. In a dose dependent manner, ITCs inhibit the cell viability of human cervical cancer cells, human pancreatic cancer cells, human hepatocellular carcinoma cells, human ovarian cancer cells, and have antiinflammatory properties in the treatment of human T-cell leukemia cells. This protective effect may be due to improved antioxidant status. Although the health effects of diet in humans are generally considered promising, there are definite challenges and limitations of the current data in better understanding of the molecular mechanisms responsible for this effect, together with the possible interactions between different dietary constituents. The survey of relevant patents on the use of isothiocyanates such as sulforaphane for cancer and cardiovascular diseases treatments is also included in this review. PMID:23978168

  18. Antioxidant properties of biohybrids based on liposomes and sage silver nanoparticles.

    PubMed

    Barbinta-Patrascu, Marcela Elisabeta; Bunghez, Ioana-Raluca; Iordache, Stefan Marian; Badea, Nicoleta; Fierascu, Radu-Claudiu; Ion, Rodica Mariana

    2013-03-01

    This paper is aimed to describe a simple and rapid eco-friendly bottom-up approach for the preparation of antioxidant silver bionanostructures using a leaf extract from sage (Salvia officinalis L.). The bioreduction property of sage in the synthesis of silver nanoparticles was investigated by UV-VIS and Attenuated Total Reflectance Fourier Transform Infrared spectroscopy. During their preparation, the particle size analysis was performed by using Dynamic Light Scattering technique. Ultrasonic irradiation was used to obtain sage silver nanoparticles. The morphology (size and shape) of the herbal silver nanoparticles was evaluated by Scanning Electron Microscopy that revealed the formation of spherical phytonanoparticles with size less than 80 nm. In order to increase their stability and their biocompatibility, the sage silver nanoparticles were introduced in two types of liposomes: soybean lecithin- and Chla-DPPC-lipid vesicles which were prepared by thin film hydration method. X-Ray Fluorescence analysis confirmed the silver presence in liposomes/sage-AgNPs biohybrids. The stability of liposomes/herbal AgNPs bioconstructs was checked by zeta potential measurements. The most stable biohybrids: Chla-DPPC/sage-AgNPs with zeta potential value of -34.2 mV, were characterized by Atomic Force Microscopy revealing the spherical and quasi-spherical shaped profiles of these nanobiohybrids with size less than 96 nm. The antioxidant activity of the silver bionanostructures was evaluated using chemiluminescence assay. The developed eco-friendly silver phytonanostructures based on lipid membranes, nanosilver and sage extract, manifest strong antioxidant properties (between 86.5% and 98.6%). PMID:23755645

  19. Effect of sorghum type and processing on the antioxidant properties of sorghum [sorghum bicolor (l.) moench] based foods 

    E-print Network

    Ngwenya, Nomusa Rhoda

    2009-05-15

    Antioxidant properties of sorghum are related to sorghum type and method of processing into foods. Tannin and non-tannin sorghums and their products were evaluated for total phenols, tannins and antioxidant activity. Total ...

  20. Gamma-irradiation improves the color and antioxidant properties of Chaga mushroom (Inonotus obliquus) extract.

    PubMed

    Kim, Jae-Hun; Sung, Nak-Yun; Kwon, Sun-Kyu; Srinivasan, Periasamy; Song, Beom-Seok; Choi, Jong-Il; Yoon, Yohan; Kim, Jin Kyu; Byun, Myung-Woo; Kim, Mee-Ree; Lee, Ju-Woon

    2009-12-01

    The objective of this study was to evaluate the effect of ionizing radiation on color and antioxidative properties of Chaga mushroom (Inonotus obliquus) extract (CME). CME (10 mg/mL) was gamma-irradiated at 0, 3, 5, 7, and 10 kGy, and color, antioxidant activity, and total phenolic compound levels were then determined. The lightness and yellowness were increased (P < .05), and the redness was decreased (P < .05), as irradiation dose increased. The antioxidant parameters such as the 2-diphenyl-1-picrylhydrazyl, superoxide, and hydroxyl radical scavenging activities, ferric reducing/antioxidant power, and inhibition of lipid peroxidation increased as the irradiation dose increased. Also, the total phenolic compound levels of CME were increased (P < .05) by gamma-irradiation. These results suggest that gamma-irradiation could be considered a means for improving the antioxidant properties and the color of CME. PMID:20041791

  1. Antioxidant properties and efficacies of synthesized alkyl caffeates, ferulates, and coumarates.

    PubMed

    Sørensen, Ann-Dorit Moltke; Durand, Erwann; Laguerre, Mickaël; Bayrasy, Christelle; Lecomte, Jérôme; Villeneuve, Pierre; Jacobsen, Charlotte

    2014-12-31

    Caffeic, ferulic, and coumaric acids were lipophilized with saturated fatty alcohols (C1-C20). The antioxidant properties of these hydroxycinnamic acids and their alkyl esters were evaluated in various assays. Furthermore, the antioxidant efficiency of the compounds was evaluated in a simple o/w microemulsion using the conjugated autoxidizable triene (CAT) assay. All evaluated phenolipids had radical scavenging, reducing power, and metal chelating properties. Only caffeic acid and caffeates were able to form a complex with iron via their catechol group in the phenolic ring. In the o/w emulsion, the medium chain phenolipids of the three homologues series were most efficient. The antioxidant properties and efficacies were dependent upon functional groups substituted to the ring structure and were in the following order: caffeic acid and caffeates > ferulic acid and ferulates > coumaric acid and coumarates. Moreover, the results demonstrated that the test system has an impact on the antioxidative properties measured. PMID:25457614

  2. Characterization and antioxidant activity of two low-molecular-weight polysaccharides purified from the fruiting bodies of Ganoderma lucidum.

    PubMed

    Liu, Wei; Wang, Hengyu; Pang, Xiubing; Yao, Wenbing; Gao, Xiangdong

    2010-05-01

    Two low-molecular-weight polysaccharides, GLP(L)1 and GLP(L)2, purified from a crude Ganoderma lucidum polysaccharide preparation GLPP were investigated for their physicochemical properties, structure characterization and antioxidant activities. The results indicated that GLP(L)1 was a glucan with an average molecular weight of 5.2kDa, while GLP(L)2 was composed of glucose, galactose and mannose in a ratio of 29:1.8:1.0 with the average molecular weight of 15.4kDa. GLP(L)1 and GLP(L)2 had similar structure characteristic which contained linkages such as -->3)-Glcp-(1-->, -->4)-Glcp-(1-->, -->6)-Glcp-(1-->, -->3,6)-Glcp-(1-->, and -->4,6)-Glcp-(1--> in the percentage ratio of 21.9:20.3:23.7:24.0:3.7 and 23.0:34.6:7.0:14.1:3.0 in the backbone or branches, respectively. Antioxidant results showed that both GLP(L)1 and GLP(L)2 exhibited antioxidant activities while GLP(L)1 was more effective in free radicals scavenging and Fe(2+) chelating. Low-molecular-weight polysaccharide seems to play an important role in the exploration of natural antioxidants in food industry and pharmaceuticals. PMID:20153767

  3. Antioxidant and anti-inflammatory properties of C-phycocyanin from blue-green algae

    Microsoft Academic Search

    C. Romay; J. Armesto; D. Remirez; R. González; N. Ledon; I. García

    1998-01-01

    Objective: Phycocyanin is a pigment found in blue-green algae which contains open chain tetrapyrroles with possible scavenging properties. We have studied its antioxidant properties.¶Materials and methods: Phycocyanin was evaluated as a putative antioxidant in vitro by using: a) luminol-enhanced chemiluminescence (LCL) generated by three different radical species (Oф, OH”, RO”) and by zymosan activated human polymorphonuclear leukocytes (PMNLs), b) deoxyribose

  4. In vitro studies indicating antioxidative properties of rebamipide.

    PubMed

    Iinuma, S; Naito, Y; Yoshikawa, T; Takahashi, S; Takemura, T; Yoshida, N; Kondo, M

    1998-09-01

    Rebamipide is the first anti-gastric ulcer and antigastritis drug that not only increases endogenous prostaglandin in gastric mucosa but also scavenges oxygen-derived free radicals and inhibits their production. In the present paper, we have reviewed the antioxidative and antiinflammatory properties of rebamipide mainly demonstrated by in vitro studies. The study, using the electron paramagnetic resonance (EPR) spin trapping technique, showed that superoxide production was inhibited by rebamipide when isolated human neutrophils were stimulated with opsonized zymosan or Helicobacter pylori water extract in a dose-dependent manner. Chemiluminescence generated from neutrophils activated by H. pylori or formyl-methionyl-leucyl-phenylalanine was also decreased by the treatment with rebamipide. Rebamipide, at concentrations of 10(-5) and 10(-6) M, reduced the adherence of neutrophils to endothelial cells as well as the CD18 expression on neutrophils induced by H. pylori water extract. The EPR study also demonstrated the direct hydroxyl radical scavenging activity of rebamipide, and a kinetic study showed that the second-order rate constant for the reaction between rebamipide and hydroxyl radical was 2.24 x 10(10) M(-1)/s(-1). The inhibitory effect of rebamipide on lipid peroxidation induced by a free radical initiator was also demonstrated by the in vitro system using rat gastric mucosal homogenates. These data indicate that rebamipide offers a potential for protection against reactive oxygen- and activated neutrophil-associated gastric mucosal injury by scavenging hydroxyl radical and inhibiting neutrophil activation or lipid peroxidation. PMID:9753224

  5. Antioxidant and antimicrobial properties of some lichens and their constituents.

    PubMed

    Kosani?, Marijana; Rankovi?, Branislav

    2011-12-01

    The aim of this study is to examine the in vitro antioxidant and antimicrobial activities of the acetone, methanol, and aqueous extracts of the lichens Cladonia furcata (many-forked Cladonia), Hypogymnia physodes (puffed lichen), and Umbilicaria polyphylla (manyleaf navel lichen) and the antimicrobial activity of fumarprotocetraric acid, gyrophoric acid, and physodic acid isolated from the respective lichen species. Antioxidant activity was evaluated by five different methods: free radical scavenging, superoxide anion radical scavenging, reducing power, determination of total phenolic compounds, and determination of total flavonoid content. U. polyphyla exhibited more powerful antioxidant activities than the other lichen species examined: methanol extracts of U. polyphyla showed 90.08% free radical scavenging activities (the ratio was greater than those of standard antioxidants). Moreover, the extracts tested had effective reducing power and superoxide anion radical scavenging. Total contents of phenol and flavonoid in extracts were determined as pyrocatechol equivalents and as rutin equivalents, respectively. A significant strong relationship between total phenolic and flavonoid contents and their antioxidative activities was observed. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method against six species of bacteria and 10 species of fungi. In general, the lichen extracts tested and their respective acids had relatively strong antimicrobial activity against the microorganisms tested. The present study shows that the lichen species tested demonstrated strong antioxidant and antimicrobial activities. The results suggest that lichens may be used as good sources of natural antioxidants and for pharmaceutical purposes in treating various diseases. PMID:21861720

  6. Evaluation of Garlic Cultivars for Polyphenolic Content and Antioxidant Properties

    PubMed Central

    Cheng, Siqiong; Li, Panpan; Du, Junna; Chang, Yanxia; Meng, Huanwen

    2013-01-01

    Two phenolic compound parameters (total phenolic and flavonoid contents) and 5 antioxidant parameters (DPPH [2, 2-diphenyl-1-picrylhydrazyl] radical scavenging activity, HRSC (hydroxyl radical scavenging capacity), FRAP (ferric ion reducing antioxidant power), CUPRAC (cupric ion reducing antioxidant capacity), and MCA (metal chelating activity) were measured in bulbs and bolts of 43 garlic cultivars. The bulbs of cultivar ‘74-x’ had the highest phenolic content (total phenolic, flavonoids) and the strongest antioxidant capacity (DPPH, FRAP, and CUPRAC), followed by bulbs of cultivar ‘Hanzhong purple’; the bulbs of cultivar ‘Gailiang’ had the lowest phenolic content and antioxidant capacity (FRAP, CUPRAC, MCA). The bolts of ‘Hanzhong purple’ also had higher phenolic content. Principal components analysis (PCA) separated the cultivars into 3 groups according to phenolic and flavonoid contents and strength of antioxidant activity. The first group had higher HRSC, FRAP, and flavonoid content; the second group had higher total phenolic content and MCA; some cultivars in the third group had higher HRSC and FRAP. All 8 test garlic bulb extracts successfully prevented Human Vascular Endothelial Cell death and significantly prevented reactive-oxygen species (ROS) formation in oxidative stress model, in which cultivar ‘74-x’ had highest protection capability, following by cultivar ‘Hanzhong purple’, and the bulbs of cultivar ‘No. 105 from Korea’ had the lower protection capability against cell death and ROS formation. The protection capability in vivo of these garlic cultivars was consistent with their phenolic content and antioxidant capacity. PMID:24232741

  7. Novel conductive polypyrrole/zinc oxide/chitosan bionanocomposite: synthesis, characterization, antioxidant, and antibacterial activities.

    PubMed

    Ebrahimiasl, Saeideh; Zakaria, Azmi; Kassim, Anuar; Basri, Sri Norleha

    2015-01-01

    An antibacterial and conductive bionanocomposite (BNC) film consisting of polypyrrole (Ppy), zinc oxide (ZnO) nanoparticles (NPs), and chitosan (CS) was electrochemically synthesized on indium tin oxide (ITO) glass substrate by electrooxidation of 0.1 M pyrrole in aqueous solution containing appropriate amounts of ZnO NPs uniformly dispersed in CS. This method enables the room temperature electrosynthesis of BNC film consisting of ZnO NPs incorporated within the growing Ppy/CS composite. The morphology of Ppy/ZnO/CS BNC was characterized by scanning electron microscopy. ITO-Ppy/CS and ITO-Ppy/ZnO/CS bioelectrodes were characterized using the Fourier transform infrared technique, X-ray diffraction, and thermogravimetric analysis. The electrical conductivity of nanocomposites was investigated by a four-probe method. The prepared nanocomposites were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay. The results demonstrated that the antioxidant activity of nanocomposites increased remarkably by addition of ZnO NPs. The electrical conductivity of films showed a sudden decrease for lower weight ratios of ZnO NPs (5 wt%), while it was increased gradually for higher ratios (10, 15, and 20 wt%). The nanocomposites were analyzed for antibacterial activity against Gram-positive and Gram-negative bacteria. The results indicated that the synthesized BNC is effective against all of the studied bacteria, and its effectiveness is higher for Pseudomonas aeruginosa. The thermal stability and physical properties of BNC films were increased by an increase in the weight ratio of ZnO NPs, promising novel applications for the electrically conductive polysaccharide-based nanocomposites, particularly those that may exploit the antimicrobial nature of Ppy/ZnO/CS BNCs. PMID:25565815

  8. Novel conductive polypyrrole/zinc oxide/chitosan bionanocomposite: synthesis, characterization, antioxidant, and antibacterial activities

    PubMed Central

    Ebrahimiasl, Saeideh; Zakaria, Azmi; Kassim, Anuar; Basri, Sri Norleha

    2015-01-01

    An antibacterial and conductive bionanocomposite (BNC) film consisting of polypyrrole (Ppy), zinc oxide (ZnO) nanoparticles (NPs), and chitosan (CS) was electrochemically synthesized on indium tin oxide (ITO) glass substrate by electrooxidation of 0.1 M pyrrole in aqueous solution containing appropriate amounts of ZnO NPs uniformly dispersed in CS. This method enables the room temperature electrosynthesis of BNC film consisting of ZnO NPs incorporated within the growing Ppy/CS composite. The morphology of Ppy/ZnO/CS BNC was characterized by scanning electron microscopy. ITO–Ppy/CS and ITO–Ppy/ZnO/CS bioelectrodes were characterized using the Fourier transform infrared technique, X-ray diffraction, and thermogravimetric analysis. The electrical conductivity of nanocomposites was investigated by a four-probe method. The prepared nanocomposites were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay. The results demonstrated that the antioxidant activity of nanocomposites increased remarkably by addition of ZnO NPs. The electrical conductivity of films showed a sudden decrease for lower weight ratios of ZnO NPs (5 wt%), while it was increased gradually for higher ratios (10, 15, and 20 wt%). The nanocomposites were analyzed for antibacterial activity against Gram-positive and Gram-negative bacteria. The results indicated that the synthesized BNC is effective against all of the studied bacteria, and its effectiveness is higher for Pseudomonas aeruginosa. The thermal stability and physical properties of BNC films were increased by an increase in the weight ratio of ZnO NPs, promising novel applications for the electrically conductive polysaccharide-based nanocomposites, particularly those that may exploit the antimicrobial nature of Ppy/ZnO/CS BNCs. PMID:25565815

  9. Antioxidant properties of various solvent extracts from purple basil

    NASA Astrophysics Data System (ADS)

    Ye?ilo?lu, Ye?im; ?it, Latif?ah

    2012-09-01

    Water, ethanol and acetone extracts from leaves and flowers of purple basil, one of the most popular spices consumed in the Thrace region of Turkey, were tested in vitro for their ability to inhibit peroxidation of lipids, to scavenge DPPH, hydrogen peroxide, superoxide anion, to reduce Fe(III) to Fe(II) and to chelate Fe(II) ions. The results showed that purple basil contained naturally occurring antioxidant components and possessed antioxidant activity which may be attributed to its lipid peroxidation inhibitory, radical scavenging and metal chelating activities. It was concluded that purple basil might be a potential source of antioxidants.

  10. In Vitro Antibacterial, Antifungal, Antibiofilm, Antioxidant, and Anticancer Properties of Isosteviol Isolated from Endangered Medicinal Plant Pittosporum tetraspermum

    PubMed Central

    Valan Arasu, Mariadhas; Rejiniemon, Thankappan Sarasam

    2015-01-01

    This study aimed to investigate the in vitro antibacterial, antifungal, antibiofilm, antioxidant, and anticancer properties of isosteviol isolated from endangered medicinal plant Pittosporum tetraspermum. Pure compound was obtained and characterized by column chromatography followed by 1H NMR, 13C NMR, IR, and mass spectral analysis. The antimicrobial activities of the compound were assessed by the broth microdilution method and the antioxidant properties were determined using reducing ability assay, DPPH scavenging assay, hydroxyl radical scavenging activity, and superoxide radical scavenging assay. Anticancer study was evaluated by following MTT assay. Column purification and spectrocopical analysis lead to identifying isosteviol from the crude ethyl acetate extract. The compound exhibited significant activity against bacteria such as Staphylococcus epidermidis (125?µg/mL), Staphylococcus aureus (125?µg/mL), and Klebsiella pneumoniae (62.5?µg/mL). The MIC of the compound against Candida albicans, Aspergillus niger, and Trichophyton mentagrophytes was 62.5, 125, and 500?µg/mL, respectively. The compound showed comparatively better antibiofilm activity against E. coli, S. typhi, and P. aeruginosa. Furthermore, it exhibited good antioxidant properties. Anticancer properties of the compound against Vero and MCF7 cell lines were its advantage. Novel isosteviol would be useful to reduce the infectious diseases caused by pathogenic microorganisms or slow the progress of various oxidative stress-related diseases.

  11. Antioxidant Properties of the Methanol Extract of the Wood and Pericarp of Caesalpinia decapetala

    PubMed Central

    Pawar, CR; Surana, SJ

    2010-01-01

    The antioxidant activities of the methanol extracts from the wood and pericarp of Caesalpinia decapetala (Roth) Alston (Caesalpiniaceae) were assessed in efforts to validate the herb. The antioxidant activity of the plant has been studied using its ability to scavenger DPPH, superoxide radicals, and nitric oxide radical along with its ability to inhibit lipid peroxidation. The antioxidant activity and phenolic content of the pericarp as determined by the DPPH, superoxide radical, nitric oxide radical, total phenols, the flavonoids, and total flavonols were higher than that of the wood. Analysis of plant extracts revealed a high amount of polyphenols and flavonoids suggesting a possible role of these phytoconstituents in the antioxidant property. Moreover, the results were observed in a concentration and dose dependent manner. Studies clearly indicate that the C. decapetala has significant antioxidant activity. PMID:21331190

  12. Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits of Manipur, India.

    PubMed

    Sharma, Ph Baleshwor; Handique, Pratap Jyoti; Devi, Huidrom Sunitibala

    2015-02-01

    Antioxidant properties, physico-chemical characteristics and proximate composition of five wild fruits viz., Garcinia pedunculata, Garcinia xanthochymus, Docynia indica, Rhus semialata and Averrhoa carambola grown in Manipur, India were presented in the current study. The order of the antioxidant activity and reducing power of the fruit samples was found as R. semialata?>?D. indica?>?G. xanthochymus?>?A. carambola?>?G. pedunculata. Good correlation coefficient (R(2)?>?0.99) was found among the three methods applied to determine antioxidant activity. Total phenolic content was positively correlated (R(2)?=?0.960) with the antioxidant activity however, total flavonoid content was not positively correlated with the antioxidant activity. Physico-chemical and proximate composition of these fruits is documented for the first time. PMID:25694698

  13. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers.

    PubMed

    Repetto, M G; Llesuy, S F

    2002-05-01

    There is evidence concerning the participation of reactive oxygen species in the etiology and physiopathology of human diseases, such as neurodegenerative disorders, inflammation, viral infections, autoimmune pathologies, and digestive system disorders such as gastrointestinal inflammation and gastric ulcer. The role of these reactive oxygen species in several diseases and the potential antioxidant protective effect of natural compounds on affected tissues are topics of high current interest. To consider a natural compound or a drug as an antioxidant substance it is necessary to investigate its antioxidant properties in vitro and then to evaluate its antioxidant functions in biological systems. In this review article, we shall consider the role of natural antioxidants derived from popular plants to reduce or prevent the oxidative stress in gastric ulcer induced by ethanol. PMID:12011936

  14. Chemical composition and the antioxidative properties of Nigerian Okra Seed (Abelmoschus esculentus Moench) Flour.

    PubMed

    Adelakun, O E; Oyelade, O J; Ade-Omowaye, B I O; Adeyemi, I A; Van de Venter, M

    2009-06-01

    Studies on the chemical composition and the antioxidative properties of Nigerian Okra Seed (Abelmoschus esculentus Moench) Flour were carried out. This is done to establish the nutritional composition and the antioxidative potentials of the seeds, both of which are highly implicated in health. Okra seeds were roasted at 160 degreeC for 10-60 mins. The roasted seeds were subjected to proximate, yield and antioxidative activity determination. Pre-treatment by roasting was found to increase the yield, but was found to be time dependent. The range means obtained for protein, fat, ash, fiber and sugar contents were 42.14-38.10, 31.04-17.22, 4.06-3.42, 3.45-3.60 and 8.82-8.65, respectively. The antioxidant activity was significantly increased by roasting, while in vitro digestibility showed that most antioxidative activities were available in the intestinal phase of gastrointestinal tracts. PMID:19425185

  15. Antioxidant properties of different cultivars of Portulaca oleracea

    Microsoft Academic Search

    Y. Y. Lim; E. P. L. Quah

    2007-01-01

    Methanolic extracts of six cultivars of Portulaca oleracea were analyzed for their total phenol content (TPC) using the Folin–Ciocalteu method. The antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl, ferric-reducing antioxidant power (FRAP) and ?-carotene bleaching (BCB) assays. The iodine titration method was used to determine the ascorbic acid content (AAC). The TPC of the cultivars of P. oleracea ranged from

  16. Evaluation of Antidiabetic and Antioxidant Properties of Brucea javanica Seed

    PubMed Central

    Ablat, Abdulwali; Mohamad, Jamaludin; Awang, Khalijah; Shilpi, Jamil A.; Arya, Aditya

    2014-01-01

    The ethanol extract of B. javanica seed was fractionated with solvents of different polarities and tested for antioxidant activities by several assays including DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP), ferrous ion chelating activity (FCA), and nitric oxide radical scavenging activity (NORSA) along with their polyphenolic contents. Antidiabetic activity was evaluated both in vitro and in vivo using a glycogen phosphorylase ? (GP?) inhibition assay and oral glucose tolerance test (OGTT) in nondiabetic rats. The ethyl acetate fraction (EAF), rich in tannin, exhibited the strongest antioxidant activities to DPPH, FRAP, and NORSA, except for FCA. The EAF also exerted a dose-depended inhibition of GP? (IC50 = 0.75?mg/ml). Further evaluation of hypoglycemic effect on OGGT indicated that rats treated with EAF (125?mg/kg?bw) showed a 39.91% decrease (P < 0.05) in blood glucose levels at 30?min, and continuous fall (P < 0.05) of 28.89% and 20.29% was observed in the following hours (60 and 90?min) compared to the normal control during OGTT. The EAF was applied to polyamide column chromatography, and the resulting tannin-free fraction was tested for both GP? inhibition and antioxidant (DPPH only) activity. The GP? inhibitory activity was retained, while antioxidant activity was lost (4.6-fold) after tannin removal. These results concluded that the GP? inhibitory activity initially detected was primarily due to the compounds other than tannins, whereas antioxidant activity was mainly due to the tannins. PMID:24688431

  17. Insights on the formulation of herbal beverages with medicinal claims according with their antioxidant properties.

    PubMed

    Barreira, João C M; Morais, Ana L; Ferreira, Isabel C F R; Oliveira, M Beatriz P P

    2013-01-01

    Several herbal beverages claim medicinal benefits due to their antioxidant properties. However, operational factors such as the extracted herbal component, preparation method or concentration levels, might influence their biological activity. To assess this effect, the antioxidant activity of beverages prepared with Camellia sinensis, Aspalathus linearis or Cochlospermum angolensis, used solely or mixed with different fruit, plant or algae extracts, was studied using different formulations (bags, leaves, roots, granulates, powders, liquids) and different preparation methods (infusion, solubilisation or promptly used). The DF50 (dilution factor responsible for 50% of antioxidant activity) values were calculated to compare their antioxidant activity. A linear discriminant analysis was used to categorize the assayed samples according to their antioxidant activity and bioactive molecules profiles. The results indicated that antioxidant activity and antioxidant compounds are significantly affected by formulation and preparation method, but overall the labelled antioxidant benefits were validated. Green tea showed the highest activity, but with different behaviour within each used formulation. The high DF50 values calculated for some products might be used to adjust the dietary dose or formulation, preventing also putative pro-oxidant effects. Hence, the obtained results might be useful to define the formulation of these highly consumed herbal beverages, enhancing their health effects. PMID:23459297

  18. Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis.

    PubMed

    Frozza, Caroline Olivieri da Silva; Garcia, Charlene Silvestrin Celi; Gambato, Gabriela; de Souza, Marcia Denize Oliveira; Salvador, Mirian; Moura, Sidnei; Padilha, Francine Ferreira; Seixas, Fabiana Kömmling; Collares, Tiago; Borsuk, Sibele; Dellagostin, Odir Antonio; Henriques, João Antonio Pêgas; Roesch-Ely, Mariana

    2013-02-01

    Propolis is known for a long time for its health benefits and biological activities. Here, the red variety from the northeast of Brazil was chemically analyzed and extracts were investigated regarding their antioxidant and antitumor activity. Hydroalcoholic extracts, obtained from the red propolis, revealed polyphenol content, 2,2-diphenyl-1-picrylhydrazyl scavenging potential and enzymatic activities for catalase-like and superoxide dismutase-like. Cytotoxic activity was evaluated for human laryngeal epidermoid carcinoma cell (Hep-2), human cervical adenocarcinoma (HeLa) and human normal epithelial embryonic kidney (Hek-293). Survival analysis for non-tumor cell line showed greater IC50 compared to tumor cell lines, suggesting an increased sensitivity that may correlate with the higher proliferative index of the tumor vs. normal cells. Our results indicate that the Brazilian red propolis is capable of inhibiting cancer cell growth and constitutes an excellent source of antioxidant and antitumor natural agent. PMID:23174518

  19. Evaluation of antioxidative properties of Geranium macrorrhizum and Potentilla fruticosa extracts in Dutch style fermented sausages.

    PubMed

    Miliauskas, G; Mulder, E; Linssen, J P H; Houben, J H; van Beek, T A; Venskutonis, P R

    2007-12-01

    Antioxidative properties of Geranium macrorrhizum, Potentilla fruticosa and Rosmarinus officinalis (as a reference) extracts were evaluated in Dutch style fermented sausages. Extracts were incorporated into sausages during preparation. The sausages were subsequently fermented, tested and compared to a standard spices mix, traditionally used for the production of such sausages. Formation of the primary oxidation products - peroxides, and secondary - TBARS and hexanal was monitored. The polar extracts from Potentilla showed some antioxidant activity, especially in combination with ascorbate, however the activity was low compared to the standard spices mix. Polar extracts from Geranium showed only negligible antioxidant activity. PMID:22061961

  20. Characterization and antioxidant activity in vitro and in vivo of polysaccharide purified from Rana chensinensis skin.

    PubMed

    Wang, Zhanyong; Zhao, Yuanyuan; Su, Tingting; Zhang, Jing; Wang, Fei

    2015-08-01

    Preliminary characterization and antioxidant activity in vitro and in vivo investigation of the polysaccharide fraction named as RCSP II, which was extracted from Rana chensinensis skin, were performed. Results indicated that RCSP II comprised glucose, galactose, and mannose in a molar ratio of 87.82:2.77:1.54 with a molecular weight of 12.8kDa. Antioxidant activity assay in vitro showed that RCSP II exhibited 75.2% scavenging activity against 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) radicals at the concentration of 2500mg/L and 85.1% against chelated ferrous ion at 4000mg/L. Antioxidant activity assay in vivo further showed that RCSP II increased the activities of antioxidant enzymes, decreased the levels of malondialodehyde, and enhanced total antioxidant capabilities in livers and sera of d-galactose induced mice. These results suggested that RCSP II could have potential antioxidant applications as medicine or functional food. PMID:25933517

  1. Antioxidant properties of Mediterranean food plant extracts: geographical differences.

    PubMed

    Schaffer, S; Schmitt-Schillig, S; Müller, W E; Eckert, G P

    2005-03-01

    Locally grown, wild food plants seasonally contribute a considerable portion of the daily diet in certain Mediterranean areas and it has been suggested that the beneficial effects of the Mediterranean diet on human health partly originate from the antioxidant effect of flavonoid-rich food plants. The nutrient content of most wild plants is higher than that of cultivated ones and may vary depending on the prevailing environmental conditions. Accordingly, three local Mediterranean plant foods (i.e. Cichorium intybus, Sonchus oleraceus, Papaver rhoeas) were collected in Greece (Crete), southern Italy, and southern Spain in order to assess possible differences in their in vitro antioxidant potential. The biological assays revealed diverse intra-plant specific antioxidant effects for the tested extracts ranging from no activity to almost complete protection. Furthermore, substantial differences in the polyphenol content were found for the nutritionally used part of the same plant originating from different locations. However, no clear correlations between the polyphenol content and the extracts' antioxidant activities were found. Taken together, the data suggest that certain local Mediterranean plant foods possess promising antioxidant activity and that the observed biological effects are possibly influenced by the geographically-dependent environmental conditions prevailing during plant growth. PMID:15800389

  2. Screening of antioxidant properties of seven Umbelliferae fruits from Iran.

    PubMed

    Nickavar, Bahman; Abolhasani, Farideh Al-Sadat

    2009-01-01

    Antioxidative activities (IC(50)) of ethanol extracts from seven Umbelliferae fruits (Bunium persicum, Coriandrum sativum, Cuminum cyminum, Foeniculum vulgare, Heracleum persicum, Pimpinella anisum and Trachyspermum copticum) have been studied by the DPPH (2,2'-diphenyl-1-picrylhydrazyl) radical scavenging test. All the studied extracts showed antioxidant capability and P. anisum extract exhibited the strongest activity. The scavenging activity of the extracts in decreasing order was: P. anisum> T. copticum> C. cyminum> F. vulgare > or = B. persicum > or = C. sativum> H. persicum. The extracts were also investigated regarding their total flavonoid contents by the AlCl3 technique. The decreasing order of the flavonoid content of the extracts was: C. cyminum > T. copticum> P. anisum > or = H. persicum > or = B. persicum > or = F. vulgare > or = C. sativum. However, a favorable correlation was not found between the antioxidant activity and the total flavonoid content of the extracts. As well, the most active extract (i.e. P. anisum) was partitioned with n-hexane, chloroform and ethyl acetate to yield three organic fractions together with the remaining aqueous fraction. The antioxidative activities (IP%) and flavonoid contents of the fractions were also determined. The ethyl acetate fraction exhibited the highest activity and content. A positive correlation was found between the antioxidant potency and flavonoid content of the fractions. PMID:19168417

  3. Herbal remedies of Solidago—correlation of phytochemical characteristics and antioxidative properties

    Microsoft Academic Search

    P Apáti; K Szentmihályi; Sz. T Kristó; I Papp; P Vinkler; É Szoke; Á Kéry

    2003-01-01

    In this study the correlation of phytochemical characteristics and antioxidative properties of classical herbal tea extracts—Infusum solidaginis, Decoctum solidaginis, Maceratum solidaginis—and tinctures prepared by various concentration of ethanol (40, 70, 96% v\\/v) have been examined for the release of flavonoids and their antioxidant activity. Quantitative and composition determination of flavonoids were carried out by spectrophotometry, high-performance liquid chromatography and capillary

  4. Localization of 28-kDa peroxiredoxin in rat epithelial tissues and its antioxidant properties

    Microsoft Academic Search

    S. V. Novoselov; I. V. Peshenko; V. I. Popov; V. I Novoselov; M. F. Bystrova; V. J. Evdokimov; S. S. Kamzalov; M. I. Merkulova; T. M. Shuvaeva; V. M. Lipkin; E. E. Fesenko

    1999-01-01

    Peroxiredoxins are a novel family of antioxidant proteins that specifically prevent enzymes from metal-catalyzed oxidation. The localization of a member of the mono-cystein subfamily of peroxiredoxins, the 28-kDa protein, in different rat tissues and its antioxidant properties were investigated. By immunoblotting, the 28-kDa peroxiredoxin was found to be most highly concentrated in olfactory epithelium and present in all tissues tested

  5. [Antioxidant properties of 3-oxypyridine analogues: mexidol, emoxipin, proxipin].

    PubMed

    Klebanov, G I; Liubitski?, O B; Vasil'eva, O V; Klimov, Iu V; Penzulaeva, O B; Tepliashin, A S; Tolstykh, M P; Promorenko, V K; Vladimirov, Iu A

    2001-01-01

    Using three chemiluminescent model systems of oxidation (suspension of phospholipid liposomes, a geous solution of haemoglobin-hydrogen peroxide-luminol and a geous solution 2,2'-azo-bis-(2-methylpropionamidine)dihydrochloride-luminol) the antioxidant activity and mechanism of antioxidant action of three 3-oxypyridine analogues: (mexidol, emoxipin and proxipin) were studied. These compounds were shown: a) to interact with catalitically active two valency iron ions (Fe2+), that causes elimination of ions from the model system; b) to scavenge reactive oxygen species and/or luminol radicals produced in the model systems. Their activity reduced in the following order: mexidol > emoxipin > proxipin. The antioxidant activity of 3-oxypyridines may underline known clinical effects of these compounds. PMID:11558311

  6. Plant derived and dietary phenolic antioxidants: anticancer properties.

    PubMed

    Roleira, Fernanda M F; Tavares-da-Silva, Elisiário J; Varela, Carla L; Costa, Saul C; Silva, Tiago; Garrido, Jorge; Borges, Fernanda

    2015-09-15

    In this paper, a review of the literature on the phenolic compounds with anticancer activity published between 2008 and 2012 is presented. In this overview only phenolic antioxidant compounds that display significant anticancer activity have been described. In the first part of this review, the oxidative and nitrosative stress relation with cancer are described. In the second part, the plant-derived food extracts, containing identified phenolic antioxidants, the phenolic antioxidants isolated from plants and plant-derived food or commercially available and the synthetic ones, along with the type of cancer and cells where they exert anticancer activity, are described and summarized in tables. The principal mechanisms for their anti-proliferative effects were also described. Finally, a critical analysis of the studies and directions for future research are included in the conclusion. PMID:25863633

  7. Phenolics, antioxidative activity and characterization of anthocyanins in berries of blackcurrant interspecific hybrids.

    PubMed

    Anisimovien?, Nijol?; Jankauskien?, Jurga; Jodinskien?, Milda; Bendokas, Vidmantas; Stanys, Vidmantas; Šikšnianas, Tadeušas

    2013-01-01

    The interest in phenols and anthocyanins has increased due to their antioxidant properties and to their potential usage as dietary antioxidants in human nutrition. Total phenols and anthocyanin content, composition and stability in berry extracts of blackcurrant interspecific hybrids, and antioxidative activity of extracts was evaluated. Berries of interspecific hybrids accumulated 530 to 614 mg 100 g(-1) FW of total phenolic compounds, while 621 mg 100 g(-1) FW of phenolics was established in berries of control Ribes nigrum cultivar 'Ben Tirran'. 'Ben Tirran' berries accumulated 444 mg 100 g(-1) FW of anthocyanins and higher amount was identified in berries of interspecific hybrids No. 11-13 (R. nigrum × R. petraeum) and No. 57 (R. nigrum × R. aureum), 522 and 498 mg100 g(-1) FW respectively. Berry extracts of hybrid No. 11-13 distinguished by the highest antioxidative activity (80%) and it was higher than antioxidant activity of 'Ben Tirran' (70%). Antioxidative activity of all tested berry extracts (70-80%) was twice higher compared to synthetic antioxidant BHT (39%). However correlation between phenolics or total anthocyanin content and antioxidative activity degree was not established. Amount of cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside in berries of hybrids No. 57, No. 11-13 and No. 8 ((R. nigrum × R. americanum) × (R. nigrum × R. americanum)) was higher than in berries of 'Ben Tirran'. It was established that cyanidins are more stable anthocyanins in all studied temperature and irradiation conditions. Therefore interspecific hybrids No. 57 and No. 11-13 were the most agronomically valuable hybrids. PMID:24432329

  8. Design of novel potent antihyperlipidemic agents with antioxidant/anti-inflammatory properties: exploiting phenothiazine's strong antioxidant activity.

    PubMed

    Matralis, Alexios N; Kourounakis, Angeliki P

    2014-03-27

    Because atherosclerosis is an inflammatory process involving a series of pathological events such as dyslipidemia, oxidative stress, and blood clotting mechanisms, we hereby report the synthesis and evaluation of novel compounds in which antioxidant, anti-inflammatory, and squalene synthase (SQS) inhibitory/hypolipidemic activities are combined in simple molecules through design. The coupling of two different pharmacophores afforded compounds 1-12, whose biological profile was markedly improved compared to those of parent lead structures (i.e., the hypolipidemic 2-hydroxy-2-aryl-(benzo)oxa(or thia)zine and the antioxidant phenothiazine). Most derivatives strongly inhibited in vitro microsomal lipid and LDL peroxidation, exhibiting potent free-radical scavenging activity. They further significantly inhibited SQS activity and showed remarkable antidyslipidemic activity in vivo in animal models of acute and high-fat-induced hyperlipidemia. Finally, several compounds showed anti-inflammatory activity in vitro, inhibiting cycloxygenase (COX-1/2) activity. The multimodal properties of the new compounds and especially their combined antioxidant/SQS/COX inhibitory activity render them interesting lead compounds for further evaluation against atherosclerosis. PMID:24568631

  9. Preparation and antioxidant properties of water extract of propolis

    Microsoft Academic Search

    Takeshi Nagai; Reiji Inoue; Hachiro Inoue; Nobutaka Suzuki

    2003-01-01

    A water extract was prepared from fresh propolis from Brazil. Antioxidant activity was measured using a lipid peroxidation model system. The activity was very strong and, at 1 and 5 mg\\/ml, higher than that of 5 mM ascorbic acid. The scavenging activity against superoxide anion radical of water extract of propolis was high, and the extracts, at 50 and 100

  10. Quantitative evaluation of the antioxidant properties of garlic and shallot preparations

    Microsoft Academic Search

    Nuttakaan Leelarungrayub; Viboon Rattanapanone; Nantaya Chanarat; Janusz M. Gebicki

    2006-01-01

    ObjectiveShallots and garlic are an important part of the diet of many populations and there is long-held belief in their health enhancing properties. This study tested whether fresh and commercial shallot and garlic preparations have antioxidant properties that might justify such claims.

  11. Basic nutritional investigation Quantitative evaluation of the antioxidant properties of garlic and shallot preparations

    Microsoft Academic Search

    Nuttakaan Leelarungrayub; Viboon Rattanapanone; Nantaya Chanarat; Janusz M. Gebicki

    Objective: Shallots and garlic are an important part of the diet of many populations and there is long-held belief in their health enhancing properties. This study tested whether fresh and commer- cial shallot and garlic preparations have antioxidant properties that might justify such claims. Methods: Samples produced by pressing and extraction of bulbs were tested for their ability to decrease

  12. Evidence of Possible Evolutionary Divergence in Plant Genera Based on Antioxidant Properties

    ERIC Educational Resources Information Center

    Asai, Elizabeth; Cao, Sharon

    2009-01-01

    The purpose of this investigation was to determine if three Western species of the Panax, Lycium, and Astragalus genera had antibacterial and/or antioxidant properties, and how their properties compared to Eastern herbs in the same genera. The group hypothesized that when compared, the corresponding herbs would have identical antibacterial and…

  13. Isolation, characterization and antioxidative activity of C-phycocyanin from Limnothrix sp. strain 37-2-1

    PubMed Central

    Gantar, Miroslav; Simovi?, Dragan; Djilas, Sonja; Gonzalez, Walter W.; Miksovska, Jaroslava

    2012-01-01

    C-phycocyanin (C-PC) is a blue colored accessory photosynthetic pigment found in cyanobacteria. Some of the medicinal properties of Spirulina have been attributed to this pigment, which includes anticancer, antioxidant, and anti-inflammatory activity. We have screened cyanobacteria isolated from freshwater habitats in Florida for their high content of C-PC. Of 125 strains tested, one filamentous strain identified as Limnothrix sp. was selected for further research. This strain produced 18% C-PC of total dry biomass. Here we describe a simple method for obtaining C-PC of high purity without the use of ion exchange chromatography. The procedure is based on pigment precipitation from the cell lysate with an appropriate concentration of ammonium sulfate, then purification with activated carbon and chitosan, followed by a sample concentration using tangential flow filtration. We have shown that when the lower concentration of ammonium sulfate was used, C-PC with higher purity index was recovered. Characterization of C-PC from Limnothrix showed that it had an absorbance maximum at 620 nm and fluorescence at 639 nm. The molecular mass of intact C-PC was estimated to be ~50 kDa with ? and ? subunits forming dimmers. When C-PC content per unit biomass was compared to that of marketed Spirulina powder, we found that Limnothrix was superior. C-phycocyanin from Limnothrix had an antioxidative activity on DPPH free radicals similar to that found in a natural antioxidant – rutin. PMID:22353597

  14. Antioxidative Properties and Inhibition of Key Enzymes Relevant to Type-2 Diabetes and Hypertension by Essential Oils from Black Pepper

    PubMed Central

    Oboh, Ganiyu; Ademosun, Ayokunle O.; Odubanjo, Oluwatoyin V.; Akinbola, Ifeoluwa A.

    2013-01-01

    The antioxidant properties and effect of essential oil of black pepper (Piper guineense) seeds on ?-amylase, ?-glucosidase (key enzymes linked to type-2 diabetes), and angiotensin-I converting enzyme (ACE) (key enzyme linked to hypertension) were assessed. The essential oil was obtained by hydrodistillation and dried with anhydrous Na2SO4, and the phenolic content, radical [1,1-diphenyl-2 picrylhydrazyl (DPPH), 2,2?-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO)] scavenging abilities as well as the ferric reducing antioxidant property (FRAP) and Fe2+-chelating ability of the essential oil were investigated. Furthermore, the effect on ?-amylase, ?-glucosidase, and ACE enzyme activities was also investigated. The characterization of the constituents was done using GC. The essential oil scavenged DPPH?, NO?, and ABTS? and chelated Fe2+. ?-Pinene, ?-pinene, cis-ocimene, myrcene, allo-ocimene, and 1,8-cineole were among the constituents identified by GC. The essential oil inhibited ?-amylase, ?-glucosidase, and ACE enzyme activities in concentration-dependent manners, though exhibiting a stronger inhibition of ?-glucosidase than ?-amylase activities. Conclusively, the phenolic content, antioxidant activity, and inhibition of ?-amylase, ?-glucosidase, and angiotensin-1 converting enzyme activities by the essential oil extract of black pepper could be part of the mechanism by which the essential oil could manage and/or prevent type-2 diabetes and hypertension. PMID:24348547

  15. Antioxidant property of quercetin-Cr(III) complex: The role of Cr(III) ion

    NASA Astrophysics Data System (ADS)

    Chen, Weijun; Sun, Shaofang; cao, Wei; Liang, Yan; Song, Jirong

    2009-01-01

    Flavonoid-metal complex is reported to exhibit a higher antioxidant activity than parent flavonoid. In this paper, experimental and theoretical methods are applied to study the antioxidant properties of quercetin and quercetin-Cr(III) complex, to find out the antioxidant activity variation and the role of Cr(III) ion on the antioxidant activity of the complex. Bond dissociation energy (BDE) and ionization potential (IP) of quercetin and the complex are calculated at the B3LYP/6-311++G(2d,2p)//B3LYP/LANL2DZ level. The experimental results show that the complex has a higher DPPH radical scavenging activity than quercetin. The calculated results show that the complex displays lower BDE and IP than quercetin. The IP of the complex declines obviously, indicating that the Cr (III) ion has more impact on the electron donating ability than on the hydrogen atom transferring ability of the complex.

  16. Effects of ?-irradiation on phenolics content, antioxidant activity and physicochemical properties of whole grainrice

    NASA Astrophysics Data System (ADS)

    Shao, Yafang; Tang, Fufu; Xu, Feifei; Wang, Yuefei; Bao, Jinsong

    2013-04-01

    Three rice genotypes with different color were gamma irradiated at a dose of 2, 4, 6, 8and 10 kGy. The aim of this study was to investigate the effect of gamma irradiation on the phenolics content and the antioxidant activity, as well as physicochemical properties of whole grain rice. The bound phenolics content in all the genotypes were significantly increased with the increase of dose of irradiation. Gamma irradiation at high dose significantly increased the free, bound and total antioxidant activities of three rice genotypes except for the free antioxidant activities of red rice. Though the color parameters were slightly changed, these changes could not be visibly identified. Rapid visco-analyzer (RVA) viscosities and gel hardness decreased continuously with the increase of the irradiation doses. It is suggested that gamma irradiation enhanced the antioxidant potential and eating quality of whole grainrice.

  17. Antioxidant properties of pyroligneous acid obtained by thermochemical conversion of Schisandra chinensis Baill.

    PubMed

    Ma, Chunhui; Li, Wei; Zu, Yuangang; Yang, Lei; Li, Jian

    2014-01-01

    Sustainable development of renewable resources is a major challenge globally. Biomass is an important renewable energy source and an alternative to fossil fuels. Pyrolysis of biomass is a promising method for simultaneous production of biochar, bio-oil, pyroligneous acid (PA), and gaseous fuels. The purpose of this study was to investigate the pyrolysis process and products yields of Schisandra chinensis fruits with different pyrolysis powers. The obtained PA was extracted with organic solvents, including ethyl formate, dichloromethane, methanol and tetrahydrofuran. The antioxidant activities, including the free radical scavenging activity and ferric reducing power, of the PA extracts were investigated. The synthetic antioxidants butylated hydroxyanisole and butylated hydroxytoluene were used as positive controls. A dichloromethane extract of PA showed excellent antioxidant properties compared to the other extracts. The chemical compositions of the PA extracts were determined by GC-MS, and further proved that the dichloromethane extract had the best antioxidant characteristics among the extracts tested. PMID:25514224

  18. Properties of Bread Dough with Added Fiber Polysaccharides and Phenolic Antioxidants: A Review

    PubMed Central

    Sivam, Anusooya S; Sun-Waterhouse, Dongxiao; Quek, SiewYoung; Perera, Conrad O

    2010-01-01

    During breadmaking, different ingredients are used to ensure the development of a continuous protein network that is essential for bread quality. Interests in incorporating bioactive ingredients such as dietary fiber (DF) and phenolic antioxidants into popular foods such as bread have grown rapidly, due to the increased consumer health awareness. The added bioactive ingredients may or may not promote the protein cross-links. Appropriate cross-links among wheat proteins, fiber polysaccharides, and phenolic antioxidants could be the most critical factor for bread dough enhanced with DF and phenolic antioxidants. Such cross-links may influence the structure and properties of a bread system during baking. This article presents a brief overview of our current knowledge of the fate of the key components (wheat proteins, fibers, and phenolic antioxidants) and how they might interact during bread dough development and baking. PMID:21535512

  19. Antioxidant and cytoprotective properties of infusions from leaves and inflorescences of Achillea collina Becker ex Rchb.

    PubMed

    Giorgi, Annamaria; Bombelli, Raffaella; Luini, Alessandra; Speranza, Giovanna; Cosentino, Marco; Lecchini, Sergio; Cocucci, Maurizio

    2009-04-01

    Plants are the main source of molecules with antioxidant and radical scavenging properties that aid the natural defence systems of cells and may be involved in the preservation of human health, particularly preventing all the physiopathological conditions where oxidative damage is a hallmark. Achillea collina Becker ex Rchb. is a medicinal plant of the Achillea millefolium aggregate (yarrow) traditionally used, particularly in mountain areas, as an infusion or alcohol extract for its digestive, antiinflammatory, analgesic, antipyretic and wound healing properties. The aim of this study was to investigate the antioxidant capacity and cytoprotective activity against oxidative stress of infusions obtained from the leaves and inflorescences of Achillea collina Becker ex Rchb., assessed by chemical (free radical scavenging activity by DPPH and Folin Ciocalteu assay) and biological assays (in vitro model of cytotoxicity and lipid peroxidation in PC12 cells line). Infusions of leaves had the highest antioxidant properties and cytoprotective activity. The antioxidant capacity was significantly correlated with the total phenolic content but not with the cytoprotective profile. Achillea collina Becker ex Rchb. has good antioxidant and cytoprotective properties, suggesting further investigations on its chemical composition and potential health value, particularly for traditionally prepared infusions of leaves. PMID:19067389

  20. Synthesis, characterization and antioxidant activity copper-quercetin complex

    NASA Astrophysics Data System (ADS)

    Bukhari, S. Birjees; Memon, Shahabuddin; Mahroof-Tahir, M.; Bhanger, M. I.

    2009-01-01

    Quercetin (3,3',4',5,7-pentahydroxyflavone) one of the most abundant dietary flavonoids, has been investigated in the presence of Cu(II) in methanol. The spectroscopic studies (UV-vis, 1H NMR and IR) were useful to assess the relevant interaction of Quercetin with Cu(II) ions, the chelation sites and dependence of the complex structure from the metal/ligand ratio. A 1:2 (L:M) complex was indicated by Job's method of continuous variation, which was applied to ascertain the stoichiometric composition of the complex. The antioxidant activities of the compounds were evaluated by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The complexed flavonoid was much more effective free radical scavengers than the free flavonoids.

  1. Synthesis and characterization of nano-encapsulated black pepper oleoresin using hydroxypropyl beta-cyclodextrin for antioxidant and antimicrobial applications.

    PubMed

    Teixeira, Bruna N; Ozdemir, Necla; Hill, Laura E; Gomes, Carmen L

    2013-12-01

    Previous studies have reported antimicrobial and antioxidant activity of black pepper oleoresin which is associated to its phenolic compounds and piperine. The ability of cyclodextrins to form an inclusion complex with a guest molecule could improve black pepper oleoresin application, bioavailability, and stability in foods. Hydroxypropyl beta-cyclodextrin (HPBCD) inclusion complex with black pepper olereosin were synthesized using the kneading method and characterized for its physico-chemical properties and its antioxidant and antimicrobial activities. Inclusion complex size was 103.9 ± 7.6 nm and indicated to be a polydisperse system. The entrapment efficiency was 78.3 ± 3.6%, which suggests that other constituents in black pepper oleoresin have higher affinities for HPBCD than piperine (major compound in black pepper oleoresin). Thermograms showed the disappearance of oxidation peaks of black pepper oleoresin, proving complex formation with HPBCD. Phase solubility results indicated 1:1 stoichiometric inclusion complex formation and an increase of black pepper oleoresin aqueous solubility with HPBCD concentration. Nano-encapsulation with HPBCD did not affect (P > 0.05) total phenolic content; however, it enhanced (P < 0.05) black pepper oleoresin antioxidant activity. Black pepper oleoresin and its inclusion complex were analyzed for their antimicrobial activity against Escherichia coli K12 and Salmonella enterica serovar Typhimurium LT2. Both free and encapsulated black pepper oleoresin effectively inhibited bacterial growth within the concentration range tested. Black pepper oleoresin encapsulated in HPBCD was able to inhibit Salmonella at lower (P < 0.05) concentrations than its corresponding free extract. Therefore, black pepper oleoresin-HPBCD nanocapsules could have important applications in the food industry as antimicrobial and antioxidant system. PMID:24329956

  2. A magnetically drivable nanovehicle for curcumin with antioxidant capacity and MRI relaxation properties.

    PubMed

    Magro, Massimiliano; Campos, René; Baratella, Davide; Lima, Giuseppina; Holà, Katerina; Divoky, Clemens; Stollberger, Rudolf; Malina, Ondrej; Aparicio, Claudia; Zoppellaro, Giorgio; Zbo?il, Radek; Vianello, Fabio

    2014-09-01

    Curcumin possesses wide-ranging anti-inflammatory and anti-cancer properties and its biological activity can be linked to its potent antioxidant capacity. Superparamagnetic maghemite (?-Fe2 O3 ), called surface-active maghemite nanoparticles (SAMNs) were surface-modified with curcumin molecules, due to the presence of under-coordinated Fe(III) atoms on the nanoparticle surface. The so-obtained curcumin-modified SAMNs (SAMN@curcumin) had a mean size of 13±4?nm. SAMN@curcumin was characterized by transmission and scanning electron microscopy, UV/Vis, FTIR, and Mössbauer spectroscopy, X-ray powder diffraction, bulk susceptibility (SQUID), and relaxometry measurements (MRI imaging). The high negative contrast proclivity of SAMN@curcumin to act as potential contrast agent in MRI screenings was also tested. Moreover, the redox properties of bound curcumin were probed by electrochemistry. SAMN@curcumin was studied in the presence of different electroactive molecules, namely hydroquinone, NADH and ferrocyanide, to assess its redox behavior. Finally, SAMN@curcumin was electrochemically probed in the presence of hydrogen peroxide, demonstrating the stability and reactivity of bound curcumin. PMID:25079005

  3. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the Limousin countryside as herbal teas

    Microsoft Academic Search

    Patrick Trouillas; Claude-Alain Calliste; Daovy-Paulette Allais; Alain Simon; Abdelghafour Marfak; Christiane Delage; Jean-Luc Duroux

    2003-01-01

    The present paper presents the antioxidant, anti-inflammatory and antiproliferative capabilities of 16 plants. These plants can be found in the Limousin countryside and most of them are used in popular medicine as herbal tea. The biological properties of the water-soluble fractions were measured. Antioxidant properties were evaluated by the ESR method in order to visualize the inhibition of the DPPH,

  4. Preparation, characterization and efficacy evaluation of synthetic biocompatible polymers linking natural antioxidants.

    PubMed

    Trombino, Sonia; Cassano, Roberta; Ferrarelli, Teresa; Leta, Sonia; Puoci, Francesco; Picci, Nevio

    2012-01-01

    The purpose of this work was the synthesis, characterization and efficacy evaluation of new biocompatible antioxidant polymers linking trans-ferulic acid or a-lipoic acid. In particular, ferulic or lipoic acid were introduced in the preformed polymeric backbone. The new antioxidant biopolymers were characterized by Fourier transform infrared spectroscopy and gel permeation chromatography. The degree of functionalization (moles of antioxidant per gram of polymer) was determined by the Gaur-Gupta method for free amino group determination and by the Folin method for the phenolic groups. Their ability to inhibit lipid peroxidation were estimated in rat liver microsomal membranes induced in vitro by tert-BOOH (tert-butyl hydroperoxide), as a source of free radicals. The DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging effect was also evaluated. The obtained systems, with different solubility, showed strong antioxidant and antiradical activities, suggesting potential use as packaging materials for foods, cosmetics, pharmaceuticals and personal care products. Moreover, the cytotoxicity of the synthesized polymers was also evaluated on Caco-2 cell cultures in order to verify their biocompatibility when exposed to an absorptive epithelial cell line. PMID:23103536

  5. [Comparative characteristics of antioxidant properties of hypoglycemic agents diabenol and gliclazide].

    PubMed

    Spasov, A A; Kosolapov, V A; Chepliaeva, N I

    2011-01-01

    The antioxidant properties of diabenol and gliclazide with reference to mexidol have been studied in vitro on several model systems, including chemiluminescence (CL) of lipids, CL with generation of reactive oxygen species (ROS), CL dependent on luminol oxidation by peroxy radicals, and the Glavind DPPH free-radical method. Diabenol exhibited the properties of a scavenger of superoxide anions and hydroxy and peroxy radicals in model CL systems with ROS generation. The activity of diabenol in inactivating ROS was about 8 times higher compared to mexidol. However, the antioxidant activity of diabenol in CL with peroxy radical generation was 3 times lower compared to mexidol. Gliclazide demonstrated dose-dependent antioxidant activity only on the model with stable DPPH radicals, where its inhibitory effect was 15 times greater than that of the reference drug. PMID:22288154

  6. Phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata Willd. subsp. mucronata Willd

    PubMed Central

    2011-01-01

    Background Several plants traditionally used in treatment of a variety of infections in South Africa are reported in ethnobotanical surveys. Many of these plants including Ziziphus mucronata subsp. mucronata lack scientific reports to support their medicinal importance. Methods The antioxidant activities and phenolic contents of the acetone, ethanol and aqueous extracts of the stems of Z. mucronata subsp. mucronata were evaluated using in vitro standard methods. The total phenol, total flavonoids and proanthocyanidin content were determined spectrophotometrically. Quercetin, Tannic acid and catechin equivalents were used for these parameters. The antioxidant activities of the stem bark extracts of this plant were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Results The quantity of the phenolic compounds, flavonoids and proanthocyanidins detected differ significantly in the various extracts. The phenolics were significantly higher than the flavonoids and proanthocyanidin contents in all the extracts investigated. The ferric reducing ability and the radical scavenging activities of the extracts were very high and dose-dependent. The ethanol extract had the highest antioxidant activity, followed by the acetone extract while the aqueous extract was the least active. Reacting with ABTS, the 50% inhibitory concentrations (IC50) were (0.0429 ± 0.04 mg/ml) for aqueous, (0.0317 ± 0.04 mg/ml) for acetone and (0.0306 ± 0.04 mg/ml) for ethanol extracts while they inhibited DPPH radical with 50% inhibitory concentration (IC50) values of 0.0646 ± 0.02 mg/ml (aqueous), 0.0482 ± 0.02 mg/ml (acetone) and 0.0422 ± 0.03 mg/ml (ethanol). Conclusions A correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant. This study, therefore, demonstrated that Z. mucronata subsp. mucronata has strong antioxidant property and free radical scavenging capability. PMID:22176659

  7. Evaluation of antioxidant and antiradical properties of Pomegranate (Punica granatum L.) seed and defatted seed extracts.

    PubMed

    Basiri, Shadi

    2015-02-01

    Pomegranate seeds are byproducts of the Pomegranate juice industries that contains functional compounds such as phenols. This study was aimed to evaluate the effect of solvents on extraction from Pomegranate seed and Pomegranate defatted seed and to measure the yield extract and phenolic content and antioxidant properties. For this purpose, the seeds and defatted seeds were directly isolated from fruits and seeds by cold pressing respectively, then were crushed and extracted with different solvents, including water, Methanol, Acetone, Ethyl acetate and Hexane and finally the extracts of them were evaluted. Phenolic compounds, ferric reducing-antioxidant power and radicals scavenging property of extracts were measured. The results showed the highest extraction efficiencies were for Hexane and Acetone solvents in extraction of seed and defatted seed respectively. The highest phenolic content was obtained from Methanol seed extract. Reducing activity test proved that the Methanol extracts of Pomegranate seed and Pomegranate defatted seed had the highest reducing strength. Results of radical scavenging activity were similar to reducing activity results. The order of antioxidant capacity of Pomegranate seed and Pomegranate defatted seed were found to be Methanol > Water > Acetone > Butanol > Ethyl acetate > Hexane. It can be concluded Pomegranate seed, which possesses high levels of polyphenols, can be one of the sources of the natural antioxidants. The Methanol extract had a higher antioxidant efficiency than seed and defatted seed extracts. PMID:25694727

  8. Investigation of Phytochemical and Antioxidant Properties of Methanol Extract and Fractions of Ballota limbata (Lamiaceae).

    PubMed

    Waheed, I; Ahmad, M; Syed, N-H; Ashraf, Romeeza

    2014-05-01

    Ballota limbata (Lamiaceae) has been used for its antispasmodic, antiulcer, diuretic, vermifuge and sedative effects in folk medicine. However, little is known about how does it work to produce these therapeutic actions. Present research investigated phytochemical components and antioxidant properties of methanol extract and different fractions of Ballota limbata. In this study, phytochemical investigation was done by performing different chemical tests. Here, antioxidant property of the extract and fractions was investigated by using 1,1-diphenyl-2-picryl hydrazyl radical scavenging activity, total antioxidant activity by the phosphomolybdenum method, linoleic acid peroxidation, ferric thiocyanate analysis and ferric-reducing antioxidant power. Methanol extract and fractions showed presence of numerous chemical principles including alkaloids, cardiac glycosides, tannins and flavonoids. The ethyl acetate fraction exhibited higher scavenging activity compared to the other fractions under investigation. This fraction displayed 84.16±1.02% 1,1-diphenyl-2-picryl hydrazyl radical inhibition at a dose of 60 ?g/ml. IC50 for 1,1-diphenyl-2-picryl hydrazylradical-scavenging activity was 13.53±0.22 ?g/ml, relative to the standard, butylatedhydroxytoluene, having IC50 of 12.33±0.88 ?g/ml. Thus, Ballota limbata showed significant antioxidant activity, which may contribute in the mechanism of above pharmacological actions. PMID:25035539

  9. Antioxidant Activity Degradation, Formulation Optimization, Characterization and Stability of Equisetum Arvense Extract Nanoemulsion

    Microsoft Academic Search

    C. Hernández-Jaimes; B. Fouconnier; C. Pérez-Alonso; J. L. Munguía-Guillén; E. J. Vernon-Carter

    2012-01-01

    Equisetum arvense supercritical CO2 extracts (EAE) contained an initial antioxidant activity of 10.3 mM TEAC that suffered a sharp first order kinetics decay characterized by half-life time (t1\\/2) of 1.05 ± 0.03 and 0.86 ± 0.03 weeks at 25 and 4°C respectively. The aim of the present work was to develop a nanoemulsion formulation for providing EAE protection against deleterious environmental factors and extending its

  10. Chemical characterization and antioxidant activity of an exopolysaccharide fraction isolated from Bifidobacterium animalis RH

    Microsoft Academic Search

    Rihua XuQian; Qian Shen; Xuelong Ding; Wengeng Gao; Pinglan Li

    2011-01-01

    The major exopolysaccharide fraction (EPSa) of Bifidobacterium animalis RH was purified to illustrate the chemical characterization and the antioxidant activity in vitro. The molecular weight of\\u000a EPSa was 2.31 × 104 Da determined by the gel permeation chromatography (GPC). Fourier-transform infrared spectra (FT-IR) and the monosaccharide\\u000a analysis of high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) showed that it was a heteropolysaccharide

  11. Subcritical water extraction of nutraceuticals with antioxidant activity from oregano. Chemical and functional characterization

    Microsoft Academic Search

    I. Rodríguez-Meizoso; F. R. Marin; M. Herrero; F. J. Señorans; G. Reglero; A. Cifuentes; E. Ibáñez

    2006-01-01

    In the present work, oregano leaves (Origanum vulgare L.) are explored as natural source of nutraceuticals with antioxidant activity. To do this, subcritical water extraction (SWE), a new environmentally friendly technique, is employed as extraction procedure and HPLC coupled to DAD is used for the chemical characterization of the extracts. Moreover, the radical scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the determination

  12. Inhibitory effect of raspberries on starch digestive enzyme and their antioxidant properties and phenolic composition

    Microsoft Academic Search

    Lei Zhang; Jianrong Li; Shelly Hogan; Hyun Chung; Gregory E. Welbaum; Kequan Zhou

    2010-01-01

    Seven primocane fall-bearing raspberry (Rubus idaeus L.) cultivars, Nova (red), Dinkum (red), Heritage (red), Autumn Britten (red), Josephine, Anne (yellow), Fall Gold (yellow) were analysed for potential health promoting properties including their inhibitory effect on starch and fat digestive enzymes, antioxidant activities, and phenolic composition. The tested raspberry extracts showed no detectable inhibition of pancreatic ?-amylase and lipase. However, all

  13. Nutritive Value and Antioxidant Properties of Cereal Gruels Produced from Fermented Maize and Sorghum

    Microsoft Academic Search

    G. Oboh; T. V. Amusan

    2009-01-01

    Cereal gruels prepared from fermented maize (yellow and white) and sorghum seeds are the forms in which cereal are popularly consumed in tropical Africa, mostly as weaning food for children. The present study sought to determine the nutritional and antioxidant properties of cereal gruels produced from maize (yellow and white) and sorghum seeds. Seeds of maize (yellow and white) and

  14. Antifungal, acetylcholinesterase inhibition, antioxidant and phytochemical properties of three Barleria species

    Microsoft Academic Search

    S. O. Amoo; A. R. Ndhlala; J. F. Finnie; J. Van Staden

    2011-01-01

    This study was aimed at evaluating the antifungal, acetylcholinesterase inhibition and antioxidant activities of petroleum ether, dichloromethane, ethanol and methanol extracts from different parts of Barleria prionitis, Barleria greenii and Barleria albostellata. Their phytochemical properties and the possibility of plant-part substitution as a conservation strategy against destructive harvesting (use of aerial parts and roots) of these species for medicinal purposes

  15. Anti-oxidant and immunomodulatory properties of seabuckthorn ( Hippophae rhamnoides)—an in vitro study

    Microsoft Academic Search

    S Geetha; M Sai Ram; V Singh; G Ilavazhagan; R. C Sawhney

    2002-01-01

    This study was designed to determine the anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides) using lymphocytes as a model system. Chromium(VI) as potassium dichromate was used to induce oxidative damage. The production of free radicals by chromium and the ability of alcoholic leaf and fruit extracts of seabuckthorn to inhibit the oxidative damage induced by chromium was investigated. Addition

  16. Evaluation of antioxidative properties of Geranium macrorrhizum and Potentilla fruticosa extracts in Dutch style fermented sausages

    Microsoft Academic Search

    G. Miliauskas; E. Mulder; J. P. H. Linssen; J. H. Houben; T. A. van Beek; P. R. Venskutonis

    2007-01-01

    Antioxidative properties of Geranium macrorrhizum, Potentilla fruticosa and Rosmarinus officinalis (as a reference) extracts were evaluated in Dutch style fermented sausages. Extracts were incorporated into sausages during preparation. The sausages were subsequently fermented, tested and compared to a standard spices mix, traditionally used for the production of such sausages. Formation of the primary oxidation products – peroxides, and secondary –

  17. Antioxidant properties of water and ethanol extracts from hot air-dried and freeze-dried daylily flowers

    Microsoft Academic Search

    Lin-Chun Mao; Xin Pan; Fei Que; Xue-Hua Fang

    2006-01-01

    Daylily (Hemerocallis fulva Linn.) flowers were hot air-dried and freeze-dried after harvest. Water and ethanol extracts were\\u000a prepared from these dried flowers and their antioxidant properties were evaluated using total antioxidant activity, reducing\\u000a capacity, metal chelating activity, and DPPH and superoxide anion radical scavenging activities, comparing with standards.\\u000a Extracts from daylily flowers exhibited strong antioxidant activity. Ethanol was more efficienct

  18. Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year

    PubMed Central

    2012-01-01

    Background There is no available information on physicochemical and antioxidant properties on Bangladeshi honey. We investigated five different monofloral and three different multifloral honey samples collected from different parts of Bangladesh. Methods The levels of phenolics, flavonoids, ascorbic acid, ascorbic acid equivalent antioxidant content (AEAC), proline, protein and antioxidants were determined in the honey samples using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Results The highest level of phenolic was 688.5?±?5.9 mg Gallic acid/kg, and the highest level of flavonoid was 155?±?6.9 mg Catechin/kg. The highest color intensity was 2034.00?±?17.5 mAU, and the highest protein content was 8.6?±?0.0mg/g. High levels of proline (2932.8?±?3.7 mg/kg), ascorbic acid (154.3?±?0.3 mg/kg), AEAC (34.1?±?1.4mg/100 g) and FRAP (772.4?±?2.5 ?mol Fe (II)/100 g) were detected in some of the samples, especially the multifloral honey samples, indicating good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH, FRAP and color intensity, indicating that in addition to total phenolic and flavonoid concentrations, color intensity and amino acid are good indicators of the antioxidant potential of honey. Except for a single sample (BDH-6), the honey samples stored for 1.5 years at room temperature still had 5-hydroxymethylfurfural (HMF) values within the recommended range (mean = 10.93 mg/kg), indicating that the rate of HMF production in Bangladeshi honey samples is low. Conclusion It is postulated that the low rate of HMF formation could be attributed to the acidic and low moisture content in the samples. In general, multifloral honeys have higher antioxidant properties based on their high levels of phenolics, flavonoids, AEAC, DPPH and FRAP when compared to monofloral honeys. We also found that monofloral honey samples from Guizotia abyssinica and Nigella sativa had high antioxidant properties. PMID:23043497

  19. A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species.

    PubMed

    Martínez-Sánchez, Ascensión; Gil-Izquierdo, Angel; Gil, María I; Ferreres, Federico

    2008-04-01

    A comparative study of antioxidant compounds, flavonoids and vitamin C, and also antioxidant activity was carried out in four species of Brassicaceae vegetables used for salads: watercress ( Nasturtium officinale R. Br.), mizuna [ Brassica rapa L. subsp. nipposinica (L.H. Bailey) Haneltand], wild rocket [ Diplotaxis tenuifolia (L.) DC.], and salad rocket [ Eruca vesicaria (L.) Cav.]. The characterization of individual phenolic compounds by HPLC-DAD-MS/MS-ESI in watercress and mizuna completes the polyphenol study previously reported for wild rocket and salad rocket. The qualitative study of flavonoids in watercress leaves showed a characteristic glycosylation pattern with rhamnose at the 7 position. Isorhamnetin 3,7-di- O-glucoside was identified in mizuna leaves and may be considered a chemotaxonomical marker in some B. rapa subspecies. Brassicaceae species showed differences in the quantitative study of flavonoids, and the highest content was detected in watercress leaves. Watercress and wild rocket leaves had the highest content of vitamin C. The antioxidant activity evaluated by different methods (ABTS, DPPH, and FRAP assays) showed a high correlation level with the content of polyphenols and vitamin C. In conclusion, the Brassicaceae leaves studied, watercress, mizuna, wild rocket, and salad rocket, presented a large variability in the composition and content of antioxidant compounds. These baby leaf species are good dietary sources of antioxidants with an important variability of bioactive compounds. PMID:18321050

  20. Potentiating effects of honey on antioxidant properties of lemon-flavoured black tea.

    PubMed

    Pereira, Carla; Barros, Lillian; Vilas-Boas, Miguel; Ferreira, Isabel C F R

    2013-03-01

    Health benefits including antioxidant potential of black tea (Camellia sinensis), lemon (Citrus limon) and honey bees (Apis mellifera) have been extensively reported. Nevertheless, nothing is reported about the effects of their concomitant use. Herein, those effects were evaluated in infusions of lemon-flavoured black tea with three different kinds of honey (light amber, amber and dark amber) from Lavandula stoechas, Erica sp. pl. and other indigenous floral species from north-east Portugal, a region with high amounts of this food product. Data obtained showed that the use of honey (dark amber>amber>light amber) potentiates the antioxidant activity of lemon-flavoured black tea, increasing the reducing power and lipid peroxidation inhibition properties, as also the antioxidant contents such as phenolics, flavonoids and organic acids including ascorbic acid. PMID:22856394

  1. The antioxidant properties of theaflavins and their gallate esters--radical scavengers or metal chelators?

    PubMed

    Miller, N J; Castelluccio, C; Tijburg, L; Rice-Evans, C

    1996-08-19

    The antioxidant properties of theaflavins and their gallate esters were studied by investigating their abilities to scavenge free radicals in the aqueous and lipophilic phases. The total relative antioxidant activities in the aqueous phase were assessed by measuring their direct ABTS.+ radical scavenging abilities, and by their efficacies in inhibiting the degradation of deoxyribose induced by iron. The propensities for enhancing the resistance of LDL to oxidation mediated by Cu2+ were also measured. The results show that the hierarchy of reactivity of these compounds as antioxidants is: theaflavin digallate > 3'-monogallate = 3-monogallate > theaflavin. Spectroscopic studies show that all the compounds chelate iron and copper; enhanced absorbance in the visible region is observed in the case of the iron-digallate complex, but not with copper. PMID:8769311

  2. Quality and antioxidant properties of breads enriched with dry onion (Allium cepa L.) skin.

    PubMed

    Gawlik-Dziki, Urszula; ?wieca, Micha?; Dziki, Dariusz; Baraniak, Barbara; Tomi?o, Justyna; Czy?, Jaros?aw

    2013-06-01

    The aim of the study was to investigate the effect on the antioxidant properties and sensory value of bread of adding ground onion skin (OS). For a determination of bioaccessibility and bioavailability in vitro the human gastrointestinal tract model was used. OS contained mastication-extractable quercetin (4.6 mg/g). Quercetin from OS was highly bioaccessible during in vitro conditions, but only approximately 4% of quercetin released during simulated digestion was bioavailable in vitro. The antioxidant potential of bread with OS was significantly higher than the activity noted in the control. In particular, OS addition significantly fortificated bread with bioaccessible lipid oxidation preventers and compounds with reducing and chelating abilities. The 2-3% OS addition caused significant improvement of antioxidant abilities (further increases in the OS supplement did not increase the activity of bread). Sensory evaluation showed that replacement of wheat flour in bread with up to 3% OS powder gave satisfactory consumer acceptability. PMID:23411290

  3. Antioxidative properties of Pandanus amaryllifolius leaf extracts in accelerated oxidation and deep frying studies.

    PubMed

    Nor, Fatihanim Mohd; Mohamed, Suhaila; Idris, Nor Aini; Ismail, Razali

    2008-09-15

    The potential uses of Pandanus amaryllifolius leaf extract as a natural antioxidant were evaluated in refined, bleached and deodorized (RBD) palm olein, using accelerated oxidation and deep frying studies at 180°C from 0 to 40h. The extracts (optimum concentration 0.2%) significantly retarded oil oxidation and deterioration (P<0.05), comparably to 0.02% BHT in tests such as peroxide value, anisidine value, iodine value, free fatty acid, oxidative stability index (OSI), polar and polymer compound contents. In sensory evaluation studies, different batches of French fries were not significantly different (P<0.05) from one another for oiliness, crispiness, taste and overall acceptability when the same oil was used for up to the 40th hour of frying. P. amaryllifolius leaf extract, which had a polyphenol content of 102mg/g, exhibited an excellent heat-stable antioxidant property and may be a good natural alternative to existing synthetic antioxidants in the food industry. PMID:26049222

  4. Development of an active food packaging system with antioxidant properties based on green tea extract.

    PubMed

    Carrizo, Daniel; Gullo, Giuseppe; Bosetti, Osvaldo; Nerín, Cristina

    2014-01-01

    A formula including green tea extract (GTE) was developed as an active food packaging material. This formula was moulded to obtain an independent component/device with antioxidant properties that could be easily coupled to industrial degassing valves for food packaging in special cases. GTE components (i.e., gallic acid, catechins and caffeine) were identified and quantified by HPLC-UV and UPLC-MS and migration/diffusion studies were carried out. Antioxidant properties of the formula alone and formula-valve were measured with static and dynamic methods. The results showed that the antioxidant capacity (scavenging of free radicals) of the new GTE formula was 40% higher than the non-active system (blank). This antioxidant activity increased in parallel with the GTE concentration. The functional properties of the industrial target valve (e.g., flexibility) were studied for different mixtures of GTE, and good results were found with 17% (w/w) of GTE. This new active formula can be an important addition for active packaging applications in the food packaging industry, with oxidative species-scavenging capacity, thus improving the safety and quality for the consumer and extending the shelf-life of the packaged food. PMID:24266419

  5. Antioxidant properties of catechins and proanthocyanidins: effect of polymerisation, galloylation and glycosylation.

    PubMed

    Plumb, G W; De Pascual-Teresa, S; Santos-Buelga, C; Cheynier, V; Williamson, G

    1998-10-01

    A range of catechins and oligomeric procyanidins was purified by high performance liquid chromatography (HPLC) from grape seed, apple skin, lentil and almond flesh. Catechins, galloylated epicatechin, glycosylated catechin, procyanidin dimers, galloylated dimers, trimer, and tetramer species were all identified, purified and quantified by HPLC, LC-MS and NMR. The antioxidant properties of these compounds were assessed using two methods: (a) inhibition of ascorbate/iron-induced peroxidation of phosphatidylcholine liposomes; (b) scavenging of the radical cation of 2,2'-azinobis(3-ethyl-benzothiazoline-6-sulphonate) (ABTS) relative to the water-soluble vitamin E analogue Trolox C (expressed as Trolox C equivalent antioxidant capacity, TEAC). Antioxidant activity in the lipid phase decreased with polymerisation in contrast with antioxidant action in the aqueous phase which increased from monomer to trimer and then decreased from trimer to tetramer. Galloylation of catechin and dimeric procyanidins decreased lipid phase and increased aqueous phase antioxidant activity. Glycosylation of catechin demonstrated decreased activity in both phases. PMID:9860050

  6. Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties

    PubMed Central

    Mandal, Shreya; Patra, Arpita; Samanta, Animesh; Roy, Suchismita; Mandal, Arpita; Mahapatra, Tapasi Das; Pradhan, Shrabani; Das, Koushik; Nandi, Dilip Kumar

    2013-01-01

    Objective To investigate phytochemical screening, antimicrobial activity and qualitative thin layer chromatographic separation of flavonoid components, antioxidant activity and total flavonoid compound of Terminalia arjuna. Methods For phytochemical screening, some common and available standard tests were done. Antimicrobial bioassay was done through agar well diffusion method. Detection of antioxidant activity and flavonoid compounds were done through thin layer chromatography. Total antioxidant activity was measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) in colorimetric method. Aluminum chloride colorimetric method was used for total flavonoid determination. Results Phytochemical screening showed the active compounds presence in high concentration, such as phytosterol, lactones, flavonoids, phenolic compounds and tannins and glycosides. The antimicrobial activity of extract showed that greater inhibition zone against Gram negative bacteria than Gram positive bacteria. This methanolic extract showed a promising antioxidant activity, as absorption of DPPH redicles decreased in DPPH free radical scavenging assay. Flavonoids components having antioxidant property present in the methanol extract at a level of 199.00 mg quercetin equivalent/g of dried methanol extract in colorimetric method. Conclusions The Terminalia arjuna bark extract revealed the presence of bio-active constituents which are known to exhibit medicinal as well as physiological activities. PMID:24093787

  7. Antioxidant and photoprotective properties of an extract from buckwheat herb (Fagopyrum esculentum MOENCH).

    PubMed

    Hinneburg, I; Kempe, S; Rüttinger, H H; Neubert, R H H

    2006-03-01

    In recent years, the incidence of skin cancer has risen remarkably. Sun light, especially the included ultraviolet (UV)-radiation, is seen as important trigger for the development of skin cancer. Thus, there is an increasing interest in the development of UV-protective substances to use them as sun care products. One approach is the topical application of herbal antioxidants. Plant-derived antioxidants are often extracts and therefore contain a complex mixture of constituents, like flavonoids and polyphenols, which contribute to the overall activity of the extract. In the present study an extract from buckwheat herb was compared to rutin, which is the main constituent of the extract, regarding their antioxidant and radical scavenging activity. Additionally, the photoprotective properties of the extract were compared to those of a commercial UV absorber. The antioxidant activity was quantified regarding the reactivity versus the 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH). The photoprotective properties of the extract were examined by the inhibition of the photosensitized lipid peroxidation of linolic acid. In the DPPH assay, the extract had significantly better antioxidant activity than pure rutin. The extract prevented more effectively the UV-induced peroxidation of linolic acid than rutin itself or the commercial UV absorber. The use of the extract from buckwheat herb seems to be more beneficial than the use of pure rutin. This can be referred to the presence of minor phenolic compounds in the extract. The results indicate that it is advisable to use antioxidants rather than only UV absorber to obtain a maximum of photo protection. PMID:16599267

  8. Bioavailability and in vivo antioxidant properties of lycopene from tomato products and their possible role in the prevention of cancer

    Microsoft Academic Search

    A. V. Rao; Sanjiv Agarwal

    1998-01-01

    Oxidative stress is recognized as one of the major contributors of increased risk of cancer. Many recent population studies have established a close link between dietary intake of tomatoes, a major source of the carotenoid antioxidant lycopene, and lowered risk of cancer. A study was conducted on 19 healthy human subjects to evaluate the uptake and in vivo antioxidant properties

  9. Hydrophobicity and antioxidant activity acting together for the beneficial health properties of nordihydroguaiaretic acid.

    PubMed

    Paracatu, Luana Chiquetto; Quinello Gomes de Faria, Carolina Maria; Zeraik, Maria Luiza; Quinello, Camila; Rennó, Camila; Palmeira, Patrícia; da Fonseca, Luiz Marcos; Ximenes, Valdecir Farias

    2015-06-10

    Nordihydroguaiaretic acid (NDGA) and rosmarinic acid (RA), phenolic compounds found in various plants and functional foods, have known antioxidant and anti-inflammatory properties. In the present study, we comparatively investigated the importance of hydrophobicity and oxidisability of NDGA and RA, regarding their antioxidant and pharmacological activities. Using a panel of cell-free antioxidant protocols, including electrochemical measurements, we demonstrated that the anti-radical capacities of RA and NDGA were similar. However, the relative capacity of NDGA as an inhibitor of NADPH oxidase (ex vivo assays) was significantly higher compared to RA. The inhibitory effect on NADPH oxidase was not related to simple scavengers of superoxide anions, as confirmed by oxygen consumption by the activated neutrophils. The higher hydrophobicity of NDGA was also a determinant for the higher efficacy of NDGA regarding the inhibition of the release of hypochlorous acid by PMA-activated neutrophil and cytokine (TNF-? and IL-10) production by Staphylococcus aureus-stimulated peripheral blood mononuclear cells. In conclusion, although there have been extensive studies about the pharmacological properties of NDGA, our study showed, for the first time, the importance not only of its antioxidant activity, but also its hydrophobicity as a crucial factor for pharmacological action. PMID:25927268

  10. Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities

    PubMed Central

    2012-01-01

    Background Withania somnifera, also known as ashwagandha, is an important herb in ayurvedic and indigenous medical systems. The present study was designed to evaluate the antioxidant and antibacterial activities of an 80% aqueous methanolic extract of W. somnifera roots (WSREt), fruits (WSFEt) and leaves (WSLEt). Methods Several assays were performed to determine the antioxidant properties of this herb including 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity, ferric reducing antioxidant power (FRAP), ferrous chelation and inhibition of ?-carotene bleaching. Results The values for DPPH, FRAP, ferrous chelation and inhibition of ? carotene bleaching for the three types of extracts ranged from 101.73-801.93 ?g/ml, 2.26-3.29 mM Fe/kg, 0.22-0.65 mg/ml and 69.87-79.67%, respectively, indicating that W. somnifera, particularly the leaves, possesses significant antioxidant properties. The mean ascorbic acid content was 20.60-62.60 mg/100 g, and the mean anthocyanin content was 2.86-12.50 mg/100 g. Antibacterial activities were measured using the agar well diffusion method and five pathogenic Gram-negative bacteria: Escherichia coli, Salmonella typhi, Citrobacter freundii, Pseudomonas aeruginosa and Klebsiella pneumoniae. The leaf extracts displayed the highest activity against S. typhi (32.00 ± 0.75 mm zone of inhibition), whereas the lowest activity was against K. pneumoniae (19.00 ± 1.48 mm zone of inhibition). The lowest minimum inhibitory concentration value was 6.25 mg/ml, which was against S. typhi, followed by 12.5 mg/ml against E. coli. Conclusion In addition to its antioxidant properties, W. somnifera exhibited significant antibacterial activities against Gram-negative bacteria, particularly S. typhi. PMID:23039061

  11. PBN derived amphiphilic spin-traps. II/Study of their antioxidant properties in biomimetic membranes.

    PubMed

    Ortial, Stéphanie; Morandat, Sandrine; Bortolato, Muriel; Roux, Bernard; Polidori, Ange; Pucci, Bernard; Durand, Grégory

    2014-01-01

    The work reported herein deals with the evaluation of the antioxidant properties of bitailed amphiphilic ?-phenyl-N-tert-butylnitrone derivatives (BPBNs) towards oxidation of an unsaturated lipid, the 1,2-dilinoleoyl-sn-glycero-3-phosphocholine (DLoPC). Oxidation was induced either by UV light irradiation or radical initiators, i.e. the water soluble AAPH and the Fenton reaction, and the antioxidant evaluation was carried out using two biomimetic systems, namely Langmuir monolayers and large unilamellar vesicles. Measurement of the molecular area and the membrane fluidity of pure nitrone monolayers before and after UV-irradiation demonstrated the better stability and antioxidant properties of B17PBN, the derivative with two C17H35 alkyl chains, compared to its analogue B11PBN with two C11H23 alkyl chains. At only 5% molar ratio of nitrone in mixed DLoPC/nitrone monolayers, a complete inhibition of the molecular area decrease was observed for B17PBN whereas B11PBN showed lower protection. The oxidation of mixed DLoPC/nitrones large unilamellar vesicles in the presence of free radicals arising from AAPH decomposition or Fenton reaction was assessed by measuring lipid conjugated dienes and thiobarbituric acid reactive substances on the whole series of nitrone, i.e. C11-, C13-, C15- and C17-based compounds. Compared to the saturated 1,2-dimyristoyl-sn-glycero-3-phosphocholine, all bitailed amphiphilic nitrones were able to decrease conjugated dienes and TBARS in both oxidative paradigms, demonstrating therefore antioxidant property. The inhibition of phospholipids oxidation was increased when increasing the concentration of nitrone with the two B11PBN and B13PBN derivatives exhibiting higher potency. This study underlines the importance in the choice of a model membrane system when evaluating the potency of antioxidants against lipid oxidation. PMID:24121081

  12. Hypolipidaemic and antioxidant properties of ethanol extract from Flos populi.

    PubMed

    Zhao, Yan; Tang, Guosheng; Cai, Enbo; Liu, Shuangli; Zhang, Lianxue; Wang, Shijie

    2014-01-01

    This study was undertaken to evaluate antihyperlipidaemic and in vitro antioxidant activity of ethanolic extract of Flos populi (EFP). The results demonstrated that EFP contains 78.5% flavonoids and 10.4% phenolics, and it exhibited free radical-scavenging activity on 2,2-diphenyl-picrylhydrazyl (IC50 36.40 ± 0.62 ?g/mL) and high reducing power (EC50 206.32 ± 1.6l ?g/mL) under in vitro chemical assays. And irrespective of prophylactic administration or remedial administration, in high fat diet-induced hyperlipidaemic mice, oral treatment with EFP produced a decrease in the levels of serum total cholesterol, triglycerides, low-density lipoprotein-cholesterol and increase in high-density lipoprotein-cholesterol. In a word, in high fat diet-fed hyperlipidaemic mice, EFP (100, 200 and 400 mg/kg) significantly altered the plasma lipid levels to near normal. These results support a potential effect of EFP in cardiovascular disease. PMID:24730759

  13. Antioxidant activity of yogurt made from milk characterized by different casein haplotypes and fortified with chestnut and sulla honeys.

    PubMed

    Perna, Annamaria; Intaglietta, Immacolata; Simonetti, Amalia; Gambacorta, Emilio

    2014-11-01

    The aim of this work was to evaluate the antioxidant activity of yogurt made from milk characterized by different casein (CN) haplotypes (?s1-, ?-, ?-CN) and fortified with chestnut and sulla honeys. The CN haplotype was determined by isoelectric focusing, whereas antioxidant activity of yogurt was measured using 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid and ferric-reducing antioxidant power. The statistical analysis showed a significant effect of the studied factors. The results showed that chestnut honey presented the highest phenolic acid and flavonoid contents, which are closely associated with its high antioxidant activity. The antioxidant activity of fortified yogurt samples was affected both by different CN haplotypes and by type of honey added. Yogurts fortified with chestnut honey showed higher antioxidant activity than those fortified with sulla honey. The different behavior observed among the fortified yogurts led us to hypothesize that the effects of protein-polyphenol complex on antioxidant activity are interactive. The results suggest that milk proteins polymorphism and polyphenols play different roles in affecting the bioavailability and the antioxidant activity of yogurt. PMID:25200788

  14. Antioxidant properties of cerium oxide nanocrystals as a function of nanocrystal diameter and surface coating.

    PubMed

    Lee, Seung Soo; Song, Wensi; Cho, Minjung; Puppala, Hema L; Nguyen, Phuc; Zhu, Huiguang; Segatori, Laura; Colvin, Vicki L

    2013-11-26

    This work examines the effect of nanocrystal diameter and surface coating on the reactivity of cerium oxide nanocrystals with H2O2 both in chemical solutions and in cells. Monodisperse nanocrystals were formed in organic solvents from the decomposition of cerium precursors, and subsequently phase transferred into water using amphiphiles as nanoparticle coatings. Quantitative analysis of the antioxidant capacity of CeO2-x using gas chromatography and a luminol test revealed that 2 mol of H2O2 reacted with every mole of cerium(III), suggesting that the reaction proceeds via a Fenton-type mechanism. Smaller diameter nanocrystals containing more cerium(III) were found to be more reactive toward H2O2. Additionally, the presence of a surface coating did not preclude the reaction between the nanocrystal surface cerium(III) and hydrogen peroxide. Taken together, the most reactive nanoparticles were the smallest (e.g., 3.8 nm diameter) with the thinnest surface coating (e.g., oleic acid). Moreover, a benchmark test of their antioxidant capacity revealed these materials were 9 times more reactive than commercial antioxidants such as Trolox. A unique feature of these antioxidant nanocrystals is that they can be applied multiple times: over weeks, cerium(IV) rich particles slowly return to their starting cerium(III) content. In nearly all cases, the particles remain colloidally stable (e.g., nonaggregated) and could be applied multiple times as antioxidants. These chemical properties were also observed in cell culture, where the materials were able to reduce oxidative stress in human dermal fibroblasts exposed to H2O2 with efficiency comparable to their solution phase reactivity. These data suggest that organic coatings on cerium oxide nanocrystals do not limit the antioxidant behavior of the nanocrystals, and that their redox cycling behavior can be preserved even when stabilized. PMID:24079896

  15. Antioxidant properties and polyphenolic compositions of fruits from different European cranberrybush (Viburnum opulus L.) genotypes.

    PubMed

    Kraujalyt?, Vilma; Venskutonis, Petras Rimantas; Pukalskas, Audrius; ?esonien?, Laima; Daubaras, Remigijus

    2013-12-15

    Antioxidant properties of fruit juices of six Viburnum opulus genotypes were evaluated by DPPH, ABTS(+) radical scavenging capacity (RSC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC) and Folin-Ciocalteu total phenolic content (TPC) assays. TPC varied in the range of 5.4-10.6 mg gallic acid equivalents/g, RSC (ABTS(+)), FRAP and ORAC values were 31.9-109.8, 32.3-61.8 and 141.6-260.4 ?mol trolox equivalents/g, respectively. V. opulus var. sargentii fruit juice was a remarkably stronger antioxidant than the other five V. opulus genotypes. The content of chlorogenic acid (the main phenolic compound in berry juices) depending on plant cultivar varied in the range of 0.54-6.93 mg/ml. The RSC of individual constituents was measured by the on-line HPLC-UV-DPPH method: chlorogenic acid was the dominant radical scavenger in V. opulus P3 (74%), while epicatechin and catechin (the main antioxidants in V. opulus var. sargentii) contributed to 40% and 23% of the total RSC for the sargentii genotype. Nine constituents were identified in V. opulus juice by using ultra high performance liquid chromatography coupled to quadruple and time-of-flight mass spectrometers (UPLC-QTOF-MS). In general, the study demonstrated that V. opulus var. sargentii followed by V. opulus P3 and V. opulus var. americanum possessed the highest antioxidant capacity. The obtained results may assist in selecting the most valuable V. opulus genotypes for the production of fruits possessing strong antioxidant capacity and containing beneficial phenolic constituents. PMID:23993538

  16. In vitro free radical scavenging and antioxidant properties of ethanol extract of Terminalia glaucescens

    PubMed Central

    Olugbami, J. Olorunjuwon; Gbadegesin, Michael A.; Odunola, Oyeronke A.

    2015-01-01

    Background: Reactive oxygen species (ROS) are implicated in various pathological conditions. Synthetic antioxidants have adverse health effects, while many medicinal plants have antioxidant components that can prevent the harmful effects of ROS. Objectives: This study quantitatively determined the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant properties of ethanol extract of the stem bark of Terminalia glaucescens (EESTG). Materials and Methods: The objectives were achieved based on in vitro assays. Data were analyzed by Sigma Plot (version 11.0). Results: Using gallic acid as the standard compound, TPC value obtained was 596.57 ?g GAE/mg extract. TFC content of EESTG, determined as quercetin equivalent was 129.58 ?g QE/mg extract. Furthermore, EESTG significantly (P < 0.001) displayed higher reducing power activity than the standard compounds (ascorbic acid and butylated hydroxytoluene [BHT]). Total antioxidant capacity assay, measured by phosphomolybdate method, was 358.33 ± 5.77 ?g butylated hydroxytoluene equivalents [BHTE]/mg extract. ?-carotene-linoleate bleaching method affirmed the potency of EESTG because of its significantly (P < 0.001) higher anti-oxidant activity when compared with quercetin and BHT. Based on DPPH assay, EESTG displayed significantly (P < 0.001) higher activity than BHT, while the hydroxyl radical scavenging activities of BHT and quercetin significantly (P < 0.001) exceeded that of the extract, although EESTG still displayed a high level of activity obtained as 83.77% in comparison to 92.80% of the standard compounds. Conclusion: Findings from this study indicate the presence of promisingly potent phytoconstituents in EESTG that have the capability to act as antioxidants and free radical scavengers. PMID:25598635

  17. Elucidation of antioxidant properties of wood bark derived saturated diarylheptanoids: a comprehensive (DFT-supported) understanding.

    PubMed

    Ponomarenko, Jevgenija; Trouillas, Patrick; Martin, Nicolas; Dizhbite, Tatyana; Krasilnikova, Jelena; Telysheva, Galina

    2014-07-01

    A series of diarylheptanoids, namely 1,7-bis-(3,4-dihydroxyphenyl)-heptan-3-one-5-O-D-xylopyranoside (oregonin), 1,7-bis-(3,4-dihydroxyphenyl)-3-hydroxyheptane-5-O-?-D-xylopyranoside and 1,7-bis-(4-hydroxyphenyl)-heptane-3-one-5-O-?-D-glucopyranoside (platyphylloside), were isolated from the bark of alder family trees, a species widely spread over in Europe. As antioxidants, these natural polyphenols have a promising potential in various fields of application, but their redox reactivity is insufficiently characterized. In this work, their antioxidant activity is described using assays based on DPPH and ABTS(+) radical scavenging, oxygen anion radicals (O2(-)) quenching. The standardized ORAC assay was also achieved, which measures the capacity to protect fluorescent molecules against oxidative degradation. The measured antioxidant activity was higher than that of the well-known antioxidant and biologically active diarylheptanoid curcumin. Molecular modeling was used to rationalize the differences in activity and the mechanisms of action. Thermodynamic descriptors mainly O-H bond dissociation enthalpies (BDEs) establish a clear structure-activity relationship. PMID:24703933

  18. Neuroprotective, antimicrobial, antioxidant, chemotherapeutic, and antidiabetic properties of Salvia Reuterana: A mini review

    PubMed Central

    Jafari, Elham; Andalib, Sasan; Abed, Alireza; Rafieian-Kopaei, Mahmoud; Vaseghi, Golnaz

    2015-01-01

    Objectives: Herbal medicine is known as a valid alternative treatment. Salvia Reuterana, which has been used in the Iranian traditional medicine, is mostly distributed in the central highlands of Iran. Salvia Reuterana is a medicinal herb with various therapeutic usages. The aim of the present review is to take account of pharmacological properties of Salvia Reuterana. Materials and Methods: The present review summarizes the literature with respect to various pharmacological properties of Salvia Reuterana. Results: Salvia Reuterana possesses neurological, antimicrobial, antioxidant, chemotherapeutic, and antidiabetic properties. Conclusions: Salvia Reuterana can be used as an alternative for treatment of several disorders. PMID:25767752

  19. Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions

    PubMed Central

    2013-01-01

    Background Diabetes is a serious metabolic disorder affecting the metabolism of carbohydrate, protein and fat. A number of studies have shown that diabetes mellitus is associated with oxidative stress, leading to an increased production of reactive oxygen species. Ficus deltoidea is traditionally used in Malaysia for regulating blood sugar, blood pressure and cholesterol levels. The use of F. deltoidea as an alternative medicinal herb is increasingly gaining popularity with the sale of F. deltoidea tea bags and capsules in the local market. The present study was undertaken to investigate the antidiabetic and antioxidant activities of the fruits from different varieties of F. deltoidea, employing in vitro methods. Method Two fruit varieties of F. deltoidea (var. angustifolia (SF) and var. kunstleri (BF)) were extracted separately using double-distilled water. The resulting aqueous extracts were partitioned using ethyl acetate to obtain the ethyl acetate and water fractions. The crude aqueous extracts and the corresponding fractions were evaluated for their phenolic, flavonoid, sugar and protein contents. Protein profiling of the extracts and fractions were also carried out by means of SDS-PAGE and SELDI-TOF MS. Antidiabetic activities were assessed based on the ability of the samples to inhibit yeast and mammalian ?-glucosidase as well as ?-amylase. Antioxidant capacities were examined by measuring the ability of the samples to reduce ferric ions and to scavenge DPPH, superoxide anion, ABTS and nitric oxide radicals. Results The crude extracts and fractions of SF and BF inhibited both yeast and rat intestinal ?-glucosidases in a dose-dependent manner, but did not inhibit porcine pancreatic ?-amylase. The water fraction of BF showed the highest percentage of ?-glucosidase inhibition while having the highest amount of protein (73.33?±?4.99 ?g/mg fraction). All the extracts and fractions exhibited antioxidant activities, with SF crude extract showing the highest antioxidant activity and phenolic content (121.62?±?4.86 mg/g extract). Fractionation of the crude extracts resulted in loss of antioxidant activities. There was no positive correlation between phenolic and flavonoid content with ?-glucosidase inhibitory activities. However, phenolic content correlated well with antioxidant activities of the crude extracts but not with the fractions. Conclusions The antioxidant activities of the fruits of F. deltoidea might be asserted by the phenolic content but other polar plant components were possibly involved in the antidiabetic properties. The study of these compounds having both antihyperglycemic and antioxidant activities may provide a new approach in the treatment of diabetes mellitus. PMID:23718315

  20. [Antioxidant properties of lactic acid bacteria--probiotic and yogurt strains].

    PubMed

    Uskova, M A; Kravchenko, L V

    2009-01-01

    Antioxidant properties of 14 strains of lactic acid bacteria were evaluated in vitro using FRAP assay, inhibition of luminol oxidation in Hb-H2O2 system and inhibition of NADPH-Fe2+ induced microsomal lipid peroxidation. All strains demonstrated high reducing properties, but only L. casei spp. (including L. casei 114001) and L. fermentum ME-3 revealed pronounced ability to suppress oxidation of luminol (by 43-65,8%) and microsomal lipid peroxidation (by 57,9-89,5%). Either L. casei 114001 (10(8) CFU suspended in physiological solution) or fermented dairy drink containing equivalent amount of L. casei 114001 were daily administered orally to male Wistar rats. Antioxidant capacity of blood plasma, liver and intestines of animals elevated while MDA content in blood plasma decreased. PMID:19514338

  1. Characterization and Properties of Nanoparticles

    NASA Astrophysics Data System (ADS)

    Baer, Donald

    2008-05-01

    Nanoparticles and other nanostructured materials are increasingly subject to a wide variety of studies. Although it is often ignored, in many circumstances the character and properties of nano-structured materials vary as a function of time, are altered by coatings and are environmentally dependent. This talk will outline some of the general issues related to characterization of nanoparticles and show measurements of the time and environmentally dependent behaviors of iron metal-core oxide-shell and ceria nanoparticles. Iron nanoparticles are of interest for several reasons including their potential use for medical applications and reduction of environmental contaminants such as chlorinated hydrocarbons or chromates. A study of the changes that occur when iron nanoparticles are exposed to water and the impact of the changes on particle reactivity and reaction pathway will be discussed. For this work, XPS, TEM, BET and XRD have been used to obtain information about the changes that take place in the particles as a function of time. Because of an ability to store and release oxygen, ceria is important for catalytic, fuel cell and possibly health applications. Other researchers have shown that as CeO2 particles decrease in size, the Ce valence state changes from a Ce^+4 to a Ce^+3. We have found that this transformation is environmentally dependent. Five nm particles can change from +4 to +3 and back to +4, as the environment is altered from oxidizing to reducing conditions and back. These changes can be observed in solution using optical adsorption and may have impacted measurements intended to examine band-gap changes due to particle size. XPS measurements of particles fully corroborate the real time solution measurements. These time and environmentally dependent measurements create important sample and characterization handling challenges.

  2. Evaluation of in-vitro Antioxidant Properties of Hydroalcoholic Solution Extracts Urtica dioica L., Malva neglecta Wallr. and Their Mixture.

    PubMed

    Güder, Aytaç; Korkmaz, Halil

    2012-01-01

    The study was aimed at evaluating the antioxidant activity of hydroalcoholic solution extracts of Urtica dioica L. (UD), Malva neglecta Wallr. (MN) plants and their mixture. In this study, flower (UDF), root (UDR), seed (UDS) and leaf (UDL) parts of UD and flower (MNF) and leaf (MNL) parts of MN were used. The antioxidant properties of hydroalcoholic extracts and their mixture were evaluated using different antioxidant tests such as total antioxidant activity, reducing power, superoxide anion radical scavenging, hydrogen peroxide scavenging, free radical scavenging, and metal chelating activity for comparison. In addition, total phenolic compounds in the extracts of both plants were determined as catechin equivalent. The various antioxidant activities were compared to natural and synthetic standard antioxidants such as BHA, BHT and ?-tocopherol. According to FTC method, the both extracts exhibited strong total antioxidant activity. At the concentration of 100 ?g/mL, Hydroalcoholic extracts of UDS, UDR, UDF, UDL, MNF, MNL, and UD-MN showed 81.7%, 79.8%, 78.3%, 76.4%, 77.3%, 74.1%, and 80.7%, respectively. Comparable, 100 ?g/mL of standard antioxidants BHA, BHT and ?-tocopherol exhibited 66.2%, 70.6%, and 50.1% inhibition on peroxidation of linoleic acid emulsion, respectively. In addition, UD-MN showed strong superoxide anion radical scavenging activity comparable with UDR, UDF, UDL, MNF, and MNL. Based on the findings, plants mixture was commonly found to have synergistically higher antioxidant activity. PMID:24250519

  3. Physicochemical and antioxidant properties of Malaysian honeys produced by Apis cerana, Apis dorsata and Apis mellifera

    PubMed Central

    2013-01-01

    Background The aim of the present study was to evaluate the physicochemical and antioxidant properties of Malaysian monofloral honey samples—acacia, pineapple and borneo honey—and compare them with tualang honey. Acacia and pineapple honey are produced by Apis mellifera bees while borneo and tualang honey are produced by Apis cerana and Apis dorsata bees, respectively. Methods The physical parameters of honey, such as pH, moisture content, electrical conductivity (EC), total dissolved solids (TDS), color intensity, total sugar and apparent sucrose content, were measured. Hydroxymethylfurfural (HMF) was measured using high performance liquid chromatography, and a number of biochemical and antioxidant tests were performed to determine the antioxidant properties of the honey samples. Results Acacia honey was the most acidic (pH 3.53), whereas pineapple honey had the lowest moisture content (14.86%), indicating that both types of honey can resist microbial spoilage more effectively when compared to tualang honey (pH 3.80 and 17.53% moisture content). Acacia honey contained the highest EC (0.76 mS/cm), whereas borneo honey had the highest (377 ppm) TDS. The mean HMF content in Malaysian honey was 35.98 mg/kg. Tualang honey, which is amber color, had the highest color intensity (544.33 mAU). Acacia honey is the sweetest, and contained the highest concentration of total sugar, reducing sugar and apparent sucrose. Tualang honey had the highest concentration of phenolic compounds (352.73 ± 0.81 mg galic acid/kg), flavonoids (65.65 ± 0.74 mg catechin/kg), DPPH (59.89%), FRAP values (576.91 ± 0.64 ?M Fe (II)/100 g) and protein content (4.83 ± 0.02 g/kg) as well as the lowest AEAC values (244.10 ± 5.24 mg/kg), indicating its strong antioxidant properties. Proline, an important amino acid that is present in honey was also measured in the present study and it was found at the highest concentration in pineapple honey. Several strong correlations were found among the biochemical and antioxidant parameters of all the Malaysian honeys. Conclusion Although Malaysian honeys are of good quality, tualang honey contains the strongest antioxidant properties by far. PMID:23433009

  4. Detection of caraway and bay leaves irradiation based on their extracts’ antioxidant properties evaluation

    Microsoft Academic Search

    Martin Polovka; Milan Suhaj

    2010-01-01

    Antioxidant properties of extracts prepared from native (non-irradiated) ground caraway (Carum carvi, L.) and bay leaves (Laurus nobilis, L.) samples, as well as from those ?-irradiated by Co60 source at doses from 5 to 30kGy were studied by EPR and UV–VIS spectroscopy, and expressed as Trolox Equivalent. Ferric reducing power, thiobarbituric acid reactive substances and total phenolic compounds content of

  5. Release property and antioxidant effectiveness of tocopherol-incorporated LDPE\\/PP blend films

    Microsoft Academic Search

    X. Zhu; D. S. Lee; K. L. Yam

    2012-01-01

    Low-density polyethylene (LDPE)\\/polypropylene (PP) blend films in various blending ratios containing 3000?mg?kg of tocopherol were manufactured by an extrusion process. Tocopherol release properties were characterised and correlated with antioxidant effectiveness in retarding the oxidation of linoleic acid contacting the films at 40°C. The conditions without tocopherol (control) and with instant tocopherol addition corresponding to the amount included in the films

  6. Comparative studies on the physicochemical and antioxidant properties of different tea extracts

    Microsoft Academic Search

    Haixia Chen; Yu Zhang; Xueming Lu; Zhishuang Qu

    Tea is one of the most popular drinks next to water. Tea polyphenol is one of the main bioactive constituents of tea with\\u000a health functions. In order to find the most bioactive tea polyphynols, polyphenol extracts from green tea, black tea and chemical\\u000a oxidation products of green tea extracts were comparatively studied on the physicochemical and antioxidant properties. Results\\u000a showed

  7. Isolation and characterization of collagen and antioxidant collagen peptides from scales of croceine croaker (Pseudosciaena crocea).

    PubMed

    Wang, Bin; Wang, Yu-Mei; Chi, Chang-Feng; Luo, Hong-Yu; Deng, Shang-Gui; Ma, Jian-Yin

    2013-11-01

    Acid soluble collagen (ASC) from scales of croceine croaker (ASC-C) was successfully isolated with the yield of 0.37% ± 0.08% (dry weight basis), and characterized as type I collagen on the basis of amino acid analysis and electrophoretic pattern. The antioxidant hydrolysate of ASC-C (ACH) was prepared through a two-stage in vitro digestion (4-h trypsin followed by 4-h pepsin), and three antioxidant peptides (ACH-P1, ACH-P2, and ACH-P3) were further isolated from ACH using ultrafiltration, gel chromatography, and RP-HPLC, and their amino acid sequences were identified as GFRGTIGLVG (ACH-P1), GPAGPAG (ACH-P2), and GFPSG (ACH-P3). ACH-P1, ACH-P2, and ACH-P3 showed good scavenging activities on hydroxyl radical (IC50 0.293, 0.240, and 0.107 mg/mL, respectively), DPPH radical (IC?? 1.271, 0.675, and 0.283 mg/mL, respectively), superoxide radical (IC?? 0.463, 0.099, and 0.151 mg/mL, respectively), and ABTS radical (IC?? 0.421, 0.309, and 0.210 mg/mL, respectively). ACH-P3 was also effectively against lipid peroxidation in the model system. The antioxidant activities of three collagen peptides were due to the presence of hydrophobic amino acid residues within the peptide sequences. The collagen peptides might be used as antioxidant for the therapy of diseases associated with oxidative stress, or reducing oxidative changes during storage. PMID:24284428

  8. Antioxidant and antimicrobial properties of Litsea elliptica Blume and Litsea resinosa Blume (Lauraceae)

    PubMed Central

    Wong, Mui-Hung; Lim, Li-Fang; Ahmad, Fasihuddin bin; Assim, Zaini bin

    2014-01-01

    Objective To investigate antioxidant and antimicrobial activities of two plant species, Litsea elliptica (L. elliptica) and Litsea resinosa (L. resinosa). Methods In vitro method-2,2-diphenyl-1-picrylhydrazyl radical scavenging assay was conducted for antioxidant activity determination while antimicrobial assay consisted of agar well diffusion assay and mycelial radial growth assay. Results Methanol extracts of root and stem of L. elliptica and L. resinosa exhibited the highest antioxidant activity with EC50 of 23.99, 41.69, 11.22 and 35.48 mg/L respectively. All methanol extracts of L. resinosa as well as root extracts from L. elliptica showed significant scavenging activity. Hexane extract from stem of L. resinosa presented the largest inhibition zone in Gram-negative bacteria Pseudomonas aeruginosa and Escherichia coli while chloroform extract from inner bark of L. resinosa showed major inhibition towards Gram-positive bacteria Bacillus subtilis. Essential oils from the root of both species showed significant antifungal activities which are 80.11% and 66.85% respectively. Conclusions Overall, methanol extracts from root and stem of both species showed antioxidant activity comparable to standard butylated hydroxytoluene. Extracts from L. resinosa demonstrated stronger antimicrobial properties compared to that from L. elliptica. PMID:25182724

  9. Preliminary assessment of phytochemical contents and antioxidant properties of Pistacia integerrima fruit.

    PubMed

    Abbasi, Arshad Mehmood; Guo, Xinbo; Nazir, Abdul

    2015-07-01

    Present study was focused on the evaluation of preliminary phytochemical screening and antioxidant properties in hydrophilic and lipophilic solvents extracts of Pistacia integerrima fruit, collected from Lesser Himalayas-Pakistan. Ethnobotanical data were collected through semi-structured interviews. Standard analytical methods were followed to estimate the proximate composition of nutrients, trace metals and phytochemical contents including phenolics, flavonoids, flavonols and ascorbic acid. The free radical scavenging activities of aqueous and acetone extracts were carried out against 2, 2-diphenyl-1-picrylhydrazyl, hydroxyl radical, hydrogen peroxide radical, ferrous ion chelation, ferric ion reduction, and phosphomolybdenium complex assay. Comparatively highest value of cultural importance index was determined for Margalla hills than other study sites. Crude fibers contents (33.65%) were found highest among nutrients, while in minerals utmost level was measured for K (947.3mg/kg, FW), followed by Ca, Mg, Na and Fe. Acetone extract exhibited highest total phenolics contents (113.7mg GAE/100g, FW) and antioxidant potential for ferric ion reduction (107.3?M GAE/100g, FW), phosphomolybdenum complex assay (99.32?M AAE/100g, FW) and DPPH radical scavenging (91.89%). Fruit of P. integerrima was found an excellent source of nutrients, minerals and antioxidants. Crude extracts exhibited noteworthy potential against free radicals and could be of immense significance in the prevention of different diseases related to free radicals. Present data could be an effectual tool for propagation programs intended to enhance the antioxidant and other phytochemical components in foods. PMID:26142500

  10. Synthesis, characterization and investigation of antioxidant activity of cobalt quercetin complex

    NASA Astrophysics Data System (ADS)

    Birjees Bukhari, S.; Memon, Shahabuddin; Mahroof Tahir, M.; Bhanger, M. I.

    2008-12-01

    This article describes a novel synthesis of cobalt and quercetin·2H 2O complex in methanol, characterized by using elemental analysis, UV-visible, 1H NMR, TGA, DSC and IR spectrometric techniques. The formation of complex is deduced from the UV-visible spectra which shows that the successive formation of cobalt-quercetin complex occurs in a ratio of 2:1 (metal/ligand) stoichiometrically. The antioxidant activity of the complex was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. In this work, we have shown that the metal complexed flavonoids are much more effective free radical scavengers than the free flavonoids.

  11. Antioxidant properties of modified rutin esters by DPPH, reducing power, iron chelation and human low density lipoprotein assays

    Microsoft Academic Search

    Bena-Marie Lue; Nina Skall Nielsen; Charlotte Jacobsen; Lars Hellgren; Zheng Guo; Xuebing Xu

    2010-01-01

    Practical limitations exist regarding the effectiveness of flavonoids as antioxidants in many food systems, possibly due to their poor solubility and miscibility in lipidic environments. Current strategies to improve these properties include enzymatically acylating flavonoids with lipophilic moieties. Herein, two derivatives of rutin (possessing C12:0 or C16:0 acyl groups) were assessed for their antioxidant properties, and compared with their parent

  12. Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties.

    PubMed

    Brown, J E; Khodr, H; Hider, R C; Rice-Evans, C A

    1998-03-15

    The flavonoids constitute a large group of polyphenolic phytochemicals with antioxidant properties in vitro. The interactions of four structurally related flavonoids (quercetin, kaempferol, rutin and luteolin) with Cu2+ ions were investigated in terms of the extent to which they undergo complex formation through chelation or modification through oxidation, as well as in their structural dependence. The ortho 3',4'-dihydroxy substitution in the B ring is shown to be important for Cu2+-chelate formation, thereby influencing the antioxidant activity. The presence of a 3-hydroxy group in the flavonoid structure enhances the oxidation of quercetin and kaempferol, whereas luteolin and rutin, each lacking the 3-hydroxy group, do not oxidize as readily in the presence of Cu2+ ions. The results also demonstrate that the reactivities of the flavonoids in protecting low-density lipoprotein (LDL) against Cu2+ ion-induced oxidation are dependent on their structural properties in terms of the response of the particular flavonoid to Cu2+ ions, whether chelation or oxidation, their partitioning abilities between the aqueous compartment and the lipophilic environment within the LDL particle, and their hydrogen-donating antioxidant properties. PMID:9494082

  13. Antioxidant and Hepatoprotective Properties of Tofu (Curdle Soymilk) against Acetaminophen-Induced Liver Damage in Rats

    PubMed Central

    Yakubu, Ndatsu; Oboh, Ganiyu; Olalekan, Amuzat Aliyu

    2013-01-01

    The antioxidant and hepatoprotective properties of tofu using acetaminophen to induce liver damage in albino rats were evaluated. Tofus were prepared using calcium chloride, alum, and steep water as coagulants. The polyphenols of tofu were extracted and their antioxidant properties were determined. The weight gain and feed intake of the rats were measured. The analysis of serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities and the concentrations of albumin, total protein, cholesterol, and bilirubin were analyzed. The result reveals that the antioxidant property of both soluble and bound polyphenolic extracts was significantly higher in all tofus, but the steep water coagulated tofu was recorded higher. Rats fed with various tofus and acetaminophen had their serum ALP, ALT, AST, and LDH activities; total cholesterol; and bilirubin levels significantly (P < 0.05) reduced, and total protein and albumin concentrations increased when compared with basal diet and acetaminophen administered group. Therefore, all tofus curdled with various coagulants could be used to prevent liver damage caused by oxidative stress. PMID:23533782

  14. Structural Characterization and In Vitro Antioxidant Activity of Kojic Dipalmitate Loaded W/O/W Multiple Emulsions Intended for Skin Disorders

    PubMed Central

    Marcussi, Diana Gleide; Calixto, Giovana Maria Fioramonti; Corrêa, Marcos Antonio

    2015-01-01

    Multiple emulsions (MEs) are intensively being studied for drug delivery due to their ability to load and increase the bioavailability of active lipophilic antioxidant, such as kojic dipalmitate (KDP). The aim of this study was to structurally characterize developed MEs by determining the average droplet size (Dnm) and zeta potential (ZP), performing macroscopic and microscopic analysis and analyzing their rheological behavior and in vitro bioadhesion. Furthermore, the in vitro safety profile and antioxidant activity of KDP-loaded MEs were evaluated. The developed MEs showed a Dnm of approximately 1 micrometer and a ZP of ?13?mV, and no change was observed in Dnm or ZP of the system with the addition of KDP. KDP-unloaded MEs exhibited ‘‘shear thinning” flow behavior whereas KDP-loaded MEs exhibited Newtonian behavior, which are both characteristic of antithixotropic materials. MEs have bioadhesion properties that were not influenced by the incorporation of KDP. The results showed that the incorporation of KDP into MEs improved the safety profile of the drug. The in vitro antioxidant activity assay suggested that MEs presented a higher capacity for maintaining the antioxidant activity of KDP. ME-based systems may be a promising platform for the topical application of KDP in the treatment of skin disorders. PMID:25785265

  15. Antioxidant properties of rice bran oil-based extraction products

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rice bran oil has many properties that make it an attractive functional food ingredient. Compared with other oils, rice bran oil is relatively rich in tocopherols, tocotrienols, and y-oryzanol, all of which have been shown to have numerous desirable health effects. The same compounds thought to co...

  16. Antioxidative properties of the gastrointestinal phytopharmaceutical remedy STW 5 (Iberogast ®)

    Microsoft Academic Search

    I. Germann; D. Hagelauer; O. Kelber; B. Vinson; S. Laufer; D. Weiser; H. Heinle

    2006-01-01

    Since inflammation is a common mechanism of many gastrointestinal diseases, reactive oxygen metabolites may play an important role in their pathophysiology. Therefore it is interesting to know, whether phytopharmaceuticals known to modulate gastroinstinal motor function reveal also antioxdiative properties. We tested STW 5 (Iberogast®), its constituent nine different plant extracts, and some isolated compounds which are present in STW 5

  17. DNA profiling, telomere analysis and antioxidant properties as tools for monitoring ex situ seed longevity

    PubMed Central

    Donà, M.; Balestrazzi, A.; Mondoni, A.; Rossi, G.; Ventura, L.; Buttafava, A.; Macovei, A.; Sabatini, M. E.; Valassi, A.; Carbonera, D.

    2013-01-01

    Background and Aims The germination test currently represents the most used method to assess seed viability in germplasm banks, despite the difficulties caused by the occurrence of seed dormancy. Furthermore, seed longevity can vary considerably across species and populations from different environments, and studies related to the eco-physiological processes underlying such variations are still limited in their depth. The aim of the present work was the identification of reliable molecular markers that might help in monitoring seed deterioration. Methods Dry seeds were subjected to artificial ageing and collected at different time points for molecular/biochemical analyses. DNA damage was measured using the RAPD (random amplified polymorphic DNA) approach while the seed antioxidant profile was obtained using both the DPPH (1,1-diphenyl, 2-picrylhydrazyl) assay and the Folin–Ciocalteu reagent method. Electron paramagnetic resonance (EPR) provided profiles of free radicals. Quantitative real-time polymerase chain reaction (QRT-PCR) was used to assess the expression profiles of the antioxidant genes MT2 (type 2 metallothionein) and SOD (superoxide dismutase). A modified QRT-PCR protocol was used to determine telomere length. Key Results The RAPD profiles highlighted different capacities of the two Silene species to overcome DNA damage induced by artificial ageing. The antioxidant profiles of dry and rehydrated seeds revealed that the high-altitude taxon Silene acaulis was characterized by a lower antioxidant specific activity. Significant upregulation of the MT2 and SOD genes was observed only in the rehydrated seeds of the low-altitude species. Rehydration resulted in telomere lengthening in both Silene species. Conclusions Different seed viability markers have been selected for plant species showing inherent variation of seed longevity. RAPD analysis, quantification of redox activity of non-enzymatic antioxidant compounds and gene expression profiling provide deeper insights to study seed viability during storage. Telomere lengthening is a promising tool to discriminate between short- and long-lived species. PMID:23532044

  18. Antioxidant Properties of the Extracts of Talinum Triangulare and its Effect on Antioxidant enzymes in Tissue Homogenate of Swiss Albino Rat

    PubMed Central

    Afolabi, Olakunle Bamikole; Oloyede, Omotade Ibidun

    2014-01-01

    Objectives: This study was designed to put into consideration both the in vitro and in vivo investigations on Talinum triangulare (Tt), an herbaceous perennial plant that is a native of tropical America and one of the most important vegetables in Nigeria. Methods: Total phenolic contents in (mg GAE/100 g), flavonoid contents, the ferric reducing antioxidant properties (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl free radical scavenging ability (OH-) and iron chelating ability were carried out in vivo using standard described methods while GSH, GPx, catalase and SOD were determined in vivo using standard described methods. Results: In the three different solvents extraction of T. triangulare that were studied in vitro, it was noted that ethyl acetate and ethanolic fractions of T. triangulare showed potent antioxidant activity against DPPH and iron chelating property with high phenolic content except Hydroxyl free radical scavenging ability that showed highest value in the aqueous extract, while the Reduced GSH indicated the highest in the parameter determined in vivo. Conclusion: The antioxidant properties showed in this solvent extractable component probably could have been the basis for the enhanced activities of antioxidant enzymes at very lower dose in the examined tissue homogenates. Therefore, T. triangulare can thereby serve as a means of Preventing some of major degenerative diseases challenging Humans PMID:25948971

  19. Antioxidant and Anti-Inflammatory Properties of an Aqueous Cyanophyta Extract Derived from Arthrospira Platensis: Contribution to Bioactivities by the Non-Phycocyanin Aqueous Fraction

    PubMed Central

    Jensen, Gitte S.; Attridge, Victoria L.; Beaman, Joni L.; Guthrie, Jesse; Ehmann, Axel; Benson, Kathleen F.

    2015-01-01

    Abstract The goal for this work was to characterize basic biological properties of a novel Arthrospira platensis-based aqueous cyanophyta extract (ACE), enriched in the known anti-inflammatory cyclooxygenase-2 (COX-2) inhibitor phycocyanin (PC), but also containing a high level of non-PC bioactive compounds. Antioxidant properties were tested in parallel in the Folin–Ciocalteu assay (chemical antioxidant capacity) and in the cellular antioxidant protection (CAP-e) bioassay, where both the PC and the non-PC fractions contributed to the antioxidant capacity and CAP of ACE. In contrast to the COX-2 inhibition seen in the presence of PC, the inhibition of enzymatic activity of the inflammatory mediator Lipoxygenase was associated specifically with the non-PC fraction of ACE. Inhibition of formation of reactive oxygen species (ROS) was evaluated using polymorphonuclear cells from healthy human donors. The inhibition of ROS formation was seen for both the PC and non-PC fractions, with ACE showing the most robust effect. The effects of PC, non-PC, and ACE on clotting and clot lysing was tested using a modified Euglobulin fibrinolytic assay in vitro. In the presence of PC, non-PC, and ACE, the time for clot formation and lysis was not affected; however, the clots were significantly more robust. This effect was statistically significant (p<.05) at doses between 125–500 ?g/mL, and returned to baseline at lower doses. Both PC and the non-PC fraction contributed to the antioxidant properties and anti-inflammatory effects, without a negative impact on blood clotting in vitro. This suggests a potential benefit for the consumable ACE extract in assisting the reduction of inflammatory conditions. PMID:25764268

  20. Antioxidant and anti-inflammatory properties of an aqueous cyanophyta extract derived from Arthrospira platensis: contribution to bioactivities by the non-phycocyanin aqueous fraction.

    PubMed

    Jensen, Gitte S; Attridge, Victoria L; Beaman, Joni L; Guthrie, Jesse; Ehmann, Axel; Benson, Kathleen F

    2015-05-01

    The goal for this work was to characterize basic biological properties of a novel Arthrospira platensis-based aqueous cyanophyta extract (ACE), enriched in the known anti-inflammatory cyclooxygenase-2 (COX-2) inhibitor phycocyanin (PC), but also containing a high level of non-PC bioactive compounds. Antioxidant properties were tested in parallel in the Folin-Ciocalteu assay (chemical antioxidant capacity) and in the cellular antioxidant protection (CAP-e) bioassay, where both the PC and the non-PC fractions contributed to the antioxidant capacity and CAP of ACE. In contrast to the COX-2 inhibition seen in the presence of PC, the inhibition of enzymatic activity of the inflammatory mediator Lipoxygenase was associated specifically with the non-PC fraction of ACE. Inhibition of formation of reactive oxygen species (ROS) was evaluated using polymorphonuclear cells from healthy human donors. The inhibition of ROS formation was seen for both the PC and non-PC fractions, with ACE showing the most robust effect. The effects of PC, non-PC, and ACE on clotting and clot lysing was tested using a modified Euglobulin fibrinolytic assay in vitro. In the presence of PC, non-PC, and ACE, the time for clot formation and lysis was not affected; however, the clots were significantly more robust. This effect was statistically significant (p<.05) at doses between 125-500 ?g/mL, and returned to baseline at lower doses. Both PC and the non-PC fraction contributed to the antioxidant properties and anti-inflammatory effects, without a negative impact on blood clotting in vitro. This suggests a potential benefit for the consumable ACE extract in assisting the reduction of inflammatory conditions. PMID:25764268

  1. Characterization of total antioxidant capacity and (poly)phenolic compounds of differently pigmented rice varieties and their changes during domestic cooking.

    PubMed

    Zaupa, Maria; Calani, Luca; Del Rio, Daniele; Brighenti, Furio; Pellegrini, Nicoletta

    2015-11-15

    In the recent years, the pigmented rice varieties are becoming more popular due to their antioxidant properties and phenolic content. In this study, we characterized the antioxidant capacity (TAC) and the phenolic profile in white, red and black rice varieties, and evaluated the effect of two cooking methods (i.e. "risotto" and boiling) on these compounds. Before the cooking, all the varieties contained several phenolic acids, whereas anthocyanins and flavonols were peculiar of black rice and flavan-3-ols of red rice. Among the rice varieties, the black had the highest TAC value. The content of (poly)phenolic compounds and TAC decreased after cooking in all three varieties, but to a lesser extent after the risotto method. As a consequence, the risotto cooking, which allows a complete absorption of water, would be a good cooking method to retain (poly)phenolic compounds and TAC in pigmented and non-pigmented whole-meal rice. PMID:25977035

  2. Preliminary study of the antioxidant properties of flowers and roots of Pyrostegia venusta (Ker Gawl) Miers

    PubMed Central

    2011-01-01

    Background Free radical stress leads to tissue injury and can eventually to arthritis, atherosclerosis, diabetes mellitus, neurodegenerative diseases and carcinogenesis. Several studies are ongoing worldwide to find natural antioxidants of plant origin. We assessed the in-vitro antioxidant activities and screened the phytochemical constituents of methanolic extracts of Pyrostegia venusta (Ker Gawl) Miers. Methods We evaluated the antioxidant potential and phytochemical constituents of P. venusta using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays. Gas chromatography-mass spectroscopy (GC-MS) studies were also undertaken to assess the phytochemical composition of the flower extracts. Results Phytochemical analyses revealed the presence of terpenoids, alkaloids, tannins, steroids, and saponins. The reducing ability of both extracts was in the range (in ?m Fe(II)/g) of 112.49-3046.98 compared with butylated hydroxytoluene (BHT; 63.56 ± 2.62), catechin (972.02 ± 0.72 ?m) and quercetin 3208.27 ± 31.29. A significant inhibitory effect of extracts of flowers (IC50 = 0.018 ± 0.69 mg/ml) and roots (IC50 = 0.026 ± 0.94 mg/ml) on ABTS free radicals was detected. The antioxidant activity of the extracts of flowers (95%) and roots (94%) on DPPH radicals was comparable with that of ascorbic acid (98.9%) and BHT (97.6%). GC-MS study revealed the presence of myoinositol, hexadecanoic acid, linoleic acid, palmitic acid and oleic acid in the flower extracts. Conclusion These data suggest that P. venusta is a natural source of antioxidants. The extracts of flowers and roots of P. venusta contain significant amounts of phytochemicals with antioxidative properties and could serve as inhibitors or scavengers of free radicals. P. venusta could be exploited as a potential source for plant-based pharmaceutical products. These results could form a sound basis for further investigation in the potential discovery of new natural bioactive compounds. PMID:21861910

  3. Physicochemical Characterization and Antioxidant Activity of Essential Oils of Guggul (Commiphora wightii) Collected from Madhya Pradesh

    PubMed Central

    Siddiqui, M. Z.; Thomas, M.; Prasad, N.

    2013-01-01

    The present study reports physicochemical characterization and antioxidant activity of essential oils extracted from guggul (Commiphora wightii) exudates collected from different places in Madhya Pradesh, India. The guggul exudates were hydrodistilled for 3-4 h in Clevenger apparatus. The oil obtained was dried over anhydrous Na2SO4 and stored at 4° until testing. Before extraction of oils from the exudates, their % moisture and tristimulus values of the colors namely L (white-black), a (green-red) and b (blue-yellow) were determined. Physicochemical characterization of the extracted oils was carried out to determine their solubility, yield%, acid value (mg/KOH/g), saponification value (mg/KOH/g), ester value, iodine value (g/g), peroxide value (mEq/kg) and Fourier transformed infrared analyses. The storage-effect on the % moisture and tristimulus values of the colors of guggul exudates as also the % oil yield and physicochemical parameters of the essential oils extracted from them, were studied using three different packaging materials viz., local plastic, low density polyethylene (200 G) and high density polyethylene (200 G). The antioxidant potential of extracted oils was evaluated by free radical scavenging activity using 1,1-diphenyl-2-picryl hydrazyl assay. PMID:24082356

  4. Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract.

    PubMed

    Jung, J E; Yoon, H J; Yu, H S; Lee, N-K; Jee, H-S; Paik, H-D

    2015-01-01

    This study investigated the effect of red ginseng extract (RGE) on the physicochemical properties, sensory test, and antioxidant activity of milk. The milk samples with RGE added at 0.5, 1, 1.5, and 2% were analyzed during storage at 4°C. The physicochemical properties included composition of milk, pH, titratable acidity, and color. The antioxidant activity of milk samples was determined using the 2,2-diphenyl-1-picrylhydrazyl method, ?-carotene bleaching assay, and ferric thiocyanate assay. An increase in the amount of RGE in milk resulted in an increase of lactose and total solids content, titratable acidity, and a* and b* values, whereas fat and protein contents remained unchanged. Also, pH and L* value decreased. The antioxidant activity of milk samples supplemented with RGE was higher than that of the control sample. Sensory evaluation was performed using a quantitative descriptive analysis. Two types of samples were used: (1) sterilized milk fortified with RGE (0.5, 1, 1.5, and 2%) and (2) 2% RGE, 2% RGE with oligosaccharide, and 2% RGE with oligosaccharide and cyclodextrin. The addition of oligosaccharide and cyclodextrin could effect an increase of sweetness, a decrease of bitterness and flavor of RGE, and aftertaste. Therefore, milk supplemented with RGE could be useful as a functional food. PMID:25465553

  5. Melatonin and male reproductive health: relevance of darkness and antioxidant properties.

    PubMed

    Rocha, C S; Rato, L; Martins, A D; Alves, M G; Oliveira, P F

    2015-01-01

    The pineal hormone melatonin controls several physiological functions that reach far beyond the regulation of the circadian rhythm. Moreover, it can be produced in extra-pineal organs such as reproductive organs. The role of melatonin in the mammalian seasonal and circadian rhythm is well known. Nevertheless, its overall effect in male reproductive physiology remains largely unknown. Melatonin is a very powerful endogenous antioxidant that can also be exogenously taken safely. Interestingly, its antioxidant properties have been consistently reported to improve the male reproductive dysfunctions associated with pathological conditions and also with the exposure to toxicants. Nevertheless, the exact molecular mechanisms by which melatonin exerts its action in the male reproductive system remain a matter of debate. Herein, we propose to present an up-to-date overview of the melatonin effects in the male reproductive health and debate future directions to disclose possible sites of melatonin action in male reproductive system. We will discuss not only the role of melatonin during darkness and sleep but also the importance of the antioxidant properties of this hormone to male fertility. Since melatonin readily crosses the physiological barriers, such as the blood-testis barrier, and has a very low toxicity, it appears as an excellent candidate in the prevention and/or treatment of the multiple male reproductive dysfunctions associated with various pathologies. PMID:25941822

  6. Physical Properties, Antioxidant and Antimicrobial Activity of Chitosan Films Containing Carvacrol and Pomegranate Peel Extract.

    PubMed

    Yuan, Gaofeng; Lv, Hua; Yang, Bingjie; Chen, Xiaoe; Sun, Haiyan

    2015-01-01

    Chitosan-based active films were developed by incorporation of carvacrol (10 g/L), pomegranate peel extract (PPE, 10 g/L) and carvacrol + PPE (10 g/L of each) and their physical, antioxidant and antimicrobial properties were investigated. Incorporation of carvacrol and carvacrol + PPE into the films significantly decreased the water vapor permeability, tensile strength and percentage of elongation at break. Incorporation of carvacrol, PPE and carvacrol + PPE into the films decreased the transparency, but significantly increased the total phenol content and antioxidant activity. All the films, with the exception of PPE-incorporated film, exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. In addition, the antibacterial activity against Staphylococcus aureus of the film incorporated with carvacrol + PPE was moderately higher than that incorporated with carvacrol or PPE alone, suggesting a synergistic action between carvacrol and PPE. PMID:26083037

  7. Antioxidant Property of Jobelyn as the Possible Mechanism Underlying its Anti-amnesic Activity in Rodents

    PubMed Central

    Umukoro, Solomon; Ugbomah, Adaeze; Aderibigbe, Adegbuyi; Omogbiya, Adrian

    2013-01-01

    Introduction Amnesia or loss of memory is the cardinal hallmark of Alzheimer's disease (AD), a progressive neurodegenerative disorder associated with ageing process. Although, AD had been discovered over a century ago, drugs which could cure or halt the progression of the disease are yet to see the light of the day. However, there has been a growing interest in the use of phytomedicines with multipronged mechanisms of action that could target various aspects of the pathologies of AD. Jobelyn (JB) is a potent antioxidant African polyherbal formulation with active components that have been acclaimed to show neuroprotection. This investigation was carried out to evaluate whether JB has anti-amnesic and antioxidant activities. Methods The alteration of alternation behavior in the Y-maze paradigm was utilized as the test for memory function in mice. The effect of JB on acetylcholinesterase (AChE) activity, malondialdehyde (MDA) level and the concentrations of glutathione (GSH) in the frontal cortex and hippocampus were assessed in rats as means of providing insight into the mechanism underlying its anti-amnesic activity. The animals were given JB (1, 2.5 or 5mg/kg, i.p.) daily for 7 days before the biochemical assays or test for memory functions were carried out. Results JB was found to produce a significant increase in the level of alternation behavior compared with the control, suggesting anti-amnesic activity. Also, JB reversed the memory impairment induced by scopolamine, which further indicates anti-amnesic property. Furthermore, JB demonstrated a significant inhibition of MDA formation in the frontal cortex and hippocampus of rats, indicating antioxidant property. In addition, it increased the defense armory of the brain tissues, as it significantly increased the concentrations of GSH in the frontal cortex and hippocampus of rats. However, JB did not demonstrate any inhibitory effect against AChE activity in the frontal cortex and hippocampus of rats in comparison with the control group. Discussion This investigation provides evidence that suggests that JB has anti-amnesic and antioxidant properties. Although the present data suggest that the anti-amnesic property of JB might be related to its antioxidant activity, more studies are necessary to clarify this observation. PMID:25337327

  8. Characterization of phytochemicals and antioxidant activities of red radish brines during lactic acid fermentation.

    PubMed

    Jing, Pu; Song, Li-Hua; Shen, Shan-Qi; Zhao, Shu-Juan; Pang, Jie; Qian, Bing-Jun

    2014-01-01

    Red radish (Raphanus L.) pickles are popular appetizers or spices in Asian-style cuisine. However, tons of radish brines are generated as wastes from industrial radish pickle production. In this study, we evaluated the dynamic changes in colour properties, phenolics, anthocyanin profiles, phenolic acid composition, flavonoids, and antioxidant properties in radish brines during lactic acid fermentation. The results showed that five flavonoids detected were four anthocyanins and one kaempferol derivative, including pelargonidin-3-digluoside-5-glucoside derivatives acylated with p-coumaric acid, ferulic acid, p-coumaric and manolic acids, or ferulic and malonic acids. Amounts ranged from 15.5-19.3 µg/mL in total monomeric anthocyanins, and kaempferol-3,7-diglycoside (15-30 µg/mL). 4-Hydroxy-benzoic, gentisic, vanillic, syringic, p-coumaric, ferulic, sinapic and salicylic acids were detected in amounts that varied from 70.2-92.2 µg/mL, whereas the total phenolic content was 206-220 µg/mL. The change in colour of the brine was associated with the accumulation of lactic acid and anthocyanins. The ORAC and Fe2+ chelation capacity of radish brines generally decreased, whereas the reducing power measured as FRAP values was increased during the fermentation from day 5 to day 14. This study provided information on the phytochemicals and the antioxidative activities of red radish fermentation waste that might lead to further utilization as nutraceuticals or natural colorants. PMID:25004074

  9. Antioxidant and DNA damage protective properties of anthocyanin-rich extracts from Hibiscus and Ocimum: a comparative study.

    PubMed

    Sarkar, Biswatrish; Kumar, Dhananjay; Sasmal, Dinakar; Mukhopadhyay, Kunal

    2014-01-01

    Anthocyanin extracts (AEs) from Ocimum tenuiflorum (leaf), Hibiscus rosa-sinensis (petal) and Hibiscus sabdariffa (calyx) were investigated and compared for in vitro antioxidant activity and DNA damage protective property. Total phenolic content (TPC) and total anthocyanin content (TAC) of the AEs were determined and the major anthocyanins were characterised. In vitro antioxidant activities were assessed by ferric-reducing antioxidant power (FRAP) assay, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical-scavenging activity, 2-deoxy-D-ribose degradation assay and lipid peroxidation assay. The protective property of the AEs was also examined against oxidative DNA damage by H2O2 and UV using pUC19 plasmid. All the AEs particularly those from O. tenuiflorum demonstrated efficient antioxidant activity and protected DNA from damage. Strong correlation between antioxidant capacity and TPC and TAC was observed. Significant correlation between antioxidant capacity and TPC and TAC ascertained that phenolics and anthocyanins were the major contributors of antioxidant activity. PMID:24730725

  10. Nutraceutical value of black cherry Prunus serotina Ehrh. fruits: antioxidant and antihypertensive properties.

    PubMed

    Luna-Vázquez, Francisco J; Ibarra-Alvarado, César; Rojas-Molina, Alejandra; Rojas-Molina, Juana I; Yahia, Elhadi M; Rivera-Pastrana, Dulce M; Rojas-Molina, Adriana; Zavala-Sánchez, Miguel Ángel

    2013-01-01

    In Mexico black cherry (Prunus serotina Ehrh.) fruits are consumed fresh, dried or prepared in jam. Considering the evidence that has linked intake of fruits and vegetables rich in polyphenols to cardiovascular risk reduction, the aim of this study was to characterize the phenolic profile of black cherry fruits and to determine their antioxidant, vasorelaxant and antihypertensive effects. The proximate composition and mineral contents of these fruits were also assessed. Black cherry fruits possess a high content of phenolic compounds and display a significant antioxidant capacity. High-performance liquid chromatography/mass spectrometric analysis indicated that hyperoside, anthocyanins and chlorogenic acid were the main phenolic compounds found in these fruits. The black cherry aqueous extract elicited a concentration-dependent relaxation of aortic rings and induced a significant reduction on systolic blood pressure in L-NAME induced hypertensive rats after four weeks of treatment. Proximate analysis showed that black cherry fruits have high sugar, protein, and potassium contents. The results derived from this study indicate that black cherry fruits contain phenolic compounds which elicit significant antioxidant and antihypertensive effects. These findings suggest that these fruits might be considered as functional foods useful for the prevention and treatment of cardiovascular diseases. PMID:24287993

  11. Antioxidant Properties of Fruits and Vegetables Shots and Juices: An Electron Paramagnetic Resonance Study

    Microsoft Academic Search

    Evaggelia D. Tzika; Vassiliki Papadimitriou; Theodore G. Sotiroudis; Aristotelis Xenakis

    2008-01-01

    The antioxidant activity of some commercially available fruit and vegetable juices was evaluated with regard to their radical\\u000a scavenging activity against the stable free radical 4-hydroxy-2,2,6,6-tetramethyl-l-piperidinyloxy (TEMPOL) monitored by electron\\u000a paramagnetic resonance (EPR) spectroscopy. TEMPOL is a stable nitroxide free radical characterized by a well-defined EPR spectrum\\u000a consisting of three peaks. The integral intensity of the EPR spectra of TEMPOL

  12. Antioxidant Properties of Amaranthus hypochondriacus Seeds and their Effect on the Liver of Alcohol-Treated Rats

    Microsoft Academic Search

    Viviana Romina Lucero López; Gabriela Silvina Razzeto; María Sofía Giménez; Nora Lilian Escudero

    Amaranth constitutes a valuable pseudocereal, due to its nutritional quality and its nutraceutical properties, which contribute\\u000a to improve human health. This work evaluated the effect of a diet based on Amaranthus hypochondriacus (Ah) seed on oxidative stress and antioxidant status in the liver of rats sub-chronically exposed to ethanol. The seed extract\\u000a was investigated for antioxidant capacity in vitro, showing

  13. Purification and characterization of flavonoids from the leaves of Zanthoxylum bungeanum and correlation between their structure and antioxidant activity.

    PubMed

    Zhang, Yujuan; Wang, Dongmei; Yang, Lina; Zhou, Dan; Zhang, Jingfang

    2014-01-01

    Nine flavonoids were isolated and characterized from the leaves of Zanthoxylum bungeanum. Their structures were elucidated by spectroscopic techniques as quercetin (1), afzelin (2), quercitrin (3), trifolin (4), quercetin-3-O-?-D-glucoside (5), isorhamnetin 3-O-?-L-rhamnoside (6), hyperoside (7), vitexin (8) and rutin (9). All compounds were isolated from the leaves of Z. bungeanum for the first time. Five compounds (2, 4, 5, 6 and 8) were found for the first time in the genus Zanthoxylum. To learn the mechanisms underlying its health benefits, in vitro (DPPH, ABTS, FRAP and lipid peroxidation inhibition assays) and in vivo (protective effect on Escherichia coli under peroxide stress) antioxidant activities of the nine flavonoids were measured. Quercetin and quercetin glycosides (compounds 1, 3, 5, 7, 9) showed the highest antioxidant activity. Structure-activity relationships indicated that the -OH in 4' position on the B ring and the -OH in 7 position on the A ring possessed high antioxidant activity; B ring and/or A ring with adjacent -OH groups could greatly increase their antioxidant ability. Also, due to the different structures of various flavonoids, they will certainly exhibit different antioxidant capacity when the reactions occur in solution or in oil-in-water emulsion. These findings suggest that Z. bungeanum leaves may have health benefits when consumed. It could become a useful supplement for pharmaceutical products and functional food ingredients in both nutraceutical and food industries as a potential source of natural antioxidants. PMID:25157400

  14. Petit suisse from black soybean: bioactive compounds and antioxidant properties during development process.

    PubMed

    de Moraes Filho, Marsilvio Lima; Hirozawa, Sabrina Satie; Prudencio, Sandra Helena; Ida, Elza Iouko; Garcia, Sandra

    2014-06-01

    This study aimed to evaluate the antioxidant properties, bioactive compounds and other physico-chemical parameters from black soybean and its derivatives over 30?days under refrigeration at 4?°C and develop a probiotic petit suisse produced from black soybean. The soymilk showed the highest levels of isoflavones (109?mg/100?g), total phenolics (600?mg/100?g) and total anthocyanins (388?mg/100?g) with the highest response in the tests with DPPH• and ABTS+• on a dry basis. There was a significant increase (p???0.05) in antioxidant activity during storage due to the hydrolysis of isoflavone glycosides to aglycones in soymilk sample, having a strong linear correlation between the concentration of isoflavone aglycones and the antioxidant activity for ABTS+• (R?=?0.9437, 0.9624 and 0.9992) and DPPH• (R?=?0.9865, 0.9978 and 0.9911), respectively, for soymilk, quark and petit suisse. The conversion of isoflavone was influenced directly by the characteristics of each sample, inhibiting or promoting the action of the enzyme. The petit suisse developed is an alternative for consumers, providing isoflavones and anthocyanins, possessing probiotic average counts (10??CFU?g?¹) during storage. PMID:24467602

  15. Phenolic compounds and antioxidant properties of breeding lines between the white and black rice.

    PubMed

    Zhang, Hongcheng; Shao, Yafang; Bao, Jinsong; Beta, Trust

    2015-04-01

    Advanced breeding lines made from the cross between the black and white rice as parents were collected to evaluate phenolic levels and antioxidant properties. No free phenolic acid was found in the soluble fraction, while p-coumaric acid, ferulic acid, isoferulic acid and vanillic acid were identified in insoluble bound fractions. Of noteworthy, is isoferulic acid which has rarely been reported to occur in cereal grains. Phenolic dehydrodimers were only observed in the insoluble bound fractions, which mainly consisted of 8-5'-coupled diferulic acids and 5-5'-coupled diferulic acids. Cyanidin 3-glucoside, peonidin 3-glucoside and cyanidin occurred in black and some light-purple rice samples. The breeding line YF53 has the highest total phenolic content (23.3mg ferulic acid equiv./g), total anthocyanin content (2.07 mg cyanidin-3-glu equiv./g), and antioxidant activities. The results indicate that it is possible to develop advanced breeding lines for improvement of the phenolic profiles and antioxidant capacity with high yield. PMID:25442600

  16. Comparative study of antioxidant properties of wild growing and cultivated Allium species.

    PubMed

    Stajner, D; Igi?, R; Popovi?, B M; Malenci?, Dj

    2008-01-01

    Allium species are cultivated for the edible bulb, which is used mainly as flavoring in foods. Besides that, they could prevent tumor promotion and some processes that are associated with free radicals, such as cardiovascular diseases and aging. Therefore, different Allium species, both cultivated (Allium nutans L., A. fistulosum L., A. vineale L., A. pskemense B. Fedtsch, A. schoenoprasum L., A. cepa L. and A. sativum L.) and wild (A. flavum L., A. sphaerocephalum L., A. atroviolaceum Boiss, A. vineale L., A. ursinum L., A. scorodoprasum L., A. roseum L. and A. subhirsutum L.), were investigated in order to evaluate the antioxidant properties of their bulbs. This study reports on the results obtained for the bulb antioxidant enzyme activities (superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase), the quantities of non-enzymatic plant antioxidants (reduced glutathione and total flavonoids), the contents of soluble proteins, vitamin C, carotenoids, chlorophylls a and b, as well as for the quantities of malonyldialdehyde and .OH and O2.- radicals. PMID:17726730

  17. Polyphenol oxidase activity and antioxidant properties of Yomra apple (Malus communis L.) from Turkey.

    PubMed

    Can, Zehra; Dincer, Barbaros; Sahin, Huseyin; Baltas, Nimet; Yildiz, Oktay; Kolayli, Sevgi

    2014-12-01

    In this study, firstly, antioxidant and polyphenol oxidase (PPO) properties of Yomra apple were investigated. Seventeen phenolic constituents were measured by reverse phase-high-performance liquid chromatography (RP-HPLC). Total phenolic compounds (TPCs), ferric reducing antioxidant power (FRAP) and 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activities were performed to measure antioxidant capacity. Some kinetic parameters (Km, Vmax), and inhibition behaviors against five different substrates were measured in the crude extract. Catechin and chlorogenic acid were found as the major components in the methanolic extract, while ferulic acid, caffeic acid, p-hydroxybenzoic acid, quercetin and p-coumaric acid were small quantities. Km values ranged from 0.70 to 10.10 mM in the substrates, and also 3-(4-hydroxyphenyl) propanoic acid (HPPA) and L-DOPA showed the highest affinity. The inhibition constant of Ki were ranged from 0.05 to 14.90 mM against sodium metabisulphite, ascorbic acid, sodium azide and benzoic acid, while ascorbic acid and sodium metabisulphite were the best inhibitors. PMID:24246090

  18. The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin, and allyl disulfide.

    PubMed

    Chung, Lip Yong

    2006-01-01

    Garlic and garlic extracts, through their antioxidant activities, have been reported to provide protection against free radical damage in the body. This study investigated antioxidant properties of garlic compounds representing the four main chemical classes, alliin, allyl cysteine, allyl disulfide, and allicin, prepared by chemical synthesis or purification. Alliin scavenged superoxide, while allyl cysteine and allyl disulfide did not react with superoxide. Allicin suppressed the formation of superoxide by the xanthine/xanthine oxidase system, probably via a thiol exchange mechanism. Alliin, allyl cysteine, and allyl disulfide all scavenged hydroxyl radicals; the rate constants calculated based on deoxyribose competitive assay were 1.4-1.7 x 10(10), 2.1-2.2 x 10(9), and 0.7-1.5 x 10(10) M (1) second(1), respectively. Contrary to previous reports, allicin did not exhibit hydroxyl radical scavenging activity in this study. Alliin, allicin, and allyl cysteine did not prevent induced microsomal lipid peroxidation, but both alliin and allyl cysteine were hydroxyl scavengers, and allyl disulfide was a lipid peroxidation terminator. In summary, our findings indicated that allyl disulfide, alliin, allicin, and allyl cysteine exhibit different patterns of antioxidant activities as protective compounds against free radical damage. PMID:16822206

  19. Antioxidant properties of extracts of wild medicinal mushroom species from Croatia.

    PubMed

    Piljac-Zegarac, Jasenka; Samec, Dunja; Piljac, Ante; Mesi?, Armin; Tkalcec, Zdenko

    2011-01-01

    Antioxidant activity and total phenol (TP) content of methanol and water extracts of three wild Croatian mushroom species Auricularia auricula-judae (Bull.) Quél., Sarcoscypha austriaca (Sacc.) Boud., and Strobilurus esculentus (Wulfen) Singer were determined and compared with the values obtained for extracts of four cultivated mushrooms Agaricus bisporus (J.E. Lange) Imbach (brown and white strains), Pleurotus ostreatus (Jacq.) P. Kumm., and Lentinus edodes (Berk.) Singer. Spectrophotometric determination of the TP content was performed using the Folin-Ciocalteu method, while antioxidant activity was measured in a reaction with 1,1-diphenyl-2-picrylhydrazyl radical (DPPH assay) and ferric-tripyridyltriazine (Fe3+-TPTZ) complex (FRAP assay). On the average, 5.8-fold higher TP content was observed for water in comparison to methanol extracts of all analyzed mushrooms. Consequently, antioxidant activity was also higher for water extracts, which is evident from the obtained higher values in the FRAP assay and lower EC50 values in the DPPH assay. Among the three tested wild species, the water extract of S. esculentus exhibited the highest concentration of TP, 8.12 mg/g gallic acid equivalents (GAE), the highest reducing power, 19.42 mmol Fe2+/kg, and the best radical scavenging properties, EC50= 13.5 mg/mL. PMID:22135877

  20. Valorization of pomegranate peel from 12 cultivars: dietary fibre composition, antioxidant capacity and functional properties.

    PubMed

    Hasnaoui, Nejib; Wathelet, Bernard; Jiménez-Araujo, Ana

    2014-10-01

    The dried powdered fruit peels of pomegranate (Punica granatum L.) (PomP) from 12 cultivars were used to extract and characterise their dietary fibre (DF) and to assess their functional and antioxidant properties. The total DF content varied between 33.10 and 62/100 g. The cellulose, Klason lignin, uronic acid and total neutral sugars (NS) composition of DF was: 16.53-22.71, 20.59-41.86, 13.98-23.31 and 16.88-19.66/100g, respectively. Arabinose and xylose were the most present NS with more than 60% of total NS content. The ratio of insoluble to soluble DF was around 1, reflecting the balanced composition of PomP's DF. Besides, PomP powder showed intermediate values for water- and oil-holding capacities: 2.31-3.53 and 2.80-4.05 mL/g, respectively, and strong retardation effect on the dialysis of glucose, reaching ?60%. Also, it has been shown that most of the antioxidants can be extracted, based on the strong soluble antioxidant activity (2018-2649 ?mol Trolox/g) compared to the insoluble one (13-23 ?mol Trolox/g). PMID:24799227

  1. Physico-chemical properties, antioxidant activity and mineral contents of pineapple genotypes grown in china.

    PubMed

    Lu, Xin-Hua; Sun, De-Quan; Wu, Qing-Song; Liu, Sheng-Hui; Sun, Guang-Ming

    2014-01-01

    The fruit physico-chemical properties, antioxidant activity and mineral contents of 26 pineapple [Ananas comosus (L.) Merr.] genotypes grown in China were measured. The results showed great quantitative differences in the composition of these pineapple genotypes. Sucrose was the dominant sugar in all 26 genotypes, while citric acid was the principal organic acid. Potassium, calcium and magnesium were the major mineral constituents. The ascorbic acid (AsA) content ranged from 5.08 to 33.57 mg/100 g fresh weight (FW), while the total phenolic (TP) content varied from 31.48 to 77.55 mg gallic acid equivalents (GAE)/100 g FW. The two parameters in the predominant cultivars Comte de Paris and Smooth Cayenne were relative low. However, MD-2 indicated the highest AsA and TP contents (33.57 mg/100 g and 77.55 mg GAE/100 g FM, respectively), and it also showed the strongest antioxidant capacity 22.85 and 17.30 ?mol TE/g FW using DPPH and TEAC methods, respectively. The antioxidant capacity of pineapple was correlated with the contents of phenolics, flavonoids and AsA. The present study provided important information for the further application of those pineapple genotypes. PMID:24959679

  2. Processed tomato products as a source of dietary lycopene: bioavailability and antioxidant properties.

    PubMed

    Rao, A Venket

    2004-01-01

    Oxidative stress is one of the major contributors to increased risk of chronic diseases. A diet rich in tomatoes and tomato products containing lycopene, a carotenoid antioxidant, has been found to protect against these chronic diseases by mitigating oxidative damage. The study aim was to evaluate the effects of a long-term tomato-rich diet, consisting of various processed tomato products, on bioavailability and antioxidant properties of lycopene. Seventeen healthy human subjects (ten men, seven non-pregnant women) participated in the study. Following a two-week washout period during which subjects avoided foods containing lycopene, all subjects consumed test tomato products including tomato juice, tomato sauce, tomato paste, ketchup, spaghetti sauce, and ready-to-serve tomato soup providing 30 mg of lycopene a day for four weeks. At the end of treatment, serum lycopene level increased significantly (p <0.05), from 181.79 +/- 31.25 to 684.7 +/- 113.91 nmol/L. Similarly, total antioxidant potential increased significantly (p <0.05), from 2.26 +/- 0.015 to 2.38 +/- 0.17 mmol/L Trolox equivalent. Lipid and protein oxidation was reduced significantly (p <0.05). The results suggest that a tomato-rich diet containing different sources of lycopene can increase serum lycopene levels and reduce oxidative stress effectively. PMID:15596034

  3. Antioxidant and ?-Amylase Inhibitory Property of Phyllanthus virgatus L.: An In Vitro and Molecular Interaction Study

    PubMed Central

    Hashim, Arshya; Khan, M. Salman; Khan, Mohd. Sajid; Baig, Mohd. Hassan

    2013-01-01

    The present study on Phyllanthus virgatus, known traditionally for its remedial potential, for the first time provides descriptions of the antioxidant and inhibition of ?-amylase enzyme activity first by in vitro analyses, followed by a confirmatory in silico study to create a stronger biochemical rationale. Our results illustrated that P. virgatus methanol extract exhibited strong antioxidant and oxidative DNA damage protective activity than other extracts, which was well correlated with its total phenolic content. In addition, P. virgatus methanol extract strongly inhibited the ?-amylase activity (IC50 33.20 ± 0.556??g/mL), in a noncompetitive manner, than acarbose (IC50 76.88 ± 0.277??g/mL), which showed competitive inhibition. Moreover, this extract stimulated the glucose uptake activity in 3T3-L1 cells and also showed a good correlation between antioxidant and ?-amylase activities. The molecular docking studies of the major bioactive compounds (9,12-octadecadienoic acid, asarone, 11-octadecenoic acid, and acrylic acid) revealed via GC-MS analysis from this extract mechanistically suggested that the inhibitory property may be due to the synergistic effect of these bioactive compounds. These results provide substantial basis for the future use of P. virgatus methanol extract and its bioactive compound in in vivo system for the treatment and management of diabetes as well as in the related condition of oxidative stress. PMID:23957001

  4. Cultivar variations in antioxidant and antihyperlipidemic properties of pomelo pulp (Citrus grandis [L.] Osbeck) in Thailand.

    PubMed

    Mäkynen, Kittana; Jitsaardkul, Sritanaporn; Tachasamran, Pansiree; Sakai, Nathaporn; Puranachoti, Supitcha; Nirojsinlapachai, Natthapat; Chattapat, Vipaporn; Caengprasath, Natarin; Ngamukote, Sathaporn; Adisakwattana, Sirichai

    2013-08-15

    Pomelo (Citrus grandis L. Osbeck) is a native fruit of great economic importance in Southeast Asia. To provide experimental evidence for the antioxidant and antihyperlipidemic properties of pomelo, 6 cultivars, including Kao-Yai (KY), Thong-dee (TD), Kao-Tangkwa (KT), Kao-Numpueng (KN), Ta-Koi (TK), and Tubtim Siam (TS) were evaluated. KY had the highest phenolic content, and the strongest 1,1-diphenyl-2-pireyhydrazyl radical scavenging capacity and hydroxyl radical scavenging activity. From the high-performance liquid chromatography analysis, naringin and naringenin were the major flavonoids in the KT and TK cultivars. Six pomelo cultivars had antihyperlipidemic activities including the inhibition of pancreatic lipase and cholesterol esterase, as well as cholesterol micelle formation and bile acid binding. Hierarchical clustering analysis showed that the 6 cultivars were separated into 2 classifications. In addition, the total phenolics of the pomelo cultivars were significantly correlated with ferric reducing antioxidant power and Trolox equivalent antioxidant capacity. The results suggest that pomelo provides significant health benefits and may be used for developing functional foods. PMID:23561168

  5. The antioxidant and antigenotoxic properties of citrus phenolics limonene and naringin.

    PubMed

    Bacanl?, Merve; Ba?aran, A Ahmet; Ba?aran, Nur?en

    2015-07-01

    Phenolic compounds not only contribute to the sensory qualities of fruits and vegetables but also exhibit several health protective properties. Limonene and naringin are the most popular phenolics found in Citrus plants. In this study, we investigated the antioxidant capacities of limonene and naringin by the trolox equivalent antioxidant capacity (TEAC) assay and the cytotoxic effects by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in Chinese hamster fibroblast (V79) cells. The genotoxic potentials of limonene and naringin were evaluated by micronucleus (MN) and alkaline COMET assays in human lymphocytes and V79 cells. Limonene and naringin, were found to have antioxidant activities at concentrations of 2-2000?µM and 5-2000?µM respectively. IC50 values of limonene and naringin were found to be 1265?µM and 9026?µM, respectively. Limonene at the concentrations below 10,000?µM and naringin at the all concentrations studied, have not exerted genotoxic effects in lymphocytes and in V79 cells. Limonene and naringin at all concentrations revealed a reduction in the frequency of MN and DNA damage induced by H2O2. PMID:25896273

  6. Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves.

    PubMed

    Yuan, Qingxia; Xie, Yufeng; Wang, Wei; Yan, Yuhua; Ye, Hong; Jabbar, Saqib; Zeng, Xiaoxiong

    2015-09-01

    Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry leaves (MLP) were investigated in the present study. The optimal extraction conditions with an extraction yield of 10.0±0.5% for MLP were determined as follows: extraction temperature 92°C, extraction time 3.5h and ratio (v/w, mL/g) of extraction solvent (water) to raw material 34. Two purified fractions, MLP-3a and MLP-3b with molecular weights of 80.99 and 3.64kDa, respectively, were obtained from crude MLP by chromatography of DEAE-Cellulose 52 and Sephadex G-100. Fourier transform-infrared spectroscopy revealed that crude MLP, MLP-3a and MLP-3b were acidic polysaccharides. Furthermore, crude MLP and MLP-3a had more complicated monosaccharide compositions, while MLP-3b had a relatively higher content of uronic acid. Crude MLP, MLP-3a and MLP-3b exhibited potent Fe(2+) chelating power and scavenging activities on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide and 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid) radicals. The results suggested that MLP could be explored as natural antioxidant. PMID:26005139

  7. Antimicrobial, antioxidant, cytotoxic and molecular docking properties of N-benzyl-2,2,2-trifluoroacetamide

    NASA Astrophysics Data System (ADS)

    Balachandran, C.; Kumar, P. Saravana; Arun, Y.; Duraipandiyan, V.; Sundaram, R. Lakshmi; Vijayakumar, A.; Balakrishna, K.; Ignacimuthu, S.; Al-Dhabi, N. A.; Perumal, P. T.

    2015-02-01

    N-Benzyl-2,2,2-trifluoroacetamide was obtained by acylation of benzylamine with trifluoroacetic anhydride using Friedel-Crafts acylation method. The synthesised compound was confirmed by spectroscopic and crystallographic techniques. N-Benzyl-2,2,2 -trifluoroacetamide was assessed for its antimicrobial, antioxidant, cytotoxic and molecular docking properties. It showed good antifungal activity against tested fungi and moderate antibacterial activity. The minimum inhibitory concentration values of N-benzyl-2,2,2 -trifluoroacetamide against fungi were 15.62 ?g/mL against A. flavus, 31.25 ?g/mL against B. Cinerea and 62.5 ?g/mL against T. mentagrophytes, Scopulariopsis sp., C. albicans and M. pachydermatis. N-Benzyl-2,2,2-trifluoroacetamide showed 78.97 ± 2.24 of antioxidant activity at 1,000 ?g/mL. Cupric ion reducing antioxidant capacity of N-benzyl-2,2,2-trifluoroacetamide was dependent on the concentration. Ferric reducing antioxidant power assay of N-benzyl-2,2,2-trifluoroacetamide showed (1.352 ± 0.04 mM Fe(II)/g) twofold higher value compared to the standard. N-Benzyl-2,2,2-trifluoroacetamide showed 75.3 % cytotoxic activity at the dose of 200 ?g/mL with IC50 (54.7 %) value of 100 ?g/mL. N-Benzyl-2,2,2-trifluoroacetamide was subjected to molecular docking studies for the inhibition AmpC beta-lactamase, Glucosamine-6-Phosphate Synthase and lanosterol 14 alpha-demethylase (CYP51) enzymes which are targets for antibacterial and antifungal drugs. Docking studies of N-benzyl-2,2,2-trifluoroacetamide showed low docking energy. N-Benzyl-2,2,2-trifluoroacetamide can be evaluated further for drug development.

  8. The antioxidant and anti-cadmium toxicity properties of garlic extracts

    PubMed Central

    Boonpeng, Suwannaporn; Siripongvutikorn, Sunisa; Sae-wong, Chutha; Sutthirak, Pornpong

    2014-01-01

    Cadmium (Cd) contamination is a highly dangerous international problem because it can transfer into the food chain and become bioaccumulated, endangering human health. Cd detoxication is very interesting particularly the method providing no undesirable side effects. Cd also causes lipid oxidation that leads to undesired food quality. Garlic (Allium sativum L.) has been used as conventional food and in herbal therapy and folklore medicine as an antibacterial, antitumorogenic, and antioxidant agent for over 5000 years. In the present work, fresh garlic and pickled garlic extracted with distilled water was brought to determine antioxidant activities in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, 2,2?-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging assay, ferric reducing ability power (FRAP) assay, chelating activities, superoxide, and hydroxyl scavenging assay. The data showed that pickled garlic extracts significantly possessed more DPPH, ABTS, FRAP, superoxide, and hydroxyl scavenging assays as 11.86, 13.74, 4.9, 46.67, and 15.33 g trolox equivalent/g sample, respectively, compared with fresh one as 7.44, 7.62, 0.01, 4.07, and 8.09 g trolox equivalent/g sample, respectively. However, iron chelating activity of fresh garlic extract was higher than that of pickled garlic while there was no significant difference in the copper chelating activity of both extracts. For anti-Cd properties, pickled garlic was more effective than fresh garlic and contained less toxicity than standard diallyl disulfide (DADS). Therefore, therapeutic properties of pickled garlic favored its consumption compared with fresh and standard DADS for its antioxidant and anti-Cd properties. PMID:25493198

  9. The antioxidant and anti-cadmium toxicity properties of garlic extracts.

    PubMed

    Boonpeng, Suwannaporn; Siripongvutikorn, Sunisa; Sae-Wong, Chutha; Sutthirak, Pornpong

    2014-11-01

    Cadmium (Cd) contamination is a highly dangerous international problem because it can transfer into the food chain and become bioaccumulated, endangering human health. Cd detoxication is very interesting particularly the method providing no undesirable side effects. Cd also causes lipid oxidation that leads to undesired food quality. Garlic (Allium sativum L.) has been used as conventional food and in herbal therapy and folklore medicine as an antibacterial, antitumorogenic, and antioxidant agent for over 5000 years. In the present work, fresh garlic and pickled garlic extracted with distilled water was brought to determine antioxidant activities in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging assay, ferric reducing ability power (FRAP) assay, chelating activities, superoxide, and hydroxyl scavenging assay. The data showed that pickled garlic extracts significantly possessed more DPPH, ABTS, FRAP, superoxide, and hydroxyl scavenging assays as 11.86, 13.74, 4.9, 46.67, and 15.33 g trolox equivalent/g sample, respectively, compared with fresh one as 7.44, 7.62, 0.01, 4.07, and 8.09 g trolox equivalent/g sample, respectively. However, iron chelating activity of fresh garlic extract was higher than that of pickled garlic while there was no significant difference in the copper chelating activity of both extracts. For anti-Cd properties, pickled garlic was more effective than fresh garlic and contained less toxicity than standard diallyl disulfide (DADS). Therefore, therapeutic properties of pickled garlic favored its consumption compared with fresh and standard DADS for its antioxidant and anti-Cd properties. PMID:25493198

  10. Effect of green tea supplementation on the microbiological, antioxidant, and sensory properties of probiotic milks.

    PubMed

    Najgebauer-Lejko, Dorota

    2014-01-01

    Green tea and its constituents are known for a wide range of health-promoting properties. They may exert antimicrobial action but without altering lactic acid bacteria. The aim of the present study was to estimate the effect of green tea addition on the selected properties of probiotic milks. Bioyogurts (fermented with ABT-1 coculture of Streptococcus thermophilus, Lactobacillus acidophilus LA-5, Bifidobacterium animalis subsp. lactis BB-12) and acidophilus milks (fermented with pure L. acidophilus LA-5 culture) with addition of 0, 5, 10, or 15% (v/v) green tea infusion (GTI) were produced and analyzed for the antioxidant capacity by the "diphenyl picrylhydrazyl" (DPPH) and "ferric-reducing antioxidant power" (FRAP) methods, acidity, the count of starter bacteria, and sensory properties at the 1st, 7th, 14th, and 21st day of cold storage. The 15% addition of GTI to the acidophilus milk significantly reduced the lactic acid production during the whole study. The GTI had no impact on the level of S. thermophilus and B. lactis BB-12 in bioyogurts, and its effect on the count of L. acidophilus LA-5 depended on the concentration and probiotic milk type. GTI similarly and in a dose-dependent manner enhanced the antioxidant capacity of both milk types. There were no significant differences between the sensory notes received for bioyogurts, whereas acidophilus milks with tea were less appreciated by the panelists. In conclusion, green tea could be successfully used as a functional additive for selected probiotic milks enhancing their health benefits, but the proper selection of tea additive and starter culture is recommended. PMID:24883178

  11. Response of Bacillus sp. F26 to Different Reactive Oxygen Species Stress Characterized by Antioxidative Enzymes Synthesis

    Microsoft Academic Search

    Guoliang Yan; Zhaozhe Hua; Guocheng Du; Jian Chen

    2008-01-01

    The oxidative response of Bacillus sp F26 to different forms of reactive oxygen species (ROS) stress including H2O2, O2? ? ? ? ? ?and OH· was investigated using diverse generating source of ROS, which was characterized by the synthesis of antioxidative enzymes. It was seen that the responses of cells to oxidative stress are largely dependent on species, mode (shock

  12. Synthese and characterization of boronic acid functionalized macroporous uniform poly(4-chloromethylstyrene-co-divinylbenzene) particles and its use in the isolation of antioxidant compounds from plant extracts.

    PubMed

    Cetinkaya, Onur; Duru, Mehmet Emin; Ciçek, Hüseyin

    2012-11-15

    Aminophenyl boronic acid (APBA) carrying uniform-macroporous poly(chloromethylstyrene-co-divinylbenzene), poly(CMS-co-DVB) particles were synthesized for selective separation of cis-diol-containing flavonoids from plant extracts. For this purpose, 2.5 ?m polystyrene seed particles were first swelled by a mixture of dibutyl phthalate (DBP), toluene and dodecanol, then by a monomer mixture including CMS and DVB. The repolymerization of the monomer phase in the swollen seed particles provided macroporous and uniform particles, approximately 7 ?m in size. Chlorine atoms on the surface of these particles were derivatized with APBA to gain affinity properties for flavonoids containing vicinal hydroxyl groups. Model adsorption studies showed that these particles selectively adsorbed quercetin and rutin containing cis-diol groups, but did not adsorb apigenin similar to quercetin and not carrying cis-diol groups. These particles were also tested in adsorption/desorption studies for ethanol and ethyl acetate extracts of the Hypericum perforatum (HP) stems to obtain high antioxidant mixtures. With ethanol extract, the antioxidant activity of the desorption solution was a bit higher than that of the post-adsorption solutions. However, the DPPH radical scavenging activity of the desorption solution decreased with respect to the original extract and post-adsorption solutions. A similar result was obtained for the antioxidant activity of the desorption solution using ethyl acetate extract. An interesting result was obtained that DPPH radical scavenging activity of the post-adsorption solution was higher than that of the original ethyl acetate extract and desorption solutions. These results were attributed to selective adsorption of antioxidant characterized cis-diol-containing apolar molecules much more rather than that radical scavenger characterized polar molecules. PMID:23153644

  13. Anticholinesterase and Antioxidative Properties of Aqueous Extract of Cola acuminata Seed In Vitro

    PubMed Central

    Akinyemi, Ayodele J.; Omojokun, Olasunkanmi S.; Oyeleye, Idowu S.

    2014-01-01

    Background. Cola acuminata seed, a commonly used stimulant in Nigeria, has been reportedly used for the management of neurodegenerative diseases in folklore without scientific basis. This study sought to investigate the anticholinesterase and antioxidant properties of aqueous extracts from C. acuminata seed in vitro. Methodology. The aqueous extract of C. acuminata seed was prepared (w/v) and its effect on acetylcholinesterase (AChE) and butyrylcholinesterase activities, as well as some prooxidant (FeSO4, sodium nitroprusside (SNP), and quinolinic acid (QA)) induced lipid peroxidation in rat brain in vitro, was investigated. Results. The results revealed that C. acuminata seed extract inhibited AChE (IC50 = 14.6 ?g/mL) and BChE (IC50 = 96.2 ?g/mL) activities in a dose-dependent manner. Furthermore, incubation of rat's brain homogenates with some prooxidants caused a significant increase P < 0.05 in the brain malondialdehyde (MDA) content and inhibited MDA production dose-dependently and also exhibited further antioxidant properties as typified by their high radicals scavenging and Fe2+ chelating abilities. Conclusion. Inhibition of AChE and BChE activities has been the primary treatment method for mild Alzheimer's disease (AD). Therefore, one possible mechanism through which the seed exerts its neuroprotective properties is by inhibiting cholinesterase activities as well as preventing oxidative-stress-induced neurodegeneration. However, this is a preliminary study with possible physiological implications. PMID:25506036

  14. Gamma irradiation induced modification of bean polysaccharides: impact on physicochemical, morphological and antioxidant properties.

    PubMed

    Hussain, Peerzada R; Wani, Idrees A; Suradkar, Prashant P; Dar, Mohd A

    2014-09-22

    In the present study starches from four bean varieties viz. red, yellow, black and white, were gamma irradiated in the dose range of 5-25 kGy to investigate the effect of radiation processing on physicochemical, morphological and antioxidant properties. Studies revealed positive correlation between gamma irradiation and solubility (r=0.91), irradiation and water absorption capacity (r=0.82) and negative correlations between irradiation and swelling power (r=-0.92), irradiation and pasting properties (r=-0.91) and irradiation and thermal properties (r=-0.89). Microscopic observation under scanning electron microscope indicated the development of surface cracking and fractures on the surface of starch granules with increase in dose. X-ray diffractometry revealed no significant change in diffraction patterns between control and irradiated starches, except a decrease in relative crystallinity. Irradiation increased the proportions of both rapidly digestible starch and enzyme resistant starch of bean starches and significantly prevented the retrogradation of bean starches during storage. Results of the DPPH radical scavenging activity and ferric reducing power indicated significant (p?0.05) increase in antioxidant activity of all irradiated bean starches with increase in dose. PMID:24906745

  15. Single-step green synthesis and characterization of gold-conjugated polyphenol nanoparticles with antioxidant and biological activities

    PubMed Central

    Sanna, Vanna; Pala, Nicolino; Dessì, Giuseppina; Manconi, Paola; Mariani, Alberto; Dedola, Sonia; Rassu, Mauro; Crosio, Claudia; Iaccarino, Ciro; Sechi, Mario

    2014-01-01

    Background Gold nanoparticles (GNPs) are likely to provide an attractive platform for combining a variety of biophysicochemical properties into a unified nanodevice with great therapeutic potential. In this study we investigated the capabilities of three different natural polyphenols, epigallocatechin-3-gallate (EGCG), resveratrol (RSV), and fisetin (FS), to allow synergistic chemical reduction of gold salts to GNPs and stabilization in a single-step green process. Moreover, antioxidant properties of the nanosystems, as well as preliminary antiproliferative activity and apoptotic process investigation of model EGCG-GNPs on stable clones of neuroblastoma SH-SY5Y cells expressing CFP-DEVD-YFP reporter, were examined. Methods The GNPs were characterized by physicochemical techniques, polyphenol content, and in vitro stability. The antioxidant activity of the GNPs was also determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2?-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation (ABTS) radical-scavenging assays. Stable clones of neuronal SH-SY5Y-CFP-DEVD-YFP were generated and characterized, and cell viability after treatment with EGCG-GNPs was assessed after 72 hours through a 3(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. Activation of the apoptotic pathways was also investigated by Western blot analysis. Results With a diameter in the size range of 10–25 nm, the obtained nanoparticles (NPs) were found to contain 2.71%, 3.23%, and 5.47% of EGCG, RSV, and FS, respectively. Nanoprototypes exhibited remarkable in vitro stability in various media, suggesting that NP surface coating with phytochemicals prevents aggregation in different simulated physiological conditions. The scavenging activities for DPPH and ABTS were highly correlated with EGCG, RSV, and FS content. Moreover, high correlation coefficients between the ABTS and DPPH values were found for the prepared nanosystems. EGCG-GNPs induce a dose-dependent reduction on SH-SY5Y-CFP-DEVD-YFP cell viability that is likely to involve the activation of the apoptotic pathways, similarly to free EGCG, as suggested by the processing of the CFP-DEVD-YFP reporter. Conclusion These results prompted us to propose the ecofriendly synthesized EGCG-, RSV-, and FS-based nanogold conjugates as suitable carriers for bioactive polyphenols to be used for the treatment of disorders associated with oxidative stress, including neurodegenerative disorders, cardiovascular disease, and cancer. PMID:25364251

  16. Mechanical Properties Characterization at the Nanoscale

    Microsoft Academic Search

    Hanson Fong; Jeffery Sopp; Mehmet Sarikaya

    2001-01-01

    Nanoindentation is an unique technique that characterizes mechanical properties of materials down to the nanometer scale. With a force range from nanoNewtons to milliNewtons, unique properties of surface structures and thin films in the mesoscale can be routinely quantifieds. With technology continually pushing toward smaller feature size in electronic and mechanical devices as well as biomaterials applications, nanoindentation has become

  17. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis

    Microsoft Academic Search

    Jae-Young Je; Zhong-Ji Qian; Hee-Guk Byun; Se-Kwon Kim

    2007-01-01

    To utilize fish processing waste, tuna backbone protein was hydrolyzed using different proteases (alcalase, ?-chymotrypsin, neutrase, papain, pepsin and trypsin) for production of antioxidant peptide. Antioxidant activities of hydrolysates were evaluated using lipid peroxidation inhibition assay and direct free radical scavenging activity by using electron spin resonance (ESR) spectrometer. Among hydrolysates, peptic hydrolysate exhibited the highest antioxidant activity compared to

  18. Functional and antioxidant properties of novel snack crackers incorporated with Hibiscus sabdariffa by-product.

    PubMed

    Ahmed, Zahra S; Abozed, Safaa S

    2015-01-01

    The Hibiscus sabdariffa calyxes' residue (HSR) remained after the extraction of beverage is discarded which contributes to environmental pollution. The objective of this study was to explore the suitability of incorporating different amount of HSR (0%, 1.25%, 2.5%, 3.75%, and 5.0%) in crackers to enhance dietary fiber and antioxidant content. Physicochemical properties, antioxidants activity, nutritional quality, sensory profile and microstructure properties of samples containing HSR were examined and compared with control crackers. Cracker protein and fat levels decreased as HSR increased from 0.0% to 5% while ash increased. The total dietary fiber DF increased from 3.36% to 8.17% where the highest DF was reached at 5% HSR. The content of phenols increased from 5.99 to 17.57 mg/g and total flavonoid content increased from 49.36 to 104.63 mg/g of crackers incorporated with 5% HSR. DPPH radical scavenging activity increased two fold by increasing HSR up to 5%. HSR containing crackers exhibited darker L values than none/less HSR containing ones. In sensory ranking tests, acceptable crackers with pleasant flavor were obtained by incorporating up to 3.75% HSR into the cracker's formula. Crackers prepared with 5% HSR received the poorest sensory rating compared to non/less HSR enriched cracker. Scanning electron microscopy (EM) images of the prepared crackers revealed marked changes caused by incorporating HSR as upon HSR addition the surface was observed to be scratched, cracker and rougher. Overall results suggest that HSR is a potential functional food ingredient high in fiber content and antioxidants activity that may be processed into flour and used in food applications, such as baked goods. PMID:25685546

  19. THIOCYANATE: A potentially useful therapeutic agent with host defense and antioxidant properties?

    PubMed Central

    Chandler, Joshua D.; Day, Brian J.

    2014-01-01

    Thiocyanate (SCN) functions in host defense as part of the secreted lactoperoxidase (LPO) microbicidal pathway. SCN is the preferred substrate for LPO-driven catalytic reduction of hydrogen peroxide (H2O2) forming hypothiocyanous acid (HOSCN). HOSCN is selectively generated by many peroxidase enzymes that can utilize SCN including: eosinophil peroxidase (EPO), gastric peroxidase (GPO), myeloperoxidase (MPO), salivary peroxidase (SPO), and thyroid peroxidase (TPO). These enzymes generate HOSCN through a two-electron halogenation reaction. HOSCN is a potent microbicidal agent that kills or nullifies invading pathogens but is better tolerated by host tissue. Some controversy exists as to whether physiologic levels of HOSCN are non-toxic to host tissue, but the disagreement appears to be based on results of enzymatic generation (yielding moderate steady-state exposure) versus direct high level acute exposure in mammalian cell lines. This apparent duality is also true of other endogenous oxidants such as hydrogen peroxide and relates to the difference between physiologically relevant oxidant production versus supra-physiologic bolus dosing approaches. SCN has antioxidant properties that include the ability to protect cells against oxidizing agents such as hypochlorous acid (HOCl) and repair protein chloramines. SCN is an important endogenous molecule that has the potential to interact in complex and elegant ways with its host environment and foreign organisms. SCN’s diverse properties as both host defense and antioxidant agent make it a potentially useful therapeutic. PMID:22968041

  20. Accelerated Characterization of Polymer Properties

    SciTech Connect

    R. Wroczynski; l. Brewer; D. Buckley; M. Burrell; R. Potyrailo

    2003-07-30

    This report describes the efforts to develop a suite of microanalysis techniques that can rapidly measure a variety of polymer properties of industrial importance, including thermal, photo-oxidative, and color stability; as well as ductility, viscosity, and mechanical and antistatic properties. Additional goals of the project were to direct the development of these techniques toward simultaneous measurements of multiple polymer samples of small size in real time using non-destructive and/or parallel or rapid sequential measurements, to develop microcompounding techniques for preparing polymers with additives, and to demonstrate that samples prepared in the microcompounder could be analyzed directly or used in rapid off-line measurements. These enabling technologies are the crucial precursors to the development of high-throughput screening (HTS) methodologies for the polymer additives industry whereby the rate of development of new additives and polymer formulations can be greatly accelerated.

  1. Isolation and characterization of phenolic compounds and anthocyanins from Murta (Ugni molinae Turcz.) fruits. Assessment of antioxidant and antibacterial activity.

    PubMed

    Junqueira-Gonçalves, Maria Paula; Yáñez, Lina; Morales, Carolina; Navarro, Muriel; A Contreras, Rodrigo; Zúñiga, Gustavo E

    2015-01-01

    Berry fruit consumption has become important in the promotion of human health, mainly due to their phenolic compounds, which have been associated with protection against different pathologies, as well as antimicrobial and other biological activities. Consequently, there has been a growing interest in identifying natural antioxidants and antimicrobials from these plants. This study aimed to characterize the phenolic chemical composition and anthocyanin profile of murta (Ugni molinae Turcz.) fruit, and to evaluate the antioxidant and antimicrobial activity of its extracts (ethanolic and methanolic). LC/MS of the ethanolic extracts showed the presence of three major compounds: caffeic acid 3-glu, quercetin-3-glu and quercetin, while in the methanolic acid extract they were cyanidin-3-glucoside, pelargonidin-3-arabinose and delphinidin-3-glucoside. The antioxidant activity of ethanolic extracts (DPPH· and ORAC assays) was higher than that of methanol acid extracts or purified anthocynins. Furthermore, the methanol acid extract showed an inhibitory activity against the bacteria E. coli and S. typhi similar to that of standard antibiotics. The results suggest that the antioxidant activity of the ethanolic extract is regulated by the high content of phenolic compounds and the fruit's characteristic color is due to the content of pelargonidin-3-arabinose and delphinidin-3-glucoside. The obtained results demonstrated the appreciable antioxidant and antibacterial activities, providing opportunities to explore murta extracts as biopreservatives. PMID:25838172

  2. Propolis as potential cosmeceutical sunscreen agent for its combined photoprotective and antioxidant properties.

    PubMed

    Gregoris, Elena; Fabris, Sabrina; Bertelle, Mariangela; Grassato, Luigi; Stevanato, Roberto

    2011-02-28

    Propolis, bee glue, and its main polyphenolic components show high antioxidant activity as found measuring their inhibitory action on lipid peroxidation of linoleic acid (LA) in sodium dodecyl sulfate (SDS) micelles. Furthermore, these substances evidence effectiveness as broad spectrum UVB and UVA photoprotection sunscreens, as it results by measurements of sun protection factor (SPF), the universal indicator related primarily to UVB radiations, and of the two parameters giving an indication of the UVA absorbance properties, i.e. UVA/UVB ratio and critical wavelength. The combination of these characteristics moves up propolis and its main polyphenolic components to the class of cosmeceuticals, as possible active ingredient of sunscreen commercial formulations for their protective and preventive properties. PMID:21134430

  3. Effects of oral dosage form and storage period on the antioxidant properties of four species used in traditional herbal medicine.

    PubMed

    Guimarães, Rafaela; Barreira, João C M; Barros, Lillian; Carvalho, Ana Maria; Ferreira, Isabel C F R

    2011-04-01

    Herbal infusions and decoctions in water are some of the most commonly consumed beverages in the world. Although water is not a good solvent for many of the active components in herbs, liquid preparations are rich in several bioactive compounds. Most of them have powerful antioxidant activity and have been related to medicinal herbs' properties. Herein, decoctions and infusions in water of lemon-verbena (Aloysia citrodora) aerial parts and leaves, fennel (Foeniculum vulgare), pennyroyal (Mentha pulegium) and spearmint (Mentha spicata) aerial parts with different periods of storage (0, 30, 60 and 120 days), were prepared. The effects of the method of preparation and storage period on their antioxidant properties were analysed. For all the analysed species, infusions gave better results than the corresponding decoctions. Spearmint infusions showed the highest antioxidant properties, at all the storage periods, probably due to the highest levels and synergy between phenolics, flavonoids and ascorbic acid found in this sample. Linear discriminant analysis confirmed that the length of storage period has a significant influence on the antioxidant activity and antioxidant content. Flavonoids and reducing sugars proved to be the parameters that most highly contributed to cluster individual groups according to different periods of storage. PMID:20740475

  4. Synthesis, characterization and antioxidant activity of a novel electroactive and biodegradable polyurethane for cardiac tissue engineering application.

    PubMed

    Baheiraei, Nafiseh; Yeganeh, Hamid; Ai, Jafar; Gharibi, Reza; Azami, Mahmoud; Faghihi, Faezeh

    2014-11-01

    There has been a growing trend towards applying conducting polymers for electrically excitable cells to increase electrical signal propagation within the cell-loaded substrates. A novel biodegradable electroactive polyurethane containing aniline pentamer (AP-PU) was synthesized and fully characterized by spectroscopic methods. To tune the physico-chemical properties and biocompatibility, the AP-PU was blended with polycaprolactone (PCL). The presence of electroactive moieties and the electroactivity behavior of the prepared films were confirmed by UV-visible spectroscopy and cyclic voltammetry. A conventional four probe analysis demonstrated the electrical conductivity of the films in the semiconductor range (~10(-5)S/cm). MTT assays using L929 mouse fibroblast and human umbilical vein endothelial cells (HUVECs) showed that the prepared blend (PB) displayed more cytocompatibility compared with AP-PU due to the introduction of a biocompatible PCL moiety. The in vitro cell culture also confirmed that PB was as supportive as tissue culture plate. The antioxidant activity of the AP-PU was proved using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay by employing UV-vis spectroscopy. In vitro degradation tests conducted in phosphate-buffered saline, pH7.4 and pH5.5, proved that the films were also biodegradable. The results of this study have highlighted the potential application of this bioelectroactive polyurethane as a platform substrate to study the effect of electrical signals on cell activities and to direct desirable cell function for tissue engineering applications. PMID:25280676

  5. Redox properties of the nitronyl nitroxide antioxidants studied via their reactions with nitroxyl and ferrocyanide.

    PubMed

    Bobko, A A; Khramtsov, V V

    2015-08-01

    Nitronyl nitroxides (NNs) are the paramagnetic probes that are capable of scavenging physiologically relevant reactive oxygen (ROS) and nitrogen (RNS) species, namely superoxide, nitric oxide (NO), and nitroxyl (HNO). NNs are increasingly considered as potent antioxidants and potential therapeutic agents. Understanding redox chemistry of the NNs is important for their use as antioxidants and as paramagnetic probes for discriminative detection of NO and HNO by electron paramagnetic resonance (EPR) spectroscopy. Here we investigated the redox properties of the two most commonly used NNs, including determination of the equilibrium and rate constants of their reduction by HNO and ferrocyanide, and reduction potential of the couple NN/hydroxylamine of nitronyl nitroxide (hNN). The rate constants of the reaction of the NNs with HNO were found to be equal to (1-2)× 10(4) M(- 1)s(- 1) being close to the rate constants of scavenging superoxide and NO by NNs. The reduction potential of the NNs and iminonitroxides (INs, product of NNs reaction with NO) were calculated based on their reaction constants with ferrocyanide. The obtained values of the reduction potential for NN/hNN (E'0?285 mV) and IN/hIN (E'?495 mV) are close to the corresponding values for vitamin C and vitamin E, correspondingly. The "balanced" scavenging rates of the NNs towards superoxide, NO, and HNO, and their low reduction potential being thermodynamically close to the bottom of the pecking order of oxidizing radicals, might be important factors contributing into their antioxidant activity. PMID:25789760

  6. Anti-lipase and antioxidant properties of 30 medicinal plants used in Oaxaca, México.

    PubMed

    Villa-Ruano, Nemesio; Zurita-Vásquez, Guilibaldo G; Pacheco-Hernández, Yesenia; Betancourt-Jiménez, Martha G; Cruz-Durán, Ramiro; Duque-Bautista, Horacio

    2013-01-01

    We report the results of in vitro anti-lipase and antioxidant assays using crude ethanolic extracts from 30 plants grown in Oaxaca, México. Anti-lipase tests were performed by using porcine pancreatic lipase (PPL) [EC 3.1.1.3] from Affymetrix/USB. The extracts of Solanum erianthum, Salvia microphylla, Brungmansia suaveolens and Cuphea aequipetala showed up to 60% PPL inhibition. The effect of these extracts on the kinetic parameters of PPL (Km= 0.36 mM, and Vmax=0.085 mM min -1) revealed that the alcoholic preparations of S. erianthum and C. aequipetala engendered a non-competitive inhibition (Vmax=0.055 mM min -1; Vmax= 0.053 mM min -1), whereas those of S. microphylla and B. suaveolens produced a mixed inhibition (Km= 0.567 mM, Vmax=0.051 mM min _1; Km=0.643 mM, Vmax= 0.042 mM min ¹). In addition to these findings, seven extracts from different plants were able to inhibit PPL in the range of 30-50%. Antioxidant tests against 2,2-Diphenyl-1-picryl hydrazyl (DPPH) confirmed that Arctostaphylos pungens, Gnaphalium roseum, Crotalaria pumila, Cuphea aequipetala, Rhus chondroloma, and Satureja laevigata possess relevant antioxidant activity (IC(5)0=50-80 ?g mL¹). The general composition of the most effective ethanolic extracts was obtained in order to confirm their known chemistry reported by previous works. Comprehensive chemical analysis of the ethanolic extracts and their poisoning effects suggests that S. microphylla, C. aequipetala and A. pungens could be considered as the best sources with both desired properties. PMID:23959013

  7. Antioxidant properties of mushroom mycelia obtained by batch cultivation and tocopherol content affected by extraction procedures.

    PubMed

    Vamanu, Emanuel

    2014-01-01

    The determination of the antioxidant potential of lyophilized mushroom mycelia from 5 strains of the species Pleurotus ostreatus and Coprinus comatus (obtained by submerged cultivation in batch system) was analyzed as ethanolic extracts by evaluating ABTS and the hydroxyl scavenging activity, FRAP method, the chelating capacity, the inhibition of human erythrocyte hemolysis, and the inhibition of xanthine oxidase activity. The main compounds present in all extracts were determined by HPLC chromatography. Overall, results demonstrated that the biologically active substances content is modulated by the extraction method used. The most beneficial extract, characterized by determining the EC50 value, was that of C. comatus M8102, followed by P. ostreatus PQMZ91109. Significant amount of ?-tocopherol (179.51?±?1.51?mg/100?g extract) was determined as well as flavones such as rutin and apigenin. In the P. ostreatus PQMZ91109 extract, 4.8?±?0.05?mg/100?g extract of tocopherol acetate known to play a significant role as an antioxidant in skin protection against oxidative stress generated by UV rays was determined. The various correlations (r (2) = 0.7665-0.9426 for tocopherol content) assessed and the composition of extracts in fluidized bed from the mycelia of the tested species depicted a significant pharmacological potential as well as the possibility of usage in the development of new functional products. PMID:25110715

  8. An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles.

    PubMed

    Can, Zehra; Yildiz, Oktay; Sahin, Huseyin; Turumtay, Emine Akyuz; Silici, Sibel; Kolayli, Sevgi

    2015-08-01

    This study investigated some physico-chemical and biochemical characteristics of different honey types belonging to Turkish flora. Sixty-two honey samples were examined on the basis of pollen analyses, including 11 unifloral honeys (chestnut, heather, chaste tree, rhododendron, common eryngo, lavender, Jerusalem tea, astragalus, clover and acacia), two different honeydew honeys (lime and oak), and 7 different multifloral honeys. Electrical conductivity, moisture, Hunter color values, HMF, proline, diastase number, and sugar analyses of the honey samples were assessed for chemical characterization. Some phenolic components were analyzed by reverse phase high performance liquid chromatography (RP-HPLC) to determine honeys' phenolic profiles. Total phenolic compounds, total flavonoids, ferric reducing antioxidant capacity (FRAP) and 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity were measured as antioxidant determinants. The study results confirm that physico-chemical and biological characteristics of honeys are closely related to their floral sources, and that dark-colored honeys such as oak, chestnut and heather, have a high therapeutic potential. PMID:25766810

  9. Properties, synthesis, and characterization of graphene

    Microsoft Academic Search

    Liang-Xu Dong; Qiang Chen

    2010-01-01

    Graphene is a wonder material that attracts great interests in materials science and condensed matter physics. It is the thinnest\\u000a material and also the strongest material ever measured. Its distinctive band structure and physical properties determine its\\u000a bright application prospects. This review introduces briefly the properties and applications of graphene. Recent synthesis\\u000a and characterization methods are summarized in detail, and

  10. Assessing the effects of different pectins addition on color quality and antioxidant properties of blackberry jam

    PubMed Central

    2013-01-01

    Background In the last years pectin and other hydrocolloids were tested for improving the color stability and the retention of bioactive compounds in gelled fruit-based products. In line with these concerns, our study has been directed to quantify the changes in antioxidant status and color indices of blackberry jam obtained with different types of pectin (degree of esterification: DE, degree of amidation: DA) and doses in response to processing and storage for 1, 3 and 6 months at 20°C. Results Blackberry jam was obtained by a traditional procedure used in households or small-scale systems with different commercial pectins (HMP: high-methoxyl pectin, LMP: low-methoxyl pectin and LMAP: low-methoxyl amidated pectin) added to three concentrations (0.3, 0.7 and 1.0%) and investigated in terms of total monomeric anthocyanins (TMA), antioxidant capacity expressed as ferric reducing antioxidant power (FRAP), total phenolics (TP), color density (CD) and percent of polymeric color, PC (%). Thermal processing resulted in significant depreciation of analyzed parameters reported to the corresponding values of fresh fruit as follows: TMA (69-82%), TP (33-55%) and FRAP (18-52%). Biologically active compounds and color were best retained one day post-processing in jams with LMAP followed by samples with LMP and HMP. Storage for 6 months brings along additional dramatic losses reported to the values recorded one day post-processing as follows: TMA (31-56%), TP (29-51%) and FRAP (20-41%). Also, both processing and storage resulted in significant increases in PC (%). The pectin type and dosage are very influential factors for limiting the alterations occurring in response to processing and storage. The best color retention and the highest TMA, TP and FRAP were achieved by LMAP, followed by LMP and HMP. Additionally, a high level of bioactive compounds in jam could be related to a high dose of pectin. LMAP to a level of 1% is the most indicated to provide the highest antioxidant properties in jam. Conclusions The retention of bioactive compounds and jam color stability were strongly dependent on the pectin type and dosage. By a proper selection of pectin type and dose could be limited the losses recorded in response to processing and storage. PMID:23856382

  11. Antioxidant, Anti-inflammatory, and Chemoprotective Properties of Acacia catechu Heartwood Extracts.

    PubMed

    Stohs, Sidney J; Bagchi, Debasis

    2015-06-01

    Aqueous extracts of Acacia catechu heartwood are rich source of catechin and epicatechin (gallic acid derivatives), with smaller amounts of flavonoids. Extracts have also been prepared with ethyl acetate, ethanol, and methanol, and the properties of these extracts have been studied and are reviewed. Potent antioxidant activity has been well established in both in vitro and in vivo studies. This antioxidant activity is believed to be responsible for the anti-inflammatory, tissue protectant, antineoplastic, and analgesic activities that have been demonstrated and clearly established in animal and cell culture systems. Furthermore, antihyperglycemic, antidiarrheal, antinociceptive, and antipyretic activities have been demonstrated in animal studies. No adverse effects have been observed in animal or human studies or in cell culture systems. In spite of the fact that Acacia products have been used for many years and the general safety of catechins and epicatechins is well documented, few human studies have ever been conducted on the efficacy or safety of A.?catechu heartwood extracts. Several studies have shown that a two-ingredient combination product containing A.?catechu extract exhibited no adverse effects when administered daily for up to 12?weeks while exhibiting significant anti-inflammatory activity in subjects with osteoarthritis of the knee. There is a need for additional human clinical studies with regard to efficacy and safety. © 2015 The Authors. Phytotherapy Research published by John Wiley & Sons Ltd. PMID:25802170

  12. Physicochemical properties and storage stability of margarine containing Opuntia ficus-indica peel extract as antioxidant.

    PubMed

    Chougui, Nadia; Djerroud, Naima; Naraoui, Fatima; Hadjal, Samir; Aliane, Khellaf; Zeroual, Brahim; Larbat, Romain

    2015-04-15

    This study falls within the framework of the industrial exploitation of by-products of the prickly pear (Opuntia ficus-indica). The study aims to evaluate the use of hydro-ethanolic extract of prickly pear peels as a substitute of vitamin E used as antioxidant in margarine preservation. The extract was rich in total phenolics (1512.58 mg GAE/100 g DM). HPLC-DAD-ESI-MS(n) analyses allowed the identification of sixteen compounds belonging to hydroxybenzoic acids, hydroxycinnamic acids and flavonoids. The extract displayed a reducing power and an antiradical activity that were respectively similar to and lower than the two antioxidant standards quercetin and butylated hydroxyanisole. Tests conducted at laboratory and pilot scales showed that the margarines elaborated with peel extract were more resistant to oxidation than the margarine reference with vitamin E. In addition, neither the physicochemical nor the microbiological properties were modified. Prickly pear peels contain bioactive substances that could be used in different food sectors. PMID:25466036

  13. Photochemical and optical properties of water-soluble xanthophyll antioxidants: aggregation vs complexation.

    PubMed

    Polyakov, Nikolay E; Magyar, Adam; Kispert, Lowell D

    2013-09-01

    Xanthophyll carotenoids can self-assemble in aqueous solution to form J- and H-type aggregates. This feature significantly changes the photophysical and optical properties of these carotenoids, and has an impact on solar energy conversion and light induced oxidative damage. In this study we have applied EPR and optical absorption spectroscopy to investigate how complexation can affect the aggregation ability of the xanthophyll carotenoids zeaxanthin, lutein, and astaxanthin, their photostability, and antioxidant activity. It was shown that complexation with the polysaccharide arabinogalactan (AG) polymer matrix and the triterpene glycoside glycyrrhizin (GA) dimer reduced the aggregation rate but did not inhibit aggregation completely. Moreover, these complexants form inclusion complexes with both monomer and H-aggregates of carotenoids. H-aggregates of carotenoids exhibit higher photostability in aqueous solutions as compared with monomers, but much lower antioxidant activity. It was found that complexation increases the photostability of both monomers and the aggregates of xanthophyll carotenoids. Also their ability to trap hydroperoxyl radicals increases in the presence of GA as the GA forms a donutlike dimer in which the hydrophobic polyene chain of the xanthophylls and their H-aggregates lies protected within the donut hole, permitting the hydrophilic ends to be exposed to the surroundings. PMID:23937515

  14. Characterization of Aerogel's Optical Properties

    NASA Astrophysics Data System (ADS)

    Justen, Abigail; Young, Jonathan

    2013-10-01

    Aerogel is used in the kaon aerogel Cerenkov detector at Jefferson Lab. Kaons are identified by the number of photons created through Cerenkov radiation emitted as the kaon travels through the aerogel. Depending on the refractive index of the aerogel, kaons of different momenta can be detected and distinguished from protons. Therefore, a uniform refractive index in the detector is important to reduce uncertainty in the Cerenkov radiation. We found the refractive index of the aerogel by shining a red construction laser through it and measuring how far the beam refracted. The refractive index of aerogel is also directly related to the density of aerogel. The humidity in the air, if absorbed, could also affect the refractive index. To test the effect of humidity on aerogel we used a humidity controlled environment between 80 and 100 percent on aerogel from Matsushita Electric Works, Ltd, Japan Fine Ceramic Center, and Novosibirsk. Finally, we tested the transmittance of aerogel tiles with a UV/Vis photospectrometer to find the correlation between transmittance and the tile's properties. Tiles with the highest transmittance will allow for the most accurate count of the photons produced through Cerenkov radiation. The results from these experiments will be presented. Supported in Part by NSF Grant 1019521 and 1039446.

  15. Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC.

    PubMed Central

    Agyare, Christian; Dwobeng, Anita Serwaa; Agyepong, Nicholas; Boakye, Yaw Duah; Mensah, Kwesi Boadu; Ayande, Patrick George; Adarkwa-Yiadom, Martin

    2013-01-01

    Microbial infections of various types of wounds are a challenge to the treatment of wounds and wound healing. The study was to investigate antimicrobial and antioxidant properties of methanol leaf and stem bark extracts of Kigelia africana and methanol leaf and root extracts of Strophanthus hispidus and also to determine wound healing properties of the extracts. The antimicrobial activities of the methanol extracts were determined against two Gram-positive and two Gram-negative bacteria and a fungus using agar diffusion and micro-dilution methods. The antioxidant activity was determined using 1,1-diphenyl-2-picryl–hydrazyl (DPPH) method. The influence of the extracts on rate of wound closure was investigated using the excision wound model and histopathological investigation of treated and untreated wound tissues performed. The MICs of leaf extract of K. africana against test organisms were 2.5–7.5?mg/mL and stem bark extract were 2.25–7.5?mg/mL. The leaf extract of S. hispidus had MIC range of 2.5–7.5?mg/mL and 2.5–10?mg/mL for root extract. The IC50 of leaf and stem bark extracts of K. africana were 56.9 and 13.7??g/mL, respectively and leaf and root of S. hispidus were 49.8 and 45.1??g/mL, respectively. K. africana extracts (7.5% w/w) showed significant (P < 0.05) wound contraction at day 7 with 72% of wound closure whiles significant (P < 0.05) wound contractions were observed on day 11 for stem bark of K. africana, leaf and root extracts of S. hispidus. Wound tissues treated with the extracts showed improved collagenation, re-epitheliazition and rapid granulation formation compared with untreated wound tissues. The extracts were found to contain alkaloids, saponins, tannins, flavonoids, carbohydrates, and sapogenetic glycosides. The HPLC finger-printing of the extracts were developed. The leaf, stem bark and root extracts of K. africana and S. hispidus exhibited antimicrobial, antioxidant, and enhanced wound healing properties and these may justify the medicinal uses of the plants for treatment of microbial infections and wounds. PMID:23662099

  16. Effect of citric acid deamidation on in vitro digestibility and antioxidant properties of wheat gluten.

    PubMed

    Qiu, Chaoying; Sun, Weizheng; Cui, Chun; Zhao, Mouming

    2013-12-01

    The effects of citric acid deamidation on the physiochemical properties of wheat gluten were investigated. In vitro digestion was carried out to determine changes of molecular weight distribution, amino acids composition and antioxidant efficacy of wheat gluten hydrolysates. Results indicated that citric acid deamidation significantly increased gluten solubility and surface hydrophobicity, at a neutral pH. Deamidation induced molecular weight distribution change of gluten with little proteolysis. Results from FTIR indicated that the ?-helix and ?-turn of deamidated gluten increased accompanied by a decrease of the ?-sheet structure. After deamidation, in vitro pepsin digestibility of wheat gluten decreased, while in vitro pancreatin digestibility increased. The oxygen radical absorbance capacity (ORAC) activity of the in vitro digests decreased with increase of deamidation time. The high Lys and total essential AAs amounts in the final digests suggested that the nutritional values of wheat gluten after deamidation might be enhanced. PMID:23871023

  17. Effect of boiling in water of barley and buckwheat groats on the antioxidant properties and dietary fiber composition.

    PubMed

    H??, Marzanna; Dziedzic, Krzysztof; Górecka, Danuta; Dro?d?y?ska, Agnieszka; Gujska, El?bieta

    2014-09-01

    In recent years, there has been an ever-increasing interest in the research of polyphenols obtained from dietary sources, and their antioxidative properties. The purpose of this study was to determine the effect of boiling buckwheat and barley groats on the antioxidant properties and dietary fiber composition. Antioxidative properties were investigated using methyl linoleate model system, by assessing the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and metal chelating activity. The results were compared with butylated hydroxytoluene (BHT). Raw barley and buckwheat groats extracts showed higher DPPH scavenging ability compared to boiled barley and buckwheat groats extracts. Raw barley groats extract exhibited higher antioxidant activity than boiled groats extract in the methyl linoleate emulsion. Higher chelating ability in relation to Fe (II) ions was observed for boiled groats extracts as compared to raw groats extracts. BHT showed small antiradical activity and metal chelating activity, while showing higher antioxidative activity in emulsion system. The analysis of groats extracts using HPLC method showed the presence of rutin, catechin, quercetin, gallic, p-hydroxybenzoic, p-coumaric, o-coumaric, vanillic, sinapic, and ferulic acids. Differences in the content of dietary fiber and its fractions were observed in the examined products. The highest total dietary fiber content was detected in boiled buckwheat groats, while the lowest - in boiled barley groats. The scientific achievements of this research could help consumers to choose those cereal products available on the market, such as barley and buckwheat groats, which are a rich source of antioxidative compounds and dietary fiber. PMID:24938316

  18. Biochemical characterization of the antioxidant system in the scallop Adamussium colbecki, a sentinel organism for monitoring the Antarctic environment

    Microsoft Academic Search

    F. Regoli; G. B. Principato; E. Bertoli; M. Nigro; E. Orlando

    1997-01-01

    The scallop Adamussium colbecki can be profitably used for monitoring Antarctic coastal environments but its utility would be increased if chemical analyses\\u000a of pollutants were integrated with data on their biological effects. Since oxidative stress is a common pathway of toxicity\\u000a induced by xenobiotics, a preliminary biochemical characterization was carried out on the antioxidant system of this species\\u000a and baseline

  19. METABOLIC CONVERSION OF DIETARY FLAVONOIDS ALTERS THEIR ANTI-INFLAMMATORY AND ANTIOXIDANT PROPERTIES

    PubMed Central

    Lotito, Silvina B.; Zhang, Wei-Jian; Yang, Chung S.; Crozier, Alan; Frei, Balz

    2011-01-01

    The notion that dietary flavonoids exert beneficial health effects in humans is often based on in vitro studies using the glycoside or aglycone forms of these flavonoids. However, flavonoids are extensively metabolized in humans, resulting in formation of glucuronide, methyl and sulphate derivatives, which may have different properties than their parent compounds. The goal of this study was to investigate whether different chemical modifications of the same flavonoid molecule affect its biological and antioxidant activities. Hence, we studied the anti-inflammatory effects of several major human metabolites of quercetin and (?)-epigallocatechin-3-O-gallate (EGCG) by assessing their inhibitory effects on tumor necrosis factor ? (TNF?)-induced protein expression of cellular adhesion molecules in human aortic endothelial cells (HAEC). HAEC were incubated with 1–30 ?M quercetin, 3?- or 4?-O-methyl-quercetin, quercetin-3-O-glucuronide and quercetin-3?-sulphate; or 20–100 ?M EGCG, 4?-O-methyl-EGCG and 4?,4?-di-O-methyl-EGCG, prior to co-incubation with 100 U/ml of TNF?. 3?-O-Methyl-quercetin, 4?-O-methyl-quercetin and their parent aglycone compound, quercetin, all effectively inhibited expression of intercellular adhesion molecule-1 (ICAM-1) with IC50 values (concentration required for 50% inhibition) of 8.0, 5.0 and 4.4 ?M, respectively; E-selectin expression was suppressed to a somewhat lesser but still significant degree by all three compounds, whereas vascular cell adhesion molecule-1 (VCAM-1) was not affected. In contrast, quercetin-3-O-glucuronide (20–100 ?M), quercetin-3?-O-sulphate (10–30 ?M) and phenolic acid metabolites of quercetin (20–100 ?M) did not inhibit adhesion molecule expression. 4?,4?-di-O-methyl-EGCG selectively inhibited ICAM-1 expression with an IC50 value of 94 ?M, whereas EGCG (20–60 ?M) and 4?-O-methyl-EGCG (20–100 ?M) had no effect. The inhibitory effects of 3?-O-methyl-quercetin and 4?,4?-di-O-methyl-EGCG on adhesion molecule expression were not related to either inhibition of NF-?B activation or their antioxidant reducing capacity. Our data indicate that flavonoid metabolites have different biological and antioxidant properties than their parent compounds, and suggest that data from in vitro studies using non-metabolites of flavonoids are of limited relevance in vivo. PMID:21571063

  20. Studies on the Food Additive Propyl Gallate: Synthesis, Structural Characterization, and Evaluation of the Antioxidant Activity

    ERIC Educational Resources Information Center

    Garrido, Jorge; Garrido, E. Manuela; Borges, Fernanda

    2012-01-01

    Antioxidants are additives largely used in industry for delaying, retarding, or preventing the development of oxidative deterioration. Propyl gallate (E310) is a phenolic antioxidant extensively used in the food, cosmetics, and pharmaceutical industries. A series of lab experiments have been developed to teach students about the importance and…

  1. Antioxidant Properties of Proanthocyanidins Attenuate Carbon Tetrachloride (CCl4)–Induced Steatosis and Liver Injury in Rats via CYP2E1 Regulation

    PubMed Central

    Zou, Yuan; Zhu, Lei; Wang, Hui-Fang; Dai, Mu-Gen

    2014-01-01

    Abstract Liver steatosis is characterized by lipid dysregulation and fat accumulation in the liver and can lead to oxidative stress in liver. Since proanthocyanidins are present in plant-based foods and have powerful antioxidant properties, we investigated whether proanthocyanidins can prevent oxidative stress and subsequent liver injury. Carbon tetrachloride (CCl4) treatment can cause steatosis in rats that models both alcoholic and non-alcoholic fatty liver disease in humans. We pre-treated rats by oral administration of proanthocyanidins extracted from grape seeds 7 days prior to intragastrically administering CCl4. Proanthocyanidin treatment continued for an additional 2 weeks, after which time liver and serum were harvested, and mediators of liver injury, oxidative stress, and histological features were evaluated. CCl4-treated rats exhibited significant increases in the following parameters as compared to non-treated rats: fat droplets in the liver, liver injury (ALT, AST), and DNA damage (8-OHdG). Additionally, CCl4 treatment decreased antioxidant enzymes SOD, GSH, GPX, and CAT in the liver due to their rapid depletion after battling against oxidative stress. Compared to CCl4-treated rats, treatment with proanthocyanidins effectively suppressed lipid accumulation, liver injury, DNA damage, as well as restored antioxidant enzyme levels. Further investigation revealed that proanthocyanidins treatment also inhibited expression of CYP2E1 in liver, which prevented the initial step of generating free radicals from CCl4. The data presented here show that treatment with orally administered proanthocyanidins prevented liver injury in the CCl4-induced steatosis model, likely through exerting antioxidant actions to suppress oxidative stress and inhibiting the free radical–generating CYP2E1 enzyme. PMID:24712752

  2. Improvement of anti-oxidation properties of carbon fibers by SiC/SiO2 ceramic coating.

    PubMed

    Kim, Bo-Hye; Yang, Kap Seung; Woo, Hee-Gweon; Kim, Su Yeun

    2011-08-01

    To improve the anti-oxidation properties of carbon fibers (CFs), the sol-gel method followed by pyrolysis was used to coat CFs with SiC/SiO2 ceramic coatings. The SiO2 sol-gel coating was performed by dip coating a PAN(polyacrylonitrile)-based stabilized fiber (PSF) in a silica sol prepared by the polycondensation of tetraethylorthosilicate (TEOS) in the presence of an acidic catalyst. The PSF coated with SiO2 sol then underwent heat treatments at high temperatures in an inert atmosphere to deposit the SiC/SiO2 and carbonize the deposited fibers. The surface morphology of the CFs deposited with SiC/SiO2 was characterized using a scanning electron microscope (SEM). The relative oxidation resistance of the SiC/SiO2 layer deposited on the CFs was determined by the weight loss due to the use of a thermogravimetric analyzer (TGA) under flowing air, and the data were used to calculate the activation energies through an Arrhenius plot. PMID:22103138

  3. Physiochemical and antioxidant properties of roselle-mango juice blends; effects of packaging material, storage temperature and time

    PubMed Central

    Mgaya-Kilima, Beatrice; Remberg, Siv Fagertun; Chove, Bernard Elias; Wicklund, Trude

    2015-01-01

    A study was conducted to determine the effects of packaging materials, seasonality, storage temperature and time on physiochemical and antioxidant properties of roselle-mango juice blends. Roselle extract (20%, 40%, 60%, and 80%) was mixed with mango juice and stored in glass and plastic bottles at 4°C and 28°C. Total soluble solids, pH, titratable acidity, reducing sugar, color, vitamin C, total monomeric anthocyanins, total phenols, and antioxidant activity (FRAP) were evaluated in freshly prepared juice, and after, 2, 4, and 6 months of storage. The results showed that total soluble solids, reducing sugars, and pH increased with storage times under different storage time, irrespective of packaging materials. The acidity, color, total monomeric anthocyanin, vitamin C, total phenols, and antioxidant activity decreased during storage irrespective of storage temperature and packaging material. Loss of anthocyanins, total phenols, and vitamin C content were higher in blends stored at 28°C than 4°C. PMID:25838888

  4. Anti-oxidant properties and polyphenolic profile screening of Vitis vinifera stems and leaves crude extracts grown in Perlis, Malaysia

    NASA Astrophysics Data System (ADS)

    Zakaria, Nursyahda; Zulkifli, Razauden Mohamed; Akhir, Fazrena Nadia Md; Basar, Norazah

    2014-03-01

    Grape has become a fast growing agricultural sector in Malaysia producing between 0.62 kg to 2.03 kg waste per vinestock. This study aims to generate useful information on anti-oxidative properties as well as polyphenolic composition of grapevine waste. Stems and leaves of Vitis vinifera cultivated in Perlis, Malaysia were extracted using methanol, ethyl acetate and petroleum ether. Ethyl acetate stems extract exhibited highest total phenolic content. While in DPPH assay, methanolic stems extract show the highest antioxidant activities. This result indicates that total phenolic content in the extracts may not contribute directly to the antioxidant activities. Thin Layer Chromatograms of all crude extracts exhibited good separation under solvent system petroleum ether-ethyl acetate (2:3) resulted in detection of resveratrol in ethyl acetate stems crude extract.

  5. Multivariate calibration by near infrared spectroscopy for the determination of the vitamin E and the antioxidant properties of quinoa.

    PubMed

    Moncada, Guillermo Wells; González Martín, Ma Inmaculada; Escuredo, Olga; Fischer, Susana; Míguez, Montserrat

    2013-11-15

    Quinoa is a pseudocereal that is grown mainly in the Andes. It is a functional food supplement and ingredient in the preparation of highly nutritious food. In this paper we evaluate the potential of near infrared spectroscopy (NIR) for the determination of vitamin E and antioxidant capacity in the quinoa as total phenol content (TPC), radical scavenging activity by DPPH (2,2-diphenyl-2-picryl-hydrazyl) and cupric reducing antioxidant capacity (CUPRAC) expressed as gallic acid equivalent (GAE). For recording NIR a fiber optic remote reflectance probe applied directly on the quinoa samples without treatment was used. The regression method used was modified partial least squares (MPLS). The multiple correlation coefficients (RSQ) and the standard prediction error corrected (SEP(C)) were for the vitamin E (0.841 and 1.70 mg 100 g(-1)) and for the antioxidants TPC (0.947 and 0.08 mg GAE g(-1)), DPPH radical (0.952 and 0.23 mg GAE g(-1)) and CUPRAC ( 0.623 and 0.21 mg GAE g(-1)), respectively. The prediction capacity of the model developed measured by the ratio performance deviation (RPD) for vitamin E (2.51), antioxidants TPC (4.33), DPPH radical (4.55) and CUPRAC (1.55) indicated that NIRS with a fiber optic probe provides an alternative for the determination of vitamin E and antioxidant properties of the quinoa, with a lower cost, higher speed and results comparable with the chemical methods. PMID:24148374

  6. Nanoscale mechanical properties of in-situ tribofilms generated from ZDDP and F-ZDDP with and without antioxidants.

    PubMed

    Somayaji, Anuradha; Mourhatch, Ramoun; Aswath, Pranesh B

    2007-12-01

    Tribofilms with thickness ranging from 100-200 nm were developed in-situ during wear tests using a zinc dialkyl dithiophosphates (ZDDP) and fluorinated ZDDP (F-ZDDP). The influence of the antioxidant alkylated diphenyl amine on the formation and properties of these tribofilm is examined. Results indicate that the thickness of the tribofilms formed when F-ZDDP is used is always thicker than the tribofilm formed with ZDDP. In addition, in the presence of antioxidants the tribofilm thickness is increased. The hardness of these tribofilms in the absence of the antioxidants is significantly higher at the near surface region (0-30 nm) when compared to the films formed in the presence of antioxidant. Nanoscratch tests conducted to examine the abrasion resistance of the tribofilms also indicate that the tribofilms formed by F-ZDDP are more resistant to scratch compared to films formed by ZDDP. In the presence of antioxidant, tribofilms formed by F-ZDDP are significantly thicker while both films behave in a similar fashion in nanoscratch tests. Transmission electron microscopy of the wear debris formed during the tests were examined and results indicate the nucleation and growth of nanoparticles of Fe3O4 with an approximate size of 5-10 nm embedded within an otherwise amorphous tribofilm. PMID:18283818

  7. Chemical characterization, antioxidant and inhibitory effects of some marine sponges against carbohydrate metabolizing enzymes

    PubMed Central

    2012-01-01

    Background More than 15,000 marine products have been described up to now; Sponges are champion producers, concerning the diversity of products that have been found. Most bioactive compounds from sponges were classified into anti-inflammatory, antitumor, immuno- or neurosurpressive, antiviral, antimalarial, antibiotic, or antifouling. Evaluation of in vitro inhibitory effects of different extracts from four marine sponges versus some antioxidants indices and carbohydrate hydrolyzing enzymes concerned with diabetes mellitus was studied. The chemical characterizations for the extracts of the predominating sponges; SP1 and SP3 were discussed. Methods All chemicals served in the biological study were of analytical grade and purchased from Sigma, Merck and Aldrich. All kits were the products of Biosystems (Spain), Sigma Chemical Company (USA), Biodiagnostic (Egypt). Carbohydrate metabolizing enzymes; α-amylase, α-glucosidase, and β-galactosidase (EC3.2.1.1, EC3.2.1.20, and EC3.2.1.23, respectively) were obtained from Sigma Chemical Company (USA). Results Four marine sponges; Smenospongia (SP1), Callyspongia (SP2), Niphates (SP3), and Stylissa (SP4), were collected from the Red Sea at Egyptian coasts, and taxonomically characterized. The sponges' extracts exhibited diverse inhibitory effects on oxidative stress indices and carbohydrate hydrolyzing enzymes in linear relationships to some extent with concentration of inhibitors (dose dependant). The extracts of sponges (3, 1, and 2) showed, respectively, potent-reducing power. Purification and Chemical characterization of sponge 1 using NMR and mass spectroscopy, recognized the existence of di-isobutyl phthalate (1), di-n-butyl phthalate (2), linoleic acid (3), ?-sitosterol (4), and cholesterol (5). Sponge 3 produced bis-[2-ethyl]-hexyl-phthylester (6) and triglyceride fatty acid ester (7). Conclusion Marine sponges are promising sources for delivering of bioactive compounds. Four marine sponges, collected from Red Sea at Egyptian coasts, were identified as Smenospongia (SP1), Callyspongia (SP2), Niphates (SP3), and Stylissa (SP4). The results demonstrated that different sponges extracts exhibited inhibitory effects on oxidative stress indices and carbohydrate hydrolyzing enzymes in linear relationships to some extent with concentration of inhibitors (dose dependant). The extracts of sponges (3, 1, and 2) showed, respectively, potent-reducing power. Chemical characterizations of sponges SP1 and SP3 were discussed. Based on this study, marine sponges are considered as talented sources for production of diverse and multiple biologically active compounds. PMID:22898269

  8. Putative mechanism for apoptosis-inducing properties of crude saponin isolated from sea cucumber (Holothuria leucospilota) as an antioxidant compound

    PubMed Central

    Soltani, Mozhgan; Parivar, Kazem; Baharara, Javad; Kerachian, Mohammad Amin; Asili, Javad

    2015-01-01

    Objective(s): Marine organisms are known as a potential source of natural products, which contain bioactive substances with therapeutic properties. Sea cucumbers are prominent among marine organisms because of their dietary and therapeutic applications. In addition, they have capacity of synthesizing saponins molecules and other metabolites with therapeutic properties such as antitumor, antimicrobial, anti-inflammatory and antioxidant activities. The aim of this study was to evaluate the antioxidant and pro-apoptotic effects of sea cucumber saponins (SCS) isolated from Holothuria leucospilota species. Materials and Methods: Evaluation of antioxidant activity of SCS was carried out by DPPH (1, 1-diphenyl-2-picrylhydrazyl), ABTS (azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), power reducing and total antioxidant assays. The anti-proliferative effect was studied by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Mechanisms leading to apoptosis were also evaluated by fluorescence microscopy, flow cytometry and real time PCR. Results: The results showed that the DPPH and ABTS activities increased in a dose dependent manner. The reducing capacity enhanced with increasing concentration of the saponin extract (0 to 2 mg/ml). The SCS exhibited moderate total antioxidant activity. Evaluation of anti-proliferative effect revealed that SCS with IC50 of about 6 ?g/ml, can display a good cytotoxic activity in a dose dependent manner. Further apoptosis induction was confirmed by fluorescence microscopy and flow cytometry. Sea cucumber saponin was also found to exert a pro-apoptotic effect by increasing the expression of Bax and decreasing the expression of Bcl2. Conclusion: These results indicate that the SCS may act as a natural antioxidant and antitumor agent. PMID:25810893

  9. Characterization of in vivo antioxidant constituents and dual-standard quality assessment of Danhong injection.

    PubMed

    Liu, Hai-Tao; Wang, Yue-Fei; Olaleye, Olajide; Zhu, Yan; Gao, Xiu-Mei; Kang, Li-Yuan; Zhao, Tao

    2013-05-01

    Antioxidants and oxidative stress play a critical role in cardiovascular diseases. Danhong injection (DHI) is a well prescribed cardiovascular medication in China, but its detailed chemical basis and mechanisms of action remain unknown. To prove the antioxidant activity of DHI, its free radical scavenging capacity (RSC) was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) spectrophotometric assay. The 50% radical scavenging activity value was 1:129.2 mL/mL, against 0.95 mM DPPH. To further identify the antioxidant compounds, modified thin-layer chromatography combined with DPPH bioautography assay was used. Compared with vitamin C, 11 of 16 available compounds displayed strong antioxidant activity, which were also detected in rat serum after intravenous administration of DHI by ultra-performance liquid chromatography-tandem mass spectrometry, except for hydroxysafflor yellow A. Therefore, 10 antioxidants remaining in the blood as key markers, and six other compounds as general markers, were employed to perform the quality control of DHI by ultra-performance liquid chromatography-ultraviolet detection after systematic methodological validation. The analytical results indicate a high correlation (r = 0.9) between the total content of those antioxidants remaining in blood and RSC of DHI among 10 batches. Further, the antioxidant profiling and chemical marker quantification as dual-standard quality assessment was successfully applied to evaluate Danshen and safflower injections. PMID:23233112

  10. Biochemical and molecular characterization of the antioxidative system of Coffea sp. under cold conditions in genotypes with contrasting tolerance.

    PubMed

    Fortunato, Ana S; Lidon, Fernando C; Batista-Santos, Paula; Leitão, António Eduardo; Pais, Isabel P; Ribeiro, Ana I; Ramalho, José Cochicho

    2010-03-15

    Low positive temperature (chilling) is frequently linked to the promotion of oxidative stress conditions, and is of particular importance in the coffee plant due to its severe impact on growth, development, photosynthesis and production. Nevertheless, some acclimation ability has been reported within the Coffea genus, and is possibly related to oxidative stress control. Using an integrated biochemical and molecular approach, the characterization of the antioxidative system of genotypes with different cold acclimation abilities was performed. Experiments were carried out using 1.5-year-old coffee seedlings of Coffea canephora cv. Apoatã, C. arabica cv. Catuaí, C. dewevrei and 2 hybrids, Icatu (C. arabicaxC. canephora) and Piatã (C. dewevreixC. arabica) subjected to a gradual cold treatment and a recovery period. Icatu showed the greatest ability to control oxidative stress, as reflected by the enhancement of several antioxidative components (Cu,Zn-SOD and APX activities; ascorbate, alpha-tocopherol and chlorogenic acids (CGAs) contents) and lower reactive oxygen species contents (H(2)O(2) and OH). Gene expression studies show that GRed, DHAR and class III and IV chitinases might also be involved in the cold acclimation ability of Icatu. Catuaí showed intermediate acclimation ability through the reinforcement of some antioxidative molecules, usually to a lesser extent than that observed in Icatu. On the other hand, C. dewevrei showed the poorest response in terms of antioxidant accumulation, and also showed the greatest increase in OH values. The difference in the triggering of antioxidative traits supports the hypothesis of its importance to cold (and photoinhibition) tolerance in Coffea sp. and could provide a useful probe to identify tolerant genotypes. PMID:19939498

  11. Effect of extrusion cooking on the physicochemical properties, resistant starch, phenolic content and antioxidant capacities of green banana flour.

    PubMed

    Sarawong, Chonthira; Schoenlechner, Regine; Sekiguchi, Ken; Berghofer, Emmerich; Ng, Perry K W

    2014-01-15

    Green banana flour was extruded through a co-rotating twin-screw extruder with constant barrel temperature. The objectives of this study were to determine the effect of extrusion cooking variables (feed moisture, FM, 20% and 50%; screw speed, SS, 200 and 400rpm) and storing of the extruded flours at 4°C for 24h on the physicochemical properties, resistant starch (RS), pasting properties and antioxidant capacities. Extrusion cooking at higher FM and lower SS increased the amylose content, which was expressed in highest RS content. Water adsorption index (WAI) and pasting properties were increased, while water solubility index (WSI), total phenolic content (TPC) and antioxidant activities (FRAP, ABTS(+), DPPH) in free and bound phenolics were decreased compared to the other extruded samples. Storing the extruded flours at 4°C for 24h prior to oven drying was the main factor leading to a further increase in the content of amylose, RS, TPC and WSI values, as well as pasting properties - in particular peak viscosity. Compared to native banana flour, extrusion cooking caused significant changes in all studied properties of the extruded flours, except for soluble DF and antioxidant capacity (ABTS(+) and DPPH) of bound phenolics. PMID:24054209

  12. Effects of ionizing radiation on the antioxidant system of microscopic fungi with radioadaptive properties found in the Chernobyl exclusion zone.

    PubMed

    Tugay, Tatyana I; Zheltonozhskaya, Marina V; Sadovnikov, Leonid V; Tugay, Andrei V; Farfán, Eduardo B

    2011-10-01

    Some microscopic fungi found in the area of the Chernobyl Exclusion Zone appear to have unique radioadaptive properties associated with their capability to respond positively to the effects of ionizing irradiation. On the one hand, this capability can be used potentially in bio-remediation technologies, and on the other hand, it requires additional, more thorough studies to identify its underlying mechanisms. Practically, no data are currently available on mechanisms for implementation of these radioadaptive properties by microscopic fungi. The objective of the completed study was to evaluate the functioning of the antioxidant system of a microscopic fungus as one of potential mechanisms for implementation of its radioadaptive properties. The study was performed using a model system simulating the soil radioactivity in the 5-km zone around the Chernobyl Nuclear Power Plant, with the ratio of the radioactive isotopes matching the radionuclide content in the fuel component of the Chernobyl fallout. The completed study was the first ever performed to identify a comprehensive response of the major components of the antioxidant system of the microscopic fungi to ionizing radiation, resulting in an induced melanin synthesis and increased activity of the known enzymes of antioxidant protection. Their response to ionizing radiation depended on the presence or absence of radioadaptive properties and phase of the fungal growth. Fungi with radioadaptive properties have a much higher susceptibility for inducing synthesis of melanin and antioxidant enzymes than fungi without radioadaptive properties (hereinafter referred to as the reference species or strains), which illustrates the contribution of these processes to "radiophilia" of the fungi. PMID:21878763

  13. Synthesis and characterization of 1,2-bis(2-(5-bromo-2-hydroxybenzilidenamino)-4-chlorophenoxy)ethane and its metal complexes: An experimental, theoretical, electrochemical, antioxidant and antibacterial study

    NASA Astrophysics Data System (ADS)

    Ilhan, Salih; Baykara, Haci; Oztomsuk, Abdussamet; Okumus, Veysi; Levent, Abdulkadir; Salih Seyitoglu, M.; Ozdemir, Sadin

    2014-01-01

    A new Schiff base ligand was synthesized by reaction of 5-bromosalicylaldehyde with 1,2-bis(4-chloro-2-aminophenoxy)ethane. Then the Schiff base complexes were synthesized by the reaction of metal salts and the novel Schiff base. The molar conductivity properties of the complexes were studied and found out that the complexes are nonelectrolytes. The structures of the ligand and its metal complexes were characterized by elemental analysis, FT-IR, UV-VIS, magnetic susceptibility measurements, molar conductivity measurements, and thermal gravimetric analysis. In addition antioxidant, theoretical NMR studies and cyclic voltammetry of the complexes were done. Two methods namely metal chelating activity and diphenylpicrylhydrazyl (DPPH) radical scavenging method were used to determine the antioxidant activity, and antibacterial properties of the compounds were also studied.

  14. Synthesis and characterization of 1,2-bis(2-(5-bromo-2-hydroxybenzilidenamino)-4-chlorophenoxy)ethane and its metal complexes: an experimental, theoretical, electrochemical, antioxidant and antibacterial study.

    PubMed

    Ilhan, Salih; Baykara, Haci; Oztomsuk, Abdussamet; Okumus, Veysi; Levent, Abdulkadir; Salih Seyitoglu, M; Ozdemir, Sadin

    2014-01-24

    A new Schiff base ligand was synthesized by reaction of 5-bromosalicylaldehyde with 1,2-bis(4-chloro-2-aminophenoxy)ethane. Then the Schiff base complexes were synthesized by the reaction of metal salts and the novel Schiff base. The molar conductivity properties of the complexes were studied and found out that the complexes are nonelectrolytes. The structures of the ligand and its metal complexes were characterized by elemental analysis, FT-IR, UV-VIS, magnetic susceptibility measurements, molar conductivity measurements, and thermal gravimetric analysis. In addition antioxidant, theoretical NMR studies and cyclic voltammetry of the complexes were done. Two methods namely metal chelating activity and diphenylpicrylhydrazyl (DPPH) radical scavenging method were used to determine the antioxidant activity, and antibacterial properties of the compounds were also studied. PMID:24096058

  15. Extraction, preliminary characterization and evaluation of in vitro antitumor and antioxidant activities of polysaccharides from Mentha piperita.

    PubMed

    Liu, Xin; Sun, Zhen-Liang; Jia, Ai-Rong; Shi, Ya-Ping; Li, Rui-Hong; Yang, Pei-Ming

    2014-01-01

    This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP). The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM) at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I), and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products. PMID:25226538

  16. Extraction, Preliminary Characterization and Evaluation of in Vitro Antitumor and Antioxidant Activities of Polysaccharides from Mentha piperita

    PubMed Central

    Liu, Xin; Sun, Zhen-Liang; Jia, Ai-Rong; Shi, Ya-Ping; Li, Rui-Hong; Yang, Pei-Ming

    2014-01-01

    This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP). The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM) at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I), and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products. PMID:25226538

  17. Flavonoid antioxidants.

    PubMed

    Rice-Evans, C

    2001-06-01

    In order to ascertain the role of dietary flavonoids as antioxidants in vivo it is necessary to understand the chemical nature of the absorbed forms in the circulation in vivo and how the multiplicity of research findings in vitro reflect the bioactivity of flavonoids in vivo. Only when we gain adequate information on the circulating forms can we begin to understand the targeting to the tissues, whether flavonoids cross the blood-brain barrier, for example, and in what forms. Flavonoids are powerful antioxidants in vitro, but their overall function in vivo has yet to be clarified, whether antioxidant, anti-inflammatory, enzyme inhibitor, enzyme inducer, inhibitor of cell division, or some other role. It should also be emphasised that the reducing properties of flavonoids might contribute to redox regulation in cells, independently of their antioxidant properties, and thus might protect against cell ageing, for example, by working together with the intracellular reductant network. To gain understanding of these issues the factors influencing the absorption of flavonoids in the gastrointestinal tract needs to be established, namely the questions of: de-glycosylation before absorption, conjugation in the small intestine through glucuronidation, sulphation or methylation etc, metabolism and degradation in the colon to smaller phenolic molecules. The forms in which they circulate in vivo will influence their polarity and, thus, their localization and bioactivities in vivo. Finally if antioxidant activities are important, the elucidation of how such properties in vitro relate to the potential for conjugates and metabolites in vivo to act as antioxidants is required. The absorbed flavonoid components might function in the aqueous phase (like vitamin C) or in the lipophilic milieu (as vitamin E) in vivo. This will depend on their polarity properties on uptake, how they are metabolised on absorption, and their resulting structural forms in the circulation. PMID:11375750

  18. Chitosan extracted from mud crab (Scylla olivicea) shells: physicochemical and antioxidant properties.

    PubMed

    Sarbon, N M; Sandanamsamy, S; Kamaruzaman, S F S; Ahmad, F

    2015-07-01

    The aim of this study is to report the yield of extraction, as well as the physicochemical and antioxidant properties of extracted chitosan from mud crabs (S.olivacea) as compared to commercial chitosan. The yield obtained for extracted chitosan was 44.57?±?3.44 % with a moisture and ash content of 9.48?±?0.59 % and 5.97?±?0.90 %, respectively. Commercial chitosan demonstrated a higher degree of deacetylation (58.42?±?2.67 %), water (250?±?9.90 %) and fat (329?±?7.07 %) binding capacity, solubility (73.85 %), viscosity (463.25?±?13.10 %) and also the whiteness value (77.8?±?0.47) compared to the extracted chitosan, which were only 53.42?±?0.88 %, 180?±?0.00 %, 260?±?0.00 %, 53.38 %, 383.9?±?28.43 % and 62.1?±?7.52 %, respectively. The structure of extracted and commercial chitosan was also investigated using Fourier Transform Infrared Spectroscopy (FTIR). In conclusion, the extracted chitosan possessed potential properties similar to the commercial chitosan with high reducing power but low in the scavenging activity on the DPPH and hydroxyl radicals compared to the commercial chitosan. PMID:26139891

  19. Antioxidant capacity and lipid characterization of six Georgia-grown pomegranate cultivars.

    PubMed

    Pande, Garima; Akoh, Casimir C

    2009-10-28

    Six pomegranate (Punica granatum) cultivars were investigated for their antioxidant capacity and lipid profile. Total polyphenols were determined according to the Folin-Ciocalteau method. Major organic acids and phenolic compounds were analyzed by RP-HPLC. Two in vitro antioxidant assays, ferric reducing antioxidant power and Trolox equivalent antioxidant capacity, were used to assess antioxidant capacity. Total lipid was extracted according to the Folch method, and fatty acid methyl esters were determined by GC. Tocopherols and phospholipids were identified and quantified by NP-HPLC using a fluorescence detector for tocopherols and an evaporative light scattering detector for phospholipid analysis. Phytosterols were analyzed by GC. The predominant organic acid was citric acid followed by malic acid. The peel fraction had the highest total hydrolyzable tannins content (4792.3-6894.8 mg/100 g of FW). Overall, the highest antioxidant capacity was found in leaves followed by peel, pulp, and seed. Pomegranate seed had an average lipid content of 19.2% with punicic acid as the predominant fatty acid. Pomegranate seed had high contents of alpha-tocopherol (161.2-170.1 mg/100 g) and gamma-tocopherol (80.2-92.8 mg/100 g). PMID:19743855

  20. Purification and characterization of high antioxidant peptides from duck egg white protein hydrolysates.

    PubMed

    Ren, Yao; Wu, Hui; Li, Xiaofeng; Lai, Furao; Xiao, Xinglong

    2014-10-01

    The hydrolysate from duck egg white protein (DEWP) prepared by "SEEP-Alcalase" at degree of hydrolysis (DH) value of 21% (namely HSA21) exhibited high antioxidant capacity in different oxidation systems. A consecutive chromatographic method was then developed for separation and purification of HSA21, including ion-exchange chromatography, macroporous adsorption resin (MAR) and gel filter chromatography. The final peptides "P21-3-75-B" were obtained with significantly enhanced antioxidant activity (p<0.05). It was further confirmed that the product mainly consisted of five oligopeptides (Mr: 202.1, 294.1, 382.1, 426.3, and 514.4Da). Furthermore, the antioxidant activity of P21-3-75-B kept stable after in vitro digestive simulation. Antioxidant capacity of the purified peptides was closely related to the molecular mass, hydrophobic amino acid residues, acidic amino acid and some antioxidant amino acids. This research provided a valuable route for producing new natural-source peptides with strong antioxidant capacity and high nutritious value for our daily intake. PMID:25181341

  1. MECHANICAL PROPERTY CHARACTERIZATION OF SOL-GEL DERIVED NANOMATERIALS

    E-print Network

    Brinker, C. Jeffrey

    MECHANICAL PROPERTY CHARACTERIZATION OF SOL-GEL DERIVED NANOMATERIALS USING AN ACOUSTIC WAVE;#12;For my Terra........ iii #12;MECHANICAL PROPERTY CHARACTERIZATION OF SOL-GEL DERIVED NANOMATERIALS, New Mexico November 2006 #12;Mechanical Property Characterization of Sol-gel Derived Nanomaterials

  2. Antioxidant and antiglycation properties of Passiflora alata and Passiflora edulis extracts

    Microsoft Academic Search

    Martina Rudnicki; Marcos Roberto de Oliveira; Tiago da Veiga Pereira; Flávio Henrique Reginatto; Felipe Dal-Pizzol; José Cláudio Fonseca Moreira

    2007-01-01

    The leaves of Passiflora alata Dryander and Passiflora edulis Sims, traditionally used in American countries to treat both anxiety and nervousness by folk medicine, are rich in polyphenols, which have been reported as natural antioxidants. In this study, the antioxidant activities of P. edulis and P. alata hydroalcoholic leaf extracts were verified in in vitro and ex vivo assays. P.

  3. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract

    Microsoft Academic Search

    Yunfeng Li; Changjiang Guo; Jijun Yang; Jingyu Wei; Jing Xu; Shuang Cheng

    2006-01-01

    Pomegranate is an important source of bioactive compounds and has been used for folk medicine for many centuries. Pomegranate juice has been demonstrated to be high in antioxidant activity and is effective in the prevention of atherosclerosis. In a previous study, we found that pomegranate peel had the highest antioxidant activity among the peel, pulp and seed fractions of 28

  4. Antioxidant and sensory properties of protein hydrolysate derived from Nile tilapia (Oreochromis niloticus) by one- and two-step hydrolysis.

    PubMed

    Yarnpakdee, Suthasinee; Benjakul, Soottawat; Kristinsson, Hordur G; Kishimura, Hideki

    2015-06-01

    Antioxidant and sensory properties of Nile tilapia protein hydrolysates prepared by one- and two-step hydrolysis using commercial proteases were investigated. Hydrolysates prepared using single protease including Alcalase (HA), Flavourzyme (HF), Protamex (HPr) and papain (HPa) had increases in antioxidant activities as the degree of hydrolysis (DH) increased up to 40 % (P?antioxidant activities. When HA was further hydrolysed by papain, the resulting hydrolysate (HAPa) exhibited the highest antioxidant activities for all assays tested (P?antioxidant properties and reduced bitterness, which could serve as the functional supplement. PMID:26028714

  5. Antibacterial, antioxidant and optical properties of edible starch-chitosan composite film containing Thymus kotschyanus essential oil.

    PubMed

    Mehdizadeh, Tooraj; Tajik, Hossein; Razavi Rohani, Seyed Mehdi; Oromiehie, Abdol Rassol

    2012-01-01

    Thyme Essential oils (EO) with antimicrobial and antioxidant properties are widely used in pharmaceutical, cosmetic, and perfume industry. It is also used for flavoring and preservation of several foods. Nowadays, packaging research is receiving a considerable attention due to the development of eco-friendly materials made from natural polymers such as starch and chitosan. In this study Thymus kotschyanus EO concentrations ranging from 0 to 2.0%, incorporated in starch-chitosan composite (S-CH) film were used. Antimicrobial and antioxidant properties significantly increased with the incorporation of EO (p < 0.05). Incorporating EO, increased total color differences (DE), yellowness index (YI) and whiteness index (WI) which were significantly higher than control and its transparency was reduced. Our results pointed out that the incorporation of Thymus kotschyanus EO as a natural antibacterial agent has potential for using the developed film as an active packaging. PMID:25610564

  6. Antibacterial, antioxidant and optical properties of edible starch-chitosan composite film containing Thymus kotschyanus essential oil

    PubMed Central

    Mehdizadeh, Tooraj; Tajik, Hossein; Razavi Rohani, Seyed Mehdi; Oromiehie, Abdol Rassol

    2012-01-01

    Thyme Essential oils (EO) with antimicrobial and antioxidant properties are widely used in pharmaceutical, cosmetic, and perfume industry. It is also used for flavoring and preservation of several foods. Nowadays, packaging research is receiving a considerable attention due to the development of eco-friendly materials made from natural polymers such as starch and chitosan. In this study Thymus kotschyanus EO concentrations ranging from 0 to 2.0%, incorporated in starch-chitosan composite (S-CH) film were used. Antimicrobial and antioxidant properties significantly increased with the incorporation of EO (p < 0.05). Incorporating EO, increased total color differences (DE), yellowness index (YI) and whiteness index (WI) which were significantly higher than control and its transparency was reduced. Our results pointed out that the incorporation of Thymus kotschyanus EO as a natural antibacterial agent has potential for using the developed film as an active packaging. PMID:25610564

  7. Production, characterization and antioxidant potential of protease from Streptomyces sp. MAB18 using poultry wastes.

    PubMed

    Manivasagan, Panchanathan; Venkatesan, Jayachandran; Sivakumar, Kannan; Kim, Se-Kwon

    2013-01-01

    Poultry waste is an abundant renewable source for the recovery of several value-added metabolites with potential industrial applications. This study describes the production of protease on poultry waste, with the subsequent use of the same poultry waste for the extraction of antioxidants. An extracellular protease-producing strain was isolated from Cuddalore coast, India, and identified as Streptomyces sp. MAB18. Its protease was purified 17.13-fold with 21.62% yield with a specific activity of 2398.36?U/mg and the molecular weight was estimated as 43?kDa. The enzyme was optimally active at pH 8-10 and temperature 50-60 ° C and it was most stable up to pH 12 and 6-12% of NaCl concentration. The enzyme activity was reduced when treated with Hg(2+), Pb(2+), and SDS and stimulated by Fe(2+), Mg(2+), Triton X-100, DMSO (dimethyl sulfoxide), sodium sulphite, and ?-mercaptoethanol. Furthermore, the antioxidant activities of protease were evaluated using in vitro antioxidant assays, such as DPPH radical-scavenging activity, O2 scavenging activity, NO scavenging activity, Fe(2+) chelating activity, and reducing power. The enzyme showed important antioxidant potential with an IC50 value of 78 ± 0.28?mg/mL. Results of the present study indicate that the poultry waste-derived protease may be useful as supplementary protein and antioxidant in the animal feed formulations. PMID:23991418

  8. Purification and characterization of antioxidant peptides from leukocyte extract of Crocodylus siamensis.

    PubMed

    Theansungnoen, Tinnakorn; Yaraksa, Nualyai; Daduang, Sakda; Dhiravisit, Apisak; Thammasirirak, Sompong

    2014-02-01

    Antioxidant peptides were isolated from the leukocyte extract of the Siamese crocodile, Crocodylus siamensis. Crocodile leukocyte was extracted by a combination of methods including freeze-thawing, acetic acid extraction and homogenization. The peptides in the leukocyte extract were purified by anion exchange chromatography and reversed phase-high performance liquid chromatography. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was used to evaluate the antioxidant activity of the elution peaks at each purification step. As a result, there were two purified peptides exhibiting strong antioxidant activity in reducing free radicals on DPPH molecules. The amino acid sequences of these peptides were determined by LC-MS/MS as TDVLGLPAK (912.5 Da) and DPNAALPAGPR (1,148.6 Da), and their IC?? values were 153.4 and 95.7 ?M, respectively. The results of this study therefore indicate that leukocyte extract of C. siamensis contains peptides with antioxidant activity which could be used as a novel antioxidant. PMID:24374428

  9. NMR CHARACTERIZATIONS OF PROPERTIES OF HETEROGENEOUS MEDIA

    SciTech Connect

    C.T. Philip Chang; Changho Choi; Jeromy T. Hollenshead; Rudi Michalak; Jack Phan; Ramon Saavedra; John C. Slattery; Jinsoo Uh; Randi Valestrand; A. Ted Watson; Song Xue

    2005-01-01

    A critical and long-standing need within the petroleum industry is the specification of suitable petrophysical properties for mathematical simulation of fluid flow in petroleum reservoirs (i.e., reservoir characterization). The development of accurate reservoir characterizations is extremely challenging. Property variations may be described on many scales, and the information available from measurements reflect different scales. In fact, experiments on laboratory core samples, well-log data, well-test data, and reservoir-production data all represent information potentially valuable to reservoir characterization, yet they all reflect information about spatial variations of properties at different scales. Nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI) provide enormous potential for developing new descriptions and understandings of heterogeneous media. NMR has the rare capability to probe permeable media non-invasively, with spatial resolution, and it provides unique information about molecular motions and interactions that are sensitive to morphology. NMR well-logging provides the best opportunity ever to resolve permeability distributions within petroleum reservoirs. We develop MRI methods to determine, for the first time, spatially resolved distributions of porosity and permeability within permeable media samples that approach the intrinsic scale: the finest resolution of these macroscopic properties possible. To our knowledge, this is the first time that the permeability is actually resolved at a scale smaller than the sample. In order to do this, we have developed a robust method to determine of relaxation distributions from NMR experiments and a novel implementation and analysis of MRI experiments to determine the amount of fluid corresponding to imaging regions, which are in turn used to determine porosity and saturation distributions. We have developed a novel MRI experiment to determine velocity distributions within flowing experiments, and developed methodology using that data to determine spatially resolved permeability distributions. We investigate the use of intrinsic properties for developing improved correlations for predicting permeability from NMR well-logging data and for obtaining more accurate estimates of multiphase flow properties--the relative permeability and capillary pressure--from displacement experiments. We demonstrate the use of MRI measurements of saturation and relaxation for prediction wetting-phase relative permeability for unstable experiments. Finally, we developed an improved method for determining surface relaxivity with NMR experiments, which can provide better descriptions of permeable media microstructures and improved correlations for permeability predictions.

  10. Cognitive enhancement in aged rats after chronic administration of Equisetum arvense L. with demonstrated antioxidant properties in vitro

    Microsoft Academic Search

    Jair Guilherme dos Santos Junior; Fabrício Hoffmann Martins do Monte; Miriam Marcela Blanco; Vanusa Maria do Nascimento Bispo Lanziotti; Flávio Damasseno Maia; Luzia Kalyne de Almeida Leal

    2005-01-01

    The aim of this work was to verify if chronic administration of the hydroalcoholic extract of stems from Equisetum arvense (HAE) reverses the cognitive impairment in aged rats, as well as, evaluates it in vitro antioxidant properties. Chronic administration of HAE at dose of 50 mg\\/kg, i.p., improved both short- and long-term retention of inhibitory avoidance task and ameliorated the

  11. Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity.

    PubMed

    Annamalai, Neelamegam; Veeramuthu Rajeswari, Mayavan; Vijayalakshmi, Shanmugam; Balasubramanian, Thangavel

    2011-12-01

    Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium containing 1% shrimp and crab shell powder (1:1) (w/v), 0.1% K(2)HPO(4), and 0.05% MgSO(4)·7H(2)O. The molecular weight of chitinase was determined by SDS-PAGE to be 36 kDa. The optimum pH, temperature, pH stability, and thermal stability of chitinase were about 8, 37°C, 5-12, and 40-80°C, respectively. The antioxidant activity of A. faecalis AU02 culture supernatant was determined through scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) as 84%, and the antioxidant compound was characterized by TLC and its FT-IR spectrum. The present study proposed that marine wastes can be utilized to generate a high-value-added product and that pharmacological studies can extend its use to the field of medicine. PMID:22131949

  12. Antioxidant activity and characterization of phenolic compounds from bacaba (Oenocarpus bacaba Mart.) fruit by HPLC-DAD-MS(n).

    PubMed

    Abadio Finco, Fernanda D B; Kammerer, Dietmar R; Carle, Reinhold; Tseng, Wen-Hsin; Böser, Sabrina; Graeve, Lutz

    2012-08-01

    The phytochemicals in fruits have been shown to be major bioactive compounds with regard to health benefits. Bacaba (Oenocarpus bacaba Mart.) is a native palm fruit from the Brazilian savannah and Amazon rainforest that plays an important role in the diet of rural communities and is also a source of income for poor people. This paper reports the characterization and analyses of phenolics from bacaba fruit extract. The total phenolic content of bacaba fruit amounted to 1759.27 ± 1.01 mg GAE/100 g, the flavonoid content was 1134.32 ± 0.03 mg CTE/100 g, and the anthocyanin content was 34.69 ± 0.00 mg cyn-3-glc/100 g. The antioxidant activity was evaluated through different assays [ORAC, FRAP, DPPH, TEAC, and cellular antioxidant assay (CAA) assays] and revealed a significant antioxidant capacity for bacaba in comparison to the data available in the literature. The assignment of the phenolic compounds using HPLC-DAD-MS(n) was based on the evaluation of their UV-vis absorption maxima (?(max)) and mass spectral analyses, and 14 compounds were tentatively identified. The results suggest that bacaba fruits are a promising source of phenolics. PMID:22788720

  13. Antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (Raphanus sativus var. niger, Brassicaceae).

    PubMed

    Wang, Lin-Song; Sun, Xiang-Dong; Cao, Ying; Wang, Li; Li, Fang-Jun; Wang, Yi-Fan

    2010-10-01

    The antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. The extract appeared to form a complex with Fe(3+) or Cu(2+). It displayed a concentration-dependant reducing power (1.16OD(700 nm) at a concentration of 4mM) and scavenging effect against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals (with IC(50)=1.74 +/- 0.03 mM). It could promote the cleavage of plasmid DNA with Cu(II)/H(2)O(2) or Cu(II) alone. This DNA damage could be inhibited by horseradish peroxidase, catalase, and EDTA, respectively. The extract also showed growth inhibition of Bel-7402 cells at lower concentration. The results suggested that the formation of reactive oxygen species might be involved in the mechanism of DNA damage. The acylated pelargonidin derivatives extracted from red radish could act as antioxidant and pro-oxidant and their antioxidant and pro-oxidant properties were relative to the reaction conditions. It might provide novel antioxidant and anticarcinogenic agents. PMID:20600520

  14. Assessment of Antioxidant Properties in Fruits of Myrica esculenta: A Popular Wild Edible Species in Indian Himalayan Region

    PubMed Central

    Rawat, Sandeep; Jugran, Arun; Giri, Lalit; Bhatt, Indra D.; Rawal, Ranbeer S.

    2011-01-01

    Crude extract of Myrica esculenta fruits, a wild edible species of Indian Himalayan Region, was evaluated for phenolic compounds and antioxidant properties. Results revealed significant variation in total phenolic and flavonoid contents across populations. Among populations, total phenolic content varied between 1.78 and 2.51?mg gallic acid equivalent/g fresh weight (fw) of fruits and total flavonoids ranged between 1.31 and 1.59?mg quercetin equivalent/g fw. Antioxidant activity determined by 2,2?-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, 1,1-diphenyl-2-picrylhydrazyl radical scavenging and ferric reducing antioxidant power (FRAP) exhibited considerable antioxidant potential and showed significant positive correlation with total phenolic and total flavonoids content. High performance liquid chromatography analysis revealed significant variation (P

  15. Assessment of Antioxidant Properties in Fruits of Myrica esculenta: A Popular Wild Edible Species in Indian Himalayan Region.

    PubMed

    Rawat, Sandeep; Jugran, Arun; Giri, Lalit; Bhatt, Indra D; Rawal, Ranbeer S

    2011-01-01

    Crude extract of Myrica esculenta fruits, a wild edible species of Indian Himalayan Region, was evaluated for phenolic compounds and antioxidant properties. Results revealed significant variation in total phenolic and flavonoid contents across populations. Among populations, total phenolic content varied between 1.78 and 2.51?mg gallic acid equivalent/g fresh weight (fw) of fruits and total flavonoids ranged between 1.31 and 1.59?mg quercetin equivalent/g fw. Antioxidant activity determined by 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, 1,1-diphenyl-2-picrylhydrazyl radical scavenging and ferric reducing antioxidant power (FRAP) exhibited considerable antioxidant potential and showed significant positive correlation with total phenolic and total flavonoids content. High performance liquid chromatography analysis revealed significant variation (P

  16. Evaluation of Anticancer, Antioxidant, and Possible Anti-inflammatory Properties of Selected Medicinal Plants Used in Indian Traditional Medication

    PubMed Central

    Shaikh, Rafik; Pund, Mahesh; Dawane, Ashwini; Iliyas, Sayyed

    2014-01-01

    The present study was carried out to evaluate the anticancer, antioxidant, and possible anti-inflammatory properties of diverse medicinal plants frequently used in Indian traditional medication. The selected botanicals such as Soymida fembrifuga (Roxb.) A. Juss. (Miliaceae), Tinospora cordifolia (Willd.) Miers. (Menispermaceae), Lavandula bipinnata (L.) O. Ktze. (Lamiaceae), and Helicteres isora L. (Sterculiaceae) extracted in different solvents were evaluated for their in vitro anticancer and antioxidant activities. The results obtained indicate that H. isora has potent cytotoxic activity toward the selected cancer cells such as HeLa-B75 (34.21 ± 0.24%), HL-60 (30.25 ± 1.36%), HEP-3B (25.36 ± 1.78%), and PN-15 (29.21 ± 0.52%). Interestingly, the selected botanicals selectively inhibited cyclooxygenase-2 (COX-2) more than (COX-1), which are the key enzymes implicated in inflammation. COX-2 inhibition was observed to be in the range of 19.66-49.52% as compared to COX-1 inhibition (3.93-19.61%). The results of the antioxidant study revealed that the selected plants were found to be effective 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH), and superoxide radical (SOR) scavenging agents. High-performance thin layer chromatography (HPTLC) fingerprint of flavonoids was used as a measure of quality control of the selected plant samples. The results of the present findings strengthen the potential of the selected plants as a resource for the discovery of novel anticancer, anti-inflammatory, and antioxidant agents. PMID:25379467

  17. Evaluation of Anticancer, Antioxidant, and Possible Anti-inflammatory Properties of Selected Medicinal Plants Used in Indian Traditional Medication.

    PubMed

    Shaikh, Rafik; Pund, Mahesh; Dawane, Ashwini; Iliyas, Sayyed

    2014-10-01

    The present study was carried out to evaluate the anticancer, antioxidant, and possible anti-inflammatory properties of diverse medicinal plants frequently used in Indian traditional medication. The selected botanicals such as Soymida fembrifuga (Roxb.) A. Juss. (Miliaceae), Tinospora cordifolia (Willd.) Miers. (Menispermaceae), Lavandula bipinnata (L.) O. Ktze. (Lamiaceae), and Helicteres isora L. (Sterculiaceae) extracted in different solvents were evaluated for their in vitro anticancer and antioxidant activities. The results obtained indicate that H. isora has potent cytotoxic activity toward the selected cancer cells such as HeLa-B75 (34.21 ± 0.24%), HL-60 (30.25 ± 1.36%), HEP-3B (25.36 ± 1.78%), and PN-15 (29.21 ± 0.52%). Interestingly, the selected botanicals selectively inhibited cyclooxygenase-2 (COX-2) more than (COX-1), which are the key enzymes implicated in inflammation. COX-2 inhibition was observed to be in the range of 19.66-49.52% as compared to COX-1 inhibition (3.93-19.61%). The results of the antioxidant study revealed that the selected plants were found to be effective 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH), and superoxide radical (SOR) scavenging agents. High-performance thin layer chromatography (HPTLC) fingerprint of flavonoids was used as a measure of quality control of the selected plant samples. The results of the present findings strengthen the potential of the selected plants as a resource for the discovery of novel anticancer, anti-inflammatory, and antioxidant agents. PMID:25379467

  18. Effects of molecular weight and ratio of guluronic acid to mannuronic acid on the antioxidant properties of sodium alginate fractions prepared by radiation-induced degradation

    Microsoft Academic Search

    Murat ?en

    2011-01-01

    In this study, the effects of the molecular weight and ratio of guluronic acid (G) to mannuronic acid (M), G\\/M, of some sodium alginate (NaAlg) fractions on their antioxidative properties were investigated. Low-molecular-weight-fractions with various G\\/M were prepared by gamma radiation-induced degradation of NaAlg. Change in their molecular weight was monitored. Antioxidant properties of the fractions with various molecular weight

  19. Antioxidant properties of tuna-skin and bovine-hide gelatin films induced by the addition of oregano and rosemary extracts

    Microsoft Academic Search

    J. Gómez-Estaca; L. Bravo; M. C. Gómez-Guillén; A. Alemán; P. Montero

    2009-01-01

    We have investigated the antioxidant properties (FRAP ferric-reducing ability and ABTS radical-scavenging capacity) as well as the light barrier properties of gelatin-based edible films containing oregano or rosemary aqueous extracts. For comparative purposes both bovine-hide and tuna-skin gelatins were studied. The oregano and rosemary extracts were first characterised by both their total phenolic content and antioxidant activity, with the oregano

  20. The relationship of physicochemical properties to the antioxidative activity of free amino acids in Fenton system.

    PubMed

    Mili?, Sonja; Bogdanovi? Pristov, Jelena; Mutavdži?, Dragosav; Savi?, Aleksandar; Spasi?, Mihajlo; Spasojevi?, Ivan

    2015-04-01

    Herein we compared antioxidative activities (AA) of 25 free L-amino acids (FAA) against Fenton system-mediated hydroxyl radical (HO(•)) production in aqueous solution, and examined the relation between AA and a set of physicochemical properties. The rank order according to AA was: Trp > norleucine > Phe, Leu > Ile > His >3,4-dihydroxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydroxyproline > ?-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfur-containing FAA generated different secondary reactive products, which were discriminated by the means of electron paramagnetic resonance spin-trapping spectroscopy. AA showed a general positive correlation with hydrophobicity. However, when taken separately, uncharged FAA exhibited strong positive correlation of AA with hydrophobicity whereas charged FAA showed negative or no significant correlation depending on the scale applied. A general strong negative correlation was found between AA and polarity. Steric parameters and hydration numbers correlated positively with AA of nonpolar side-chain FAA. In addition, a decrease of temperature which promotes hydrophobic hydration resulted in increased AA. This implies that HO(•)-provoked oxidation of FAA is strongly affected by hydrophobic hydration. Our findings are important for the understanding of oxidation processes in natural and waste waters. PMID:25764263

  1. Bioactive properties of commercialised pomegranate (Punica granatum) juice: antioxidant, antiproliferative and enzyme inhibiting activities.

    PubMed

    Les, Francisco; Prieto, Jose M; Arbonés-Mainar, Jose Miguel; Valero, Marta Sofía; López, Víctor

    2015-06-10

    Pomegranate juice and related products have long been used either in traditional medicine or as nutritional supplements claiming beneficial effects. Although there are several studies on this food plant, only a few studies have been performed with pomegranate juice or marketed products. The aim of this work is to evaluate the antioxidant effects of pomegranate juice on cellular models using hydrogen peroxide as an oxidizing agent or DPPH and superoxide radicals in cell free systems. The antiproliferative effects of the juice were measured on HeLa and PC-3 cells by the MTT assay and pharmacologically relevant enzymes (cyclooxygenases, xanthine oxidase, acetylcholinesterase and monoamine oxidase A) were selected for enzymatic inhibition assays. Pomegranate juice showed significant protective effects against hydrogen peroxide induced toxicity in the Artemia salina and HepG2 models; these effects may be attributed to radical scavenging properties of pomegranate as the juice was able to reduce DPPH and superoxide radicals. Moderate antiproliferative activities in HeLa and PC-3 cancer cells were observed. However, pomegranate juice was also able to inhibit COX-2 and MAO-A enzymes. This study reveals some mechanisms by which pomegranate juice may have interesting and beneficial effects in human health. PMID:26030005

  2. Variations of antioxidant properties and NO scavenging abilities during fermentation of tea.

    PubMed

    Xu, Yang; Zhao, Hang; Zhang, Min; Li, Chun-Jie; Lin, Xue-Zhen; Sheng, Jun; Shi, Wei

    2011-01-01

    Tea is known as one of the most popular beverages in the world, which is believed to be beneficial for health. The main components in tea will change a lot depending on the different processes of fermentation, and thus the effects of different teas on human health may differ. The aim of this study is to explore the varied abilities of reactive oxygen species (ROS) and nitric oxide (NO) scavenging during the fermentation of tea. In this study, we conducted the in vitro experiments which involved some reaction systems indicating the abilities of scavenging ROS and NO. We also investigated the effects of tea and their components (catechins, theabrownins, caffeine) on the intracellular levels of ROS and NO, using Raw 264.7 cells as the model. We found that regardless of whether it was out of cell system or in Raw 264.7 cells, the abilities of scavenging ROS would decrease during the fermentation of tea. Further, the post-fermented pu-erh tea showed the best effect on inhibiting the lipopolysaccharide (LPS)-induced production of NO. These findings indicated that the fermentation process caused a change of the components which might be due to the changes of their antioxidant properties and NO scavenging abilities. PMID:21845097

  3. Antioxidant properties of Viburnum opulus and Viburnum lantana growing in Turkey.

    PubMed

    Altun, Mehmet Levent; Cito?lu, Gülcin Saltan; Yilmaz, Betül Sever; Coban, Tülay

    2008-05-01

    In the present study, antioxidant properties of the water extracts of different parts of Viburnum opulus and Viburnum lantana (Caprifoliaceae) were investigated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and superoxide anion scavenging methods. The extracts were prepared from the fruits, branches, and leaves of V lantana and V opulus species. The branch extracts of V lantana and V opulus inhibited superoxide anion in a concentration-dependent manner. Compared with t-tocopherol, the fruit extract of V lantana did not show any scavenging effect on superoxide anion formation. V lantana leaf extracts, however, showed a moderate scavenging effect on superoxide anion formation, whereas V lantana branch extracts showed a strong scavenging effect (IC50 = 3.1 mg/ml) on superoxide anion in higher concentration. On the other hand, all extracts exhibited a scavenging effect on the DPPH radical with various potencies. When compared with butylated hydroxytoluene, V opulus branch and V lantana leaf extracts, as well as V lantana branch, V opulus fruit and V lantana fruit extracts, showed strong DPPH radical scavenging activity with IC50 values of 0.014, 0.035, 0.052, 0.057 and 0.085 mg/ml, respectively. PMID:17852468

  4. Variations of Antioxidant Properties and NO Scavenging Abilities during Fermentation of Tea

    PubMed Central

    Xu, Yang; Zhao, Hang; Zhang, Min; Li, Chun-Jie; Lin, Xue-Zhen; Sheng, Jun; Shi, Wei

    2011-01-01

    Tea is known as one of the most popular beverages in the world, which is believed to be beneficial for health. The main components in tea will change a lot depending on the different processes of fermentation, and thus the effects of different teas on human health may differ. The aim of this study is to explore the varied abilities of reactive oxygen species (ROS) and nitric oxide (NO) scavenging during the fermentation of tea. In this study, we conducted the in vitro experiments which involved some reaction systems indicating the abilities of scavenging ROS and NO. We also investigated the effects of tea and their components (catechins, theabrownins, caffeine) on the intracellular levels of ROS and NO, using Raw 264.7 cells as the model. We found that regardless of whether it was out of cell system or in Raw 264.7 cells, the abilities of scavenging ROS would decrease during the fermentation of tea. Further, the post-fermented pu-erh tea showed the best effect on inhibiting the lipopolysaccharide (LPS)-induced production of NO. These findings indicated that the fermentation process caused a change of the components which might be due to the changes of their antioxidant properties and NO scavenging abilities. PMID:21845097

  5. Important nutritional constituents, flavour components, antioxidant and antibacterial properties of Pleurotus sajor-caju.

    PubMed

    Gogavekar, Shweta S; Rokade, Shilpa A; Ranveer, Rahul C; Ghosh, Jai S; Kalyani, Dayanand C; Sahoo, Akshaya K

    2014-08-01

    Oyster mushroom (Pleurotus sajor-caju) cultivated in the laboratory was studied for nutritional constituents, flavor components, antioxidant and antibacterial properties. Nutritional constituents estimated per 100 g dry weight (d.w.) include protein (29.3 g), carbohydrate (62.97 g), crude fat (0.91 g), ash (6.82 g) and crude fiber (12.3 g). Energy value of this mushroom was about 297.5 kcal/100 g?d.w. Major mineral components estimated include Ca, Fe, and Mg with highest level of 505.0, 109.5 and 108.7 mg/100 g respectively. Methanolic extract containing significant amounts of phenols and flavonoids showed free radical scavenging potential and antibacterial activities against various spp. of Gram positive and Gram negative bacteria. Compounds responsible for antibacterial activities analyzed by GC-MS include ?- Sistosterol, Cholestanol, 1,5-Dibenzoylnaphthalene and 1,2-Benzenedicarboxylic acid. Flavor components extracted by hot extraction method were found to be higher in number and concentration than the cold extraction method. The characteristic flavor component of mushroom i.e. 1-Octen-3-ol was better extracted by hot than the cold. PMID:25114338

  6. Characterization, refining and antioxidant activity of saccharides derived from hemicelluloses of wood and rice husks.

    PubMed

    Rivas, Sandra; Conde, Enma; Moure, Andrés; Domínguez, Herminia; Parajó, Juan Carlos

    2013-11-01

    Samples of rice husks, Eucalyptus globulus wood and Pinus pinaster wood (containing arabinoxylan, acetylated glucuronoxylan and acetylated glucomannan as major hemicellulose components, respectively) were subjected to autohydrolysis. The resulting liquid phases, containing mainly hemicellulose-derived saccharides, were refined by physicochemical methods to reduce their contents of monosaccharides and non-saccharide compounds. Raw autohydrolysis liquors and refined concentrates coming from aqueous treatments were assayed for antioxidant activity using the following assays: reducing power (FRAP), DPPH and ABTS radical scavenging activity and protection of ?-carotene-linoleic emulsions from oxidation. The reducing power and radical scavenging capacity of the non refined fractions were comparable to the ones determined for the reference compound butylhydroxytoluene. Hemicellulose concentrated from the different feedstocks and refining protocols showed a dose dependent antioxidant activity in the range of concentrations evaluated. The in vitro antioxidant activity of concentrates correlated with their phenolic content. PMID:23768385

  7. Antioxidant Activity Characterization, Phytochemical Screening, and Proximate Analysis of Cermela Hutan (Phyllanthus gomphocarpus Hook. F) Roots and Leaves

    PubMed Central

    Bahari, Ebby-Anuar; Zaaba, Nur Eleza; Haron, Norhisham; Dasiman, Razif; Amom, Zulkhairi

    2014-01-01

    Background Roots and leaves of the Cermela Hutan (Phyllanthus gomphocarpus Hook. F) plant were studied to determine antioxidant activity, phytochemical compounds, proportion of carbohydrate, crude protein, moisture, ash, fat, total phenolic content (TPC), and total flavonoid content (TFC). Material/Methods Ten percent (10%) aqueous extract from both Phyllanthus gomphocarpus roots (PGR) and leaves (PGL) were used in this study. Antioxidant activity characterization by TPC, TFC, Ferric Reducing Antioxidant Power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and phytochemical screening, as well as proximate analysis from both extracts were analyzed in this study. Results Phyllanthus gomphocarpus roots (PGR) and leaves (PGL) tested positive for flavonoid, saponin, tannins, and terpenoids, but PGR showed negative result for anthraquinones. In average weight of 100.0 g dry sample, the carbohydrates, protein, moisture, ash, fat, and energy content in PGR and PGL were 80.9%, 5.5%, 7.8%, 3.4%, 2.4%, and 367 Kcal/100 g, and 66.5%, 14.8%, 10.7%, 6.5%, 1.5%, and 399 Kcal/100g, respectively. Antioxidant assessments using FRAP and DPPH assay showed that PGL extracts possessed higher antioxidant capacity by reducing the ferric ion-TPTZ complex by 0.14 mg/ml ±0.0018 and higher scavenging activity, 83.83% ±0.54 as compared to PGR, 0.07 mg/ml ±0.0035 for FRAP and 62.87% ±1.33 for DPPH, respectively. The total phenolics content was significantly higher in PGL (208.77 mg GAE/g ±3.79) as compared to PGR (27.53 mg GAE/g ±0.42). However, there was no significant different in the total flavonoid contents for PGR (34.8 mg QE/g ±3.12) and PGL (32.43 mg QE/g ±3.92). Conclusions Further investigations are suggested to isolate and characterize the other active constituents from this plant in combatting diseases. PMID:25381551

  8. The antioxidative properties of Holy basil and Galangal in cooked ground pork.

    PubMed

    Juntachote, T; Berghofer, E; Siebenhandl, S; Bauer, F

    2006-03-01

    The ethanolic extracts from Holy basil and Galangal were examined for their extraction yield, total phenolic content, antioxidant activity against a ?-carotene-linoleic acid emulsion system, DPPH scavenging activity and reducing power. Ethanolic extracts of Holy basil exhibited higher extraction yield, total phenolic content, antioxidant activity, DPPH scavenging activity and reducing power than ethanolic extracts of Galangal. Antioxidative efficiency to inhibit lipid oxidation of dried Holy basil and dried Galangal powder, ethanolic extracts of Galangal and Holy basil and commercial antioxidant mixture in cooked ground pork was investigated during refrigerated storage at 5°C for 14 days. The quantitative measurements of thiobarbituric acid reactive substances (TBARS), conjugated diene, peroxide value (POV) and hexanal content were used as indicators of lipid oxidation. Increased levels of TBARS value, POV and hexanal content were noticed in all meat samples during storage, however most marked in cooked ground pork without antioxidants, whereas conjugated diene tended to decrease with increasing storage time. Ranking of the treatment in order of their antioxidant effectiveness showed that commercial antioxidant mixture (0.3% citric acid+0.5% ascorbic acid+0.02% ?-tocopherol)>dried Galangal powder>dried Holy basil powder>ethanolic extracts of Galangal>ethanolic extracts of Holy basil>control. Furthermore, in cooked ground pork samples a high correlation between TBARS value and hexanal contents was obtained. PMID:22061728

  9. In Vitro Antioxidant Properties Evaluation of 10 Iranian Medicinal Plants by Different Methods

    PubMed Central

    Moein, Soheila; Moein, Mahmoodreza; Khoshnoud, Mohammad Javad; Kalanteri, Tahereh

    2012-01-01

    Background There is an interest in finding new and safe antioxidants from natural sources such as medicinal plants. Objectives The aim of this study was to evaluate the antioxidant activity of ten Iranian medicinal plants extracts. Materials and Methods For antioxidant activity, the radical scavenging activity, reducing power and phenolic contents of ethanol plant extracts were determined. Gallic acid was used as standard reference with well-documented antioxidant activity. Results The highest antioxidant activity in terms of DPPH radical scavenging was found in Verbascum sinuatum L. Var (VS) with an IC50 equal to 263.52 ± 5.981 ?g/ml and Rosa damascena Mill (RD) with and IC50 equal to 287.9 ± 5.675 ?g/ml that are higher than gallic acid (IC50 = 25.32 ± 5.593 ?g/ml). The highest antioxidant activity in terms of ferric reducing capacity was also found in Verbascum sinuatum L. Var extracts (in 85.08 ± 8.66 ?g/ml concentration with absorbance 0.5). Also, this extract contains the highest phenolic compounds (8.53 ± 0.11 mg/g). Conclusion In this study, Verbascum sinuatum L. Var contains the highest level of phenolic compounds may be contribute to higher free radical scavenging activity and reducing power in comparison to the other plant extracts. Therefore this plant is a good candidate as natural antioxidant. PMID:23482923

  10. Growth, characterization, and properties of nanographene.

    PubMed

    Yang, Wei; He, Congli; Zhang, Lianchang; Wang, Yi; Shi, Zhiwen; Cheng, Meng; Xie, Guibai; Wang, Duoming; Yang, Rong; Shi, Dongxia; Zhang, Guangyu

    2012-05-01

    A systematic study on nanographene grown directly on silicon dioxide substrates is reported. The growth is carried out in a remote plasma-enhanced chemical vapor deposition system at a low temperature of around 550 °C with methane gas as the carbon source. Atomic force microscopy is used to characterize the nanographene morphology, and Raman spectroscopy, X-ray photoelectron spectroscopy, and scanning tunneling microscopy are exploited to identify the in-plane sp(2) bonding structures of nanographene samples. Electrical transport properties are measured at various temperatures down to 4 K. Tunneling effects, minimal conductance at the charge-neutral point, sheet resistances, and Dirac point position at different channel lengths are investigated. In addition, nanographene film possesses high transmittance properties, as indicated by transmittance spectra. Nanographene devices are fabricated from rippled structures, and show great promise for strain-gauge sensor applications. PMID:22378609

  11. Assessment of Total Phenolic and Flavonoid Content, Antioxidant Properties, and Yield of Aeroponically and Conventionally Grown Leafy Vegetables and Fruit Crops: A Comparative Study

    PubMed Central

    Chandra, Suman; Khan, Shabana; Avula, Bharathi; Lata, Hemant; Yang, Min Hye; ElSohly, Mahmoud A.; Khan, Ikhlas A.

    2014-01-01

    A comparison of the product yield, total phenolics, total flavonoids, and antioxidant properties was done in different leafy vegetables/herbs (basil, chard, parsley, and red kale) and fruit crops (bell pepper, cherry tomatoes, cucumber, and squash) grown in aeroponic growing systems (AG) and in the field (FG). An average increase of about 19%, 8%, 65%, 21%, 53%, 35%, 7%, and 50% in the yield was recorded for basil, chard, red kale, parsley, bell pepper, cherry tomatoes, cucumber, and squash, respectively, when grown in aeroponic systems, compared to that grown in the soil. Antioxidant properties of AG and FG crops were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DDPH) and cellular antioxidant (CAA) assays. In general, the study shows that the plants grown in the aeroponic system had a higher yield and comparable phenolics, flavonoids, and antioxidant properties as compared to those grown in the soil. PMID:24782905

  12. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study.

    PubMed

    Chandra, Suman; Khan, Shabana; Avula, Bharathi; Lata, Hemant; Yang, Min Hye; Elsohly, Mahmoud A; Khan, Ikhlas A

    2014-01-01

    A comparison of the product yield, total phenolics, total flavonoids, and antioxidant properties was done in different leafy vegetables/herbs (basil, chard, parsley, and red kale) and fruit crops (bell pepper, cherry tomatoes, cucumber, and squash) grown in aeroponic growing systems (AG) and in the field (FG). An average increase of about 19%, 8%, 65%, 21%, 53%, 35%, 7%, and 50% in the yield was recorded for basil, chard, red kale, parsley, bell pepper, cherry tomatoes, cucumber, and squash, respectively, when grown in aeroponic systems, compared to that grown in the soil. Antioxidant properties of AG and FG crops were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DDPH) and cellular antioxidant (CAA) assays. In general, the study shows that the plants grown in the aeroponic system had a higher yield and comparable phenolics, flavonoids, and antioxidant properties as compared to those grown in the soil. PMID:24782905

  13. Pine Bark and Green Tea Concentrated Extracts: Antioxidant Activity and Comprehensive Characterization of Bioactive Compounds by HPLC–ESI-QTOF-MS

    PubMed Central

    Cádiz-Gurrea, María de la Luz; Fernández-Arroyo, Salvador; Segura-Carretero, Antonio

    2014-01-01

    The consumption of polyphenols has frequently been associated with low incidence of degenerative diseases. Most of these natural antioxidants come from fruits, vegetables, spices, grains and herbs. For this reason, there has been increasing interest in identifying plant extract compounds. Polymeric tannins and monomeric flavonoids, such as catechin and epicatechin, in pine bark and green tea extracts could be responsible for the higher antioxidant activities of these extracts. The aim of the present study was to characterize the phenolic compounds in pine bark and green tea concentrated extracts using high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC–ESI-QTOF-MS). A total of 37 and 35 compounds from pine bark and green tea extracts, respectively, were identified as belonging to various structural classes, mainly flavan-3-ol and its derivatives (including procyanidins). The antioxidant capacity of both extracts was evaluated by three complementary antioxidant activity methods: Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). Higher antioxidant activity values by each method were obtained. In addition, total polyphenol and flavan-3-ol contents, which were determined by Folin–Ciocalteu and vanillin assays, respectively, exhibited higher amounts of gallic acid and (+)-catechin equivalents. PMID:25383680

  14. ANTIOXIDANT AND CYCLOOXYGENASE INHIBITORY PROPERTIES OF INDIVIDUAL FLAVONOIDS ISOLATED FROM BERRIES AND SPINACH

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Dietary polyphenolics are potent antioxidants and their consumption may provide benefits to human health. In addition, certain phytochemicals have shown to be effective inhibitors of cyclooxygenase (COX) enzymes, and may afford protection against inflammatory processes. The objectives were to deter...

  15. Total phenolic content and antioxidative properties of commercial tinctures obtained from some Lamiaceae plants.

    PubMed

    Kowalczyk, Adam; Biskup, Izabela; Fecka, Izabela

    2012-12-01

    The antioxidant level of commercial tinctures from three Lamiaceae plants, Salvia officinalis, Mentha piperita, and Melissa officinalis, have been determined by the Folin-Ciocalteu method, the 2.2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging technique and ABTS assay. Total phenolic content was expressed as GAE (gallic acid equivalent) and ranged from 0.24 to 3.99 mg/mL. Antioxidant activity in the ABTS assay, calculated as TEAC (Trolox equivalent antioxidant capacity), ranged from 23.5 to 35.6 micromol Trolox/mL, while in the DPPH method, the EC50 value ranged from 0.04 to 0.07 mL/assay. Radical scavenging activity was correlated with total phenolic content. Correlations between ABTS and F-C methods, DPPH and F-C methods and ABTS and DPPH methods were calculated. The obtained results can be useful as additional information about the antioxidant activity of galenical preparations. PMID:23413570

  16. Antioxidant and antiplatlet aggregation properties of bark extracts of Garcinia pedunculata and Garcinia cowa.

    PubMed

    Sharma, Anushi; Joseph, G S; Singh, R P

    2014-08-01

    The bark extracts of Garcinia pedunculata and Garcinia cowa, which are abundant in the Northeastern regions of India, were screened for their antioxidant and in vitro antiplatelet aggregating activities. By ?-carotene linoleate model for antioxidant assay, acetone extract of G. pedunculata and hexane extracts of G. cowa exhibited higher antioxidant activity (86.47 and 66.94 % respectively, at 25 ppm) than other extracts. Similar pattern was observed for superoxide radical scavenging method for antioxidant assay. The ethyl acetate extract of G. pedunculata and hexane extract of G. cowa exhibited higher antiplatelet aggregation capacity towards ADP induced platelet aggregation (IC50 0.16 and 0.43 ug, respectively) than other extracts. PMID:25114359

  17. A comparative assessment of antioxidant properties, total phenolic content of einkorn, wheat, barley and their malts.

    PubMed

    Fogarasi, Attila-Levente; Kun, Szilárd; Tankó, Gabriella; Stefanovits-Bányai, Eva; Hegyesné-Vecseri, Beáta

    2015-01-15

    Two einkorn wheat, one barley, three optional winter cultivation wheat and five winter cultivation wheat samples harvested in Hungary in 2011, and their malts were evaluated for their DPPH radical and ABTS radical cation scavenging activity, ferric reduction capacity (FRAP) and total phenolic content (TPC). All einkorn and barley samples exhibited significant antioxidant activities determined by DPPH and ABTS radical scavenging activities. The einkorn samples show higher polyphenol content than the other wheat samples. In all cases the barley sample had the highest antioxidant potential and polyphenol content. The einkorn malts had high DPPH and ABTS radical cation scavenging activities, but the phenolic content was lower against wheat samples. There was significant difference between the antioxidant potential of optional and winter cultivation wheat samples except on ABTS scavenging activities. Einkorn wheat is potentially a new raw material to produce organic beer that might have beneficial effects with its increased antioxidant potential. PMID:25148951

  18. Nutritional characterization and antioxidant capacity of different tissues of Artemisia Annua L.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Evaluation of different tissues of A. annua for their nutritional contents and antioxidant potential demonstrated that the leaves and inflorescence had the highest percentage of protein, crude fat and in vitro digestible fractions but the lowest levels of detergent fibers. These tissues also had th...

  19. Characterization of increased phenolic compounds from fermented Bokbunja ( Rubus coreanus Miq.) and related antioxidant activity

    Microsoft Academic Search

    Hyun Kyoung Ju; Eun Jung Cho; Moon Hee Jang; Yong Yook Lee; Soon Sun Hong; Jeong Hill Park; Sung Won Kwon

    2009-01-01

    This study examined the changes in the phenolic acid-content and antioxidant activity of Rubi Fructus (RF), the fruit of Rubus coreanus Miq., after fermentation with yeast (Saccharomyces cerevisiae). The phenolic acids were fractionated into three forms, free (Fr. A), ester (Fr. B), and insoluble-bound phenolic acids (Fr. C) and quantified by high performance liquid chromatography with a diode array detector

  20. Characterization of N-acetylcysteine and ambroxol in anti-oxidant therapy

    Microsoft Academic Search

    A. Gillissen; D. Nowak

    1998-01-01

    Reactive free oxygen radicals are known to play an important role in the pathogenesis of various lung diseases such as idiopathic pulmonary fibrosis (IPF), adult respiratory distress syndrome (ARDS) or cystic fibrosis (CF). They can originate from endogenous processes or can be part of exogenous exposures (e.g. ozone, cigarette smoke, asbestos fibres). Consequently, therapeutic enhancement of anti-oxidant defence mechanisms in

  1. Antioxidant genes of the emerald ash borer ( Agrilus planipennis): Gene characterization and expression profiles

    Microsoft Academic Search

    Swapna Priya Rajarapu; Praveen Mamidala; Daniel A. Herms; Pierluigi Bonello; Omprakash Mittapalli

    2011-01-01

    Phytophagous insects frequently encounter reactive oxygen species (ROS) from exogenous and endogenous sources. To overcome the effect of ROS, insects have evolved a suite of antioxidant defense genes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione peroxidase (GPX). The emerald ash borer (Agrilus planipennis Fairmaire), an exotic invasive insect pest from Asia has killed millions of

  2. Purification and characterization of antioxidant peptides from enzymatically hydrolyzed chicken egg white.

    PubMed

    Nimalaratne, Chamila; Bandara, Nandika; Wu, Jianping

    2015-12-01

    Egg white is considered as a rich source of high quality proteins with various bioactive peptide fractions. Enzymatic hydrolysis of proteins can be used to release bioactive fractions and different enzymes have different abilities in releasing such bioactive fractions depending on the enzyme's site of activity on a protein. In this study, several proteases were examined for their ability to release antioxidant peptides from hen egg white and protease P was selected based on the antioxidant activity and the digestion yield of the crude protein hydrolysate. A combination of several purification steps including ultrafiltration with low molecular weight cut-off membranes, cation exchange chromatography and reversed phase high performance liquid chromatography was used to purify 'protease P egg white hydrolysate'. Sixteen antioxidant peptides, which were derived from ovalbumin, ovotransferrin and cystatin were isolated from the most active fractions. Amino acid sequences of those peptides were determined using LC-MS/MS. Oxygen radical absorbance capacity (ORAC) values of selected short chain peptides were determined using synthetic peptides. Two peptides AEERYP and DEDTQAMP (Ala-Glu-Glu-Arg-Tyr-Pro and Asp-Glu-Asp-Thr-Gln-Ala-Met-Pro) showed the highest ORAC values. The results from this study indicate that egg white is rich in antioxidant peptides which can be used as a potential source for preparing bioactive ingredients using enzymatic hydrolysis followed by purification techniques. PMID:26041219

  3. Fabrication and characterization of antioxidant pickering emulsions stabilized by zein/chitosan complex particles (ZCPs).

    PubMed

    Wang, Li-Juan; Hu, Ya-Qiong; Yin, Shou-Wei; Yang, Xiao-Quan; Lai, Fu-Rao; Wang, Si-Qi

    2015-03-11

    Lipid peroxidation in oil-in-water (o/w) emulsions leads to rancidity and carcinogen formation. This work attempted to protect lipid droplets of emulsions from peroxidation via manipulation of the emulsions' interface framework using dual-function zein/CH complex particles (ZCPs). ZCP with intermediate wettability was fabricated via a simple antisolvent approach. Pickering emulsions were produced via a simple and inexpensive shear-induced emulsification technique. ZCP was irreversibly anchored at the oil-water interface to form particle-based network architecture therein, producing ultrastable o/w Pickering emulsions (ZCPEs). ZCPE was not labile to lipid oxidation, evidenced by low lipid hydroperoxides and malondialdehyde levels in the emulsions after thermally accelerated storage. The targeted accumulation of curcumin, a model antioxidant, at the interface was achieved using the ZCP as interfacial vehicle, forming antioxidant shells around dispersed droplets. The oxidative stability of ZCPEs was further improved. Interestingly, no detectable hexanal peak appeared in headspace gas chromatography of the Pickering emulsions. The novel interfacial architecture via the combination of steric hindrance from ZCP-based membrane and interfacial cargo of curcumin endowed the emulsions with favorable oxidative stability. This study opens a promising pathway for producing antioxidant emulsions via the combination of Pickering stabilization mechanism and interfacial delivery of antioxidant. PMID:25636210

  4. Biochemical composition and antioxidant properties of Lavandula angustifolia Miller essential oil are shielded by propolis against UV radiations.

    PubMed

    Gismondi, Angelo; Angelo, Gismondi; Canuti, Lorena; Lorena, Canuti; Grispo, Marta; Marta, Grispo; Canini, Antonella; Antonella, Canini

    2014-01-01

    UV radiations are principal causes of skin cancer and aging. Suntan creams were developed to protect epidermis and derma layers against photodegradation and photooxidation. The addition of antioxidant plant extracts (i.e. essential oil) to sunscreens is habitually performed, to increase their UV protective effects and to contrast pro-radical and cytotoxic compounds present in these solutions. According to these observations, in the present work, the alteration of chemical composition and bioactive properties of Lavandula angustifolia Miller essential oil, exposed to UV light, was investigated. UV induced a significant deterioration of lavender oil biochemical profile. Moreover, the antioxidant activity of this solution, in in vitro tests and directly on B16-F10 melanoma cells, greatly decreased after UV treatment. Our results also showed that essential oil was shielded from UV stress by propolis addition. Even after UV treatment, bee glue highly protected lavender oil secondary metabolites from degradation and also preserved their antiradical properties, both in in vitro antioxidant assays and in cell oxidative damage evaluations. This research proposed propolis as highly efficient UV protective and antiradical additive for sunscreens, cosmetics and alimentary or pharmaceutical products containing plant extracts. PMID:24372191

  5. Antioxidant and iron-binding properties of curcumin, capsaicin, and S-allylcysteine reduce oxidative stress in rat brain homogenate.

    PubMed

    Dairam, Amichand; Fogel, Ronen; Daya, Santy; Limson, Janice L

    2008-05-14

    Research demonstrates that antioxidants and metal chelators may be of beneficial use in the treatment of neurodegenerative diseases, such as Alzheimer's disease (AD). This study investigated the antioxidant and metal-binding properties of curcumin, capsaicin, and S-allylcysteine, which are major components found in commonly used dietary spice ingredients turmeric, chilli, and garlic, respectively. The DPPH assay demonstrates that these compounds readily scavenge free radicals. These compounds significantly curtail iron- (Fe2+) and quinolinic acid (QA)-induced lipid peroxidation and potently scavenge the superoxide anion generated by 1 mM cyanide in rat brain homogenate. The ferrozine assay was used to measure the extent of Fe2+ chelation, and electrochemistry was employed to measure the Fe3+ binding activity of curcumin, capsaicin, and S-allylcysteine. Both assays demonstrate that these compounds bind Fe2+ and Fe3+ and prevent the redox cycling of iron, suggesting that this may be an additional method through which these agents reduce Fe2+-induced lipid peroxidation. This study demonstrates the antioxidant and metal-binding properties of these spice ingredients, and it is hereby postulate that these compounds have important implications in the prevention or treatment of neurodegenerative diseases such as AD. PMID:18422331

  6. Comparative evaluation of volatiles, phenolics, sugars, organic acids and antioxidant properties of Sel-42 and Tainung papaya varieties.

    PubMed

    Kelebek, Hasim; Selli, Serkan; Gubbuk, Hamide; Gunes, Esma

    2015-04-15

    The present study was designed to determine the phenolic compounds, organic acids, sugars, aroma profiles and antioxidant properties of Sel-42 and Tainung papayas grown in Turkey. High-performance liquid chromatography/electrospray ionisation tandem mass spectrometry (HPLC-ESI-MS/MS) method was used for the phenolic compounds analysis. Twelve phenolic compounds were identified and quantified in the samples. The total phenolic content of Sel-42 was clearly higher than that of Tainung. Protocatechuic acid-hexoside, gallic acid-deoxyhexoside, ferulic acid and chlorogenic acids were the most abundant phenolics in both cultivars. Aroma composition of papaya was analysed by gas chromatography-mass spectrometry (GC-MS). A total of 46 and 42 aroma compounds, including esters, alcohols, terpenes, lactones, acids, carbonyl compounds, and volatile phenols were identified in the Sel-42 and Tainung, respectively. The significant linear correlation was confirmed between the values for the total phenolic content and antioxidant activity of papaya extracts. PMID:25466106

  7. Cognitive enhancement in aged rats after chronic administration of Equisetum arvense L. with demonstrated antioxidant properties in vitro.

    PubMed

    Guilherme dos Santos, Jair; Hoffmann Martins do Monte, Fabrício; Marcela Blanco, Miriam; Maria do Nascimento Bispo Lanziotti, Vanusa; Damasseno Maia, Flávio; Kalyne de Almeida Leal, Luzia

    2005-07-01

    The aim of this work was to verify if chronic administration of the hydroalcoholic extract of stems from Equisetum arvense (HAE) reverses the cognitive impairment in aged rats, as well as, evaluates it in vitro antioxidant properties. Chronic administration of HAE at dose of 50 mg/kg, i.p., improved both short- and long-term retention of inhibitory avoidance task and ameliorated the cognitive performance in reference and working memory version of the Morris Water Maze. No differences were found between all three groups of young controls, aged controls and EHA-treated animals with regard to the open field and elevated plus maze tests. Indeed, no toxicity manifestations were observed during treatment. In vitro assays revealed that HAE diminished the thiobarbituric acid reactive substances as well as nitrite formation, but did not alter catalase activity. Thus, the cognitive enhancement effects of the HAE may be attributed, at least in part, to it antioxidant action. PMID:15972233

  8. Effects of Athamanta turbith fruit essential oils on CCl4-induced hepatic failure in mice and their antioxidant properties.

    PubMed

    Tomi?, Ana; Bozin, Biljana; Samojlik, Isidora; Milenkovi?, Marina; Mimica-Duki?, Neda; Petrovi?, Silvana

    2010-05-01

    The effects of essential oils isolated from mature fruits of Athamanta turbith ssp. hungarica (Borbás) Tutin and A. turbith ssp. haynaldii (Borbás & Uechtr.) Tutin (Umbelliferae) on some liver biochemical parameters in mice intoxicated with carbon tetrachloride were investigated. Pretreatment with both essential oils extenuated the effects caused by carbon tetrachloride. In order to investigate in vitro antioxidant properties of the oils, three methods were applied: scavenging of both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radicals, as well as a test of inhibition of Fe(2+)/ascorbic-induced lipid peroxidation. Investigated essential oils exhibited modest antioxidant capacity. Therefore, their influence on biochemical parameters in intoxicated animals might be linked to the inhibition of enzymes (cytochrome P450 2E1) involved in metabolic activation of halomethanes. PMID:19927273

  9. Antioxidant and gastric cytoprotective prostaglandins properties of Cassia sieberiana roots bark extract as an anti-ulcerogenic agent

    PubMed Central

    2012-01-01

    Background Cassia sieberiana is a savannah tree with a wide phytotherapeutic application including the use of its roots in the management of various stomach disorders including gastric ulcer, stomach pains and indigestion. The aim of the study is to evaluate the antioxidant, gastric cytoprotective prostaglandins, secretory phospholipase A2, phytochemical and acute toxicity properties of Cassia sieberiana roots bark extract in a bid to justify its phytotherapeutic applications in gastric ulcer. Methods Antioxidant and radical scavenging activities of the roots bark extract of Cassia sieberiana were assayed. Serum secretory phospholipase A2 (sPLA2) concentration and activity and the formation of gastric mucosal prostaglandins E2 (PGE2) and I2 (PGI2) were also assessed. Comparisons between means were performed using analysis of variance (ANOVA) followed by Students Standard Newman-Keuls post hoc analysis to determine statistical significance. P?antioxidant power and can scavenge hydroxyl radicals. The extract also possesses DPPH scavenging activity, can chelate ferrous ion and a dose-dependent protective effect against lipid peroxidation and free radical generation. Prostaglandin studies showed that the roots bark extract dose dependently increased gastric mucosal PGE2 and PGI2 levels and also decreased serum sPLA2 activity. Phytochemical analyses suggest that the roots extract contains polyhydroxyl/phenolic substances. Acute toxicity test showed no sign of toxicity up to a dose level of 2000?mg/kg body weight p.o. Conclusions C. sieberiana roots extract possesses significant antioxidant and gastric cytoprotective prostaglandin properties as well as serum secretory phospholipase A2 inhibitory activity which could be due to its content of polyhydroxy and/or phenolic substances. This may justify its use as an anti-ulcerogenic agent in traditional medicine in West Africa. PMID:22607580

  10. Evidence for anti-inflammatory and antioxidative properties of dried plum polyphenols in macrophage RAW 264.7 cells.

    PubMed

    Hooshmand, Shirin; Kumar, Ajay; Zhang, Ji Yao; Johnson, Sarah A; Chai, Sheau C; Arjmandi, Bahram H

    2015-05-13

    This study presents the anti-inflammatory and antioxidative properties of dried plum (Prunus domestica L.) polyphenols in macrophage RAW 264.7 cells. We hypothesized that dried plum polyphenols have strong anti-inflammatory and antioxidant properties against lipopolysaccharide (LPS)-induced production of the pro-inflammatory markers, nitric oxide (NO) and cyclooxygenase-2 (COX-2), and the lipid peroxidation product, malondialdehyde, in activated macrophage RAW 264.7 cells. To test this hypothesis, macrophage RAW 264.7 cells were stimulated with either 1 ?g ml(-1) (for measurement of NO production) or 1 ng ml(-1) (for measurement of COX-2 expression) of LPS to induce inflammation and were treated with different doses of dried plum polyphenols (0.0, 0.1, 1, 10, 100 and 1000 ?g ml(-1)). Dried plum polyphenols at a dose of 1000 ?g ml(-1) was able to significantly (P < 0.05) reduce NO production by 43%. Additionally, LPS-induced expression of COX-2 was significantly (P < 0.05) reduced by 100 and 1000 ?g ml(-1) dried plum polyphenols. To investigate the antioxidant activity of dried plum polyphenols, macrophage RAW 264.7 cells were stimulated with 100 ?g ml(-1) of FeSO4 + 1 mM ml(-1) of H2O2 to induce lipid peroxidation. Dried plum polyphenols at a dose of 1000 ?g ml(-1) showed a 32% reduction in malondialdehyde production. These findings indicate that dried plum polyphenols are potent anti-inflammatory and antioxidative agents in vitro. PMID:25921826

  11. Antioxidant and anti-dermatophytic properties leaf and stem bark of Xylosma longifolium clos

    PubMed Central

    2013-01-01

    Background The present study was carried out to assess the phytochemical and anti-dermatophytic effect of the leaf and bark extracts of Xylosma longifolium Clos. The leaf and stem bark are used by the indigenous people of Manipur, India for treatment of skin diseases. Methods The leaves and stem barks of Xylosma longifolium were extracted using petroleum ether, chloroform and methanol respectively. The different extracts of each plant parts were tested for antioxidant activity using DPPH assay. The phenolic content was assayed using Folin-Ciocalteu colorimetric method. Each extracts was further analysed by RP-HPLC to quantify some individual flavonoid components. The anti-dermatophytic activity was evaluated both by agar diffusion method and micro wells dilution method against the Microsporum boullardii MTCC 6059, M. canis (MTCC 2820 and MTCC 32700), M. gypseum MTCC 2819, Trichophyton ajelloi MTCC 4878, T. rubrum (MTCC 296 and MTCC 3272). Results The free radical scavenging activity values were ranged from 0.7 to 1.41 mg/ml and 0.6 to 1.23 mg/ml, respectively for leaf and stem bark extracts. The amount of total phenolic contents of the extracts occurred in both leaf and bark in the range of 12 to 56.6 mg GAE/100 g and 16 to 58 mg GAE/100 g respectively. RP-HPLC analysis for flavonoids revealed the presence of two major flavonoid compounds, rutin and catechin. Kaempferol was in trace or absent. Methanol leaf extract showed significant low inhibitory effect against tested fungus Trichophyton ajelloi MTCC 4878 (0.140625 mg/ml) as the most sensitive. These finding suggest that the methanol leaf extract tested contain compounds with antimicrobial properties. Conclusion The results of our study may partially justify the folkloric uses on the plant studied and further provide an evidence that the leaf extract of Xylosma longifolium might be indeed a potential sources of antimicrobial agents. PMID:23819459

  12. The Improvement of The Endogenous Antioxidant Property of Stone Fish (Actinopyga lecanora) Tissue Using Enzymatic Proteolysis

    PubMed Central

    Bordbar, Sara; Ebrahimpour, Afshin; Abdul Hamid, Azizah; Abdul Manap, Mohd Yazid; Anwar, Farooq; Saari, Nazamid

    2013-01-01

    The stone fish (Actinopyga lecanora) ethanolic and methanolic tissue extracts were investigated for total phenolic contents (TPCs) as well as antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging activity and ferric reducing antioxidant power (FRAP) assays. Both extracts showed low amount of phenolics (20.33 to 17.03?mg of gallic acid equivalents/100?g dried sample) and moderate antioxidant activity (39% to 34%??DPPH• radical scavenging activity and 23.95 to 22.30?mmol/100?mL FeSO4 FRAP value). Enzymatic proteolysis was carried out in order to improve the antioxidant activity using six commercially available proteases under their optimum conditions. The results revealed that the highest increase in antioxidant activity up to 85% was obtained for papain-generated proteolysate, followed by alcalase (77%), trypsin (75%), pepsin (68%), bromelain (68%), and flavourzyme (50%) as measured by DPPH• radical scavenging activity, whilst for the FRAP value, the highest increase in the antioxidant activity up to 39.2?mmol/100?mL FeSO4 was obtained for alcalase-generated proteolysate, followed by papain (29.5?mmol/100?mL FeSO4), trypsin (23.2?mmol/100?mL FeSO4), flavourzyme (24.7?mmol/100?mL FeSO4), bromelain (22.9?mmol/100?mL FeSO4), and pepsin (20.8?mmol/100?mL FeSO4). It is obvious that proteolysis of stone fish tissue by proteolytic enzymes can considerably enhance its antioxidant activity. PMID:23586061

  13. Phenolic composition from different loquat (Eriobotrya japonica Lindl.) cultivars grown in China and their antioxidant properties.

    PubMed

    Zhang, Wenna; Zhao, Xiaoyong; Sun, Chongde; Li, Xian; Chen, Kunsong

    2015-01-01

    China is one of the most important centers of diversity for Eriobotrya japonica Lindl. in the world. In this study, seven loquat cultivars grown in China were evaluated for their phenolic compounds and antioxidant activity. Eleven phenolic compounds, i.e., 3-p-coumaroylquinincacid (3-p-CoQA), 5-caffeoylquinic acid (5-CQA), 4-caffeoylquinic acid (4-CQA), 3-caffeoylquinic acid (3-CQA), 5-feruloylquinic acid (5-FQA), quercetin-3-O-galactoside (Q-3-Gal), quercetin-3-O-glucoside (Q-3-Glu), quercetin-3-O-rhamnoside (Q-3-Rha), kaempferol-3-O-galactoside (K-3-Gal), kaempferol-3-O-rhamnoside (K-3-Rha), and kaempferol-3-O-glucoside (K-3-Glu) were identified and quantified in the peel and pulp of the cultivars tested. 3-CQA and 5-CQA were the predominant components in both fruit parts. 2,2-Diphenyl-1-picrylhydrazyl radicals (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS), and ferric reducing antioxidant power (FRAP) assays were used for the antioxidant evaluation. Results showed that peel extracts had higher antioxidant activities than their pulp counterparts in all the cultivars tested, which was correlated with their higher total phenolic contents. The antioxidant potency composite (APC) index showed obvious variations ranging from 64.15 to 100 in the peel and from 59.49 to 97.95 in the pulp of different cultivars, where "Dahongpao" (DHP) and "Luoyangqing" (LYQ) had the highest APC index in the peel and pulp, respectively. Overall, loquat cultivars rich in hydroxycinnamic acids (HCAs) such as 3-p-CoQA, 5-CQA, 4-CQA, 3-CQA and 5-FQA showed relatively higher antioxidant activities, and may be excellent sources of phytochemicals and natural antioxidants. PMID:25569513

  14. [Antioxidants in liver protection].

    PubMed

    Hagymási, Krisztina; Blázovics, Anna

    2004-07-01

    Great importance has been attributed to antioxidants in the prevention and treatment of conditions associated with oxidative stress for many years. At the same time the antioxidants are free radicals themselves, and they can exert prooxidant activity depending on the concentration. They influence the cell redox homeostasis by their prooxidant and antioxidant activity as well. Drugs of chronic liver diseases should be considered, because free radicals are generated during the activity of the monooxygenase system, which affect the tissue oxidised status. Combined antioxidant treatment is more favourable compared with monotherapy, because antioxidants have scavenger-, compartment- and tissue-specificity and they regenerate each other directly, too. Beside their antioxidant property they may also directly regulate many important processes, e.g. cell cycle. We have some favourable results with regard combined antioxidant therapy of liver disease of different etiology. PMID:15320484

  15. Peppermint antioxidants revisited.

    PubMed

    Riachi, Liza G; De Maria, Carlos A B

    2015-06-01

    This review discusses the relationship between the chemical composition and antioxidant property of peppermint tisane and essential oil. Phenolic acids (e.g. rosmarinic and caffeic acids), flavones (e.g. luteolin derivatives) and flavanones (e.g. eriocitrin derivatives) are possibly the major infusion antioxidants. Vitamin antioxidants (e.g. ascorbic acid and carotenoids) are minor contributors to the overall antioxidant potential. Unsaturated terpenes having a cyclohexadiene structure (e.g. terpinene) and minor cyclic oxygenated terpenes (e.g. thymol), may contribute to antioxidant potential whilst acyclic unsaturated oxygenated monoterpenes (e.g. linalool) may act as pro-oxidants in essential oil. Findings on the antioxidant potential of major cyclic oxygenated terpenes (menthol and menthone) are conflicting. Antioxidant behaviour of aqueous/organic solvent extracts and essential oil as well as the effect of environmental stresses on essential oil and phenolic composition are briefly discussed. PMID:25624208

  16. Antioxidant Activity and Chemical Characterization of Essential Oil of Bunium persicum

    Microsoft Academic Search

    Neda Shahsavari; Mohsen Barzegar; Mohammad Ali Sahari; Hasanali Naghdibadi

    2008-01-01

    The search for natural antioxidants, especially of plant origin, has notably increased in recent years. Bunium persicum Boiss. is an economically important medicinal plant growing wild in the dry temperature regions in Iran. In this study, chemical\\u000a constituents of the essential oil of the seed from Bunium persicum Boiss. have been studied by GC\\/MS technique. The major components were caryophyllene

  17. Evaluation of Antioxidant Properties and Mineral Composition of Purslane (Portulaca oleracea L.) at Different Growth Stages

    PubMed Central

    Uddin, Md. Kamal; Juraimi, Abdul Shukor; Ali, Md. Eaqub; Ismail, Mohd Razi

    2012-01-01

    The main objective of this research was to appraise the changes in mineral content and antioxidant attributes of Portulaca oleracea over different growth stages. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) assays. The iodine titration method was used to determine the ascorbic acid content (AAC). DPPH scavenging (IC50) capacity ranged from 1.30 ± 0.04 to 1.71 ± 0.04 mg/mL, while the ascorbic acid equivalent antioxidant activity (AEAC) values were 229.5 ± 7.9 to 319.3 ± 8.7 mg AA/100 g, total phenol content (TPC) varied from 174.5 ± 8.5 to 348.5 ± 7.9 mg GAE/100 g. AAC 60.5 ± 2.1 to 86.5 ± 3.9 mg/100 g and FRAP 1.8 ± 0.1 to 4.3 ± 0.1 mg GAE/g. There was good correlation between the results of TPC and AEAC, and between IC50 and FRAP assays (r2 > 0.9). The concentrations of Ca, Mg, K, Fe and Zn increased with plant maturity. Calcium (Ca) was negatively correlated with sodium (Na) and chloride (Cl), but positively correlated with magnesium (Mg), potassium (K), iron (Fe) and zinc (Zn). Portulaca olerecea cultivars could be used as a source of minerals and antioxidants, especially for functional food and nutraceutical applications. PMID:22949859

  18. Phenolic Composition and Antioxidant Properties of Different Peach [Prunus persica (L.) Batsch] Cultivars in China

    PubMed Central

    Zhao, Xiaoyong; Zhang, Wenna; Yin, Xueren; Su, Mingshen; Sun, Chongde; Li, Xian; Chen, Kunsong

    2015-01-01

    China is an important centre of diversity for Prunus persica. In the present study, 17 Chinese peach cultivars were evaluated for phenolic content and antioxidant activity. Neochlorogenic acid (NCHA), chlorogenic acid (CHA), procyanidin B1 (B1), catechin (CAT), cyanidin-3-O-glucoside (C3G), quercetin-3-O-galactoside (Q3GAL), quercetin-3-O-glucoside (Q3GLU), quercetin-3-O-rutinoside (Q3R), and kaempferol-3-O-rutinoside (K3R) were identified and quantified. CHA and CAT were the predominant components in both the peel and pulp of this fruit. In general, peel extracts showed higher antioxidant activities than the pulp counterparts, consistent with the observed higher phenolic content. The melting peach cultivar “Xinyu” showed the highest antioxidant potency composite (APC) index. The principal component analysis (PCA) of peel phenolics showed a clear distinction between the melting peach and nectarine. Overall, peach cultivars rich in hydroxycinnamates and flavan-3-ols showed relatively higher antioxidant activities and might be excellent sources of phytochemicals and natural antioxidants. PMID:25775157

  19. Alkyl Caffeates Improve the Antioxidant Activity, Antitumor Property and Oxidation Stability of Edible Oil

    PubMed Central

    Wang, Jun; Gu, Shuang-Shuang; Pang, Na; Wang, Fang-Qin; Pang, Fei; Cui, Hong-Sheng; Wu, Xiang-Yang; Wu, Fu-An

    2014-01-01

    Caffeic acid (CA) is distributed widely in nature and possesses strong antioxidant activity. However, CA has lower solubility in non-polar media, which limits its application in fat-soluble food. To increase the lipophilicity of natural antioxidant CA, a series of alkyl caffeates were synthesized and their antioxidant and antitumor activities were investigated. The antioxidant parameters, including the induction period, acid value and unsaturated fatty acid content, of the alkyl caffeates in edible oil were firstly investigated. The results indicated that alkyl caffeates had a lower DPPH IC50 (14–23 µM) compared to CA, dibutyl hydroxy toluene (BHT) and Vitamin C (24–51 µM), and significantly inhibited four human cancer cells (SW620, SW480, SGC7901 and HepG2) with inhibition ratio of 71.4–78.0% by a MTT assay. With regard to the induction period and acid value assays, methyl and butyl caffeates had higher abilities than BHT to restrain the oxidation process and improve the stability of edible oil. The addition of ethyl caffeate to oil allowed maintenance of a higher unsaturated fatty acid methyl ester content (68.53%) at high temperatures. Overall, the alkyl caffeats with short chain length (n<5) assessed better oxidative stability than those with long chain length. To date, this is the first report to the correlations among the antioxidant activity, anticancer activity and oxidative stability of alkyl caffeates. PMID:24760050

  20. Evaluation of Antioxidant Properties and Mineral Composition of Purslane (Portulaca oleracea L.) at Different Growth Stages.

    PubMed

    Uddin, Md Kamal; Juraimi, Abdul Shukor; Ali, Md Eaqub; Ismail, Mohd Razi

    2012-01-01

    The main objective of this research was to appraise the changes in mineral content and antioxidant attributes of Portulaca oleracea over different growth stages. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) assays. The iodine titration method was used to determine the ascorbic acid content (AAC). DPPH scavenging (IC(50)) capacity ranged from 1.30 ± 0.04 to 1.71 ± 0.04 mg/mL, while the ascorbic acid equivalent antioxidant activity (AEAC) values were 229.5 ± 7.9 to 319.3 ± 8.7 mg AA/100 g, total phenol content (TPC) varied from 174.5 ± 8.5 to 348.5 ± 7.9 mg GAE/100 g. AAC 60.5 ± 2.1 to 86.5 ± 3.9 mg/100 g and FRAP 1.8 ± 0.1 to 4.3 ± 0.1 mg GAE/g. There was good correlation between the results of TPC and AEAC, and between IC(50) and FRAP assays (r(2) > 0.9). The concentrations of Ca, Mg, K, Fe and Zn increased with plant maturity. Calcium (Ca) was negatively correlated with sodium (Na) and chloride (Cl), but positively correlated with magnesium (Mg), potassium (K), iron (Fe) and zinc (Zn). Portulaca olerecea cultivars could be used as a source of minerals and antioxidants, especially for functional food and nutraceutical applications. PMID:22949859

  1. Antioxidant properties and total phenolic content of eight Salvia species from Turkey.

    PubMed

    Tosun, Murat; Ercisli, Sezai; Sengul, Memnune; Ozer, Hakan; Polat, Taskin; Ozturk, Erdogan

    2009-01-01

    Methanolic extracts of eight Salvia species, namely S. aethiopis, S. candidissima, S. limbata, S. microstegia, S. nemorosa, S. pachystachys, S. verticillata, and S. virgata, sampled from Eastern Anatolia in Turkey, were screened for their possible antioxidant activities by two complementary test systems, namely DPPH free radical scavenging and beta-carotene/linoleic acid. Total phenolic content of the extracts of Salvia species were performed Folin-Ciocalteu reagent and gallic acid used as standard. A wide variation has been observed among species in terms of antioxidant activity and total phenolic content. In both DPPH and beta-carotene system, the most active plant was Salvia verticillata with a value of IC50=18.3 microg/ml and 75.8%, respectively. This species also has the highest total phenolic content (167.1 mgGAE/g DW). The total amount of phenolics was between 50.3 to 167.1 mgGAE/g DW among species. A positive linear correlation was observed between total phenolic content and antioxidant activity of the extracts. The results suggest that the extract of Salvia species, notably Salvia verticillata with the highest antioxidant activity, can be used as natural antioxidants in the food industry. PMID:19746262

  2. Phenolic composition and antioxidant properties of different peach [Prunus persica (L.) Batsch] cultivars in China.

    PubMed

    Zhao, Xiaoyong; Zhang, Wenna; Yin, Xueren; Su, Mingshen; Sun, Chongde; Li, Xian; Chen, Kunsong

    2015-01-01

    China is an important centre of diversity for Prunus persica. In the present study, 17 Chinese peach cultivars were evaluated for phenolic content and antioxidant activity. Neochlorogenic acid (NCHA), chlorogenic acid (CHA), procyanidin B1 (B1), catechin (CAT), cyanidin-3-O-glucoside (C3G), quercetin-3-O-galactoside (Q3GAL), quercetin-3-O-glucoside (Q3GLU), quercetin-3-O-rutinoside (Q3R), and kaempferol-3-O-rutinoside (K3R) were identified and quantified. CHA and CAT were the predominant components in both the peel and pulp of this fruit. In general, peel extracts showed higher antioxidant activities than the pulp counterparts, consistent with the observed higher phenolic content. The melting peach cultivar "Xinyu" showed the highest antioxidant potency composite (APC) index. The principal component analysis (PCA) of peel phenolics showed a clear distinction between the melting peach and nectarine. Overall, peach cultivars rich in hydroxycinnamates and flavan-3-ols showed relatively higher antioxidant activities and might be excellent sources of phytochemicals and natural antioxidants. PMID:25775157

  3. Characterization of the nutraceutical quality and antioxidant activity in bell pepper in response to grafting.

    PubMed

    Chávez-Mendoza, Celia; Sánchez, Esteban; Carvajal-Millán, Elizabeth; Muñoz-Márquez, Ezequiel; Guevara-Aguilar, Alexandro

    2013-01-01

    The grafting of fruits and vegetables influences fruit quality. The aim of the present work was to assess the effect of the rootstock and the scion on the antioxidant activity and the content in vitamin C, total phenols, lycopene and ?-carotene of bell pepper. The cultivars Fascinato and Jeanette were used as scion and Terrano was used as rootstock. Four harvests in the production cycle of the vegetable were analyzed in a cultivation system under shading nets. The results indicate statistical differences in the content of these bioactive compounds between the varieties, between grafting and not grafting and between sampling dates (p ? 0.05). The vitamin C content, ?-carotene, and antioxidant capacity proved significantly higher in Fascinato than in Janette. On average, grafting increased ?-carotene and vitamin C concentrations and improved the antioxidant capacity, but had no influence on the total phenol or lycopene contents. It is concluded that grafting to the rootstock Terrano improves the nutritional quality of the fruit produced in both varieties of bell pepper studied. PMID:24352022

  4. Characterization and antioxidative activities of polysaccharide in Chinese angelica and its processed products.

    PubMed

    Ji, Peng; Wei, Yanming; Xue, Wenxin; Hua, Yongli; Zhang, Man; Sun, Hongguo; Song, Zhixue; Zhang, Ling; Li, Jinxia; Zhao, Haifu; Zhang, Wenquan

    2014-06-01

    Five polysaccharides from unprocessed Chinese angelica (UCAP), parched one with alcohol (ACAP), soil (SCAP), sesame oil (OCAP) and parched into charred (CCAP) were extracted and purified. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), compositions were analyzed by gas chromatography-mass spectrometry (GC-MS) and antioxidative activities were compared by determining MDA contents and SOD activities of liver tissue in mice damaged with CCl4 after gavage. The results showed that the FT-IR spectra of CCAP and OCAP displayed lower transmittance at around 1050cm(-1) in comparison with that of UCAP. Five polysaccharides were all composed of rhamnose, arabinose, mannose, glucose and galactose. In CCAP, ACAP, OCAP and SCAP, the proportions of arabinose were significantly increased in comparison with that of UCAP. The SOD activities in CCAP and SCAP groups were significantly enhanced, and MDA contents in CCAP, OCAP and SCAP groups were significantly decreased as compared with UCAP group. This indicated that processing could change the structure, composition and enhance antioxidative activity of polysaccharide in Chinese angelica, and CCAP possessed the strongest antioxidative activity. PMID:24680904

  5. Oxidative stress as a risk factor for osteoporosis in elderly Mexicans as characterized by antioxidant enzymes

    PubMed Central

    Sánchez-Rodríguez, Martha A; Ruiz-Ramos, Mirna; Correa-Muñoz, Elsa; Mendoza-Núñez, Víctor Manuel

    2007-01-01

    Background Oxidative stress (OxS) has recently been linked with osteoporosis; however, we do not know the influence of OxS as an independent risk factor for this disease. Methods We conducted a case-control study in 94 subjects ?60 years of age, 50 healthy and 44 with osteoporosis. We measured total antioxidant status, plasma lipid peroxides, antioxidant activity of superoxide dismutase and glutathione peroxidase (GPx), and calculated the SOD/GPx ratio. Bone mineral density was obtained at the peripheral DXA in calcaneus using a portable Norland Apollo Densitometer®. Osteoporosis was considered when subjects had a BMD of 2.5 standard deviations or more below the mean value for young adults. Results GPx antioxidant activity was significantly lower in the group of subjects with osteoporosis in comparison with the group of healthy subjects (p < 0.01); in addition, the SOD/GPx ratio was significantly higher in the group of individuals with osteoporosis (p < 0.05). In logistic regression analysis, we found OxS to be an independent risk factor for osteoporosis (odds ratio [OR] = 2.79; 95% confidence interval [95% CI] = 1.08–7.23; p = 0.034). Conclusion Our findings suggest that OxS is an independent risk factor for osteoporosis linked to increase of SOD/GPx ratio. PMID:18088440

  6. Characterizing the lipid-lowering effects and antioxidant mechanisms of tomato paste.

    PubMed

    Hsu, Yuan-Man; Lai, Ching-Hsiu; Chang, Chih-Yue; Fan, Chien-Teng; Chen, Ching-Tan; Wu, Chieh-Hsi

    2008-03-01

    Lycopene is known to decrease cardiovascular risks. The objective of this study was to investigate the molecular mechanisms of tomato paste containing approximately 0.1% lycopene in regulating lipid metabolism and oxidation. Hamsters fed 3% or 9% tomato paste containing 0.2% cholesterol were subjected to total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), and triglyceride (TG) measurements. Our results showed reduced rates of serum TC and LDL levels due to 9% tomato paste were 14.3% and 11.3% respectively. Concentrations of 3% and 9% of tomato paste after 8 weeks of feeding significantly increased serum HDL levels, by 19.4% and 28.8% respectively. After ingestion of tomato paste for 8 weeks, the plasma malondialdehyde (MDA) levels significantly decreased, by 80.18% and 89.33% respectively, as compared to the cholesterol group. MDA and diene conjugation assays indicated the potent antioxidant activity of the tomato paste. The increased activities of superoxide dismutases (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), further supported the antioxidant effects of the tomato paste. Two dimension-gel electrophoresis (2-DE) analysis revealed that carbonic anhydrase III (CAIII) and adenylate kinase 2 (AK2) may be two important regulators involved in the anti-lipid and antioxidant effects of tomato paste, opening new insight into the nutritional value of tomato in public health promotion. PMID:18323670

  7. Characterization of canthaxanthin isomers isolated from a new soil Dietzia sp. and their antioxidant activities.

    PubMed

    Venugopalan, Vijaylatha; Tripathi, Subhash K; Nahar, Pradip; Saradhi, P Pardha; Das, Rakha H; Gautam, Hemant K

    2013-02-01

    Canthaxanthin (cx) is a potent antioxidant that is chemically synthesized at the industrial scale and has imperative applications in the cosmetic and feed industries. An orange pigmented mesophilic bacterium, designated as K44, was isolated from soil samples of Kargil, India. Biochemical tests, 16S rRNA gene sequencing, and FAME analysis of the bacterium indicated it to belong in the genus Dietzia and is distinct from human isolates. The strain showed 98% 16S rRNA gene sequence homology with Dietzia maris DSM 43102. High-performance liquid chromatography profile of the pigments isolated from K44 showed two major peaks absorbing at 465.3 and 475 nm. The liquid chromatography-mass spectrometry (LC-MS) analysis of both these peaks revealed their m/z to be 564. The molecular weights, LC-MS/MS fragmentation patterns, and lambdamax of these fractions corresponded to all-trans- (475 nm) and 9-cis-(465.3 nm) cx isomers. The antioxidant activities of cis- and trans-cx isomers isolated from this bacterium were found to differ, where the cis-isomer showed higher free radical, superoxide radical, and reactive oxygen species scavenging activities than the alltrans- isomer, suggesting that 9-cis-cx is more effective as an antioxidant than the all-trans-cx. PMID:23412067

  8. A potential antioxidant enzyme belonging to the atypical 2-Cys peroxiredoxin subfamily characterized from rock bream, Oplegnathus fasciatus.

    PubMed

    Saranya Revathy, Kasthuri; Umasuthan, Navaneethaiyer; Whang, Ilson; Jung, Hyung-Bok; Lim, Bong-Soo; Nam, Bo-Hye; Lee, Jehee

    2015-09-01

    Peroxiredoxins (Prxs), a diverse family of antioxidant enzymes, exert their antioxidant function through which different peroxide species are detoxified. This study describes both structural and functional characterization of a mitochondrial Prx identified in rock bream, Oplegnathus fasciatus (RbPrx5). The ORF (573 bp) of RbPrx5 encoded a protein of 190 amino acids (20kDa) containing a putative mitochondrial targeting sequence (residues 1-20) and a thioredoxin-2 motif (residues 31-190) and three conserved Cys residues. Homology assessment and phylogenetic analysis clearly disclosed relatively higher amino acid sequence similarities and a closer evolutionary position of RbPrx5 with those of other teleost homologs. The ORF of RbPrx5 was distributed among six exons as found in other vertebrates, but it possessed an additional exon in its 5'-UTR. In silico examination of RbPrx5 gene's putative promoter region revealed the presence of several cis-elements which may be important in its transcriptional regulation. Constitutive expression of RbPrx5 was detected in eleven tissues with the highest level in the heart. Modulation of RbPrx5 transcription was evidenced from varying mRNA levels in head kidney post in vivo LPS-, poly I:C-, Edwardsiella tarda bacterial- and rock bream iridoviral-challenges. The antioxidant function of RbPrx5 was investigated using recombinant RbPrx5 protein. Results of an in vitro mixed-function oxidase assay demonstrated a dose-dependent inhibition of DNA damage by rRbPrx5. A H2O2 tolerance assay showed that in vivo overexpression of rRbPrx5 increased the bacterial survival under H2O2-mediated oxidative stress condition. These findings provide an overall insight into the structural, expressional and functional aspects of RbPrx5. PMID:25934084

  9. Antioxidant properties of Taraxacum officinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice.

    PubMed

    Colle, Dirleise; Arantes, Leticia Priscilla; Gubert, Priscila; da Luz, Sônia Cristina Almeida; Athayde, Margareth Linde; Teixeira Rocha, João Batista; Soares, Félix Alexandre Antunes

    2012-06-01

    Acetaminophen (APAP) hepatotoxicity has been related to several cases of hepatitis, cirrhosis, and hepatic transplant. As APAP hepatotoxicity is related to reactive oxygen species (ROS) formation and excessive oxidative stress, natural antioxidant compounds have been tested as an alternative therapy to diminish the hepatic dysfunction induced by APAP. Taraxacum officinale Weber (Family Asteraceae), commonly known as dandelion, is used for medicinal purposes because of its choleretic, diuretic, antioxidant, anti-inflammatory, and hepatoprotective properties. This study evaluated the hepatoprotective activity of T. officinale leaf extract against APAP-induced hepatotoxicity. T. officinale was able to decrease thiobarbituric acid-reactive substance levels induced by 200?mg/kg APAP (p.o.), as well as prevent the decrease in sulfhydryl levels caused by APAP treatment. Furthermore, histopathological alterations, as well as the increased levels of serum aspartate and alanine aminotransferases caused by APAP, were prevented by T. officinale (0.1 and 0.5?mg/mL). In addition, T. officinale extract also demonstrated antioxidant activity in vitro, as well as scavenger activity against 2,2-diphenyl-1-picrylhydrazyl and nitric oxide radicals. Our results clearly demonstrate the hepatoprotective effect of T. officinale against the toxicity induced by APAP. The possible mechanisms involved include its scavenger activities against ROS and reactive nitrogen species, which are attributed to the content of phenolic compounds in the extract. PMID:22424457

  10. Use of liposomes as membrane models to evaluate the contribution of drug-membrane interactions to antioxidant properties of etodolac.

    PubMed

    Lúcio, Marlene; Ferreira, Helena; Lima, José L F C; Reis, Salette

    2008-01-01

    This work stresses the need to combine antioxidant assays and drug-membrane interaction studies to describe more accurately the antioxidant profile of non-steroidal anti-inflammatory drugs (NSAIDs). Different experiments performed in liposomes and aqueous solution were compared and used to evaluate the protective effect of etodolac in lipid peroxidation. Lipid peroxidation was induced by the peroxyl radical (ROO*) derived from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and hydroxyl radical (HO*) generated by the Fenton reaction and was assessed by the fluorescence intensity decay of three fluorescence probes with distinct lipophilic properties--fluorescein; hexadecanoyl aminofluorescein (HDAF) and diphenylhexatriene propionic acid (DPHPA). Membrane fluidity changes due to lipid peroxidation were also evaluated by steady-state anisotropy measurements. Interactions of etodolac with lipid bilayers were evaluated by membrane zeta-potential measurements. Results indicate a drug location near the membrane surface and show that etodolac can scavenge the radicals studied but to a variable extent, depending on the assayed media and reactive species. The use of different probes and liposomes as membrane mimetic systems allowed us to conclude that membrane lipoperoxidation is not only related to the scavenging characteristics of the antioxidants, but also to their ability to interact with lipid bilayers. PMID:18796242

  11. Antioxidant and Anti-Inflammatory Properties of Longan (Dimocarpus longan Lour.) Pericarp

    PubMed Central

    Huang, Guan-Jhong; Wang, Bor-Sen; Lin, Wei-Chao; Huang, Shyh-Shyun; Lee, Chao-Ying; Yen, Ming-Tsung; Huang, Ming-Hsing

    2012-01-01

    This study examined the antioxidant and anti-inflammatory activities of the water extract of longan pericarp (WLP). The results showed that WLP exhibited radical scavenging, reducing activity and liposome protection activity. In addition, WLP also inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophages. Further, administration of WLP, in the range of 100–400?mg/kg, showed a concentration-dependent inhibition on paw edema development following carrageenan (Carr) treatment in mice. The anti-inflammatory effects of WLP may be related to NO and tumor necrosis factor (TNF-?) suppression and associated with the increase in the activities of antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase. Overall, the results showed that WLP might serve as a natural antioxidant and inflammatory inhibitor. PMID:22966244

  12. Total phenolics, phenolic acids, isoflavones, and anthocyanins and antioxidant properties of yellow and black soybeans as affected by thermal processing.

    PubMed

    Xu, Baojun; Chang, Sam K C

    2008-08-27

    The effects of boiling and steaming processes on the phenolic components and antioxidant activities of whole yellow (with yellow seed coat and yellow cotyledon) and black (with black seed coat and green cotyledon) soybeans were investigated. As compared to the raw soybeans, all processing methods caused significant (p < 0.05) decreases in total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTC), monomeric anthocyanin content (MAC), DPPH free radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and oxygen radical absorbing capacity (ORAC) in black soybeans. Pressure steaming caused significant (p < 0.05) increases in TPC, CTC, DPPH, FRAP, and ORAC in yellow soybeans. The steaming resulted in a greater retention of TPC, DPPH, FRAP, and ORAC values in both yellow and black soybeans as compared to the boiling treatments. To further investigate the effect of processing on phenolic compounds and elucidate the contribution of these compounds to changes of antioxidant activities, phenolic acids, isoflavones, and anthocyanins were quantitatively determined by HPLC. The pressure steaming treatments caused significant (p < 0.05) increases in gallic acid and 2,3,4-trihydroxybenzoic acid, whereas all treatments caused significant (p < 0.05) decreases in two predominant phenolic acids (chlorogenic acid and trans-cinnamic acid), and total phenolic acids for both yellow and black soybeans. All thermal processing caused significant (p < 0.05) increases in aglucones and beta-glucosides of isoflavones, but caused significant (p < 0.05) decreases in malonylglucosides of isoflavones for both yellow and black soybeans. All thermal processing caused significant (p < 0.05) decreases of cyanidin-3-glucoside and peonidin-3-glucoside in black soybeans. Significant correlations existed between selected phenolic compositions, isoflavone and anthocyanin contents, and antioxidant properties of cooked soybeans. PMID:18680298

  13. [Antiradical and antioxidant properties of nonsteroidal anti-inflammatory agents--derivatives of pyridinecarboxylic acid].

    PubMed

    Gubski?, Iu I; Goriushko, A G; Vistunova, I E; Kurapova, T N; Sachenko, L G; Danilenko, V F

    1999-01-01

    A number of pyridincarboxylic acid derivatives PV-1-4, 7 and emoxypine preparation antioxidative activity in yolk lipoprotein suspension was studied by a method of Fe(2+)-initiated biochemiluminescence. Lipid peroxidation in suspension was effectively inhibited by the studied compounds in various concentration ranges. PV 1, 3, 4, 7 inhibited lipid peroxidation at the concentrations 100-fold, then those of PV 2 and emoxypine. Antiradical activity of the studied compounds was demonstrated by their forming a complex with the stable diphenylpickrylhydrazyl radical. The effect of these compounds as antioxidants is discussed. PMID:10726316

  14. Antioxidant properties and structural analysis of phenolic glucosides from bark of Populus ussuriensis Kom

    Microsoft Academic Search

    Chuan-Ling Si; Jie Xu; Jin-Kyu Kim; Young-Soo Bae; Peng-Tao Liu; Zhong Liu

    2011-01-01

    The methanol (MeOH) extract of Populus ussuriensis Kom. bark was analyzed for antioxidant assessing by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging potential. Among\\u000a fractions using several solvents, the ethyl acetate (EtOAc) soluble fraction, which showed strong antioxidant activity (IC50 2.02 ± 0.01 ?g\\/ml), was further purified by Thin layer chromatography (TLC) guided Sephadex LH-20 column chromatography.\\u000a Three known phenolic glucosides, picein (I), salicortin (II), grandidentatin

  15. Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae

    Microsoft Academic Search

    Mannan Hajimahmoodi; Mohammad Ali Faramarzi; Najmeh Mohammadi; Neda Soltani; Mohammad Reza Oveisi; Nastaran Nafissi-Varcheh

    2010-01-01

    Antioxidant activities of both cells and extracellular substances were evaluated in 12 soil-isolated strains of microalgae\\u000a according to FRAP and DPPH-HPLC assays. Their total phenolic contents were also determined by Folin–Ciocalteu method. Extractions\\u000a were performed with hexane, ethyl acetate, and water. The results of FRAP assay showed that algal cells contained considerable\\u000a amounts of antioxidants from 0.56?±?0.06 to 31.06?±?4.00 µmol Trolox

  16. Differences in antioxidant properties of ginkgo leaves collected from male and female trees.

    PubMed

    Koczka, Noémi; Móczár, Zsuzsanna; Stefanovits-Bányai, Éva; Ombódi, Attila

    2015-03-01

    Total phenolic content and antioxidant capacity (FRAP method) of Ginkgo biloba L. leaves collected from male and female trees were determined and compared. Different water and aqueous ethanolic (water/ethanol 80/20, V/V) extracts were prepared by varying the time of infusing, boiling and steeping in order to determine the effect of the extraction method on the above parameters. Antioxidant activity and phenolic content of ginkgo leaf extracts correlated well with significant correlation coefficients. Slopes of linear regression lines were not statistically different for either sex. PMID:25781709

  17. Properties and antioxidant action of actives cassava starch films incorporated with green tea and palm oil extracts.

    PubMed

    Perazzo, Kátya Karine Nery Carneiro Lins; Conceição, Anderson Carlos de Vasconcelos; dos Santos, Juliana Caribé Pires; Assis, Denilson de Jesus; Souza, Carolina Oliveira; Druzian, Janice Izabel

    2014-01-01

    There is an interest in the development of an antioxidant packaging fully biodegradable to increase the shelf life of food products. An active film from cassava starch bio-based, incorporated with aqueous green tea extract and oil palm colorant was developed packaging. The effects of additives on the film properties were determined by measuring mechanical, barrier and thermal properties using a response surface methodology design experiment. The bio-based films were used to pack butter (maintained for 45 days) under accelerated oxidation conditions. The antioxidant action of the active films was evaluated by analyzing the peroxide index, total carotenoids, and total polyphenol. The same analysis also evaluated unpacked butter, packed in films without additives and butter packed in LDPE films, as controls. The results suggested that incorporation of the antioxidants extracts tensile strength and water vapor barrier properties (15 times lower) compared to control without additives. A lower peroxide index (231.57%), which was significantly different from that of the control (p<0.05), was detected in products packed in film formulations containing average concentration of green tea extracts and high concentration of colorant. However, it was found that the high content of polyphenols in green tea extract can be acted as a pro-oxidant agent, which suggests that the use of high concentration should be avoided as additives for films. These results support the applicability of a green tea extract and oil palm carotenoics colorant in starch films totally biodegradable and the use of these materials in active packaging of the fatty products. PMID:25251437

  18. Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

    PubMed Central

    Sadi, Gökhan; Emsen, Bu?rahan; Kaya, Abdullah; Kocaba?, Aytaç; Ç?nar, Seval; Kartal, Deniz ?rtem

    2015-01-01

    Background: Mushrooms have been valued for their nutritive content and as traditional medicines; several important medicinal properties of mushrooms have been recognized worldwide. Objective: The purpose of this study was to elucidate the cell growth inhibitory potential of four edible mushrooms; Coprinus comatus (O.F. Mull.) Pers. (Agaricaceae), Tricholoma fracticum (Britzelm.) Kreisel (Tricholomataceae), Rhizopogon luteolus Fr. and Nordholm (Rhizopogonaceae), Lentinus tigrinus (Bull.) Fr. (Polyporaceae) on hepatocellular carcinoma (HepG2) cells in conjunction with their antioxidant and antibacterial capacities. Materials and Methods: Five different extracts of edible mushrooms were obtained using water, methanol, acetone, n-hexane and chloroform as solvent systems for cytotoxic, antioxidant and antibacterial properties. Results: C. comatus showed substantial in vitro cytotoxic activity against HepG2 cell lines with all extracts especially with chloroform 50% inhibition (IC50 value of 0.086 mg/ml) and acetone (IC50 value of 0.420 mg/ml). Chloroform extract of C. comatus had maximum amount of ?-carotene (25.94 ?g/mg), total phenolic content (76.32 ?g/mg) and lycopene (12.00 ?g/mg), and n-hexane extract of L. tigrinus had maximum amount of flavonoid (3.67 ?g/mg). While chloroform extract of C. comatus showed the highest 2, 2-diphenyl-1-picrylhydrazyl (DPPH) capturing activity (1.579 mg/ml), the best result for metal chelating activity was obtained from methanolic extract (0.842 mg/ml). Moreover, all tested mushrooms demonstrated antibacterial activity and n-hexane extract of L. tigrinus and acetone extracts of T. fracticum were the most active against tested microorganism. Conclusion: These results indicate that different extracts of investigated mushroom have considerable cytotoxic, antioxidant and antibacterial properties and may be utilized as a promising source of therapeutics. PMID:26109775

  19. Antioxidant and hepatoprotective properties of polyphenol extracts from Telfairia occidentalis (fluted pumpkin) leaves on acetaminophen induced liver damage.

    PubMed

    Nwanna, E E; Oboh, G

    2007-08-15

    In this study the antioxidant and hepatoprotective properties of free and bound polyphenols from Telfairia occidentalis (darkish green leafy vegetable popularly used in soup and folk medicine for the management of many diseases in Nigeria) leaves were compared. Free soluble polyphenols were extracted with 80% acetone, while the bound polyphenols were extracted from the acid and alkaline hydrolyzed residue of the leaf from free soluble polyphenols using ethyl acetate. The total phenol, DPPH free radical scavenging ability and reducing property were determined; subsequently the ability of the extracts to prevent acetaminophen (megadose) induced liver damage in rats were also assessed. Change in serum Glutamate Oxaloacetate Transaminase (GOT), Glutamate Pyruvate Transaminase (GPT), alkaline phosphatase (ALP), albumin, total protein and bilirubin were also determined. The results of the study revealed that the free soluble polyphenols content in the vegetable were significantly higher (p<0.05) than the bound polyphenols. Also, the free soluble polyphenols had a significantly higher antioxidant activity as typified by their higher reducing Power (0.28 OD700) and free radical scavenging ability (83.3%) than the bound polyphenols [reducing power (0.22 OD700), free radical scavenging ability (66.6%)]. Daily intubation of wistar strain albino rat's with 100 mg/mL/day for 7 days caused a significant increase (p<0.05) in serum alkaline phosphatase (ALP), Glutamate Oxaloacetate Transaminase (GOT) and Glutamate Pyruvate Transaminase (GPT), while there was no significant change (p>0.05) in serum bilirubin, albumin, globulin and total proteins in the rats. However, simultaneous intubations of some of the rat with 10 mg or 20 mg mL(-1) of T. occidentalis leaf extract (free soluble or bound polyphenols) along side with the acetaminophen caused a significant decrease (p<0.05) in serum ALP, GOT and GPT (except those intubated with bound polyphenols). Free soluble polyphenols had higher protective effect on the liver than the bound polyphenols; however there action were not dose-dependent. It could be inferred that both soluble free and bound polyphenols extracts of T. occidentails leaf have antioxidant and hepatoprotective properties, however soluble free polyphenols had significantly higher antioxidant and hepatoprotective properties than the bound polyphenols. PMID:19070082

  20. Comparisons on the Functional Properties and Antioxidant Activity of Spray-Dried and Freeze-Dried Egg White Protein Hydrolysate

    Microsoft Academic Search

    Chen Chen; Yu-Jie Chi; Wei Xu

    Freeze-dried and spray-dried egg white protein hydrolysates (FD-EWPH, SD-EWPH) prepared by papain were evaluated for their\\u000a proximate composition, color, molecular weight distribution, physical structures, functional properties, and antioxidant activity.\\u000a FD-EWPH and SD-EWPH had similar proximate composition and molecular weight distribution, but they had different color and\\u000a physical structures. There was no significant difference (P?>?0.05) in solubility among egg white protein

  1. Preparation and Characterization of Antioxidant Nanoparticles Composed of Chitosan and Fucoidan for Antibiotics Delivery

    PubMed Central

    Huang, Yi-Cheng; Li, Rou-Ying

    2014-01-01

    In this study, we developed novel chitosan/fucoidan nanoparticles (CS/F NPs) using a simple polyelectrolyte self-assembly method and evaluated their potential to be antioxidant carriers. As the CS/F weight ratio was 5/1, the CS/F NPs were spherical and exhibited diameters of approximately 230–250 nm, as demonstrated by TEM. These CS/F NPs maintained compactness and stability for 25 day in phosphate-buffered saline (pH 6.0–7.4). The CS/F NPs exhibited highly potent antioxidant effects by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing the concentration of intracellular reactive oxygen species (ROS) and superoxide anion (O2?) in stimulated macrophages. The DPPH scavenging effect of CS/F NPs primarily derives from fucoidan. Furthermore, these CS/F NPs activated no host immune cells into inflammation-mediated cytotoxic conditions induced by IL-6 production and NO generation. The MTT cell viability assay revealed an absence of toxicity in A549 cells after exposure to the formulations containing 0.375 mg NPs/mL to 3 mg NPs/mL. Gentamicin (GM), an antibiotic, was used as a model drug for an in vitro releasing test. The CS/F NPs controlled the release of GM for up to 72 h, with 99% of release. The antioxidant CS/F NPs prepared in this study could thus be effective in delivering antibiotics to the lungs, particularly for airway inflammatory diseases. PMID:25089950

  2. Preparation and characterization of antioxidant nanoparticles composed of chitosan and fucoidan for antibiotics delivery.

    PubMed

    Huang, Yi-Cheng; Li, Rou-Ying

    2014-08-01

    In this study, we developed novel chitosan/fucoidan nanoparticles (CS/F NPs) using a simple polyelectrolyte self-assembly method and evaluated their potential to be antioxidant carriers. As the CS/F weight ratio was 5/1, the CS/F NPs were spherical and exhibited diameters of approximately 230-250 nm, as demonstrated by TEM. These CS/F NPs maintained compactness and stability for 25 day in phosphate-buffered saline (pH 6.0-7.4). The CS/F NPs exhibited highly potent antioxidant effects by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing the concentration of intracellular reactive oxygen species (ROS) and superoxide anion (O2-) in stimulated macrophages. The DPPH scavenging effect of CS/F NPs primarily derives from fucoidan. Furthermore, these CS/F NPs activated no host immune cells into inflammation-mediated cytotoxic conditions induced by IL-6 production and NO generation. The MTT cell viability assay revealed an absence of toxicity in A549 cells after exposure to the formulations containing 0.375 mg NPs/mL to 3 mg NPs/mL. Gentamicin (GM), an antibiotic, was used as a model drug for an in vitro releasing test. The CS/F NPs controlled the release of GM for up to 72 h, with 99% of release. The antioxidant CS/F NPs prepared in this study could thus be effective in delivering antibiotics to the lungs, particularly for airway inflammatory diseases. PMID:25089950

  3. Antioxidant defense properties of Arctic amphipods: comparison between deep-, sublittoral and surface-water species

    Microsoft Academic Search

    L. Camus; B. Gulliksen

    2005-01-01

    The toxic reactive oxygen species (ROS), produced naturally in an oxygenated environment, led to the development of antioxidant defenses by aerobic organisms to prevent tissue damage. Polar marine waters are regarded as a strongly prooxidant ecosystem, due to the high dissolved oxygen level and intense UV radiation during summer, while the deep waters are considered refuges against oxidative stress. In

  4. Direct radical scavenging activity of benzbromarone provides beneficial antioxidant properties for hyperuricemia treatment.

    PubMed

    Kadowaki, Daisuke; Sakaguchi, Shoichi; Miyamoto, Yohei; Taguchi, Kazuaki; Muraya, Nanako; Narita, Yuki; Sato, Keizo; Chuang, Victor Tuan Giam; Maruyama, Toru; Otagiri, Masaki; Hirata, Sumio

    2015-01-01

    Uric acid exerts an important antioxidant effect against external oxidative stress under physiological conditions. However, uric acid itself can increase oxidative stress via reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation in adipocytes and vascular cells. Uric acid transporter 1 is involved in the generation of this oxidative stress. Furthermore, uric acid locally activates the renin-angiotensin system, thus producing angiotensin II and subsequently increasing intracellular oxidative stress. Benzbromarone has been reported to suppress uric acid reabsorption via uric acid transporter 1 inhibition in renal tubular cells. In this study we evaluated the in vitro antioxidant effect of benzbromarone from several perspectives. First, the direct radical-trapping capacity of benzbromarone was measured by chemiluminescence assay and electron paramagnetic resonance spectroscopy. Second, the intracellular antioxidant activity of benzbromarone in hyperuricemia was evaluated using endothelial cells. In light of these results, benzbromarone is hypothesized directly to scavenge the superoxide anion radical. In addition, benzbromarone inhibited reactive oxygen species production that was induced by angiotensin II or uric acid in endothelial cells. These findings suggest that benzbromarone possesses the ability directly to scavenge radicals and may act as an antioxidant against uric acid and angiotensin II-induced oxidative stresses in endothelial cells at therapeutically achievable levels in blood. PMID:25757933

  5. COMPARATIVE STUDY ON THE ANTIOXIDANT PROPERTIES OF FLAVONOIDS OF GARCINIA KOLA SEEDS

    Microsoft Academic Search

    Adaramoye OA; Farombi EO; Adeyemi EO; Emerole GO

    2005-01-01

    Objective: Oxidative damage has been suggested to be a contributory factor in the development and compli- cations of atherosclerosis, and of recent the beneficial effects of antioxidants against some pathologies have gained considerable interest. We evaluated the protective effects of flavonoids from Garcinia kola seeds on the oxidation of human low- density lipoprotein (LDL) and their ability to scavenge reactive

  6. Anti-oxidant, pro-oxidant properties of tannic acid and its binding to DNA

    Microsoft Academic Search

    Nelofer S Khan; Aamir Ahmad; S. M Hadi

    2000-01-01

    Tannic acid has numerous food and pharmacological applications. It is an additive in medicinal products, and is used as a flavouring agent and as an anti-oxidant in various foods and beverages. We have previously shown that tannic acid in the presence of Cu(II) causes DNA degradation through generation of reactive oxygen species. On the other hand, it exhibits antimutagenic and

  7. Wheat bran particle size influence on phytochemical extractability and antioxidant properties

    Technology Transfer Automated Retrieval System (TEKTRAN)

    It is unknown if particle size plays a role in extracting health promoting compounds in wheat bran because the extraction of antioxidant and phenolic compounds with particle size reduction has not been well documented. In this study, unmilled whole bran (coarse treatment) was compared to whole bran ...

  8. The in vitro and in vivo antioxidant properties of seabuckthorn ( Hippophae rhamnoides L.) seed oil

    Microsoft Academic Search

    Hung-Chih Ting; Yu-Wen Hsu; Chia-Fang Tsai; Fung-Jou Lu; Ming-Chih Chou; Wen-Kang Chen

    2011-01-01

    The antioxidant capacity of seabuckthorn (Hippophae rhamnoides L.) seed oil was investigated with a number of established in vitro assays and in an in vivo study of carbon tetrachloride (CCl4)-induced oxidative stress in mice. The results showed that DPPH radical scavenging activity, ferrous ion chelating activity, reducing power and inhibition of lipid peroxidation activity all increased with increasing concentrations of

  9. Effect of Biotic Elicitors on Enrichment of Antioxidant Properties and Induced Isoflavones in Soybean

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The antioxidant activities of seed extracts from three soybean varieties derived before/after germination and with/without the use of elicitors were compared. Isoflavone content was determined by C18 reverse phase high-performance chromatography coupled with a photodiode array detector. Antioxidan...

  10. Influence of ripening stages on antioxidant properties of papaya fruit (Carica papaya L.)

    NASA Astrophysics Data System (ADS)

    Addai, Zuhair Radhi; Abdullah, Aminah; Mutalib, Sahilah Abd.

    2013-11-01

    Papaya (Carica papaya L. cv Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to determine the phenolic compounds and antioxidants activity in different ripening stages of papaya fruit. The fruits were harvested at five different, stages RS1, RS2, RS3, RS4, and RS5 corresponding to 12, 14, 16, 18, and 20 weeks after anthesis, respectively. Papayas fruit at five different stage of ripening were obtained from farms at Pusat Flora Cheras, JabatanPertanian and Hulu Langat Semenyih, Selangor, Malaysia. The antioxidants activity were analyzed using the total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The analyses were conducted in triplicate and the data were subjected to statistical analysis using SPSS. The results showed significant differences (P< 0.05) were found at different stages of ripening. The total phenol content TPC, TFC, FRAP and DPPH values increased significantly (P<0.05) with the ripening process. The results showed the important role of the ripening stage in increasing the antioxidant content of papaya fruits.

  11. The Antioxidant Properties and Inhibitory Effects on HepG2 Cells of Chicory Cultivated Using Three Different Kinds of Fertilizers in the Absence and Presence of Pesticides.

    PubMed

    Yook, Jin-Seon; Kim, Mina; Pichiah, Pichiah BalasubramanianTirupathi; Jung, Su-Jin; Chae, Soo-Wan; Cha, Youn-Soo

    2015-01-01

    The objective of this study was to explore the antioxidant levels and anticancer properties of chicory cultivated using three different kinds of fertilizers (i.e., developed, organic, and chemical) in the presence and absence of pesticides. Phenolic phytochemicals, including total polyphenols and flavonoids, and antioxidant activities, including reducing power, ABTS+ and DPPH radical scavenging activity, were analyzed using several antioxidant assays. HepG2 cell viability was analyzed using the MTT assay. The antioxidant properties of chicory were found to increase when cultivated with chemical fertilizer in the absence of pesticides. On the other hand, antioxidant capacity was higher in chicory cultivated with eco-developed fertilizer even in the presence of pesticides. Chicory grown using eco-developed or organic fertilizer was more effective in suppressing the proliferation of HepG2 cells when compared to chicory grown with chemical fertilizer. This effect was time dependent, regardless of treatment with or without pesticides. In conclusion, the antioxidant activity of chicory were affected by the presence or absence of pesticides. However, developed and organic fertilizers showed a strong anti-proliferative effect against HepG2 cells, regardless of the presence or absence of pesticides. PMID:26140439

  12. Impact of processing on the phenolic profiles of small millets: evaluation of their antioxidant and enzyme inhibitory properties associated with hyperglycemia.

    PubMed

    Pradeep, P M; Sreerama, Yadahally N

    2015-02-15

    The effects of germination, steaming and microwave treatments of whole grain millets (barnyard, foxtail and proso) on their phenolic composition, antioxidant activities and inhibitory properties against ?-amylase and ?-glucosidase were investigated. Compositional analysis of phenolics by HPLC revealed that vanillic and ferulic acids were the principal phenolic acids and kaempferol was the predominant flavonoid found in raw millets. Different processing treatments brought about relevant changes in the composition and content of certain phenolic acids and flavonoids in processed millets. Phenolic extracts of raw and processed millets exhibited multiple antioxidant activities and are also potent inhibitors of ?-amylase and ?-glucosidase. In general, germinated millets showed highest phenolic content as well as superior antioxidant and enzyme inhibitory activities. These results suggest that germinated millet grains are potential source of phenolic antioxidants and also great sources of strong natural inhibitors for ?-amylase and ?-glucosidase. PMID:25236251

  13. Isolation and Structure Characterization of an Antioxidative Glycopeptide from Mycelial Culture Broth of a Medicinal Fungus

    PubMed Central

    Wu, Jian-Yong; Chen, Xia; Siu, Ka-Chai

    2014-01-01

    A novel glycopeptide (Cs-GP1) with an average molecular weight (Mw) of 6.0 kDa was isolated and purified by column chromatography from the lower Mw fraction of exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis Cs-HK1. Its carbohydrate moiety was mainly composed of glucose and mannose at 3.2:1.0 mole ratio, indicating an O-linked glycopeptide. The peptide chain contained relatively high mole ratios of aspartic acid, glutamic acid and glycine (3.3–3.5 relative to arginine) but relatively low ratios of tyrosine and histidine. The peptide chain sequence analyzed after trypsin digestion by LC-MS was KNGIFQFGEDCAAGSISHELGGFREFREFLKQAGLE. Cs-GP1 exhibited remarkable antioxidant capacity with a Trolox equivalent antioxidant capacity of 1183.8 ?mol/g and a ferric reducing ability of 611.1 ?mol Fe(II)/g, and significant protective effect against H2O2-induced PC12 cell injury at a minimum dose of 10 ?g/mL. This is the first report on the structure and bioactivity of an extracellular glycopeptide from the Cordyceps species. PMID:25268609

  14. Isolation and structure characterization of an antioxidative glycopeptide from mycelial culture broth of a medicinal fungus.

    PubMed

    Wu, Jian-Yong; Chen, Xia; Siu, Ka-Chai

    2014-01-01

    A novel glycopeptide (Cs-GP1) with an average molecular weight (Mw) of 6.0 kDa was isolated and purified by column chromatography from the lower Mw fraction of exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis Cs-HK1. Its carbohydrate moiety was mainly composed of glucose and mannose at 3.2:1.0 mole ratio, indicating an O-linked glycopeptide. The peptide chain contained relatively high mole ratios of aspartic acid, glutamic acid and glycine (3.3-3.5 relative to arginine) but relatively low ratios of tyrosine and histidine. The peptide chain sequence analyzed after trypsin digestion by LC-MS was KNGIFQFGEDCAAGSISHELGGFREFREFLKQAGLE. Cs-GP1 exhibited remarkable antioxidant capacity with a Trolox equivalent antioxidant capacity of 1183.8 ?mol/g and a ferric reducing ability of 611.1 ?mol Fe(II)/g, and significant protective effect against H2O2-induced PC12 cell injury at a minimum dose of 10 ?g/mL. This is the first report on the structure and bioactivity of an extracellular glycopeptide from the Cordyceps species. PMID:25268609

  15. Delivery systems for natural antioxidant compounds: Archaeosomes and archaeosomal hydrogels characterization and release study.

    PubMed

    González-Paredes, Ana; Clarés-Naveros, Beatriz; Ruiz-Martínez, María Adolfina; Durbán-Fornieles, Juan José; Ramos-Cormenzana, Alberto; Monteoliva-Sánchez, Mercedes

    2011-12-15

    The aim of this study was to use archaeosomes, a novel kind of liposomes made up by archaeal polar lipids, both multilamellars (MLVs) and unilamellars (SUVs), as a topical delivery system for natural antioxidant compounds recovered from olive mill waste. For comparative purpose an analogue formulation of phosphatidylcholine liposomes was prepared. SUVs were smaller than MLVs ones, showing size values smaller than 200nm, which was maintained during the stability study. Transmission electron microscopy showed spherical morphology for conventional liposomes while archaeosomes had more irregular membranes. Vesicle encapsulation efficiency was quite similar in both formulations and was enough to ensure a good antioxidant activity. Stability studies were performed one month after the preparation of formulations, which showed a high stability with no change in the initial characteristics of the suspensions. Furthermore, the possibility of incorporating the liposomal suspensions in different excipients (Carbopol-940(®) and Pluronic-127(®)) for topical administration was studied. In order to evaluate the release behaviour of the different systems prepared, in vitro diffusion studies were carried out using vertical diffusion Franz cells. In both cases the incorporation of the vesicles into the gels lead in a sustained release for 24h. Archaeosome gels released a similar amount of phenolic compounds regardless the excipient used, while in liposomal gels great release differences were found between carbopol and pluronic gel. PMID:21986112

  16. Structural characterization of a polysaccharide from Chrysanthemum morifolium flowers and its antioxidant activity.

    PubMed

    Zheng, Changping; Dong, Qun; Chen, Huanjun; Cong, Qifei; Ding, Kan

    2015-10-01

    The flowers of Chrysanthemum morifolium were extracted by boiling water, and a water-soluble polysaccharide (CMJA0S2) with a molecular weight of 6.5kDa was isolated by anion-exchange chromatography on a DEAE-cellulose column and gel permeation chromatography on a Sephacryl S-300 HR column. Monosaccharide composition analysis indicated that CMJA0S2 was composed of galactose, glucose, mannose and arabinose in molar ratio of 4.1: 3.3: 1.0: 2.3. According to linkage analysis, partial acid hydrolysis and NMR spectra, the backbone was shown to contain 1, 4-linked ?-Galp, 1, 4-linked ?-Glcp and 1, 4-linked ?-Manp, with branches substituted at C-6 of 1, 4-linked ?-Manp by 1, 6-linked ?-Galp and at O-6 of partial 1, 4-linked ?-Galp substituted by T-linked ?-Glcp. About 40% of 1, 6-linked ?-Galp with T-linked ?-Glcp was substituted at O-3 by ?-Araf-(1?[5)-?-Araf-(1]3. The anti-oxidative analysis showed that CMJA0S2 could scavenge the DPPH radicals and relieve the damage of PC12 cells caused by H2O2, thus it could be regarded as a potential natural antioxidant. PMID:26076607

  17. Theoretical insights on the antioxidant activity of edaravone free radical scavengers derivatives

    NASA Astrophysics Data System (ADS)

    Cerón-Carrasco, José P.; Roy, Hélène M.; Cerezo, Javier; Jacquemin, Denis; Laurent, Adèle D.

    2014-04-01

    The prediction of antioxidant properties is not straightforward due to the complexity of the in vivo systems. Here, we use theoretical descriptors, including the potential of ionization, the electrodonating power and the spin density distribution, to characterize the antioxidant capacity of edaravone (EDV) derivatives. Our computations reveal the relationship between these parameters and their potential bioactivity as free radical scavengers. We conclude that more efficient antioxidants could be synthesized by tuning the R1 and R2 positions of the EDV structure, rather than modifying the R3 group. Such modifications might improve the antioxidant activity in neutral and deprotonated forms.

  18. Biomolecular characterization of wild sicilian oregano: phytochemical screening of essential oils and extracts, and evaluation of their antioxidant activities.

    PubMed

    Tuttolomondo, Teresa; La Bella, Salvatore; Licata, Mario; Virga, Giuseppe; Leto, Claudio; Saija, Antonella; Trombetta, Domenico; Tomaino, Antonio; Speciale, Antonio; Napoli, Edoardo M; Siracusa, Laura; Pasquale, Andrea; Curcuruto, Giusy; Ruberto, Giuseppe

    2013-03-01

    An extensive survey of wild Sicilian oregano was made. A total of 57 samples were collected from various sites, followed by taxonomic characterization from an agronomic perspective. Based on morphological and production characteristics obtained from the 57 samples, cluster analysis was used to divide the samples into homogeneous groups, to identify the best biotypes. All samples were analyzed for their phytochemical content, applying a cascade-extraction protocol and hydrodistillation, to obtain the non volatile components and the essential oils, respectively. The extracts contained thirteen polyphenol derivatives, i.e., four flavanones, seven flavones, and two organic acids. Their qualitative and quantitative characterization was carried out by LC/MS analyses. The essential oils were characterized using a combination of GC-FID and GC/MS analyses; a total of 81 components were identified. The major components of the oils were thymol, p-cymene, and ?-terpinene. Cluster analysis was carried out on both phytochemical profiles and resulted in the division of the oregano samples into different chemical groups. The antioxidant activity of the essential oils and extracts was investigated by the Folin-Ciocalteau (FC) colorimetric assay, by UV radiation-induced peroxidation in liposomal membranes (UV-IP test), and by determining the O(2)(?-)-scavenging activity. PMID:23495158

  19. Effect of organic acid pretreatment on some physical, functional and antioxidant properties of flour obtained from three unripe banana cultivars.

    PubMed

    Anyasi, Tonna A; Jideani, Afam I O; Mchau, Godwin R A

    2015-04-01

    Unripe banana flour (UBF) obtained from organic acid pretreatment of pulp from three non-commercial cultivars were profiled for physical, functional and antioxidant properties. UBF showed marked significant differences (p<0.05) in colour (CIEL(?)a(?)b(?) and CIELCH) and water holding capacity with no significant difference in oil holding capacity. The total polyphenol content (TPC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) differed significantly with M-red UBF recording high TPC (1130.39 ± 27.26 mg GAE/100g d.w.) at 10 g/L citric acid pretreatment. Correlation analysis between TPC and DPPH showed very strong positive correlation for Mabonde UBF in citric and lactic acid pretreatment (r = 0.999, p < 0.01; r = 0.985, p < 0.01), while inverse correlation was recorded in M-red UBF for ascorbic and lactic acid pretreatment (r = -0.031; r = -0.137). Organic acid pretreatment enhances the physical and antioxidant properties of UBF hitherto absent in composite food formulations. PMID:25442586

  20. Antioxidant and functional properties of collagen hydrolysates from Spanish mackerel skin as influenced by average molecular weight.

    PubMed

    Chi, Chang-Feng; Cao, Zi-Hao; Wang, Bin; Hu, Fa-Yuan; Li, Zhong-Rui; Zhang, Bin

    2014-01-01

    In the current study, the relationships between functional properties and average molecular weight (AMW) of collagen hydrolysates from Spanish mackerel (Scomberomorous niphonius) skin were researched. Seven hydrolysate fractions (5.04 ? AMW ? 47.82 kDa) from collagen of Spanish mackerel skin were obtained through the processes of acid extraction, proteolysis, and fractionation using gel filtration chromatography. The physicochemical properties of the collagen hydrolysate fractions were studied by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), gel filtration chromatography, scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). The results indicated that there was an inverse relationship between the antioxidant activities and the logarithm of the AMW of the hydrolysate fractions in the tested AMW range. However, the reduction of AMW significantly enhanced the solubility of the hydrolysate fractions, and a similar AMW decrease of the hydrolysate fractions negatively affected the emulsifying and foaming capacities. This presented as a positive correlation between the logarithm of AMW and emulsion stability index, emulsifying activity index, foam stability, and foam capacity. Therefore, these collagen hydrolysates with excellent antioxidant activities or good functionalities as emulsifiers could be obtained by controlling the effect of the digestion process on the AMW of the resultant hydrolysates. PMID:25090114

  1. Evaluation of bioactive components and antioxidant and anticancer properties of citrus wastes generated during bioethanol production.

    PubMed

    Im, Soon Jae; Kim, Jae-Hoon; Kim, Min Young

    2014-04-01

    In the bioethanol production process employing citrus peels, a large amount of enzymatic hydrolyzed residues is generated as waste material. The bioactive compounds, and antioxidant and anticancer activities of these residues were investigated in the present study. Hydrolyzed citrus residues exhibited similar antioxidant activity as the unhydrolyzed control, which was positively correlated to the contents of total phenols, flavonoids and total carotenoid. Some flavonoids (naringin, naringenin, hesperetin and neohesperidin) and two high value co-products (D-limonene and galacturonic acid) were detected only in hydrolyzed residues. In addition, hydrolyzed residues showed antiproliferative activity and sub-G1 arrest in human melanoma A375 and colon cancer HCT116 cells. These results provide an alternative use for hydrolyzed citrus residues in the functional food, cosmetic and pharmaceutical industries. PMID:24868862

  2. Antioxidant and antimicrobial properties of water soluble polysaccharide from Arachis hypogaea seeds.

    PubMed

    Jiang, Shengjuan; Ma, Yuhan; Yan, Dazhuang

    2014-10-01

    The water soluble crude polysaccharide (AHP) was obtained from the aqueous extracts of the Arachis hypogaea seeds through hot water extraction followed by ethanol precipitation. Antioxidant activities and inhibitory activities against the bacteria of AHP were investigated. AHP at 2 mg/mL was found to inhibit the formation of superoxide anion (55.33 %) and hydroxyl radicals (30.85 %), to scavenge the DPPH radical (57.43 %) and to chelate iron ion (27.83 %) in in vitro systems. AHP also exhibited the antibacterial activities. AHP at 12.5 mg/mL could inhibit the growth of the Gram-positive bacteria, implying that the Gram-positive bacteria were more sensitive to AHP than the Gram-negative bacteria. Polysaccharide with antioxidant and antibacterial activities in the "Chang Sheng Guo" further increased the nutritive values of peanuts as well as the natural health product potential. PMID:25328235

  3. Studies on Mastication Stage Binding of Antioxidants

    Microsoft Academic Search

    Shanti A. Avirah; Rani Joseph

    1995-01-01

    Paraphenylenediamine and diphenylamine were chemically attached to natural rubber during mastication. The rubber bound antioxidants were characterized by TLC, 'H-NMR, IR and TGA. The efficiency and permenance of these bound antioxidants were compared with a conventional amine type antioxidant in filled natural rubber vulcanizates. The rubber bound antioxidants were found to be less volatile and less extractable as compared to

  4. Influence of enzymatic hydrolysis and enzyme type on the nutritional and antioxidant properties of pumpkin meal hydrolysates.

    PubMed

    Venuste, Muhamyankaka; Zhang, Xiaoming; Shoemaker, Charles F; Karangwa, Eric; Abbas, Shabbar; Kamdem, Patrick Eugene

    2013-04-30

    Nutritional and antioxidant properties of pumpkin meal and their hydrolysates prepared by hydrolysis with alcalase, flavourzyme, protamex or neutrase were evaluated. The hydrolysis process significantly increased protein content from 67.07% to 92.22%. All the essential amino acids met the Food and Agriculture Organization of United Nations/World Health Organization (WHO/FAO) suggested requirements for children and adults. The amino acid score (AAS) of meal was increased from 65.59 to 73.00 except for flavourzyme (62.97) and protamex (62.50). The Biological Value (BV) was increased from 53.18 to 83.44 except for protamex (40.97). However hydrolysis decreased the Essential Amino Acid/Total Amino Acid ratio (EAA/TAA) from 32.98% to 29.43%. Protein Efficiency Ratio (PER) was comparable to that of good quality protein (1.5) except for flavourzyme hydrolysate which had PER1 = 0.92, PER2 = 1.03, PER3 = 0.38. The in vitro protein digestibility (IVPD) increased from 71.32% to 77.96%. Antioxidant activity increased in a dose-dependent manner. At 10 mg mL(-1), the hydrolysates had increased 1,1-diphenyl-2-picrylhydrazy (DPPH) radical scavenging activities from 21.89% to 85.27%, the reducing power increased from Abs(700nm) 0.21 to 0.48. Metal (Iron) chelating ability was improved from 30.50% to 80.03% at 1 mg mL(-1). Hydrolysates also showed better capabilities to suppress or delay lipid peroxidation in a linoleic acid model system. Different proteases lead to different Degrees of Hydrolysis (DH), molecular weight (MW) distribution, amino acid composition and sequence, which influenced the nutritional properties and antioxidant activities of the hydrolysates. Alcalase was the most promising protease in production of pumpkin protein hydrolysates with improved nutritional quality, while flavourzyme was best in production of hydrolysates with improved antioxidative activity among various assays. These results showed that hydrolysates from by-products of pumpkin oil-processing might serve as alternative sources of dietary proteins with good nutritional quality, and protection against oxidative damage. PMID:23591974

  5. Antioxidant Properties of Extracts from Medicinal Plants Popularly Used in Taiwan

    Microsoft Academic Search

    Lie-Fen Shyur; Jieh-Hen Tsung; Je-Hsin Chen; Chih-Yang Chiu; Chiu-Ping Lo

    We have examined antioxidant activities of twenty-six medicinal herbal extracts that have been popularly used as folk medicines in Taiwan. The results of scavenging DPPH radical activity show that, among the 26 tested medicinal plants, Ludwigia octovalvis, Vitis thunbergii, Rubus parvifolius, Lindernia anagallis, and Zanthoxylum nitidum exhibited strong activities and their IC50 values for DPPH radicals were 4.6, 24, 27,

  6. Antioxidant properties of whey protein hydrolysates as measured by three methods

    Microsoft Academic Search

    Adriena Dryáková; Anne Pihlanto; Pertti Marnila; Ladislav ?urda; Hannu J. T. Korhonen

    2010-01-01

    Four microbial proteases (Alcalase, Flavourzyme, Neutrase and Protamex) were used for the preparation of whey protein hydrolysates.\\u000a The aim of this research was to find out whether these hydrolysates can be used as a source of whey derived antioxidants.\\u000a Hydrolyzed samples, including their unhydrolyzed protein solutions were tested by the ABTS (2,2?-azinobis-(3-ethylbenzothiazoline-6-sulfonic\\u000a acid) decolorization assay, by the total radical-trapping potential

  7. Antioxidant properties of cold-pressed black caraway, carrot, cranberry, and hemp seed oils

    Microsoft Academic Search

    Liangli Lucy Yu; Kequan Kevin Zhou; John Parry

    2005-01-01

    Cold-pressed black caraway, carrot, cranberry, and hemp seed oils were extracted with methanol and evaluated for radical-scavenging activities against ABTS+ and DPPH, chelating activity, oxygen radical absorbing capacity (ORAC), and total phenolic contents (TPC). All the oil extracts had significant antioxidant activities. The ORAC value ranged from 28 to 220 ?mol TE\\/g oil for the cold-pressed hemp, carrot, and black

  8. Antioxidant Property of an Ethanol Extract of the Stem of Opuntia ficus-indica var. Saboten

    Microsoft Academic Search

    Jeong-Chae Lee; Hak-Ryul Kim; Ju Kim; Yong-Suk Jang

    2002-01-01

    An ethanol extract of the stem of Opuntia ficus-indica var. saboten (OFS) was assessed to determine the mechanism(s) of its antioxidant activity. The ethanol extract exhibited a concentration-dependent inhibition of linoleic acid oxidation in a thiocyanate assay system. In addition, the OFS extract showed dose-dependent free-radical scavenging activity, including DPPH radicals, superoxide anions (O2¥-), and hydroxyl radicals (¥OH), using different

  9. Theoretical investigation of the conformational, electronic and antioxidant properties of azaleatin, isorhamnetin and quercetagetin

    Microsoft Academic Search

    K. Senthil kumar; R. Kumaresan

    2012-01-01

    Gas phase and solvent phase reaction enthalpies related to the individual steps of three phenolic antioxidants in the view of action mechanisms – hydrogen atom transfer (HAT), single electron transfer–proton transfer (SET–PT) and sequential proton loss electron transfer (SPLET) – have been calculated using DFT\\/B3LYP\\/6-311++G** method. The analysis of the theoretical bond dissociation enthalpy (BDE) values for all OH sites

  10. Antibacterial and antioxidant properties of Ramulus Cinnamomi using supercritical CO 2 extraction

    Microsoft Academic Search

    Ming-Tsai Liang; Cheng-Hong Yang; Shun-Tai Li; Cheng-San Yang; Hsueh-Wei Chang; Ching-Shen Liu; Thau-Ming Cham; Li-Yeh Chuang

    2008-01-01

    In this study, crude extracts of Ramulus Cinnamomi from supercritical carbon dioxide under various extraction conditions were examined for their antioxidant and antibacterial\\u000a activity. The extractions were conducted in the range of 4,000–6,000 psi and 40–50 °C, and the solvent to feed ratio of the\\u000a extraction was 30. The antibacterial activity was tested on the clinical drug-resistant strains, including 27 Acinetobacter baumannii,

  11. Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves.

    PubMed

    Sharma, Sonia; Vig, Adarsh Pal

    2013-01-01

    In the present study, methanol and aqueous extracts of Parkinsonia aculeata L. leaves were prepared and analyzed for phytochemical analysis and antioxidant potential in different in vitro assays. Antioxidant activity was studied using DPPH, CUPRAC, reducing power assay, deoxyribose degradation (site and nonsite specific), ferric reducing antioxidant potential (FRAP), ferric thiocyanate (FTC), thiobarbituric acid (TBA), and molybdate ion reduction, respectively. The total phenolic contents of the methanol and aqueous leaf extract were 39?mg?GAE/g and 38?mg?GAE/g, whereas flavonoid contents of these extracts were found to be 0.013?mg?RE/g and 0.006?mg?RE/g, respectively. From the two extracts, the methanol extract shows maximum inhibition (%) of 57.82%, 71.23%, 48.26%, 69.85%, and 52.78% in DPPH, nonsite- and site-specific, FTC, and TBA assays and absorbance of 0.669 and 0.241 in reducing power and CUPRAC assays at the highest concentration tested. UPLC analysis was done to determine the presence of various types of polyphenols present in plant extracts. PMID:24348173

  12. Caryocar brasiliense supercritical CO2 extract possesses antimicrobial and antioxidant properties useful for personal care products

    PubMed Central

    2014-01-01

    Background The cosmetic and pharmaceutical industries have an increasing interest in replacing synthetic antimicrobials in dermatological products due to increased microbial resistance to conventional antimicrobial agents. Pequi (Caryocar brasiliense) is a native fruit tree of the Brazilian Cerrado, specifically used in cosmetics, in the food industry, and for medicinal purposes. Leishmanicidal and antifungal activities have been reported previously. This study was designed to evaluate the antimicrobial and antioxidant activities of a C. brasiliense extract obtained by supercritical CO2 extraction. Methods The minimum inhibitory concentrations (MICs) against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were determined by the classical microdilution method. Antiseptic activity against these organisms was evaluated by the plate diffusion method. The antioxidant potential of the extract was evaluated using a method based on the oxidation of 2,2?-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The extract’s chemical profile was analyzed for the presence of alkaloids, saponins, anthraquinones, steroids, tannins, flavonoids, and phenolic compounds according to standard colorimetric methods. Results The C. brasiliense supercritical CO2 extract exhibits antimicrobial activity against all bacteria tested. It also possesses antioxidant activity, when compared to a vitamin E standard. Conclusions The C. brasiliense supercritical CO2 extract may be useful for the development of personal care products, primarily for antiseptic skin products that inactivate, reduce, prevent, or arrest the growth of microorganisms with the inherent intent to mitigate or prevent disease as well as products that minimize damage caused by free radicals. PMID:24565304

  13. Enrichment and antioxidant properties of flavone C-glycosides from trollflowers using macroporous resin.

    PubMed

    Sun, Yan; Yuan, Hongyan; Hao, Lili; Min, Chunyan; Cai, Jinna; Liu, Jiangyun; Cai, Peilie; Yang, Shilin

    2013-11-01

    Orientin, vitexin and other flavone C-glycosides are functional ingredients abundant in trollflowers. In this study, an efficient method for enrichment of these ingredients from the flowers of Trollius chinensis Bunge was developed using macroporous resin. Separation characteristics of six typical macroporous resins were investigated by static adsorption/desorption and dynamic separation experiments, and HPD450 was selected as optimal one. Dynamic adsorption/desorption experiments on HPD450 columns were conducted to obtain the optimal parameters, followed by a scale-up experiment. Six fractions, TC-1-TC-3 together with their acid hydrolysates were further prepared to evaluate their antioxidant capacities by 2,2-diphenyl-1-picrylhydrazyl radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonate) radical cation scavenging assays. They all have notable concentration-dependent antioxidant activities, with TC-1 showing strong antioxidant capacity higher than orientin. The separation process was high-efficient, low-cost and environmental-friendly, which could afford a potential approach for industrial applications. PMID:23768390

  14. Antioxidant properties and evaluation of phytochemical composition of Salvia verbenaca L. extracts at different developmental stages.

    PubMed

    Ben Farhat, Mouna; Chaouch-Hamada, Rym; Sotomayor, Jose A; Landoulsi, Ahmed; Jordán, María J

    2015-03-01

    Changes in the contents and composition of polyphenolics and resulting antioxidant activities of S. verbenaca by-products were investigated at three phenological stages (flowering, early fruiting and late fruiting stages). The highest accumulation of total phenolics was detected at the flowering stage (58.36 mg GAE/g DW). HPLC analysis of methanolic extracts showed the prevalence of methyl carnosate (821.45-919.82 ?g/g DW) and rosmarinic acid (544.51-649.26 ?g/g DW). Phenolic diterpenes (1056.90-1148.42 ?g/g DW) was the most represented class of compounds. Three complementary tests namely, DPPH(•) (IC50 value, 49.22 ?g/mL) and ABTS(•+)-radical scavenging assays (146.86 ?M TE/mg) and FRAP reducing power test (188.93 mM Fe(II)/mg) were used to evaluate the antioxidant capacity and showed the best performance at the early fruiting period. The current study evidenced the significant effect of phenophase on antioxidants and contributed to valorize S. verbenaca extracts as a source of functional phenolic compounds. PMID:25645661

  15. In vitro and in vivo antioxidant properties of extracts from Coptis chinensis inflorescence.

    PubMed

    Ban, Xiaoquan; Huang, Bo; He, Jingsheng; Chen, Yuxin; Zeng, Hong; Han, Li; Wang, Youwei

    2011-06-01

    The inflorescence of cultivated Coptis chinensis has been valued for tea production for many years in China. The antioxidant activities of C. chinensis inflorescence extracts prepared by various solvents were investigated by using several established in vitro systems: 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS), ?,?-diphenyl-?-picrylhydrazyl (DPPH) and superoxide radical scavenging assays, reducing power assay, and ferrothiocyanate (FTC) and thiobarbituric acid (TBA) assays. The results showed that the 70% ethanol extract (EE) had the strongest antioxidant activity in vitro among the various extracts. Based on the in vitro results, EE was used to evaluate the antioxidant activity of C. chinensis inflorescence in vivo. The liver and kidney of intoxicated animals showed a significant decrease in superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels, while the malondialdehyde (MDA) level showed a significant increase. These changes were significantly reversed after treatment with EE and the standard vitamin E. Thus, the C. chinensis inflorescence may be a valuable natural source that can be applicable to food industries. PMID:21523412

  16. Antiproliferative and Antioxidant Properties of Anthocyanin Rich Extracts from Blueberry and Blackcurrant Juice

    PubMed Central

    Diaconeasa, Zori?a; Leopold, Loredana; Rugin?, Dumitri?a; Ayvaz, Huseyin; Socaciu, Carmen

    2015-01-01

    The present study was aimed at evaluating the antiproliferative potential of anthocyanin-rich fractions (ARFs) obtained from two commercially available juices (blueberry and blackcurrant juices) on three tumor cell lines; B16F10 (murine melanoma), A2780 (ovarian cancer) and HeLa (cervical cancer). Individual anthocyanin determination, identification and quantification were done using HPLC-MS. Antioxidant activity of the juices was determined through different mechanism methods such as DPPH and ORAC. For biological testing, the juices were purified through C18 cartridges in order to obtain fractions rich in anthocyanins. The major anthocyanins identified were glycosylated cyanidin derivatives. The antiproliferative activity of the fractions was tested using the MTT assay. The antiproliferative potential of ARF was found to be associated with those bioactive molecules, anthocyanins due to their antioxidant potential. The results obtained indicated that both blueberry and blackcurrants are rich sources of antioxidants including anthocyanins and therefore these fruits are highly recommended for daily consumption to prevent numerous degenerative diseases. PMID:25622252

  17. Antiproliferative and antioxidant properties of anthocyanin rich extracts from blueberry and blackcurrant juice.

    PubMed

    Diaconeasa, Zori?a; Leopold, Loredana; Rugin?, Dumitri?a; Ayvaz, Huseyin; Socaciu, Carmen

    2015-01-01

    The present study was aimed at evaluating the antiproliferative potential of anthocyanin-rich fractions (ARFs) obtained from two commercially available juices (blueberry and blackcurrant juices) on three tumor cell lines; B16F10 (murine melanoma), A2780 (ovarian cancer) and HeLa (cervical cancer). Individual anthocyanin determination, identification and quantification were done using HPLC-MS. Antioxidant activity of the juices was determined through different mechanism methods such as DPPH and ORAC. For biological testing, the juices were purified through C18 cartridges in order to obtain fractions rich in anthocyanins. The major anthocyanins identified were glycosylated cyanidin derivatives. The antiproliferative activity of the fractions was tested using the MTT assay. The antiproliferative potential of ARF was found to be associated with those bioactive molecules, anthocyanins due to their antioxidant potential. The results obtained indicated that both blueberry and blackcurrants are rich sources of antioxidants including anthocyanins and therefore these fruits are highly recommended for daily consumption to prevent numerous degenerative diseases. PMID:25622252

  18. Antioxidative properties of Murraya koenigii leaf extracts in accelerated oxidation and deep-frying studies.

    PubMed

    Nor, Fatihanim Mohd; Suhaila, Mohamed; Aini, Idris Nor; Razali, Ismail

    2009-01-01

    Murraya koenigii leaf extract antioxidant potentials were evaluated in palm olein using accelerated oxidation storage and deep-frying studies at 180 degrees C for up to 40 h. The extracts (0.2%) retarded oil oxidation and deterioration significantly (P<0.05), slightly less effectively than 0.02% butylated hydroxytoluene in tests such as the peroxide value, anisidine value, iodine value, free fatty acid, Oxidative Stability Index, and polar and polymer compound content. Sensory evaluation on French fries indicated that the extract was useful in improving colour, flavour and overall acceptability and the quality of the fried product. All samples were more acceptable by panellists, especially after the 40th hour frying, compared with those similarly fried in the control oils and the oil containing butylated hydroxytoluene. M. koenigii leaf extract, had a polyphenol content of 109.5+/-0.3 mg gallic acid equivalents/g extract, and contain a heat-stable antioxidant that could be a natural alternative to synthetic antioxidants for the industry. PMID:19488917

  19. Preparation of organogel with tea polyphenols complex for enhancing the antioxidation properties of edible oil.

    PubMed

    Shi, Rong; Zhang, Qiuyue; Vriesekoop, Frank; Yuan, Qipeng; Liang, Hao

    2014-08-20

    Food-grade organogels are semisolid systems with immobilized liquid edible oil in a three-dimensional network of self-assembled gelators, and they are supposed to have a broad range of potential applications in food industries. In this work, an edible organogel with tea polyphenols was developed, which possesses a highly effective antioxidative function. To enhance the dispersibility of the tea polyphenols in the oil phase, a solid lipid-surfactant-tea polyphenols complex (organogel complex) was first prepared according to a novel method. Then, a food-grade organogel was prepared by mixing this organogel complex with fresh peanut oil. Compared with adding free tea polyphenols, the organogel complex could be more homogeneously distributed in the prepared organogel system, especially under heating condition. Furthermore, the organogel loading of tea polyphenols performed a 2.5-fold higher antioxidation compared with other chemically synthesized antioxidants (butylated hydroxytoluene and propyl gallate) by evaluating the peroxide value of the fresh peanut oil based organogel in accelerated oxidation conditions. PMID:25089366

  20. Investigation of antioxidant properties of metal ascorbates and their mixtures by voltammetry

    NASA Astrophysics Data System (ADS)

    Vtorushina, A. N.; Nikonova, E. D.

    2015-04-01

    The paper describes modern ways for selection of anti-radical substances. Molding of such components with a carbon-based material decreases the rate of its oxidative destruction. Addition of such a component to a carbon-based material decreases the rate of its oxidative destruction. The purpose of this study is to determine the antioxidant activity of ascorbates metals (Ca, Mg, Li, Co, Fe), used in the practice of medicine, as well as mixtures based on them together with well-known antioxidants. In this article we examine the effect of metals on the process of ascorbate oxygen electroreduction. From these ascorbates lithium and magnesium ascorbate showed the greatest activity toward cathode oxygen reduction process. Also mixtures with well-known examined antioxidants ascorbate (glucose, dihydroquercetin) were investigated at different concentrations of components. It is shown that the multicomponent mixtures exhibit lower activity than the individual drugs. Recommended the creation of drugs on the basis of ascorbate Mg and Li with not more than 3 number of components.

  1. Biofunctional Constituents from Liriodendron tulipifera with Antioxidants and Anti-Melanogenic Properties.

    PubMed

    Li, Wei-Jen; Lin, Yi-Chieh; Wu, Pei-Fang; Wen, Zhi-Hong; Liu, Po-Len; Chen, Chung-Yi; Wang, Hui-Min

    2013-01-01

    From the stems of Liriodendron tulipifera, seventeen known compounds have been extracted, isolated and purified. By using spectroscopic analysis, the structures of these pure constituents were determined as three lignans, four steroids and ten benzenoids. Identified compounds were screened for antioxidant abilities using: 1,1-diphenyl-2-picrylhydrazul (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging free radical activity assays; metal chelating power test; and ferric reducing/antioxidant power (FRAP) examination. The result revealed that seventeen compounds had potential anti-oxidative capabilities. In addition, the anti-tyrosinase effect was determined by calculating the hydroxylation of L-tyrosine to L-dopa and the oxidization of L-dopa to dopaquinone, according to in vitro mushroom tyrosinase evaluation platform. Furthermore, based on assays on B16F10 cell line, our data suggest that five compounds isolated from L. tulipifera would be able to inhibit tyrosinase activity and reduce the melanin content in animal cells. Therefore, some of the examined compounds could be potentially used in the cosmetic skin whitening business, therapeutic applications or the food industry. PMID:23322020

  2. In vitro antioxidant properties of a ginseng intestinal metabolite IH-901

    PubMed Central

    Lee, Nam-Jin; Lee, Jung Won; Sung, Jong Hwan

    2011-01-01

    IH-901 (20-O-?-D-glucopyranosyl-20(S)-protopanaxadiol or compound K) is a final intestinal bacterial metabolite of ginseng in humans. It has various pharmacologic effects such as antiaging, immunopotentiation, antistress, and antimetastatic activities. We analyzed the antioxidant activities of IH-901 using several assays including: total antioxidant activity, reductive potential, 1,1-diphenyl-2-picryl-hydrazyl, hydroxyl, superoxide and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, a nitric oxide scavenging assay and a lipid peroxidation assay. At concentrations of 25 and 100 µg/mL, IH-901 inhibited lipid peroxidation of a linoleic acid emulsion with a potency comparable to ascorbic acid and butylated hydroxyanisole. The reductive potential of IH-901 increased in a concentration-dependent manner. IH-901 exhibited strong DPPH, hydroxyl, superoxide and ABTS radical scavenging activities. IH-901 was also an effective inhibitor of lipid peroxidation, although IH-901 had only a mild scavenging activity against nitric oxide. These results suggest that IH-901 may be a useful antioxidant agent against reactive oxygen species. PMID:21998612

  3. Characterization of increased phenolic compounds from fermented Bokbunja (Rubus coreanus Miq.) and related antioxidant activity.

    PubMed

    Ju, Hyun Kyoung; Cho, Eun Jung; Jang, Moon Hee; Lee, Yong Yook; Hong, Soon Sun; Park, Jeong Hill; Kwon, Sung Won

    2009-04-01

    This study examined the changes in the phenolic acid-content and antioxidant activity of Rubi Fructus (RF), the fruit of Rubus coreanus Miq., after fermentation with yeast (Saccharomyces cerevisiae). The phenolic acids were fractionated into three forms, free (Fr. A), ester (Fr. B), and insoluble-bound phenolic acids (Fr. C) and quantified by high performance liquid chromatography with a diode array detector (HPLC-DAD). This method was validated and allowed the successful identification of 11 phenolic acids in the RF extracts. HPLC-DAD analysis of the samples showed substantial increases in the levels of protocatechuic, vanillic and p-coumaric acid as the result of yeast fermentation. The total phenolic content (TPH) was also increased by fermentation. The total phenolics in Fr. A and Fr. B increased from 117 to 173 mg GAE/100 g and from 488 to 578 mg GAE/100 g, respectively. The total phenolics in Fr. C decreased from 264 to 175 mg GAE/100 g. The antioxidant activity of the fermented RF was measured as the 1,1-diphenoly-2-picrylhydrazyl (DPPH) radical scavenging capacity, which is expressed as the IC(50). The IC(50) for Fr. A and Fr. B decreased from 5.9 to 4.0 mg/ml (mg of dried RF equiv./ml) and from 1.2 to 0.8 mg/ml, respectively. In Fr. C, the IC(50) value increased from 2.1 to 2.8 mg/ml. In summary, the fermented RF had a higher total phenolic content and better DPPH radical-scavenging activity than the unfermented material. PMID:19179032

  4. Antioxidant genes of the emerald ash borer (Agrilus planipennis): gene characterization and expression profiles.

    PubMed

    Rajarapu, Swapna Priya; Mamidala, Praveen; Herms, Daniel A; Bonello, Pierluigi; Mittapalli, Omprakash

    2011-06-01

    Phytophagous insects frequently encounter reactive oxygen species (ROS) from exogenous and endogenous sources. To overcome the effect of ROS, insects have evolved a suite of antioxidant defense genes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione peroxidase (GPX). The emerald ash borer (Agrilus planipennis Fairmaire), an exotic invasive insect pest from Asia has killed millions of ash trees and continues to invade North America at a rapid pace. From an on-going expressed sequence tag (EST) project of A. planipennis larval tissues, we identified ESTs coding for a Cu-Zn SOD (ApSOD1), a CAT (ApCAT1) and a GPX (ApGPX1). A multiple sequence alignment of the derived A. planipennis sequences revealed high homology with other insect sequences at the amino acid level. Phylogenetic analysis of ApSOD1 grouped it with Cu-Zn SODs of other insect taxa. Quantitative real time PCR (qRT-PCR) analysis in different larval tissues (midgut, fat body, Malpighian tubule and cuticle) revealed high mRNA levels of ApCAT1 in the midgut. Interestingly, high mRNA levels for both ApSOD1 and ApGPX1 were observed in the Malpighian tubules. Assay of mRNA levels in developmental stages (larva, prepupa and adults) by qRT-PCR indicated high transcript levels of ApCAT1 and ApGPX1 in larval and prepupal stages with a decline in adults. On the other hand, the transcript levels of ApSOD1 were observed to be constitutive in all the developmental stages assayed. Results obtained reflect a plausible role of these A. planipennis antioxidant genes in quenching ROS from both diet (ash allelochemicals) as well as endogenous sources. These studies further help in understanding the adaptation/invasiveness of A. planipennis. PMID:21439289

  5. Antioxidant properties of fruiting bodies, mycelia, and fermented products of the culinary-medicinal king oyster mushroom, Pleurotus eryngii (higher Basidiomycetes), with high ergothioneine content.

    PubMed

    Liang, Chih-Hung; Ho, Kung-Jui; Huang, Ling-Yi; Tsai, Ching-Hsuan; Lin, Shin-Yi; Mau, Jeng-Leun

    2013-01-01

    The culinary-medicinal king oyster mushroom Pleurotus eryngii is known to contain ergothioneine, and its products, including fruiting bodies, mycelia, and solid-state fermented products (adlay and buckwheat), were prepared to study their antioxidant properties. Fruiting bodies, regular and Hi-Ergo mycelia, and fermented products contained 2.05, 1.68, 5.76, 0.79-0.80 mg/g of ergothioneine, respectively. On the basis of the results obtained, P. eryngii products had effective antioxidant activity, reducing power, and scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals and chelating ability on ferrous ions. Hi-Ergo mycelia was the most effective in the first 3 antioxidant properties in addition to its ergothioneine content. In addition, fruiting bodies were more effective in all antioxidant properties than regular mycelia. For ethanolic and hot water extracts from mycelia and fruiting bodies, the correlation coefficients between total phenol contents and each antioxidant attribute were 0.483-0.921. Overall, P. eryngii products with high amounts of ergothioneine could be used beneficially as a functional food. PMID:23662614

  6. Ripening stage and extraction method effects on physical properties, polyphenol composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds.

    PubMed

    Rebey, Iness Bettaieb; Kefi, Sarra; Bourgou, Soumaya; Ouerghemmi, Ines; Ksouri, Riadh; Tounsi, Moufida Saidani; Marzouk, Brahim

    2014-12-01

    The effects of two extraction methods, used at three ripening stages on the total polyphenol contents and the antioxidant activities of Cumin (Cuminum cyminum L.) seed extracts were studied. The ripening stage effect on some physical properties of cumin seed was significant. The increase of dry matter (from 10.3 to 87.5%) during ripeness was correlated negatively with that of moisture content (from 89.7 to 12.5%). Besides results showed that the full ripe seeds were richer on polyphenols and condensed tannin than unripe ones, and consequently exhibited higher antioxidant activities. However, the unripe seeds had a higher total flavonoid content compared to those of half ripe and full ripe ones. The comparison of two extraction methods showed that soxhlet extracts contained the greatest amount of polyphenols and flavonoids, while maceration samples exhibited higher antiradical and bleaching power assay. Total phenolic contents and IC50 (concentration required to cause a 50% DPPH inhibition) values in cumin seed during their maturation allowed to conclude that antioxidant activity does not depend only on the high content of total phenolics but also on the phenolic composition. A total of 19 phenolic compounds were successfully identified by HPLC analysis during the ripening of cumin seeds. Rosmarinic acid was the major phenolic acid for the unripe seeds. Furthermore, half ripe and full ripe seeds were dominated by p-coumaric acid. These compounds might be considered as interesting bioactive natural substances that may be used in several fields, such as nutraceuticals, cosmetics and agro-food industry. PMID:25277369

  7. Comparison of some antioxidant properties of plant extracts from Origanum vulgare, Salvia officinalis, Eleutherococcus senticosus and Stevia rebaudiana.

    PubMed

    Vaško, Ladislav; Vašková, Janka; Fejer?áková, Andrea; Mojžišová, Gabriela; Porá?ová, Janka

    2014-08-01

    Phenolic compounds from plants are known for their antioxidant properties and have been proposed as therapeutic agents to counteract oxidative stress. However, under normal circumstances, the body only receives a very small amount of these substances in the diet. We have investigated the effect of extracts from known and frequently used plants as part of diet, food seasoning, medicinal tea, and sweetener at different concentrations on the ability to scavenge free radicals, to affect antioxidant enzymes, and finally in the survival of cancer cell lines. We found extract concentrations of about 100 ?g.ml(-1) more indicative in the assessment of all parameters investigated. Ginseng possessed a very good ability to scavenge superoxide and hydroxyl radicals, while stevia also manifested significant effects against hydroxyl radicals. Both extracts also showed NO decomposition ability. The antioxidant defense system against the excessive production of radicals in mitochondria was sufficient. In contrast, the range of operating concentrations for sage and oregano mainly presented no significant effects against reactive oxygen and nitrogen species. Taken together with the significantly reduced activity of glutathione peroxidase, this led to the depletion of glutathione. The demonstrated modulation of redox state capability was sufficient to affect the viability of all tested cancer cell lines, but especially A-549, CEM and HeLa by oregano extract. Results support the promising role of the tested extracts as a source of compounds for further in vivo studies with the ability to powerfully interfere with or modify the redox state of cells according to the type of disease, which is expected to be associated with oxidative stress. PMID:24737278

  8. In vitro evaluation of glutathione peroxidase (GPx)-like activity and antioxidant properties of an organoselenium compound.

    PubMed

    Ibrahim, Mohammad; Muhammad, Niaz; Naeem, Muhammad; Deobald, Anna Maria; Kamdem, Jean Paul; Rocha, Joao Batista Teixeira

    2015-08-01

    The amine based diselenide, (Z)-N-(4-methylbenzylidene)-1-(2-((2-(1-((E)-4-methyl benzylideneamino)ethyl)phenyl)diselanyl)phenyl)ethanamine ethyl)phenyl) diselanyl) phenyl) ethylimino) methyl)phenol (Compound A) an organoselenium compound that can mimic endogenous antioxidant enzymes, such as glutathione peroxidase (GPx), and diphenyl diselenide (PhSe)2 were tested against lipid peroxidation induced by sodium nitroprusside (SNP) and Fe(II) in rat brain, interaction with 1,1-diphenyl-2-picrylhydrazyl stable free radical (DPPH) and glutathione peroxidase (GPx) like antioxidant activities with H2O2 or tBuOOH as substrates and with PhSH as thiol co-substrates as well as their ability to oxidize thiols were evaluated. From this study, we concluded that Compound A catalyze the reduction of H2O2 with thiol was ?2-fold more active than (PhSe)2) in both tBuOOH and H2O2 systems when PhSH was used as a substrate. (PhSe)2 exhibited an increased ability to oxidize thiols while Compound A was not a good substrate for the oxidation of thiol used namely DTT and Cystine and showed DPPH radical-scavenging activity, while (PhSe)2 did not present radical scavenging activity. Compound A (amine based diselenide) presented better antioxidant profiles than (PhSe)2 against lipid peroxidation. The results clear showed that nitrogen atom in the Compound A can have a profound effect on their pharmacological properties. PMID:25862122

  9. Antioxidant activity of alkyl gallates and glycosyl alkyl gallates in fish oil in water emulsions: Relevance of their surface active properties and of the type of emulsifier.

    PubMed

    González, María J; Medina, Isabel; Maldonado, Olivia S; Lucas, Ricardo; Morales, Juan C

    2015-09-15

    The antioxidant activity of gallic acid and a series of alkyl gallates (C4-C18) and glycosylated alkyl gallates (C4-C18) on fish oil-in-water emulsions was studied. Three types of emulsifiers, lecithin, Tween-20 and sodium dodecyl sulphate (SDS) were tested. A nonlinear behavior of the antioxidant activity of alkyl gallates when increasing alkyl chain length was observed for emulsions prepared with lecithin. Medium-size alkyl gallates (C6-C12) were the best antioxidants. In contrast, for emulsions prepared with Tween-20, the antioxidants seem to follow the polar paradox. Glucosyl alkyl gallates were shown previously to be better surfactants than alkyl gallates. Nevertheless, they exhibited a worse antioxidant capacity than their corresponding alkyl gallates, in emulsions prepared with lecithin or Tween-20, indicating the greater relevance of having three OH groups at the polar head in comparison with having improved surfactant properties but just a di-ortho phenolic structure in the antioxidant. PMID:25863628

  10. Characterization, antioxidant and cytotoxicity evaluation of green synthesized silver nanoparticles using Cleistanthus collinus extract as surface modifier

    SciTech Connect

    Kanipandian, Nagarajan [Laboratory of Aquabiotics/Nanoscience, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu (India); Kannan, Soundarapandian [Proteomics and Molecular Cell Physiology Laboratory, Department of Zoology, Periyar University, Salem 636 011, Tamil Nadu (India); Ramesh, Ramar; Subramanian, Periyasamy [Laboratory of Aquabiotics/Nanoscience, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu (India); Thirumurugan, Ramasamy, E-mail: ramthiru72@gmail.com [Laboratory of Aquabiotics/Nanoscience, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu (India)

    2014-01-01

    Graphical abstract: The figure is the TEM image of green synthesized silver nanoparticles from Cleistanthus collinus. In this investigation we have used the poisonous plant as a reducing and capping agent. This is a first time data to synthesis the metal nanoparticles using poisonous plant. - Highlights: • A hitherto unreported venomous plant mediated AgNPs synthesis. • The particle size is observed in the range of 20–40 nm. • Surface morphology of the well-dispersed silver nanoparticles is studied using SEM and TEM. • Crystalline nature of AgNPs is confirmed by X-ray diffraction analysis. • Antioxidant activities of green synthesized AgNPs are tested in vitro. - Abstract: We report, here a simple green method for the preparation of silver nanoparticles (AgNPs) using the plant extract of Cleistanthus collinus as potential phyto reducer. The synthesized AgNPs were characterized by UV–vis spectra, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The obtained results confirmed that the AgNPs were crystalline in nature and the morphological studies reveal the spherical shape of AgNPs with size ranging from 20 to 40 nm. The in vitro antioxidant activity of AgNPs showed a significant effect on scavenging of free radicals. The cytotoxicity study exhibited a dose-dependent effect against human lung cancer cells (A549) and normal cells (HBL-100), the inhibitory concentration (IC{sub 50}) were found to be 30 ?g/mL and 60 ?g/mL respectively. The in vivo histopathology of mouse organs proved that AgNPs does not possess toxic effect and can be extensively applied in biomedical sciences.

  11. Evaluation of antioxidant, rheological, physical and sensorial properties of wheat flour dough and cake containing turmeric powder.

    PubMed

    Park, S H; Lim, H S; Hwang, S Y

    2012-10-01

    The effects of addition of turmeric powder (0%, 2%, 4%, 6% and 8%) were examined in order to obtain an antioxidant-enriched cake with good physico-chemical and sensorial properties. The rheological properties of doughs were evaluated using dynamic rheological measurements. Physical properties, curcumin content, radical scavenging activity (RSA-DPPH assay) and sensory analysis (hedonic test) of the supplemented cake were determined. Addition of turmeric powder up to 8% caused significant changes on dough characteristics and on cake rheological properties. The highest curcumin (203?mg/kg) and RSA-DPPH activity (45%) were achieved in the cake having the highest percentage of turmeric powder (8%); however, this sample showed the worst results regarding the rheological properties. Moreover, by sensory evaluation this cake sample was not acceptable. A 6% substitution of wheat flour with turmeric powder showed acceptable sensory scores which were comparable to those of 0-4% turmeric cakes. This indicated that up to 6% level of turmeric powder might be included in cake formulation. PMID:23144239

  12. Antioxidant content of foods

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plant-based foods, especially fruits, vegetables, and nuts, contain bioactive components which have various biological functions, including free radical scavenging and metal chelating (antioxidant), inhibition of lipid peroxidation, anti-inflammatory properties, etc. Oxidative stress may contribute...

  13. Angiotensin-converting enzyme inhibitory activity and antioxidant properties of Nepeta crassifolia Boiss & Buhse and Nepeta binaludensis Jamzad.

    PubMed

    Tundis, Rosa; Nadjafi, Farsad; Menichini, Francesco

    2013-04-01

    This article reports phytochemical and biological studies on Nepeta binaludensis and Nepeta crassifolia. Both species were investigated for their angiotensin-converting enzyme (ACE) inhibitory activity and antioxidant properties through three in vitro models [2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assay]. Aerial parts were extracted with methanol and partitioned between water and subsequently n-hexane, ethyl acetate and n-butanol. N. binaludensis methanol extract exerted significantly higher reducing power (1.9 ?M Fe(II)/g) than did the positive control butylhydroxytoluene (63.2??M Fe(II)/g) in FRAP assay. The highest DPPH radical scavenging activity was found for N. crassifolia, with IC50 values of 9.6 and 12.1 µg/mL for ethyl acetate and n-butanol fractions, respectively. n-Butanol fraction of both species showed the highest ACE inhibitory activity, with IC50 values of 59.3 and 81.7 µg/mL for N. binaludensis and N. crassifolia, respectively. Phytochemical investigations resulted in the isolation of ursolic acid, oleanolic acid, apigenin, luteolin and ixoroside. Apigenin-7-O-glucoside, 8-hydroxycirsimaritin and cirsimaritin were furthermore identified in N. crassifolia ethyl acetate-soluble fraction. Nepetanudoside B was isolated from the n-butanol fraction of N. binaludensis. PMID:22693035

  14. The Anticancer, Antioxidant and Antimicrobial Properties of the Sesquiterpene ?-Caryophyllene from the Essential Oil of Aquilaria crassna.

    PubMed

    Dahham, Saad S; Tabana, Yasser M; Iqbal, Muhammad A; Ahamed, Mohamed B K; Ezzat, Mohammed O; Majid, Aman S A; Majid, Amin M S A

    2015-01-01

    The present study reports a bioassay-guided isolation of ?-caryophyllene from the essential oil of Aquilaria crassna. The structure of ?-caryophyllene was confirmed using FT-IR, NMR and MS. The antimicrobial effect of ?-caryophyllene was examined using human pathogenic bacterial and fungal strains. Its anti-oxidant properties were evaluated by DPPH and FRAP scavenging assays. The cytotoxicity of ?-caryophyllene was tested against seven human cancer cell lines. The corresponding selectivity index was determined by testing its cytotoxicity on normal cells. The effects of ?-caryophyllene were studied on a series of in vitro antitumor-promoting assays using colon cancer cells. Results showed that ?-caryophyllene demonstrated selective antibacterial activity against S. aureus (MIC 3 ± 1.0 µM) and more pronounced anti-fungal activity than kanamycin. ?-Caryophyllene also displayed strong antioxidant effects. Additionally, ?-caryophyllene exhibited selective anti-proliferative effects against colorectal cancer cells (IC50 19 µM). The results also showed that ?-caryophyllene induces apoptosis via nuclear condensation and fragmentation pathways including disruption of mitochondrial membrane potential. Further, ?-caryophyllene demonstrated potent inhibition against clonogenicity, migration, invasion and spheroid formation in colon cancer cells. These results prompt us to state that ?-caryophyllene is the active principle responsible for the selective anticancer and antimicrobial activities of A. crassnia. ?-Caryophyllene has great potential to be further developed as a promising chemotherapeutic agent against colorectal malignancies. PMID:26132906

  15. Bioprotective properties of Dragon's blood resin: In vitro evaluation of antioxidant activity and antimicrobial activity

    PubMed Central

    2011-01-01

    Background Food preservation is basically done to preserve the natural characteristics and appearance of the food and to increase the shelf life of food. Food preservatives in use are natural, chemical and artificial. Keeping in mind the adverse effects of synthetic food preservatives, there is a need to identify natural food preservatives. The aims of this study were to evaluate in vitro antioxidant and antimicrobial activities of Dragon's blood resin obtained from Dracaena cinnabari Balf f., with a view to develop safer food preservatives. Methods In this study, three solvents of varying polarity were used to extract and separate the medium and high polarity compounds from the non-polar compounds of the Dragon's blood resin. The extracts were evaluated for their antimicrobial activity against the food borne pathogens. The antioxidant activities of the extracts were assessed using DPPH and ABTS radical scavenging, FRAP, metal chelating and reducing power assays. Total phenolics, flavonoids and flavonols of extracts were also estimated using the standard methods. Results Phytochemical analysis of extracts revealed high phenolic content in CH2Cl2 extract of resin. Free radical scavenging of CH2Cl2 extract was found to be highest which is in good correlation with its total phenolic content. All test microorganisms were also inhibited by CH2Cl2 extract. Conclusions Our result provide evidence that CH2Cl2 extract is a potential source of natural antioxidant compounds and exhibited good inhibitory activity against various food borne pathogens. Thus, CH2Cl2 extract of Dragon's blood resin could be considered as possible source of food preservative. PMID:21329518

  16. An uncommon redox behavior sheds light on the cellular antioxidant properties of ergothioneine.

    PubMed

    Servillo, Luigi; Castaldo, Domenico; Casale, Rosario; D'Onofrio, Nunzia; Giovane, Alfonso; Cautela, Domenico; Balestrieri, Maria Luisa

    2015-02-01

    Ergothioneine (ESH), an aromatic thiol occurring in the human diet and which accumulates in particular cells, is believed to act as an antioxidant. However, its redox mechanism remains unclear and it does not seem to provide any advantage compared to other antioxidants, such as alkylthiols, which are better reducing agents and generally present in cells at higher levels. Here, we investigated by ESI-MS the products of ESH oxidation produced by neutrophils during oxidative burst and, to further elucidate ESH redox behavior, we also analyzed the oxidation products of the reaction of ESH with hypochlorite in cell-free solutions. Indeed, neutrophils are the main source of hypochlorite in humans. Furthermore, we also tested other biologically relevant oxidants, such as peroxynitrite and hydrogen peroxide. Our results indicate that treatment of human neutrophils with phorbol 12-myristate 13-acetate in the presence of ESH leads to a remarkable production of the sulfonated form (ESO3H), a compound never described before, and hercynine (EH), the desulfurated form of ESH. Similar results were obtained when ESH was subjected to cell-free oxidation in the presence of hypochlorite, as well as hydrogen peroxide or peroxynitrite. Furthermore, when the disulfide of ESH was reacted with those oxidants, we found that it was also oxidized, with production of EH and ESO3H, whose amount was dependent on the oxidant strength. These data reveal a unique ESH redox behavior, entirely different from that of alkylthiols, and suggest a mechanism, so far overlooked, through which ESH performs its antioxidant action in cells. PMID:25483556

  17. Characterization of the Phytochemical Constituents of Taif Rose and Its Antioxidant and Anticancer Activities

    PubMed Central

    Abdel-Hameed, El-Sayed S.; Bazaid, Salih A.; Salman, Mahmood S.

    2013-01-01

    Ward Taifi (Taif rose) is considered one of the most important economic products of Taif, Saudi Arabia. In this study both fresh and dry Taif rose were biologically and phytochemically investigated. The 80% methanol extracts and n-butanol fractions of dry and fresh Taif rose had high radical scavenging activity toward artificial 1,1-diphenyl picrylhydrazyl (DPPH)• radical with SC50 values range 5.86?12.24?µg/ml whereas the aqueous fractions showed weak activity. All samples had in vitro anticancer activity toward HepG2 with IC50 < 20?µg/ml which fall within the criteria of the American Cancer Institute. High positive correlation appeared between the antioxidant activity and total phenolics whereas there is no correlation between total phenolics and anticancer activity. The LC-ESI(? ve)-MS analysis of all extracts indicate the presence of phenolic compounds belonging to hydrolysable tannins and flavonol glycosides. In conclusion, the presence of this is considered to be the first phytochemical report that identifies the major compounds in dry and fresh roses using HPLC-ESI-MS. The methanol extracts and its n-butanol and aqueous fractions for both fresh and dry Taif rose could be used as preventive and therapeutic effective natural agents for diseases in which free radicals involved after more in vitro and in vivo studies. PMID:24282813

  18. Comparison of the phenolic-dependent antioxidant properties of coconut oil extracted under cold and hot conditions

    Microsoft Academic Search

    Kapila N. Seneviratne; Chamil D. HapuarachchI; Sagarika Ekanayake

    2009-01-01

    The antioxidant activities of coconut oil extracted under hot and cold conditions were compared. The coconut oil extracted under hot conditions (HECO) contained more phenolic substances than the coconut oil extracted under cold conditions (CECO). The antioxidant potential of HECO was higher than that of CECO as demonstrated by DPPH assay, deoxyribose assay and in vivo assay of serum antioxidant

  19. Microstructural Characterization of Material Properties and Damage in Asphalt Composites 

    E-print Network

    Mohammad Khorasani, Sara

    2013-05-03

    used for the characterization of fatigue cracking. This test method applies cyclic loads on asphalt composites, damaging them, and in the process determines the viscoelastic properties of the composite throughout the test. The nano...

  20. THE USE OF CHEMICAL MARKERS AND MODEL STUDIES TO ASSESS THE IN VITRO PRO AND ANTIOXIDATIVE PROPERTIES OF METHYLXANTHINE-RICH BEVERAGES

    Microsoft Academic Search

    Richard H. Stadler

    2001-01-01

    Chemical markers and model studies are useful in predicting the stability and shelf life of foods and beverages in food technology and chemistry. Chemical modification induced by food processing has an impact on the reactivity and chemical properties of the food constituents, frequently giving rise to new molecules with enhanced antioxidative activity and potential health benefit. In the case of

  1. Artocarpus gomezianus aided green synthesis of ZnO nanoparticles: luminescence, photocatalytic and antioxidant properties.

    PubMed

    Suresh, D; Shobharani, R M; Nethravathi, P C; Pavan Kumar, M A; Nagabhushana, H; Sharma, S C

    2015-04-15

    We report green synthesis of multifunctional ZnO nanoparticles (NPs) using Artocarpus gomezianus (AG) extract as fuel by solution combustion synthesis. The formation of NPs was confirmed by powder XRD, SEM, TEM and UV-Visible studies. The NPs were subjected for photoluminescence, photodegradative and antioxidant studies. XRD data reveals that the ZnO NPs possess wurtzite structure. UV-Visible spectrum shows absorbance maximum at 370 nm which corresponds to the energy band gap of 3.3 eV. Morphology studies indicate the highly porous nature of the NPs. PL spectra of NPs found to display very interesting blue, green and red emissions upon excitation at 325 nm. The NPs exhibit potential photocatalytic activity towards the degradation of methylene blue (MB) dye upon exposure to sun light and UV light. ZnO NPs found to have considerable antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals. The study successfully demonstrates a simple and eco-friendly method for the synthesis of efficient multifunctional ZnO nanoparticles using green synthetic approach. PMID:25668693

  2. Feruloylacetone as the model compound of half-curcumin: synthesis and antioxidant properties.

    PubMed

    Feng, Jian-Ying; Liu, Zai-Qun

    2011-04-01

    In order to clarify the contribution of phenolic and enolic hydroxyl group to the antioxidant capacity of feruloylacetone, a model compound of half-curcumin, 6-(p-hydroxy-m-methoxyphenyl)-5-hexene-2,4-dione (FT), 6-(p-benzyloxy-m-methoxyphenyl)-5-hexene-2,4-dione (BMFT), 6-(m,p-dihydroxyphenyl)-5-hexene-2,4-dione (DDFT), 6-(p-hydroxy-m-methoxyphenyl)hexane-2,4-dione (DHFT), 6-(p-hydroxy-m-methoxyphenyl)-5-hexene-2,4-diol (THFT), and ethyl 2-(p-hydroxy-m-methoxybenzylidene)-3-oxobutanoate (EOFT) were synthesized. The radical-scavenging abilities of these compounds were tested by trapping 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) cationic radical (ABTS(+·)), 2,2'-diphenyl-1-picrylhydrazyl (DPPH), and galvinoxyl radicals. The reductive capacities were screened by quenching singlet oxygen and by inhibiting the oxidation of linoleic acid. They were also employed to inhibit the oxidation of DNA mediated by hydroxyl radical and 2,2'-azobis(2-amidinopropane hydrochloride) (AAPH). In addition, they were applied to protect erythrocytes against AAPH- and hemin-induced hemolysis. The obtained results revealed that the antioxidant capacity of half-curcumin was derived from the phenolic-OH and the conjugated linkage between phenolic and enolic-OH. The enolic-OH itself cannot trap radicals. PMID:21330013

  3. Artocarpus gomezianus aided green synthesis of ZnO nanoparticles: Luminescence, photocatalytic and antioxidant properties

    NASA Astrophysics Data System (ADS)

    Suresh, D.; Shobharani, R. M.; Nethravathi, P. C.; Pavan Kumar, M. A.; Nagabhushana, H.; Sharma, S. C.

    2015-04-01

    We report green synthesis of multifunctional ZnO nanoparticles (NPs) using Artocarpus gomezianus (AG) extract as fuel by solution combustion synthesis. The formation of NPs was confirmed by powder XRD, SEM, TEM and UV-Visible studies. The NPs were subjected for photoluminescence, photodegradative and antioxidant studies. XRD data reveals that the ZnO NPs possess wurtzite structure. UV-Visible spectrum shows absorbance maximum at 370 nm which corresponds to the energy band gap of 3.3 eV. Morphology studies indicate the highly porous nature of the NPs. PL spectra of NPs found to display very interesting blue, green and red emissions upon excitation at 325 nm. The NPs exhibit potential photocatalytic activity towards the degradation of methylene blue (MB) dye upon exposure to sun light and UV light. ZnO NPs found to have considerable antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals. The study successfully demonstrates a simple and eco-friendly method for the synthesis of efficient multifunctional ZnO nanoparticles using green synthetic approach.

  4. Production of metabolites with antioxidant and emulsifying properties by antarctic strain Sporobolomyces salmonicolor AL?.

    PubMed

    Dimitrova, Stela; Pavlova, Kostantsa; Lukanov, Ludmil; Korotkova, Elena; Petrova, Ekaterina; Zagorchev, Plamen; Kuncheva, Margarita

    2013-01-01

    The Sporobolomyces salmonicolor AL(1) Antarctic strain was cultivated and two bioproducts were obtained: exopolysaccharide and biomass. The biologically active substances ergosterol, torularhodin, torulene, ?-carotene and CoQ(10) were extracted from the biomass and were quantified as follows: ergosterol 5.2?±?0.2 mg/g, torularhodin 458.3?±?24.5 ?g/g, torulene 273.7?±?14.5 ?g/g, ?-carotene 129.2?±?7.3 ?g/g and coenzyme Q(10) (CoQ(10)) 236.1?±?12.1 ?g/g. Their antioxidant activity was estimated according to the cathode voltammetry method. The most pronounced antioxidant activity (according to trolox) was exhibited by ?-carotene 3.78, followed by CoQ(10) 3.60, both of them being the main contributors to the total extract activity of 3.19. The biologically active metabolites in combination with exoglucomannan as emulsifier were used for the creation of model emulsion systems characterised by great stability. The absorption of UVA rays by the model emulsions was studied. PMID:23179285

  5. Potential nutraceutical food beverage with antioxidant properties from banana plant bio-waste (pseudostem and rhizome).

    PubMed

    Saravanan, Kandasamy; Aradhya, Somaradhya Mallikarjuna

    2011-10-01

    Banana plant biomass waste, viz. pseudostem (BPS) and rhizome (BR), contribute 30.81 and 12.67 per cent respectively. A negligible percentage of these were used for fresh consumption, otherwise they are waste and incinerated. In order to utilize these bio-wastes in a bioactive perspective, nutritional and nutraceutical components were studied from the juices and its Ready-To-Serve (RTS) beverage. When compared to BPS juice, BR juice showed higher total phenolic content (TPC) and total flavonoid content (TFC) of 341.44 mgGAE and 87.60 mgCE/200 ?L, respectively, and concomitantly exhibited high antioxidant activity (AOA) in all the assays tested, viz. DPPH radical scavenging activity (82.93%), superoxide radical scavenging activity (49.45%), metal chelation activity (48.32%) total reducing power (0.846 OD at 700 nm) and total antioxidant capacity (928 mmol ascorbic acid equivalents). High quantity of polyphenols present in BR juice resulted high AOA. Strong positive correlations were observed between TPC and TFC of BPS and BR juice with AOA assays. Among the different concentrations of RTS beverages prepared, 25% BPS juice and 20% BR juice with 15°brix TSS and 0.3% acidity were adjudged as best by sensory panelists. Thus, BPS and BR juice can be effectively used to produce new generation functional beverages. PMID:21915417

  6. Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties.

    PubMed

    Formagio, A S N; Ramos, D D; Vieira, M C; Ramalho, S R; Silva, M M; Zárate, N A H; Foglio, M A; Carvalho, J E

    2015-01-01

    The aim of this study was to evaluate the phenolic and flavonoids contents and the antioxidant and antitumoral activity of leaf and calyx methanolic extracts from Hibiscus sabdariffa (roselle) cultivated with poultry litter and organosuper® under three modes of application. The total phenolic content in the each extract was determined using the Folin-Ciocalteu reagent and for aluminium chloride flavonoids. The antioxidant parameters were analyzed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH.) free radical scavenging assay. An antitumor colorimetric assay using sulforhodamine B. The highest contents of phenolic and flavonoids were observed in leaf extracts (389.98 and 104.52 mg g-1, respectively) and calyx extracts (474.09 and 148.35 mg g-1, respectively) from plants cultivated with organosuper®, although these values did not differ significantly from those observed for the other treatments. The average IC50 of leaves (43.48 ?g mL-1) and calyces (37.15 ?g mL-1) demonstrated that both have substances that may contribute to free radical scavenging action. The methanol extract from calyces showed significant selective activity against a leukemia line (K-562), with IC50 values of 0.12 mg mL-1 (organosuper®) and 1.16 mg mL-1 (poultry litter), with concentration-dependent, cytotoxic and cytocidal effects. PMID:25945622

  7. Antioxidant and antiangiogenic properties of phenolic extract from Pleurotus tuber-regium.

    PubMed

    Lin, Shaoling; Lai, Tsz ching; Chen, Lei; Kwok, Hin fai; Lau, Clara Bik-san; Cheung, Peter C K

    2014-10-01

    Pleurotus tuber-regium (Fries) Singer (PTR), both an edible and a medicinal mushroom also known as tiger milk mushroom, has experienced growing popularity and economic importance due to its flavor, nutritive value, and medicinal effects. In this study, the antioxidant and antiangiogenic activities of a 60% ethanol extract (EE) obtained from the sclerotium of PTR were investigated. Typical phenolic compounds including protocatechuic, chlorogenic, syringic, ferulic, and folic acid were identified and quantified in EE by the HPLC-UV-ESI/MS analyses. EE possessed strong antioxidant activity and could dose-dependently inhibit vascular endothelial growth factor (VEGF)-induced human umbilical vein endothelial cells (HUVEC) migration and tube formation. qPCR results showed that VEGF-induced FGF, ANG-Tie, and MMP gene expression as well as VEGFR were down-regulated at the mRNA level after treated with EE, suggesting that multiple molecular targets related to angiogenesis was involved. Furthermore, EE also inhibited the formation of subintestinal vessel plexus (SIVs) in zebrafish embryos in vivo. All of these suggested that EE of PTR could be the source of potential inhibitors to target angiogenesis. PMID:25185869

  8. Antidiabetic and Antioxidant Properties of Triticum aestivum in Streptozotocin-Induced Diabetic Rats

    PubMed Central

    Mohan, Yogesha; Jesuthankaraj, Grace Nirmala; Ramasamy Thangavelu, Narendhirakannan

    2013-01-01

    The antidiabetic and antioxidant potential of Triticum aestivum were evaluated by using in vivo methods in normal and streptozotocin-induced diabetic rats. Diabetes was induced in the Wistar strain albino rats by injecting streptozotocin at a dose of 55?mg/kg body weight. Ethanolic extracts of Triticum aestivum at doses of 100?mg/kg body weight were administered orally for 30 days. Various parameters were studied and the treatment group with the extract showed a significant increase in the liver glycogen and a significant decrease in fasting blood glucose, glycosylated hemoglobin levels, and serum marker enzyme levels. The total cholesterol and serum triglycerides levels, low density lipoprotein, and very low density lipoprotein were also significantly reduced and the high density lipoprotein level was significantly increased upon treatment with the Triticum aestivum ethanol extract. A significant decrease in the levels of lipid peroxides, superoxide dismutase, and glutathione peroxidise and increase in the levels of vitamin E, catalase, and reduced glutathione were observed in Triticum aestivum treated diabetic rats. Thus, from this study we conclude that ethanolic extract of Triticum aestivum exhibited significant antihyperglycemic, hypolipidemic, and antioxidant activities in streptozotocin-induced diabetic rats. PMID:24416041

  9. Antioxidant and antigenotoxic properties of compounds isolated from Marrubium deserti de Noé.

    PubMed

    Zaabat, Nabila; Hay, Anne-Emmanuelle; Michalet, Serge; Darbour, Nicole; Bayet, Christine; Skandrani, Inès; Chekir-Ghedira, Leila; Akkal, Salah; Dijoux-Franca, Marie-Geneviève

    2011-12-01

    In our continual course toward the valorization of traditionally used endemic flora through the analysis of its chemobiodiversity, the phytochemical analysis of aerial parts of Marrubium deserti de Noé was undertaken. Dichloromethane and methanol extracts led to the isolation of terpenoid derivatives among which two were new labdane diterpenes named marrulibacetal A and desertine, respectively. Six of them were known compounds (a mixture of the isomers cyllenin A and 15-epi-cyllenin A, marrubiin, marrulactone, marrulibacetal and ?-stigmasterol) and seven known phenolic compounds were also isolated: apigenin and several 7-O-substituted derivatives (apigenin-7-O-?-neohesperidoside, apigenin-7-O-glucoside, terniflorin and apigenin-7-O-glucuronide) together with two phenylethanoid glucosides (acteoside and forsythoside B). The structures and relative configurations of the new compounds were elucidated by MS and a series of 1D and 2D NMR analyses. Some pure compounds have been evaluated for their antioxidant activities through different methods: DPPH and ABTS assays as well as CUPRAC assay. Genotoxic and antigenotoxic activities of extracts and pure compounds were also evaluated in vitro on Escherichia coli PQ37 cells by the SOS Chromotest. Some of the isolated compounds like phenylethanoid derivatives showed stronger antioxidant capacity than trolox and were also able to significantly inhibit ?-galactosidase induction caused by the mutagen agent nitrofurantoin. PMID:21924316

  10. Characterization of Thermal Properties of Depleted Uranium Metal Microspheres

    E-print Network

    Humrickhouse, Carissa Joy

    2012-07-16

    CHARACTERIZATION OF THERMAL PROPERTIES OF DEPLETED URANIUM METAL MICROSPHERES A Thesis by CARISSA JOY HUMRICKHOUSE Submitted to the O ce of Graduate Studies of Texas A&M University in partial ful llment of the requirements for the degree... of MASTER OF SCIENCE May 2012 Major Subject: Nuclear Engineering CHARACTERIZATION OF THERMAL PROPERTIES OF DEPLETED URANIUM METAL MICROSPHERES A Thesis by CARISSA JOY HUMRICKHOUSE Submitted to the O ce of Graduate Studies of Texas A&M University...

  11. Effect of copper-induced oxidative stress on sclerotial differentiation and antioxidant properties of Penicillium thomii PT95 strain.

    PubMed

    Zhang, Ze-Qing; Zhao, Wen-Jing; Long, Dan-Dan; Niu, Lin-Ru; Han, Jian-Rong

    2014-05-01

    Penicillium thomii PT95 strain was able to form abundant orange, sand-shaped sclerotia in which carotenoids were accumulated. The aim of this work was to determine the effects of copper-induced oxidative stress on the sclerotial differentiation and antioxidant properties of PT95 strain. The results showed that the time of exudates initiation, sclerotial initiation and sclerotial maturation of PT95 strain were advanced in 1-2 days under the copper-induced oxidative stress growth conditions. The analytical results of sclerotial biomass, carotenoids content in sclerotia showed that copper-induced oxidative stress favored the sclerotial differentiation and biosynthesis of carotenoids. Under the copper-induced oxidative stress growth conditions, the total phenolics content and DPPH free radical scavenging activity of sclerotia of this fungus were decreased as compared with the control. However, the oxidative stress induced by a lower amount of CuSO? in media could enhance significantly the reducing power of sclerotia. PMID:24307499

  12. Characterization of thiol-specific antioxidant 1 (TSA1) of Candida albicans.

    PubMed

    Shin, Duck Hyang; Jung, Saem; Park, So Jeong; Kim, Yeo Jung; Ahn, Jung Min; Kim, Wankee; Choi, Wonja

    2005-08-01

    We previously identified several proteins that are differentially expressed in pathogenic hyphae by comparing protein profiles of yeast and hyphae of Candida albicans. One of these, thiol-specific antioxidant 1 (TSA1), attracted our attention because it may play some roles in surviving an unfavourable oxidative environment created by host cells. Two alleles of the C. albicans TSA1 (CaTSA1) gene are located in opposite orientation on the same chromosome. Using PCR-directed disruption cassettes and URA-Blaster, a series of deletion mutants that lack one to four copies were constructed to examine the functions of CaTSA1. Northern and Western analyses showed that both the transcript and protein products of CaTSA1 decreased proportionally to the disrupted copy number and were completely absent in the null mutant, indicating that all four TSA1 copies are equally functional at the transcriptional level. Intracellular H2O2 increased by an order of magnitude in deletion mutants lacking three to four copies, suggesting that CaTsa1p is not a redundant H2O2 scavenger. CaTsa1p was indispensable for yeast-to-hyphal transition when C. albicans was cultured under oxidative stress. The level of its oxidized form increased approximately five-fold in hyphal cells, whereas that of the reduced form increased two-fold compared to yeast cells. The ratio of oxidized to reduced form was increased three-fold in hyphal cells. This overall increase was found to be controlled at the post-transcriptional level. Interestingly, CaTsa1p is translocated to the nucleus of hyphal cells. These findings may be of biological significance for differentiation and pathogenicity. PMID:16134099

  13. Characterization of the antioxidant enzyme, thioredoxin peroxidase, from the carcinogenic human liver fluke, Opisthorchis viverrini

    PubMed Central

    Suttiprapa, Sutas; Loukas, Alex; Laha, Thewarach; Wongkham, Sopit; Kaewkes, Sasithorn; Gaze, Soraya; Brindley, Paul J.; Sripa, Banchob

    2013-01-01

    The human liver fluke, Opisthorchis viverrini, induces inflammation of the hepatobiliary system. Despite being constantly exposed to inimical oxygen radicals released from inflammatory cells, the parasite survives for many years. The mechanisms by which it avoids oxidative damage are unknown. In this study, thioredoxin peroxidase (TPx), a member of the peroxiredoxin superfamily, was cloned from an O. viverrini cDNA library. O. viverrini TPx cDNA encoded a polypeptide of 212 amino acid residues, of molecular mass 23.57 kDa. The putative amino acid sequence shared 60-70% identity with TPXs from other helminths and from mammals, and phylogenetic analysis revealed a close relationship between TPxs from O. viverrini and other trematodes. Recombinant O. viverrini TPx was expressed as soluble protein in Escherichia coli. The recombinant protein dimerized, and its antioxidant activity was deduced by observing protection of nicking of supercoiled plasmid DNA by hydroxyl radicals. Antiserum raised against O. viverrini TPx recognized native proteins from egg, metacercaria and adult developmental stages of the liver fluke and excretory-secretory products released by adult O. viverrini. Immunolocalization studies revealed ubiquitous expression of TPx in O. viverrini organs and tissues. TPx was also detected in bile fluid and bile duct epithelial cells surrounding the flukes two weeks after infection of hamsters with O. viverrini. In addition, TPx was observed in the secondary (small) bile ducts where flukes cannot reach due to their large size. These results suggested that O. viverrini TPx plays a significant role in protecting the parasite against damage induced by reactive oxygen species from inflammation. PMID:18538872

  14. Evaluation of in vitro antioxidant properties of some traditional Sardinian medicinal plants: Investigation of the high antioxidant capacity of Rubus ulmifolius

    Microsoft Academic Search

    Stefano Dall’Acqua; Rinaldo Cervellati; Maria Cecilia Loi; Gabbriella Innocenti

    2008-01-01

    The antioxidant capacities of 11 botanical species used in the tradition of Sardinia as teas beverages or as decoction for medicinal purposes were evaluated using different in vitro methods (BR, TEAC, DPPH and FC). Among the various species, Rubus ulmifolius, resulted the more active with all the used methods. Phytochemical investigation on the extract yields in the isolation of several

  15. Materials thermal and thermoradiative properties/characterization technology

    NASA Technical Reports Server (NTRS)

    Dewitt, D. P.; Ho, C. Y.

    1989-01-01

    Reliable properties data on well characterized materials are necessary for design of experiments and interpretation of experimental results. The activities of CINDAS to provide data bases and predict properties are discussed. An understanding of emissivity behavior is important in order to select appropriate methods for non-contact temperature determination. Related technical issues are identified and recommendations are offered.

  16. Experimental characterization of the imaging properties of multifocal intraocular lenses

    Microsoft Academic Search

    Pier Giorgio Gobbi; Francesco Fasce; Stefano Bozza; Rosario Brancato

    2003-01-01

    Many different types of intraocular lenses (IOL) are currently available for implantation, both as crystalline lens replacements and as phakic refractive elements. Their optical design is increasingly sophisticated, including aspherical surface profiles and multi-zone multifocal structures, however a quantitative and comparative characterization of their imaging properties is lacking. Also a qualitative visualization of their properties would be very useful for

  17. Statistical Characterization of Damage Propagation Properties in Structural Health Monitoring

    Microsoft Academic Search

    Alexandra Coppe; Raphael T. Haftka; Nam-Ho Kim

    Structural health monitoring may provide readings that follow fatigue induced damage growth in service. This information may in turn be used to improve the characterization of the material properties that govern damage propagation for the monitored structure. These properties are often widely distributed between nominally identical structures because of differences in manufacturing and aging. The improved accuracy in damage growth

  18. Isolation and characterization of a novel glycosyltransferase that converts phloretin to phlorizin, a potent antioxidant in apple.

    PubMed

    Jugdé, Hélène; Nguy, Danny; Moller, Isabel; Cooney, Janine M; Atkinson, Ross G

    2008-08-01

    The dihydrochalcone phlorizin (phloretin 2'-glucoside) contributes to the flavor, color and health benefits of apple fruit and processed products. A genomics approach was used to identify the gene MdPGT1 in apple (Malus x domestica) with homology to the UDP-glycosyltransferase 88 family of uridine diphosphate glycosyltransferases that show specificity towards flavonoid substrates. Expressed sequence tags for MdPGT1 were found in all tissues known to produce phlorizin including leaf, flower and fruit. However, the highest expression was measured by quantitative PCR in apple root tissue. The recombinant MdPGT1 enzyme expressed in Escherichia coli glycosylated phloretin in the presence of [(3)H]-UDP-glucose, but not other apple antioxidants, including quercetin, naringenin and cyanidin. The product of phloretin and UDP-glucose co-migrated with an authentic phlorizin standard. LC/MS indicated that MdPGT1 could glycosylate phloretin in the presence of three sugar donors: UDP-glucose, UDP-xylose and UDP-galactose. This is the first report of functional characterization of a UDP-glycosyltransferase that utilizes a dihydrochalcone as its primary substrate. PMID:18573104

  19. Comparative Chemical Composition and Antioxidant Properties of the Essential Oils of three Sideritis libanotica Subspecies.

    PubMed

    Formisano, Carmen; Oliviero, Filomena; Rigano, Daniela; Arnold, Nelly Apostolides; Senatore, Felice

    2015-06-01

    The phytochemical composition of the essential oils of three Sideritis libanotica subspecies, namely S. libanotica ssp. libanotica, S. libanotica ssp. linearis and S. libanotica ssp. michroclamys, all collected in Lebanon, was analyzed by GC and GC-MS. The diterpene sideridiol was recognized as the main constituent of both S. libanotica ssp. libanotica (50.8%) and S. libanotica ssp. michroclamys (18.4%) oils, while hexadecanoic acid (10.5%) prevailed in S. libanotica ssp. linearis. The antioxidant activity of the oils was studied in two cell free systems by DPPH radical scavenging and ferric ion reduction (FRAP) assays; only S. libanotica ssp. linearis showed a moderate activity when assayed by the FRAP test (0.6 ± 0.01 mmol TE/mL). PMID:26197555

  20. New silibinin glyco-conjugates: synthesis and evaluation of antioxidant properties.

    PubMed

    Zarrelli, Armando; Romanucci, Valeria; Tuccillo, Concetta; Federico, Alessandro; Loguercio, Carmela; Gravante, Raffaele; Di Fabio, Giovanni

    2014-11-15

    New silibinin glyco-conjugates have been synthesized by efficient method and in short time. Exploiting our solution phase strategy, several structurally diverse silibinin glyco-conjugates (gluco, manno, galacto, and lacto-) were successfully realized in very good yields and in short time. In preliminary study to evaluate their antioxidant and neuroprotective activities new derivatives were subjected to DPPH free radical scavenging assay and the Xanthine oxidase (XO) inhibition models assay. Irrespective of the sugar moiety examined, new glyco-conjugates are more than 50 times water-soluble of silibinin. In the other hand they exhibit a radical scavenging activities slightly higher than to silibinin and XO inhibition at least as silibinin. PMID:25442301

  1. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties

    PubMed Central

    2013-01-01

    For their various bioactivities, biomaterials derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other diseases. This mini-review comprehensively compares the bioactivities and biological functions of biomaterials from red, green, brown, and blue-green algae. The anti-oxidative effects and bioactivities of several different crude extracts of algae have been evaluated both in vitro and in vivo. Natural products derived from marine algae protect cells by modulating the effects of oxidative stress. Because oxidative stress plays important roles in inflammatory reactions and in carcinogenesis, marine algal natural products have potential for use in anti-cancer and anti-inflammatory drugs. PMID:23724847

  2. Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species.

    PubMed

    El-Hashash, Maher Mahmoud; Abdel-Gawad, Mahfouz Mohamed; El-Sayed, Mortada Mohamed; Sabry, Waffa Ahmed; Abdel-Hameed, El-Sayed Saleh; Abdel-Lateef, Ezzat El-Sayed

    2010-09-01

    In the present study, antioxidant activity of methanolic extracts of the leaves of seven Egyptian Cassia species was investigated using two methods, the phosphomolyb-date method and 1,1 diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity method.· The results revealed that C. glauca is the most potent species and that the activity of other plant species decreases in the following order: C. grandis > C. nodosa > C. fistula > C. didymobtrya > C. occidentalis > C. sophera. Defatted methanolic extract of the most active plant C. glauca was subjected to fractionation using different organic solvents such as CHCl3, EtOAc and BuOH. Antixidant activities of the fractions were investigated and the results showed that ethyl acetate fraction possessed high activity. Total phenolic and flavonoid concentrations of each plant extract were determined using the Folin-Ciocalteu reagent and aluminum chloride. Correlation between radical scavenging capacities of extracts and total phenolic concentration was observed. PMID:21134869

  3. Red Ginseng (Panax ginseng) Decreases Isoproterenol-Induced Cardiac Injury via Antioxidant Properties in Porcine

    PubMed Central

    Lim, Kyu Hee; Cho, Jae Youl; Kim, Bumseok; Bae, Bong-Seuk

    2014-01-01

    Abstract Red ginseng (RG, Panax ginseng) has been shown to possess various ginsenosides. These ginsenosides are widely used for treating cardiovascular diseases in Asian communities. The present study was designed to evaluate the cardioprotective potential of RG against isoproterenol (ISO)-induced myocardial infarction (MI), by assessing electrocardiographic, hemodynamic, and biochemical parameters. Male porcines were orally administered with RG (250 and 500?mg/kg) or with vehicle for 9 days, with concurrent intraperitoneal injections of ISO (20?mg/kg) on the 8th and 9th day. RG significantly attenuated ISO-induced cardiac dysfunctions as evidenced by improved ventricular hemodynamic functions and reduced ST segment and QRS complex intervals. Also, RG significantly ameliorated myocardial injury parameters such as antioxidants. Malonaldialdehyde formation was also inhibited by RG. Based on the results, it is concluded that RG possesses significant cardioprotective potential through the inhibition of oxidative stress and may serve as an adjunct in the treatment and prophylaxis of MI. PMID:24456361

  4. Comparison of phenolic profiles and antioxidant properties of European Fagopyrum esculentum cultivars.

    PubMed

    Kiprovski, Biljana; Mikulic-Petkovsek, Maja; Slatnar, Ana; Veberic, Robert; Stampar, Franci; Malencic, Djordje; Latkovic, Dragana

    2015-10-15

    The purpose of this study was to investigate composition and content of phenolic compounds in seeds of common buckwheat (Fagopyrum esculentum Moench) cultivars from Western, Central and Southeastern Europe grown in the Balkan area, and to compare them with cultivars from the Balkan. Mostly detected hydroxycinnamic acids in seeds of the investigated cultivars were caffeic and chlorogenic acid derivatives. More than ten different flavanols were detected in the investigated seeds, based on which all tested buckwheat cultivars were divided into two groups: those with high propelargonidins (epiafzelechin-epicatechin) and those with high procyanidins contents. 'Novosadska' had the highest level of phenolic acids, proanthocyanidins, flavones and most of the flavonols. However, 'Bosna 1' and 'Bosna 2' were highlighted with the greatest rutin content (up to 46 times higher than in other cultivars). All buckwheat cultivars had quite high antioxidant capacity (more than 80% of neutralized radicals), yet, 'Novosadska', 'Godijevo', 'Spacinska 1' and 'Bamby' excelled. PMID:25952839

  5. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties.

    PubMed

    Lee, Jin-Ching; Hou, Ming-Feng; Huang, Hurng-Wern; Chang, Fang-Rong; Yeh, Chi-Chen; Tang, Jen-Yang; Chang, Hsueh-Wei

    2013-01-01

    For their various bioactivities, biomaterials derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other diseases. This mini-review comprehensively compares the bioactivities and biological functions of biomaterials from red, green, brown, and blue-green algae. The anti-oxidative effects and bioactivities of several different crude extracts of algae have been evaluated both in vitro and in vivo. Natural products derived from marine algae protect cells by modulating the effects of oxidative stress. Because oxidative stress plays important roles in inflammatory reactions and in carcinogenesis, marine algal natural products have potential for use in anti-cancer and anti-inflammatory drugs. PMID:23724847

  6. Enzymatic protein hydrolysates from high pressure-pretreated isolated pea proteins have better antioxidant properties than similar hydrolysates produced from heat pretreatment.

    PubMed

    Girgih, Abraham T; Chao, Dongfang; Lin, Lin; He, Rong; Jung, Stephanie; Aluko, Rotimi E

    2015-12-01

    Isolated pea protein (IPP) dispersions (1%, w/v) were pretreated with high pressure (HP) of 200, 400, or 600MPa for 5min at 24°C or high temperature (HT) for 30min at 100°C prior to hydrolysis with 1% (w/w) Alcalase. HP pretreatment of IPP at 400 and 600MPa levels led to significantly (P<0.05) improved (>40%) oxygen radical absorption capacity (ORAC) of hydrolysates. 2,2-Diphenyl-1-picrylhydrazyl, superoxide radical and hydroxyl radical scavenging activities of pea protein hydrolysates were also significantly (P<0.05) improved (25%, 20%, and 40%, respectively) by HP pretreatment of IPP. Protein hydrolysates from HT IPP showed no ORAC, superoxide or hydroxyl scavenging activity but had significantly (P<0.05) improved (80%) ferric reducing antioxidant power. The protein hydrolysates had weaker antioxidant properties than glutathione but overall, the HP pretreatment was superior to HT pretreatment in facilitating enzymatic release of antioxidant peptides from IPP. PMID:26041225

  7. Properties and characterization of hydrophobized microfibrillated cellulose

    Microsoft Academic Search

    Martin Andresen; Leena-Sisko Johansson; Bjørn Steinar Tanem; Per Stenius

    2006-01-01

    Microfibrillated cellulose (MFC) obtained by disintegration of bleached softwood sulphite pulp in a homogenizer, was hydrophobically modified by surface silylation with chlorodimethyl isopropylsilane (CDMIPS). The silylated MFC was characterized by Fourier transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), transmission electron spectroscopy (TEM), X-ray photoelectron spectroscopy (XPS) and white light interferometry (WLI). The degree of surface substitution (DSS) was determined

  8. Curcumin and its carbocyclic analogs: structure-activity in relation to antioxidant and selected biological properties.

    PubMed

    Bhullar, Khushwant S; Jha, Amitabh; Youssef, Dani; Rupasinghe, H P Vasantha

    2013-01-01

    Curcumin is the major phenolic compound present in turmeric (Curcuma longa L.). Curcumin and 15 novel analogs were investigated for their antioxidant and selected biological activities. Strong relationships between the structure and evaluated activity revealed that the compounds with specific functional groups and carbon skeleton had specific biological profiles. Among the compounds tested, the derivatives (E)-2-(3,4-dimethoxybenzylidene)-5-((E)-3-(3,4-dimethoxyphenyl)acryloyl)cyclopentanone (3e), and (E)-2-(4-hydroxy-3-methoxybenzylidene)-5-((E)-3-(4-hydroxy-3-methoxyphenyl)acryloyl)-cyclopentanone (3d) and the parent compound curcumin exhibited the strongest free radical scavenging and antioxidant capacity. Concerning the other biological activities studied the compound (E)-2-(4-hydroxy-3-methoxybenzylidene)-5-((E)-3-(4-hydroxy-3-methoxy-phenyl)-acryloyl)cyclopentanone (3d) was the most potent angiotensin converting enzyme (ACE) inhibitor, while the derivatives (E)-2-(4-hydroxybenzylidene)-6-((E)-3-(4-hydroxyphenyl)acryloyl)cyclohexanone (2b), (E)-2-(3,4-dimethoxybenzylidene)-6-((E)-3-(3,4-dimethoxyphenyl)acryloyl)cyclohexanone (2e) and (E)-2-(3,4-dimethoxybenzylidene)-5-((E)-3-(3,4-dimethoxyphenyl)acryloyl)cyclopentanone (3e) exhibited strong tyrosinase inhibition. Moreover, (E)-2-(3,4-dimethoxybenzylidene)-6-((E)-3-(3,4-dimethoxyphenyl)-acryloyl)cyclohexanone (2e) was also found to be the strongest human HIV-1 protease inhibitor in vitro among the tested compounds. Cytotoxicity studies using normal human lung cells revealed that the novel curcumin as well as its carbocyclic analogs are not toxic. PMID:23666006

  9. Attenuation of myocardial ischemia-reperfusion injury by trimetazidine derivatives functionalized with antioxidant properties.

    PubMed

    Kutala, Vijay Kumar; Khan, Mahmood; Mandal, Rajarsi; Ganesan, Latha P; Tridandapani, Susheela; Kalai, Tamas; Hideg, Kalman; Kuppusamy, Periannan

    2006-06-01

    Trimetazidine (TMZ), an anti-ischemic metabolic drug, is used to treat chest pain (angina pectoris). We hypothesized that derivatives of TMZ with antioxidant functions may improve the cardiac dysfunction caused by ischemia-reperfusion (I/R) above that observed with TMZ alone. Isolated rat hearts perfused with Krebs-Henseleit buffer according to the Langendorff method were subjected to 30 min of global ischemia followed by 45 min of reperfusion. Trimetazidine, TMZ-NH (TMZ modified with a pyrroline moiety), or TMZ-PhiNH (TMZ-NH with a phenyl substitute) were infused (50 microM) for 1 min before the onset of ischemia. Untreated (control) hearts at the end of 45 min of reperfusion showed a significant decrease in the recovery of coronary flow (42%), left ventricular-developed pressure (22%), and rate-pressure product (25%) compared with preischemic baseline values. The I/R hearts also showed markedly increased lactate dehydrogenase and creatine kinase activities in the coronary effluent, significant myocardial infarction (46% of risk area), and activation of Akt, extracellular signal-regulated kinase, and p38 mitogen-activated protein kinase. Pretreatment of hearts with TMZ-NH or TMZ-PhiNH significantly enhanced the recovery of heart function and decreased infarct size. The I/R-induced activation of Akt was further enhanced by TMZ-PhiNH. The present study demonstrated that TMZ-NH and TMZ-PhiNH significantly protected hearts against I/R-mediated cardiac dysfunction and injury. The protective effect of the TMZ derivatives could be due to the combined effects of antioxidant and anti-ischemic activities as well as enhanced pro-survival Akt activity. PMID:16467453

  10. Synthesis, characterization, X-ray crystallography, acetyl cholinesterase inhibition and antioxidant activities of some novel ketone derivatives of gallic hydrazide-derived Schiff bases.

    PubMed

    Gwaram, Nura Suleiman; Ali, Hapipah Mohd; Abdulla, Mahmood Ameen; Buckle, Michael J C; Sukumaran, Sri Devi; Chung, Lip Yong; Othman, Rozana; Alhadi, Abeer A; Yehye, Wageeh A; Hadi, A Hamid A; Hassandarvish, Pouya; Khaledi, Hamid; Abdelwahab, Siddig Ibrahim

    2012-01-01

    Alzheimer's disease (AD) is the most common form of dementia among older people and the pathogenesis of this disease is associated with oxidative stress. Acetylcholinesterase inhibitors with antioxidant activities are considered potential treatments for AD. Some novel ketone derivatives of gallic hydrazide-derived Schiff bases were synthesized and examined for their antioxidant activities and in vitro and in silico acetyl cholinesterase inhibition. The compounds were characterized using spectroscopy and X-ray crystallography. The ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays revealed that all the compounds have strong antioxidant activities. N-(1-(5-bromo-2-hydroxyphenyl)-ethylidene)-3,4,5-trihydroxybenzohydrazide (2) was the most potent inhibitor of human acetyl cholinesterase, giving an inhibition rate of 77% at 100 ?M. Molecular docking simulation of the ligand-enzyme complex suggested that the ligand may be positioned in the enzyme's active-site gorge, interacting with residues in the peripheral anionic subsite (PAS) and acyl binding pocket (ABP). The current work warrants further preclinical studies to assess the potential for these novel compounds for the treatment of AD. PMID:22374313

  11. Chemical composition and antioxidant property of holy basil (Ocimum sanctum L.) leaves, stems, and inflorescence and their in vitro callus cultures.

    PubMed

    Hakkim, F Lukmanul; Shankar, C Gowri; Girija, S

    2007-10-31

    In this study, the chemical constituents and antioxidant property of holy basil (Ocimum sanctum Linn.) field-grown plant parts (leaves, stems, and inflorescence) were compared with those of respective callus cultures induced from each explant in in vitro. The callus cultures were successfully initiated on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (1 mg/L) combined with different concentrations (0.1-0.5 mg/L) of kinetin as plant growth regulators. The distribution of phenolic compounds in these extracts was analyzed using reverse phase high-performance liquid chromatography with reference standards. Interestingly, rosmarinic acid (RA) was found to be the predominant phenolic acid in all callus extracts in comparison with field-grown plant parts. In this study, the antioxidant activity of the extracts was evaluated with six different in vitro antioxidant-testing systems. Their activities of scavenging superoxide anion radicals, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), hydroxyl radicals, hydrogen peroxide, chelating ferrous iron, and ferric ion reducing potential were assessed. The antioxidant activity was increased in all testing systems with increasing amounts of extract. However, at the same concentration, the callus extracts exhibited higher antioxidant activity in all of the testing systems than the extract obtained from field-grown plant parts. The data obtained from this study suggested the possibility of the isolation of a high content of RA from in vitro callus cultures rather than field-grown plant organs of holy basil. PMID:17924700

  12. Influence of melatonin supplementation on serum antioxidative properties and impact of the quality of life in multiple sclerosis patients.

    PubMed

    Adamczyk-Sowa, M; Pierzchala, K; Sowa, P; Polaniak, R; Kukla, M; Hartel, M

    2014-08-01

    The relationship between the prevalence of multiple sclerosis (MS) and sunlight's ultraviolet radiation was proved. Oxidative stress plays a role in the pathogenic traits of MS. Melatonin possesses antioxidative properties and regulates circadian rhythms. Several studies have reported that the quality of life is worse in patients with MS than in healthy controls, with a higher prevalence of sleep disturbances, depression and fatigue. The aim of study was to evaluate 5 mg daily melatonin supplementation over 90 days on serum malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity and its' influence on impact of the quality of life of MS patients. A case-control prospective study was performed on 102 MS patients and 20 controls matched for age and sex. The EDSS, MRI examinations and Multiple Sclerosis Impact Scale (MSIS-29) questionnaire was completed. Marked increase in serum MDA concentration in all MS patients groups was observed and after melatonin treatment decreased significantly in interferons-beta and glatiramer acetate-treated groups, but not in mitoxantrone-treated group. A significant increase in SOD activity compared to controls only in glatiramer acetate-treated group was observed. After 3 months melatonin supplementation the SOD activity increased compared to initial values in interferons beta-treated groups. A significant increase in both MSIS-29-PHYS and MSIS-29-PSYCH items mean scores only in the MX group as compared to other groups was observed. There were no significant differences in mean MSIS-29-PHYS was observed before and after melatonin therapy. Melatonin supplementation caused a decrease in mean MSIS-29-PSYCH scores compared to initial values in interferons beta-treated groups. Finding from our study suggest that melatonin can act as an antioxidant and improves reduced quality in MS patients. PMID:25179086

  13. Characterization of the Electrical Properties of Polycrystalline Diamond Films

    NASA Astrophysics Data System (ADS)

    Zhao, Shulai

    1994-01-01

    The electrical properties of chemical vapor deposited (CVD) diamond films have been measured using charged particle -induced conductivity (CPIC). The collection distance, d, that a carrier drifts under the influence of an applied electric field was measured. In terms of the electrical properties, d = mu Etau where mu is the carrier mobility and tau is the excess carrier lifetime. It was found that there is a constant gradient of collection distance along the film growth direction for the CVD diamond films produced by various techniques. Material characterizations were performed on the CVD diamond films. Visual observation, scanning electron microscopy, x-ray diffraction, Raman spectroscopy and photoluminescence were used to investigate the limitations of the electrical properties. Correlations were found between electrical property studies and the material characterizations. Crystal structural defects were found to be the factor limiting the electrical properties in CVD diamond.

  14. Composite propellant technology research: Mechanical property characterization

    NASA Technical Reports Server (NTRS)

    Bower, Mark V.

    1991-01-01

    Proof for the existence of a single Poisson's ratio function in isotropic linear viscoelastic materials is presented. An in-depth discussion is given of three dimensional viscoelastic material properties and their relationships to linear isotropic and orthotropic viscoelastic materials. A discussion of the alternate invariant definition as used by Abaqus and how it relates to the form used by Dr. S. Peng is presented.

  15. Rheological innovations for characterizing food material properties.

    PubMed

    Melito, H S; Daubert, C R

    2011-01-01

    Rheological methods are continually evolving to encompass novel technologies and measurement methods. This review highlights novel techniques used to analyze the rheological properties of foods over the previous decade. Techniques reviewed include large amplitude oscillatory shear (LAOS) testing and rheological techniques coupled with other measurement methods, such as microscopy and nuclear magnetic resonance (NMR). Novel techniques are briefly overviewed and discussed in terms of advantages and disadvantages, previous use, and suggested future utilization. PMID:22129379

  16. Microstructure characterization and magnetic properties of nanomaterials

    Microsoft Academic Search

    Xiang-Cheng Sun

    2002-01-01

    The microstructure and superparamagnetic properties of two systems of magnetic nanoparticles are reviewed. A new type of magnetic core-shell Ni-Ce nanocomposite particle (15-50nm) has been prepared. Typical HREM images and FFT patterns of HREM images showed that many planar defects (nanotwins and stacking faults) exist in the large Ni core zone (10-45nm). The shell layers (3-5nm) consist of an innermost

  17. NMR Characterizations of Properties of Heterogeneous Media

    SciTech Connect

    Watson, A. Ted; Phan, Jack; Uh, Jinsoo; Michalak, Rudi; Xue, Song

    2003-01-28

    The overall goal of this project was to develop reliable methods for resolving macroscopic properties important for describing the flow of one or more fluid phases in reservoirs from formation measurements. Completed the facilities to house our new NMR imager, the equipment has been delivered and installed. New experimental designs will provide for more reliable estimation of permeability distributions were evaluated. Designed and built a new core holder to incorporate one of the new experimental designs.

  18. Silicon and germanium nanocrystals: properties and characterization

    PubMed Central

    Carvalho, Alexandra; Coutinho, José

    2014-01-01

    Summary Group-IV nanocrystals have emerged as a promising group of materials that extends the realm of application of bulk diamond, silicon, germanium and related materials beyond their traditional boundaries. Over the last two decades of research, their potential for application in areas such as optoelectronic applications and memory devices has been progressively unraveled. Nevertheless, new challenges with no parallel in the respective bulk material counterparts have arisen. In this review, we consider what has been achieved and what are the current limitations with regard to growth, characterization and modeling of silicon and germanium nanocrystals and related materials. PMID:25383290

  19. Antioxidant and Anti-Inflammatory Properties of Solid-State Fermented Products from a Medicinal Mushroom, Taiwanofungus salmoneus (Higher Basidiomycetes) from Taiwan.

    PubMed

    Chiang, Shen-Shih; Ulziijargal, Enkhjargal; Chien, Rao-Chi; Mau, Jeng-Leun

    2015-01-01

    Cooked grains of buckwheat (TSFB), oats (TSFO), embryo rice (TSFR), or wheat (TSFW) were inoculated with the medicinal fungus Taiwanofungus salmoneus (=Antrodia salmonea), and the metabolites formed were evaluated for antioxidant activity using the conjugated diene method, reducing power, scavenging ability, and chelating ability. Overall, the effectiveness was generally in the descending order of TSFB ? TSFO > TSFR ? TSFW. The correlation between effective concentration (EC50) of antioxidant property and antioxidant component content measured was established. However, flavonoid contents correlated well with antioxidant properties assayed (r = 0.707-0.933). Fermented products contained substantial amounts of adenosine (34.7-73.8 µg/g), ergosterol (1070-1212 µg/g), ergothioneine (81.0-119.8 µg/g), ?-aminobutyric acid (100.9-170.2 µg/g), and lovastatin (3.06-5.60 µg/g). In addition, with the addition of the extracts from fermented products, lipopolysaccharide-induced nitric oxide, tumor necrosis factor-?, interleukin-1?, and interleukin-6 production in RAW 264.7 murine macrophage cells could be effectively suppressed. Accordingly, four types of T. salmoneus fermented products could be beneficially used as food-flavoring materials and food ingredients or as nutritional supplements. PMID:25746403

  20. Antioxidant properties and phenolic profile of the most widely appreciated cultivated mushrooms: a comparative study between in vivo and in vitro samples.

    PubMed

    Reis, Filipa S; Martins, Anabela; Barros, Lillian; Ferreira, Isabel C F R

    2012-05-01

    The present study reports a comparison of the antioxidant properties and phenolic profile of the most consumed species as fresh cultivated mushrooms and their mycelia produced in vitro: Agaricus bisporus (white and brown), Pleurotus ostreatus (oyster), Pleurotus eryngii (king oyster) and Lentinula edodes (shiitake). The antioxidant activity was evaluated through reducing power (Folin-Ciocalteu and Ferricyanide/Prussian blue assays), free radical scavenging activity (DPPH assay) and lipid peroxidation inhibition (?-carotene/linoleate and TBARS assays). The analysis of phenolic compounds was performed by HPLC/PAD. The mushroom species with the highest antioxidant potential was Agaricus bispous (brown). However, concerning to the species obtained in vitro, it was L. edodes that demonstrate the highest reducing power. Generally, in vivo samples revealed higher antioxidant properties than their mycelia obtained by in vitro techniques. About the phenolic compounds researched, they were detected both in mushrooms and mycelia without any particular abundance. Results showed that there is no correlation between the studied commercial mushrooms and the corresponding mycelia obtained in vitro. Nevertheless, this study contributes to the rise of data relatively to the species consumed as fresh mushrooms and the possibility of their in vitro production as a source of bioactive compounds. PMID:22369965

  1. Teucrium Plant Species as Natural Sources of Novel Anticancer Compounds: Antiproliferative, Proapoptotic and Antioxidant Properties

    PubMed Central

    Stankovic, Milan S.; Curcic, Milena G.; Zizic, Jovana B.; Topuzovic, Marina D.; Solujic, Slavica R.; Markovic, Snezana D.

    2011-01-01

    This study deals with total phenolic content, antiproliferative and proapoptotic activity of methanolic extracts from different Teucrium species and the effect on the prooxidant/antioxidant status in HCT-116 cells. The total phenolic content of the extracts was measured spectrophotometricaly and the obtained results ranged from 56.62 mg/g to 172.50 mg GA/g. The antiproliferative activity of methanolic extracts from different Teucrium species was determined using MTT cell viability assay, where IC50 value was used as a parameter for cytotoxicity. The type of cell death was explored by fluorescence microscopy using the acridin orange/ethidium bromide method. MTT assay showed that all extracts significantly reduced cell viability in a dose-dependent manner, with very low IC50 values. The highest content of phenolic compounds and the best cytotoxic activity on HCT-116 cells after 24 h of exposure was in T. chamaedrys extract, with IC50 values of 5.48 × 10?9 ?g/mL. After 72 h, methanolic extract of T. arduini appeared to have the best cytotoxic activity on HCT-116, with IC50 values of 0.37 ?g/mL. Treatments caused typical apoptotic morphological changes in HCT-116 cells and showed a high percentage of apoptotic cells. The results of the presented research indicate that some Teucrium extracts are a very rich source of phenols, which may directly contribute to high antiproliferative and proapoptotic activity. PMID:21845072

  2. Ozone Quenching Properties of Isoprene and Its Antioxidant Role in Leaves1

    PubMed Central

    Loreto, Francesco; Mannozzi, Michela; Maris, Christophe; Nascetti, Pamela; Ferranti, Francesco; Pasqualini, Stefania

    2001-01-01

    Isoprene is formed in and emitted by plants and the reason for this apparent carbon waste is still unclear. It has been proposed that isoprene stabilizes cell and particularly chloroplast thylakoid membranes. We tested if membrane stabilization or isoprene reactivity with ozone induces protection against acute ozone exposures. The reduction of visible, physiological, anatomical, and ultrastructural (chloroplast) damage shows that clones of plants sensitive to ozone and unable to emit isoprene become resistant to acute and short exposure to ozone if they are fumigated with exogenous isoprene, and that isoprene-emitting plants that are sensitive to ozone do not suffer damage when exposed to ozone. Isoprene-induced ozone resistance is associated with the maintenance of photochemical efficiency and with a low energy dissipation, as indicated by fluorescence quenching. This suggests that isoprene effectively stabilizes thylakoid membranes. However, when isoprene reacts with ozone within the leaves or in a humid atmosphere, it quenches the ozone concentration to levels that are less or non-toxic for plants. Thus, protection from ozone in plants fumigated with isoprene may be due to a direct ozone quenching rather than to an induced resistance at membrane level. Irrespective of the mechanism, isoprene is one of the most effective antioxidants in plants. PMID:11457950

  3. Mucilage chemical profile and antioxidant properties of giant swamp taro tubers.

    PubMed

    Nguimbou, Richard Marcel; Boudjeko, Thaddée; Njintang, Nicolas Yanou; Himeda, Makhlouf; Scher, Joël; Mbofung, Carl M F

    2014-12-01

    The yellow (YP) and white (WP) sections of giant swamp taro (GST) contain 40.0 g/kg and 51.5 g/kg (dry wt) respectively of pure mucilage made up of D-glucose (44.95-78.85 %), D-galactose (8.70-25.35 %), D-mannose (3.20-10.45 %), D-arabinose (2.45-5.20 %) and small amounts of glucuronic acid and rhamnose. Arabinogalactan-proteins (5.30-8.83 g/kg) contain mainly arabinose and galactose (in a 1:1 proportion) and also significant amounts of rhamnose, xylose, glucuronic acid and mannose. Antioxidant activity of YP was higher than those of WP while chelating ability and reducing power increased with mucilages content. Generally YP and WP showed better reducing power (1.06?±?0.35 at 5 mg/mL) than ascorbic acid (0.89?±?0.22). WP and YP chelated ferrous ions by 20.0-76.0 % and 16.4-71.0 % respectively. Effective concentrations (EC50) of mucilages (WP 1.28?±?0.05 mg/mL; YP 1.42?±?0.04 mg/mL) were lower than those of citric acid (1.58?±?0.04 mg/mL). Generally mucilage from the WP and YP sections are excellent sources of chelating agents. PMID:25477624

  4. Antihyperglycaemic and anti-oxidant properties of Anoectochilus formosanus in diabetic rats.

    PubMed

    Shih, Chun-Ching; Wu, Yueh-Wern; Lin, Wen-Chuan

    2002-08-01

    1. In the present study, we investigated aqueous extracts of Anoectochilus formosanus (AFE) for antihyperglycaemic and anti-oxidant effects in diabetic rats induced by streptozotocin (STZ). 2. Diabetic rats were randomly divided into groups and treated orally by gavage with vehicle (distilled water) or AFE (1 and 2 g/kg), once a day for 21 days. 3. At the end of the 21 day period, AFE (2 g/kg) significantly reduced fasting blood glucose, serum fructosamine, triglycerides and total cholesterol compared with vehicle-treated diabetic rats. In vehicle-treated diabetic rats, levels of renal lipid peroxidation were increased, whereas glutathione concentrations were not affected. Renal lipid peroxidation levels were significantly lower and renal reduced glutathione (GSH) concentrations were significantly higher in AFE-treated diabetic rats compared with vehicle-treated diabetic rats. The diabetic kidney in the vehicle-treated group showed a decrease in catalase, but the activity of glutathione peroxidase (GSH-Px) was increased. 4. The activity of catalase, but not GSH-Px, was significantly reversed by AFE treatment. These results indicate that AFE (1 and 2 g/kg) not only possesses an antihyperglycemic effect, but that it may also reduce oxidative stress in diabetic rats. PMID:12100000

  5. Physio-mechanical properties of an active chitosan film incorporated with montmorillonite and natural antioxidants extracted from pomegranate rind.

    PubMed

    Qin, Yu-Yue; Zhang, Zhi-Hong; Li, Lin; Yuan, Ming-Long; Fan, Jian; Zhao, Tian-Rui

    2015-03-01

    An active film was prepared from chitosan incorporated with montmorillonite (MMT) and pomegranate rind powder extract (PRP). The effect of MMT (1 %, 3 %, and 5 % w/w chitosan) and PRP (1 %, 1.5 %, and 2 % w/v chitosan) on the physical, mechanical and antioxidant properties of the chitosan-based films was studied. Fourier transform infrared (FTIR) spectra revealed that good interactions occurred between functional groups of chitosan with MMT or with PRP. The results showed that the water vapor barrier property of the films was significantly improved by incorporation of MMT and PRP (p?

  6. NMR Characterizations of Properties of Heterogeneous Media

    SciTech Connect

    Uh, Jinsoo; Phan, Jack; Xue, Dong; Watson, A. Ted

    2003-01-28

    The overall goal of this project was to develop reliable methods for resolving macroscopic properties important for describing the flow of one or more fluid phases in reservoirs from formation measurements. During this reporting period, the determination of surface relaxivity from NMR data was investigated. A new method for determining the surface relaxivity from measured data was developed and tested with data obtained from an Exxon sample. The new method avoids the use of a certain mathematical short-time approximation in the data analysis, which has been shown to be unsuitable.

  7. Synthesis, Characterization, Antioxidant Status, and Toxicity Study of Vanadium-Rutin Complex in Balb/c Mice.

    PubMed

    Roy, Souvik; Majumdar, Sumana; Singh, Amit Kumar; Ghosh, Balaram; Ghosh, Nilanjan; Manna, Subhadip; Chakraborty, Tania; Mallick, Sougato

    2015-08-01

    A new trend was developed for the formation of a complex between vanadium and flavonoid derivatives in order to increase the intestinal absorption and to reduce the toxicity of vanadium compounds. The vanadium-rutin complex was characterized by several spectroscopic techniques like ultraviolet (UV)-visible, Fourier transform infrared (FTIR), NMR, mass spectrometry, and microscopic evaluation by scanning electron microscopy. The mononuclear complex was formed by the interaction between vanadium and rutin with 1:2 metal to ligand stoichiometry. Antioxidant activity of the complex was evaluated by 1,1-diphenyl-2 picrylhydrazyl, ferric-reducing power, and 2,2'-azin-obis 3-ethylbenzothiazoline-6-sulphonic acid methods. It was shown that radical scavenging activity and ferric-reducing potential of free rutin was lower as compared with vanadium-rutin complex. The study was also investigated for oral acute toxicity and 28 days repeated oral subacute toxicity study of vanadium-rutin complex in balb/c mice. The vanadium-rutin complex showed mortality at a dose of 120 mg/kg in the balb/c mice. In 28 days repeated oral toxicity study, vanadium-rutin complex was administered to both sex of balb/c mice at dose levels of 90, 45, and 20 ppm, respectively. In addition, subacute toxicity study of vanadium-rutin complex (at 90 ppm dose level) showed increase levels of white blood cell (WBC), total bilirubin, alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatinine, and blood urea nitrogen and decrease level of total protein (TP) as compared with control group. Histopathological study of vanadium-rutin showed structural alteration in the liver, kidney, and stomach at 90 ppm dose level. No observed toxic level of vanadium-rutin complex at 20 ppm dose level could be good for further study. PMID:25697629

  8. Effective inhibition of skin cancer, tyrosinase, and antioxidative properties by astaxanthin and astaxanthin esters from the green alga Haematococcus pluvialis.

    PubMed

    Rao, Ambati Ranga; Sindhuja, H N; Dharmesh, Shylaja M; Sankar, Kadimi Udaya; Sarada, Ravi; Ravishankar, Gokare Aswathanarayana

    2013-04-24

    Astaxanthin mono- (AXME) and diesters (AXDE) were characterized and examined for anticancer potency with total carotenoids (TC) and astaxanthin (AX) against UV-7,12-dimethylbenz(a)anthracene (DMBA)-induced skin cancer model in rat. At 200 ?g/kg bw, AXDE and AXME reduced UV-DMBA-induced tumor incidences up to 96 and 88%, respectively, when compared to AX (66%) and TC (85%). UV-DMBA has been known to generate high levels of free radicals and tyrosinase enzyme, leading to characteristic symptoms of skin pigmentation and tumor initiation. Intriguingly, ~7-fold increase in tyrosinase and 10-fold decrease in antioxidant levels were normalized by AXDE and AXME as opposed to only ~1.4-2.2-fold by AX and TC, respectively. This result together with the appearance of 72 and 58 ng/mL of retinol in the serum of respective AXE-treated (AXDE + AXME) and AX-treated animals suggested that better anticancer potency of AXEs could be due to increased bioavailability. PMID:23473626

  9. Molecular characterization of Clonorchis sinensis secretory myoglobin: Delineating its role in anti-oxidative survival

    PubMed Central

    2014-01-01

    Background Clonorchiasis is a globally important, neglected food-borne disease caused by Clonorchis sinensis (C. sinensis), and it is highly related to cholangiocarcinoma and hepatocellular carcinoma. Increased molecular evidence has strongly suggested that the adult worm of C. sinensis continuously releases excretory-secretory proteins (ESPs), which play important roles in the parasite-host interactions, to establish successful infection and ensure its own survival. Myoglobin, a hemoprotein, is present in high concentrations in trematodes and ESPs. To further understand the biological function of CsMb and its putative roles in the interactions of C. sinensis with its host, we explored the molecular characterization of CsMb in this paper. Methods We expressed CsMb and its mutants in E. coli BL21 and identified its molecular characteristics using bioinformatics analysis and experimental approaches. Reverse transcription PCR analysis was used to measure myoglobin transcripts of C. sinensis with different culture conditions. The peroxidase activity of CsMb was confirmed by spectrophotometry. We co-cultured RAW264.7 cells with recombinant CsMb (rCsMb), and we then measured the production of hydrogen peroxide (H2O2) and nitric oxide (NO) in addition to the mRNA levels of inducible nitric oxide synthase (iNOS), Cu-Zn superoxide dismutase (SOD1) and Mn superoxide dismutase (SOD2) in activated RAW264.7 cells. Results In the in vitro culture of adult worms, the transcripts of CsMb increased with the increase of oxygen content. Oxidative stress conditions induced by H2O2 increased the levels of CsMb transcripts in a dose-dependent manner. Furthermore, CsMb catalyzed oxidation reactions in the presence of H2O2, and amino acid 34 of CsMb played an essential role in its reaction with H2O2. In addition, CsMb significantly reduced H2O2 and NO levels in LPS-activated macrophages, and CsMb downregulated iNOS and SOD expression in activated macrophages. Conclusion The present study is the first to investigate the peroxidase activity of CsMb. This investigation suggested that C. sinensis may decrease the redox activation of macrophages by CsMb expression to evade host immune responses. These studies contribute to a better understanding of the role of CsMb in the molecular mechanisms involved in ROS detoxification by C. sinensis. PMID:24885788

  10. Doped silicon carbonitride: Synthesis, characterization and properties

    SciTech Connect

    Bill, J.; Friess, M.; Aldinger, F. [Max-Planck-Inst. fuer Metallforschung, Stuttgart (Germany). Pulvermetallurgisches Laboratorium; Riedel, R. [TH Darmstadt (Germany). Fachbereich Materialwissenschaft

    1994-12-31

    The authors report on an alternative route to quaternary ceramics which can be prepared by polycocondensation of commercially available polysilazanes with the appropriate alkyl amides (M(NRR{prime}){sub x}) with M = B or P and subsequent pyrolysis in argon atmosphere at 1,000 C. Ceramic monoliths are obtained if the modified polysilazane is crosslinked at 400 C for 3 h. After ball-milling, sieving and cold isostatic pressing the green body is directly pyrolyzed to the appropriate monolith. The obtained material is amorphous to X-ray-diffraction. Investigations by electron spectroscopic imaging (ESI) in TEM indicate a homogeneous distribution of the elements M, Si, C and N (M = B, P) within a resolution of 2 nm. Upon annealing at elevated temperatures in a nitrogen atmosphere crystallization of the appropriate thermodynamically stable phases occurs around 1,400 C yielding a micro/nano-, nano/nano- or micro/microcrystalline microstructure with design-tailored properties depending on the element M.

  11. Zeaxanthin production by novel marine isolates from coastal sand of India and its antioxidant properties.

    PubMed

    Prabhu, Sudharshan; Rekha, P D; Young, Chiu-Chung; Hameed, Asif; Lin, Shih-Yao; Arun, A B

    2013-10-01

    Zeaxanthin carotenoids are class of commercially important natural products and diverse biomolecules produced by plants and many microorganisms. Bacteria often produce a cocktail of polar and nonpolar carotenoids limiting their industrial applications. Marine members of the family Flavobacteriaceae are known to produce potential carotenoids such as astaxanthin and zeaxanthin. A few bacterial species have been reported for the predominant production zeaxanthin. Here, we report the molecular identification of the zeaxanthin as a major carotenoid produced by two novel bacteria (YUAB-SO-11 and YUAB-SO-45) isolated from sandy beaches of South West Coast of India and the effect of carbon sources on the production of zeaxanthin. The strains were identified based on the 16S rRNA gene sequencing as a member of genus Muricauda. The closest relatives of YUAB-SO-11 and YUAB-SO-45 were Muricauda aquimarina (JCM 11811(T)) (98.9 %) and Muricauda olearia (JCM 15563(T)) (99.2 %), respectively, indicating that both of these strains might represent a novel species. The highest level of zeaxanthin production was achieved (YUAB-SO-11, 1.20 ± 0.11 mg g(-1)) and (YUAB-SO-45, 1.02 ± 0.13 mg g(-1)) when cultivated in marine broth supplemented with 2 % NaCl (pH 7) and incubated at 30 °C. Addition of 0.1 M glutamic acid, an intermediate of citric acid cycle, enhanced the zeaxanthin production as 18 and 14 % by the strains YUAB-SO-11 and YUAB-SO-45 respectively. The zeaxanthin showed in vitro nitric oxide scavenging, inhibition of lipid peroxidation, and 2,2-diphenyl-1-picryl hydrazyl scavenging activities higher than the commercial zeaxanthin. The results of this study suggest that two novel strains YUAB-SO-11 and YUAB-SO-45 belonging to genus Muricauda produce zeaxanthin as a predominant carotenoid, and higher production of zeaxanthin was achieved on glutamic acid supplementation. The pigment showed good in vitro antioxidant activity, which can be exploited further for commercial applications. PMID:23900617

  12. Impact of high intensity pulsed electric field on antioxidant properties and quality parameters of a fruit juice–soymilk beverage in chilled storage

    Microsoft Academic Search

    M. Morales-de la Peña; L. Salvia-Trujillo; M. A. Rojas-Graü; O. Martín-Belloso

    2010-01-01

    The effects of high-intensity pulsed electric fields (HIPEF) treatment (35 kV\\/cm, 4 ?s bipolar pulses at 200 Hz for 800 or 1400 ?s) on the microbial stability, quality parameters and antioxidant properties of a fruit juice–soymilk (FJ–SM) beverage along the storage time at 4 °C were compared to those obtained by thermal pasteurization (90 °C, 60 s). HIPEF processing for 800 ?s ensured the microbial stability of the

  13. Antioxidant properties of alpha-lipoic acid: effects on red blood membrane permeability and adaptation of isolated rat heart to reversible ischemia

    Microsoft Academic Search

    S. Ghibu; B. Lauzier; S. Delemasure; S. Amoureux; P. Sicard; C. Vergely; A. Muresan; C. Mogosan; L. Rochette

    2009-01-01

    The aim of our work was to study (1) the antioxidant properties of lipoic acid (LA) and its reduced metabolite dihydrolipoic\\u000a acid (DHLA) formed by reduction of LA and (2) the effects of treatment with LA and DHLA on (a) K+ efflux from human red blood cells and (b) post-ischemic recovery and oxidative stress in isolated perfused rat hearts challenged

  14. Ultrasound-assisted extraction methodology as a tool to improve the antioxidant properties of herbal drug Xiao-chia-hu-tang

    Microsoft Academic Search

    Chu-Ting Liu; Ching-Yi Wu; Yih-Ming Weng; Chin-Yin Tseng

    2005-01-01

    Xiao-chai-hu-tang (XCHT) is an important Chinese herbal prescription for curing many types of liver diseases. The contents of bioactive constituents (saikosaponins a, c and d, baicalin, baicalein, and glycyrrhizic acid), and antioxidant properties of XCHT extracts prepared with ultrasound-assisted (US) extraction in combination with ethanol (up to 95%) as extraction modifier were studied. The results showed that the US extraction

  15. Influence of the preparation temperature on the phase, microstructure and anti-oxidation property of a SiC coating for C\\/C composites

    Microsoft Academic Search

    Huang Jian-Feng; Zeng Xie-Rong; Li He-Jun; Xiong Xin-Bo; Fu Ye-wei

    2004-01-01

    A SiC coating for carbon\\/carbon(C\\/C) composites was produced by a pack cementation technique in an argon atmosphere. The phase, microstructure and anti-oxidation property of the SiC coatings prepared from 1773 to 2273 K were investigated. It is found that the porous ?-SiC coatings occur at 1773–1973 K while dense Si\\/?-SiC coatings generate at 2073 K and micro-cracked ?-SiC\\/?-SiC coatings appear

  16. Investigation of Antidiabetic, Antihyperlipidemic, and In Vivo Antioxidant Properties of Sphaeranthus indicus Linn. in Type 1 Diabetic Rats: An Identification of Possible Biomarkers

    PubMed Central

    Ramachandran, S.; Asokkumar, K.; Uma Maheswari, M.; Ravi, T. K.; Sivashanmugam, A. T.; Saravanan, S.; Rajasekaran, A.; Dharman, J.

    2011-01-01

    The present investigation was aimed to study the antidiabetic, antihyperlipidemic, and in vivo antioxidant properties of the root of Sphaeranthus indicus Linn. in streptozotocin- (STZ-) induced type 1 diabetic rats. Administration of ethanolic extract of Sphaeranthus indicus root (EESIR) 100 and 200?mg/kg to the STZ-induced diabetic rats showed significant (P < .01) reduction in blood glucose and increase in body weight compared to diabetic control rats. Both the doses of EESIR-treated diabetic rats showed significant (P < .01) alteration in elevated lipid profile levels than diabetic control rats. The EESIR treatment in diabetic rats produced significant increase in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and decrease in thiobarbituric acid reactive substances (TBARS) levels than diabetic control rats. Administration of EESIR 200?mg/kg produced significant (P < .01) higher antioxidant activity than EESIR 100?mg/kg. The high performance liquid chromatography (HPLC) analysis of EESIR revealed the presence of biomarkers gallic acid and quercetin. In conclusion, EESIR possess antidiabetic, antihyperlipidemic, and in vivo antioxidant activity in type 1 diabetic rats. Its antioxidant and lipid lowering effect will help to prevent diabetic complications, and these actions are possibly due to presence of above biomarkers. PMID:20953435

  17. The effect of extrusion conditions on the acidic polysaccharide, ginsenoside contents and antioxidant properties of extruded Korean red ginseng

    PubMed Central

    Gui, Ying; Ryu, Gi Hyung

    2013-01-01

    This study was conducted to investigate the effect of extrusion conditions (moisture content 20% and 30%, screw speed 200 and 250 rpm, barrel temperature 115? and 130?) on the acidic polysaccharide, ginsenoside contents and antioxidant properties of extruded Korean red ginseng (KRG). Extruded KRGs showed relatively higher amounts of acidic polysaccharide (6.80% to 9.34%) than nonextruded KRG (4.34%). Increased barrel temperature and screw speed significantly increased the content of acidic polysaccharide. The major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg2s, Rg3s, Rh1, and Rg3r) of KRG increased through extrusion, while the ginsenoside (Rg1) decreased. The EX8 (moisture 30%, screw speed 250 rpm, and temperature 130?) had more total phenolics and had a better scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radicals than those of extruded KRG samples. The extrusion cooking showed a significant increase (6.8% to 20.9%) in reducing power. Increased barrel temperature significantly increased the values of reducing power, the highest value was 1.152 obtained from EX4 (feed moisture 20%, screw speed 250 rpm, and temperature 130?). These results suggest that extrusion conditions can be optimized to retain the health promoting compounds in KRG products. PMID:23717175

  18. Evaluation of antioxidant properties, elemental and phenolic contents composition of wild nettle (Urtica dioica L.) from Tunceli in Turkey.

    PubMed

    Yildirim, N C; Turkoglu, S; Ince, O K; Ince, M

    2013-01-01

    Wild nettle (Urtica dioica L.) types were sampled from different geographical regions in Tunceli (Turkey) to determine their mineral, vitamin, phenolic contents and their antioxidant properties. The total phenol varied from 37.419 ± 0.380 to 19.182 ± 1.00 mg of GAEs g(-1) of dry nettle. The highest radical scavenging effect was observed in Mazgirt parting of the ways 7.5 km with 33.70 ± 0.849 mg mL(-1). The highest reducing power was observed in the nettles from Mazgirt parting of the ways 7.5 km. Among the various macronutrients estimated in the plant samples, potassium was present in the highest quantity followed by calcium and phosphate. Kaempferol and resveratrol were not determined in some nettle samples but rutin levels were determined in all samples. Vitamin A concentrations were ranged between 13.64 ± 1.90 and 5.74 ± 1.00 (mg kg(-1) dry weight). These results show that Urtica dioica L. collected from Tunceli in Turkey could be considered as a natural alternative source for food, pharmacology and medicine sectors. PMID:24199953

  19. Heart failure is associated with impaired anti-inflammatory and antioxidant properties of high-density lipoproteins.

    PubMed

    Kim, Juyong Brian; Hama, Susan; Hough, Greg; Navab, Mohamad; Fogelman, Alan M; Maclellan, W Robb; Horwich, Tamara B; Fonarow, Gregg C

    2013-12-01

    Oxidative stress and inflammation are hallmarks of the heart failure (HF) disease state. In the present study, we investigated the inflammatory/anti-inflammatory characteristics of high-density lipoproteins (HDL) in patients with HF. Ninety-six consecutive patients with systolic HF were followed in an advanced HF center, and 21 healthy subjects were recruited. Plasma was tested for HDL inflammatory index (HII) using a monocyte chemotactic activity assay, with HII >1.0 indicating proinflammatory HDL. We found significantly increased inflammatory properties of HDL in patients with HF (median HII 1.56 vs 0.59 in controls; p <0.0001). Serum amyloid A level was markedly elevated and the activity of paraoxonase-1, an HDL antioxidant enzyme, was significantly reduced in patients versus controls. HDL and albumin from patients with HF contained markedly elevated levels of oxidized products of arachidonic and linoleic acids. HDL function improved when plasma was treated in vitro with 4F, an apolipoprotein A-I mimetic peptide (40% reduction in HII, p <0.0001). There was no correlation found between HII level and ejection fraction or New York Heart Association functional class. In conclusion, HDL function is significantly impaired and oxidation products of arachidonic and linoleic acids are markedly elevated in patients with HF compared with non-HF controls. PMID:24050409

  20. Preparation of Chaga medicinal mushroom, Inonotus obliquus-fermented rice using solid-state fermentation and its taste quality and antioxidant property.

    PubMed

    Lin, Shin-Yi; Yeh, Chan-Chun; Liang, Chih-Hung; Mau, Jeng-Leun

    2012-01-01

    Chaga medicinal mushroom, Inonotus obliquus (Pers.:Fr.) Pilat, was inoculated into cooked embryo rice and the mycelial growth in I. obliquus-fermented embryo rice (IER) was monitored. Besides, nonvolatile taste components and antioxidant properties of fruiting bodies, mycelia, IER, and embryo rice were studied. The optimal conditions for mycelial growth were determined to be: 30°C, an inoculation rate of 1 mL/15 g, water supplementation of 60%, and no extra nitrogen source added. IER showed similar proximate composition to embryo rice but contained a substantial amount of ergothioneine (101 mg/kg dry weight). IER contained higher amounts of soluble sugars and polyols, and umami taste components, including monosodium glutamate (MSG)-like components and flavor 5'-nucleotides, than embryo rice. Besides, IER showed a second level of equivalent umami concentrations (223.73 g MSG/100 g). Fruiting bodies did not contain umami components but showed the most effective antioxidant properties. Although some of EC50 values of IER were less than those of mycelia and embryo rice, IER still showed effective antioxidant properties. Based on the results obtained, IER will be a novel functional food. PMID:23510252

  1. Growth, characterization and properties of ultrathin magnetic films and multilayers

    Microsoft Academic Search

    B. T. Jonker; J. P. Heremans; E. E. Marinero

    1989-01-01

    This book is a collection of invited and contributed papers presented at the Symposium on Growth, Characterization and Properties of Ultrathin Magnetic Films and Multilayers, held during the Spring Meeting of the Materials Research Society in San Diego, April 25-29, 1989. This symposium comprised 11 invited talks and 42 contributed papers, ran over 3 1\\/2 days, and provided a unique

  2. Physical property characterization of 183-H Basin sludge

    SciTech Connect

    Biyani, R.K.; Delegard, C.H.

    1995-09-20

    This document describes the characterization of 183-H Basin sludge physical properties, e.g. bulk density of sludge and absorbent, and determination of free liquids. Calcination of crucible-size samples of sludge was also done and the resulting `loss-on-ignition` was compared to the theoretical weight loss based on sludge analysis obtained from Weston Labs.

  3. Photoacoustic characterization of the mechanical properties of thin films

    Microsoft Academic Search

    Carmen M. Hernandez; Todd W. Murray; Sridhar Krishnaswamy

    Narrow band photoacoustics ~laser ultrasonics! are used to characterize the properties of free-standing nanometer-sized thin films. Photoacoustic generation is achieved by use of a microchip laser which deposits pulsed laser energy in the form of a spatially periodic source on the structure. The resulting narrow band ultrasonic modes are monitored using a Michelson interferometer. By varying the geometry of the

  4. Antioxidant and sensory properties of rice bran oil-based extraction products

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rice bran oil has many properties that make it an attractive functional food ingredient. Compared with other oils, rice bran oil is rich in tocopherols, tocotrienols, and y-oryzanol, all of which have been shown to have numerous desirable health effects. The same compounds that confer health benef...

  5. Glucose and free radicals impair the antioxidant properties of serum albumin

    Microsoft Academic Search

    EMMANUEL BOURDON; NADINE LOREAU; DENIS BLACHE

    Epidemiological data consistently show that reduced levels of serum albumin, which is the most abundant protein in plasma, are associated with an increased mortality risk. Various biological properties evidenced by direct effects of the albu- min molecule may explain its beneficial effects. The present work aimed to investigate in vitro whether glycation or free radicals or both factors would affect

  6. Genotoxic, antigenotoxic and antioxidant properties of methanol extracts obtained from Peltigera horizontalis and Peltigera praetextata.

    PubMed

    Nardemir, Gokce; Yanmis, Derya; Alpsoy, Lokman; Gulluce, Medine; Agar, Guleray; Aslan, Ali

    2015-07-01

    Now-a-days, there is a big need to reduce genotoxic effects of mutagenic and carcinogenic agents in environment, which are increased by the technological development. Lichens produce a wide variety of unique metabolites due to being in various extreme areas and being symbiotic organisms of fungi and algae. Therefore, this study was planned to search new sources having antimutagenic activity by researching two different lichen species and to determine whether their usage is safe. With this respect, the mutagenic and antimutagenic properties of methanol extracts of the lichens were determined by the bacterial reverse mutation and sister chromatid exchange assays. Furthermore, the malondialdehyde level, superoxide dismutase, glutathione and glutathione peroxidase activities against aflatoxin B1 were determined for understanding the ways in which the lichens showed their genotoxic properties. PMID:23456815

  7. The characterisation of structural and antioxidant properties of isoflavone metal chelates

    Microsoft Academic Search

    Stephen Dowling; Fiona Regan; Helen Hughes

    2010-01-01

    Isoflavone metal chelates are of interest as isoflavones act as oestrogen mimics. Metal interactions may enhance isoflavones biological properties so understanding isoflavone metal chelation is important for the commercial application of isoflavones. This work aimed to determine if isoflavones, daidzein (4',7-dihydroxyisoflavone) and genistein (4',5,7-trihydroxyisoflavone) could chelate with metals as isoflavone chelates. Biochanin A (4'-methoxy-5,7-dihydroxyisoflavone) was also examined for it's ability

  8. Antioxidant, metal-binding and DNA-damaging properties of flavonolignans: a joint experimental and computational highlight based on 7-O-galloylsilybin.

    PubMed

    Vacek, Jan; Zatloukalová, Martina; Desmier, Thomas; Nezhodová, Veronika; Hrbá?, Jan; Kubala, Martin; K?en, Vladimír; Ulrichová, Jitka; Trouillas, Patrick

    2013-10-01

    Besides the well-known chemoprotective effects of polyphenols, their prooxidant activities via interactions with biomacromolecules as DNA and proteins are of the utmost importance. Current research focuses not only on natural polyphenols but also on synthetically prepared analogs with promising biological activities. In the present study, the antioxidant and prooxidant properties of a semi-synthetic flavonolignan 7-O-galloylsilybin (7-GSB) are described. The presence of the galloyl moiety significantly enhances the antioxidant capacity of 7-GSB compared to that of silybin (SB). These findings were supported by electrochemistry, DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, total antioxidant capacity (CL-TAC) and DFT (density functional theory) calculations. A three-step oxidation mechanism of 7-GSB is proposed at pH 7.4, in which the galloyl moiety is first oxidized at Ep,1=+0.20V (vs. Ag/AgCl3M KCl) followed by oxidation of the 20-OH (Ep,2=+0.55V) and most probably 5-OH (Ep,3=+0.95V) group of SB moiety. The molecular orbital analysis and the calculation of O-H bond dissociation enthalpies (BDE) fully rationalize the electrooxidation processes. The metal (Cu(2+)) complexation of 7-GSB was studied, which appeared to involve both the galloyl moiety and the 5-OH group. The prooxidant effects of the metal-complexes were then studied according to their capacity to oxidatively induce DNA modification and cleavage. These results paved the way towards the conclusion that 7-O-galloyl substitution to SB concomitantly (i) enhances antioxidant (ROS scavenging) capacity and (ii) decreases prooxidant effect/DNA damage after Cu complexation. This multidisciplinary approach provides a comprehensive mechanistic picture of the antioxidant vs. metal-induced prooxidant effects of flavonolignans at the molecular level, under ex vivo conditions. PMID:23872255

  9. Characterization of the physical properties for solid granular materials

    SciTech Connect

    Tucker, Jonathan R. [ORISE; Shadle, Lawrence J. [U.S. DOE; Guenther, Chris [U.S. DOE; Benyahia, Sofiane [U.S. DOE; Mei, Joseph S. [U.S. DOE; Banta, Larry [WVU

    2012-01-01

    Accurate prediction of the behavior of a system is strongly governed by the components within that system. For multiphase systems incorporating solid powder-like particles, there are many different physical properties which need to be known to some level of accuracy for proper design, modeling, or data analysis. In the past, the material properties were determined initially as a secondary part of the study or design. In an attempt to provide results with the least level of uncertainty, a procedure was developed and implemented to provide consistent analysis of several different types of materials. The properties that were characterized included particle sizing and size distributions, shape analysis, density (particle, skeletal and bulk), minimum fluidization velocities, void fractions, particle porosity, and assignment within the Geldart Classification. In the methods used for this experiment, a novel form of the Ergun equation was used to determine the bulk void fractions and particle density. Materials of known properties were initially characterized to validate the accuracy and methodology, prior to testing materials of unknown properties. The procedures used yielded valid and accurate results, with a high level of repeatability. A database of these materials has been developed to assist in model validation efforts and future designs. It is also anticipated that further development of these procedures wil be expanded increasing the properties included in the database.

  10. Determination of taurine concentration during cheddar cheese manufacture and examination of antioxidant properties of whey, permeate, mother liquor, and taurine 

    E-print Network

    Li, Xin

    1999-01-01

    The objectives of this study were to determine the mass balance of taurine during the conversion of milk to cheese and during the processing of whey through lactose crystallization and to determine the antioxidant activity of whey fractions...

  11. Phenolic composition and antioxidant properties of Polish blue-berried honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization.

    PubMed

    Kusznierewicz, Barbara; Piekarska, Anna; Mrugalska, Barbara; Konieczka, Piotr; Namie?nik, Jacek; Bartoszek, Agnieszka

    2012-02-22

    In this study, different Polish cultivars of blue-berried honeysuckles (Lonicera caerulea L.), wild and bog bilberry, were analyzed for bioactive compounds. The chemical properties verified included composition of anthocyanins and other polyphenols, antioxidant activity, and profiles of antioxidants by HPLC postcolumn derivatization or TLC. The antioxidant activities of different blue-berried honeysuckle cultivars were similar to that of wild-growing bilberries (ranging from 170 to 417 ?mol TE/g dm in ABTS and from 93 to 166 ?mol TE/g dm in DPPH and Folin-Ciocalteu tests). The major anthocyanin in the blue-berried honeysuckle was cyanidin-3-glucoside, which constituted 84-92% of the total anthocyanins. The TLC and HPLC postcolumn antioxidant profiles indicated that anthocyanins are the major antioxidants in all berries studied. Wild berries and the cultivars of the blue-berried honeysuckles are also a similar source of such minerals as K, Mg, and Ca. PMID:22264130

  12. Efficacy of chemically characterized Piper betle L. essential oil against fungal and aflatoxin contamination of some edible commodities and its antioxidant activity.

    PubMed

    Prakash, Bhanu; Shukla, Ravindra; Singh, Priyanka; Kumar, Ashok; Mishra, Prashant Kumar; Dubey, Nawal Kishore

    2010-08-15

    The study investigates fungal contamination in some dry fruits, spices and areca nut and evaluation of the essential oil (EO) of Piper betle var. magahi for its antifungal, antiaflatoxigenic and antioxidant properties. A total of 1651 fungal isolates belonging to 14 species were isolated from the samples and Aspergillus was recorded as the dominant genus with 6 species. Eleven aflatoxin B(1) (AFB(1)) producing strains of A. flavus were recorded from the samples. Eugenol (63.39%) and acetyleugenol (14.05%) were the major components of 32 constituents identified from the Piper betle EO through GC and GC-MS analysis. The minimum inhibitory concentration (MIC) of P. betle EO was found 0.7 microl/ml against A.flavus. The EO reduced AFB(1) production in a dose dependent manner and completely inhibited at 0.6 microl/ml. This is the first report on efficacy of P. betle EO as aflatoxin suppressor. EO also exhibited strong antioxidant potential as its IC(50) value (3.6 microg/ml) was close to that of ascorbic acid (3.2 microg/ml) and lower than that of the synthetic antioxidants such as butylated hydroxytouene (BHT) (7.4 microg/ml) and butylated hydroxyanisole (BHA) (4.5 microg/ml). P. betle EO thus exhibited special merits possessing antifungal, aflatoxin suppressive and antioxidant characters which are desirable for an ideal preservative. Hence, its application as a plant based food additive in protection and enhancement of shelf life of edible commodities during storage and processing is strongly recommended in view of the toxicological implications by synthetic preservatives. PMID:20621374

  13. Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage ( Borago officinalis L.) seeds

    Microsoft Academic Search

    Mahinda Wettasinghe; Fereidoon Shahidi

    1999-01-01

    Borage meal exerted a concentration-dependent antioxidant activity in a meat model system. At 2% (w\\/w), it inhibited (p?0.05) 2-thiobarbituric acid-reactive substances (TBARS), hexanal and total volatile formation in meat by 26.5, 30.5 and 18.6%, respectively. Antioxidant compounds in the meal were concentrated at optimum extraction conditions (in 52% ethanol at 74°C for 62 min) predicted by response surface methodology (RSM).

  14. Acetylcholinesterase inhibitory and antioxidant properties of Cyclotrichium niveum, Thymus praecox subsp. caucasicus var. caucasicus, Echinacea purpurea and E. pallida

    Microsoft Academic Search

    I. Orhan; F. S. ?enol; A. R. Gülpinar; M. Kartal; N. ?ekeroglu; M. Deveci; Y. Kan; B. ?ener

    2009-01-01

    The dichloromethane, ethyl acetate, ethanol, and aqueous extracts of Cyclotrichium niveum (CN) and Thymus praecox subsp. caucasicus var. caucasicus (TP), Echinacea purpurea (EPU), and E. pallida (EPA) along with the essential oils of CN and TP were assessed for their anti-acetylcholinesterase (AChE) and antioxidant activities. AChE inhibition was estimated using spectrophotometric method of Ellman. Antioxidant activity was evaluated by 2,2-diphenyl-2-picrylhydrazyl

  15. Assessing the bioavailability of polyphenols and antioxidant properties of extra virgin argan oil by simulated digestion and Caco-2 cell assays. Comparative study with extra virgin olive oil.

    PubMed

    Seiquer, Isabel; Rueda, Ascensión; Olalla, Manuel; Cabrera-Vique, Carmen

    2015-12-01

    Argan oil is becoming increasingly popular in the edible-oil market as a luxury food with healthy properties. This paper analyzes (i) the bioavailability of the polyphenol content and antioxidant properties of extra virgin argan oil (EVA) by the combination of in vitro digestion and absorption across Caco-2 cells and (ii) the protective role of the oil bioaccessible fraction (BF) against induced oxidative stress. Results were compared with those obtained with extra virgin olive oil (EVO). Higher values of polyphenols and antioxidant activity were observed in the BF obtained after the in vitro digestion of oils compared with the initial chemical extracts; the increase was higher for EVA but absolute BF values were lower than EVO. Bioaccessible polyphenols from EVA were absorbed by Caco-2 cells in higher proportions than from EVO, and minor differences were observed for antioxidant activity. Preincubation of cell cultures with BF from both oils significantly protected against oxidation, limiting cell damage and reducing reactive oxygen species generation. PMID:26041223

  16. Antioxidant properties, phenolic composition and potentiometric sensor array evaluation of commercial and new blueberry (Vaccinium corymbosum) and bog blueberry (Vaccinium uliginosum) genotypes.

    PubMed

    Kraujalyt?, Vilma; Venskutonis, Petras Rimantas; Pukalskas, Audrius; ?esonien?, Laima; Daubaras, Remigijus

    2015-12-01

    Antioxidant properties of juices of newly bred and known blueberry (Vaccinium corymbosum) genotypes and wild bog blueberry (Vaccinium uliginosum) were evaluated by ABTS(+) scavenging capacity (RSC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), total phenolic content (TPC) and total anthocyanin content (TAC) assays. TPC varied in the range of 0.85-2.81mg gallic acid equiv./mL, RSC, FRAP and ORAC values were 6.38-20.9, 3.07-17.8 and 4.21-45.68?mol Trolox equiv./g, respectively. New blueberry genotypes and bog blueberry demonstrated stronger antioxidant properties and TAC than other studied genotypes. The content of quinic (203-3614?g/mL), chlorogenic (20.0-346.8?g/mL) acids and rutin (0.00-26.88?g/mL) measured by UPLC/ESI-QTOF-MS varied depending on the genotype. Juices were evaluated by electronic tongue; PCA score plot showed that the method discriminates different genotypes although some juice samples were located very closely and overlapping. Significant differences were observed between L(?), a(?), b(?) colour parameters of some genotypes. PMID:26041234

  17. Augmentation of water-holding and textural properties of breast meat from oxidatively stressed broilers by dietary antioxidant regimens.

    PubMed

    Delles, R M; Xiong, Y L; True, A D; Ao, T; Dawson, K A

    2015-06-01

    1. The impact of dietary antioxidants and degree of oil oxidation on textural attributes of chicken broiler breast meat stored in oxygen-enriched, air-permeable polyvinylchloride and skin packaging systems during retail display at 2-4°C for up to 21 d was assessed. 2. Broilers were fed on diets either with a low-oxidised oil (peroxide 23 mEq O2/kg) or with a high-oxidised oil (peroxide 121 mEq O2/kg), with or without an algae-based antioxidant and organic mineral antioxidant supplement for 42 d. 3. Fatty acids and radical scavenging activities of the diets were estimated. Meat colour, pH, myofibrillar protein profile and textural traits were measured. 4. Diets with high-oxidised oil reduced stearic, linoleic and linolenic acid content compared to low-oxidised oil samples, regardless of antioxidant supplementation. Meat colour and pH varied among dietary treatments throughout storage. Meat samples from the antioxidant dietary group, irrespective of oil oxidation level, had lower amounts of purge and cooking losses compared to the unsupplemented diets. For all packaging systems, meat shear force was significantly higher for broilers fed on high-oxidised diets. 5. The results demonstrate that dietary antioxidant supplementation can minimise the negative impact of oxidised oil on the quality of broiler meat packaged in different atmospheric environments. PMID:25854630

  18. Isolation of quercetin's salts and studies of their physicochemical properties and antioxidant relationships.

    PubMed

    Milane, Hadi A; Ubeaud, Geneviève; Vandamme, Thierry F; Jung, Louis

    2004-07-01

    Hydroxyflavones in alkaline solutions show high free radical scavenging activities. Quercetin, one of these hydroxyflavones may be submitted to chemical reactions yielding a mixture of mono-, di- and tri-sodium salts. These salts were recovered after solubilization and stepwise precipitation in methylalcohol/ethylacetate solvents. The different salts were analyzed using sodium emission spectrophotometry and nuclear magnetic resonance to determine the number of acid hydrogens at pH10 and the position of these acid hydrogens. Our study demonstrates that among the three salts of quercetin, the di-sodium compound is endowed with the more efficient scavenging properties in a phosphate buffer at physiological pH7.4. Physicochemical parameters and free hydroxyl radical scavenging activity relationships were also determined, allowing to explain the mechanisms whereby hydroxyl groups exert their radical scavenging activities. PMID:15186847

  19. Allium schoenoprasum L., as a natural antioxidant.

    PubMed

    Stajner, D; Canadanovi?-Brunet, J; Pavlovi?, A

    2004-07-01

    The present study investigated the antioxidative properties of the bulb, leaf and stalk of Allium schoenoprasum L. Activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, glutathione peroxidase), quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and also the content of total flavonoids, chlorophylls a and b, carotenoids, vitamin C and soluble proteins were determined. The results indicate that extracts from all plant organs exhibited antioxidant activity. The highest antioxidant activity was observed in the leaves. PMID:15305309

  20. Assessment of In vivo antioxidant properties of Dacryodes edulis and Ficus exasperata as anti-malaria plants

    PubMed Central

    Conrad, Omonhinmin A; Uche, Agbara I

    2013-01-01

    Objective To evaluate the phytochemical profile and potential anti-oxidant properties of Dacryodes edulis and Ficus exasperata. Methods Six groups of Albino rats were intoxicated with tetrachloromethane (CCl4) for 2 d, prior to 7 d administration of 50 mg/kg, 100 mg/kg, 200 mg/kg ethanol extracts of plants and three control remidies which is Tween 80 (placebo), CCl4 (negative control) and vitamin E (positive control). Tissue homogenates were employed in assessing the thiobarbituric acid reactive substances expressed as malondialdehyde (MDA) levels, reduced glutathione (GSH) and catalase (CAT) activities. Results Phytochemical profiling of the plants showed the presence of reducing sugars, flavonoids, saponins and tannins, except alkaloids and terpenoids in F. exasperata and cardiac glycosides in D. edulis. Generally, significantly different values (P<0.05) were recorded for blood than for liver homogenates. Elevated MDA levels were observed for the CCl4 treated group (negative control). However, lower MDA levels comparable to vitamin E (positive control) were recorded for all D. edulis and the 200 mg/kg F. exasperata pre-treatments. CAT levels were significantly (P<0.05) raised in the 100 mg/kg and 200 mg/kg pretreatments for blood and the 200 mg/kg pretreatments for liver, than for Vit E. CCl4 reduced GSH levels were reversed significantly (P<0.05) in blood by D. edulis and by the 100 mg/kg and 200 mg/kg F. exasperata pretreatments in blood and liver tissues. The mean dose-dependent analysis shows increasing fall in MDA levels with dosage. Conclusions The plant extracts exhibited dose-dependent oxidative stress suppressive action. This may justify their use for the traditional preparation of anti-malarial remedies.