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Sample records for characterization experiment ace-2

  1. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.

    PubMed

    Ge, Xing-Yi; Li, Jia-Lu; Yang, Xing-Lou; Chmura, Aleksei A; Zhu, Guangjian; Epstein, Jonathan H; Mazet, Jonna K; Hu, Ben; Zhang, Wei; Peng, Cheng; Zhang, Yu-Ji; Luo, Chu-Ming; Tan, Bing; Wang, Ning; Zhu, Yan; Crameri, Gary; Zhang, Shu-Yi; Wang, Lin-Fa; Daszak, Peter; Shi, Zheng-Li

    2013-11-28

    The 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV) was one of the most significant public health events in recent history. An ongoing outbreak of Middle East respiratory syndrome coronavirus suggests that this group of viruses remains a key threat and that their distribution is wider than previously recognized. Although bats have been suggested to be the natural reservoirs of both viruses, attempts to isolate the progenitor virus of SARS-CoV from bats have been unsuccessful. Diverse SARS-like coronaviruses (SL-CoVs) have now been reported from bats in China, Europe and Africa, but none is considered a direct progenitor of SARS-CoV because of their phylogenetic disparity from this virus and the inability of their spike proteins to use the SARS-CoV cellular receptor molecule, the human angiotensin converting enzyme II (ACE2). Here we report whole-genome sequences of two novel bat coronaviruses from Chinese horseshoe bats (family: Rhinolophidae) in Yunnan, China: RsSHC014 and Rs3367. These viruses are far more closely related to SARS-CoV than any previously identified bat coronaviruses, particularly in the receptor binding domain of the spike protein. Most importantly, we report the first recorded isolation of a live SL-CoV (bat SL-CoV-WIV1) from bat faecal samples in Vero E6 cells, which has typical coronavirus morphology, 99.9% sequence identity to Rs3367 and uses ACE2 from humans, civets and Chinese horseshoe bats for cell entry. Preliminary in vitro testing indicates that WIV1 also has a broad species tropism. Our results provide the strongest evidence to date that Chinese horseshoe bats are natural reservoirs of SARS-CoV, and that intermediate hosts may not be necessary for direct human infection by some bat SL-CoVs. They also highlight the importance of pathogen-discovery programs targeting high-risk wildlife groups in emerging disease hotspots as a strategy for pandemic preparedness. PMID:24172901

  2. Clear Sky Column Closure Studies of Urban-Marine and Mineral-Dust Aerosols Using Aircraft, Ship, Satellite and Ground-Based Measurements in ACE-2

    NASA Technical Reports Server (NTRS)

    Schmid, Beat; Russell, Philip B.; Livingston, John M.; Gasso, Santiago; Hegg, Dean A.; Collins, Donald R.; Flagan, Richard C.; Seinfeld, John H.; Oestroem, Elisabeth; Noone, Kevin J.; Durkee, Philip A.; Jonsson, Haflidi H.; Welton, Ellsworth J.; Voss, Kenneth J.; Gordon, Howard R.; Formenti, Paola; Andreae, Meinrat O.; Kapustin, Vladimir N.; Bates, Timothy S.; Quinn, Patricia K.

    2000-01-01

    As part of the second Aerosol Characterization Experiment (ACE-2), European urban-marine and African mineral-dust aerosols were measured aboard the Pelican aircraft, the Research Vessel Vodyanitskiy from the ground and from satellites.

  3. Defective intestinal amino acid absorption in Ace2 null mice.

    PubMed

    Singer, Dustin; Camargo, Simone M R; Ramadan, Tamara; Schäfer, Matthias; Mariotta, Luca; Herzog, Brigitte; Huggel, Katja; Wolfer, David; Werner, Sabine; Penninger, Josef M; Verrey, François

    2012-09-15

    Mutations in the main intestinal and kidney luminal neutral amino acid transporter B(0)AT1 (Slc6a19) lead to Hartnup disorder, a condition that is characterized by neutral aminoaciduria and in some cases pellagra-like symptoms. These latter symptoms caused by low-niacin are thought to result from defective intestinal absorption of its precursor L-tryptophan. Since Ace2 is necessary for intestinal B(0)AT1 expression, we tested the impact of intestinal B(0)AT1 absence in ace2 null mice. Their weight gain following weaning was decreased, and Na(+)-dependent uptake of B(0)AT1 substrates measured in everted intestinal rings was defective. Additionally, high-affinity Na(+)-dependent transport of L-proline, presumably via SIT1 (Slc6a20), was absent, whereas glucose uptake via SGLT1 (Slc5a1) was not affected. Measurements of small intestine luminal amino acid content following gavage showed that more L-tryptophan than other B(0)AT1 substrates reach the ileum in wild-type mice, which is in line with its known lower apparent affinity. In ace2 null mice, the absorption defect was confirmed by a severalfold increase of L-tryptophan and of other neutral amino acids reaching the ileum lumen. Furthermore, plasma and muscle levels of glycine and L-tryptophan were significantly decreased in ace2 null mice, with other neutral amino acids displaying a similar trend. A low-protein/low-niacin diet challenge led to differential changes in plasma amino acid levels in both wild-type and ace2 null mice, but only in ace2 null mice to a stop in weight gain. Despite the combination of low-niacin with a low-protein diet, plasma niacin concentrations remained normal in ace2 null mice and no pellagra symptoms, such as photosensitive skin rash or ataxia, were observed. In summary, mice lacking Ace2-dependent intestinal amino acid transport display no total niacin deficiency nor clear pellagra symptoms, even under a low-protein and low-niacin diet, despite gross amino acid homeostasis alterations

  4. North Atlantic Aerosol Properties for Radiative Impact Assessments. Derived from Column Closure Analyses in TARFOX and ACE-2

    NASA Technical Reports Server (NTRS)

    Russell, Philip A.; Bergstrom, Robert A.; Schmid, Beat; Livingston, John M.

    2000-01-01

    Aerosol effects on atmospheric radiative fluxes provide a forcing function that can change the climate in potentially significant ways. This aerosol radiative forcing is a major source of uncertainty in understanding the climate change of the past century and predicting future climate. To help reduce this uncertainty, the 1996 Tropospheric Aerosol Radiative Forcing Observational Experiment (TARFOX) and the 1997 Aerosol Characterization Experiment (ACE-2) measured the properties and radiative effects of aerosols over the Atlantic Ocean. Both experiments used remote and in situ measurements from aircraft and the surface, coordinated with overpasses by a variety of satellite radiometers. TARFOX focused on the urban-industrial haze plume flowing from the United States over the western Atlantic, whereas ACE-2 studied aerosols over the eastern Atlantic from both Europe and Africa. These aerosols often have a marked impact on satellite-measured radiances. However, accurate derivation of flux changes, or radiative forcing, from the satellite measured radiances or retrieved aerosol optical depths (AODs) remains a difficult challenge. Here we summarize key initial results from TARFOX and ACE-2, with a focus on closure analyses that yield aerosol microphysical models for use in improved assessments of flux changes. We show how one such model gives computed radiative flux sensitivities (dF/dAOD) that agree with values measured in TARFOX and preliminary values computed for the polluted marine boundary layer in ACE-2. A companion paper uses the model to compute aerosol-induced flux changes over the North Atlantic from AVHRR-derived AOD fields.

  5. North Atlantic Aerosol Properties and Direct Radiative Effects: Key Results from TARFOX and ACE-2

    NASA Technical Reports Server (NTRS)

    Russell, P. B.; Livingston, J. M.; Schmid, B.; Bergstrom, R. A.; Hignett, P.; Hobbs, P. V.; Durkee, P. A.; Condon, Estelle (Technical Monitor)

    1998-01-01

    Aerosol effects on atmospheric radiative fluxes provide a forcing function that can change the climate in potentially significant ways. This aerosol radiative Forcing is a major source of uncertainty in understanding the observed climate change of the past century and in predicting, future climate. To help reduce this uncertainty, the International Global Atmospheric Chemistry Project (IGAC) has endorsed a series of multiplatform aerosol field campaigns. The Tropospheric Aerosol Radiative Forcing Observational Experiment (TARFOX) and the second Aerosol Characterization Experiment (ACE-2) were the first IGAC campaigns to address the impact of anthropogenic aerosols. Both TARFOX and ACE-2 gathered extensive data sets on aerosol properties and radiative effects. TARFOX focused on the urban-industrial haze plume flowing from the eastern United States over the western Atlantic Ocean, whereas ACE-2 studied aerosols carried over the eastern Atlantic from both European urban/industrial and African mineral sources. These aerosols often have a marked influence on the top-of-atmosphere radiances measured by satellites, as illustrated in Figure 1. Shown there are contours of aerosol optical depth derived from radiances measured by the AVHRR sensor on the NOAA-11 satellite. The contours readily show that aerosols originating in North America, Europe, and Africa impact the radiative properties of air over the North Atlantic. However, the accurate derivation of flux chances, or radiative forcing, from the satellite-measured radiances or 'etrieved optical depths remains a difficult challenge. In this paper we summarize key Initial results from TARFOX and, to a lesser extent ACE-2, with a focus on those results that allow an improved assessment of the flux changes caused by North Atlantic aerosols at middle and high latitudes.

  6. North Atlantic Aerosol Properties and Direct Radiative Effects: Key Results from TARFOX and ACE-2

    NASA Technical Reports Server (NTRS)

    Russell, P. B.; Livingston, J. M.; Schmid, B.; Bergstrom, Robert A.; Hignett, P.; Hobbs, P. V.; Durkee, P. A.

    2000-01-01

    Aerosol effects on atmospheric radiative fluxes provide a forcing function that can change the climate In potentially significant ways. This aerosol radiative forcing is a major source of uncertainty in understanding the observed climate change of the past century and in predicting future climate. To help reduce this uncertainty, the International Global Atmospheric Chemistry Project (IGAC) has endorsed a series of multiplatform aerosol field campaigns. The Tropospheric Aerosol Radiative Forcing Observational Experiment (TARFOX) and the second Aerosol Characterization Experiment (ACE-2) were the first IGAC campaigns to address the impact of anthropogenic aerosols, Both TARFOX and ACE-2 gathered extensive data sets on aerosol properties and radiative effects, TARFOX focused on the urban-industrial haze plume flowing from the eastern United States over the western Atlantic Ocean, whereas ACE-2 studied aerosols carried over the eastern Atlantic from both European urban/industrial and African mineral sources. These aerosols often have a marked influence on the top-of-atmosphere radiances measured by satellites. Shown there are contours of aerosol optical depth derived from radiances measured by the AVHRR sensor on the NOAA-11 satellite. The contours readily show that aerosols originating in North America, Europe, and Africa impact the radiative properties of air over the North Atlantic. However, the accurate derivation of flux changes, or radiative forcing, from the satellite measured radiances or retrieved optical depths remains a difficult challenge. In this paper we summarize key initial results from TARFOX and, to a lesser extent, ACE-2, with a focus on those results that allow an improved assessment of the flux changes caused by North Atlantic aerosols at middle latitudes.

  7. Human Recombinant ACE2 Reduces the Progression of Diabetic Nephropathy

    PubMed Central

    Oudit, Gavin Y.; Liu, George C.; Zhong, JiuChang; Basu, Ratnadeep; Chow, Fung L.; Zhou, Joyce; Loibner, Hans; Janzek, Evelyne; Schuster, Manfred; Penninger, Josef M.; Herzenberg, Andrew M.; Kassiri, Zamaneh; Scholey, James W.

    2010-01-01

    OBJECTIVE Diabetic nephropathy is one of the most common causes of end-stage renal failure. Inhibition of ACE2 function accelerates diabetic kidney injury, whereas renal ACE2 is downregulated in diabetic nephropathy. We examined the ability of human recombinant ACE2 (hrACE2) to slow the progression of diabetic kidney injury. RESEARCH DESIGN AND METHODS Male 12-week-old diabetic Akita mice (Ins2WT/C96Y) and control C57BL/6J mice (Ins2WT/WT) were injected daily with placebo or with rhACE2 (2 mg/kg, i.p.) for 4 weeks. Albumin excretion, gene expression, histomorphometry, NADPH oxidase activity, and peptide levels were examined. The effect of hrACE2 on high glucose and angiotensin II (ANG II)–induced changes was also examined in cultured mesangial cells. RESULTS Treatment with hrACE2 increased plasma ACE2 activity, normalized blood pressure, and reduced the urinary albumin excretion in Akita Ins2WT/C96Y mice in association with a decreased glomerular mesangial matrix expansion and normalization of increased α-smooth muscle actin and collagen III expression. Human recombinant ACE2 increased ANG 1–7 levels, lowered ANG II levels, and reduced NADPH oxidase activity. mRNA levels for p47phox and NOX2 and protein levels for protein kinase Cα (PKCα) and PKCβ1 were also normalized by treatment with hrACE2. In vitro, hrACE2 attenuated both high glucose and ANG II–induced oxidative stress and NADPH oxidase activity. CONCLUSIONS Treatment with hrACE2 attenuates diabetic kidney injury in the Akita mouse in association with a reduction in blood pressure and a decrease in NADPH oxidase activity. In vitro studies show that the protective effect of hrACE2 is due to reduction in ANG II and an increase in ANG 1–7 signaling. PMID:19934006

  8. Genetic Deletion of ACE2 Induces Vascular Dysfunction in C57BL/6 Mice: Role of Nitric Oxide Imbalance and Oxidative Stress

    PubMed Central

    Rabelo, Luiza A.; Todiras, Mihail; Nunes-Souza, Valéria; Qadri, Fatimunnisa; Szijártó, István András; Gollasch, Maik; Penninger, Josef M.; Bader, Michael; Santos, Robson A.; Alenina, Natalia

    2016-01-01

    Accumulating evidence indicates that angiotensin-converting enzyme 2 (ACE2) plays a critical role in cardiovascular homeostasis, and its altered expression is associated with major cardiac and vascular disorders. The aim of this study was to evaluate the regulation of vascular function and assess the vascular redox balance in ACE2-deficient (ACE2-/y) animals. Experiments were performed in 20–22 week-old C57BL/6 and ACE2-/y male mice. Evaluation of endothelium-dependent and -independent relaxation revealed an impairment of in vitro and in vivo vascular function in ACE2-/y mice. Drastic reduction in eNOS expression at both protein and mRNA levels, and a decrease in •NO concentrations were observed in aortas of ACE2-/y mice in comparison to controls. Consistently, these mice presented a lower plasma and urine nitrite concentration, confirming reduced •NO availability in ACE2-deficient animals. Lipid peroxidation was significantly increased and superoxide dismutase activity was decreased in aorta homogenates of ACE2-/y mice, indicating impaired antioxidant capacity. Taken together, our data indicate, that ACE2 regulates vascular function by modulating nitric oxide release and oxidative stress. In conclusion, we elucidate mechanisms by which ACE2 is involved in the maintenance of vascular homeostasis. Furthermore, these findings provide insights into the role of the renin-angiotensin system in both vascular and systemic redox balance. PMID:27070147

  9. Radiative Flux Changes by Aerosols from North America, Europe, and Africa over the Atlantic Ocean: Measurements and Calculations from TARFOX and ACE-2

    NASA Technical Reports Server (NTRS)

    Russell, P. B.; Hignett, P.; Livingston, J. M.; Schmid, B.; Chien, A.; Bergstrom, R.; Durkee, P. A.; Hobbs, P. V.; Bates, T. S.; Quinn, P. K.; Condon, Estelle (Technical Monitor)

    1998-01-01

    Aerosol effects on atmospheric radiative fluxes provide a forcing function that is a major source of uncertainty in understanding the past climate and predicting climate change. To help reduce this uncertainty, the 1996 Tropospheric Aerosol Radiative Forcing Experiment (TARFOX) and the 1997 second Aerosol Characterization Experiment (ACE-2) measured the properties and radiative effects of American, European, and African aerosols over the Atlantic. In TARFOX, radiative fluxes and microphysics of the American aerosol were measured from the UK C-130 while optical depth spectra, aerosol composition, and other properties were measured by the University of Washington C-131A and the CIRPAS Pelican. Closure studies show that the measured flux changes agree with those derived from the aerosol measurements using several modelling approaches. The best-fit midvisible single-scatter albedos (approx. 0.89 to 0.93) obtained from the TARFOX flux comparisons are in accord with values derived by independent techniques. In ACE-2 we measured optical depth and extinction spectra for both European urban-marine aerosols and free-tropospheric African dust aerosols, using sunphotometers on the R/V Vodyanitskiy and the Pelican. Preliminary values for the radiative flux sensitivities (Delta Flux / Delta Optical depth) computed for ACE-2 aerosols (boundary layer and African dust) over ocean are similar to those found in TARFOX. Combining a satellite-derived optical depth climatology with the aerosol optical model validated for flux sensitivities in TARFOX provides first-cut estimates of aerosol-induced flux changes over the Atlantic Ocean.

  10. ACE-2 HILLCLOUD: AN OVERVIEW OF THE ACE-2 GROUND BASED CLOUD EXPERIMENT. (U915364)

    EPA Science Inventory

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  11. ACE2/Ang 1-7 axis: A critical regulator of epicardial adipose tissue inflammation and cardiac dysfunction in obesity.

    PubMed

    Patel, Vaibhav B; Basu, Ratnadeep; Oudit, Gavin Y

    2016-01-01

    Obesity is characterized by an excessive fat accumulation in adipose tissues leading to weight gain and is increasing in prevalence and is strongly associated with metabolic and cardiovascular disorders. The renin-angiotensin system (RAS) has emerged as a key pathogenic mechanism for these disorders; activated RAS and angiotensin (Ang) II production results in worsening of cardiovascular diseases and angiotensin converting enzyme 2 (ACE2) negatively regulates RAS by metabolizing Ang II into Ang 1-7. ACE2 is expressed in the adipocytes and its expression is upregulated in response to high fat diet induced obesity in mice. Loss of ACE2 results in heart failure with preserved ejection fraction which is mediated in part by epicardial adipose tissue inflammation. Angiotensin 1-7 reduces the obesity associated cardiac dysfunction predominantly via its role in adiponectin expression and attenuation of epicardial adipose tissue inflammation. Human heart disease is also linked with inflammed epicardial adipose tissue. Here, we discuss the important interpretation of the novel of ACE2/Ang 1-7 pathway in obesity associated cardiac dysfunction. PMID:27617176

  12. ACE2 Decreases Formation and Severity of Angiotensin II-induced Abdominal Aortic Aneurysms

    PubMed Central

    Thatcher, Sean E.; Zhang, Xuan; Howatt, Deborah A.; Yiannikouris, Frederique; Gurley, Susan B.; Ennis, Terri; Curci, John A.; Daugherty, Alan; Cassis, Lisa A.

    2014-01-01

    Objective Angiotensin converting enzyme 2 (ACE2) cleaves angiotensin II (AngII) to form angiotensin-(1-7) (Ang-(1-7)), which generally opposes effects of AngII. AngII infusion into hypercholesterolemic male mice induces formation of abdominal aortic aneurysms (AAAs). This study tests the hypothesis that deficiency of ACE2 promotes AngII-induced AAAs, while ACE2 activation suppresses aneurysm formation. Approach and Results ACE2 protein was detectable by immunostaining in mice and human AAAs. Whole body deficiency of ACE2 significantly increased aortic lumen diameters and external diameters of suprarenal aortas from AngII-infused mice. Conversely, ACE2 deficiency in bone marrow-derived cells had no effect on AngII-induced AAAs. In contrast to AngII-induced AAAs, ACE2 deficiency had no significant effect on external aortic diameters of elastase-induced AAAs. Since ACE2 deficiency promoted AAA formation in AngII-infused mice, we determined if ACE2 activation suppressed AAAs. ACE2 activation by administration of diminazine aceturate (DIZE, 30 mg/kg/day) to Ldlr−/− mice increased kidney ACE2 mRNA abundance and activity and elevated plasma Ang-(1-7) concentrations. Unexpectedly, administration of DIZE significantly reduced total sera cholesterol and VLDL-cholesterol concentrations. Notably, DIZE significantly decreased aortic lumen diameters and aortic external diameters of AngII-infused mice resulting in a marked reduction in AAA incidence (from 73 to 29%). None of these effects of DIZE were observed in the Ace2−/y mice. Conclusions These results demonstrate that ACE2 exerts a modulatory role in AngII-induced AAA formation, and that therapeutic stimulation of ACE2 could be a benefit to reduce AAA expansion and rupture in patients with an activated renin-angiotensin system. PMID:25301841

  13. Ground-Based Lidar Measurements of Aerosols During ACE-2 Instrument Description, Results, and Comparisons with Other Ground-Based and Airborne Measurements

    NASA Technical Reports Server (NTRS)

    Welton, Ellsworth J.; Voss, Kenneth J.; Gordon, Howard R.; Maring, Hal; Smirnov, Alexander; Holben, Brent; Schmid, Beat; Livingston, John M.; Russell, Philip B.; Durkee, Philip A.; Formenti, Paolo

    2000-01-01

    A micro-pulse lidar system (MPL) was used to measure the vertical and horizontal distribution or aerosols during the Aerosol Characterization Experiment 2 (ACE-2) in June and July of 1997. The MPL measurements were made at the Izana observatory (IZO), a weather station located on a mountain ridge (28 deg 18'N, 16 deg 30'W, 2367 m asl) near the center of the island of Tenerife, Canary Islands. The MPL was used to acquire aerosol backscatter, extinction, and optical depth profiles for normal background periods and periods influenced by Saharan dust from North Africa. System tests and calibration procedures are discussed, and in analysis of aerosol optical profiles acquired during ACE-2 is presented. MPL data taken during normal IZO conditions (no dust) showed that upslope aerosols appeared during the day and dissipated at night and that the layers were mostly confined to altitudes a few hundred meters above IZO. MPL data taken during a Saharan dust episode on 17 July showed that peak aerosol extinction values were an order of magnitude greater than molecular scattering over IZO. and that the dust layers extended to 5 km asl. The value of the dust backscatter-extinction ratio was determined to be 0.027 + 0.007 per sr. Comparisons of the MPL data with data from other co-located instruments showed good agreement during the dust episode.

  14. Ground-Based Lidar Measurements of Aerosols During ACE-2: Instrument Description, Results, and Comparisons with Other Ground-Based and Airborne Measurements

    NASA Technical Reports Server (NTRS)

    Welton, Ellsworth J.; Voss, Kenneth J.; Gordon, Howard R.; Maring, Hal; Smirnov, Alexander; Holben, Brent; Schmid, Beat; Livingston, John M.; Russell, Philip B.; Durkee, Philip A.

    2000-01-01

    A micro-pulse lidar system (MPL) was used to measure the vertical and horizontal distribution of aerosols during the Aerosol Characterization Experiment 2 (ACE-2) in June and July of 1997. The MPL measurements were made at the Izana observatory (IZO), a weather station located on a mountain ridge (28 deg 18 min N, 16 deg 30 min W, 2367 m asl) near the center of the island of Tenerife, Canary Islands. The MPL was used to acquire aerosol backscatter, extinction, and optical depth profiles for normal background periods and periods influenced by Saharan dust from North Africa. System tests and calibration procedures are discussed, and an analysis of aerosol optical profiles acquired during ACE-2 is presented. MPL data taken during normal IZO conditions (no dust) showed that upslope aerosols appeared during the day and dissipated at night and that the layers were mostly confined to altitudes a few hundred meters above IZO. MPL data taken during a Saharan dust episode on 17 July showed that peak aerosol extinction values were an order of magnitude greater than molecular scattering over IZO, and that the dust layers extended to 5 km asl. The value of the dust backscatter-extinction ratio was determined to be 0.027 +/- 0.007 sr(exp -1). Comparisons of the MPL data with data from other collocated instruments showed good agreement during the dust episode.

  15. ACE2 Global Digital Elevation Model : User Analysis

    NASA Astrophysics Data System (ADS)

    Smith, R. G.; Berry, P. A. M.; Benveniste, J.

    2013-12-01

    Altimeter Corrected Elevations 2 (ACE2), first released in October 2009, is the Global Digital Elevation Model (GDEM) created by fusing the high accuracy of over 100 million altimeter retracked height estimates, derived primarily from the ERS-1 Geodetic Mission, with the high frequency content available within the near-global Shuttle Radar Topography Mission. This novel ACE2 GDEM is freely available at 3”, 9”, 30” and 5' and has been distributed via the web to over 680 subscribers. This paper presents the results of a detailed analysis of geographical distribution of subscribed users, along with fields of study and potential uses. Investigations have also been performed to determine the most popular spatial resolutions and the impact these have on the scope of data downloaded. The analysis has shown that, even though the majority of users have come from Europe and America, a significant number of website hits have been received from South America, Africa and Asia. Registered users also vary widely, from research institutions and major companies down to individual hobbyists looking at data for single projects.

  16. The ACE2/Ang-(1-7)/Mas Axis Regulates the Development of Pancreatic Endocrine Cells in Mouse Embryos

    PubMed Central

    Wang, Lin; Liang, Juan; Leung, Po Sing

    2015-01-01

    Angiotensin-converting enzyme 2 (ACE2), its product Angiotensin-(1-7) [Ang-(1-7)], and Ang-(1-7) receptor Mas, have been shown to regulate organogenesis during embryonic development in various species. However, it is not known whether a local ACE2/Ang-(1-7)/Mas axis is present in the fetal pancreas. It is hypothesized that there is a local ACE2/Ang-(1-7)/Mas axis in the embryonic pancreas in mice that is involved in regulating islet cell development. To address this issue, the endogenous expression profile of axis constituents in embryonic mouse pancreata was examined. Involvement of the ACE2 axis in the regulation of pancreatic development was also examined. The present experiments showed in an in vivo animal model that endogenous expression levels of ACE2 and the Mas receptor were upregulated in mouse pancreata in late embryogenesis, peaking on embryonic day E16.5, when it reached 3 folds compared to that seen at E12.5. Consistently, endogenous expression of Ang-(1-7) also peaked at E16.5. Treatment with the ACE2 inhibitor DX600 did not alter islet development. However, prenatal treatment with A779, a Mas receptor antagonist, reduced the β-cell to α-cell ratio in neonatal islets, impaired islet insulin secretory function, and impaired the pups’ glucose tolerance. In ex vivo pancreas explant cultures, A779 again decreased the β-cell to α-cell ratio, apparently through its effects on β-cell proliferation (reduced proliferation shown with Ki67 staining), and also decreased Insulin and Ngn3 mRNA expression. Furthermore, treatment of explant cultures with Ang-(1-7) increased mRNA levels of Insulin and pancreatic progenitor marker Ngn3, as well as Nox4, the ROS generation enzyme; these stimulatory effects were attenuated by co-treatment with A779, suggesting that Ang-(1-7), via Mas receptor signaling, may promote differentiation of pancreatic progenitors into insulin-producing cells via modulation of reactive oxygen species. These data together suggest that a

  17. From gene to protein—experimental and clinical studies of ACE2 in blood pressure control and arterial hypertension

    PubMed Central

    Patel, Sheila K.; Velkoska, Elena; Freeman, Melanie; Wai, Bryan; Lancefield, Terase F.; Burrell, Louise M.

    2014-01-01

    Hypertension is a major risk factor for stroke, coronary events, heart and renal failure, and the renin-angiotensin system (RAS) plays a major role in its pathogenesis. Within the RAS, angiotensin converting enzyme (ACE) converts angiotensin (Ang) I into the vasoconstrictor Ang II. An “alternate” arm of the RAS now exists in which ACE2 counterbalances the effects of the classic RAS through degradation of Ang II, and generation of the vasodilator Ang 1-7. ACE2 is highly expressed in the heart, blood vessels, and kidney. The catalytically active ectodomain of ACE2 undergoes shedding, resulting in ACE2 in the circulation. The ACE2 gene maps to a quantitative trait locus on the X chromosome in three strains of genetically hypertensive rats, suggesting that ACE2 may be a candidate gene for hypertension. It is hypothesized that disruption of tissue ACE/ACE2 balance results in changes in blood pressure, with increased ACE2 expression protecting against increased blood pressure, and ACE2 deficiency contributing to hypertension. Experimental hypertension studies have measured ACE2 in either the heart or kidney and/or plasma, and have reported that deletion or inhibition of ACE2 leads to hypertension, whilst enhancing ACE2 protects against the development of hypertension, hence increasing ACE2 may be a therapeutic option for the management of high blood pressure in man. There have been relatively few studies of ACE2, either at the gene or the circulating level in patients with hypertension. Plasma ACE2 activity is low in healthy subjects, but elevated in patients with cardiovascular risk factors or cardiovascular disease. Genetic studies have investigated ACE2 gene polymorphisms with either hypertension or blood pressure, and have produced largely inconsistent findings. This review discusses the evidence regarding ACE2 in experimental hypertension models and the association between circulating ACE2 activity and ACE2 polymorphisms with blood pressure and arterial

  18. Shortwave Radiative Fluxes, Solar-Beam Transmissions, and Aerosol Properties: TARFOX and ACE-2 Find More Absorption from Flux Radiometry than from Other Measurements

    NASA Technical Reports Server (NTRS)

    Russell, Philip B.; Redemann, J.; Schmid, B.; Livingston, J. M.; Bergstrom, R. W.; Ramirez, S. A.; Hipskind, R. Stephen (Technical Monitor)

    2001-01-01

    The Tropospheric Aerosol Radiative Forcing Observational Experiment (TARFOX) and the Second Aerosol Characterization Experiment (ACE-2) made simultaneous measurements of shortwave radiative fluxes, solar-beam transmissions, and the aerosols affecting those fluxes and transmissions. Besides the measured fluxes and transmissions, other obtained properties include aerosol scattering and absorption measured in situ at the surface and aloft; aerosol single scattering albedo retrieved from skylight radiances; and aerosol complex refractive index derived by combining profiles of backscatter, extinction, and size distribution. These measurements of North Atlantic boundary layer aerosols impacted by anthropogenic pollution revealed the following characteristic results: (1) Better agreement among different types of remote measurements of aerosols (e.g., optical depth, extinction, and backscattering from sunphotometers, satellites, and lidars) than between remote and in situ measurements; 2) More extinction derived from transmission measurements than from in situ measurements; (3) Larger aerosol absorption inferred from flux radiometry than from other measurements. When the measured relationships between downwelling flux and optical depth (or beam transmission) are used to derive best-fit single scattering albedos for the polluted boundary layer aerosol, both TARFOX and ACE-2 yield midvisible values of 0.90 +/- 0.04. The other techniques give larger single scattering albedos (i.e. less absorption) for the polluted boundary layer, with a typical result of 0.95 +/- 0.04. Although the flux-based results have the virtue of describing the column aerosol unperturbed by sampling, they are subject to questions about representativeness and other uncertainties (e.g., unknown gas absorption). Current uncertainties in aerosol single scattering albedo are large in terms of climate effects. They also have an important influence on aerosol optical depths retrieved from satellite radiances

  19. Effects of ACE2 deficiency on physical performance and physiological adaptations of cardiac and skeletal muscle to exercise.

    PubMed

    Motta-Santos, Daisy; Dos Santos, Robson Augusto Souza; Oliveira, Marilene; Qadri, Fatimunnisa; Poglitsch, Marko; Mosienko, Valentina; Kappes Becker, Lenice; Campagnole-Santos, Maria Jose; M Penninger, Joseph; Alenina, Natalia; Bader, Michael

    2016-07-01

    The renin-angiotensin system (RAS) is related to physiological adaptations induced by exercise. Angiotensin-converting enzyme (ACE) 2 is a major regulator of the RAS in tissues, as it metabolizes angiotensin (Ang) II to Ang-(1-7). The aim of this study was to determine the effects of ACE2 deficiency on physical performance and physiological adaptations induced by voluntary running. Physical performance, body composition and plasma angiotensin levels, as well as tissue morphology and gene expression of RAS components in the left ventricle (LV) and skeletal muscle (gastrocnemius), were evaluated in ACE2-deficient (ACE2(-/y)) and wild-type (ACE2(+/y)) mice after 6 weeks of voluntary wheel running. ACE2(-/y) mice run less than ACE2(+/y) mice (19±4.7 vs. 26±12.6 revolutions per day × 100, P<0.01). The ACE2(+/y) group presented a lower fat mass (15±1.1%) and higher muscle mass (76.6±1.6%) after 6 weeks of voluntary running compared with the sedentary control group (fat mass: 18.3±2.1%; muscle mass: 72.7±2.2). However, no change in body composition was observed in ACE2(-/y) mice after exercise. Heart and skeletal muscle hypertrophy was observed only in trained ACE2(+/y) mice. Besides a small decrease in Ang I in ACE2(-/y) mice, plasma levels of angiotensin peptides remained unchanged by exercise or ACE2 deficiency. In the LV of trained animals, AT2 gene expression was higher in ACE2(+/y) compared with ACE2(-/y) mice. ACE2 deficiency leads to an increase in AT1 gene expression in skeletal muscle. ACE expression in soleus was increased in all exercised groups. ACE2 deficiency affects physical performance and impairs cardiac and skeletal muscle adaptations to exercise. PMID:27053009

  20. ACE2: Angiotensin II/Angiotensin-(1-7) balance in cardiorenal injury

    PubMed Central

    Varagic, Jasmina; Ahmad, Sarfaraz; Nagata, Sayaka; Ferrario, Carlos M.

    2014-01-01

    Our current recognition of the renin-angiotensin system is more convoluted than originally thought due to the discovery of multiple novel enzymes, peptides, and receptors inherent to this interactive biochemical cascade. Over the last decade angiotensin converting enzyme 2 (ACE2) has emerged as a key player in the pathophysiology of hypertension and cardiovascular and renal disease due to its pivotal role in metabolizing vasoconstrictive/hypertrophic/proliferative angiotensin II into favorable angiotensin-(1-7). This review addresses a considerable advancement in research on the role of tissue ACE2 in development and progression of hypertension and cardiorenal injury. We also summarize the results from recent clinical and experimental studies suggesting that serum or urine soluble ACE2 may serve as a novel biomarker or independent risk factor relevant for diagnosis and prognosis of cardiorenal disease. Recent proceedings on novel therapeutic approaches to enhance ACE2/angiotensin-(1-7) axis are also reviewed. PMID:24510672

  1. Crosstalk between ACE2 and PLGF regulates vascular permeability during acute lung injury

    PubMed Central

    Wang, Lantao; Li, Yong; Qin, Hao; Xing, Dong; Su, Jie; Hu, Zhenjie

    2016-01-01

    Angiotensin converting enzyme 2 (ACE2) treatment suppresses the severity of acute lung injury (ALI), through antagonizing hydrolyzing angiotensin II (AngII) and the ALI-induced apoptosis of pulmonary endothelial cells. Nevertheless, the effects of ACE2 on vessel permeability and its relationship with placental growth factor (PLGF) remain ill-defined. In the current study, we examined the relationship between ACE2 and PLGF in ALI model in mice. We used a previously published bleomycin method to induce ALI in mice, and treated the mice with ACE2. We analyzed the levels of PLGF in these mice. The mouse lung vessel permeability was determined by a fluorescence pharmacokinetic assay following i.v. injection of 62.5 µg/kg Visudyne. PLGF pump or soluble Flt-1 (sFlt-1) pump was given to augment or suppress PLGF effects, respectively. The long-term effects on lung function were determined by measurement of lung resistance using methacholine. We found that ACE2 treatment did not alter PLGF levels in lung, but antagonized the effects of PLGF on increases of lung vessel permeability. Ectogenic PLGF abolished the antagonizing effects of ACE2 on the vessel permeability against PLGF. On the other hand, suppression of PLGF signaling mimicked the effects of ACE2 on the vessel permeability against PLGF. The suppression of vessel permeability resulted in improvement of lung function after ALI. Thus, ACE2 may antagonize the PLGF-mediated increases in lung vessel permeability during ALI, resulting in improvement of lung function after ALI. PMID:27158411

  2. Developmental expression of ACE2 in the SHR kidney: a role in hypertension?

    PubMed

    Tikellis, C; Cooper, M E; Bialkowski, K; Johnston, C I; Burns, W C; Lew, R A; Smith, A I; Thomas, M C

    2006-07-01

    The abnormal development of the intrarenal renin-angiotensin system (RAS) is thought contribute to adult-onset hypertension in the spontaneously hypertensive rat (SHR). Angiotensin-converting enzyme 2 (ACE2) is a novel enzyme with complementary actions to that of ACE. Recent studies have shown that ACE2 expression is reduced in the adult SHR. However, its regulation in pre-hypertensive animals is unknown. In this study, we examine the developmental expression of ACE2 in the rodent kidney and its temporal expression, as it relates to the development of hypertension in the SHR model. Kidneys from SHR and normotensive Wistar Kyoto (WKY) rats (n=8-12/group) at birth, 6 weeks of age, and adulthood (80 days) were examined. Gene expression and activity of ACE2 were determined by real-time reverse transcription-polymerase chain reaction and quenched fluorescence assays, respectively. Renal expression was localized by in situ hybridization and immunohistochemistry. The expression and ACE2 activity are significantly increased in the SHR kidney at birth. With the onset of hypertension, the tubular expression of ACE2 falls in SHR compared to WKY and remains reduced in the adult SHR kidney. Glomerular expression is paradoxically increased in the SHR glomerulus. The overall developmental pattern of ACE2 expression in the SHR kidney is also modified, with declining expression over the course of renal development. The developmental pattern of ACE2 expression in the SHR kidney is altered before the onset of hypertension, consistent with the key role of the RAS in the pathogenesis of adult-onset hypertension. Further research is required to distinguish the contribution of these changes to the development and progression of hypertension in this model. PMID:16710353

  3. ACE2 overexpression inhibits acquired platinum resistance-induced tumor angiogenesis in NSCLC.

    PubMed

    Cheng, Qijian; Zhou, Ling; Zhou, Jianping; Wan, Huanying; Li, Qingyun; Feng, Yun

    2016-09-01

    Angiotensin II (AngII) is a multifunctional bioactive peptide in the renin-angiotensin system (RAS). Angiotensin-converting enzyme 2 (ACE2) is a newly identified component of RAS. We previously reported that ACE2 overexpression may inhibit cell growth and vascular endothelial growth factor (VEGF) production in vitro and in vivo. In the present study, we investigated the effect of ACE2 on tumor-associated angiogen-esis after the development of acquired platinum resistance in non-small cell lung cancer (NSCLC). Four NSCLC cell lines, A549, LLC, A549-DDP and LLC-DDP, were used in vitro, while A549 and A549-DDP cells were used in vivo. A549-DDP and LLC-DDP cells were newly established at our institution as acquired platinum-resistant sublines by culturing the former parent cells in cisplatin (CDDP)-containing conditioned medium for 6 months. These platinum-resistant cells showed significantly higher angiotensin II type 1 receptor (AT1R), ACE and VEGF production and lower ACE2 expression than their corresponding parent cells. We showed that ACE2 overexpression inhibited the production of VEGF in vitro and in vivo compared to their corresponding parent cells. We also found that ACE2 overexpression reduced the expression of AT1R and ACE. Additionally, we confirmed that ACE2 overexpres-sion inhibited cell growth and VEGF production while simultaneously suppressing ACE and AT1R expression in human lung cancer xenografts. Our findings indicate that ACE2 overexpression may potentially suppress angiogenesis in NSCLC after the development of acquired platinum resistance. PMID:27460845

  4. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2

    PubMed Central

    Li, Wenhui; Zhang, Chengsheng; Sui, Jianhua; Kuhn, Jens H; Moore, Michael J; Luo, Shiwen; Wong, Swee-Kee; Huang, I-Chueh; Xu, Keming; Vasilieva, Natalya; Murakami, Akikazu; He, Yaqing; Marasco, Wayne A; Guan, Yi; Choe, Hyeryun; Farzan, Michael

    2005-01-01

    Human angiotensin-converting enzyme 2 (ACE2) is a functional receptor for SARS coronavirus (SARS-CoV). Here we identify the SARS-CoV spike (S)-protein-binding site on ACE2. We also compare S proteins of SARS-CoV isolated during the 2002–2003 SARS outbreak and during the much less severe 2003–2004 outbreak, and from palm civets, a possible source of SARS-CoV found in humans. All three S proteins bound to and utilized palm-civet ACE2 efficiently, but the latter two S proteins utilized human ACE2 markedly less efficiently than did the S protein obtained during the earlier human outbreak. The lower affinity of these S proteins could be complemented by altering specific residues within the S-protein-binding site of human ACE2 to those of civet ACE2, or by altering S-protein residues 479 and 487 to residues conserved during the 2002–2003 outbreak. Collectively, these data describe molecular interactions important to the adaptation of SARS-CoV to human cells, and provide insight into the severity of the 2002–2003 SARS epidemic. PMID:15791205

  5. Regulation of Ace2-dependent genes requires components of the PBF complex in Schizosaccharomyces pombe

    PubMed Central

    Suárez, M Belén; Alonso-Nuñez, María Luisa; del Rey, Francisco; McInerny, Christopher J; Vázquez de Aldana, Carlos R

    2015-01-01

    The division cycle of unicellular yeasts is completed with the activation of a cell separation program that results in the dissolution of the septum assembled during cytokinesis between the 2 daughter cells, allowing them to become independent entities. Expression of the eng1+ and agn1+ genes, encoding the hydrolytic enzymes responsible for septum degradation, is activated at the end of each cell cycle by the transcription factor Ace2. Periodic ace2+ expression is regulated by the transcriptional complex PBF (PCB Binding Factor), composed of the forkhead-like proteins Sep1 and Fkh2 and the MADS box-like protein Mbx1. In this report, we show that Ace2-dependent genes contain several combinations of motifs for Ace2 and PBF binding in their promoters. Thus, Ace2, Fkh2 and Sep1 were found to bind in vivo to the eng1+ promoter. Ace2 binding was coincident with maximum level of eng1+ expression, whereas Fkh2 binding was maximal when mRNA levels were low, supporting the notion that they play opposing roles. In addition, we found that the expression of eng1+ and agn1+ was differentially affected by mutations in PBF components. Interestingly, agn1+ was a major target of Mbx1, since its ectopic expression resulted in the suppression of Mbx1 deletion phenotypes. Our results reveal a complex regulation system through which the transcription factors Ace2, Fkh2, Sep1 and Mbx1 in combination control the expression of the genes involved in separation at the end of the cell division cycle. PMID:26237280

  6. Regulation of Ace2-dependent genes requires components of the PBF complex in Schizosaccharomyces pombe.

    PubMed

    Suárez, M Belén; Alonso-Nuñez, María Luisa; del Rey, Francisco; McInerny, Christopher J; Vázquez de Aldana, Carlos R

    2015-01-01

    The division cycle of unicellular yeasts is completed with the activation of a cell separation program that results in the dissolution of the septum assembled during cytokinesis between the 2 daughter cells, allowing them to become independent entities. Expression of the eng1(+) and agn1(+) genes, encoding the hydrolytic enzymes responsible for septum degradation, is activated at the end of each cell cycle by the transcription factor Ace2. Periodic ace2(+) expression is regulated by the transcriptional complex PBF (PCB Binding Factor), composed of the forkhead-like proteins Sep1 and Fkh2 and the MADS box-like protein Mbx1. In this report, we show that Ace2-dependent genes contain several combinations of motifs for Ace2 and PBF binding in their promoters. Thus, Ace2, Fkh2 and Sep1 were found to bind in vivo to the eng1(+) promoter. Ace2 binding was coincident with maximum level of eng1(+) expression, whereas Fkh2 binding was maximal when mRNA levels were low, supporting the notion that they play opposing roles. In addition, we found that the expression of eng1(+) and agn1(+) was differentially affected by mutations in PBF components. Interestingly, agn1(+) was a major target of Mbx1, since its ectopic expression resulted in the suppression of Mbx1 deletion phenotypes. Our results reveal a complex regulation system through which the transcription factors Ace2, Fkh2, Sep1 and Mbx1 in combination control the expression of the genes involved in separation at the end of the cell division cycle. PMID:26237280

  7. RAM: A Conserved Signaling Network That Regulates Ace2p Transcriptional Activity and Polarized MorphogenesisD⃞

    PubMed Central

    Nelson, Bryce; Kurischko, Cornelia; Horecka, Joe; Mody, Manali; Nair, Pradeep; Pratt, Lana; Zougman, Alexandre; McBroom, Linda D.B.; Hughes, Timothy R.; Boone, Charlie; Luca, Francis C.

    2003-01-01

    In Saccharomyces cerevisiae, polarized morphogenesis is critical for bud site selection, bud development, and cell separation. The latter is mediated by Ace2p transcription factor, which controls the daughter cell-specific expression of cell separation genes. Recently, a set of proteins that include Cbk1p kinase, its binding partner Mob2p, Tao3p (Pag1p), and Hym1p were shown to regulate both Ace2p activity and cellular morphogenesis. These proteins seem to form a signaling network, which we designate RAM for regulation of Ace2p activity and cellular morphogenesis. To find additional RAM components, we conducted genetic screens for bilateral mating and cell separation mutants and identified alleles of the PAK-related kinase Kic1p in addition to Cbk1p, Mob2p, Tao3p, and Hym1p. Deletion of each RAM gene resulted in a loss of Ace2p function and caused cell polarity defects that were distinct from formin or polarisome mutants. Two-hybrid and coimmunoprecipitation experiments reveal a complex network of interactions among the RAM proteins, including Cbk1p–Cbk1p, Cbk1p–Kic1p, Kic1p–Tao3p, and Kic1p–Hym1p interactions, in addition to the previously documented Cbk1p–Mob2p and Cbk1p–Tao3p interactions. We also identified a novel leucine-rich repeat-containing protein Sog2p that interacts with Hym1p and Kic1p. Cells lacking Sog2p exhibited the characteristic cell separation and cell morphology defects associated with perturbation in RAM signaling. Each RAM protein localized to cortical sites of growth during both budding and mating pheromone response. Hym1p was Kic1p- and Sog2p-dependent and Sog2p and Kic1p were interdependent for localization, indicating a close functional relationship between these proteins. Only Mob2p and Cbk1p were detectable in the daughter cell nucleus at the end of mitosis. The nuclear localization and kinase activity of the Mob2p–Cbk1p complex were dependent on all other RAM proteins, suggesting that Mob2p–Cbk1p functions late in the

  8. Regional Aerosol Optical Depth Characteristics from Satellite Observations: ACE-1, TARFOX and ACE-2 Results

    NASA Technical Reports Server (NTRS)

    Durkee, P. A.; Nielsen, K. E.; Smith, P. J.; Russell, P. B.; Schmid, B.; Livingston, J. M.; Holben, B. N.; Tomasi, C.; Vitale, V.; Collins, D.

    1999-01-01

    Analysis of the aerosol properties during 3 recent international field campaigns ACE-1, TARFOX and ACE-2 are described using satellite retrievals from NOAA AVHRR data. Validation of the satellite retrieval procedure is performed with airborne, shipboard, and land-based sunphotometry during ACE-2. The intercomparison between satellite and surface optical depths has a correlation coefficient of 0.93 for 630 nm wavelength and 0.92 for 860 nm wavelength, The standard error of estimate is 0.025 for 630 nm wavelength and 0.023 for 860 nm wavelength. Regional aerosol properties are examined in composite analysis of aerosol optical properties from the ACE-1, TARFOX and ACE-2 regions. ACE-1 and ACE-2 regions have strong modes in the distribution of optical depth around 0.1, but the ACE-2 tails toward higher values yielding an average of 0.16 consistent with pollution and dust aerosol intrusions. The TARFOX region has a noticeable mode of 0.2, but has significant spread of aerosol optical depth values consistent with the varied continental aerosol constituents off the eastern North American Coast.

  9. Cell cycle dependence of ACE-2 explains downregulation in idiopathic pulmonary fibrosis.

    PubMed

    Uhal, Bruce D; Dang, MyTrang; Dang, Vinh; Llatos, Roger; Cano, Esteban; Abdul-Hafez, Amal; Markey, Jonathan; Piasecki, Christopher C; Molina-Molina, Maria

    2013-07-01

    Alveolar epithelial type II cells, a major source of angiotensin-converting enzyme (ACE)-2 in the adult lung, are normally quiescent but actively proliferate in lung fibrosis and downregulate this protective enzyme. It was, therefore, hypothesised that ACE-2 expression might be related to cell cycle progression. To test this hypothesis, ACE-2 mRNA levels, protein levels and enzymatic activity were examined in fibrotic human lungs and in the alveolar epithelial cell lines A549 and MLE-12 studied at postconfluent (quiescent) versus subconfluent (proliferating) densities. ACE-2 mRNA, immunoreactive protein and enzymatic activity were all high in quiescent cells, but were severely downregulated or absent in actively proliferating cells. Upregulation of the enzyme in cells that were progressing to quiescence was completely inhibited by the transcription blocker actinomycin D or by SP600125, an inhibitor of c-Jun N-terminal kinase (JNK). In lung biopsy specimens obtained from patients with idiopathic pulmonary fibrosis, immunoreactive enzyme was absent in alveolar epithelia that were positive for proliferation markers, but was robustly expressed in alveolar epithelia devoid of proliferation markers. These data explain the loss of ACE-2 in lung fibrosis and demonstrate cell cycle-dependent regulation of this protective enzyme by a JNK-mediated transcriptional mechanism. PMID:23100504

  10. Oral Delivery of ACE2/Ang-(1–7) Bioencapsulated in Plant Cells Protects against Experimental Uveitis and Autoimmune Uveoretinitis

    PubMed Central

    Shil, Pollob K; Kwon, Kwang-Chul; Zhu, Ping; Verma, Amrisha; Daniell, Henry; Li, Qiuhong

    2014-01-01

    Hyperactivity of the renin-angiotensin system (RAS) resulting in elevated Angiotensin II (Ang II) contributes to all stages of inflammatory responses including ocular inflammation. The discovery of angiotensin-converting enzyme 2 (ACE2) has established a protective axis of RAS involving ACE2/Ang-(1–7)/Mas that counteracts the proinflammatory and hypertrophic effects of the deleterious ACE/AngII/AT1R axis. Here we investigated the hypothesis that enhancing the systemic and local activity of the protective axis of the RAS by oral delivery of ACE2 and Ang-(1–7) bioencapsulated in plant cells would confer protection against ocular inflammation. Both ACE2 and Ang-(1–7), fused with the non-toxic cholera toxin subunit B (CTB) were expressed in plant chloroplasts. Increased levels of ACE2 and Ang-(1–7) were observed in circulation and retina after oral administration of CTB-ACE2 and Ang-(1–7) expressing plant cells. Oral feeding of mice with bioencapsulated ACE2/Ang-(1–7) significantly reduced endotoxin-induced uveitis (EIU) in mice. Treatment with bioencapsulated ACE2/Ang-(1–7) also dramatically decreased cellular infiltration, retinal vasculitis, damage and folding in experimental autoimmune uveoretinitis (EAU). Thus, enhancing the protective axis of RAS by oral delivery of ACE2/Ang-(1–7) bioencapsulated in plant cells provide an innovative, highly efficient and cost-effective therapeutic strategy for ocular inflammatory diseases. PMID:25228068

  11. ACE2 Deficiency Worsens Epicardial Adipose Tissue Inflammation and Cardiac Dysfunction in Response to Diet-Induced Obesity.

    PubMed

    Patel, Vaibhav B; Mori, Jun; McLean, Brent A; Basu, Ratnadeep; Das, Subhash K; Ramprasath, Tharmarajan; Parajuli, Nirmal; Penninger, Josef M; Grant, Maria B; Lopaschuk, Gary D; Oudit, Gavin Y

    2016-01-01

    Obesity is increasing in prevalence and is strongly associated with metabolic and cardiovascular disorders. The renin-angiotensin system (RAS) has emerged as a key pathogenic mechanism for these disorders; angiotensin (Ang)-converting enzyme 2 (ACE2) negatively regulates RAS by metabolizing Ang II into Ang 1-7. We studied the role of ACE2 in obesity-mediated cardiac dysfunction. ACE2 null (ACE2KO) and wild-type (WT) mice were fed a high-fat diet (HFD) or a control diet and studied at 6 months of age. Loss of ACE2 resulted in decreased weight gain but increased glucose intolerance, epicardial adipose tissue (EAT) inflammation, and polarization of macrophages into a proinflammatory phenotype in response to HFD. Similarly, human EAT in patients with obesity and heart failure displayed a proinflammatory macrophage phenotype. Exacerbated EAT inflammation in ACE2KO-HFD mice was associated with decreased myocardial adiponectin, decreased phosphorylation of AMPK, increased cardiac steatosis and lipotoxicity, and myocardial insulin resistance, which worsened heart function. Ang 1-7 (24 µg/kg/h) administered to ACE2KO-HFD mice resulted in ameliorated EAT inflammation and reduced cardiac steatosis and lipotoxicity, resulting in normalization of heart failure. In conclusion, ACE2 plays a novel role in heart disease associated with obesity wherein ACE2 negatively regulates obesity-induced EAT inflammation and cardiac insulin resistance. PMID:26224885

  12. Tissue specific up regulation of ACE2 in rabbit model of atherosclerosis by atorvastatin: role of epigenetic histone modifications.

    PubMed

    Tikoo, Kulbhushan; Patel, Gaurang; Kumar, Sandeep; Karpe, Pinakin Arun; Sanghavi, Maitri; Malek, Vajir; Srinivasan, K

    2015-02-01

    Growing body of evidence points out the crucial role of ACE2 in preventing atherosclerosis. However, data on how atherosclerosis affects ACE2 expression in heart and kidney remains unknown. Atherosclerosis was induced by feeding New Zealand White rabbits with high cholesterol diet (HCD - 2%) for 12 weeks and atorvastatin was administered (5mg/kg/day p.o) in last 3 weeks. ACE2 mRNA and protein expression was assessed by Western blotting and real time PCR. HCD fed rabbits developed atherosclerosis as confirmed by increase in plasma total cholesterol, LDL and triglycerides as well as formation atherosclerotic plaques in arch of aorta. The ACE2 protein but not mRNA expression was reduced in heart and kidney of HCD rabbits. Interestingly, atorvastatin increased the ACE2 protein expression in heart and kidney of HCD rabbits. However, atorvastatin increased ACE2 mRNA in heart but not in kidney of HCD rabbits. Atorvastatin increased the occupancy of histone H3 acetylation (H3-Ac) mark on ACE2 promoter region in heart of HCD rabbits indicating direct or indirect epigenetic up-regulation of ACE2 by atorvastatin. Further, atorvastatin suppressed Ang II-induced contractile responses and enhanced AT2 receptor mediated relaxant responses in atherosclerotic aorta. We propose that atherosclerosis is associated with reduced ACE2 expression in heart and kidney. We also show an unexplored potential of atorvastatin to up-regulate ACE2 via epigenetic histone modifications. Our data suggest a novel way of replenishing ACE2 expression for preventing not only atherosclerosis but also other cardiovascular disorders. PMID:25482567

  13. ACE2 and vasoactive peptides: novel players in cardiovascular/renal remodeling and hypertension.

    PubMed

    Mendoza-Torres, Evelyn; Oyarzún, Alejandra; Mondaca-Ruff, David; Azocar, Andrés; Castro, Pablo F; Jalil, Jorge E; Chiong, Mario; Lavandero, Sergio; Ocaranza, María Paz

    2015-08-01

    The renin-angiotensin system (RAS) is a key component of cardiovascular physiology and homeostasis due to its influence on the regulation of electrolyte balance, blood pressure, vascular tone and cardiovascular remodeling. Deregulation of this system contributes significantly to the pathophysiology of cardiovascular and renal diseases. Numerous studies have generated new perspectives about a noncanonical and protective RAS pathway that counteracts the proliferative and hypertensive effects of the classical angiotensin-converting enzyme (ACE)/angiotensin (Ang) II/angiotensin type 1 receptor (AT1R) axis. The key components of this pathway are ACE2 and its products, Ang-(1-7) and Ang-(1-9). These two vasoactive peptides act through the Mas receptor (MasR) and AT2R, respectively. The ACE2/Ang-(1-7)/MasR and ACE2/Ang-(1-9)/AT2R axes have opposite effects to those of the ACE/Ang II/AT1R axis, such as decreased proliferation and cardiovascular remodeling, increased production of nitric oxide and vasodilation. A novel peptide from the noncanonical pathway, alamandine, was recently identified in rats, mice and humans. This heptapeptide is generated by catalytic action of ACE2 on Ang A or through a decarboxylation reaction on Ang-(1-7). Alamandine produces the same effects as Ang-(1-7), such as vasodilation and prevention of fibrosis, by interacting with Mas-related GPCR, member D (MrgD). In this article, we review the key roles of ACE2 and the vasoactive peptides Ang-(1-7), Ang-(1-9) and alamandine as counter-regulators of the ACE-Ang II axis as well as the biological properties that allow them to regulate blood pressure and cardiovascular and renal remodeling. PMID:26275770

  14. Influence of Humidity on the Aerosol Scattering Coefficient and Its Effect on the Upwelling Radiance During ACE-2

    NASA Technical Reports Server (NTRS)

    Gasso, B. S.; Hegg, D. A.; Covert, D. S.; Collins, D.; Noone, K.; Oestroem, E.; Schmid, B.; Russell, P. B.; Livingston, J. M.; Durkee, P. A.; Jonsson, H.

    2000-01-01

    Aerosol scattering coefficients (sigma(sub sp)) have been measured over the ocean at different relative humidities (RH) as a function of attitude in the region surrounding the Canary Islands during the Second Aerosol Characterization Experiment (ACE-2) in June and July 1997. The data were collected by the University of Washington passive humidigraph (UWPH) mounted on the Pelican research aircraft. Concurrently, particle size distributions absorption coefficients and aerosol optical depth were measured throughout 17 flights. A parameterization of sigma(sub sp) as a function of RH was utilized to assess the impact of aerosol hydration on the upwelling radiance (normalized to the solar constant and cosine of zenith angle). The top of the atmosphere radiance signal was simulated at wavelengths corresponding to visible and near-infrared bands or the EOS-AM ("Terra") detectors, MODIS and MISR. The UWPH measured (sigma(sub sp)) at 2 RHs, one below and the other above ambient conditions. Ambient (sigma(sub sp)) was obtained by interpolation of these 2 measurements. The data were stratified in terms of 3 types of aerosols: Saharan dust, clean marine (marine boundary layer background) and polluted marine aerosols (i.e., 2- or 1-day old polluted aerosols advected from Europe). An empirical relation for the dependence of (sigma(sub sp)) on RH, defined by (sigma(sub sp))(RH) = k. ((1 - RH/100)(exp -gamma), was used with the hygroscopic exponent gamma derived from the data. The following gamma values were obtained for the 3 aerosol types: gamma(dust) = 0.23 +/- 0.05, gamma(clean marine) = 0.69 +/- 0.06 and gamma(polluted marine) = 0.57 + 0.06. Based on the measured (gamma)(s), the above equation was utilized to derive aerosol models with different hygroscopicities. The satellite simulation signal code 6S was used to compute the upwelling radiance corresponding to each of those aerosol models at several ambient humidities. For the pre-launch estimated precision of the sensors and

  15. Influence of Humidity On the Aerosol Scattering Coefficient and Its Effect on the Upwelling Radiance During ACE-2

    NASA Technical Reports Server (NTRS)

    Gasso, S.; Hegg, D. A.; Covert, D. S.; Collins, D.; Noone, K. J.; Oestroem, E.; Schmid, B.; Russell, P. B.; Livingston, J. M.; Durkee, P. A.

    2000-01-01

    Aerosol scattering coefficients (sigma(sub sp)) have been measured over the ocean at different relative humidities (RH) as a function of altitude in the region surrounding the Canary Islands during the Second Aerosol Characterization Experiment (ACE-2) in June and July 1997. The data were collected by the University of Washington passive humidigraph (UWPH) mounted on the Pelican research aircraft. Concurrently, particle size distributions, absorption coefficients and aerosol optical depth were measured throughout 17 flights. A parameterization of sigma(sub sp) as a function of RH was utilized to assess the impact of aerosol hydration on the upwelling radiance (normalized to the solar constant and cosine of zenith angle). The top of the atmosphere radiance signal was simulated at wavelengths corresponding to visible and near-infrared bands of the EOS (Earth Observing System) AM-1 (Terra) detectors, MODIS (Moderate Resolution Imaging Spectroradiometer) and MISR (Multi-angle Imaging Spectroradiometer). The UWPH measured sigma(sub sp) at two RHs, one below and the other above ambient conditions. Ambient sigma(sub sp) was obtained by interpolation of these two measurements. The data were stratified in terms of three types of aerosols: Saharan dust, clean marine (marine boundary layer background) and polluted marine aerosols (i.e., two- or one-day old polluted aerosols advected from Europe). An empirical relation for the dependence of sigma(sub sp) on RH, defined by sigma(sub sp)(RH) = k.(1 - RH/100)(sup gamma), was used with the hygroscopic exponent gamma derived from the data. The following gamma values were obtained for the 3 aerosol types: gamma(dust) = 0.23 +/- 0.05, gamma(clean marine) = 0.69 +/- 0.06 and gamma(polluted marine) = 0.57 +/- 0.06. Based on the measured gammas, the above equation was utilized to derive aerosol models with different hygroscopicities. The satellite simulation signal code 6S was used to compute the upwelling radiance corresponding to each

  16. North Atlantic Aerosol Radiative Effects Based on Satellite Measurements and Aerosol Intensive Properties from TARFOX and ACE-2

    NASA Technical Reports Server (NTRS)

    Bergstrom, Robert W.; Russell, Philip B.

    2000-01-01

    We estimate the impact of North Atlantic aerosols on the net shortwave flux at the tropopause by combining maps of satellite-derived aerosol optical depth (AOD) with model aerosol properties. We exclude African dust, primarily by restricting latitudes to 25-60 N. Aerosol properties were determined via column closure analyses in two recent experiments, TARFOX and ACE 2. The analyses use in situ measurements of aerosol composition and air- and ship-borne sunphotometer measurements of AOD spectra. The resulting aerosol model yields computed flux sensitivities (dFlux/dAOD) that agree with measurements by airborne flux radiometers in TARFOX. It has a midvisible single-scattering albedo of 0.9, which is in the range obtained from in situ measurements of aerosol scattering and absorption in both TARFOX and ACE 2. Combining seasonal maps of AVHRR-derived midvisible AOD with the aerosol model yields maps of 24-hour average net radiative flux changes at the tropopause. For cloud-free conditions, results range from -9 W/sq m near the eastern US coastline in the summer to -1 W/sq m in the mid-Atlantic during winter; the regional annual average is -3.5 W/sq m. Using a non- absorbing aerosol model increases these values by about 30%. We estimate the effect of clouds using ISCCP cloud-fraction maps. Because ISCCP midlatitude North Atlantic cloud fractions are relatively large, they greatly reduce the computed aerosol-induced flux changes. For example, the regional annual average decreases from -3.5 W/sq m to -0.8 W/sq m. We compare results to previous model calculations for a variety of aerosol types.

  17. Regulation of alveolar epithelial cell survival by the ACE-2/angiotensin 1-7/Mas axis.

    PubMed

    Uhal, Bruce D; Li, Xiaopeng; Xue, Anita; Gao, Xu; Abdul-Hafez, Amal

    2011-09-01

    Earlier work from this laboratory demonstrated that apoptosis of alveolar epithelial cells (AECs) requires autocrine generation of angiotensin (ANG) II. More recent studies showed that angiotensin converting enzyme-2 (ACE-2), which degrades ANGII to form ANG1-7, is protective but severely downregulated in human and experimental lung fibrosis. Here it was theorized that ACE-2 and its product ANG1-7 might therefore regulate AEC apoptosis. To evaluate this hypothesis, the AEC cell line MLE-12 and primary cultures of rat AECs were exposed to the profibrotic apoptosis inducers ANGII or bleomycin (Bleo). Markers of apoptosis (caspase-9 or -3 activation and nuclear fragmentation), steady-state ANGII and ANG1-7, and JNK phosphorylation were measured thereafter. In the absence of Bleo, inhibition of ACE-2 by small interfering RNA or by a competitive inhibitor (DX600 peptide) caused a reciprocal increase in autocrine ANGII and corresponding decrease in ANG1-7 in cell culture media (both P < 0.05) and, moreover, induced AEC apoptosis. At baseline (without inhibitor), ANG1-7 in culture media was 10-fold higher than ANGII (P < 0.01). Addition of purified ANGII or bleomycin-induced caspase activation, nuclear fragmentation, and JNK phosphorylation in cultured AECs. However, preincubation with ANG1-7 (0.1 μM) prevented JNK phosphorylation and apoptosis. Moreover, pretreatment with A779, a specific blocker of the ANG1-7 receptor mas, prevented ANG1-7 blockade of JNK phosphorylation, caspase activation, and nuclear fragmentation. These data demonstrate that ACE-2 regulates AEC survival by balancing the proapoptotic ANGII and its antiapoptotic degradation product ANG1-7. They also suggest that ANG1-7 inhibits AEC apoptosis through the ANG1-7 receptor mas. PMID:21665960

  18. Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus.

    PubMed

    Xu, Lili; Zhang, Yanfang; Liu, Yun; Chen, Zhiwei; Deng, Hongkui; Ma, Zhongbin; Wang, Hualin; Hu, Zhihong; Deng, Fei

    2009-11-01

    Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4 % to ACE2 proteins from dog, masked palm civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and masked palm civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of masked palm civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of masked palm civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks. PMID:19625462

  19. ACE2 deficiency reduces β-cell mass and impairs β-cell proliferation in obese C57BL/6 mice.

    PubMed

    Shoemaker, Robin; Yiannikouris, Frederique; Thatcher, Sean; Cassis, Lisa

    2015-10-01

    Drugs that inhibit the renin-angiotensin system (RAS) decrease the onset of type 2 diabetes (T2D). Pancreatic islets express RAS components, including angiotensin-converting enzyme 2 (ACE2), which cleaves angiotensin II (Ang II) to angiotensin-(1-7) [Ang-(1-7)]. Overexpression of ACE2 in pancreas of diabetic mice improved glucose homeostasis. The purpose of this study was to determine if deficiency of endogenous ACE2 contributes to islet dysfunction and T2D. We hypothesized that ACE2 deficiency potentiates the decline in β-cell function and augments the development of diet-induced T2D. Male Ace2(+/y) or Ace2(-/y) mice were fed a low-fat (LF) or high-fat (HF) diet for 1 or 4 mo. A subset of 1-mo HF-fed mice were infused with Sal (Sal), losartan (Los), or Ang-(1-7). At 4 mo, while both genotypes of HF-fed mice developed a similar level of insulin resistance, adaptive hyperinsulinemia was reduced in Ace2(-/y) vs. Ace2(+/y) mice. Similarly, in vivo glucose-stimulated insulin secretion (GSIS) was reduced in 1-mo HF-fed Ace2(-/y) compared with Ace2(+/y) mice, resulting in augmented hyperglycemia. The average islet area was significantly smaller in both LF- and HF-fed Ace2(-/y) vs. Ace2(+/y) mice. Additionally, β-cell mass and proliferation were reduced significantly in HF-fed Ace2(-/y) vs. Ace2(+/y) mice. Neither infusion of Los nor Ang-(1-7) was able to correct impaired in vivo GSIS of HF-fed ACE2-deficient mice. These results demonstrate a critical role for endogenous ACE2 in the adaptive β-cell hyperinsulinemic response to HF feeding through regulation of β-cell proliferation and growth. PMID:26389599

  20. A single nucleotide polymorphism uncovers a novel function for the transcription factor Ace2 during Candida albicans hyphal development.

    PubMed

    Calderón-Noreña, Diana M; González-Novo, Alberto; Orellana-Muñoz, Sara; Gutiérrez-Escribano, Pilar; Arnáiz-Pita, Yolanda; Dueñas-Santero, Encarnación; Suárez, M Belén; Bougnoux, Marie-Elisabeth; Del Rey, Francisco; Sherlock, Gavin; d'Enfert, Christophe; Correa-Bordes, Jaime; de Aldana, Carlos R Vázquez

    2015-04-01

    Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor is its ability to switch between the yeast and hyphal forms, and these filamentous forms are important in tissue penetration and invasion. A common feature for filamentous growth is the ability to inhibit cell separation after cytokinesis, although it is poorly understood how this process is regulated developmentally. In C. albicans, the formation of filaments during hyphal growth requires changes in septin ring dynamics. In this work, we studied the functional relationship between septins and the transcription factor Ace2, which controls the expression of enzymes that catalyze septum degradation. We found that alternative translation initiation produces two Ace2 isoforms. While full-length Ace2, Ace2L, influences septin dynamics in a transcription-independent manner in hyphal cells but not in yeast cells, the use of methionine-55 as the initiation codon gives rise to Ace2S, which functions as the nuclear transcription factor required for the expression of cell separation genes. Genetic evidence indicates that Ace2L influences the incorporation of the Sep7 septin to hyphal septin rings in order to avoid inappropriate activation of cell separation during filamentous growth. Interestingly, a natural single nucleotide polymorphism (SNP) present in the C. albicans WO-1 background and other C. albicans commensal and clinical isolates generates a stop codon in the ninth codon of Ace2L that mimics the phenotype of cells lacking Ace2L. Finally, we report that Ace2L and Ace2S interact with the NDR kinase Cbk1 and that impairing activity of this kinase results in a defect in septin dynamics similar to that of hyphal cells lacking Ace2L. Together, our findings identify Ace2L and the NDR kinase Cbk1 as new elements of the signaling system that modify septin ring dynamics in hyphae to allow cell-chain formation, a feature that appears to have evolved in specific C. albicans lineages

  1. A Single Nucleotide Polymorphism Uncovers a Novel Function for the Transcription Factor Ace2 during Candida albicans Hyphal Development

    PubMed Central

    Orellana-Muñoz, Sara; Gutiérrez-Escribano, Pilar; Arnáiz-Pita, Yolanda; Dueñas-Santero, Encarnación; Suárez, M. Belén; Bougnoux, Marie-Elisabeth; del Rey, Francisco; Sherlock, Gavin; d’Enfert, Christophe; Correa-Bordes, Jaime; de Aldana, Carlos R. Vázquez

    2015-01-01

    Candida albicans is a major invasive fungal pathogen in humans. An important virulence factor is its ability to switch between the yeast and hyphal forms, and these filamentous forms are important in tissue penetration and invasion. A common feature for filamentous growth is the ability to inhibit cell separation after cytokinesis, although it is poorly understood how this process is regulated developmentally. In C. albicans, the formation of filaments during hyphal growth requires changes in septin ring dynamics. In this work, we studied the functional relationship between septins and the transcription factor Ace2, which controls the expression of enzymes that catalyze septum degradation. We found that alternative translation initiation produces two Ace2 isoforms. While full-length Ace2, Ace2L, influences septin dynamics in a transcription-independent manner in hyphal cells but not in yeast cells, the use of methionine-55 as the initiation codon gives rise to Ace2S, which functions as the nuclear transcription factor required for the expression of cell separation genes. Genetic evidence indicates that Ace2L influences the incorporation of the Sep7 septin to hyphal septin rings in order to avoid inappropriate activation of cell separation during filamentous growth. Interestingly, a natural single nucleotide polymorphism (SNP) present in the C. albicans WO-1 background and other C. albicans commensal and clinical isolates generates a stop codon in the ninth codon of Ace2L that mimics the phenotype of cells lacking Ace2L. Finally, we report that Ace2L and Ace2S interact with the NDR kinase Cbk1 and that impairing activity of this kinase results in a defect in septin dynamics similar to that of hyphal cells lacking Ace2L. Together, our findings identify Ace2L and the NDR kinase Cbk1 as new elements of the signaling system that modify septin ring dynamics in hyphae to allow cell-chain formation, a feature that appears to have evolved in specific C. albicans lineages

  2. ACE2, angiotensin-(1-7) and Mas receptor axis in inflammation and fibrosis

    PubMed Central

    Simões e Silva, AC; Silveira, KD; Ferreira, AJ; Teixeira, MM

    2013-01-01

    Recent advances have improved our understanding of the renin-angiotensin system (RAS). These have included the recognition that angiotensin (Ang)-(1-7) is a biologically active product of the RAS cascade. The identification of the ACE homologue ACE2, which forms Ang-(1-7) from Ang II, and the GPCR Mas as an Ang-(1-7) receptor have provided the necessary biochemical and molecular background and tools to study the biological significance of Ang-(1-7). Most available evidence supports a counter-regulatory role for Ang-(1-7) by opposing many actions of Ang II on AT1 receptors, especially vasoconstriction and proliferation. Many studies have now shown that Ang-(1-7) by acting via Mas receptor exerts inhibitory effects on inflammation and on vascular and cellular growth mechanisms. Ang-(1-7) has also been shown to reduce key signalling pathways and molecules thought to be relevant for fibrogenesis. Here, we review recent findings related to the function of the ACE2/Ang-(1-7)/Mas axis and focus on the role of this axis in modifying processes associated with acute and chronic inflammation, including leukocyte influx, fibrogenesis and proliferation of certain cell types. More attention will be given to the involvement of the ACE2/Ang-(1-7)/Mas axis in the context of renal disease because of the known relevance of the RAS for the function of this organ and for the regulation of kidney inflammation and fibrosis. Taken together, this knowledge may help in paving the way for the development of novel treatments for chronic inflammatory and renal diseases. PMID:23488800

  3. No association of angiotensin-converting enzyme 2 gene (ACE2) polymorphisms with essential hypertension.

    PubMed

    Benjafield, Adam V; Wang, William Y S; Morris, Brian J

    2004-07-01

    Recent intriguing findings from genetic linkage, knockout, and physiologic studies in mice and rats led us to conduct the first investigation of the novel angiotensin-converting enzyme 2 gene (ACE2) in human hypertension (HT). We genotyped four single nucleotide polymorphisms (SNP) (A-->G at nucleotide 1075 in intron 1, G-->A at nucleotide 8790 in intron 3, C-->G at nucleotide 28330 in intron 11, and G-->C at nucleotide 36787 in intron 16) in HT (n = 152) and normotensive (NT, n = 193) groups having inherently high biological power (>80%) due to our inclusion only of subjects whose parents had the same BP status as themselves. The SNPs were in linkage disequilibrium (D' = 54% to 100%, P =.05 to 0.0001). Because ACE2 is on the X chromosome, data for each sex were analyzed separately. Minor allele frequencies in HT versus NT were as follows: for the intron 1 variant 0.21 versus 0.17 in female subjects (P =.31) and 0.25 versus 0.29 in male subjects (P =.60); intron 3 variant 0.22 versus 0.18 in female subjects (P =.35) and 0.15 versus 0.20 in male subjects (P =.47); intron 11 variant 0.39 versus 0.46 in male subjects (P = 0.17) and 0.31 versus 0.30 in male subjects (P =.96); intron 16 variant 0.20 versus 0.19 in female subjects (P =.72) and 0.17 versus 0.17 in male subjects (P =.95). Haplotype analysis was also negative. These data provide little support for ACE2 in genetic predisposition to HT. PMID:15233982

  4. Desert Dust Layers Over Polluted Marine Boundary Layers: ACE-2 Measurements and ACE-Asia Plans

    NASA Technical Reports Server (NTRS)

    Russell, Philip B.; Schmid, B.; Livingston, J. M.; Redemann, J.; Bergstrom, R. W.; Condon, Estelle P. (Technical Monitor)

    2000-01-01

    Aerosols in ACE-Asia are expected to have some commonalties with those in ACE-2, along with important differences. Among the commonalities are occurrences of desert dust layers over polluted marine boundary layers. Differences include the nature of the dust (yellowish in the East Asia desert outflow, vs. reddish-brown in the Sahara Outflow measured in ACE-2) and the composition of boundary-layer aerosols (e.g., more absorbing, soot and organic aerosol in-the Asian plume, caused by coal and biomass burning, with limited controls). In this paper we present ACE-2 measurements and analyses as a guide to our plans for ACE-2 Asia. The measurements include: (1) Vertical profiles of aerosol optical depth and extinction (380-1558 nm), and of water vapor column and concentration, from the surface through the elevated desert dust, measured by the 14-channel Ames Airborne Tracking Sunphotometer (AATS-14); (2) Comparisons of airborne and shipborne sunphotometer optical depths to satellite-retrieved values, with and without desert dust; (3) Comparisons between airborne Sunphotometer optical depth and extinction spectra and those derived from coincident airborne in situ measurements of aerosol size distribution, scattering and absorption; (4) Comparisons between size distributions measured in situ and retrieved from sunphotometer optical depth spectra; (5) Comparisons between aerosol single scattering albedo values obtained by several techniques, using various combinations of measurements of backscatter, extinction, size distribution, scattering, absorption, and radiative flux. We show how analyses of these data can be used to address questions important to ACE-Asia, such as: (1) How do dust and other absorbing aerosols affect the accuracy of satellite optical depth retrievals? How important are asphericity effects? (2) How important are supermicron dust and seasalt aerosols to overall aerosol optical depth and radiative forcing? How well are these aerosols sampled by aircraft

  5. Shipboard Sunphotometer Measurements of Aerosol Optical Depth Spectra and Columnar Water Vapor During ACE-2 and Comparison with Selected Land, Ship, Aircraft, and Satellite Measurements

    NASA Technical Reports Server (NTRS)

    Livingston, John M.; Kapustin, Vladimir N.; Schmid, Beat; Russell, Philip B.; Quinn, Patricia K.; Bates, Timothy S.; Durkee, Philip A.; Smith, Peter J.; Freudenthaler, Volker; Wiegner, Matthias; Covert, Dave S.; Gasso, Santiago; Hegg, Dean; Collins, Donald R.; Flagan, Richard C.; Seinfeld, John H.; Vitale, Vito; Tomasi, Claudio

    2000-01-01

    Analyses of aerosol optical depth (AOD) and colurnmn water vapor (CWV) measurements acquired with NASA Ames Research Center's 6-channel Airborne Tracking Sunphotometer (AATS-6) operated aboard the R/V Professor Vodyanitskiy during the 2nd Aerosol Characterization Experiment (ACE-2) are discussed. Data are compared with various in situ and remote measurements for selected cases. The focus is on 10 July, when the Pelican airplane flew within 70 km of the ship near the time of a NOAA-14/AVHRR satellite overpass and AOD measurements with the 14-channel Ames Airborne Tracking Sunphotometer (AATS-14) above the marine boundary layer (MBL) permitted calculation of AOD within the MBL from the AATS-6 measurements. A detailed column closure test is performed for MBL AOD on 10 July by comparing the AATS-6 MBL AODs with corresponding values calculated by combining shipboard particle size distribution measurements with models of hygroscopic growth and radiosonde humidity profiles (plus assumptions on the vertical profile of the dry particle size distribution and composition). Large differences (30-80% in the mid-visible) between measured and reconstructed AODs are obtained, in large part because of the high sensitivity of the closure methodology to hygroscopic growth models, which vary considerably and have not been validated over the necessary range of particle size/composition distributions. The wavelength dependence of AATS-6 AODs is compared with the corresponding dependence of aerosol extinction calculated from shipboard measurements of aerosol size distribution and of total scattering mearured by a shipboard integrating nephelometer for several days. Results are highly variable, illustrating further the great difficulty of deriving column values from point measurements. AATS-6 CWV values are shown to agree well with corresponding values derived from radiosonde measurements during 8 soundings on 7 days and also with values calculated from measurements taken on 10 July with

  6. Administration of 17β-estradiol to ovariectomized obese female mice reverses obesity-hypertension through an ACE2-dependent mechanism.

    PubMed

    Wang, Yu; Shoemaker, Robin; Thatcher, Sean E; Batifoulier-Yiannikouris, Frederique; English, Victoria L; Cassis, Lisa A

    2015-06-15

    We recently demonstrated that female mice are resistant to the development of obesity-induced hypertension through a sex hormone-dependent mechanism that involved adipose angiotensin-converting enzyme 2 (ACE2). In this study, we hypothesized that provision of 17β-estradiol (E2) to ovariectomized (OVX) high-fat (HF)-fed female hypertensive mice would reverse obesity-hypertension through an ACE2-dependent mechanism. Pilot studies defined dose-dependent effects of E2 in OVX female mice on serum E2 concentrations and uterine weights. An E2 dose of 36 μg/ml restored normal serum E2 concentrations and uterine weights. Therefore, HF-fed OVX female Ace2(+/+) and Ace2(-/-) mice were administered vehicle or E2 (36 μg/ml) for 16 wk. E2 administration significantly decreased body weights of HF-fed OVX female Ace2(+/+) and Ace2(-/-) mice of either genotype. At 15 wk, E2 administration decreased systolic blood pressure (SBP) of OVX HF-fed Ace2(+/+) but not Ace2(-/-) females during the light but not the dark cycle. E2-mediated reductions in SBP in Ace2(+/+) females were associated with significant elevations in adipose ACE2 mRNA abundance and activity and reduced plasma ANG II concentrations. In contrast to females, E2 administration had no effect on any parameter quantified in HF-fed male hypertensive mice. In 3T3-L1 adipocytes, E2 promoted ACE2 mRNA abundance through effects at estrogen receptor-α (ERα) and resulted in ERα-mediated binding at the ACE2 promoter. These results demonstrate that E2 administration to OVX females reduces obesity-induced elevations in SBP (light cycle) through an ACE2-dependent mechanism. Beneficial effects of E2 to decrease blood pressure in OVX obese females may result from stimulation of adipose ACE2. PMID:26078188

  7. Angiotensin I–Converting Enzyme Type 2 (ACE2) Gene Therapy Improves Glycemic Control in Diabetic Mice

    PubMed Central

    Bindom, Sharell M.; Hans, Chetan P.; Xia, Huijing; Boulares, A. Hamid; Lazartigues, Eric

    2010-01-01

    OBJECTIVE Several clinical studies have shown the benefits of renin-angiotensin system (RAS) blockade in the development of diabetes, and a local RAS has been identified in pancreatic islets. Angiotensin I–converting enzyme (ACE)2, a new component of the RAS, has been identified in the pancreas, but its role in β-cell function remains unknown. Using 8- and 16-week-old obese db/db mice, we examined the ability of ACE2 to alter pancreatic β-cell function and thereby modulate hyperglycemia. RESEARCH DESIGN AND METHODS Both db/db and nondiabetic lean control (db/m) mice were infected with an adenovirus expressing human ACE2 (Ad-hACE2-eGFP) or the control virus (Ad-eGFP) via injection into the pancreas. Glycemia and β-cell function were assessed 1 week later at the peak of viral expression. RESULTS In 8-week-old db/db mice, Ad-hACE2-eGFP significantly improved fasting glycemia, enhanced intraperitoneal glucose tolerance, increased islet insulin content and β-cell proliferation, and reduced β-cell apoptosis compared with Ad-eGFP. ACE2 overexpression had no effect on insulin sensitivity in comparison with Ad-eGFP treatment in diabetic mice. Angiotensin-(1–7) receptor blockade by d-Ala7–Ang-(1-7) prevented the ACE2-mediated improvements in intraperitoneal glucose tolerance, glycemia, and islet function and also impaired insulin sensitivity in both Ad-hACE2-eGFP– and Ad-eGFP–treated db/db mice. d-Ala7–Ang-(1-7) had no effect on db/m mice. In 16-week-old diabetic mice, Ad-hACE2-eGFP treatment improved fasting blood glucose but had no effect on any of the other parameters. CONCLUSIONS These findings identify ACE2 as a novel target for the prevention of β-cell dysfunction and apoptosis occurring in type 2 diabetes. PMID:20660625

  8. Representation of global inland water heights within the forthcoming ACE2 GDEM

    NASA Astrophysics Data System (ADS)

    Benveniste, Jerome; Smith, Richard G.; Berry, Philippa A. M.

    The SRTM dataset provides the first near-global elevation model of the earth's continental land surface at 3" resolution. This unique dataset is currently used for a range of applications. However, there are a number of known quirks and omissions in these data, for example voids over large inland water extents and errors in height attributed to river locations, for example where surrounding tree canopy has been measured. Accordingly, an enhanced full global elevation dataset, ACE2, is currently being developed as an ESA initiative, a follow-on to the highly successful ACE GDEM. This development is utilising over 67 million height measurements (derived from multi-mission satellite radar altimetry using an expert system approach) to validate, error correct and augment the SRTM dataset to derive a full global GDEM at 3 arc second resolution. This paper presents results obtained from a global investigation into the representation of inland water heights within current GDEMs, and demonstrates the potential to include not only accurate mean heights for inland water targets, but also to characterise the temporal variation utilising decadal timeseries obtained from multimission satellite radar altimetry. The first full release of the ACE2 GDEM is scheduled for later this year.

  9. Communications Link Characterization Experiment (CLCE)

    NASA Technical Reports Server (NTRS)

    1977-01-01

    Results of the data which were acquired, reduced, and analyzed from the Communications Link Characterization Experiment (CLCE) while utilizing the CTS satellite are presented. The test data obtained from the stations consisted of the results of various TV tests, attenuation and rain rate data. An additional meteorological parameter was measured and it consists of the back scatter returns of the multifrequency weather radar.

  10. Activation of angiotensin-converting enzyme 2 (ACE2) attenuates allergic airway inflammation in rat asthma model.

    PubMed

    Dhawale, Vaibhav Shrirang; Amara, Venkateswara Rao; Karpe, Pinakin Arun; Malek, Vajir; Patel, Deep; Tikoo, Kulbhushan

    2016-09-01

    Angiotensin-I converting enzyme (ACE) is positively correlated to asthma, chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS) and is highly expressed in lungs. ACE2, the counteracting enzyme of ACE, was proven to be protective in pulmonary, cardiovascular diseases. In the present study we checked the effect of ACE2 activation in animal model of asthma. Asthma was induced in male wistar rats by sensitization and challenge with ovalbumin and then treated with ACE2 activator, diminazene aceturate (DIZE) for 2weeks. 48h after last allergen challenge, animals were anesthetized, blood, BALF, femoral bone marrow lavage were collected for leucocyte count; trachea for measuring airway responsiveness to carbachol; lungs and heart were isolated for histological studies and western blotting. In our animal model, the characteristic features of asthma such as altered airway responsiveness to carbachol, eosinophilia and neutrophilia were observed. Western blotting revealed the increased pulmonary expression of ACE1, IL-1β, IL-4, NF-κB, BCL2, p-AKT, p-p38 and decreased expression of ACE2 and IκB. DIZE treatment prevented these alterations. Intraalveolar interstitial thickening, inflammatory cell infiltration, interstitial fibrosis, oxidative stress and right ventricular hypertrophy in asthma control animals were also reversed by DIZE treatment. Activation of ACE2 by DIZE conferred protection against asthma as evident from biochemical, functional, histological and molecular parameters. To the best of our knowledge, we report for the first time that activation of ACE2 by DIZE prevents asthma progression by altering AKT, p38, NF-κB and other inflammatory markers. PMID:27343405

  11. Alternative Roles of STAT3 and MAPK Signaling Pathways in the MMPs Activation and Progression of Lung Injury Induced by Cigarette Smoke Exposure in ACE2 Knockout Mice.

    PubMed

    Hung, Yi-Han; Hsieh, Wen-Yeh; Hsieh, Jih-Sheng; Liu, Fon-Chang; Tsai, Chin-Hung; Lu, Li-Che; Huang, Chen-Yi; Wu, Chien-Liang; Lin, Chih-Sheng

    2016-01-01

    Inflammation-mediated abnormalities in the renin-angiotensin system (RAS) and expression of matrix metalloproteinases (MMPs) are implicated in the pathogenesis of lung injury. Angiotensin converting enzyme II (ACE2), an angiotensin converting enzyme (ACE) homologue that displays antagonist effects on ACE/angiotensin II (Ang II) axis, could also play a protective role against lung diseases. However, the relationship between ACE2 and MMPs activation in lung injury is still largely unclear. The purpose of this study is to investigate whether MMPs activity could be affected by ACE2 and which ACE2 derived signaling pathways could be also involved via using a mouse model with lung injury induced by cigarette smoke (CS) exposure for 1 to 3 weeks. Wild-type (WT; C57BL/6) and ACE2 KO mice (ACE2(-/-)) were utilized to study CS-induced lung injury. Increases in the resting respiratory rate (RRR), pulmonary immunokines, leukocyte infiltration and bronchial hyperplasia were observed in the CS-exposed mice. Compared to WT mice, more serious physiopathological changes were found in ACE2(-/-) mice in the first week of CS exposure. CS exposure increased pulmonary ACE and ACE2 activities in WT mice, and significantly increased ACE in ACE2(-/-) mice. Furthermore, the activity of pulmonary MMPs was decreased in CS-exposed WT mice, whereas this activity was increased in ACE2(-/-) mice. CS exposure increased the pulmonary p-p38, p-JNK and p-ERK1/2 level in all mice. In ACE2(-/-) mice, a significant increase p-STAT3 signaling was detected; however, no effect was observed on the p-STAT3 level in WT mice. Our results support the hypothesis that ACE2 deficiency influences MMPs activation and STAT3 phosphorylation signaling to promote more pulmonary inflammation in the development of lung injury. PMID:27019629

  12. Alternative Roles of STAT3 and MAPK Signaling Pathways in the MMPs Activation and Progression of Lung Injury Induced by Cigarette Smoke Exposure in ACE2 Knockout Mice

    PubMed Central

    Hung, Yi-Han; Hsieh, Wen-Yeh; Hsieh, Jih-Sheng; Liu, Fon-Chang; Tsai, Chin-Hung; Lu, Li-Che; Huang, Chen-Yi; Wu, Chien-Liang; Lin, Chih-Sheng

    2016-01-01

    Inflammation-mediated abnormalities in the renin-angiotensin system (RAS) and expression of matrix metalloproteinases (MMPs) are implicated in the pathogenesis of lung injury. Angiotensin converting enzyme II (ACE2), an angiotensin converting enzyme (ACE) homologue that displays antagonist effects on ACE/angiotensin II (Ang II) axis, could also play a protective role against lung diseases. However, the relationship between ACE2 and MMPs activation in lung injury is still largely unclear. The purpose of this study is to investigate whether MMPs activity could be affected by ACE2 and which ACE2 derived signaling pathways could be also involved via using a mouse model with lung injury induced by cigarette smoke (CS) exposure for 1 to 3 weeks. Wild-type (WT; C57BL/6) and ACE2 KO mice (ACE2-/-) were utilized to study CS-induced lung injury. Increases in the resting respiratory rate (RRR), pulmonary immunokines, leukocyte infiltration and bronchial hyperplasia were observed in the CS-exposed mice. Compared to WT mice, more serious physiopathological changes were found in ACE2-/- mice in the first week of CS exposure. CS exposure increased pulmonary ACE and ACE2 activities in WT mice, and significantly increased ACE in ACE2-/- mice. Furthermore, the activity of pulmonary MMPs was decreased in CS-exposed WT mice, whereas this activity was increased in ACE2-/- mice. CS exposure increased the pulmonary p-p38, p-JNK and p-ERK1/2 level in all mice. In ACE2-/- mice, a significant increase p-STAT3 signaling was detected; however, no effect was observed on the p-STAT3 level in WT mice. Our results support the hypothesis that ACE2 deficiency influences MMPs activation and STAT3 phosphorylation signaling to promote more pulmonary inflammation in the development of lung injury. PMID:27019629

  13. Collectrin and ACE2 in renal and intestinal amino acid transport.

    PubMed

    Singer, Dustin; Camargo, Simone M R

    2011-01-01

    Neutral amino acid transporters of the SLC6 family are expressed at the apical membrane of kidney and/or small intestine, where they (re-)absorb amino acids into the body. In this review we present the results concerning the dependence of their apical expression with their association to partner proteins. We will in particular focus on the situation of B0AT1 and B0AT3, that associate with members of the renin-angiotensin system (RAS), namely Tmem27 and angiotensin-converting enzyme 2 (ACE2), in a tissue specific manner. The role of this association in relation to the formation of a functional unit related to Na+ or amino acid transport will be assessed. We will conclude with some remarks concerning the relevance of this association to Hartnup disorder, where some mutations have been shown to differentially interact with the partner proteins. PMID:21814048

  14. Clear-Sky Closure Studies of Lower Tropospheric Aerosol and Water Vapor During ACE-2 Using Airborne Sunphotometer, Airborne In-Situ, Space-Borne, and Ground-Based Measurements

    NASA Technical Reports Server (NTRS)

    Schmid, Beat; Livingston, John M.; Russell, Philip B.; Durkee, Philip A.; Jonsson, Haflidi H.; Collins, Donald R.; Flagan, Richard C.; Seinfeld, John H.; Gasso, Santiago; Hegg, Dean A.; Oestroem, Elisabeth; Voss, Kenneth J.; Gordon, Howard R.; Formenti, Paolo; Andreae, Meinrat O.

    2000-01-01

    We report on clear-sky column closure experiments (CLEARCOLUMN) performed in the Canary Islands during the second Aerosol Characterization Experiment (ACE-2) in June/July 1997. We present CLEARCOLUMN results obtained by combining airborne sunphotometer and in-situ (optical particle counter, nephelometer, and absorption photometer) measurements taken aboard the Pelican aircraft, space-borne NOAA/AVHRR data and ground-based lidar and sunphotometer measurements. During both days discussed here, vertical profiles flown in cloud-free air masses revealed 3 distinctly different layers: a marine boundary layer (MBL) with varying pollution levels, an elevated dust layer, and a very clean layer between the MBL and the dust layer. A key result of this study is the achievement of closure between extinction or layer aerosol optical depth (AOD) computed from continuous in-situ aerosol size-distributions and composition and those measured with the airborne sunphotometer. In the dust, the agreement in layer AOD (lambda = 380-1060 nm) is 3-8%. In the MBL there is a tendency for the in-situ results to be slightly lower than the sunphotometer measurements (10-17% at lambda = 525 nm), but these differences are within the combined error bars of the measurements and computations.

  15. Clear-Sky Closure Studies of Tropospheric Aerosol and Water Vapor During ACE-2 Using Airborne Sunphotometer, Airborne In-Situ, Space-Borne, and Ground-Based Measurements

    NASA Technical Reports Server (NTRS)

    Schmid, Beat; Collins, Donald R.; Gasso, Santiago; Oestroem, Elisabeth; Powell, Donna M.; Welton, Ellsworth J.; Durkee, Philip A.; Livingston, John M.; Russell, Philip B.; Flagan, Richard C.; Seinfeld, John H.; Hegg, Dean A.; Noone, Kevin J.; Voss, Kenneth J.; Gordon, Howard R.; Reagan, John A.; Spinhirne, James D.

    2000-01-01

    We report on clear-sky column closure experiments (CLEARCOLUMN) performed in the Canary Islands during the second Aerosol Characterization Experiment (ACE-2) in June/July 1997. We present CLEARCOLUMN results obtained by combining airborne sunphotometer and in-situ (a differential mobility analyzer, three optical particle counters, three nephelometers, and one absorption photometer) measurements taken aboard the Pelican aircraft, space-borne NOAA/AVHRR data and ground-based lidars. A wide range of aerosol types was encountered throughout the ACE-2 area, including background Atlantic marine, European pollution-derived, and (although less frequently than expected) African mineral dust. During the two days discussed here, vertical profiles flown in cloud free air masses revealed three distinctly different layers: a marine boundary layer (MBL) with varying pollution levels, an elevated dust layer, and a very clean layer between the MBL and the dust layer. Based on size-resolved composition information we have established an aerosol model that allows us to compute optical properties of the ambient aerosol using the optical particle counter results. In the dust, the agreement in layer AOD (lambda=380-1060 nm) is 3-8%. In the MBL there is a tendency for the in-situ results to be slightly lower than the sunphotometer measurements (10-17% at lambda=525 nm), but these differences are within the combined error bars of the measurements and computations. Aerosol size-distribudon closure based on in-situ size distributions and inverted sunphotometer extinction spectra has been achieved in the MBL (total surface area and volume agree within 0.2, and 7%, respectively) but not in the dust layer. The fact that the three nephelometers operated at three different relative humidities (RH) allowed to parameterize hygroscopic growth and to therefore estimate optical properties at ambient RH. The parameters derived for different aerosol types are themselves useful for the aerosol modeling

  16. ACE inhibition, ACE2 and angiotensin-(1-7) axis in kidney and cardiac inflammation and fibrosis.

    PubMed

    Simões E Silva, Ana Cristina; Teixeira, Mauro Martins

    2016-05-01

    The Renin Angiotensin System (RAS) is a pivotal physiological regulator of heart and kidney homeostasis, but also plays an important role in the pathophysiology of heart and kidney diseases. Recently, new components of the RAS have been discovered, including angiotensin converting enzyme 2 (ACE2), Angiotensin(Ang)-(1-7), Mas receptor, Ang-(1-9) and Alamandine. These new components of RAS are formed by the hydrolysis of Ang I and Ang II and, in general, counteract the effects of Ang II. In experimental models of heart and renal diseases, Ang-(1-7), Ang-(1-9) and Alamandine produced vasodilation, inhibition of cell growth, anti-thrombotic, anti-inflammatory and anti-fibrotic effects. Recent pharmacological strategies have been proposed to potentiate the effects or to enhance the formation of Ang-(1-7) and Ang-(1-9), including ACE2 activators, Ang-(1-7) in hydroxypropyl β-cyclodextrin, cyclized form of Ang-(1-7) and nonpeptide synthetic Mas receptor agonists. Here, we review the role and effects of ACE2, ACE2 activators, Ang-(1-7) and synthetic Mas receptor agonists in the control of inflammation and fibrosis in cardiovascular and renal diseases and as counter-regulators of the ACE-Ang II-AT1 axis. We briefly comment on the therapeutic potential of the novel members of RAS, Ang-(1-9) and alamandine, and the interactions between classical RAS inhibitors and new players in heart and kidney diseases. PMID:26995300

  17. Structural determinants for binding to angiotensin converting enzyme 2 (ACE2) and angiotensin receptors 1 and 2

    PubMed Central

    Clayton, Daniel; Hanchapola, Iresha; Thomas, Walter G.; Widdop, Robert E.; Smith, Alexander I.; Perlmutter, Patrick; Aguilar, Marie-Isabel

    2015-01-01

    Angiotensin converting enzyme 2 (ACE2) is a zinc carboxypeptidase involved in the renin–angiotensin system (RAS) and inactivates the potent vasopressive peptide angiotensin II (Ang II) by removing the C-terminal phenylalanine residue to yield Ang1–7. This conversion inactivates the vasoconstrictive action of Ang II and yields a peptide that acts as a vasodilatory molecule at the Mas receptor and potentially other receptors. Given the growing complexity of RAS and level of cross-talk between ligands and their corresponding enzymes and receptors, the design of molecules with selectivity for the major RAS binding partners to control cardiovascular tone is an on-going challenge. In previous studies we used single β-amino acid substitutions to modulate the structure of Ang II and its selectivity for ACE2, AT1R, and angiotensin type 2 (AT2R) receptor. We showed that modification at the C-terminus of Ang II generally resulted in more pronounced changes to secondary structure and ligand binding, and here, we further explore this region for the potential to modulate ligand specificity. In this study, (1) a library of 47 peptides derived from the C-terminal tetrapeptide sequence (-IHPF) of Ang II was synthesized and assessed for ACE2 binding, (2) the terminal group requirements for high affinity ACE2 binding were explored by and N- and C-terminal modification, (3) high affinity ACE2 binding chimeric AngII analogs were then synthesized and assessed, (4) the structure of the full-length Ang II analogs were assessed by circular dichroism, and (5) the Ang II analogs were assessed for AT1R/AT2R selectivity by cell-based assays. Studies on the C-terminus of Ang II demonstrated varied specificity at different residue positions for ACE2 binding and four Ang II chimeric peptides were identified as selective ligands for the AT2 receptor. Overall, these results provide insight into the residue and structural requirements for ACE2 binding and angiotensin receptor selectivity. PMID

  18. Antenatal maternal low protein diet: ACE-2 in the mouse lung and sexually dimorphic programming of hypertension.

    PubMed

    Goyal, Ravi; Van-Wickle, Jonathan; Goyal, Dipali; Longo, Lawrence D

    2015-01-01

    Elevated blood pressure is an important global health problem, and in-utero under-nutrition may be an important factor in the pathogenesis of hypertension. In the present study, we tested the hypothesis that antenatal maternal low protein diet (MLPD) leads to sexually dimorphic developmental programming of the components of the pulmonary renin-angiotensin system. This may be important in the antenatal MLPD-associated development of hypertension. In pregnant mice, we administered normal (control) and isocaloric 50% protein restricted diet, commencing one week before mating and continuing until delivery of the pups. From the 18th to 24th week postnatal, we measured blood pressure in the offspring by use of a non-invasive tail-cuff method. In the same mice, we examined the mRNA and protein expression of the key components of the pulmonary renin-angiotensin system. Also, we examined microRNA complementary to angiotensin converting enzymes (ACE) 2 in the offspring lungs. Our results demonstrate that as a consequence of antenatal MLPD: 1) pup birthweight was significantly reduced in both sexes. 2) female offspring developed hypertension, but males did not. 3) In female offspring, ACE-2 protein expression was significantly reduced without any change in the mRNA levels. 4) miRNA 429, which has a binding site on ACE-2 - 3' UTR was significantly upregulated in the female antenatal MLPD offspring. 5) In males, ACE-2 mRNA and protein expression were unaltered. We conclude that in the mouse, antenatal MLPD-induced reduction of ACE-2 in the female offspring lung may be an important mechanisms in sexually dimorphic programming of hypertension. PMID:25971747

  19. Administration of 17β-estradiol to ovariectomized obese female mice reverses obesity-hypertension through an ACE2-dependent mechanism

    PubMed Central

    Wang, Yu; Shoemaker, Robin; Thatcher, Sean E.; Batifoulier-Yiannikouris, Frederique; English, Victoria L.

    2015-01-01

    We recently demonstrated that female mice are resistant to the development of obesity-induced hypertension through a sex hormone-dependent mechanism that involved adipose angiotensin-converting enzyme 2 (ACE2). In this study, we hypothesized that provision of 17β-estradiol (E2) to ovariectomized (OVX) high-fat (HF)-fed female hypertensive mice would reverse obesity-hypertension through an ACE2-dependent mechanism. Pilot studies defined dose-dependent effects of E2 in OVX female mice on serum E2 concentrations and uterine weights. An E2 dose of 36 μg/ml restored normal serum E2 concentrations and uterine weights. Therefore, HF-fed OVX female Ace2+/+ and Ace2−/− mice were administered vehicle or E2 (36 μg/ml) for 16 wk. E2 administration significantly decreased body weights of HF-fed OVX female Ace2+/+ and Ace2−/− mice of either genotype. At 15 wk, E2 administration decreased systolic blood pressure (SBP) of OVX HF-fed Ace2+/+ but not Ace2−/− females during the light but not the dark cycle. E2-mediated reductions in SBP in Ace2+/+ females were associated with significant elevations in adipose ACE2 mRNA abundance and activity and reduced plasma ANG II concentrations. In contrast to females, E2 administration had no effect on any parameter quantified in HF-fed male hypertensive mice. In 3T3-L1 adipocytes, E2 promoted ACE2 mRNA abundance through effects at estrogen receptor-α (ERα) and resulted in ERα-mediated binding at the ACE2 promoter. These results demonstrate that E2 administration to OVX females reduces obesity-induced elevations in SBP (light cycle) through an ACE2-dependent mechanism. Beneficial effects of E2 to decrease blood pressure in OVX obese females may result from stimulation of adipose ACE2. PMID:26078188

  20. Collectrin, a homologue of ACE2, its transcriptional control and functional perspectives

    SciTech Connect

    Zhang Yanling Wada, Jun

    2007-11-09

    Collectrin is a type I membrane protein and shares significant homology with C-terminal domain of angiotensin-converting enzyme-2 (ACE2). However, collectrin lacks catalytic domain and it suggests the presence of uncharacterized physiological functions of collectrin. Collectrin is transcriptionally regulated by hepatocyte nuclear factor-{alpha} and -{beta} and is highly expressed on renal proximal tubules and collecting ducts as well as pancreatic {beta}-cells. Recent in vitro and in vivo studies demonstrated interesting physiological roles of collectrin related to insulin secretion, formation of primary cilia, renal cyst formation and amino acid transport. The common underlying molecular mechanism may be suggested by the evidence that collectrin binds to SNARE complex by interacting with snapin. Collectrin is involved in the process of vesicle transport and membrane fusion and thus it delivers insulin for exocytosis or various membrane proteins to apical plasmalemma and primary cilia. Collectrin may be the new therapeutic target for various pathological processes such as diabetes, polycystic kidney disease, hypertension and aminoaciduria.

  1. Role of Collectrin, an ACE2 Homologue, in Blood Pressure Homeostasis

    PubMed Central

    Chu, Pei-Lun; Le, Thu H.

    2014-01-01

    Collectrin (Tmem27) is a transmembrane glycoprotein that is highly expressed in the kidney and vascular endothelium. It is a homolog of the angiotensin converting enzyme 2 (ACE2) but harbors no catalytic domain. In the extravascular tissues of the kidney, collectrin is localized to the proximal tubule and collecting duct. Collectrin-deficient mice are featured with hypertension and exaggerated salt sensitivity. These phenotypes are associated with impaired uptake of the nitric oxide precursor L-arginine and the expression of its amino acid transporters, CAT-1 and y+LAT1, in endothelial cells. In addition, collectrin-deficient mice display decreased dimerization of nitric oxide synthase and decreased nitric oxide synthesis, but enhanced superoxide generation, suggesting that deletion of collectrin leads to a state of nitric oxide synthase uncoupling. These findings suggest collectrin plays a protective role against hypertension. The collectrin knockout mouse represents a unique model for hypertension research. Furthermore, collectrin may serve as a novel therapeutic target in the treatment of hypertension. PMID:25182162

  2. The transcription factor Ace2 and its paralog Swi5 regulate ethanol production during static fermentation through their targets Cts1 and Rps4a in Saccharomyces cerevisiae.

    PubMed

    Wu, Yao; Du, Jie; Xu, Guoqiang; Jiang, Linghuo

    2016-05-01

    Saccharomyces cerevisiae is the most widely used fermentation organism for ethanol production. However, the gene expression regulatory networks behind the ethanol fermentation are still not fully understood. Using a static fermentation model, we examined the ethanol yields on biomass of deletion mutants for 77 yeast genes encoding nonessential transcription factors, and found that deletion mutants for ACE2 and SWI5 showed dramatically increased ethanol yields. Overexpression of ACE2 or SWI5 in wild type cells reduced their ethanol yields. Furthermore, among the 34 target genes regulated by Ace2 and Swi5, deletion of CTS1,RPS4a,SIC1,EGT2,DSE2, or SCP160 led to increased ethanol yields, with the former two showing higher effects. Overexpression of CTS1 or RPS4a in both ace2/ace2 and swi5/swi5 mutants reduced their ethanol yields. In contrast, deletion of MCR1 or HO significantly decreased ethanol yields, with the former one showing the highest effect. Therefore, Ace2 and Swi5 are two negative regulators of ethanol yield during static fermentation of yeast cells, and both CTS1 and RPS4a are major effectors mediating these two transcription factors in regulating ethanol production. PMID:26975390

  3. ACE2 gene polymorphism and essential hypertension: an updated meta-analysis involving 11,051 subjects.

    PubMed

    Lu, Na; Yang, Yang; Wang, Yibo; Liu, Yan; Fu, Gang; Chen, Dongmei; Dai, Hui; Fan, Xiaohan; Hui, Rutai; Zheng, Yang

    2012-06-01

    The polymorphisms of angiotensin-converting enzyme 2 (ACE2) gene have been suggested to be linked to increase risk of essential hypertension in multiple populations. However, the results are still debatable. To assess the association between ACE2 G8970A genetic polymorphism and essential hypertension, we conducted a meta-analysis of case-control studies across different ethnicity. PubMed, Embase, CBM, Wanfang and VIP databases were searched, and a total of 11 separate studies in females and nine separate studies in males met the inclusion criteria. Because ACE2 is on the X chromosome, data for each sex were analyzed separately. The selected studies contained 7,251 (4,472 females/2,779 males) hypertensive patients and 3,800 (2,161 females/1,639 males) normotensive controls. A statistically significant association was observed between the G8970A gene polymorphism and essential hypertension risk in female hypertensive group in the recessive genetic model (AA vs. GG+GA: P = 0.03, OR = 1.15, 95% CI = 1.02-1.30, P(heterogeneity) = 0.40, I(2) = 5%, fixed-effects model). Although no association was shown between the frequency of the A allele and the genetic susceptibility to essential hypertension in all male patients (A Allele: P = 0.38, OR = 1.10, 95% CI = 0.89-1.38, P(heterogeneity) = 0.02, I(2) = 56%, random-effects model), we found that the relationship between carrier of A allele and the essential hypertension risk in Han-Chinese male patients subgroup (A Allele: P = 0.006, OR = 1.21, 95% CI = 1.06–1.38, P(heterogeneity) = 0.10, I(2) = 44%, fixed-effects model). The current meta-analysis provided solid evidence suggesting that ACE2 gene polymorphism G8790A was probably a genetic risk factor for essential hypertension across different ethnic populations in female subjects and in Han-Chinese male subjects. PMID:22297693

  4. Communications Link Characterization Experiment (CLCE), volume 3

    NASA Technical Reports Server (NTRS)

    1977-01-01

    The results of data which were acquired, reduced and analyzed as of June 1977, from the Communications Link Characterization Experiment while utilizing the Communications Link Characterization Experiment while utilizing the Communications Technology Satellite is presented. The long term yearly and worst month propagation data from both stations are presented for the defined yearly period and the test results of the Goddard Television Experiment are described. The data for the continuing path length analysis are updated. Progress on the continuing analysis of utilizing the weather radars for attenuation prediction is given and the concept of the weather radar ratio is presented.

  5. Angiotensin II regulates ACE and ACE2 in neurons through p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 signaling

    PubMed Central

    Xiao, Liang; Haack, Karla K. V.

    2013-01-01

    Brain ANG II plays an important role in modulating sympathetic function and homeostasis. The generation and degradation of ANG II are carried out, to a large extent, through the angiotensin-converting enzyme (ACE) and ACE2, respectively. In disease states, such as hypertension and chronic heart failure, central expression of ACE is upregulated and ACE2 is decreased in central sympathoregulatory neurons. In this study, we determined the expression of ACE and ACE2 in response to ANG II in a neuronal cell culture and the subsequent signaling mechanism(s) involved. A mouse catecholaminergic neuronal cell line (CATH.a) was treated with ANG II (30, 100, and 300 nM) for 24 h, and protein expression was determined by Western blot analysis. ANG II induced a significant dose-dependent increase in ACE and decrease in ACE2 mRNA and protein expression in CATH.a neurons. This effect was abolished by pretreatment of the cells with the p38 MAPK inhibitor SB-203580 (10 μM) 30 min before administration of ANG II or the ERK1/2 inhibitor U-0126 (10 μM). These data suggest that ANG II increases ACE and attenuates ACE2 expression in neurons via the ANG II type 1 receptor, p38 MAPK, and ERK1/2 signaling pathways. PMID:23535237

  6. [Effect of Astragali Radix in improving early renal damage in metabolic syndrome rats through ACE2/Mas pathway].

    PubMed

    Wang, Qiong-ying; Liang, Wei; Jiang, Cheng; Li, Ning-yin; Xu, Han; Yang, Mi-na; Lin, Xin; Yu, Heng; Chang, Peng; Yu, Jing

    2015-11-01

    To study the expression of angiotensin converting enzyme 2 (ACE2) and angiotensin (Ang) 1-7 specific receptor Mas protain in renal blood vessels of metabolic syndrome ( MS) rats and its anti-oxidative effect. A total of 80 male SD rats were divided into four groups: the normal control group (NC, the same volume of normal saline), the MS group (high fat diet), the MS + Astragali Radix group (MS + HQ, 6 g x kg(-1) x d(-1) in gavage) and the MS + Valsartan group (MS + XST, 30 mg x kg(-1) x d(-1) in gavage). After four weeks of intervention, their general indexes, biochemical indexes and blood pressure were measured; plasma and renal tissue Ang II, malondialdehyde (MDA) and superoxide demutase (SOD) levels were measured with radioimmunoassay. The protein expressions of Mas receptor, AT1R, ACE and ACE2 were detected by western blot analysis. According to the result, compared with the NC group, the MS group and the MS + HQ group showed significant increases in systolic and diastolic pressures, body weight, fasting glucose, fasting insulin, triglycerides, free fatty acid and Ang II level of MS rats (P < 0.05). The MS + XST group showed notable decreases in systolic and diastolic pressures than that of the MS group. The MS group showed significant increases in the SOD activity and NO level and decrease in the MDA level after being intervened with Astragali Radix. ACE and AT1R protein expressions in renal tissues of the MS group were higher than that in the NC group, but with lower ACE2 and -Mas receptor expressions (all P < 0.05). Compared with the MS group, the MS + HQ group showed significant increase in Mas receptor expression in renal tissues, whereas the MS + XST group showed notable decrease in AT1R (all P < 0.05). In conclusion, Astragali Radix can increase the Mas receptor expressions in renal tissues, decrease ACE expression and change local Ang II, MDA, NO and SOD in kidneys, so as to protect early damages in renal tissues. PMID:27071265

  7. Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry.

    PubMed

    Yu, Changhui; Tang, Wei; Wang, Yuhao; Shen, Qiang; Wang, Bin; Cai, Chunqing; Meng, Xiaojing; Zou, Fei

    2016-07-01

    The renin-angiotensin system (RAS) is an important component of the tumor microenvironment and plays a key role in promoting cancer cell proliferation, angiogenesis, metabolism, migration and invasion. Meanwhile, the arm of angiotensin-converting enzyme (ACE)2/angiotensin-(1-7) [Ang-(1-7)]/Mas axis in connection with RAS is associated with anti-proliferative, vasodilatory and anti-metastatic properties. Previous studies have shown that Ang-(1-7) reduces the proliferation of orthotopic human breast tumor growth by inhibiting cancer-associated fibroblasts. However, the role of ACE/Ang-(1-7)/Mas axis in the metastasis of breast cancer cells is still unknown. In the present study, we found that ACE2 protein level is negatively correlated with the metastatic ability of breast cancer cells and breast tumor grade. Upregulation of ACE2/Ang-(1-7)/Mas axis inhibits breast cancer cell migration and invasion in vivo and in vitro. Mechanistically, ACE2/Ang-(1-7)/Mas axis activation inhibits store-operated calcium entry (SOCE) and PAK1/NF-κB/Snail1 pathways, and induces E-cadherin expression. In summary, our results demonstrate that downregulation of ACE2/Ang-(1-7)/Mas axis stimulates breast cancer metastasis through the activation of SOCE and PAK1/NF-κB/Snail1 pathways. These results provide new mechanisms by which breast cancer develop metastasis and shed light on developing novel anti-metastasis therapeutics for metastatic breast cancer by modulating ACE2/Ang-(1-7)/Mas axis. PMID:27063099

  8. Functional and molecular evidence for expression of the renin angiotensin system and ADAM17-mediated ACE2 shedding in COS7 cells

    PubMed Central

    Grobe, Nadja; Di Fulvio, Mauricio; Kashkari, Nada; Chodavarapu, Harshita; Somineni, Hari K.; Singh, Richa

    2015-01-01

    The renin angiotensin system (RAS) plays a vital role in the regulation of the cardiovascular and renal functions. COS7 is a robust and easily transfectable cell line derived from the kidney of the African green monkey, Cercopithecus aethiops. The aims of this study were to 1) demonstrate the presence of an endogenous and functional RAS in COS7, and 2) investigate the role of a disintegrin and metalloproteinase-17 (ADAM17) in the ectodomain shedding of angiotensin converting enzyme-2 (ACE2). Reverse transcription coupled to gene-specific polymerase chain reaction demonstrated expression of ACE, ACE2, angiotensin II type 1 receptor (AT1R), and renin at the transcript levels in total RNA cell extracts. Western blot and immunohistochemistry identified ACE (60 kDa), ACE2 (75 kDa), AT1R (43 kDa), renin (41 kDa), and ADAM17 (130 kDa) in COS7. At the functional level, a sensitive and selective mass spectrometric approach detected endogenous renin, ACE, and ACE2 activities. ANG-(1–7) formation (m/z 899) from the natural substrate ANG II (m/z 1,046) was detected in lysates and media. COS7 cells stably expressing shRNA constructs directed against endogenous ADAM17 showed reduced ACE2 shedding into the media. This is the first study demonstrating endogenous expression of the RAS and ADAM17 in the widely used COS7 cell line and its utility to study ectodomain shedding of ACE2 mediated by ADAM17 in vitro. The transfectable nature of this cell line makes it an attractive cell model for studying the molecular, functional, and pharmacological properties of the renal RAS. PMID:25740155

  9. Lack of Association of ACE2 G8790A Gene Mutation with Essential Hypertension in the Chinese Population: A Meta-Analysis Involving 5260 Subjects

    PubMed Central

    Li, Yan-yan

    2012-01-01

    Background: The angiotensin converting enzyme 2 (ACE2) G8790A gene polymorphism has been associated with the susceptibility to essential hypertension (EH), but the results are disputable. Objective and Methods: To investigate the relationship between the ACE2 G8790A gene polymorphism and EH, eight separate studies with 5260 subjects were meta-analyzed. The pooled odds ratio (OR) and its corresponding 95% confidence interval (CI) were calculated by a random effect model. Results: In the ACE2 G8790A gene polymorphism and EH meta-analysis in a Chinese population, no significant association was found between the ACE2 G8790A gene polymorphism and EH (OR: 1.03, 95% CI: 0.87–1.21, P = 0.76). In the stratified analysis by gender, no significant risk was found among males (OR: 1.06, 95% CI: 0.82–1.36, P = 0.66) or females (OR: 0.98, 95% CI: 0.77–1.24, P = 0.85). Under a dominant model of inheritance in the female subgroup, the pooled OR for the GG/GA + AA value was 1.01 (95% CI: 0.82–1.25, P = 0.92). Under a recessive model of inheritance in the female subgroup, the pooled OR for the AA/AG + GG value was 0.93 (95% CI: 0.50–1.73, P = 0.83). Conclusion: The current meta-analysis suggested that the ACE2 G8790A gene polymorphism might not be related to the increased EH risk in the Chinese population. PMID:22988445

  10. Lethal Infection of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome Coronavirus▿

    PubMed Central

    McCray, Paul B.; Pewe, Lecia; Wohlford-Lenane, Christine; Hickey, Melissa; Manzel, Lori; Shi, Lei; Netland, Jason; Jia, Hong Peng; Halabi, Carmen; Sigmund, Curt D.; Meyerholz, David K.; Kirby, Patricia; Look, Dwight C.; Perlman, Stanley

    2007-01-01

    The severe acute respiratory syndrome (SARS), caused by a novel coronavirus (SARS-CoV), resulted in substantial morbidity, mortality, and economic losses during the 2003 epidemic. While SARS-CoV infection has not recurred to a significant extent since 2003, it still remains a potential threat. Understanding of SARS and development of therapeutic approaches have been hampered by the absence of an animal model that mimics the human disease and is reproducible. Here we show that transgenic mice that express the SARS-CoV receptor (human angiotensin-converting enzyme 2 [hACE2]) in airway and other epithelia develop a rapidly lethal infection after intranasal inoculation with a human strain of the virus. Infection begins in airway epithelia, with subsequent alveolar involvement and extrapulmonary virus spread to the brain. Infection results in macrophage and lymphocyte infiltration in the lungs and upregulation of proinflammatory cytokines and chemokines in both the lung and the brain. This model of lethal infection with SARS-CoV should be useful for studies of pathogenesis and for the development of antiviral therapies. PMID:17079315

  11. The POLARBEAR Experiment: Design and Characterization

    NASA Astrophysics Data System (ADS)

    Kermish, Zigmund David

    We present the design and characterization of the POLARBEAR experiment. POLARBEAR is a millimeter-wave polarimeter that will measure the Cosmic Microwave Background (CMB) polarization. It was designed to have both the sensitivity and angular resolution to detect the expected B-mode polarization due to gravitational lensing at small angular scales while still enabling a search for the degree scale B-mode polarization caused by inflationary gravitational waves. The instrument utilizes the Huan Tran Telescope (HTT), a 2.5-meter primary mirror telescope, coupled to a unique focal plane of 1,274 antenna-coupled transition-edge sensor (TES) detectors to achieve unprecedented sensitivity from angular scales of the experiment's 4 arcminute beam to several degrees. This dissertation focuses on the design, integration and characterization of the cryogenic receiver for the POLARBEAR instrument. The receiver cools the ˜20 cm focal plane to 0.25 Kelvin, with detector readout provided by a digital frequency-multiplexed SQUID system. The POLARBEAR receiver was been successfully deployed on the HTT for an engineering run in the Eastern Sierras of California and is currently deployed on Cerro Toco in the Atacama Dessert of Chile. We present results from lab tests done to characterize the instrument, from the engineering run and preliminary results from Chile.

  12. Experiments In Characterizing Vibrations Of A Structure

    NASA Technical Reports Server (NTRS)

    Yam, Yeung; Hadaegh, Fred Y.; Bayard, David S.

    1993-01-01

    Report discusses experiments conducted to test methods of identification of vibrational and coupled rotational/vibrational modes of flexible structure. Report one in series that chronicle development of integrated system of methods, sensors, actuators, analog and digital signal-processing equipment, and algorithms to suppress vibrations in large, flexible structure even when dynamics of structure partly unknown and/or changing. Two prior articles describing aspects of research, "Autonomous Frequency-Domain Indentification" (NPO-18099), and "Automated Characterization Of Vibrations Of A Structure" (NPO-18141).

  13. Olmesartan Inhibits Cardiac Hypertrophy in Mice Overexpressing Renin Independently of Blood Pressure: Its Beneficial Effects on ACE2/Ang(1-7)/Mas Axis and NADPH Oxidase Expression.

    PubMed

    Tanno, Tomohiro; Tomita, Hirofumi; Narita, Ikuyo; Kinjo, Takahiko; Nishizaki, Kimitaka; Ichikawa, Hiroaki; Kimura, Yoshihiro; Tanaka, Makoto; Osanai, Tomohiro; Okumura, Ken

    2016-06-01

    Enhanced renin-angiotensin activity causes hypertension and cardiac hypertrophy. The angiotensin (Ang)-converting enzyme (ACE)2/Ang(1-7)/Mas axis pathway functions against Ang II type 1 receptor (AT1R) signaling. We investigated whether olmesartan (Olm), an AT1R blocker, inhibits cardiac hypertrophy independently of blood pressure, and evaluated the potential mechanisms. The 3- to 4-month-old male mice overexpressing renin in the liver (Ren-Tg) were given Olm (5 mg/kg/d) and hydralazine (Hyd) (3.5 mg/kg/d) orally for 2 months. Systolic blood pressure was higher in the Ren-Tg mice than in wild-type littermates. Olm and Hyd treatments lowered systolic blood pressure to the same degree. However, cardiac hypertrophy, evaluated by echocardiography, heart weight, cross-sectional area of cardiomyocytes, and gene expression, was inhibited by only Olm treatment, but not by Hyd. Olm treatment reversed decreased gene expressions of ACE2 and Mas receptor of Ren-Tg mice and inhibited enhanced NADPH oxidase (Nox)4 expression and reactive oxygen species, whereas Hyd treatment had no influence on them. These findings indicate that Olm treatment inhibits cardiac hypertrophy independently of blood pressure, not only through its original AT1R blockade but partly through enhancement of ACE2/Ang(1-7)/Mas axis and suppression of Nox4 expression. PMID:26886190

  14. Halogen Occultation Experiment (HALOE) optical filter characterization

    NASA Technical Reports Server (NTRS)

    Harvey, Gale A.

    1989-01-01

    The Halogen Occultation Experiment (HALOE) is a solar occultation experiment that will fly on the Upper Atmosphere Research Satellite to measure mixing ratio profiles of O3, H2O, NO2, NO, CH4, HCl, and HF. The inversion of the HALOE data will be critically dependent on a detailed knowledge of eight optical filters. A filter characterization program was undertaken to measure in-band transmissions, out-of-band transmissions, in-band transmission shifts with temperature, reflectivities, and age stability. Fourier Transform Infrared Spectrometers were used to perform measurements over the spectral interval 400/cm to 6300/cm (25 micrometers to 1.6 micrometers). Very high precision (0.1 percent T) in-band measurements and very high resolution (0.0001 percent T) out-of-band measurements have been made. The measurements revealed several conventional leaks at 0.01 percent transmission and greatly enhanced (1,000) leaks to the 2-element filters when placed in a Fabry-Perot cavity. Filter throughput changes by 5 percent for a 25 C change in filter temperature.

  15. Characterization of the Source Physics Experiment Site

    NASA Astrophysics Data System (ADS)

    Sussman, A. J.; Schultz-Fellenz, E. S.; Broome, S. T.; Townsend, M.; Abbott, R. E.; Snelson, C. M.; Cogbill, A. H.; Conklin, G.; Mitra, G.; Sabbeth, L.

    2012-12-01

    Designed to improve long-range treaty monitoring capabilities, the Source Physics Experiments, conducted at the Nevada National Security Site, also provide an opportunity to advance near-field monitoring and field-based investigations of suspected underground test locations. In particular, features associated with underground testing can be evaluated using Source Physics Experiment activities as analogs, linking on-site inspections with remote sensing technologies. Following a calibration shot (SPE 1), SPE 2 (10/2011) and SPE 3 (07/2012) were performed in the same emplacement hole with 1.0 ton of explosives at 150 ft depth. Because one of the goals of the Source Physics Experiments is to determine damage effects on seismic wave propagation and improve modeling capabilities, a key component in the predictive component and ultimate validation of the models is a full understanding of the intervening geology between the source and instrumented bore holes. Ground-based LIDAR and fracture mapping, mechanical properties determined via laboratory testing of rock core, discontinuity analysis and optical microscopy of the core rocks were performed prior to and following each experiment. In addition, gravity and magnetic data were collected between SPE 2 and 3. The source region of the explosions was also characterized using cross-borehole seismic tomography and vertical seismic profiling utilizing two sets of two boreholes within 40 meters of ground zero. The two sets of boreholes are co-linear with the explosives hole in two directions. Results of the LIDAR collects from both SPE 2 and 3 indicate a permanent ground displacement of up to several centimeters aligning along the projected surface traces of two faults observed in the core and fractures mapped at the surface. Laboratory testing and optical work show a difference in the characteristics of the rocks below and above 40 feet and within the fault zones.The estimated near-surface densities from the gravity survey show

  16. Deficiency of ACE2 in Bone-Marrow-Derived Cells Increases Expression of TNF-α in Adipose Stromal Cells and Augments Glucose Intolerance in Obese C57BL/6 Mice.

    PubMed

    Thatcher, Sean E; Gupte, Manisha; Hatch, Nicholas; Cassis, Lisa A

    2012-01-01

    Deficiency of ACE2 in macrophages has been suggested to promote the development of an inflammatory M1 macrophage phenotype. We evaluated effects of ACE2 deficiency in bone-marrow-derived stem cells on adipose inflammation and glucose tolerance in C57BL/6 mice fed a high fat (HF) diet. ACE2 activity was increased in the stromal vascular fraction (SVF) isolated from visceral, but not subcutaneous adipose tissue of HF-fed mice. Deficiency of ACE2 in bone marrow cells significantly increased mRNA abundance of F4/80 and TNF-α in the SVF isolated from visceral adipose tissue of HF-fed chimeric mice, supporting increased presence of inflammatory macrophages in adipose tissue. Moreover, deficiency of ACE2 in bone marrow cells modestly augmented glucose intolerance in HF-fed chimeric mice and increased blood levels of glycosylated hemoglobin. In summary, ACE2 deficiency in bone marrow cells promotes inflammation in adipose tissue and augments obesity-induced glucose intolerance. PMID:22518292

  17. Chronic Treatment with Ang-(1-7) Reverses Abnormal Reactivity in the Corpus Cavernosum and Normalizes Diabetes-Induced Changes in the Protein Levels of ACE, ACE2, ROCK1, ROCK2 and Omega-Hydroxylase in a Rat Model of Type 1 Diabetes

    PubMed Central

    Yousif, Mariam H. M.; Makki, Batoul; El-Hashim, Ahmed Z.; Benter, Ibrahim F.

    2014-01-01

    Angiotensin-(1-7) [Ang-(1-7)] may have beneficial effects in diabetes mellitus-induced erectile dysfunction (DMIED) but its molecular actions in the diabetic corpus cavernosum (CC) are not known. We characterized the effects of diabetes and/or chronic in vivo administration of Ang-(1-7) on vascular reactivity in the rat corpus cavernosum (CC) and on protein expression levels of potential downstream effectors of the renin-angiotensin-aldosterone system (RAAS) such as angiotensin-converting enzyme (ACE), ACE2, Rho kinases 1 and 2 (ROCK1 and ROCK2), and omega-hydroxylase, the cytochrome-P450 enzyme that metabolizes arachidonic acid to form the vasoconstrictor, 20-hydroxyeicosatetraenoic acid. Streptozotocin-treated rats were chronicically administered Ang-(1-7) with or without A779, a Mas receptor antagonist, during weeks 4 to 6 of diabetes. Ang-(1-7) reversed diabetes-induced abnormal reactivity to vasoactive agents (endothelin-1, phenylepherine, and carbachol) in the CC without correcting hyperglycemia. Six weeks of diabetes led to elevated ACE, ROCK1, ROCK 2, and omega-hydroxylase and a concomitant decrease in ACE2 protein expression levels that were normalized by Ang-(1-7) treatment but not upon coadministration of A779. These data are supportive of the notion that the beneficial effects of Ang-(1-7) in DMIED involve counterregulation of diabetes-induced changes in ACE, ACE2, Rho kinases, and omega-hydroxylase proteins in the diabetic CC via a Mas receptor-dependent mechanism. PMID:25309930

  18. Evaluation of the differences between the SRTM and satellite radar altimetry height measurements and the approach taken for the ACE2 GDEM in areas of large disagreement.

    PubMed

    Smith, Richard Gavin; Berry, Philippa A M

    2011-06-01

    The new ACE2 (Altimeter Corrected Elevations 2) Global Digital Elevation Model (GDEM) which has recently been released aims to provide the most accurate GDEM to date. ACE2 was created by synergistically merging the SRTM and altimetry datasets. The comprehensive comparison carried out between the two datasets yielded a myriad of information, with the areas of disagreement providing as much valuable information as the areas of agreement. Analysis of the comparison dataset revealed that certain topographic features displayed consistent differences between the two datasets. The largest differences globally are present over the rainforests, particularly the two largest, around the Amazon and the Congo. The differences range between 10 m and 40 m; these differences can be attributed to the height of the rainforest canopy, as the SRTM returned height values from somewhere within the uppermost reaches of the vegetation whereas the altimeter was able to penetrate through and return true ground heights. The second major class of terrain feature to demonstrate coherent differences are desert regions; here, different deserts present different characteristics. The final area of interest is that of Wetlands; these are areas of special significance because even a slight misrepresentation of the heights can have wide ranging effects in modelling wetland areas. These examples illustrate the valuable additional information content gleaned from the synergistic global combination of the two datasets. PMID:21509388

  19. MISSE Scattered Atomic Oxygen Characterization Experiment

    NASA Technical Reports Server (NTRS)

    Banks, Bruce A.; deGroh, Kim K.; Miller, Sharon K.

    2006-01-01

    An experiment designed to measure the atomic oxygen (AO) erosion profile of scattered AO was exposed to Low Earth Orbital (LEO) AO for almost four years as part of the Materials International Space Station Experiment 1 and 2 (MISSE 1 and 2). The experiment was flown in MISSE Passive Experiment Carrier 2 (PEC 2), Tray 1, attached to the exterior of the International Space Station (ISS) Quest Airlock. The experiment consisted of an aperture disk lid of Kapton H (DuPont) polyimide coated on the space exposed surface with a thin AO durable silicon dioxide film. The aperture lid had a small hole in its center to allow AO to enter into a chamber and impact a base disk of aluminum. The AO that scattered from the aluminum base could react with the under side of the aperture lid which was coated sporadically with microscopic sodium chloride particles. Scattered AO erosion can occur to materials within a spacecraft that are protected from direct AO attack but because of apertures in the spacecraft the AO can attack the interior materials after scattering. The erosion of the underside of the Kapton lid was sufficient to be able to use profilometry to measure the height of the buttes that remained after washing off the salt particles. The erosion pattern indicated that peak flux of scattered AO occurred at and angle of approximately 45 from the incoming normal incidence on the aluminum base unlike the erosion pattern predicted for scattering based on Monte Carlo computational predictions for AO scattering from Kapton H polyimide. The effective erosion yield for the scattered AO was found to be a factor of 0.214 of that for direct impingement on Kapton H polyimide.

  20. Experiment to Characterize Tropical Cloud Systems

    SciTech Connect

    May, Peter T.; Mather, Jim H.; Jakob, Christian

    2005-08-02

    A major experiment to study tropical convective cloud systems and their impacts will take place around Darwin, Northern Australia in early 2006. The Tropical Warm Pool International Cloud Experiment (TWP-ICE) is a collaboration including the DOE ARM (Atmospheric Radiation Measurement) and ARM-UAV programs, NASA centers, the Australian Bureau of Meteorology, CSIRO, and universities in the USA, Australia, Japan, the UK, and Canada. TWP-ICE will be preceded in November/December 2004 by a collaborating European aircraft campaign involving the EU SCOUT-O3 and UK NERC ACTIVE projects. Detailed atmospheric measurements will be made in the Darwin area through the whole Austral summer, giving unprecedented coverage through the pre-monsoon and monsoon periods.

  1. Recent X-Ray Laser Characterization Experiments at LLNL

    SciTech Connect

    Smith, R F; Dunn, J; Nilsen, J; Fiedorowicz, H; Bartnik, A; Shlyaptsev, V N

    2002-02-26

    We report on a series of experiments, using the COMET picosecond facility, designed to characterize and develop different x-ray laser sources. This work encompasses collisional pumping of slab and gas puff targets.

  2. Hydrocarbon characterization experiments in fully turbulent fires.

    SciTech Connect

    Ricks, Allen; Blanchat, Thomas K.

    2007-05-01

    As the capabilities of numerical simulations increase, decision makers are increasingly relying upon simulations rather than experiments to assess risks across a wide variety of accident scenarios including fires. There are still, however, many aspects of fires that are either not well understood or are difficult to treat from first principles due to the computational expense. For a simulation to be truly predictive and to provide decision makers with information which can be reliably used for risk assessment the remaining physical processes must be studied and suitable models developed for the effects of the physics. The model for the fuel evaporation rate in a liquid fuel pool fire is significant because in well-ventilated fires the evaporation rate largely controls the total heat release rate from the fire. A set of experiments are outlined in this report which will provide data for the development and validation of models for the fuel regression rates in liquid hydrocarbon fuel fires. The experiments will be performed on fires in the fully turbulent scale range (> 1 m diameter) and with a number of hydrocarbon fuels ranging from lightly sooting to heavily sooting. The importance of spectral absorption in the liquid fuels and the vapor dome above the pool will be investigated and the total heat flux to the pool surface will be measured. The importance of convection within the liquid fuel will be assessed by restricting large scale liquid motion in some tests. These data sets will provide a sound, experimentally proven basis for assessing how much of the liquid fuel needs to be modeled to enable a predictive simulation of a fuel fire given the couplings between evaporation of fuel from the pool and the heat release from the fire which drives the evaporation.

  3. Attenuation of myocardial fibrosis with curcumin is mediated by modulating expression of angiotensin II AT1/AT2 receptors and ACE2 in rats

    PubMed Central

    Pang, Xue-Fen; Zhang, Li-Hui; Bai, Feng; Wang, Ning-Ping; Garner, Ron E; McKallip, Robert J; Zhao, Zhi-Qing

    2015-01-01

    Curcumin is known to improve cardiac function by balancing degradation and synthesis of collagens after myocardial infarction. This study tested the hypothesis that inhibition of myocardial fibrosis by curcumin is associated with modulating expression of angiotensin II (Ang II) receptors and angiotensin-converting enzyme 2 (ACE2). Male Sprague Dawley rats were subjected to Ang II infusion (500 ng/kg/min) using osmotic minipumps for 2 and 4 weeks, respectively, and curcumin (150 mg/kg/day) was fed by gastric gavage during Ang II infusion. Compared to the animals with Ang II infusion, curcumin significantly decreased the mean arterial blood pressure during the course of the observation. The protein level of the Ang II type 1 (AT1) receptor was reduced, and the Ang II type 2 (AT2) receptor was up-regulated, evidenced by an increased ratio of the AT2 receptor over the AT1 receptor in the curcumin group (1.2±0.02%) vs in the Ang II group (0.7±0.03%, P<0.05). These changes were coincident with less locally expressed AT1 receptor and enhanced AT2 receptor in the intracardiac vessels and intermyocardium. Along with these modulations, curcumin significantly decreased the populations of macrophages and alpha smooth muscle actin-expressing myofibroblasts, which were accompanied by reduced expression of transforming growth factor beta 1 and phosphorylated-Smad2/3. Collagen I synthesis was inhibited, and tissue fibrosis was attenuated, as demonstrated by less extensive collagen-rich fibrosis. Furthermore, curcumin increased protein level of ACE2 and enhanced its expression in the intermyocardium relative to the Ang II group. These results suggest that curcumin could be considered as an add-on therapeutic agent in the treatment of fibrosis-derived heart failure patient who is intolerant of ACE inhibitor therapy. PMID:26648693

  4. Comparison of Aerosol Single Scattering Albedos Derived by Diverse Techniques In Two North Atlantic Experiments

    NASA Technical Reports Server (NTRS)

    Russell, P. B.; Redemann, J.; Schmid, B.; Bergstrom, R. W.; Livingston, J. M.; McIntosh, D. M.; Ramirez, S. A.; Hartley, S.; Hobbs, P. V.; Quinn, P. K.

    2002-01-01

    Aerosol single scattering albedo omega (the ratio of scattering to extinction) is important in determining aerosol climatic effects, in explaining relationships between calculated and measured radiative fluxes, and in retrieving aerosol optical depths from satellite radiances. Recently, two experiments in the North Atlantic region, the Tropospheric Aerosol Radiative Forcing Observational Experiment (TARFOX) and the Second Aerosol Characterization Experiment (ACE-2), determined aerosol omega by a variety of techniques. The techniques included fitting of calculated to measured radiative fluxes; retrievals of omega from skylight radiances; best fits of complex refractive index to profiles of backscatter extinction, and size distribution; and in situ measurements of scattering and absorption at the surface and aloft. Both TARFOX and ACE-2 found a fairly wide range of values for omega at midvisable wavelengths approx. 550 nm, with omega(sub midvis) greater than or equal to 0.85 and less than or equal to 0.99 for the marine aerosol impacted by continental pollution. Frequency distributions of omega could usually be approximated by lognormals in omega(sub max) - omega, with some occurrence of bimodality, suggesting the influence of different aerosol sources or processing. In both TARFOX and ACE-2, closure tests between measured and calculated radiative fluxes yielded best-fit values of omega(sub midvis) 0.90 +/- 0.04 for the polluted boundary layer. Although these results have the virtue of describing the column aerosol unperturbed by sampling, they are subject to questions about representativeness and other uncertainties (e.g., thermal offsets, unknown gas absorption) The other techniques gave larger values for omega(sub midvis) for the polluted boundary layer, with a typical result of omega(sub midvis) = 0.95 +/- 0.04. Current uncertainties in omega are large in terms of climate effects More tests are needed of the consistency among different methods and of

  5. Characterization of metals melting discs Skylab experiment M551

    NASA Technical Reports Server (NTRS)

    Monroe, R. E.

    1974-01-01

    Characterizations of flight and ground based samples from the metals melting experiment are detailed. The characteristics were determined by nondestructive investigation, visual observation, metallographic examination, and post-test measurements. Comparisons of the flight and ground based discs showed that an electron beam heat source can be used in zero gravity for cutting, welding, or melting.

  6. Altitude Differentiated Aerosol Extinction Over Tenerife (North Atlantic Coast) During ACE-2 by Means of Ground and Airborne Photometry and Lidar Measurements

    NASA Technical Reports Server (NTRS)

    Formenti, P.; Elias, T.; Welton, J.; Diaz, J. P.; Exposito, F.; Schmid, B.; Powell, D.; Holben, B. N.; Smirnov, A.; Andreae, M. O.; Devaux, C.; Voss, K.; Lelieveld, J.; Livingston, J. M.; Russell, P. B.; Durkee, P. A.

    2000-01-01

    Retrievals of spectral aerosol optical depths (tau(sub a)) by means of sun photometers have been undertaken in Tenerife (28 deg 16' N, 16 deg 36' W) during ACE-2 (June-July 1997). Five ground-based sites were located at four different altitudes in the marine boundary layer and in the free troposphere, from 0 to 3570 m asl. The goal of the investigation was to provide estimates of the vertical aerosol extinction over the island, both under clean and turbid conditions. Inversion of spectral tau(sub a) allowed to retrieve size distributions, from which the single scattering albedo omega(sub 0) and the asymmetry factor g could be estimated as a function of altitude. These parameters were combined to calculate aerosol forcing in the column. Emphasis is put on episodes of increased turbidity, which were observed at different locations simultaneously, and attributed to outbreaks of mineral dust from North Africa. Differentiation of tau(sub a) as a function of altitude provided the vertical profile of the extinction coefficient sigma(sub e). For dust outbreaks, aerosol extinction is concentrated in two distinct layers above and below the strong subsidence inversion around 1200 m asl. Vertical profiles of tau(sub a) and sigma(sub e) are shown for July 8. In some occasions, vertical profiles are compared to LIDAR observations, performed both at sea level and in the low free troposphere, and to airborne measurements of aerosol optical depths.

  7. Characterization of Settled Atmospheric Dust by the DART Experiment

    NASA Technical Reports Server (NTRS)

    Landis, Geoffrey A.; Jenkins, Phillip P.; Baraona, Cosmo

    1999-01-01

    The DART ("Dust Accumulation and Removal Test") package is an experiment which will fly as part of the MIP experiment on the Mars-2001 Surveyor Lander. Dust deposition could be a significant problem for photovoltaic array operation for long duration emissions on the surface of Mars. Measurements made by Pathfinder showed 0.3% loss of solar array performance per day due to dust obscuration. The DART experiment is designed to quantify dust deposition from the Mars atmosphere, measure the properties of settled dust, measure the effect of dust deposition on the array performance, and test several methods of mitigating the effect of settled dust on a solar array. Although the purpose of DART (along with its sister experiment, MATE) is to gather information critical to the design of future power systems on the surface of Mars, the dust characterization instrumentation on DART will also provide significant scientific data on the properties of settled atmospheric dust.

  8. Test plan for FY-94 digface characterization field experiments

    SciTech Connect

    Josten, N.E.; Roybal, L.G.

    1994-08-01

    The digface characterization concept has been under development at the Idaho National Engineering Laboratory (INEL) since fiscal year (FY) 1992 through the support of the Buried Waste Integrated Demonstration Program. A digface characterization system conducts continuous subsurface characterization simultaneously with retrieval of hazardous and radioactive waste from buried waste sites. The system deploys multiple sensors at the retrieval operation digface and collects data that provide a basis for detecting, locating, and classifying buried materials and hazardous conditions before they are disturbed by the retrieval equipment. This test plan describes ongoing efforts to test the digface characterization concept at the INEL`s Cold Test Pit using a simplified prototype deployment apparatus and off-the-shelf sensors. FY-94 field experiments will explore problems in object detection and classification. Detection and classification of objects are fundamental to three of the four primary functions of digface characterization during overburden removal. This test plan establishes procedures for collecting and validating the digface characterization data sets. Analysis of these data will focus on testing and further developing analysis methods for object detection and classification during overburden removal.

  9. A Dust Characterization Experiment for Solar Cells Operating on Mars

    NASA Technical Reports Server (NTRS)

    Jenkins, Phillip; Landis, Geoffrey A.; Krasowski, Michael; Greer, Lawrence; Wilt, David; Baraona, Cosmo; Scheiman, David; Lekki, John

    2001-01-01

    During the Viking and Pathfinder missions to Mars, significant amounts of dust accumulated on the spacecrafts. In Pathfinder's case, the dust obscured the solar panels on the lander and the rover degrading their output current. The material adherence experiment aboard the Pathfinder rover quantified the rate of decrease in short circuit current at 0.28% per day. This rate is unacceptably high for long duration missions. In response, NASA has developed the Dust Accumulation and Removal Technology (DART) experiment. DART has three instruments for characterizing dust settling out of the atmosphere and tests two methods to keep dust from settling on solar cells.

  10. Characterization of Metals Melting Discs: Skylab Experiment M551

    NASA Technical Reports Server (NTRS)

    Monroe, R. E.

    1973-01-01

    Information developed to characterize flight and ground based samples from the metals melting experiment is detailed in this report. Included are the characteristics determined by nondestructive examination, visual observation, metallographic examination and posttest measurements. Comparisons of the flight and ground based discs showed that an electron beam heat source can be used successfully in zero gravity for cutting, welding, or melting. Few differences were observed that could be attributed to the absence of gravity in these operations.

  11. Material Analysis/Characterization for Ultra-Low Background Experiments

    NASA Astrophysics Data System (ADS)

    Jasinski, Ben

    For the discovery of rare event physics processes related to new physics beyond the Standard Model, the next generation of physics experiments requires to be built underground. For instance, the Large Underground Xenon experiment and MAJORANA DEMONSTRATOR, both located underground at the Sanford Underground Research Facility (SURF), require an ultra-low background rate for their respective discovery of Dark Matter and the Majorana particle. The next generation of detectors designated for these ultra-low background experiments require a complete understanding of the materials used in the experiment to achieve the detection sensitivity below the current limits. Experimental limits can be seen in ton scale detector designs where ultrapure materials are needed for detector performance, suppression of background radiation, and electronic noise. This thesis studies the three main aspects of ultra-low background experiments. First, an investigation of the mechanical design constraints for SURF underground physics experiments is performed. Second, a study into each detector's characterization and performance is described for the SURF experiments. Lastly, an optimization of data analysis algorithm for rare event physics is discussed. We describe the techniques used for the above three investigations in detail. First, the test of the mechanical design constraints is investigated using an adaptive sample holder capable of testing sample materials of gas, liquid or solid state. Second, the characterization of materials is studied with a design of a Capacitance-Voltage Measurement system. Lastly, the optimization of data analysis algorithm for small signals is performed by a detailed data analysis to verify isotopic enrichment obtained from a novel isotope separation method of molecules of CO2.

  12. Activation of the ACE2/Ang-(1-7)/Mas pathway reduces oxygen-glucose deprivation induced tissue swelling, ROS production, and cell death in mouse brain with angiotensin II overproduction

    PubMed Central

    Zheng, Jiaolin; Li, Guangze; Chen, Shuzhen; Chen, Ji; Buck, Joshua; Zhu, Yulan; Xia, Huijing; Lazartigues, Eric; Chen, Yanfang; Olson, James E.

    2014-01-01

    We previously demonstrated that mice which overexpress human renin and angiotensinogen (R+A+) show enhanced cerebral damage in both in vivo and in vitro experimental ischemia models. Angiotensin converting enzyme 2 (ACE2) counteracts the effects of angiotensin (Ang-II) by transforming it into Ang-(1-7), thus reducing the ligand for the AT1 receptor and increasing stimulation of the Mas receptor. Triple transgenic mice, SARA, which specifically overexpress ACE2 in neurons of R+A+ mice were used to study the role of ACE2 in ischemic stroke using oxygen and glucose deprivation (OGD) of brain slices as an in vitro model. We examined tissue swelling, the production of reactive oxygen species (ROS), and cell death in cerebral cortex (CX) and the hippocampal CA1 region during OGD. Expression levels of NADPH oxidase isoforms, Nox2 and Nox4 were measured using western blots. Results show that SARA mice and R+A+ mice treated with the Mas receptor agonist Ang-(1-7) had less swelling, cell death, and ROS production in CX and CA1 areas compared to those in R+A+ animals. Treatment of slices from SARA mice with the Mas antagonist A779 eliminated this protection. Finally, western blots revealed less Nox2 and Nox4 expression in SARA mice compared with R+A+ mice both before and after OGD. We suggest that reduced brain swelling and cell death observed in SARA animals exposed to OGD results from diminished ROS production coupled with lower expression of NADPH oxidases. Thus, the ACE2/Ang-(1-7)/Mas receptor pathway plays a protective role in brain ischemic damage by counteracting the detrimental effects of Ang-II-induced ROS production. PMID:24814023

  13. Activation of the ACE2/Ang-(1-7)/Mas pathway reduces oxygen-glucose deprivation-induced tissue swelling, ROS production, and cell death in mouse brain with angiotensin II overproduction.

    PubMed

    Zheng, J; Li, G; Chen, S; Bihl, J; Buck, J; Zhu, Y; Xia, H; Lazartigues, E; Chen, Y; Olson, J E

    2014-07-25

    We previously demonstrated that mice which overexpress human renin and angiotensinogen (R+A+) show enhanced cerebral damage in both in vivo and in vitro experimental ischemia models. Angiotensin-converting enzyme 2 (ACE2) counteracts the effects of angiotensin (Ang-II) by transforming it into Ang-(1-7), thus reducing the ligand for the AT1 receptor and increasing stimulation of the Mas receptor. Triple transgenic mice, SARA, which specifically overexpress ACE2 in neurons of R+A+ mice were used to study the role of ACE2 in ischemic stroke using oxygen and glucose deprivation (OGD) of brain slices as an in vitro model. We examined tissue swelling, the production of reactive oxygen species (ROS), and cell death in the cerebral cortex (CX) and the hippocampal CA1 region during OGD. Expression levels of NADPH oxidase (Nox) isoforms, Nox2 and Nox4 were measured using western blots. Results show that SARA mice and R+A+ mice treated with the Mas receptor agonist Ang-(1-7) had less swelling, cell death, and ROS production in CX and CA1 areas compared to those in R+A+ animals. Treatment of slices from SARA mice with the Mas antagonist A779 eliminated this protection. Finally, western blots revealed less Nox2 and Nox4 expression in SARA mice compared with R+A+ mice both before and after OGD. We suggest that reduced brain swelling and cell death observed in SARA animals exposed to OGD result from diminished ROS production coupled with lower expression of Nox isoforms. Thus, the ACE2/Ang-(1-7)/Mas receptor pathway plays a protective role in brain ischemic damage by counteracting the detrimental effects of Ang-II-induced ROS production. PMID:24814023

  14. Characterization of a new electrostatic storage ring for photofragmentation experiments

    SciTech Connect

    Pedersen, H. B. Svendsen, A.; Harbo, L. S.; Kiefer, H. V.; Kjeldsen, H.; Lammich, L.; Andersen, L. H.; Toker, Y.

    2015-06-15

    We describe the design of and the first commissioning experiments with a newly constructed electrostatic storage ring named SAPHIRA (Storage Ring in Aarhus for PHoton-Ion Reaction Analysis). With an intense beam of Cu{sup −} at 4 keV, the storage ring is characterized in terms of the stored ion beam decay rate, the longitudinal spreading of an injected ion bunch, as well as the direct measurements of the transverse spatial distributions under different conditions of storage. The ion storage stability in SAPHIRA was investigated systematically in a selected region of its electrical configuration space.

  15. Characterizing Student Experiences in Physics Competitions: The Power of Emotions

    NASA Astrophysics Data System (ADS)

    Moll, Rachel F.; Nashon, S.; Anderson, D.

    2006-12-01

    Low enrolment and motivation are key issues in physics education and recently the affective dimension of learning is being studied for evidence of its influence on student attitudes towards physics. Physics Olympics competitions are a novel context for stimulating intense emotional experiences. In this study, one team of students and their teacher were interviewed and observed prior to and during the event to characterize their emotions and determine the connections between their experiences and learning and attitudes/motivation towards physics. Results showed that certain types of events stimulated strong emotions of frustration and ownership, and that students’ attitudes were that physics is fun, diverse and relevant. Analysis of these themes indicated that the nature of emotions generated was connected to their attitudes towards physics. This finding points to the potential and value of informal and novel contexts in creating strong positive emotions, which have a strong influence on student attitudes towards physics.

  16. Safety rod/thimble melt failure characterization experiments

    SciTech Connect

    Stoots, C.M.; Hawkes, G.L.

    1992-05-01

    The Department of Energy (DOE) requested that he INEL perform experiments to study the thermal failure characteristics of a simulated Savannah River Site nuclear reactor safety rod and its surrounding thimble assembly. An electrically heated stainless steel rod simulated a reactor safety rod located eccentrically or concentrically within a perforated aluminum guide tube or thimble. A total of 37 experiments were conducted for a range of power levels and safety rod/thimble relative orientations. Video tapes were made of the four failure tests that were conducted to the melting point of the thimble. Although the primary emphasis of the experiments were to characterize the melting of the thimble qualitatively, experimental transient measurements included heater voltage and current, heater surface temperatures, aluminum thimble temperatures, and ambient temperature. Numerical studies were also performed in support of the experiments and data interpretation. Two finite element models were created to model the heat conduction-radiation between the stainless steel heater and thimble. The predicted temperatures were in good agreement with the experimental results.

  17. Experiment to characterize videos stored on the Web

    NASA Astrophysics Data System (ADS)

    Acharya, Soam; Smith, Brian C.

    1997-12-01

    The design of file systems is strongly influenced by measuring the use of existing file systems, such file size distribution and patterns of access. We believe that a similar characterization of video stored on the Internet will help network engineers, codec designers, and other multimedia researchers. We therefore executed an experiment to measure how video data is used on the Web today. In this experiment, we downloaded and analyzed over 57,000 AVI, QuickTime and MPEG files stored on the Web -- approximately 100 gigabytes of data. Among our more interesting discoveries, we found that the most common video technology in use today is QuickTime, and that the image resolution and frame rate of video files that include audio are much more uniform than video-only files. The majority of all audio/video files have dimensions of CIF or QCIF (or very similar) at 10, 12, 15, or 30 fps, whereas the dimensions and frame rates of video-only files are more uniformly distributed. We also experimentally verified the conjecture that current Internet bandwidth is at least an order of magnitude too slow to support streaming playback of video. We present these results and other statistical information characterizing video on the web in this paper.

  18. Shroud boundary condition characterization experiments at the Radiant Heat Facility.

    SciTech Connect

    Suo-Anttila, Jill Marie; Nakos, James Thomas; Gill, Walter

    2004-10-01

    A series of experiments was performed to better characterize the boundary conditions from an inconel heat source ('shroud') painted with Pyromark black paint. Quantifying uncertainties in this type of experimental setup is crucial to providing information for comparisons with code predictions. The characterization of this boundary condition has applications in many scenarios related to fire simulation experiments performed at Sandia National Laboratories Radiant Heat Facility (RHF). Four phases of experiments were performed. Phase 1 results showed that a nominal 1000 C shroud temperature is repeatable to about 2 C. Repeatability of temperatures at individual points on the shroud show that temperatures do not vary more than 10 C from experiment to experiment. This variation results in a 6% difference in heat flux to a target 4 inches away. IR camera images showed the shroud was not at a uniform temperature, although the control temperature was constant to about {+-}2 C during a test. These images showed that a circular shaped, flat shroud with its edges supported by an insulated plate has a temperature distribution with higher temperatures at the edges and lower temperatures in the center. Differences between the center and edge temperatures were up to 75 C. Phase 3 results showed that thermocouple (TC) bias errors are affected by coupling with the surrounding environment. The magnitude of TC error depends on the environment facing the TC. Phase 4 results were used to estimate correction factors for specific applications (40 and 63-mil diameter, ungrounded junction, mineral insulated, metal-sheathed TCs facing a cold surface). Correction factors of about 3.0-4.5% are recommended for 40 mil diameter TCs and 5.5-7.0% for 63 mil diameter TCs. When mounted on the cold side of the shroud, TCs read lower than the 'true' shroud temperature, and the TC reads high when on the hot side. An alternate method uses the average of a cold side and hot side TC of the same size to

  19. The SCITEAS experiment: Optical characterizations of sublimating icy planetary analogues

    NASA Astrophysics Data System (ADS)

    Pommerol, A.; Jost, B.; Poch, O.; El-Maarry, M. R.; Vuitel, B.; Thomas, N.

    2015-05-01

    We have designed and built a laboratory facility to investigate the spectro-photometric and morphologic properties of different types of ice-bearing planetary surface analogs and follow their evolution upon exposure to a low pressure and low temperature environment. The results obtained with this experiment are used to verify and improve our interpretations of current optical remote-sensing datasets. They also provide valuable information for the development and operation of future optical instruments. The Simulation Chamber for Imaging the Temporal Evolution of Analogue Samples (SCITEAS) is a small thermal vacuum chamber equipped with a variety of ports and feedthroughs that permit both in-situ and remote characterizations as well as interacting with the sample. A large quartz window located directly above the sample is used to observe its surface from outside with a set of visible and near-infrared cameras. The sample holder can be easily and quickly inserted and removed from the chamber and is compatible with the other measurement facilities of the Laboratory for Outflow Studies of Sublimating Materials (LOSSy) at the University of Bern. We report here on the results of two of the first experiments performed in the SCITEAS chamber. In the first experiment, fine-grained water ice mixed with dark organic and mineral matter was left to sublime in vacuum and at low temperature, simulating the evolution of the surface of a comet nucleus approaching the Sun. We observed and characterized the formation and evolution of a crust of refractory organic and mineral matter at the surface of the sample and linked the evolution of its structure and texture to its spectro-photometric properties. In the second experiment, a frozen soil was prepared by freezing a mixture of smectite mineral and water. The sample was then left to sublime for 6 h to simulate the loss of volatiles from icy soil at high latitudes on Mars. Colour images were produced using the definitions of the

  20. GPS Antenna Characterization Experiment (ACE): Receiver Design and Initial Results

    NASA Technical Reports Server (NTRS)

    Martzen, Phillip; Highsmith, Dolan E.; Valdez, Jennifer E.; Parker, Joel J. K.; Moreau, Michael C.

    2015-01-01

    The GPS Antenna Characterization Experiment (ACE) is a research collaboration between Aerospace and NASA Goddard to characterize the gain patterns of the GPS L1 transmit antennas. High altitude GPS observations are collected at a ground station through a transponder-based or "bent-pipe" architecture where the GPS L1 RF spectrum is received at a platform in geosynchronous orbit and relayed to the ground for processing. The focus of this paper is the unique receiver algorithm design and implementation. The high-sensitivity GPS C/A-code receiver uses high fidelity code and carrier estimates and externally supplied GPS message bit data in a batch algorithm with settings for a 0 dB-Hz threshold. The resulting carrier-to-noise measurements are used in a GPS L1 transmit antenna pattern reconstruction. This paper shows initial transmit gain patterns averaged over each block of GPS satellites, including comparisons to available pre-flight gain measurements from the GPS vehicle contractors. These results provide never-before-seen assessments of the full, in-flight transmit gain patterns.

  1. Design of Experiments for the Thermal Characterization of Metallic Foam

    NASA Technical Reports Server (NTRS)

    Crittenden, Paul E.; Cole, Kevin D.

    2003-01-01

    Metallic foams are being investigated for possible use in the thermal protection systems of reusable launch vehicles. As a result, the performance of these materials needs to be characterized over a wide range of temperatures and pressures. In this paper a radiation/conduction model is presented for heat transfer in metallic foams. Candidates for the optimal transient experiment to determine the intrinsic properties of the model are found by two methods. First, an optimality criterion is used to find an experiment to find all of the parameters using one heating event. Second, a pair of heating events is used to determine the parameters in which one heating event is optimal for finding the parameters related to conduction, while the other heating event is optimal for finding the parameters associated with radiation. Simulated data containing random noise was analyzed to determine the parameters using both methods. In all cases the parameter estimates could be improved by analyzing a larger data record than suggested by the optimality criterion.

  2. Characterizing the hohlraum radiation via one-end driven experiments

    SciTech Connect

    Zhao, Yiqing; Zou, Shiyang; Li, Sanwei; Li, Zhichao; Guo, Liang

    2014-07-15

    A new experiment is designed and performed on the Shenguang III laser facility with the first eight available beams to characterizing the hohlraum radiation, in which the hohlraum with laser entrance holes on both ends is driven through one-end only. The experiment enables us to identify the x-ray radiations originated from the hohlraum reemission wall and high-Z bubble plasmas utilizing their position and spectral characters, which provides a better test on the associated hohlraum models. The total and M-band x-ray radiation fluxes are measured with the flat response x-ray detectors and the filtered M-band x-ray detectors, respectively. Numerical simulations are conducted with the two-dimensional radiation hydrodynamic code LARED-INTEGRATION using the multi-group radiation transfer and/or diffusion models. It is found that the experimentally measured temporal profiles and angular distributions of hohlraum radiation are in good agreement with the predictions of simulation using radiation transfer models, but differ significantly from the results obtained with the multi-group radiation diffusion calculations. We thus note that to accurately represent the hohlraum radiation, a true radiation transfer model is essential.

  3. A CHI wiggler ubitron amplifier experiment: Wiggler characterization

    SciTech Connect

    Taccetti, J.M.; Jackson, R.H.; Freund, H.P.

    1995-12-31

    A 35 GHz CHI (Coaxial Hybrid Iron) wiggler ubitron amplifier experiment is under construction at the Naval Research Laboratory. The CHI wiggler configuration has the potential of generating high wiggler magnetic fields at short periods with excellent beam focusing and transport properties. This makes it a desirable configuration for the generation of high power coherent radiation in relatively compact systems. The CHI wiggler consists of alternating rings of magnetic and non-magnetic materials concentric with a central rod of similar alternating design but shifted along the axis by half a period. Once inserted in a solenoidal magnetic field, the CHI structure deforms the axial field to create a radial field oscillating with the same periodicity as the rings. An annular electron beam is propagated through the coaxial gap where the oscillating radial field imparts an azimuthal wiggle motion. The principal goals of the experiment are to investigate the performance tradeoffs involved in the CHI configuration for high frequency amplifiers operating at low voltages with small wiggler periods. The nominal design parameters are a center frequency of 35 GHz, wiggler period of 0.75 cm, and beam voltage of approximately 150 kV. Calculations have shown an intrinsic (untapered) efficiency of {approximately} 7% when operating at 6.3 kG axial field (wiggler field, B{sub w}{approximately}1270 G). The calculated gain was 36 dB, saturating at a distance of 46 cm. These parameters yield an instantaneous amplifier bandwidth of {approximately} 25%. There appears to be room for further improvement in efficiency, a matter which will be scrutinized more closely in the final design. A prototype CHI wiggler is presently being fabricated for use in conjunction with an existing 30 kG superconducting solenoid. The performance properties of the prototype will be characterized and compared with linear and non-linear calculations.

  4. Protective Effect of RA on Myocardial Infarction-Induced Cardiac Fibrosis via AT1R/p38 MAPK Pathway Signaling and Modulation of the ACE2/ACE Ratio.

    PubMed

    Liu, Qiaofeng; Tian, Jingwei; Xu, Yanan; Li, Chunmei; Meng, Xiangjing; Fu, Fenghua

    2016-09-01

    Rosmarinic acid (α-o-caffeoyl-3,4-dihydroxyphenyllactic acid, RA) is a major active constituent of Rosmarinus officinalis Linn. (rosemary) having significant anti-inflammatory, anti-apoptotic, and antioxidant effects. However, the cardioprotection of RA is still not understood. The present study was designed, for the first time, to investigate the cardioprotection of RA on myocardial infarction (MI)-induced cardiac fibrosis and to clarify the possible mechanisms. MI was induced in adult rats by left anterior descending coronary artery ligation, and animals were then administered RA (50, 100, or 200 mg/kg) by gavage. Compared with the model group, RA treatment ameliorated changes in the left ventricular systolic pressure (LVSP), +dp/dtmax, and -dp/dtmax after 4 weeks. This was associated with attenuation of infarct size, collagen volume fraction (CVF), expression of collagen I, collagen III, alpha smooth muscle actin (α-SMA), and hydroxyproline (Hyp) concentrations. RA treatment was also associated with decreased angiotensin-converting enzyme (ACE) expression and increased ACE2 expression, as well as decreased expression of angiotensin type 1 receptor (AT1R) and phospho-p38 mitogen-activated protein kinase (p38 MAPK). Thus, RA can protect against cardiac dysfunction and fibrosis following MI, likely due to decreasing ACE expression and increasing ACE2 expression via the AT1R/p38 MAPK pathway. PMID:27538767

  5. Characterization of the homogeneous reactor experiment No. 2 (HRE) impoundment

    SciTech Connect

    Stansfield, R.G.; Francis, C.W.

    1986-07-01

    A characterization study was conducted on a radioactive waste impoundment for the Homogeneous Reactor Experiment No. 2 at the Oak Ridge National Laboratory, to provide information necessary for its proper disposition. The impoundment received low-level radioactive wastes from 1957 until 1963. In 1970, the pond was backfilled with clay soil and the impoundment capped with asphaltic concrete, but no wastes were removed. The mixed soil fill and sediment, approximately 4.8 m deep, was sampled using soil boring methods. The samples were analyzed to determine if the material would classify as a hazardous waste under regulatory definitions promulgated in accordance with the Resource Conservation and Recovery Act (RCRA). If the soil and sediment mixture contained RCRA-defined hazardous waste, it would be subject to the Comprehensive Environmental Response, Compensation and Liability Act (CERCLA). Chemical analyses indicate that the sampled material does not contain hazardous chemical constituents above the levels permitted by RCRA regulations. The sediment was found to contain an estimated radioactivity inventory of approximately 2700 GBq (70 Ci) of /sup 90/Sr and 600 GBq (16 Ci) of /sup 137/Cs.

  6. Characterization of segmented Silicon detectors for neutron beta decay experiments

    NASA Astrophysics Data System (ADS)

    Salas, Americo; McGaughey, Patrick

    2012-03-01

    The ``Nab'', and ``UCNB'' collaborations will measure the correlation parameters ``a'', ``b'', and ``B'' that are found in the triple differential rate equation from neutron β-decay ( n ->p + e + νe). These parameters that offer an atractive platform for searches of signals of new physics beyond standard model will be measured using unpolarized cold neutrons (Nab) at SNS, ORNL,and polarized ultracold neutrons (UCNB) at LANL. Following a neutron β-decay the electron and proton, will be accelerated in a 4π-field spectrometer, and detected by a novel detector design consisting of two opposite large area and thick silicon detectors segmented in 127 pixels per detector, and operated at ˜ 100 Kelvin. We have successfully completed the first phase of detector characterization, operating 0.5, 1.0, and 1.5 mm thick Silicon detectors of 11 cm in diameter for neutron β-decay experiments at Los Alamos National Laboratory, and detected ˜ 300 Hz protons from 15 to 35 keV at NCSU with a FWHM resolution of ˜ 3.2 keV with a potential of another factor of two improvement. Custom amplifiers based on FETs mounted directly on the detector reduced the noise and made possible the proton detection.

  7. Study to perform preliminary experiments to evaluate particle generation and characterization techniques for zero-gravity cloud physics experiments

    NASA Technical Reports Server (NTRS)

    Katz, U.

    1982-01-01

    Methods of particle generation and characterization with regard to their applicability for experiments requiring cloud condensation nuclei (CCN) of specified properties were investigated. Since aerosol characterization is a prerequisite to assessing performance of particle generation equipment, techniques for characterizing aerosol were evaluated. Aerosol generation is discussed, and atomizer and photolytic generators including preparation of hydrosols (used with atomizers) and the evaluation of a flight version of an atomizer are studied.

  8. Viscoplastic Characterization of Ti-6-4: Experiments

    NASA Technical Reports Server (NTRS)

    Lerch, Bradley A.; Arnold, Steven M.

    2016-01-01

    As part of a continued effort to improve the understanding of material time-dependent response, a series of mechanical tests have been conducted on the titanium alloy, Ti-6Al-4V. Tensile, creep, and stress relaxation tests were performed over a wide range of temperatures and strain rates to engage various amounts of time-dependent behavior. Additional tests were conducted that involved loading steps, overloads, dwell periods, and block loading segments to characterize the interaction between plasticity and time-dependent behavior. These data will be used to characterize a recently developed, viscoelastoplastic constitutive model with a goal toward better estimates of aerospace component behavior, resulting in improved safety.

  9. Thermal Characterization of Functionally Graded Materials: Design of Optimum Experiments

    NASA Technical Reports Server (NTRS)

    Cole, Kevin D.

    2003-01-01

    This paper is a study of optimal experiment design applied to the measure of thermal properties in functionally graded materials. As a first step, a material with linearly-varying thermal properties is analyzed, and several different tran- sient experimental designs are discussed. An optimality criterion, based on sen- sitivity coefficients, is used to identify the best experimental design. Simulated experimental results are analyzed to verify that the identified best experiment design has the smallest errors in the estimated parameters. This procedure is general and can be applied to design of experiments for a variety of materials.

  10. Characterizing Variability in Smestad and Gratzel's Nanocrystalline Solar Cells: A Collaborative Learning Experience in Experimental Design

    ERIC Educational Resources Information Center

    Lawson, John; Aggarwal, Pankaj; Leininger, Thomas; Fairchild, Kenneth

    2011-01-01

    This article describes a collaborative learning experience in experimental design that closely approximates what practicing statisticians and researchers in applied science experience during consulting. Statistics majors worked with a teaching assistant from the chemistry department to conduct a series of experiments characterizing the variation…

  11. Communications Link Characterization Experiment (CLCE) technical data report, volume 2

    NASA Technical Reports Server (NTRS)

    1977-01-01

    The results are presented of the long term rain rate statistical analysis and the investigation of determining the worst month statistical from the measured attenuation data caused by precipitation. The rain rate statistics cover a period of 11 months from July of 1974 to May of 1975 for measurements taken at the NASA, Rosman station. The rain rate statistical analysis is a continuation of the analysis of the rain rate data accumulated for the ATS-6 Millimeter Wave Progation Experiment. The statistical characteristics of the rain rate data through December of 1974 is also presented for the above experiment.

  12. Source term experiments project (STEP): aerosol characterization system

    SciTech Connect

    Schlenger, B.J.; Dunn, P.F.

    1985-01-01

    A series of four experiments is being conducted at Argonne National Laboratory's TREAT Reactor. They have been designed to provide some of the necessary data regarding magnitude and release rates of fission products from degraded fuel pins, physical and chemical characteristics of released fission products, and aerosol formation and transport phenomena. These are in-pile experiments, whereby the test fuel is heated by neutron induced fission and subsequent clad oxidation in steam environments that simulate as closely as practical predicted reactor accident conditions. The test sequences cover a range of pressure and fuel heatup rate, and include the effect of Ag/In/Cd control rod material.

  13. Optimal Experiment Design for Thermal Characterization of Functionally Graded Materials

    NASA Technical Reports Server (NTRS)

    Cole, Kevin D.

    2003-01-01

    The purpose of the project was to investigate methods to accurately verify that designed , materials meet thermal specifications. The project involved heat transfer calculations and optimization studies, and no laboratory experiments were performed. One part of the research involved study of materials in which conduction heat transfer predominates. Results include techniques to choose among several experimental designs, and protocols for determining the optimum experimental conditions for determination of thermal properties. Metal foam materials were also studied in which both conduction and radiation heat transfer are present. Results of this work include procedures to optimize the design of experiments to accurately measure both conductive and radiative thermal properties. Detailed results in the form of three journal papers have been appended to this report.

  14. PREPARATION AND CHARACTERIZATION OF FINELY DIVIDED PARTICULATE ENVIRONMENTAL CONTAMINANTS FOR BIOLOGICAL EXPERIMENTS

    EPA Science Inventory

    The purpose of this project was to provide a centralized source for the preparation and characterization of selected particulate materials for biological experiments. The particulate materials of interest were a range of environmental contaminants known or suspected to detrimenta...

  15. Characterizing the sexual abuse experiences of young adolescents

    PubMed Central

    Negriff, Sonya; Schneiderman, Janet U.; Smith, Caitlin; Schreyer, Justine K.; Trickett, Penelope K.

    2013-01-01

    The purpose of this descriptive study was to: (a) compare the demographics of maltreated youth initially labeled as sexually abused by the Department of Child and Family Services (DCFS) to maltreated youth classified as sexually abused using current and past case records, (b) identify differences in sexual abuse experiences and types of perpetrators between boys and girls, and (c) provide a detailed description of the sexual abuse experiences for boys and girls. Participants were youth ages 9–12 years old with a recent maltreatment allegation. The Maltreatment Case Record Abstraction Instrument (MCRAI) was used to code child welfare records of 303 maltreated youth of whom 60 experienced sexual abuse. Perpetrators were classified by gender into four categories (biological parent, parental figure, relative, and unrelated) and type of abuse was classified into three categories (penetrative, contact without penetration, and non-contact). Using Chi-Square tests, perpetrator categories and sexual abuse types were compared by child gender for significant differences. Only 23 (38.3%) of the 60 sexually abused youth were labeled as sexually abused in the most recent DCFS report when they entered the study. About three-quarters of the sexually abused youth experienced non-penetrative physical contact, 40% experienced penetration, and 15% experienced sexual abuse without physical contact. Most youth (91.7%) were victimized by a male, and 21.7% were abused by a female. Youth experienced a large range of sexual abuse experiences, the details of which may be important for exploration of consequences of childhood sexual abuse. PMID:24095179

  16. Testing the BUNDLE Experiment for Microgravity Disturbance Characterization

    NASA Technical Reports Server (NTRS)

    Gattis, Christy; Engberg, Bob

    2000-01-01

    A primary objective of the International Space Station is to provide a premier facility for microgravity research. There are many disturbances on board the Station that can disturb the quality of the microgravity environment, including disturbances caused by both system and payload hardware. Disturbances within experiments themselves can also affect the quality of their own environment. The primary functions of the Bridgman Unidirectional Dendrites in Liquids Experiment (BUNDLE) are to melt, directionally solidify, and perform in-situ quench of metallic alloys, providing data to aid in understanding the effects of the microgravity environment on the characteristics of these processed metals. Quenching of the samples in the BUNDLE furnace is accomplished by rapidly introducing high pressure helium into the furnace chamber. This type of quench will induce sample vibrations which may be large enough to adversely affect sample quality. The primary objective of the test described in this presentation was to determine the acceleration imparted on the BUNDLE sample during helium quench. From this information, the science community can better assess whether this method of quench will allow them to obtain the data they need. Utilizing breadboard hardware, the sample quench sequence was conducted. Data was collected from accelerometers located on the breadboard hardware and inside the sample cartridge. The test data indicated that the maximum acceleration achieved by the sample was 0.325 g, which is much higher than the desired microgravity (10(exp -6) g) environment. However, this acceleration level occurred over a time period of less than 0.1 second. Because this time period is so short, there should be no detrimental effects to dendrite growth. This result was consistent with the observations of the experiment scientists, who are satisfied with the results of previous dendrite growth in this breadboard unit.

  17. The Synthesis and Characterization of Some Fluoride Perovskites: An Undergraduate Experiment in Solid State Chemistry.

    ERIC Educational Resources Information Center

    Langley, Richard H.; And Others

    1984-01-01

    Describes a senior-level experiment dealing with the synthesis and characterization of a perovskite. Since most perovskites are cubic, their characterization by x-ray diffraction is simplified. In addition, magnetic ordering may be observed and the effects of a Jahn-Teller distortion seen. (JN)

  18. Experimental Characterization of Plasma Flow in Reconnection Scaling Experiment.

    NASA Astrophysics Data System (ADS)

    Dorf, L.; Sun, X.; Intrator, T.; Hendryx, J.; Wurden, G.

    2007-11-01

    Reconnection Scaling Experiment (RSX) studies linear and non-linear evolution of up to four interacting current-carrying plasma cords with emphasis on kink instability and magnetic reconnection. During the kink instability, the presence of an axial flow gives rise to a Doppler shifted frequency and rotation of the kink, which makes studying the flow important. The axial velocity, plasma density, and electron temperature in one plasma column were measured on RSX with the miniaturized Mach and triple electrostatic probes installed on 3D positioning systems. Significant plasma flow with the velocity on the order of the ion acoustic speed was detected, with the velocity decreasing downstream. 2D profiles obtained at two axial locations were then employed to estimate the radial profile of the ion viscosity using the integral momentum balance equation. The results show that the ion momentum flux is dissipated by the ion-ion viscosity due to significant radial shear of axial velocity. Chord-integrated ion temperature measurements performed at several radial locations using Doppler broadening spectroscopy show temperature of about 1eV. Comparison of the measured viscosity with Braginskii's theoretical predictions demonstrates a good agreement, which is an important new result useful for both astrophysical jets and magnetoplasmadynamic thrusters. Supported by OFES, and DOE/LANL contract DE-AC52-06NA25396.

  19. Characterization of the Earth Radiation Budget Experiment radiometers

    NASA Technical Reports Server (NTRS)

    Lee, R. B., III; Barkstrom, B. R.

    1991-01-01

    The Earth Radiation Budget Experiment (ERBE) scanning radiometers were used to measure the earth's radiation fields during the period November 1984 through February 1990. The ERBE radiometric packages were placed into orbit aboard the Earth Radiation Budget Satellite (ERBS) and the NOAA-9 and NOAA-10 spacecraft platforms. In each radiometric package, thermistor bolometers were used as detection elements for the broadband total (0,2 - 50,0 microns), shortwave (0,2 - 5,0 microns), and longwave (5,0 - 50,0 microns) spectral regions. Flight calibration facilities were built into each of the spacecraft radiometric packages. The flight facilities consisted of black bodies, tungsten lamps, and silicon photodiodes. The black bodies and tungsten lamps were found to be reliable at precision levels approaching 0,5 percent over a five-year period. The photodiodes were found to degrade more than 2 percent during the first year in orbit. In this paper, the flight calibration systems for the ERBE scanning radiometers are described along with the resultant measurements.

  20. Characterization of materials for a reactive transport model validation experiment: Interim report on the caisson experiment. Yucca Mountain Site Characterization Project

    SciTech Connect

    Siegel, M.D.; Cheng, W.C.; Ward, D.B.; Bryan, C.R.

    1995-08-01

    Models used in performance assessment and site characterization activities related to nuclear waste disposal rely on simplified representations of solute/rock interactions, hydrologic flow field and the material properties of the rock layers surrounding the repository. A crucial element in the design of these models is the validity of these simplifying assumptions. An intermediate-scale experiment is being carried out at the Experimental Engineered Test Facility at Los Alamos Laboratory by the Los Alamos and Sandia National Laboratories to develop a strategy to validate key geochemical and hydrological assumptions in performance assessment models used by the Yucca Mountain Site Characterization Project.

  1. Southern Great Plains Ice Nuclei Characterization Experiment Final Campaign Summary

    SciTech Connect

    DeMott, PJ; Suski, KJ; Hill, TCJ; Levin, EJT

    2015-03-01

    The first ever ice nucleating particle (INP) measurements to be collected at the Southern Great Plains site were made during a period from late April to June 2014, as a trial for possible longer-term measurements at the site. These measurements will also be used to lay the foundation for understanding and parameterizing (for cloud resolving modeling) the sources of these climatically important aerosols as well as to augment the existing database containing this knowledge. Siting the measurements during the spring was intended to capture INP sources in or to this region from plant, soil, dust transported over long distances, biomass burning, and pollution aerosols at a time when they may influence warm-season convective clouds and precipitation. Data have been archived of real-time measurements of INP number concentrations as a function of processing conditions (temperature and relative humidity) during 18 days of sampling that spanned two distinctly different weather situations: a warm, dry and windy period with regional dust and biomass burning influences in early May, and a cooler period of frequent precipitation during early June. Precipitation delayed winter wheat harvesting, preventing intended sampling during that perturbation on atmospheric aerosols. INP concentrations were highest and most variable at all temperatures in the dry period, where we attribute the INP activity primarily to soil dust emissions. Additional offline INP analyses are underway to extend the characterization of INP to cover the entire mixed phase cloud regime from -5°C to -35°C during the full study. Initial comparisons between methods on four days show good agreement and excellent future promise. The additional offline immersion freezing data will be archived as soon as completed under separate funding. Analyses of additional specialized studies for specific attribution of INP to biological and smoke sources are continuing via the National Science Foundation and National Aeronautics

  2. Optical Microscopy Characterization for Borehole U-15n#12 in Support of NCNS Source Physics Experiment

    SciTech Connect

    Wilson, Jennifer E.; Sussman, Aviva Joy

    2015-05-22

    Optical microscopy characterization of thin sections from corehole U-15n#12 is part of a larger material characterization effort for the Source Physics Experiment (SPE). The SPE program was conducted in Nevada with a series of explosive tests designed to study the generation and propagation of seismic waves inside Stock quartz monzonite. Optical microscopy analysis includes the following: 1) imaging of full thin sections (scans and mosaic maps); 2) high magnification imaging of petrographic texture (grain size, foliations, fractures, etc.); and 3) measurement of microfracture density.

  3. Characterizing W-2 SLSF experiment temperature oscillations using computer graphics. [Sodium Loop Safety Facility

    SciTech Connect

    Smith, D.E.

    1983-06-23

    The W-2 SLSF (Sodium Loop Safety Facility) experiment was an instrumented in-reactor test performed to characterize the failure response of full-length, preconditioned LMFBR prototypic fuel pins to slow transient overpower (TOP) conditions. Although the test results were expected to confirm analytical predictions of upper level failure and fuel expulsion, an axial midplane failure was experienced. Extensive post-test analyses were conducted to understand all of the unexpected behavior in the experiment. (1) The initial post-test effort focused on the temperature oscillations recorded by the 54 thermocouples used in the experiment. In order to synthesize the extensive data records and identify patterns of behavior in the data records, a computer-generated film was used to present the temperature data recorded during the experiment.

  4. Magic angle spinning solid-state NMR experiments for structural characterization of proteins.

    PubMed

    Shi, Lichi; Ladizhansky, Vladimir

    2012-01-01

    Solid-state nuclear magnetic resonance (SSNMR) has become a prominent method in biology and is suitable for the characterization of insoluble proteins and protein aggregates such as amyloid fibrils, membrane-lipid complexes, and precipitated proteins. Often, the initial and the most critical step is to obtain spectroscopic assignments, that is, to determine chemical shifts of individual atoms. The procedures for SSNMR spectroscopic assignments are now well established for small microcrystalline proteins, where high signal-to-noise can be obtained. The sensitivity of the experiments and spectral resolution decrease with the increasing molecular weight, which makes setting SSNMR experiments in large proteins a much more challenging and demanding procedure. Here, we describe the protocol for the most common set of 3D magic angle spinning (MAS) SSNMR experiments. While the procedures described in the text are well known to SSNMR practitioners, we hope they will be of interest to scientists interested in extending their repertoire of biophysical techniques. PMID:22760319

  5. Iterative experiment design guides the characterization of a light-inducible gene expression circuit

    PubMed Central

    Ruess, Jakob; Parise, Francesca; Milias-Argeitis, Andreas; Khammash, Mustafa; Lygeros, John

    2015-01-01

    Systems biology rests on the idea that biological complexity can be better unraveled through the interplay of modeling and experimentation. However, the success of this approach depends critically on the informativeness of the chosen experiments, which is usually unknown a priori. Here, we propose a systematic scheme based on iterations of optimal experiment design, flow cytometry experiments, and Bayesian parameter inference to guide the discovery process in the case of stochastic biochemical reaction networks. To illustrate the benefit of our methodology, we apply it to the characterization of an engineered light-inducible gene expression circuit in yeast and compare the performance of the resulting model with models identified from nonoptimal experiments. In particular, we compare the parameter posterior distributions and the precision to which the outcome of future experiments can be predicted. Moreover, we illustrate how the identified stochastic model can be used to determine light induction patterns that make either the average amount of protein or the variability in a population of cells follow a desired profile. Our results show that optimal experiment design allows one to derive models that are accurate enough to precisely predict and regulate the protein expression in heterogeneous cell populations over extended periods of time. PMID:26085136

  6. Well-characterized open pool experiment data and analysis for model validation and development.

    SciTech Connect

    Sundberg, David W.; Brown, Alexander L.; Blanchat, Thomas K.

    2006-12-01

    Four Well-Characterized Open Pool fires were conducted by Fire Science and Technology Department. The focus of the Well-Characterized Open Pool fire series was to provide environmental information for open pool fires on a physics first principal basis. The experiments measured the burning rate of liquid fuel in an open pool and the resultant heat flux to a weapon-sized object and the surrounding environment with well-characterized boundary and initial conditions. Results presented in this report include a general description of test observation (pre- and post-test), wind measurements, fire plume topology, average fuel recession and heat release rates, and incident heat flux to the pool and to the calorimeters. As expected, results of the experiments show a strong correlation between wind conditions, fuel vaporization (mass loss) rate, and incident heat flux to the fuel and ground surface and calorimeters. Numerical fire simulations using both temporally- and spatially-dependant wind boundary conditions were performed using the Vulcan fire code. Comparisons of data to simulation predictions showed similar trends; however, simulation-predicted incident heat fluxes were lower than measured.

  7. Implementation and automation of a Faraday experiment for the magneto-optical characterization of ferrofluids

    NASA Astrophysics Data System (ADS)

    Velásquez, A. A.; Urquijo, J. P.

    2016-01-01

    This work presents the design, assembly and automation of a Faraday experiment for use in characterization of the magneto-optical response of fluids and ferrofluids. The magneto-optical Faraday experiment was automated using programmable equipment, controlled through the IEEE-488 port via Standard Commands for Programmable Instruments executed from a graphical interface developed in LabVIEW software. To calibrate the system the Verdet constants of distilled water and isopropyl alcohol were measured, obtaining an error percentage less than 2% for both fluids. Subsequently we used the system for measuring the Verdet constant of a ferrofluid of iron oxide nanoparticles diluted in distilled water, which was synthesized and, before its dilution, characterized by scanning electron microscopy, room temperature Mössbauer spectroscopy and vibrating sample magnetometry. We found that the Verdet constant of the diluted ferrofluid was smaller than that of distilled water, indicating opposite contributions of the effects of the diamagnetic and paramagnetic phases present in the ferrofluid to the magneto-optical effect. Details of the assembly, control of the experiment and development of the measurements are presented in this paper.

  8. Geometric Approximation: A New Computational Approach To Characterize Protein Dynamics from NMR Adiabatic Relaxation Dispersion Experiments.

    PubMed

    Chao, Fa-An; Byrd, R Andrew

    2016-06-15

    A new computational strategy is reported that provides a fast approximation of numerical solutions of differential equations in general. The method is demonstrated with the analysis of NMR adiabatic relaxation dispersion experiments to reveal biomolecular dynamics. When an analytical solution to the theoretical equations describing a physical process is not available, the new approach can significantly accelerate the computational speed of the conventional numerical integration up to 10(5) times. NMR adiabatic relaxation dispersion experiments enhanced with optimized proton-decoupled pulse sequences, although extremely powerful, have previously been refractory to quantitative analysis. Both simulations and experimental validation demonstrate detectable "slow" (microsecond to millisecond) conformational exchange rates from 10(2) to 10(5) s(-1). This greatly expanded time-scale range enables the characterization of a wide array of conformational fluctuations for individual residues, which correlate with biomolecular function and were previously inaccessible. Moreover, the new computational method can be potentially generalized for analysis of new types of relaxation dispersion experiments to characterize the various dynamics of biomolecular systems. PMID:27225523

  9. Characterization of DNA-protein interactions using high-throughput sequencing data from pulldown experiments

    NASA Astrophysics Data System (ADS)

    Moreland, Blythe; Oman, Kenji; Curfman, John; Yan, Pearlly; Bundschuh, Ralf

    Methyl-binding domain (MBD) protein pulldown experiments have been a valuable tool in measuring the levels of methylated CpG dinucleotides. Due to the frequent use of this technique, high-throughput sequencing data sets are available that allow a detailed quantitative characterization of the underlying interaction between methylated DNA and MBD proteins. Analyzing such data sets, we first found that two such proteins cannot bind closer to each other than 2 bp, consistent with structural models of the DNA-protein interaction. Second, the large amount of sequencing data allowed us to find rather weak but nevertheless clearly statistically significant sequence preferences for several bases around the required CpG. These results demonstrate that pulldown sequencing is a high-precision tool in characterizing DNA-protein interactions. This material is based upon work supported by the National Science Foundation under Grant No. DMR-1410172.

  10. Characterization of novel Hamamatsu Multi Pixel Photon Counter (MPPC) arrays for the GlueX experiment

    SciTech Connect

    Soto, Orlando; Rojas, Rimsky; Kuleshov, Sergey V.; Hakobyan, Hayk; Toro, Alam; Brooks, William K.

    2013-12-01

    The novel Hamamatsu Multi Pixel Photon Counter Array S12045(X) is an array of 16 individual MPPCs (3x3 mm{sup 2}) (further in the paper MPPC array channel) each with 3600 G-APD (Geiger-mode Avalanche Photodiodes) pixels (50x50 [{micro}m{sup 2}]). Each MPPC in the array works with its individual reverse bias voltage mode (around 70 V). The paper summarizes the characterization process of MPPC arrays used in GlueX experiment (Hall D, Jefferson Lab). We studied the main features of each MPPC array channel for 2800 MPPC arrays at different temperatures. Two measurement stations were built to extract gain, breakdown voltage, photo detection efficiency (PDE), optical crosstalk and dark rate for each MPPC array channel. The hardware and the data analysis are described, which includes new analytical expressions to obtain the mean number of photo-electrons and optical crosstalk. The dynamical behavior of characterization parameters is presented as well.

  11. Preliminary stress characterization for an in-situ stimulation experiment at the Grimsel Underground Laboratory

    NASA Astrophysics Data System (ADS)

    Krietsch, Hannes; Doetsch, Joseph; Gischig, Valentin; Amann, Florian; Jalali, Mohammadreza; Madonna, Claudio; Evans, Keith; Valley, Benoit; Giardini, Domenico; Wiemer, Stefan; Maurer, Hansruedi; Loew, Simon

    2016-04-01

    A decameter-scale in-situ stimulation experiment is currently being executed at the Grimsel Test Site in Switzerland, spanning from hydraulic fracturing to controlled fault-slip experiments. For the feasibility of this project the in-situ stress tensor is of foremost importance. Therefore a unique stress characterization campaign combining stress relief methods (overcoring of USBM and CSIRO-HI probes) with hydraulic fracturing (HF) and hydraulic testing on pre-existing fractures (HTPF) in three boreholes was conducted in a first phase of this project. During all hydraulic stress measurements, micro-seismicity was monitored and localized in real time utilizing a dense network of piezo-electric sensors. In this contribution, we present preliminary results of the stress characterization and compare the derived stress tensor with previous estimates of the stress state. The stress characterization campaign was conducted in three boreholes, one sub-vertical and two sub-horizontal boreholes, assuming that the sub-vertical and one sub-horizontal are parallel to a principal stress component. A major task in this contribution is the integration of the different stress characterization methods. Our results of the different methods (overcoring and HF) are largely consistent, but disagree with some of the previous stress orientation estimates. From the new campaign the overcoring measurements indicate a sub-horizontal sigma1 of 17.3 MPa with a strike of 145°, a sigma2 of 9.7 MPa with 241°/69° and a sigma3 of 8.3 MPa with 055°/21° using an isotropic approach for inversion calculation. Whereas the USBM-Probe measures a projection of the principal stresses in a plane normal to borehole axis, the CSIRO-HI Probe provides the real 3D stress tensor. The HF and HTPF measurements indicate a far-field minimum horizontal stress between 8.7 and 9.1 MPa, consistent with the overcoring. Principal stresses, estimated by location of micro-seismic events during HF and HTPF, suggest that

  12. Recent Experience Using Active Love Wave Techniques to Characterize Seismographic Station Sites

    NASA Astrophysics Data System (ADS)

    Martin, A. J.; Yong, A.; Salomone, L.

    2014-12-01

    Active-source Love waves recorded by the multi-channel analysis of surface wave (MASLW) technique were recently analyzed in two site characterization projects. Between 2010 and 2011, the 2009 American Recovery and Reinvestment Act (ARRA) funded GEOVision to conduct geophysical investigations at 189 seismographic stations—185 in California and 4 in the Central Eastern U.S. (CEUS). The original project plan was to utilize active and passive Rayleigh wave-based techniques to obtain shear-wave velocity (VS) profiles to a minimum depth of 30 m and the time-averaged VS of the upper 30 meters (VS30). Early in the investigation it became evident that Rayleigh wave techniques, such as multi-channel analysis of surface waves (MASRW), were not effective at characterizing all sites. Shear-wave seismic refraction and MASLW techniques were therefore applied. The MASLW technique was deployed at a total of 38 sites, in addition to other methods, and used as the primary technique to characterize 22 sites, 5 of which were also characterized using Rayleigh wave techniques. In 2012, the Electric Power Research Institute funded characterization of 33 CEUS station sites. Based on experience from the ARRA investigation, both MASRW and MASLW data were acquired by GEOVision at 24 CEUS sites—the remaining 9 sites and 2 overlapping sites were characterized by University of Texas, Austin. Of the 24 sites characterized by GEOVision, 16 were characterized using MASLW data, 4 using both MASLW and MASRW data and 4 using MASRW data. Love wave techniques were often found to perform better, or at least yield phase velocity data that could be more readily modeled using the fundamental mode assumption, at shallow rock sites, sites with steep velocity gradients, and, sites with a thin, low velocity, surficial soil layer overlying stiffer sediments. These types of velocity structure often excite dominant higher modes in Rayleigh wave data, but not in Love wave data. At such sites, it may be possible

  13. A possible experiment at LEUTL to characterize surface roughness Wakefield effects

    NASA Astrophysics Data System (ADS)

    Biedron, Sandra G.; Dattoli, Giuseppe; Fawley, William M.; Freund, Henry P.; Huang, Zhirong; Lewellen, John W.; Milton, Stephen V.; Nuhn, Heinz-Dieter

    2001-08-01

    Wakefield effects due to internal vacuum chamber roughness may increase the electron beam energy spread and so have become an immediate concern for future x-ray free-electron laser (FEL) project developments such as the SLAC Linac Coherent Light Source (LCLS) and the DESY TESLA x-ray FEL. We describe a possible experiment to characterize the effects of surface roughness on an FEL driven by self-amplified spontaneous emission (SASE) operation. Although the specific system described is not completely identical to the above-proposed projects, much useful scaling information could be obtained and applied to shorter wavelength systems.

  14. Characterizing a proton beam with two different methods in beam halo experiments

    NASA Astrophysics Data System (ADS)

    Jiang, Hong-Ping; Fu, Shi-Nian; Peng, Jun; Cheng, Peng; Huang, Tao; Li, Peng; Li, Fang; Li, Jian; Liu, Hua-Chang; Liu, Mei-Fei; Meng, Ming; Meng, Cai; Mu, Zhen-Cheng; Rong, Lin-Yan; Ouyang, Hua-Fu; Sun, Biao; Wang, Bo; Tian, Jian-Min; Wang, Biao; Wang, Sheng-Chang; Yao, Yuan; Xu, Tao-Guang; Xu, Xin-An; Xin, Wen-Qu; Zhao, Fu-Xiang; Zeng, Lei; Zhou, Wen-Zhong

    2014-08-01

    In beam halo experiments, it is very important to correctly characterize the RFQ output proton beam. In order to simulate the beam dynamics properly, we must first know the correct initial beam parameters. We have used two different methods, quadrupole scans and multi-wire scanners to determine the transverse phase-space properties of the proton beam. The experimental data were analyzed by fitting to the 3-D nonlinear simulation code IMPACT. For the quadrupole scan method, we found that the RMS beam radius and the measured beam-core profiles agreed very well with the simulations. For the multi-wire scanner method, we choose the case of a matched beam. By fitting the IMPACT simulation results to the measured data, we obtained the Courant-Snyder parameters and the emittance of the beam. The difference between the two methods is about eight percent, which is acceptable in our experiments.

  15. Procedure development study: Low strain rate and creep experiments; Yucca Mountain Site Characterization Project

    SciTech Connect

    Martin, R.J. III; Boyd, P.J.; Noel, J.S.; Price, R.H.

    1991-10-01

    Licensing of the potential nuclear-waste repository at Yucca Mountain by the Nuclear Regulatory Commission would require, among other things, demonstrations of the long term usability of the underground facilities. Such a demonstration involves analysis of the mechanical response of the rock to the presence of underground openings and heat-producing waste, which in turn requires data on the mechanical properties of the rock. This document describes the experimental results from a scoping study which led to the development of procedures for performing quality-affecting rock-mechanics experiments on intact rock. The future experiments performed with these procedures will produce information on the time-dependent deformation of welded tuff and represent one aspect of the overall effort to characterize the rheology of the rock mass. 3 refs., 42 figs., 6 tabs.

  16. Source characterization and modeling development for monoenergetic-proton radiography experiments on OMEGA

    SciTech Connect

    Manuel, M. J.-E.; Zylstra, A. B.; Rinderknecht, H. G.; Casey, D. T.; Rosenberg, M. J.; Sinenian, N.; Li, C. K.; Frenje, J. A.; Seguin, F. H.; Petrasso, R. D.

    2012-06-15

    A monoenergetic proton source has been characterized and a modeling tool developed for proton radiography experiments at the OMEGA [T. R. Boehly et al., Opt. Comm. 133, 495 (1997)] laser facility. Multiple diagnostics were fielded to measure global isotropy levels in proton fluence and images of the proton source itself provided information on local uniformity relevant to proton radiography experiments. Global fluence uniformity was assessed by multiple yield diagnostics and deviations were calculated to be {approx}16% and {approx}26% of the mean for DD and D{sup 3}He fusion protons, respectively. From individual fluence images, it was found that the angular frequencies of Greater-Than-Or-Equivalent-To 50 rad{sup -1} contributed less than a few percent to local nonuniformity levels. A model was constructed using the Geant4 [S. Agostinelli et al., Nuc. Inst. Meth. A 506, 250 (2003)] framework to simulate proton radiography experiments. The simulation implements realistic source parameters and various target geometries. The model was benchmarked with the radiographs of cold-matter targets to within experimental accuracy. To validate the use of this code, the cold-matter approximation for the scattering of fusion protons in plasma is discussed using a typical laser-foil experiment as an example case. It is shown that an analytic cold-matter approximation is accurate to within Less-Than-Or-Equivalent-To 10% of the analytic plasma model in the example scenario.

  17. Source characterization and modeling development for monoenergetic-proton radiography experiments on OMEGA

    NASA Astrophysics Data System (ADS)

    Manuel, M. J.-E.; Zylstra, A. B.; Rinderknecht, H. G.; Casey, D. T.; Rosenberg, M. J.; Sinenian, N.; Li, C. K.; Frenje, J. A.; Séguin, F. H.; Petrasso, R. D.

    2012-06-01

    A monoenergetic proton source has been characterized and a modeling tool developed for proton radiography experiments at the OMEGA [T. R. Boehly et al., Opt. Comm. 133, 495 (1997)], 10.1016/S0030-4018(96)00325-2 laser facility. Multiple diagnostics were fielded to measure global isotropy levels in proton fluence and images of the proton source itself provided information on local uniformity relevant to proton radiography experiments. Global fluence uniformity was assessed by multiple yield diagnostics and deviations were calculated to be ˜16% and ˜26% of the mean for DD and D3He fusion protons, respectively. From individual fluence images, it was found that the angular frequencies of ≳50 rad-1 contributed less than a few percent to local nonuniformity levels. A model was constructed using the Geant4 [S. Agostinelli et al., Nuc. Inst. Meth. A 506, 250 (2003)], 10.1016/S0168-9002(03)01368-8 framework to simulate proton radiography experiments. The simulation implements realistic source parameters and various target geometries. The model was benchmarked with the radiographs of cold-matter targets to within experimental accuracy. To validate the use of this code, the cold-matter approximation for the scattering of fusion protons in plasma is discussed using a typical laser-foil experiment as an example case. It is shown that an analytic cold-matter approximation is accurate to within ≲10% of the analytic plasma model in the example scenario.

  18. Characterizing the growth to detonation in PETN and HNS with small-scale PDV cutback experiments

    NASA Astrophysics Data System (ADS)

    Wixom, Ryan; Yarrington, Cole; Knepper, Robert; Tappan, Alexander; Olles, Joseph; Zelenok, Matthew; A-Team

    2015-06-01

    For many decades, cutback experiments have been used to characterize the equation of state and growth to steady detonation in explosive formulations. More recently, embedded gauges have been used to capture the growth to steady detonation in gas-gun impacted samples. Data resulting from these experiments are extremely valuable for parameterizing equation of state and reaction models used in hydrocode simulations. Due to the extremely fast growth to detonation in typical detonator explosives, cutback and embedded gauge experiments are extremely difficult, if not impossible. Using frequency shifted photonic Doppler velocimetry (PDV) we have measured particle velocity histories from explosive films impacted with electrically driven flyers. By varying the sample thickness and impact conditions we were able to capture the growth from inert shock to full detonation pressure within distances as short as 100 μm. These data were used to assess and improve burn-model parameterization and equations of state for simulating shock initiation. Additionally, we discuss details of the experiment and data analysis regarding the most accurate possible determination of the velocity spike.

  19. Design and Characterization of a Neutralized-Transport Experiment for Heavy-Ion Fusion

    SciTech Connect

    Henderson, E; Eylon, S; Roy, P; Yu, S S; Anders, A; Bieniosek, F M; Greenway, W G; Logan, B G; MacGill, R A; Shuman, D B; Vanecek, D L; Waldron, W L; Sharp, W M; Houck, T L; Davidson, R C; Efthimion, P C; Gilson, E P; Sefkow, A B; Welch, D R; Rose, D V; Olson, C L

    2004-05-24

    In heavy-ion inertial-confinement fusion systems, intense beams of ions must be transported from the exit of the final focus magnet system through the fusion chamber to hit millimeter-sized spots on the target. Effective plasma neutralization of intense ion beams in this final transport is essential for a heavy-ion fusion power plant to be economically competitive. The physics of neutralized drift has been studied extensively with particle-in-cell simulations. To provide quantitative comparisons of theoretical predictions with experiment, the Virtual National Laboratory for Heavy Ion Fusion has completed the construction and has begun experimentation with the Neutralized Transport Experiment (NTX). The experiment consists of three main sections, each with its own physics issues. The injector is designed to generate a very high-brightness, space-charge-dominated potassium beam while still allowing variable perveance by a beam aperturing technique. The magnetic-focusing section, consisting of four pulsed magnetic quadrupoles, permits the study of beam tuning, as well as the effects of phase space dilution due to higher-order nonlinear fields. In the final section, a converging ion beam exiting the magnetic section is transported through a drift region with plasma sources for beam neutralization, and the final spot size is measured under various conditions of neutralization. In this paper, we discuss the design and characterization of the three sections in detail and present the first results from the experiment.

  20. Design and characterization of a neutralized-transport experiment for heavy-ion fusion

    SciTech Connect

    Henestroza, E.; Eylon, S.; Roy, P.K.; Yu, S.S.; Anders, A.; Bieniosek, F.M.; Greenway, W.G.; Logan, B.G.; MacGill, R.A.; Shuman, D.B.; Vanecek, D.L.; Waldron, W.L.; Sharp, W.M.; Houck, T.L.; Davidson, R.C.; Efthimion, P.C.; Gilson, E.P.; Sefkow, A.B.; Welch, D.R.; Rose, D.V.; Olson, C.L.

    2004-03-14

    In heavy-ion inertial-confinement fusion systems, intense beams of ions must be transported from the exit of the final focus magnet system through the fusion chamber to hit millimeter-sized spots on the target. Effective plasma neutralization of intense ion beams in this final transport is essential for a heavy-ion fusion power plant to be economically competitive. The physics of neutralized drift has been studied extensively with particle-in-cell simulations. To provide quantitative comparisons of theoretical predictions with experiment, the Virtual National Laboratory for Heavy Ion Fusion has completed the construction and has begun experimentation with the Neutralized Transport Experiment (NTX). The experiment consists of three main sections, each with its own physics issues. The injector is designed to generate a very high-brightness, space-charge-dominated potassium beam while still allowing variable perveance by a beam aperturing technique. The magnetic-focusing section, consisting of four pulsed magnetic quadrupoles, permits the study of beam tuning, as well as the effects of phase space dilution due to higher-order nonlinear fields. In the final section, the converging ion beam exiting the magnetic section is transported through a drift region with plasma sources for beam neutralization, and the final spot size is measured under various conditions of neutralization. In this paper, we discuss the design and characterization of the three sections in detail and present initial results from the experiment.

  1. Direct (13)C-detected NMR experiments for mapping and characterization of hydrogen bonds in RNA.

    PubMed

    Fürtig, Boris; Schnieders, Robbin; Richter, Christian; Zetzsche, Heidi; Keyhani, Sara; Helmling, Christina; Kovacs, Helena; Schwalbe, Harald

    2016-03-01

    In RNA secondary structure determination, it is essential to determine whether a nucleotide is base-paired and not. Base-pairing of nucleotides is mediated by hydrogen bonds. The NMR characterization of hydrogen bonds relies on experiments correlating the NMR resonances of exchangeable protons and can be best performed for structured parts of the RNA, where labile hydrogen atoms are protected from solvent exchange. Functionally important regions in RNA, however, frequently reveal increased dynamic disorder which often leads to NMR signals of exchangeable protons that are broadened beyond (1)H detection. Here, we develop (13)C direct detected experiments to observe all nucleotides in RNA irrespective of whether they are involved in hydrogen bonds or not. Exploiting the self-decoupling of scalar couplings due to the exchange process, the hydrogen bonding behavior of the hydrogen bond donor of each individual nucleotide can be determined. Furthermore, the adaption of HNN-COSY experiments for (13)C direct detection allows correlations of donor-acceptor pairs and the localization of hydrogen-bond acceptor nucleotides. The proposed (13)C direct detected experiments therefore provide information about molecular sites not amenable by conventional proton-detected methods. Such information makes the RNA secondary structure determination by NMR more accurate and helps to validate secondary structure predictions based on bioinformatics. PMID:26852414

  2. Probe experiment characterizing 30-MHz radio wave scatter in the high-latitude ionosphere

    NASA Astrophysics Data System (ADS)

    Nishino, M.; Gorokhov, N.; Tanaka, Y.; Yamagishi, H.; Hansen, T.

    1999-07-01

    A probe experiment, consisting of radio links between a common 30-MHz transmitter located at Murmansk, Russia, and two receivers used as the imaging riometer (two-dimensional 64 multiple-beam antenna) located at Ny Ålesund, Svalbard, and Tjornes, Iceland, was carried out to characterize wave scatter in the high-latitude ionosphere. They are nearly aligned with and perpendicular to the geomagnetic meridian, respectively. In experiments conducted in March-April 1994, the 30-MHz probe signals were identified at nighttime more frequently than during the day at both receiver stations during periods of increased geomagnetic activity near the path midpoints, indicating that a relationship between the propagation path and the location of the auroral oval controls signal identification. For the nighttime propagation paths within or crossing through the auroral oval, duty cycles of the probe signals were roughly correlated with increases in geomagnetic activity. Their arrival directions showed a spread with a dominant power on the low elevation and a normal distribution in azimuth. These results indicate that the probe signals are characterized as nonmeteoric "auroral E" scatter caused by irregular, large-scale profiles of electron density enhancements at the lower edge of the ionosphere. However, on 2 days of weak geomagnetic activity, strong probe signals with bursty behavior were identified by an extremely high duty cycle (˜98%) for the nighttime meridian path only, and their arrival directions showed an isotropic spread in azimuth. Such nonmeteoric probe signals are characterized as "coherent" scatter caused by small-scale (˜5 m) field-aligned irregularities in electron density in the E region ionosphere, related to "sporadic E" occurrence.

  3. White light interferometry for quantitative surface characterization in ion sputtering experiments.

    SciTech Connect

    Baryshev, S. V.; Zinovev, A. V.; Tripa, C. E.; Erck, R. A.; Veryovkin, I. V.

    2012-07-01

    White light interferometry (WLI) can be used to obtain surface morphology information on dimensional scale of millimeters with lateral resolution as good as {approx}1 {micro}m and depth resolution down to 1 nm. By performing true three-dimensional imaging of sample surfaces, the WLI technique enables accurate quantitative characterization of the geometry of surface features and compares favorably to scanning electron and atomic force microscopies by avoiding some of their drawbacks. In this paper, results of using the WLI imaging technique to characterize the products of ion sputtering experiments are reported. With a few figures, several example applications of the WLI method are illustrated when used for (i) sputtering yield measurements and time-to-depth conversion, (ii) optimizing ion beam current density profiles, the shapes of sputtered craters, and multiple ion beam superposition and (iii) quantitative characterization of surfaces processed with ions. In particular, for sputter depth profiling experiments of {sup 25}Mg, {sup 44}Ca and {sup 53}Cr ion implants in Si (implantation energy of 1 keV per nucleon), the depth calibration of the measured depth profile curves determined by the WLI method appeared to be self-consistent with TRIM simulations for such projectile-matrix systems. In addition, high depth resolution of the WLI method is demonstrated for a case of a Genesis solar wind Si collector surface processed by gas cluster ion beam: a 12.5 nm layer was removed from the processed surface, while the transition length between the processed and untreated areas was 150 {micro}m.

  4. Characterize Behaviour of Emerging Pollutants in Artificial Recharge: Column Experiments - Experiment Design and Results of Preliminary Tests

    NASA Astrophysics Data System (ADS)

    Wang, H.; Carrera, J.; Ayora, C.; Licha, T.

    2012-04-01

    Emerging pollutants (EPs) have been detected in water resources as a result of human activities in recent years. They include pharmaceuticals, personal care products, dioxins, flame retardants, etc. They are a source of concern because many of them are resistant to conventional water treatment, and they are harmful to human health, even in low concentrations. Generally, this study aims to characterize the behaviour of emerging pollutants in reclaimed water in column experiments which simulates artificial recharge. One column set includes three parts: influent, reactive layer column (RLC) and aquifer column (AC). The main influent is decided to be Secondary Effluent (SE) of El Prat Wastewater Treatment Plant, Barcelona. The flow rate of the column experiment is 0.9-1.5 mL/min. the residence time of RLC is designed to be about 1 day and 30-40 days for AC. Both columns are made of stainless steel. Reactive layer column (DI 10cm * L55cm) is named after the filling material which is a mixture of organic substrate, clay and goethite. One purpose of the application of the mixture is to increase dissolve organic carbon (DOC). Leaching test in batchs and columns has been done to select proper organic substrate. As a result, compost was selected due to its long lasting of releasing organic matter (OM). The other purpose of the application of the mixture is to enhance adsorption of EPs. Partition coefficients (Kow) of EPs indicate the ability of adsorption to OM. EPs with logKow>2 could be adsorbed to OM, like Ibuprofen, Bezafibrate and Diclofenac. Moreover, some of EPs are charged in the solution with pH=7, according to its acid dissociation constant (Ka). Positively charged EPs, for example Atenolol, could adsorb to clay. In the opposite, negatively charged EPs, for example Gemfibrozil, could adsorb to goethite. Aquifer column (DI 35cm * L1.5m) is to simulate the processes taking place in aquifer in artificial recharge. The filling of AC has two parts: silica sand and

  5. Pre-Flight Characterization of Samples for the MISSE-7 Spacesuit Fabric Exposure Experiment

    NASA Technical Reports Server (NTRS)

    Gaier, James R.; McCue, Terry R.; Clark, Gregory W.; Rogers, Kerry J.; Mengesu, Tsega

    2009-01-01

    A series of six sample spacesuit pressure garment assembly (PGA) fabric samples were prepared for the Materials International Space Station Experiment 7 (MISSE-7) flight experiment to test the effects of damage by lunar dust on the susceptibility of the fabrics to radiation damage. These included pristine Apollo-era fluorinated ethylene-propylene (FEP) fabric, Apollo-era FEP fabric that had been abraded with JSC-1A lunar simulant, and a piece of Alan Bean s Apollo 12 PGA sectioned from near the left knee. Also included was a sample of pristine orthofabric, and orthofabric that had been abraded to two different levels with JSC-1A. The samples were characterized using optical microscopy, field emission scanning electron microscopy, and atomic force microscopy. Two sets of six samples were then loaded in space environment exposure hardware, one of which was stored as control samples. The other set was affixed to the MISSE-7 experiment package, and will be mounted on the International Space Station, and exposed to the wake-side low Earth orbit environment. It will be retrieved after an exposure of approximately 12 months, and returned for post flight analysis.

  6. Experiment to Characterize Aircraft Volatile Aerosol and Trace-Species Emissions (EXCAVATE)

    NASA Technical Reports Server (NTRS)

    Anderson, B. E.; Branham, H.-S.; Hudgins, C. H.; Plant, J. V.; Ballenthin, J. O.; Miller, T. M.; Viggiano, A. A.; Blake, D. R.; Boudries, H.; Canagaratna, M.

    2005-01-01

    The Experiment to Characterize Aircraft Volatile and Trace Species Emissions (EXCAVATE) was conducted at Langley Research Center (LaRC) in January 2002 and focused upon assaying the production of aerosols and aerosol precursors by a modern commercial aircraft, the Langley B757, during ground-based operation. Remaining uncertainty in the postcombustion fate of jet fuel sulfur contaminants, the need for data to test new theories of particle formation and growth within engine exhaust plumes, and the need for observations to develop air quality models for predicting pollution levels in airport terminal areas were the primary factors motivating the experiment. NASA's Atmospheric Effects of Aviation Project (AEAP) and the Ultra Effect Engine Technology (UEET) Program sponsored the experiment which had the specific objectives of determining ion densities; the fraction of fuel S converted from S(IV) to S(VI); the concentration and speciation of volatile aerosols and black carbon; and gas-phase concentrations of long-chain hydrocarbon and PAH species, all as functions of engine power, fuel composition, and plume age.

  7. Syntheses and Characterization of Ruthenium(II) Tetrakis(pyridine)complexes: An Advanced Coordination Chemistry Experiment or Mini-Project

    ERIC Educational Resources Information Center

    Coe, Benjamin J.

    2004-01-01

    An experiment for third-year undergraduate a student is designed which provides synthetic experience and qualitative interpretation of the spectroscopic properties of the ruthenium complexes. It involves the syntheses and characterization of several coordination complexes of ruthenium, the element found directly beneath iron in the middle of the…

  8. CO2-induced dissolution of low permeability carbonates. Part I: Characterization and experiments

    NASA Astrophysics Data System (ADS)

    Smith, Megan M.; Sholokhova, Yelena; Hao, Yue; Carroll, Susan A.

    2013-12-01

    The effect of elevated dissolved CO2 concentrations on compositionally and structurally distinct carbonate sample cores from the Weyburn-Midale CO2-enhanced oil recovery and storage site (Canada) was measured from analysis of 3-D sample characterization and fluid chemistry data from core-flood experiments. Experimental conditions (60 °C; 24.8 MPa confining pressure) and brine composition were chosen to mimic in situ reservoir conditions. Mineralogy and pore space distributions within the eight individual cores were characterized with X-ray computed microtomography and scanning electron microscopy both before and after exposure to brine with 0.5 ⩽ pCO2 ⩽ 3 MPa, while solution chemistry and differential fluid pressures were monitored during experiments. Our experimental study aimed to quantify the relationship between fluid flow, heterogeneity, and reaction specific to carbon storage at the Weyburn-Midale field by integrating characterization imaging, pressure data, and solution chemistry. Through the use of non-invasive microtomographic imaging, a variety of dissolution behaviors were observed, with variable effects on the evolution of solution chemistry and permeability as a result of heterogeneity within these two relatively low permeability carbonate samples. Similar-sized, evenly distributed pores, and steadily advancing dissolution fronts suggested that uniform flow velocities were maintained throughout the duration of the higher permeability “Marly” dolostone core experiments. The development of unstable dissolution fronts and fast pathways occurred in the “Vuggy” sample experiments when fluid velocities varied widely within the sample (as a result of increased pore structure heterogeneity). The overall effect of fast pathway development was to increase bulk permeability values by several orders of magnitude, allowing CO2-acidified fluids to travel through the cores largely unmodified by carbonate mineral reaction, as indicated by a lack of change

  9. Preliminary characterization of materials for a reactive transport model validation experiment

    SciTech Connect

    Siegel, M.D.; Ward, D.B.; Cheng, W.C.; Bryant, C.; Chocas, C.S.; Reynolds, C.G.

    1993-03-01

    The geochemical properties of a porous sand and several tracers (Ni, Br, and Li) have been characterized for use in a caisson experiment designed to validate sorption models used in models of inactive transport. The surfaces of the sand grains have been examined by a combination of techniques including potentiometric titration, acid leaching, optical microscopy, and scanning electron microscopy with energy-dispersive spectroscopy. The surface studies indicate the presence of small amounts of carbonate, kaolinite and iron-oxyhydroxides. Adsorption of nickel, lithium and bromide by the sand was measured using batch techniques. Bromide was not sorbed by the sand. A linear (K{sub d}) or an isotherm sorption model may adequately describe transport of Li; however, a model describing the changes of pH and the concentrations of other solution species as a function of time and position within the caisson and the concomitant effects on Ni sorption may be required for accurate predictions of nickel transport.

  10. Walker Branch Throughfall Displacement Experiment Data Report: Site Characterization, System Performance, Weather, Species Composition, and Growth

    SciTech Connect

    Hanson, P.J.

    2001-09-04

    This numeric data package provides data sets, and accompanying documentation, on site characterization, system performance, weather, species composition, and growth for the Throughfall Displacement Experiment, which was established in the Walker Branch Watershed of East Tennessee to provide data on the responses of forests to altered precipitation regimes. The specific data sets include soil water content and potential, coarse fraction of the soil profile, litter layer temperature, soil temperature, monthly weather, daily weather, hourly weather, species composition of trees and saplings, mature tree and sapling annual growth, and relative leaf area index. Fortran and SAS{trademark} access codes are provided to read the ASCII data files. The data files and this documentation are available without charge on a variety of media and via the Internet from the Carbon Dioxide Information Analysis Center (CDIAC).

  11. Characterization of plasma and laser conditions for single hot spot interaction experiments

    SciTech Connect

    Montgomery, D.S.; Johnson, R.P.; Cobble, J.A.; Fernandez, J.C.; Lindman, E.L.; Rose, H.A.; Estabrook, K.G.

    1998-11-01

    The LANL TRIDENT laser system is being used for fundamental experiments which study the interaction of self-focusing, stimulated Raman scattering (SRS) and stimulated Brillouin scattering (SBS) in a single (diffraction limited) laser hot spot in order to better understand the coupling between these plasma instabilities. The diffraction limited beam mimics a single hot spot found in speckle distributions that are typical of random or kinoform phase plate (RPP or KPP) smoothing. A long scale length, hot plasma ({approximately} 1 mm, {approximately} 0.5 keV) is created by a separate heater beam, and the single hot spot beam is used to drive parametric instabilities. The focal plane distribution and wavefront of the single hot spot beam are characterized, and the intensity of the single hot spot can be varied between 10{sup 14}--10{sup 16} W/cm{sup 2}. The plasma density, temperature, and flow profiles are measured using gated imaging spectroscopy of collective Thomson scattering. Results of the laser and plasma characterization, and initial results of backscattered SRS, SBS, and beam steering in a flowing plasma are presented.

  12. Oscillatory Hydraulic Tomography for NAPL Source Zone Characterization: Sandbox Experiment Demonstration

    NASA Astrophysics Data System (ADS)

    Cardiff, M. A.; Zhou, Y.

    2015-12-01

    Characterizing the distribution and extent of NAPL contamination is an important step in determining appropriate remedial actions. NAPL has a complex mode of transportation in the heterogeneous subsurface domain, which results in difficulties for cleaning up contaminated sites. Here, we use sandbox experiments to demonstrate the effectiveness of Oscillatory Hydraulic Tomography (OHT) for NAPL source zone characterization. In a saturated soil fluid system, the effective hydraulic conductivity (K) is dependent on the soil properties, fluid density, and fluid viscosity. By taking advantage of the differences of fluid properties before and after NAPL intrusion, we can estimate the NAPL source zone migration throughout time by imaging changes in effective K. Using OHT testing, we can derive the K heterogeneities before, during and after NAPL intrusion. NAPL source zone can be located by subtracting the background K from the K tomogram after NAPL intrusion. This approach can avoid mass extraction and injection that occurs in traditional hydraulic tomography approaches while obtain a good estimation of subsurface K heterogeneity and NAPL migration. We believe this method is more cost effective and efficient for field remediation applications.

  13. Post-Flight Characterization of Samples for the MISSE-7 Spacesuit Fabric Exposure Experiment

    NASA Technical Reports Server (NTRS)

    Gaier, James R.; Waters, Deborah L.; Jaworski, Donald A.; McCue, Terry R.; Folz, Angela; Baldwin, Sammantha; Clark, Gregory W.; Batman, Brittany; Bruce, John

    2012-01-01

    Six samples of pristine and dust-abraded outer layer spacesuit fabrics were included in the Materials International Space Station Experiment-7, in which they were exposed to the wake side low Earth orbit environment (LEO) on the International Space Station (ISS) for 18 months in order to determine whether abrasion by lunar dust increases radiation degradation. The fabric samples were characterized using optical microscopy, field emission scanning electron microscopy, and tensile testing before and after exposure on the ISS. Comparison of pre- and post-flight characterizations showed that wake side LEO environment darkened and reddened all six fabrics, increasing their integrated solar absorptance by 7 to 38 percent. There was a decrease in the ultimate tensile strength and elongation to failure of lunar dust abraded Apollo spacesuit fibers by a factor of four and increased the elastic modulus by a factor of two. The severity of the degradation of the fabric samples over this short exposure time demonstrates the necessity to find ways to prevent or mitigate radiation damage to spacesuits when planning extended missions to the Moon.

  14. Using radar tomography, tracer experiments and hydraulic data to characterize fractured rock flow systems

    NASA Astrophysics Data System (ADS)

    Day-Lewis, Frederick David

    Among the most pressing problems in hydrogeology is describing heterogeneity in fractured rock, where data are typically local and sparse, and permeability varies by orders of magnitude over short distances. This dissertation presents new approaches to characterize fractured rock groundwater flow systems using cross-well radar, tracer, and hydraulic experiments. The methods are demonstrated using data from the U.S. Geological Survey Fractured Rock Hydrology Research Site near Mirror Lake, New Hampshire. One underutilized source of information in characterization of fractured rock is hydraulic connection data. Wells connected by a high-permeability fracture zone tend to exhibit similar hydraulic responses during pumping or drilling. A simulated-annealing algorithm is presented to condition geostatistical simulations to inferred connections. The method is used to generate 3-D realizations of fracture-zone geometry at the Mirror Lake Site. Results indicate the likely extents of specific zones. Flow models based on realizations are calibrated to hydraulic data to estimate the hydraulic parameters of the fracture zones and surrounding bedrock. Another innovative source of information for characterization is time-lapse difference-attenuation radar tomography, which has been used to monitor the migration of electrically conductive saline tracers. A sequential-inversion methodology is presented and demonstrated for a synthetic example. The method uses space-time parameterization and regularization to account for changes in concentration that occur quickly relative to the collection of radar data. The time-lapse tomographic inversion method is applied to data from the Mirror Lake Site. Difference-attenuation tomography indicates the timing and spatial distribution of tracer transport in three planes that form a triangular prism. Tracer migration is focused along a preferential pathway. Comparison of the time-series of tomograms with the outlet tracer data suggests that much

  15. Integrated Modeling and Experiments to Characterize Coupled Thermo-hydro-geomechanical-chemical processes in Hydraulic Fracturing

    NASA Astrophysics Data System (ADS)

    Viswanathan, H. S.; Carey, J. W.; Karra, S.; Porter, M. L.; Rougier, E.; Kang, Q.; Makedonska, N.; Hyman, J.; Jimenez Martinez, J.; Frash, L.; Chen, L.

    2015-12-01

    Hydraulic fracturing phenomena involve fluid-solid interactions embedded within coupled thermo-hydro-mechanical-chemical (THMC) processes over scales from microns to tens of meters. Feedbacks between processes result in complex dynamics that must be unraveled if one is to predict and, in the case of unconventional resources, facilitate fracture propagation, fluid flow, and interfacial transport processes. The proposed work is part of a broader class of complex systems involving coupled fluid flow and fractures that are critical to subsurface energy issues, such as shale oil, geothermal, carbon sequestration, and nuclear waste disposal. We use unique LANL microfluidic and triaxial core flood experiments integrated with state-of-the-art numerical simulation to reveal the fundamental dynamics of fracture-fluid interactions to characterize the key coupled processes that impact hydrocarbon production. We are also comparing CO2-based fracturing and aqueous fluids to enhance production, greatly reduce waste water, while simultaneously sequestering CO2. We will show pore, core and reservoir scale simulations/experiments that investigate the contolling mechanisms that control hydrocarbon production.

  16. New Laboratory-Based Satellite Impact Experiments for Breakup Fragment Characterization

    NASA Astrophysics Data System (ADS)

    Liou, J.-C.

    A consortium consisting of the NASA Orbital Debris Program Office, U.S. Air Force’s Space and Missile Systems Center, The Aerospace Corporation, and University of Florida is planning a series of hypervelocity impact experiments on mockup targets at the U.S. Air Force’s Arnold Engineering Development Complex (AEDC) in early 2014. The target for the first experiment resembles a rocket upper stage whereas the target for the second experiment represents a typical 60-cm/50-kg class payload that incorporates modern spacecraft materials and components as well as exterior wrap of multi-layer insulation and three solar panels. The projectile is designed with the maximum mass that AEDC’s Range G two-stage light gas gun can accelerate to an impact speed of 7 km/sec. The impact energy is expected to be close to 15 MJ to ensure catastrophic destruction of the target after the impact. Low density foam panels are installed inside the target chamber to slow down and soft-catch the fragments for post-impact processing. Diagnostic instruments, such as x-ray and high speed optical cameras, will also be used to record the breakup process. The main goal of this “DebriSat” project is to characterize the physical properties, including size, mass, shape, and density distributions, of orbital debris that would be generated by a hypervelocity collision involving an upper stage or a modern satellite in the low Earth orbit environment. In addition, representative fragments will be selected for laboratory optical and radar measurements to allow for better interpretation of data obtained by telescope and radar observations. This paper will provide a preliminary report of the impact results and the plans to process, measure, and analyze the fragments.

  17. New Laboratory-Based Satellite Impact Experiments for Breakup Fragment Characterization

    NASA Technical Reports Server (NTRS)

    Liou, J.-C.; Fitz-Coy, N.; Dikova, R.; Wilson, M.; Huynh, T.; Sorge, M.; Sheaffer, P.; Opiela, J.; Cowardin, H.; Krisko, P.; Rushing, R.; Nolen, M.; Roebuck, B.

    2014-01-01

    A consortium consisting of the NASA Orbital Debris Program Office, U.S. Air Force's Space and Missile Systems Center, the Aerospace Corporation, and University of Florida is planning a series of hypervelocity impact experiments on mockup targets at the U.S. Air Force's Arnold Engineering Development Complex (AEDC) in early 2014. The target for the first experiment resembles a rocket upper stage whereas the target for the second experiment represents a typical 60-cm/50-kg class payload that incorporates modern spacecraft materials and components as well as exterior wrap of multi-layer insulation and three solar panels. The projectile is designed with the maximum mass that AEDC's Range G two-stage light gas gun can accelerate to an impact speed of 7 km/sec. The impact energy is expected to be close to 15 MJ to ensure catastrophic destruction of the target after the impact. Low density foam panels are installed inside the target chamber to slow down and soft-catch the fragments for post-impact processing. Diagnostic instruments, such as x-ray and high speed optical cameras, will also be used to record the breakup process. The main goal of this "DebriSat" project is to characterize the physical properties, including size, mass, shape, and density distributions, of orbital debris that would be generated by a hypervelocity collision involving an upper stage or a modern satellite in the low Earth orbit environment. In addition, representative fragments will be selected for laboratory optical and radar measurements to allow for better interpretation of data obtained by telescope and radar observations. This paper will provide a preliminary report of the impact results and the plans to process, measure, and analyze the fragments.

  18. Characterization of blocks impacts from acoustic emissions: insights from laboratory experiments

    NASA Astrophysics Data System (ADS)

    Farin, Maxime; Mangeney, Anne; de Rosny, Julien; Toussaint, Renaud; Shapiro, Nikolaï

    2014-05-01

    Rockfalls, debris flows and rock avalanches represent a major natural hazard for the population in mountainous, volcanic and coastal areas but their direct observation on the field is very dangerous. Recent studies showed that gravitational instabilities can be detected and characterized (volume, duration,...) thanks to the seismic signal they generate. In an avalanche, individual block bouncing and rolling on the ground are expected to generated signals of higher frequencies than the main flow spreading. The identification of the time/frequency signature of individual blocks in the recorded signal remains however difficult. Laboratory experiments were conducted to investigate the acoustic signature of diverse simple sources corresponding to grains falling over thin plates of plexiglas and glass and over rock blocks. The elastic energy emitted by a single bouncing bead into the support was first quantitatively estimated and compared to the potential energy of fall and to the potential energy change during the shock. We obtained simple scaling laws relating the impactor characteristics (size, height of fall, material,...) to the elastic energy and spectral content. Next, we consider the collapse of granular columns made of steel spherical beads onto hard substrates. Initially, these columns were held by a magnetic field allowing to suppress suddenly the cohesion between the beads, and thus to minimize friction effects that would arise from side walls. We varied systematically the column volume, the column aspect ratio (height over length) and the grain size. This is shown to affect the signal envelope and frequency content. In the experiments, accelerometers (1 Hz to 56 kHz) were used to record the signals in a wide frequency range. The experiments were also monitored optically using fast cameras. Eventually, we looked at what types of features in the signal are affected by individual impacts, rolling of beads or by the large scale geometry of the avalanche.

  19. Characterization of blocks impacts from elastic waves: insights from laboratory experiments

    NASA Astrophysics Data System (ADS)

    Farin, M.; Mangeney, A.; Toussaint, R.; De Rosny, J.; Shapiro, N.

    2013-12-01

    Rockfalls, debris flows and rock avalanches constitute a major natural hazard for the population in mountainous, volcanic and coastal areas but their direct observation on the field is very dangerous. Recent studies showed that gravitational instabilities can be detected and characterized (volume, duration,...) thanks to the seismic signal they generate. In an avalanche, individual block bouncing and rolling on the ground are expected to generated signals of higher frequencies than the main flow spreading. The identification of the time/frequency signature of individual blocks in the recorded signal remains however difficult. Laboratory experiments were conducted to investigate the acoustic signature of diverse simple sources corresponding to grains falling over thin plates of plexiglas and rock blocks. The elastic energy emitted by a single bouncing steel bead into the support was first quantitatively estimated and compared to the potential energy of fall and to the potential energy change during the shock. Next, we consider the collapse of granular columns made of steel spherical beads onto hard substrates. Initially, these columns were held by a magnetic field allowing to suppress suddenly the cohesion between the beads, and thus to minimize friction effects that would arise from side walls. We varied systematically the column volume, the column aspect ratio (height over length) and the grain size. This is shown to affect the signal envelope and frequency content. In the experiments, two types of acoustic sensors were used to record the signals in a wide frequency range: accelerometers (1 Hz to 56 kHz) and piezoelectric sensors (100 kHz to 1 MHz). The experiments were also monitored optically using fast cameras. We developed a technique to use quantitatively both types of sensors to evaluate the elastic energy emitted by the sources. Eventually, we looked at what types of features in the signal are affected by individual shocks or by the large scale geometry of

  20. Characterization of stony soils' hydraulic conductivity using laboratory and numerical experiments

    NASA Astrophysics Data System (ADS)

    Beckers, Eléonore; Pichault, Mathieu; Pansak, Wanwisa; Degré, Aurore; Garré, Sarah

    2016-08-01

    Determining soil hydraulic properties is of major concern in various fields of study. Although stony soils are widespread across the globe, most studies deal with gravel-free soils, so that the literature describing the impact of stones on the hydraulic conductivity of a soil is still rather scarce. Most frequently, models characterizing the saturated hydraulic conductivity of stony soils assume that the only effect of rock fragments is to reduce the volume available for water flow, and therefore they predict a decrease in hydraulic conductivity with an increasing stoniness. The objective of this study is to assess the effect of rock fragments on the saturated and unsaturated hydraulic conductivity. This was done by means of laboratory experiments and numerical simulations involving different amounts and types of coarse fragments. We compared our results with values predicted by the aforementioned predictive models. Our study suggests that it might be ill-founded to consider that stones only reduce the volume available for water flow. We pointed out several factors of the saturated hydraulic conductivity of stony soils that are not considered by these models. On the one hand, the shape and the size of inclusions may substantially affect the hydraulic conductivity. On the other hand, laboratory experiments show that an increasing stone content can counteract and even overcome the effect of a reduced volume in some cases: we observed an increase in saturated hydraulic conductivity with volume of inclusions. These differences are mainly important near to saturation. However, comparison of results from predictive models and our experiments in unsaturated conditions shows that models and data agree on a decrease in hydraulic conductivity with stone content, even though the experimental conditions did not allow testing for stone contents higher than 20 %.

  1. Characterization of blocks impacts from seismic signal: insights from laboratory experiments

    NASA Astrophysics Data System (ADS)

    Farin, M.; Mangeney, A.; Toussaint, R.; de Rosny, J.; Sainte-Marie, J.; Shapiro, N.

    2014-12-01

    Rockfalls, debris flows and rock avalanches represent a major natural hazard for the population in mountainous, volcanic and coastal areas but their direct observation on the field is very difficult. Recent field studies showed that gravitational instabilities can be detected, localized and characterized thanks to the seismic signal they generate. Therefore, a burning challenge for risks assessment related to these events is to obtain quantiative informations on the characteristics of the rockfalls (mass, speed, extension,...) from the properties of the signal (seismic energy, frequencies,...). Using a theoretical model of viscoelastic impact of a sphere on a plane, we develop analytical scaling laws relating the energy radiated in elastic waves, the energy dissipated in viscoelasticity during the impact and the frequencies of the generated acoustic signal to the mass m and the impact speed Vz of the sphere and to the elastic parameters of the involved materials. The elastic energy is shown to vary as m5/3Vz11/5 on plates and as mVz13/5 on blocks, regardless of the elastic parameters. The energy dissipated in viscoelasticity does not depend on the support thickness and varies as m2/3Vz11/5. The mean frequency of the generated signal is inversely proportional to the impact duration. Then, we conduct simple laboratory experiments that consist in dropping spherical beads of different size and materials and small gravels on thin plates of glass and Plexiglass and rock blocks. The elastic energy emitted by an impact on the supports is first quantitatively estimated and compared to the potential energy of fall and to the potential energy change during the shock. We observe a quantitative agreement between experimental data and the analytical scaling laws, even when we use small gravels instead of spherical beads as impactors. These experiments allows to valid the theoretical model and to establish the energy budget of an impact. In the experiments, piezoelectric

  2. Detailed characterizations of a Comparative Reactivity Method (CRM) instrument: experiments vs. modelling

    NASA Astrophysics Data System (ADS)

    Michoud, V.; Hansen, R. F.; Locoge, N.; Stevens, P. S.; Dusanter, S.

    2015-04-01

    simple chemical mechanism, taking into account the inorganic chemistry from IUPAC 2001 and a simple organic chemistry scheme including only a generic RO2 compounds for all oxidized organic trace gases; and (2) a more exhaustive chemical mechanism, based on the Master Chemical Mechanism (MCM), including the chemistry of the different trace gases used during laboratory experiments. Both mechanisms take into account self- and cross-reactions of radical species. The simulations using these mechanisms allow reproducing the magnitude of the corrections needed to account for NO interferences and a deviation from pseudo first-order kinetics, as well as their dependence on the Pyrrole-to-OH ratio and on bimolecular reaction rate constants of trace gases. The reasonable agreement found between laboratory experiments and model simulations gives confidence in the parameterizations proposed to correct the Total OH reactivity measured by CRM. However, it must be noted that the parameterizations presented in this paper are suitable for the CRM instrument used during the laboratory characterization and may be not appropriate for other CRM instruments, even if similar behaviours should be observed. It is therefore recommended that each group characterizes its own instrument following the recommendations given in this study. Finally, the assessment of the limit of detection and total uncertainties is discussed and an example of field deployment of this CRM instrument is presented.

  3. Entamoeba Clone-Recognition Experiments: Morphometrics, Aggregative Behavior, and Cell-Signaling Characterization.

    PubMed

    Espinosa, Avelina; Paz-Y-Miño-C, Guillermo; Hackey, Meagan; Rutherford, Scott

    2016-05-01

    Studies on clone- and kin-discrimination in protists have proliferated during the past decade. We report clone-recognition experiments in seven Entamoeba lineages (E. invadens IP-1, E. invadens VK-1:NS, E. terrapinae, E. moshkovskii Laredo, E. moshkovskii Snake, E. histolytica HM-1:IMSS and E. dispar). First, we characterized morphometrically each clone (length, width, and cell-surface area) and documented how they differed statistically from one another (as per single-variable or canonical-discriminant analyses). Second, we demonstrated that amebas themselves could discriminate self (clone) from different (themselves vs. other clones). In mix-cell-line cultures between closely-related (E. invadens IP-1 vs. E. invadens VK-1:NS) or distant-phylogenetic clones (E. terrapinae vs. E. moshkovskii Laredo), amebas consistently aggregated with same-clone members. Third, we identified six putative cell-signals secreted by the amebas (RasGap/Ankyrin, coronin-WD40, actin, protein kinases, heat shock 70, and ubiquitin) and which known functions in Entamoeba spp. included: cell proliferation, cell adhesion, cell movement, and stress-induced encystation. To our knowledge, this is the first multi-clone characterization of Entamoeba spp. morphometrics, aggregative behavior, and cell-signaling secretion in the context of clone-recognition. Protists allow us to study cell-cell recognition from ecological and evolutionary perspectives. Modern protistan lineages can be central to studies about the origins and evolution of multicellularity. PMID:26990199

  4. Petrologic and geochemical characterization of the Bullfrog Member of the Crater Flat Tuff: outcrop samples used in waste package experiments

    SciTech Connect

    Knauss, K.G.

    1983-09-01

    In support of the Waste Package Task within the Nevada Nuclear Waste Storage Investigation (NNWSI), experiments on hydrothermal rock/water interaction, corrosion, thermomechanics, and geochemical modeling calculations are being conducted. All of these activities require characterization of the initial bulk composition, mineralogy, and individual phase geochemistry of the potential repository host rock. This report summarizes the characterization done on samples of the Bullfrog Member of the Crater Flat Tuff (Tcfb) used for Waste Package experimental programs. 11 references, 17 figures, 3 tables.

  5. From experiment to design -- Fault characterization and detection in parallel computer systems using computational accelerators

    NASA Astrophysics Data System (ADS)

    Yim, Keun Soo

    This dissertation summarizes experimental validation and co-design studies conducted to optimize the fault detection capabilities and overheads in hybrid computer systems (e.g., using CPUs and Graphics Processing Units, or GPUs), and consequently to improve the scalability of parallel computer systems using computational accelerators. The experimental validation studies were conducted to help us understand the failure characteristics of CPU-GPU hybrid computer systems under various types of hardware faults. The main characterization targets were faults that are difficult to detect and/or recover from, e.g., faults that cause long latency failures (Ch. 3), faults in dynamically allocated resources (Ch. 4), faults in GPUs (Ch. 5), faults in MPI programs (Ch. 6), and microarchitecture-level faults with specific timing features (Ch. 7). The co-design studies were based on the characterization results. One of the co-designed systems has a set of source-to-source translators that customize and strategically place error detectors in the source code of target GPU programs (Ch. 5). Another co-designed system uses an extension card to learn the normal behavioral and semantic execution patterns of message-passing processes executing on CPUs, and to detect abnormal behaviors of those parallel processes (Ch. 6). The third co-designed system is a co-processor that has a set of new instructions in order to support software-implemented fault detection techniques (Ch. 7). The work described in this dissertation gains more importance because heterogeneous processors have become an essential component of state-of-the-art supercomputers. GPUs were used in three of the five fastest supercomputers that were operating in 2011. Our work included comprehensive fault characterization studies in CPU-GPU hybrid computers. In CPUs, we monitored the target systems for a long period of time after injecting faults (a temporally comprehensive experiment), and injected faults into various types of

  6. Site Characterization of the Source Physics Experiment Phase II Location Using Seismic Reflection Data

    NASA Astrophysics Data System (ADS)

    Sexton, E. A.; Snelson, C. M.; Chipman, V.; Emer, D. F.; White, R. L.; Emmitt, R.; Wright, A. A.; Drellack, S.; Huckins-Gang, H.; Mercadante, J.; Floyd, M.; McGowin, C.; Cothrun, C.; Bonal, N.

    2013-12-01

    An objective of the Source Physics Experiment (SPE) is to identify low-yield nuclear explosions from a regional distance. Low-yield nuclear explosions can often be difficult to discriminate among the clutter of natural and man-made explosive events (e.g., earthquakes and mine blasts). The SPE is broken into three phases. Phase I has provided the first of the physics-based data to test the empirical models that have been used to discriminate nuclear events. The Phase I series of tests were placed within a highly fractured granite body. The evolution of the project has led to development of Phase II, to be placed within the opposite end member of geology, an alluvium environment, thereby increasing the database of waveforms to build upon in the discrimination models. Both the granite and alluvium sites have hosted nearby nuclear tests, which provide comparisons for the chemical test data. Phase III of the SPE is yet to be determined. For Phase II of the experiment, characterization of the location is required to develop the geologic/geophysical models for the execution of the experiment. Criteria for the location are alluvium thickness of approximately 170 m and a water table below 170 m; minimal fracturing would be ideal. A P-wave mini-vibroseis survey was conducted at a potential site in alluvium to map out the subsurface geology. The seismic reflection profile consisted of 168 geophone stations, spaced 5 m apart. The mini-vibe was a 7,000-lb peak-force source, starting 57.5 m off the north end of the profile and ending 57.5 m past the southern-most geophone. The length of the profile was 835 m. The source points were placed every 5 m, equally spaced between geophones to reduce clipping. The vibroseis sweep was from 20 Hz down to 180 Hz over 8 seconds, and four sweeps were stacked at each shot location. The shot gathers show high signal-to-noise ratios with clear first arrivals across the entire spread and the suggestion of some shallow reflectors. The data were

  7. Aquifer imaging with pressure waves—Evaluation of low-impact characterization through sandbox experiments

    NASA Astrophysics Data System (ADS)

    Zhou, YaoQuan; Lim, David; Cupola, Fausto; Cardiff, Michael

    2016-03-01

    Understanding the detailed spatial variation of hydraulic properties in the subsurface has been the subject of intensive research over the past three decades. A recently developed approach to characterize subsurface properties is hydraulic tomography, in which a series of pumping tests are jointly inverted using a heterogeneous numerical model. Recently, Cardiff et al. (2013) proposed a modified tomography approach named Oscillatory Hydraulic Tomography (OHT), in which periodic pumping signals of different frequencies serve as the aquifer stimulation, and pressure responses are recorded at observation locations for tomographic analysis. Its key advantages over traditional hydraulic tomography are that: (1) there is no net injection or extraction of water, and (2) the impulse (an oscillatory signal of known frequency) is easily extracted from noisy data. However, OHT has only been evaluated through numerical experiments to date. In this work, we evaluate OHT performance by attempting to image known heterogeneities in a synthetic aquifer. An instrumented laboratory sandbox is filled with material of known hydraulic properties, and we measure aquifer responses due to oscillatory pumping stimulations at periods of 2, 5, 10, and 20 s. Pressure oscillation time series are processed through Fourier Transforms and inverted tomographically to obtain estimates of aquifer heterogeneity, using a fast, steady-periodic groundwater flow model. We show that OHT is able to provide robust estimates of aquifer hydraulic conductivity even in cases where relatively few pumping tests and observation locations are available. The use of multiple stimulation frequencies is also shown to improve imaging results.

  8. Characterization of the spontaneous light emission of the PMTs used in the Double Chooz experiment

    NASA Astrophysics Data System (ADS)

    Abe, Y.; Abrahão, T.; Alt, C.; Appel, S.; Bekman, I.; Bergevin, M.; Bezerra, T. J. C.; Bezrukov, L.; Blucher, E.; Brugière, T.; Buck, C.; Busenitz, J.; Cabrera, A.; Calvo, E.; Camilleri, L.; Carr, R.; Cerrada, M.; Chauveau, E.; Chimenti, P.; Collin, A. P.; Conover, E.; Conrad, J. M.; Crespo-Anadón, J. I.; Crum, K.; Cucoanes, A. S.; Damon, E.; Dawson, J. V.; de Kerret, H.; Dhooghe, J.; Dietrich, D.; Djurcic, Z.; dos Anjos, J. C.; Dracos, M.; Etenko, A.; Fallot, M.; Felde, J.; Fernandes, S. M.; Fischer, V.; Franco, D.; Franke, M.; Furuta, H.; Gil-Botella, I.; Giot, L.; Göger-Neff, M.; Gomez, H.; Gonzalez, L. F. G.; Goodenough, L.; Goodman, M. C.; Haag, N.; Hara, T.; Haser, J.; Hellwig, D.; Hofmann, M.; Horton-Smith, G. A.; Hourlier, A.; Ishitsuka, M.; Jiménez, S.; Jochum, J.; Jollet, C.; Kaether, F.; Kalousis, L. N.; Kamyshkov, Y.; Kaneda, M.; Kaplan, D. M.; Kawasaki, T.; Kemp, E.; Kryn, D.; Kuze, M.; Lachenmaier, T.; Lane, C. E.; Lasserre, T.; Letourneau, A.; Lhuillier, D.; Lima, H. P., Jr.; Lindner, M.; López-Castaño, J. M.; LoSecco, J. M.; Lubsandorzhiev, B.; Lucht, S.; Maeda, J.; Mariani, C.; Maricic, J.; Martino, J.; Matsubara, T.; Mention, G.; Meregaglia, A.; Miletic, T.; Minotti, A.; Nagasaka, Y.; Navas-Nicolás, D.; Novella, P.; Nunokawa, H.; Obolensky, M.; Onillon, A.; Osborn, A.; Palomares, C.; Pepe, I. M.; Perasso, S.; Porta, A.; Pronost, G.; Reichenbacher, J.; Reinhold, B.; Röhling, M.; Roncin, R.; Rybolt, B.; Sakamoto, Y.; Santorelli, R.; Schilithz, A. C.; Schönert, S.; Schoppmann, S.; Shaevitz, M. H.; Sharankova, R.; Shrestha, D.; Sibille, V.; Sinev, V.; Skorokhvatov, M.; Smith, E.; Soiron, M.; Spitz, J.; Stahl, A.; Stancu, I.; Stokes, L. F. F.; Strait, M.; Suekane, F.; Sukhotin, S.; Sumiyoshi, T.; Sun, Y.; Svoboda, R.; Terao, K.; Tonazzo, A.; Trinh Thi, H. H.; Valdiviesso, G.; Vassilopoulos, N.; Verdugo, A.; Veyssiere, C.; Vivier, M.; von Feilitzsch, F.; Wagner, S.; Walsh, N.; Watanabe, H.; Wiebusch, C.; Wurm, M.; Yang, G.; Yermia, F.; Zimmer, V.

    2016-08-01

    During the commissioning of the first of the two detectors of the Double Chooz experiment, an unexpected and dominant background caused by the emission of light inside the optical volume has been observed. A specific study of the ensemble of phenomena called Light Noise has been carried out in-situ, and in an external laboratory, in order to characterize the signals and to identify the possible processes underlying the effect. Some mechanisms of instrumental noise originating from the PMTs were identified and it has been found that the leading one arises from the light emission localized on the photomultiplier base and produced by the combined effect of heat and high voltage across the transparent epoxy resin covering the electric components. The correlation of the rate and the amplitude of the signal with the temperature has been observed. For the first detector in operation the induced background has been mitigated using online and offline analysis selections based on timing and light pattern of the signals, while a modification of the photomultiplier assembly has been implemented for the second detector in order to blacken the PMT bases.

  9. Adaptive Augmenting Control Flight Characterization Experiment on an F/A-18

    NASA Technical Reports Server (NTRS)

    VanZwieten, Tannen S.; Gilligan, Eric T.; Wall, John H.; Orr, Jeb S.; Miller, Christopher J.; Hanson, Curtis E.

    2014-01-01

    The NASA Marshall Space Flight Center (MSFC) Flight Mechanics and Analysis Division developed an Adaptive Augmenting Control (AAC) algorithm for launch vehicles that improves robustness and performance by adapting an otherwise welltuned classical control algorithm to unexpected environments or variations in vehicle dynamics. This AAC algorithm is currently part of the baseline design for the SLS Flight Control System (FCS), but prior to this series of research flights it was the only component of the autopilot design that had not been flight tested. The Space Launch System (SLS) flight software prototype, including the adaptive component, was recently tested on a piloted aircraft at Dryden Flight Research Center (DFRC) which has the capability to achieve a high level of dynamic similarity to a launch vehicle. Scenarios for the flight test campaign were designed specifically to evaluate the AAC algorithm to ensure that it is able to achieve the expected performance improvements with no adverse impacts in nominal or nearnominal scenarios. Having completed the recent series of flight characterization experiments on DFRC's F/A-18, the AAC algorithm's capability, robustness, and reproducibility, have been successfully demonstrated. Thus, the entire SLS control architecture has been successfully flight tested in a relevant environment. This has increased NASA's confidence that the autopilot design is ready to fly on the SLS Block I vehicle and will exceed the performance of previous architectures.

  10. Preparation and characterization of a possible topological insulator BiYO3: experiment versus theory.

    PubMed

    Zhang, Y; Deng, S; Pan, M; Lei, M; Kan, X; Ding, Y; Zhao, Y; Köhler, J

    2016-03-01

    The Bi-Y-O system has been investigated by X-ray powder diffraction, electron diffraction, UV-vis and IR experiments. A metastable cubic high temperature phase of BiYO3 with fluorite-type structure has been structurally characterized for the first time and shows a large band gap of ∼5.9 eV. A unified description for the numerous structural variants discovered in the Bi-Y-O system is established within the symmetry breaking approach. This rich structural phenomenon makes the Bi-Y-O system a promising candidate in the search for new topological insulators for applications. On this basis, a long standing controversy on the phase diagram of the Bi-Y-O system has been solved. Our DFT calculations predict a high pressure phase for BiYO3 with perovskite (ABO3) structure and ordering of Bi and Y on the A and B sites, respectively. However, our analysis of the nature of the low energy electronic structure shows that this phase is not a suitable candidate for a topological insulator. PMID:26926091

  11. Experiments on torrefied wood pellet: study by gasification and characterization for waste biomass to energy applications

    PubMed Central

    Rollinson, Andrew N.; Williams, Orla

    2016-01-01

    Samples of torrefied wood pellet produced by low-temperature microwave pyrolysis were tested through a series of experiments relevant to present and near future waste to energy conversion technologies. Operational performance was assessed using a modern small-scale downdraft gasifier. Owing to the pellet's shape and surface hardness, excellent flow characteristics were observed. The torrefied pellet had a high energy density, and although a beneficial property, this highlighted the present inflexibility of downdraft gasifiers in respect of feedstock tolerance due to the inability to contain very high temperatures inside the reactor during operation. Analyses indicated that the torrefaction process had not significantly altered inherent kinetic properties to a great extent; however, both activation energy and pre-exponential factor were slightly higher than virgin biomass from which the pellet was derived. Thermogravimetric analysis-derived reaction kinetics (CO2 gasification), bomb calorimetry, proximate and ultimate analyses, and the Bond Work Index grindability test provided a more comprehensive characterization of the torrefied pellet's suitability as a fuel for gasification and also other combustion applications. It exhibited significant improvements in grindability energy demand and particle size control compared to other non-treated and thermally treated biomass pellets, along with a high calorific value, and excellent resistance to water. PMID:27293776

  12. Flow Characterization of a Detonation Gun Facility and First Coating Experiments

    NASA Astrophysics Data System (ADS)

    Henkes, C.; Olivier, H.

    2014-06-01

    A computer-controlled detonation gun based spraying device has been designed and tested to obtain particle velocities over 1200 m/s. The device is able to be operated in two modes based on different flow-physical principles. In one mode, the device functions like a conventional detonation gun in which the powder is accelerated in a blast wave. In the other mode, an extension of the facility with a nozzle uses the detonated gas for an intermittently operated shock tunnel process in which the particles are injected into and accelerated by a quasi-steady high enthalpy nozzle flow with high reservoir conditions. Presented are experimental results of the operation without nozzle in which the device generates moderate to high particle velocities in an intermittent process with a frequency of 5 Hz. A hydrogen/oxygen mixture and Cu and WC-Co (88/12) powders are used in the experiments. Operation performance and tube outflow are characterized by time-resolved Schlieren images and pressure measurements. The particle velocities in the outflow are obtained by laser Doppler anemometry. Different substrate/powder combinations (Al/Cu, Steel/Cu, Al/WC-Co, and Steel/WC-Co) have been investigated by light microscopy and measurements of microhardness.

  13. Experiments on torrefied wood pellet: study by gasification and characterization for waste biomass to energy applications.

    PubMed

    Rollinson, Andrew N; Williams, Orla

    2016-05-01

    Samples of torrefied wood pellet produced by low-temperature microwave pyrolysis were tested through a series of experiments relevant to present and near future waste to energy conversion technologies. Operational performance was assessed using a modern small-scale downdraft gasifier. Owing to the pellet's shape and surface hardness, excellent flow characteristics were observed. The torrefied pellet had a high energy density, and although a beneficial property, this highlighted the present inflexibility of downdraft gasifiers in respect of feedstock tolerance due to the inability to contain very high temperatures inside the reactor during operation. Analyses indicated that the torrefaction process had not significantly altered inherent kinetic properties to a great extent; however, both activation energy and pre-exponential factor were slightly higher than virgin biomass from which the pellet was derived. Thermogravimetric analysis-derived reaction kinetics (CO2 gasification), bomb calorimetry, proximate and ultimate analyses, and the Bond Work Index grindability test provided a more comprehensive characterization of the torrefied pellet's suitability as a fuel for gasification and also other combustion applications. It exhibited significant improvements in grindability energy demand and particle size control compared to other non-treated and thermally treated biomass pellets, along with a high calorific value, and excellent resistance to water. PMID:27293776

  14. Thermo-osmosis coupled-flow characterization in clay-rocks: experiments and modeling

    NASA Astrophysics Data System (ADS)

    Tremosa, J.; Goncalves, J.; Matray, J.; Violette, S.

    2009-12-01

    Water flow in clay-rocks is not only driven by a hydraulic gradient but also by chemical, thermal or electrical gradients. It implies a re-evaluation of the Darcy law by considering all gradients occurring in the clay-rock and their associated coupling coefficients (e.g. the osmotic efficiency to link a chemical gradient to a water flow). The occurrence of such processes in clay-rocks is due to the low hydraulic conductivity of this media and because of electrical charges at the clay minerals surface. Here, we focused on the thermo-osmosis process, a water flow under a temperature gradient, which is poorly characterized in spite of its implications in nuclear waste storage in clay-rocks. A set of thermo-osmotic experiments was performed in an equipped borehole installed in a Toarcian compacted clay at the IRSN’s Underground Research Laboratory in the south of France. The water flow induced by a temperature gradient (from the hotter towards the colder zone) was reproduced by the help of a numerical model, including coupled-flow processes, mass conservation laws and hydro-thermo-mechanical changes (see Figure). A range of thermo-osmotic permeability (kT), between 6.10-12 and 2.10-10 m2.K-1.s-2, was obtained during the experiments depending on the temperature gradient and uncertainties on the model parameters. Values obtained for the Tournemire’s argillite are in the high range of thermo-osmotic permeabilities for argillaceous materials and suggest an effect of pore size on the thermo-osmotic permeability of a clay-rock (kT being higher with little pore size). Another dependence of thermo-osmotic permeability with temperature is observed, with kT decreasing when the temperature increases. These experiments and modeling indicate thermo-osmosis will have an influence on water flow in presence of a temperature gradient and this process is to consider in water flow studies in clay-rocks. Reference: Tremosa et al. Estimating thermo-osmotic coefficients in clay

  15. Characterization of Psychotic Experiences in Adolescence Using the Specific Psychotic Experiences Questionnaire: Findings From a Study of 5000 16-Year-Old Twins

    PubMed Central

    Ronald, Angelica; Sieradzka, Dominika; Cardno, Alastair G.; Haworth, Claire M. A.; McGuire, Philip; Freeman, Daniel

    2014-01-01

    We aimed to characterize multiple psychotic experiences, each assessed on a spectrum of severity (ie, quantitatively), in a general population sample of adolescents. Over five thousand 16-year-old twins and their parents completed the newly devised Specific Psychotic Experiences Questionnaire (SPEQ); a subsample repeated it approximately 9 months later. SPEQ was investigated in terms of factor structure, intersubscale correlations, frequency of endorsement and reported distress, reliability and validity, associations with traits of anxiety, depression and personality, and sex differences. Principal component analysis revealed a 6-component solution: paranoia, hallucinations, cognitive disorganization, grandiosity, anhedonia, and parent-rated negative symptoms. These components formed the basis of 6 subscales. Correlations between different experiences were low to moderate. All SPEQ subscales, except Grandiosity, correlated significantly with traits of anxiety, depression, and neuroticism. Scales showed good internal consistency, test-retest reliability, and convergent validity. Girls endorsed more paranoia, hallucinations, and cognitive disorganization; boys reported more grandiosity and anhedonia and had more parent-rated negative symptoms. As in adults at high risk for psychosis and with psychotic disorders, psychotic experiences in adolescents are characterized by multiple components. The study of psychotic experiences as distinct dimensional quantitative traits is likely to prove an important strategy for future research, and the SPEQ is a self- and parent-report questionnaire battery that embodies this approach. PMID:24062593

  16. CO2 Field Laboratory at Svelvik Ridge: Site characterization after the first injection experiment

    NASA Astrophysics Data System (ADS)

    Buddensiek, M. L.; Lindeberg, E.; Mørk, A.; Jones, D.; Girard, J. F.; Kuras, O.; Barrio, M.; Royse, K.; Gal, F.; Meldrum, P.; Pezard, P.; Levannier, A.; Desroches, J.; Neyens, D.; Paris, J.; Henry, G.; Bakk, A.; Wertz, F.; Aker, E.; Børresen, M.

    2012-04-01

    The safety and acceptance of CO2 storage will depend on the ability to detect and quantify CO2 within and outside the storage complex. To determine sensitivity of CO2 monitoring systems with respect to CO2 distribution and leakage detection, the CO2 Field Lab project comprises two controlled CO2 injection tests in the shallow (100-300 m) and very shallow (20 m) subsurface of the glacial deposit that forms Svelvik ridge, 50 km south of Oslo. The CO2 displacement in the subsurface and at the surface has and will be monitored with an exhaustive set of techniques. Iteratively, observations and flow modeling will provide frequent updates of the CO2 distribution. The results will be upscaled to assess monitoring systems and requirements with the ultimate objective to provide guidelines to regulators, operators and technology providers for monitoring systems. The formation that comprises the laboratory is a glaciofluvial-glaciomarine terminal deposit formed during the Ski stage of the Holocene deglaciation. Nearby outcrops show that the formation is channeled and variably laminated with a significant variation in grain size and structure. Prior to the injection experiments, the site was characterized including 2D seismic and electric surveys, the drilling, logging and sampling of a 330 m deep appraisal well, core and flow line sample analyses, ground penetrating radar (GPR), a hydrodynamic appraisal, and geochemical and soil gas baseline surveys. These data were used to populate a geomodel. Flow modeling of the plume development included some variability in permeability and anisotropy, and various injection scenarios. Accordingly, the 20 m injection experiment was conducted in fall 2011 with a monitoring plan designed to spatially and temporally monitor the expected plume development. The monitoring equipment was thus distributed around the 20 m deep injection point of an inclined well. It included seven 6 m deep monitoring wells equipped with resistivity, sonic and

  17. Characterizing Design Cognition of High School Students: Initial Analyses Comparing Those with and without Pre-Engineering Experiences

    ERIC Educational Resources Information Center

    Wells, John; Lammi, Matthew; Gero, John; Grubbs, Michael E.; Paretti, Marie; Williams, Christopher

    2016-01-01

    Reported in this article are initial results from of a longitudinal study to characterize the design cognition and cognitive design styles of high school students with and without pre-engineering course experience over a 2-year period, and to compare them with undergraduate engineering students. The research followed a verbal protocol analysis…

  18. Characterizing soil preferential flow using iodine--starch staining experiments and the active region model

    SciTech Connect

    Sheng, Feng; Wang, Kang; Zhang, Renduo; Liu, Hui-Hai

    2009-03-01

    Thirteen iodine-starch staining experiments with different boundary conditions and measurement scales were conducted at two sites to study preferential flow processes in natural unsaturated soils. Digital imaging analyses were implemented to obtain the corresponding preferential flow patterns. The test results are used to evaluate a recently proposed active region model in terms of its usefulness and robustness for characterizing unsaturated flow processes at field scale. Test results provide useful insights into flow patterns in unsaturated soils. They show that flow pattern depends on the top boundary condition. As the total infiltrating-water depth increased form 20 mm to 80 mm for the 100 x 100 cm{sup 2} plots, the corresponding flow pattern changed from few preferential flow paths associated with a relatively small degree of stained coverage and a small infiltration depth, to a pattern characterized by a higher stained coverage and a larger infiltration depth, and to (finally) a relatively homogeneous flow pattern with few unstained area and a much larger infiltration depth. Test results also show that the preferential flow pattern became generally more heterogeneous and complex for a larger measurement scale (or size of infiltration plot). These observations support the general idea behind the active region model that preferential flow pattern in unsaturated soils are dynamic and depend on water flow conditions. Further analyses of the test results indicate that the active-region model is able to capture the major features of the observed flow pattern at the scale of interest, and the determined parameter values do not significantly depend on the test conditions (initial water content and total amount of infiltrating water) for a given test site. This supports the validity of the active region model that considers that parameter to be a property of the corresponding unsaturated soil. Results also show that some intrinsic relation seems to exist between active

  19. Geophysical characterization of soil moisture spatial patterns in a tillage experiment

    NASA Astrophysics Data System (ADS)

    Martinez, G.; Vanderlinden, K.; Giráldez, J. V.; Muriel, J. L.

    2009-04-01

    Knowledge on the spatial soil moisture pattern can improve the characterisation of the hydrological response of either field-plots or small watersheds. Near-surface geophysical methods, such as electromagnetic induction (EMI), provide a means to map such patterns using non-invasive and non-destructive measurements of the soil apparent electrical conductivity (ECa. In this study ECa was measured using an EMI sensor and used to characterize spatially the hydrologic response of a cropped field to an intense shower. The study site is part of a long-term tillage experiment in Southern Spain in which Conventional Tillage (CT), Direct Drilling (DD) and Minimum Tillage (MT) are being evaluated since 1982. Soil ECa was measured before and after a rain event of 115 mm, near the soil surface and at deeper depth (ECas and ECad, respectively) using the EM38-DD EMI sensor. Simultaneously, elevation data were collected at each sampling point to generate a Digital Elevation Model (DEM). Soil moisture during the first survey was close to permanent wilting point and near field capacity during the second survey. For the first survey, both ECas and ECad, were higher in the CT and MT than in the DD plots. After the rain event, rill erosion appeared only in CT and MT plots were soil was uncovered, matching the drainage lines obtained from the DEM. Apparent electrical conductivity increased all over the field plot with higher increments in the DD plots. These plots showed the highest ECas and ECad values, in contrast to the spatial pattern found during the first sampling. Difference maps obtained from the two ECas and ECad samplings showed a clear difference between DD plots and CT and MT plots due to their distinct hydrologic response. Water infiltration was higher in the soil of the DD plots than in the MT and CT plots, as reflected by their ECad increment. Higher ECa increments were observed in the depressions of the terrain, where water and sediments accumulated. On the contrary, the

  20. Adaptive Augmenting Control Flight Characterization Experiment on an F/A-18

    NASA Technical Reports Server (NTRS)

    VanZwieten, Tannen S.; Orr, Jeb S.; Wall, John H.; Gilligan, Eric T.

    2014-01-01

    This paper summarizes the Adaptive Augmenting Control (AAC) flight characterization experiments performed using an F/A-18 (TN 853). AAC was designed and developed specifically for launch vehicles, and is currently part of the baseline autopilot design for NASA's Space Launch System (SLS). The scope covered here includes a brief overview of the algorithm (covered in more detail elsewhere), motivation and benefits of flight testing, top-level SLS flight test objectives, applicability of the F/A-18 as a platform for testing a launch vehicle control design, test cases designed to fully vet the AAC algorithm, flight test results, and conclusions regarding the functionality of AAC. The AAC algorithm developed at Marshall Space Flight Center is a forward loop gain multiplicative adaptive algorithm that modifies the total attitude control system gain in response to sensed model errors or undesirable parasitic mode resonances. The AAC algorithm provides the capability to improve or decrease performance by balancing attitude tracking with the mitigation of parasitic dynamics, such as control-structure interaction or servo-actuator limit cycles. In the case of the latter, if unmodeled or mismodeled parasitic dynamics are present that would otherwise result in a closed-loop instability or near instability, the adaptive controller decreases the total loop gain to reduce the interaction between these dynamics and the controller. This is in contrast to traditional adaptive control logic, which focuses on improving performance by increasing gain. The computationally simple AAC attitude control algorithm has stability properties that are reconcilable in the context of classical frequency-domain criteria (i.e., gain and phase margin). The algorithm assumes that the baseline attitude control design is well-tuned for a nominal trajectory and is designed to adapt only when necessary. Furthermore, the adaptation is attracted to the nominal design and adapts only on an as-needed basis

  1. Temporally and spatially resolved characterization of microwave induced argon plasmas: Experiment and modeling

    NASA Astrophysics Data System (ADS)

    Baeva, M.; Andrasch, M.; Ehlbeck, J.; Loffhagen, D.; Weltmann, K.-D.

    2014-04-01

    Experiments and modeling of the plasma-microwave interaction have been performed in a coaxial microwave plasma source at a field frequency of 2.45 GHz generating argon plasmas at pressures of 20 and 40 millibars and a ratio of flow rate to pressure of 0.125 sccm/Pa. The incident microwave power between 100 W and 300 W is supplied in a regime of a pulse-width modulation with cycle duration of 110 ms and a power-on time of 23 ms. The experiments are based on heterodyne reflectometry and microwave interferometry at 45.75 GHz. They provide the temporal behaviour of the complex reflection coefficient, the microwave power in the plasma, as well as the electron density in the afterglow zone of the discharge. The self-consistent spatially two-dimensional and time-dependent modeling complements the analysis of the plasma-microwave interaction delivering the plasma and electromagnetic field parameters. The consolidating experimental observations and model predictions allow further characterizing the plasma source. The generated plasma has a core occupying the region close to the end of the inner electrode, where maximum electron densities above 1020 m-3 and electron temperatures of about 1 eV are observed. Due to a longer outer electrode of the coaxial structure, the plasma region is extended and fills the volume comprised by the outer electrode. The electron density reaches values of the order of 1019 m-3. The heating of the gas occurs in its great part due to elastic collisions with the plasma electrons. However, the contribution of the convective heating is important especially in the extended plasma region, where the gas temperature reaches its maximum values up to approximately 1400 K. The temporally and spatially resolved modeling enables a thorough investigation of the plasma-microwave interaction which clearly shows that the power in-coupling occurs in the region of the highest electron density during the early stage of the discharge. In the steady state phase

  2. Temporally and spatially resolved characterization of microwave induced argon plasmas: Experiment and modeling

    SciTech Connect

    Baeva, M. Andrasch, M.; Ehlbeck, J.; Loffhagen, D.; Weltmann, K.-D.

    2014-04-14

    Experiments and modeling of the plasma-microwave interaction have been performed in a coaxial microwave plasma source at a field frequency of 2.45 GHz generating argon plasmas at pressures of 20 and 40 millibars and a ratio of flow rate to pressure of 0.125 sccm/Pa. The incident microwave power between 100 W and 300 W is supplied in a regime of a pulse-width modulation with cycle duration of 110 ms and a power-on time of 23 ms. The experiments are based on heterodyne reflectometry and microwave interferometry at 45.75 GHz. They provide the temporal behaviour of the complex reflection coefficient, the microwave power in the plasma, as well as the electron density in the afterglow zone of the discharge. The self-consistent spatially two-dimensional and time-dependent modeling complements the analysis of the plasma-microwave interaction delivering the plasma and electromagnetic field parameters. The consolidating experimental observations and model predictions allow further characterizing the plasma source. The generated plasma has a core occupying the region close to the end of the inner electrode, where maximum electron densities above 10{sup 20} m{sup −3} and electron temperatures of about 1 eV are observed. Due to a longer outer electrode of the coaxial structure, the plasma region is extended and fills the volume comprised by the outer electrode. The electron density reaches values of the order of 10{sup 19} m{sup −3}. The heating of the gas occurs in its great part due to elastic collisions with the plasma electrons. However, the contribution of the convective heating is important especially in the extended plasma region, where the gas temperature reaches its maximum values up to approximately 1400 K. The temporally and spatially resolved modeling enables a thorough investigation of the plasma-microwave interaction which clearly shows that the power in-coupling occurs in the region of the highest electron density during the early stage of

  3. 2D experiments for characterizing solute dispersion in unsaturated heterogeneous porous media

    NASA Astrophysics Data System (ADS)

    Jimenez-Martinez, J.; De Anna, P.; Turuban, R.; Tabuteau, H.; Le Borgne, T.; Meheust, Y.

    2012-12-01

    The unsaturated zone plays a key role in the transfer of chemical elements from the surface to the subsurface. Yet, predicting the transport of chemical species through unsaturated porous media is still an open issue. The distribution of water and air clusters creates flow paths that are controlled by the water saturation, with the feature of a large velocity distribution. As saturation decreases, very low velocity zones in regions of trapped fluid coexist with connected fluid clusters with relatively high velocities. As a consequence the dispersion of solute elements strongly depends on the saturation degree. Numerical simulations of unsaturated flows at the pore scale are feasible, but to our knowledge no simulation of solute transport in the water phase during two-phase flow has been achieved yet. Due to technical difficulties, there also exists relatively few laboratory experiments that allow for visualization and quantification of unsaturated flow and transport at the pore scale. We have developed a two-dimensional (2D) horizontal set up, built by lithographic technique and in which a joint injection of the two phases (wetting and non-wetting) provides a controlled homogeneous saturation in the medium. The simultaneous precise measurement of the flow field, the spatial distribution of water and air, and the 2D tracer concentration field, as well as breakthrough curves at different locations, are used to investigate the relationship between the flow field complexity (velocity distribution and its correlation properties) and dispersion properties. Experimental results show non-Fickian transport behaviors, characterized by heavy tailed breakthrough curves, whose characteristics depend on the average saturation.

  4. Meso-scale hypervelocity cratering experiments (MEMIN project): Characterization of projectile material

    NASA Astrophysics Data System (ADS)

    Domke, Isabelle; Deutsch, Alex; Hecht, Lutz; Kenkmann, Thomas; Berndt, Jasper

    2010-05-01

    The DFG-funded "MEMIN" (multidisciplinary experimental and modelling impact crater research network) research group is aimed at a better understanding of the impact cratering process by combining (i) numerical modelling of crater formation, (ii) investigation of terrestrial craters and (iii) meso-scale hypervelocity impact experiments using the large two-stage light gas gun at the Ernst-Mach-Institute (EMI; Efringen-Kirchen, Germany). In the framework of MEMIN, 1 cm-sized projectiles of the steel SAE 4130 (mass of 4.1 g) have been fired with a velocity of ~ 5.3 km s-1 onto blocks of Seeberger sandstone (size 100 x 100 x 50 cm, grain size 169+/-8 μm; porosity 12-20 vol.%). One goal of MEMIN is to document, analyze, and understand the fate of the projectile and its distribution between crater and ejecta; hence, the use of well-analyzed projectile material is mandatory. For this purpose, we use optical, and electron microscopy, electron microprobe (WWU, and MfN), and LA-ICP-MS microanalysis (WWU). Currently we evaluate which steel or iron meteorite is adequate for the intended use. Important properties of a projectile are (i) textural and chemical homogeneity, (ii) clear chemical distinction to the target sandstone, (iii) presence of elements such as Co, Ni, Cr, PGE that as "meteoritic component" are used in terrestrial craters to trace projectile matter, and characterize the type of the projectile (i.e., meteorite group), and finally (iv) mechanical properties that guarantee stability during sphere production, launch and flight. Strong chemical differences to the target material and geochemical homogeneity of the projectile will allow detection of small volumes of projectile matter by high spatial resolution in-situ analysis with the LA-ICP-MS. Steel SAE 4130 is heterogeneous at the 100-µm scale and has low trace element contents. In future, we plan the use of the alloyed heat treatable steel D290-1 as projectile as its texture is quite homogenous at the scale of

  5. Detailed characterizations of the new Mines Douai comparative reactivity method instrument via laboratory experiments and modeling

    NASA Astrophysics Data System (ADS)

    Michoud, V.; Hansen, R. F.; Locoge, N.; Stevens, P. S.; Dusanter, S.

    2015-08-01

    of corrections (2) and (3). In addition, these simulations reproduce their dependences on the pyrrole-to-OH ratio and on bimolecular reaction rate constants of gases reacting with OH. The good agreement found between laboratory experiments and model simulations gives us confidence in the proposed parameterizations. However, it is worth noting that the numerical values given in this study are suitable for the Mines Douai instrument and may not be appropriate for other CRM instruments. It is recommended that each group characterize its own instrument following the recommendations given in this study. An assessment of performances for the Mines Douai instrument, including a propagation of errors from the different corrections, indicates a limit of detection of 3.0 s-1 and total uncertainties of 17-25 % for OH reactivity values higher than 15 s-1 and NOx mixing ratios lower than 30 ppbv.

  6. Process characterization for metal-affinity chromatography of an Fc fusion protein: a design-of-experiments approach.

    PubMed

    Shukla, A A; Sorge, L; Boldman, J; Waugh, S

    2001-10-01

    The utility of a design-of-experiments approach was investigated for process characterization of a metal-affinity chromatographic purification process for an Fc fusion protein. This approach gave a better understanding of some of the key process variables as well as robustness for this step in the purification process. Single-variable experiments were employed to screen some of the potentially important variables in this step. Ranges for these variables were set based on prior experience in clinical manufacturing with similar processes. Following these experiments, a fractional factorial study was employed to further investigate the most important variables and their interactions. Key operational variables that had an impact on step yield and eluate purity were identified. In addition, the study helped identify a worst-case scenario for the step purity and helped assure that the rest of the process would successfully purify the product. This paper demonstrates the utility of a design-of-experiments approach for the characterization and validation of process chromatography steps in downstream processing. In addition, this study emphasizes the utility of robustness studies early in process development and establishes a strategy for future robustness studies. PMID:11592911

  7. Characterization of the Resource Potential of Martian Soil using the Integrated Dust/Soil Experiment Package (IDEP)

    NASA Technical Reports Server (NTRS)

    Cooper, Bonnie L.; Mckay, David S.; Allen, Carlton C.; Hoffman, John H.; Gittleman, Mark E.

    1997-01-01

    The Integrated Dust/Soil Experiment Package (IDEP) is a suite of instruments that can detect and quantify the abundances of useful raw materials on Mars. We focus here on its capability for resource characterization in the martian soil; however, it is also capable of detecting and quantifying gases in the atmosphere. This paper describes the scientific rationale and the engineering design behind the IDEP.

  8. Hydrogeological characterization of the Heletz Sands Reservoir, Heletz (Israel) as a preliminary step towards CO2 injection experiments

    NASA Astrophysics Data System (ADS)

    Bensabat, Jacob; Niemi, Auli; Tsang, Chin-Fu; Sharma, Prabhakar; Carrera, Jesus; Sauter, Martin; Tatomir, Alexandru; Ghergut, Julia; Pezard, Philippe; Edlman, Katriona; Brauchler, Ralf

    2013-04-01

    Hydrogeological characterization of the Heletz Sands Reservoir, Heletz (Israel) as a preliminary step towards CO2 injection experiments One the major components of the EU-FP7 funded MUSTANG project is to conduct a highly controlled series of CO2 injection experiments, aimed at determining field values of key CO2 trapping mechanisms such as dissolution and residual trapping and to establish a comprehensive and consistent dataset for model validation. Prior to injecting CO2 there is a need to achieve a sufficient degree of hydrogeological characterization of the reservoir. In what follows we present a sequence of hydrologic tests to be conducted at Heletz and their expected contribution to the understanding relevant hydrogeology. These include: 1) Chemical characterization of the formation fluid; 2) Flowing Fluid Electrical Conductivity log, aimed at determining the vertical variability of the reservoir permeability in the near well vicinity; 3) Water pulse and pumping tests, aimed at determining the reservoir scale hydraulic properties; 4) Thermal test, aimed at determining the value of the heat transfer coefficient from the reservoir to the borehole fluid, which is responsible for the heating of injected fluid in the borehole; 5) two-well injection and pumping of water and tracers test, in order to determine the impact of heterogeneity on the hydraulic parameters and to identify preferential flow paths in the reservoir. This paper presents the design and planning of the experiments, the results obtained in field and a preliminary interpretation.

  9. Empirical characterization of oil-shale cratering experiments. [RDX, ANFO, PETN, TNT

    SciTech Connect

    Edwards, C.L.; Craig, J.L.; Lombardo, K.

    1983-01-01

    Numerous small- and intermediate-size cratering experiments have been conducted in Piceance Creek Basin oil shale at the Colony and Anvil Points oil shale mines near Rifle, Colorado. The purpose of these experiments was to evaluate scaling as a tool to infer the behavior of large-scale tests from small-scale experiments, to calibrate the hydrodynamic computer codes used to model explosive fragmentation of oil shale, and to investigate the influence of bedding plane orientation, natural joints, fractures, and the grade of oil shale on rock fragmentation. The small tests were made using PETN and RDX explosive with charge sizes of a few grams. The intermediate-sized tests used ANFO or TNT explosives with charge sizes of 5 to 100 kg. Crater dimensions were measured on all experiments. Crater volumes were calculated from screened rubble volumes on the intermediate-scale experiments and measured directly on the small-scale experiments. Fragment size distributions were measured on most of the intermediate-sized tests and on several of the small-scale experiments. The analyses of these cratering data show: (1) small-scale cratering tests can be used to qualitatively predict the kinds of geologic interactions that will influence a larger-scale experiment; (2) the site specific geology plays a dominant role in the formation of the crater; (3) small flaws and fractures influence crater development and particle size distributions in small-scale craters in the same manner that joint and fracture systems influence intermediate-scale experiments; (4) complex site geology causes increases in the critical and optimum depths of burial and changes the symmetry of the crater; and (5) small- and intermediate-scale cratering experiments can be used to calibrate hydrodynamic computer codes if great care is used to identify the effect of site specific geology. 15 figures, 6 tables.

  10. Thermal Cook-Off Experiments of the HMX Based High Explosive LX-04 to Characterize Violence with Varying Confinement

    NASA Astrophysics Data System (ADS)

    Garcia, Frank; Forbes, Jerry W.

    2005-07-01

    Thermal cook-off experiments were carried out using LX-04 explosive (85% HMX and 15% Viton by weight) with different levels of confinement to characterize the effect of confinement on the reaction violence. These experiments involved heating a porous LX-04 sample in a stainless steel container with varying container end plate thickness and assembly bolt diameter to control overall confinement. As expected, detonation did not occur and reducing the overall confinement lowered the reaction violence. Modeling was also performed using Ignition and Growth kinetics with reasonable agreement to the experiment. These results suggest that controlling the overall system confinement can modify the relative safety in a given scenario. This work was performed under the auspices of the U. S. Department of Energy by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48.

  11. SAMPLING AND ANALYSIS EXPERIMENTS FOR IMPROVED CHARACTERIZATION OF PRODUCTS OF INCOMPLETE COMBUSTION

    EPA Science Inventory

    Laboratory and field experiments were conducted to evaluate the Volatile Organic Sampling Train (VOST) methodology for the sampling and analysis of products of incomplete combustion (PICs). ilot-scale incinerator was used to incinerate several volatile chlorinated organic compoun...

  12. Ion beam plume and efflux characterization flight experiment study. [space shuttle payload

    NASA Technical Reports Server (NTRS)

    Sellen, J. M., Jr.; Zafran, S.; Cole, A.; Rosiak, G.; Komatsu, G. K.

    1977-01-01

    A flight experiment and flight experiment package for a shuttle-borne flight test of an 8-cm mercury ion thruster was designed to obtain charged particle and neutral particle material transport data that cannot be obtained in conventional ground based laboratory testing facilities. By the use of both ground and space testing of ion thrusters, the flight worthiness of these ion thrusters, for other spacecraft applications, may be demonstrated. The flight experiment definition for the ion thruster initially defined a broadly ranging series of flight experiments and flight test sensors. From this larger test series and sensor list, an initial flight test configuration was selected with measurements in charged particle material transport, condensible neutral material transport, thruster internal erosion, ion beam neutralization, and ion thrust beam/space plasma electrical equilibration. These measurement areas may all be examined for a seven day shuttle sortie mission and for available test time in the 50 - 100 hour period.

  13. DebriSat - A Planned Laboratory-Based Satellite Impact Experiment for Breakup Fragment Characterizations

    NASA Technical Reports Server (NTRS)

    Liou, Jer-Chyi; Clark, S.; Fitz-Coy, N.; Huynh, T.; Opiela, J.; Polk, M.; Roebuck, B.; Rushing, R.; Sorge, M.; Werremeyer, M.

    2013-01-01

    The goal of the DebriSat project is to characterize fragments generated by a hypervelocity collision involving a modern satellite in low Earth orbit (LEO). The DebriSat project will update and expand upon the information obtained in the 1992 Satellite Orbital Debris Characterization Impact Test (SOCIT), which characterized the breakup of a 1960 s US Navy Transit satellite. There are three phases to this project: the design and fabrication of DebriSat - an engineering model representing a modern, 60-cm/50-kg class LEO satellite; conduction of a laboratory-based hypervelocity impact to catastrophically break up the satellite; and characterization of the properties of breakup fragments down to 2 mm in size. The data obtained, including fragment size, area-to-mass ratio, density, shape, material composition, optical properties, and radar cross-section distributions, will be used to supplement the DoD s and NASA s satellite breakup models to better describe the breakup outcome of a modern satellite.

  14. Characterization and management of exfoliative cheilitis: a single-center experience.

    PubMed

    Almazrooa, Soulafa A; Woo, Sook-Bin; Mawardi, Hani; Treister, Nathaniel

    2013-12-01

    Exfoliative cheilitis (EC) is a rare inflammatory condition affecting the vermilion of the lips and characterized by production of a thick keratin scale. Given the limited available data, the approach to optimal management of EC remains unclear. The objective of this retrospective study was to characterize the clinical features, management, and outcomes of a series of patients with EC. Fifteen patients with a median age of 59 years and a female-to-male ratio of 2:1 were diagnosed with EC from 2000 to 2010. Parafunctional lip licking (53%) and a history of psychiatric disorders (40%) were common. Ten patients (66%) returned for follow-up, with an overall response rate (partial or complete) of 80% at a median of 2 months, most frequently associated with the use of topical calcineurin inhibitors or moisturizing agents. Management of EC with topical calcineurin inhibitors and moisturizing agents is associated with clinical improvement, but prospective trials are needed. PMID:24237731

  15. Characterization of flow turbulence in large-scale bubble-plume experiments

    NASA Astrophysics Data System (ADS)

    García, Carlos M.; García, Marcelo H.

    2006-07-01

    Flow turbulence generated by a bubble plume in a large tank is characterized. Two different turbulence mechanisms contributing to the mixing and transport process are identified on velocity signals recorded outside of the bubble-plume core: a macro-scale process governed by the wandering motion of the bubble-plume; and an intermediate- and micro-scale process represented by the Kolmogorov power spectrum. A methodology is presented to characterize the different processes and their contributions to the turbulence parameters. The results help to understand the bubble-plume phenomenon and provide a basis to validate numerical models of bubble-plumes used in the design of combined-sewer-overflow reservoirs.

  16. Electrical characterization of GaAs single crystal in direct support of M555 flight experiment

    NASA Technical Reports Server (NTRS)

    Castle, J. G.

    1975-01-01

    The exploration of several nondestructive methods of electrical characterization of semiconductor single crystals was carried out during the period ending May 1974. Two methods of obtaining the microwave skin depth, one for the mapping flat surfaces and the other for analyzing the whole surface of small single crystal wafers, were developed to the stage of working laboratory procedures. The preliminary 35 GHz data characterizing the two types of space-related single crystal surfaces, flat slices of gallium arsenide and small wafers of germanium selenide, are discussed. A third method of nondestructive mapping of donor impurity density in semiconductor surfaces by scanning with a light beam was developed for GaAs; its testing indicates reasonable precision at reasonable scan rates for GaAs surfaces at room temperature.

  17. Electrical characterization of GaAs single crystal in direct support of M555 flight experiment

    NASA Technical Reports Server (NTRS)

    Castle, J. G.

    1975-01-01

    An exploration of several nondestructive methods of electrical characterization of semiconductor single crystals was carried out. Two methods of obtaining the microwave skin depth, one for mapping flat surfaces and the other for analyzing the whole surface of small single crystal wafers, were developed to the stage of working laboratory procedures. The preliminary 35 GHz data characterizing the two types of space related single crystal surfaces, flat slices of gallium arsenide and small wafers of germanium selenide, are discussed. A third method of nondestructive mapping of donor impurity density in semiconductor surfaces by scanning with a light beam was developed for GaAs; its testing indicates reasonable precision at reasonable scan rates for GaAs surfaces at room temperature.

  18. Interstellar silicate analogs for grain-surface reaction experiments: Gas-phase condensation and characterization of the silicate dust grains

    SciTech Connect

    Sabri, T.; Jäger, C.; Gavilan, L.; Lemaire, J. L.; Vidali, G.; Henning, T.

    2014-01-10

    Amorphous, astrophysically relevant silicates were prepared by laser ablation of siliceous targets and subsequent quenching of the evaporated atoms and clusters in a helium/oxygen gas atmosphere. The described gas-phase condensation method can be used to synthesize homogeneous and astrophysically relevant silicates with different compositions ranging from nonstoichiometric magnesium iron silicates to pyroxene- and olivine-type stoichiometry. Analytical tools have been used to characterize the morphology, composition, and spectral properties of the condensates. The nanometer-sized silicate condensates represent a new family of cosmic dust analogs that can generally be used for laboratory studies of cosmic processes related to condensation, processing, and destruction of cosmic dust in different astrophysical environments. The well-characterized silicates comprising amorphous Mg{sub 2}SiO{sub 4} and Fe{sub 2}SiO{sub 4}, as well as the corresponding crystalline silicates forsterite and fayalite, produced by thermal annealing of the amorphous condensates, have been used as real grain surfaces for H{sub 2} formation experiments. A specifically developed ultra-high vacuum apparatus has been used for the investigation of molecule formation experiments. The results of these molecular formation experiments on differently structured Mg{sub 2}SiO{sub 4} and Fe{sub 2}SiO{sub 4} described in this paper will be the topic of the next paper of this series.

  19. Filtration Characterization Method as Tool to Assess Membrane Bioreactor Sludge Filterability—The Delft Experience

    PubMed Central

    Lousada-Ferreira, Maria; Krzeminski, Pawel; Geilvoet, Stefan; Moreau, Adrien; Gil, Jose A.; Evenblij, Herman; van Lier, Jules B.; van der Graaf, Jaap H. J. M.

    2014-01-01

    Prevention and removal of fouling is often the most energy intensive process in Membrane Bioreactors (MBRs), responsible for 40% to 50% of the total specific energy consumed in submerged MBRs. In the past decade, methods were developed to quantify and qualify fouling, aiming to support optimization in MBR operation. Therefore, there is a need for an evaluation of the lessons learned and how to proceed. In this article, five different methods for measuring MBR activated sludge filterability and critical flux are described, commented and evaluated. Both parameters characterize the fouling potential in full-scale MBRs. The article focuses on the Delft Filtration Characterization method (DFCm) as a convenient tool to characterize sludge properties, namely on data processing, accuracy, reproducibility, reliability, and applicability, defining the boundaries of the DFCm. Significant progress was made concerning fouling measurements in particular by using straight forward approaches focusing on the applicability of the obtained results. Nevertheless, a fouling measurement method is still to be defined which is capable of being unequivocal, concerning the fouling parameters definitions; practical and simple, in terms of set-up and operation; broad and useful, in terms of obtained results. A step forward would be the standardization of the aforementioned method to assess the sludge filtration quality. PMID:24957174

  20. Filtration characterization method as tool to assess membrane bioreactor sludge filterability-the delft experience.

    PubMed

    Lousada-Ferreira, Maria; Krzeminski, Pawel; Geilvoet, Stefan; Moreau, Adrien; Gil, Jose A; Evenblij, Herman; van Lier, Jules B; van der Graaf, Jaap H J M

    2014-01-01

    Prevention and removal of fouling is often the most energy intensive process in Membrane Bioreactors (MBRs), responsible for 40% to 50% of the total specific energy consumed in submerged MBRs. In the past decade, methods were developed to quantify and qualify fouling, aiming to support optimization in MBR operation. Therefore, there is a need for an evaluation of the lessons learned and how to proceed. In this article, five different methods for measuring MBR activated sludge filterability and critical flux are described, commented and evaluated. Both parameters characterize the fouling potential in full-scale MBRs. The article focuses on the Delft Filtration Characterization method (DFCm) as a convenient tool to characterize sludge properties, namely on data processing, accuracy, reproducibility, reliability, and applicability, defining the boundaries of the DFCm. Significant progress was made concerning fouling measurements in particular by using straight forward approaches focusing on the applicability of the obtained results. Nevertheless, a fouling measurement method is still to be defined which is capable of being unequivocal, concerning the fouling parameters definitions; practical and simple, in terms of set-up and operation; broad and useful, in terms of obtained results. A step forward would be the standardization of the aforementioned method to assess the sludge filtration quality. PMID:24957174

  1. The Design of Useful Mix Characterization Experiments for the LLNL Reshock Platform

    NASA Astrophysics Data System (ADS)

    Islam, Tanim

    2015-11-01

    The NIF Re-shock platform has been extensively engineered to minimize boundary effects and polluting shocks. It is capable of comprehensively and reproducibly exploring a large parameter space important in mix experiments: strength and timing of shocks and reshocks; the amplitude and wavelength of Richtmyer-Meshkov-unstable interfaces; the Atwood number of these mixing layers; and using a technique developed with experiments at the Omega laser, the simultaneous visualization of spike and bubble fronts. In this work, I explore multimodal and roughened surface designed, and combinations of light and heavy materials, that may illuminate our understanding of mix in plasmas.

  2. Neutron Background Characterization for a Coherent Neutrino-Nucleus Scattering experiment at SNS

    NASA Astrophysics Data System (ADS)

    Gerling, Mark

    2014-03-01

    Coherent Neutrino Nucleus Scattering (CNNS) is a theoretical well-grounded, but as-yet unverified process. The Spallation Neutron Source (SNS) at Oak Ridge National Laboratory (ORNL) may provide an optimal platform for detection of CNNS, possibly with existing detector technology. A proto-collaboration of groups from several institutions has come together to investigate this option and propose an experiment for the first-time observation of CNNS. Currently, the largest risk to such an experiment comes from an unknown background of beam-induced high-energy neutrons that penetrate the existing SNS concrete shielding. We have deployed a neutron scatter camera at the SNS during beam operation and performed preliminary measurements of the neutron backgrounds at a promising experimental location. In order to measure neutrons as high as 100 MeV, we needed to make modifications to the neutron scatter camera and expand its capabilities beyond its standard operating range of 1-14MeV. We have identified sources of high-energy neutrons and continue to investigate other possible locations that may allow a successful CNNS experiment to go forward. The imaging capabilities of the neutron scatter camera will allow more optimal shielding designs that take into account neutron flux anisotropies at the selected experiment locations.

  3. Children's experiences of companion animal maltreatment in households characterized by intimate partner violence.

    PubMed

    McDonald, Shelby Elaine; Collins, Elizabeth A; Nicotera, Nicole; Hageman, Tina O; Ascione, Frank R; Williams, James Herbert; Graham-Bermann, Sandra A

    2015-12-01

    Cruelty toward companion animals is a well-documented, coercive tactic used by abusive partners to intimidate and control their intimate partners. Experiences of co-occurring violence are common for children living in families with intimate partner violence (IPV) and surveys show that more than half are also exposed to abuse of their pets. Given children's relationships with their pets, witnessing such abuse may be traumatic for them. Yet little is known about the prevalence and significance of this issue for children. The present study examines the experiences of children in families with co-occurring pet abuse and IPV. Using qualitative methods, 58 children ages 7-12 who were exposed to IPV were asked to describe their experiences of threats to and harm of their companion animals. Following the interviews, template analysis was employed to systematically develop codes and themes. Coding reliability was assessed using Randolph's free-marginal multirater kappa (kfree=.90). Five themes emerged from the qualitative data, the most common being children's exposure to pet abuse as a power and control tactic against their mother in the context of IPV. Other themes were animal maltreatment to discipline or punish the pet, animal cruelty by a sibling, children intervening to prevent pet abuse, and children intervening to protect the pet during a violent episode. Results indicate that children's experiences of pet abuse are multifaceted, potentially traumatic, and may involve multiple family members with diverse motives. PMID:26520828

  4. From Corrections to Community: The Juvenile Reentry Experience as Characterized by Multiple Systems Involvement

    ERIC Educational Resources Information Center

    Cusick, Gretchen Ruth; Goerge, Robert M.; Bell, Katie Claussen

    2009-01-01

    This Chapin Hall report describes findings on the extent of system involvement among Illinois youth released from correctional facilities, tracking a population of youth under age 18 in Illinois following their release. Using administrative records, researchers develop profiles of reentry experiences across the many systems that serve youth and…

  5. Petrologic and geochemical characterization of the Topopah Spring Member of the Paintbrush Tuff: outcrop samples used in waste package experiments

    SciTech Connect

    Knauss, K.G.

    1984-06-01

    This report summarizes characterization studies conducted with outcrop samples of Topopah Spring Member of the Paintbrush Tuff (Tpt). In support of the Waste Package Task within the Nevada Nuclear Waste Storage Investigation (NNWSI), Tpt is being studied both as a primary object and as a constituent used to condition water that will be reacted with waste form, canister, or packing material. These studies directly or indirectly support NNWSI subtasks concerned with waste package design and geochemical modeling. To interpret the results of subtask experiments, it is necessary to know the exact nature of the starting material in terms of the intial bulk composition, mineralogy, and individual phase geochemistry. 31 figures, 5 tables.

  6. Mössbauer characterization of joints of steel pieces in transient liquid phase bonding experiences

    NASA Astrophysics Data System (ADS)

    di Luozzo, N.; Martínez Stenger, P. F.; Canal, J. P.; Fontana, M. R.; Arcondo, B.

    2011-11-01

    Joining of seamless, low carbon, steel tubes were performed by means of Transient Liquid Phase Bonding process employing a foil of Fe-Si-B metallic glass as filler material. The influence of the main parameters of the process was evaluated: temperature, holding time, pressure and post weld heat treatment. Powder samples were obtained from the joint of tubes and characterized employing Mössbauer Spectroscopy in transmission geometry. The sampling was performed both in tubes successfully welded and in those which show joint defects. The results obtained are correlated with the obtained microstructure and the diffusion of Si and B during the process.

  7. THERMAL COOK-OFF EXPERIMENTS OF THE HMX BASED HIGH EXPLOSIVE LX-04 TO CHARACTERIZE VIOLENCE WITH VARYING CONFINEMENT

    SciTech Connect

    Garcia, F; Vandersall, K S; Forbes, J W; Tarver, C M; Greenwood, D

    2005-07-25

    Thermal cook-off experiments were carried out using LX-04 explosive (85% HMX and 15% Viton by weight) with different levels of confinement to characterize the effect of confinement on the reaction violence. These experiments involved heating a porous LX-04 sample in a stainless steel container with varying container end plate thickness and assembly bolt diameter to control overall confinement. As expected, detonation did not occur and reducing the overall confinement lowered the reaction violence. This is consistent with modeling results that predict that a lower confinement will act to lower the cook-off pressure and thus the overall burn rate which lowers the overall violence. These results suggest that controlling the overall system confinement can modify the relative safety in a given scenario.

  8. Using the MicroASAR on the NASA SIERRA UAS in the Characterization of Arctic Sea Ice Experiment

    NASA Technical Reports Server (NTRS)

    Zaugg, Evan; Long, David; Edwards, Matthew; Fladeland, Matthew; Kolyer, Richard; Crocker, Ian; Maslanik, James; Herzfeld, Ute; Wallin, Bruce

    2010-01-01

    The MicroASAR is a flexible, robust SAR system built on the successful legacy of the BYU microSAR. It is a compact LFM-CW SAR system designed for low-power operation on small, manned aircraft or UAS. The NASA SIERRA UAS was designed to test new instruments and support flight experiments. NASA used the MicroASAR on the SIERRA during a science field campaign in 2009 to study sea ice roughness and break-up in the Arctic and high northern latitudes. This mission is known as CASIE-09 (Characterization of Arctic Sea Ice Experiment 2009). This paper describes the MicroASAR and its role flying on the SIERRA UAS platform as part of CASIE-09.

  9. CHARACTERIZING DETONATING LX-17 CHARGES CROSSING A TRANSVERSE AIR GAP WITH EXPERIMENTS AND MODELING

    SciTech Connect

    Lauderbach, L M; Souers, P C; Garcia, F; Vitello, P; Vandersall, K S

    2009-06-26

    Experiments were performed using detonating LX-17 (92.5% TATB, 7.5% Kel-F by weight) charges with various width transverse air gaps with manganin peizoresistive in-situ gauges present. The experiments, performed with 25 mm diameter by 25 mm long LX-17 pellets with the transverse air gap in between, showed that transverse gaps up to about 3 mm could be present without causing the detonation wave to fail to continue as a detonation. The Tarantula/JWL{sup ++} code was utilized to model the results and compare with the in-situ gauge records with some agreement to the experimental data with additional work needed for a better match to the data. This work will present the experimental details as well as comparison to the model results.

  10. Experiment/Analytical Characterization of the RBCC Rocket-Ejector Mode

    NASA Technical Reports Server (NTRS)

    Ruf, J. H.; Lehman, M.; Pal, S.; Santoro, R. J.; West, J.; Turner, James E. (Technical Monitor)

    2000-01-01

    Experimental and complementary CFD results from the study of the rocket-ejector mode of a Rocket Based Combined Cycle (RBCC) engine are presented and discussed. The experiments involved systematic flowfield measurements in a two-dimensional, variable geometry rocket-ejector system. The rocket-ejector system utilizes a single two-dimensional, gaseous oxygen/gaseous hydrogen rocket as the ejector. To gain a thorough understanding of the rocket-ejector's internal fluid mechanic/combustion phenomena, experiments were conducted with both direct-connect and sea-level static configurations for a range of rocket operating conditions. Overall system performance was obtained through global measurements of wall static pressure profiles, heat flux profiles and engine thrust, whereas detailed mixing and combustion information was obtained through Raman spectroscopy measurements of major species (oxygen, hydrogen, nitrogen and water vapor). The experimental results for both the direct-connect and sea-level static configurations are compared with CFD predictions of the flowfield.

  11. Hydraulic characterization of an activated sludge reactor with recycling system by tracer experiment and analytical models.

    PubMed

    Sánchez, F; Viedma, A; Kaiser, A S

    2016-09-15

    Fluid dynamic behaviour plays an important role in wastewater treatment. An efficient treatment requires the inexistence of certain hydraulic problems such as dead zones or short-circuiting flows. Residence time distribution (RTD) analysis is an excellent technique for detecting these inefficiencies. However, many wastewater treatment installations include water or sludge recycling systems, which prevent us from carrying out a conventional tracer pulse experiment to obtain the RTD curve of the installation. This paper develops an RTD analysis of an activated sludge reactor with recycling system. A tracer experiment in the reactor is carried out. Three analytical models, derived from the conventional pulse model, are proposed to obtain the RTD curve of the reactor. An analysis of the results is made, studying which model is the most suitable for each situation. This paper is useful to analyse the hydraulic efficiency of reactors with recycling systems. PMID:27288672

  12. Analysis of Photothermal Characterization of Layered Materials: Design of Optimal Experiments

    NASA Technical Reports Server (NTRS)

    Cole, Kevin D.

    2003-01-01

    In this paper numerical calculations are presented for the steady-periodic temperature in layered materials and functionally-graded materials to simulate photothermal methods for the measurement of thermal properties. No laboratory experiments were performed. The temperature is found from a new Green s function formulation which is particularly well-suited to machine calculation. The simulation method is verified by comparison with literature data for a layered material. The method is applied to a class of two-component functionally-graded materials and results for temperature and sensitivity coefficients are presented. An optimality criterion, based on the sensitivity coefficients, is used for choosing what experimental conditions will be needed for photothermal measurements to determine the spatial distribution of thermal properties. This method for optimal experiment design is completely general and may be applied to any photothermal technique and to any functionally-graded material.

  13. Preparation and characterization of single crystal samples for high-pressure experiments

    SciTech Connect

    Farber, D; Antonangeli, D; Aracne, C; Benterou, J

    2005-10-26

    To date, most research utilizing the diamond anvil cell (DAC) has been conducted with polycrystalline samples, thus the results are limited to addressing average bulk properties. However, experiments on single crystals can yield data on a range of orientation dependent properties such as thermal and electrical conductivity, magnetic susceptibility, elasticity and plasticity. Here we report new procedures to produce extremely high-quality metallic single crystal samples of size compatible with DAC experiments in the Mbar range. So far, we have produced samples of zinc, Al{sub 2}O{sub 3}, cobalt, molybdenum and cerium, and have evaluated the quality of the finished samples with white-light interferometry, synchrotron x-ray diffraction and inelastic x-ray scattering.

  14. G-Tunnel Welded Tuff Mining Experiment instrumentation evaluations; Yucca Mountain Site Characterization Project

    SciTech Connect

    Zimmerman, R.M.; Bellman, R.A. Jr.; Mann, K.L.; Thompson, T.W.

    1992-04-01

    Designers and analysts of radioactive waste repositories must be able to predict the mechanical behavior of the host rock. Sandia National Laboratory has conducted a mine-by experiment in welded tuff so that information could be obtained regarding the response of the rock to a drill and blast excavation process, where smooth-blasting techniques were used. This report describes the results of the evaluations of nine different instrument or measurement systems used in conjunction with these mining activities.

  15. MISTY ECHO tunnel dynamics experiment data report; Volume 2, Appendices: Yucca Mountain Site Characterization Project

    SciTech Connect

    Phillips, J.S.; Luke, B.A.; Long, J.W.; Lee, J.G.

    1992-04-01

    Tunnel damage resulting from seismic loading is an important issue for the Yucca Mountain nuclear waste repository. The tunnel dynamics experiment was designed to obtain and document ground motions, permanent displacements, observable changes in fracture patterns, and visible damage at ground motion levels of interest to the Yucca Mountain Project. Even though the maximum free-field loading on this tunnel was 28 g, the damage observed was minor. Fielding details, data obtained, and supporting documentation are reported.

  16. MISTY ECHO Tunnel Dynamics Experiment--Data report: Volume 1; Yucca Mountain Site Characterization Project

    SciTech Connect

    Phillips, J.S.; Luke, B.A.; Long, J.W.; Lee, J.G.

    1992-04-01

    Tunnel damage resulting from seismic loading is an important issue for the Yucca Mountain nuclear waste repository. The tunnel dynamics experiment was designed to obtain and document ground motions, permanent displacements, observable changes in fracture patterns, and visible damage at ground motion levels of interest to the Yucca Mountain Project. Even though the maximum free-field loading on this tunnel was 28 g, the damage observed was minor. Fielding details, data obtained, and supporting documentation are reported.

  17. Characterization of an indirect X-ray imaging detector by simulation and experiment.

    PubMed

    Doshi, C; van Riessen, G; Balaur, E; de Jonge, M D; Peele, A G

    2015-01-01

    We describe a comprehensive model of a commercial indirect X-ray imaging detector that accurately predicts the detector point spread function and its dependence on X-ray energy. The model was validated by measurements using monochromatic synchrotron radiation and extended to polychromatic X-ray sources. Our approach can be used to predict the performance of an imaging detector and can be used to optimize imaging experiments with broad-band X-ray sources. PMID:25203971

  18. Ehlers-Danlos syndrome, hypermobility type: A characterization of the patients' lived experience.

    PubMed

    Murray, Brittney; Yashar, Beverly M; Uhlmann, Wendy R; Clauw, Daniel J; Petty, Elizabeth M

    2013-12-01

    Hypermobility type Ehlers-Danlos syndrome (EDS-HT) is an inherited connective tissue disorder clinically diagnosed by the presence of significant joint hypermobility and associated skin manifestations. This article presents a large-scale study that reports the lived experience of EDS-HT patients, the broad range of symptoms that individuals with EDS-HT experience, and the impact these symptoms have on daily functioning. A 237-item online survey, including validated questions regarding pain and depression, was developed. Four hundred sixty-six (466) adults (90% female, 52% college or higher degree) with a self-reported diagnosis of EDS-HT made in a clinic or hospital were included. The most frequently reported symptoms were joint pain (99%), hypermobility (99%), and limb pain (91%). They also reported a high frequency of other conditions including chronic fatigue (82%), anxiety (73%), depression (69%), and fibromyalgia (42%). Forty-six percent of respondents reported constant pain often described as aching and tiring/exhausting. Despite multiple interventions and therapies, many individuals (53%) indicated that their diagnosis negatively affected their ability to work or attend school. Our results show that individuals with EDS-HT can experience a wide array of symptoms and co-morbid conditions. The degree of constant pain and disability experienced by the majority of EDS-HT respondents is striking and illustrates the impact this disorder has on quality of life as well as the clinical challenges inherent in managing this complex connective tissue disorder. PMID:24254846

  19. Characterization of reactive tracers for C-wells field experiments 1: Electrostatic sorption mechanism, lithium

    SciTech Connect

    Fuentes, H.R.; Polzer, W.L.; Essington, E.H.; Newman, B.D.

    1989-11-01

    Lithium (Li{sup +}) was introduced as lithium bromide (LiBr), as a retarded tracer for experiments in the C-wells complex at Yucca Mountain, Nevada Test Site, Nevada. The objective was to evaluate the potential of lithium to sorb predominately by physical forces. lithium was selected as a candidate tracer on the basis of high solubility, good chemical and biological stability, and relatively low sorptivity; lack of bioaccumulation and exclusion as a priority pollutant in pertinent federal environmental regulations; good analytical detectability and low natural background concentrations; and a low cost Laboratory experiments were performed with suspensions of Prow Pass cuttings from drill hole UE-25p{number_sign}1 at depths between 549 and 594 m in J-13 water at a pH of approximately 8 and in the temperature range of 25{degree}C to 45{degree}C. Batch equilibrium and kinetics experiments were performed; estimated thermodynamic constants, relative behavior between adsorption and desorption, and potentiometric studies provided information to infer the physical nature of lithium sorption.

  20. ATTO SECOND ELECTRON BEAMS GENERATION AND CHARACTERIZATION EXPERIMENT AT THE ACCELERATOR TEST FACILITY.

    SciTech Connect

    ZOLOTOREV, M.; ZHOLENTS, A.; WANG, X.J.; BABZIEN, M.; SKARITKA, J.; RAKOWSKY, G.; YAKIMENKO, V.

    2002-02-01

    We are proposing an Atto-second electron beam generation and diagnostics experiment at the Brookhaven Accelerator Test facility (ATF) using 1 {micro}m Inverse Free Electron Laser (IFEL). The proposed experiment will be carried out by an BNL/LBNL collaboration, and it will be installed at the ATF beam line II. The proposed experiment will employ a one-meter long undulator with 1.8 cm period (VISA undulator). The electron beam energy will be 63 MeV with emittance less than 2 mm-mrad and energy spread less than 0.05%. The ATF photocathode injector driving laser will be used for energy modulation by Inverse Free Electron Laser (IFEL). With 10 MW laser peak power, about 2% total energy modulation is expected. The energy modulated electron beam will be further bunched through either a drift space or a three magnet chicane into atto-second electron bunches. The attosecond electron beam bunches will be analyzed using the coherent transition radiation (CTR).

  1. Background Characterization for PROSPECT: a US Short-baseline Neutrino Oscillation Experiment

    NASA Astrophysics Data System (ADS)

    Langford, Thomas

    2014-03-01

    Segmented antineutrino detectors placed near compact research reactors provide an excellent opportunity to probe short-baseline neutrino oscillations and precisely measure the reactor antineutrino spectrum. The PROSPECT collaboration has developed a conceptual design for an experiment covering the favored reactor anomaly parameter space using two detectors located within 4-20 m of an existing reactor. Research reactors offer the benefits of compact cores, distinct reactor-off periods, and single-isotope fuel. However, they are typically located at ground level, providing little to no overburden to shield detectors. This talk will present the background requirements of the PROSPECT experiment and discuss feasibility studies that have been performed for three potential locations: NIST, INL, and ORNL. Two fast neutron detectors, a muon telescope, and HPGE and NaI gamma detectors have been deployed at the sites to measure reactor-related and cosmogenic backgrounds. The results of background measurements at each site during reactor operation and shutdown will be shown. Additionally, the planned techniques to reduce the impact of each background on the physics reach of the full experiment will also be discussed.

  2. Day and night characterization of smoke aerosols during the BORTAS experiment

    NASA Astrophysics Data System (ADS)

    Saha, Auromeet; Baibakov, Konstantin; Blanchard, Yann; Ivanescu, Liviu; O'Neill, Norman T.; Daou, David; Duck, Thomas J.; Strawbridge, Kevin S.

    2013-04-01

    During the summer of 2011 the BORTAS (BOReal forest fires on Tropospheric oxidants over the Atlantic using Aircraft and Satellites) field campaign was carried out over eastern Canada and the western Atlantic. Both passive and active optical measurements were carried out at Halifax, NS using ground-based sun/star photometry and backscatter (Raman) lidar. We employed these ground-based measurements along with supporting information such as CALIPSO (satellite based) lidar profiles, MODIS imagery, chemical transport modeling and backtrajectories to characterize the day / night transition of smoke plumes over Halifax during an extended series of smoke events that lasted from July 17 to 26 (traceable to forest fires in western Ontario). Preliminary analysis indicates a physical coherence between these diverse data elements including day / night continuity of the submicron (smoke) optical depths derived from the sun and star photometer spectra and covariance between the passively derived optical depths and those from the Raman lidar.

  3. Location Capability and Site Characterization Installing a Borehole VBB Seismometer: the OGS Experience in Ferrara (Italy)

    NASA Astrophysics Data System (ADS)

    Pesaresi, D.; Barnaba, C.

    2014-12-01

    The Centro di Ricerche Sismologiche (CRS, Seismological Research Centre) of the Istituto Nazionale di Oceanografia e di Geofisica Sperimentale (OGS, Italian National Institute for Oceanography and Experimental Geophysics) in Udine (Italy) after the strong earthquake of magnitude M=6.4 occurred in 1976 in the Italian Friuli-Venezia Giulia region, started to operate the Northeastern Italy Seismic Network: it currently consists of 19 very sensitive broad band and 17 simpler short period seismic stations, all telemetered to and acquired in real time at the OGS CRS data centre in Udine. The southwestern edge of the OGS seismic network stands on the Po alluvial basin: earthquake localization and characterization in this area is affected by the presence of soft alluvial deposits. Following the ML=5.9 earthquake that struck the Emilia region around Ferrara in Northern Italy on May 20, 2012, a cooperation of Istituto Nazionale di Geofisica e Vulcanologia, OGS, the Comune di Ferrara and the University of Ferrara lead to the reinstallation of a previously existing very broad band (VBB) borehole seismic station in Ferrara and to the deployment of a temporary seismographic network consisting of eight portable seismological stations, to record the local earthquakes that occurred during the seismic sequence. The aim of the OGS intervention was on one hand to extend its real time seismic monitoring capabilities toward South-West, including Ferrara and its surroundings, and on the other hand to evaluate seismic site responses in the area. We will introduce details of the Ferrara VBB borehole station and the OGS temporary seismographic network configuration and installation. We will then illustrate the location capability performances, and finally we will shortly describe seismic site characterization with surface/borehole comparisons in terms of seismic noise, site amplification and resonance frequencies.

  4. Design and characterization of a W-band system for modulated DNP experiments

    NASA Astrophysics Data System (ADS)

    Guy, Mallory L.; Zhu, Lihuang; Ramanathan, Chandrasekhar

    2015-12-01

    Magnetic-field and microwave-frequency modulated DNP experiments have been shown to yield improved enhancements over conventional DNP techniques, and even to shorten polarization build-up times. The resulting increase in signal-to-noise ratios can lead to significantly shorter acquisition times in signal-limited multi-dimensional NMR experiments and pave the way to the study of even smaller sample volumes. In this paper we describe the design and performance of a broadband system for microwave frequency- and amplitude-modulated DNP that has been engineered to minimize both microwave and thermal losses during operation at liquid helium temperatures. The system incorporates a flexible source that can generate arbitrary waveforms at 94 GHz with a bandwidth greater than 1 GHz, as well as a probe that efficiently transmits the millimeter waves from room temperature outside the magnet to a cryogenic environment inside the magnet. Using a thin-walled brass tube as an overmoded waveguide to transmit a hybrid HE11 mode, it is possible to limit the losses to 1 dB across a 2 GHz bandwidth. The loss is dominated by the presence of a quartz window used to isolate the waveguide pipe. This performance is comparable to systems with corrugated waveguide or quasi-optical components. The overall excitation bandwidth of the probe is seen to be primarily determined by the final antenna or resonator used to excite the sample and its coupling to the NMR RF coil. Understanding the instrumental limitations imposed on any modulation scheme is key to understanding the observed DNP results and potentially identifying the underlying mechanisms. We demonstrate the utility of our design with a set of triangular frequency-modulated DNP experiments.

  5. Design and characterization of a W-band system for modulated DNP experiments.

    PubMed

    Guy, Mallory L; Zhu, Lihuang; Ramanathan, Chandrasekhar

    2015-12-01

    Magnetic-field and microwave-frequency modulated DNP experiments have been shown to yield improved enhancements over conventional DNP techniques, and even to shorten polarization build-up times. The resulting increase in signal-to-noise ratios can lead to significantly shorter acquisition times in signal-limited multi-dimensional NMR experiments and pave the way to the study of even smaller sample volumes. In this paper we describe the design and performance of a broadband system for microwave frequency- and amplitude-modulated DNP that has been engineered to minimize both microwave and thermal losses during operation at liquid helium temperatures. The system incorporates a flexible source that can generate arbitrary waveforms at 94GHz with a bandwidth greater than 1GHz, as well as a probe that efficiently transmits the millimeter waves from room temperature outside the magnet to a cryogenic environment inside the magnet. Using a thin-walled brass tube as an overmoded waveguide to transmit a hybrid HE11 mode, it is possible to limit the losses to 1dB across a 2GHz bandwidth. The loss is dominated by the presence of a quartz window used to isolate the waveguide pipe. This performance is comparable to systems with corrugated waveguide or quasi-optical components. The overall excitation bandwidth of the probe is seen to be primarily determined by the final antenna or resonator used to excite the sample and its coupling to the NMR RF coil. Understanding the instrumental limitations imposed on any modulation scheme is key to understanding the observed DNP results and potentially identifying the underlying mechanisms. We demonstrate the utility of our design with a set of triangular frequency-modulated DNP experiments. PMID:26524649

  6. Hydrocarbon characterization experiments in fully turbulent fires : results and data analysis.

    SciTech Connect

    Suo-Anttila, Jill Marie; Blanchat, Thomas K.

    2011-03-01

    As the capabilities of numerical simulations increase, decision makers are increasingly relying upon simulations rather than experiments to assess risks across a wide variety of accident scenarios including fires. There are still, however, many aspects of fires that are either not well understood or are difficult to treat from first principles due to the computational expense. For a simulation to be truly predictive and to provide decision makers with information which can be reliably used for risk assessment the remaining physical processes must be studied and suitable models developed for the effects of the physics. The model for the fuel evaporation rate in a liquid fuel pool fire is significant because in well-ventilated fires the evaporation rate largely controls the total heat release rate from the fire. This report describes a set of fuel regression rates experiments to provide data for the development and validation of models. The experiments were performed with fires in the fully turbulent scale range (> 1 m diameter) and with a number of hydrocarbon fuels ranging from lightly sooting to heavily sooting. The importance of spectral absorption in the liquid fuels and the vapor dome above the pool was investigated and the total heat flux to the pool surface was measured. The importance of convection within the liquid fuel was assessed by restricting large scale liquid motion in some tests. These data sets provide a sound, experimentally proven basis for assessing how much of the liquid fuel needs to be modeled to enable a predictive simulation of a fuel fire given the couplings between evaporation of fuel from the pool and the heat release from the fire which drives the evaporation.

  7. Conversion-electron experiment to characterize the decay of the /sup 237/Np shape isomer

    SciTech Connect

    Henry, E.A.; Becker, J.A.; Bauer, R.W.; Gardner, D.G.; Decman, D.J.; Meyer, R.A.; Roy, N.; Sale, K.E.

    1987-12-08

    Conversion electrons from the decay of low-lying levels of /sup 237/Np have been measured to detect the population of these levels by gamma-ray decay of the /sup 237/Np shape isomer. Analysis of the 208-keV transition L conversion-electron peak gives an upper limit of about 17 ..mu..b for the population of the 3/2/sup -/ 267-keV level in /sup 237/Np from the shape isomer decay. Model calculations are compared with the measured limit. Improvements are suggested for this experiment. 9 refs., 4 figs.

  8. Characterization of good teleoperators - What aptitudes, interests, and experience correlate with measures of teleoperator performance

    NASA Technical Reports Server (NTRS)

    Yorchak, J. P.; Hartley, C. S.; Hinman, E.

    1985-01-01

    The use of aptitude tests and questionnaries to evaluate an individuals aptitude for teleoperation is studied. The Raven Progressive Matrices Test and Differential Aptitude Tests, and a 16-item questionnaire for assessing the subject's interests, academic background, and previous experience are described. The Proto-Flight Manipulator Arm, cameras, console, hand controller, and task board utilized by the 17 engineers are examined. The correlation between aptitude scores and questionnaire responses, and operator performance is investigated. Multiple regression data reveal that the eight predictor variables are not individually significant for evaluating operator performance; however, the complete test battery is applicable for predicting 49 percent of subject variance on the criterion task.

  9. Characterization of a scintillating fibers read by MPPC detectors trigger prototype for the AMADEUS experiment

    NASA Astrophysics Data System (ADS)

    Bazzi, M.; Berucci, C.; Curceanu, C.; D'Uffizi, A.; Piscicchia, K.; Poli Lener, M.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Vazquez Doce, O.

    2013-05-01

    Multi-Pixel Photon Counters (MPPC) consist of hundreds of micro silicon Avalanche PhotoDiodes (APD) working in Geiger mode. The high gain and the low noise, typical of these devices, together with their good performance in magnetic field, make them ideal readout detectors for scintillating fibers as trigger detectors in particle and nuclear physics experiments like AMADEUS, where such detectors are planned to be used to trigger on charged kaon pairs. In order to investigate the detection efficiency of such a system, a prototype setup consisting of 32, 1 mm diameter scintillating fibers, arranged in two double layers of 16 fibers each, and read out at both sides by 64 MPPCs with an ad-hoc built readout electronics, was tested at the πM-1 line of the Paul Scherrer Institute (PSI) in Villigen, Switzerland. The detection efficiency and the trigger capability were measured on a beam containing protons, electrons, muons and pions with a momentum of 440 MeV/c. The measured average efficiency for protons for a double layer of scintillating fibers (96.2±1.0%) represents a guarantee of the good performance of this system as a trigger for the AMADEUS experiment.

  10. Characterization of mineral dust aerosols during the Saharan Dust Experiment (SHADE)

    NASA Astrophysics Data System (ADS)

    Léon, J.-F.; Tanré, D.; Haywood, J.; Pelon, J.; Kaufman, Y. J.

    2003-04-01

    Aerosols are known to be important in determining the Earth’s radiative balance. Dust aerosols are particularly interesting since, in addition to their scattering and absorbing properties that affect the solar radiation, they also perturb the terrestrial radiation. In addition, recent studies have shown that a significant proportion of mineral dust in the atmosphere may be of anthropogenic origin and therefore they may have an important role in climate change by exerting a significant radiative forcing. The Saharan Dust Experiment was designed to better determine the parameters that are relevant for computing the direct radiative effect of mineral dust. Two aircraft combining in situ measurements and remote sensing instruments were coordinated with satellite overpasses during the experiment which was based in Cape Verde during the period September 20-28, 2000. These in-situ and remotely sensed data provide valuable information on the microphysical, optical properties and radiative effects of a very large mineral dust outbreak with aerosol optical thickness up to 1.5. A new approach based on a synergy between active (lidar) and passive (spaceborne radiometer) remote sensing has been used to investigate the vertical structure of the dust plume. The retrieved profiles of extinction compare well with in situ aircraft measurements. Profiles derived from lidar measurements on September 25 highlight the presence of the so-called Saharan Air Layer, located between 2.2 and 4.5 km. Another dust layer within the sub-Saharan transition layer over the marine boundary layer is also observed. In this second layer, the effective radius of particles is significantly smaller than in the aloft layer. The trajectory analyses and the Total Mapping Ozone Spectrometer Aerosol Index suggest that the aerosols present at 1500m originates from West Mauritania. The higher aerosol layer originates from southern Algeria which confirms the difference of altitude of the dust transport

  11. Characterization of the general purpose research furnace for low-G directional solidification experiments

    NASA Technical Reports Server (NTRS)

    Crouch, R. K.; Fripp, A. L.; Debnam, W. J.; Clark, I. O.; Zweiner, J. W.; Carlson, F. M.

    1983-01-01

    Preliminary tests have shown that it is possible to control the temperature settings in NASA's General Purpose Research Furnace in such a manner as to obtain a constant rate of movement of a high temperature isotherm down the length of the furnace. These tests also showed that a temperature gradient on the order of 40 deg cm could be obtained in the furnace while moving the particular isotherm of interest, i.e., 900 C. This provides the possibility of performing directional solidification experiments in the furnace on a MEA flight in the low gravity environment provided by the Shuttle. A functional dependency of growth rate on perturbations during the the growth has been derived and shows the importance of avoiding non-steady growth rates and changing temperature gradients.

  12. Characterization and parametric dependencies of low wavenumber pedestal turbulence in the National Spherical Torus Experiment

    SciTech Connect

    Smith, D. R.; Fonck, R. J.; McKee, G. R.; Thompson, D. S.; Bell, R. E.; Diallo, A.; Guttenfelder, W.; Kaye, S. M.; LeBlanc, B. P.; Podesta, M.

    2013-05-15

    The spherical torus edge region is among the most challenging regimes for plasma turbulence simulations. Here, we measure the spatial and temporal properties of ion-scale turbulence in the steep gradient region of H-mode pedestals during edge localized mode-free, MHD quiescent periods in the National Spherical Torus Experiment. Poloidal correlation lengths are about 10 ρ{sub i}, and decorrelation times are about 5 a/c{sub s}. Next, we introduce a model aggregation technique to identify parametric dependencies among turbulence quantities and transport-relevant plasma parameters. The parametric dependencies show the most agreement with transport driven by trapped-electron mode, kinetic ballooning mode, and microtearing mode turbulence, and the least agreement with ion temperature gradient turbulence. In addition, the parametric dependencies are consistent with turbulence regulation by flow shear and the empirical relationship between wider pedestals and larger turbulent structures.

  13. Site Characterization of the Source Physics Experiment Phase II Location Using Seismic Reflection Data

    SciTech Connect

    Sexton, Emily; Snelson, Catherine M; Chipman, Veraun D; Emer, Dudley; White, Bob; Emmit, Ryan; Wright, Al; Drellack, Sigmund; Huckins-Gang, Heather; Mercadante, Jennifer; Floyd, Michael; McGowin, Chris; Cothrun, Chris; Bonal, Nedra

    2013-12-05

    An objective of the Source Physics Experiment (SPE) is to identify low-yield nuclear explosions from a regional distance. Low-yield nuclear explosions can often be difficult to discriminate among the clutter of natural and man-made explosive events (e.g., earthquakes and mine blasts). The SPE is broken into three phases. Phase I has provided the first of the physics-based data to test the empirical models that have been used to discriminate nuclear events. The Phase I series of tests were placed within a highly fractured granite body. The evolution of the project has led to development of Phase II, to be placed within the opposite end member of geology, an alluvium environment, thereby increasing the database of waveforms to build upon in the discrimination models. Both the granite and alluvium sites have hosted nearby nuclear tests, which provide comparisons for the chemical test data. Phase III of the SPE is yet to be determined.

  14. Novel Experiments to Characterize Creep-Fatigue Degradation in VHTR Alloys

    SciTech Connect

    J. K. Wright; J. A. Simpson; L. J. Carroll; R. N. Wright; T.-L. Sham

    2013-10-01

    It is well known in energy systems that the creep lifetime of high temperature alloys is significantly degraded when a cyclic load is superimposed on components operating in the creep regime. A test method has been developed in an attempt to characterize creep-fatigue behavior of alloys at high temperature. The test imposes a hold time during the tensile phase of a fully reversed strain-controlled low cycle fatigue test. Stress relaxation occurs during the strain-controlled hold period. This type of fatigue stress relaxation test tends to emphasize the fatigue portion of the total damage and does not necessarily represent the behavior of a component in-service well. Several different approaches to laboratory testing of creep-fatigue at 950°C have been investigated for Alloy 617, the primary candidate for application in VHTR heat exchangers. The potential for mode switching in a cyclic test from strain control to load control, to allow specimen extension by creep, has been investigated to further emphasize the creep damage. In addition, tests with a lower strain rate during loading have been conducted to examine the influence of creep damage occurring during loading. Very short constant strain hold time tests have also been conducted to examine the influence of the rapid stress relaxation that occurs at the beginning of strain holds.

  15. Morphological characterization of soot aerosol particles during LACIS Experiment in November (LExNo)

    NASA Astrophysics Data System (ADS)

    Kiselev, A.; Wennrich, C.; Stratmann, F.; Wex, H.; Henning, S.; Mentel, T. F.; Kiendler-Scharr, A.; Schneider, J.; Walter, S.; Lieberwirth, I.

    2010-06-01

    Combined mobility and aerodynamic measurements were used to characterize the morphology of soot particles in an experimental campaign on the hygroscopic growth and activation of an artificial biomass burning aerosol. A custom-made, single-stage low-pressure impactor and two aerosol mass spectrometers (AMS) operating in the free molecular regime were used to measure the vacuum aerodynamic diameter of mobility-selected artificial soot particles that were produced in a spark discharge generator and then modified by condensation of ammonium hydrogen sulfate or levoglucosan as a coating to change their hydroscopic activity. Transmission electron microscope images revealed a relationship between the electrical mobility diameter and the diameter of the enveloping sphere, thus enabling evaluation of the effective density of soot agglomerates. A fractal description of the morphology of the soot aggregates allowed for evaluation of the average mass of the hygroscopic material per particle. The average mass of the hygroscopic material per particle was also measured directly with the two AMS instruments, and the agreement between the two methods was found satisfactory. This tandem approach allows detection of small changes in the particle effective density and morphology caused by condensation of organic material.

  16. A new inverse approach for the localization and characterization of defects based on compressive experiments

    NASA Astrophysics Data System (ADS)

    Barbarella, E.; Allix, O.; Daghia, F.; Lamon, J.; Jollivet, T.

    2016-06-01

    Compressive tests involving buckling are known to be defect sensitive, nevertheless, to our knowledge, no inverse approach has been proposed yet to use this property for the localization and characterization of material defects. This is due to geometric imperfections, which greatly influence and even dominate the response of defective parts under compression. In comparison with a system lacking geometric imperfections, the modified system does not present any bifurcation, showing that the non-linear progressive response is mainly governed by such imperfections. Before implementing any inverse procedures it is necessary to know whether extracting meaningful material defect information from compressive tests on specimen which also have geometric imperfections is possible. To tackle this issue, an equivalent eigenvalue problem will be extracted from the non-linear response, a problem corrected from geometric imperfections. A dedicated inverse formulation based on the modified constitutive relation error will then be constructed which will involve only well-posed linear problems. Examples illustrate the potential of the methodology to localize and identify single and multiple defects.

  17. On the quantitative characterization of human body sway in experiments with long-term performance.

    PubMed

    Seidel, H; Bräuer, D; Bastek, R; Issel, I

    1978-01-01

    Two different conditions of standing were examined in young male subjects with an intact vestibular system by means of stabilography. Low-frequency sways were eliminated by means of a digital high-pass filter. The parameters of the histograms of amplitudes, the autocorrelation functions, and the power-spectral density were estimated. The quantitative parameters of the stabilograms are compared for the two standing positions, both for the frontal and sagittal planes. Quotients of estimates of variance components calculated on the basis of analysis of variance are stated as measures of reliability for the parameters of histograms of amplitudes and power-spectral density in defined frequency bands. The reliability of the standard deviations of amplitudes was much better than the reliability of skewness and excess. The reliability of spectral density differed for frequency ranges investigated. Generally, the quotients of estimates of variance components were higher for the spectral density in the frequency bands of sagittal stabilograms than in those of frontal ones. Performance significantly affected several parameters of histograms and the distributions of power-spectral density. Standard deviation of amplitudes and power-spectral density proved to be suitable for the quantitative characterization of stabilograms. PMID:752208

  18. Nocturnal Boundary Layer Measurements during the Amazonian Aerosol Characterization Experiment (AMAZE)

    NASA Astrophysics Data System (ADS)

    Tota, J.; Fisch, G.; Santos, R.; Silva Dias, M.

    2009-05-01

    To characterize the Nocturnal Boundary Layer (NBL) hourly profiles of wind, pressure, temperature, humidity and 5 sizes particles concentration, were made by using tethered balloon at INPA tropical Amazon rainforest Reserve (Cuieiras) 100 km northwest from Manaus city. The measurements were made during the wet season March/2008. The NBL height was 100 to 150m, with a very well mixed layer close to surface associate with temperature inversion. The wind profiles shows a very clear low level in two nights, about 500 to 900 m, and, in general, all nights show an stable and cooler air layer close the surface uncoupled with outer residual boundary layer above. At the site a very clear drainage flow from north quadrant down slope eastward quadrant during very the stable cases. This findings is correlates with particles profiles where was commonly trapped by stable layer presenting high concentrations, for all 5 sizes measured, close to the surface at vegetation level and just above it. All nights presents high humidity with fog formation in three cases, associates with temperature below the 23C. The wind speed were very low about 0.5 to calm, in generally associate with drainage flow down hill. The NBL dynamics is a discussion issue associate to the aerosol nocturnal mixing in complex terrain with tall vegetation, the currently AMAZE site case.

  19. Nocturnal Boundary Layer Measurements during the Amazonian Aerosol Characterization Experiment (amaze)

    NASA Astrophysics Data System (ADS)

    Tota, J.; Santos, R.; Fisch, G.; Querino, C.; Silva Dias, M.; Artaxo, P.; Guenther, A.; Martin, S.; Manzi, A.

    2008-12-01

    To characterize the Nocturnal Boundary Layer (NBL) hourly profiles of wind, pressure, temperature, humidity and 5 sizes particles concentration, were made by using tethered balloon at INPA tropical Amazon rainforest Reserve (Cuieiras) 100 km northwest from Manaus city. The measurements were made during the wet season March/2008. The NBL height was 100 to 150m, with a very well mixed layer close to surface associate with temperature inversion. The wind profiles shows a very clear low level in two nights, about 500 to 900 m, and, in general, all nights show an stable and cooler air layer close the surface uncoupled with outer residual boundary layer above. At the site a very clear drainage flow from north quadrant down slope eastward quadrant during very the stable cases. This findings is correlates with particles profiles where was commonly trapped by stable layer presenting high concentrations, for all 5 sizes measured, close to the surface at vegetation level and just above it. All nights presents high humidity with fog formation in three cases, associates with temperature below the 23°C. The wind speed were very low about 0.5 to calm, in generally associate with drainage flow down hill. The NBL dynamics is a discussion issue associate to the aerosol nocturnal mixing in complex terrain with tall vegetation, the currently AMAZE site case.

  20. VOC Source and Inflow Characterization during the Deep Convective Cloud and Chemistry (DC3) experiment

    NASA Astrophysics Data System (ADS)

    Blake, N. J.; Hartt, G.; Barletta, B.; Simpson, I. J.; Schroeder, J.; Hung, Y.; Marrero, J.; Gartner, A.; Hirsch, C.; Meinardi, S.; Blake, D. R.; Zhang, Y.; Apel, E. C.; Hornbrook, R. S.; Campos, T. L.; Emmons, L. K.

    2013-12-01

    More than 50 volatile organic compounds (VOCs) were measured during the Deep Convective Clouds and Chemistry Project (DC3) field campaign, which was based out of Salina, KS May 10 - June 30, 2012. DC3 investigated the impact of deep, mid-latitude continental convective clouds on upper tropospheric composition and chemistry. The UCI Whole Air Sampler (WAS) measured VOCs on board the NASA DC-8 aircraft and the NCAR Trace Organic Gas Analyzer (TOGA) measured VOCs on board the NSF GV. Coordinated flights between the two aircraft produced a rich dataset with which to characterize the inflow and outflow of convective events. While probing storm inflow, numerous natural and anthropogenic sources were encountered, including oil and gas wells in Colorado, Texas, and Oklahoma, biomass burning, biogenic VOC emissions, and other anthropogenic sources (urban, feedlots, etc). The significant and widespread influence of oil and gas activities dominated VOC alkane distributions during DC3, in both inflow and outflow, effectively illustrating the connection between emission and fast vertical transport of VOCs into the free troposphere. We present a mass balance analysis of a flight over TX and OK, which allowed us to estimate oil and gas emissions in that region. The results from this analysis will be compared to previous work in the same area, as well as to emissions from other oil and gas regions and to model simulations from the Community Atmosphere Model with Chemistry (CAM-chem).

  1. Characterizing the Conformational Landscape of Flavivirus Fusion Peptides via Simulation and Experiment

    NASA Astrophysics Data System (ADS)

    Marzinek, Jan K.; Lakshminarayanan, Rajamani; Goh, Eunice; Huber, Roland G.; Panzade, Sadhana; Verma, Chandra; Bond, Peter J.

    2016-01-01

    Conformational changes in the envelope proteins of flaviviruses help to expose the highly conserved fusion peptide (FP), a region which is critical to membrane fusion and host cell infection, and which represents a significant target for antiviral drugs and antibodies. In principle, extended timescale atomic-resolution simulations may be used to characterize the dynamics of such peptides. However, the resultant accuracy is critically dependent upon both the underlying force field and sufficient conformational sampling. In the present study, we report a comprehensive comparison of three simulation methods and four force fields comprising a total of more than 40 μs of sampling. Additionally, we describe the conformational landscape of the FP fold across all flavivirus family members. All investigated methods sampled conformations close to available X-ray structures, but exhibited differently populated ensembles. The best force field / sampling combination was sufficiently accurate to predict that the solvated peptide fold is less ordered than in the crystallographic state, which was subsequently confirmed via circular dichroism and spectrofluorometric measurements. Finally, the conformational landscape of a mutant incapable of membrane fusion was significantly shallower than wild-type variants, suggesting that dynamics should be considered when therapeutically targeting FP epitopes.

  2. Characterizing the Conformational Landscape of Flavivirus Fusion Peptides via Simulation and Experiment

    PubMed Central

    Marzinek, Jan K.; Lakshminarayanan, Rajamani; Goh, Eunice; Huber, Roland G.; Panzade, Sadhana; Verma, Chandra; Bond, Peter J.

    2016-01-01

    Conformational changes in the envelope proteins of flaviviruses help to expose the highly conserved fusion peptide (FP), a region which is critical to membrane fusion and host cell infection, and which represents a significant target for antiviral drugs and antibodies. In principle, extended timescale atomic-resolution simulations may be used to characterize the dynamics of such peptides. However, the resultant accuracy is critically dependent upon both the underlying force field and sufficient conformational sampling. In the present study, we report a comprehensive comparison of three simulation methods and four force fields comprising a total of more than 40 μs of sampling. Additionally, we describe the conformational landscape of the FP fold across all flavivirus family members. All investigated methods sampled conformations close to available X-ray structures, but exhibited differently populated ensembles. The best force field / sampling combination was sufficiently accurate to predict that the solvated peptide fold is less ordered than in the crystallographic state, which was subsequently confirmed via circular dichroism and spectrofluorometric measurements. Finally, the conformational landscape of a mutant incapable of membrane fusion was significantly shallower than wild-type variants, suggesting that dynamics should be considered when therapeutically targeting FP epitopes. PMID:26785994

  3. Characterization of Shock Effects in Calcite by Raman Spectroscopy: Results of Experiments

    NASA Technical Reports Server (NTRS)

    Bell, M. S.

    2016-01-01

    Carbonates comprise approx. 20% by volume of present day Earth's sedimentary rocks and store most of the terrestrial CO2 inventory. Some of the oldest meta-sedimentary rocks found on Earth contain abundant carbonate from which impact-induced release of CO2 could have played a role in the formation and evolution of the atmosphere. Carbonates are also present in the target materials for approx. 30% of all terrestrial impact structures including large impacts such as Chicxulub which happened to occur at a location with extraordinarily thick platform carbonate 3-6 km deep. The impact release of CO2 from carbonates can cause global warming as a result of the well-known greenhouse effect and have subsequent effects on climate and biota. Therefore, the shock behavior of calcite is important in understanding the Cretaceous-Paleogene event and other impacts with carbonate-bearing sediments in their target(s) such as Mars and some asteroids. A comprehensive survey utilizing a variety of techniques to characterize the effects manifest in Calcite (Iceland Spar) experimentally shocked to 60.8 GPa has been completed. Results of analysis by Raman Spectroscopy are reported here.

  4. Dynamic Characterization of Mock Explosive Material Using Reverse Taylor Impact Experiments

    SciTech Connect

    Ferranti, L; Gagliardi, F J; Cunningham, B J; Vandersall, K S

    2010-03-25

    The motivation for the current study is to evaluate the dynamic loading response of an inert mock explosive material used to replicate the physical and mechanical properties of LX-17-1 and PBX 9502 insensitive high explosives. The evaluation of dynamic material parameters is needed for predicting the deformation behavior including the onset of failure and intensity of fragmentation resulting from high velocity impact events. These parameters are necessary for developing and validating physically based material constitutive models that will characterize the safety and performance of energetic materials. The preliminary study uses a reverse Taylor impact configuration that was designed to measure the dynamic behavior of the explosive mock up to and including associated fragmentation. A stationary rod-shaped specimen was impacted using a compressed-gas gun by accelerating a rigid steel anvil attached to a sabot. The impact test employed high-speed imaging and velocity interferometry diagnostics for capturing the transient deformation of the sample at discrete times. Once established as a viable experimental technique with mock explosives, future studies will examine the dynamic response of insensitive high explosives and propellants.

  5. Preparation and characterization of {sup 238}Pu-ceramics for radiation damage experiments

    SciTech Connect

    DM Strachan; RD Scheele; WC Buchmiller; JD Vienna; RL Sell; RJ Elovich

    2000-06-15

    become metamict and the damage saturates. They will characterize and test these specimens every 6 months by (1) monitoring the dimensions, (2) monitoring the geometric and pycnometric densities, (3) monitoring the appearance, (4) determining the normalized amount leached during a 3-day, static, 90 C leach test in high purity water, and (5) monitoring the crystal structure with x-ray diffraction crystallography (XRD). In this paper, the authors document the preparation and initial characterization of the materials that were made in this study. The initial XRD characterizations indicate that the phase assemblages appear to be correct with the exception of the {sup 238}Pu-zirconolite baseline material. They made this latter material using too much Pu, so this material contains unreacted PuO{sub 2}. The characterization of the physical properties of these materials found that the densities for all but three materials appear to be > 94% of theoretical, and only a few of the specimens have significant cracking. Those with cracking were the {sup 239}Pu-zirconolite specimens, which were sintered with a heat-up rate of 5 C/min. They sintered the {sup 238}Pu-zirconolite specimens with a heat-up rate of 2.5 C/min and obtained specimens with only minor surface cracking. Elemental releases during the 3-day MCC leach tests show that the normalized elemental releases depend on (1) whether the Pu is {sup 239}Pu or {sup 238}Pu, (2) the material type, and (3) the identity of the constituent. The effect of the Pu isotope in the ceramic is most dramatic for Pu release, with nominally 50 to 100 times more Pu activity released from the {sup 238}Pu specimens. This is unlikely to be an early indicator of radiation damage, because of the short time between specimen preparation and testing. In contrast greater amounts of Mo are released from the {sup 239}Pu specimens. Of the contained constituents, Ca Al, Pu, and U are the species found at relatively higher levels in the leachates.

  6. Hydrogeological characterization of a bank filtration experiment site at the Rio Grande, El Paso, Texas, USA

    NASA Astrophysics Data System (ADS)

    Langford, R.; Schulze-Makuch, D.; Pillai, S.; Abdel-Fattah, A.; Widmer, K.

    2003-04-01

    An experiment site was constructed along an artificial channel of the Rio Grande in El Paso, Texas. The experiment was funded by the EPA and is designed to measure the effectiveness of bank filtration in an arid environment. Regionally, the experiment is important because of the hundreds of thousands of people drinking water from shallow wells drilled in close proximity to septic systems. A pumping well was drilled 17 meters from the stream bank and screened from 3.5 to 8 m depth. A cruciform array of observation wells with several multilevel completions allows detection of downstream and vertical movement of water as well as flow from the stream to the well. All of the wells were continuously cored during drilling. Analysis of the cores reveals that the site consists of two stacked channels filled with sand deposited from the meandering Rio Grande. A grid of ground-penetrating radar lines provided three-dimensional coverage between wells and showed bedding to 6.5 m depth. Constant head hydraulic conductivities show that the aquifer consists of two more permeable units separated by the less permeable upper fill of the lower channel complex, with vertical hydraulic conductivities of (1x10-6 to 2x10-6 m/s?). The intervals above and below this interval have the highest vertical conductivities (up to 3.5x10-5 m/s). A multiple pumping and tracer test was conducted using the cruciform array of the field site that consisted of a pumping well, 16 observation wells, and a stream sampling point. The average hydraulic conductivity of the geological media at the field site was about 2 x 10-3 m/s based on pumping test analysis. However, the type curve responses revealed significant heterogeneity of hydraulic conductivity throughout the field site. For the tracer test, bromide and microspheres were used as tracers. Microspheres were used to mimic the behavior of Giardia and Cryptosporidium. The tracers (bromide and microspheres of different sizes and colors) were injected in one

  7. Characterization of Sodium Ion Electrochemical Reaction with Tin Anodes: Experiment and Theory

    SciTech Connect

    Baggetto, Loic; Meisner, Roberta A; Ganesh, Panchapakesan; Unocic, Raymond R; Bridges, Craig A; Jumas, Dr. Jean-Claude; Veith, Gabriel M

    2013-01-01

    Tin anodes show a rich structure and reaction chemistry which we have investigated in detail. Upon discharge five plateaus are observed corresponding to -Sn, an unidentified phase (Na/Sn = 0.6), an amorphous phase (Na/Sn = 1.2), a hexagonal R-3m Na5Sn2, and fully sodiated Na15Sn 4. With charging there are six plateaus, which are related to the formation of Na5Sn2 followed by the formation of amorphous phases and -Sn. Upon cycling the formation of metastable Na5Sn2 seems to be suppressed, leading to a single charge plateau at 0.2 V. Theoretical voltages calculated from existing crystal structures using DFT provide a good match with constant current measurements, however, the voltage is more negative compared to quasi-equilibrium measurements (GITT). Search for additional (meta)stable phases using cluster-expansion method predicts many phases lower in energy than the convex hull, including the R-3m Na5Sn2 phase characterized experimentally. The presence of multiple phases in varying lattices with very similar formation energy suggests why the reaction mechanism is non-reversible. Interpretation of M ssbauer spectroscopy data is not yet elucidated due to the very low recoil-free fraction of the materials. The electrode surface is terminated with a SEI layer rich in carbonates such as Na2CO3 and Na alkyl carbonates as evidenced by XPS. After a full charge at 2V, strong evidence for the formation of oxidized Sn4+ is obtained. Subjecting the electrode to a rest after a full charge at 2 V reveals that aging in the electrolyte reduces the oxidized Sn4+ into Sn2+ and Sn0, and concomitantly suppresses the electrolyte decomposition represented by an anomalous discharge plateau at 1.2 V. Thereby, the catalytic decomposition of the electrolyte during discharge is caused by nanosized Sn particles covered by oxidized Sn4+ and not by pure metallic Sn.

  8. Characterizing the impact of urban emissions on regional aerosol particles: airborne measurements during the MEGAPOLI experiment

    NASA Astrophysics Data System (ADS)

    Freney, E. J.; Sellegri, K.; Canonaco, F.; Colomb, A.; Borbon, A.; Michoud, V.; Doussin, J.-F.; Crumeyrolle, S.; Amarouche, N.; Pichon, J.-M.; Bourianne, T.; Gomes, L.; Prevot, A. S. H.; Beekmann, M.; Schwarzenböeck, A.

    2014-02-01

    The MEGAPOLI (Megacities: Emissions, urban, regional and Global Atmospheric POLlution and climate effects, and Integrated tools for assessment and mitigation) experiment took place in July 2009. The aim of this campaign was to study the aging and reactions of aerosol and gas-phase emissions in the city of Paris. Three ground-based measurement sites and several mobile platforms including instrument equipped vehicles and the ATR-42 aircraft were involved. We present here the variations in particle- and gas-phase species over the city of Paris, using a combination of high-time resolution measurements aboard the ATR-42 aircraft. Particle chemical composition was measured using a compact time-of-flight aerosol mass spectrometer (C-ToF-AMS), giving detailed information on the non-refractory submicron aerosol species. The mass concentration of black carbon (BC), measured by a particle absorption soot photometer (PSAP), was used as a marker to identify the urban pollution plume boundaries. Aerosol mass concentrations and composition were affected by air-mass history, with air masses that spent longest time over land having highest fractions of organic aerosol and higher total mass concentrations. The Paris plume is mainly composed of organic aerosol (OA), BC, and nitrate aerosol, as well as high concentrations of anthropogenic gas-phase species such as toluene, benzene, and NOx. Using BC and CO as tracers for air-mass dilution, we observe the ratio of ΔOA / ΔBC and ΔOA / ΔCO increase with increasing photochemical age (-log(NOx / NOy)). Plotting the equivalent ratios of different organic aerosol species (LV-OOA, SV-OOA, and HOA) illustrate that the increase in OA is a result of secondary organic aerosol (SOA) formation. Within Paris the changes in the ΔOA / ΔCO are similar to those observed during other studies in London, Mexico City, and in New England, USA. Using the measured SOA volatile organic compounds (VOCs) species together with organic aerosol formation

  9. Characterizing the impact of urban emissions on regional aerosol particles; airborne measurements during the MEGAPOLI experiment

    NASA Astrophysics Data System (ADS)

    Freney, E. J.; Sellegri, K.; Canonaco, F.; Colomb, A.; Borbon, A.; Michoud, V.; Doussin, J.-F.; Crumeyrolle, S.; Amarouch, N.; Pichon, J.-M.; Prévôt, A. S. H.; Beekmann, M.; Schwarzenböeck, A.

    2013-09-01

    The MEGAPOLI experiment took place in July 2009. The aim of this campaign was to study the aging and reactions of aerosol and gas-phase emissions in the city of Paris. Three ground-based measurement sites and several mobile platforms including instrument equipped vehicles and the ATR-42 aircraft were involved. We present here the variations in particle- and gas-phase species over the city of Paris using a combination of high-time resolution measurements aboard the ATR-42 aircraft. Particle chemical composition was measured using a compact time-of-flight aerosol mass spectrometer (C-ToF-AMS) giving detailed information of the non-refractory submicron aerosol species. The mass concentration of BC, measured by a particle absorption soot photometer (PSAP), was used as a marker to identify the urban pollution plume boundaries. Aerosol mass concentrations and composition were affected by air-mass history, with air masses that spent longest time over land having highest fractions of organic aerosol and higher total mass concentrations. The Paris plume is mainly composed of organic aerosol (OA), black carbon and nitrate aerosol, as well as high concentrations of anthropogenic gas-phase species such as toluene, benzene, and NOx. Using BC and CO as tracers for air-mass dilution, we observe the ratio of ΔOA / ΔBC and ΔOA / ΔCO increase with increasing photochemical age (-log(NOx / NOy). Plotting the equivalent ratios for the Positive Matrix Factorization (PMF) resolved species (LV-OOA, SV-OOA, and HOA) illustrate that the increase in OA is a result of secondary organic aerosol (SOA). Within Paris the changes in the ΔOA / ΔCO are similar to those observed during other studies in Mexico city, Mexico and in New England, USA. Using the measured VOCs species together with recent organic aerosol formation yields we predicted ~ 50% of the measured organics. These airborne measurements during the MEGAPOLI experiment show that urban emissions contribute to the formation of OA

  10. Evaluation of fiber optic distributed temperature sensing in characterization of borehole fractures: a laboratory experiment

    NASA Astrophysics Data System (ADS)

    Roshan, Hamid; Queen, Gabriella; Andersen, Martin S.; Acworth, Ian R.

    2014-05-01

    Mapping of bedrock fractures in boreholes and the contribution of main fractures to groundwater flow have long been a significant challenge in the geosciences field. Advanced techniques such as formation micro-imager (FMI) are able to detect the location of downhole fractures and to characterise their properties, such as aperture and orientation. However, these techniques have not been designed to estimate flow from individual fractures and are, in many cases, economically unjustified. In recent years, Fiber Optic Distributed Temperature Sensing (DTS) has been used to detect the location of active fractures and their contribution to groundwater flow, however; the technique has not been evaluated in a controlled environment and the limitations of the technique have yet to be identified. For that reason, a fractured rock borehole with active fractures was simulated in a lab-scale experiment. A structure with two fractures was built in a cylindrical configuration around the borehole and placed inside a cylindrical reservoir. A coiled fibre optic cable was inserted in the centre of the borehole. In order to simulate groundwater interactions, water with distinct temperature was added to the reservoir. During tests, water from the borehole in the centre was pumped out of the system, while the fiber optic DTS recorded the temperature response. The location of the artificial fractures and their contribution to the flow rate were determined through analysis of the measured temperature data. The results show that for the experimental setup, the locations of the fractures are most easily detected from the early times of the temperature response. As the water with different temperature from the reservoir flows into the borehole, it changes the borehole temperature starting from around the fracture locations. With time, this anomaly disappears and the borehole temperature reaches a new steady state condition. The contribution of each fracture to the pumping flow can then be

  11. Final Report One-Twelfth-Scale Mixing Experiments to Characterize Double-Shell Tank Slurry Uniformity

    SciTech Connect

    Bamberger, Judith A.; Liljegren, Lucia M.; Enderlin, Carl W.; Meyer, Perry A.; Greenwood, Margaret S.; Titzler, Patricia A.; Terrones, Guillermo

    2007-09-01

    The objectives of these 1/12-scale scoping experiments were to: Determine which of the dimensionless parameters discussed in Bamberger and Liljegren (1994) affect the maximum concentration that can be suspended during jet mixer pump operation in the full-scale double-shell tanks; Develop empirical correlations to predict the nozzle velocity required for jet mixer pumps to suspend the contents of full-scale double-shell tanks; Apply the models to predict the nozzle velocity required to suspend the contents of Tank 241 AZ-101; Obtain experimental concentration data to compare with the TEMPEST( )(Trent and Eyler 1989) computational modeling predictions to guide further code development; Analyze the effects of changing nozzle diameter on exit velocity (U0) and U0D0 (the product of the exit velocity and nozzle diameter) required to suspend the contents of a tank. The scoping study experimentally evaluated uniformity in a 1/12-scale experiment varying the Reynolds number, Froude number, and gravitational settling parameter space. The initial matrix specified only tests at 100% U0D0 and 25% U0D0. After initial tests were conducted with small diameter, low viscosity simulant this matrix was revised to allow evaluation of a broader range of U0D0s. The revised matrix included full factorial test between 100% and 50% U0D0 and two half-factorial tests at 75% and 25% U0D0. Adding points at 75% U0D0 and 50% U0D0 allowed evaluation curvature. Eliminating points at 25% U0D0 decreased the testing time by several weeks. Test conditions were achieved by varying the simulant viscosity, the mean particle size, and the jet nozzle exit velocity. Concentration measurements at sampling locations throughout the tank were used to assess the degree of uniformity achieved during each test. Concentration data was obtained using a real time ultrasonic attenuation probe and discrete batch samples. The undissolved solids concentration at these locations was analyzed to determine whether the tank

  12. Characterization of Disruption Halo Currents in the National Spherical Torus Experiment

    SciTech Connect

    S.P. Gerhardt, J. Menard, S. Sabbagh and F. Scotti

    2012-04-25

    This paper describes the general characteristics of disruptions halo currents in the National Spherical Torus Experiment [M. Ono, et al. Nuclear Fusion 40, 557 (2000)]. The commonly observed types of vertical motion and resulting halo current patterns are described, and it is shown that plasma discharges developing between components can facilitate halo current flow. The halo current fractions and toroidal peaking factors at various locations in the device are presented. The maximum product of these two metrics for localized halo current measurements is always significantly less than the worst-case expectations from conventional aspect ratio tokamaks (which are typically written in terms of the total halo current). The halo current fraction and impulse is often largest in cases with the fastest plasma current quenches and highest quench rates. The effective duration of the halo current pulse is comparable to or shorter than the plasma current quench time. The largest halo currents have tended to occur in lower β and lower elongation plasmas. The sign of the poloidal halo current is reversed when the toroidal field direction is reversed.

  13. Characterization of dynamic stall on 9-15 % thick airfoils using experiment and computation

    NASA Astrophysics Data System (ADS)

    Davidson, Phillip B.

    In recent years, the blade geometry on wind turbines and helicopters has been optimized for a particular span location. Unsteady flow phenomena like dynamic stall limit these designs and need to be better understood and correctly simulated. Currently, empirical and computational fluid dynamics (CFD) methods are used to simulate rotating wind turbine or helicopter blades, but each of these methods has limitations in predicting unsteady separated flows. To address these needs, the present work investigated oscillating airfoils over a range of conditions with an approach that provided fast, low-cost unsteady pressure data combined with a highly resolved flow field to better understand the physics of dynamic stall. An additional objective was to show how such data may be used to assess CFD simulations. This research has yielded interesting results showing characteristics of thin airfoil stall, leading edge stall, and trailing edge stall that were sorted and classified. Classification of the oscillating airfoil behavior with or without dynamic stall was performed using previous definitions for stall regime, separation characteristics, and other qualitative differences in stall pattern. After classifying the unsteady flow for each of the cases, comparison of experimental results and results obtained using an unsteady Reynolds Averaged Navier-Stokes (URANS) solver was performed to assess the ability of the solver to produce the same unsteady effects. Although both experiment and computation produced similar flow features, the timing and magnitude of the features in the dynamic stall and re-attachment process of the pitching cycle exhibited some significant differences.

  14. Characterizing biogeochemical processes in the hyporheic zone using flume experiments and reactive transport modeling

    NASA Astrophysics Data System (ADS)

    Quick, A. M.; Reeder, W. J.; Farrell, T. B.; Feris, K. P.; Tonina, D.; Benner, S. G.

    2015-12-01

    The hyporheic zones of streams are hotspots of biogeochemical cycling, where reactants from surface water and groundwater are continually brought into contact with microbial populations on the surfaces of stream sediments and reaction products are removed by hyporheic flow and degassing. Using large flume experiments we have documented the complex redox dynamics associated with dune-scale hyporheic flow. Observations, coupled with reactive transport modeling, provide insight into how flow dictates spatio-temporal distribution of redox reactions and the associated consumption and production of reactants and products. Dune hyporheic flow was experimentally produced by maintaining control over flow rates, slopes, sediment grain size, bedform geomorphology, and organic carbon content. An extensive in-situ monitoring array combined with sampling events over time elucidated redox-sensitive processes including constraints on the spatial distribution and magnitude of aerobic respiration, organic carbon consumption, sulfide deposition, and denitrification. Reactive transport modeling reveals further insight into the influence of system geometry and reaction rate. As an example application of the model, the relationship between residence times and reaction rates may be used to generate Damköhler numbers that are related to biogeochemical processes, such as the potential of streambed morphology and nitrate loading to influence production of the greenhouse gas nitrous oxide via incomplete denitrification.

  15. Characterization of the plasma current quench during disruptions in the National Spherical Torus Experiment

    SciTech Connect

    Gerhardt, S.P., Menard, J.E., and the NSTX Research Team

    2008-12-17

    A detailed analysis of the plasma current quench in the National Spherical Torus Experiment [M.Ono, et al Nuclear Fusion 40, 557 (2000)] is presented. The fastest current quenches are fit better by a linear waveform than an exponential one. Area-normalized current quench times down to .4 msec/m2 have been observed, compared to the minimum of 1.7 msec/m2 recommendation based on conventional aspect ratio tokamaks; as noted in previous ITPA studies, the difference can be explained by the reduced self-inductance at low aspect ratio and high-elongation. The maximum instantaneous dIp/dt is often many times larger than the mean quench rate, and the plasma current before the disruption is often substantially less than the flat-top value. The poloidal field time-derivative during the disruption, which is directly responsible for driving eddy currents, has been recorded at various locations around the vessel. The Ip quench rate, plasma motion, and magnetic geometry all play important roles in determining the rate of poloidal field change.

  16. Characterizing the Response of Composite Panels to a Pyroshock Induced Environment Using Design of Experiments Methodology

    NASA Technical Reports Server (NTRS)

    Parsons, David S.; Ordway, David; Johnson, Kenneth

    2013-01-01

    This experimental study seeks to quantify the impact various composite parameters have on the structural response of a composite structure in a pyroshock environment. The prediction of an aerospace structure's response to pyroshock induced loading is largely dependent on empirical databases created from collections of development and flight test data. While there is significant structural response data due to pyroshock induced loading for metallic structures, there is much less data available for composite structures. One challenge of developing a composite pyroshock response database as well as empirical prediction methods for composite structures is the large number of parameters associated with composite materials. This experimental study uses data from a test series planned using design of experiments (DOE) methods. Statistical analysis methods are then used to identify which composite material parameters most greatly influence a flat composite panel's structural response to pyroshock induced loading. The parameters considered are panel thickness, type of ply, ply orientation, and pyroshock level induced into the panel. The results of this test will aid in future large scale testing by eliminating insignificant parameters as well as aid in the development of empirical scaling methods for composite structures' response to pyroshock induced loading.

  17. Characterizing the Response of Composite Panels to a Pyroshock Induced Environment using Design of Experiments Methodology

    NASA Technical Reports Server (NTRS)

    Parsons, David S.; Ordway, David O.; Johnson, Kenneth L.

    2013-01-01

    This experimental study seeks to quantify the impact various composite parameters have on the structural response of a composite structure in a pyroshock environment. The prediction of an aerospace structure's response to pyroshock induced loading is largely dependent on empirical databases created from collections of development and flight test data. While there is significant structural response data due to pyroshock induced loading for metallic structures, there is much less data available for composite structures. One challenge of developing a composite pyroshock response database as well as empirical prediction methods for composite structures is the large number of parameters associated with composite materials. This experimental study uses data from a test series planned using design of experiments (DOE) methods. Statistical analysis methods are then used to identify which composite material parameters most greatly influence a flat composite panel's structural response to pyroshock induced loading. The parameters considered are panel thickness, type of ply, ply orientation, and pyroshock level induced into the panel. The results of this test will aid in future large scale testing by eliminating insignificant parameters as well as aid in the development of empirical scaling methods for composite structures' response to pyroshock induced loading.

  18. Characterizing the Response of Composite Panels to a Pyroshock Induced Environment Using Design of Experiments Methodology

    NASA Technical Reports Server (NTRS)

    Parsons, David; Ordway, David; Johnson, Kenneth

    2013-01-01

    This experimental study seeks to quantify the impact various composite parameters have on the structural response of a composite structure in a pyroshock environment. The prediction of an aerospace structure's response to shock induced loading is largely dependent on empirical databases created from collections of development and flight test data. While there is significant structural response data due to shock induced loading for metallic structures, there is much less data available for composite structures. One challenge of developing a composite shock response database as well as empirical prediction methods for composite structures is the large number of parameters associated with composite materials. This experimental study uses data from a test series planned using design of experiments (DOE) methods. Statistical analysis methods are then used to identify which composite material parameters most greatly influence a flat composite panel's structural response to shock induced loading. The parameters considered are panel thickness, type of ply, ply orientation, and shock level induced into the panel. The results of this test will aid in future large scale testing by eliminating insignificant parameters as well as aid in the development of empirical scaling methods for composite structures' response to shock induced loading.

  19. Novel Feed-through Richtmyer-Meshkov Instability (RMI) Experiment for Characterization of Dynamic Material Response

    NASA Astrophysics Data System (ADS)

    Opie, Saul; Gautam, Sudrishti; Fortin, Elizabeth; Lynch, Jenna; Loomis, Eric; Peralta, Pedro

    Hydrodynamic instabilities occur often in applications where forces act across a bimaterial interface. In Rayleigh-Taylor (RT) instabilities, surface perturbations grow exponentially under opposing pressure and density gradients. In the closely related Richtmyer-Meshkov (RM) instability, the same perturbations grow linearly due to an impulsive acceleration, e.g., a passing shock wave. These effects are often analyzed with linear fluid theory, but it is well known that for materials possessing shear strength the perturbation evolution can be significantly affected. A challenge in modeling these effects is that existing knowledge of the interplay between strength and hydrodynamic instabilities in solids is limited for the loads and strain rates that are typically used to study them. We have developed novel feed-through RM instability experiments that are useful to understand and model this interplay. We will describe the experimental setup and show simulations that agree well with experimental results in two materials, one-phase copper, and iron loaded above and below the alpha-epsilon phase boundary, where modeling used a phase-aware strength model. In copper, the growth of surface perturbations is quite sensitive to strength model parameters, and so is the amplitude of the shock front perturbations. This is also observed in iron, along with an additional sensitivity in the modeling results to the parameters used to describe phase change kinetics. Work supported by Department of Energy (DOE) [Grant Number DE-SC0008683] from the Office of Fusion Energy Science.

  20. Characterization of titanium films in the Tandem Mirror Experiment Upgrade (TMX-U)

    SciTech Connect

    Hsu, W.L.; Bastasz, R.; Bauer, W.; Malinowski, M.E.; Mills, B.E.; Allen, S.L.; Clower, C.A.

    1984-04-01

    Titanium gettering is used extensively in the Tandem Mirror Experiment Upgrade (TMX-U) to achieve good vacuum conditions for the production of a sustained energetic plasma. The gettering films under different operating conditions were analyzed by thermal desorption spectroscopy and Auger electron spectroscopy. A sputter profile of the films showed a Ti deposition rate of approx. =1 nm per evaporation sequence. Large quantities of protium (H/Tiroughly-equal0.2), oxygen (O/Tiroughly-equal0.3), and carbon (C/Tiroughly-equal0.1) were detected in the films regardless of their location and the plasma isotope to which they were exposed. While the Ti films that were exposed to deuterium plasmas showed a substantial increase in deuterium content, deuterium still remained a small fraction of the overall hydrogen concentration (D/Hroughly-equal0.1). Analyses of the results suggest that getter pumping of H/sub 2/O from the background gas in the (TMX-U) vacuum system is the primary source for protium and oxygen measured in the films.

  1. 1/12-Scale scoping experiments to characterize double-shell tank slurry uniformity: Test plan

    SciTech Connect

    Bamberger, J.A.; Liljegren, L.M.

    1994-10-01

    Million gallon double-shell tanks (DSTs) at Hanford are used to store transuranic, high-level, and low-level wastes. These wastes generally consist of a large volume of salt-laden solution covering a smaller volume of settled sludge primarily containing metal hydroxides. These wastes will be retrieved and processed into immobile waste forms suitable for permanent disposal. The current retrieval concept is to use submerged dual-nozzle pumps to mobilize the settled solids by creating jets of fluid that are directed at the tank solids. The pumps oscillate, creating arcs of high-velocity fluid jets that sweep the floor of the tank. After the solids are mobilized, the pumps will continue to operate at a reduced flow rate sufficient to maintain the particles in a uniform suspension. The objectives of these 1/12-scale scoping experiments are to determine how Reynolds number, Froude number, and gravitational settling parameter affect the degree of uniformity achieved during jet mixer pump operation in the full-scale double-shell tanks; develop linear models to predict the degree of uniformity achieved by jet mixer pumps operating in the full-scale double-shell tanks; apply linear models to predict the degree of uniformity that will be achieved in tank 241-AZ-101 and determine whether contents of that tank will be uniform to within {+-} 10% of the mean concentration; and obtain experimental concentration and jet velocity data to compared with the TEMPEST computational and modeling predictions to guide further code development.

  2. Synthesis and Characterization of Aldol Condensation Products from Unknown Aldehydes and Ketones: An Inquiry-Based Experiment in the Undergraduate Laboratory

    ERIC Educational Resources Information Center

    Angelo, Nicholas G.; Henchey, Laura K.; Waxman, Adam J.; Canary, James W.; Arora, Paramjit S.; Wink, Donald

    2007-01-01

    An experiment for the undergraduate chemistry laboratory in which students perform the aldol condensation on an unknown aldehyde and an unknown ketone is described. The experiment involves the use of techniques such as TLC, column chromatography, and recrystallization, and compounds are characterized by [to the first power]H NMR, GC-MS, and FTIR.…

  3. Expression, Purification, and Characterization of a Carbohydrate-Active Enzyme: A Research-Inspired Methods Optimization Experiment for the Biochemistry Laboratory

    ERIC Educational Resources Information Center

    Willbur, Jaime F.; Vail, Justin D.; Mitchell, Lindsey N.; Jakeman, David L.; Timmons, Shannon C.

    2016-01-01

    The development and implementation of research-inspired, discovery-based experiences into science laboratory curricula is a proven strategy for increasing student engagement and ownership of experiments. In the novel laboratory module described herein, students learn to express, purify, and characterize a carbohydrate-active enzyme using modern…

  4. Structure Characterization in Inhomogeneous Media by Means of Ultrasonic Scattering: Monte Carlo Methods and Experiments

    NASA Astrophysics Data System (ADS)

    Grolemund, Daniel Lee

    The dissertation concerns the extraction, via signal processing, of structural information from the scattering of low megahertz, low power ultrasonic waves in two specific media of great practical interest--fiber reinforced composites and soft biological tissue. In fiber reinforced composites, this work represents the first measurement of second-order statistics in porous laminates, and the first application of Monte Carlo methods to acoustical scattering in composites. A numerical model of porous composites backscatter was derived which is suitable for direct numerical implementation. The model treats the total backscattered field as the result of a two-mode scattering process. In the first mode, the void-free composite is treated as a continuously varying medium in which the density and compressibility are functions of position. The second mode is the distribution of gas voids that failed to escape the material before gel, and are dealt with as discrete Rayleigh scatterers. Convolution techniques were developed that allowed the numerical model to reproduce the long range order seen in the void-free composite. The results of the Monte Carlo derivation were coded, and simulations run with data sets that duplicate the properties of the composite samples used in the study. In the area of tissue characterization, two leading methods have been proposed to extract structural data from the raw backscattered waveforms. Both techniques were developed from an understanding of the periodicities created by semi-regularly spaced, coherent scatterers. In the second half of the dissertation, a complete analytical and numerical treatment of these two techniques was done from a first principles approach. Computer simulations were performed to determine the general behavior of the algorithms. The main focus is on the envelope correlation spectrum, or ECS. Monte Carlo methods were employed to examine the signal-to-noise ratio of the ECS in terms of the variances of the backscattered

  5. Characterization of small, Type V edge-localized modes in the National Spherical Torus Experiment

    SciTech Connect

    Maingi, Rajesh; Bell, M.; Fredrickson, E.; Lee, K. C.; Maqueda, R. J.; Snyder, P.; Tritz, K.; Zweben, S. J.; Bell, R. E.; Biewer, Theodore M; Bush, Charles E; Boedo, J.; Brooks, N. H.; Delgado-Aparicio, L.; Domier, C. W.; Gates, D.; Johnson, D. W.; Kaita, R.; Kaye, S. M.; Kugel, H.; LaBlanc, B. P.; Luhmann, N. C.; Menard, J.; Mueller, D.; Park, H.; Raman, R; Roquemore, A. L.; Sabbagh, S. A.; Soukhanovskii, V. A.; Stevenson, T.; Stutman, D.

    2006-01-01

    There has been a substantial international research effort in the fusion community to identify tokamak operating regimes with either small or no periodic bursts of particles and power from the edge plasma, known as edge-localized modes (ELMs). While several candidate regimes have been presented in the literature, very little has been published on the characteristics of the small ELMs themselves. One such small ELM regime, also known as the Type V ELM regime, was recently identified in the National Spherical Torus Experiment [M. Ono, S. M. Kaye, Y.-K. M. Peng et al., Nucl. Fusion 40, 557 (2000)]. In this paper, the spatial and temporal structure of the Type V ELMs is presented, as measured by several different diagnostics. The composite picture of the Type V ELM is of an instability with one or two filaments that rotate toroidally at ~5-10 km/s, in the direction opposite to the plasma current and neutral beam injection. The toroidal extent of Type V ELMs is typically ~5 m, whereas the cross-field (radial) extent is typically 10 cm (3cm), yielding a portrait of an electromagnetic, ribbon-like perturbation aligned with the total magnetic field. The filaments comprising the Type V ELM appear to be destabilized near the top of the H-mode pedestal and drift radially outward as they rotate toroidally. After the filaments come in contact with the open field lines, the divertor plasma perturbations are qualitatively similar to other ELM types, albeit with only one or two filaments in the Type V ELM versus more filaments for Type I and Type III ELMs. Preliminary stability calculations eliminate pressure driven modes as the underlying instability for Type V ELMs, but more work is required to determine if current driven modes are responsible for destabilization.

  6. Characterization of small, Type V edge-localized modes in the National Spherical Torus Experiment

    SciTech Connect

    Maingi, R.; Biewer, T. M.; Bush, C. E.; Bell, M. G.; Fredrickson, E. D.; Zweben, S. J.; Bell, R. E.; Gates, D. A.; Johnson, D. W.; Kaita, R.; Kaye, S. M.; Kugel, H. W.; LeBlanc, B. P.; Menard, J. E.; Mueller, D.; Park, H.; Roquemore, A. L.; Stevenson, T.; Lee, K. C.; Domier, C. W.

    2006-09-15

    There has been a substantial international research effort in the fusion community to identify tokamak operating regimes with either small or no periodic bursts of particles and power from the edge plasma, known as edge-localized modes (ELMs). While several candidate regimes have been presented in the literature, very little has been published on the characteristics of the small ELMs themselves. One such small ELM regime, also known as the Type V ELM regime, was recently identified in the National Spherical Torus Experiment [M. Ono, S. M. Kaye, Y.-K. M. Peng et al., Nucl. Fusion 40, 557 (2000)]. In this paper, the spatial and temporal structure of the Type V ELMs is presented, as measured by several different diagnostics. The composite picture of the Type V ELM is of an instability with one or two filaments that rotate toroidally at {approx}5-10 km/s, in the direction opposite to the plasma current and neutral beam injection. The toroidal extent of Type V ELMs is typically {approx}5 m, whereas the cross-field (radial) extent is typically {approx}10 cm (3 cm), yielding a portrait of an electromagnetic, ribbon-like perturbation aligned with the total magnetic field. The filaments comprising the Type V ELM appear to be destabilized near the top of the H-mode pedestal and drift radially outward as they rotate toroidally. After the filaments come in contact with the open field lines, the divertor plasma perturbations are qualitatively similar to other ELM types, albeit with only one or two filaments in the Type V ELM versus more filaments for Type I or Type III ELMs. Preliminary stability calculations eliminate pressure driven modes as the underlying instability for Type V ELMs, but more work is required to determine if current driven modes are responsible for destabilization.

  7. Experimental characterization of a coaxial plasma accelerator for a colliding plasma experiment

    NASA Astrophysics Data System (ADS)

    Wiechula, J.; Hock, C.; Iberler, M.; Manegold, T.; Schönlein, A.; Jacoby, J.

    2015-04-01

    We report experimental results of a single coaxial plasma accelerator in preparation for a colliding plasma experiment. The utilized device consisted of a coaxial pair of electrodes, accelerating the plasma due to J ×B forces. A pulse forming network, composed of three capacitors connected in parallel, with a total capacitance of 27 μF was set up. A thyratron allowed to switch the maximum applied voltage of 9 kV. Under these conditions, the pulsed currents reached peak values of about 103 kA. The measurements were performed in a small vacuum chamber with a neutral-gas prefill at gas pressures between 10 Pa and 14 000 Pa. A gas mixture of ArH2 with 2.8% H2 served as the discharge medium. H2 was chosen in order to observe the broadening of the Hβ emission line and thus estimate the electron density. The electron density for a single plasma accelerator reached peak values on the order of 1016 cm-3 . Electrical parameters, inter alia inductance and resistance, were determined for the LCR circuit during the plasma acceleration as well as in a short circuit case. Depending on the applied voltage, the inductance and resistance reached values ranging from 194 nH to 216 nH and 13 mΩ to 23 mΩ, respectively. Furthermore, the plasma velocity was measured using a fast CCD camera. Plasma velocities of 2 km/s up to 17 km/s were observed, the magnitude being highly correlated with gas pressure and applied voltage.

  8. Experimental characterization of a coaxial plasma accelerator for a colliding plasma experiment

    SciTech Connect

    Wiechula, J.; Hock, C.; Iberler, M.; Manegold, T.; Schönlein, A.; Jacoby, J.

    2015-04-15

    We report experimental results of a single coaxial plasma accelerator in preparation for a colliding plasma experiment. The utilized device consisted of a coaxial pair of electrodes, accelerating the plasma due to J×B forces. A pulse forming network, composed of three capacitors connected in parallel, with a total capacitance of 27 μF was set up. A thyratron allowed to switch the maximum applied voltage of 9 kV. Under these conditions, the pulsed currents reached peak values of about 103 kA. The measurements were performed in a small vacuum chamber with a neutral-gas prefill at gas pressures between 10 Pa and 14 000 Pa. A gas mixture of ArH{sub 2} with 2.8% H{sub 2} served as the discharge medium. H{sub 2} was chosen in order to observe the broadening of the H{sub β} emission line and thus estimate the electron density. The electron density for a single plasma accelerator reached peak values on the order of 10{sup 16} cm{sup −3}. Electrical parameters, inter alia inductance and resistance, were determined for the LCR circuit during the plasma acceleration as well as in a short circuit case. Depending on the applied voltage, the inductance and resistance reached values ranging from 194 nH to 216 nH and 13 mΩ to 23 mΩ, respectively. Furthermore, the plasma velocity was measured using a fast CCD camera. Plasma velocities of 2 km/s up to 17 km/s were observed, the magnitude being highly correlated with gas pressure and applied voltage.

  9. Rural continental aerosol properties and processes observed during the Hohenpeissenberg Aerosol Characterization Experiment (HAZE2002)

    NASA Astrophysics Data System (ADS)

    Hock, N.; Schneider, J.; Borrmann, S.; Römpp, A.; Moortgat, G.; Franze, T.; Schauer, C.; Pöschl, U.; Plass-Dülmer, C.; Berresheim, H.

    2008-02-01

    Detailed investigations of the chemical and microphysical properties of rural continental aerosols were performed during the HAZE2002 experiment, which was conducted in May 2002 at the Meteorological Observatory Hohenpeissenberg (DWD) in Southern Germany. Online measurements included: Size-resolved chemical composition of submicron particles; total particle number concentrations and size distributions over the diameter range of 3 nm to 9 μm; gas-phase concentration of monoterpenes, CO, O3, OH, and H2SO4. Filter sampling and offline analytical techniques were used to determine: Fine particle mass (PM2.5), organic, elemental and total carbon in PM2.5 (OC2.5, EC2.5, TC2.5), and selected organic compounds (dicarboxylic acids, polycyclic aromatic hydrocarbons, proteins). Overall, the non-refractory components of submicron particles detected by aerosol mass spectrometry (PM1, 6.6±5.4 μg m-3, arithmetic mean and standard deviation) accounted for ~62% of PM2.5 determined by filter gravimetry (10.6±4.7 μg m-3). The relative proportions of non-refractory submicron particle components were: (23±39)% ammonium nitrate, (27±23)% ammonium sulfate, and (50±40)% organics (OM1). OM1 was closely correlated with PM1 (r2=0.9) indicating a near-constant ratio of non-refractory organics and inorganics. The average ratio of OM1 to OC2.5 was 2.1±1.4, indicating a high proportion of heteroelements in the organic fraction of the sampled rural aerosol. This is consistent with the high ratio of oxygenated organic aerosol (OOA) over hydrocarbon-like organic aerosol (HOA) inferred from the AMS results (4:1), and also with the high abundance of proteins (~3%) indicating a high proportion of primary biological material (~30%) in PM2.5. This finding was confirmed by low abundance of PAHs (<1 ng m-3) and EC (<1 μg m-3) in PM2.5 and detection of several secondary organic aerosol compounds (dicarboxylic acids) and their precursors (monoterpenes). New particle formation was observed almost

  10. Thermoplastic pultrusion development and characterization of residual in pultruded composites with modeling and experiments

    NASA Astrophysics Data System (ADS)

    Jamiyanaa, Khongor

    unbalanced layup can produce asymmetrical residual stress through the thickness and causes the part to bow. Furthermore, the residual stress through the thickness was mapped with excellent accuracy. A design of experiments around the processing parameters indicated that increase in pull speed or decrease in die temperature increased the residual stress within the part.

  11. Aerosol Composition, Chemistry, and Source Characterization during the 2008 VOCALS Experiment

    NASA Astrophysics Data System (ADS)

    Lee, Y.; Springston, S.; Jayne, J. T.; Wang, J.; Senum, G.; Hubbe, J.; Alexander, L.; Brioude, J.; Spak, S.; Mena-Carrasco, M.; Kleinman, L. I.; Daum, P. H.

    2009-12-01

    Chemical composition of fine aerosol particles over the northern Chilean coastal waters was determined on board the US DOE G-1 aircraft during the VOCALS (VAMOS Ocean-Cloud-Atmosphere-Land Study) field experiment between October 16 and November 15, 2008. Chemical species determined included SO42-, NO3-, NH4+, and total organics (Org) using an Aerodyne Aerosol Mass Spectrometer, and SO42-, NO3-, NH4+, Na+, Cl-, CH3SO3-, Mg2+, Ca2+, and K+ using a particle-into-liquid sampler-ion chromatography technique. The results show the marine boundary layer (MBL) aerosol mass was dominated by non-sea-salt SO42- followed by Na+, Cl-, Org, NO3-, and NH4+, in decreasing importance; CH3SO3-, Ca2+, and K+ rarely exceeded their respective limits of detection. The SO42- aerosols were strongly acidic as the equivalent NH4+ to SO42- ratio was only ~0.25 on average. NaCl particles, presumably of sea-salt origin, showed chloride deficits but retained Cl- typically more than half the equivalency of Na+, and are believed to be externally mixed with the acidic sulfate aerosols. Nitrate was observed only on sea-salt particles, consistent with adsorption of HNO3 on non-acidic sea-salt aerosols, responsible partly for the Cl- deficit. Dust particles appeared to play a minor role judging from the small volume differences between that derived from the observed mass concentrations and that calculated based on particle size distributions. Because SO42- concentrations in the study domain were substantial (~0.5 - ~3 μg/m3) with a strong gradient (highest near the shore decreasing with distance from land), and the ocean-emitted dimethylsulfide and its unique oxidation product, CH3SO3-, were very low (i.e., ≤ 40 parts per trillion and <0.05 μg/m3, respectively), the observed SO42- aerosols are believed to be primarily of terrestrial origin. Back trajectory calculations indicate sulfur emissions from smelters and power plants along coastal regions of Peru and Chile are the main sources of these SO4

  12. Measuring spatial variability of vapor flux to characterize vadose-zone VOC sources: Flow-cell experiments

    NASA Astrophysics Data System (ADS)

    Mainhagu, J.; Morrison, C.; Truex, M.; Oostrom, M.; Brusseau, M. L.

    2014-10-01

    A method termed vapor-phase tomography has recently been proposed to characterize the distribution of volatile organic contaminant mass in vadose-zone source areas, and to measure associated three-dimensional distributions of local contaminant mass discharge. The method is based on measuring the spatial variability of vapor flux, and thus inherent to its effectiveness is the premise that the magnitudes and temporal variability of vapor concentrations measured at different monitoring points within the interrogated area will be a function of the geospatial positions of the points relative to the source location. A series of flow-cell experiments was conducted to evaluate this premise. A well-defined source zone was created by injection and extraction of a non-reactive gas (SF6). Spatial and temporal concentration distributions obtained from the tests were compared to simulations produced with a mathematical model describing advective and diffusive transport. Tests were conducted to characterize both areal and vertical components of the application. Decreases in concentration over time were observed for monitoring points located on the opposite side of the source zone from the local-extraction point, whereas increases were observed for monitoring points located between the local-extraction point and the source zone. The results illustrate that comparison of temporal concentration profiles obtained at various monitoring points gives a general indication of the source location with respect to the extraction and monitoring points.

  13. Measuring Spatial Variability of Vapor Flux to Characterize Vadose-zone VOC Sources: Flow-cell Experiments

    SciTech Connect

    Mainhagu, Jon; Morrison, C.; Truex, Michael J.; Oostrom, Martinus; Brusseau, Mark

    2014-08-05

    A method termed vapor-phase tomography has recently been proposed to characterize the distribution of volatile organic contaminant mass in vadose-zone source areas, and to measure associated three-dimensional distributions of local contaminant mass discharge. The method is based on measuring the spatial variability of vapor flux, and thus inherent to its effectiveness is the premise that the magnitudes and temporal variability of vapor concentrations measured at different monitoring points within the interrogated area will be a function of the geospatial positions of the points relative to the source location. A series of flow-cell experiments was conducted to evaluate this premise. A well-defined source zone was created by injection and extraction of a non-reactive gas (SF6). Spatial and temporal concentration distributions obtained from the tests were compared to simulations produced with a mathematical model describing advective and diffusive transport. Tests were conducted to characterize both areal and vertical components of the application. Decreases in concentration over time were observed for monitoring points located on the opposite side of the source zone from the local–extraction point, whereas increases were observed for monitoring points located between the local–extraction point and the source zone. We found that the results illustrate that comparison of temporal concentration profiles obtained at various monitoring points gives a general indication of the source location with respect to the extraction and monitoring points.

  14. Measuring Spatial Variability of Vapor Flux to Characterize Vadose-zone VOC Sources: Flow-cell Experiments

    DOE PAGESBeta

    Mainhagu, Jon; Morrison, C.; Truex, Michael J.; Oostrom, Martinus; Brusseau, Mark

    2014-08-05

    A method termed vapor-phase tomography has recently been proposed to characterize the distribution of volatile organic contaminant mass in vadose-zone source areas, and to measure associated three-dimensional distributions of local contaminant mass discharge. The method is based on measuring the spatial variability of vapor flux, and thus inherent to its effectiveness is the premise that the magnitudes and temporal variability of vapor concentrations measured at different monitoring points within the interrogated area will be a function of the geospatial positions of the points relative to the source location. A series of flow-cell experiments was conducted to evaluate this premise. Amore » well-defined source zone was created by injection and extraction of a non-reactive gas (SF6). Spatial and temporal concentration distributions obtained from the tests were compared to simulations produced with a mathematical model describing advective and diffusive transport. Tests were conducted to characterize both areal and vertical components of the application. Decreases in concentration over time were observed for monitoring points located on the opposite side of the source zone from the local–extraction point, whereas increases were observed for monitoring points located between the local–extraction point and the source zone. We found that the results illustrate that comparison of temporal concentration profiles obtained at various monitoring points gives a general indication of the source location with respect to the extraction and monitoring points.« less

  15. Measuring Spatial Variability of Vapor Flux to Characterize Vadose-zone VOC Sources: Flow-cell Experiments

    PubMed Central

    Morrison, C.; Truex, M.; Oostrom, M.; Brusseau, M.L.

    2014-01-01

    A method termed vapor-phase tomography has recently been proposed to characterize the distribution of volatile organic contaminant mass in vadose-zone source areas, and to measure associated three-dimensional distributions of local contaminant mass discharge. The method is based on measuring the spatial variability of vapor flux, and thus inherent to its effectiveness is the premise that the magnitudes and temporal variability of vapor concentrations measured at different monitoring points within the interrogated area will be a function of the geospatial positions of the points relative to the source location. A series of flow-cell experiments was conducted to evaluate this premise. A well-defined source zone was created by injection and extraction of a non-reactive gas (SF6). Spatial and temporal concentration distributions obtained from the tests were compared to simulations produced with a mathematical model describing advective and diffusive transport. Tests were conducted to characterize both areal and vertical components of the application. Decreases in concentration over time were observed for monitoring points located on the opposite side of the source zone from the local–extraction point, whereas increases were observed for monitoring points located between the local–extraction point and the source zone. The results illustrate that comparison of temporal concentration profiles obtained at various monitoring points gives a general indication of the source location with respect to the extraction and monitoring points. PMID:25171394

  16. Development and characterization of a time-, position- and energy-resolved x-ray diagnostic for PBFA II target experiments

    SciTech Connect

    Derzon, M.S.; Filuk, A.B.; Pantuso, J.; Dukart, R.J.; Olsen, R.; Barber, T.; Bernard, M.

    1992-12-31

    A time-, position- and energy-resolved soft x-ray (100--500 eV) diagnostic is being developed for PBFA II target experiments. The diagnostic provides measurements of hydrodynamic motion and thermal gradients in light-ion fusion targets. A slit-image of the source is imprinted onto thin sheets (20{mu}m) of organic scintillator to create a one-dimensional image. The scintillator light is then proximity-coupled to a linear array of fiber-optics that transports the light to a streak camera that is operated without an intensifier. The streak camera output is recorded on a charge-coupled-device (CCD) camera. We are characterizing the spatial and temporal resolutions of the systems. This is done by collecting data from as many as 90 individual fibers and correcting for variations in throughput and the effects of spatial resolution to roughly 5% standard deviation in their relative throughput. Spatial resolution of these systems at the source is approximately 0.4 mm. Timing resolution is nominally 2 ns and it is limited primarily by the scintillator response and dispersion in the 50-m-long fiber array. We describe the measurement techniques and the results of the characterization.

  17. Measuring spatial variability of vapor flux to characterize vadose-zone VOC sources: flow-cell experiments.

    PubMed

    Mainhagu, J; Morrison, C; Truex, M; Oostrom, M; Brusseau, M L

    2014-10-15

    A method termed vapor-phase tomography has recently been proposed to characterize the distribution of volatile organic contaminant mass in vadose-zone source areas, and to measure associated three-dimensional distributions of local contaminant mass discharge. The method is based on measuring the spatial variability of vapor flux, and thus inherent to its effectiveness is the premise that the magnitudes and temporal variability of vapor concentrations measured at different monitoring points within the interrogated area will be a function of the geospatial positions of the points relative to the source location. A series of flow-cell experiments was conducted to evaluate this premise. A well-defined source zone was created by injection and extraction of a non-reactive gas (SF6). Spatial and temporal concentration distributions obtained from the tests were compared to simulations produced with a mathematical model describing advective and diffusive transport. Tests were conducted to characterize both areal and vertical components of the application. Decreases in concentration over time were observed for monitoring points located on the opposite side of the source zone from the local-extraction point, whereas increases were observed for monitoring points located between the local-extraction point and the source zone. The results illustrate that comparison of temporal concentration profiles obtained at various monitoring points gives a general indication of the source location with respect to the extraction and monitoring points. PMID:25171394

  18. Measuring Spatial Variability of Vapor Flux to Characterize Vadose-zone VOC Sources: Flow-cell Experiments

    SciTech Connect

    Mainhagu, Jon; Morrison, C.; Truex, Michael J.; Oostrom, Martinus; Brusseau, Mark

    2014-10-20

    A method termed vapor-phase tomography has recently been proposed to characterize the distribution of volatile organic contaminant mass in vadose-zone source areas, and to measure associated three-dimensional distributions of local contaminant mass discharge. The method is based on measuring the spatial variability of vapor flux, and thus inherent to its effectiveness is the premise that the magnitudes and temporal variability of vapor concentrations measured at different monitoring points within the interrogated area will be a function of the geospatial positions of the points relative to the source location. A series of flow-cell experiments was conducted to evaluate this premise. A well-defined source zone was created by injection and extraction of a non-reactive gas (SF6). Spatial and temporal concentration distributions obtained from the tests were compared to simulations produced with a mathematical model describing advective and diffusive transport. Tests were conducted to characterize both areal and vertical components of the application. Decreases in concentration over time were observed for monitoring points located on the opposite side of the source zone from the local–extraction point, whereas increases were observed for monitoring points located between the local–extraction point and the source zone. The results illustrate that comparison of temporal concentration profiles obtained at various monitoring points gives a general indication of the source location with respect to the extraction and monitoring points.

  19. Rock Valley Source Physics Experiment Preparation: Earthquake Relocation and Attenuation Structure Characterization

    NASA Astrophysics Data System (ADS)

    Pyle, M. L.; Walter, W. R.; Myers, S.; Pasyanos, M. E.; Smith, K. D.

    2012-12-01

    The science of nuclear test monitoring relies on seismic methods to distinguish explosion from earthquakes sources. Unfortunately, the physics behind how an explosion generates seismic waves, particularly shear waves, remains incompletely understood. The Source Physics Experiments (SPE) are an ongoing series of chemical explosions designed to address this problem and advance explosion monitoring physics and associated simulation codes. The current series of explosions are located in the Climax Stock granite on the Nevada National Security Site (NNSS). A future candidate for the SPEs would allow us to make a direct comparison of earthquake and explosion sources by detonating an explosion at a well constrained earthquake hypocenter and recording the resulting signals from each source at common receivers. This possibility arises from an area of unusually shallow seismicity in the Rock Valley area of the southern NNSS. While most tectonic earthquakes occur at depths greater than 5 km, a sequence of unusually shallow earthquakes with depths of 1-2 km occurred in Rock Valley in May of 1993. The main shock had a magnitude of approximately 3.7 and 11 more events in the sequence had magnitudes over 2. The shallow depths of these events were well constrained by temporary stations deployed at the time by the University of Nevada-Reno (UNR). As part of a feasibility study for a future Rock Valley SPE, LLNL, UNR and NSTec are working to re-instrument and improve our understanding of the Rock Valley region. Rock Valley is a complex set of left oblique-slip segmented fault blocks; it is a regular source region for small magnitude shallow earthquakes. A dense seismic network was operated in the southern NNSS through the Yucca Mountain project (1992-2010). Although much of the older network has been removed, six new Rock Valley telemetered seismic stations located at both original 1993 sites and additional sites, have been installed and operating since early 2011. In order to

  20. Rural continental aerosol properties and processes observed during the Hohenpeissenberg Aerosol Characterization Experiment (HAZE2002)

    NASA Astrophysics Data System (ADS)

    Hock, N.; Schneider, J.; Borrmann, S.; Römpp, A.; Moortgat, G.; Franze, T.; Schauer, C.; Pöschl, U.; Plass-Dülmer, C.; Berresheim, H.

    2007-06-01

    Detailed investigations of the chemical and microphysical properties of rural continental aerosols were performed during the HAZE2002 experiment, which was conducted in May 2002 at the Meteorological Observatory Hohenpeissenberg (DWD) in Southern Germany. The online measurement data and techniques included: size-resolved chemical composition of submicron particles by aerosol mass spectrometry (AMS); total particle number concentrations and size distributions over the diameter range of 3 nm to 9 μm (CPC, SMPS, OPC); monoterpenes determined by gas chromatography- ion trap mass spectrometry; OH and H2SO4 determined by atmospheric pressure chemical ionization mass spectrometry (CIMS). Filter sampling and offline analytical techniques were used to determine: fine particle mass (PM2.5), organic, elemental and total carbon in PM2.5 (OC2.5, EC2.5, TC2.5), and selected organic compounds (dicarboxylic acids, polycyclic aromatic hydrocarbons, proteins). Overall, the non-refractory components of submicron particles detected by aerosol mass spectrometry (PM1, 6.6±5.4 μg m-3, arithmetic mean and standard deviation) accounted for ~62% of PM2.5 determined by filter gravimetry (10.6±4.7 μg m-3). The relative proportions of non-refractory submicron particle components were: 11% ammonium, 19% nitrate, 20% sulfate, and 50% organics (OM1). In spite of strongly changing meteorological conditions and absolute concentration levels of particulate matter (3-13 μg m-3 PM1), OM1 was closely correlated with PM1 (r2=0.9) indicating a near-constant ratio of non-refractory organics and inorganics. In contrast, the ratio of nitrate to sulfate was highly dependent on temperature (14-32°C) and relative humidity (20-100%), which could be explained by thermodynamic model calculations of NH3/HNO3/NH4NO3 gas-particle partitioning. From the combination of optical and other sizing techniques (OPC, AMS, SMPS), an average refractive index of 1.40-1.45 was inferred for the measured rural aerosol

  1. Ground-based aerosol characterization during the South American Biomass Burning Analysis (SAMBBA) field experiment

    NASA Astrophysics Data System (ADS)

    Brito, J.; Rizzo, L. V.; Morgan, W. T.; Coe, H.; Johnson, B.; Haywood, J.; Longo, K.; Freitas, S.; Andreae, M. O.; Artaxo, P.

    2014-11-01

    This paper investigates the physical and chemical characteristics of aerosols at ground level at a site heavily impacted by biomass burning. The site is located near Porto Velho, Rondônia, in the southwestern part of the Brazilian Amazon rainforest, and was selected for the deployment of a large suite of instruments, among them an Aerosol Chemical Speciation Monitor. Our measurements were made during the South American Biomass Burning Analysis (SAMBBA) field experiment, which consisted of a combination of aircraft and ground-based measurements over Brazil, aimed to investigate the impacts of biomass burning emissions on climate, air quality, and numerical weather prediction over South America. The campaign took place during the dry season and the transition to the wet season in September/October 2012. During most of the campaign, the site was impacted by regional biomass burning pollution (average CO mixing ratio of 0.6 ppm), occasionally superimposed by intense (up to 2 ppm of CO), freshly emitted biomass burning plumes. Aerosol number concentrations ranged from ~1000 cm-3 to peaks of up to 35 000 cm-3 (during biomass burning (BB) events, corresponding to an average submicron mass mean concentrations of 13.7 μg m-3 and peak concentrations close to 100 μg m-3. Organic aerosol strongly dominated the submicron non-refractory composition, with an average concentration of 11.4 μg m-3. The inorganic species, NH4, SO4, NO3, and Cl, were observed, on average, at concentrations of 0.44, 0.34, 0.19, and 0.01 μg m-3, respectively. Equivalent black carbon (BCe) ranged from 0.2 to 5.5 μg m-3, with an average concentration of 1.3 μg m-3. During BB peaks, organics accounted for over 90% of total mass (submicron non-refractory plus BCe), among the highest values described in the literature. We examined the ageing of biomass burning organic aerosol (BBOA) using the changes in the H : C and O : C ratios, and found that throughout most of the aerosol processing (O : C &cong

  2. Ground based aerosol characterization during the South American Biomass Burning Analysis (SAMBBA) field experiment

    NASA Astrophysics Data System (ADS)

    Brito, J.; Rizzo, L. V.; Morgan, W. T.; Coe, H.; Johnson, B.; Haywood, J.; Longo, K.; Freitas, S.; Andreae, M. O.; Artaxo, P.

    2014-05-01

    This paper investigates the physical and chemical characteristics of aerosols at ground level at a site heavily impacted by biomass burning. The site is located near Porto Velho, Rondônia, in the Southwestern part of the Brazilian Amazon forest, and was selected for the deployment of a large suite of instruments, among them an Aerosol Chemical Speciation Monitor. Our measurements were made during the South American Biomass Burning Analysis (SAMBBA) field experiment, which consisted of a combination of aircraft and ground based measurements over Brazil, aiming to investigate the impacts of biomass burning emissions on climate, air quality, and numerical weather prediction over South America. The campaign took place during the dry season and the transition to the wet season in September/October 2012. During most of the campaign, the site was impacted by regional biomass burning pollution (average CO mixing ratio of 0.6 ppm), occasionally superimposed by intense (up to 2 ppm of CO), freshly emitted biomass burning plumes. Aerosol number concentrations ranged from ∼1000 cm-3 to peaks of up to 35 000 cm-3 during biomass burning (BB) events, corresponding to an average submicron mass mean concentrations of 13.7 μg m-3 and peak concentrations close to 100 μg m-3. Organic aerosol strongly dominated the submicron non-refractory composition, with an average concentration of 11.4 μg m-3. The inorganic species, NH4, SO4, NO3, and Cl, were observed on average at concentrations of 0.44, 0.34, 0.19, and 0.01 μg m-3, respectively. Equivalent Black Carbon (BCe) ranged from 0.2 to 5.5 μg m-3, with an average concentration of 1.3 μg m-3. During BB peaks, organics accounted for over 90% of total mass (submicron non-refractory plus BCe), among the highest values described in the literature. We examined the ageing of Biomass Burning Organic Aerosol (BBOA) using the changes in the H : C and O : C ratios, and found that throughout most of the aerosol processing (O : C ≅ 0

  3. Isolation and Characterization of a Novel Bat Coronavirus Closely Related to the Direct Progenitor of Severe Acute Respiratory Syndrome Coronavirus.

    PubMed

    Yang, Xing-Lou; Hu, Ben; Wang, Bo; Wang, Mei-Niang; Zhang, Qian; Zhang, Wei; Wu, Li-Jun; Ge, Xing-Yi; Zhang, Yun-Zhi; Daszak, Peter; Wang, Lin-Fa; Shi, Zheng-Li

    2016-03-01

    We report the isolation and characterization of a novel bat coronavirus which is much closer to the severe acute respiratory syndrome coronavirus (SARS-CoV) in genomic sequence than others previously reported, particularly in its S gene. Cell entry and susceptibility studies indicated that this virus can use ACE2 as a receptor and infect animal and human cell lines. Our results provide further evidence of the bat origin of the SARS-CoV and highlight the likelihood of future bat coronavirus emergence in humans. PMID:26719272

  4. Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

    PubMed Central

    Buyel, Johannes Felix; Fischer, Rainer

    2014-01-01

    Plants provide multiple benefits for the production of biopharmaceuticals including low costs, scalability, and safety. Transient expression offers the additional advantage of short development and production times, but expression levels can vary significantly between batches thus giving rise to regulatory concerns in the context of good manufacturing practice. We used a design of experiments (DoE) approach to determine the impact of major factors such as regulatory elements in the expression construct, plant growth and development parameters, and the incubation conditions during expression, on the variability of expression between batches. We tested plants expressing a model anti-HIV monoclonal antibody (2G12) and a fluorescent marker protein (DsRed). We discuss the rationale for selecting certain properties of the model and identify its potential limitations. The general approach can easily be transferred to other problems because the principles of the model are broadly applicable: knowledge-based parameter selection, complexity reduction by splitting the initial problem into smaller modules, software-guided setup of optimal experiment combinations and step-wise design augmentation. Therefore, the methodology is not only useful for characterizing protein expression in plants but also for the investigation of other complex systems lacking a mechanistic description. The predictive equations describing the interconnectivity between parameters can be used to establish mechanistic models for other complex systems. PMID:24514765

  5. Chem-prep PZT 95/5 for neutron generator applicatios : powder preparation characterization utilizing design of experiments.

    SciTech Connect

    Lockwood, Steven John; Rodman-Gonzales, Emily Diane; Voigt, James A.; Moore, Diana Lynn

    2003-07-01

    Niobium doped PZT 95/5 (lead zirconate-lead titanate) is the material used in voltage bars for all ferroelectric neutron generator power supplies. In June of 1999, the transfer and scale-up of the Sandia Process from Department 1846 to Department 14192 was initiated. The laboratory-scale process of 1.6 kg has been successfully scaled to a production batch quantity of 10 kg. This report documents efforts to characterize and optimize the production-scale process utilizing Design of Experiments methodology. Of the 34 factors identified in the powder preparation sub-process, 11 were initially selected for the screening design. Additional experiments and safety analysis subsequently reduced the screening design to six factors. Three of the six factors (Milling Time, Media Size, and Pyrolysis Air Flow) were identified as statistically significant for one or more responses and were further investigated through a full factorial interaction design. Analysis of the interaction design resulted in developing models for Powder Bulk Density, Powder Tap Density, and +20 Mesh Fraction. Subsequent batches validated the models. The initial baseline powder preparation conditions were modified, resulting in improved powder yield by significantly reducing the +20 mesh waste fraction. Response variation analysis indicated additional investigation of the powder preparation sub-process steps was necessary to identify and reduce the sources of variation to further optimize the process.

  6. Comparison of Aerosol Single Scattering Albedos Derived By Diverse Techniques in Two North Atlantic Experiments

    NASA Technical Reports Server (NTRS)

    Russell, P. B.; Redemann, J.; Schmid, B.; Bergstrom, R. W.; Livingston, J. M.; McIntosh, D. M.; Hartley, S.; Hobbs, P. V.; Quinn, P. K.; Carrico, C. M.; Hipskind, R. Stephen (Technical Monitor)

    2000-01-01

    Aerosol single scattering albedo w (the ratio of scattering to extinction) is important in determining aerosol climatic effects, in explaining relationships between calculated and measured radiative fluxes, and in retrieving aerosol optical depths from satellite radiances. Recently, two experiments in the North Atlantic region, TARFOX and ACE-2, determined aerosol w by a variety of techniques. The techniques included fitting of calculated to measured fluxes; retrievals of w from skylight radiances; best fits of complex refractive index to profiles of backscatter, extinction, and size distribution; and in situ measurements of scattering and absorption at the surface and aloft. Both TARFOX and ACE-2 found a fairly wide range of values for w at midvisible wavelengths, with 0.85 less than wmidvis less than 0.99 for the marine aerosol impacted by continental pollution. Frequency distributions of w could usually be approximated by lognormals in wmax-w, with some occurrence of bimodality, suggesting the influence of different aerosol sources or processing. In both TARFOX and ACE-2, closure tests between measured and calculated radiative fluxes yielded best-fit values of wmidvis of 0.90+/-0.04 for the polluted boundary layer. Although these results have the virtue of describing the column aerosol unperturbed by sampling, they are subject to questions about representativeness and possible artifacts (e.g., unknown gas absorption). The other techniques gave larger values for wmidvis for the polluted boundary layer, with a typical result of wmidvis = 0.95+/-0.04, Current uncertainties in vv are large in terms of climate effects. More tests are needed of the consistency among different methods and of humidification effects on w.

  7. Concept model of the formation process of humic acid-kaolin complexes deduced by trichloroethylene sorption experiments and various characterizations.

    PubMed

    Zhu, Xiaojing; He, Jiangtao; Su, Sihui; Zhang, Xiaoliang; Wang, Fei

    2016-05-01

    To explore the interactions between soil organic matter and minerals, humic acid (HA, as organic matter), kaolin (as a mineral component) and Ca(2+) (as metal ions) were used to prepare HA-kaolin and Ca-HA-kaolin complexes. These complexes were used in trichloroethylene (TCE) sorption experiments and various characterizations. Interactions between HA and kaolin during the formation of their complexes were confirmed by the obvious differences between the Qe (experimental sorbed TCE) and Qe_p (predicted sorbed TCE) values of all detected samples. The partition coefficient kd obtained for the different samples indicated that both the organic content (fom) and Ca(2+) could significantly impact the interactions. Based on experimental results and various characterizations, a concept model was developed. In the absence of Ca(2+), HA molecules first patched onto charged sites of kaolin surfaces, filling the pores. Subsequently, as the HA content increased and the first HA layer reached saturation, an outer layer of HA began to form, compressing the inner HA layer. As HA loading continued, the second layer reached saturation, such that an outer-third layer began to form, compressing the inner layers. In the presence of Ca(2+), which not only can promote kaolin self-aggregation but can also boost HA attachment to kaolin, HA molecules were first surrounded by kaolin. Subsequently, first and second layers formed (with inner layer compression) via the same process as described above in the absence of Ca(2+), except that the second layer continued to load rather than reach saturation, within the investigated conditions, because of enhanced HA aggregation caused by Ca(2+). PMID:26933902

  8. One Year of Doppler Lidar Observations Characterizing Boundary Layer Wind, Turbulence, and Aerosol Structure During the Indianapolis Flux Experiment

    NASA Astrophysics Data System (ADS)

    Hardesty, R. M.; Brewer, A.; Shepson, P. B.; Cambaliza, M. O. L.; Salmon, O. E.; Heimburger, A. M. F.; Davis, K. J.; Lauvaux, T.; McGowan, L. E.; Miles, N. L.; Richardson, S.; Sarmiento, D. P.; Karion, A.; Sweeney, C.; Iraci, L. T.; Hillyard, P. W.; Podolske, J. R.; Gurney, K. R.; Razlivanov, I. N.; Song, Y.; Turnbull, J. C.; Whetstone, J. R.; Possolo, A.; Prasad, K.

    2014-12-01

    The Indianapolis Flux Experiment (INFLUX) is aimed at improving methods for estimation of greenhouse gas emissions at urban scales. INFLUX observational components include several-times-per-month aircraft measurements of gas concentrations and meteorological parameters, as well as a number of towers observing CO2, CH4, and CO and a single continuously operating Doppler lidar to estimate wind, turbulence and aerosol structure in the boundary layer. The observations are used to develop top-down emissions estimates from the aircraft measurements and as input to inversion models. The Doppler lidar provides information on boundary layer structure for both the aircraft and inversion studies. A commercial Doppler lidar characterized by low pulse energy and high pulse repetition rate has operated for well over a year at a site NE of downtown Indianapolis. The lidar produces profiles of horizontal wind speed, vertical velocity variance, and aerosol structure two to three times per hour. These data are then used to investigate boundary layer mixing and thickness and horizontal transport as inputs for the flux calculations. During its one year deployment the lidar generally operated reliably with few outages. Comparisons with aircraft spirals over the site and with the NOAA High Resolution research Doppler lidar deployed to Indianapolis for one month during May, 2014, were used to assess the performance of the INFLUX lidar. Measurements agreed quite well when aerosol loading was sufficient for lidar observations throughout the boundary layer. However, low aerosol loading during some periods limited the range of the lidar and precluded characterization of the full boundary layer. We present an overall assessment of the commercial Doppler lidar for providing the needed information on boundary layer structure for emission estimations, and show variability of the boundary layer observations over diurnal, seasonal, and annual cycles. Recommendations on system design changes to

  9. Reactive Flow Experiments to Characterize Porosity and Permeability Evolution during CO2 Transport in Weyburn-Midale Carbonate Rocks

    NASA Astrophysics Data System (ADS)

    Smith, M. M.; Sholokhova, Y.; Hao, Y.; Carroll, S.

    2011-12-01

    We investigated the relative effects of CO2-induced disequilibrium and pre-existing mineralogy and void space heterogeneity on permeability development in carbonate core samples from the Weyburn-Midale hydrocarbon reservoir (Canada). The aim of our work was to use detailed pre- and post-experimental x-ray computed tomography (XCMT) imaging, as well as geochemical data, to constrain reactive transport models that predict the evolution of pore space and permeability for geologic storage of CO2 in enhanced oil recovery (EOR) fields. A total of nine core-flooding experiments were completed, using three distinct rock types (tight limestone, porous dolostone, and evaporite caprock) to represent the range of natural reservoir physical and chemical heterogeneity. Experiments were conducted under 25 MPa confining pressure, 60C temperature, and elevated salinity conditions, with pCO2 levels from 0.5-3 MPa. The coupling of intensive characterization with pressure/permeability and solution chemistry measurements provided powerful tools for interpreting and correlating mineral reactions and stability with pre-existing features and heterogeneities. We observed increased carbonate mass transfer rates, stable dissolution fronts, and greater volumes of dissolved minerals for cores with relatively homogeneous pore networks. Samples with more heterogeneous pore size distributions responded with variable mass transfer rates and development of fast transport pathways in regions with preexisting fractures. We infer that the breakthrough of these preferential fluid pathways leads to reductions in available reactive surface area, allowing undersaturated fluids to be transported through the cores despite relatively fast carbonate dissolution kinetics.

  10. Satellite Characterization of Biomass Burning: Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope Study of Combustion Experiments

    NASA Astrophysics Data System (ADS)

    Padilla, D.; Steiner, J. C.

    2005-12-01

    Fourier Transform Infrared (FTIR) examination of the combustion products of selected forest materials using a meeker burner flame at temperatures up to 500 degrees Celsius produces a cluster of broad distinct peaks throughout the 400 to 4000 cm-1 wavenumber interval. Distinct bands bracketed by wavenumbers 400-700, 1500-1700, 2200-2400 and 3300-3600 cm-1 show variable intensity with an average difference between the least absorbing and most strongly absorbing species of approximately fifty percent. Given that spectral band differences of ten percent are within the range of modern satellite spectrometers, these band differences are of potential value for discriminating between fires that are impacting a range of vegetation types. Corresponding scanning electron microscope and energy dispersive micro-chemical (SEM/ED) analysis establishes that the evolved soot particles exhibit a characteristic rounded morphology, are carbon rich and host a wide range of adsorbed elements, including calcium, aluminum, potassium, silicon, sulfur and trace nitrogen. Combustion experiments involving leaves and branches as a subset of the biomass experiments at 200-500 degrees Celsius yield a similar broad background, but with peak shifts for maxima residing at less than 1700 cm-1. Additional peaks appear in the ranges 1438-1444, 875 and 713 cm-1. These peak are of potential use for discriminating between hot and smoldering fires, and between soot and smoke yields from green woods and whole-wood or lumber. The spectral shifts noted for low temperature smoldering conditions are in the vicinity of those cited for green vegetation and may not be resolved by present satellite platforms. Nevertheless, the experimental peak data set is of potential use for discriminating between a conflagration or accentuated fire and one characterized by smoldering at low temperature. SEM/ED analysis of the combusted leaf, branch, bark and various crown assemblages yields comparable morphological and

  11. Characterization of deuterium clusters mixed with helium gas for an application in beam-target-fusion experiments

    DOE PAGESBeta

    Bang, W.; Quevedo, H. J.; Bernstein, A. C.; Dyer, G.; Ihn, Y. S.; Cortez, J.; Aymond, F.; Gaul, E.; Donovan, M. E.; Barbui, M.; et al

    2014-12-10

    We measured the average deuterium cluster size within a mixture of deuterium clusters and helium gas by detecting Rayleigh scattering signals. The average cluster size from the gas mixture was comparable to that from a pure deuterium gas when the total backing pressure and temperature of the gas mixture were the same as those of the pure deuterium gas. According to these measurements, the average size of deuterium clusters depends on the total pressure and not the partial pressure of deuterium in the gas mixture. To characterize the cluster source size further, a Faraday cup was used to measure themore » average kinetic energy of the ions resulting from Coulomb explosion of deuterium clusters upon irradiation by an intense ultrashort pulse. The deuterium ions indeed acquired a similar amount of energy from the mixture target, corroborating our measurements of the average cluster size. As the addition of helium atoms did not reduce the resulting ion kinetic energies, the reported results confirm the utility of using a known cluster source for beam-target-fusion experiments by introducing a secondary target gas.« less

  12. Characterization of deuterium clusters mixed with helium gas for an application in beam-target-fusion experiments

    SciTech Connect

    Bang, W.; Quevedo, H. J.; Bernstein, A. C.; Dyer, G.; Ihn, Y. S.; Cortez, J.; Aymond, F.; Gaul, E.; Donovan, M. E.; Barbui, M.; Bonasera, A.; Natowitz, J. B.; Albright, B. J.; Fernández, J. C.; Ditmire, T.

    2014-12-10

    We measured the average deuterium cluster size within a mixture of deuterium clusters and helium gas by detecting Rayleigh scattering signals. The average cluster size from the gas mixture was comparable to that from a pure deuterium gas when the total backing pressure and temperature of the gas mixture were the same as those of the pure deuterium gas. According to these measurements, the average size of deuterium clusters depends on the total pressure and not the partial pressure of deuterium in the gas mixture. To characterize the cluster source size further, a Faraday cup was used to measure the average kinetic energy of the ions resulting from Coulomb explosion of deuterium clusters upon irradiation by an intense ultrashort pulse. The deuterium ions indeed acquired a similar amount of energy from the mixture target, corroborating our measurements of the average cluster size. As the addition of helium atoms did not reduce the resulting ion kinetic energies, the reported results confirm the utility of using a known cluster source for beam-target-fusion experiments by introducing a secondary target gas.

  13. High Efficiency Particulate Air (HEPA) Filter Generation, Characterization, and Disposal Experiences at the Oak Ridge National Laboratory

    SciTech Connect

    Coffey, D. E.

    2002-02-28

    High Efficiency Particulate Air filtration is an essential component of the containment and ventilation systems supporting the research and development activities at the Oak Ridge National Laboratory. High Efficiency Particulate Air filters range in size from 7.6cm (3 inch) by 10.2 cm (4 inch) cylindrical shape filters to filter array assemblies up to 2.1 m (7 feet) high by 1.5 m (5 feet) wide. Spent filters are grouped by contaminates trapped in the filter media and become one of the components in the respective waste stream. Waste minimization and pollution prevention efforts are applied for both radiological and non-radiological applications. Radiological applications include laboratory hoods, glove boxes, and hot cells. High Efficiency Particulate Air filters also are generated from intake or pre-filtering applications, decontamination activities, and asbestos abatement applications. The disposal avenues include sanitary/industrial waste, Resource Conservation and Recovery Act and Toxic Substance Control Act, regulated waste, solid low-level waste, contact handled transuranic, and remote handled transuranic waste. This paper discusses characterization and operational experiences associated with the disposal of the spent filters across multiple applications.

  14. Hydrothermal Synthesis and Characterization of a Metal-Organic Framework by Thermogravimetric Analysis, Powder X-Ray Diffraction, and Infrared Spectroscopy: An Integrative Inorganic Chemistry Experiment

    ERIC Educational Resources Information Center

    Crane, Johanna L.; Anderson, Kelly E.; Conway, Samantha G.

    2015-01-01

    This advanced undergraduate laboratory experiment involves the synthesis and characterization of a metal-organic framework with microporous channels that are held intact via hydrogen bonding of the coordinated water molecules. The hydrothermal synthesis of Co[subscript 3](BTC)[subscript 2]·12H[subscript 2]O (BTC = 1,3,5-benzene tricarboxylic acid)…

  15. Detailed characterization of a Comparative Reactivity Method (CRM) instrument for ambient OH reactivity measurements: experiments vs. modeling

    NASA Astrophysics Data System (ADS)

    Michoud, Vincent; Locoge, Nadine; Dusanter, Sébastien

    2015-04-01

    The Hydroxyl radical (OH) is the main daytime oxidant in the troposphere, leading to the oxidation of Volatile Organic Compounds (VOCs) and the formation of harmful pollutants such as ozone (O3) and Secondary Organic Aerosols (SOA). While OH plays a key role in tropospheric chemistry, recent studies have highlighted that there are still uncertainties associated with the OH budget, i.e the identification of sources and sinks and the quantification of production and loss rates of this radical. It has been demonstrated that ambient measurements of the total OH loss rate (also called total OH reactivity) can be used to identify and reduce these uncertainties. In this context, the Comparative Reactivity Method (CRM), developed by Sinha et al. (ACP, 2008), is a promising technique to measure total OH reactivity in ambient air and has already been used during several field campaigns. This technique relies on monitoring competitive reactions of OH with ambient trace gases and a reference compound (pyrrole) in a sampling reactor to derive ambient OH reactivity. However, this technique requires a complex data processing chain that has yet to be carefully investigated in the laboratory. In this study, we present a detailed characterization of a CRM instrument developed at Mines Douai, France. Experiments have been performed to investigate the dependence of the CRM response on humidity, ambient NOx levels, and the pyrrole-to-OH ratio inside the sampling reactor. Box modelling of the chemistry occurring in the reactor has also been performed to assess our theoretical understanding of the CRM measurement. This work shows that the CRM response is sensitive to both humidity and NOx, which can be accounted for during data processing using parameterizations depending on the pyrrole-to-OH ratio. The agreement observed between laboratory studies and model results suggests a good understanding of the chemistry occurring in the sampling reactor and gives confidence in the CRM

  16. Manipulation and characterization of thin-film interfacial chemistry: Sol-gel deposition and single molecule tracking experiments

    NASA Astrophysics Data System (ADS)

    Barhoum, Moussa

    Single molecule trajectories of 1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbo - cyanine perchlorate (DiI) fluorophores diffusing on planar supported 1,2-dimyristoyl-snglycero- 3-phosphocholine (DMPC) lipid bilayers imaged through total internal reflection fluorescence (TIRF) microscopy at different temperatures are investigated. The spatial resolution limit for detecting molecular motion is evaluated by characterizing the apparent motion which arises from the limited signal-to-noise ratio (S/N) of imaged and simulated stationary DiI molecules. Statistical criteria for reliably distinguishing molecular motion from stationary molecules using F-test statistics, including the computation of local signal-to-noise ratios are then established and used for reliably detecting subdiffraction motion of DiI molecules on DMPC. The same single molecule tracking concept is used in investigating the temperature dependence of subdiffraction diffusional confinement of single Rhodamine 6G molecules in polymer brushes of poly (N-isopropylacrylamide), pNIPAAm, above and below its lower critical solution temperature (LCST) of 32°C. Reliably distinguishing subdiffraction molecular motion from stationary events is crucial in validating the application of single molecule tracking experiment in probing nanometersized hydrophobic environments of polymer structure. A versatile and rapid sol-gel technique for the fabrication of high quality one-dimensional photonic bandgap materials was developed. Silica/titania multilayer materials are fabricated by a sol-gel chemistry route combined with dip-coating onto planar or curved substrate. A shock-cooling step immediately following the thin film heat-treatment process is introduced. The versatility of this sol-gel method is demonstrated by the fabrication of various Bragg stack-type materials with fine-tuned optical properties. Measured optical properties show good agreement with theoretical simulations confirming the high quality of these sol

  17. Detailed characterization and preliminary adsorption model for materials for an intermediate-scale reactive-transport experiment

    SciTech Connect

    Ward, D.B.; Bryan, C.R.; Siegel, M.D.

    1994-12-31

    An experiment involving migration of fluid and tracers (Li, Br, Ni) through a 6-m-high x 3-m-dia caisson Wedron 510 sand, is being carried out for Yucca Mountain Site Characterization Project. Sand`s surface chemistry of the sand was studied and a preliminary surface-complexation model of Ni adsorption formulated for transport calculations. XPS and leaching suggest that surface of the quartz sand is partially covered by thin layers of Fe-oxyhydroxide and Ca-Mg carbonate and by flakes of kaolinite. Ni adsorption by the sand is strongly pH-dependent, showing no adsorption at pH 5 and near-total adsorption at pH 7. Location of adsorption edge is independent of ionic strength and dissolved Ni concentration; it is shifted to slightly lower pH with higher pCO2 and to slightly higher pH by competition with Li. Diminished adsorption at alkiline pH with higher pCO2 implies formation of dissolved Ni-carbonato complexes. Ni adsorption edges for goethite and quartz, two components of the sand were also measured. Ni adsorption on pure quartz is only moderately pH-dependent and differs in shape and location from that of the sand, whereas Ni adsorption by goethite is strongly pH-dependent. A triple-layer surface-complexation model developed for goethite provides a good fit to the Ni-adsorption curve of the sand. Based on this model, the apparent surface area of the Fe-oxyhydroxide coating is estimated to be 560 m{sup 2}/g, compatible with its occurrence as amorphous Fe-oxyhydroxide. Potentiometric titrations on sand also differ from pure quartz and suggest that effective surface area of sand may be much greater than that measured by N{sub 2}-BET gas adsorption. Attempts to model the adsorption of bulk sand in terms of properties of pure end member components suggest that much of the sand surface is inert. Although the exact Ni adsorption mechanisms remain ambiguous, this preliminary adsorption model provides an initial set of parameters that can be used in transport calculations.

  18. The identification, characterization and optimization of small molecule probes of cysteine proteases: experiences of the Penn Center for Molecular Discovery with cathepsin B and cathepsin L.

    PubMed

    Huryn, Donna M; Smith, Amos B

    2009-01-01

    During the pilot phase of the NIH Molecular Library Screening Network, the Penn Center for Molecular Discovery focused on a series of projects aimed at high throughput screening and the development of probes of a variety of protease targets. This review provides our medicinal chemistry experience with two such targets--cathepsin B and cathepsin L. We describe our approach for hit validation, characterization and triage that led to a critical understanding of the nature of hits from the cathepsin B project. In addition, we detail our experience at hit identification and optimization that led to the development of a novel thiocarbazate probe of cathepsin L. PMID:19807666

  19. Hydrogeophysical characterization of transport processes in fractured rock by combining push-pull and single-hole ground penetrating radar experiments

    NASA Astrophysics Data System (ADS)

    Shakas, Alexis; Linde, Niklas; Baron, Ludovic; Bochet, Olivier; Bour, Olivier; Le Borgne, Tanguy

    2016-02-01

    The in situ characterization of transport processes in fractured media is particularly challenging due to the considerable spatial uncertainty on tracer pathways and dominant controlling processes, such as dispersion, channeling, trapping, matrix diffusion, ambient and density driven flows. We attempted to reduce this uncertainty by coupling push-pull tracer experiments with single-hole ground penetrating radar (GPR) time-lapse imaging. The experiments involved different injection fractures, chaser volumes and resting times, and were performed at the fractured rock research site of Ploemeur in France (H+ network, hplus.ore.fr/en). For the GPR acquisitions, we used both fixed and moving antenna setups in a borehole that was isolated with a flexible liner. During the fixed-antenna experiment, time-varying GPR reflections allowed us to track the spatial and temporal dynamics of the tracer during the push-pull experiment. During the moving antenna experiments, we clearly imaged the dominant fractures in which tracer transport took place, fractures in which the tracer was trapped for longer time periods, and the spatial extent of the tracer distribution (up to 8 m) at different times. This demonstrated the existence of strongly channelized flow in the first few meters and radial flow at greater distances. By varying the resting time of a given experiment, we identified regions affected by density-driven and ambient flow. These experiments open up new perspectives for coupled hydrogeophysical inversion aimed at understanding transport phenomena in fractured rock formations.

  20. Design, fabrication and characterization of multi-guard-ring furnished p+n-n+ silicon strip detectors for future HEP experiments

    NASA Astrophysics Data System (ADS)

    Lalwani, Kavita; Jain, Geetika; Dalal, Ranjeet; Ranjan, Kirti; Bhardwaj, Ashutosh

    2016-07-01

    Si detectors, in various configurations (strips and pixels), have been playing a key role in High Energy Physics (HEP) experiments due to their excellent vertexing and high precision tracking information. In future HEP experiments like upgrade of the Compact Muon Solenoid experiment (CMS) at the Large Hadron Collider (LHC), CERN and the proposed International Linear Collider (ILC), the Si tracking detectors will be operated in a very harsh radiation environment, which leads to both surface and bulk damage in Si detectors which in turn changes their electrical properties, i.e. change in the full depletion voltage, increase in the leakage current and decrease in the charge collection efficiency. In order to achieve the long term durability of Si-detectors in future HEP experiments, it is required to operate these detectors at very high reverse biases, beyond the full depletion voltage, thus requiring higher detector breakdown voltage. Delhi University (DU) is involved in the design, fabrication and characterization of multi-guard-ring furnished ac-coupled, single sided, p+n-n+ Si strip detectors for future HEP experiments. The design has been optimized using a two-dimensional numerical device simulation program (TCAD-Silvaco). The Si strip detectors are fabricated with eight-layers mask process using the planar fabrication technology by Bharat Electronic Lab (BEL), India. Further an electrical characterization set-up is established at DU to ensure the quality performance of fabricated Si strip detectors and test structures. In this work measurement results on non irradiated Si Strip detectors and test structures with multi-guard-rings using Current Voltage (IV) and Capacitance Voltage (CV) characterization set-ups are discussed. The effect of various design parameters, for example guard-ring spacing, number of guard-rings and metal overhang on breakdown voltage of test structures have been studied.

  1. Synthesis and Characterization of Copper Complexes with a Tridentate Nitrogen-Donor Ligand: An Integrated Research Experiment for Undergraduate Students

    ERIC Educational Resources Information Center

    Bussey, Katherine A.; Cavalier, Annie R.; Connell, Jennifer R.; Mraz, Margaret E.; Holderread, Ashley S.; Oshin, Kayode D.; Pintauer, Tomislav

    2015-01-01

    An integrated laboratory experiment applying concepts and techniques developed in organic chemistry, inorganic chemistry, and instrumental analysis is presented for use by students interested in undergraduate research. The experiment incorporates some advanced laboratory practices such as multistep organic synthesis and purification, detailed…

  2. Characterization of High Explosives and Other Energetic Compounds by Computational Chemistry and Molecular Modeling: Experiments for Undergraduate Curriculum

    ERIC Educational Resources Information Center

    Bumpus, John A.; Lewis, Anne; Stotts, Corey; Cramer, Christopher J.

    2007-01-01

    Experiments suited for the undergraduate instructional laboratory in which the heats of formation of several aliphatic and aromatic compounds are calculated, are described. The experiments could be used to introduce students to commercially available computational chemistry and its thermodynamics, while assess and compare the energy content of…

  3. Insights into Teachers' Experiences Implementing Garden Based Learning: Characterizing the Relationship between the Teacher and the Curriculum

    ERIC Educational Resources Information Center

    Selmer, Sarah J.; Luna, Melissa J.; Rye, James A.

    2015-01-01

    Background/Context: This study seeks insights into teachers' experiences implementing Garden-Based Learning (GBL) in an elementary school. The breadth of studies supporting the use of GBL in K-8 schools in the United States alongside the paucity of studies specific to teachers' experiences implementing GBL highlights the importance of this work.…

  4. Expression, purification, and characterization of a carbohydrate-active enzyme: A research-inspired methods optimization experiment for the biochemistry laboratory.

    PubMed

    Willbur, Jaime F; Vail, Justin D; Mitchell, Lindsey N; Jakeman, David L; Timmons, Shannon C

    2016-01-01

    The development and implementation of research-inspired, discovery-based experiences into science laboratory curricula is a proven strategy for increasing student engagement and ownership of experiments. In the novel laboratory module described herein, students learn to express, purify, and characterize a carbohydrate-active enzyme using modern techniques and instrumentation commonly found in a research laboratory. Unlike in a traditional cookbook-style experiment, students generate their own hypotheses regarding expression conditions and quantify the amount of protein isolated using their selected variables. Over the course of three 3-hour laboratory periods, students learn to use sterile technique to express a protein using recombinant DNA in E. coli, purify the resulting enzyme via affinity chromatography and dialysis, analyze the success of their purification scheme via SDS-PAGE, assess the activity of the enzyme via an HPLC-based assay, and quantify the amount of protein isolated via a Bradford assay. Following the completion of this experiment, students were asked to evaluate their experience via an optional survey. All students strongly agreed that this laboratory module was more interesting to them than traditional experiments because of its lack of a pre-determined outcome and desired additional opportunities to participate in the experimental design process. This experiment serves as an example of how research-inspired, discovery-based experiences can benefit both the students and instructor; students learned important skills necessary for real-world biochemistry research and a more concrete understanding of the research process, while generating new knowledge to enhance the scholarly endeavors of the instructor. PMID:26710673

  5. Characterization of spatially resolved high resolution x-ray spectrometers for high energy density physics and light source experiments

    SciTech Connect

    Hill, K. W. Bitter, M.; Delgado-Aparacio, L.; Efthimion, P.; Pablant, N. A.; Lu, J.; Beiersdorfer, P.; Chen, H.; Magee, E.

    2014-11-15

    A high resolution 1D imaging x-ray spectrometer concept comprising a spherically bent crystal and a 2D pixelated detector is being optimized for diagnostics of small sources such as high energy density physics (HEDP) and synchrotron radiation or x-ray free electron laser experiments. This instrument is used on tokamak experiments for Doppler measurements of ion temperature and plasma flow velocity profiles. Laboratory measurements demonstrate a resolving power, E/ΔE of order 10 000 and spatial resolution better than 10 μm. Initial tests of the high resolution instrument on HEDP plasmas are being performed.

  6. Targeted and untargeted data-dependent experiments for characterization of polycarbonate food-contact plastics by ultra high performance chromatography coupled to quadrupole orbitrap tandem mass spectrometry.

    PubMed

    Bignardi, Chiara; Cavazza, Antonella; Corradini, Claudio; Salvadeo, Paola

    2014-11-01

    Materials that come in contact with foods are potential sources of chemical food contamination. Consequently, characterization of their composition is of paramount importance considering the possible occurrence of several unknown molecules such as non-intentionally added substance (NIAS), residual monomers, degradation products, plastic additives and organic colorants. Previous studies concerning the characterization in terms of composition are focalized in the recognition of additives. To the best author's knowledge there are no scientific data about the composition of a plastic material in terms of colorants. In this work, an analytical method employing capillary ultra high-performance liquid chromatography and electrospray ionization quadrupole orbitrap high-resolution mass spectrometry (UHPLC-ESI Q-orbitrap) was exploited for characterization of polycarbonate for food contact material. Data-dependent experiments for targeted and untargeted analysis were employed after a total dissolution of polycarbonate samples and extraction of its components. The presence of common additives such as antioxidants and UV absorbers was confirmed by targeted analysis, while, the untargeted approach combined with the high mass accuracy of orbitrap technology allowed to identify for the first time some polycarbonate degradation products and the organic dyes effectively used for the coloration of plastic objects intended to come in contact with food. The present study shows the high potential of this technique in the field of material characterization aimed at food safety evaluation. PMID:25465011

  7. Educational X-Ray Experiments and XRF Measurements with a Portable Setup Adapted for the Characterization of Cultural Heritage Objects

    ERIC Educational Resources Information Center

    Sianoudis, I.; Drakaki, E.; Hein, A.

    2010-01-01

    It is common to modify valuable, sophisticated equipment, originally acquired for other purposes, to adapt it for the needs of educational experiments, with great didactic effectiveness. The present project concerns a setup developed from components of a portable system for energy dispersive x-ray fluorescence spectroscopy (EDXRF). Two educational…

  8. Characterization of Gas Chromatographic Liquid Phases Using McReynolds Constants. An Experiment for Instrumental Analysis Laboratory.

    ERIC Educational Resources Information Center

    Erskine, Steven R.; And Others

    1986-01-01

    Describes a laboratory experiment that is designed to aid in the understanding of the fundamental process involved in gas chromatographic separations. Introduces the Kovats retention index system for use by chemistry students to establish criteria for the optimal selection of gas chromatographic stationary phases. (TW)

  9. Characterizing the Effects of Sonic IR Variables on Turbine Disk Inspection Using a Design of Experiments Approach

    NASA Astrophysics Data System (ADS)

    Mayton, Donna; Spencer, Floyd; Alvarez, Carlos

    2005-04-01

    Sonic IR has shown potential as a viable nondestructive inspection technique, but many questions remain as to how to optimize the technique for detectability and reliability. There are many adjustable variables that influence the performance of this technique and it is not known how, or if, these variables interact with each other. In this program, sponsored by the Air Force Research Laboratory, we are taking a Design of Experiments (DoE) approach to the issue. Small samples with known fatigue cracks were tested and reported on previously. In our current work, we placed fatigue cracks in the non-symmetrical anti-rotation features of scrapped F100-PW-220 1st-stage high pressure turbine disks and conducted controlled experiments measuring peak crack response. This paper will discuss the design of this test matrix for the turbine disks, the experimental setup, test results, and conclusions.

  10. Characterization of metoprolol biodegradation and its transformation products generated in activated sludge batch experiments and in full scale WWTPs.

    PubMed

    Rubirola, A; Llorca, M; Rodriguez-Mozaz, S; Casas, N; Rodriguez-Roda, I; Barceló, D; Buttiglieri, G

    2014-10-15

    Metoprolol (MTP) is a compound of concern, considered as an emerging contaminant due to its high consumption, pseudopersistence and potential ecotoxicity. Activated sludge batch experiments were performed to evaluate the biological transformation of MTP and the formation of transformation products under different treatment conditions. Total MTP removal was obtained in aerobic conditions, and the formation of MTP known metabolites (metoprolol acid (MTPA), α-hydroxymetoprolol (α-HMTP) and O-desmethylmetoprolol (O-DMTP)) and unknown transformation products (TPs) was investigated. The three known metabolites and two new TPs generated along the experiments were identified by liquid chromatography coupled to high resolution mass spectrometry. For the two new TPs plausible structures were proposed based on the tentative identification. MTPA had the major ratio formation for the TPs identified along the experiments (up to 40% of initial MTP concentration after 96 h treatment) and its persistence through biological treatment was proven. Ecotoxicity studies using Vibrio fischeri bioluminescent bacteria in an acute toxicity test showed that MTP and its known TPs are not toxic with the exception of o-DMTP. Finally, MTP and its TPs were monitored in a full scale membrane bioreactor and in a full scale conventional urban wastewater treatment plant (WWTP) and the results were compared with those obtained in batch experiments. α-HMTP was detected for the first time in a WWTP influent whereas MTPA was detected in influent and effluent WWTP samples at much higher levels (up to 100 folds higher) than MTP itself remarking its high persistence. PMID:24973729

  11. BICEP2/Keck Array. IV. Optical Characterization and Performance of the BICEP2 and Keck Array Experiments

    NASA Astrophysics Data System (ADS)

    Ade, P. A. R.; Aikin, R. W.; Barkats, D.; Benton, S. J.; Bischoff, C. A.; Bock, J. J.; Bradford, K. J.; Brevik, J. A.; Buder, I.; Bullock, E.; Dowell, C. D.; Duband, L.; Filippini, J. P.; Fliescher, S.; Golwala, S. R.; Halpern, M.; Hasselfield, M.; Hildebrandt, S. R.; Hilton, G. C.; Hui, H.; Irwin, K. D.; Kang, J. H.; Karkare, K. S.; Kaufman, J. P.; Keating, B. G.; Kefeli, S.; Kernasovskiy, S. A.; Kovac, J. M.; Kuo, C. L.; Leitch, E. M.; Lueker, M.; Megerian, K. G.; Netterfield, C. B.; Nguyen, H. T.; O’Brient, R.; Ogburn, R. W., IV; Orlando, A.; Pryke, C.; Richter, S.; Schwarz, R.; Sheehy, C. D.; Staniszewski, Z. K.; Sudiwala, R. V.; Teply, G. P.; Thompson, K.; Tolan, J. E.; Turner, A. D.; Vieregg, A. G.; Weber, A. C.; Wong, C. L.; Yoon, K. W.; bicep2, The; Keck Array Collaborations

    2015-06-01

    bicep2 and the Keck Array are polarization-sensitive microwave telescopes that observe the cosmic microwave background (CMB) from the South Pole at degree angular scales in search of a signature of inflation imprinted as B-mode polarization in the CMB. bicep2 was deployed in late 2009, observed for three years until the end of 2012 at 150 GHz with 512 antenna-coupled transition edge sensor bolometers, and has reported a detection of B-mode polarization on degree angular scales. The Keck Array was first deployed in late 2010 and will observe through 2016 with five receivers at several frequencies (95, 150, and 220 GHz). bicep2 and the Keck Array share a common optical design and employ the field-proven bicep1 strategy of using small-aperture, cold, on-axis refractive optics, providing excellent control of systematics while maintaining a large field of view. This design allows for full characterization of far-field optical performance using microwave sources on the ground. Here we describe the optical design of both instruments and report a full characterization of the optical performance and beams of bicep2 and the Keck Array at 150 GHz.

  12. Batch experiments characterizing the reduction of Cr(VI) using suboxic material from a mildly reducing sand and gravel aquifer

    USGS Publications Warehouse

    Anderson, L.D.; Kent, D.B.; Davis, J.A.

    1994-01-01

    Batch experiments were conducted with sand collected from a shallow sand and gravel aquifer to identify the principal chemical reactions influencing the reduction of Cr(VI), so that field-observed Cr(VI) reduction could be described. The reduction appeared to be heterogeneous and occurred primarily on Fe(II)-bearing minerals. At only 1 wt %, the fine fraction (<64 ??m diameter) of the sediments dominated the amount of aqueous Cr(VI) reduction because of its greater reactivity and surface area. Although reduction of Cr(VI) increased with decreasing pH, small variations in the abundance of fine fraction among the replicate samples obscured pH trends in the batch experiments. Consistent results could only be obtained by separating the fine material from the sand and running parallel experiments on each fraction. As pH decreased (6.4 to 4.5), Cr(VI) reduction increased from 30 to 50 nmol/m2 for the sand fraction (64-1000 ??m) and from 130 to 200 nmol/m2 for the fine fraction. The amount of Cr(VI) reduced in both the sand-sized and fine material increased from 35 to 80 and from 130 to 1000 nmol/m2, respectively, for a 10-fold increase in Cr(VI)initial. A consistent description of the rate data was achieved by assuming that intraparticle diffusion limited the observed rate of reduction.

  13. Preliminary numerical modeling for the G-Tunnel welded tuff mining experiment; Yucca Mountain site characterization project

    SciTech Connect

    Johnson, R.L.; Bauer, S.J.

    1991-09-01

    Yucca Mountain, located in Southern Nevada, is to be considered as a potential site for a nuclear waste repository. Located in Rainier Mesa on the Nevada Test Site, G-Tunnel has been the site of a series of experiments, part of whose purpose is to evaluate measurement techniques for rock mechanics before testing in the Exploratory Shaft. Rainier Mesa is composed of welded and nonwelded tuffs that have thermal and mechanical properties and stress states similar to those of tuffs expected to be encountered at Yucca Mountain. A series of finite element calculations were performed to aid in designing instrumentation for the experiments in G-Tunnel and later to correlate with measured data. In this report are presented the results of the preliminary finite element calculations performed in conjunction with experimental measurements of drift convergence, or closure, and rock mass relaxation zones made before, during, and after completing the welded tuff mining experiment in G-Tunnel. Tape extensometer measurements of drift convergences and measurements determined by multiple point borehole extensometers are compared with corresponding calculated values using linear elastic and jointed rock material models. 9 refs., 25 figs., 7 tabs.

  14. Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments

    PubMed Central

    Baryshev, Sergey V.; Erck, Robert A.; Moore, Jerry F.; Zinovev, Alexander V.; Tripa, C. Emil; Veryovkin, Igor V.

    2013-01-01

    In materials science and engineering it is often necessary to obtain quantitative measurements of surface topography with micrometer lateral resolution. From the measured surface, 3D topographic maps can be subsequently analyzed using a variety of software packages to extract the information that is needed. In this article we describe how white light interferometry, and optical profilometry (OP) in general, combined with generic surface analysis software, can be used for materials science and engineering tasks. In this article, a number of applications of white light interferometry for investigation of surface modifications in mass spectrometry, and wear phenomena in tribology and lubrication are demonstrated. We characterize the products of the interaction of semiconductors and metals with energetic ions (sputtering), and laser irradiation (ablation), as well as ex situ measurements of wear of tribological test specimens. Specifically, we will discuss: Aspects of traditional ion sputtering-based mass spectrometry such as sputtering rates/yields measurements on Si and Cu and subsequent time-to-depth conversion. Results of quantitative characterization of the interaction of femtosecond laser irradiation with a semiconductor surface. These results are important for applications such as ablation mass spectrometry, where the quantities of evaporated material can be studied and controlled via pulse duration and energy per pulse. Thus, by determining the crater geometry one can define depth and lateral resolution versus experimental setup conditions. Measurements of surface roughness parameters in two dimensions, and quantitative measurements of the surface wear that occur as a result of friction and wear tests. Some inherent drawbacks, possible artifacts, and uncertainty assessments of the white light interferometry approach will be discussed and explained. PMID:23486006

  15. Characterizations of four specimens processed as a part of the M553 sphere forming experiment performed during the Skylab 1 and 2 flight

    NASA Technical Reports Server (NTRS)

    Hubbard, J. L.; Johnson, J. W.; Brown, J. L.

    1973-01-01

    Four specimens identified as SL-1.3, SL-1.8, SL-1.9 and SL-2.5 were submitted for metallurgical characterization. These specimens had been processed in the M512 Facility as a part of the M553 Sphere Forming Experiment performed during the Skylab 1/2 flight. Three of these specimens, SL-1.3, SL-1.8, and SL-2.5 were designed to be melted completely by the electron beam and detach themselves from their support posts and resolidify while floating free in the near zero gravity and vacuum environment of space. Specimens SL-1.3 and SL-1.8 were completely melted, but it is believed they did not leave their posts before solidifying. Specimen SL-2.5 was only partially melted. Specimen SL-1.9 was to be completely melted and retained on a large sting which was accomplished as planned. The nominal composition of the four specimens was: (1) SL-1.3 - Ni 12% Sn; (2) SL-1.8 - Ni 30% Cu; (3) SL-1.9 - Pure Ni; and (4) SL-2.5 - Pure Ni. These four specimens have been examined according to the Phase B Characterization Plan. The results are discussed and compared with similar characterization analyses run on ground base specimens.

  16. Educational x-ray experiments and XRF measurements with a portable setup adapted for the characterization of cultural heritage objects

    NASA Astrophysics Data System (ADS)

    Sianoudis, I.; Drakaki, E.; Hein, A.

    2010-05-01

    It is common to modify valuable, sophisticated equipment, originally acquired for other purposes, to adapt it for the needs of educational experiments, with great didactic effectiveness. The present project concerns a setup developed from components of a portable system for energy dispersive x-ray fluorescence spectroscopy (EDXRF). Two educational modules have been developed on the basis of this setup. Module 1 comprises a series of x-ray laboratory exercises investigating basic principles, such as the verification of Moseley's law, Compton's law and the Lambert-Beer law. Module 2 concerns the calibration of the XRF with reference materials, aiming to get quantitative measurements of the elemental composition of objects of cultural interest. The application of the calibrated experimental setup is demonstrated with indicative measurements of metal objects and pigments of wall paintings, in order to discuss their spectra, and their qualitative and quantitative analyses. The setup and the applied experiments are designed as an educational package of laboratory exercises on the one hand for students in natural sciences, and on the other for the education of students who will work in the field of cultural heritage, such as conservation science or archaeological science.

  17. Characterization of Fast Ion Absorption of the High Harmonic Fast Wave in the National Spherical Torus Experiment

    SciTech Connect

    A.L. Rosenberg; J.E. Menard; J.R. Wilson; S. Medle; C.K. Phillips; R. Andre; D.S. Darro; R.J. Dumont; B.P. LeBlanc; M.H. Redi; T.K. Mau; E. F. Jaeger; P.M. Ryan; D.W. Swain; R.W. Harvey; J. Egedal; the NSTX Team

    2003-08-19

    Ion absorption of the high harmonic fast wave in a spherical torus is of critical importance to assessing the viability of the wave as a means of heating and driving current. Analysis of recent National Spherical Torus Experiment (NSTX) shots has revealed that under some conditions when neutral beam and radio-frequency (RF) power are injected into the plasma simultaneously, a fast ion population with energy above the beam injection energy is sustained by the wave. In agreement with modeling, these experiments find the RF-induced fast ion tail strength and neutron rate at lower B-fields to be less enhanced, likely due to a larger beta profile, which promotes greater off-axis absorption where the fast ion population is small. Ion loss codes find the increased loss fraction with decreased B insufficient to account for the changes in tail strength, providing further evidence that this is an RF interaction effect. Though greater ion absorption is predicted with lower k(sub)||, surprisingly little variation in the tail was observed, along with a small neutron rate enhancement with higher k(sub)||. Data from the neutral particle analyzer, neutron detectors, X-ray crystal spectrometer, and Thomson scattering is presented, along with results from the TRANSP transport analysis code, ray-tracing codes HPRT and CURRAY, full-wave code and AORSA, quasi-linear code CQL3D, and ion loss codes EIGOL and CONBEAM.

  18. Synthetic data products for future H I galaxy surveys: a tool for characterizing source confusion in spectral line stacking experiments

    NASA Astrophysics Data System (ADS)

    Elson, E. C.; Blyth, S. L.; Baker, A. J.

    2016-08-01

    Much of our current understanding of neutral, atomic gas in galaxies comes from radio observations of the nearby Universe. Until the next generation of instruments allow us to push to much higher redshifts, we must rely mostly upon theoretical models of galaxy formation to provide us with key insights into the likely cosmic evolution of HI in the Universe, and its links to molecular clouds and star formation. In this work, we present a new set of methods to convert mock galaxy catalogues into synthetic data cubes containing model galaxies with realistic spatial and spectral HI distributions over large cosmological volumes. Such synthetic data products can be used to guide observing and data handling/analysis strategies for forthcoming HI galaxy surveys. As a demonstration of the potential use of our simulated products we use them to conduct several mock HI stacking experiments for both low and high-redshift galaxy samples. The stacked spectra can be accurately decomposed into contributions from target and non-target galaxies, revealing in all co-added spectra large fractions of contaminant mass due to source confusion. Our results are consistent with similar estimates extrapolated from z=0 observational data. The amount of confused mass in a stacked spectrum grows almost linearly with the size of the observational beam, suggesting potential over-estimates of Omega_HI by some recent HI stacking experiments. Our simulations will allow the study of subtle redshift-dependent effects in future stacking analyses.

  19. Synthetic data products for future H I galaxy surveys: a tool for characterizing source confusion in spectral line stacking experiments

    NASA Astrophysics Data System (ADS)

    Elson, E. C.; Blyth, S. L.; Baker, A. J.

    2016-08-01

    Much of our current understanding of neutral, atomic gas in galaxies comes from radio observations of the nearby Universe. Until the next generation of instruments allow us to push to much higher redshifts, we must rely mostly upon theoretical models of galaxy formation to provide us with key insights into the likely cosmic evolution of H I in the Universe, and its links to molecular clouds and star formation. In this work, we present a new set of methods to convert mock galaxy catalogues into synthetic data cubes containing model galaxies with realistic spatial and spectral H I distributions over large cosmological volumes. Such synthetic data products can be used to guide observing and data handling/analysis strategies for forthcoming H I galaxy surveys. As a demonstration of the potential use of our simulated products we use them to conduct several mock H I stacking experiments for both low and high-redshift galaxy samples. The stacked spectra can be accurately decomposed into contributions from target and non-target galaxies, revealing in all co-added spectra large fractions of contaminant mass due to source confusion. Our results are consistent with similar estimates extrapolated from z = 0 observational data. The amount of confused mass in a stacked spectrum grows almost linearly with the size of the observational beam, suggesting potential overestimates of Ω _{H I} by some recent H I stacking experiments. Our simulations will allow the study of subtle redshift-dependent effects in future stacking analyses.

  20. Characterization of incubation experiments and development of an enrichment culture capable of ammonium oxidation under iron-reducing conditions

    NASA Astrophysics Data System (ADS)

    Huang, S.; Jaffé, P. R.

    2015-02-01

    Incubation experiments were conducted using soil samples from a forested riparian wetland where we have previously observed anaerobic ammonium oxidation coupled to iron reduction. Production of both nitrite and ferrous iron was measured repeatedly during incubations when the soil slurry was supplied with either ferrihydrite or goethite and ammonium chloride. Significant changes in the microbial community were observed after 180 days of incubation as well as in a continuous flow membrane reactor, using 16S rRNA gene PCR-denaturing gradient gel electrophoresis, 454 pyrosequencing, and real-time quantitative PCR analysis. We be Acidimicrobiaceae bacterium A6), belonging to the Acidimicrobiaceae family, whose closest cultivated relative is Ferrimicrobium acidiphilum (with 92% identity) and Acidimicrobium ferrooxidans (with 90% identity), might play a key role in this anaerobic biological process that uses ferric iron as an electron acceptor while oxidizing ammonium to nitrite. After ammonium was oxidized to nitrite, nitrogen loss proceeded via denitrification and/or anammox.

  1. Fabrication and Characterization of Samples for a Material Migration Experiment on the Experimental Advanced Superconducting Tokamak (EAST).

    SciTech Connect

    Wampler, William R.; Van Deusen, Stuart B.

    2015-12-01

    This report documents work done for the ITER International Fusion Energy Organization (Sponsor) under a Funds-In Agreement FI 011140916 with Sandia National Laboratories. The work consists of preparing and analyzing samples for an experiment to measure material erosion and deposition in the EAST Tokamak. Sample preparation consisted of depositing thin films of carbon and aluminum onto molybdenum tiles. Analysis consists of measuring the thickness of films before and after exposure to helium plasma in EAST. From these measurements the net erosion and deposition of material will be quantified. Film thickness measurements are made at the Sandia Ion Beam Laboratory using Rutherford backscattering spectrometry and nuclear reaction analysis, as described in this report. This report describes the film deposition and pre-exposure analysis. Results from analysis after plasma exposure will be given in a subsequent report.

  2. Time-resolved characterization and energy balance analysis of implosion core in shock-ignition experiments at OMEGA

    SciTech Connect

    Florido, R. Mancini, R. C.; Nagayama, T.; Tommasini, R.; Delettrez, J. A.; Regan, S. P.

    2014-10-15

    Time-resolved temperature and density conditions in the core of shock-ignition implosions have been determined for the first time. The diagnostic method relies on the observation, with a streaked crystal spectrometer, of the signature of an Ar tracer added to the deuterium gas fill. The data analysis confirms the importance of the shell attenuation effect previously noted on time-integrated spectroscopic measurements of thick-wall targets [R. Florido et al., Phys. Rev. E 83, 066408 (2011)]. This effect must be taken into account in order to obtain reliable results. The extracted temperature and density time-histories are representative of the state of the core during the implosion deceleration and burning phases. As a consequence of the ignitor shock launched by the sharp intensity spike at the end of the laser pulse, observed average core electron temperature and mass density reach T ∼ 1100 eV and ρ ∼ 2 g/cm{sup 3}; then temperature drops to T ∼ 920 eV while density rises to ρ ∼ 3.4 g/cm{sup 3} about the time of peak compression. Compared to 1D hydrodynamic simulations, the experiment shows similar maximum temperatures and smaller densities. Simulations do not reproduce all observations. Differences are noted in the heating dynamics driven by the ignitor shock and the optical depth time-history of the compressed shell. Time-histories of core conditions extracted from spectroscopy show that the implosion can be interpreted as a two-stage polytropic process. Furthermore, an energy balance analysis of implosion core suggests an increase in total energy greater than what 1D hydrodynamic simulations predict. This new methodology can be implemented in other ICF experiments to look into implosion dynamics and help to understand the underlying physics.

  3. Characterization of heat transport dynamics in laser-produced plasmas using collective Thomson scattering: Simulation and proposed experiment

    SciTech Connect

    Cameron, S.M.; Camacho, J.F.

    1995-12-01

    The authors propose an experiment in which the collective Thomson scattering lineshape obtained from ion acoustic waves is used to infer the spatial structure of local heat transport parameters and collisionality in a laser-produced plasma. The peak-height asymmetry in the ion acoustic wave spectrum will be used in conjunction with a recently developed model describing the effects of collisional and Landau damping contributions on the low-frequency electron density fluctuation spectrum to extract the relative electron drift velocity. This drift arises from temperature gradients in the plasma. The local heat flux, which is proportional to the drift, can then be estimated, and the electron thermal conductivity will be inferred from the relationship between the calculated heat flux and the experimentally determined temperature gradient. Damping of the entropy wave component at zero mode frequency is shown to be an estimate of the ion thermal conductivity, and its visibility is a direct measure of the ion-ion mean free path. The authors also propose to measure thermal transport parameters under dynamic conditions in which the plasma is heated impulsively by a laser beam on a fast ({approximately}50 ps) time scale. This technique will enable the authors to study heat transport in the presence of the large temperature gradients that are generated by this local heating mechanism. Deviations of the inferred local thermal conductivity from its Spitzer-Haerm value can be used to study the transition to the nonlocal heat transport regime. The authors have constructed a simple numerical model of this proposed experiment and present the results of a simulation. 41 refs., 9 figs.

  4. Characterization of water dissociation on α-Al2O3(11[combining macron]02): theory and experiment.

    PubMed

    Wirth, Jonas; Kirsch, Harald; Wlosczyk, Sebastian; Tong, Yujin; Saalfrank, Peter; Campen, R Kramer

    2016-06-01

    The interaction of water with α-alumina (i.e. α-Al2O3) surfaces is important in a variety of applications and a useful model for the interaction of water with environmentally abundant aluminosilicate phases. Despite its significance, studies of water interaction with α-Al2O3 surfaces other than the (0001) are extremely limited. Here we characterize the interaction of water (D2O) with a well defined α-Al2O3(11[combining macron]02) surface in UHV both experimentally, using temperature programmed desorption and surface-specific vibrational spectroscopy, and theoretically, using periodic-slab density functional theory calculations. This combined approach makes it possible to demonstrate that water adsorption occurs only at a single well defined surface site (the so-called 1-4 configuration) and that at this site the barrier between the molecularly and dissociatively adsorbed forms is very low: 0.06 eV. A subset of OD stretch vibrations are parallel to this dissociation coordinate, and thus would be expected to be shifted to low frequencies relative to an uncoupled harmonic oscillator. To quantify this effect we solve the vibrational Schrödinger equation along the dissociation coordinate and find fundamental frequencies red-shifted by more than 1500 cm(-1). Within the context of this model, at moderate temperatures, we further find that some fraction of surface deuterons are likely delocalized: dissociatively and molecularly absorbed states are no longer distinguishable. PMID:27109875

  5. Characterization of the nonlinear elastic properties of soft tissues using the supersonic shear imaging (SSI) technique: inverse method, ex vivo and in vivo experiments.

    PubMed

    Jiang, Yi; Li, Guo-Yang; Qian, Lin-Xue; Hu, Xiang-Dong; Liu, Dong; Liang, Si; Cao, Yanping

    2015-02-01

    Dynamic elastography has become a new clinical tool in recent years to characterize the elastic properties of soft tissues in vivo, which are important for the disease diagnosis, e.g., the detection of breast and thyroid cancer and liver fibrosis. This paper investigates the supersonic shear imaging (SSI) method commercialized in recent years with the purpose to determine the nonlinear elastic properties based on this promising technique. Particularly, we explore the propagation of the shear wave induced by the acoustic radiation force in a stressed hyperelastic soft tissue described via the Demiray-Fung model. Based on the elastodynamics theory, an analytical solution correlating the wave speed with the hyperelastic parameters of soft tissues is first derived. Then an inverse approach is established to determine the hyperelastic parameters of biological soft tissues based on the measured wave speeds at different stretch ratios. The property of the inverse method, e.g., the existence, uniqueness and stability of the solution, has been investigated. Numerical experiments based on finite element simulations and the experiments conducted on the phantom and pig livers have been employed to validate the new method. Experiments performed on the human breast tissue and human heel fat pads have demonstrated the capability of the proposed method for measuring the in vivo nonlinear elastic properties of soft tissues. Generalization of the inverse analysis to other material models and the implication of the results reported here for clinical diagnosis have been discussed. PMID:25476413

  6. Characterization of marine boundary layer aerosol from North Atlantic and European sources: Physical and chemical properties and climate forcing parameters

    NASA Astrophysics Data System (ADS)

    Dusek, Ulrike

    This thesis focuses on aerosol properties measured in Southwestern Portugal during the second Aerosol Characterization Experiment. Fundamental aerosol physical properties such as particle size distribution and hygroscopic properties are related to possible sources and aerosol transformation processes. From these fundamental properties we derive aerosol properties that are important for aerosol forcing of climate. First, a new method for calculating CCN spectra is proposed in this work and tested using sensitivity studies and comparisons to direct measurements. The measured and calculated CCN spectra differ on average by 30%, which at small supersaturations is similar to the measurement uncertainties. Second, aerosol number to volume ratios (R) are calculated and the fact that values of R are relatively constrained is explained based on observed correlations between size distribution parameters. Third, a simple parameterization of the humidity dependence of the submicron aerosol scattering coefficient has been derived, depending only on a volume weighted average diameter growth factor and the volume mean diameter of the dry size distribution. One set of empirical parameters can be used to parameterize all aerosol types characterized during the ACE-2 measurement period. Aerosol physical properties and climate forcing parameters in the North-East Atlantic Ocean were clearly affected by pollution outbreaks from Europe. The submicron particle volume increased by a factor of 5 in polluted conditions, the light scattering coefficient of dry particles increased on average by a factor of up to 10, CCN concentrations at supersaturations of 0.2% increased by a factor of 3--5. The aerosol fundamental properties vary often strongly with air mass history, but also show short-term variability that often has a characteristic diurnal scale. The number concentration of fine particles below 50nm and the particle hygroscopic growth factors are mostly dominated by diurnal processes

  7. Characterization of neoformed illite from hydrothermal experiments at 250 C and P{sub v,soln}: An HRTEM/ATEM study

    SciTech Connect

    Yates, D.M.; Rosenberg, P.E.

    1998-11-01

    Solid products from hydrothermal experiments conducted at 250 C and P{sub v,soln} were characterized by powder X-ray diffraction (XRD) and ATEM/HRTEM. Experiments were conducted with muscovite, kaolinite, and quartz or amorphous silica in 2M KCl solutions for 43 to 176 d. Post-experiment solution compositions lie either within the illite(0.88 K) stability field or on the illite(0.88 K)-kaolinite or illite(0.88 K)-diaspore univariant boundaries in log (a{sub K}{sup +}/a{sub H}{sup +}) vs. log a{sub H{sub 4}SiO{sub 4}} activity space. Transmission electron microscopy (TEM) observations of muscovite grain edges reveal the neoformation of illite crystals with a range of compositions (ATEM) from 0.31 to 0.89 K/O{sub 10}(OH){sub 2}. The range of K-contents appears to narrow toward 0.88 K/O{sub 10}(OH){sub 2} with increased experiment duration. HRTEM suggests the presence of 2 to 11 layer fundamental particles composed of illitic layers with 10 A periodicity. Fundamental particle thicknesses increase toward an average of 8 layers/particle with increased experiment duration. In the longer duration experiments, fundamental particle thicknesses were normally distributed about thicknesses of 4 and 8 layers, whereas fundamental particles with thicknesses <4 layers were common in a shorter duration experiment. The compositions and structure of the illites are consistent with the multiphase model, which states that the smectite-to-illite transition occurs through the step-wise formation of solubility-controlling phases consisting of fundamental particles with thicknesses of 1, 2, 4, and {le}8 layers. The increase in K-content and fundamental particle thickness with the extent of reaction suggests that the illite crystals underwent a prograde reaction culminating in the formation of end-member illite [0.88 K/O{sub 10}(OH){sub 2}]. This reaction, in conjunction with the previously observed, retrograde reaction from muscovite to end-member illite, demonstrates the stability of end

  8. Characterization of incubation experiments and development of an enrichment culture capable of ammonium oxidation under iron reducing conditions

    NASA Astrophysics Data System (ADS)

    Huang, S.; Jaffé, P. R.

    2014-08-01

    Incubation experiments were conducted using soil samples from a forested riparian wetland where we have previously observed anaerobic ammonium oxidation coupled to iron reduction. Production of both nitrite and ferrous iron were measured repeatedly during incubations when the soil slurry was supplied with either ferrihydrite or goethite and ammonium chloride. Significant changes in the microbial community were observed after 180 days of incubation as well as in a continuous flow membrane reactor, using 16S rRNA gene PCR-denaturing gradient gel electrophoresis, 454-pyrosequencing, and real-time quantitative PCR analysis. We believe that one of the dominant microbial species in our system (an uncultured Acidimicrobiaceae bacterium A6), belonging to the Acidimicrobiaceae family, whose closest cultivated relative is Ferrimicrobium acidiphilum (with 92% identity) and Acidimicrobium ferrooxidans (with 90% identity), might play a key role in this anaerobic biological process that uses ferric iron as an electron acceptor while oxidizing ammonium to nitrite. After ammonium was oxidized to nitrite, nitrogen loss proceeded via denitrification and/or anammox.

  9. Characterization and correction of Global Ozone Monitoring Experiment 2 ultraviolet measurements and application to ozone profile retrievals

    NASA Astrophysics Data System (ADS)

    Cai, Zhaonan; Liu, Yi; Liu, Xiong; Chance, Kelly; Nowlan, Caroline R.; Lang, Ruediger; Munro, Rosemary; Suleiman, Raid

    2012-04-01

    We present an assessment study of the Global Ozone Monitoring Experiment 2 (GOME-2) reflectance for the wavelength range 270-350 nm by comparing measurements with simulations calculated using the vector linearized discrete ordinate radiative transfer model (VLIDORT) and Microwave Limb Sounder (MLS) ozone profiles. The results indicate wavelength- and cross-track-position-dependent biases. GOME-2 reflectance is overestimated by 10% near 300 nm and by 15%-20% around 270 nm. Stokes fraction measurements made by onboard polarization measurement devices are also validated directly using the VLIDORT model. GOME-2 measurements agree well with the simulated Stokes fractions, with mean biases ranging from -1.0% to ˜2.9%; the absolute differences are less than 0.05. Cloudiness-dependent biases suggest the existence of uncorrected stray-light errors that vary seasonally and latitudinally. Temporal analysis indicates that reflectance degradation began at the beginning of the mission; the reflectance degrades by 15% around 290 nm and by 2.2% around 325 nm from 2007 through 2009. Degradation shows wavelength- and viewing-angle-dependent features. Preliminary validation of ozone profile retrievals with MLS, Michelson Interferometer for Passive Atmospheric Sounding, and ozonesonde reveals that the application of radiometric recalibration improves the ozone profile retrievals as well as reduces fitting residuals by 30% in band 2b.

  10. Use of Rainfall Simulation and Concentrated Flow Experiments to Characterize Rangeland Hydrologic and Erosional Processes in the Great Basin, USA

    NASA Astrophysics Data System (ADS)

    Pierson, Fred; Robichaud, Pete; Williams, Jason; Kormos, Patrick; Al-Hamdan, Osama; Boll, Jan

    2010-05-01

    Much of what is known about rangeland hydrology comes from short-term, poorly replicated studies of small (0.25 - 1 m2) field plots and personal observations of hydrologic behavior on gently sloping semi-arid shrub and grassland sites. Historical rangeland models have been largely based on these limited datasets and inferences from crop land studies. In recent years, we have sought to expand the inference space through the use of rainfall simulations and concentrated flow experiments from the small to large (13.5 - 32.5 m2) plot scales on steeply sloping, disturbed and undisturbed rangeland ecosystems. We present an overview of one such multi-year rainfall simulation study and highlight how the methodology and results specifically address current voids in the understanding of hydrology and erosion processes from steeply sloping, semi-arid rangeland landscapes. We further demonstrate how the methodologies and aggregated results from studies across a range of plant communities and degrees of disturbance reveal consistent trends in runoff and erosional behavior. The aggregated results advance the understanding of rangeland hydrology and erosion processes and present a well replicated dataset from which hydrologic model parameters can be derived.

  11. Functional characterization of the apple MhGAI1 gene through ectopic expression and grafting experiments in tomatoes.

    PubMed

    Wang, Shuang-Shuang; Liu, Ze-Zhou; Sun, Chao; Shi, Qing-Hua; Yao, Yu-Xin; You, Chun-Xiang; Hao, Yu-Jin

    2012-02-15

    DELLA proteins are essential components of GA signal transduction. MhGAI1 was isolated from the tea crabapple (Malus hupehensis Redh. var. pingyiensis), and it was found to encode a DELLA protein. Mhgai1 is a GA-insensitive allele that was artificially generated via a bridge-PCR approach. Ectopic expression of Mhgai1 reduced plant stature, decreased spontaneous fruit-set-ratio and enhanced drought-tolerance in transgenic tomatoes. In addition, we examined the long-distance movement of Mhgai1 mRNAs by grafting experiments and SqRT-PCR analysis. It was found that the wild-type scions accumulated Mhgai1 transcripts trafficked from the transgenic rootstocks and therefore exhibited dwarf phenotypes. Furthermore, transgenic tomato plants produced more soluble solids, sugars and organic acids compared to wild-type tomatoes, suggesting an involvement of GA signaling in the regulation of fruit quality. Despite noticeable accumulation in the leaves and stems of WT scions, Mhgai1 transcripts were undetectable in flowers and fruit. Therefore, fruit quality was less influenced by the grafting of WT scions onto transgenic rootstocks than they were by the ectopic expression of Mhgai1 in transgenic rootstocks. Taken together, MhGAI1, which functions as a repressor in the GA signaling pathway, and its GA-insensitive allele, Mhgai1, could turn out to be useful targets for the genetic improvement of dwarfing rootstocks in apples. PMID:22153898

  12. A characterization of check valve degradation and failure experience in the nuclear power industry: 1984-1990. Volume 1

    SciTech Connect

    Casada, D.A.; Todd, M.D.

    1993-09-01

    Check valve operating problems in recent years have resulted in significant operating transients, increased cost and decreased system availability. As a result, additional attention has beau given to check valves by utilities (resulting in the formation of the Nuclear Industry Check Valve Group), as well as the US Nuclear Regulatory Commission and the American Society of Mechanical Engineers Operation and Maintenance Committee. All these organizations have the fundamental goal of ensuring reliable operation of check valves. A key ingredient to an engineering-oriented reliability improvement effort is a thorough understanding of relevant historical experience. A detailed review of historical failure data, available through the Institute of Nuclear Power Operation`s Nuclear Plant Reliability Data System, has been conducted. The focus of the review is on check valve failures that have involved significant degradation of the valve internal parts. A variety of parameters are considered, including size, age, system of service, method of failure discovery, the affected valve parts, attributed causes, and corrective actions.

  13. WE-G-BRF-08: Robust Characterization and Patient Experience of a Time-Resolved Ultrasound System for Prostate Tracking

    SciTech Connect

    Sandhu, R; Yan, D; Ionascu, D

    2014-06-15

    Purpose: Characterization of a time resolved ultrasound device (4D-US), used for localization and motion monitoring of prostate radiotherapy. Methods: The 4D-US system accuracy to reproduce prostate motion was investigated and artifacts were determined under detailed experimental and ultimately clinical conditions. Two fields of view (FOV) of 30 and 60 degrees and two corresponding acquisition speeds were evaluated under experimental conditions with gradual increased complexity. The reconstruction ability and trajectory tracking depends on the relative speed between the ultrasound probe and the moving object. Two different types of phantoms were developed; one capable of following a predetermined trajectory with variable speeds, and a second one designed to test the US ability to a track dynamically-deformed prostate. Four hypo-prostate patients (10fx) were monitored during the treatment to detect intrafraction motion. Results: Prostate speeds up to 6mm/s and 3mm/s can be successfully tracked by the system when using the small and large FOV, respectively. The system response time, calculated based on the speedinduced volumetric distortion, is dependent on the FOV, with 1.1s for the small FOV and 1.4s for the large FOV. The response time was also found dependent on the size of target, increasing from 1.4s to 1.7s for target diameters of 4cm to 2.8cm. The deformable dynamic phantom has shown that the system is able to successfully follow a highly-deformed, slowmoving prostate with an error < 0.5mm. The overall range of patient intrafraction motion was (−2.4 to 2.2)mm, (−2.6 to 2.4)mm, (−3.0 to 4.3)mm in the RL, SI and AP directions, respectively. Conclusion: The 4DUS system using a large FOV is appropriate to monitor the prostate motion as well as the adjacent organs at risk. However, infrequent abrupt motion can be problematic and the small FOV system is preferred for such patients, particularly for future applications as online prostate monitoring employed

  14. Hydrologic characterization of desert soils with varying degrees of pedogenesis: 1. field experiments evaluating plant-relevant soil water behavior

    USGS Publications Warehouse

    Nimmo, J.R.; Perkins, K.S.; Schmidt, K.M.; Miller, D.M.; Stock, J.D.; Singha, K.

    2009-01-01

    To assess the eff ect of pedogenesis on the soil moisture dynamics infl uencing the character and quality of ecological habitat, we conducted infi ltration and redistribution experiments on three alluvial deposits in the Mojave National Preserve: (i) recently deposited active wash sediments, (ii) a soil of early Holocene age, and (iii) a highly developed soil of late Pleistocene age. At each, we ponded water in a 1-m-diameter infi ltration ring for 2.3 h and monitored soil water content and matric pressure during and atier infi ltration, using probes and electrical resistivity imaging (ERI). Infi ltration and downward fl ow rates were greater in younger material, favoring deep-rooted species. Deep-rooted species tend to colonize the margins of washes, where they are unaff ected by sediment transport that inhibits colonization. The ERI results support important generalizations, for example that shallower than 0.5 m, infi ltrated water persists longer in highly developed soil, favoring shallow-rooted species. Soil moisture data for the two youngest soils suggested that saturation overshoot, which may have signifi cant but unexplored hydroecologic and pedogenic eff ects, occurred at the horizontally advancing weting front. Spatial heterogeneity of soil properties generally increased with pedogenic development. Evidence suggested that some early-stage developmental processes may promote uniformity; the intermediate- age soil appeared to have the least heterogeneity in terms of textural variation with depth, and also the least anisotropy. Lateral heterogeneity was pronounced in older soil, having a multitude of eff ects on the distribution and retention of soil water, and may facilitate certain water-conserving strategies of plants over what would be possible in a laterally homogeneous soil. ?? Soil Science Society of America.

  15. In situ and real time characterization of interface microstructure in 3D alloy solidification: benchmark microgravity experiments in the DECLIC-Directional Solidification Insert on ISS

    NASA Astrophysics Data System (ADS)

    Ramirez, A.; Chen, L.; Bergeon, N.; Billia, B.; Gu, Jiho; Trivedi, R.

    2012-01-01

    Dynamical microstructure formation and selection during solidification processing, which has a major influence on the properties in the use of elaborated materials, occur during the growth process. In situ observation of the solid-liquid interface morphology evolution is thus necessary. On earth, convection effects dominate in bulk samples and may strongly interact with microstructure dynamics and alter pattern characterization. Series of solidification experiments with 3D cylindrical sample geometry were conducted in succinonitrile (SCN) -0.24 wt%camphor (model transparent system), in microgravity environment in the Directional Solidification Insert of the DECLIC facility of CNES (French space agency) on the International Space Station (ISS). Microgravity enabled homogeneous values of control parameters over the whole interface allowing the obtaining of homogeneous patterns suitable to get quantitative benchmark data. First analyses of the characteristics of the pattern (spacing, order, etc.) and of its dynamics in microgravity will be presented.

  16. (Cd,Mn)Te detectors for characterization of x-ray emissions generated during laser-driven fusion experiments

    SciTech Connect

    Cross,A.S.; Knauer, J. P.; Mycielski, A.; Kochanowska, D.; Wiktowska-Baran, M.; Jakiela, R.; Domagala, J.; Cui, Y.; James, R.; Sobolewski, R.

    2008-10-19

    We present our measurements of (Cd,Mn)Te photoconductive detectors (PCDs), fabricated for the goal of measuring both the temporal and spectral dependences of X-ray emissions generated from laser-illuminated targets during the inertial confinement fusion experiments. Our Cd{sub 1-x}Mn{sub x}Te (x = 0.05) single crystals, doped with V, were grown using a vertical Bridgman method and, subsequently, annealed in Cd for the highest resistivity ({approx}10{sup 10} {Omega}cm) and a good mobility-lifetime product ({approx}10{sup -3} cm{sup 2}/V). The 1-mm- and 2.3-mm-thick detectors were placed in the same housing as two 1-mm-thick diamond PCDs. All devices were pre-screened by a 7.6-mm-thick Be X-ray filter with a frequency cutoff of 1 keV. The incident shots from the OMEGA laser were 1-ns-long square pulses with energies ranging from 2.3 kJ to 22.6 kJ, and the PCDs were biased with 5000 V/cm. The response amplitudes and rise times of our (Cd,Mn)Te PCDs were comparable with the diamond detector performance, while the decay times were 4 to 10 times longer and in the 2-5 ns range. We observed two X-ray emission events separated by 1.24 ns. The first was identified as caused by heating of the target and creating a hot corona, while the second one was from the resulting compressed core. For comparison purposes, our testing was performed using {approx}1 keV X-ray photons, optimal for the diamond PCD. According to the presented simulations, however, at X-ray energies >10 keV diamond absorption efficiency drops to <50%, whereas for (Cd,Mn)Te the drop occurs at {approx}100 keV with near perfect, 100% absorption, up to 50 keV.

  17. NSF Research Experience for Undergraduates: 3-D Characterization and Comparison of Vegetation Structure in a Tropical Premontane Wet Forest

    NASA Astrophysics Data System (ADS)

    Washington-Allen, R. A.; Davis, K.; Falkowski, T.; Tarbox, B.; Delgado, A.; March, R.; Moore, G. W.; Tjoelker, M.; Gonzalez, E.; Houser, C.

    2011-12-01

    This National Science Foundation (NSF) Research Experience for Undergraduates (REU) site hosted by Texas A&M University to allow selected undergraduate students to conduct original research on various aspects of the ecohydrology of understudied tropical pre-montane forest at the Texas A&M Soltis Center for Research and Education in Central Costa Rica. This particular study was conducted by 3 students under the guidance of 5 mentors who assisted in experimental design, equipment use, maintenance, and training, plant species identification and other logistical requirements. The goal of this study was to determine the change in 3-D structure of tropical premontane wet forest under 3 different land uses: a carbon tree farm, secondary logged forest, and primary unlogged forest. Traditional forest mensuration techniques including glass prisms (stand basal area), laser clinometers (height), vertical and horizontal PAR, spherical densiometers, and hemispherical photography (GAP fraction and LAI) and, root cores, and diameter-at-breast height (DBH) tapes were acquired in one 30-m diameter plot per land and use compared to similar metrics collected by a terrestrial scanning laser (TSL) and ground penetrating radar (GPR) at 400 MHz and 1.5 GHz across 3 land uses along an elevation gradient from ~380-masl to 525-masl. Besides discovery, another goal of this study was to see if the TSL and GPR can help meet the Tier 2 and 3 monitoring and verification goals of the United Nations Collaborative Programme on Reducing Emissions from Deforestation and Forest Degradation in Developing Countries for estimating above- and below-ground biomass using remote sensing. This is of particular importance because the GPR may be able to capture below-ground biomass in a more efficient manner than traditional coring and the TSL and GPR can capture data on highly sloped terrain where both airborne and satellite RADAR and LIDAR are limited. The 50, 000 pts/sec 532 nm TSL collected 3 to 5 scans per

  18. High resolution Raman lidar measurements for the characterization of the water vapour inflow in the frame of the Hydrological Cycle in the Mediterranean Experiment

    NASA Astrophysics Data System (ADS)

    Di Girolamo, Paolo; Cacciani, Marco; Stelitano, Dario; Summa, Donato

    2013-04-01

    The University of BASILicata Raman Lidar system (BASIL) was deployed in Candillargues (Southern France, Lat: 43°37' N, Long: 4° 4' E) in the frame of the Hydrological Cycle in the Mediterranean Experiment - HyMeX. Within this experiment a major field campaign (Special Observation Period 1-SOP1, September to November 2012) took place over the Northwestern Mediterranean Sea and its surrounding coastal regions in France, Italy and Spain, with a specific focus on the study of heavy precipitation and flash-flood events. During HyMeX-SOP1, BASIL operated between 5 September and 5 November 2012, collecting more than 600 hours of measurements, distributed over 51 measurement days and 19 intensive observation periods (IOPs). The major feature of BASIL is represented by its capability to perform high-resolution and accurate measurements of atmospheric temperature and water vapour, both in daytime and night-time, based on the application of the rotational and vibrational Raman lidar techniques in the UV (Di Girolamo et al., 2004, 2006, 2009). This makes it an ideal tool for the characterization of the water vapour inflow in Southern France, which is important piece of information to improve the comprehension and forecasting capabilities of heavy precipitations in the Northwestern Mediterranean basin. Preliminary measurements from this field deployment will be illustrated and discussed at the Conference. These measurements allow to monitor and characterize the marine atmospheric flow that transport moist and conditionaly unstable air towards the coasts, which is feeding into the HPE events in Southern France. Measurements from BASIL can also be used to better characterize Planetary Boundary Layer moisture transport mechanisms from the surface to deep-convection systems. Besides temperature and water vapour, BASIL also provides measurements of the particle (aerosol/cloud) backscattering coefficient at 355, 532 and 1064 nm, of the particle extinction coefficient at 355 and 532

  19. Design and characterization of the SiPM tracking system of NEXT-DEMO, a demonstrator prototype of the NEXT-100 experiment

    NASA Astrophysics Data System (ADS)

    Álvarez, V.; Ball, M.; Borges, F. I. G.; Cárcel, S.; Castel, J.; Cebrián, S.; Cervera, A.; Conde, C. A. N.; Dafni, T.; Dias, T. H. V. T.; Díaz, J.; Egorov, M.; Esteve, R.; Evtoukhovitch, P.; Fernandes, L. M. P.; Ferrario, P.; Ferreira, A. L.; Freitas, E. D. C.; Garcia, A. N. C.; Gehman, V. M.; Gil, A.; Goldschmidt, A.; Gómez, H.; Gómez-Cadenas, J. J.; González-Díaz, D.; Gutiérrez, R. M.; Hauptman, J.; Hernando Morata, J. A.; Herrera, D. C.; Iguaz, F. J.; Irastorza, I. G.; Jinete, M. A.; Labarga, L.; Laing, A.; Liubarsky, I.; Lopes, J. A. M.; Lorca, D.; Losada, M.; Luzón, G.; Marí, A.; Martín-Albo, J.; Martínez, A.; Miller, T.; Moiseenko, A.; Monrabal, F.; Monteiro, C. M. B.; Mora, F. J.; Moutinho, L. M.; Muñoz Vidal, J.; Natal da Luz, H.; Navarro, G.; Nebot-Guinot, M.; Nygren, D.; Oliveira, C. A. B.; Palma, R.; Pérez, J.; Pérez Aparicio, J. L.; Renner, J.; Ripoll, L.; Rodríguez, A.; Rodríguez, J.; Santos, F. P.; dos Santos, J. M. F.; Seguí, L.; Serra, L.; Shuman, D.; Simón, A.; Sofka, C.; Sorel, M.; Toledo, J. F.; Tomás, A.; Torrent, J.; Tsamalaidze, Z.; Vázquez, D.; Veloso, J. F. C. A.; Villar, J. A.; Webb, R.; White, J. T.; Yahlali, N.

    2013-05-01

    NEXT-100 experiment aims at searching the neutrinoless double-beta decay of the 136Xe isotope using a TPC filled with a 100 kg of high-pressure gaseous xenon, with 90% isotopic enrichment. The experiment will take place at the Laboratorio Subterr&aposaneo de Canfranc (LSC), Spain. NEXT-100 uses electroluminescence (EL) technology for energy measurement with a resolution better than 1% FWHM. The gaseous xenon in the TPC additionally allows the tracks of the two beta particles to be recorded, which are expected to have a length of up to 30 cm at 10 bar pressure. The ability to record the topological signature of the ββ0ν events provides a powerful background rejection factor for the ββ experiment. In this paper, we present a novel 3D imaging concept using SiPMs coated with tetraphenyl butadiene (TPB) for the EL read out and its first implementation in NEXT-DEMO, a large-scale prototype of the NEXT-100 experiment. The design and the first characterization measurements of the NEXT-DEMO SiPM tracking system are presented. The SiPM response uniformity over the tracking plane drawn from its gain map is shown to be better than 4%. An automated active control system for the stabilization of the SiPMs gain was developed, based on the voltage supply compensation of the gain drifts. The gain is shown to be stabilized within 0.2% relative variation around its nominal value, provided by Hamamatsu, in a temperature range of 10°C. The noise level from the electronics and the SiPM dark noise is shown to lay typically below the level of 10 photoelectrons (pe) in the ADC. Hence, a detection threshold at 10 pe is set for the acquisition of the tracking signals. The ADC full dynamic range (4096 channels) is shown to be adequate for signal levels of up to 200 pe/μs, which enables recording most of the tracking signals.

  20. Molecular characterization of a cometary nucleus composition with the gas chromatograph-mass spectrometer of the COSAC experiment onboard the Philae lander of the Rosetta mission

    NASA Astrophysics Data System (ADS)

    Szopa, Cyril; Gomes, Ricardo; Raulin, Francois; Sternberg, Robert; Coscia, David; Cabane, Michel; Meierhenrich, Uwe; Gautier, Thomas; Goesmann, Fred; Cosac Team

    2014-05-01

    One among the main goal of the Rosetta mission is to characterize the physical and chemical properties of the comet P46/Churyumov-Gerasimenko nucleus. With this aim, the mission will offer for the first time the capability to achieve in situ measurements at the cometary surface with the Philae lander. This characterization is all the more important that the properties of cometary nuclei surfaces are almost unknown whereas it is the source of the processes taking place in the cometary comae and tails. In this frame, the determination of the cometary nucleus molecular composition is of primary importance as it would allow to : i. give clues on the relationship between the molecules present in the nucleus and those detected from observations ; ii. determine the importance of comets in the delivery of inorganic and organic molecules to planetary surfaces ; iii. improve our knowledge of the connection between comets and materials present in the interstellar medium. The COmetary SAmpling and Composition experiment will be the molecular analyzer onboard the Philae lander. It is constituted of a solid sampler, a gas chromatograph and a mass spectrometer, that allow to analyze volatile compounds coming from both the cometary atmosphere and samples collected in the cometary regolith. In order to prepare the analysis and interpretation of the data to be collected after the landing of Philae, a series of calibration and tests were done in laboratory with the COSAC spare model or spare components of the GC. These were done in order to evaluate the health state of the gas chromatograph after almost 10 years spent in the interplanetary environment, and also to estimate the analytical performances of the instrument under realistic operation conditions to be used at the cometary surface. This contribution presents the results of these tests that will be usefull for the COSAC data analysis.

  1. (14)C-labeled organic amendments: Characterization in different particle size fractions and humic acids in a long-term field experiment.

    PubMed

    Tatzber, Michael; Stemmer, Michael; Spiegel, Heide; Katzlberger, Christian; Landstetter, Claudia; Haberhauer, Georg; Gerzabek, Martin H

    2012-05-01

    Knowledge about the stabilization of organic matter input to soil is essential for understanding the influence of different agricultural practices on turnover characteristics in agricultural soil systems. In this study, soil samples from a long-term field experiment were separated into silt- and clay-sized particles. In 1967, (14)C labeled farmyard manure was applied to three different cropping systems: crop rotation, monoculture and permanent bare fallow. Humic acids (HAs) were extracted from silt- and clay-sized fractions and characterized using photometry, mid-infrared and fluorescence spectroscopy. Remaining (14)C was determined in size fractions as well as in their extracted HAs. Yields of carbon and remaining (14)C in HAs from silt-sized particles and Corg in clay-sized particles decreased significantly in the order: crop rotation > monoculture ≫ bare fallow. Thus, crop rotation not only had the largest overall C-pool in the experiment, but it also best stabilized the added manure. Mid-infrared spectroscopy could distinguish between HAs from different particle size soil fractions. With spectroscopic methods significant differences between the cropping systems were detectable in fewer cases compared to quantitative results of HAs (yields, (14)C, Corg and Nt). The trends, however, pointed towards increased humification of HAs from bare fallow systems compared to crop rotation and monoculture as well as of HAs from clay-sized particles compared to silt-sized particles. Our study clearly shows that the largest differences were observed between bare fallow on one hand and monoculture and crop rotation on the other. PMID:23482702

  2. Characterization of Turbulent Processes by the Raman Lidar System Basil in the Frame of the HD(CP)2 Observational Prototype Experiment - Hope

    NASA Astrophysics Data System (ADS)

    Di Girolamo, Paolo; Summa, Donato; Stelitano, Dario; Cacciani, Marco; Scoccione, Andrea; Behrendt, Andreas; Wulfmeyer, Volker

    2016-06-01

    Measurements carried out by the Raman lidar system BASIL are reported to demonstrate the capability of this instrument to characterize turbulent processes within the Convective Boundary Layer (CBL). In order to resolve the vertical profiles of turbulent variables, high resolution water vapour and temperature measurements, with a temporal resolution of 10 sec and a vertical resolution of 90 and 210 m, respectively, are considered. Measurements of higher-order moments of the turbulent fluctuations of water vapour mixing ratio and temperature are obtained based on the application of spectral and auto-covariance analyses to the water vapour mixing ratio and temperature time series. The algorithms are applied to a case study (IOP 5, 20 April 2013) from the HD(CP)2 Observational Prototype Experiment (HOPE), held in Central Germany in the spring 2013. The noise errors are demonstrated to be small enough to allow the derivation of up to fourth-order moments for both water vapour mixing ratio and temperature fluctuations with sufficient accuracy.

  3. Ground based characterization of biomass burning aerosols during the South American Biomass Burning Analysis (SAMBBA) field experiment in Brazil during Sept - Oct 2012

    NASA Astrophysics Data System (ADS)

    Artaxo, Paulo; Ferreira de Brito, Joel; Varanda Rizzo, Luciana; Johnson, Ben; Haywood, Jim; Longo, Karla; Freitas, Saulo; Coe, Hugh

    2013-04-01

    Biomass burning is one of the major drivers for atmospheric composition in the Southern hemisphere. In Amazonia, deforestation rates have been steadily decreasing, from 27,000 Km² in 2004 to about 5,000 Km² in 2011. This large reduction (by factor 5) was not followed by similar reduction in aerosol loading in the atmosphere due to the increase in agricultural fires. AERONET measurements from 5 sites show a large year-to year variability due to climatic and socio-economic issues. Besides this strong reduction in deforestation rate, biomass burning emissions in Amazonia increases concentrations of aerosol particles, CO, ozone and other species, and also change the surface radiation balance in a significant way. To complement the long term biomass burning measurements in Amazonia, it was organized in 2012 the intensive campaign of the South American Biomass Burning Analysis (SAMBBA) experiment with an airborne and a ground based components. A sampling site was set up at Porto Velho, with measurements of aerosol size distribution, optical properties such as absorption and scattering at several wavelengths, organic aerosol characterization with an ACSM - Aerosol Chemical Speciation Monitor. CO, CO2 and O3 were also measured to characterize combustion efficiency and photochemical processes. Filters for trace elements measured by XRF and for OC/EC determined using a Sunset instrument were also collected. An AERONET CIMEL sunphotometer was operated in parallel with a multifilter radiometer (MFR). A large data set was collected from August to October 2012. PM2.5 aerosol concentrations up to 250 ug/m3 were measured, with up to 20 ug/m3 of black carbon. Ozone went up to 60 ppb at mid-day in August. At night time ozone was consumed completely most of the time. ACSM shows that more than 85% of the aerosol mass was organic with a clear diurnal pattern. The organic aerosol volatility was very variable depending on the air mass sampled over Porto Velho. Aerosol optical depth at

  4. Petrophysical characterization of first ever drilled core samples from an active CO2 storage site, the German Ketzin Pilot Site - Comparison with long term experiments

    NASA Astrophysics Data System (ADS)

    Zemke, Kornelia; Liebscher, Axel

    2014-05-01

    Stuttgart Formation it is difficult to estimate definite CO2 induced changes from petrophysical measurements. The observed changes are only minor. Several batch experiments on Ketzin samples drilled prior injection confirm the results from investigation of the in-situ rock cores. Core samples of the pre-injection wells were exposed to CO2 and brine in autoclaves over various time periods. Samples were characterized prior to and after the experiments by NMR and Mercury Injection Porosimetry (MIP). The results are consistent with the logging data and show only minor change. Unfortunately, also in these experiments observed mineralogical and petrophysical changes were within the natural heterogeneity of the Ketzin reservoir and precluded unequivocal conclusions. However, given the only minor differences between post-injection well and pre-injection well, it is reasonable to assume that the potential dissolution-precipitation processes appear to have no severe consequences on reservoir and cap rock integrity or on the injection behaviour. This is also in line with the continuously recorded injection operation parameter. These do not point to any changes in reservoir injectivity.|

  5. Characterization of PM2.5 aerosols dominated by local pollution and Asian dust observed at an urban site in Korea during aerosol characterization experiments (ACE)--Asia Project.

    PubMed

    Park, Seung Shik; Kim, Young J; Cho, Sung Yong; Kim, Seung Jai

    2007-04-01

    Daily fine particulate matter (PM2.5) samples were collected at Gwangju, Korea, during the Aerosol Characterization Experiments (ACE)-Asia Project to determine the chemical properties of PM2.5 originating from local pollution and Asian dust (AD) storms. During the study period, two significant events occurred on April 10-13 and 24-25, 2001, and a minor event occurred on April 19, 2001. Based on air mass transport pathways identified by back-trajectory calculation, the PM2.5 dataset was classified into three types of aerosol populations: local pollution and two AD aerosol types. The two AD types were transported along different pathways. One originated from Gobi desert area in Mongolia, passing through Hunshandake desert in Northern Inner Mongolia, urban and polluted regions of China (AD1), and the other originated in sandy deserts located in the Northeast Inner Mongolia Plateau and then flowed southward through the Korean peninsula (AD2). During the AD2 event, a smoke plume that originated in North Korea was transported to our study site. Mass balance closures show that crustal materials were the most significant species during both AD events, contributing -48% to the PM2.5 mass; sulfate aerosols (19.1%) and organic matter (OM; 24.6%) were the second greatest contributors during the AD1 and AD2 periods, respectively, indicating that aerosol properties were dependent on the transport pathway. The sulfate concentration constituted only 6.4% (4.5 microg/m3) of the AD2 PM2.5 mass. OM was the major chemical species in the local pollution-dominated PM2.5 aerosols, accounting for 28.7% of the measured PM2.5 mass, followed by sulfate (21.4%), nitrate (15%), ammonium (12.8%), elemental carbon (8.9%), and crustal material (6.5%). Together with substantial enhancement of the crustal elements (Mg, Al, K, Ca, Sc, Ti, Mn, Fe, Sr, Zr, Ba, and Ce), higher concentrations of pollution elements (S, V, Ni, Zn, As, Cd, and Pb) were observed during AD1 and AD2 than during the local

  6. An Advanced Organometallic Lab Experiment with Biological Implications: Synthesis and Characterization of Fe[subscript 2](µ-S[subscript 2])(C0)[subscript 6

    ERIC Educational Resources Information Center

    Barrett, Jacob; Spentzos, Ariana; Works, Carmen

    2015-01-01

    The organometallic complex Fe[subscript 2](µ-S[subscript 2])(CO)[subscript 6] has interesting biological implications. The concepts of bio-organometallic chemistry are rarely discussed at the undergraduate level, but this experiment can start such a conversation and, in addition, teach valuable synthetic techniques. The lab experiment takes a…

  7. West Texas array experiment: Noise and source characterization of short-range infrasound and acoustic signals, along with lab and field evaluation of Intermountain Laboratories infrasound microphones

    NASA Astrophysics Data System (ADS)

    Fisher, Aileen

    The term infrasound describes atmospheric sound waves with frequencies below 20 Hz, while acoustics are classified within the audible range of 20 Hz to 20 kHz. Infrasound and acoustic monitoring in the scientific community is hampered by low signal-to-noise ratios and a limited number of studies on regional and short-range noise and source characterization. The JASON Report (2005) suggests the infrasound community focus on more broad-frequency, observational studies within a tactical distance of 10 km. In keeping with that recommendation, this paper presents a study of regional and short-range atmospheric acoustic and infrasonic noise characterization, at a desert site in West Texas, covering a broad frequency range of 0.2 to 100 Hz. To spatially sample the band, a large number of infrasound gauges was needed. A laboratory instrument analysis is presented of the set of low-cost infrasound sensors used in this study, manufactured by Inter-Mountain Laboratories (IML). Analysis includes spectra, transfer functions and coherences to assess the stability and range of the gauges, and complements additional instrument testing by Sandia National Laboratories. The IMLs documented here have been found reliably coherent from 0.1 to 7 Hz without instrument correction. Corrections were built using corresponding time series from the commercially available and more expensive Chaparral infrasound gauge, so that the corrected IML outputs were able to closely mimic the Chaparral output. Arrays of gauges are needed for atmospheric sound signal processing. Our West Texas experiment consisted of a 1.5 km aperture, 23-gauge infrasound/acoustic array of IMLs, with a compact, 12 m diameter grid-array of rented IMLs at the center. To optimize signal recording, signal-to-noise ratio needs to be quantified with respect to both frequency band and coherence length. The higher-frequency grid array consisted of 25 microphones arranged in a five by five pattern with 3 meter spacing, without

  8. Characterization of a Solid Oxide Fuel Cell Gas Turbine Hybrid System Based on a Factorial Design of Experiments Using Hardware Simulation

    SciTech Connect

    Restrepo, Bernardo; Banta, Larry E.; Tucker, David

    2012-10-01

    A full factorial experimental design and a replicated fractional factorial design were carried out using the Hybrid Performance (HyPer) project facility installed at the National Energy Technology Laboratory (NETL), U.S. Department of Energy to simulate gasifer/fuel cell/turbine hybrid power systems. The HyPer facility uses hardware in the loop (HIL) technology that couples a modified recuperated gas turbine cycle with hardware driven by a solid oxide fuel cell model. A 34 full factorial design (FFD) was selected to study the effects of four factors: cold-air, hot-air, bleed-air bypass valves, and the electric load on different parameters such as cathode and turbine inlet temperatures, pressure and mass flow. The results obtained, compared with former results where the experiments were made using one-factor-at-a-time (OFAT), show that no strong interactions between the factors are present in the different parameters of the system. This work also presents a fractional factorial design (ffd) 34-2 in order to analyze replication of the experiments. In addition, a new envelope is described based on the results of the design of experiments (DoE), compared with OFAT experiments, and analyzed in an off-design integrated fuel cell/gas turbine framework. This paper describes the methodology, strategy, and results of these experiments that bring new knowledge concerning the operating state space for this kind of power generation system.

  9. Unconfined compression experiments on Topopah Spring Member tuff at 22{degrees}C and a strain rate of 10{sup {minus}9} s{sup {minus}1}: Data report; Yucca Mountain Site Characterization Project

    SciTech Connect

    Martin, R.J. III; Boyd, P.J.; Noel, J.S.; Price, R.H.

    1993-08-01

    Experiment results are presented for unconfined compressive strength and elastic moduli of tuffaceous rocks from Busted Butte near Yucca Mountain, Nevada. The data have been compiled for the Yucca Mountain Site Characterization Project Site and Engineering Properties Data Base. Experiments were conducted on water-saturated specimens of the potential nuclear waste repository horizon Topopah Spring Member tuff (thermal/mechanical unit TSw2). The influence of strain rate on mechanical properties of the tuff was examined by loading six specimens in uniaxial compression at a strain rate of 10{sup {minus}9} s{sup {minus}1}. The experiments performed under ambient pressure and temperature conditions and conformed to Technical Procedure 91, titled ``Unconfined Compression Experiments at 22{degrees}C and a Strain Rate of 10{sup {minus}9} s{sup {minus}1}.`` The mean and standard deviation values of ultimate strength, Young`s modulus and Poisson`s ratio determined from these experiments are 85.4{plus_minus}21.7 MPa, 33.9{plus_minus}4.6 GPa, and 0.09{plus_minus}0.07, respectively.

  10. Identification and Molecular Characterization of Two Acetylcholinesterases from the Salmon Louse, Lepeophtheirus salmonis

    PubMed Central

    Kaur, Kiranpreet; Bakke, Marit Jørgensen; Nilsen, Frank; Horsberg, Tor Einar

    2015-01-01

    Acetylcholinesterase (AChE) is an important enzyme in cholinergic synapses. Most arthropods have two genes (ace1 and ace2), but only one encodes the predominant synaptic AChE, the main target for organophosphates. Resistance towards organophosphates is widespread in the marine arthropod Lepeophtheirus salmonis. To understand this trait, it is essential to characterize the gene(s) coding for AChE(s). The full length cDNA sequences encoding two AChEs in L. salmonis were molecularly characterized in this study. The two ace genes were highly similar (83.5% similarity at protein level). Alignment to the L. salmonis genome revealed that both genes were located close to each other (separated by just 26.4 kbp on the L. salmonis genome), resulting from a recent gene duplication. Both proteins had all the typical features of functional AChE and clustered together with AChE-type 1 proteins in other species, an observation that has not been described in other arthropods. We therefore concluded the presence of two versions of ace1 gene in L. salmonis, named ace1a and ace1b. Ace1a was predominantly expressed in different developmental stages compared to ace1b and was possibly active in the cephalothorax, indicating that ace1a is more likely to play the major role in cholinergic synaptic transmission. The study is essential to understand the role of AChEs in resistance against organophosphates in L. salmonis. PMID:25938836

  11. Chemical characterization of the fine particle emissions from commercial aircraft engines during the Aircraft Particle Emissions eXperiment (APEX) 1 to 3

    EPA Science Inventory

    This paper addresses the need for detailed chemical information on the fine particulate matter (PM2.5) generated by commercial aviation engines. The exhaust plumes of nine engine models were sampled during the three test campaigns of the Aircraft Particle Emissions eXperiment (AP...

  12. Physical characterization of the fine particle emissions from commercial aircraft engines during the Aircraft Particle Emissions Experiment (APEX) 1 to 3

    EPA Science Inventory

    The f1me particulate matter (PM) emissions from nine commercial aircraft engine models were determined by plume sampling during the three field campaigns of the Aircraft Particle Emissions Experiment (APEX). Ground-based measurements were made primarily at 30 m behind the engine ...

  13. Generation, Isolation, and Characterization of a Stable Enol from Grignard Addition to a Bis-Ester: A Microscale Experiment for the Undergraduate Organic Chemistry Laboratory

    ERIC Educational Resources Information Center

    Nicaise, Olivier J. C.; Ostrom, Kyle F.; Dalke, Brent J.

    2005-01-01

    An experiment is described that introduces students to the concept of temperature-dependent stability of the tetrahedral intermediate in an acyl-transfer reaction. The process involves the determination of the structure of an alpha-ketoester and its corresponding remarkably stable enol ester to suggest a mechanism for the formation of the products.

  14. A conceptual framework for characterizing forest areas with high societal values: experiences from the Pacific Northwest of USA and Central Europe.

    PubMed

    Simončič, Tina; Spies, Thomas A; Deal, Robert L; Bončina, Andrej

    2015-07-01

    In recent decades, much work has been invested to describe forest allocations with high societal values. Yet, few comparative analyses have been conducted on their importance and differences across the regions of the globe. This paper introduces a conceptual framework to characterize forest priority areas defined as areas with identified higher importance of societal values in the context of multi-objective forest management. The six dimensions of the framework (designation objective, prioritization of objectives, governance, permanency, spatial scale, and management regime) characterize the general approach (integrative vs. segregative) to multi-objective forest management and explain the form and role of priority areas for providing forest services. The framework was applied in two case study regions--Pacific Northwest of USA (PNW) and Central Europe (CE). Differences between the regions exist in all dimensions. Late-successional and riparian reserves are specific to the PNW, while protection against natural hazards is specific to CE. In PNW, priority areas are mainly focused on public lands whereas in CE they include public and private lands. Priority areas in PNW are designated in a much larger spatial context and have longer time commitments. In CE, integration of management objectives on priority areas prevails, whereas in PNW priority areas tend to be designated for single objectives. In CE, greater tolerance of timber management within priority areas compared to PNW is allowed. Convergent trends in application of priority areas between the regions indicate mixing of segregation and integration approaches to forest management. PMID:25894271

  15. A Conceptual Framework for Characterizing Forest Areas with High Societal Values: Experiences from the Pacific Northwest of USA and Central Europe

    NASA Astrophysics Data System (ADS)

    Simončič, Tina; Spies, Thomas A.; Deal, Robert L.; Bončina, Andrej

    2015-07-01

    In recent decades, much work has been invested to describe forest allocations with high societal values. Yet, few comparative analyses have been conducted on their importance and differences across the regions of the globe. This paper introduces a conceptual framework to characterize forest priority areas defined as areas with identified higher importance of societal values in the context of multi-objective forest management. The six dimensions of the framework (designation objective, prioritization of objectives, governance, permanency, spatial scale, and management regime) characterize the general approach (integrative vs. segregative) to multi-objective forest management and explain the form and role of priority areas for providing forest services. The framework was applied in two case study regions—Pacific Northwest of USA (PNW) and Central Europe (CE). Differences between the regions exist in all dimensions. Late-successional and riparian reserves are specific to the PNW, while protection against natural hazards is specific to CE. In PNW, priority areas are mainly focused on public lands whereas in CE they include public and private lands. Priority areas in PNW are designated in a much larger spatial context and have longer time commitments. In CE, integration of management objectives on priority areas prevails, whereas in PNW priority areas tend to be designated for single objectives. In CE, greater tolerance of timber management within priority areas compared to PNW is allowed. Convergent trends in application of priority areas between the regions indicate mixing of segregation and integration approaches to forest management.

  16. Identification and characterization of stressed degradation products of metoprolol using LC/Q-TOF-ESI-MS/MS and MS(n) experiments.

    PubMed

    Borkar, Roshan M; Raju, B; Srinivas, R; Patel, Prashant; Shetty, Satheesh Kumar

    2012-06-01

    A rapid, specific and reliable isocratic high-performance liquid chromatography combined with quadrupole time-of-flight electrospray ionization tandem mass spectrometry (LC/Q-TOF-ESI-MS/MS) method has been developed and validated for the identification and characterization of stressed degradation products of metoprolol. Metoprolol, an anti-hypertensive drug, was subjected to hydrolysis (acidic, alkaline and neutral), oxidation, photolysis and thermal stress, as per ICH-specified conditions. The drug showed extensive degradation under oxidative and hydrolysis (acid and base) stress conditions. However, it was stable to thermal, neutral and photolysis stress conditions. A total of 14 degradation products were observed and the chromatographic separation of the drug and its degradation products was achieved on a C(18) column (4.6 × 250 mm, 5 µm). To characterize degradation products, initially the mass spectral fragmentation pathway of the drug was established with the help of MS/MS, MS(n) and accurate mass measurements. Similarly, fragmentation pattern and accurate masses of the degradation products were established by subjecting them to LC-MS/QTOF analysis. Structure elucidation of degradation products was achieved by comparing their fragmentation pattern with that of the drug. The degradation products DP(2) (m/z 153) and DP(14) (m/z 236) were matched with impurity B, listed in European Pharmacopoeia and British Pharmacopoeia, and impurity I, respectively. The LC-MS method was validated with respect to specificity, linearity, accuracy and precision. PMID:21989963

  17. Shuttle Mission STS-50: Orbital Processing of High-Quality CdTe Compound Semiconductors Experiment: Final Flight Sample Characterization Report

    NASA Technical Reports Server (NTRS)

    Larson, David J.; Casagrande, Luis G.; DiMarzio, Don; Alexander, J. Iwan D.; Carlson, Fred; Lee, Taipo; Dudley, Michael; Raghathamachar, Balaji

    1998-01-01

    The Orbital Processing of High-Quality Doped and Alloyed CdTe Compound Semiconductors program was initiated to investigate, quantitatively, the influences of gravitationally dependent phenomena on the growth and quality of bulk compound semiconductors. The objective was to improve crystal quality (both structural and compositional) and to better understand and control the variables within the crystal growth production process. The empirical effort entailed the development of a terrestrial (one-g) experiment baseline for quantitative comparison with microgravity (mu-g) results. This effort was supported by the development of high-fidelity process models of heat transfer, fluid flow and solute redistribution, and thermo-mechanical stress occurring in the furnace, safety cartridge, ampoule, and crystal throughout the melting, seeding, crystal growth, and post-solidification processing. In addition, the sensitivity of the orbital experiments was analyzed with respect to the residual microgravity (mu-g) environment, both steady state and g-jitter. CdZnTe crystals were grown in one-g and in mu-g. Crystals processed terrestrially were grown at the NASA Ground Control Experiments Laboratory (GCEL) and at Grumman Aerospace Corporation (now Northrop Grumman Corporation). Two mu-g crystals were grown in the Crystal Growth Furnace (CGF) during the First United States Microgravity Laboratory Mission (USML-1), STS-50, June 24 - July 9, 1992.

  18. Characterizing the Velocity Profile of a Swirling Gas Experiment by Particle Imaging Velocimetry to Study Angular Momentum Transport in Accretion Disks

    NASA Astrophysics Data System (ADS)

    Greess, Samuel; Ji, Hantao; Merino, Enrique; Berrios, William

    2013-10-01

    The method by which angular momentum transfers between different sections of accretion disks is a matter of ongoing debate. One suggested answer is Magnetorotational instability (MRI), which would facilitate this transfer through the magnetic interactions between particles at different distances from the center of the disk. While ongoing experiments with MRI have focused on the use of liquid metals to test the effects of magnetic fields, we are developing a swirling gas experiment to study effects beyond incompressible hydrodynamics, including compressible gas dynamics and plasma effects when gas is ionized. A second-generation prototype swirling gas experiment has been built to test the principle and to establish favorable rotation profiles using a chamber of swirling fog to simulate the formation and movement of accretion disks about some gravitational center. The paths of the visible fog particles can then be analyzed with Particle Imaging Velocimetry (PIV) techniques; these velocity measurements can then be organized by a Python program. Anticipated results include a radial profile of velocities at different times during the gas injection process, as well as further refinement of the fog chamber design to improve the accuracy in controlling the profile.

  19. The design and characterization of toroidal-shaped Nova hohlraums that simulate National Ignition Facility plasma conditions for plasma instability experiments

    SciTech Connect

    Wilde, B.H.; Fernandez, J.C.; Hsing, W.W.; Cobble, J.A.; Delamater, N.D.; Krauser, W.J.; Lindman, E.L.; Failor, B.H.

    1995-07-01

    Special Nova hohlraums have been designed to simulate the plasma conditions calculated for various NIF hohlraum point designs. These hohlraums attempt to maximize the laser pathlength for parametric instability measurements. A toroidal-shaped hohlraum with a diameter of 3200 microns and a length of 1600 microns allows a laser pathlength of about 2 mm. Filling the hohlraum with 1 atmosphere of neopentane gas gives an electron temperature of 3 keV and electron density near 0.1 of critical. Detailed LASNEX calculations for these hohlraums and comparisons to the NIF point design will be presented. Comparisons between data and calculations that characterize the plasma conditions (electron, radiation, and ion temperatures, electron density, etc) in these Nova hohlraums will also be shown.

  20. Characterization of large area, thick, and segmented silicon detector for electron and proton detection from neutron beta decay experiments in the cold and ultracold energies

    NASA Astrophysics Data System (ADS)

    Salas Bacci, Americo; McGaughey, Patrick; Baessler, Stefan; Broussard, Leah; Makela, Mark; Mirabal, Jacqueline; Pattie, Robert; Pocanic, Dinko; Hoedl, Seth; Sjue, Sky; Penttila, Seppo; Hasan, Syed; Wilburn, Scott; Young, Albert; Zeck, Bryan; Wang, Zhehui

    2012-10-01

    The ``Nab'' and ``UCNB'' collaborations have proposed to measure the correlation parameters in neutron β-decay at Oak Ridge and Los Alamos National Laboratory, using a novel detector design and electromagnetic spectrometers. Two large area, thick, hexagonal-segmented Silicon detectors containing 128 pixels per detector are going to be used to detect the electron and proton from neutron decay. Both Silicon detectors are connected by magnetic field lines of few Tesla field strength, and set on an electrostatic potential, such that protons can be accelerated up to 30 keV in order to be detected. We report the characterization, operation, proton detection from 15 to 30 keV, total pulse height defect, computation of atomic scattering defect, recombination defect, and evaluation of dead layer for these large area and thick Silicon detectors.

  1. Microstructural Characterization of the U-9.1Mo Fuel/AA6061 Cladding Interface in Friction-Bonded Monolithic Fuel Plates Irradiated in the RERTR-6 Experiment

    SciTech Connect

    Keiser, Dennis D.; Jue, Jan-Fong; Miller, Brandon; Gan, Jian; Robinson, Adam; Medvedev, Pavel; Madden, James; Wachs, Dan; Clark, Curtis; Meyer, Mitch

    2015-09-01

    Low-enrichment (U-235 < 20%) U-Mo monolithic fuel is being developed for use in research and test reactors. The earliest design for this fuel that was investigated via reactor testing was comprised of a nominally U-10Mo fuel foil encased in AA6061 (Al-6061) cladding. For a fuel design to be deemed adequate for final use in a reactor, it must maintain dimensional stability and retain fission products throughout irradiation, which means that there must be good integrity at the fuel foil/cladding interface. To investigate the nature of the fuel/cladding interface for this fuel type after irradiation, fuel plates that were tested in INL's Advanced Test Reactor (ATR) were subsequently characterized using optical metallography, scanning electron microscopy, and transmission electron microscopy. Results of this characterization showed that the fuel/cladding interaction layers present at the U-Mo fuel/AA6061 cladding interface after fabrication became amorphous during irradiation. Up to two main interaction layers, based on composition, could be found at the fuel/cladding interface, depending on location. After irradiation, an Al-rich layer contained very few fission gas bubbles, but did exhibit Xe enrichment near the AA6061 cladding interface. Another layer, which contained more Si, had more observable fission gas bubbles. Adjacent to the AA6061 cladding were Mg-rich precipitates, which was in close proximity to the region where Xe is observed to be enriched. In samples produced using a focused ion beam at the interaction zone/AA6061 cladding interface were possible indications of porosity/debonding, which suggested that the interface in this location is relatively weak.

  2. Microstructural Characterization of the U-9.1Mo Fuel/AA6061 Cladding Interface in Friction-Bonded Monolithic Fuel Plates Irradiated in the RERTR-6 Experiment

    NASA Astrophysics Data System (ADS)

    Keiser, Dennis D.; Jue, Jan-Fong; Miller, Brandon; Gan, Jian; Robinson, Adam; Medvedev, Pavel; Madden, James; Wachs, Dan; Clark, Curtis; Meyer, Mitch

    2015-09-01

    Low-enrichment (235U < 20 pct) U-Mo monolithic fuel is being developed for use in research and test reactors. The earliest design for this fuel that was investigated via reactor testing consisted of a nominally U-10Mo fuel foil encased in AA6061 (Al-6061) cladding. For a fuel design to be deemed adequate for final use in a reactor, it must maintain dimensional stability and retain fission products throughout irradiation, which means that there must be good integrity at the fuel foil/cladding interface. To investigate the nature of the fuel/cladding interface for this fuel type after irradiation, fuel plates were fabricated using a friction bonding process, tested in INL's advanced test reactor (ATR), and then subsequently characterized using optical metallography, scanning electron microscopy, and transmission electron microscopy. Results of this characterization showed that the fuel/cladding interaction layers present at the U-Mo fuel/AA6061 cladding interface after fabrication became amorphous during irradiation. Up to two main interaction layers, based on composition, could be found at the fuel/cladding interface, depending on location. After irradiation, an Al-rich layer contained very few fission gas bubbles, but did exhibit Xe enrichment near the AA6061 cladding interface. Another layer, which contained more Si, had more observable fission gas bubbles. In the samples produced using a focused ion beam at the interaction zone/AA6061 cladding interface, possible indications of porosity/debonding were found, which suggested that the interface in this location is relatively weak.

  3. Characterization of Anomalous Transport in Fractured Rock through the Imaging of Push-Pull Experiments Using Single-Hole Ground Penetrating Radar Reflection Data

    NASA Astrophysics Data System (ADS)

    Shakas, A.; Linde, N.; Baron, L.; Bour, O.; Bochet, O.; Le Borgne, T.

    2014-12-01

    Interpretation of push-pull data is limited by the absence of a clearly defined length scale, information on subsurface heterogeneity, and the assumption that ambient flow is negligible. We address these issues through a series of push-pull experiments with saline and deionized tracers at a granitic experimental research site in Ploemeur, France. The experiments involve different injection fractures, chaser volumes and resting times. Monitoring is performed in a neighboring borehole (~6m away) by single-hole ground penetrating radar (GPR), in both moving and fixed antenna settings. The GPR borehole is isolated from the tracer using a flexible-liner. We clearly image the dominant fractures in which tracer transport takes place and the spatial extent of the tracer plume at different times, which varies from a few meters to more than 10 meters away from the injection point. The differences between time-lapse images are subtle and consistent and in good agreement with the concentration data. We identify fractures in which the tracer is trapped for longer time periods and postulate that these fractures are partly responsible for the observed tailing behavior. By increasing the chasing volume, we see the tracer moving further away from the injection point and occupying different fractures, but our ability to image the tracer retrieval is decreased. By varying the resting time of a given experiment we identify regions of density-driven flow, heterogeneous advection, and ambient flow. Deionized water minimizes density effects, but the response is more quickly attenuated compared with saline tracers. The fixed antenna acquisitions provide data of unprecedented quality that display temporal dynamics in agreement with the concentration data down to very low concentrations. This data set offers very exciting perspectives for coupled hydrogeophysical inversion aimed at understanding anomalous transport in fractured rock formations.

  4. Experiments with Aspirin.

    ERIC Educational Resources Information Center

    Borer, Londa L.; Barry, Edward

    2000-01-01

    Presents a series of experiments that can be used to demonstrate how aspirin can be synthesized and characterized, how the hydrolysis of aspirin can be used as an introduction to kinetics, and how coordination chemistry (chelation) can be introduced by preparing and characterizing the copper complexes of aspirin and salicylic acid. (Contains over…

  5. Characterizing a fast-response, low-afterglow liquid scintillator for neutron time-of-flight diagnostics in fast ignition experiments

    SciTech Connect

    Abe, Y. Hosoda, H.; Arikawa, Y.; Nagai, T.; Kojima, S.; Sakata, S.; Inoue, H.; Iwasa, Y.; Iwano, K.; Yamanoi, K.; Fujioka, S.; Nakai, M.; Sarukura, N.; Shiraga, H.; Norimatsu, T.; Azechi, H.

    2014-11-15

    The characteristics of oxygen-enriched liquid scintillators with very low afterglow are investigated and optimized for application to a single-hit neutron spectrometer for fast ignition experiments. It is found that 1,2,4-trimethylbenzene has better characteristics as a liquid scintillator solvent than the conventional solvent, p-xylene. In addition, a benzophenon-doped BBQ liquid scintillator is shown to demonstrate very rapid time response, and therefore has potential for further use in neutron diagnostics with fast time resolution.

  6. Calculation of experiment uncertainty in laboratory determination of several geoengineering properties of tuffs from Yucca Mountain, Nevada; Yucca Mountain Site Characterization Project

    SciTech Connect

    Nimick, F.B.; Schwartz, B.M.; Price, R.H.

    1991-11-01

    A method for estimating the precision and accuracy of measured parameters is described. Examples of application of the estimating method are presented for density, porosity, compressive strength, Young`s modulus, Poisson`s ratio, and thermal expansion. With the exception of the coefficient of linear thermal expansion, all laboratory data for these properties for tuff samples appear to have reasonable experiment uncertainties. Uncertainties in the thermal-expansion coefficients may be as high as 39% of the values, although most uncertainties for the coefficients are probably {le}2%. 4 refs., 1 fig., 15 tabs.

  7. Transport Experiments

    NASA Technical Reports Server (NTRS)

    Hall, Timothy M.; Wuebbles, Donald J.; Boering, Kristie A.; Eckman, Richard S.; Lerner, Jean; Plumb, R. Alan; Rind, David H.; Rinsland, Curtis P.; Waugh, Darryn W.; Wei, Chu-Feng

    1999-01-01

    MM II defined a series of experiments to better understand and characterize model transport and to assess the realism of this transport by comparison to observations. Measurements from aircraft, balloon, and satellite, not yet available at the time of MM I [Prather and Remsberg, 1993], provide new and stringent constraints on model transport, and address the limits of our transport modeling abilities. Simulations of the idealized tracers the age spectrum, and propagating boundary conditions, and conserved HSCT-like emissions probe the relative roles of different model transport mechanisms, while simulations of SF6 and C02 make the connection to observations. Some of the tracers are related, and transport diagnostics such as the mean age can be derived from more than one of the experiments for comparison to observations. The goals of the transport experiments are: (1) To isolate the effects of transport in models from other processes; (2) To assess model transport for realistic tracers (such as SF6 and C02) for comparison to observations; (3) To use certain idealized tracers to isolate model mechanisms and relationships to atmospheric chemical perturbations; (4) To identify strengths and weaknesses of the treatment of transport processes in the models; (5) To relate evaluated shortcomings to aspects of model formulation. The following section are included:Executive Summary, Introduction, Age Spectrum, Observation, Tropical Transport in Models, Global Mean Age in Models, Source-Transport Covariance, HSCT "ANOY" Tracer Distributions, and Summary and Conclusions.

  8. Geochemical and mineralogical characterization of the Arbuckle aquifer with laboratory flow cell experiments under supercritical conditions: Implications for CO2 sequestration

    NASA Astrophysics Data System (ADS)

    Barker, R. L.; Watney, W. L.; Bhattacharya, S.; Strazisar, B. R.; Kelly, L.; Ford, S. K.; Datta, S.

    2011-12-01

    The deep saline aquifer in south-central Kansas has been proposed as a potential site for geologic storage for CO2. Two wells (KGS 1-32 and 1-28) have been drilled to the basement to provide data for a site specific determination of the storage potential of the Arbuckle. The entirety of the Arbuckle (~4100-5100 ft) was cored to provide rock samples for description and flow cell experiments. Initial examination of the formation rocks show heterogeneity throughout the core samples that makes evident the need for careful examination of the formation to ensure accurate geochemical modeling in determining the storage capacity and extent of mineralization within injection rocks. The dominant mineralogy in the proposed CO2 injection zone is dolomitic limestone with sporadic large cherty nodules. Presence of extensive vugs and micro fractures are common at some depths. Thin section and XRD data have provided the specific mineral assemblage of each core plug. Drill stem test water samples were collected from 8 depths throughout the aquifer to describe the changing chemistry of water with depth. Initial chemical analysis show a hyper saline brine (range~50,000 - 190,000 TDS) dominated by Cl, Na and Ca. Elemental ratios of Cl:Br, Na:Cl and Ca:Sr are what is expected of a typical saline aquifer system. The swabbed water from 4995 to 5020 ft gave a constant pH of 4.76 for the entire period of pumping and field results show high sulfate concentrations (>200 mg/L). Laboratory experiments carried out at the National Energy Technology Laboratory at formation temperatures and pressures using formation core plugs and collected brine to identify the major reaction that can be anticipated when supercritical CO2 is in place. Formation brine is injected into the core plugs and supercritical CO2 is added thereafter. The effluent is collected as a time series of 1, 3, 6, 12, 18, 24, 32, 48 and 72 hours and analyzed for major, trace elements and anions by ICP-OES and IC to see the chemical

  9. Airborne Tunable Laser Absorption Spectrometer (ATLAS) instrument characterization: Accuracy of the AASE (Airborne Arctic Stratospheric Expedition) and AAOE (Airborne Antarctic Ozone Experiment) nitrous oxide data sets

    SciTech Connect

    Loewenstein, M.; Podolske, J.R. ); Strahan, S.E. )

    1990-03-01

    ATLAS, the Airborne Tunable Laser Absorption Spectrometer, was used to measure nitrous oxide in the 1987 Airborne Antarctic Ozone Experiment (AAOE) and in the 1989 Airborne Arctic Stratospheric Expedition (AASE). After the AASE, a detailed study of the ATLAS characteristics was undertaken to quantify the error inherent in the in situ measurement of atmospheric N{sub 2}O. Using the latest calibration of the ATLAS (June 1989) and incorporating the recognized errors arising in the flight environment of ATLAS, the authors have established that for both the AASE and the AAOE most of the acquired N{sub 2}O data sets are accurate to {plus minus}10% (2 sigma). Data from two of the earlier AAOE flights had a larger uncertainty.

  10. Failure to upregulate Agrp and Orexin in response to activity based anorexia in weight loss vulnerable rats characterized by passive stress coping and prenatal stress experience.

    PubMed

    Boersma, Gretha J; Liang, Nu-Chu; Lee, Richard S; Albertz, Jennifer D; Kastelein, Anneke; Moody, Laura A; Aryal, Shivani; Moran, Timothy H; Tamashiro, Kellie L

    2016-05-01

    We hypothesize that anorexia nervosa (AN) poses a physiological stress. Therefore, the way an individual copes with stress may affect AN vulnerability. Since prenatal stress (PNS) exposure alters stress responsivity in offspring this may increase their risk of developing AN. We tested this hypothesis using the activity based anorexia (ABA) rat model in control and PNS rats that were characterized by either proactive or passive stress-coping behavior. We found that PNS passively coping rats ate less and lost more weight during the ABA paradigm. Exposure to ABA resulted in higher baseline corticosterone and lower insulin levels in all groups. However, leptin levels were only decreased in rats with a proactive stress-coping style. Similarly, ghrelin levels were increased only in proactively coping ABA rats. Neuropeptide Y (Npy) expression was increased and proopiomelanocortin (Pomc) expression was decreased in all rats exposed to ABA. In contrast, agouti-related peptide (Agrp) and orexin (Hctr) expression were increased in all but the PNS passively coping ABA rats. Furthermore, DNA methylation of the orexin gene was increased after ABA in proactive coping rats and not in passive coping rats. Overall our study suggests that passive PNS rats have innate impairments in leptin and ghrelin in responses to starvation combined with prenatal stress associated impairments in Agrp and orexin expression in response to starvation. These impairments may underlie decreased food intake and associated heightened body weight loss during ABA in the passively coping PNS rats. PMID:26907996

  11. Fast response resonance fluorescence CO measurements aboard the C-130: Instrument characterization and measurements made during North Atlantic Regional Experiment 1993

    NASA Astrophysics Data System (ADS)

    Gerbig, Christoph; Kley, Dieter; Volz-Thomas, Andreas; Kent, Joss; Dewey, Ken; McKenna, Danny S.

    1996-12-01

    The resonance fluorescence instrument for the measurement of atmospheric CO described by Volz and Kley [1985] was characterized in the laboratory and adapted for use on aircraft. A major finding was that the background signal is largely due to continuum resonance Raman scattering by molecular oxygen and thus cannot be reduced by better design. The instrument was deployed on the United Kingdom Meteorological Office (UKMO) C-130 Hercules during August 1993 and in subsequent missions. The instrument achieved a detection limit (3σ) of 5 ppb at a time resolution of 30 s. For a typical CO concentration of 100 ppb, the signal-to-noise ratio (1σ) was 15 for an integration time of 2 s, which was the minimum time resolution that could be obtained during the flights because of limited pump capacity. Data collected over the North Atlantic show distinct layers of CO above the atmospheric boundary layer (ABL) that are well correlated with enhanced NOy mixing ratios and indicate transport of pollution from the American continent. Such layers, albeit much less pronounced, were encountered in westerly flow in the midtroposphere west of the coast of Portugal. Fairly high mixing ratios were observed in the lower troposphere associated with transport from southern Europe.

  12. The know unknowns: Detailed simulations and low-order modeling to characterize facility-induced non-idealities in chemical-kinetics experiments

    NASA Astrophysics Data System (ADS)

    Ihme, Matthias

    2013-11-01

    Experimental investigations to study chemical-kinetics processes, reaction-rates or ignition properties are frequently accompanied by facility-induced non-idealities. Examples are turbulence and thermo-viscous boundary layers in rapid compression machines, temperature fluctuations and mixture inhomogeneities in flow-reactors, or shock-bifurcations and pressure drifts in shock-tubes. Although experimental investigations are carefully conducted to mitigate these effects, they are difficult to quantify experimentally. Simulations can assist in identifying these non-idealities and in guiding experimental instrumentation to improve measurement accuracies. This presentation discusses three different modeling approaches to characterize facility-effects in rapid compression machines, flow reactors, and shock-tubes. After providing an overview about these facilities and describing the underlying models, examples are presented to illustrate effects of turbulence, mixture-inhomogeneities, heat-losses, and thermal stratification on the ignition dynamics in these facilities. Diagnostics is developed to assess the sensitivity of the induction chemistry and to quantify reliable operating regimes that are not contaminated by these non-ideal processes.

  13. Characterization of silicon micro-strip sensors with a pulsed infra-red laser system for the CBM experiment at FAIR

    NASA Astrophysics Data System (ADS)

    Ghosh, P.

    2015-03-01

    The Compressed Baryonic Matter (CBM) experiment at FAIR is composed of 8 tracking stations consisting of 1292 double sided silicon micro-strip sensors. For the quality assurance of produced prototype sensors a laser test system (LTS) has been developed. The aim of the LTS is to scan sensors with a pulsed infra-red laser driven by step motor to determine the charge sharing in-between strips and to measure qualitative uniformity of the sensor response over the whole active area. The prototype sensors which are tested with the LTS so far have 256 strips with a pitch of 50 μm on each side. They are read-out using a self-triggering prototype read-out electronic ASIC called n-XYTER. The LTS is designed to measure sensor response in an automatized procedure at several thousand positions across the sensor with focused infra-red laser light (spot size ≈ 12 μm , wavelength = 1060 nm). The pulse with duration (≈ 10 ns) and power (≈ 5 mW) of the laser pulses is selected such, that the absorption of the laser light in the 300 μm thick silicon sensors produces a number of about 24000 electrons, which is similar to the charge created by minimum ionizing particles (MIP) in these sensors. Laser scans different prototype sensors is reported.

  14. Exploiting Satellite Remote-Sensing Data in Fine Particulate Matter Characterization for Serving the Environmental Public Health Tracking Network (EPHTN): The HELIX-Atlanta Experience and NPOESS Implications

    NASA Technical Reports Server (NTRS)

    Al-Hamdan, Mohammad Z.; Crosson, William L.; Limaye, Ashutosh S.; Rickman, Douglas L.; Quattrochi, Dale A.; Estes, Maurice G.; Qualters, Judith R.; Sinclair, Amber H.; Tolsma, Dennis D.; Adeniyi, Kafayat A.

    2008-01-01

    As part of the U.S. National Environmental Public Health Tracking Network (EPHTN), the National Center for Environmental Health (NCEH) at the U.S. Centers for Disease Control and Prevention (CDC) led a project in collaboration with the National Aeronautics and Space Administration (NASA) Marshall Space Flight Center (MSFC) called Health and Environment Linked for Information Exchange (HELIX-Atlanta). Under HELIX-Atlanta, pilot projects were conducted to develop methods to better characterize exposure; link health and environmental datasets; and analyze spatial/temporal relationships. This paper describes and demonstrates different techniques for surfacing daily environmental hazards data of particulate matter with aerodynamic diameter less than or equal to 2.5 micrometers (PM(sub 2.5) for the purpose of integrating respiratory health and environmental data for the CDC's pilot study of HELIX-Atlanta. It describes a methodology for estimating ground-level continuous PM(sub 2.5) concentrations using spatial surfacing techniques and leveraging NASA Moderate Resolution Imaging Spectrometer (MODIS) data to complement the U.S. Environmental Protection Agency (EPA) ground observation data. The study used measurements of ambient PM(sub 2.5) from the EPA database for the year 2003 as well as PM(sub 2.5) estimates derived from NASA's MODIS data. Hazard data have been processed to derive the surrogate exposure PM(sub 2.5) estimates. The paper has shown that merging MODIS remote sensing data with surface observations of PM(sub 2.5), may provide a more complete daily representation of PM(sub 2.5), than either data set alone would allow, and can reduce the errors in the PM(sub 2.5) estimated surfaces. Future work in this area should focus on combining MODIS column measurements with profile information provided by satellites like the National Polar-orbiting Operational Environmental Satellite System (NPOESS). The Visible Infrared Imager/Radiometer Suite (VIIRS) and the Aerosol

  15. Time-Independent and Time-Dependent Rheological Characterization of Dispersions with Varying Contents of Chickpea Flour and Gum Arabic Employing the Multiple Loop Experiments.

    PubMed

    J, Shanthilal; Bhattacharya, Suvendu

    2016-08-01

    The chickpea (Cicer arietinum) flour dispersions as the model system with different contents of flour (37% to 43%) and gum arabic (0% to 5%) were subjected to multiple loop experiments for simultaneous determination of the time-independent and time-dependent rheological characteristics. The Herschel-Bulkley model was suitable (0.993 ≤ r ≤ 0.999) to relate the time-independent characteristics linking shear stress and shear rate data for the individual upward and downward curves. The yield stress, consistency index, and apparent viscosity increased with the increasing flour and/or gum contents while flow behavior index (n) decreased. The yield stress generally decreased with the number of loops but n increased. In the individual loop tests, the n values for the decreasing shear stress/shear rate curves were always higher than corresponding increasing curves meaning a shift toward Newtonian characteristics. The time-independent properties (yield stress, apparent viscosity, consistency index, and n), the time-dependent characteristics like the area of the loop, and liquid characteristics like pourability and the nonoral sensory attributes (viscosity, spreadability, and tackiness) were individually predicted by artificial neural networks wherein the root mean square errors were between 3.6% and 17.2%. The sensory assessment indicated that the desirable parameters for a free-flowing and easily pourable spherical chickpea batter droplets occurred when the average pourability and spreadability values were 6.9 and 5.9, respectively. The normalized indices for these 2 parameters indicated that the batter having 40% flour and 2% gum contents was most suitable exhibiting a deviation of only 10% from the ideal sensory scores; these values were 40% and 0% to 3%, and 43% and 0%, respectively exhibiting up to 20% deviation. PMID:27331658

  16. 1999-2003 Shortwave Characterizations of Earth Radiation Budget Satellite (ERBS)/Earth Radiation Budget Experiment (ERBE) Broadband Active Cavity Radiometer Sensors

    NASA Technical Reports Server (NTRS)

    Lee, Robert B., III; Smith, George L.; Wong, Takmeng

    2008-01-01

    From October 1984 through May 2005, the NASA Earth Radiation Budget Satellite (ERBS/ )/Earth Radiation Budget Experiment (ERBE)ERBE nonscanning active cavity radiometers (ACR) were used to monitor long-term changes in the earth radiation budget components of the incoming total solar irradiance (TSI), earth-reflected TSI, and earth-emitted outgoing longwave radiation (OLR). From September1984 through September 1999, using on-board calibration systems, the ERBS/ERBE ACR sensor response changes, in gains and offsets, were determined from on-orbit calibration sources and from direct observations of the incoming TSI through calibration solar ports at measurement precision levels approaching 0.5 W/sq m , at satellite altitudes. On October 6, 1999, the onboard radiometer calibration system elevation drive failed. Thereafter, special spacecraft maneuvers were performed to observe cold space and the sun in order to define the post-September 1999 geometry of the radiometer measurements, and to determine the October 1999-September 2003 ERBS sensor response changes. Analyses of these special solar and cold space observations indicate that the radiometers were pointing approximately 16 degrees away from the spacecraft nadir and on the anti-solar side of the spacecraft. The special observations indicated that the radiometers responses were stable at precision levels approaching 0.5 W/sq m . In this paper, the measurement geometry determinations and the determinations of the radiometers gain and offset are presented, which will permit the accurate processing of the October 1999 through September 2003 ERBE data products at satellite and top-of-the-atmosphere altitudes.

  17. Preparation and Characterization of the Activated Carbon-Nylon Beads: Novel Material for In Situ Microbe Sampler and Microcosm Experiment in Groundwater Environment

    NASA Astrophysics Data System (ADS)

    Liu, J.; Liu, H.

    2015-12-01

    The organic pollution of groundwater is a widespread problem in the word. It is significant to study the microbial community especially related to organic contaminant biodegradation and their variation with groundwater environment parameters, so as to evaluate the biodegradability of the organic contaminants and then make a right decision for bioremediation. One of good ways for this study is to build a microcosm in groundwater containing target contaminant, where microbes especially relating to biodegradation will grow in the microcosm and be collected for analysis. This research aims to prepare a novel material for in situ microbe sampler and microcosm experiment in groundwater environment. The novel material, namely, the activated carbon-nylon (AC-N) beads, was prepared using activated carbon and nylon as main raw materials. The material consists of 3-4mm diameter spherical beads (Fig.1A and Fig.2 A) which have an internal surface area greater than 500 m2 g-1. FT-IR spectra (Fig.3) indicated the composition of activated carbon and nylon due to the variation of the peaks at the near 1627 cm-1and 1558 -1538 cm-1 before and after complex reaction. The equilibrium adsorption capacity of benzene on the beads was 16.76 mg/g at the initial concentration of 100 mg/L. The adsorption kinetics was found to follow the pseudo-second-order kinetic model (Fig.4). The mechanism of the adsorption process was determined from the intraparticle diffusion model. Camera and SEM images (Fig.1 B and Fig.2 A and B) showed that the beads had an open and channel pore structures, the microbes might enter into and grow up in the beads (Fig.1 C and Fig.2 C). All these results showed that the AC-N beads could form the in situ microcosm of organic pollutants and microbes, which provided a promising prospect for assessing the biodegradability of the organic pollutants by intrinsic microbes in the groundwater.

  18. An overview of the Ice Nuclei Research Unit Jungfraujoch/Cloud and Aerosol Characterization Experiment 2013 (INUIT-JFJ/CLACE-2013)

    NASA Astrophysics Data System (ADS)

    Schneider, Johannes

    2014-05-01

    Ice formation in mixed phase tropospheric clouds is an essential prerequisite for the formation of precipitation at mid-latitudes. Ice formation at temperatures warmer than -35°C is only possible via heterogeneous ice nucleation, but up to now the exact pathways of heterogeneous ice formation are not sufficiently well understood. The research unit INUIT (Ice NUcleation research unIT), funded by the Deutsche Forschungsgemeinschaft (DFG FOR 1525) has been established in 2012 with the objective to investigate heterogeneous ice nucleation by combination of laboratory studies, model calculation and field experiments. The main field campaign of the INUIT project (INUIT-JFJ) was conducted at the High Alpine Research Station Jungfraujoch (Swiss Alps, 3580 m asl) during January and February 2013, in collaboration with several international partners in the framework of CLACE2013. The instrumentation included a large set of aerosol chemical and physical analysis instruments (particle counters, particle sizers, particle mass spectrometers, cloud condensation nuclei counters, ice nucleus counters etc.), that were operated inside the Sphinx laboratory and sampled in mixed phase clouds through two ice selective inlets (Ice-CVI, ISI) as well as through a total aerosol inlet that was used for out-of-cloud aerosol measurements. Besides the on-line measurements, also samples for off-line analysis (ESEM, STXM) have been taken in and out of clouds. Furthermore, several cloud microphysics instruments were operated outside the Sphinx laboratory. First results indicate that a large fraction of ice residues sampled from mixed phase clouds contain organic material, but also mineral dust. Soot and lead were not found to be enriched in ice residues. The concentration of heterogeneous ice nuclei was found to be variable (ranging between < 1 and > 100 per liter) and to be strongly dependent on the operating conditions of the respective IN counter. The number size distribution of ice residues

  19. Characterization of experimental dynamos

    NASA Astrophysics Data System (ADS)

    Peffley, Nicholas L.; Goumilevski, Alexei G.; Cawthrone, A. B.; Lathrop, Daniel P.

    2000-07-01

    Laboratory models of geophysical magnetic field production require new experi-mental characterization methods. Self-generating liquid metal magnetic dynamos are explored using two new experiments. Kinematic dynamo studies lead us to charac-terize the magnetic field dynamics in terms of eigenvalues and eigenfrequencies of the induction equation. Observing the decay of magnetic field pulses indicates the real part of the leading eigenvalue of the induction equation, while a chirp magnetic field diagnoses the imaginary part of the eigenvalue. Finally, a single-frequency applied magnetic field characterizes the structure of the velocity field. These measurements provide a new means to characterize and measure the approach to self-generation. We present data from numerical simulations and laboratory experiments using these techniques.

  20. Characterization of Water Vapor Fluxes by the Raman Lidar System Basil and the Univeristy of Cologne Wind Lidar in the Frame of the HD(CP)2 Observational Prototype Experiment - Hope

    NASA Astrophysics Data System (ADS)

    Di Girolamo, Paolo; Summa, Donato; Stelitano, Dario; Cacciani, Marco; Scoccione, Andrea; Schween, Jan H.

    2016-06-01

    Measurements carried out by the Raman lidar system BASIL and the University of Cologne wind lidar are reported to demonstrate the capability of these instruments to characterize water vapour fluxes within the Convective Boundary Layer (CBL). In order to determine the water vapour flux vertical profiles, high resolution water vapour and vertical wind speed measurements, with a temporal resolution of 1 sec and a vertical resolution of 15-90, are considered. Measurements of water vapour flux profiles are based on the application of covariance approach to the water vapour mixing ratio and vertical wind speed time series. The algorithms are applied to a case study (IOP 11, 04 May 2013) from the HD(CP)2 Observational Prototype Experiment (HOPE), held in Central Germany in the spring 2013. For this case study, the water vapour flux profile is characterized by increasing values throughout the CBL with lager values (around 0.1 g/kg m/s) in the entrainment region. The noise errors are demonstrated to be small enough to allow the derivation of water vapour flux profiles with sufficient accuracy.

  1. Mechanisms of Host Receptor Adaptation by Severe Acute Respiratory Syndrome Coronavirus

    SciTech Connect

    Wu, Kailang; Peng, Guiqing; Wilken, Matthew; Geraghty, Robert J.; Li, Fang

    2012-12-10

    The severe acute respiratory syndrome coronavirus (SARS-CoV) from palm civets has twice evolved the capacity to infect humans by gaining binding affinity for human receptor angiotensin-converting enzyme 2 (ACE2). Numerous mutations have been identified in the receptor-binding domain (RBD) of different SARS-CoV strains isolated from humans or civets. Why these mutations were naturally selected or how SARS-CoV evolved to adapt to different host receptors has been poorly understood, presenting evolutionary and epidemic conundrums. In this study, we investigated the impact of these mutations on receptor recognition, an important determinant of SARS-CoV infection and pathogenesis. Using a combination of biochemical, functional, and crystallographic approaches, we elucidated the molecular and structural mechanisms of each of these naturally selected RBD mutations. These mutations either strengthen favorable interactions or reduce unfavorable interactions with two virus-binding hot spots on ACE2, and by doing so, they enhance viral interactions with either human (hACE2) or civet (cACE2) ACE2. Therefore, these mutations were viral adaptations to either hACE2 or cACE2. To corroborate the above analysis, we designed and characterized two optimized RBDs. The human-optimized RBD contains all of the hACE2-adapted residues (Phe-442, Phe-472, Asn-479, Asp-480, and Thr-487) and possesses exceptionally high affinity for hACE2 but relative low affinity for cACE2. The civet-optimized RBD contains all of the cACE2-adapted residues (Tyr-442, Pro-472, Arg-479, Gly-480, and Thr-487) and possesses exceptionally high affinity for cACE2 and also substantial affinity for hACE2. These results not only illustrate the detailed mechanisms of host receptor adaptation by SARS-CoV but also provide a molecular and structural basis for tracking future SARS-CoV evolution in animals.

  2. Characterization of plasma formation and outflow emission from different wire-based z-pinch experiments driven at the 350kA, 1kA/ns Llampudken pulsed power driver

    NASA Astrophysics Data System (ADS)

    Veloso, Felipe; Muñoz-Cordovez, Gonzalo; Valenzuela-Villaseca, Vicente; Vescovi, Milenko; Favre, Mario; Wyndham, Edmund

    2015-11-01

    We present results on tungsten and aluminium wire-based z-pinch plasma experiments driven by the 350kA, 1kA/ns Llampudken generator at P Universidad Catolica de Chile. Our experiments are concentrated in the formation and subsequent emission of plasma from two different configurations: conical arrays and modified cylindrical arrays using different wire diameters within the load. The former produce collimated jet-like outflows by the zippering effect at the axis of the conical array, whereas the latter produce emission of an unstructured dense plasma object by the temporal variations on the global magnetic field topology of the cylindrical array. We present measurements of the ablation process in both configurations and the main features of the outflows obtained, such as plasma densities and propagation velocities. It is found that an appropriate mass per unit length in the load is particularly important for producing outflows from modified cylindrical arrays, and that high pressure background gas embedding the load hampers the emission of plasma outflows in conical arrays. In addition, the analysis of the dimensionless parameters that characterize each outflow will be presented. This work has been funded by FONDECYT 11121621. G Munoz is funded by a doctorate scholarship awarded by CONICYT.

  3. Mobile communications satellite antenna flight experiment definition

    NASA Technical Reports Server (NTRS)

    Freeland, Robert E.

    1987-01-01

    Results of a NASA-sponsored study to determine the technical feasibility and cost of a Shuttle-based flight experiment specifically intended for the MSAT commercial user community are presented. The experiment will include demonstrations of technology in the areas of radio frequency, sensing and control, and structures. The results of the structural subsystem study summarized here include experiment objective and technical approach, experiment structural description, structure/environment interactions, structural characterization, thermal characterization, structural measurement system, and experiment functional description.

  4. Experimenting with Electric Trains

    ERIC Educational Resources Information Center

    Wick, D. P.; Ramsdell, M. W.

    2007-01-01

    A simple experiment can be performed to characterize the relationship between applied voltage and velocity (steady state and transient) for an electric toy train. The results can be used by teams of students to solve a series of challenges in which they attempt to predict the performance of a particular train. Some sample challenges might include…

  5. ROCSAT-1 telecommunication experiments

    NASA Technical Reports Server (NTRS)

    Chang, J. F.; Taur, R. R.; Chu, T. H.; Li, H. S.; Su, Y. T.; Kiang, Y. W.; Su, S. L.; Shih, M. P.; Lin, H. D.; Chung, C. D.

    1993-01-01

    This paper addresses a telecommunication payload project approved by the R.O.C. NSPO's ROCSAT-1 space program. This project will enable several innovative experiments via the low-earth-orbit satellite ROCSAT-1, including multipath fading channel characterization, ionospheric scintillation measurement, real-time voice communications, and CDMA data communications. A unified L/S-band transponder payload is proposed for conducting these experiments in an efficient way. The results of these experiments would provide the evolving mobile communication communities with fruitful information.

  6. Diagnostic and Prognostic Properties of Osteoprotegerin in Patients with Acute Dyspnoea: Observations from the Akershus Cardiac Examination (ACE) 2 Study

    PubMed Central

    Pervez, Mohammed Osman; Pedersen, Marit Holmefjord; Brynildsen, Jon; Høiseth, Arne Didrik; Hagve, Tor-Arne; Røsjø, Helge; Omland, Torbjørn

    2016-01-01

    Background Circulating osteoprotegerin (OPG) levels are increased in patients with chronic heart failure (HF). The diagnostic and prognostic merit of OPG measurement in patients admitted with acute dyspnoea is unknown. Objectives To evaluate the diagnostic and prognostic value of measuring OPG in patients admitted to hospital with acute dyspnoea. Methods OPG was analysed by ELISA in 308 patients admitted due to acute dyspnoea. Investigators blinded to OPG results adjudicated the diagnosis for the index hospitalization. Clinical outcomes were obtained from hospital records. Results In total, 139 patients (45%) were hospitalized with acute HF. OPG levels on hospital admission were higher in patients with acute HF vs. no acute HF, 7.8 (5.5–10.4) vs. 5.4 (3.8–7.2) pmol/L, p<0.001. The area under the receiver operator characteristic curve (ROC AUC) of OPG to discriminate between HF vs. non-HF was 0.695 [95% CI 0.636–0.754]. OPG did not provide incremental information to the ED physician’s prediction or N-terminal pro-B-type natriuretic peptide regarding the diagnosis of acute HF. OPG levels (log transformed) were associated with mortality in crude analysis (HR (95% CI) 1.87 (1.34 to 2.61), p<0.001), but this association was attenuated and no longer significant after including established cardiac biomarkers into the model. Conclusion In patients admitted to hospital with acute dyspnoea, OPG levels are higher in patients with acute HF than in those with dyspnoea from other causes. However, OPG does not provide incremental information beyond ED physician assessment for the diagnosis of acute HF or beyond clinical risk variables and established cardiac biomarkers concerning prognosis. PMID:27463973

  7. Psychology Experiments.

    ERIC Educational Resources Information Center

    McGraw, Ken; Tew, Mark D.; Williams, John E.

    2001-01-01

    A goal of the PsychExperiments project was to reduce the financial burden on psychology departments for hardware/software used in their laboratories. In its third year, the PsychExperiments site now hosts 39 experiments. Over 200 classrooms worldwide have signed up as official site users and there have been nearly 10,000 data sessions conducted.…

  8. Characterization of Nanopipettes.

    PubMed

    Perry, David; Momotenko, Dmitry; Lazenby, Robert A; Kang, Minkyung; Unwin, Patrick R

    2016-05-17

    Nanopipettes are widely used in electrochemical and analytical techniques as tools for sizing, sequencing, sensing, delivery, and imaging. For all of these applications, the response of a nanopipette is strongly affected by its geometry and surface chemistry. As the size of nanopipettes becomes smaller, precise geometric characterization is increasingly important, especially if nanopipette probes are to be used for quantitative studies and analysis. This contribution highlights the combination of data from voltage-scanning ion conductivity experiments, transmission electron microscopy and finite element method simulations to fully characterize nanopipette geometry and surface charge characteristics, with an accuracy not achievable using existing approaches. Indeed, it is shown that presently used methods for characterization can lead to highly erroneous information on nanopipettes. The new approach to characterization further facilitates high-level quantification of the behavior of nanopipettes in electrochemical systems, as demonstrated herein for a scanning ion conductance microscope setup. PMID:27108872

  9. Molecular characterization of two acetylcholinesterase genes from the oriental tobacco budworm, Helicoverpa assulta (Guenée).

    PubMed

    Lee, Dae-Weon; Kim, Sung-Su; Shin, Seung Won; Kim, Won Tae; Boo, Kyung Saeng

    2006-02-01

    Acetylcholinesterase (AChE) has been known to be the target of organophosphorous and carbamate insecticides. Only a single AChE, however, existed in insects and was involved in insecticide resistance, recently another AChE is reported in mosquitoes and aphids. We have cloned cDNAs encoding two ace genes, designated as Ha-ace1 and Ha-ace2 by a combined degenerate PCR and RACE strategy from adult heads of the oriental tobacco budworm, Helicoverpa assulta. The Ha-ace1 and Ha-ace2 genes encode 664 and 647 amino acids, respectively and have conserved motifs including a catalytic triad, a choline-binding site and an acyl pocket. Both Ha-AChEs were determined to be secretory proteins based on the existence of a signal peptide. The Ha-ace1 gene, the first reported ace1 in lepidopterans, belongs to the ace1 subfamily whereas the Ha-ace2 gene showed high similarity to those in the ace2 subfamily. Phylogenetic analysis showed that the Ha-ace1 gene was completely diverged from the Ha-ace2, suggesting that the Ha-ace genes are duplicated. Quantitative real time-PCR revealed that expression level of the Ha-ace1 gene was much higher than that of the Ha-ace2 in all body parts examined. The biochemical properties of purified proteins by affinity chromatography showed substrate specificity for acetylthiocholine iodide, and inhibitor specificity for BW284C51 and eserine and their peptide sequences partially identified by a MALDI-TOF mass spectrometer demonstrated that two Ha-AChEs were expressed in vivo. PMID:16352398

  10. Apparatus and method for characterizing ultrafast polarization varying optical pulses

    DOEpatents

    Smirl, Arthur; Trebino, Rick P.

    1999-08-10

    Practical techniques are described for characterizing ultrafast potentially ultraweak, ultrashort optical pulses. The techniques are particularly suited to the measurement of signals from nonlinear optical materials characterization experiments, whose signals are generally too weak for full characterization using conventional techniques.

  11. Characterizing synthetic gypsum

    SciTech Connect

    Henkels, P.J.; Gaynor, J.C.

    1996-10-01

    Each gypsum wallboard manufacturer has developed its own general guidelines for synthetic gypsum. The guidelines vary accordingly for each manufacturer and are often modified to suite a particular source and end use. In addition, the physical and chemical properties of synthetic gypsum are characterized by several proprietary and published test methods. Characterizing a synthetic gypsum and determining its acceptability is a time consuming process and can be confusing, particularly to those outside the gypsum wallboard industry. This paper describes some of the more important characteristics and practical aspects of synthetic gypsum usage based on USG`s extensive experience in wall board manufacture.

  12. Language Experience

    ERIC Educational Resources Information Center

    Bugh, Marylou

    1978-01-01

    When a child uses his words and his ideas in learning to read, he also assists in the normal integration of his personality. Starting with a method of language experience developed by Sylvia Ashton-Warner, the author, a reading consultant, describes a language experience-reading program which utilizes the student's own curiosity and interests. (RK)

  13. Simulated Experiments

    ERIC Educational Resources Information Center

    Snadden, R. B.; Runquist, O.

    1975-01-01

    Presents an experiment in which a programmable calculator is employed as a data generating system for simulated laboratory experiments. The example used as an illustration is a simulated conductimetric titration of an aqueous solution of HC1 with an aqueous solution of NaOH. (Author/EB)

  14. TRIO experiment

    SciTech Connect

    Clemmer, R.G.; Finn, P.A.; Malecha, R.F.; Misra, B.; Billone, M.C.; Bowers, D.L.; Fischer, A.K.; Greenwood, L.R.; Mattas, R.F.; Tam, S.W.

    1984-09-01

    The TRIO experiment is a test of in-situ tritium recovery and heat transfer performance of a miniaturized solid breeder blanket assembly. The assembly (capsule) was monitored for temperature and neutron flux profiles during irradiation and a sweep gas flowed through the capsule to an anaytical train wherein the amounts of tritium in its various chemical forms were determined. The capsule was designed to operate at different temperatures and sweep gas conditions. At the end of the experiment the amount of tritium retained in the solid was at a concentration of less than 0.1 wppM. More than 99.9% of tritium generated during the experiment was successfully recovered. The results of the experiment showed that the tritium inventories at the beginning and at the end of the experiment follow a relationship which appears to be characteristic of intragranular diffusion.

  15. Experience Industry

    ERIC Educational Resources Information Center

    Laslett, R. L.

    1978-01-01

    The author, a tertiary chemistry teacher, spent a period of six weeks in a research project with an industrial firm. Describes his experience, the benefits and insight he gained from such an exchange. (GA)

  16. Interpretive Experiments

    ERIC Educational Resources Information Center

    DeHaan, Frank, Ed.

    1977-01-01

    Describes an interpretative experiment involving the application of symmetry and temperature-dependent proton and fluorine nmr spectroscopy to the solution of structural and kinetic problems in coordination chemistry. (MLH)

  17. Experiment Databases

    NASA Astrophysics Data System (ADS)

    Vanschoren, Joaquin; Blockeel, Hendrik

    Next to running machine learning algorithms based on inductive queries, much can be learned by immediately querying the combined results of many prior studies. Indeed, all around the globe, thousands of machine learning experiments are being executed on a daily basis, generating a constant stream of empirical information on machine learning techniques. While the information contained in these experiments might have many uses beyond their original intent, results are typically described very concisely in papers and discarded afterwards. If we properly store and organize these results in central databases, they can be immediately reused for further analysis, thus boosting future research. In this chapter, we propose the use of experiment databases: databases designed to collect all the necessary details of these experiments, and to intelligently organize them in online repositories to enable fast and thorough analysis of a myriad of collected results. They constitute an additional, queriable source of empirical meta-data based on principled descriptions of algorithm executions, without reimplementing the algorithms in an inductive database. As such, they engender a very dynamic, collaborative approach to experimentation, in which experiments can be freely shared, linked together, and immediately reused by researchers all over the world. They can be set up for personal use, to share results within a lab or to create open, community-wide repositories. Here, we provide a high-level overview of their design, and use an existing experiment database to answer various interesting research questions about machine learning algorithms and to verify a number of recent studies.

  18. The Experiment

    NASA Astrophysics Data System (ADS)

    Mariana Nicoara, Floare

    2016-04-01

    My name is Nicoara Floarea and I am teacher at Secondary School Calatele and I teach students from preparatory class and the second grade . They are six-eight years old. In my activity, for introducing scientific concepts to my students, I use various and active methods or traditional methods including experiments. The experiment stimulates students' curiosity, their creativity, the understanding and knowledge taught accessibility. I propose you two such experiments: The life cycle of the plants (long-term experiment, with rigorous observation time):We use beans, wheat or other; They are grown in pots and on the cotton soaked with water,keeping under students' observation protecting them ( just soak them regularly) and we waiting the plants rise. For discussions and comments of plant embryo development we use the plants which rose on the cotton soaked with water plants at the end of the first week. Last school year we had in the pot climbing beans which in May made pods. They were not too great but our experiment was a success. The students could deduce that there will develop those big beans which after drying will be planted again. The influence of light on plants (average duration experiment with the necessary observation time): We use two pots in which plants are of the same type (two geraniums), one of them is situated so as to get direct sunlight and other plant we put in a closed box. Although we wet both plants after a week we see that the plant that benefited from sunlight has turned strain in direct sunlight, developing normally in return the plant out of the box I have yellowed leaves, photosynthesis does not She has occurred . Students will understand the vital role of the Sun in plants' life, both in the classroom and in nature. The experiment is a method of teaching students extremely pleasant, with a remarkable percentage of acquiring more knowledge.

  19. Experiment 2042

    SciTech Connect

    Dash, Zora V.; Dennis, Bert R.; Dreesen, Donald S.; Fehler, Michael C.; House, Leigh S.; Walter, Fritz; Zyvoloski, George A.

    1984-09-10

    Experiment 2042, an injection test in EE-3, was conducted from May 15, 1984 through May 19, 1984. During this four day test ~2 million gallons of water were injected with a maximum injection rate of ~10BPM at 6000 psi. It was planned as a pumping test of the lower zone of well EE-3 (the open hole region from 11,400 ft to 11,648 ft) to test the reservoir characteristics and fracture-seismic system first created during Experiment 2025. However early in the experiment it became apparent that there was some sort connection between the lower zone and the upper "low pressure" zone in EE-3 (from the casing shoe at 10374 ft to about 10900 ft). Available information ruled out a packer failure or other direct connection between these zones so the experiment was continued as planned. Although not a major goal of the experiment, it was hoped that fractures would propagate from EE-3 to EE-2, so hydraulic communication could be established between the two wells, however this did not occur.

  20. Neutrino Experiments

    SciTech Connect

    McKeown, R. D.

    2010-08-04

    Recent studies of neutrino oscillations have established the existence of finite neutrino masses and mixing between generations of neutrinos. The combined results from studies of atmospheric neutrinos, solar neutrinos, reactor antineutrinos and neutrinos produced at accelerators paint an intriguing picture that clearly requires modification of the standard model of particle physics. These results also provide clear motivation for future neutrino oscillation experiments as well as searches for direct neutrino mass and nuclear double-beta decay. I will discuss the program of new neutrino oscillation experiments aimed at completing our knowledge of the neutrino mixing matrix.

  1. Cryogenic fluid management experiment

    NASA Technical Reports Server (NTRS)

    Eberhardt, R. N.; Bailey, W. J.; Fester, D. A.

    1981-01-01

    The cryogenic fluid management experiment (CFME), designed to characterize subcritical liquid hydrogen storage and expulsion in the low-q space environment, is discussed. The experiment utilizes a fine mesh screen fluid management device to accomplish gas-free liquid expulsion and a thermodynamic vent system to intercept heat leak and control tank pressure. The experiment design evolved from a single flight prototype to provision for a multimission (up to 7) capability. A detailed design of the CFME, a dynamic test article, and dedicated ground support equipment were generated. All materials and parts were identified, and components were selected and specifications prepared. Long lead titanium pressurant spheres and the flight tape recorder and ground reproduce unit were procured. Experiment integration with the shuttle orbiter, Spacelab, and KSC ground operations was coordinated with the appropriate NASA centers, and experiment interfaces were defined. Phase 1 ground and flight safety reviews were conducted. Costs were estimated for fabrication and assembly of the CFME, which will become the storage and supply tank for a cryogenic fluid management facility to investigate fluid management in space.

  2. Fat Characterization

    NASA Astrophysics Data System (ADS)

    Qian, Michael C.; Pike, Oscar A.

    Lipids in food are subjected to many chemical reactions during processing and storage. While some of these reactions are desirable, others are undesirable; so, efforts are made to minimize the reactions and their effects. The laboratory deals with the characterization of fats and oils with respect to composition, structure, and reactivity.

  3. Particle characterization for geothermal operations

    SciTech Connect

    Vetter, O.J.; Kandarpa, V.

    1981-01-06

    A detailed summary of an ongoing evaluation of existing particle measuring methodology with emphasis on (a) adapting of existing methods in geothermal operations and (b) further development of existing instrumentation for field use is presented. The various instruments and methods used and/or suggested for particle characterization are described in detail. Theoretical and practical aspects of particle characterizations are outlined. A plan for further laboratory and field experiments is outlined. The instrumentations to be selected after some additional lab and field tests will be used in the studies on (a) formation damage through particle invasion and (b) characterizing and monitoring of particle suspensions in geothermal operations.

  4. Soil experiment

    NASA Technical Reports Server (NTRS)

    Hutcheson, Linton; Butler, Todd; Smith, Mike; Cline, Charles; Scruggs, Steve; Zakhia, Nadim

    1987-01-01

    An experimental procedure was devised to investigate the effects of the lunar environment on the physical properties of simulated lunar soil. The test equipment and materials used consisted of a vacuum chamber, direct shear tester, static penetrometer, and fine grained basalt as the simulant. The vacuum chamber provides a medium for applying the environmental conditions to the soil experiment with the exception of gravity. The shear strength parameters are determined by the direct shear test. Strength parameters and the resistance of soil penetration by static loading will be investigated by the use of a static cone penetrometer. In order to conduct a soil experiment without going to the moon, a suitable lunar simulant must be selected. This simulant must resemble lunar soil in both composition and particle size. The soil that most resembles actual lunar soil is basalt. The soil parameters, as determined by the testing apparatus, will be used as design criteria for lunar soil engagement equipment.

  5. Analysis of NIF experiments

    NASA Astrophysics Data System (ADS)

    Wang, Yi-Ming

    2015-06-01

    A minimal energy implosion-scaling model was recently developed to characterize the physical properties of the hot spot in terms of the peak implosion energy. In this model, the hot spot energy, volume, pressure, mass and areal density at the stagnation time are uniquely determined by the peak implosion velocity, the equation of state and the adiabat of the pusher and the DT fuel (cold and hot) at the peak implosion time. In this work, we apply this model to a number of published low-foot and high-foot experiments performed at the National Ignition Facility. Our model analysis is in a good agreement with the experimental data when a high adiabat is assumed for both low and high foot experiments. Implications of the results are discussed. This work was performed under the auspices of the U.S. Department of Energy by the Los Alamos National Laboratory under Contract No. W-7405-ENG-36.

  6. Shooting Star Experiment

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The Shooting Star Experiment (SSE) is designed to develop and demonstrate the technology required to focus the sun's energy and use the energy for inexpensive space Propulsion Research. Pictured is an engineering model (Pathfinder III) of the Shooting Star Experiment (SSE). This model was used to test and characterize the motion and deformation of the structure caused by thermal effects. In this photograph, alignment targets are being placed on the engineering model so that a theodolite (alignment telescope) could be used to accurately measure the deformation and deflections of the engineering model under extreme conditions, such as the coldness of deep space and the hotness of the sun as well as vacuum. This thermal vacuum test was performed at the X-Ray Calibration Facility because of the size of the test article and the capabilities of the facility to simulate in-orbit conditions

  7. XMASS experiment

    NASA Astrophysics Data System (ADS)

    Abe, Ko

    2016-06-01

    XMASS is a single phase liquid xenon scintillator detector. The project is designed for multi purposes, dark matter, neutrinoless double beta decay and 7Be/pp solar neutrino. As the first step of project, XMASS-I detector with 832kg sensitive volume started operation from Dec. 2010. In this paper, recent obtained physics results from commissioning data, refurbishment of detector and future step of experiment are presented.

  8. SANE experiment

    SciTech Connect

    H. Baghdasaryan, SANE Collaboration

    2012-04-01

    The Spin Asymmetries of the Nucleon Experiment (SANE) is a measurement of parallel and near-perpendicular double spin asymmetries in an inclusive electron scattering. The main goal of the experiment was to measure A{sub {parallel}} and A{sub 80} and extract the spin asymmetries of the proton A{sub 1}{sup p}, A{sub 2}{sup p} and spin structure functions g{sub 1}{sup p} and g{sub 2}{sup p}. Using the Thomas Jefferson National Accelerator Facility's polarized electron beam and the University of Virginia's polarized frozen ammonia ({sup 14}NH{sub 3}) target in Hall C, the experiment ran in 2009, collecting data in a Q{sup 2} region from 2.5 to 6.5 GeV{sup 2} and between Bjorken x of 0.3 to 0.8. Particle detection was accomplished using the Big Electron Telescope Array (BETA), a novel non-magnetic detector. This talk will address the progress of the analysis designed to extract the proton spin asymmetries and structure functions. Preliminary results will be presented.

  9. Fat Characterization

    NASA Astrophysics Data System (ADS)

    O'Keefe, Sean F.; Pike, Oscar A.

    Methods for characterizing edible lipids, fats, and oils can be separated into two categories: those developed to analyze bulk oils and fats, and those focusing on analysis of foodstuffs and their lipid extracts. In evaluating foodstuffs, it is usually necessary to extract the lipids prior to analysis. In these cases, if sufficient quantities of lipids are available, methods developed for bulk fats and oils can be utilized.

  10. Pressure Core Characterization

    NASA Astrophysics Data System (ADS)

    Santamarina, J. C.

    2014-12-01

    Natural gas hydrates form under high fluid pressure and low temperature, and are found in permafrost, deep lakes or ocean sediments. Hydrate dissociation by depressurization and/or heating is accompanied by a multifold hydrate volume expansion and host sediments with low permeability experience massive destructuration. Proper characterization requires coring, recovery, manipulation and testing under P-T conditions within the stability field. Pressure core technology allows for the reliable characterization of hydrate bearing sediments within the stability field in order to address scientific and engineering needs, including the measurement of parameters used in hydro-thermo-mechanical analyses, and the monitoring of hydrate dissociation under controlled pressure, temperature, effective stress and chemical conditions. Inherent sampling effects remain and need to be addressed in test protocols and data interpretation. Pressure core technology has been deployed to study hydrate bearing sediments at several locations around the world. In addition to pressure core testing, a comprehensive characterization program should include sediment analysis, testing of reconstituted specimens (with and without synthetic hydrate), and in situ testing. Pressure core characterization technology can be used to study other gas-charged formations such as deep sea sediments, coal bed methane and gas shales.

  11. Characterization of shocked beryllium

    NASA Astrophysics Data System (ADS)

    Brown, E. N.; Cady, C. M.; Gray, G. T., III; Hull, L. M.; Cooley, J. H.; Bronkhorst, C. A.; Addessio, F. L.

    2014-05-01

    Explosively driven arrested beryllium experiments were performed with post mortem characterization to evaluate the failure behaviors. The test samples were encapsulated in an aluminum assembly that was large relative to the sample, and the assembly features both axial and radial momentum traps. The sample carrier was inserted from the explosively-loaded end and has features to lock the carrier to the surrounding cylinder using the induced plastic flow. Calculations with Lagrangian codes showed that the tensile stresses experienced by the Be sample were below the spall stress. Metallographic characterization of the arrested Be showed radial cracks present in the samples may have been caused by bending moments. Fractography showed the fractures propagated from the side of the sample closest to the explosives, the side with the highest tensile stress. There was evidence that the fractures may have propagated from the circumferential crack outward and downward radially.

  12. Identification of prolyl carboxypeptidase as an alternative enzyme for processing of renal angiotensin II using mass spectrometry

    PubMed Central

    Grobe, Nadja; Weir, Nathan M.; Leiva, Orly; Ong, Frank S.; Bernstein, Kenneth E.; Schmaier, Alvin H.; Morris, Mariana

    2013-01-01

    Angiotensin-converting enzyme 2 (ACE2) catalyzes conversion of ANG II to ANG-(1–7). The present study uses newly established proteomic approaches and genetic mouse models to examine the contribution of alternative renal peptidases to ACE2-independent formation of ANG-(1–7). In situ and in vitro mass spectrometric characterization showed that substrate concentration and pH control renal ANG II processing. At pH ≥6, ANG-(1–7) formation was significantly reduced in ACE2 knockout (KO) mice. However, at pH <6, formation of ANG-(1–7) in ACE2 KO mice was similar to that in wild-type (WT) mice, suggesting alternative peptidases for renal ANG II processing. Furthermore, the dual prolyl carboxypeptidase (PCP)-prolyl endopeptidase (PEP) inhibitor Z-prolyl-prolinal reduced ANG-(1–7) formation in ACE2 KO mice, while the ACE2 inhibitor MLN-4760 had no effect. Unlike the ACE2 KO mice, ANG-(1–7) formation from ANG II in PEP KO mice was not different from that in WT mice at any tested pH. However, at pH 5, this reaction was significantly reduced in kidneys and urine of PCP-depleted mice. In conclusion, results suggest that ACE2 metabolizes ANG II in the kidney at neutral and basic pH, while PCP catalyzes the same reaction at acidic pH. This is the first report demonstrating that renal ANG-(1–7) formation from ANG II is independent of ACE2. Elucidation of ACE2-independent ANG-(1–7) production pathways may have clinically important implications in patients with metabolic and renal disease. PMID:23392115

  13. Results from the HARP experiment

    NASA Astrophysics Data System (ADS)

    Radicioni, E.

    2008-07-01

    Hadron production is a key ingredient for precise prediction of atmospheric ν fluxes, characterization of accelerator ν beams, and quantification of π production and capture for ν-factory designs. HARP at the CERN PS was the first hadron production experiment designed on purpose to match all these requirements. We briefly describe here its most recent results.

  14. Chemistry Experiments

    NASA Technical Reports Server (NTRS)

    Brasseur, Guy; Remsberg, Ellis; Purcell, Patrick; Bhatt, Praful; Sage, Karen H.; Brown, Donald E.; Scott, Courtney J.; Ko, Malcolm K. W.; Tie, Xue-Xi; Huang, Theresa

    1999-01-01

    The purpose of the chemistry component of the model comparison is to assess to what extent differences in the formulation of chemical processes explain the variance between model results. Observed concentrations of chemical compounds are used to estimate to what degree the various models represent realistic situations. For readability, the materials for the chemistry experiment are reported in three separate sections. This section discussed the data used to evaluate the models in their simulation of the source gases and the Nitrogen compounds (NO(y)) and Chlorine compounds (Cl(y)) species.

  15. Characterizations of natural patterns

    NASA Astrophysics Data System (ADS)

    Gunaratne, Gemunu H.; Hoffman, David K.; Kouri, Donald J.

    1998-05-01

    Labyrinthine patterns are observed in systems as diverse as animal coats, slime mold colonies, fish scales, and cloud streets. However, even under well-controlled conditions, repetition of an experiment gives structures with vastly different details. A theoretical analysis of ``universal'' aspects of patterns requires a quantitative description that gives similar values for distinct configurations. We introduce a function to characterize the ``disorder'' of labyrinthine patterns that is the same for structures generated under identical control parameters. Furthermore, patterns with different visual characteristics are described by distinct functions.

  16. Voyager imaging experiment

    USGS Publications Warehouse

    Smith, B.A.; Briggs, G.A.; Danielson, G.E.; Cook, A.F., II; Davies, M.E.; Hunt, G.E.; Masursky, H.; Soderblom, L.A.; Owen, T.C.; Sagan, C.; Suomi, V.E.

    1977-01-01

    The overall objective of this experiment is exploratory reconnaissance of Jupiter, Saturn, their satellites, and Saturn's rings. Such reconnaissance, at resolutions and phase angles unobtainable from Earth, can be expected to provide much new data relevant to the atmospheric and/or surface properties of these bodies. The experiment also has the following specific objectives: Observe and characterize the global circulation of the atmospheres of Jupiter and Saturn; Determine the horizontal and vertical structure of the visible clouds and establish their relationship to the belted appearance and dynamical properties of the planetary atmospheres; Determine the vertical structure of high, optically-thin, scattering layers on Jupiter and Saturn; Determine the nature of anomalous features such as the Great Red Spot, South Equatorial Belt disturbances, etc.; Characterize the nature of the colored material in the clouds of Jupiter and Saturn, and identify the nature and sources of chromophores on Io and Titan; Perform comparative geologic studies of many satellites at less than 15-km resolution; Map and characterize the geologic structure of several satellites at high resolution (???1 km); Investigate the existence and nature of atmospheres on the satellites; Determine the mass, size, and shape of many of the satellites by direct measurement; Determine the direction of the spin axes and periods of rotation of several satellites, and establish coordinate systems for the larger satellites; Map the radial distribution of material in Saturn's rings at high resolution; Determine the optical scattering properties of the primaries, rings, and satellites at several wavelengths and phase angles; Search for novel physical phenomena, e.g., phenomena associated with the Io flux tube, meteors, aurorae, lightning, or satellite shadows. ?? 1977 D. Reidel Publishing Company.

  17. Transducer characterization

    SciTech Connect

    Cross, B. T.; Eoff, J. M.; Schuetz, L. J.; Cunningham, K. R.

    1980-07-02

    This report has been prepared specifically for ultrasonic transducer users within the Nondestructive Testing Evaluation (NDE) community of the weapons complex. The purpose of the report is to establish an initial set of uniform procedures for measuring and recording transducer performance data, and to establish a common foundation on which more comprehensive transducer performance evaluations may be added as future transducer performance criteria expands. Transducer parameters and the problems with measuring them are discussed and procedures for measuring transducer performance are recommended with special precautionary notes regarding critical aspects of each measurement. An important consideration regarding the recommended procedures is the cost of implementation. There are two distinct needs for transducer performance characterization in the complex. Production oriented users need a quick, reliable means to check a transducer to ascertain its suitability for continued service. Development groups and the Transducer Center need a comprehensive characterization means to collect adequate data to evaluate theoretical concepts or to build exact replacement transducers. The instrumentation, equipment, and procedures recommended for monitoring production transducers are utilitarian and provide only that information needed to determine transducer condition.

  18. AFIP-6 Characterization Summary Report

    SciTech Connect

    Dr. Dennis D. Keiser

    2011-12-01

    The AFIP-6 (ATR Full-size-plate In center flux trap Position) Characterization Summary Report outlines the fresh fuel characterization efforts performed during the AFIP-6 experiment. The AFIP-6 experiment was designed to evaluate the performance of monolithic uranium-molybdenum (U-Mo) fuels at a scale prototypic of Advanced Test Reactor (ATR) fuel plates (45-inches long). The AFIP-6 test was the first test with plates that were swaged into the rails of the assembly. This test served to examine the effects of a plate in a swaged condition with longer fuel zones (22.5-inches long), that were centered in the plate. AFIP-6 test plates employed a zirconium interlayer that was co-rolled with the fuel foil. Previous mini-plate and AFIP irradiation experiments performed in ATR have demonstrated the stable behavior of the interface between the U-Mo fuel and the zirconium interlayer.

  19. AMT experiment results

    NASA Technical Reports Server (NTRS)

    Abbe, Brian S.; Pinck, Deborah S.

    1995-01-01

    The Advanced Communications Technology Satellite (ACTS) Mobile Terminal (AMT) experiments have provided a terminal technology testbed for the evaluation of K- and Ka-band mobile satellite communications (satcom). Such a system could prove to be highly beneficial for many different commercial and government mobile satcom users. Combining ACTS' highly concentrated spotbeams with the smaller, higher-gain Ka-band antenna technology, results in a system design that can support a much higher throughput capacity than today's commercial configurations. To date, experiments in such diverse areas as emergency medical applications, enhanced Personal Communication Services (PCS), disaster recovery assistance, military applications, and general voice and data services have already been evaluated. Other applications that will be evaluated over the next year include telemedicine, ISDN, and television network return feed. Baseline AMT performance results will be presented, including Bit Error Rate (BER) curves and mobile propagation data characterizing the K- and Ka-band mobile satcom channel. In addition, observations from many of the application-specific experiments will also be provided.

  20. Hypervelocity microparticle characterization

    SciTech Connect

    Idzorek, G.C.

    1996-11-01

    To protect spacecraft from orbital debris requires a basic understanding of the processes involved in hypervelocity impacts and characterization of detectors to measure the space environment. Both require a source of well characterized hypervelocity particles. Electrostatic acceleration of charged microspheres provides such a source. Techniques refined at the Los Alamos National Laboratory provided information on hypervelocity impacts of particles of known mass and velocity ranging from 20-1000 nm diameter and 1-100 km/s. A Van De Graaff generator operating at 6 million volts was used to accelerate individual carbonyl iron microspheres produced by a specially designed particle source. Standard electrostatic lenses and steering were used to control the particles flight path. Charge sensitive pickoff tubes measured the particle charge and velocity in- flight without disturbing the particle. This information coupled with the measured Van De Graaff terminal voltage allowed calculation of the particle energy, mass, momenta and (using an assumed density) the size. Particles with the desired parameters were then electrostatically directed to a target chamber. Targets used in our experiments included cratering and foil puncture targets, microphone momentum enhancement detectors, triboluminescent detectors, and ``splash`` charge detectors. In addition the system has been used to rapidly characterize size distributions of conductive plastic particles and potentially provide a method of easily sorting microscopic particles by size.

  1. Thermal Energy Storage Flight Experiment in Microgravity

    NASA Technical Reports Server (NTRS)

    Namkoong, David

    1992-01-01

    The Thermal Energy Storage Flight Experiment was designed to characterize void shape and location in LiF-based phase change materials in different energy storage configurations representative of advanced solar dynamic systems. Experiment goals and payload design are described in outline and graphic form.

  2. Out-of-Body Experience and Autoscopy of Neurological Origin

    ERIC Educational Resources Information Center

    Blanke, Olaf; Landis, Theodor; Spinelli, Laurent; Seeck,Margitta

    2004-01-01

    During an out-of-body experience (OBE), the experient seems to be awake and to see his body and the world from a location outside the physical body. A closely related experience is autoscopy (AS), which is characterized by the experience of seeing one's body in extrapersonal space. Yet, despite great public interest and many case studies,…

  3. Initial conditions of radiative shock experiments

    SciTech Connect

    Kuranz, C. C.; Drake, R. P.; Krauland, C. M.; Marion, D. C.; Grosskopf, M. J.; Rutter, E.; Torralva, B.; Holloway, J. P.; Bingham, D.; Goh, J.; Boehly, T. R.; Sorce, A. T.

    2013-05-15

    We performed experiments at the Omega Laser Facility to characterize the initial, laser-driven state of a radiative shock experiment. These experiments aimed to measure the shock breakout time from a thin, laser-irradiated Be disk. The data are then used to inform a range of valid model parameters, such as electron flux limiter and polytropic γ, used when simulating radiative shock experiments using radiation hydrodynamics codes. The characterization experiment and the radiative shock experiment use a laser irradiance of ∼7 × 10{sup 14} W cm{sup −2} to launch a shock in the Be disk. A velocity interferometer and a streaked optical pyrometer were used to infer the amount of time for the shock to move through the Be disk. The experimental results were compared with simulation results from the Hyades code, which can be used to model the initial conditions of a radiative shock system using the CRASH code.

  4. Shooting Star Experiment

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The Shooting Star Experiment (SSE) is designed to develop and demonstrate the technology required to focus the Sun's energy and use the energy for inexpensive space propulsion research. Pictured is an engineering model (Pathfinder III) of SSE and its thermal vacuum test to simulate in-orbit conditions at the X-Ray Calibration Facility at the Marshall Space Flight Center (MSFC). This model was used to test and characterize the motion and deformation of the structure caused by thermal effects. In this photograph, alignment targets are being placed on the engineering model so that a theodolite (alignment telescope) could be used to accurately measure the deformation and deflection of the engineering model under extreme condition, such as the coldness of deep space and the hotness of the Sun, as well as vacuum.

  5. Serum activity of angiotensin converting enzyme 2 is decreased in patients with acute ischemic stroke.

    PubMed

    Bennion, Douglas M; Rosado, Christian A; Haltigan, Emily A; Regenhardt, Robert W; Sumners, Colin; Waters, Michael F

    2016-07-01

    Levels of angiotensin converting enzyme 2 (ACE2), a cardio and neuro-protective carboxypeptidase, are dynamically altered after stroke in preclinical models. We sought to characterize the previously unexplored changes in serum ACE2 activity of stroke patients and the mechanism of these changes. Serum samples were obtained from patients during acute ischemic stroke (n=39), conditions mimicking stroke (stroke-alert, n=23), or from control participants (n=20). Enzyme activity levels were analyzed by fluorometric assay and correlated with clinical variables by regression analyses. Serum ACE2 activity was significantly lower in acute ischemic stroke as compared to both control and stroke-alert patients, followed by an increase to control levels at three days. Serum ACE2 activity significantly correlated with the presence of ischemic stroke after controlling for other factors (P=0.01). Additional associations with ACE2 activity included a positive correlation with systolic blood pressure at presentation in stroke-alert (R(2)=0.24, P=0.03), while stroke levels showed no correlation (R(2)=0.01, P=0.50). ACE2 sheddase activity was unchanged between groups. These dynamic changes in serum ACE2 activity in stroke, which concur with preclinical studies, are not likely to be driven primarily by acute changes in blood pressure or sheddase activity. These findings provide new insight for developing therapies targeting this protective system in ischemic stroke. PMID:27488276

  6. Characterization of the energy resolution and the tracking capabilities of a hybrid pixel detector with CdTe-sensor layer for a possible use in a neutrinoless double beta decay experiment

    NASA Astrophysics Data System (ADS)

    Filipenko, Mykhaylo; Gleixner, Thomas; Anton, Gisela; Durst, Jürgen; Michel, Thilo

    2013-04-01

    Many different experiments are being developed to explore the existence of the neutrinoless double beta decay (0 νββ) since it would imply fundamental consequences for particle physics. In this work we present results on the evaluation of Timepix detectors with cadmium-telluride sensor material to search for 0 νββ in 116Cd. This work was carried out with the COBRA collaboration and the Medipix collaboration. Due to the relatively small pixel dimension of 110×110×1000 μm3 the energy deposited by particles typically extends over several detector pixels leading to a track in the pixel matrix. We investigated the separation power regarding different event-types like α-particles, atmospheric muons, single electrons and electron-positron pairs produced at a single vertex. We achieved excellent classification power for α-particles and muons. In addition, we achieved good separation power between single electron and electron-positron pair production events. These separation abilities indicate a very good background reduction for the 0 νββ search. Further, in order to distinguish between 2 νββ and 0 νββ, the energy resolution is of particular importance. We carried out simulations which demonstrate that an energy resolution of 0.43 % is achievable at the Q-value for 0 νββ of 116Cd at 2.814 MeV. We measured an energy resolution of 1.6 % at a nominal energy of 1589 keV for electron-positron tracks which is about two times worse that predicted by our simulations. This deviation is probably due to the problem of detector calibration at energies above 122 keV which is discussed in this paper as well.

  7. Spectral characterization of lithographic sources

    SciTech Connect

    Cerjan, C.

    1993-06-01

    Spectral data collected in recent laser-plasma experiments at LLNL for Sn are compared to simulation results in order to more fully characterize the plasma properties, especially electron temperature and density. These plasma conditions determine the ionization states achieved by the material and the consequent radiative emission. Synthetic spectra are produced using very detailed radiating line positions and oscillator strengths calculated from extensive multi-configuration Dirac-Fock computations. Better quantitative agreement with experimental conversion efficiencies in the laser intensity regime of interest to projection soft x-ray lithography is obtained using this atomic database. The spectral characterization thus validates the general reliability of the simulations.

  8. Pretest characterization of WIPP experimental waste

    SciTech Connect

    Johnson, J.; Davis, H.; Drez, P.E.; Devarakonda, M.

    1991-12-31

    The Waste Isolation Pilot Plant (WIPP) near Carlsbad, New Mexico, is an underground repository designed for the storage and disposal of transuranic (TRU) wastes from US Department of Energy (DOE) facilities across the country. The Performance Assessment (PA) studies for WIPP address compliance of the repository with applicable regulations, and include full-scale experiments to be performed at the WIPP site. These experiments are the bin-scale and alcove tests to be conducted by Sandia National Laboratories (SNL). Prior to conducting these experiments, the waste to be used in these tests needs to be characterized to provide data on the initial conditions for these experiments. This characterization is referred to as the Pretest Characterization of WIPP Experimental Waste, and is also expected to provide input to other programmatic efforts related to waste characterization. The purpose of this paper is to describe the pretest waste characterization activities currently in progress for the WIPP bin-scale waste, and to discuss the program plan and specific analytical protocols being developed for this characterization. The relationship between different programs and documents related to waste characterization efforts is also highlighted in this paper.

  9. CHARACTERIZATION OF DAMAGED MATERIALS

    SciTech Connect

    Hsu, P C; Dehaven, M; McClelland, M; Chidester, S; Maienschein, J L

    2006-06-23

    Thermal damage experiments were conducted on LX-04, LX-10, and LX-17 at high temperatures. Both pristine and damaged samples were characterized for their material properties. A pycnometer was used to determine sample true density and porosity. Gas permeability was measured in a newly procured system (diffusion permeameter). Burn rate was measured in the LLNL strand burner. Weight losses upon thermal exposure were insignificant. Damaged pressed parts expanded, resulting in a reduction of bulk density by up to 10%. Both gas permeabilities and burn rates of the damaged samples increased by several orders of magnitude due to higher porosity and lower density. Moduli of the damaged materials decreased significantly, an indication that the materials became weaker mechanically. Damaged materials were more sensitive to shock initiation at high temperatures. No significant sensitization was observed when the damaged samples were tested at room temperature.

  10. Glass sample characterization

    NASA Technical Reports Server (NTRS)

    Ahmad, Anees

    1990-01-01

    The development of in-house integrated optical performance modelling capability at MSFC is described. This performance model will take into account the effects of structural and thermal distortions, as well as metrology errors in optical surfaces to predict the performance of large an complex optical systems, such as Advanced X-Ray Astrophysics Facility. The necessary hardware and software were identified to implement an integrated optical performance model. A number of design, development, and testing tasks were supported to identify the debonded mirror pad, and rebuilding of the Technology Mirror Assembly. Over 300 samples of Zerodur were prepared in different sizes and shapes for acid etching, coating, and polishing experiments to characterize the subsurface damage and stresses produced by the grinding and polishing operations.

  11. Situating emotional experience

    PubMed Central

    Wilson-Mendenhall, Christine D.; Barrett, Lisa Feldman; Barsalou, Lawrence W.

    2013-01-01

    Psychological construction approaches to emotion suggest that emotional experience is situated and dynamic. Fear, for example, is typically studied in a physical danger context (e.g., threatening snake), but in the real world, it often occurs in social contexts, especially those involving social evaluation (e.g., public speaking). Understanding situated emotional experience is critical because adaptive responding is guided by situational context (e.g., inferring the intention of another in a social evaluation situation vs. monitoring the environment in a physical danger situation). In an fMRI study, we assessed situated emotional experience using a newly developed paradigm in which participants vividly imagine different scenarios from a first-person perspective, in this case scenarios involving either social evaluation or physical danger. We hypothesized that distributed neural patterns would underlie immersion in social evaluation and physical danger situations, with shared activity patterns across both situations in multiple sensory modalities and in circuitry involved in integrating salient sensory information, and with unique activity patterns for each situation type in coordinated large-scale networks that reflect situated responding. More specifically, we predicted that networks underlying the social inference and mentalizing involved in responding to a social threat (in regions that make up the “default mode” network) would be reliably more active during social evaluation situations. In contrast, networks underlying the visuospatial attention and action planning involved in responding to a physical threat would be reliably more active during physical danger situations. The results supported these hypotheses. In line with emerging psychological construction approaches, the findings suggest that coordinated brain networks offer a systematic way to interpret the distributed patterns that underlie the diverse situational contexts characterizing emotional

  12. Situating emotional experience.

    PubMed

    Wilson-Mendenhall, Christine D; Barrett, Lisa Feldman; Barsalou, Lawrence W

    2013-01-01

    Psychological construction approaches to emotion suggest that emotional experience is situated and dynamic. Fear, for example, is typically studied in a physical danger context (e.g., threatening snake), but in the real world, it often occurs in social contexts, especially those involving social evaluation (e.g., public speaking). Understanding situated emotional experience is critical because adaptive responding is guided by situational context (e.g., inferring the intention of another in a social evaluation situation vs. monitoring the environment in a physical danger situation). In an fMRI study, we assessed situated emotional experience using a newly developed paradigm in which participants vividly imagine different scenarios from a first-person perspective, in this case scenarios involving either social evaluation or physical danger. We hypothesized that distributed neural patterns would underlie immersion in social evaluation and physical danger situations, with shared activity patterns across both situations in multiple sensory modalities and in circuitry involved in integrating salient sensory information, and with unique activity patterns for each situation type in coordinated large-scale networks that reflect situated responding. More specifically, we predicted that networks underlying the social inference and mentalizing involved in responding to a social threat (in regions that make up the "default mode" network) would be reliably more active during social evaluation situations. In contrast, networks underlying the visuospatial attention and action planning involved in responding to a physical threat would be reliably more active during physical danger situations. The results supported these hypotheses. In line with emerging psychological construction approaches, the findings suggest that coordinated brain networks offer a systematic way to interpret the distributed patterns that underlie the diverse situational contexts characterizing emotional life

  13. Photoelectronic characterization of heterointerfaces.

    SciTech Connect

    Brumbach, Michael Todd

    2012-02-01

    In many devices such as solar cells, light emitting diodes, transistors, etc., the performance relies on the electronic structure at interfaces between materials within the device. The objective of this work was to perform robust characterization of hybrid (organic/inorganic) interfaces by tailoring the interfacial region for photoelectron spectroscopy. Self-assembled monolayers (SAM) were utilized to induce dipoles of various magnitudes at the interface. Additionally, SAMs of molecules with varying dipolar characteristics were mixed into spatially organized structures to systematically vary the apparent work function. Polymer thin films were characterized by depositing films of varying thicknesses on numerous substrates with and without interfacial modifications. Hard X-ray photoelectron spectroscopy (HAXPES) was performed to evaluate a buried interface between indium tin oxide (ITO), treated under various conditions, and poly(3-hexylthiophene) (P3HT). Conducting polymer films were found to be sufficiently conducting such that no significant charge redistribution in the polymer films was observed. Consequently, a further departure from uniform substrates was taken whereby electrically disconnected regions of the substrate presented ideally insulating interfacial contacts. In order to accomplish this novel strategy, interdigitated electrodes were used as the substrate. Conducting fingers of one half of the electrodes were electrically grounded while the other set of electrodes were electronically floating. This allowed for the evaluation of substrate charging on photoelectron spectra (SCOPES) in the presence of overlying semiconducting thin films. Such an experiment has never before been reported. This concept was developed out of the previous experiments on interfacial modification and thin film depositions and presents new opportunities for understanding chemical and electronic changes in a multitude of materials and interfaces.

  14. Apparatus and method for characterizing ultrafast polarization varying optical pulses

    DOEpatents

    Smirl, A.; Trebino, R.P.

    1999-08-10

    Practical techniques are described for characterizing ultrafast potentially ultraweak, ultrashort optical pulses. The techniques are particularly suited to the measurement of signals from nonlinear optical materials characterization experiments, whose signals are generally too weak for full characterization using conventional techniques. 2 figs.

  15. Southern Ocean Iron Experiment (SOFex)

    SciTech Connect

    Coale, Kenneth H.

    2005-07-28

    The Southern Ocean Iron Experiment (SOFeX) was an experiment decades in the planning. It's implementation was among the most complex ship operations that SIO has been involved in. The SOFeX field expedition was successful in creating and tracking two experimentally enriched areas of the Southern Ocean, one characterized by low silicic acid, one characterized by high silicic acid. Both experimental sites were replete with abundant nitrate. About 100 scientists were involved overall. The major findings of this study were significant in several ways: (1) The productivity of the southern ocean is limited by iron availability. (2) Carbon uptake and flux is therefore controlled by iron availability (3) In spite of low silicic acid, iron promotes non-silicious phytoplankton growth and the uptake of carbon dioxide. (4) The transport of fixed carbon from the surface layers proceeds with a C:N ratio that would indicate differential remineralization of nitrogen at shallow depths. (5) These finding have major implications for modeling of carbon export based on nitrate utilization. (6) The general results of the experiment indicate that, beyond other southern ocean enrichment experiments, iron inputs have a much wider impact of productivity and carbon cycling than previously demonstrated. Scientific presentations: Coale, K., Johnson, K, Buesseler, K., 2002. The SOFeX Group. Eos. Trans. AGU 83(47) OS11A-0199. Coale, K., Johnson, K. Buesseler, K., 2002. SOFeX: Southern Ocean Iron Experiments. Overview and Experimental Design. Eos. Trans. AGU 83 (47) OS22D-01. Buesseler, K.,et al. 2002. Does Iron Fertilization Enhance Carbon Sequestration? Particle flux results from the Southern Ocean Iron Experiment. Eos. Trans. AGU 83 (47), OS22D-09. Johnson, K. et al. 2002. Open Ocean Iron Fertilization Experiments From IronEx-I through SOFeX: What We Know and What We Still Need to Understand. Eos. Trans. AGU 83 (47), OS22D-12. Coale, K. H., 2003. Carbon and Nutrient Cycling During the Southern

  16. Electrochemical Characterization Laboratory (Fact Sheet)

    SciTech Connect

    Not Available

    2011-10-01

    This fact sheet describes the purpose, lab specifications, applications scenarios, and information on how to partner with NREL's Electrochemical Characterization Laboratory at the Energy Systems Integration Facility. The research focus at the Electrochemical Characterization Laboratory at NREL's Energy Systems Integration Facility (ESIF) is evaluating the electrochemical properties of novel materials synthesized by various techniques and understanding and delineating the reaction mechanisms to provide practical solutions to PEMFCs commercialization issues of cost, performance and durability. It is also involved in the development of new tools and techniques for electrochemical characterization. The laboratory concentrates on the development and characterization of new materials for PEMFCs such as electrocatalysts, catalyst supports in terms of electrochemical activity, electrochemical surface area and corrosion/durability. The impact of impurities and/or contaminants on the catalyst activity is also under study. Experiments that can be performed include: (1) Determination and benchmarking of novel electrocatalyst activity; (2) Determination of electrochemical surface area; (3) Determination of electrocatalyst and support corrosion resistance and durability; (4) Synthesis and characterization of novel electrocatalyst; (5) Determination of fundamental electrochemical parameters; and (6) Estimation of electrocatalyst utilization.

  17. Sleep Monitoring Experiment - Skylab Experiment M133

    NASA Technical Reports Server (NTRS)

    1970-01-01

    This 1970 photograph shows equipment for the Skylab's Sleep Monitoring Experiment (M133), a medical evaluation designed to objectively determine the amount and quality of crewmembers' inflight sleep. The experiment monitored and recorded electroencephalographic (EEG) and electrooculographic (EOG) activity during astronauts' sleep periods. One of the astronauts was selected for this experiment and wore a fitted cap during his sleep periods. The Marshall Space Flight Center had program management responsibility for the development of Skylab hardware and experiments.

  18. Generation and characterization of aerosols and vapors for inhalation experiments.

    PubMed Central

    Tillery, M I; Wood, G O; Ettinger, H J

    1976-01-01

    Control of aerosol and vapor characteristics that affect the toxicity of inhaled contaminants often determines the methods of generating exposure atmospheres. Generation methods for aerosols and vapors are presented. The characteristics of the resulting exposure atmosphere and the limitations of the various generation methods are discussed. Methods and instruments for measuring the airborne contaminant with respect to various charcteristics are also described. PMID:797565

  19. Characterizing donation behavior from psychophysiological indices of narrative experience

    PubMed Central

    Correa, Kelly A.; Stone, Bradly T.; Stikic, Maja; Johnson, Robin R.; Berka, Chris

    2015-01-01

    Research on narrative persuasion has yet to investigate whether this process influences behavior. The current study explored whether: (1) a narrative could persuade participants to donate to a charity, a prosocial, behavioral decision; (2) psychophysiological metrics can delineate the differences between donation/non-donation behaviors; and (3) donation behavior can be correlated with measures of psychophysiology, self-reported reactions to the narrative, and intrinsic characteristics. Participants (n = 49) completed personality/disposition questionnaires, viewed one of two versions of a narrative while EEG and ECG were recorded, completed a questionnaire regarding their reactions to the narrative, and were given an opportunity to donate to a charity related to the themes of the narrative. Results showed that: (1) 34.7% of participants donated; (2) psychophysiological metrics successfully delineated between donation behaviors and the effects of narrative version; and (3) psychophysiology and reactions to the narrative were better able to explain the variance (88 and 65%, respectively) in the amount donated than all 3 metrics combined as well as any metric alone. These findings demonstrate the promise of narrative persuasion for influencing prosocial, behavioral decisions. Our results also illustrate the utility of the previously stated metrics for understanding and possibly even manipulating behaviors resulting from narrative persuasion. PMID:26379488

  20. AEROSOL CHARACTERIZATION WITH CENTRIFUCAL AEROSOL SPECTROMETERS: THEORY AND EXPERIMENT

    EPA Science Inventory

    A general mathematical model describing the motion of particles in aerosol centrifuges has been developed. t has been validated by comparisons of theoretically predicted calibration sites with experimental data from tests sizing aerosols in instruments of three different spiral d...

  1. Mars aqueous chemistry experiment

    NASA Technical Reports Server (NTRS)

    Clark, Benton C.; Mason, Larry W.

    1993-01-01

    The Mars Aqueous Chemistry Experiment (MACE) is designed to conduct a variety of measurements on regolith samples, encompassing mineral phase analyses, chemical interactions with H2O, and physical properties determinations. From these data, much can be learned or inferred regarding the past weathering environment, the contemporaneous soil micro-environments, and the general chemical and physical state of the Martian regolith. By analyzing both soil and duricrust samples, the nature of the latter may become more apparent. Sites may be characterized for comparative purposes and criteria could be set for selection of high priority materials on future sample return missions. Progress for the first year MACE PIDDP is reported in two major areas of effort: (1) fluids handling concepts, definition, and breadboard fabrication and (2) aqueous chemistry ion sensing technology and test facility integration. A fluids handling breadboard was designed, fabricated, and tested at Mars ambient pressure. The breadboard allows fluid manipulation scenarios to be tested under the reduced pressure conditions expected in the Martian atmosphere in order to validate valve operations, orchestrate analysis sequences, investigate sealing integrity, and to demonstrate efficacy of the fluid handling concept. Additional fluid manipulation concepts have also been developed based on updated MESUR spacecraft definition. The Mars Aqueous Chemistry Experiment Ion Selective Electrode (ISE) facility was designed as a test bed to develop a multifunction interface for measurements of chemical ion concentrations in aqueous solution. The interface allows acquisition of real time data concerning the kinetics and heats of salt dissolution, and transient response to calibration and solubility events. An array of ion selective electrodes has been interfaced and preliminary calibration studies performed.

  2. Monitoring hydraulic fracture growth: Laboratory experiments

    SciTech Connect

    Groenenboom, J.; Dam, D.B. van

    2000-04-01

    The authors carry out small-scale hydraulic fracture experiments to investigate the physics of hydraulic fracturing. The laboratory experiments are combined with time-lapse ultrasonic measurements with active sources using both compressional and shear-wave transducers. For the time-lapse measurements they focus on ultrasonic measurement changes during fracture growth. As a consequence they can detect the hydraulic fracture and characterize its shape and geometry during growth. Hence, this paper deals with fracture characterization using time-lapse acoustic data. Hydraulic fracturing is used in the oil and gas industry to stimulate reservoir production.

  3. Novice Counselors' Conceptualizations and Experiences of Therapeutic Relationships

    ERIC Educational Resources Information Center

    Schwing, Alison E.; LaFollette, Julie R.; Steinfeldt, Jesse A.; Wong, Y. Joel

    2011-01-01

    This qualitative study investigated three novice counselors' experiences and characterizations of therapeutic relationships. Thematic analyses of interviews and diaries revealed six common themes: (a) the centrality of supervision and training experiences to navigating interpersonal experiences with clients; (b) anxiety about counselors' roles in…

  4. Prevalence and Prognostic Significance of Hyponatremia in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease: Data from the Akershus Cardiac Examination (ACE) 2 Study

    PubMed Central

    Brynildsen, Jon; Høiseth, Arne Didrik; Følling, Ivar; Brekke, Pål H.; Christensen, Geir; Hagve, Tor-Arne; Verbalis, Joseph G.; Omland, Torbjørn; Røsjø, Helge

    2016-01-01

    Background Hyponatremia is prevalent and associated with mortality in patients with heart failure (HF). The prevalence and prognostic implications of hyponatremia in acute exacerbation of chronic obstructive pulmonary (AECOPD) have not been established. Method We included 313 unselected patients with acute dyspnea who were categorized by etiology of dyspnea according to established guidelines (derivation cohort). Serum Na+ was determined on hospital admission and corrected for hyperglycemia, and hyponatremia was defined as [Na+]<137 mmol/L. Survival was ascertained after a median follow-up of 816 days and outcome was analyzed in acute HF (n = 143) and AECOPD (n = 83) separately. Results were confirmed in an independent AECOPD validation cohort (n = 99). Results In the derivation cohort, median serum Na+ was lower in AECOPD vs. acute HF (138.5 [135.9–140.5] vs. 139.2 [136.7–141.3] mmol/L, p = 0.02), while prevalence of hyponatremia (27% [22/83] vs. 20% [29/143], p = 0.28) and mortality rate (42% [35/83] vs. 46% [66/143], p = 0.56) were similar. By univariate Cox regression analysis, hyponatremia was associated with increased mortality in acute HF (HR 1.85 [95% CI 1.08, 3.16], p = 0.02), but not in AECOPD (HR 1.00 [0.47, 2.15], p = 1.00). Analogous to the results of the derivation cohort, hyponatremia was prevalent also in the AECOPD validation cohort (25% [25/99]), but not associated with mortality. The diverging effect of hyponatremia on outcome between AECOPD and acute HF was statistically significant (p = 0.04). Conclusion Hyponatremia is prevalent in patients with acute HF and AECOPD, but is associated with mortality in patients with acute HF only. PMID:27529844

  5. Optimizing New Dark Energy Experiments

    SciTech Connect

    Tyson, J. Anthony

    2013-08-26

    Next generation “Stage IV” dark energy experiments under design during this grant, and now under construction, will enable the determination of the properties of dark energy and dark matter to unprecedented precision using multiple complementary probes. The most pressing challenge in these experiments is the characterization and understanding of the systematic errors present within any given experimental configuration and the resulting impact on the accuracy of our constraints on dark energy physics. The DETF and the P5 panel in their reports recommended “Expanded support for ancillary measurements required for the long-term program and for projects that will improve our understanding and reduction of the dominant systematic measurement errors.” Looking forward to the next generation Stage IV experiments we have developed a program to address the most important potential systematic errors within these experiments. Using data from current facilities it has been feasible and timely to undertake a detailed investigation of the systematic errors. In this DOE grant we studied of the source and impact of the dominant systematic effects in dark energy measurements, and developed new analysis tools and techniques to minimize their impact. Progress under this grant is briefly reviewed in this technical report. This work was a necessary precursor to the coming generations of wide-deep probes of the nature of dark energy and dark matter. The research has already had an impact on improving the efficiencies of all Stage III and IV dark energy experiments.

  6. The characterization of fluorinated graphite

    SciTech Connect

    Hagaman, E.W.; Gakh, A.A.; Annis, B.K.

    1995-12-31

    The characterization of solid fossil fuels by chemical and spectroscopic methods requires extensive modelling in less complex systems for chemical proof of principle and technique development. In previous work coal was fluorinated with dilute, elemental fluorine under conditions that were expected to lead to materials that contain only fluoromethine moieties. The solid state, cross polarization/magic angle spinning (CP/MAS) {sup 13}C NMR spectra of the fluorinated coal are complex, indicating more chemical modification than originally anticipated. Our goal in the coal derivatization was to sequentially increase the severity of the fluorination and observe by {sup 19}F and {sup 13}C NMR the type and concentration of fluorine functional groups created in the coal milieu. This requires the ability to discriminate between C, CF, CF{sub 2}, and CF, moieties in the coal matrix. The task can be accomplished by implementing the spectral editing technique of Wu and Zilm which distinguishes different kinds of carbon resonances, especially CH and CH{sub 2} resonances. These experiments utilize cross polarization (CP) and polarization inversion (PI) to effect the discrimination. Our version of this experiment is a triple resonance experiment that incorporates {sup 19}F-{sup 13}C CP, PI, and simultaneous {sup 1}H and {sup 19}F dipolar decoupling. In order to evaluate the elemental fluorine chemistry in a matrix simpler than coal, fluorinated graphite was prepared. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface species, i.e., count CF, CF{sub 2} and CF{sub 3} species. These well-characterized samples are the models we will use to test the NIVIR editing experiments. The XPS and atomic force microscopy (AFM) data on the first fluorinated graphites we have prepared are reported in this paper.

  7. Mars aqueous chemistry experiment

    NASA Technical Reports Server (NTRS)

    Clark, Benton C.; Mason, Larry W.

    1994-01-01

    Mars Aqueous Chemistry Experiment (MACE) is designed to conduct a variety of measurements on regolith samples, encompassing mineral phase analyses, chemical interactions with H2O, and physical properties determinations. From these data, much can be learned or inferred regarding the past weathering environment, the contemporaneous soil micro-environments, and the general chemical and physical state of the Martian regolith. By analyzing both soil and duricrust samples, the nature of the latter may become more apparent. Sites may be characterized for comparative purposes and criteria could be set for selection of high priority materials on future sample return missions. The second year of the MACE project has shown significant progress in two major areas. MACE Instrument concept definition is a baseline design that has been generated for the complete MACE instrument, including definition of analysis modes, mass estimates and thermal model. The design includes multiple reagent reservoirs, 10 discrete analysis cells, sample manipulation capability, and thermal control. The MACE Measurement subsystems development progress is reported regarding measurement capabilities for aqueous ion sensing, evolved gas sensing, solution conductivity measurement, reagent addition (titration) capabilities, and optical sensing of suspended particles.

  8. The trapped human experiment.

    PubMed

    Huo, R; Agapiou, A; Bocos-Bintintan, V; Brown, L J; Burns, C; Creaser, C S; Devenport, N A; Gao-Lau, B; Guallar-Hoyas, C; Hildebrand, L; Malkar, A; Martin, H J; Moll, V H; Patel, P; Ratiu, A; Reynolds, J C; Sielemann, S; Slodzynski, R; Statheropoulos, M; Turner, M A; Vautz, W; Wright, V E; Thomas, C L P

    2011-12-01

    This experiment observed the evolution of metabolite plumes from a human trapped in a simulation of a collapsed building. Ten participants took it in turns over five days to lie in a simulation of a collapsed building and eight of them completed the 6 h protocol while their breath, sweat and skin metabolites were passed through a simulation of a collapsed glass-clad reinforced-concrete building. Safety, welfare and environmental parameters were monitored continuously, and active adsorbent sampling for thermal desorption GC-MS, on-line and embedded CO, CO(2) and O(2) monitoring, aspirating ion mobility spectrometry with integrated semiconductor gas sensors, direct injection GC-ion mobility spectrometry, active sampling thermal desorption GC-differential mobility spectrometry and a prototype remote early detection system for survivor location were used to monitor the evolution of the metabolite plumes that were generated. Oxygen levels within the void simulator were allowed to fall no lower than 19.1% (v). Concurrent levels of carbon dioxide built up to an average level of 1.6% (v) in the breathing zone of the participants. Temperature, humidity, carbon dioxide levels and the physiological measurements were consistent with a reproducible methodology that enabled the metabolite plumes to be sampled and characterized from the different parts of the experiment. Welfare and safety data were satisfactory with pulse rates, blood pressures and oxygenation, all within levels consistent with healthy adults. Up to 12 in-test welfare assessments per participant and a six-week follow-up Stanford Acute Stress Response Questionnaire indicated that the researchers and participants did not experience any adverse effects from their involvement in the study. Preliminary observations confirmed that CO(2), NH(3) and acetone were effective markers for trapped humans, although interactions with water absorbed in building debris needed further study. An unexpected observation from the NH(3

  9. The ArgoNeuT experiment

    SciTech Connect

    Spitz, J.; /Yale U.

    2010-01-01

    ArgoNeuT is a Liquid Argon Time Projection Chamber neutrino experiment that recently completed its physics run in the NuMI beamline at Fermilab. Along with research and design for future LArTPCs, the experiments goals include performing a number of neutrino and anti-neutrino cross section measurements. Also, ArgoNeuT hopes to further the understanding of the nuclear physics involved in neutrino scattering by characterizing the low energy protons created in such interactions.

  10. Wireless experiments on a Motorola mesh testbed.

    SciTech Connect

    Riblett, Loren E., Jr.; Wiseman, James M.; Witzke, Edward L.

    2010-06-01

    Motomesh is a Motorola product that performs mesh networking at both the client and access point levels and allows broadband mobile data connections with or between clients moving at vehicular speeds. Sandia National aboratories has extensive experience with this product and its predecessors in infrastructure-less mobile environments. This report documents experiments, which characterize certain aspects of how the Motomesh network performs when obile units are added to a fixed network infrastructure.

  11. Description of the Joint Damping Experiment (JDX)

    NASA Technical Reports Server (NTRS)

    Folkman, Steven L.; Redd, Frank J.

    1992-01-01

    The overall objective is to develop a small-scale shuttle flight experiment which allows researchers to: (1) characterize the influence of gravity and joint gaps on a small-scale truss model, and (2) evaluate the applicability of low-g aircraft test results for predicting on-orbit behavior. The experiment consists of a three-bay truss and associated hardware for truss excitation and measurement of oscillations. Other aspects of the investigation are presented in viewgraph form.

  12. Notes on Experiments.

    ERIC Educational Resources Information Center

    Physics Education, 1989

    1989-01-01

    Described are three physics experiments: (1) "Holographic Prism"; (2) "Teaching about Energy with the Gravicar"; and (3) "The Coherer." In each experiment, a brief description of the experiment devised is provided with diagrams and references. (YP)

  13. Notes on Experiments.

    ERIC Educational Resources Information Center

    Physics Education, 1984

    1984-01-01

    Describes: (1) experiments using a simple phonocardiograph; (2) radioactivity experiments involving a VELA used as a ratemeter; (3) a 25cm continuously operating Foucault pendulum; and (4) camera control of experiments. Descriptions of equipment needed are provided when applicable. (JN)

  14. Materials Characterization Laboratory (Fact Sheet)

    SciTech Connect

    Not Available

    2011-10-01

    This fact sheet describes the purpose, lab specifications, applications scenarios, and information on how to partner with NREL's Materials Characterization Laboratory at the Energy Systems Integration Facility. The Materials Characterization Laboratory at NREL's Energy Systems Integration Facility (ESIF) research focus is the physical and photoelectrochemical characterization of novel materials. In this laboratory unknown samples are characterized by identifying and quantifying molecular species present through the implementation of a suite of analytical instrumentation and techniques. This leads to the ability to deconvolute decomposition routes and elucidate reaction mechanisms of materials through thermal and evolved gas analysis. This aids in the synthesis of next generation materials that are tailored to optimize stability and performance. These techniques and next generation materials will have many applications. One particular focus is the stable and conductive tetherable cations for use as membrane materials in anion exchange membrane fuel cells. Another is to understand the leachant contaminants derived from balance of plant materials used in proton exchange membrane fuel cell vehicles. Once identified and quantified, these organic and ionic species are dosed as contaminants into ex/in-situ fuel cell tests, to determine the effect on durability and performance. This laboratory also acts in support of fuel cell catalysis, manufacturing, and other related projects. The Materials Characterization Laboratory will cover multiple analytical operations, with the overall goal of troubleshooting synthetic materials or process streams to improve performance. Having novel evolved gas analysis and other analytical capabilities; this laboratory provides a viable location to analyze small batch samples, whereas setting up these types of capabilities and expertise would be cost and time prohibitive for most institutions. Experiments that can be performed include: (1

  15. Measurements and characterization - Electro-optical characterization

    SciTech Connect

    Cook, G.

    2000-03-16

    This brochure presents the capabilities that the Measurements and Characterization Division has in Electro-Optical Characterization, in which a variety of spectroscopy, ellipsometry, and capacitance techniques are used to probe the fundamental electrical and optical properties of solid-state materials.

  16. Cobalt Alums. A Demonstration Experiment

    NASA Astrophysics Data System (ADS)

    Schäffer, Claus E.; Steenberg, Paul

    2002-08-01

    The demonstration experiment describes the isolation of [Co(H2O)6]3+ both as the pure blue crystalline cesium cobalt alum, CsCo(SO4)2·12H2O, and as a light greenish-blue solid solution of ammonium cobalt alum in (NH4)Al(SO4)2·12H2O. The hexaaquacobalt(III) ion is prepared chemically by oxidation with hydrogen peroxide, taking advantage of the stabilization of CoIII relative to CoII by complexation with the carbonate ligand. A brief description of alum structure and a characterization of alum subclasses are included.

  17. Results from the HARP Experiment

    SciTech Connect

    Catanesi, M. G.

    2008-02-21

    Hadron production is a key ingredient in many aspects of {nu} physics. Precise prediction of atmospheric {nu} fluxes, characterization of accelerator {nu} beams, quantification of {pi} production and capture for {nu}-factory designs, all of these would profit from hadron production measurements. HARP at the CERN PS was the first hadron production experiment designed on purpose to match all these requirements. It combines a large, full phase space acceptance with low systematic errors and high statistics. HARP was operated in the range from 3 GeV to 15 GeV. We briefly describe here the most recent results.

  18. RF breakdown experiments at SLAC

    SciTech Connect

    Laurent, L.; Vlieks, A.; Pearson, C.; Caryotakis, G.; Luhmann, N.C.

    1999-05-01

    RF breakdown is a critical issue in the conditioning of klystrons, accelerator sections, and rf components for the next linear collider (NLC), as well as other high gradient accelerators and high power microwave sources. SLAC is conducting a series of experiments using an X-band traveling wave ring to characterize the processes and trigger mechanisms associated with rf breakdown. The goal of the research is to identify materials, processes, and manufacturing methods that will increase the breakdown threshold and minimize the time required for conditioning. {copyright} {ital 1999 American Institute of Physics.}

  19. Results from the HARP Experiment

    NASA Astrophysics Data System (ADS)

    Catanesi, M. G.

    2008-02-01

    Hadron production is a key ingredient in many aspects of ν physics. Precise prediction of atmospheric ν fluxes, characterization of accelerator ν beams, quantification of π production and capture for ν-factory designs, all of these would profit from hadron production measurements. HARP at the CERN PS was the first hadron production experiment designed on purpose to match all these requirements. It combines a large, full phase space acceptance with low systematic errors and high statistics. HARP was operated in the range from 3 GeV to 15 GeV. We briefly describe here the most recent results.

  20. Results from the HARP experiment

    NASA Astrophysics Data System (ADS)

    Catanesi, M. G.

    2007-06-01

    Hadron production is a key ingredient in many aspects of ν physics. Precise prediction of atmospheric ν fluxes, characterization of accelerator ν beams, quantification of π production and capture for ν-factory designs, all of these would profit from hadron production measurements. HARP at the CERN PS was the first hadron production experiment designed on purpose to match all these requirements. It combines a large, full phase space acceptance with low systematic errors and high statistics. HARP was operated in the range from 3 GeV to 15 GeV. We briefly describe here the most recent results.

  1. Railgun plasma armature characterisation experiments

    NASA Astrophysics Data System (ADS)

    Richardson, D. D.

    1984-05-01

    A plasma armature characterization experiment (PACE) is described. The PACE device is designed to study the plasma armature of railguns and yields information on properties such as temperature, pressure, densities, plasma potential, and ion species with their degrees of ionization. The main experimental studies are by spectroscopy of the light emitted and by Langmuir probes. The device simulates the plasma moving behind the projectile in a railgun by a static plasma held by electromagnetic forces against a fixed wall. Results to date demonstrate the feasibility of the concept and indicate improvements to the device which ensure that much useful information on railgun plasmas is forthcoming.

  2. The DAMIC Dark Matter Experiment

    SciTech Connect

    de Mello Neto, J. R.T.

    2015-10-07

    The DAMIC (DArk Matter In CCDs) experiment uses high-resistivity, scientific-grade CCDs to search for dark matter. The CCD’s low electronic noise allows an unprecedently low energy threshold of a few tens of eV; this characteristic makes it possible to detect silicon recoils resulting from interactions of low-mass WIMPs. In addition, the CCD’s high spatial resolution and the excellent energy response results in very effective background identification techniques. The experiment has a unique sensitivity to dark matter particles with masses below 10 GeV/c2. Previous results have motivated the construction of DAMIC100, a 100 grams silicon target detector currently being installed at SNOLAB. The mode of operation and unique imaging capabilities of the CCDs, and how they may be exploited to characterize and suppress backgrounds are discussed, as well as physics results after one year of data taking.

  3. Characterization of surfaces

    NASA Technical Reports Server (NTRS)

    Vanalstine, James M.

    1992-01-01

    Low gravity biotechnology experiments indicate a need to better understand and control a host of liquid-solid interfacial phenomena which reduce the efficiency of bioseparations methods on earth as well as in space. We have improved and utilized polymeric and silane derivatives, developed in association with MSFC, in order to control such phenomena. The objectives of the proposed research have been obtained. They were to improve NASA-patented coatings capable of controlling macromolecular adsorption, electroosmosis, and particle electrophoresis over a wide range of pH, and to further characterize the ability of polymeric coatings to control wall wetting interactions. To date this research has resulted in six publications and four abstracts. It has also aided researchers at MSFC with studies on the electrophoresis of large DNA molecules in free solution. It will continue to enhance NASA's efforts to exploit the space environment to enhance knowledge of phenomena relevant to biotechnology, and obtain bioseparations currently unobtainable on Earth. Abstracts from the 1994 ACS Meeting in Birmingham are attached.

  4. A Kinetic Experiment for the Biochemistry Laboratory.

    ERIC Educational Resources Information Center

    Palmer, Richard E.

    1986-01-01

    Discusses the use of specific reactions of metabolic pathways to make measurements in the laboratory. Describes an adaptation of an experiment used in undergraduate biochemistry laboratories involving the induction of an enzyme in E. coli, as well as its partial purification and characterization. (TW)

  5. Lessons From Two Decades of FACE Experiments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plant responses to the projected future levels of CO2 were first characterized in short term experiments lasting days to weeks. However, longer term acclimation responses to elevated CO2 were subsequently discovered to be very important in determining plant and ecosystem function. Free-Air CO2 Enric...

  6. Liner target interaction experiments on Pegasus II

    SciTech Connect

    Hockaday, M.P.; Chrien, R.E.; Bartsch, R.

    1995-09-01

    The Los Alamos High Energy Density Physics program uses capacitively driven low voltage, inductive-storage pulse power to implode cylindrical targets for hydrodynamics experiments. Once a precision driver liner was characterized an experimental series characterizing the aluminum target dynamics was performed. The target was developed for shock-induced quasi-particle ejecta experiments including holography. The concept for the Liner shock experiment is that the driver liner is used to impact the target liner which then accelerates toward a collimator with a slit in it. A shock wave is set up in the target liner and as the shock emerges from the back side of the target liner, ejecta are generated. By taking a laser hologram the particle distribution of the ejecta are hoped to be determined. The goal for the second experimental series was to characterize the target dynamics and not to measure and generate the ejecta. Only the results from the third shot, Pegasus II-26 fired April 26th, 1994, from the series is discussed in detail. The second experimental series successfully characterized the target dynamics necessary to move forward towards the planned quasi-ejecta experiments.

  7. The User Experience

    ERIC Educational Resources Information Center

    Schmidt, Aaron

    2010-01-01

    User experience (UX) is about arranging the elements of a product or service to optimize how people will interact with it. In this article, the author talks about the importance of user experience and discusses the design of user experiences in libraries. He first looks at what UX is. Then he describes three kinds of user experience design: (1)…

  8. Structural Assembly Demonstration Experiment (SADE) experiment design

    NASA Technical Reports Server (NTRS)

    Akin, D. L.; Bowden, M. L.

    1982-01-01

    The Structural Assembly Demonstration Experiment concept is to erect a hybrid deployed/assembled structure as an early space experiment in large space structures technology. The basic objectives can be broken down into three generic areas: (1) by performing assembly tasks both in space and in neutral buoyancy simulation, a mathematical basis will be found for the validity conditions of neutral buoyancy, thus enhancing the utility of water as a medium for simulation of weightlessness; (2) a data base will be established describing the capabilities and limitations of EVA crewmembers, including effects of such things as hardware size and crew restraints; and (3) experience of the M.I.T. Space Systems Lab in neutral buoyancy simulation of large space structures assembly indicates that the assembly procedure may create the largest loads that a structure will experience during its lifetime. Data obtained from the experiment will help establish an accurate loading model to aid designers of future space structures.

  9. Commercial Biomedical Experiments Payload

    NASA Technical Reports Server (NTRS)

    2003-01-01

    Experiments to seek solutions for a range of biomedical issues are at the heart of several investigations that will be hosted by the Commercial Instrumentation Technology Associates (ITA), Inc. The biomedical experiments CIBX-2 payload is unique, encompassing more than 20 separate experiments including cancer research, commercial experiments, and student hands-on experiments from 10 schools as part of ITA's ongoing University Among the stars program. Here, Astronaut Story Musgrave activates the CMIX-5 (Commercial MDA ITA experiment) payload in the Space Shuttle mid deck during the STS-80 mission in 1996 which is similar to CIBX-2. The experiments are sponsored by NASA's Space Product Development Program (SPD).

  10. Commercial Biomedical Experiments

    NASA Technical Reports Server (NTRS)

    2003-01-01

    Experiments to seek solutions for a range of biomedical issues are at the heart of several investigations that will be hosted by the Commercial Instrumentation Technology Associates (ITA), Inc. Biomedical Experiments (CIBX-2) payload. CIBX-2 is unique, encompassing more than 20 separate experiments including cancer research, commercial experiments, and student hands-on experiments from 10 schools as part of ITA's ongoing University Among the Stars program. Valerie Cassanto of ITA checks the Canadian Protein Crystallization Experiment (CAPE) carried by STS-86 to Mir in 1997. The experiments are sponsored by NASA's Space Product Development Program (SPD).

  11. Thermal Characterization of TPS Tiles

    SciTech Connect

    Kacmar, C. J.; LaCivita, K. J.; Jata, K. V.; Sathish, S.

    2006-03-06

    The Thermal Protection System (TPS) used on space shuttles protects the metallic structure from the large amounts of heat created during travel through the atmosphere, both on takeoff and reentry. The shuttle experiences high thermo-acoustic loading and impact damage from micro-meteorites, which can cause disbonds, delaminations, chips, cracks, and other defects to the TPS system. To enhance durability and damage tolerance, new TPS tiles with an added protective ceramic-matrix-composite layer are being developed. This paper explores the use of pulsed thermography as a quick, diverse, non-destructive technique, to characterize the TPS system. The pulsed thermography images obtained are presented and analyzed.

  12. NASA IKONOS Radiometric Characterization

    NASA Technical Reports Server (NTRS)

    Pagnutti, Mary; Frisbee, Troy; Zanoni, Vicki; Blonski, Slawek; Daehler, Erik; Grant, Brennan; Holekamp, Kara; Ryan, Robert; Sellers, Richard; Smith, Charles

    2002-01-01

    The objective of this program: Perform radiometric vicarious calibrations of IKQNOS imagery and compare with Space Imaging calibration coefficients The approach taken: utilize multiple well-characterized sites which are widely used by the NASA science community for radiometric characterization of airborne and spaceborne sensors; and to Perform independent characterizations with independent teams. Each team has slightly different measurement techniques and data processing methods.

  13. Adaptive Structures Flight Experiments

    NASA Technical Reports Server (NTRS)

    Martin, Maurice

    1992-01-01

    The topics are presented in viewgraph form and include the following: adaptive structures flight experiments; enhanced resolution using active vibration suppression; Advanced Controls Technology Experiment (ACTEX); ACTEX program status; ACTEX-2; ACTEX-2 program status; modular control patch; STRV-1b Cryocooler Vibration Suppression Experiment; STRV-1b program status; Precision Optical Bench Experiment (PROBE); Clementine Spacecraft Configuration; TECHSAT all-composite spacecraft; Inexpensive Structures and Materials Flight Experiment (INFLEX); and INFLEX program status.

  14. Adaptive structures flight experiments

    NASA Astrophysics Data System (ADS)

    Martin, Maurice

    The topics are presented in viewgraph form and include the following: adaptive structures flight experiments; enhanced resolution using active vibration suppression; Advanced Controls Technology Experiment (ACTEX); ACTEX program status; ACTEX-2; ACTEX-2 program status; modular control patch; STRV-1b Cryocooler Vibration Suppression Experiment; STRV-1b program status; Precision Optical Bench Experiment (PROBE); Clementine Spacecraft Configuration; TECHSAT all-composite spacecraft; Inexpensive Structures and Materials Flight Experiment (INFLEX); and INFLEX program status.

  15. NASA Work Experience

    NASA Technical Reports Server (NTRS)

    Frandsen, Athela F.

    2015-01-01

    I have had the opportunity to support the analytical laboratories in chemical analysis of unknown samples, using Optical Microscopy (OM), Polarizing Light Microscopy (PLM), Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy with Energy Dispersive Spectroscopy (SEMEDS), and X-ray Powder Diffraction (XPD). I have assisted in characterizing fibers pulled from a spacecraft, a white fibrous residue discovered in a jet refueler truck, brown residue from a plant habitat slated for delivery to the ISS (International Space Station), corrosion on a pipe from a sprinkler, and air filtration material brought back from the ISS. I also conducted my own fiber study in order to practice techniques and further my understanding of background concepts. Furthermore, I had the opportunity to participate in diverse work assignments, where I was assigned to work with other branches of the engineering department for 1-2 days each. The first was in the Materials Science branch where I participated in the construction of the plant habitat intended for use in research aboard the ISS. The second was in the Testing Design branch where I assisted with tensile and hardness testing of over 40 samples. In addition, I have had the privilege to attend multiple tours of the NASA KSC campus, including to the Astronaut Crew Quarters, the VAB (the main area, the Columbia room, and the catwalk), the Visitor Center housing the shuttle Atlantis, the Saturn-V exhibit, the Prototype laboratory, SWAMP WORKS, the Shuttle Landing Facility, the Crawler, and the Booster Fabrication Facility (BFF). Lastly, much of my coursework prepared me for this experience, including numerous laboratory courses with topics diverse as chemistry, physics, and biology.

  16. Synthesis and Electrochemistry of Cyclopentadienylcarbonyliron Tetramer: An Advanced Experiment.

    ERIC Educational Resources Information Center

    White, A. J.; Cunningham, Alice J.

    1980-01-01

    Describes an advanced level experiment in which a transition metal cluster compound, cyclopentadienylcarbonyliron tetramer, is synthesized and characterized spectroscopically. Its redox properties are then explored through cyclic voltammetry. (CS)

  17. Friedel-Crafts Acylation: An Experiment Incorporating Spectroscopic Structure Determination.

    ERIC Educational Resources Information Center

    Schatz, Paul F.

    1979-01-01

    Describes a laboratory experiment which demonstrates manipulation of highly reactive chemicals, use of a gas trap, and simple and reduced pressure distillation. Student must characterize starting material and product with nuclear magnetic resonance and infrared spectroscopy. (Author/SA)

  18. Silicic Volcanism Identified by the Diviner Lunar Radiometer Experiment

    NASA Astrophysics Data System (ADS)

    Glotch, T. D.; Greenhagen, B. T.; Hagerty, J. J.; Jolliff, B. L.; Ashley, J. W.; Williams, J.-P.; Petro, N. E.

    2016-05-01

    The Diviner Lunar Radiometer Experiment on the Lunar Reconnaissance Orbiter has mapped and characterized a number of silicic volcanic constructs on the lunar surface. Here, we summarize Diviner's contributions to our understanding of these features.

  19. Detector Development for the MARE Neutrino Experiment

    SciTech Connect

    Galeazzi, M.; Bogorin, D.; Molina, R.; Saab, T.; Ribeiro Gomes, M.

    2009-12-16

    The MARE experiment is designed to measure the mass of the neutrino with sub-eV sensitivity by measuring the beta decay of {sup 187}Re with cryogenic microcalorimeters. A preliminary analysis shows that, to achieve the necessary statistics, between 10,000 and 50,000 detectors are likely necessary. We have fabricated and characterized Iridium transition edge sensors with high reproducibility and uniformity for such a large scale experiment. We have also started a full scale simulation of the experimental setup for MARE, including thermalization in the absorber, detector response, and optimum filter analysis, to understand the issues related to reaching a sub-eV sensitivity and to optimize the design of the MARE experiment. We present our characterization of the Ir devices, including reproducibility, uniformity, and sensitivity, and we discuss the implementation and capabilities of our full scale simulation.

  20. Synthesis, Characterization, and Use of a Cobalt(II) Complex as an NMR Shift Reagent.

    ERIC Educational Resources Information Center

    Goff, Harold M.; And Others

    1982-01-01

    Describes procedures for preparing acetylacetonate complex of cobalt(II), followed by spectrophotometric analysis to characterize the material, with additional characterization methods supplied by students to provide open-ended dimension for the experiment. (SK)

  1. Preparation and Characterization of Myosin Proteins.

    ERIC Educational Resources Information Center

    Caldwell, Elizabeth; Eftink, Maurice R.

    1985-01-01

    Students complete five experimental projects at the end of a senior-level biochemistry course which involves the isolation and characterization of myosin and its water-soluble subfragments. Procedures used and results obtained are provided for such projects as viscosity and ATPase measurements and gel electrophoresis experiments. (JN)

  2. NORTH AMERICAN LANDSCAPE CHARACTERIZATION (NALC): RESEARCH PLAN

    EPA Science Inventory

    The North American Landscape Characterization (NALC) project is a component of the National Aeronautics and Space Administration (NASA) Landsat Pathfinder program of experiments to study global change issues. he NALC program is funded principally by the U.S. Environmental Protect...

  3. Characterization of Three LYSO Crystal Batches

    NASA Astrophysics Data System (ADS)

    Yang, Fan; Mao, Rihua; Zhang, Liyuan; Zhu, Ren-Yuan

    2015-02-01

    We report on three LYSO crystal batches characterized at the Caltech crystal laboratory for future HEP experiments: Twenty-five 20 cm long crystals for the SuperB experiment; twelve 13 cm long crystals for the Mu2e experiment and 623 14×14×1.5 mm plates with five holes for a LYSO/W Shashlik matrix for a beam test at Fermilab. Optical and scintillation properties measured are longitudinal Transmittance, light output and FWHM energy resolution. Correlations between these properties are also investigated.

  4. Characterization of three LYSO crystal batches

    NASA Astrophysics Data System (ADS)

    Yang, Fan; Mao, Rihua; Zhang, Liyuan; Zhu, Ren-Yuan

    2015-06-01

    We report on three LYSO crystal batches characterized at the Caltech crystal laboratory for future HEP experiments: 25 20 cm long crystals for the SuperB experiment; 12 13 cm long crystals for the Mu2e experiment and 623 14×14×1.5 mm3 plates with five holes for a LYSO/W Shashlik matrix for a beam test at Fermilab. Optical and scintillation properties measured are longitudinal transmittance, light output and FWHM energy resolution. Correlations between these properties are also investigated.

  5. Biomedical experiments. Part A: Biostack experiment

    NASA Technical Reports Server (NTRS)

    Buecker, H.; Horneck, G.; Reinholz, E.; Scheuermann, W.; Ruether, W.; Graul, E. H.; Planel, H.; Soleilhavoup, J. P.; Cuer, P.; Kaiser, R.

    1972-01-01

    The biostack experiment is described which was designed to study the biologic effects of individual heavy nuclei of galactic cosmic radiation during space flight outside the magnetosphere of the earth. Specifically, the biostack experiment was designed to promote research on the effects of high energy/high Z particles of galactic cosmic radiation on a broad spectrum of biologic systems, from the molecular to the highly organized and developed forms of life. The experiment was considered unique and scientifically meritorious because of its potential yield of information - currently unavailable on earth - on the interaction of biologic systems with the heavy particles of galactic cosmic radiation.

  6. Laboratory for Characterization of Irradiated Graphite

    SciTech Connect

    Karen A. Moore

    2010-03-01

    The newly completed Idaho National Laboratory (INL) Carbon Characterization Laboratory (CCL) is located in Labs C19 and C20 of the Idaho National Laboratory Research Center (IRC). The CCL was established under the Next Generation Nuclear Plant (NGNP) Project to support graphite and ceramic composite research and development activities. The research