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1

Parameters of haematology, clinical chemistry and lipid metabolism in the common marmoset and alterations under stress conditions.  

PubMed

Common marmosets are suitable non-human primate models for many human diseases. Standard values for blood parameters are required to evaluate physiological and pathological situations. Two studies were conducted: study I to determine standard values and study II to examine these under changed housing conditions. In study I, all parameters for clinical chemistry were similar in range for both genders with these specifics: male marmosets had significantly higher total and LDL cholesterol levels than females, whereas the mean corpuscular volume and the mean corpuscular haemoglobin were significantly lower than in females. In study II, glucose, lymphocytes and salivary cortisol were significantly lower, and faecal cortisol was increased during the change of housing conditions. In conclusion, standard values for haematology and clinical chemistry for the common marmoset were determined. Further on, parameters that are influenced by relocation stress and its importance for experimental results are described. PMID:22765494

Kuehnel, F; Grohmann, J; Buchwald, U; Koeller, G; Teupser, D; Einspanier, A

2012-07-06

2

Effects of fasting on haematology and clinical chemistry values in the rat and dog  

Microsoft Academic Search

Many regulatory toxicity guidelines and the recommendation of AACC-DACC\\/ASVCP joint task force of the USA on clinical pathology testing require overnight fasting for rats and non-rodents before blood sampling. However, the reason why animals must be fasted before blood sampling is unclear in toxicology studies.

T. Matsuzawa; M. Sakazume

1994-01-01

3

Normal and clinical haematology of captive cranes (gruiformes)  

Microsoft Academic Search

Fall blood counts on 56 clinically normal cranes of nine different species have provided reference values for the interpretation of haematological changes in 13 cranes presenting with abnormal clinical signs. Hypochromic anaemia, heterophilia and lymphocytosis were found in birds with probable Mycobacterium avium infection and heterophilia and lymphocytosis in birds with bumblefoot, arthritis, nephrosis and cardiac myo?pathy. In several of

Christine Hawkey; J. H. Samour; D. G. Ashton; M. G. Hart; R. N. Cindery; J. M. Ffinch; D. M. Jones

1983-01-01

4

Modifications of haematology analyzers to improve cell counting and leukocyte differentiating in cerebrospinal fluid controls of the Joint German Society for Clinical Chemistry and Laboratory Medicine.  

PubMed

Flow cytometry (FCM) is used with haematology analyzers (HAs) to count cells and differentiate leukocytes in cerebrospinal fluid (CSF). To evaluate the FCM techniques of HAs, 10 external DGKL trials with CSF controls were carried out in 2004 to 2008. Eight single platform HAs with and without CSF equipment were evaluated with living blood leukocytes and erythrocytes in CSF like DGKL controls: Coulter (LH750,755), Abbott CD3200, CD3500, CD3700, CD4000, Sapphire, ADVIA 120(R) CSF assay, and Sysmex XE-2100(R). Results were compared with visual counting of native cells in Fuchs-Rosenthal chamber, unstained, and absolute values of leukocyte differentiation, assayed by dual platform analysis with immune-FCM (FACSCalibur, CD45, CD14) and the chamber counts. Reference values X were compared with HA values Y by statistical evaluation with Passing/Bablock (P/B) linear regression analysis to reveal conformity of both methods. The HAs, studied, produced no valid results with DGKL CSF controls, because P/B regression revealed no conformity with the reference values due to:-blank problems with impedance analysis,-leukocyte loss with preanalytical erythrocyte lysis procedures, especially of monocytes,-inaccurate results with ADVIA cell sphering and cell differentiation with algorithms and enzyme activities (e.g., peroxidase). HA techniques have to be improved, e.g., using no erythrocyte lysis and CSF adequate techniques, to examine CSF samples precise and accurate. PMID:19526484

Kleine, Tilmann O; Nebe, C Thomas; Löwer, Christa; Lehmitz, Reinhard; Kruse, Rolf; Geilenkeuser, Wolf-Jochen; Dorn-Beineke, Alexandra

2009-08-01

5

Comparative haematological and plasma chemistry values in purpose-bred squirrel, cynomolgus and rhesus monkeys  

Microsoft Academic Search

Haematological and blood chemistry data have been compared for three species of purpose-bred primates, Macaca mulatta (rhesus monkey), Macaca fascicularis (cynomolgus monkey), Saimiri sciureus (squirrel monkey). These species were housed over a 9-month period in identical conditions (diet, tap water, room temperature and relative humidity, and lighting regime) in our primate facility. Blood and urine assays were conducted using the

T. Matsuzawa; Y. Nagai

1994-01-01

6

CLINICAL AND HAEMATOLOGICAL STUDIES IN DOGS, EXPERIMENTALLY INFECTED WITH TRICHURIS VULPIS  

Microsoft Academic Search

Summary Kirkova, Z., P. Petkov & D. Goundasheva, 2005. Clinical and haematological studies in dogs, experimentally infected with Trichuris vulpis. Bulg. J. Vet. Med., 8, No 2, 141?148. The experiment was performed on 9 mixed-breed dogs (6 infected and 3 non-infected controls). The experimental infection was provoked with Trichuris vulpis (10000 eggs\\/kg). The clinical signs and the following haematological parameters

Z. KIRKOVA; P. PETKOV; D. GOUNDASHEVA

2005-01-01

7

Haematology of clinically normal and abnormal captive llamas and guanacoes  

Microsoft Academic Search

Blood counts of healthy juvenile and adult llamas (Lama glama) and guanacoes (L guanacoe) showed that guanacoes have higher red cell counts, haemoglobin values and packed cell volumes than llamas. In both species, the numbers of lymphocytes and platelets were higher in juveniles than in adults. By reference to the values found in normal animals, abnormal haematological variations were detected

CM Hawkey; FM Gulland

1988-01-01

8

Clinical, ultrasonography and haematology of aglepristone-induced mid-gestation pregnancy terminations in rabbits.  

PubMed

Aglepristone is a safe abortifacient in cats, dogs and rabbits. Although no serious side effects have been reported, there is no information available about the effects of the medicine on haematological parameters. For the first time clinical and ultrasonographic features and haematological profiles were evaluated in rabbits treated with aglepristone 15 and 16 days after mating. Ten healthy 10-14 month-old New Zealand White female rabbits were mated with fertile bucks and pregnancies were con?rmed by ultrasound 15 days later. Of these, 5 does were treated with aglepristone (test group, n = 5) whilst the remaining five (control group, n = 5) were treated with a saline solution (0.9% NaCl). The treatment dose was 10 mg/kg body weight, administered subcutaneously once daily on two consecutive days (day 15 and 16 post mating). Ultrasonographic, clinical and haematological assessments were performed daily. Aglepristone treatment induced embryonic fluid resorptions without foetal death in mid-gestation terminations. Following ultrasonographic and haematological examinations, it was established that aglepristone is a safe abortifacient in rabbits. PMID:23718772

Özalp, Gözde R; Temizel, Ethem M; Özocak-Batmaz, Elçin

2013-05-06

9

American Association for Clinical Chemistry  

MedlinePLUS

American Association for Clinical Chemistry Better health through laboratory medicine Home | Customer Service | Site Map | Cart | Login About AACC Members Events Government & Public Affairs Resource ...

10

Clinical, haematological, biochemical and economic impacts of Trichinella spiralis infection in pigs.  

PubMed

The purpose of this work was to assess the clinical, haematological and biochemical responses of pigs experimentally inoculated with Trichinella spiralis. Groups of three pigs were inoculated per os with 100, 500 and 5000 T. spiralis muscle larvae, two pigs were used as control. Clinical evaluation of disease in pigs included daily examination, rectal temperature measurements and cardiac and respiration rates. Haematological studies included: hematocrit (%), hemoglobin (g/dl), and white cell, neutrophil, lymphocyte and eosinophil counts. Blood biochemistry included: bun (mg/dl), creatinine (mg/dl), AST (UI/l), ALT (UI/l), CPK (UI/l) and ALP (UI/l). No significant differences were observed in rectal temperature and in cardiac and respiration rates between inoculated animals and the control group (p> or =0.05). Significant differences were detected (p< or =0.05) in the values of % hemoglobin, and eosinophils, as well as in the values of CK, ALP, AST and ALT. The variations observed in some cases were related to the number of T. spiralis larvae inoculated and varied with the number of days post-infection. Inoculated pigs showed significant differences (p< or =0.05) in weight gain when compared with uninoculated controls. This study has clinical, haematological, and enzyme alterations in Trichinella infected pigs provides a better understanding of acute and chronic trichinellosis in pigs. PMID:17543458

Ribicich, M; Gamble, H R; Rosa, A; Sommerfelt, I; Marquez, A; Mira, G; Cardillo, N; Cattaneo, M L; Falzoni, E; Franco, A

2007-05-31

11

Clinical Chemistry and Clinical Toxicology Devices Panel ...  

Center for Biologics Evaluation and Research (CBER)

Text VersionClinical Chemistry and Clinical Toxicology Devices Panel Meeting April 25, 2013 Panel Discussion Questions Session III - Isoniazid Test Strips ... More results from www.fda.gov/downloads/advisorycommittees/committeesmeetingmaterials

12

Panel Roster Clinical Chemistry and Clinical Toxicology ...  

Center for Biologics Evaluation and Research (CBER)

Text Version... Clinical Chemistry and Clinical Toxicology Devices Panel Methotrexate Test Systems, Phencyclidine (PCP) Test Systems, Isoniazid Test Strips ... More results from www.fda.gov/downloads/advisorycommittees/committeesmeetingmaterials

13

Teaching Techniques in Clinical Chemistry.  

ERIC Educational Resources Information Center

|This master's thesis presents several instructional methods and techniques developed for each of eleven topics or subject areas in clinical chemistry: carbohydrate metabolism, lipid metabolism, diagnostic enzymology, endocrinology, toxicology, quality control, electrolytes, acid base balance, hepatic function, nonprotein nitrogenous compounds,…

Wilson, Diane

14

Clinical, haematological and therapeutic studies on tropical theileriosis in water buffaloes (Bubalus bubalis) in Egypt.  

PubMed

Thirty buffaloes naturally infected with Theileria annulata and 10 parasitologically free controls were used to determine the potential clinical, haematological and therapeutic impact of tropical theileriosis in Egypt. The clinical signs in the infected buffaloes were pyrexia (40.5-41.5 degrees C), enlargement of superficial lymph nodes, slight nasal and ocular discharges, salivation, anaemia and respiratory distress. Eye lesions also were recorded. There was a significant decrease in erythrocyte counts and haemoglobin content and a significant decrease in total leucocyte counts in infected buffaloes compared to controls. Early treatment with buparvaquone was 100% effective in eliminating the protozoan parasites from the blood and lymph nodes and led to an improvement in the clinical state whereas treatment in the later stages of the disease whilst eliminating the parasites failed to improve the clinical condition of the animal. PMID:17420101

Osman, Salama A; Al-Gaabary, Magdy H

2007-04-08

15

Capillary electrophoresis in clinical chemistry  

Microsoft Academic Search

Since its introduction, capillary electrophoresis has diversified, spreading out into different specialized fields covering solutions for almost any analytical questions arising in research laboratories. In the context of clinical chemistry, results must be provided at low costs and in a clinically relevent time frame; however, the attributes which have made capillary electrophoresis such a successful tool in basic research are

Rainer Lehmann; Wolfgang Voelter; Hartmut M. Liebich

1997-01-01

16

(Analytical instrumentation in clinical chemistry)  

SciTech Connect

As chairman of the Expert Panel on Instrumentation (EPI) of the International Federation of Clinical Chemistry (IFCC), I presided over its closed and open meetings held on June 29, 1987, and July 2, 1987, respectively. As chairman-elect of the Organizing Committee of the 1990 International Congress on Clinical Chemistry, I also attended the meeting of the IFCC Congress Committee to give a progress report. This report was subsequently also presented to the IFCC Executive Board and Council and to its corporate members. These meetings were held prior to, or in conjunction with, the 13th International Congress of Clinical Chemistry. Consequently, I had an opportunity to attend several of the scientific sessions and the Trade Exposition of the Congress.

Burtis, C.A.

1987-07-14

17

Teaching Techniques in Clinical Chemistry.  

ERIC Educational Resources Information Center

This master's thesis presents several instructional methods and techniques developed for each of eleven topics or subject areas in clinical chemistry: carbohydrate metabolism, lipid metabolism, diagnostic enzymology, endocrinology, toxicology, quality control, electrolytes, acid base balance, hepatic function, nonprotein nitrogenous compounds, and…

Wilson, Diane

18

Comparison of the clinical, microbiological, radiological and haematological features of foals with pneumonia caused by Rhodococcus equi and other bacteria  

Microsoft Academic Search

The purpose of this study was to compare the clinical, microbiological, radiological, haematological and cytological features of foals with pneumonia caused by Rhodococcus equi infection and with other bacteria, in order to provide markers for early diagnosis and treatment. A retrospective study of 113 cases of bacterial pneumonia was undertaken.Although there was considerable overlap in the affected populations, foals with

Mathilde Leclere; K. Gary Magdesian; Philip H. Kass; Nicola Pusterla; Diane M. Rhodes

2011-01-01

19

Sickle cell disease in Sierra Leone: a clinical and haematological analysis in older children and adults.  

PubMed

A prospective study of the clinical and haematological features of sickle cell disease in 121 Sierra Leonean patients (106 SS: sickle cell anaemia; 15 SC: sickle cell haemoglobin C disease) was undertaken. Fifty-five per cent of the patients were Creoles who were descendants of liberated slaves and who therefore had strong ethnic and genetic relationships with West Indians and black Americans. No essential differences were noted between the Creoles and non-Creole Sierra Leonean patients. The general clinical features and the patterns of vaso-occlusive crises were similar to those of black American, Jamaican and other West Africans (Ghanaians and Nigerians). However the incidence of certain complications such as avascular necrosis of the femoral head was higher in Sierra Leoneans (8.5% SS, 20% SC) than in other West Africans e.g. Ghanaians (2.8% SS, 6.6% SC) but similar to that of Jamaicans (8.2% SS, 18% SC). Also the frequency of leg ulceration in Sierra Leonean sickle cell patients (13.2% SS, 13.3% SC) was relatively low when compared to patients in the United States of America (73.6% SS) and Jamaica (63.3% SS) but relatively high in comparison to Nigerian (5.4% SS + SC + S beta-thal) and Ghanaian (10.6% SS, 2.2% SC) patients. The causes for such varying manifestations are probably environmental. The haematological features of the Sierra Leonean patients were similar to those of other population groups in all essential aspects. PMID:6639186

Knox-Macaulay, H H

1983-08-01

20

Optimising a curriculum for clinical haematology and biochemistry in sports medicine: a Delphi approach  

PubMed Central

Objectives To investigate issues of curriculum in the context of a postgraduate sports medicine training programme, specifically in the field of clinical biochemistry and haematology. Methods Following the Delphi methodology, a series of sequential questionnaires was administered to curriculum developers, clinical teachers, examiners, and registrars. Results Agreement on a core syllabus for this subject with an indication of the core aims and objectives of teaching and learning in this field and the associated required skills and competencies. An indication of current and ideal teaching and learning methods and reasons for these preferences. A consensus of key features of a teaching module for this field and of appropriate methods of examination. Conclusions The data derived from this study, as well as the experience of engaging in it, will better inform curriculum developers, teachers, and students of one another's perceptions as to what is important in and appropriate to teaching and learning in this field of sports medicine. Engagement in the exercise and broader consideration of the outcomes by those who develop the curriculum, teach, and formulate the examination process will facilitate attainment of the ideal of well aligned teaching, learning, and examination in this specific field.

Fallon, K E; Trevitt, A C

2006-01-01

21

Lead poisoning: clinical, biochemical, and haematological aspects of a recent outbreak.  

PubMed Central

The clinical, biochemical, and haematological aspects of a recent outbreak of lead poisoning, in which exposure was related to the oxyacetylene cutting of red lead painted ironwork, were investigated. Initial suspicion was raised when a blood film showed punctate basophilia which remains a simple and useful method of picking up lead toxicity. Estimations of blood lead concentration and conventional laboratory data confirmed the diagnosis. Although there was prominent punctate basophilia, spectrophotometric analysis showed only negligible accumulation of pyrimidine-5'-nucleotides despite severe suppression of pyrimidine-5'-nucleotidase activity. The pattern of the red cell glycolytic intermediates, investigated for the first time, suggested that lead may also affect glycolysis at the hexokinase step. Once the diagnosis was made intravenous chelation treatment was begun with a rapid improvement in symptoms. Long term follow up is required to assess any sequelae of intoxication. These cases emphasise the classic features of lead poisoning, and despite the currently available diagnostic tests, lead intoxication may still go unrecognised unless a thorough occupational history is taken. Images

Pagliuca, A; Mufti, G J; Baldwin, D; Lestas, A N; Wallis, R M; Bellingham, A J

1990-01-01

22

Roster of the Clinical Chemistry and Clinical Toxicology ...  

Center for Biologics Evaluation and Research (CBER)

... Roster of the Clinical Chemistry and Clinical Toxicology Devices Panel. Purpose: The Committee reviews and evaluates ... More results from www.fda.gov/advisorycommittees/committeesmeetingmaterials/medicaldevices

23

Evolution of clinical, haematological and biochemical findings in young dogs naturally infected by vector-borne pathogens.  

PubMed

Longitudinal studies evaluating the evolution of clinical, haematological, biochemical findings in young dogs exposed for the first time to multiple vector-borne pathogens have not been reported. With the objective of assessing the evolution of clinical, haematological and biochemical findings, these parameters were serially monitored in naturally infected dogs throughout a 1-year follow-up period. Young dogs, infected by vector-borne pathogens based on cytology or polymerase chain reaction, were examined clinically and blood samples were obtained at seven different follow-up time points. Dogs were randomized to group A (17 dogs treated with a spot-on formulation of imidacloprid 10% and permethrin 50%) or to group B (17 dogs untreated). In addition, 10 4-month-old beagles were enrolled in each group and used as sentinel dogs. At baseline, Anaplasma platys was the most frequently detected pathogen, followed by Babesia vogeli, Bartonella spp., Ehrlichia canis and Hepatozoon canis. Co-infections with A. platys and B. vogeli, followed by E. canis and B. vogeli, A. platys and H. canis and A. platys and Bartonella spp. were also diagnosed. In dogs from group B, abnormal clinical signs were recorded at different time points throughout the study. No abnormal clinical signs were recorded in group A dogs. Thrombocytopenia was the most frequent haematological alteration recorded in A. platys-infected dogs, B. vogeli-infected dogs and in dogs co-infected with A. platys and B. vogeli or A. platys and Bartonella spp. Lymphocytosis was frequently detected among dogs infected with B. vogeli or co-infected with A. platys and B. vogeli. Beagles were often infected with a single pathogen rather than with multiple canine vector-borne pathogens. There was a significant association (p<0.01) between tick infestation and A. platys or B. vogeli, as single infections, and A. platys and B. vogeli or A. platys and Bartonella spp. co-infections. This study emphasizes the clinical difficulties associated with assigning a specific clinical sign or haematological abnormality to a particular canine vector-borne disease. PMID:21106311

de Caprariis, Donato; Dantas-Torres, Filipe; Capelli, Gioia; Mencke, Norbert; Stanneck, Dorothee; Breitschwerdt, Edward B; Otranto, Domenico

2010-10-16

24

Haemoglobin Lepore Boston-Washington in Sicily: clinical, haematological, and biosynthetic studies.  

PubMed Central

In the south-east of Sicily 23 children from 14 unrelated families have been diagnosed as suffering from haemoglobin Lepore. Such a high incidence shows that Sicily is an important focus of haemoglobin Lepore. The results of haematological and biosynthetic studies in 18 carriers of Hb Lepore and in five double heterozygotes for Hb Lepore and beta-thalassaemia are presented. In the carriers the haematological and biosynthetic data are compared with carriers of beta-thalassaemia, while the five double heterozygotes are compared with beta o- and beta +-thalassaemia major subjects. In the carriers of Hb Lepore no synthesis of delta beta-chains was observed in peripheral blood cells; in fact we found a peak in the bone marrow. Double heterozygotes with circulating nucleated red cells showed delta beta-chain synthesis in peripheral blood.

Schiliro, G; Musumeci, S; Pizzarelli, G; Fischer, A; Romero, M A; Russo, G

1980-01-01

25

Brief Summary of the Clinical Chemistry and Clinical ...  

Center for Biologics Evaluation and Research (CBER)

Text Version... Public Health Service Brief Summary of the Clinical Chemistry and Clinical Toxicology Devices Panel Meeting – April 25, 2013 Introduction: ... More results from www.fda.gov/downloads/advisorycommittees/committeesmeetingmaterials

26

Continuing Education Instrumentation Training in Clinical Chemistry.  

ERIC Educational Resources Information Center

|Describes the continuing education program for clinical chemistry instrumentation training established at The College of Staten Island, New York. A course consisting of 14 sessions is outlined and discussed. (CS)|

LeBlanc, Jacqueline; Frankel, Saundra

1980-01-01

27

Clinical Chemistry: International Journal of Laboratory Medicine and Molecular Diagnostics  

NSDL National Science Digital Library

The American Association for Clinical Chemistry has recently made available online Clinical Chemistry: International Journal of Laboratory Medicine and Molecular Diagnostics. Clinical Chemistry contains full-text articles, figures, and tables in a searchable (by keyword) format. Coverage begins with the January 1998 issue. Abstracts begin with the January 1980 issue. Clinical Chemistry is made available through Stanford University's HighWire Press.

2007-05-17

28

Clinical Chemistry and Clinical Toxicology Devices Panel ...  

Center for Biologics Evaluation and Research (CBER)

... However, they felt that the tests should be used in conjunction with other traditional risk assessment tools and clinical judgment. ... More results from www.fda.gov/advisorycommittees/committeesmeetingmaterials/medicaldevices

29

New Techniques and Tools for Clinical Chemistry.  

National Technical Information Service (NTIS)

In this paper are described and evaluated several new tools of potential use in clinical chemistry. The first, intended to minimize required sample volumes, is a device with which a total sample volume of 1 micro liter can be dispensed in the form of 1000...

G. M. Hieftje

1983-01-01

30

Reference measurement systems in clinical chemistry  

Microsoft Academic Search

Background: In clinical chemistry, traceability of measurements is of high priority. Methods: In this literature review, current recommendations on the process of establishing traceability (or standardization) are critically discussed. Results: Traceability is to be established to the highest international standards by a comprehensive reference measurement system. Elementary to this system are a metrological basis, a measurement unit system, i.e., the

Linda M. Thienpont; Katleen Van Uytfanghe; André P. De Leenheer

2002-01-01

31

Trends and aspects in clinical chemistry.  

PubMed

This paper first seeks to evaluate current trends in methodology and technology and discusses possible implications for the future of clinical chemistry, whose range and diversity of tasks are carefully defined. It is thought that the remarkable development in the field of immunological methods, in the general sense of the work, will continue for the years to come. One other principle, which it is predicted will play an increasing role, is thermometry for the determination of enthalpy. Similarly, new developments in the fields of bio- and chemoluminescence offer interesting possibilities. The impact of the computer in clinical chemistry is also discused. Secondly, the double role of clinical chemistry as a diagnostic speciality and as an academic field is analyzed. On the one hand, the need for closer cooperation both with other diagnostic subspecialities and with other medical clinical disciplines is stressed. In particular, newly organized Departments of Laboratory Medicine encompassing microbiology, cytology, nuclear medicine, toxicology etc. might contribute greatly to interdisciplinary understanding. On the other hand, problems of clinical medicine and of certain areas within biological research should be at the center of interest of the future research-oriented clinical chemist. PMID:739238

Keller, H

1978-12-01

32

Clinical and haematological evaluation of beta thalassaemia intermedia characterised by unusually low Hb F and increased Hb A2: beta thalassaemia intermedia II.  

PubMed Central

A total of 15 patients from different families with thalassaemia intermedia was studied. Haematological studies showed that the fetal haemoglobin was only slightly raised, being between 2 and 11.5% of the total haemoglobin. Haemoglobin A2 was high in all cases. The family study indicated that homozygosity or compound heterozygosity for beta thalassaemia was present in five patients, while dominant inheritance was observed in three. In seven patients family studies were not sufficient to predict the genotype. Haematological findings in the parents of the homozygous patients were as severe as those seen in common Hb A2 beta thalassaemia traits. The decrease in MCH and MCV was more severe and the Hb A2 higher in homozygous patients than in cases of common beta thalassaemia major (p less than 0.01, p less than 0.01, and p less than 0.001 respectively). The imbalance in in vitro globin synthesis was more severe in classical beta thalassaemia major than in homozygous patients in this study (p less than 0.01). However, the imbalance in alpha/non-alpha synthetic ratios showed variation among the homozygous and heterozygous patients in this study (2.1 to 4.0). Haematological severity and Hb F value showed some slight variation among affected persons of the same family in the case of patients with severe beta thalassaemia heterozygosity. The G gamma/A gamma ratio of haemoglobin F was found to be close to that of the adult level. Haematological studies suggested that clinical and haematological findings were more severe in patients with homozygous beta thalassaemia than in patients with heterozygosity for beta thalassaemia. The prevalence of thalassaemia intermedia with low Hb F and increased Hb A2 was found to account for 3% of the Turkish beta thalassaemic patients diagnosed before the age of 8 years. Images

Gurgey, A; Kayin, S; Kansu, E; Altay, C

1985-01-01

33

Clinical chemistry of serotonin and metabolites  

Microsoft Academic Search

Analyses of serotonin and other 5-hydroxyindoles, such as its precursor 5-hydroxytryptophan and major metabolite 5-hydroxyindoleacetic acid (5-HIAA), are indispensable for the elucidation of their (patho)physiological roles. In clinical chemistry attention is mainly focused on the diagnosis and follow-up of carcinoid tumours. For this most laboratories routinely measure urinary 5-HIAA. More recently, measurements of serotonin in platelets and urine have been

Ido P Kema; Elisabeth G. E de Vries; Frits A. J Muskiet

2000-01-01

34

Growth, clinical chemistry and immune function in domestic piglets fed varying ratios of arachidonic acid and DHA.  

PubMed

In the USA, infant formulas contain long-chain PUFA arachidonic acid (ARA) and DHA in a ratio of 2:1 and comprise roughly 0·66 g/100 g and 0·33 g/100 g total fatty acids (FA). Higher levels of dietary DHA appear to provide some advantages in visual or cognitive performance. The present study evaluated the effect of physiologically high dietary ARA on growth, clinical chemistry, haematology and immune function when DHA is 1·0 g/100 g total FA. On day 3 of age, formula-reared (FR) piglets were matched for weight and assigned to one of six milk replacer formulas. Diets varied in the ratio of ARA:DHA as follows (g/100 g FA/FA): A1, 0·1/1·0; A2, 0·53/1·0; A3-D3, 0·69/1·0; A4, 1·1/1·0; D2, 0·67/0·62; D1, 0·66/0·33. A seventh group was maternal-reared (MR) and remained with the dam during the study. Blood collection and body weight measurements were performed weekly, and piglets were killed on day 28 of age. No significant differences were found among any of the FR groups for formula intake, growth, clinical chemistry, haematology or immune status measurements. A few differences in clinical chemistry, haematology and immune function parameters between the MR pigs and the FR groups probably reflected a difference in growth rate. We conclude that the dietary ARA level up to 1·0 g/100 g total FA is safe and has no adverse effect on any of the safety outcomes measured, and confirm that DHA has no adverse effect when ARA is at 0·66 g/100 g FA. PMID:22040360

Tyburczy, Cynthia; Kothapalli, Kumar S D; Park, Woo Jung; Blank, Bryant S; Liu, Ying-Chun; Nauroth, Julie M; Zimmer, J Paul; Salem, Norman; Brenna, J Thomas

2011-11-01

35

78 FR 13347 - Clinical Chemistry and Clinical Toxicology Devices Panel of the Medical Devices Advisory...  

Federal Register 2010, 2011, 2012, 2013

...Administration [Docket No. FDA-2013-N-0001] Clinical Chemistry and Clinical Toxicology Devices Panel of the Medical Devices...will be open to the public. Name of Committee: Clinical Chemistry and Clinical Toxicology Devices Panel of the Medical...

2013-02-27

36

Sports haematology.  

PubMed

While the crucial role of haemoglobin in aerobic exercise has been well accepted, there is still a great deal of controversy about the optimal haematological parameters in the athletic population. The initial part of this review will examine the question of anaemia in athletes. The most common finding in athletes is a dilutional pseudoanaemia that is caused by a plasma volume expansion, rather than an actual blood loss. It is not a pathological state and normalises with training cessation in 3 to 5 days. This entity should be distinguished from conditions associated with lowered blood counts, such as intravascular haemolysis or iron deficiency anaemia. The evaluation of true anaemia states in the athlete must take into account not only blood losses secondary to exercise, such as foot strike haemolysis or iron losses through sweat, but non-athletic causes as well. Depending on the age and sex of the athlete, consideration must be given to evaluation of the gastrointestinal or genitourinary systems for blood loss. Finally, a comprehensive nutritional history must be taken, as athletes, especially women, are frequently not consuming adequate dietary iron. The second section of the paper will deal with the very contentious issue of sickle cell trait. While there have been studies demonstrating an increased risk of sudden death in people with sickle cell trait, it is still quite rare and should not be used as a restriction to activity. Further, studies have demonstrated that patients with sickle cell trait have an exercise capacity that is probably normal or near normal. However, in the cases of sudden death, it has been secondary to rhabdomyolysis occurring among sickle cell trait athletes performing at intense exertion under hot conditions, soon after arriving at altitude. The recommendations are that athletes with sickle cell trait adhere to compliance with the general guidelines for fluid replacement and acclimatisation to hot conditions and altitude. The final section of the paper examines the issue of haematological manipulation for the purposes of ergogenic improvement. Although experiments with blood doping revealed improvements in running time to exhaustion and maximal oxygen uptake, the introduction of recombinant erythropoietin has rendered blood doping little more than a historical footnote. However, the improvements in performance are not without risk, and the use of exogenous erythropoietin has the potential for increased viscosity of the blood and thrombosis with potentially fatal results. Until a definitive test is developed for detection of exogenous erythropoietin, it will continue to be a part of elite athletics. PMID:10688281

Shaskey, D J; Green, G A

2000-01-01

37

Clinical, haematological and biochemical studies of babesiosis in native goats in Mosul  

Microsoft Academic Search

The study included examination of 175 native goats, 27 were infected with Babesia ovis , B. motasi , B. foliata and B. taylori , (recorded in Mosul for the first time) and 25 were clinically normal and served as control. Results indicated that the percentage of the infection with Babesiosis was 15.42% and the percentage of parasitemia ranged between 3.5-10.4%

E. G. Sulaiman; S. H. Arslan; Q. T. Al-Obaidi; E. Daham

38

Clinical presentation, haematological indices and management of children with severe and uncomplicated malaria in Douala, Cameroon.  

PubMed

This study carried out from January to June 2007, was undertaken to describe the clinical presentation of childhood malaria in Douala, a meso-endemic area as far as malaria transmission is concerned. One hundred and seventy eight children were enrolled after informed consent of their parents. The sample characteristics were recorded and clinical as well as preliminary laboratory investigations were performed. Thirty eight children coming for vaccination and counselling was targeted to serve as control. According to the results obtained, cerebral malaria (CM) seems to be associated with young age, whilst Malaria anaemia (MA) was predominant among older children. Hyperpyrexia and hyperparasitaemia were high among CM patients and 11.1% of them died, however, no neurological squeal was noticed immediately after discharge on those who survived. Haemoglobin and glycaemia were low on MA and CM patients; these groups had low percentage in bed nets utilization as well. These results suggest that the clinical presentation of the disease differ with the geographic location and malaria disease features varies according to the severity. Such studies could contribute to the management of the disease. PMID:19137849

Mfonkeu, J B Pankoui; Gouado, I; Kuate, H Fotso; Zambou, O; Grau, G; Combes, V; Zollo, P H Amvam

2008-10-15

39

Clinical, haematological and biochemical findings in milk-fed calves with chronic indigestion.  

PubMed

The principal clinical signs in 59 milk-fed calves with chronic indigestion were general malaise and depression, poor appetite, poor body condition, dehydration, a dull and scaly hair coat, alopecia and clay-like faeces. All the calves had metabolic acidosis, which was associated with an inability to stand up in more than half of them. There were significant differences in the severity of acidosis between the calves that could stand and those that could not. Other signs in some of the calves were dehydration, leucocytosis, and increased activities of liver enzymes. PMID:10515617

Stocker, H; Lutz, H; Rüsch, P

1999-09-11

40

Clinical chemistry since 1800: growth and development.  

PubMed

The 19th and 20th centuries witnessed the growth and development of clinical chemistry. Many of the individuals and the significance of their contributions are not very well known, especially to new members of the profession. This survey should help familiarize them with the names and significance of the contributions of physicians and chemists such as Fourcroy, Berzelius, Liebig, Prout, Bright, and Rees. Folin and Van Slyke are better known, and it was their work near the end of the second decade of the 20th century that brought the clinical chemist out of the annex of the mortuary and into close relationship with the patient at the bedside. However, the impact on clinical chemistry and the practice of medicine by the 1910 exposé written by Abraham Flexner is not as well known as it deserves to be, nor is the impetus that World War I gave to the spread of laboratory medicine generally known. In the closing decades of the 20th century, automated devices produced an overabundance, and an overuse and misuse, of testing to the detriment of careful history taking and bedside examination of the patient. This is attributable in part to a fascination with machine-produced data. There was also an increased awareness of the value of chemical methods of diagnosis and the need to bring clinician and clinical chemist into a closer partnership. Clinical chemists were urged to develop services into dynamic descriptions of the diagnostic values of laboratory results and to identify medical relevance in interpreting significance for the clinician. PMID:11751558

Rosenfeld, Louis

2002-01-01

41

21 CFR 862.2140 - Centrifugal chemistry analyzer for clinical use.  

Code of Federal Regulations, 2010 CFR

... 2010-04-01 false Centrifugal chemistry analyzer for clinical use. 862.2140...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical...Instruments § 862.2140 Centrifugal chemistry analyzer for clinical use. (a)...

2010-04-01

42

21 CFR 862.2160 - Discrete photometric chemistry analyzer for clinical use.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 false Discrete photometric chemistry analyzer for clinical use. 862.2160...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical... § 862.2160 Discrete photometric chemistry analyzer for clinical use. (a)...

2010-04-01

43

21 CFR 862.2150 - Continuous flow sequential multiple chemistry analyzer for clinical use.  

Code of Federal Regulations, 2010 CFR

... Continuous flow sequential multiple chemistry analyzer for clinical use. 862.2150...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical... Continuous flow sequential multiple chemistry analyzer for clinical use. (a)...

2009-04-01

44

21 CFR 862.2160 - Discrete photometric chemistry analyzer for clinical use.  

Code of Federal Regulations, 2010 CFR

...2009-04-01 false Discrete photometric chemistry analyzer for clinical use. 862.2160...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical... § 862.2160 Discrete photometric chemistry analyzer for clinical use. (a)...

2009-04-01

45

21 CFR 862.2160 - Discrete photometric chemistry analyzer for clinical use.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 false Discrete photometric chemistry analyzer for clinical use. 862.2160...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical... § 862.2160 Discrete photometric chemistry analyzer for clinical use. (a)...

2013-04-01

46

21 CFR 862.2140 - Centrifugal chemistry analyzer for clinical use.  

Code of Federal Regulations, 2013 CFR

... 2013-04-01 false Centrifugal chemistry analyzer for clinical use. 862.2140...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical...Instruments § 862.2140 Centrifugal chemistry analyzer for clinical use. (a)...

2013-04-01

47

21 CFR 862.2150 - Continuous flow sequential multiple chemistry analyzer for clinical use.  

Code of Federal Regulations, 2013 CFR

... Continuous flow sequential multiple chemistry analyzer for clinical use. 862.2150...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical... Continuous flow sequential multiple chemistry analyzer for clinical use. (a)...

2013-04-01

48

The chemistry of urinary stones around 1800: A first in clinical chemistry  

Microsoft Academic Search

The chemistry of urinary stones around 1800: A first in clinical chemistry. At the end of the 18th century, as soon as modern chemistry was created, dedicated physicians tried to apply it to medicine. A rewarding field was that of urinary lithiasis. Stones offered a sufficient amount of a relatively pure chemical present in the body. Indeed, urine and the

Gabriel Richet

1995-01-01

49

21 CFR 862.2150 - Continuous flow sequential multiple chemistry analyzer for clinical use.  

Code of Federal Regulations, 2010 CFR

...MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY...flow sequential multiple chemistry analyzer for clinical use...flow sequential multiple chemistry analyzer for clinical use is a modular analytical instrument...

2010-04-01

50

Sexual chemistry: Theoretical elaboration and clinical implications  

Microsoft Academic Search

Sexual chemistry is a readily recognized construct but one which has received little scientific or empirical attention. The present manuscript defines sexual chemistry as a nonspecific, subjective description of a mysterious, physical, emotional and sexual state that feels driven and mostly pleasurable existing in the context of an interpersonal relationship, a state that is synergistic in being a surprising product

Sandra Leiblum; Mike Brezsnyak

2006-01-01

51

The early days of atomic absorption spectrometry in clinical chemistry  

Microsoft Academic Search

An account is given of the first applications of atomic absorption spectrometry in clinical chemistry. These include the determination of calcium and magnesium in blood serum and of these elements, together with a range of heavy metals, in urine.

J. B Willis

1999-01-01

52

The early days of atomic absorption spectrometry in clinical chemistry  

NASA Astrophysics Data System (ADS)

An account is given of the first applications of atomic absorption spectrometry in clinical chemistry. These include the determination of calcium and magnesium in blood serum and of these elements, together with a range of heavy metals, in urine.

Willis, J. B.

1999-12-01

53

Contributions of Analytical Chemistry to the Clinical Laboratory.  

ERIC Educational Resources Information Center

|Highlights several analytical techniques that are being used in state-of-the-art clinical labs. Illustrates how other advances in instrumentation may contribute to clinical chemistry in the future. Topics include: biosensors, polarization spectroscopy, chemiluminescence, fluorescence, photothermal deflection, and chromatography in clinical

Skogerboe, Kristen J.

1988-01-01

54

Haematology and neurology  

Microsoft Academic Search

This review aims to update the reader on advances in the understanding of haematological conditions that may arise in neurological practice. Thrombophilia, antiphospholipid antibody syndrome, thrombotic thrombocytopenic purpura, sickle cell and clonal disorders associated with neuropathy are discussed.

Steven Austin; Hannah Cohen; Nick Losseff

2007-01-01

55

Quality Assessment of Interpretative Commenting in Clinical Chemistry  

Microsoft Academic Search

Background: Clinical interpretation of laboratory re- sults is an integral part of clinical chemistry. However, the performance goals for assessing interpretative com- menting in this discipline have not been as well estab- lished as for the quality of analytical requirements. Methods: We present a review of the 10 case reports circulated in the 2002 Patient Report Comments Pro- gram by

Ee Mun Lim; Ken A. Sikaris; Janice Gill; John Calleja; Peter E. Hickman; John Beilby; Samuel D. Vasikaran

56

78 FR 19717 - Clinical Chemistry and Clinical Toxicology Devices Panel of the Medical Devices Advisory...  

Federal Register 2010, 2011, 2012, 2013

...DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2013-N-0001] Clinical Chemistry and Clinical Toxicology Devices Panel of the Medical Devices Advisory Committee: Notice of Change of Meeting Schedule...

2013-04-02

57

AssayFinder--an online database for clinical chemistry assays.  

PubMed

AssayFinder is an electronic database of clinical chemistry assays. It currently holds 1300 entries from 63 laboratories. It was developed for hospital clinical chemistry laboratories that need to send patient samples to reference centres for complex analysis. AssayFinder anticipated use of the Internet for transmitting information between hospitals. It started as a disc containing individual hypertext markup language files. From the beginning AssayFinder was designed for Web browser software, usable even in laboratories without Internet access. Later, files were generated automatically from a database package, (FileMaker-Pro, Claris, Santa Clara, USA.). Direct on-line access to the database has now been achieved using the World Wide Web. In the future sites will submit new assays in electronic form. In addition, links to other relevant specialist web-sites will further increase its value to the clinical chemistry community. PMID:10023817

Falconer-Smith, J F

1998-12-01

58

21 CFR 862.2170 - Micro chemistry analyzer for clinical use.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Micro chemistry analyzer for clinical use. 862.2170...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical...Laboratory Instruments § 862.2170 Micro chemistry analyzer for clinical use. (a)...

2010-04-01

59

21 CFR 862.2170 - Micro chemistry analyzer for clinical use.  

Code of Federal Regulations, 2010 CFR

...2009-04-01 2009-04-01 false Micro chemistry analyzer for clinical use. 862.2170...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical...Laboratory Instruments § 862.2170 Micro chemistry analyzer for clinical use. (a)...

2009-04-01

60

21 CFR 862.2170 - Micro chemistry analyzer for clinical use.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 2013-04-01 false Micro chemistry analyzer for clinical use. 862.2170...CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical...Laboratory Instruments § 862.2170 Micro chemistry analyzer for clinical use. (a)...

2013-04-01

61

Liver in haematological disorders.  

PubMed

Prothrombotic haematological disorders, in particular myeloproliferative disorders, are identified in a significant proportion of patients with Budd-Chiari syndrome and portal vein thrombosis (PVT). Multiple prothrombotic disorders may coexist. PVT is diagnosed in one fourth of patients with cirrhosis and is more common with advanced liver disease and hepatocellular carcinoma. PVT in cirrhosis can precipitate decompensation. Intrahepatic microthrombosis may play a role in the pathogenesis of hepatic fibrosis. Sinusoidal obstruction syndrome is usually a complication of myeloablative treatment before haematopoietic stem cell transplantation. Post-transplant lymphoproliferative disorders can complicate liver transplantation and are related to Epstein-Barr virus infection. Hepatitis B reactivation in patients receiving chemotherapy for haematological malignancies is very common without pre-emptive treatment, and can lead to liver failure. Liver involvement is common in primary haematological diseases, such as haemolytic anaemias, lymphomas and leukaemia. PMID:24090939

Pieri, Giulia; Theocharidou, Eleni; Burroughs, Andrew K

2013-08-01

62

An international haematological survey  

PubMed Central

Haematological surveys of adult population samples were conducted simultaneously in 12 countries, all but one of which are in Europe. Haematological estimations on samples from nine of the countries were made in one central laboratory. Differences between countries in the mean levels of haemoglobin (and haematocrit and red cell count) were found to be relatively small, and the prevalence of levels below the arbitrary WHO levels for anaemia were, on the whole, low. In males, the evidence suggests a fall in haemoglobin level throughout adult life, which increases slightly in advanced age. In females, there is no evidence of any important relationship between age and haemoglobin level.

Elwood, P. C.; Hughes, J.; Abernethy, M.; Davies, R.; Gough, R.; Johnson, A. P.; Dubourg, A. Y.

1976-01-01

63

Impact of reference materials on accuracy in clinical chemistry  

Microsoft Academic Search

The analytical accuracy of the results of routine clinical chemistry measurements is contributed by a two-steps mechanism, involving transferring trueness from a higher metrological and monitoring the time-stability of trueness itself. In both operations, different materials are used: however, accuracy in the routine assay of genuine patient samples has to be the end product of this overall process. To such

Carlo Franzini; Ferruccio Ceriotti

1998-01-01

64

Specialized Gas Chromatography--Mass Spectrometry Systems for Clinical Chemistry.  

ERIC Educational Resources Information Center

|A discussion of the basic design and characteristics of gas chromatography-mass spectrometry systems used in clinical chemistry. A comparison of three specific systems: the Vitek Olfax IIA, Hewlett-Packard HP5992, and Du Pont DP-102 are included. (BB)|

Gochman, Nathan; And Others

1979-01-01

65

Strategies to promote rational clinical chemistry test utilization  

Microsoft Academic Search

Objective: To critically review the elements of laboratory services that result in inappropriate ordering of clinical chemistry tests and the efficacy of corrective interventions.Methods and Results: In our experience, inappropriate use of laboratory services derives from multiple factors, including the use of multitest profiles, organ- or disease-specific test panels, indiscriminate ordering, standing orders, excessive reporting delays, poor audit trails of

J. Thomas Hindmarsh; Andrew W. Lyon

1996-01-01

66

Clinical chemistry reference intervals for healthy elderly subjects13  

Microsoft Academic Search

In 1979, 304 healthy elderly individuals in New Mexico were recruited for a longitudinal study ofnutrition and aging. Repeat measurements on a yearly basis of commonly requested clinical chemistry analytes allowed the calculation of reference intervals, between and within-subject variance components, and percentiles for change in concentration between two yearly measurements. The latter was furtherdivided into analytical and biological variance

Philip J Garry; William C Hunt; Dorothy J VanderJagt; Robert L Rhyne

67

(Short overview of the Mexican Society of Clinical Chemistry meetings)  

SciTech Connect

Organized and chaired session on instrument evaluation at the XIV Congreso Nacional De Quimica Clinica which is the National Meeting of the Mexican Society of Clinical Chemistry. In addition, I presented a paper on calibration at a Congress workshop and spoke on the impact of technology in a symposium on quality control.

Burtis, C.

1991-01-01

68

Growth factors in haematological cancers.  

PubMed

Since their discovery just under a century ago, growth factors (GFs) have been used almost ubiquitously in haematology. Many haematological cancers are associated with bone marrow failure, either as a direct consequence of the disease or its treatment. Colony stimulating factors (CSFs) have been used to address the problems associated with the resulting cytopenias, however, concerns about the potential leukaemogenic effects of some of these CSFs led to a degree of initial hesitancy in usage, particularly in the management of acute myeloid leukaemia (AML). This has now been largely overcome. Other limitations have included cost and side effect profiles (the latter particularly with the multilineage factors). There has been wide variation locally, nationally and internationally in the usage of GFs. The American Society of Clinical Oncologists (ASCO) attempted to rationalise the usage of GFs by producing a consensus document enumerating the evidence-based indications for use of GFs. There is little information on cost effectiveness, this remains an important issue for the future. Peripheral blood stem cell transplantation (PBSCT) has revolutionised the management of many malignant conditions and has contributed to the increased use of growth factors. Many other indications are emerging for GFs used singly or in combination. Current clinical applications of GFs include: i) amelioration of cytopenias following chemotherapy and stem cell transplantation, ii) chemotherapy dose maintenance and escalation, iii) chemosensitisation and modification of disease states, iv) optimisation of methods for mobilisation of progenitor stem cells, v) immunotherapy, and vi) as therapeutic targets for treatment of haematolgical malignancies. PMID:15989543

Osuji, Nnenna; Pettengell, R

2002-05-01

69

THE EFFECT OF XYLAZINE AND XYLAZINE-ETORPHINE- ACEPROMAZINE COMBINATION ON SOME CLINICAL AND HAEMATOLOGICAL PARAMETERS IN IMPALA AND ELANDW  

Microsoft Academic Search

Impala (Aepyceros me\\/ampus) and eland (Taurotragus onyx) were im- mobilized with a xylazine-etorphine-acepromazine combination or with xylazine alone. Clinical observations were made and blood samples were taken at intervals to determine the drug effects on clinical and blood parameters. During the immobi- lization period heart rate and body temperature decreased, as well as numbers of circulating erythrocytes and leukocytes, and

S. DREVEMO; L. KARSTADLU

1974-01-01

70

Influence of a Regular, Standardized Meal on Clinical Chemistry Analytes  

PubMed Central

Background Preanalytical variability, including biological variability and patient preparation, is an important source of variability in laboratory testing. In this study, we assessed whether a regular light meal might bias the results of routine clinical chemistry testing. Methods We studied 17 healthy volunteers who consumed light meals containing a standardized amount of carbohydrates, proteins, and lipids. We collected blood for routine clinical chemistry tests before the meal and 1, 2, and 4 hr thereafter. Results One hour after the meal, triglycerides (TG), albumin (ALB), uric acid (UA), phosphatase (ALP), Ca, Fe, and Na levels significantly increased, whereas blood urea nitrogen (BUN) and P levels decreased. TG, ALB, Ca, Na, P, and total protein (TP) levels varied significantly. Two hours after the meal, TG, ALB, Ca, Fe, and Na levels remained significantly high, whereas BUN, P, UA, and total bilirubin (BT) levels decreased. Clinically significant variations were recorded for TG, ALB, ALT, Ca, Fe, Na, P, BT, and direct bilirubin (BD) levels. Four hours after the meal, TG, ALB, Ca, Fe, Na, lactate dehydrogenase (LDH), P, Mg, and K levels significantly increased, whereas UA and BT levels decreased. Clinically significant variations were observed for TG, ALB, ALT, Ca, Na, Mg, K, C-reactive protein (CRP), AST, UA, and BT levels. Conclusions A significant variation in the clinical chemistry parameters after a regular meal shows that fasting time needs to be carefully considered when performing tests to prevent spurious results and reduce laboratory errors, especially in an emergency setting.

Salvagno, Gian Luca; Lippi, Giuseppe; Gelati, Matteo; Montagnana, Martina; Danese, Elisa; Picheth, Geraldo; Guidi, Gian Cesare

2012-01-01

71

A comparative study of clinical manifestations, haematological and serological responses after experimental infection with Anaplasma phagocytophilum in two Norwegian sheep breeds  

PubMed Central

Background It has been questioned if the old native Norwegian sheep breed, Old Norse Sheep (also called Norwegian Feral Sheep), normally distributed on coastal areas where ticks are abundant, is more protected against tick-borne infections than other Norwegian breeds due to a continuously high selection pressure on pasture. The aim of the present study was to test this hypothesis in an experimental infection study. Methods Five-months-old lambs of two Norwegian sheep breeds, Norwegian White (NW) sheep and Old Norse (ON) sheep, were experimentally infected with a 16S rRNA genetic variant of Anaplasma phagocytophilum (similar to GenBank accession number M73220). The experiment was repeated for two subsequent years, 2008 and 2009, with the use of 16 lambs of each breed annually. Ten lambs of each breed were inoculated intravenously each year with 0.4 ml A. phagocytophilum-infected blood containing approximately 0.5 × 106 infected neutrophils/ml. Six lambs of each breed were used as uninfected controls. Half of the primary inoculated lambs in each breed were re-challenged with the same infectious dose at nine (2008) and twelve (2009) weeks after the first challenge. The clinical, haematological and serological responses to A. phagocytophilum infection were compared in the two sheep breeds. Results The present study indicates a difference in fever response and infection rate between breeds of Norwegian sheep after experimental infection with A. phagocytophilum. Conclusion Although clinical response seems to be less in ON-lambs compared to NW-lambs, further studies including more animals are needed to evaluate if the ON-breed is more protected against tick-borne infections than other Norwegian breeds.

2011-01-01

72

Colloidal oatmeal: history, chemistry and clinical properties.  

PubMed

Oatmeal has been used for centuries as a soothing agent to relieve itch and irritation associated with various xerotic dermatoses. In 1945, a ready to use colloidal oatmeal, produced by finely grinding the oat and boiling it to extract the colloidal material, became available. Today, colloidal oatmeal is available in various dosage forms from powders for the bath to shampoos, shaving gels, and moisturizing creams. Currently, the use of colloidal oatmeal as a skin protectant is regulated by the U.S. Food and Drug Administration (FDA) according to the Over-The-Counter Final Monograph for Skin Protectant Drug Products issued in June 2003. Its preparation is also standardized by the United States Pharmacopeia. The many clinical properties of colloidal oatmeal derive from its chemical polymorphism. The high concentration in starches and beta-glucan is responsible for the protective and water-holding functions of oat. The presence of different types of phenols confers antioxidant and anti-inflammatory activity. Some of the oat phenols are also strong ultraviolet absorbers. The cleansing activity of oat is mostly due to saponins. Its many functional properties make colloidal oatmeal a cleanser, moisturizer, buffer, as well as a soothing and protective anti-inflammatory agent. PMID:17373175

Kurtz, Ellen S; Wallo, Warren

2007-02-01

73

Repeated transfusions of autologous cytokine-induced killer cells for treatment of haematological malignancies in elderly patients: a pilot clinical trial.  

PubMed

The elderly population is susceptible to haematological malignancies, and these elderly patients are intolerant to cytotoxic drugs. Therefore, the exploration of a safe and reliable strategy exclusive of chemotherapy is critical in improving the prognosis of elderly patients with haematological malignancies. We evaluated the safety and the efficacy of autologous cytokine-induced killer (CIK) cells combined with recombinant human interleukin 2 (rhIL-2) in the treatment of haematological malignancies in elderly patients. Peripheral blood mononuclear cells were isolated from 20 elderly patients with haematological malignancies, then augmented by priming with interferon gamma, rhIL-2 and CD3 monoclonal antibody. The autologous CIK cells (2-3 × 10(9)) were transfused back to patients, followed by a subcutaneous injection of IL-2 (1 mU/day) for 10 consecutive days. The regimen was repeated every 4 weeks. The host cellular immune function, tumour-related biological parameters, imaging characteristics, disease condition, quality of life and survival time were assessed. Fourteen patients received 8 cycles of transfusion and 6 received 4 cycles. No adverse effects were observed. The percentages of CD3(+), CD3(+) CD8(+) and CD3(+) CD56(+) cells were significantly increased (p < 0.05), and the levels of serum ?2 microglobulin and lactate dehydrogenase (LDH) were markedly decreased (p < 0.05) after autologous CIK cell transfusion. Cancer-related symptoms were profoundly alleviated, as demonstrated by the improved quality of life (p < 0.01). Complete remission was observed in 11 patients, persistent partial remission in 7 patients and stable disease in 2 patients. At the end of follow-up, the mean survival time was 20 months. Transfusion with autologous CIK cells plus rhIL-2 treatment is safe and effective for treating haematological malignancies in elderly patients. PMID:22972689

Yang, Bo; Lu, Xue-chun; Yu, Rui-li; Chi, Xiao-hua; Liu, Yang; Wang, Yao; Dai, Han-ren; Zhu, Hong-li; Cai, Li-li; Han, Wei-dong

2011-08-23

74

Mucosal barrier injury: biology, pathology, clinical counterparts and consequences of intensive treatment for haematological malignancy: an overview.  

PubMed

Mucositis is an inevitable side-effect of the conditioning regimens used for haematopoietic stem cell transplantation. The condition is better referred to as mucosal barrier injury (MBI) since it is primarily the result of toxicity and is a complex and dynamic pathobiological process manifested not only in the mouth but also throughout the entire digestive tract. A model has been proposed for oral MBI and consists of four phases, namely inflammatory, epithelial, ulcerative and healing phases. A variety of factors are involved in causing and modulating MBI including the nature of the conditioning regimen, the elaboration of pro-inflammatory and other cytokines, translocation of the resident microflora and their products, for example, endotoxins across the mucosal barrier, exposure to antimicrobial agents and whether or not the haematopoietic stem cell graft is from a donor. Neutropenic typhlitis is the most severe gastrointestinal manifestation of MBI, but it also influences the occurrence of other major transplant-related complications including acute GVHD, veno-occlusive disease and systemic infections. The pathobiology, clinical counterparts and the means of measuring MBI are discussed together with potential approaches for prevention, amelioration and, perhaps, even cure. Bone Marrow Transplantation (2000) 25, 1269-1278. PMID:10871732

Blijlevens, N M; Donnelly, J P; De Pauw, B E

2000-06-01

75

Development and diffusion of automated clinical chemistry analyzers  

NASA Astrophysics Data System (ADS)

The development and diffusion of automated clinical chemistry analyzers are described as an example of the process of technological change in medicine. This involves an elaboration and refinement of the conceptual framework, a historical description of development and use of three classes of analyzer, an investigation of user innovation, and the use of survey data to study patterns of diffusion. The emerging theory of development and diffusion differs from traditional theory in significant respects, assigning a more prominent and active role to the user in development, and characterizing development and diffusion as incremental and iterative rather than discrete and sequential.

Marks, H.; Fineberg, H. V.

1984-08-01

76

Automated haematology analysis to diagnose malaria  

PubMed Central

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ? 95% in samples with > 100 parasites/?l. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the method is applied. Future developments in new haematology analysers such as considerably simplified, robust and inexpensive devices for malaria detection fitted with an automatically generated alert could improve the detection capacity of these instruments and potentially expand their clinical utility in malaria diagnosis.

2010-01-01

77

Automated haematology analysis to diagnose malaria.  

PubMed

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ? 95% in samples with > 100 parasites/?l. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the method is applied. Future developments in new haematology analysers such as considerably simplified, robust and inexpensive devices for malaria detection fitted with an automatically generated alert could improve the detection capacity of these instruments and potentially expand their clinical utility in malaria diagnosis. PMID:21118557

Campuzano-Zuluaga, Germán; Hänscheid, Thomas; Grobusch, Martin P

2010-11-30

78

National Cancer Institute and American Association for Clinical Chemistry Partner to Bridge the Gap  

Cancer.gov

The National Cancer Institute, through its Clinical Proteomic Technologies for Cancer (CPTC) initiative has entered into a memorandum of understanding with the American Association for Clinical Chemistry (AACC) to join forces to promote and educate the clinical chemistry community in the area of proteomic standards and technology advances.

79

Time series modeling for quality control in clinical chemistry.  

PubMed

Autocorrelation of clinical chemistry quality-control (Q/C) measurements causes one of the basic assumptions underlying the use of Levey-Jennings control charts to be violated and performance to be degraded. This is the requirement that the observations be statistically independent. We present a proposal for a new approach to statistical quality control that removes this difficulty. We propose to replace the current single control chart of raw Q/C data with two charts: (a) a common cause chart, representing a Box-Jenkins ARIMA time-series model of any underlying persisting nonrandomness in the process, and (b) a special cause chart of the residuals from the above model, which, being free of such persisting nonrandomness, fulfills the criteria for use of the standard Levey-Jennings plotting format and standard control rules. We provide a comparison of the performance of our proposed approach with that of current practice. PMID:3390910

Alwan, L C; Bissell, M G

1988-07-01

80

CYP17A1: a biochemistry, chemistry, and clinical review.  

PubMed

Cytochrome P450 17A1 (CYP17A1; also P450c17and P450sccII) is a critically important enzyme in humans that catalyzes the formation of all endogenous androgens. It is an atypical cytochrome P450 enzyme in that it catalyzes two distinct types of substrate oxidation. Through its hydroxylase activity, it catalyzes the 17?-hydroxylation of pregnenolone to 17?-OH pregnenolone. Subsequently, through its C17,20lyase activity, it can further convert 17?-OH pregnenolone to the androgen dehydroepiandrosterone, which is a precursor to androstenedione, testosterone, and dihydrotestosterone. The importance of androgens in diseases such as prostate cancer has been appreciated for decades and the discovery of extra-testicular formation of androgens has helped clarify the pathology of the disease, especially the castrate- resistant disease. Therefore, specific inhibition of CYP17A1 by therapeutic intervention has been an area of considerable effort in several research laboratories. This basic research has led to the discovery of several promising drug candidates followed by the conduct of several clinical trials. Recently, all these efforts have culminated in the first approval by FDA of an inhibitor of CYP17A1 for the treatment of castrate-resistant prostate cancer. Ongoing clinical trials are now evaluating the agent in earlier stages of prostate cancer and even rare forms of androgen-dependent breast cancer. Accordingly, this review focuses on the biochemistry, chemistry, and clinical inhibitors of CYP17A1. PMID:23688130

Porubek, David

2013-01-01

81

Malaria: a haematological disease.  

PubMed

Plasmodium falciparum malaria remains a major cause of mortality throughout the tropical world. Haematological abnormalities are considered a hallmark of malaria, bearing an impact on final outcome and representing indices of prognostic and follow-up value. These include severe anaemia, coagulation disturbances, leukocyte numerical or functional changes and spleen involvement. Anaemia involves red blood cell lysis due to parasite invasion, as well as mechanisms of intravascular haemolysis and decreased erythropoiesis. Exchange or blood transfusion is mainly recommended in the management of these patients. Haemorrhagic complications in severe malaria are relatively rare despite prominent thrombocytopenia and dysfunction in the coagulation pathway. Numerical, as well as functional changes in the white blood cell are less dramatic than other blood cell series, but still, remain a significant index of disease progression and ultimate prognosis. Finally, the role of the spleen in severe malaria is multifactorial. Care and vigilance should be taken against splenic rupture which is fatal and can occur despite appropriate antimalarial prophylaxis and treatment. PMID:22664049

Akinosoglou, Karolina S; Solomou, Elena E; Gogos, Charalambos A

2012-03-01

82

Chemistry  

NSDL National Science Digital Library

Finds ChemEd DL resources related to the sections of the General Chemistry textbook, Chemistry, by Kenneth W. Whitten, Raymond E. Davis, M. Larry Peck, George G. Stanley published by Brooks/Cole, 2010.

83

Spleen in haematological malignancies: spectrum of imaging findings  

PubMed Central

Imaging morphology and metabolic activity of splenic lesions is of paramount importance in patients with haematological malignancies; it can alter tumour staging, treatment protocols and overall prognosis. CT, MRI and positron emission tomography (PET)/CT have been shown to be powerful tools for the non-invasive assessment of splenic involvement in various haematological malignancies. Since many haematological malignancies and non-neoplastic conditions can involve the spleen and imaging manifestations can overlap, imaging and clinical findings outside of the spleen should be looked for to narrow the differential diagnosis; confirmation can be obtained by pathological findings. Radiologists should be familiar with the cross-sectional imaging patterns of haematological malignancies involving the spleen as well as non-neoplastic splenic findings common in these patients to facilitate their care and follow-up. This pictorial review provides the common and uncommon imaging appearances and complications of various haematological malignancies involving the spleen on CT, MRI and PET/CT, and common pitfalls in diagnosis.

Saboo, S S; Krajewski, K M; O'Regan, K N; Giardino, A; Brown, J R; Ramaiya, N; Jagannathan, J P

2012-01-01

84

A Bridge between Two Cultures: Uncovering the Chemistry Concepts Relevant to the Nursing Clinical Practice  

ERIC Educational Resources Information Center

|This study focused on the undergraduate course that covers basic topics in general, organic, and biological (GOB) chemistry at a mid-sized state university in the western United States. The central objective of the research was to identify the main topics of GOB chemistry relevant to the clinical practice of nursing. The collection of data was…

Brown, Corina E.; Henry, Melissa L. M.; Barbera, Jack; Hyslop, Richard M.

2012-01-01

85

Medical Chemists and the Origins of Clinical Chemistry in Britain (circa 1750 -1850)  

Microsoft Academic Search

In this history, I review developments leading toward the establishment of clinical chemistry in Britain. Chemical research by certain physicians occurred in the context of medical traditions founded on vitalism, dis- tillation analysis, and limited chemical knowledge. Urine chemistry figured prominently in this period together with the analysis of kidney and bladder stones. Bright's team studying albuminuria was the first

Noel G. Coley

86

EFFECT OF CYPERMETHRIN ON CLINICO-HAEMATOLOGICAL PARAMETERS IN RABBITS  

Microsoft Academic Search

The aim of this study was to know the clinical and haematological changes in female rabbits following the treatment with cypermethrin (CY). In this study, 24 female rabbits were divided randomly into four equal groups A, B, C and D. Groups A, B and C were injected intraperitoneally CY @ 25, 50 and 75 mg\\/Kg b. wt., respectively, while the

M. KAMAL SHAH; A. KHAN; F. RIZVI; M. SIDDIQUE

87

A Preliminary Clinical Chemistry Laboratory Quality Control Programme for Addis Ababa.  

National Technical Information Service (NTIS)

A serum pool programme in clinical chemistry was started in Addis Ababa as a control for 5 participating laboratories. Basic statistical indices were calculated to determine the variation within each laboratory and to compare interlaboratory results. Stat...

C. P. Fulass R. Gillum

1973-01-01

88

Haematological disorders in Down syndrome  

Microsoft Academic Search

Haematological abnormalities are common in children with Down syndrome. These are mostly benign. Neonatal changes include polycythaemia, neutrophilia and thrombocytopenia. In later childhood changes include secondary polycythaemia, red-cell macrocytosis, increased red-cell distribution width, leukopenia, and immune dysfunction. Transient myeloproliferative disorder occurs in about 5% and indicates a group at particularly high risk of subsequent leukaemia. A full blood count should

Rebecca James; Sally Kinsey

2009-01-01

89

Matrix-assisted laser desorption\\/ionization time-of-flight mass spectrometry in clinical chemistry  

Microsoft Academic Search

Matrix-assisted laser desorption\\/ionization time-of-flight mass spectrometry (MALDI-Tof-MS) has recently become a popular and versatile method to analyze macromolecules from biological origin. In this paper, we will review the application of MALDI-Tof-MS in clinical chemistry and biology. MALDI-Tof-MS is used in clinical chemistry, e.g. disease markers can be identified with MALDI-MS analysis in combination with 1-D and 2-D gel electrophoresis separations

Laure F. Marvin; Matthew A. Roberts; Laurent B. Fay

2003-01-01

90

The Potential Role of Aurora Kinase Inhibitors in Haematological Malignancies  

PubMed Central

Summary Aurora kinases play an important role in the control of the cell cycle and have been implicated in tumourigenesis in a number of cancers. Among the haematological malignancies, overexpression of Aurora kinases has been reported in acute myeloid leukaemia, chronic myeloid leukaemia, acute lymphoblastic leukaemia, multiple myeloma, aggressive non-Hodgkin lymphoma and Hodgkin lymphoma. A large number of Aurora kinase inhibitors are currently in different stages of clinical development. In addition to varying in their selectivity for the different Aurora kinases, some also have activity directed at other cellular kinases involved in important molecular pathways in cancer cells. This review summarizes the biology of Aurora kinases and discusses why they may be good therapeutic targets in different haematological cancers. We describe preclinical data that has served as the rationale for investigating Aurora kinase inhibitors in different haematological malignancies, and summarize published results from early phase clinical trials. While the anti-tumour effects of Aurora kinase inhibitors appear promising, we highlight important issues for future clinical research and suggest that the optimal use of these inhibitors is likely to be in combination with cytotoxic agents already in use for the treatment of various haematological cancers.

Farag, Sherif S.

2011-01-01

91

Interferon-alpha in childhood haematological malignancies.  

PubMed Central

The application of cytostatics has brought about a breakthrough in the treatment of childhood haematological malignancies in the past 20 years. Chemotherapy appears to be least successful in the rare, low and very high mitotic index diseases, which often have an enormous tumour-burden. The suitability of chemotherapy in minimal residual leukaemia is also of some doubt. In these situations a 'conservative' treatment may be more appropriate. Because interferon-alpha has a distinct mechanism of action, and a broad-spectrum haematopoietic inhibitory activity, it is relatively nontoxic and noncancerogenic, and it may have a role in the treatment of malignant haematological disorders, either as a mono- or combination therapy. The exact indications and dosages for interferon in childhood malignancies are far from clear. Up to now, it has proved to be most efficacious in small tumour masses, providing a theoretical basis for application in minimal residual disease. Controlled clinical data, however, are not yet available. It remains to be determined whether or not interferon can be added to current chemotherapy protocols without a significant reduction of dose. Hopefully, a deeper understanding of the activities of interferon will allow us to plan better trials with combination treatments.

Simko, R.; Nagy, K.

1996-01-01

92

Chemistry  

NSDL National Science Digital Library

Chemistry is the scientific study of matter and its interaction with other matter and with energy. It is the branch of natural science that deals with the composition of substances and their properties and reactions.

K-12 Outreach,

93

Current and future use of "dried blood spot" analyses in clinical chemistry.  

PubMed

Abstract The analysis of blood spotted and dried on a matrix (i.e., "dried blood spot" or DBS) has been used since the 1960s in clinical chemistry; mostly for neonatal screening. Since then, many clinical analytes, including nucleic acids, small molecules and lipids, have been successfully measured using DBS. Although this pre-analytical approach represents an interesting alternative to classical venous blood sampling, its routine use is limited. Here, we review the application of DBS technology in clinical chemistry, and evaluate its future role supported by new analytical methods such as mass spectrometry. PMID:23740687

Lehmann, Sylvain; Delaby, Constance; Vialaret, Jérôme; Ducos, Jacques; Hirtz, Christophe

2013-10-01

94

An Enzymatic Clinical Chemistry Laboratory Experiment Incorporating an Introduction to Mathematical Method Comparison Techniques  

ERIC Educational Resources Information Center

|An enzymatic laboratory experiment based on the analysis of serum is described that is suitable for students of clinical chemistry. The experiment incorporates an introduction to mathematical method-comparison techniques in which three different clinical glucose analysis methods are compared using linear regression and Bland-Altman difference…

Duxbury, Mark

2004-01-01

95

Toluidine blue: A review of its chemistry and clinical utility  

PubMed Central

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property. Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage. This article provides an overview on chemistry, technique, and the various applications of toluidine blue.

Sridharan, Gokul; Shankar, Akhil A

2012-01-01

96

The future of clinical chemistry and its role in healthcare: a report of the Athena Society.  

PubMed

Given the omnipresent cost-containment environment in which clinical chemists now work, they must adapt to a host of changed conditions and new pressures. Much of the onus of adapting is on the individual who must assume a different attitude to his or her work. The American Association for Clinical Chemistry can, and should, take a leadership role in developing a new type of laboratory director by working with other professional organizations in the clinical laboratory field to create training programs and retraining programs for existing clinical laboratory scientists, which will equip them for broader scientific and managerial responsibilities than hitherto. AACC needs to develop alliances with its sister organizations so that the common issues are addressed collectively rather than competitively. The scope of clinical chemistry must expand into areas other than traditional clinical chemistry, e.g., microbiology, immunology, certain aspects of hematology (including coagulation), and even aspects of blood banking. The former clinical chemist needs to become a clinical laboratory scientist and promote him- or herself as having cross-disciplinary expertise in analytical techniques and automation, which are the common threads linking all branches of clinical laboratory science. PMID:8565242

1996-01-01

97

ROC Curves in Clinical Chemistry: Uses, Misuses, and Possible Solutions  

Microsoft Academic Search

Background: ROC curves have become the standard for describing and comparing the accuracy of diagnostic tests. Not surprisingly, ROC curves are used often by clinical chemists. Our aims were to observe how the accuracy of clinical laboratory diagnostic tests are as- sessed, compared, and reported in the literature; to identify common problems with the use of ROC curves; and to

Nancy A. Obuchowski; Michael L. Lieber; Frank H. Wians

2004-01-01

98

Matrix-assisted laser desorption\\/ionization time-of-flight mass spectrometry in clinical chemistry  

Microsoft Academic Search

Abstract Matrix-assisted laser desorption\\/ionization time-of-flight mass,spectrometry,(MALDI-Tof-MS) has recently become,a popular and versatile method to analyze macromolecules from biological origin. In this paper, we will review the application of MALDI-Tof-MS in clinical chemistry and biology. MALDI-Tof-MS is used in clinical chemistry, e.g. disease markers can be identified with MALDI-MS analysis in combination,with 1-D and 2-D gel electrophoresis separations thanks to either

Laure F. Marvin; Matthew A. Roberts; Laurent B. Fay

99

HAEMATOLOGY OF THE WOOD DUCK, Chenonetta jubata  

Microsoft Academic Search

Haematologic values have been determined for the wood duck, Chenonetta jubata, captured in the western fringes of Sydney. Values for females were usually slightly higher, and this follows the pattern for other species sampled in the pre- nesting period. The differences in haematological values between males and females were not statistically significant.

R. C. MULLEY

1980-01-01

100

Projecting the impact of the 1976 Medical Device Amendments on innovation in clinical chemistry tests  

Microsoft Academic Search

This paper is concerned with the impact of the Medical Device Amendments of 1976 and its implications in terms of innovation. With this objective the paper first describes the Medical Device Amendments of 1976. The paper then reports empirical evidence on the sources of a subset of innovations regulated by the Amendment's clinical chemistry diagnostic products. Finally, based on the

Stan N. Finkelstein; Eric von. Hippel; Jeffrey R. Scott

1980-01-01

101

50th anniversary of Clinical Chemistry and Laboratory Medicine--a historical overview.  

PubMed

In the early 1960s, Joachim Brugsch, one of the founders of Clinical Chemistry and Laboratory Medicine (CCLM) (then Zeitschrift für Klinische Chemie), had the idea to found a journal in the upcoming field of clinical chemistry. He approached Ernst Schütte, who was associated with the De Gruyter publishing house through another journal, to participate, and Schütte thus became the second founder of this Journal. The aim was to create a vehicle allowing the experts to express their opinions and raise their voices more clearly than they could in a journal that publishes only original experimental papers, a laborious and difficult, but important endeavor, as the profession of clinical chemistry was still in the early stages of development at this time. The first issue of this Journal was published in early 1963, and today, we are proud to celebrate the 50th anniversary of CCLM. This review describes the development of this Journal in light of the political situation of the time when it was founded, the situation of the publisher Walter De Gruyter after the erection of the Berlin Wall, and the development of clinical chemistry, and later on, laboratory medicine as a well-acknowledged discipline and profession. PMID:23241683

Körber, Friedrich; Plebani, Mario

2013-01-01

102

A guide to defining the competence required of a consultant in clinical chemistry and laboratory medicine  

Microsoft Academic Search

A definition has been agreed for the most senior pro- fessional (consultant) in clinical chemistry and labor- atory medicine. A model job description for a consultant has been determined, which is intended to act as a toolkit to assist employing authorities and professional bodies to define the role of individual consultant posts. A total of 86 competences for a con-

Graham Beastall; Desmond Kenny; Paivi Laitinen; Joop ten Kate

2005-01-01

103

Tandem mass spectrometry in the clinical chemistry laboratory  

Microsoft Academic Search

Tandem mass spectrometry is becoming an increasingly important analytical technology in the clinical laboratory environment. Applications in toxicology and therapeutic drug monitoring have opened the door for tandem mass spectrometry and now we are seeing a vast array of new applications being developed. It has been the combination of tandem mass spectrometry with sample introduction techniques employing atomospheric pressure ionization

Kent C. Dooley

2003-01-01

104

Clinical chemistry and molecular biology of homocysteine metabolism: An update  

Microsoft Academic Search

Objective: To summarize recent developments in our understanding of homocysteine as a clinically relevant and independent predictor of vaso-occlusive disease (including atherosclerosis and thromboembolism), as an early indicator of folate or cobalamin deficiency, and as a key factor in the pathogenesis of neural tube defects.Methods and Results: To determine total homocysteine, plasma or serum must be separated shortly after collection

Steven E. S. Miner; Jovan Evrovski; David E. C. Cole

1997-01-01

105

Self testing, an emerging component of clinical chemistry.  

PubMed

Self testing as a phase of health delivery has been utilized for approximately 70 years, but within the last two decades there has been an increasing interest in and use of self testing. The practice of self testing has a sound basis, which involves medical relevancy, cost containment, and convenience. It is reasonable to anticipate that self testing will increase in the future as medical needs and available procedures are established. The clinical chemist, the physician, and the industrial producer of test systems all have important roles in self testing. The clinical chemist, whether in a hospital laboratory or in an industrial setting, has the responsibility to create new self-testing procedures that represent expansion and improvement over those currently available. The clinical chemist also has a critical role in the evaluation of new self-testing procedures and in validating their capability of providing high-quality information. The provision of quality-assurance programs and proficiency programs for the user can most effectively be carried out by clinical chemists. The clinical chemist can also play an important role as a consultant, teacher/educator, and trouble shooter in recognizing and helping solve problems that may appear in various areas of self testing. The classic role of the physician has been to diagnose disorders of the patients and provide therapy for effective care. The role of the physician in self testing is quite comparable to his usual role, since the ultimate diagnosis of disease will be carried out by the physician and he will continue to be the source for definition of therapy. A major portion of self testing will be carried out with the recommendation of a physician, and he will maintain a role in the interpretation of results. The physician needs to be familiar with self-testing practices and procedures and be prepared to provide interpretation of self-testing results. Industry supplies an increasing proportion of reagent systems and instruments for the clinical laboratory. The supplying of reagent systems and small instruments for self testing is almost completely a role of industry. The creation and funding of new products for self testing will be provided by industry to a large extent. A critical function of industry is to provide high-quality products and efficient customer service to the self-testing component of health delivery. We have each played a role in self testing over a period of approximately 40 years. During this period self testing has become an important phase of clinical laboratory practice.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:6723038

Free, A H; Free, H M

1984-06-01

106

Frequency and relationships of clinical chemistry and liver and kidney histopathology findings in 13-week toxicity studies in rats  

Microsoft Academic Search

The relative sensitivities of eight commonly used clinical chemistry end points and histopathology to detect potential toxic effects in liver and kidney were evaluated for a series of 61 13-week rat toxicity studies conducted for the National Toxicology Program. The data consisted of 1-, 2- to 3-, and 13-week clinical chemistry measurements and 13-week histopathological assessments of liver and kidney.

G. S. Travlos; R. W. Morris; M. R. Elwell; A. Duke; S. Rosenblum; M. B. Thompson

1996-01-01

107

The XmnI polymorphic site 5' to the gene G(?) in a Brazilian patient with sickle cell anaemia - fetal haemoglobin concentration, haematology and clinical features.  

PubMed

We report a 20-year-old female with sickle cell anaemia and with an HbF concentration of 15.8%. The patient was not using hydroxyurea and was not receiving regular blood transfusions. The patient never had chronic manifestations of sickle cell anaemia, only pain crises of a mild intensity. After laboratory tests, we found that she was homozygous for HbS with the Bantu/atypical haplotype, and was heterozygous for the XmnI site. The influence of the XmnI site on the expression of HbF can explain the amelioration in clinical features in this haplotype association in a case of sickle cell anaemia. PMID:22419945

Belini Júnior, Edis; Cançado, Rodolfo D; Domingos, Claudia R B

2010-10-26

108

The XmnI polymorphic site 5' to the gene G? in a Brazilian patient with sickle cell anaemia - fetal haemoglobin concentration, haematology and clinical features  

PubMed Central

We report a 20-year-old female with sickle cell anaemia and with an HbF concentration of 15.8%. The patient was not using hydroxyurea and was not receiving regular blood transfusions. The patient never had chronic manifestations of sickle cell anaemia, only pain crises of a mild intensity. After laboratory tests, we found that she was homozygous for HbS with the Bantu/atypical haplotype, and was heterozygous for the XmnI site. The influence of the XmnI site on the expression of HbF can explain the amelioration in clinical features in this haplotype association in a case of sickle cell anaemia.

Cancado, Rodolfo D.; Domingos, Claudia R.B.

2010-01-01

109

Advances in Haematological Pharmacotherapy in 21st Century  

PubMed Central

Last quarter of twentieth century and the first 10 years of 21st century has seen phenomenal development in haematological pharmacotherapy. Tailor made chemotherapeutic agents, vast array of monoclonal antibodies, epigenetic modifiers, growth factors for red cells white cells and platelets, peptidomimetics as growth factors, newer thrombin inhibitors, safer plasma derived protein molecules, recombinant molecules, newer immunomodulators, enzyme replacement therapy and above all a plethora of targetted molecules targeting innumerable pathways involved in cell division, growth, proliferation and apoptosis has given immense number of clinically usable molecules in the hand of modern haematologists to treat diverse hitherto untreatable haematological disorders effectively. In addition many old molecules are finding newer uses in diverse fields, thalidomide as an antiangiogenic molecule is a prime example of this genre. Present overview has tried to capture this rapidly evolving area in a broad canvas without going into details of indications and contraindications of the use of various drugs.

Ghosh, Kinjalka

2010-01-01

110

Moral distress in nurses in oncology and haematology units.  

PubMed

One of the difficulties nurses experience in clinical practice in relation to ethical issues in connection with young oncology patients is moral distress. In this descriptive correlational study, the Moral Distress Scale-Paediatric Version (MDS-PV) was translated from the original language and tested on a conventional sample of nurses working in paediatric oncology and haematology wards, in six north paediatric hospitals of Italy. 13.7% of the total respondents claimed that they had changed unit or hospital due to moral distress. The items with the highest mean intensity in the sample were almost all connected with medical and nursing competence and have considerably higher values than frequency. The instrument was found to be reliable. The results confirmed the validity of the MDS-PV (Cronbach's alpha = 0.959). This study represents the first small-scale attempt to validate MDS-PV for use in paediatric oncology-haematology nurses in Italy. PMID:22457383

Lazzarin, Michela; Biondi, Andrea; Di Mauro, Stefania

2012-03-01

111

Methods for ligand-receptor assays in clinical chemistry  

SciTech Connect

This is intended to be a practical review for the clinical chemist of the laboratory procedures most commonly used to quantitate hormone receptors in various cellular fractions. These procedures include use of charcoal adsorption and hydroxylapatite for intracellular receptors and of centrifugation and filtration for membrane receptors. We discuss the use of the Scatchard analysis to establish the steroid-receptor affinity and the quantity of steroid-receptor binding sites. Both pre- and post-labeled sucrose density gradient methods are outlined. One section is devoted to the direct and indirect methods used in nuclear ''exchange'' assays. Basic theory underlying each assay is presented, but, more importantly, we assess the advantages and disadvantages of each procedure. On the basis of this information, one may decide which assay is best suited for a particular laboratory and (or) specimen.

Smith, R.G.; Sestili, M.A.

1980-04-01

112

Haematology of Persian Fallow Deer ( Dama mesopotamica )  

Microsoft Academic Search

:   The haematology of Persian fallow deer was studied and the means of various parameters were determined for sex and age groups.\\u000a For total samples, the mean ± standard deviation of haematological parameters were: red blood cells (RBC), 7.42 ± 1.27 × 1012\\/l; haematocrit (PCV), 38.83 ± 7.38%; haemoglobin (Hb), 148.0 ± 17.3 g\\/l; mean cell volume (MCV), 50.84 ±

M. Mohri; M. R. Aslani; N. Shahbazian

2001-01-01

113

Current status and future developments of LCMS\\/MS in clinical chemistry for quantification of biogenic amines  

Microsoft Academic Search

Liquid chromatography-tandem mass spectrometry (LC-MS\\/MS) is rapidly gaining ground in special clinical chemistry laboratories. It significantly increases the analytic potential in clinical chemistry, especially in the field of low molecular weight biomarker analysis. This review overviews current LC-MS\\/MS methods used for the quantification of biogenic amines and their metabolites. New possibilities offered by this technique are illustrated by recently developed

Wilhelmina H. A. de Jong; Elisabeth G. E. de Vries; Ido P. Kema

2011-01-01

114

1960 Survey of Clinical Chemistry Procedures used by Members of the American Association of Clinical Chemists  

Microsoft Academic Search

The results of the survey of the analytical procedures currently used in the clinical laboratories of members of the American Association of Clinical Chemists for the routine determination of 14 selected blood constituents are presented. The data are analyzed critically and discussed in relation to the question of standardization of bio- analyticalprocedures. THE CLINICAL LABORATORY has assumed a progressively more

Eugene W. Rice; Betty S. Grogan

115

Zygomycosis in Immunocompromised non-Haematological Patients.  

PubMed

Zygomycoses caused by fungi of the mucorales order (mucormycoses) are emerging fungal diseases with a high fatality rate. The most important risk factors include neutropenia or functional neutropenia, diabetic ketoacidosis, iron overload, major trauma, prolonged use of corticosteroids, illicit intravenous drug (ID) use, neonatal prematurity, malnourishment, and maybe a previous exposure to antifungal agents with no activity against zygomycetes, such as voriconazole and echinocandins.A high index of suspicion is crucial for the diagnosis, as prompt and appropriate management can considerably reduce morbidity and mortality. Suspicion index can be increased through recognition of the differential patterns of clinical presentation. In the non- haematological immunocompromised patients, mucormycosis can manifest in various clinical forms, depending on the underlying condition: mostly as rhino-orbital or rhino-cerebral in diabetes patients, pulmonary infection in patients with malignancy or solid organ transplantation, disseminated infection in iron overloaded or deferoxamine treated patients, cerebral - with no sinus involvement - in ID users, gastrointestinal in premature infants or malnourishment, and cutaneous after direct inoculation in immunocompetent individuals with trauma or burns.Treating a patient's underlying medical condition and reducing immunosuppression are essential to therapy. Rapid correction of metabolic abnormalities is mandatory in cases such as uncontrolled diabetes, and corticosteroids or other immunosuppressive drugs should be discontinued where feasible. AmphotericinB or its newer and less toxic lipid formulations are the drugs of choice regarding antifungal chemotherapy, while extensive surgical debridement is essential to reduce infected and necrotic tissue. A high number of cases could be prevented through measures including diabetes control programmes and proper pre- and post-surgical hygiene. PMID:21625316

Petrikkos, George; Drogari-Apiranthitou, Miranda

2011-03-15

116

Atlas of Genetics and Cytogenetics in Oncology and Haematology  

NSDL National Science Digital Library

The Atlas of Genetics and Cytogenetics in Oncology and Haematology, a peer-reviewed online journal and database provided by Infobiogen, is "devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases." Users can search the materials by genes, leukaemias, solid tumors, cancer prone diseases, and chromosomes. Researchers can find links to scientific societies and meetings. Students can explore educational materials on Mendelian and non-Mendelian Inheritance, chromosomes, population genetics, and additional human genetics topics. The website also offers reviews and case reports.

117

Effects of different centrifugation conditions on clinical chemistry and Immunology test results  

Microsoft Academic Search

Background  The effect of centrifugation time of heparinized blood samples on clinical chemistry and immunology results has rarely been\\u000a studied. WHO guideline proposed a 15 min centrifugation time without citing any scientific publications. The centrifugation\\u000a time has a considerable impact on the turn-around-time.\\u000a \\u000a \\u000a \\u000a \\u000a Methods  We investigated 74 parameters in samples from 44 patients on a Roche Cobas 6000 system, to see whether

Elisabeth I Minder; Adrian Schibli; Dagmar Mahrer; Predrag Nesic; Kathrin Plüer

2011-01-01

118

Fluorescence-lifetime-based sensing: applications to clinical chemistry and cellular imaging  

NASA Astrophysics Data System (ADS)

Measurements of fluorescence lifetimes, rather than intensity or intensity ratios, offer many advantages in clinical chemistry and imaging. However, measurements of time-resolved fluorescence are normally associated with complex laser light sources and instrumentation. In this lecture, we show how emerging technology is enabling the design and use of simple instrumentation for time-resolved fluorescence. In particular, it is now possible to imagine lifetime-based measurements of blood gases and blood glucose, and lifetime imaging of calcium and other ions in microscopic samples.

Lakowicz, Joseph R.; Szmacinski, Henryk; Thompson, Richard B.

1993-06-01

119

An approach to quality and performance control in a computer-assisted clinical chemistry laboratory.  

PubMed Central

A locally developed, computer-based clinical chemistry laboratory system has been in operation since 1970. This utilises a Digital Equipment Co Ltd PDP 12 and an interconnected PDP 8/F computer. Details are presented of the performance and quality control techniques incorporated into the system. Laboratory performance is assessed through analysis of results from fixed-level control sera as well as from cumulative sum methods. At a simple level the presentation may be considered purely indicative, while at a more sophisticated level statistical concepts have been introduced to aid the laboratory controller in decision-making processes.

Undrill, P E; Frazer, S C

1979-01-01

120

75 FR 63188 - Draft Guidance for Industry: Early Clinical Trials With Live Biotherapeutic Products: Chemistry...  

Federal Register 2010, 2011, 2012, 2013

...Trials With Live Biotherapeutic Products: Chemistry, Manufacturing, and Control Information...Trials with Live Biotherapeutic Products: Chemistry, Manufacturing, and Control Information...Trials with Live Biotherapeutic Products: Chemistry, Manufacturing, and Control...

2010-10-14

121

77 FR 9947 - Guidance for Industry: Early Clinical Trials With Live Biotherapeutic Products: Chemistry...  

Federal Register 2010, 2011, 2012, 2013

...Trials With Live Biotherapeutic Products: Chemistry, Manufacturing, and Control Information...Trials With Live Biotherapeutic Products: Chemistry, Manufacturing, and Control Information...Trials With Live Biotherapeutic Products: Chemistry, Manufacturing, and Control...

2012-02-21

122

Prevalence of rheumatic manifestations and antineutrophil cytoplasmic antibodies in haematological malignancies. A prospective study  

Microsoft Academic Search

Objective. To evaluate the prevalence of antineutrophil cytoplasmic antibodies (ANCA) and rheumatic manifestations associated with chronic haematological malignancies. Methods. Two groups of patients were prospectively studied (group I: 60 patients with myelodysplastic syndromes and group II: 140 patients with lymphoid malignancies) for clinical 'immune' manifestations and ANCA. Results. In the myelodysplastic group, six patients had ANCA-negative systemic medium- size vasculitis,

M. A. Hamidou; S. Derenne; M. A. P. Audrain; J. M. Berthelot; A. Boumalassa; J. Y. Grolleau

2000-01-01

123

Reversible phosphorylation in haematological malignancies: Potential role for protein tyrosine phosphatases in treatment?  

Microsoft Academic Search

Most aspects of leukocyte physiology are under the control of reversible tyrosine phosphorylation. It is clear that excessive phosphorylation of signal transduction elements is a pivotal element of many different pathologies including haematological malignancies and accordingly, strategies that target such phosphorylation have clinically been proven highly successful for treatment of multiple types of leukemias and lymphomas. Cellular phosphorylation status is

Roberta R. Ruela-de-Sousa; Karla C. S. Queiroz; Maikel P. Peppelenbosch; Gwenny M. Fuhler

2010-01-01

124

Haematological findings in budgerigars with megabacterium and Trichomonas infections associated with 'going light'  

Microsoft Academic Search

Eighteen budgerigars with clinical signs of 'going light' were euthanased and examined post mortem; ingluvitis caused by Trichomonas gallinae infection was present in seven birds, proventriculitis associated with the presence of megabacteria in eight birds and in three birds both conditions were present. Haematological examinations of blood taken from the living birds showed that those with T gallinae infection had

GM Henderson; FM Gulland; CM Hawkey

1988-01-01

125

Clinical chemistry and laboratory medicine in Croatia: regulation of the profession.  

PubMed

Heterogeneity exists across Europe in the definition of the profession of clinical chemistry and laboratory medicine and also in academic background of specialists in this discipline. This article provides an overview of the standards of education and training of laboratory professionals and quality regulations in Croatia. Clinical chemistry in Croatia is almost exclusively practiced by medical biochemists. Although term Medical biochemist often relates to medical doctors in other European countries, in Croatia medical biochemists are not medical doctors, but university degree professionals who are qualified scientifically. Practicing the medical biochemistry is regulated by The Health Care Law, The Law of the Medical Biochemistry Profession and The Law of the State and Private Health Insurance. According to the law, only medical biochemists are entitled to run and work in the medical biochemistry laboratory. University degree is earned after the 5 years of the studies. Register for medical biochemists is kept by the Croatian Chamber of Medical Biochemists. Licensing is mandatory, valid for 6 years and regulated by the government (Law on the Health Care, 1993). Vocational training for medical biochemists lasts 44 months and is regulated by the national regulatory document issued by the Ministry of Health. Accreditation is not mandatory and is provided by an independent, non-commercial national accreditation body. The profession has interdisciplinary character and a level of required competence and skills comparable to other European countries. PMID:22141201

Simundic, Ana-Maria; Topic, Elizabeta; Cvoriscec, Dubravka; Cepelak, Ivana

2011-01-01

126

A history of HbA1c through Clinical Chemistry and Laboratory Medicine.  

PubMed

HbA(1c) was discovered in the late 1960s and its use as marker of glycemic control has gradually increased over the course of the last four decades. Recognized as the gold standard of diabetic survey, this parameter was successfully implemented in clinical practice in the 1970s and 1980s and internationally standardized in the 1990s and 2000s. The use of standardized and well-controlled methods, with well-defined performance criteria, has recently opened new directions for HbA(1c) use in patient care, e.g., for diabetes diagnosis. Many reports devoted to HbA1c have been published in Clinical Chemistry and Laboratory Medicine (CCLM) journal. This review reminds the major steps of HbA(1c) history, with a special emphasis on the contribution of CCLM in this field. PMID:22992284

Gillery, Philippe

2013-01-01

127

Haematological parameters of the otrich (Struthio camelus)  

Microsoft Academic Search

Haematological reference values according to age and sex, were obtained from healthy ostriches (Struthio camelus) held under intensive conditions. Erythrocytic parameters and indices demonstrated an age?related trend: the packed cell volume, haemoglobin concentration and erythrocyte count were lowest in ostriches of 1 to 3 months of age. The mean corpuscular volume increased with age until adulthood. No sex?linked differences were

A. Levi; B. Perelman; T. Waner; M. Van Grevenbroek; C. Van Creveld; R. Yagil

1989-01-01

128

Intestinal helminths induce haematological changes in dogs from Jabalpur, India.  

PubMed

The effect of canine intestinal helminths on the haematological profile of 200 dogs, of both sexes and variable age, visiting university veterinary clinics for routine examination was investigated. The dogs were assigned to parasitized (n = 39) and non-parasitized (n = 161) groups of animals. Coprological examination revealed a 19.5% prevalence of different species of the helminths. Of these animals, 10.25% had mixed infections with Ancylostoma caninum, Toxascaris spp. and Dipylidium caninum. The intensity of A. caninum infection was the highest, with mean egg counts of 951.43 (standard error 88.66), followed by Toxascaris 283.33 (standard error 116.81) and D. caninum. The parasitized animals had significantly lower levels of haemoglobin, packed cell volume and total erythrocyte counts than non-parasitized animals (P < 0.01). Values of other parameters, except for lymphocytes and eosinophils, were not different between the two groups. Analyses of the haematological profile revealed normocytic hypochromic anaemia in the parasitized group of animals. PMID:21110909

Qadir, S; Dixit, A K; Dixit, P; Sharma, R L

2010-11-29

129

Where are we at with point-of-care testing in haematology?  

PubMed

Point-of-care testing (POCT) in haematology has continued to grow in popularity and uptake throughout the world. The increasing demand to reduce the turnaround time of test results, coupled with rapid improvements in technology, have led to the development of several devices that are designed for use in different clinical settings, with the hope of improving patient care. The most used POCT in haematology is measurement of haemoglobin concentration. Other POCT devices (used primarily in developing countries) for malaria screening and CD4+ T-lymphocytes for quantification of human-immunodeficiency-virus are becoming the cornerstone for the diagnosis and management of these disorders. New devices are also available for red cell indices, white blood cell count and platelets. In this review clinical studies that validate the use of such devices will be discussed, as well as the advantages and disadvantages of POCT in haematology. A disadvantage of POCT is a lack of training, poor standardization in obtaining blood samples and insufficient internal/external quality assessment. As there is every reason to expect that POCT use will increase in all pathology disciplines, including haematology, it is imperative that systems are put in place to oversee these issues. PMID:22765160

Briggs, Carol; Kimber, Simon; Green, Laura

2012-07-05

130

Huperzine a as potential treatment of Alzheimer's disease: an assessment on chemistry, pharmacology, and clinical studies.  

PubMed

Alzheimer's disease (AD) is the fourth leading cause of death in adults, characterized by hallmark neuritic plaques and neurofibrillary tangles. Current treatments focus only on symptom relief. As a possible new treatment option for AD, huperzine A's chemistry, pharmacology, and clinical effectiveness are assessed. The chemical synthesis of huperzine A has been optimized, while an in vitro technique has provided a renewable plant source. Pharmacological studies showed that the drug inhibits the enzyme acetylcholinesterase reversibly and selectively. Huperzine A also displayed good pharmacokinetics with a rapid absorption and a wide distribution in the body at a low to moderate rate of elimination. Presently, inadequate toxicity data in human have been reported, yet animal studies demonstrated mild to moderate cholinergic side effects at therapeutic doses. Previous clinical trials have shown improvement in memory function using MMSE, MQ, ADAS-COG, and ADL tests. In an unpublished phase II clinical trial, the ADAS-COG and MMSE tests indicated cognitive enhancement at a dose of 0.4?mg, yet no improvement was observed at a dose of 0.2?mg. The MMSE scores indicated cognitive enhancement at 0.4?mg. Promising data suggested that huperzine A is well tolerated at doses up to 0.4?mg for 24 weeks. Therefore, huperzine A seems to be a potential treatment option for AD. PMID:21766442

Ha, Giang T; Wong, Ryan K; Zhang, Yan

2011-07-01

131

Update on cardiovascular safety of PPARgamma agonists and relevance to medicinal chemistry and clinical pharmacology.  

PubMed

Peroxisome proliferator-activator receptors (PPARs) are now known as members of the nuclear hormonereceptor superfamily of ligand-activated transcription factors that regulate gene expression in response to nutritional and physiological stimuli. PPAR? plays a crucial role in glucose homeostasis and it is involved in the regulation of lipid metabolism and adipocyte differentiation and function. From all the PAAR? ligands, the thiazolidindiones (TZDs) are of most clinical importance. Rosiglitazone and pioglitazone have been largely used so far in the clinical practice. They provide similar effects on glycemic control, as well as a range of similar adverse effects, such as weight gain, fluid retention, and increased risk of hearth failure, which seem to be PPAR? mediated. Interestingly, they differ on their effect on lipid and cardiovascular safety profile, indicating a PPAR?-independent mechanism. Indeed, rosiglitazone was recently withdrawn in Europe and its use has been restricted in USA as a consequence of increased risk of cardiovascular events in type 2 diabetic patients. This review is focused on the cardiovascular effects of rosiglitazone and pioglitazone as representative members of PPAR? ligands, because they were widely evaluated in many clinical trials and experimental studies and data obtained from these studies are relevant from medicinal chemistry and clinical pharmacology point of view. Finally, an overview on the development of selective PPAR? modulators and/or dual PPAR?/? agonists will be given. These new approaches might provide anti-hyperglycemic efficacy without the associated undesirable side-effects. However, further experimental and clinical studies evaluating the theoretical benefit and safety of this therapeutic strategy are needed. PMID:22242856

Ciudin, Andreea; Hernandez, Cristina; Simó, Rafael

2012-01-01

132

Haematological Alterations Observed in Equine Routine Complete Blood Counts. A Retrospective Investigation  

Microsoft Academic Search

:   Results on more than 1000 complete blood counts (CBC) performed on hot-blooded horses, including Thoroughbred, Standardbred,\\u000a and Arabian, have been reviewed. All the data were processed and stored at the Laboratory of Haematology, Department Veterinary\\u000a Clinic, University of Pisa, over the last 2 years, and involved samples withdrawn from both inpatients and outpatients of\\u000a the clinic.\\u000a \\u000a ?The CBC is

A. GAVAZZA; A. J. DELGADILLO; B. GUGLIUCCI; A. PASQUINI; G. LUBAS

2002-01-01

133

Haematological malignancies: the rationale for integrated haematopathology services, key elements of organization and wider contribution to patient care.  

PubMed

The National Institute for Clinical Excellence (NICE) defined the process of care for haematological malignancies in Improving outcomes in haematological cancers. The manual in 2003. The most challenging recommendation has been the requirement to develop integrated laboratory services for accurate diagnosis. This is an aim that has not been fully achieved. The unified concept of haematological malignancy is recent and based on an understanding of the cellular pathology of the bone marrow and immune systems. Historical UK pathology practice has previously resulted in the separation of laboratory haematology from histopathology and of liquid and tissue specimens. Proposals for reintegration and centralization with specialist-led, centralized diagnostic and reporting services challenge the fragmented historical model. Accuracy and certainty of diagnosis remains problematic, particularly for lymphomas, with evidence that the accuracy of diagnosis is slowly improving but still only approaches 85%. There is a potentially significant human and financial cost of diagnostic errors. No nationwide, validated and comparable epidemiology/population-based data exist for accurate and complete ascertainment of new cases of haematological cancer, service planning or clinical outcomes monitoring. This article examines the original rationale behind the NICE guidance and outlines the key components and processes of an integrated diagnostic service. PMID:21261689

Ireland, Robin

2011-01-01

134

Characterization of Rheumatoid Arthritis Subtypes Using Symptom Profiles, Clinical Chemistry and Metabolomics Measurements  

PubMed Central

Objective The aim is to characterize subgroups or phenotypes of rheumatoid arthritis (RA) patients using a systems biology approach. The discovery of subtypes of rheumatoid arthritis patients is an essential research area for the improvement of response to therapy and the development of personalized medicine strategies. Methods In this study, 39 RA patients are phenotyped using clinical chemistry measurements, urine and plasma metabolomics analysis and symptom profiles. In addition, a Chinese medicine expert classified each RA patient as a Cold or Heat type according to Chinese medicine theory. Multivariate data analysis techniques are employed to detect and validate biochemical and symptom relationships with the classification. Results The questionnaire items ‘Red joints’, ‘Swollen joints’, ‘Warm joints’ suggest differences in the level of inflammation between the groups although c-reactive protein (CRP) and rheumatoid factor (RHF) levels were equal. Multivariate analysis of the urine metabolomics data revealed that the levels of 11 acylcarnitines were lower in the Cold RA than in the Heat RA patients, suggesting differences in muscle breakdown. Additionally, higher dehydroepiandrosterone sulfate (DHEAS) levels in Heat patients compared to Cold patients were found suggesting that the Cold RA group has a more suppressed hypothalamic-pituitary-adrenal (HPA) axis function. Conclusion Significant and relevant biochemical differences are found between Cold and Heat RA patients. Differences in immune function, HPA axis involvement and muscle breakdown point towards opportunities to tailor disease management strategies to each of the subgroups RA patient.

van der Kooij, Anita J.; Reijmers, Theo H.; Schroen, Yan; Wang, Mei; Xu, Zhiliang; Wang, Xinchang; Kong, Hongwei; Xu, Guowang; Hankemeier, Thomas; Meulman, Jacqueline J.; van der Greef, Jan

2012-01-01

135

Combined Shewhart-cusum control chart for improved quality control in clinical chemistry.  

PubMed

We describe the adaptation of the decision limit cumulative sum method (cusum) to internal quality control in clinical chemistry. With the decision limit method, the cusum is interpreted against a numerical limit, rather than by use of a V-mask. The method can be readily implemented in computerized quality-control systems or manually on controls charts. We emphasize the manual application here and demonstrate how the technique can be implemented on existing Shewhart or Levey-Jennings control charts. This permits both cusum and Shewhart control rules to be used simultaneously on a single control chart and also minimizes the data calculations necessary for the cusum method. Computer simulation studies are used to determine the performance characteristics of several different cusum rules, alone and in combination with a Shewhart rule. These studies indicate that improvements in existing quality-control systems should be possible by addition of this simple cusum method and by use of a combined Shewhart-cusum control chart. This should be particularly advantageous when introducing the cusum method in laboratories with manual quality-control systems. PMID:902415

Westgard, J O; Groth, T; Aronsson, T; de Verdier, C H

1977-10-01

136

Haematological and morphological responses of broiler chicks to hypoxia  

Microsoft Academic Search

Broiler chicks were subjected to experimentally?induced hypoxia and the haematology, together with the histopathology and ultrastructure of heart, liver, lung and kidney and the ultracytochemistry of heart tissues were examined. The haemoglobin concentration, packed cell volume and red blood cell counts were significantly increased compared with controls. The results resembled the haematology of similar aged broilers with an ascitic syndrome

M. H. Maxwell; S. Spence; G. W. Robertson; M. A. Mitchell

1990-01-01

137

Use of Antibiotics in a Haematology Ward - An Audit  

Microsoft Academic Search

Introduction: Rising rates of antibiotic resistance prompted a review of antibiotic use policies hospitalwide. The Department of Haematology established a new set of consensus guidelines in 2002 for antibiotic use in febrile neutropenia. The aim of our study was to audit adherence to the guidelines established for febrile neutropenia in patients treated for haematologic malignancies. Materials and Methods: An antibiotic

Gee Chuan Wong; Ban Hock Tan; Wong Gee Chuan

2008-01-01

138

Clinical chemistry of congenic mice with quantitative trait loci for predicted responses to Trypanosoma congolense infection.  

PubMed

Trypanosoma congolense is a protozoan parasite that causes severe diseases in livestock. Three major quantative trait loci (QTL), Tir1, Tir2, and Tir3, control the survival time of mice after infection with T. congolense. Congenic mice carrying the C57BL/6 resistance alleles on the A/J background were developed for each of these loci. The congenic mice were used to physically map the regions containing the QTL gene(s) and to investigate the physiological effect of each locus. Clinical chemistry data for infected A/J, C57BL/6, and BALB/c mice were obtained for 15 analytes at five time points. Congenic mice were assessed for survival, parasitemia, and anemia as well as seven clinical-chemical analytes. The survival times were significantly increased in the Tir1 and Tir2 mice but not Tir3 congenic mice. The survival time of the parental inbred mice correlated negatively with parasitemia but positively with alanine aminotransferase activities in serum, suggesting that inflammatory reactions in the liver had a beneficial effect possibly associated with reduced parasitemia. However, there was no difference in parasitemia or liver enzyme activities of Tir1 and Tir2 congenic mice relative to their controls, showing that survival, parasitemia, and degree of liver damage are not associated with each other, despite the correlation in the parental lines. These data suggest that the congenic loci affect survival but do not affect control of parasite number. They may therefore act by limiting the pathological consequences of T. congolense infection. PMID:19596769

Rathkolb, Birgit; Noyes, Harry A; Brass, Andy; Dark, Paul; Fuchs, Helmut; Gailus-Durner, Valérie; Gibson, John; de Angelis, Martin Hrabé; Ogugo, Moses; Iraqi, Fuad; Kemp, Steve J; Naessens, Jan; Pope, Mathew E; Wolf, Eckhard; Agaba, Morris

2009-07-13

139

Alterations in Coagulation Profiles and Biochemical and Haematological Parameters in Cattle with Traumatic Reticuloperitonitis  

Microsoft Academic Search

This study was designed to investigate alterations in coagulation, and in biochemical and haematological parameters in cattle\\u000a with traumatic reticuloperitonitis (TRP). In the study, 28 dairy cattle with TRP and 10 clinically healthy cattle (control)\\u000a of different ages and breeds were used. Cattle with TRP had prolonged prothrombin time (PT), thrombin time (TT) and activated\\u000a partial thromboplastin time (APTT). Erythrocytopenia,

H. I. Gokce; G. Gokce; M. Cihan

2007-01-01

140

Haematological and haematochemical responses to training and competition in standardbred horses  

Microsoft Academic Search

The aim of this study was to evaluate haematological and haematochemical responses to training and competition in standardbred\\u000a horses. We investigated the modifications that occur in athletic horses during different workloads of training and after official\\u000a competitions of 1,600 and 2,000 m. The study was carried out on 15 gelding clinically healthy horses. The animals were divided\\u000a into three groups: group

Giuseppe Piccione; Stefania Casella; Claudia Giannetto; Vanessa Messina; Vincenzo Monteverde; Giovanni Caola; Sergio Guttadauro

2010-01-01

141

Acid–base chemistry of plasma: consolidation of the traditional and modern approaches from a mathematical and clinical perspective  

Microsoft Academic Search

Objective  Debate still exists as to whether the Stewart (modern) or traditional model of acid–base chemistry is best in assessing the\\u000a acid–base status of critically ill patients. Recent studies have compared various parameters from the modern and traditional\\u000a approaches, assessing the clinical usefulness of parameters such as base excess, anion gap, corrected anion gap, strong ion\\u000a difference and strong ion gap.

S. Matousek; J. Handy; S. E. Rees

2011-01-01

142

Histopathology and serum clinical chemistry evaluation of broilers with femoral head separation disorder  

Technology Transfer Automated Retrieval System (TEKTRAN)

Femoral head disarticulation (FHD) and necrosis is a sporadic leg problem of unknown etiology in broiler breeders. To determine the underlying physiology of FHD, the blood chemistry and the histopathology of the femoral heads of the affected chickens were compared with their age matched controls. Ch...

143

A contribution for the definition of serum chemistry values in captive adults Antillean manatees (Trichechus manatus manatus Linnaeus, 1758).  

PubMed

Serum chemistry analyses represents a fundamental tool for the diagnosis and understanding of diseases in marine mammals. Although several studies are being conducted within the field of clinical pathology, haematological and serum chemistry data for Antillean manatees are deficient. The purpose of this study was to determine serum chemistry values for captive Antillean manatees within the CMA/Ibama facility in Brazil. Serum samples were obtained from five captive adult Antillean manatees fed with seagrass and analysed for aspartate aminotransferase, alanine aminotransferase, bilirubin, alkaline phosphatase, urea, creatinine, glucose, triglycerides, cholesterol, total protein, albumin, globulin, phosphate, chloride, calcium and uric acid. Blood chemistry parameters were determined using a semi-automatic analyzer. Maximum, minimum, mean and standard deviations were calculated for each serum chemistry parameter. Differences on the values of males and females were verified using an unpaired Student's t-test. All the parameters analysed were similar between sexes, with exception of AP, which was higher in females (191.43 +/- 31.86 U/l). Alanine aminotransferase and uric acid values for Trichechus manatus manatus are reported for the first time in this paper. This study is the first to report serum chemistry parameter values for long-term captive male and female Antillean manatees. Therefore, the lower values of albumin, phosphate, chloride, cholesterol and triglycerides obtained here highlight the importance of clinical pathology during health monitoring of captive marine mammals. PMID:17381673

Silva, F M O; Vergara-Parente, J E; Gomes, J K N; Teixeira, M N; Lima, R P

2007-04-01

144

The relationship between cardiac and liver iron evaluated by MR imaging in haematological malignancies and chronic liver disease  

PubMed Central

Although iron overload is clinically significant, only limited data have been published on iron overload in haematological diseases. We investigated cardiac and liver iron accumulation by magnetic resonance imaging (MRI) in a cohort of 87 subjects who did not receive chelation, including 59 haematological patients. M-HIC (MRI-based hepatic iron concentration, normal values <36??mol/g) is a non-invasive, liver biopsy-calibrated method to analyse iron concentration. This method, calibrated to R2 (transverse relaxation rate), was used as a reference standard (M-HIC(R2)). Transfusions and ferritin were evaluated. Mean M-HIC(R2) and cardiac R* of all patients were 142??mol/g (95% CI, 114–170) and 36.4?1/s (95% CI, 34.2–38.5), respectively. M-HIC(R2) was higher in haematological patients than in patients with chronic liver disease or normal controls (P<0.001). Clearly elevated cardiac R2* was found in two myelodysplastic syndrome (MDS) patients with severe liver iron overload. A poor correlation was found between liver and cardiac iron (n=82, r=0.322, P=0.003), in contrast to a stronger correlation in MDS (n=7, r=0.905, P=0.005). In addition to transfusions, MDS seemed to be an independent factor in iron accumulation. In conclusion, the risk for cardiac iron overload in haematological diseases other than MDS is very low, despite the frequently found liver iron overload.

Virtanen, J M; Remes, K J; Itala-Remes, M A; Saunavaara, J P; Komu, M E; Partanen, A M; Parkkola, R K

2012-01-01

145

Malaria and haematologic parameters of pupils at different altitudes along the slope of Mount Cameroon: a cross-sectional study  

PubMed Central

Background Haematologic abnormalities are features in Plasmodium falciparum infection, and anaemia is an inevitable outcome. This study examines the influence of malaria status and altitude on haematologic parameters in school-aged pupils. Methods A cross-sectional study was conducted among 728 school pupils aged between four and 15 years at three different altitudinal ranges along the slope of the Mount Cameroon region. The investigative methods included the use of questionnaire, clinical evaluation and laboratory investigations. Blood sample collected from each child was used for the preparation of blood films for detection of malaria parasites and assessment of malaria parasite density as well as full blood count determination using an automated haematology analyzer. Results The prevalence of malaria in the study population was 33.8% and 64.2% (158/246) of these were asymptomatic (AM). Pupils in lowlands had a significantly (P <0.05) prevalence (95% confidence interval, CI) of malaria (60.6%, CI?=?54.6–65.9%) than those in middle belt (29.1%, CI?=?23.9–34.8%) and highlands (7.7%, CI?=?6.1–9.8%), while those in middle belt had significantly higher geometric mean parasite density (475) than those in lowlands (233) and highlands (388). The prevalence of malaria was significantly higher in children that presented with fever (40.4%, CI?=?33.8–47.2%) when compared with afebrile subjects (31%, CI?=?27–35.2%). Pupils with AM had a higher prevalence of leucopaenia (43.7%, CI?=?35.8–51.8%), microcytosis (27.2%, CI?=?20.5–34.9%), hypochromasia (27.8%, CI?=?21–35.5%) and thrombocytopaenia (14.9%, CI?=?8.9–22.8%) when compared with those with clinical malaria (CM). All mean haematological parameters were comparable in pupils with CM and AM, except for the mean white blood cell (WBC) counts. Pupils with AM had significantly lower (P?=?0.02) mean WBC counts (5.1?±?2.5?×?109/L) than those with CM (5.9?±?2.3?×?109/L). Age, altitude and malaria parasitaemia was of significant influence on several haematological parameters. Conclusion Altitude influenced the distribution and density of malaria parasites and was of confounding influence on the haematologic profiles. These results highlight the insidious effects of AM on the haematologic components.

2013-01-01

146

Changed clinical chemistry pattern in blood after removal of dental amalgam and other metal alloys supported by antioxidant therapy.  

PubMed

This study aimed to investigate a possible connection between removal of dental amalgam restorations supported by antioxidant therapy and indicative changes of clinical chemistry parameters. A group of 24 patients, referred for complaints related to amalgam restorations, underwent a removal of their amalgams. All patients were treated with antioxidants (vitamin B-complex, vitamin C, vitamin E, and sodium selenite). An age- and sex-matched control group of 22 individuals was also included. The mercury (Hg) and selenium (Se) concentration in plasma, Hg concentration in erythrocytes, and 17 clinical chemistry variables were examined in three groups: patients before amalgam removal (Before), patients after amalgam removal (After), and control individuals (Control). The Hg and Se values decreased (p < 0.05) in plasma, and the Hg concentration decreased (p < 0.05) in erythrocytes after amalgam removal. The variables serum lactate dehydrogenase (serum LDH) and serum sodium differed significantly both when comparing Control with Before (p < 0.01) and Before with After (p < 0.01). The variables white blood cell count (WBC), blood neutrophil count, blood eosinophil count, blood basophil count, blood lymphocyte count, blood monocyte count, serum potassium, and serum creatinine differed in the Before/After test (p < 0.05). Multivariate statistics (discriminant function analysis) could separate the groups Before and After with only one misclassification. PMID:17916968

Frisk, Peter; Danersund, Antero; Hudecek, Romuald; Lindh, Ulf

2007-01-01

147

Fifteenth biannual report of the Cochrane Haematological Malignancies Group--focus on non-Hodgkin's lymphoma.  

PubMed

This fifteenth biannual report of the Cochrane Haematological Malignancies Group (CHMG) highlights recently published randomized controlled trials (RCTs) in the field of hemato-oncology, covering the publication period from October 2011 to May 2012. Implications for clinical practice and methodological aspects are the main principles for selecting trials for this report. Studies were identified by electronic search of MEDLINE using a broad search filter that covers all topics in hemato-oncology combined with a highly sensitive search filter for randomized trials (Cochrane Handbook for Systematic Reviews of Interventions). PMID:23852951

Rancea, Michaela; Will, Andrea; Borchmann, Peter; Monsef, Ina; Engert, Andreas; Skoetz, Nicole

2013-07-12

148

Twelfth biannual report of the Cochrane Haematological Malignancies Group--focus on tyrosine kinase inhibitors.  

PubMed

This 12th biannual report of the Cochrane Haematological Malignancies Group highlights recently published randomized controlled trials in the field of hemato-oncology, covering the publication period from September 1, 2009, through June 30, 2010. Implication for clinical practice and methodological aspects are the main principles used to select trials for this report. Studies on tyrosine kinase inhibitors for patients with chronic myeloid leukemia were identified through electronic search of MEDLINE with a broad search filter that covered all topics in hemato-oncology combined with a highly sensitive search filter for randomized studies as described in the Cochrane Handbook for Systematic Reviews of Interventions. PMID:21252264

Skoetz, Nicole; Kluge, Sabine; Monsef, Ina; Bauer, Kathrin; Siebert, Hendrik; Kreuzer, Karl-Anton; Engert, Andreas

2011-01-20

149

International Osteoporosis Foundation and International Federation of Clinical Chemistry and Laboratory Medicine position on bone marker standards in osteoporosis.  

PubMed

The International Osteoporosis Foundation (IOF) and the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Working Group on Bone Marker Standards (WG-BMS) has evaluated the clinical potential of bone turnover markers (BTMs) in the prediction of fracture risk and for monitoring treatment. Research evidence suggests that BTMs may provide information on fracture risk independently from BMD, so that fracture risk prediction might be enhanced by their inclusion in assessment algorithms. The potential use of BTMs to predict the response to treatments for osteoporosis in the individual patient is also of great interest. Treatment-induced changes in specific markers account for a substantial proportion of fracture risk reduction. However, there is still a need for stronger evidence on which to base practice in both situations. IOF/IFCC recommends one bone formation marker (serum procollagen type I N propeptide, s-PINP) and one bone resorption marker (serum C-terminal cross-linking telopeptide of type I collagen, s-CTX) to be used as reference markers and measured by standardised assays in observational and intervention studies in order to enlarge the international experience of the application of markers to clinical medicine and to help resolve uncertainties over their clinical use. PMID:21605012

Vasikaran, Samuel; Cooper, Cyrus; Eastell, Richard; Griesmacher, Andrea; Morris, Howard A; Trenti, Tommaso; Kanis, John A

2011-05-24

150

Clinical pharmacology in normal volunteers of praziquantel, a new drug against schistosomes and cestodes  

Microsoft Academic Search

The tolerance of Praziquantel (2-cyclohexylcarbonyl-1, 3, 4, 6, 7, 11b-hexahydro-2H-pyrazino-[2, 1-a]isoquinoline-4-one) in oral doses of 1×20 mg\\/kg, 1×50 mg\\/kg, 3×10 mg\\/kg and 3×25 mg\\/kg body weight (t=4 h) was tested in a complex study involving 36 healthy volunteers. In addition to the usual assessment of clinical chemistry, haematology, coagulation physiology, urinalysis, clinico-physiological examination including EEG, and medical examination, clinico-psychological parameters

G. Leopold; W. Ungethiim; E. Groll; H. W. Diekmann; H. Nowak; D. H. G. Wegner

1978-01-01

151

Monitoring trough voriconazole plasma concentrations in haematological patients: real life multicentre experience.  

PubMed

The objective of this retrospective study was to evaluate results from voriconazole therapeutic drug monitoring (TDM) in haematological patients in routine clinical practice. Between 2005 and 2010, 1228 blood samples were obtained from 264 haematological patients (median 3 samples/patient; range 1-27) receiving voriconazole for targeted/preemptive treatment of invasive aspergillosis (IA) (46.3% of samples), empirical therapy (12.9%) or prophylaxis (40.8%). A high-pressure liquid chromatography assay was used to analyse voriconazole concentrations. Clinical and laboratory data were analysed retrospectively. The median of the detected voriconazole plasma concentration was 1.00 ?g ml(-1) (range <0.20-13.47 ?g ml(-1)). Significant inter- and intra-patients variability of measured concentrations (81.9% and 50.5%) were identified. With the exception of omeprazole administration, there was no relevant relationship between measured voriconazole concentrations and drug dose, route administration, age, gender, CYP2C19*2 genotype, gastrointestinal tract abnormality, administration via nasogastric tube, serum creatinine, and liver enzymes. However, per patient analysis identified significant role of individual voriconazole dose and drug form change on measured plasma concentration. Measured voriconazole concentrations did not correlate with the treatment outcome of patients with IA. We only identified a limited number of adverse events related to voriconazole therapy; however, the median plasma concentration was not different from concentrations measured in samples without reported toxicity. Our retrospective study has suggested that routine monitoring of voriconazole plasma concentrations has probably only a limited role in daily haematological practice. PMID:22429709

Racil, Zdenek; Winterova, Jana; Kouba, Michal; Zak, Pavel; Malaskova, Ludmila; Buresova, Lucie; Toskova, Martina; Lengerova, Martina; Kocmanova, Iva; Weinbergerova, Barbora; Timilsina, Shira; Rolencova, Monika; Cetkovsky, Petr; Mayer, Jiri

2012-03-19

152

Histopathology and serum clinical chemistry evaluation of broilers with femoral head separation disorder.  

PubMed

Femoral head separation (FHS) and necrosis is a sporadic leg problem of unknown etiology in broiler breeders. To determine the underlying physiology of FHS, the blood chemistry and histopathology of the femoral growth plates of the affected chickens were compared with their age-matched controls and with birds having tibial dyschondroplasia. Femoral problems were categorized on the basis of 1) femoral head separating from articular cartilage without any visible damage to the growth plate (FHS) and 2) FHS with significant tearing and lesions in the growth plate (FHSL). Tibial dyschondroplasia was identified by a widening of the growth plate with an unresorbed plug of cartilage at the proximal end of the tibia. Control birds were without any femoral or tibial problems. The histopathology of FHSL growth plates revealed occasional chondrocyte death, hypocellularity, dysplasia in the prehypertrophic zones, and the absence of inflammatory infiltrates in the lesion areas. Hematoxylin and eosin staining showed brown chromogenic deposits in the metaphyseal bone marrow areas. Blood chemistry of chickens with FHSL showed a modest but significant elevation of cholesterol, triglycerides, and low-density lipoproteins. Only cholesterol and low-density lipoproteins were moderately elevated in FHS-affected chickens. Other blood parameters, such as protein, magnesium, and iron levels, showed differential changes in birds with leg problems, but there were no specific trends. Neither blood ovotransferrin, a marker of chronic inflammation, nor corticosterone, a marker of stress, showed any significant differences from the controls. These results indicate that FHS may be a metabolic problem in poultry, one that is related to fat metabolism disorders, possibly contributing to an unbalanced growth in the articular-epiphyseal complex that leads to its separation under sheer stress. PMID:19431999

Durairaj, V; Okimoto, R; Rasaputra, K; Clark, F D; Rath, N C

2009-03-01

153

A comparative survey of the results of analyses of blood serum in clinical chemistry laboratories in the United Kingdom  

PubMed Central

Since July 1969, portions of the same blood serum have been dispatched to clinical chemistry laboratories in the United Kingdom at 14-day intervals. The results of each serum survey were reported to each of the 390 participants within 11 days of their originally receiving the specimen. During the first 18 months of the survey no overall improvement in the results was seen. Therefore a summary of each laboratory's ability consistently to produce results close to the mean of the method used was calculated and reported as a single figure, the variance index, and sent to all participants at regular intervals together with a histogram distribution of the variance indices of other participants. The subsequent improvement in the overall results is described. Images

Whitehead, T. P.; Browning, D. M.; Gregory, A.

1973-01-01

154

New category of probable invasive pulmonary aspergillosis in haematological patients.  

PubMed

The European Organization for Research and Treatment of Cancer and the Mycosis Study Group (EORTC-MSG) radiological definitions of invasive pulmonary aspergillosis (IPA) may lack diagnostic sensitivity. We evaluated applying less restrictive radiological criteria, when supported by specific microbiological findings, to define IPA in acute myeloid leukaemia (AML), lymphoproliferative diseases (LD) and allogeneic stem cell transplant (allo-SCT) patients. Overall, 109 consecutive episodes of proven/probable IPA in 56 AML, 31 LD and 22 allo-SCT patients diagnosed from February 2006 through to January 2011 were considered. IPA was diagnosed with EORTC-MSG criteria (control group, 76 patients) or without prespecified radiological criteria (study group, 33 patients). The latter differed from the former by the inclusion of patients with pulmonary infiltrates not fulfilling the three EORTC-MSG IPA specific findings of dense, well-circumscribed lesions with or without halo sign, air crescent sign or cavity. All the analysed clinical and mycological characteristics, 3-month response to antifungal therapy and 1- and 3-month cumulative survival were comparable in the control and study groups in AML, LD and allo-SCT patients. Seventeen of 33 (51.5%) patients of the study group fulfilled EORTC-MSG radiological criteria at subsequent imaging performed a median of 15 days (range, 6-40 days) after documentation of the pulmonary infection. Our study seems to confirm the possibility of revising the EORTC-MSG criteria by extending the radiological suspicion of IPA to less specific chest computerized tomography scan findings when supported by microbiological evidence of Aspergillus infection in high-risk haematological patients. PMID:21999366

Girmenia, C; Guerrisi, P; Frustaci, A M; Fama, A; Finolezzi, E; Perrone, S; Gentile, G; Collerone, F; Brocchieri, S; Guerrisi, V

2011-10-17

155

Haematological and biochemical parameters during pregnancy and lactation in sows  

Microsoft Academic Search

The aim of this research was to describe changes in serum concentrations of some micronutrients, and haematological parameters during pregnancy and lactation of sows. Data presented here were obtained by using blood samples from healthy, conventionally managed sows from a breeding herd. The samples were taken at three different points in the physiological production process: I. from the 30th to

Vladimir Mrljak; Jelena Pompe Gotal

156

Variation in haematological parameters among inbred strains of rat  

Microsoft Academic Search

Summary Haematological determinations were carried out on 7 inbred strains of rats using a standardized procedure. Significant genetic variation was found for many of the parameters although none of the strains had values which could be termed pathological. Measurements also varied significantly from day to day. Factorial analysis of variance is shown to be a powerful method for the investigation

D. P. Lovell; R. K. Archer; Joan Riley; R. K. Morgan

1981-01-01

157

Haematological studies of Emblica officinalis formulation on Wistar Rats  

Microsoft Academic Search

Over three-quarters of the world population relies mainly on plants and plant extracts for health care. More than 30% of the entire plant species were used for medicinal purposes. Emblica officinalis is one of the widely used food supplement for the prevention of many ailments. A comprehensive account of the effect of Emblica officinalis formulation on some of the haematological

Narendra Kumar Savala; Parvathaneni Nagasri Haritha; Mangamoori Lakshmi Narasu

2012-01-01

158

Is intensive care justified for patients with haematological malignancies?  

Microsoft Academic Search

The course of 260 adults with haematological malignancies admitted to a medical intensive care unit was studied to evaluate the value of life support techniques and to research predictive factors. The overall in the medical intensive care unit (MICU) and hospital mortality rates were respectively 43% (113 patients) and 57% (148 patients). Among survivors, 64% (49 patients) were still alive

F. Brunet; J. J. Lanore; J. F. Dhainaut; F. Dreyfus; J. F. Vaxelaire; S. Nouira; T. Giraud; A. Armaganidis; J. F. Monsallier

1990-01-01

159

A Competency-Based Clinical Chemistry Course for the Associate Degree Medical Laboratory Technician Graduate in a Medical Technology Baccalaureate Program.  

ERIC Educational Resources Information Center

Presented is a project that developed a competency-based clinical chemistry course for associate degree medical laboratory technicians (MLT) in a medical technology (MT) baccalaureate program. Content of the course was based upon competencies expected of medical technologists at career-entry as defined in the statements adopted in 1976 by the…

Buccelli, Pamela

160

RefVal: a program implementing the recommendations of the International Federation of Clinical Chemistry on the statistical treatment of reference values  

Microsoft Academic Search

RefVal is a computer program that implements the recommendations of the International Federation of Clinical Chemistry on the statistical treatment of reference values. The program performs the following main tasks: graphical display of the distribution of reference values, identification or elimination of outliers, testing of the fit of the distribution to Gaussian shape (coefficients of skewness and kurtosis, Anderson-Darling's test,

Helge Erik Solberg

1995-01-01

161

A Competency-Based Clinical Chemistry Course for the Associate Degree Medical Laboratory Technician Graduate in a Medical Technology Baccalaureate Program.  

ERIC Educational Resources Information Center

|Presented is a project that developed a competency-based clinical chemistry course for associate degree medical laboratory technicians (MLT) in a medical technology (MT) baccalaureate program. Content of the course was based upon competencies expected of medical technologists at career-entry as defined in the statements adopted in 1976 by the…

Buccelli, Pamela

162

Population-Based Genomewide Genetic Analysis of Common Clinical Chemistry Analytes  

Microsoft Academic Search

BACKGROUND: Recent technologies enable genetic asso- ciation studies of common clinical analytes on a genomewide basis in populations numbering thou- sands of individuals. The first publications using these technologies are already revealing novel biological functions for both genic and nongenic loci, and are promising to transform knowledge about the biologi- cal networks underlying disease pathophysiology. Theseearlystudieshavealsoledtodevelopmentofaset of principles for conducting

Daniel I. Chasman; Guillaume Pare; Paul M Ridker

163

Practical and theoretical implications of target values in clinical chemistry: a QC approach  

Microsoft Academic Search

The automation of test validation procedures in a routine clinical laboratory by integrating artificial intelligence with the lab information system (LIS) is the focus of this study. Test validation consists of three “layers”: (1) acceptance of patient results based on technical criteria (“technical validation”), (2) acceptance of patient results based on internal quality control (QC) results (“QC validation”) and (3)

Viviane Van Hoof; Hugo Cluckers; Vera Verhaeghe; Marleen Truyens; An Hennebel

2004-01-01

164

Investigation on Plasmodium falciparum and Plasmodium vivax infection influencing host haematological factors in tribal dominant and malaria endemic population of Jharkhand.  

PubMed

The study was undertaken to elucidate the association of host haematological and biochemical indices in Plasmodium falciparum and Plasmodium vivax malaria in order to explore whether these parameters are unique to disease or act as a potential diagnostic marker. Haematological and biochemical parameters in 106 malarial patients and 33 healthy subjects were evaluated. Following parameters were significantly lower in all infection types (P. vivax, P. falciparum and mixed infection); haemoglobin, blood sugar, PCV and blood urea, while ESR is significantly higher in all types of infection whereas serum bilirubin and creatinine are significantly higher except mixed and vivax infection, respectively. Interestingly, parasitaemia, temperature and age are significantly correlated with blood urea, blood sugar and ESR respectively in vivax infection whereas parasitaemia with PCV and blood sugar and age with PCV in falciparum infection. Malaria infected subjects exhibited alterations in some haematological parameters with low haemoglobin, blood sugar and PCV whereas elevated ESR and serum bilirubin being the important findings observed in our study. These evaluations could be considered to be reliable clinical and biochemical markers for promising diagnostic potential during clinical malarial infection in combination with other genetic and classical microscopic parameters. Haematological evaluation could help in prompt and accurate diagnosis and prevent disease progression by facilitating physicians in clinical correlation for better drug regime. PMID:23961236

Hussain, Mohammad Mobassir; Sohail, Mohammad; Abhishek, Kumar; Raziuddin, Mohammad

2013-02-01

165

The clinical chemistry and immunology of long-duration space missions.  

PubMed

Clinical laboratory diagnostic capabilities are needed to guide health and medical care of astronauts during long-duration space missions. Clinical laboratory diagnostics, as defined for medical care on Earth, offers a model for space capabilities. Interpretation of laboratory results for health and medical care of humans in space requires knowledge of specific physiological adaptations that occur, primarily because of the absence of gravity, and how these adaptations affect reference values. Limited data from American and Russian missions have indicated shifts of intra- and extracellular fluids and electrolytes, changes in hormone concentrations related to fluid shifts and stresses of the missions, reductions in bone and muscle mass, and a blunting of the cellular immune response. These changes could increase susceptibility to space-related illness or injury during a mission and after return to Earth. We review physiological adaptations and the risk of medical problems that occur during space missions. We describe the need for laboratory diagnostics as a part of health and medical care in space, and how this capability might be delivered. PMID:8419055

Wu, A H; Taylor, G R; Graham, G A; McKinley, B A

1993-01-01

166

Coordination chemistry may explain pharmacokinetics and clinical response of vanadyl sulfate in type 2 diabetic patients.  

PubMed

Vanadium, abbreviated V, is an early transition metal that readily forms coordination complexes with a variety of biological products such as proteins, metabolites, membranes and other structures. The formation of coordination complexes stabilizes metal ions, which in turn impacts the biodistribution of the metal. To understand the biodistribution of V, V in oxidation state iv in the form of vanadyl sulfate (25, 50, 100 mg V daily) was given orally for 6 weeks to 16 persons with type 2 diabetes. Elemental V was determined using Graphite Furnas Atomic Absorption Spectrometry against known concentrations of V in serum, blood or urine. Peak serum V levels were 15.4 ± 6.5, 81.7 ± 40 and 319 ± 268 ng ml(-1) respectively, and mean peak serum V was positively correlated with dose administered (r = 0.992, p = 0.079), although large inter-individual variability was found. Total serum V concentration distribution fit a one compartment open model with a first order rate constant for excretion with mean half times of 4.7 ± 1.6 days and 4.6 ± 2.5 days for the 50 and 100 mg V dose groups respectively. At steady state, 24 hour urinary V output was 0.18 ± 0.24 and 0.97 ± 0.84 mg in the 50 and 100 mg V groups respectively, consistent with absorption of 1 percent or less of the administered dose. Peak V in blood and serum were positively correlated (r = 0.971, p < 0.0005). The serum to blood V ratio for the patients receiving 100 mg V was 1.7 ± 0.45. Regression analysis showed that glycohemoglobin was a negative predictor of the natural log(ln) peak serum V (R(2) = 0.40, p = 0.009) and a positive predictor of the euglycemic-hyperinsulinemic clamp results at high insulin values (R(2) = 0.39, p = 0.010). Insulin sensitivity measured by euglycemic-hyperinsulinemic clamp was not significantly correlated with ln peak serum V. Globulin and glycohemoglobin levels taken together were negative predictors of fasting blood glucose (R(2) = 0.49, p = 0.013). Although V accumulation in serum was dose-dependent, no correlation between total serum V concentration and the insulin-like response was found in this first attempt to correlate anti-diabetic activity with total serum V. This study suggests that V pools other than total serum V are likely related to the insulin-like effect of this metal. These results, obtained in diabetic patients, document the need for consideration of the coordination chemistry of metabolites and proteins with vanadium in anti-diabetic vanadium complexes. PMID:23982218

Willsky, Gail R; Halvorsen, Katherine; Godzala Iii, Michael E; Chi, Lai-Har; Most, Mathew J; Kaszynski, Peter; Crans, Debbie C; Goldfine, Allison B; Kostyniak, Paul J

2013-10-23

167

The future of hospital laboratories. Position statement from the Royal Belgian Society of Clinical Chemistry (RBSCC).  

PubMed

To face the economic pressures arising from the current socio-economic conjuncture, hospital laboratories are endangered by an increasing trend towards the outsourcing of clinical laboratory tests to external (mega-) laboratories. This should allow hospitals to meet their economic requirements, but with an increased risk of loss of medical quality and, mid- to long-term, loss of cost effectiveness of healthcare at the national level. To anticipate current developments (economical and technological) that inevitably will affect the future of laboratory medicine, hospital laboratories should be proactive and enhance efficiency, reduce costs by consolidation, integrate into regional networks, and form alliances or partnerships. To create additional value, the core competency of laboratory professionals must be refocused to provide medical knowledge services (consultative support to clinicians) related to in vitro diagnostic testing. To integrate cost-efficiency with medical quality, implementation of a matricial organization - operational vs. biomedical level - could be an interesting approach. This integrated structure should create total quality of laboratory testing, managing the entire medical diagnostic cycle from the pre-preanalytical to post-postanalytical phase. PMID:19663541

Langlois, Michel R; Wallemacq, Pierre

2009-01-01

168

International Federation of Clinical Chemistry standardization project for the measurement of lipoprotein(a). Phase I. Evaluation of the analytical performance of lipoprotein(a) assay systems and commercial calibrators  

Microsoft Academic Search

A secondary reference material for lipoprotein(a) is required to standardize the measurement of lipopro- tein(a) in clinical laboratories worldwide. Towards this aim, the International Federation of Clinical Chemistry Working Group for the Standardization of Lipopro- tein(a) Assays has initiated a standardization project involving a total of 33 diagnostic company and clinical chemistry laboratories from 12 countries. In Phase 1, the

Jillian R. Tate; Nader Rifai; Kare Berg; Remy Couderc; Francesco Dati; Gert M. Kostner; Ikunosuke Sakurabayashi; Armin Steinmetz

169

Haematological parameters as bioindicators of insecticide exposure in teleosts  

Microsoft Academic Search

Haematological parameters, such as erythrocyte and leucocyte count, erythrocyte indices and thrombocyte number vis-a-vis coagulation\\u000a of blood has been considered bioindicators of toxicosis in fish following exposure to organochlorine, organophosphate, carbamate\\u000a and pyrethroid insecticides. This review deals with the effects of insecticides on the morphology of red blood cells, total\\u000a erythrocyte count, haemoglobin content, haematocrit, mean corpuscular volume, mean corpuscular

Narendra Nath Singh; Anil Kumar Srivastava

2010-01-01

170

Haematological changes of cats with Toxoplasma gondii -specific antibodies  

Microsoft Academic Search

The purpose of this study is to access the usefulness of haematological parameters in diagnosing of chronic toxoplasmosis\\u000a in cats. Antibodies of Toxoplasma gondii were detected in 50 cats using an enzyme-linked immunosorbent assay. Immunoglobulin M (IgM) and immunoglobulin G (IgG) were\\u000a measured with the cutoff point for T. gondii considered to be 1:64. Fifteen (30%) of the cats were

S. Javadi; S. Asri Rezaei; H. Tajik; M. Hadian; F. Shokouhi

2010-01-01

171

Modern molecular diagnostics and the management of haematological malignancies.  

PubMed

The advent of molecular biological techniques has led to a radical improvement in the evaluation of haematological disorders and offers exciting future prospects. In particular it has led to the improved recognition of distinct clinicopathological entities defined by a combination of morphological, immunophenotypic and chromosomal features. Antibody-based techniques, namely immunophenotyping and immunocytochemical techniques, are essential to malignant haematological diagnosis. More novel is the diagnostic use of antibodies against novel fusion proteins, for example in AML, M3 and anaplastic lymphoma. PCR-based techniques allow the identification of genetic mutations and translocations including such common translocations as t(14;18), t(9;22), inv 16 and t(8;21). Fish-based techniques are greatly improving the ability to detect genetic abnormalities including those conditions with low cell proliferation and current research-based techniques include the combination of FISH with cell surface markers (FICTION), FIBRE FISH and Comparative Genomic Hybridization. Molecular techniques are essential in monitoring residual disease which is best illustrated in CML. The development of real-time automated PCR offers exciting prospects in this field. The increasing number of tests, the need for an integrated approach to diagnosis and the need for cost effectiveness indicate that such services should be provided by a specialized haematology laboratory. PMID:9681227

Morgan, G J; Pratt, G

1998-06-01

172

Effects of Aflatoxin on Some Haematological Parameters and Protective Effectiveness of Esterified Glucomannan in Merino Rams  

PubMed Central

The objective of the present study was to evaluate the toxic effects of aflatoxin on some hematological parameters and to determine the preventive effectiveness of added glucomannan. In the study, 32 Merino rams were used, and the rams were separated equally to four groups as control (C), glucomannan (G), glucomannan?+?aflatoxin (AG), and aflatoxin (A). Erythrocyte, leukocyte count, hemoglobin, and hematocrit levels were decreased in A group compared with the other groups, and there was a reduction in similar parameters in AG group compared to control values. On the other hand, these parameters were tended to increase in AG group compared to A group values. Aflatoxicosis caused the lymphocytopenia and monocytopenia but increased percentage of neutrophil counts. In conclusion, the results determined in the study might be important to demonstrate the effects of aflatoxicosis and glucomannan on some haematological parameters before the clinical symptoms appear.

Donmez, Nurcan; Donmez, H. H.; Keskin, E.; K?sadere, I.

2012-01-01

173

Biosensors in clinical chemistry  

Microsoft Academic Search

Biosensors are analytical devices composed of a recognition element of biological origin and a physico-chemical transducer. The biological element is capable of sensing the presence, activity or concentration of a chemical analyte in solution. The sensing takes place either as a binding event or a biocatalytical event. These interactions produce a measurable change in a solution property, which the transducer

Paul D'Orazio

2003-01-01

174

Exploring haematology nurses' perceptions of specialist education's contribution to care delivery and the development of expertise.  

PubMed

The role that educational preparation may play in the delivery of care and the development of expertise is a point of some debate [Manley, K., Garbett, R., 2000. Paying Peter and Paul reconciling concepts of expertise with competency for a clinical career structure. Journal of Clinical Nursing 9 (3), 347; King, L., Macleod Clark, J., 2002. Intuition and the development of expertise in surgical ward and intensive care nurses. Journal of Advanced Nursing 37(4), 322-329; Bonner, A., 2003. Recognition of expertise: an important concept in the acquisition of nephrology nursing expertise. Nursing & Health Sciences Journal 5, 123-131; Dunphy, B.C., Williamson, S.L., 2004. In pursuit of expertise. Advances in Health Sciences Education 9, 107-127]. Though education is a concept that may be universally valued, it may be more difficult to clearly discern the significance it has for practitioners who are developing their expertise. This research project employed an interpretive phenomenological design to explore the perceptions of specialist haematology nursing staff on the extent to which specialist education contributes to care delivery and the development of expert practice. A non-representative purposive sample of qualified nurses who had undertaken specialist education in haemopoiesis and work in specialist haematology participated in a focus group and semi-structured interviews. The report concludes that, for these specialist practitioners, specialist educational input had a beneficial impact on their levels of knowledge and confidence. Further to this, involvement in higher education had enabled them to become more active in the learning process. Perhaps the key finding of the study was the assertion by respondents that specialist educational input had enabled them to develop their specialist practice to a level that experience alone could not achieve. PMID:17126955

Atkinson, Joanne; Tawse, Stephen

2006-11-28

175

Comparison of performance of classical clinical chemistry analysers with test-strip devices (Reflotron) and those based on film technology (Vitros) in external quality assessment (EQA) surveys.  

PubMed

This article reports on the performance of two "dry" chemistry devices, (Reflotron, Roche Diagnostics and Vitros, Johnson & Johnson) and compared them with classical "wet" chemistry analysers in four commercially produced quality assessment samples (Roche PNU and PPU and Seronorm Human and Human High Controls) sent repeatedly over a 12-month observation period. Eleven analytes (including five enzymes) were studied, eight of which had target values set by reference method procedures. The results showed that both devices gave comparative results for the same sample sent in different EQA-surveys. Statistically significant differences which occurred were due to the high precision of measurement with a minimal shift in the measured concentrations. They had no clinical relevance in interpretation of results. Comparisons between "dry" and "wet" chemistry results for the same analyte were almost always statistically significantly different and often large enough to influence the clinical interpretation of results. Examples here were glucose and uric acid measured with the Reflotron and compared with other Roche devices (Cobas, Hitachi). The Vitros showed deviant values for urea and creatinine, when compared with other measuring devices using liquid reagents. Differences seen were constant over time, but must be seen in context with the matrices of the samples sent. The results show the long term stability of both reagents and test kits, a necessary prerequisite for long-term controlling of precision and indirectly accuracy of patient measurements. PMID:19097494

Wood, William Graham

2008-01-01

176

Invented fairy tales in groups with onco-haematological children.  

PubMed

BACKGROUND: The impact of an onco-haematological illness for children is a traumatic event that opens to pain, hospitalizations and interrupts the continuity of daily life. It is difficult for the child to make meaning, to share the pain or ask a question related to the illness because, often, the parents or doctors cannot find a way to communicate in a suitable way for the child who remains in a situation of 'unspoken', where, fear, anxiety and pain cannot find a space to express. METHODS: The present research-intervention uses the methodology of invented fairy tales in groups with onco-haematological children, in the hospital, in order to explore the organization of the meanings at the base of the tales co-constructed by the participants underlying weaknesses and strengths of the invented fairy tales in groups intervention. The invented fairy tales in groups is used as a tool, such as a play, to express, share and support the experience of the illness of children. Forty-nine children participated to the invented fairy tales in groups in an onco-haematological hospital. Within a quali-quantitative framework we performed a thematic analysis of elementary context, cluster analysis, on the fairy tales considered as a unique narrative corpus of the thought of the group. RESULTS: The analysis shows four thematic clusters: fantasy as search for a meaning, 29.71%, the group as a space for illusions, 27.90%, the illness as a family problem, 25.72%, anchoring reality, 16.67%. The results highlighted three main carriers of sense: the representation of illness/the relational world/the representation of the institution. CONCLUSIONS: The use of invented-fairy-tales groups allowed the onco-haematological children to tell and share the experience of illness through a different way, which let them express symbolically their pain. The invented fairy tale in groups becomes a mediator of psychic processes which offer new solutions while improving interpersonal relationships/communication between the participants in group. PMID:23672257

Margherita, G; Martino, M L; Recano, F; Camera, F

2013-05-14

177

Cavernosal arterial insufficiency is a major component of erectile dysfunction in some recipients of high-dose chemotherapy\\/chemo-radiotherapy for haematological malignancies  

Microsoft Academic Search

We studied 24 male patients aged 26–62 years (median 41) prospectively presenting over a 5 year period with clinical features of hypogonadism and erectile dysfunction (ED), who had been treated with autologous or allogeneic bone marrow\\/stem cell transplant for a variety of haematological malignancies and had received either high-dose chemotherapy or high-dose chemotherapy combined with total body irradiation (TBI). Ten

R Chatterjee; HO Andrews; HH McGarrigle; PD Kottaridis; WR Lees; S Mackinnon; DJ Ralph; AH Goldstone

2000-01-01

178

Development and application of biochemical and haematological reference intervals to identify unhealthy green sea turtles (Chelonia mydas).  

PubMed

Biochemical and haematological reference intervals (RIs) have been reported for sea turtles, but their value for ante-mortem disease diagnosis may be limited due to small sample sizes and outdated statistical analyses. In the present study, 290 green sea turtles (Chelonia mydas) were captured, clinically assessed and blood sampled. Of these, 211 were classified as 'clinically healthy' and 25 as 'clinically unhealthy'. RIs were estimated using data from the healthy turtles and compared with blood values from the unhealthy animals. All of the unhealthy animals had plasma biochemical and haematological values outside one or more RIs (albumin, 48% of unhealthy animals; alkaline phosphatase, 35%; aspartate transaminase, 13%; creatinine, 30%; globulin, 3%; glucose, 34%; lactic dehydrogenase, 26%; phosphorus, 22%; sodium, 13%; thrombocytes, 57%; and monocytes, 5%). Among small immature turtles, those with Chelonibia testudinaria plastron barnacle counts 20 were three times more likely to be unhealthy than those with no barnacles. In addition, small immature and mature turtles were more likely to be unhealthy than large immature turtles. PMID:19709912

Flint, Mark; Morton, John M; Limpus, Colin J; Patterson-Kane, Janet C; Murray, Peter J; Mills, Paul C

2009-08-25

179

Effect of long-term highly active antiretroviral therapy (HAART) on some haematological parameters of HIV-infected Nigerians  

Microsoft Academic Search

Haematologic abnormalities are among the most common manifestations of advanced HIV infection and AIDS. The aim of this case controlled study was to examine the long-term effect of HAART on the haematological profile of HIV-infected Nigerians. One hundred consecutive patients were randomized to take a fixed drug combination of stavudine, lamivudine and nevirapine. Haematological parameters were analyzed at baseline and

Osaro Erhabor; Emmanuel Kufre Uko; Osekhuiemen Adebayo Ejele; Confidence Wachukwu

180

Follow up study of haematological effects in workers exposed to 2-methoxyethanol  

PubMed Central

Aims: To examine the association between 2-methoxyethanol (2-ME) exposure and haematological effects, as well as the recovery from these haematological effects with continuous reduction in exposure to 2-ME. Methods: Twenty nine exposed and 90 non-exposed workers were recruited. Haematological parameters, eight hour full shift personal exposure to 2-ME, and urinary 2-methoxyacetic acid (MAA) were repeatedly measured in three consecutive surveys within six months. Results: Results of haematological examination in the first exposure survey showed that haemoglobin, packed cell volume, and red blood cell count in the male exposed workers were significantly lower than those in the comparison workers. The frequency of anaemia in the exposed group (42%) was significantly higher than that in the comparison group (3%). The haematological effects were significantly associated with the urinary MAA of exposed workers. The haematological effects had returned to normal in the first follow up survey 2.5 months later, when a reduction in 2-ME exposure was noted. Haematological results of the second follow up examination six months later remained normal. The mean airborne exposure of 2-ME in the three surveys dropped from 35.7 to 2.65, then to 0.55 ppm. The mean urinary MAA of exposed workers in the three surveys was reduced from 57.7 to 24.6, then to 13.5 mg/g creatinine (n = 29). The reduction in exposure through both inhalation and potential dermal contact with 2-ME might account for the haematological recovery. Conclusion: 2-ME is a haematological toxin which leads to anaemia in exposed workers. However, the toxic haematological effects of 2-ME persist for only a short period of time after cessation or reduction of exposure.

Shih, T; Hsieh, A; Chen, Y; Liao, G; Chen, C; Chou, J; Liou, S

2003-01-01

181

Haemodynamic and haematologic effects of Acanthaster planci venom in dogs.  

PubMed

This study was designed to examine haemodynamic and haematologic effects of the crown-of-thorns starfish venom (Acanthaster planci venom: APV) in dogs. Severe systemic hypotension, thrombocytopenia and leukopenia were induced by APV (1.0 mg protein/kg i.v.), followed by gradual return to the baseline level within 60 min. Hypotension was presumably caused by two factors: an early decrease in systemic vascular resistance and the large reduction in cardiac output due to reduced ventricular filling. Indomethacin, a cyclooxygenase inhibitor, remarkably suppressed systemic hypotension induced by APV. The peak reduction in systemic pressure was associated with concomitant rise of plasma 6-keto-PGF1 alpha, a major stable metabolite of prostacyclin. Thus, the hypotensive effect of APV may be caused primarily by prostacyclin and/or some vasodilating prostaglandins. In contrast, thrombocytopenia and leukopenia were not affected by cyclooxygenase inhibitor, 5-lipoxygenase inhibitor or platelet activating factor (PAF) receptor antagonist. When APV was administered repeatedly, tachyphylaxis was developed in haemodynamic effects, but not in haematologic effects. These findings suggest that APV-induced hypotensive effects may occur mainly through endogenous production of vasodilating prostaglandins including prostacyclin, although APV-induced thrombocytopenia and leukopenia may be caused by other mechanism(s) unrelated to arachidonate metabolites and/or PAF. PMID:7846692

Shiroma, N; Noguchi, K; Matsuzaki, T; Ojiri, Y; Hirayama, K; Sakanashi, M

1994-10-01

182

Long Non-Coding RNAs in Haematological Malignancies  

PubMed Central

Long non-coding RNAs (lncRNAs) are functional RNAs longer than 200 nucleotides in length. LncRNAs are as diverse as mRNAs and they normally share the same biosynthetic machinery based on RNA polymerase II, splicing and polyadenylation. However, lncRNAs have low coding potential. Compared to mRNAs, lncRNAs are preferentially nuclear, more tissue specific and expressed at lower levels. Most of the lncRNAs described to date modulate the expression of specific genes by guiding chromatin remodelling factors; inducing chromosomal loopings; affecting transcription, splicing, translation or mRNA stability; or serving as scaffolds for the organization of cellular structures. They can function in cis, cotranscriptionally, or in trans, acting as decoys, scaffolds or guides. These functions seem essential to allow cell differentiation and growth. In fact, many lncRNAs have been shown to exert oncogenic or tumor suppressor properties in several cancers including haematological malignancies. In this review, we summarize what is known about lncRNAs, the mechanisms for their regulation in cancer and their role in leukemogenesis, lymphomagenesis and hematopoiesis. Furthermore, we discuss the potential of lncRNAs in diagnosis, prognosis and therapy in cancer, with special attention to haematological malignancies.

Garitano-Trojaola, Andoni; Agirre, Xabier; Prosper, Felipe; Fortes, Puri

2013-01-01

183

Use of thrombin inhibitors ex vivo allows critical care clinical chemistry and hematology testing on common specimens  

Microsoft Academic Search

Objective: To evaluate the suitability of the thrombin inhibitors PPACK (D-phenylalanyl-L-prolyl-L-arginine chloromethylketone) or Argatroban for anticoagulation of blood prior to critical care testing of whole blood or plasma.Design and Methods: Initially we evaluated the effect of PPACK (0–200 ?M) or Argatroban (0–590 ?M) on serum glucose, urea, creatinine, calcium and electrolyte tests on two chemistry analyzers (Hitachi 717 and Ektachem

Andrew W. Lyon; Sheila Rutledge Harding; Duane Drobot; Martha E. Lyon

1997-01-01

184

[The spleen in non-malignant haematological disorders].  

PubMed

The spleen functions as a filter of the circulating blood, removing aging or abnormal red blood cells, intraerythrocyte inclusions as well as foreign particals. As the spleen is composed of lymphocytic tissue, circulatory elements and mononuclear phagocytic cells it plays an important role in the nonspecific as well as the specific immune response. Additionally, the spleen serves as a reservoir for circulating blood cells, especially platelet sequestration by the spleen is well do cumented. The spleen produces blood cells during fetal development and in certain haematological disorders such as myelofibrosis. The destruction of red blood cells within the splenic cords releases iron in the circulation, which is recycled and used to manufacture new erythrocytes in the bone marrow. In several non-malignant haematological disorders antibody-coated cells are cleared from the circulation by phagocytic cells of the spleen. This involves erythrocytes in autoimmunhaemolytic anaemias, platelets in immunthrombocytopenia and neutrophils in Felty syndrome. In hereditary spherocytosis the spleen destroys the resulting defective, spherical red cells. In pyruvate kinase deficiency impaired production of adenosine triphosphate leads to destruction of red blood cells in the spleen or in the liver. In sickle cell anaemia the defective erythrocytes cause sludging and thrombosis in small vessels with infarcts for instance in the spleen, which over time can result in autosplenectomy. In thalassaemia major abnormal haemoglobin forms protein precipitates in the red cells with development of a severe hypochromic anaemia with haemolysis and intramedullary inef fective erythropoiesis. Therapeutic splenectomy can be an option in all of these mentioned non-malignant haematological disorders. The rationale and the pathophysiology of its role in thrombotic thrombocytopenic purpura is probably at least well understood. The use of new and effective drugs such as the monoclonal antibody rituximab or thrombopoietin agonists in immunthrombocytopenia as well as the option of a laparoscopic rather than an open splenectomy have changed our view on benefits and risks of splenectomy in recent years. To minimize these risks such as overwhelming postsplenectomy infection or abdominal vein thrombosis, prophylactic vaccination, postoperative thromboembolism prophylaxis and patient education is mandatory. PMID:23454562

Rüfer, Axel; Wuillemin, Walter A

2013-03-01

185

Oncogenic properties of sphingosine kinases in haematological malignancies.  

PubMed

The sphingosine kinases (SphKs) have relatively recently been implicated in contributing to malignant cellular processes with particular interest in the oncogenic properties of SPHK1. Whilst SPHK1 has received considerable attention as a putative oncoprotein, SPHK2 has been much more difficult to study, with often conflicting data surrounding its role in cancer. Initial studies focused on non-haemopoietic malignancies, however a growing body of literature on the role of sphingolipid metabolism in haemopoietic malignancies is now emerging. This review provides an overview of the current state of knowledge of the SphKs and the bioactive lipid sphingosine 1-phosphate (S1P), the product of the reaction they catalyse. It then reviews the current literature regarding the roles of S1P and the SphKs in haemopoietic malignancies and discusses the compounds currently available that modulate sphingolipid metabolism and their potential and shortcomings as therapeutic agents for the treatment of haematological malignancies. PMID:23521541

Wallington-Beddoe, Craig T; Bradstock, Kenneth F; Bendall, Linda J

2013-03-25

186

Targeting Metabolism and Autophagy in the Context of Haematologic Malignancies  

PubMed Central

Autophagy is a cellular process that maintains the homeostasis of the normal cell. It not only allows for cell survival in times of metabolic stress with nutrient recycling but also is able to lead to cell death when required. During malignant transformation the cell is able to proliferate and survive. This is due to altered cell metabolism and the presence of altered genetic changes that maintain the cell survival. Metabolism was considered an innocent bystander that was a consequence of the increased nutrient requirement for the survival and proliferation of haematological malignancies. The interdependency of metabolism and cellular mechanisms such as autophagy are becoming more evident and important. This interdependence contributes to increased cancer progression and drug resistance. In this paper we aim to discuss autophagy, how it pertains to metabolism in the context of hematologic malignancies, and the implications for therapy.

Banerji, Versha; Gibson, Spencer B.

2012-01-01

187

Haematological and haemorheological changes associated with cigarette smoking.  

PubMed Central

A prospective study was undertaken to establish the haematological and haemorheological changes associated with long standing cigarette smoking in 20 heavy smokers and to assess whether any such changes were reversible after smoking was stopped. Highly significant differences were observed in whole blood viscosity, plasma viscosity, plasma fibrinogen concentrations, packed cell volume, and carboxyhaemoglobin concentrations between smokers and non-smokers. Ten of the subjects were followed up for two weeks after stopping smoking by which time whole blood viscosity and carboxyhaemoglobin concentrations had improved significantly and indications of improvement could be seen in all other measurements. Two of these subjects were further followed up for two months when all the measured variables were comparable with those in non-smokers. No correlation could be established between carboxyhaemoglobin concentration and any of the other variables. Although patients' compliance may be difficult to obtain, further prospective studies would be required to confirm our findings.

Galea, G; Davidson, R J

1985-01-01

188

Green Chemistry  

NSDL National Science Digital Library

This special feature page from the American Chemical Society (ACS) showcases the up-and-coming field of "green chemistry," that is, the development of chemical products and processes that eliminate or reduce the use and generation of hazardous substances. A list of principles behind green chemistry, a searchable bibliography of green chemistry references, green chemistry links (including conferences), and an online preview of the ACS-published book Real-World Cases in Green Chemistry are all found at the site. Five video clips on green chemistry from the standpoint of academia, industry, and small business are also featured (Windows Media Player). This page comes from ACS's Green Chemistry Project, a three-year educational project to develop and disseminate green chemistry educational materials for graduate and undergraduate chemistry students. Check back often for updates.

2002-01-01

189

Parameters of disease progression in long-term experimental feline retrovirus (feline immunodeficiency virus and feline leukemia virus) infections: hematology, clinical chemistry, and lymphocyte subsets.  

PubMed Central

After several years of latency, feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) cause fatal disease in the cat. The aim of this study was to determine laboratory parameters characteristic of disease progression which would allow a better description of the asymptomatic phase and a better understanding of the pathogenesis of the two infections. Therefore, experimentally infected cats (FIV and/or FeLV positive) and control animals were observed over a period of 6.5 years under identical conditions. Blood samples were analyzed for the following: complete hematology, clinical chemistry, serum protein electrophoresis, and determination of CD4+ and CD8+ lymphocyte subsets. The following hematological and clinical chemistry parameters were markedly changed in the FIV-infected animals from month 9 onwards: glucose, serum protein, gamma globulins, sodium, urea, phosphorus, lipase, cholesterol, and triglyceride. In FeLV infection, the markedly changed parameters were mean corpuscular volume, mean corpuscular hemoglobin, aspartate aminotransferase, and urea. In contrast to reports of field studies, neither FIV-positive nor FeLV-positive animals developed persistent leukopenia, lymphopenia, or neutropenia. A significant decrease was found in the CD4+/CD8+ ratio in FIV-positive and FIV-FeLV-positive animals mainly due to loss of CD4+ lymphocytes. In FeLV-positive cats, both CD4+ and, to a lesser degree, CD8+ lymphocytes were decreased in long-term infection. The changes in FIV infection may reflect subclinical kidney dysfunction, changes in energy and lipid metabolism, and transient activation of the humoral immune response as described for human immunodeficiency virus (HIV) infections. The changes in FeLV infection may also reflect subclinical kidney dysfunction and, in addition, changes in erythrocyte and immune function of the animals. No severe clinical signs were observed in the FIV-positive cats, while FeLV had a severe influence on the life expectancy of persistently positive cats. In conclusion, several parameters of clinical chemistry and hematology were changed in FIV and FeLV infection. Monitoring of these parameters may prove useful for the evaluation of candidate FIV vaccines and antiretroviral drugs in cats. The many parallels between laboratory parameters in FIV and HIV infection further support the importance of FIV as a model for HIV.

Hofmann-Lehmann, R; Holznagel, E; Ossent, P; Lutz, H

1997-01-01

190

Serum galactomannan assay for the diagnosis of invasive aspergillosis in children with haematological malignancies.  

PubMed

Diagnostic efficacy of Galactomannan (GM) assay for invasive aspergillosis (IA) is variably reported. Data from developing countries are scant. Children with haematological malignancies and fever were enrolled prospectively. Blood sample for GM was drawn on the day of admission; levels were measured with Platellia Aspergillus enzyme immunoassay. Diagnostic criteria were adapted from EORTC-MSG-2002. Proven, probable and possible episodes were considered as the disease group. One hundred febrile episodes in 78 patients were evaluated. The mean age was 6.1?years. Majority (75%) episodes were in patients with acute lymphoblastic leukaemia. One episode each was diagnosed with proven and probable IA, while 23 were diagnosed with possible IA. Best results were obtained with a cut-off value of 1.0, with sensitivity, specificity, positive and negative predictive value of 60%, 93%, 75 and 87 respectively. The sensitivity dropped to 40%, at cut-off value of 1.5 and specificity was 38%, at a cut-off of 0.5. A higher value of GM correlated with pulmonary nodules (P?=?0.037) and mortality (P?=?0.001). GM assay is adjunctive to clinical/radiological evidence. A negative GM assay may not reassure the physician against the use of amphotericin in patients with febrile neutropenia, as it does not exclude the diagnosis of clinically relevant other fungal infections, particular mucormycosis. PMID:23369047

Jha, Ajaya K; Bansal, Deepak; Chakrabarti, Arunaloke; Shivaprakash, M R; Trehan, Amita; Marwaha, Ram K

2013-02-01

191

CLINICAL BIOCHEMISTRY  

EPA Science Inventory

Assessment of the health status of animals through measurement of cellular, biochemical, and macromolecular constituents in blood, secretions, and excretions has been variously referred to as clinical chemistry, clinical biochemistry, or clinical pathology. he genesis of this dis...

192

Haematological health assessment in a passerine with extremely high proportion of basophils in peripheral blood  

Microsoft Academic Search

Haematological methods are widely utilised among avian ecologists as a means for individual health assessment. However, the\\u000a technical simplicity of some of the tests may easily lead to oversimplification of the evaluation. Here, we show in the Scarlet\\u000a Rosefinch (Carpodacus erythrinus) that haematological parameters other than the widely used heterophil\\/lymphocyte (H\\/L) ratio may be important to investigate.\\u000a We give the

Michal Vinkler; Jan Schnitzer; Pavel Munclinger; Jan Votýpka; Tomáš Albrecht

2010-01-01

193

Effect of altitude training on haematological variables in women volleyball players  

Microsoft Academic Search

BackgroundAthletes from many endurance disciplines include altitude training in their yearly training programme. In volleyball, it is necessary to clarify the haematological benefits and improvement in sports performance.ObjectiveTo investigate the influence of moderate altitude on haematological variables in a women's volleyball team (Honor Division – Spain).Design and settingWe assessed 14 elite female volleyball players for two weeks in normoxia (640

B Cueto-Martín; J C de la Cruz-Márquez; J C de la Cruz-Campos; A de la Cruz-Campos

2011-01-01

194

Chemistry Notes.  

ERIC Educational Resources Information Center

Presents background information, laboratory procedures, classroom materials/activities, and chemistry experiments. Topics include sublimation, electronegativity, electrolysis, experimental aspects of strontianite, halide test, evaluation of present and future computer programs in chemistry, formula building, care of glass/saturated calomel…

School Science Review, 1983

1983-01-01

195

Chemistry Notes.  

ERIC Educational Resources Information Center

|Presents background information, laboratory procedures, classroom materials/activities, and chemistry experiments. Topics include sublimation, electronegativity, electrolysis, experimental aspects of strontianite, halide test, evaluation of present and future computer programs in chemistry, formula building, care of glass/saturated calomel…

School Science Review, 1983

1983-01-01

196

Organic Chemistry  

NSDL National Science Digital Library

R. H. Logan, an chemistry instructor at North Lake College, created this introduction to organic chemistry. The introduction covers a eight types of organic compounds, including Alkanes, Alkyl Halides, and Acyl Compounds (forthcoming); Conformational Analysis and Stereoisomerism; and Instrumental Analysis of Organic Compounds, as well an extensive lesson in general chemistry.

197

Forensic Chemistry  

Microsoft Academic Search

“Forensic chemistry” is a broad term that, if taken literally, would encompass most of the functions within a crime laboratory.\\u000a Techniques used in forensic chemistry are also used by the toxicology and trace analysis sections. However, forensic chemistry\\u000a generally refers to controlled substance or drug analysis.

Donnell Christian

198

Forensic Chemistry  

Microsoft Academic Search

Forensic chemistry is unique among chemical sciences in that its research, practice, and presentation must meet the needs of both the scientific and the legal communities. As such, forensic chemistry research is applied and derivative by nature and design, and it emphasizes metrology (the science of measurement) and validation. Forensic chemistry has moved away from its analytical roots and is

Suzanne Bell

2009-01-01

199

Chemistry Notes.  

ERIC Educational Resources Information Center

|Presents background information, laboratory procedures, classroom materials/activities, and experiments for chemistry. Topics include superheavy elements, polarizing power and chemistry of alkali metals, particulate carbon from combustion, tips for the chemistry laboratory, interesting/colorful experiments, behavior of bismuth (III) iodine, and…

School Science Review, 1982

1982-01-01

200

Hematology and clinical chemistry reference ranges for clinically normal, captive New Guinea snapping turtle (Elseya novaeguineae) and the effects of temperature, sex, and sample type.  

PubMed

Median values and confidence intervals for hematology and serum and plasma chemistry parameters were established for 29 male and female healthy New Guinea snapping turtles (Elseya novaeguineae) held at 24.5 degrees C and 30.0 degrees C. Creatine kinase, albumin, potassium, and phosphorus values were significantly higher at 24.5 degrees C than at 30.0 degrees C. Glucose, alkaline phosphatase, aspartate transaminase, alanine aminotransferase, total carbon dioxide, and chloride values were significantly higher at 30.0 degrees C than at 24.5 degrees C. Cholesterol and calcium values were significantly higher in females than in males. Hemoglobin, packed cell volume, and bilirubin were significantly higher in males than in females, and bile acid values were significantly higher in serum than in plasma. PMID:9523632

Anderson, N L; Wack, R F; Hatcher, R

1997-12-01

201

CLUSTER CHEMISTRY  

SciTech Connect

Metal cluster chemistry is one of the most rapidly developing areas of inorganic and organometallic chemistry. Prior to 1960 only a few metal clusters were well characterized. However, shortly after the early development of boron cluster chemistry, the field of metal cluster chemistry began to grow at a very rapid rate and a structural and a qualitative theoretical understanding of clusters came quickly. Analyzed here is the chemistry and the general significance of clusters with particular emphasis on the cluster research within my group. The importance of coordinately unsaturated, very reactive metal clusters is the major subject of discussion.

Muetterties, Earl L.

1980-05-01

202

Forensic Chemistry  

NASA Astrophysics Data System (ADS)

Forensic chemistry is unique among chemical sciences in that its research, practice, and presentation must meet the needs of both the scientific and the legal communities. As such, forensic chemistry research is applied and derivative by nature and design, and it emphasizes metrology (the science of measurement) and validation. Forensic chemistry has moved away from its analytical roots and is incorporating a broader spectrum of chemical sciences. Existing forensic practices are being revisited as the purview of forensic chemistry extends outward from drug analysis and toxicology into such diverse areas as combustion chemistry, materials science, and pattern evidence.

Bell, Suzanne

2009-07-01

203

Effect of sample volume on yield of positive blood cultures from adult patients with haematological malignancy.  

PubMed Central

Six hundred and sixteen blood samples from patients with haematological malignancy were each distributed equally among three identical cells in a Malthus Microbiological Growth Analyser. The mean (SD) volumes inoculated into sets in which one, two, or three of the three bottles were positive were 37.7 (10.1) ml, 37.4 (12.9) ml, and 37.7 (10.5) ml, respectively. Overall, clinically important organisms were isolated from one bottle only with 18 cultures, from two bottles only with 19 cultures, and from all three bottles in a set with 104 cultures. If the yield from a single bottle inoculated with a mean volume of 12.6 ml blood is taken as 100%, the yield from 25.2 ml in two bottles was 110.7% and the yield from 37.7 ml in three bottles was 115.6%. The increased yield from increased volume was considerably lower than that reported from unselected groups of patients, which suggests that the magnitude of bacteriaemia is greater in patients with neutropenia. The isolation of infecting organisms from the blood of patients with neutropenia is, however, particularly important in directing chemotherapy and consequently 45 ml blood samples from these patients continue to be requested.

Brown, D F; Warren, R E

1990-01-01

204

Reversible phosphorylation in haematological malignancies: potential role for protein tyrosine phosphatases in treatment?  

PubMed

Most aspects of leukocyte physiology are under the control of reversible tyrosine phosphorylation. It is clear that excessive phosphorylation of signal transduction elements is a pivotal element of many different pathologies including haematological malignancies and accordingly, strategies that target such phosphorylation have clinically been proven highly successful for treatment of multiple types of leukemias and lymphomas. Cellular phosphorylation status is dependent on the resultant activity of kinases and phosphatases. The cell biology of the former is now well understood; for most cellular phosphoproteins we now know the kinases responsible for their phosphorylation and we understand the principles of their aberrant activity in disease. With respect to phosphatases, however, our knowledge is much patchier. Although the sequences of whole genomes allow us to identify phosphatases using in silico methodology, whereas transcription profiling allows us to understand how phosphatase expression is regulated during disease, most functional questions as to substrate specificity, dynamic regulation of phosphatase activity and potential for therapeutic intervention are still to a large degree open. Nevertheless, recent studies have allowed us to make meaningful statements on the role of tyrosine phosphatase activity in the three major signaling pathways that are commonly affected in leukemias, i.e. the Ras-Raf-ERK1/2, the Jak-STAT and the PI3K-PKB-mTOR pathways. Lessons learned from these pathways may well be applicable elsewhere in leukocyte biology as well. PMID:20659529

Ruela-de-Sousa, Roberta R; Queiroz, Karla C S; Peppelenbosch, Maikel P; Fuhler, Gwenny M

2010-07-24

205

Thrombopoietin-receptor agonists in haematological disorders: the Danish experience.  

PubMed

The objective of this study was to investigate the use of thrombopoietin-receptor agonists (TPO-ra) in patients with refractory primary immune thrombocytopenia (ITP) as well as off-label use of TPO-ra in Danish haematology departments. Hospital medical records from 32 of the 39 patients having received TPO-ra from 2009 to 1 May 2011 were available for data collection and included in the study. Of these patients, 15 received TPO-ra for refractory primary ITP, 7 for secondary ITP (chronic lymphatic leukaemia, systemic lupus erythematosus, Evans syndrome, human immunodeficiency virus and celiac disease) and 10 were treated for non-ITP (chemotherapy-induced, acute myeloid leukaemia, myelodysplastic syndrome, hereditary spherocytosis and suspected chemically induced thrombocytopenia). Initial response to TPO-ra defined as platelet counts >30 × 10(9)/l after 4 weeks of treatment was found in 59% of primary ITP patients, 57% of patients with secondary ITP and 40% of patients with non-ITP. There were four deaths in the cohort, three of which were related to pre-existing medical conditions. Otherwise adverse effects were in general mild. This Danish retrospective registration study has demonstrated that in the off-protocol setting, the use of TPO-ra is associated with response rates largely similar to those seen in previous protocol-monitored studies and no new adverse events were reported. PMID:22185370

Gudbrandsdottir, Sif; Frederiksen, Henrik; Hasselbalch, Hans

2011-12-20

206

Predictors of sudden death and death from pump failure in congestive heart failure are different. Analysis of 24 h Holter monitoring, clinical variables, blood chemistry, exercise test and radionuclide angiography  

Microsoft Academic Search

One hundred and ninety consecutive patients discharged with congestive heart failure were examined with clinical evaluation, blood chemistry, 24 h Holter monitoring, exercise test and radionuclide angiography. Median left ventricular ejection fraction was 0.30, 46% were in New York Heart Association class II and 44% in III. Total mortality after 1 year was 21%, after 2 years 32%. Of 60

Bente Kühn Madsen; Verner Rasmussen; Jørgen Fischer Hansen

1997-01-01

207

Doing Chemistry  

NSDL National Science Digital Library

This website includes over 150 chemistry experiments in the following areas: Atomic Structure, Bonding, Chemical Reactions, Colligative Properties, Condensed States, Electrochemistry, Equilibrium Gases, Instrumentation, Limiting Reactant and more.

Brooks, David W.

2010-05-25

208

Chemistry Notes.  

ERIC Educational Resources Information Center

|Outlines laboratory procedures, demonstrations, teaching suggestions, and content information related to chemistry. Topics include polarizing power; calorimetry and momentum; microcomputers in school chemistry; a constant-volume dispenser for liquids, floating magnets, and crystal lattices; preparation of chromium; and solvent polarity and…

School Science Review, 1981

1981-01-01

209

Haematological parameters in a neotropical fish, Corydoras paleatus (Jenyns, 1842) (Pisces, Callichthyidae), captured from pristine and polluted water  

Microsoft Academic Search

We report normal ranges of haematological indices in healthy Corydoras paleatus from an unpolluted area. Haematological parameters studied include: erythrocyte counts (Er), haematocrit (Ht), haemoglobin concentration (Hb), mean cell volume (MCV), mean cell haemoglobin (MCH) and mean cell haemoglobin concentration (MCHC). Normal red blood parameters did not change according to maturation stages, sex or seasons. Then, we compared them with

Jimena Cazenave; Daniel Alberto Wunderlin; Andrea Cecilia Hued

2005-01-01

210

Haematological and Biochemical Values or the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni)  

Microsoft Academic Search

Va h a I a 1., F. K d e and O. A. R Y d e r: Haematological and Biochemical Values of the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni). Acta vet. Brno, 63,1994: 99-102. Haematological and biochemical blood and blood serum values obtained from 7 northern white rhinoceroses (Ceratotherium simum cottoni) kept in

J. Váhala; V. Kaše; O. A. Ryder

1994-01-01

211

Visualising the Cross-Level Relationships between Pathological and Physiological Processes and Gene Expression: Analyses of Haematological Diseases  

PubMed Central

The understanding of pathological processes is based on the comparison between physiological and pathological conditions, and transcriptomic analysis has been extensively applied to various diseases for this purpose. However, the way in which the transcriptomic data of pathological cells relate to the transcriptomes of normal cellular counterparts has not been fully explored, and may provide new and unbiased insights into the mechanisms of these diseases. To achieve this, it is necessary to develop a method to simultaneously analyse components across different levels, namely genes, normal cells, and diseases. Here we propose a multidimensional method that visualises the cross-level relationships between these components at three different levels based on transcriptomic data of physiological and pathological processes, by adapting Canonical Correspondence Analysis, which was developed in ecology and sociology, to microarray data (CCA on Microarray data, CCAM). Using CCAM, we have analysed transcriptomes of haematological disorders and those of normal haematopoietic cell differentiation. First, by analysing leukaemia data, CCAM successfully visualised known relationships between leukaemia subtypes and cellular differentiation, and their characteristic genes, which confirmed the relevance of CCAM. Next, by analysing transcriptomes of myelodysplastic syndromes (MDS), we have shown that CCAM was effective in both generating and testing hypotheses. CCAM showed that among MDS patients, high-risk patients had transcriptomes that were more similar to those of both haematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors (MEP) than low-risk patients, and provided a prognostic model. Collectively, CCAM reveals hidden relationships between pathological and physiological processes and gene expression, providing meaningful clinical insights into haematological diseases, and these could not be revealed by other univariate and multivariate methods. Furthermore, CCAM was effective in identifying candidate genes that are correlated with cellular phenotypes of interest. We expect that CCAM will benefit a wide range of medical fields.

Ono, Masahiro; Tanaka, Reiko J.; Kano, Manabu; Sugiman, Toshio

2013-01-01

212

Catastrophic bleeds during end-of-life care in haematology: controversies from Australian research  

Microsoft Academic Search

Goals of work  This article seeks to address the lack of information in the literature, by providing preliminary findings on the experience\\u000a of managing catastrophic bleeds for haematology patients, from the perspective of health professionals involved in the care\\u000a of such patients and their families.\\u000a \\u000a \\u000a \\u000a Materials and methods  Insights from qualitative interviews with seventeen (n?=?17) haematological nurses, four (n?=?4) palliative care nurses

Pam McGrath; Michael Leahy

2009-01-01

213

[Clinical signs and changes in serum and tissue chemistry in rats treated with vitamin D3 (calciferol)].  

PubMed

In the present work the effect of subcutaneous administration of 250, 500 and 750 microg (10.000, 20.000 and 30.000 IU, respectively) of vitamin D3 (calciferol) daily for eight days, on serum concentrations of vitamin D3 and 25-hydroxyvitamin D3 (25-OH-D3) and on serum and tissue concentrations of Ca, Zn, Cu and Fe in 45 white male Wistar rats, aged 12 weeks and weighing 180-200 g, have been studied. The group control was integrated by 15 healthy rats with similar characteristics (strain, gender, age and weight) that treated animals. Administration of high doses of calciferol produced a hypervitaminosis D characterized by a significant (p < 0.05) increase in serum vitamin D3 and 25-OH-D3, diverse clinical signs (such as, anorexia, marked loss of body weight, bloody diarrhea, bilateral conjunctivitis, and death), hypercalcemia, hypozincaemia, hypercupremia, hypoferraemia and an alteration in the tissue distribution of Ca, Zn, Cu and Fe as compared with untreated controls. Hypercalcemia and inflammation are prominent findings in hypervitaminosis D. Inflammation or infection induce systemic changes, collectively known as the acute phase response. Among the varied alterations that together produce this response are hypoferraemia, hypozincaemia and hypercupremia. It is likely that these responses are mediated, in part, by production and release of cytokines such as interleukin 1, interferons (IFN-alpha), interleukin 6 (11-6) and tumor necrosis factor (TNF). The development of hypoferraemia during inflammation requires hepcidin, an iron regulatory hormone, a disulfide-rich peptide, produced in the liver in response to the release of I1-6 during inflammation/infection. In conclusion, our results provide evidence that short-term administration of high doses of vitamin D determined diverse clinical signs and produced a marked increase of serum vitamin D3 and 25-OH-D3 and a marked alteration in the serum and tissue concentrations of Ca, Zn, Cu, and Fe. These changes depend on the doses given of vitamin D. PMID:22696892

Alarcón-Corredor, O M; Villarroel, J; Alfonso, R; Rondón, C

2011-09-01

214

Catalytic Chemistry.  

ERIC Educational Resources Information Center

Describes an approach for making chemistry relevant to everyday life. Involves the study of kinetics using the decomposition of hydrogen peroxide by vegetable juices. Allows students to design and carry out experiments and then draw conclusions from their results. (JRH)

Borer, Londa; And Others

1996-01-01

215

Chemistry Notes.  

ERIC Educational Resources Information Center

Describes equipment, activities, and experiments useful in chemistry instruction, including among others, a rapid method to determine available chlorine in bleach, simple flame testing apparatus, and a simple apparatus demonstrating the technique of flash photolysis. (SK)

School Science Review, 1980

1980-01-01

216

Catalytic Chemistry.  

ERIC Educational Resources Information Center

|Describes an approach for making chemistry relevant to everyday life. Involves the study of kinetics using the decomposition of hydrogen peroxide by vegetable juices. Allows students to design and carry out experiments and then draw conclusions from their results. (JRH)|

Borer, Londa; And Others

1996-01-01

217

Nuclear Chemistry.  

ERIC Educational Resources Information Center

Provides a brief review of the latest developments in nuclear chemistry. Nuclear research today is directed toward increased activity in radiopharmaceuticals and formation of new isotopes by high-energy, heavy-ion collisions. (Author/BB)

Chemical and Engineering News, 1979

1979-01-01

218

Green Chemistry  

NSDL National Science Digital Library

Learn about a study in which participants discovered contaminants in their homes, and how green chemistry may provide alternatives to such everyday toxins, in this video adapted from Contaminated Without Consent.

Foundation, Wgbh E.

2011-03-21

219

Chemistry Notes.  

ERIC Educational Resources Information Center

|Describes 13 activities, experiments and demonstrations, including the preparation of iron (III) chloride, simple alpha-helix model, investigating camping gas, redox reactions of some organic compounds, a liquid crystal thermometer, and the oxidation number concept in organic chemistry. (JN)|

School Science Review, 1981

1981-01-01

220

Nuclear Chemistry.  

ERIC Educational Resources Information Center

|Provides a brief review of the latest developments in nuclear chemistry. Nuclear research today is directed toward increased activity in radiopharmaceuticals and formation of new isotopes by high-energy, heavy-ion collisions. (Author/BB)|

Chemical and Engineering News, 1979

1979-01-01

221

Chemistry Notes  

ERIC Educational Resources Information Center

|Several ideas are proposed for chemistry teachers to try in their classrooms. Subjects included are polymerization of acrylate, polymerization of styrene, conductivity, pollution, preparation of chlorine, redox equations, chemiluminescence, and molecular sieves. (PS)|

School Science Review, 1973

1973-01-01

222

Chemistry Notes.  

ERIC Educational Resources Information Center

|Describes equipment, activities, and experiments useful in chemistry instruction, including among others, a rapid method to determine available chlorine in bleach, simple flame testing apparatus, and a simple apparatus demonstrating the technique of flash photolysis. (SK)|

School Science Review, 1980

1980-01-01

223

Organosilicon Chemistry.  

National Technical Information Service (NTIS)

Several new aspects of the chemistry of silylenes, divalent reactive silicon intermediates, have been explored. Insertion reactions of dimethylsilylene into O-H bonds of primary, secondary, and tertiary alcohols were studied. This provides an efficient ro...

W. P. Weber

1982-01-01

224

Variation in haematological parameters in children less than five years of age with asymptomatic Plasmodium infection: implication for malaria field studies.  

PubMed

During the season of high malaria transmission, most children are infected by Plasmodium, which targets red blood cells (RBCs), affecting haematological parameters. To describe these variations, we examined the haematological profiles of two groups of children living in a malaria-endemic area. A cross-sectional survey was conducted at the peak of the malaria transmission season in a rural area of Burkina Faso. After informed consent and clinical examination, blood samples were obtained from the participants for malaria diagnosis and a full blood count. Of the 414 children included in the analysis, 192 were not infected with Plasmodium, whereas 222 were asymptomatic carriers of Plasmodium infection. The mean age of the infected children was 41.8 months (range of 26.4-57.2) compared to 38.8 months (range of 22.4-55.2) for the control group (p = 0.06). The asymptomatic infected children tended to have a significantly lower mean haemoglobin level (10.8 g/dL vs. 10.4 g/dL; p < 0.001), mean lymphocyte count (4592/µL vs. 5141/µL; p = 0.004), mean platelet count (266 x 10³/µL vs. 385 x 10³/µL; p < 0.001) and mean RBC count (4.388 x 10(6)/µL vs. 4.158 x 10(6)/µL; p < 0.001) and a higher mean monocyte count (1403/µL vs. 1192/µL; p < 0.001) compared to the control group. Special attention should be applied when interpreting haematological parameters and evaluating immune responses in asymptomatic infected children living in malaria-endemic areas and enrolled in vaccine trials. PMID:23903982

Gansane, Adama; Ouedraogo, Issa Nebie; Henry, Noelie Bere; Soulama, Issiaka; Ouedraogo, Esperance; Yaro, Jean-Baptiste; Diarra, Amidou; Benjamin, Sombie; Konate, Amadou Tidiani; Tiono, Alfred; Sirima, Sodiomon Bienvenu

2013-08-01

225

Stratospheric chemistry  

SciTech Connect

Advances in stratospheric chemistry made by investigators in the United States from 1987 to 1990 are reviewed. Subject areas under consideration include photochemistry of the polar stratosphere, photochemistry of the global stratosphere, and assessments of inadvertent modification of the stratosphere by anthropogenic activity. Particular attention is given to early observations and theories, gas phase chemistry, Antarctic observations, Arctic observations, odd-oxygen, odd-hydrogen, odd-nitrogen, halogens, aerosols, modeling of stratospheric ozone, and reactive nitrogen effects.

Brune, W.H. (USAF, Geophysics Laboratory, Hanscom AFB, MA (United States))

1991-01-01

226

Chemistry Tutorials  

NSDL National Science Digital Library

The California State University Stanislaus developed these interactive chemistry Web tutorials to assist college students in mass spectrometry, proton NMR chemical shifts, and more. With the many animations and figures, visitors will find assistance with the subtraction and absorption of light and with infrared absorption frequencies for numerous compounds. The titration tutorials simulate laboratory experiments without the hazards of dealing with chemicals. Students will also find a very informative lesson describing how to use Excel to record and analyze their chemistry data.

227

Detection of invasive pulmonary aspergillosis in haematological malignancy patients by using lateral-flow technology.  

PubMed

Invasive pulmonary aspergillosis (IPA) is a leading cause of morbidity and mortality in haematological malignancy patients and hematopoietic stem cell transplant recipients(1). Detection of IPA represents a formidable diagnostic challenge and, in the absence of a 'gold standard', relies on a combination of clinical data and microbiology and histopathology where feasible. Diagnosis of IPA must conform to the European Organization for Research and Treatment of Cancer and the National Institute of Allergy and Infectious Diseases Mycology Study Group (EORTC/MSG) consensus defining "proven", "probable", and "possible" invasive fungal diseases(2). Currently, no nucleic acid-based tests have been externally validated for IPA detection and so polymerase chain reaction (PCR) is not included in current EORTC/MSG diagnostic criteria. Identification of Aspergillus in histological sections is problematic because of similarities in hyphal morphologies with other invasive fungal pathogens(3), and proven identification requires isolation of the etiologic agent in pure culture. Culture-based approaches rely on the availability of biopsy samples, but these are not always accessible in sick patients, and do not always yield viable propagules for culture when obtained. An important feature in the pathogenesis of Aspergillus is angio-invasion, a trait that provides opportunities to track the fungus immunologically using tests that detect characteristic antigenic signatures molecules in serum and bronchoalveolar lavage (BAL) fluids. This has led to the development of the Platelia enzyme immunoassay (GM-EIA) that detects Aspergillus galactomannan and a 'pan-fungal' assay (Fungitell test) that detects the conserved fungal cell wall component (1 ?3)-?-D-glucan, but not in the mucorales that lack this component in their cell walls(1,4). Issues surrounding the accuracy of these tests(1,4-6) has led to the recent development of next-generation monoclonal antibody (MAb)-based assays that detect surrogate markers of infection(1,5). Thornton(5) recently described the generation of an Aspergillus-specific MAb (JF5) using hybridoma technology and its use to develop an immuno-chromatographic lateral-flow device (LFD) for the point-of-care (POC) diagnosis of IPA. A major advantage of the LFD is its ability to detect activity since MAb JF5 binds to an extracellular glycoprotein antigen that is secreted during active growth of the fungus only(5). This is an important consideration when using fluids such as lung BAL for diagnosing IPA since Aspergillus spores are a common component of inhaled air. The utility of the device in diagnosing IPA has been demonstrated using an animal model of infection, where the LFD displayed improved sensitivity and specificity compared to the Platelia GM and Fungitell (1 ? 3)-?-D-glucan assays(7). Here, we present a simple LFD procedure to detect Aspergillus antigen in human serum and BAL fluids. Its speed and accuracy provides a novel adjunct point-of-care test for diagnosis of IPA in haematological malignancy patients. PMID:22473419

Thornton, Christopher; Johnson, Gemma; Agrawal, Samir

2012-03-22

228

Haematological and immunological responses to the tapeworm hymenolepis diminuta in man.  

PubMed

Self-infections with the tapeworm Hymenolepis diminuta were carried out to study the haematological and immunological responses in man. Infection caused pronounced eosinophilia, an increase in plasma viscosity and the production of parasite-specific IgG and IgM. Circulating IgE was not detected. There were no significant changes in erythrocyte values or serum transaminases. PMID:1162734

Turton, J A; Williamson, J R; Harris, W G

1975-06-01

229

Effect of storage time on haematological parameters in mullet, Mugil cephalus.  

PubMed

The aim of the present study was to assess the effect of storage time at +4?°C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0 ), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t-test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4?°C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One-way repeated-measures ANOVA showed a significant effect of storage time (P?haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4?°C because long-term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. PMID:23097308

Faggio, C; Casella, S; Arfuso, F; Marafioti, S; Piccione, G; Fazio, F

2012-10-24

230

Haematological factors associated with proliferative retinopathy in homozygous sickle cell disease  

Microsoft Academic Search

Haematological indices were investigated in 261 patients with homozygous sickle cell disease, 29 of whom had developed proliferative sickle retinopathy (PSR). After allowing for age-related effects, male patients with high Hb (> 9 g\\/dl) and low HbF (< 5%) levels appeared to constitute a high risk group for PSR. This relationship was not evident in females, PSR being observed in

R. J. Hayes; P. I. Condon; G. R. Serjeant

1981-01-01

231

HAEMATOLOGY AND SERUM BIOCHEMISTRY OF CAPTIVE UNSEDATED CHITAL DEER (AXIS AXIS') IN AUSTRALIA  

Microsoft Academic Search

Haematological and serum biochemical values were measured in blood samples col- lected over a 12-mo period from 37 unsedated chital deer (Axis axis). Stags and hinds, ranging in age from birth to maturity, were sampled. Haemoglobmn, total erythrocyte and haematocrit values were low at birth and sex differences were not apparent in neonates and juveniles, but were in adults. Chital

R. S. Chapple; A. W. English; R. C. MulIey; E. E. Lepherd

1991-01-01

232

Studies on an ascitic syndrome in young broilers 1. haematology and pathology  

Microsoft Academic Search

In the present investigation, the haematology and pathology of young broiler chicks with an ascitic syndrome were examined.Gross changes included cardiomegaly, ascites, congested lungs, shrunken livers, enlarged kidneys, dark breast muscle and congested intestines. Many blood parameters were significantly raised including the packed cell volume (PCV), haemoglobin (Hb), red (Rbc) and white blood cell (Wbc) counts. The heterophils and monocytes

M. H. Maxwell; G. W. Robertson; S. Spence

1986-01-01

233

Seasonal variation in haematological parameters in male and female Tinca tinca  

Microsoft Academic Search

This study was designed to investigate any seasonal (spring, summer, autumn and winter) changes in haematological parameters in the blood of Tinca tinca measuring the number of red blood cells (RBC), haematocrit, white blood cells, and total plasma proteins.The results show significant changes in RBC and haematocrit in males comparing spring and summer with autumn and winter, whereas in females

María Eugenia Collazos; Eduardo Ortega; Carmen Barriga; Ana Beatriz Rodrìguez

1998-01-01

234

Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats  

Microsoft Academic Search

ADEDAPO, A. A., M. O. ABATAN, O. O. OLORUNSOGO: Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats. Vet. arhiv 74, 53-62, 2004. ABSTRACT The toxic effects of 5 suspected poisonous plants of the genus Euphorbia (Euphorbia balsamifera Aiton, E. heterophylla L., E. hirta L., E. hyssopifolia L., and E. lateriflora Schum and

Adeolu A. Adedapo; Matthew O. Abatan; Olufunso O. Olorunsogo

2004-01-01

235

Effects of oral iron supplementation on some haematological parameters and iron biochemistry in neonatal dairy calves  

Microsoft Academic Search

Since the iron requirements of ruminants are not well established and most recommendations are estimates, this study was performed to determine whether 150mg\\/day iron supplementation in neonatal dairy calves (heifer replacement system) fed roughage and concentrates could promote a healthy haematology and iron profile. A total of 40 calves was used in the present study. The animals were divided into

M. Mohri; F. Sarrafzadeh; H. A. Seifi; N. Farzaneh

2004-01-01

236

Changes in haematological parameters and iron metabolism associated with a 1600 kilometre ultramarathon  

Microsoft Academic Search

OBJECTIVE: To investigate haematological variations and iron related changes in the serum of participants in a 1600 kilometre ultramarathon run. PARTICIPANTS: Seven male and two female participants in a 1600 km foot race. METHODS: Blood samples were obtained from the participants before, after four and 11 days of running, and at the end of the event. Samples were analysed by

K. E. Fallon; G. Sivyer; K. Sivyer; A. Dare

1999-01-01

237

Effect of acute exercise on some haematological parameters and neutrophil functions in active and inactive subjects  

Microsoft Academic Search

In this work we studied the possible effects of acute exercise on some haematological parameters and on some functions of neutrophils in seven active and six inactive subjects. Physical exercise (10 min on a cycle ergometer at a heart rate of 150 beats · min–1) induced a significant increase in total leucocyte, lymphocyte and neutrophil concentrations in active subjects; serum

G. Benoni; P. Bellavite; A. Adami; S. Chirumbolo; G. Lippi; G. Brocco; L. Cuzzolin

1995-01-01

238

Haematological changes in two thoroughbred horses in training with confirmed equine herpesvirus 1 infections  

Microsoft Academic Search

An outbreak of respiratory disease among thoroughbred horses in training in Hong Kong was caused by equine herpesvirus 1 (EHV-1) subtype 1 (abortion strain). Two of the horses affected by EHV-1 were serially blood sampled over a period of several weeks and their haematological values measured. There was an increase in monocyte count in the first few days which steadily

DK Mason; KL Watkins; CM Luk

1989-01-01

239

Oral health and quality of life among patients with head and neck cancer or haematological malignancies  

Microsoft Academic Search

The aim of the present study was to monitor the health-related quality of life (HRQOL) of patients with head and neck cancer or haematological malignancies during a period from before to after medical treatment, and to investigate the relationships between patient experiences of oral symptoms and HRQOL. The sample consisted of 41 consecutive patients. At the start, during and end

Kerstin E. O. Öhrn; Per-Olow Sjödén; Ylva-Britt Wahlin; Marie Elf

2001-01-01

240

Complementary and alternative medicine use in patients with haematological malignancies in Europe.  

PubMed

This study reports upon a descriptive cross-sectional survey assessing the use of complementary and alternative medicine (CAM) in patients with haematological cancers. Twelve European countries contributed data from patients with haematological cancers, as part of a larger study. Sixty-eight patients with haematological cancer participated. Among the participants, 26.5% used some form of CAM after the cancer diagnosis. The most common therapies used were homeopathy (38.9%), herbal medicine (22.2%) various psychic therapies, such as use of mediums, healers, rebirthing or past life regression therapy (22.2%). A particular profile of a CAM user was not evident in the sample. Moderate levels of satisfaction with CAM were reported. Patients commonly used CAM to increase the ability of their body to fight cancer and to improve physical and emotional well-being. Information about CAM was received mainly from friends or family. As CAM use in patients with haematological malignancies is common, clinicians should assist patients who want to use CAM to make an appropriate decision, and improve communication with them about CAM use in an open and non-judgemental dialogue. PMID:15955292

Molassiotis, Alexander; Margulies, Anita; Fernandez-Ortega, Paz; Pud, Dorit; Panteli, Vassiliki; Bruyns, Ingrid; Scott, Julia A; Gudmundsdottir, Gudbjorg; Browall, Maria; Madsen, Elin; Ozden, Gulten; Magri, Miriam; Selvekerova, Sarka; Platin, Nurgun; Kearney, Nora; Patiraki, Elisabeth

2005-05-01

241

Dielectric, haematological and biochemical studies of detergent toxicity in fish blood  

Microsoft Academic Search

Blood characteristics in dielectric, haematological and biochemical terms of the fish Cyprinus carpio exposed to a sublethal concentration of sodium alkyl benzene sulphonate were compared with those from untreated control fish; recovery from the test solution was also checked. Trends of change in the majority of chosen parameters of blood with time of fish exposure to anionic detergent were significantly

I. Bielinska

1987-01-01

242

Arbitrary primed PCR rules out Clostridium difficile cross-infection among patients in a haematology unit  

Microsoft Academic Search

Eight out of 20 (40%) patients with haematological malignancies hospitalized in the same unit of our hospital from 24 January to 24 April 1995, suffered from diarrhoea due to Clostridium difficile. The C. difficile isolates were characterized by serotyping and by arbitrary primed polymerase chain reaction (AP-PCR) using three different 10-mer oligonucleotides. It was found by serotyping that five patients

F. Lemann; C. Chambon; F. Barbut; C. Gardin; J. Brière; N. Lambert-Zechovsky; C. Branger

1997-01-01

243

Herpes simplex virus and intraoral ulcers in immunocompromised patients with haematologic malignancies  

Microsoft Academic Search

Possible factors predisposing to the development of intraoral ulcers in immunocompromised patients with haematologic malignancies were investigated. Among 46 patients undergoing antineoplastic treatment, 18 developed an intraoral ulcer during the prospective study. Patients with or without ulcers were comparable with respect to underlying disease, presence of teeth, the qualitative composition of the aerobic and facultatively anaerobic oral microflora, herpes simplex

O. J. Bergmann; S. C. Mogensen; J. Ellegaard

1990-01-01

244

The haematological, biochemical and immunological profile of athletes suffering from the overtraining syndrome  

Microsoft Academic Search

To help clarify the overtraining syndrome (OTS), a combination of parameters were measured in ten athletes who were suffering from OTS. Blood samples were obtained at rest and a range of haematological, biochemical and immunological tests were carried out on the samples. For each parameter, the mean value for the group was compared to an established normal range amongst age-matched

David G. Rowbottom; David Keast; Carmel Goodman; Alan R. Morton

1995-01-01

245

Paul Ehrlich (1854-1915): founder of chemotherapy and pioneer of haematology, immunology and oncology.  

PubMed

The Jewish-German histologist, microbiologist, bio- chemist, immunologist and pharmacologist Paul Ehrlich, by his research founded chemotherapy and pioneered im- munology, haematology and oncology. For his contribu- tion in immunology he was awarded the Nobel Prize of Medicine and Physiology in 1908. PMID:17415859

Androutsos, G

246

Recombinant erythropoietin in clinical practice  

PubMed Central

The introduction of recombinant human erythropoietin (RHuEPO) has revolutionised the treatment of patients with anaemia of chronic renal disease. Clinical studies have demonstrated that RHuEPO is also useful in various non-uraemic conditions including haematological and oncological disorders, prematurity, HIV infection, and perioperative therapies. Besides highlighting both the historical and functional aspects of RHuEPO, this review discusses the applications of RHuEPO in clinical practice and the potential problems of RHuEPO treatment.

Ng, T; Marx, G; Littlewood, T; Macdougall, I

2003-01-01

247

Clinical Chemistry and Clinical Toxicology Devices Panel ...  

Center for Biologics Evaluation and Research (CBER)

... for glucose test systems to address problems associated with using blood samples from alternate sites, such as the forearm, upper arm, thigh, calf ... More results from www.fda.gov/advisorycommittees/committeesmeetingmaterials/medicaldevices

248

Clinical Chemistry and Clinical Toxicology Devices Panel ...  

Center for Biologics Evaluation and Research (CBER)

... FDA asked for comment on whether the sponsor adequately addressed the issue of retention of drug in the hair from environmental exposure. ... More results from www.fda.gov/advisorycommittees/committeesmeetingmaterials/medicaldevices

249

Chemistry Now  

NSDL National Science Digital Library

The National Science Foundation (NSF) recently teamed up with NBC and the National Science Teachers Association to celebrate the International Year of Chemistry. Their big joint project was to create Chemistry Now, a weekly online video series that uncovers and explains the science of common, physical objects. There are over two dozen short films here that cover topics like the chemistry of salt, grapheme, safety glass, and the common cheeseburger. All of the videos are lively and interesting, and they can be used in a wide range of classroom settings to provide visual and audio reinforcement of topics that might be addressed in course lectures and other activities. The videos are completely free and the site includes links to other organizations that have created similar videos.

2012-08-17

250

Common Chemistry  

NSDL National Science Digital Library

A web resource that contains Chemical Abstracts Service (CAS) Registry Numbers for approximately 7,800 chemicals of widespread general public interest. Common Chemistry is helpful to non-chemists who know either a name or CAS Registry Number® of a common chemical and want to pair both pieces of information.

Chemical Abstracts Service (CAS)

251

Chemistry Notes  

ERIC Educational Resources Information Center

Short articles on the kinetics of the hydrogen peroxide-iodide ion reaction, simulation of fluidization catalysis, the use of Newman projection diagrams to represent steric relationships in organic chemistry, the use of synthetic substrates for proteolytic enzyme reactions, and two simple clock reactions"--hydrolysis of halogenoalkanes and…

School Science Review, 1972

1972-01-01

252

Chemistry Notes  

ERIC Educational Resources Information Center

|Short articles on the kinetics of the hydrogen peroxide-iodide ion reaction, simulation of fluidization catalysis, the use of Newman projection diagrams to represent steric relationships in organic chemistry, the use of synthetic substrates for proteolytic enzyme reactions, and two simple clock reactions"--hydrolysis of halogenoalkanes and…

School Science Review, 1972

1972-01-01

253

Chemistry Notes.  

ERIC Educational Resources Information Center

|Presents chemistry experiments, laboratory procedures, demonstrations, teaching suggestions, and classroom materials/activities. These include: game for teaching ionic formulas; method for balancing equations; description of useful redox series; computer programs (with listings) for water electrolysis simulation and for determining chemical…

School Science Review, 1983

1983-01-01

254

Chemistry Notes.  

ERIC Educational Resources Information Center

|Presents chemistry experiments, laboratory procedures, demonstrations, and classroom materials/activities. These include: experiments on colloids, processing of uranium ore, action of heat on carbonates; color test for phenols and aromatic amines; solvent properties of non-electrolytes; stereoscopic applications/methods; a valency balance;…

School Science Review, 1983

1983-01-01

255

Green Chemistry  

NSDL National Science Digital Library

This audio segment from PRI's The World Science Podcast explores the science of Green Chemistry. Hear about companies that are developing greener chemicals, and learn why they are fast becoming an attractive alternative for the multi-billion dollar chemical industry.

Foundation, Wgbh E.

2010-02-12

256

Chemistry Notes.  

ERIC Educational Resources Information Center

Presents procedures, experiments, demonstrations, teaching suggestions, and information on a variety of chemistry topics including, for example, inert gases, light-induced reactions, calculators, identification of substituted acetophenones, the elements, analysis of copper minerals, extraction of metallic strontium, equilibrium, halogens, and…

School Science Review, 1982

1982-01-01

257

Confectionary Chemistry.  

ERIC Educational Resources Information Center

|Presents activities and demonstrations that enable teachers to use various types of confections as tactile experiences to spark chemistry students' interest and generate enthusiasm for learning. Presents uses of candy in teaching about atomic structure, spontaneous nuclear decay, chemical formulas, fractoluminescence, the effect of a molecular…

Levine, Elise Hilf

1996-01-01

258

Chemistry Notes.  

ERIC Educational Resources Information Center

|Presents procedures, experiments, demonstrations, teaching suggestions, and information on a variety of chemistry topics including, for example, inert gases, light-induced reactions, calculators, identification of substituted acetophenones, the elements, analysis of copper minerals, extraction of metallic strontium, equilibrium, halogens, and…

School Science Review, 1982

1982-01-01

259

Chemistry's year  

NASA Astrophysics Data System (ADS)

The United Nations has proclaimed 2011 to be the International Year of Chemistry. Under this banner, chemists should seize the opportunity to highlight the rich history and successes of our subject to a much broader audience - and explain how it can help to solve the global challenges we face today and in the future.

2011-01-01

260

Chemistry Notes  

ERIC Educational Resources Information Center

|Describes several chemistry projects, including solubility, formula for magnesium oxide, dissociation of dinitrogen tetroxide, use of 1-chloro-2, 4-dinitrobenzene, migration of ions, heats of neutralizations, use of pocket calculators, sonic cleaning, oxidation states of manganese, and cell potentials. Includes an extract from Chemical Age on…

School Science Review, 1976

1976-01-01

261

Chlorine Chemistry  

NSDL National Science Digital Library

From poolcenter.com comes the Chlorine Chemistry Web site. Ten questions related to chlorine are answered such as What's the History of Chlorine, How Does Chlorine Work to Sanitize, and What Effect Does pH Have on Chlorine. Each is briefly explained in simple and non-technical language.

2008-01-25

262

Chemistry Activities  

NSDL National Science Digital Library

This collection of resource features demonstrations, laboratory investigations, teaching tips, worksheets and other chemistry-related activities. Materials include investigations of mols, nuclear energy, the periodic table, weight and mass, elements, calculations, equations, pH, atomic weight, half-lives, and reactions.

263

Effects of 2Phenoxyethanol Anaesthesia on Haematological Profi le on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss)  

Microsoft Academic Search

Velíšek J., Z. Svobodová, V. Pia?ková: Effects of 2-Phenoxyethanol Anaesthesia on Haematological Profi le on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss). Acta Vet. Brno 2007, 76: 487-492. The aim of this study was to assess changes in haematological profi le of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) after the exposure to anaesthetic 2-phenoxyethanol. The

Z. SVOBODOVÁ

2007-01-01

264

Factors influencing haematological recovery after allogeneic bone marrow transplantation in leukaemia patients treated with methotrexate-containing GVHD prophylaxis  

Microsoft Academic Search

In the present single institution study of 66 leukaemia patients (28 AML, 23 ALL, 15 CML), the factors influencing haematological\\u000a recovery after allogeneic bone marrow transplantation (alloBMT) were analysed retrospectively to identify the optimal conditions\\u000a required for rapid haematological recovery after alloBMT. All patients received GVHD prophylaxis with cyclosporine A plus\\u000a methotrexate. The mean number of days required to achieve

H. T. Hassan; C. Krog; M. Stockschläder; W. Zeller; W. Krüger; R. Erttmann; A. R. Zander

1997-01-01

265

Could interleukin-15 potentiate histone deacetylase inhibitor effects in haematological malignancy?  

PubMed

Despite significant progress in cancer therapy, prognosis in acute leukaemia remains dismal, and the development of new therapies is urgently warranted: in acute myeloid leukaemia, the current cure rate is of 30-40% in younger and much less in older patients. Chromatin remodeling through histone acetylation is one of the major mechanisms of transcriptional control of genes, and is involved in 'gene silencing' of antioncogenes in various tumour cells. Chromatin remodeling is also involved in transcriptional control of other genes, such as NKG2D ligand genes. Histone deacetylases and acetyltransferases are involved in the epigenetic regulation of gene expression, and increased/decreased activity of histone deacetylases has been reported in several cancer types. Histone deacetylase inhibitors were reportedly active in many cancers including hematological malignancies, and have been shown in numerous experiments to reduce cancer cell growth and enhance cell differentiation, growth arrest and apoptosis. In acute myeloid leukaemia, histone deacetylase inhibitors alone had limited efficacy, but their combination with other anticancer agents yielded promising results. Interleukin (IL)-15 is regarded with great hope in the immunotherapy of cancer, and IL-15-activated cytokine-induced killer cells showed potent antileukemic activity both in vitro and in vivo. IL-15 increases expression of NKG2D and its ligands and can increase natural killer cell mediated cytotoxicity against tumour cells. The administration of IL-15 was recently shown to be safe in preclinical models, and there are ongoing clinical trials of IL-15 in patients with cancer and HIV infection. We hypothesise that IL-15 will synergise with histone deacetylase inhibitors in increasing the levels of activatory NKG2D receptors on natural killer and CD8(+) T cells and of their ligands, the MHC class I related molecule A and B, on tumor cells, and will enhance innate immune antitumour responses in acute myeloid leukaemia and other haematological malignancies. Up-regulation of NKG2D-NKG2D-ligand antitumour immune response by combining histone deacetylase inhibitors with IL-15 has the potential to improve the efficacy of acute myeloid leukaemia treatment. PMID:23669372

Zdrenghea, Mihnea T

2013-05-10

266

Chemistry Gateways and Resources  

NSDL National Science Digital Library

The Chemistry Gateways and Resources collection is comprised of chemistry-related web portals, web sites, and individual digital resources pertaining to many areas of the discipline - general chemistry, organic and inorganic chemistry, physical chemistry, and others - and intended for a wide range of audiences: educators and learners, the general public, and chemistry research communities.

2008-03-14

267

Haematological and blood biochemical characteristics of Glyptosternum maculatum (Siluriformes: Sisoridae) in Xizang (Tibet).  

PubMed

This study investigated the haematological and blood biochemical characteristics of Glyptosternum maculatum. The haematological and biochemical parameters were measured in 30 adult fish collected from Nyingchi Reach of Yarlung Zangbo River in Tibet. The red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), erythrocyte osmotic fragility (maxEof and minEof), the erythrocyte sedimentation rate, mean cell volume (MCV), mean cellular haemoglobin content (MCH), and mean cell haemoglobin concentration (MCHC) were determined. Compared with other Siluriformes fishes, G. maculatum showed similar mean values for Hct, Hb, MCH, and MCHC and had slightly lower RBC and higher MCV. The biochemical parameters were assayed including alanine aminotransferase, aspartate aminotransferase (AST), alkaline phosphatase, total protein, albumin, globulin, albumin/globulin ratio, total bilirubin, direct bilirubin, urea, creatinine, glucose, total cholesterol, and triglyceride. The result showed that the value of AST in G. maculatum was obviously higher than that in Rhamdia quelen as well as in Silurus merdionalis. PMID:19757131

Zhang, Huijuan; Xie, Congxin; Li, Dapeng; Xiong, Dongmei; Liu, Haiping; Suolang, Sizhu; Shang, Peng

2009-09-10

268

Leukaemic transformation in patients with haematological disease receiving tumour necrosis factor inhibitors.  

PubMed

In recent years, there has been an increase in the use of tumour necrosis factor (TNF) inhibitors as treatment for several inflammatory conditions. However, the question of whether TNF inhibitors increase the risk of malignancies (including lymphoma and leukaemia) in these diseases remains controversial. Despite this concern, anti-TNF therapy is being used experimentally in the management of haematological patients with risk of leukaemic transformation such as myeloproliferative neoplasms. We report here the first ever reported case of blastic transformation in a patient with myelofibrosis under etanercept treatment for a severe hidradenitis suppurativa. Although etanercept provided a sustained partial response of the skin disease, the patient developed an acute myeloid leukaemia after 27 months on exclusively etanercept therapy. According to the Dynamic International Prognostic Scoring System-plus score, the patient had a low risk for leukaemic transformation. We discuss here the potential of TNF inhibitors to increase the already elevated risk of leukaemic transformation of these haematological diseases. PMID:22550963

Ferrer-Marín, Francisca; Amigo, Maria Luz; Vicente, Vicente

2012-06-01

269

Increased bone marrow microvascular density in haematological malignancies is associated with differential regulation of angiogenic factors  

Microsoft Academic Search

Antiangiogenic drugs are currently tested in haematological malignancies. As these drugs target different angiogenic regulators, and as cancers are inherently heterogeneous, a detailed characterization of angiogenesis in individual cancers is needed. Hence, we measured bone marrow microvessel density (MVD), plasma concentrations of eight angiogenesis-related parameters and the expression in blood mononuclear cells of 40 angiogenesis-related mRNAs in 93 patients with

H F S Negaard; N Iversen; I M Bowitz-Lothe; P M Sandset; B Steinsvik; B Østenstad; P O Iversen; HFS Negaard

2009-01-01

270

Haematological and spermatotoxic effects of ethylene glycol monomethyl ether in copper clad laminate factories  

PubMed Central

OBJECTIVES—To investigate the effects of ethylene glycol monomethyl ether (EGME) on haematology and reproduction in exposed workers.?METHODS—53 Impregnation workers from two factories that make copper clad laminate with EGME as a solvent were recruited as the exposed group. Another group of 121 lamination workers with indirect exposure to EGME was recruited as the control group. Environmental monitoring of concentrations of EGME in air and biological monitoring of urinary methoxyacetic acid (MAA) concentrations were performed. Venous blood was collected for routine and biochemical analyses. Semen was collected from 14 workers exposed to EGME for sperm analysis and was compared with 13 control workers.?RESULTS—Results of haematological examination showed that the haemoglobin, packed cell volume, and red blood cell count in the male workers exposed to EGME were significantly lower than in the controls. The frequency of anaemia in the exposed group (26.1%) was significantly higher than in the control group (3.2%). However, no differences were found between the female workers exposed and not exposed to EGME. After adjustment for sex, body mass index, and duration of employment, red blood cell count was significantly negatively associated with air concentrations of EGME, and haemoglobin, packed cell volume, and red blood cell count were significantly negatively associated with urinary concentrations of MAA. The pH of semen in the exposed workers was significantly lower than in the control workers, but there were no significant differences in the sperm count or sperm morphology between the exposed and control groups.?CONCLUSION—It can be concluded that EGME is a haematological toxin, which leads to anaemia in the exposed workers. However, the data from this study did not support the theory of a spermatotoxic effect of EGME.???Keywords: ethylene glycol monomethyl ether; haematological disorders; spermatotoxicity

Shih, T.; Hsieh, A.; Liao, G.; Chen, Y.; Liou, S.

2000-01-01

271

WELFARE AND HAEMATOLOGICAL INDICES OF WEANER RABBITS AS AFFECTED BY STOCKING DENSITY  

Microsoft Academic Search

Forty two weaner rabbits of mixed breeds and sexes with an average initial weight of 956.7±45.6 g were used to evaluate the effect of stocking densit y on welfare and haematological characteristics. Th e rabbits were allotted to four stocking densities of 10, 14.3, 20 and 25 rabbits\\/m 2. This corresponded to 2, 3, 4 and 5 rabbits per cage

272

Haematological and immunological response of calves to infection with Taenia saginata  

Microsoft Academic Search

Calves were infected with eggs ofTaenia saginata and the haematologic response and the antigen-induced responsiveness of their peripheral blood lymphocytes was followed. No changes were observed in the erythrocyte levels, but a leukocytosis developed. This was contributed to primarily by a lymphocytosis, but also by an eosinophilia. Antigen-induced lymphocyte blastogenesis measured the antigen-sensitive cell population in peripheral blood and this

S. Lloyd

1980-01-01

273

A data management software for the Sysmex NE 8000 haematology analyser  

Microsoft Academic Search

The Sysmex NE 8000 (TOA-Japan) is a haematology analyser that performs blood cells count and leukocyte differential count. For facilitating the work of technical validation, we developed a software adapted to any IBM® or compatible PC running under MS-DOS®, to manage the analyser. Data are automatically collected via the RS-232 interface from the analyser or keyed in for the other

J. P. Cambus; F. Nguyen; F. Cambus

1996-01-01

274

Effects of some plants of the spurge family on haematological and biochemical parameters in rats  

Microsoft Academic Search

AdEdApO, A. A., M. O. AbA t An, O. O. OlOrunsOgO: Effects of some plants of the spurge family on haematological and biochemical parameters in rats. Vet. arhiv 77, 29-38, 2007. AbstrAct The effects of five suspected poisonous plants of the spurge family ( euphorbiaceae ) i.e. alchornea cordifolia Schum and Thorn, Cnidoscolus acontifolius Mill, Phyllanthus amarus Schum and Thorn,

Adeolu Alex Adedapo; Matthew Oluwole Abatan; Olufunso Olabode Olorunsogo

275

Allogeneic transplant outcomes are not affected by body mass index (BMI) in patients with haematological malignancies  

Microsoft Academic Search

Bone marrow transplantation is frequently used as a consolidation therapy in patients with haematological malignancies to\\u000a improve the outcome of these patients. Obese individuals have larger absolute lean body and fat masses than non-obese individuals\\u000a of the same age, gender and height, which might lead to altered pharmacokinetics of chemotherapeutic agents. Data on the impact\\u000a of body mass on transplant

Emmanouil Nikolousis; Sandeep Nagra; Shankara Paneesha; Julio Delgado; Kathy Holder; Lynn Bratby; Sridhar Chaganti; Richard Lovell; Donald Milligan

2010-01-01

276

Erythropoietin acute reaction and haematological adaptations to short, intermittent hypobaric hypoxia  

Microsoft Academic Search

This study aimed to determine whether brief hypoxic stimuli in a hypobaric chamber are able to elicit erythropoietin (EPO)\\u000a secretion, and to effectively stimulate erythropoiesis in the short term. In two different experiments, a set of haematological,\\u000a biochemical, haemorheological, aerobic performance, and medical tests were performed in two groups of healthy subjects. In\\u000a the first experiment, the mean plasma concentration

Ferran A. Rodríguez; Josep L. Ventura; Mireia Casas; Héctor Casas; Teresa Pagés; Ramón Rama; Antoni Ricart; Luis Palacios; Ginés Viscor

2000-01-01

277

Haematological analysis conducted at the departure of the Tour de France 2001.  

PubMed

Since the introduction of blood analysis performed before major cycling events in 1997, there have been discussions concerning the quality of the results. The aim of our study therefore was to measure blood samples and compare the results obtained on the field with those obtained in the laboratory. For this it was necessary to have blood samples analysed with different instruments to determine the exactness of the results and evaluate the performances of these instruments in order to validate the haematological testing used to reveal athletes abusing recombinant erythropoietin. We report on the haematological analysis of 177 professional cyclists who took part in the Tour de France 2001. All the blood samples were withdrawn in the morning between 7 and 9 am in Dunkerque (France) and were analysed immediately with a transportable analyser. Then the samples were flown to Lausanne (Switzerland) and were reanalysed in two independent ISO 17 025 accredited laboratories with three different analysers. The results confirmed that the most effective haematological follow-up should be performed under standardized pre-analytical conditions and with identical analysers of the same manufacturer to avoid too many variations notably on the haematocrit level and the reticulocyte count. Furthermore, this study suggests that analyses performed on the site are good and could enable the federations to perform a urinary test to detect rhEPO abuse right after the blood analysis. This time saving is essential to fight efficiently recombinant erythropoietin doping, because the half life of the hormone is very short. PMID:15776340

Robinson, N; Schattenberg, L; Zorzoli, M; Mangin, P; Saugy, M

2005-04-01

278

The efficacy of nardostachys jatamansi against the radiation induced haematological damage in rats.  

PubMed

Introduction: Radiation is increasingly being used for medical purposes and it is an established weapon in the diagnosis and the therapy of cancer. An exposure to 1-2 Gys causes the NVD (Nausea, vomiting, diarrhoea) syndrome, whereas an exposure to 2-6 Gys causes the haematopoietic syndrome. The aim of the present study was to investigate the protective effect of the Nardostachys jatamansi root extract (NJE) on the radiation induced haematological damage in rats. Materials and Methods: EBR was performed at the Microtron Centre, Mangalore University, India. Rats were treated with NJE once daily for 15 days before and after the irradiation. After the irradiation, blood was collected for determining the peripheral blood counts (RBC and WBC), haemoglobin, the platelet count and the packed cell volume (PCV) at 6 hours, 12 hours, 24 hours, 48 hours and 5, 10 and 15 days post irradiation. The data was analyzed by one way ANOVA, followed by the Tukey's test for multiple comparisons. Result: NJE provided protection against the radiation induced haematological disorders. The rats treated with NJE exhibited a time dependent significant elevation in all the haematological parameters which were studied and its modulation upto the near normal level was recorded. Conclusion: From this study, we concluded that, NJE provides protection by modulating the radiation induced damage on the haematopoietic system. PMID:23905085

Gowda, Damodara K M; Shetty, Lathika; A P, Krishna; Kumari, Suchetha N; Sanjeev, Ganesh; P, Naveen

2013-04-26

279

Immune reconstitution in children following chemotherapy for haematological malignancies: a long-term follow-up.  

PubMed

Modern intensive chemotherapy for childhood haematological malignancies has led to high cure rates, but has detrimental effects on the immune system. There is little knowledge concerning long-term recovery of the adaptive immune system. Here we studied the long-term reconstitution of the adaptive immune system in 31 children treated for haematological malignancies between July 2000 and October 2006. We performed detailed phenotypical and functional analyses of the various B and T cell subpopulations until 5 years after chemotherapy. We show that recovery of newly-developed transitional B cells and naive B and T cells occurred rapidly, within months, whereas recovery of the different memory B and T cell subpopulations was slower and incomplete, even after 5 years post-chemotherapy. The speed of B and T cell recovery was age-independent, despite a significant contribution of the thymus to T cell recovery. Plasmablast B cell levels remained above normal and immunoglobulin levels normalised within 1 week. Functional T cell responses were normal, even within the first year post-chemotherapy. This study shows that after intensive chemotherapy for haematological malignancies in children, numbers of several memory B and T cell subpopulations were decreased on the long term, while functional T cell responses were not compromised. PMID:21114483

van Tilburg, Cornelis M; van Gent, Rogier; Bierings, Marc B; Otto, Sigrid A; Sanders, Elisabeth A M; Nibbelke, Elisabeth E; Gaiser, Jacobus F; Janssens-Korpela, Pirkko L; Wolfs, Tom F W; Bloem, Andries C; Borghans, José A M; Tesselaar, Kiki

2010-11-28

280

Alcohol drinking, tobacco smoking and subtypes of haematological malignancy in the UK Million Women Study  

PubMed Central

Background: Previous research suggests associations of lower alcohol intake and higher tobacco consumption with increased risks of haematological malignancy. The prospective Million Women Study provides sufficient power for reliable estimates of subtype-specific associations in women. Methods: Approximately 1.3 million middle-aged women were recruited in the United Kingdom during 1996–2001 and followed for death, emigration and cancer registration until 2009 (mean 10.3 years per woman); potential risk factors were assessed by questionnaire. Adjusted relative risks were estimated by Cox regression. Results: During follow-up, 9162 incident cases of haematological malignancy were recorded, including 7047 lymphoid and 2072 myeloid cancers. Among predominantly moderate alcohol drinkers, higher intake was associated with lower risk of lymphoid malignancies, in particular diffuse large B-cell lymphoma (relative risk 0.85 per 10?g alcohol per day (95% confidence interval 0.75–0.96)), follicular lymphoma (0.86 (0.76–0.98)) and plasma cell neoplasms (0.86 (0.77–0.96)). Among never- and current smokers, higher cigarette consumption was associated with increased risk of Hodgkin lymphoma (1.45 per 10 cigarettes per day (1.22–1.72)), mature T-cell malignancies (1.38 (1.10–1.73)) and myeloproliferative/myelodysplastic disease (1.42 (1.31–1.55)). Conclusion: These findings confirm and extend existing evidence for associations of subtypes of haematological malignancy with two common exposures in women.

Kroll, M E; Murphy, F; Pirie, K; Reeves, G K; Green, J; Beral, V

2012-01-01

281

Haematological and biochemical toxicity induced by methanol in rats: ameliorative effects of Opuntia vulgaris fruit extract.  

PubMed

The ameliorative effects of Opuntia vulgaris fruit extract (OE) were evaluated against methanol-induced haematological and biochemical toxicity in rats. The methanol-induced haematological and biochemical perturbation significantly decreased the levels of red blood cell (RBC), haemoglobin (Hb), haematocrit (Ht), serum total protein and increased glucose, cholesterol, and triglyceride levels in serum. Treatment of rats with methanol significantly increased lipid peroxidation (LPO) level and decreased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in erythrocytes. OE treatment could increase significantly the levels of RBC, Hb, Ht and total protein, and decrease glucose, cholesterol and triglyceride levels in serum, and increase the activities of SOD, CAT and GPx in erythrocytes, when compared with methanol-treated group. Spleen histopathology showed that OE could significantly reduce the incidence of spleen lesion induced by methanol. These results suggested that OE could exhibit a potential source of natural antioxidants against methanol-induced haematological and biochemical disruption in rats. The protective effects of OE may be due to the modulation of antioxidant enzymes activities and inhibition of LPO. PMID:21422078

Saoudi, Mongi; Jebahi, Samira; Jamoussi, Kamel; Ben Salah, Ghada; Kallel, Choumous; El Feki, Abdelfattah

2011-03-21

282

The influence of administering "effective microorganisms" to pullets on chosen haematological and biochemical blood indexes.  

PubMed

"Effective Microorganisms" (EM)--a mixture of lactic acid bacteria, photosynthetic bacteria, yeasts and fungi are used mainly in agriculture and organic waste treatment. Recently, they have also been added to water and feed for animals, as well as to processing their excrements into compost and to eliminate the stench. The objective of the present study was to assess the influence of a 14-day administration of an EM solution in drinking water to layer hens on chosen haematological and biochemical indexes. The research was carried out on 120 hens divided into two equal groups. The birds in the experimental group were given drinking water with dissolved EM (5% solution), and those in the control group--water without the preparation. On the 64th day of the aviculture, the hens were weighted and their blood was taken from the wing vein for haematological and biochemical examinations. Administering EM with water to hens did not influence significantly their body weight nor chosen haematological and biochemical indexes. A significant increase was found only in the number of platelets, the level of albumins, the content of total cholesterol and the LDH activity, however, a decrease in the ALT activity was observed. PMID:20169927

Sokó?, R; Michalczyk, M; Spodniewska, A; Barski, D

2009-01-01

283

Incidence of haematological malignancies by ethnic group in England, 2001-7.  

PubMed

The aetiology of most haematological malignancies is largely unknown. Studies of migrant populations can provide insights into the relative importance of genetic and environmental risk factors for these diseases. This study compares incidence rates in British Indians, Pakistanis, Bangladeshis, Black Africans, Black Caribbeans, Chinese and Whites in England from 2001 to 2007. We analysed 134 302 haematological cancer registrations with ethnicity obtained by linkage to the Hospital Episodes Statistics database. Mid-year population estimates from 2001 to 2007 were used. Incidence rate ratios adjusted for age, sex and income were calculated, comparing the six ethnic groups to Whites and to each other. Whites had the highest rates for most subtypes. However, Blacks experienced more than double the incidence of plasma cell and mature T-cell neoplasms compared to other ethnic groups. There were also significant differences in incidence between Indians, Pakistanis and Bangladeshis for Hodgkin lymphoma and mature B-cell neoplasms and between Black African and Black Caribbeans for mature B-cell and other lymphoid neoplasms (all P < 0·001). Our results show that the risk of haematological cancers varies greatly by ethnic group, including within those groups that have traditionally been grouped together (South Asians and Blacks) with many of these differences not explicable by known risk factors. PMID:24033296

Shirley, Megan H; Sayeed, Shameq; Barnes, Isobel; Finlayson, Alexander; Ali, Raghib

2013-09-14

284

Evaluation of Computerized Medicinal Chemistry Case Study Modules as Tools to Enhance Student Learning and Clinical Problem-Solving Skills1  

Microsoft Academic Search

This national controlled study assesses the impact of computerized case studies that emphasize medici- nal chemistry content on pharmacy students' ability to learn and apply chemical information and principles to solve complex therapeutic problems. Eighty-six student volunteers from five schools or colleges of phar- macy were assigned to Control and Experimental groups. There was no significant difference between these study

Victoria F. Roche; Marlene J. Aitken

1999-01-01

285

Epidemiology and sites of involvement of invasive fungal infections in patients with haematological malignancies: a 20-year autopsy study.  

PubMed

Autopsy studies remain an essential tool for understanding the patterns of fungal disease not detected ante mortem with current diagnostic approaches. We collected data concerning the microbiological trends, patient clinical characteristics and sites of involvement for invasive fungal infections (IFIs) identified at autopsy in a single large cancer treatment centre over a 20-year period (1989-2008). The autopsy rate and IFI prevalence both declined significantly during the study period. The prevalence of Aspergillus spp. decreased significantly from the first 15 years of the study (from 0.12 to 0.14 cases per 100 autopsies to 0.07 in 2004-2008; P = 0.04), with only Mucorales accounting for a greater proportion of IFIs over the duration of the study period (0.06 to 0.2 cases per 100 autopsies, P = 0.04). After 2003, moulds accounted for the majority of infections identified at autopsy in the spleen, kidney, heart and gastrointestinal tract. Despite a trend of decreasing prevalence from 1989 to 2004, invasive candidiasis increased in prevalence during later periods 2004-2008 (0.02-0.05 per 100 autopsies) with decreasing kidney, heart and spleen involvement. Despite a declining autopsy rate, these data suggest a decreasing prevalence overall of IFIs with changing patterns of dissemination in patients with haematological malignancies. PMID:23551865

Lewis, Russell E; Cahyame-Zuniga, Lizebeth; Leventakos, Konstantinos; Chamilos, Georgios; Ben-Ami, Ronen; Tamboli, Pheroze; Tarrand, Jeffrey; Bodey, Gerald P; Luna, Mario; Kontoyiannis, Dimitrios P

2013-04-04

286

Resin Chemistry  

Microsoft Academic Search

\\u000a During the ensuing years since the last phenolic resins book was published, many new and remarkable developments have occurred\\u000a in the realm of phenolic chemistry and are given in this chapter.\\u000a \\u000a \\u000a A critical examination of the first step or addition step (methylolation) in the preparation of resoles is described and how\\u000a it can be controlled and compared with the typical

Louis Pilato

2010-01-01

287

Stability of haematological parameters and its relevance on the athlete's biological passport model.  

PubMed

The stability of haematological parameters is crucial to guarantee accurate and reliable data for implementing and interpreting the athlete's biological passport (ABP). In this model, the values of haemoglobin, reticulocytes and out-of-doping period (OFF)-score (Hb-60?Ret) are used to monitor the possible variations of those parameters, and also to compare the thresholds developed by the statistical model for the single athlete on the basis of its personal values and the variance of parameters in the modal group. Nevertheless, a critical review of the current scientific literature dealing with the stability of the haematological parameters included in the ABP programme, and which are used for evaluating the probability of anomalies in the athlete's profile, is currently lacking. In addition, we collected information from published studies, in order to supply a useful, practical and updated review to sports physicians and haematologists. There are some parameters that are highly stable, such as haemoglobin and erythrocytes (red blood cells [RBCs]), whereas others, (e.g. reticulocytes, mean RBC volume and haematocrit) appear less stable. Regardless of the methodology, the stability of haematological parameters is improved by sample refrigeration. The stability of all parameters is highly affected from high storage temperatures, whereas the stability of RBCs and haematocrit is affected by initial freezing followed by refrigeration. Transport and rotation of tubes do not substantially influence any haematological parameter except for reticulocytes. In all the studies we reviewed that used Sysmex instrumentation, which is recommended for ABP measurements, stability was shown for 72 hours at 4 ° C for haemoglobin, RBCs and mean curpuscular haemoglobin concentration (MCHC); up to 48 hours for reticulocytes; and up to 24 hours for haematocrit. In one study, Sysmex instrumentation shows stability extended up to 72 hours at 4 ° C for all the parameters. There are significant differences among methods and instruments: Siemens Advia shows lower stability than Sysmex as regards to reticulocytes. However, the limit of 36 hours from blood collection to analysis as recommended by ABP scientists is reasonable to guarantee analytical quality, when samples are transported at 4 ° C and are accompanied by a certified steadiness of this temperature. There are some parameters that are highly stable, such as haemoglobin and RBCs; whereas others, such as reticulocytes, mean cell volume and haematocrit are more unstable. The stability of haematological parameters might be improved independently from the analytical methodology, by refrigeration of the specimens. PMID:22060177

Lombardi, Giovanni; Lanteri, Patrizia; Colombini, Alessandra; Lippi, Giuseppe; Banfi, Giuseppe

2011-12-01

288

Medicinal chemistry for 2020  

PubMed Central

Rapid advances in our collective understanding of biomolecular structure and, in concert, of biochemical systems, coupled with developments in computational methods, have massively impacted the field of medicinal chemistry over the past two decades, with even greater changes appearing on the horizon. In this perspective, we endeavor to profile some of the most prominent determinants of change and speculate as to further evolution that may consequently occur during the next decade. The five main angles to be addressed are: protein–protein interactions; peptides and peptidomimetics; molecular diversity and pharmacological space; molecular pharmacodynamics (significance, potential and challenges); and early-stage clinical efficacy and safety. We then consider, in light of these, the future of medicinal chemistry and the educational preparation that will be required for future medicinal chemists.

Satyanarayanajois, Seetharama D; Hill, Ronald A

2011-01-01

289

High-dose carboplatin, etoposide and melphalan (CEM) with peripheral blood progenitor cell support as late intensification for high-risk cancer: non-haematological, haematological toxicities and role of growth factor administration.  

PubMed Central

The present report describes the non-haematological toxicity and the influence of growth factor administration on haematological toxicity and haematopoietic recovery observed after high-dose carboplatin (1200 mg m(-2)), etoposide (900 mg m(-2)) and melphalan (100 mg m(-2)) (CEM) followed by peripheral blood progenitor cell transplantation (PBPCT) in 40 patients with high-risk cancer during their first-line treatment. PBPCs were collected during the previous outpatient induction chemotherapy programme by leukaphereses. CEM administration with PBPCT was associated with low non-haematological toxicity and the only significant toxicity consisted of a reversible grade III/IV increase in liver enzymes in 32% of the patients. Haematopoietic recovery was very fast in all patients and the administration of granulocyte colony-stimulating factor (G-CSF) plus erythropoietin (EPO) or granulocyte-macrophage colony-stimulating factor (GM-CSF) plus EPO after PBPCT significantly reduced haematological toxicity, abrogated antibiotic administration during neutropenia and significantly reduced hospital stay and patient's hospital charge compared with patients treated with PBPCT only. None of the patients died early of CEM plus PBPCT-related complications. Low non-haematological toxicity and accelerated haematopoietic recovery renders CEM with PBPC/growth factor support an acceptable therapeutic approach in an adjuvant or neoadjuvant setting.

Benedetti Panici, P.; Pierelli, L.; Scambia, G.; Foddai, M. L.; Salerno, M. G.; Menichella, G.; Vittori, M.; Maneschi, F.; Caracussi, U.; Serafini, R.; Leone, G.; Mancuso, S.

1997-01-01

290

Looking Forward in Pharmaceutical Process Chemistry  

Microsoft Academic Search

Core activities of process chemistry involve the synthesis of drug candidates at a scale that supports clinical evaluations and the creation of a manufacturing process that is safe, robust, and cost effective. Innovative problem solving has always been a key element in process chemistry and will be increasingly important for addressing emerging challenges in science, globalization, and commoditization. The acquisition

Ian W. Davies; Christopher J. Welch

2009-01-01

291

(Pesticide chemistry)  

SciTech Connect

This report summarizes a trip by L. W. Barnthouse of the Environmental Sciences Division (ESD), Oak Ridge National Laboratory (ORNL), to Hamburg, Federal Republic of Germany (FRG), where he participated in the 7th International Congress of Pesticide Chemistry. He chaired a workshop on experimental systems for determining effects of pesticides on nontarget organisms and gave an oral presentation at a symposium on pesticide risk assessment. Before returning to the United States, Dr. Barnthouse visited the Netherlands Institute for Sea Research in Texel, the Netherlands.

Barnthouse, L.W.

1990-09-04

292

Progress in fighting systemic fungal infections in haematological neoplasia.  

PubMed

Considering the limited data available, there is clearly a need for thorough, well-designed clinical research on the epidemiology, diagnosis, treatment and prevention of invasive fungal infection in patients who are treated for cancer. Our knowledge has increased, but the information obtained so far is patchy and not generally applicable, as it is influenced by local problems and circumstances. New diagnostic tools have become available, but they are still insufficient in many cases. Until the value of the presently available chemoprophylaxis has been established beyond doubt, the strategy should be one of wait-and-see for patients with a low or moderate risk of developing infection. In bone marrow transplant recipients fluconazole has shown favourable results in eliminating yeast infections, but in patients at high risk of mould infections early initiation of intravenous treatment with amphotericin B at a therapeutic dose remains the best approach. The question of the optimal time point to start empirical antifungal treatment remains and has even been extended by the dispute about what antifungal drugs should be used for this purpose. Amphotericin B is still the drug of choice for the treatment of disseminated fungal infection, but its lipid formulations seem to offer a safer, though far more expensive, alternative. Head-to-head comparisons between the different formulations are required before a final conclusion on their respective efficacies and toxicities can be drawn, and it is questionable whether a higher dose will produce better results. Fluconazole appears very useful against the majority of Candida infections, whereas itraconazole is effective against both yeast and moulds, providing that adequate resorption can be ensured. The results of the first clinical trial of voriconazole in pulmonary aspergillosis have proved very promising. PMID:9458534

De Pauw, B E; Meis, J F

1998-01-01

293

Industrial Chemistry and School Chemistry: Making chemistry studies more relevant  

Microsoft Academic Search

In this paper, we present the development and implementation over the period of more than 15 years of learning materials focusing on industrial chemistry as the main theme. The work was conducted in the Department of Science Teaching at the Weizmann Institute of Science, Israel. The project's general goal was to teach chemistry concepts in the context of industrial chemistry

Avi Hofstein; Miri Kesner

2006-01-01

294

Environmental chemistry. Seventh edition  

SciTech Connect

This book presents a basic understanding of environmental chemistry and its applications. In addition to providing updated materials in this field, the book emphasizes the major concepts essential to the practice of environmental chemistry. Topics of discussion include the following: toxicological chemistry; toxicological chemistry of chemical substances; chemical analysis of water and wastewater; chemical analysis of wastes and solids; air and gas analysis; chemical analysis of biological materials and xenobiotics; fundamentals of chemistry; and fundamentals of organic chemistry.

Manahan, S.E. [Univ. of Missouri, Columbia, MO (United States)

1999-11-01

295

Evaluation of sulfonamide detoxification pathways in haematologic malignancy patients prior to intermittent trimethoprim-sulfamethoxazole prophylaxis  

PubMed Central

AIMS Patients with haematologic malignancies have a reportedly high incidence of sulfamethoxazole (SMX) hypersensitivity. The objective of this study was to determine whether deficiencies in sulfonamide detoxification pathways, to include glutathione (GSH) and ascorbate (AA), and cytochrome b5 (b5) and cytochrome b5 reductase (b5R), were prevalent in these patients. A secondary pilot objective was to determine whether the incidence of drug hypersensitivity following intermittent trimethoprim-SMX (TMP-SMX) prophylaxis approached that reported for high dose daily regimens. METHODS Forty adult patients with haematologic malignancies (HM) and 35 healthy adults were studied; an additional 13 HM patients taking ascorbate supplements (HM-AA) were also evaluated. Twenty-two of 40 HM patients were prescribed and were compliant with TMP-SMX 960 mg three to four times weekly. RESULTS There were no significant differences between HM and healthy groups in plasma AA (median 37.2 µmvs. 33.9 µm) or red blood cell GSH (1.9 mmvs. 1.8 mm). However, plasma AA was correlated significantly with leucocyte b5/b5R reduction (r = 0.39, P = 0.002). Deficient b5/b5R activities were not found in HM patients. In fact, patients with chronic lymphocytic leukaemia or myeloma had significantly higher median activities (80.7 µmol mg–1 min?1) than controls (18.9 µmol mg–1 min?1, P = 0.008). After 3–4 weeks of treatment, no patients developed SMX-specific T cells and only one patient developed rash. CONCLUSIONS Deficiencies of blood antioxidants and b5/b5R reduction were not found in this population with haematologic malignancies, and the development of skin rash and drug-specific T cells appeared to be uncommon with intermittent TMP-SMX prophylaxis.

Abouraya, Mahmoud; Sacco, James C; Kahl, Brad S; Trepanier, Lauren A

2011-01-01

296

Focus on invasive mucormycosis in paediatric haematology oncology patients: a series of 11 cases.  

PubMed

Mucormycosis has emerged as an increasingly important infection in oncology centres with high mortality, especially in severely immunocompromised patients. We carried out a retrospective study of 11 children with mucormycosis treated in seven French oncology-haematology paediatric wards during the period from 1991 to 2011. Lichtheimia corymbifera and Mucor spp. were the predominant pathogens. Treatment regimens included antifungal therapy, reversal of underlying predisposing risk factors and surgical debridement. Although mucormycosis is associated with high mortality, this infection could be cured in eight of our cases of severely immunocompromised paediatric cancer patients. PMID:23020159

Phulpin-Weibel, Aurélie; Rivier, Alexandre; Leblanc, Thierry; Bertrand, Yves; Chastagner, Pascal

2012-10-01

297

New aspects of the insulin resistance syndrome: impact on haematological parameters  

Microsoft Academic Search

.\\u000a Aim\\/hypothesis:   Previous studies have shown that insulin has an important in vitro role in the regulation of human erythropoiesis. We investigated\\u000a whether in vivo hyperinsulinaemia\\/insulin resistance affects haematological parameters. \\u000a \\u000a \\u000a \\u000a Methods:   A total of 608 subjects between 22 and 99 years of age were enrolled in the Chianti study, an epidemiological study of factors\\u000a affecting mobility in old age. The

M. Barbieri; E. Ragno; E. Benvenuti; G. A. Zito; A. Corsi; L. Ferrucci; G. Paolisso

2001-01-01

298

Disseminated mucormycosis in haematological patients: CT and MRI findings with pathological correlation.  

PubMed

Disseminated mucormycosis is a rare, mostly fatal infectious complication in immunocompromised haematological patients. The purpose of our study was to describe the multiorgan manifestations of disseminated mucormycosis documented at CT and MRI in four patients and correlate these with the pathological findings and patient outcome. Irrespective of the site of infection, infarction or haemorrhage are the constant features of invasive mycosis. Identification of one or both of these two major imaging findings in immunocompromised patients should be regarded as an indicator of possible infection by angiotropic fungi, including the genre Mucorales. PMID:16940368

Horger, M; Hebart, H; Schimmel, H; Vogel, M; Brodoefel, H; Oechsle, K; Hahn, U; Mittelbronn, M; Bethge, W; Claussen, C D

2006-09-01

299

What is Chemistry?  

NSDL National Science Digital Library

Provided by Kiwi Web Chemistry & New Zealand, the "What is Chemistry?" Web site offers a wealth of chemistry information and links. Visitors can read the definition of and link to other sites on inorganic chemistry, organic chemistry, analytical chemistry, physical chemistry, stoichiometry, nuclear chemistry, alchemy, and biochemistry. Other pages of the site explore acids and bases, redox reductions, equations, moles, periodic tables, and more. This extensive and interesting site does a good job of clearly explaining some difficult concepts as well as providing educators and students a good source of other quality sites containing similar content.

Campbell, Allan

300

Astronomical chemistry.  

PubMed

The discovery of polar polyatomic molecules in higher-density regions of the interstellar medium by means of their rotational emission detected by radioastronomy has changed our conception of the universe from essentially atomic to highly molecular. We discuss models for molecule formation, emphasizing the general lack of thermodynamic equilibrium. Detailed chemical kinetics is needed to understand molecule formation as well as destruction. Ion molecule reactions appear to be an important class for the generally low temperatures of the interstellar medium. The need for the intrinsically high-quality factor of rotational transitions to definitively pin down molecular emitters has been well established by radioastronomy. The observation of abundant molecular ions both positive and, as recently observed, negative provides benchmarks for chemical kinetic schemes. Of considerable importance in guiding our understanding of astronomical chemistry is the fact that the larger molecules (with more than five atoms) are all organic. PMID:21128763

Klemperer, William

2011-01-01

301

Astronomical Chemistry  

NASA Astrophysics Data System (ADS)

The discovery of polar polyatomic molecules in higher-density regions of the interstellar medium by means of their rotational emission detected by radioastronomy has changed our conception of the universe from essentially atomic to highly molecular. We discuss models for molecule formation, emphasizing the general lack of thermodynamic equilibrium. Detailed chemical kinetics is needed to understand molecule formation as well as destruction. Ion molecule reactions appear to be an important class for the generally low temperatures of the interstellar medium. The need for the intrinsically high-quality factor of rotational transitions to definitively pin down molecular emitters has been well established by radioastronomy. The observation of abundant molecular ions both positive and, as recently observed, negative provides benchmarks for chemical kinetic schemes. Of considerable importance in guiding our understanding of astronomical chemistry is the fact that the larger molecules (with more than five atoms) are all organic.

Klemperer, William

2011-05-01

302

Radiation Research: Chemistry and Physics.  

National Technical Information Service (NTIS)

Papers in this Proceedings are grouped under the following headings: (1) primary processes in radiation physics and chemistry; (2) radiation chemistry - general chemistry; (3) radiation chemistry - DNA and model systems; (4) radiation chemistry - other mo...

J. J. Broerse G. W. Barendsen H. B. Kal A. J. van der Kogel

1983-01-01

303

Physico-chemical properties of a novel (-)-hydroxycitric acid extract and its effect on body weight, selected organ weights, hepatic lipid peroxidation and DNA fragmentation, hematology and clinical chemistry, and histopathological changes over a period of 90 days.  

PubMed

Garcinia cambogia-derived (-)-hydroxycitric acid (HCA) is a popular and natural supplement for weight management. HCA is a competitive inhibitor of the enzyme ATP citrate lyase, which catalyzes the conversion of citrate and coenzyme A to oxaloacetate and acetyl coenzyme A (acetyl CoA) in the cytosol. Acetyl CoA is used in the synthesis of fatty acids, cholesterol and triglycerides, and in the synthesis of acetylcholine in the central nervous system. Studies have demonstrated the efficacy of a novel 60% calcium-potassium salt of HCA derived from Garcinia cambogia (HCA-SX, Super CitriMax) in weight management. Results have shown that HCA-SX promotes fat oxidation, enhances serotonin release and availability in the brain cortex, normalizes lipid profiles, and lowers serum leptin levels in obese subjects. Acute oral, acute dermal, primary dermal irritation and primary eye irritation toxicity, as well as Ames bacterial reverse mutation studies and mouse lymphoma tests have demonstrated the safety of HCA-SX. However, no detailed long-term safety of HCA-SX or any other HCA extract has been previously assessed. We evaluated the dose- and time-dependent effects of HCA-SX in Sprague-Dawley rats on body weight, selected organ weights, hepatic lipid peroxidation and DNA fragmentation, hematology and clinical chemistry over a period of 90 days. Furthermore, a 90-day histopathological evaluation was conducted. The animals were treated with 0, 0.2, 2.0 and 5.0% HCA-SX of feed intake and were sacrificed on 30, 60 or 90 days of treatment. The body weight and selected organ weights were assessed and correlated as a % of body weight and brain weight at 90 days of treatment. A significant reduction in body weight was observed in treated rats as compared to control animals. An advancing age-induced marginal increase in hepatic lipid peroxidation was observed in both male and female rats, while no such difference in hepatic DNA fragmentation was observed as compared to the control animals. Furthermore, selected organ weights individually and as a % of body weight and brain weight at 90 days of treatment exhibited no significant difference between the groups. No difference was observed in hematology and clinical chemistry or the histopathological evaluation. Taken together, these results show that 90 day treatment of HCA-SX results in a reduction in body weight, and does not cause any changes in major organs or in hematology, clinical chemistry, and histopathology. PMID:15228099

Shara, Michael; Ohia, Sunny E; Schmidt, Robert E; Yasmin, Taharat; Zardetto-Smith, Andrea; Kincaid, Anthony; Bagchi, Manashi; Chatterjee, Archana; Bagchi, Debasis; Stohs, Sidney J

2004-05-01

304

Activity of cyclosporins as resistance modifiers in primary cultures of human haematological and solid tumours.  

PubMed Central

The semiautomated fluorimetric microculture cytotoxicity assay (FMCA) was used for evaluation of the ability of cyclosporin A (CsA) and its novel non-immunosuppressive derivative SDZ PSC 833 (PSC) to modify the response to doxorubicin or vincristine in vitro in different haematological and solid human tumour types. Primary cultures of 322 tumour samples were analysed. Both cyclosporins showed resistance-modifying activity in all haematological tumours tested, and in solid tumours activity was observed in ovarian carcinoma and childhood tumours. Little or no effect was found in the remaining tumour types, including breast, renal and adrenal cortical carcinomas and adult sarcomas. In most of the responsive cases the interaction between the modifier and the cytotoxic drug was synergistic. There was a tendency to higher activity in samples from previously treated patients, and an inverse relationship between degree of cytotoxic drug resistance and resistance-modifying activity was noted. No difference in potency between CsA and PSC could be discerned. The results indicate differential in vitro resistance-modifying activity of the cyclosporins depending on tumour type. The results also suggest that treatment with resistance modifiers should be considered also for primary therapy of drug-sensitive tumours. Drug resistance assays such as the FMCA may become useful in preclinical evaluation of resistance modifiers.

Fridborg, H.; Jonsson, B.; Nygren, P.; Csoka, K.; Nilsson, K.; Oberg, G.; Kristensen, J.; Bergh, J.; Tholander, B.; Olsen, L.

1994-01-01

305

Guideline on the prevention of secondary central nervous system lymphoma: British Committee for Standards in Haematology.  

PubMed

The guideline group was selected to be representative of UK-based medical experts. Ovid MEDLINE, EMBASE and NCBI Pubmed were searched systematically for publications in English from 1980 to 2012 using the MeSH subheading 'lymphoma, CNS', 'lymphoma, central nervous system', 'lymphoma, high grade', 'lymphoma, Burkitt's', 'lymphoma, lymphoblastic' and 'lymphoma, diffuse large B cell' as keywords, as well as all subheadings. The writing group produced the draft guideline, which was subsequently revised by consensus by members of the Haemato-oncology Task Force of the British Committee for Standards in Haematology (BCSH). The guideline was then reviewed by a sounding board of ~50 UK haematologists, the BCSH and the British Society for Haematology (BSH) Committee and comments incorporated where appropriate. The 'GRADE' system was used to quote levels and grades of evidence, details of which can be found in Appendix I. The objective of this guideline is to provide healthcare professionals with clear guidance on the optimal prevention of secondary central nervous system (CNS) lymphoma. The guidance may not be appropriate to patients of all lymphoma sub-types and in all cases individual patient circumstances may dictate an alternative approach. Acronyms are defined at time of first use. PMID:24033102

McMillan, Andrew; Ardeshna, Kirit M; Cwynarski, Kate; Lyttelton, Matthew; McKay, Pam; Montoto, Silvia

2013-08-27

306

Haematological acclimation and re-acclimation to hypoxia in the mouse.  

PubMed

Haematological responses throughout 4w of initial acclimation (IA) and three paradigms of re-acclimation (RA) to hypoxia ( [Formula: see text] ) were examined in female mice. We hypothesised that (i) haematological responses would be increased during re-exposure, resulting in greater O2-carrying capacity in RA compared to IA; and (ii) further improvements would occur when abbreviating the de-acclimation period to 1w (RA?DA) or extending the IA period to 8w (RA?IA). The serum [EPO] response was blunted in all RA groups compared to IA but the resulting reticulocyte response was similar in all experimental groups. The [Hb] response was the same in RA and RA?DA as in IA but was blunted in RA?IA due to a reduction in mean corpuscular Hb. The sensitivity of EPO-producing cells appears blunted but the sensitivity of erythroid precursors to EPO is enhanced by recent hypoxic exposure. Erythropoietic regulation is altered during RA in a manner that is dependent on the paradigm of initial exposure. PMID:23891710

Macnutt, Meaghan J; Rupert, James L; Sheel, A William

2013-07-24

307

Haematological and blood biochemical studies in female domesticated Indian elephants (Elaphas maximus L.).  

PubMed

1. Some haematological and biochemical blood parameters in female Indian elephants were investigated. 2. Haematological data were as follows: Ht = 39.2 +/- 2.36%, Hb = 10.1 +/- 0.54 g%, RBC = 2.66 +/- 0.32 x 10(6)/mm3, WBC = 5.43 +/- 0.48 x 10(3)/mm3. Lymphocytes, determined on blood smears were mainly leucocytes from (67.0 +/- 1.59%). Data for MCV, MCH and MCHC are also given. 3. Blood plasma was separated into 5 main fractions, total plasma protein concentration was 6.98 +/- 0.53 g%, A/G ratio was 0.69 +/- 0.1. 4. Plasma minerals concentration was as follows: Na, 3044 +/- 194 micrograms/ml; K, 529 +/- 38.5 micrograms/ml; Mg, 33.0 +/- 3.43 micrograms/ml; Ca, 181.0 +/- 17.8 micrograms/ml; InP, 44.6 +/- 6.1 micrograms/ml. Ca: P ratio was 3.25 +/- 0.34. 5. Some seasonal differences in investigated parameters were observed. Ht values, WBC and neutrophils number as well as Ca, and Mg concentrations were higher during winter, whereas RBC and Na and InP concentrations were lower in winter. PMID:2896562

Gromadzka-Ostrowska, J; Jakubów, K; Zalewska, B; Krzywicki, Z

1988-01-01

308

Cell counting of body fluids: comparison between three automated haematology analysers and the manual microscope method.  

PubMed

INTRODUCTION: Haematological analysis of body fluids (BF) specimens can provide clinicians with valuable diagnostic information because it can indicate one of several serious medical conditions. Although up to now the microscopic counting and the differentiation of WBC in a BF smear have been used as a reference. The introduction of semiautomated and automated methods of analysis has reduced interoperator variability and improved turnaround time and precision. The aim of our study was to evaluate the accuracy and the correlation between the three methods and with the reference method. METHODS: We examined 110 body fluid samples. Total counting of each sample has been conducted with all systems: Pentra DX120, ADVIA 2120 and XE-2100 and the manual method. RESULTS: We found statistically significant correlation between the data obtained in the ascitic and pleuric liquid but not in the cerebrospinal fluid. CONCLUSION: The introduction of automated method for BF analysis is more and more useful in the routine job of a laboratory analysis. It is therefore very important to evaluate the performance of the different automated haematology technologies, because there is a lack of literature in this field. The comparison between the Pentra DX 120, the other technologies and the manual counting showed instrumental overlapping capabilities. PMID:23647736

Danise, P; Maconi, M; Rovetti, A; Avino, D; Di Palma, A; Gerardo Pirofalo, M; Esposito, C

2013-05-01

309

Haematological effects among silk screening workers exposed to 2-ethoxy ethyl acetate  

PubMed Central

Aims: To investigate the haematological effects in 2-EEA exposed workers. Methods: Workers from one silk screening shop (n = 29), using 2-EEA as the major cleaning and printing solvent, were recruited as a high exposure group. Workers with indirect and non-exposure to 2-EEA (n = 56) were recruited as the comparison group. Venous blood was collected for blood routine examination. Air concentration of 2-EEA in this plant was measured by eight hour personal sampling. Results: The geometric mean (GM) of air concentration of 2-EEA in the high exposure group was 7.41 ppm (range 1.35–16.5 pppm). The mean exposure of female workers (GM = 9.34 ppm) was significantly higher than that of male workers (GM = 4.87 ppm). The GM of air 2-EEA concentration in the comparison group was 0.07 ppm (range: non-detectable to 3.62 ppm, n = 26). The haemoglobin and haematocrit in the female high 2-EEA exposure workers were significantly lower than those of female workers in the comparison group. No difference was found between male 2-EEA high exposure and comparison group workers. The haemoglobin, haematocrit, and RBC count in the study population had a significant dose-response relation with air 2-EEA levels. Conclusion: Results suggest that 2-EEA is a haematological toxicant, which leads to anaemic status in high exposure female workers.

Loh, C; Shih, T; Liou, S; Lin, Y; Hsieh, A; Chen, C; Liao, G

2003-01-01

310

Prospective study of hepatic, renal, and haematological surveillance in hazardous materials firefighters  

PubMed Central

OBJECTIVES—To evaluate possible health effects related to work with hazardous materials as measured by end organ effect markers in a large cohort over about 2 years, and in a subcohort over 5 years.?METHODS—Hepatic, renal, and haematological variables were analysed from 1996-98 in hazardous materials firefighters including 288 hazardous materials technicians (81%) and 68 support workers (19%). The same end organ effect markers in a subcohort of the technicians were also analysed (n=35) from 1993-98. Support workers were considered as controls because they are also firefighters, but had a low potential exposure to hazardous materials.?RESULTS—During the study period, no serious injuries or exposures were reported. For the end organ effect markers studied, no significant differences were found between technicians and support workers at either year 1 or year 3. After adjustment for a change in laboratory, no significant longitudinal changes were found within groups for any of the markers except for creatinine which decreased for both technicians (p<0.001) and controls (p<0.01).?CONCLUSIONS—Health effects related to work are infrequent among hazardous materials technicians. Haematological, hepatic, and renal testing is not required on an annual basis and has limited use in detecting health effects in hazardous materials technicians.???Keywords: hazardous materials; firefighters; medical surveillance

Kales, S; Polyhronopoulos, G; Aldrich, J; Mendoza, P; Suh, J; Christiani, D

2001-01-01

311

ACS Green Chemistry Institute  

NSDL National Science Digital Library

This special feature page from the American Chemical Society (ACS) showcases the up-and-coming field of "green chemistry," that is, the development of chemical products and processes that eliminate or reduce the use and generation of hazardous substances. A list of principles behind green chemistry, a searchable bibliography of green chemistry references, and green chemistry links (including conferences). This page comes from ACS's Green Chemistry Project, a three-year educational project to develop and disseminate green chemistry educational materials for graduate and undergraduate chemistry students.

2005-12-13

312

Low-dose naltrexone effects on plasma chemistries and clinical symptoms in autism: a double-blind, placebo-controlled study  

Microsoft Academic Search

The effect of month-long naltrexone (NTX) treatment at a daily oral dose of 0.5 mg\\/kg\\/day was contrasted with placebo (PLC) in a double-blind study with conjoint clinical and biochemical evaluations of therapeutic effects. Modest clinical benefits were achieved with both PLC and NTX, with marginally better overall results following NTX, and degree of improvement appeared to be related to plasma

Manuel P. Bouvard; Marion Leboyer; Jean-Marie Launay; Christophe Recasens; Marie-Hélène Plumet; Delphine Waller-Perotte; François Tabuteau; Dominique Bondoux; Michel Dugas; Patrick Lensing; Jaak Panksepp

1995-01-01

313

Patients with haematological malignancies requiring invasive mechanical ventilation: differences between survivors and non-survivors in intensive care unit  

Microsoft Academic Search

Background: Mortality of patients with haematological malig- nancies requiring intensive therapy is high. We wanted to establish reasons for intensive care unit (ICU) admis- sion and treatment as well as outcome in subjects who required invasive mechanical ventilation. We were also interested in differences between ICU survivors and non-survivors at the moment of admission. Patients and methods: Forty patients (21

Radoslaw Owczuk; Magdalena A. Wujtewicz; Wioletta Sawicka; Anna Wadrzyk; Maria Wujtewicz

2005-01-01

314

Morphological and haematological parameters in the Algerian mouse ( Mus spretus) inhabiting an area contaminated with heavy metals  

Microsoft Academic Search

In the present study morphological (ratio of body weight to body length, weight of internal organs) and haematological parameters (cells count, haemoglobin concentration, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration) were used for monitoring potential effects of metal contaminants on the Algerian mouse (Mus spretus). The study also provided the opportunity to evaluate the use of

A. C Nunes; M da Luz Mathias; A. M Crespo

2001-01-01

315

The investigation of parvovirus B19 infection in patients with haematological disorders by using PCR and ELISA techniques  

Microsoft Academic Search

Parvovirus B19 has a marked tropism for erythroid progenitor cells. This may lead to chronic anemia in predisposed individuals. The purpose of the study was to investigate the frequency of parvovirus B19 infections in patients with diagnosis of haematological disorders. In order to determine the diagnostic use of different markers of parvovirus B19 infection, serum specimens obtained from 79 patients

Tercan Us; Leyla Ozunel; Nilgun Kasifoglu; Yurdanur Akgun

2007-01-01

316

The control of hyperendemic glycopeptide-resistant Enterococcus spp. on a haematology unit by changing antibiotic usage  

Microsoft Academic Search

The rectal carriage of glycopeptide-resistant Enterococcus spp. (GRE) had been established at approximately 50% in a series of prevalence studies on a busy haematological malignancy unit. The aim of this study was to reduce the chance of patients acquiring GRE. A prospective three- phase sequential study was performed. In Phase 1, the acquisition rate of GRE detectable by rectal swab

Susan J. Bradley; Angela L. T. Wilson; Michael C. Allen; Hilary A. Sher; Anthony H. Goldstone; Geoffrey M. Scott

1999-01-01

317

Integrating a Primer Course in Biostatistics into the Haematology Practicals of First-Year Medical Students in India  

Microsoft Academic Search

Context: In India, biostatistics in the medical curriculum is usually taught as a separate course, using either ``imaginary'' data or data from the literature. Knowledge of statistics is particularly important in the context of ``evidence-based medicine''. Objective: To evaluate the ef® cacy of a biostatistics course integrated into the practical haematology ® rst-year medical course with the following special characteristics:

Mario Vaz

2001-01-01

318

Some Biochemical, Haematological and Histological Responses to a Long Term Consumption of Telfairia occidentalis-Supplemented Diet in Rats  

Microsoft Academic Search

2 Abstract: Some biochemical, haematological and histological responses were studied in rats undergoing a long term feeding with a Telfairia occidentalis-supplemented diet. Biochemical and hematological parameters investigated included serum protein, total cholesterol, lipid peroxidation, haemoglobin, white blood cells, Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase, Glutathione-s- transferase and Superoxide dismutase. Histological changes associated with Telfairia occidentalis- supplemented diet on the liver,

Onyechi Obidoa

2009-01-01

319

Phase Ib clinical trial of starch-conjugated deferoxamine (40SD02): a novel long-acting iron chelator.  

PubMed

The most widely used drug for iron chelation is deferoxamine (DFO) mesylate. While effective in promoting iron excretion, it requires prolonged daily infusions, often resulting in poor compliance. A clinical trial was conducted using starch-conjugated DFO (S-DFO; 40SD02), a high-molecular-weight iron chelator possessing prolonged vascular retention. Single doses of S-DFO were infused intravenously into groups of four transfusion-dependent patients with beta-thalassaemia at doses of 150, 300, 600 and 900 mg/kg. Urinary iron excretion and various pharmacologic parameters were evaluated for 1 week and safety for 3 weeks. No drug-related effects were observed on clinical chemistries, haematological and coagulation parameters, urinalyses, vital signs or electrocardiograms. Drug-related adverse events were limited to four urticarial reactions, none requiring termination of the infusion. The drug stimulated clinically significant urinary iron excretion, with the highest dose (900 mg/kg) inducing excretion of 1.31 mg of iron/kg (range 0.79-1.90 mg/kg) over 1 week, with residual iron-binding capacity present in the plasma for over 6 d. In summary, treatment with S-DFO, administered weekly, has the potential to achieve iron balance in the poorly compliant patient. PMID:17614825

Harmatz, Paul; Grady, Robert W; Dragsten, Paul; Vichinsky, Elliott; Giardina, Patricia; Madden, Jacqueline; Jeng, Michael; Miller, Becky; Hanson, Gregory; Hedlund, Bo

2007-08-01

320

Safety evaluation of daidzein in laying hens: part I. Effects on laying performance, clinical blood parameters, and organs development.  

PubMed

Daidzein, an estrogen-like product, becomes increasingly popular as a dietary supplement, particularly for postpeak-estrus animals seeking a safe natural alternative to play a role of estrogen. However, there is little available safety data of it for raisers and consumers. A subchronic laying hen safety study was conducted to examine if the high-dose daidzein could affect the safety of hens selves, including laying performance, clinical blood parameters and organs development. Seven hundred and sixty-eight 56-week-old Hyline Brown were randomly assigned to 4 groups with 8 replicates of 24 birds each and 3weeks later fed diets supplemented with 0, 10, 50 and 100mg of daidzein/kg for 12weeks. The mortality was significantly decreased (P<0.05). No treatment related adverse clinical signs were observed. Mean egg production, egg mass and feed conversion of whole experiment period was significantly influenced by dietary daidzein supplement (P<0.05), showing significant quadratic response to increasing dietary daidzein supplement (P=0.029, P=0.003 and P=0.019, respectively). There was no statistically significant changes in haematology (P>0.05). In clinical chemistry parameters, total protein, total cholesterol, calcium and phosphorus were significantly affected by dietary daidzein supplement (P<0.05). The no observed adverse effect level (NOAEL) is considered to be 50mg/kg. PMID:23391597

Shi, S R; Gu, H; Chang, L L; Wang, Z Y; Tong, H B; Zou, J M

2013-02-04

321

Method for detection of bromine in urine using liquid chemistry dry chemistry test pads and lateral flow  

US Patent & Trademark Office Database

This invention is in the field of toxicology and clinical diagnostics. More specifically, this invention provides a single dry chemistry, liquid chemistry, or lateral flow dry chemistry combination test device for use in the detection of adulteration by the addition of bromine(s) to a specimen submitted for Drugs of Abuse (DAU) testing and clinical diagnostic purposes in aqueous fluids, including urine, saliva, serum, blood, sweat extracts, and liquid homogenates of hair.

Smith; Jack V. (Asheville, NC)

2003-03-25

322

Analysis of some biochemical and haematological parameters for Mucuna pruriens (DC) seed powder in male rats.  

PubMed

The biochemical and haematological effects of the seed powder of Mucuna pruriens in male rats were evaluated to establish some biological properties of this potential biopesticide currently undergoing investigation. The result showed that Mucuna pruriens seed extract produced a significant (p<0.05) increase in white blood cell (WBC) count, as well as in bilirubin concentrations, alkaline phosphatase (ALP), protein and creatinine levels measured. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly reduced (P<0.05) in comparison with the experimental control. PCV, Hb, albumin level and WBC differential counts gave no significant difference between treated and control groups. The results revealed metabolic imbalance in the rats which suggests a mild cholestasis effect of the extract. PMID:21959815

Chukwudi, Ndukwe Henry; Simeon, Omale; Chinyere, Aguiyi John

2011-10-01

323

Impact of HPAI H5N1 infection on select blood chemistry and gas ranges in layers, broilers and turkeys using the i-STAT Clinical Analyzer  

Technology Transfer Automated Retrieval System (TEKTRAN)

Infection of poultry with highly pathogenic avian influenza (HPAI) H5N1 typically results in mortality within six days of inoculation. However, the exact mechanisms that induce death are unknown, even as birds may die in the absence of overt clinical signs of disease. In human and many veterinary ...

324

The effects of probiotic and mannanoligosaccharide on some haematological and immunological parameters in turkeys.  

PubMed

The study was conducted to investigate the effects of mannanoligosaccharide (MOS) and probiotic supplementation on haematological and immunological parameters in turkeys. A total of 72, 15-day-old white hybrid converter turkey poults were used in this study. Poults were assigned into three groups, each group consisted of six poults and the trial was repeated four times. The control group was fed a basal diet without supplemented probiotic and MOS, and treatment groups were fed either 1 g/kg MOS or probiotic added diets. At the end of the 15-week treatment period, samples of blood were collected to determine immunological and haematological values. The comparison among the groups showed that both the probiotic and MOS supplementation resulted in significant increases (P < 0.05) in the serum IgG and IgM levels, and significant decreases (P < 0.05) in the peripheral blood T lymphocyte percentage compared with those of the control group. Mean serum IgG level (7.06 g/l) was significantly higher (P < 0.05) in poults supplemented with probiotic compared with MOS (6.76 g/l). It was observed that the probiotic supplementation caused statistically significant increases (P < 0.05) in the erythrocyte count, haemoglobin concentration and haematocrit values, but MOS supplementation did not have a significant effect (P > 0.05) on these parameters. Total leucocyte and differential leucocyte counts were not affected by dietary MOS and probiotic supplementation. These results show that MOS or probiotic may elevate IgG and IgM levels in turkey. The MOS and probiotic that enhance immunoglobulin levels will have a more positive effect on growth performance, production and turkeys' ability to resist disease. PMID:16050905

Cetin, N; Güçlü, B K; Cetin, E

2005-08-01

325

Constitutional dynamic chemistry: bridge from supramolecular chemistry to adaptive chemistry.  

PubMed

Supramolecular chemistry aims at implementing highly complex chemical systems from molecular components held together by non-covalent intermolecular forces and effecting molecular recognition, catalysis and transport processes. A further step consists in the investigation of chemical systems undergoing self-organization, i.e. systems capable of spontaneously generating well-defined functional supramolecular architectures by self-assembly from their components, thus behaving as programmed chemical systems. Supramolecular chemistry is intrinsically a dynamic chemistry in view of the lability of the interactions connecting the molecular components of a supramolecular entity and the resulting ability of supramolecular species to exchange their constituents. The same holds for molecular chemistry when the molecular entity contains covalent bonds that may form and break reversibility, so as to allow a continuous change in constitution by reorganization and exchange of building blocks. These features define a Constitutional Dynamic Chemistry (CDC) on both the molecular and supramolecular levels.CDC introduces a paradigm shift with respect to constitutionally static chemistry. The latter relies on design for the generation of a target entity, whereas CDC takes advantage of dynamic diversity to allow variation and selection. The implementation of selection in chemistry introduces a fundamental change in outlook. Whereas self-organization by design strives to achieve full control over the output molecular or supramolecular entity by explicit programming, self-organization with selection operates on dynamic constitutional diversity in response to either internal or external factors to achieve adaptation.The merging of the features: -information and programmability, -dynamics and reversibility, -constitution and structural diversity, points to the emergence of adaptive and evolutive chemistry, towards a chemistry of complex matter. PMID:22169958

Lehn, Jean-Marie

2012-01-01

326

Collaborative Physical Chemistry Projects Involving Computational Chemistry  

Microsoft Academic Search

The physical chemistry classes from three colleges have collaborated on two computational chemistry projects using Quantum CAChe 3.0 and Gaussian 94W running on Pentium II PCs. Online communication by email and the World Wide Web was an important part of the collaboration. In the first project, students used molecular modeling to predict benzene derivatives that might be possible hair dyes.

David M. Whisnant; Jerry J. Howe; Lisa S. Lever

2000-01-01

327

Special Report: Brain Chemistry.  

ERIC Educational Resources Information Center

Chemical actions in the brain result in cognitive, emotional, neuroendocrine, neuromuscular, and/or neurocirculatory effects. Developments in understanding brain chemistry are discussed, considering among others, neurotransmitter chemistry, neuropeptides, drugs and the brain, antidepressants, and actions of minor tranquilizers. (JN)

Krassner, Michael B.

1983-01-01

328

Chemistry of Americium.  

National Technical Information Service (NTIS)

Essential features of the descriptive chemistry of americium are reviewed. Chapter titles are: discovery, atomic and nuclear properties, collateral reading, production and uses, chemistry in aqueous solution, metal, alloys, and compounds, and, recovery, s...

W. W. Schulz

1976-01-01

329

Environmental chemistry: Volume A  

SciTech Connect

This is an extensive introduction to environmental chemistry for engineering and chemical professionals. The contents of Volume A include a brief review of basic chemistry prior to coverage of litho, atmo, hydro, pedo, and biospheres.

Yen, T.F.

1999-08-01

330

Aquatic Chemistry, Third Edition  

NASA Astrophysics Data System (ADS)

Most water scientists and environmental engineers will recognize this classic environmental chemistry text as “Stumm and Morgan” rather than Aquatic Chemistry. Publication of the first edition in 1970 established a standard for books dealing with environmental chemistry in terms of depth and breadth of coverage. Scientists and engineers concerned with issues of water quality are well advised to have Aquatic Chemistry as part of their personal libraries, or at least in their office libraries.

Schroeder, Edward D.; Young, Thomas M.

331

Mechanisms in Photographic Chemistry  

ERIC Educational Resources Information Center

Reviews current research interests in photographic chemistry, involving two proposed models for spectral sensitization of crystal defects and impurities in the photolysis reactivity and the mechanisms of development and complexation. Establishment of photographic chemistry in a chemistry curriculum is recommended. (CC)

Sahyun, M. R. V.

1974-01-01

332

Green Chemistry and Education.  

ERIC Educational Resources Information Center

|Many students today are profoundly interested in the sustainability of their world. Introduces Green Chemistry and its principles with teaching materials. Green Chemistry is the use of chemistry for pollution prevention and the design of chemical products and processes that are environmentally benign. (ASK)|

Hjeresen, Dennis L.; Schutt, David L.; Boese, Janet M.

2000-01-01

333

Green Chemistry and Education.  

ERIC Educational Resources Information Center

Many students today are profoundly interested in the sustainability of their world. Introduces Green Chemistry and its principles with teaching materials. Green Chemistry is the use of chemistry for pollution prevention and the design of chemical products and processes that are environmentally benign. (ASK)

Hjeresen, Dennis L.; Schutt, David L.; Boese, Janet M.

2000-01-01

334

Mechanisms in Photographic Chemistry  

ERIC Educational Resources Information Center

|Reviews current research interests in photographic chemistry, involving two proposed models for spectral sensitization of crystal defects and impurities in the photolysis reactivity and the mechanisms of development and complexation. Establishment of photographic chemistry in a chemistry curriculum is recommended. (CC)|

Sahyun, M. R. V.

1974-01-01

335

Chemistry on Stamps.  

ERIC Educational Resources Information Center

|Suggests how postage stamps can be incorporated into chemistry teaching. Categories considered include emergence of chemistry as a science, metric system, atoms (and molecules and ions), stoichiometry, energy relationships in chemical systems, chemical bonding, nuclear chemistry, biochemistry, geochemistry, matter (gases, liquids, and solids),…

Schreck, James O.

1986-01-01

336

Aquatic Chemistry, Third Edition  

Microsoft Academic Search

Most water scientists and environmental engineers will recognize this classic environmental chemistry text as ``Stumm and Morgan'' rather than Aquatic Chemistry. Publication of the first edition in 1970 established a standard for books dealing with environmental chemistry in terms of depth and breadth of coverage. Scientists and engineers concerned with issues of water quality are well advised to have Aquatic

Edward D. Schroeder; Thomas M. Young

1996-01-01

337

Analytical chemistry instrumentation  

Microsoft Academic Search

Separate abstracts were prepared for 48 papers in these conference proceedings. The topics covered include: analytical chemistry and the environment; environmental radiochemistry; automated instrumentation; advances in analytical mass spectrometry; Fourier transform spectroscopy; analytical chemistry of plutonium; nuclear analytical chemistry; chemometrics; and nuclear fuel technology. (LEW)

Laing

1986-01-01

338

A Label-Free Porous Silicon Immunosensor for Broad Detection of Opiates in a Blind Clinical Study and Result Comparison to Commercial Analytical Chemistry Techniques  

PubMed Central

In this work we evaluate for the first time the performance of a label-free porous silicon (PSi) immunosensor assay in a blind clinical study designed to screen authentic patient urine specimens for a broad range of opiates. The PSi opiate immunosensor achieved 96% concordance with liquid chromatography-mass spectrometry/tandem mass spectrometry (LC-MS/MS) results on samples that underwent standard opiate testing (n=50). In addition, successful detection of a commonly abused opiate, oxycodone, resulted in 100% qualitative agreement between the PSi opiate sensor and LC-MS/MS. In contrast, a commercial broad opiate immunoassay technique (CEDIA®) achieved 65% qualitative concordance with LC-MS/MS. Evaluation of important performance attributes including precision, accuracy, and recovery was completed on blank urine specimens spiked with test analytes. Variability of morphine detection as a model opiate target was < 9% both within-run and between-day at and above the cutoff limit of 300 ng ml?1. This study validates the analytical screening capability of label-free PSi opiate immunosensors in authentic patient samples and is the first semi-quantitative demonstration of the technology’s successful clinical use. These results motivate future development of PSi technology to reduce complexity and cost of diagnostic testing particularly in a point-of-care setting.

Bonanno, Lisa M.; Kwong, Tai C.; DeLouise, Lisa A.

2010-01-01

339

Alcohol, Chemistry and You  

NSDL National Science Digital Library

Developed by Kennesaw State University, ChemCases.com is a series of curriculum units that link responsible decision making in product development with chemical principles taught in college General Chemistry. Alcohol, Chemistry and You, by Dr. Bill Boggan, is the latest offering by the Web site, which "looks at the chemistry of beverage alcohol (ethyl alcohol) through the eyes of a General Chemistry student." The fourteen chapter lessons cover everything from what ethyl alcohol is to alcohol addiction, relating it to various principles learned in a general chemistry course.

2001-01-01

340

Platelet count evaluation using three automated haematology analysers compared with the immunoplatelet reference method, and estimation of possible inadequate platelet transfusion.  

PubMed

The accuracy of three automated haematology analysers [Sysmex XE-2100 (both optical and impedance mode), Bayer Advia 120, and Beckman Coulter LH-750] was compared with the immunoplatelet reference method for platelet measurement. A total of 165 blood specimens were obtained from patients and platelet counts were determined using the four-automated haematology analyser methods and the immunoplatelet reference method. The coefficients of determination (R(2)) between the automated haematology analyser methods and the immunoplatelet reference method for the overall platelet range were >0.98. A bias study, however, showed some disagreement. The use of a coincidence correction calculation for the immunoplatelet method did not improve the correlation between the immunoplatelet method and the automated haematology analyser methods. To estimate the possibility of inadequate platelet transfusion, the number of prophylactic platelet transfusion indications determined by the automated haematology analyser platelet counts were compared with the number of transfusion indications according to the platelet counts determined by the immunoplatelet method. An additional 48 blood specimens were included in this analysis. All of the automated haematology analysers showed some disagreement in the transfusion indications when compared with the immunoplatelet method, suggesting the possibility of inadequate platelet transfusion. PMID:18294237

Hong, K H; Kim, M J; Lee, K W; Park, K U; Kim, H S; Song, J

2008-02-21

341

The Effect of Cement Dust Exposure on Haematological Parameters of Cement Factory workers in Nalagonda, Andhra Pradesh.  

NASA Astrophysics Data System (ADS)

This study was measured on haematological parameters in workers exposed to cement dust in order to test the the hypothesis and to identify a simple, readily available, cost effective screening test that could help in identifying the presence of disease, its severity, that Cement dust exposure may perturb these functions related to their workplace.Assesment of haematological parameters were performed in 100exposed workers occupationally exposed to cement dust and 50 matched unexposed controls with ages ranging from 20-35, 35-50, 50-65 years. The blood samples were taken from them and percentage of hemoglobin, Lymphocytes / monocytes count were analysed.The hemoglobin percentage of exposed workers were significantly lower(P<0.05).Lymphocytes/Monocytes counts of exposed workers was insignificant (P<0.05).These results suggest that long term occupational exposure to cement dust may perturb haemopoietic function.

Guguloth, Mohan Rao.; Sambanaik, A.; srinivasnaik, L.; Mude, Jagadishnaik.

2012-10-01

342

Effect of 12-hour road transportation on physiological, immunological and haematological parameters in bulls housed at different space allowances  

Microsoft Academic Search

The effects of transporting Holstein Friesian bulls (n=72; bodyweight 403±3.5kg) for 12h by road were examined. Adrenal, haematological and immune responses, body temperature and performance were recorded. The animals had been previously housed for 96 days at three space allowances (1.2, 2.7 or 4.2m2 per bull). The bulls were allocated to one of two treatments: T (transport for 12h; n=16

S. Gupta; B. Earley; M. A. Crowe

2007-01-01

343

Nitrite toxicity in Cirrhinus mrigala (Ham.): acute toxicity and sub-lethal effect on selected haematological parameters  

Microsoft Academic Search

The acute toxicity and primary stress effect of the sub-lethal concentrations of nitrite (NO2?) on certain haematological parameters were studied in static bioassay systems in fingerlings of mrigal, Cirrhinus mrigala (Ham.), a species of major importance for aquaculture in India. The 96-h LC50 was 10.4 mg l?1. In sub-lethal test, the changes in the total erythrocyte count (TEC), total leukocyte

P. C. Das; S. Ayyappan; J. K. Jena; B. K. Das

2004-01-01

344

Growth performance, haematology and serum biochemistry of African catfish ( Clarias gariepinus ) fingerlings fed graded levels of dietary fumonisin B 1  

Microsoft Academic Search

Fingerlings of Clarias gariepinus were used to evaluate the effect of dietary fumonisin B1 (FB1), a mycotoxin produced by Fusarium verticillioides, on growth, haematological and serum biochemical parameters. The fingerlings were sorted, weighed and randomly stocked in\\u000a 16 plastic tanks at the rate of 20 fingerlings per tank. Fusarium-cultured maize grains containing FB1 were used to formulate three diets containing

Francis A. Gbore; Adeyemo M. Adewole; Olatunde Oginni; Mercy F. Oguntolu; Ayodeji M. Bada; Olatunbosun Akele

2010-01-01

345

Effects of ethanolic extracts of Garcinia kola seeds on growth and haematology of catfish (Clarias gariepinus) broodstock  

Microsoft Academic Search

A 56 day study was undertaken to evaluate the effects of dietary ethanolic extracts of Garcinia kola (Bitter kola) in African catfish, Clarias gariepinus broodstock on growth performance and basic haematological indices. Catfish broodstock (mean weight, 245.20 - 255.00 g) were randomly distributed into concrete tanks (2 x 2 x 1.2 m) at 10 fish\\/tank in triplicate treatments. 5 diets

A. A. Dada; M. Ikuerowo

346

Haematological and Biochemical Changes in Japanese Quails Coturnix coturnix Japonica and Chickens Due to Ascaridia galli Infection  

Microsoft Academic Search

2 Abstract: A study was carried out in Japanese quails Coturnix coturnix japonica and chickens (Rhode Island Red) for comparative haematological and biochemical changes occurring due to Ascaridia galli infection. Quails as well as chickens were divided into four small groups, each consisting six numbers of birds in each group and were marked as q1, q2, q3, qC and p1,

K. Deka; J. Borah

2008-01-01

347

A randomised, prospective comparison of allogeneic bone marrow and peripheral blood progenitor cell transplantation in the treatment of haematological malignancies  

Microsoft Academic Search

We present the results of a prospective, randomised study comparing PBPC and BM focusing on engraftment, acute and chronic GVHD and survival. Forty patients with haematological malignancies received HLA-identical sibling BM (group A) or PBPC (group B). Evaluable patients were 19 (A) and 18 (B). Median age was 35 (17–56) in A and 29.5 (9–51) in B. Conditioning was mainly

AC Vigorito; WM Azevedo; JFC Marques; AM Azevedo; KAB Eid; FJP Aranha; I Lorand-Metze; GB Oliveira; MEP Correa; ARC Reis; ECM Miranda; CA de Souza

1998-01-01

348

Role of Haematological Parameters as an Indicator of Acute Malarial Infection in Uttarakhand State of India  

PubMed Central

Background Malaria may be associated with complications which may be avoided by early diagnosis and treatment. Microscopic diagnosis showing presence of malarial parasites is needed for confirmation which at times may be unreliable and requires technical expertise. The present study was conducted to statistically analyze the haematological parameters including platelet indices which can give initial hint for malarial infection and therefore prompt the laboratory physician for active search of the parasite microscopically. Methods A retrospective study was conducted which included 334 cases of acute malaria caused by Plasmodium vivax, falciparum and dual infection along with 100 cases of control. Routine haematological parameters along with platelet indices (MPV and PDW) which are easily available on automated cell counter were statistically analyzed to assess their role as indicators for malaria. Results Leukocyte count and platelet count were significantly lower in cases of acute malaria in comparison to controls (p value <0.001). Platelet count<150×109/l showed 87.2% sensitivity, 65% specificity, 89.3% positive predictive value and 2.49 likelihood ratio for the infection. PDW of 6–10 and MPV>8 fl had 71.9% and 61.5% sensitivity and 78.2% and 77.7% positive predictive value respectively for infection. Platelet count <150×109/l and MPV>8 fl was comparatively more sensitive indicator for vivax (88% and 70.8% respectively) than falciparum (84.8% and 50.4% respectively) and PDW 6–10 was more sensitive indicator for falciparum (82.6%) than vivax (69.5%) infection. Conclusion Thrombocytopenia (<150×109/l) and low leukocyte count (<4×109/l) may be used as probable indicator for malaria in endemic countries. Higher MPV (>8 fl) and PDW of 6–10 also show considerable sensitivity for malarial infection. In addition, thrombocytopenia (<150×109/l) and higher MPV (>8 fl) was more sensitive for vivax infection while PDW 6–10 was more sensitive for falciparum infection.

Chandra, Smita; Chandra, Harish

2013-01-01

349

The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs  

PubMed Central

Background The abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Tannin is more natural and cheaper but possibly with the same effectiveness as organic acids with regard to growth performance. Methods Thirty-six 7 week old grower pigs, divided into three equal groups, were used in a three week feeding trial. Group I was fed basal diet, group II basal diet with added organic acids and group III basal diet with added organic and inorganic acids and tannin. Pigs were weighed before and after feeding and observed daily. Blood was collected before and after the feeding trial for the determination of selected biochemical, haematological and antioxidant enzyme parameters. One-way ANOVA was used to assess any diet related changes of all the parameters. Paired t-test was used to evaluate changes of blood parameters individually in each group of growers before and after feeding. Results No clinical health problems related to diet were noted during the three week feeding trial. The average daily gain (ADG) and selected blood parameters were not affected by the addition to basal diet of either acids and tannin or of organic acids alone. Selected blood parameters remained within the reference range before and after the feeding trial, with the exception of total serum proteins that were below the lower value of reference range at both times. The significant changes (paired t-test) observed in individual groups before and after the feeding trial are related to the growth of pigs. Conclusion Diet with acids and tannin did not improve the growth performance of grower pigs but had no deleterious effects on selected blood parameters. The possibility of beneficial effects of adding acids and tannin in diets on growth performance over a longer period, however, could not be excluded.

2010-01-01

350

Outbreak of Multi-Drug Resistant Pseudomonas aeruginosa Bloodstream Infection in the Haematology Unit of a South African Academic Hospital  

PubMed Central

Objective To describe an outbreak of multi-resistant Pseudomonas aeruginosa bloodstream infections (MRPA-BSI) that occurred in the haematology ward of a tertiary academic hospital in Cape Town, South Africa, and determine risk factors for acquisition of MRPA-BSI. Methods The outbreak investigation included a search for additional cases, review of patient records, environmental and staff screening, molecular typing using pulsed-field gel electrophoresis (PFGE) and Multi-locus sequencing (MLST) and a retrospective case-control study. Results Ten MRPA-BSI cases occurred in the haematology ward between January 2010 and January 2011. The case fatality rate was 80%. Staff screening specimens were negative for MRPA and an environmental source was not identified. PFGE showed that 9/10 isolates were related. MLST showed that 3 of these 9 isolates belonged to Sequence type (ST) 233 while the unrelated isolate belonged to ST260. Conclusion We have described an outbreak of MRPA-BSI occurring over an extended period of time among neutropenic haematology patients. Molecular typing confirms that the outbreak was predominantly due to a single strain. The source of the outbreak was not identified, but the outbreak appears to have been controlled following intensive infection control measures.

Mudau, Maanda; Jacobson, Rachael; Minenza, Nadia; Kuonza, Lazarus; Morris, Vida; Engelbrecht, Heather; Nicol, Mark P.; Bamford, Colleen

2013-01-01

351

Effect of Aqueous Extract of Telfairia occidentalis Leaf on the Performance and Haematological Indices of Starter Broilers  

PubMed Central

This experiment was conducted to determine the effect of aqueous extract of Telfairia occidentalis (Fluted Pumpkin) leaf on the performance and haematological indices of starter broilers. A total of 200, 8-day-old broiler chicks were randomly allotted to five (5) treatments, each with 4 replicate groups containing 10 chicks and fed with standard starter broiler diets. Telfaria occidentalis leaves extract (FPLE) was added at 0, 40, 80, 120, and 160?mL/litre of drinking water. Growth performance and haematological indices were evaluated. Results showed that there was significant (P < 0.05) difference in weight gain, feed conversion, and protein efficiency ratios of the birds among the treatments. Birds fed 80?ml FPLE/litre of water had significantly the highest weight gain and the best feed conversion and protein efficiency ratios. There was no significant (P > 0.05) variations in the feed and water intakes of the birds. Results also show no significant (P > 0.05) difference in haematological indices of birds among the treatments. The results of this study indicate that, for enhanced weight gain and feed conversion efficiency, birds should be fed 80?mL FPLE/litre of water.

P. N., Onu

2012-01-01

352

Medical and Clinical Laboratory Technologists and Technicians  

MedlinePLUS

... blood and its components for transfusions. Clinical chemistry technologists prepare specimens and analyze the chemical and ... the medical laboratory sciences should take courses in chemistry, biology, and mathematics. Universities and hospitals offer medical ...

353

Central venous catheter-associated bloodstream infection and colonisation of insertion site and catheter tip. What are the rates and risk factors in haematology patients?  

PubMed

Skin colonisation is an important source for central venous catheter (CVC) colonisation and infection. This study intended to identify risk factors for skin colonisation prior to CVC placement (baseline colonisation) and within 10 days after CVC insertion (subsequent colonisation), for CVC-tip colonisation and for bloodstream infection (BSI). Within a randomised clinical trial, data of 219 patients with haematological malignancies and inserted CVC (with a total of 5,501 CVC-days and 4,275 days at risk) in two university hospitals were analysed. Quantitative skin cultures were obtained from the insertion site before CVC placement and at regular intervals afterwards. CVC-tip cultures were taken on CVC removal and data collection was performed. Statistical analysis included linear and logistic regression models. Age was an independent risk factor for colonisation prior to CVC placement (baseline colonisation). Independent risk factors for subsequent colonisation were baseline colonisation and male gender. High level of subsequent skin colonisation at the insertion site was a predictor of CVC-tip colonisation, and a predictor of BSI. High level of skin colonisation predicts catheter tip colonisation and possibly subsequent infection. Sustained reduction of bacterial growth at the CVC insertion site is therefore indispensable. Male patients are at particular risk for skin colonisation and may be a target population for additional insertion-site care before and during catheterisation. PMID:20532506

Luft, Dirk; Schmoor, Claudia; Wilson, Christine; Widmer, Andreas F; Bertz, Hartmut; Frei, Reno; Heim, Dominik; Dettenkofer, Markus

2010-06-08

354

Effect of azadirachtin on haematological and biochemical parameters of Argulus-infested goldfish Carassius auratus (Linn. 1758).  

PubMed

Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1-5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L(-1) as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2% DMSO solution without azadirachtin and considered as negative control T0(-). Along with six treatment groups, a positive control T0(+) of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p < 0.05) high in negative control group when compared with positive control group. It could be concluded that Argulus infestation altered marked haematological and serum biochemical parameters. However, in treated groups complete elimination of Argulus was found in T4 and T5 groups. Also significant (p < 0.05) reduction in haematological and serum biochemical parameters of all the treatment groups were recorded in comparison with negative control group. In addition, T4 and T5 groups showed significantly (p < 0.05) high superoxide dismutase (SOD), catalase, total erythrocyte count (TEC) and haemoglobin (Hb). However, higher mean corpuscular haemoglobin concentration (MCHC), blood glucose and lactate dehydrogenase (LDH) levels in T5 group revealed that higher concentration of azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L(-1) and that also shifted haematological and serum biochemical parameters towards homeostasis. PMID:23090629

Kumar, Saurav; Raman, R P; Kumar, Kundan; Pandey, P K; Kumar, Neeraj; Mallesh, B; Mohanty, Snatashree; Kumar, Abhay

2012-10-23

355

Assessment of haematological parameters in HIV-infected and uninfected Rwandan women: a cross-sectional study  

PubMed Central

Objectives Although haematological abnormalities are common manifestations of HIV infection, few studies on haematological parameters in HIV-infected persons have been undertaken in sub-Saharan Africa. The authors assessed factors associated with haematological parameters in HIV-infected antiretroviral-naïve and HIV-uninfected Rwandan women. Study design Cross-sectional analysis of a longitudinal cohort. Setting Community-based women's associations. Participants 710 HIV-infected (HIV+) antiretroviral-naïve and 226 HIV-uninfected (HIV?) women from the Rwanda Women's Interassociation Study Assessment. Haematological parameters categorised as (abnormal vs normal) were compared by HIV status and among HIV+ women by CD4 count category using proportions. Multivariate logistic regression models using forward selection were fit. Results Prevalence of anaemia (haemoglobin (Hb) <12.0?g/dl) was higher in the HIV+ group (20.5% vs 6.3%; p<0.001), and increased with lower CD4 counts: ?350 (7.6%), 200–349 (16%) and <200 cells/mm3 (32.2%). Marked anaemia (Hb <10.0?g/dl) was found in 4.2% of HIV+ and none of the HIV? women (p<0.001), and was highest in HIV+ women with CD4 <200 cells/mm3 (8.4%). The HIV+ were more likely than HIV? women (4.2 vs 0.5%, respectively, p=0.002) to have moderate neutropenia with white blood cells <2.0×103 cells/mm3 and 8.4% of HIV+ women with CD4 <200 cells/mm3 had moderate neutropenia. In multivariate logistic regression analysis, BMI (OR 0.87/kg/m2, 95% CI 0.82 to 0.93; p<0.001), CD4 200–350 vs HIV? (OR 3.59, 95% CI 1.89 to 6.83; p<0.001) and CD4 <200 cells/mm3 vs HIV? (OR 8.09, 95% CI 4.37 to 14.97; <0.001) had large independent associations with anaemia. There were large independent associations of CD4 <200 cells/mm3 vs HIV? (OR 7.18, 95% CI 0.78 to 65.82; p=0.081) and co-trimoxazole and/or dapsone use (OR 5.69, 95% CI 0.63 to 51.45; p=0.122) with moderate neutropenia. Conclusions Anaemia was more common than neutropenia or thrombocytopenia in the HIV-infected Rwandan women. Future comparisons of haematological parameters in HIV-infected patients before and after antiretroviral therapy initiation are warranted.

Munyazesa, Elisaphane; Emile, Ivan; Mutimura, Eugene; Hoover, Donald R; Shi, Qiuhu; McGinn, Aileen P; Musiime, Stephenson; Muhairwe, Fred; Rutagengwa, Alfred; Dusingize, Jean Claude; Anastos, Kathryn

2012-01-01

356

Serum selenium concentration at diagnosis and outcome in patients with haematological malignancies.  

PubMed

We have previously reported presentation serum selenium level to be predictive of outcome in diffuse large B-cell lymphoma. This has now been studied in a further 430 patients, 163 with acute myeloid leukaemia (AML), 156 with Hodgkin Lymphoma (HL), and 111 with Follicular Lymphoma (FL). Serum selenium was below the UK normal reference range in 45% of patients, and correlated with serum albumin (r=0·24-0·46, P<0·001-0·003) in all tumour types. Independent predictors of presentation selenium were; French-American-British subtype and albumin (P<0·001 for both) in AML, haemoglobin (P=0·002) and B-symptoms (P=0·01) in HL, and albumin (P<0·001) in FL. In AML and HL, response to first line therapy was lower in patients with low serum selenium, but selenium was no longer predictive of response when other variables were entered into a multivariate model. Low selenium was also associated with a worse overall survival in FL [Hazard Ratio (HR) 2·3, 95% confidence interval (CI) 1·4, 4·0] and a trend to a worse overall survival in AML (HR 1·43, 95% CI 0·96, 2·13) by univariate Cox regression analysis, but not by multivariate analysis. In conclusion, low serum selenium is associated with a worse outcome in patients with haematological malignancies, but is not independently predictive, suggesting that it reflects other factors. PMID:21770918

Stevens, Jane; Waters, Rachel; Sieniawska, Christina; Kassam, Shireen; Montoto, Silvia; Fitzgibbon, Jude; Rohatiner, Ama; Lister, Andrew; Joel, Simon

2011-07-20

357

Herpes simplex virus and intraoral ulcers in immunocompromised patients with haematologic malignancies.  

PubMed

Possible factors predisposing to the development of intraoral ulcers in immunocompromised patients with haematologic malignancies were investigated. Among 46 patients undergoing antineoplastic treatment, 18 developed an intraoral ulcer during the prospective study. Patients with or without ulcers were comparable with respect to underlying disease, presence of teeth, the qualitative composition of the aerobic and facultatively anaerobic oral microflora, herpes simplex virus (HSV) titer positivity, a past history of herpes labialis, leukocyte and thrombocyte counts, and duration of fever. Viral cultures from saliva revealed an association between the presence of HSV in saliva and the presence of intraoral ulcers. HSV was not isolated from the saliva of any patient without ulcers. Viral cultures from the ulcers revealed growth of HSV in 11 (61%) of the 18 patients with ulcers. HSV-positive ulcers were located more often on the alveolar process than elsewhere in the oral cavity. Ulcers on the lateral borders of the tongue and on the buccal mucosa were found only in dentate patients. Development of intraoral ulcers was not associated with an increase of the HSV titer in serum in any patient. It is concluded that some intraoral ulcers, especially when located on the alveolar process, are associated with and probably caused by HSV, while mechanical trauma during mastication seems likely to be involved in the pathogenesis of ulcers elsewhere in the oral cavity. PMID:2338087

Bergmann, O J; Mogensen, S C; Ellegaard, J

1990-03-01

358

Evaluation of the monocyte counting by two automated haematology analysers compared with flow cytometry.  

PubMed

The aim is to determine the monocyte count performance of the Bayer Diagnostics ADVIA120 and Coulter LH 750 automated haematology analysers and the results obtained by these two instruments were compared with those provided by Becton Dickinson FACScan flow cytometer using the combination of CD45/CD14 MoAb. Linearity and imprecision were also evaluated. The linearity of both instruments was good. Coulter LH 750 showed better precision (4.3%) than ADVIA 120 (9.0%) both within and between batch. A significant correlation (r = 0.973) was found between the LH 750 and the flow cytometry method, while a modest one was observed between the latter and the ADVIA 120 (r = 0.880). When comparing the percentage of monocytes by means of one-way anova and Tukey test, it was found that the LH 750 provided the closest results in comparison with flow cytometry, with no statistical difference between the means (mean difference MO% = 0.6); however the difference was statistically different between the ADVIA 120 and flow cytometry (mean difference MO% = -4.06). These data were confirmed by Altman-Bland and Deming regression analyses. PMID:15784123

Grimaldi, E; Carandente, P; Scopacasa, F; Romano, M F; Pellegrino, M; Bisogni, R; De Caterina, M

2005-04-01

359

Basic haematological values in antelopes--II. The Hippotraginae and the Tragelaphinae.  

PubMed

Basic haematological values in 49 animals of five species of the subfamily Hippotraginae, namely the roan antelope Hippotragus equinus, sable antelope Hippotragus niger, adax antelope Addax nasomaculatus, gemsbok oryx Oryx gazella gazella and scimitar horned oryx Oryx damah and in 51 individuals of five species in the subfamily Tragelaphinae, including the bushbuck Tragelaphus scriptus, nyala Tragelaphus angasi, greater kudu Tragelaphus strepsiceros, cape eland Tautotragus oryx and bongo Bocercus euryceros are reported. The erythrocyte counts were in the range of 12.62 X 10(12)/l in the gemsbok oryx to 6.44 X 10(12)/l in the bongo, the haematocrit values varied from 0.488 in the nyala to 0.380 in the roan antelope and for the haemoglobin highest levels (164.0 g/l) were noted in the adax antelope, and the lowest (105.5 g/l) in the bongo. Leukocyte counts were found mostly in the normal human range and varied from 7.17 X 10(9)/l in the adax antelope to 4.05 X 10(9)/l in the nyala, only in the greater kudu decreased values of 3.02 X 10(9) were estimated. These results are compared with findings taken from the literature, and with the normal human range. PMID:6149054

Pospísil, J; Kase, F; Vahala, J; Mouchová, I

1984-01-01

360

Clinical utility of blood tests in elite athletes with short term fatigue  

Microsoft Academic Search

Objective: To determine, in a population of elite athletes at their initial presentation with tiredness or fatigue, whether a set of haematological and biochemical investigations enhances the diagnostic process over and above the information gained from clinical history and examination.Methods: A sequential series of 50 elite athletes were studied at the initial consultation for a primary complaint of fatigue, tiredness,

K E Fallon

2006-01-01

361

Frequency and clinical spectrum of rare inherited coagulopathies--a tricenter study  

Microsoft Academic Search

OBJECTIVE: To determine the frequency of rare inherited coagulopathies at three centers of haematology in Karachi and to study the clinical spectrum and laboratory data of these coagulopathies.METHODS: This was a descriptive study conducted from September 2003 to December 2004 on subjects from Aga Khan University Hospital, Husaini Blood Bank and Fatimid Blood Transfusion Centre. All the subjects with bleeding

Safoorah Khalid; Fareena Bilwani; Salman Naseem Adil; Mohammad Khurshid

2008-01-01

362

Therapeutic implications of recombinant human erythropoietin in anaemic related clinical manifestations  

Microsoft Academic Search

The introduction of recombinant human erythropoietin (RHUEPO) has revolutionised the treatment strategies for patients suffering with anaemia of chronic renal disease and chronic heart failure. Clinical studies and several observational evidences have demonstrated that RHUEPO is also useful in various non-uraemic conditions including haematological and oncological disorders, prematurity, HIV infection and preoperative therapies. The successful treatment of all the anaemic

D. Annieruth Helena; A. Jyothsna; N. Lakshmi; G. Venkateswara Rao; K. R. S. Sambasiva Rao

2006-01-01

363

General Chemistry Online!  

NSDL National Science Digital Library

General Chemistry Online, created by Frederick Senese, a professor at Frostburg State University, is a guide and resource for introductory chemistry students. This site provides five areas of information: The Chemistry Home Companion, Just Ask Antoine!, Chemistry Exam Survival Guide, Tutorials, and a Featured Article. The Companion is a study guide covering the basic concepts of an introductory general chemistry course. Topics include measurement, molecules and compounds, and the quantum theory. Just Ask Antoine! allows students to post their chemistry questions. An archived list provides questions and answers for the past year. The Exam Survival Guide provides tips on passing exams, checklists of objectives and skills, and two online practice exams. The Tutorial currently contains only problems and quizzes for the measurement topic. The featured article for February is about Anandamide, a "messenger molecule that plays a role in pain, depression, appetite, memory, and fertility."

Senese, Frederick A.

364

Middle School Chemistry  

NSDL National Science Digital Library

Middle school science resource from ACS. It includes activity-based lesson plans for teaching basic chemistry concepts that cover all the main concepts in middle school chemistry. Each lesson also contains integrated animations and video that a teacher can use to help explain student observations on the molecular level. Online professional development will also be available to introduce and familiarize teachers with the demonstrations, activities, and chemistry content in the site.

365

Kurnakov and analytical chemistry  

Microsoft Academic Search

The paper appreciates the role of Academician N.S. Kurnakov in holding the All-Union Conference on Analytical Chemistry (1939)\\u000a and creating the Commission on Analytical Chemistry at the Academy of Sciences of the USSR. The scientific works of Kurnakov\\u000a relating to chemical analysis are indicated; brief review is given to studies on analytical chemistry that were carried out\\u000a in the collectives

Yu. A. Zolotov

2010-01-01

366

Science Update: Analytical Chemistry.  

ERIC Educational Resources Information Center

|Briefly discusses new instrumentation in the field of analytical chemistry. Advances in liquid chromatography, photoacoustic spectroscopy, the use of lasers, and mass spectrometry are also discussed. (CS)|

Worthy, Ward

1980-01-01

367

Environmental chemistry: Volume B  

SciTech Connect

This is an extensive introduction to environmental chemistry for engineering and chemical professionals. The contents of Volume B include equilibria processes, chemical processes, biochemical processes, and physicochemical processes.

Yen, T.F.

1999-08-01

368

Open access and medicinal chemistry  

PubMed Central

Chemistry Central is a new open access website for chemists publishing peer-reviewed research in chemistry from a range of open access journals. A new addition, Chemistry Central Journal, will cover all of chemistry and will be broken down into discipline-specific sections, and Im delighted that Medicinal Chemistry will be a key discipline in this new journal.

Swain, Chris

2007-01-01

369

21 CFR 862.3200 - Clinical toxicology calibrator.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 2013-04-01 false Clinical toxicology calibrator. 862.3200 Section 862.3200...MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3200 Clinical...

2013-04-01

370

21 CFR 862.3280 - Clinical toxicology control material.  

Code of Federal Regulations, 2013 CFR

...2013-04-01 2013-04-01 false Clinical toxicology control material. 862.3280 Section 862...MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3280 Clinical...

2013-04-01

371

21 CFR 862.3280 - Clinical toxicology control material.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Clinical toxicology control material. 862.3280 Section 862...MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3280 Clinical...

2010-04-01

372

21 CFR 862.3200 - Clinical toxicology calibrator.  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Clinical toxicology calibrator. 862.3200 Section 862.3200...MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3200 Clinical...

2010-04-01

373

21 CFR 862.3280 - Clinical toxicology control material.  

Code of Federal Regulations, 2010 CFR

...2009-04-01 2009-04-01 false Clinical toxicology control material. 862.3280 Section 862...MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3280 Clinical...

2009-04-01

374

21 CFR 862.3200 - Clinical toxicology calibrator.  

Code of Federal Regulations, 2010 CFR

...2009-04-01 2009-04-01 false Clinical toxicology calibrator. 862.3200 Section 862.3200...MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Toxicology Test Systems § 862.3200 Clinical...

2009-04-01

375

Basic Chemistry Review  

NSDL National Science Digital Library

This assignment reviews basic of chemistry for students who should have had 2 introductory semesters of basic chemistry prior to enrolling in the Fundamental of Water Quality course for which the assignment is used. Assignment reviews basic equation balancing and questions about valence and concentration conversion that students will confront regularly in any geochemistry course.

Meixner, Thomas

376

Tropospheric Halogen Chemistry  

Microsoft Academic Search

Halogens are very reactive chemicals that are known to play an important role in anthropogenic stratospheric ozone depletion chemistry, first recognized by Molina and Rowland (1974). However, they also affect the chemistry of the troposphere. They are of special interest because they are involved in many reaction cycles that can affect the oxidation power of the atmosphere indirectly by influencing

R. von Glasow; P. J. Crutzen

2003-01-01

377

WATER CHEMISTRY ASSESSMENT METHODS  

EPA Science Inventory

This section summarizes and evaluates the surfce water column chemistry assessment methods for USEPA/EMAP-SW, USGS-NAQA, USEPA-RBP, Oho EPA, and MDNR-MBSS. The basic objective of surface water column chemistry assessment is to characterize surface water quality by measuring a sui...

378

Chemistry in Microfluidic Channels  

ERIC Educational Resources Information Center

|General chemistry introduces principles such as acid-base chemistry, mixing, and precipitation that are usually demonstrated in bulk solutions. In this laboratory experiment, we describe how chemical reactions can be performed in a microfluidic channel to show advanced concepts such as laminar fluid flow and controlled precipitation. Three sets…

Chia, Matthew C.; Sweeney, Christina M.; Odom, Teri W.

2011-01-01

379

Brushing Up on Chemistry.  

ERIC Educational Resources Information Center

Presents an activity designed for use during National Chemistry Week 2002 with the theme "Chemistry Keeps Us Clean". Allows students to discover more about a cleaning product they use everyday. Students make their own toothpaste and compare its properties with those of commercial toothpaste. (MM)

Trantow, Ashley

2002-01-01

380

Infrared Lasers in Chemistry.  

ERIC Educational Resources Information Center

|Selected infrared laser chemistry topics are discussed including carbon dioxide lasers, infrared quanta and molecules, laser-induced chemistry, structural isomerization (laser purification, sensitized reactions, and dielectric breakdown), and fundamental principles of laser isotope separation, focusing on uranium isotope separation. (JN)|

John, Phillip

1982-01-01

381

Laser applications in chemistry  

SciTech Connect

This book contains the proceedings from SPIE - The International Society for Optical Engineering conference on June 5-6, 1986. Topics are presented under the following session heads: Isotope Separation and Related Photochemistry, Laser Induced Chemistry I, Laser Spectroscopy, and Laser Induced Chemistry II. There are 33 papers in this volume.

Evans, D.K.

1986-01-01

382

Rolf Claessen's Chemistry Index  

NSDL National Science Digital Library

This award is given to sites, that somehow are related to chemistry. The criteria to rank the sites are information content and design (layout, navigation, graphical design). Sites must contain chemistry related topics as innovative and attractive as possible. This award is given away to the best 5% of the submissions to the website and other sites reviewed by site author.

383

Brushing Up on Chemistry.  

ERIC Educational Resources Information Center

|Presents an activity designed for use during National Chemistry Week 2002 with the theme "Chemistry Keeps Us Clean". Allows students to discover more about a cleaning product they use everyday. Students make their own toothpaste and compare its properties with those of commercial toothpaste. (MM)|

Trantow, Ashley

2002-01-01

384

Analogies in chemistry textbooks  

Microsoft Academic Search

This study combines a critical analysis of analogies found in eight chemistry textbooks used by Australian senior high school students with the views of the textbook authors about analogies in chemistry textbooks and teaching. Sixty?two analogies were identified which described abstract chemical concepts such as atomic structure and collision theory. A range of styles, including pictorial analogies, were identified and

Rodney B. Thiele; David F. Treagust

1995-01-01

385

Chemistry and Biology  

ERIC Educational Resources Information Center

|Discusses the relationship between chemisty and biology in the science curriculum. Points out the differences in perception of the disciplines, which the physical scientists favoring reductionism. Suggests that biology departments offer a special course for chemistry students, just as the chemistry departments have done for biology students.…

Wigston, David L.

1970-01-01

386

Chemistry of americium  

Microsoft Academic Search

Essential features of the descriptive chemistry of americium are reviewed. Chapter titles are: discovery, atomic and nuclear properties, collateral reading, production and uses, chemistry in aqueous solution, metal, alloys, and compounds, and, recovery, separation, purification. Author and subject indexes are included. (JCB)

Schulz

1976-01-01

387

Chlorine Chemistry Division  

NSDL National Science Digital Library

This site is provided by the American Chemistry Council's Chlorine Chemistry Division. The page contains several links that give an introduction to chlorine, its uses, and issues surrounding the chemical compound. There is also a link to the Chlorine Science Center which provides classroom activities and a chlorine compound of the month.

2008-01-02

388

High Energy Halogen Chemistry.  

National Technical Information Service (NTIS)

Contents: Nitrosilicone Chemistry; Chemistry of 2-Fluoro-2-nitropropanediol; Experimental -- Bis(2-fluoro-2,2-dinitropropyl)silanediols; Reaction of bis(2-fluoro-2, 2-dinitropropyl) silanediol with chlorotrimethylsilane, Bis(3-3-fluoro-3,3-dinitropropyl)p...

K. Baum P. T. Berkowitz D. A. Lerdal

1978-01-01

389

Analytical chemistry in the United States  

Microsoft Academic Search

With more than 20 % of the 150,000 members of the American Chemical Society identifying themselves as analytical chemists, the staggering size of the analytical establishment in the United States becomes readily apparent. Indeed, this estimate is probably much too low since many workers in the field are members of the Association of Official Analytical Chemists, the clinical chemistry groups,

G. A. Rechnitz

1983-01-01

390

PROTEIN PRECIPITATION AS A POSSIBLE IMPORTANT PITFALL IN THE CLINICAL CHEMISTRY ANALYSIS OF BLOOD SAMPLES CONTAINING MONOCLONAL IMMUNOGLOBULINS: 2 CASE REPORTS AND A REVIEW OF THE LITERATURE. PROTEIN PRECIPITATION AS A POSSIBLE IMPORTANT PITFALL IN THE CLINICAL CHEMISTRY ANALYSIS OF BLOOD SAMPLES CONTAINING MONOCLONAL IMMUNOGLOBULINS: 2 CASE REPORTS AND A REVIEW OF THE LITERATURE  

Microsoft Academic Search

Two case reports are presented, both illustrating an analytical interference caused by monoclonal im- munoglobulins. Falsely low results were obtained in the routine analysis of glucose, CRP and HDL-cho- lesterol. When analysing samples containing paraproteins, various problems can be encountered in the clinical laboratory: next to the antibody effect, pseudohy- ponatraemia, hyperviscosity, cryoglobulinaemia and gel formation have to be taken

M. Berth; J. Delanghe

391

A clinical comparison of intranasal budesonide with beclomethasone dipropionate for perennial non-allergic rhinitis: a 12 month study.  

PubMed

To evaluate possible differences in efficacy and safety between budesonide and beclomethasone dipropionate when used intranasally in the treatment of perennial non-allergic rhinitis, a 12-month open study was undertaken in 24 patients suffering from perennial non-allergic rhinitis. Both drugs were applied intranasally from pressurised aerosols at a daily dosage of 400 micrograms. On entry and at visits after 1, 2, 4, 6, 9 and 12 months, rhinoscopy was performed and the severity of nasal symptoms graded according to a four-point rating scale. All nasal symptoms were reduced from baseline during the treatment period in both groups. Tachyphylaxis was not observed. No clinically significant changes in haematology or blood chemistry parameters were observed in either group, and analysis of plasma cortisol levels revealed no influence of either drug on the hypothalamic-pituitary-adrenal axis. Local adverse reactions were uncommon and mild. Budesonide and beclomethasone dipropionate used intranasally at 400 micrograms per day were found to be safe, and budesonide was found to have a significantly higher (p < 0.05) efficacy than beclomethasone dipropionate in alleviating symptoms of perennial non-allergic rhinitis. PMID:9015907

Synnerstad, B; Lindqvist, N

392

A data management software for the Sysmex NE 8000 haematology analyser.  

PubMed

The Sysmex NE 8000 (TOA-Japan) is a haematology analyser that performs blood cells count and leukocyte differential count. For facilitating the work of technical validation, we developed a software adapted to any IBM or compatible PC running under MS-DOS, to manage the analyser. Data are automatically collected via the RS-232 interface from the analyser or keyed in for the other techniques. The software deals with 64 different analyses entirely "user defined". Six technical alarms of the analyser are taken in account for red or white cells and platelets. An "electronic worksheet" presents the results or alarms with 10 patients to a page. This enables the lab technician to assess the coherence of the various data and to perform verifications or complementary tests if necessary. As an option, a blinking asterisk can signal any results out of predetermined range. By moving the cursor through the table, a test result can be deleted, modified or added. A function displays the patient previous files in a window because the data are recorded in long term archives at the end of the day. This long term recording allows a search of previous files to decide additional tests if the patient is unknown. If the patient is known, with additional tests previously performed, this procedure is time saving. A daily archive function classifies and prints the whole day's work in alphabetical order. A protocol of communication allows a connection to a mainframe computer Bayer-Technicon. This program and the user's manual are free of charge, available on request from J. P. Cambus. PMID:8814394

Cambus, J P; Nguyen, F; Cambus, F

1996-07-01

393

Chicken antibodies: a clinical chemistry perspective.  

PubMed

The chicken immune system has been studied for many years and these studies have contributed substantially to our understanding of the fundamental concepts of immunology and the development of different immunoglobulin classes. It is thus surprising that only a small fraction of the antibodies presently used in laboratories are of avian origin. A laying hen produces more yolk antibodies than a rabbit can produce during the same time period, and the animal care costs are lower for the chicken compared to the rabbit. Chicken antibodies offer many advantages to the traditional mammalian antibodies when used for the detection of mammalian antigen. Due to the evolutionary difference chicken IgY will react with more epitopes on a mammalian antigen, which will give an amplification of the signal. Chicken antibodies can also be used to avoid interference in immunological assays caused by the human complement system, rheumatoid factors, human anti-mouse IgG antibodies (HAMA) or human and bacterial Fc-receptors. The antibodies can be purified in large amounts from egg yolk, making laying hens highly efficient producers of polyclonal antibodies. PMID:10680951

Carlander, D; Stålberg, J; Larsson, A

1999-01-01

394

IND Applications for Clinical Investigations: Chemistry ...  

Center for Drug Evaluation (CDER)

... Note: For placebo, the Quality Control test will include the absence of the active pharmaceutical ingredient(s). The physical characteristics of the ... More results from www.fda.gov/drugs/developmentapprovalprocess/howdrugsaredevelopedandapproved

395

Korean Kimchi Chemistry: A Multicultural Chemistry Connection  

ERIC Educational Resources Information Center

|Connecting science with different cultures is one way to interest students in science, to relate science to their lives, and at the same time to broaden their horizons in a variety of ways. In the lesson described here, students make kimchi, a delicious and popular Korean dish that can be used to explore many important chemistry concepts,…

Murfin, Brian

2009-01-01

396

EVOLVING FROM GREEN CHEMISTRY TO SUSTAINABLE CHEMISTRY  

EPA Science Inventory

The twelve principles of green chemistry provide a foundation and pathway which allows researchers to incorporate greenness into existing reactions or when developing new technologies. Research from our laboratory has adopted many of these principles and utlizes them as a major c...

397

Korean Kimchi Chemistry: A Multicultural Chemistry Connection  

ERIC Educational Resources Information Center

Connecting science with different cultures is one way to interest students in science, to relate science to their lives, and at the same time to broaden their horizons in a variety of ways. In the lesson described here, students make kimchi, a delicious and popular Korean dish that can be used to explore many important chemistry concepts,…

Murfin, Brian

2009-01-01

398

Some changes in the haematological parameters of rainbow trout ( Oncorhynchus mykiss ) exposed to three artificial photoperiod regimes  

Microsoft Academic Search

Haematological parameters are often used as health status and stress indicators in fish. However, information on the effects\\u000a of artificial photoperiods on these parameters is scarce and ambiguous. The consequences of three different artificial photoperiod\\u000a regimes [light\\/day 12 h:12 h (LD 12:12) for 150 days as controls; LD 14:10 and LD 24:0, both for an initial 60 days, and then\\u000a LD 12:12 for the remaining 90 days] were evaluated

A. E. Valenzuela; V. M. Silva; A. E. Klempau

2007-01-01

399

Moderator Chemistry Program  

SciTech Connect

Over the past fifteen months, the Systems Chemistry Group of the Reactor Engineering Department has undertaken a comprehensive study of the Department's moderator chemistry program at Savannah River Site (SRS). An internal review was developed to formalize and document this program. Objectives were as outlined in a mission statement and action plan. In addition to the mission statement and action plan, nine separate task reports have been issued during the course of this study. Each of these task reports is included in this document as a chapter. This document is an organized compilation of the individual reports issued by the Systems Chemistry Group in assessment of SRS moderator chemistry to determine if there were significant gaps in the program as ft existed in October, 1989. While these reviews found no significant gaps in that mode of operation, or any items that adversely affected safety, items were identified that could be improved. Many of the items have already been dear with or are in the process of completion under this Moderator Chemistry Program and other Reactor Restart programs. A complete list of the items of improvement found under this assessment is found in Chapter 9, along with a proposed time table for correcting remaining items that can be improved for the chemistry program of SRS reactors. An additional external review of the moderator chemistry processes, recommendations, and responses to/from the Reactor Corrosion Mitigation Committee is included as Appendix to this compilation.

Dewitt, L.V.; Gibbs, A.; Lambert, D.P.; Bohrer, S.R.; Fanning, R.L.; Houston, M.W.; Stinson, S.L.; Deible, R.W.; Abdel-Khalik, S.I.

1990-11-01

400

Moderator Chemistry Program  

SciTech Connect

Over the past fifteen months, the Systems Chemistry Group of the Reactor Engineering Department has undertaken a comprehensive study of the Department`s moderator chemistry program at Savannah River Site (SRS). An internal review was developed to formalize and document this program. Objectives were as outlined in a mission statement and action plan. In addition to the mission statement and action plan, nine separate task reports have been issued during the course of this study. Each of these task reports is included in this document as a chapter. This document is an organized compilation of the individual reports issued by the Systems Chemistry Group in assessment of SRS moderator chemistry to determine if there were significant gaps in the program as ft existed in October, 1989. While these reviews found no significant gaps in that mode of operation, or any items that adversely affected safety, items were identified that could be improved. Many of the items have already been dear with or are in the process of completion under this Moderator Chemistry Program and other Reactor Restart programs. A complete list of the items of improvement found under this assessment is found in Chapter 9, along with a proposed time table for correcting remaining items that can be improved for the chemistry program of SRS reactors. An additional external review of the moderator chemistry processes, recommendations, and responses to/from the Reactor Corrosion Mitigation Committee is included as Appendix to this compilation.

Dewitt, L.V.; Gibbs, A.; Lambert, D.P.; Bohrer, S.R.; Fanning, R.L.; Houston, M.W.; Stinson, S.L.; Deible, R.W.; Abdel-Khalik, S.I.

1990-11-01

401

Clinical differentiation between dogs with benign and malignant spirocercosis  

Microsoft Academic Search

Spirocerca lupi is a nematode infesting the canine oesophagus, where it induces the formation of a nodule that may transform into a malignant sarcoma. The current, retrospective study compared the clinical presentation, haematology, serum albumin and globulin and radiology of benign cases (n=31) and malignant cases (n=31) of spirocercosis.Dogs with spirocercosis-induced sarcoma were significantly older (6.4±1.91 years) than benign cases

Eran Dvir; Robert M. Kirberger; Varaidzo Mukorera; Liesel L. van der Merwe; Sarah J. Clift

2008-01-01

402

The Chemistry Resource Center  

NSDL National Science Digital Library

Maintained by the British Columbia Institute of Technology Chemistry Department, The Chemistry Resource Center Web site bills itself as "on-line multi-media tutor to direct students to find textbook references, interesting WWW links and to guide them to solve Chemistry problems." Among the many activities on the site, probably the most interesting is the "How well do you know your Periodic Table?" link, which helps to teach students the basics of the elements and the periodic table through a unique interactive online exercise.

2000-01-01

403

Green Chemistry Teaching Resources  

NSDL National Science Digital Library

The American Chemical Society (ACS) has created these excellent resources via the Green Chemistry Institute and the ACS Education Division. The goal of these materials is "to increase awareness and understanding of Green Chemistry principles, alternatives, practices, and benefits within traditional educational institutions and among practicing scientists." In the Online Resources section, visitors will find downloadable pocket guides to basic green chemistry principles, "Greener Education Materials for Chemists" from the University of Oregon, and more. Perhaps the best section of the site is Activities and Experiments, where visitors can look over activities like "Gassing Up Without Air Pollution" and "Cleaning Up With Atom Economy."

2012-10-26

404

Green Chemistry Teaching Resources  

NSDL National Science Digital Library

The American Chemical Society (ACS) has created these excellent resources via the Green Chemistry Institute and the ACS Education Division. The goal of these materials is "to increase awareness and understanding of Green Chemistry principles, alternatives, practices, and benefits within traditional educational institutions and among practicing scientists." In the Online Resources section, visitors will find downloadable pocket guides to basic green chemistry principles, "Greener Education Materials for Chemists" from the University of Oregon, and more. Perhaps the best section of the site is Activities and Experiments, where visitors can look over activities like "Gassing Up Without Air Pollution" and "Cleaning Up With Atom Economy."

405

Wizardry and Chemistry  

NSDL National Science Digital Library

Students learn how common pop culture references (Harry Potter books) can relate to chemistry. While making and demonstrating their own low-intensity sparklers (muggle-versions of magic wands), students learn and come to appreciate the chemistry involved (reaction rates, Gibb's free energy, process chemistry and metallurgy). The fun part is that all wands are personalized and depend on how well students conduct the lab. Students end the activity with a class duelâa face-off between wands of two different chemical compositions. This lab serves as a fun, engaging review for stoichiometry, thermodynamics, redox and kinetics, as well as advanced placement course review.

University Of Houston

406

Microscale Gas Chemistry  

NSDL National Science Digital Library

Created by Professor Bruce M. Mattson, PhD, of Creighton University's Department of Chemistry, the Microscale Gas Chemistry Website "provides instructions for the generation of gases on a microscale level along with instructions for chemical demonstrations and student laboratory experiments with the gases." The no-frills site, designed for high school and university chemistry teachers, contains clear and careful instructions for experiments with carbon dioxide, hydrogen, oxygen and nitrogen, nitrogen oxides, ammonia, ethene, chlorine, carbon monoxide, and more. An introduction offers two low-tech methods for gas generation. Data pages for relevant gases are linked to each experiment.

407

Materials Chemistry at SFU  

NSDL National Science Digital Library

The Materials Science group at Simon Fraser University (SFU) developed this website to address the group's primary research interests in material synthesis, molecular, electronics, and photonics. Visitors will find explanations covering sixteen research topics including chemical sensors, lithography, non-linear optics, and supramolecular chemistry. Under each topic heading, users will find links discussing the faculties' current goals, recent publications, and patents. The site also features links to the Pacific Centre for Advanced Materials and Microstructures; a collaborative effort between the Materials Science group at SFU and the physics and chemistry departments at the University of British Columbia. Anyone searching for the latest investigations in materials chemistry will find this website very informative.

408

Retrospective study of the epidemiological, clinical, haematological and biochemical findings in 109 dogs poisoned by Vipera xanthina palestinae  

Microsoft Academic Search

One hundred and nine dogs were diagnosed as having been poisoned by viper (Vipera xanthina palestinae) venom between 1989 and 1996. Most of the cases occurred between April and September (86.2 per cent), with peaks in May (25.7 per cent) and July (20.2 per cent), and very few between November and February (3.6 per cent). Forty-two per cent of the

I. Aroch; S. Harrus

1999-01-01

409

Comparative Haematological Screening of Urban and Rural Pregnant Women Attending Antenatal Clinics in Lagos and Its Environs.  

ERIC Educational Resources Information Center

|Compared blood screening data for 200 urban and rural pregnant women in Nigeria. Found that rural subjects had a greater incidence of moderate anemia than did urban subjects, and corpuscular hemoglobin concentrations fell with increased gestational age. No relationship was found between hemoglobin counts and nutrition habits. (HTH)|

Abidoye, R. O.; Olukoya, A. A.

1993-01-01

410

Mucosal barrier injury: biology, pathology, clinical counterparts and consequences of intensive treatment for haematological malignancy: an overview  

Microsoft Academic Search

Mucositis is an inevitable side-effect of the conditioning regimens used for haematopoietic stem cell transplantation. The condition is better referred to as mucosal barrier injury (MBI) since it is primarily the result of toxicity and is a complex and dynamic pathobiological process manifested not only in the mouth but also throughout the entire digestive tract. A model has been proposed

NMA Blijlevens; JP Donnelly; BE De Pauw

2000-01-01

411

Sequencing General Chemistry  

NSDL National Science Digital Library

The material in the authors' general chemistry curriculum has been rearranged into a sequence thought to be more logical to students than the traditional sequence. This fresh approach does not radically change course content but rather produces a systemat

Yoblinski, B. J.

2003-03-01

412

Chemistry, Population, Resources.  

National Technical Information Service (NTIS)

Chemistry plays a central role in the global development of the world's human population and their resources. Its effect is exercised primarily through (1) the production of food, (2) public health, and (3) medical practice and drugs. These factors togeth...

M. Calvin

1977-01-01

413

Chemistry with a Peel.  

ERIC Educational Resources Information Center

Presents experiments that introduce natural product chemistry into high school classrooms. In the laboratory activities, students isolate and analyze the oil in orange peels. Students also perform a steam distillation and learn about terpenes. (DDR)

Borer, Londa; Larsen, Eric

1997-01-01

414

Virtual Chemistry Experiments  

NSDL National Science Digital Library

Chemistry applets simulate various experiments, apparatus, and chemical processes. The display and operation of an applet can be controlled by the user through the PARAM options in the APPLET tag and through JavaScript commands.

Blauch, David N.

415

Chemistry with a Peel.  

ERIC Educational Resources Information Center

|Presents experiments that introduce natural product chemistry into high school classrooms. In the laboratory activities, students isolate and analyze the oil in orange peels. Students also perform a steam distillation and learn about terpenes. (DDR)|

Borer, Londa; Larsen, Eric

1997-01-01

416

Chemistry Laboratory Safety Check  

ERIC Educational Resources Information Center

|An accident prevention/safety check list for chemistry laboratories is printed. Included are checks of equipment, facilities, storage and handling of chemicals, laboratory procedures, instruction procedures, and items to be excluded from chemical laboratories. (SL)|

Patnoe, Richard L.

1976-01-01

417

Opportunities in Chemistry.  

National Technical Information Service (NTIS)

Chemistry is a central science that responds to societal needs. It is critical in man's attempts to discover new processes, tap new sources of energy, develop new materials, feed the world's population, improve health and conquer disease, monitor and prot...

1985-01-01

418

Wet Chemistry Instrument Prototype.  

National Technical Information Service (NTIS)

A wet chemistry instrument prototype for detecting amino acids in planetary soil samples was developed. The importance of amino acids and their condensation products to the development of life forms is explained. The characteristics of the instrument and ...

1974-01-01

419

General Chemistry Multimedia Problems  

NSDL National Science Digital Library

General Chemistry Multimedia Problems ask students questions about experiments they see presented using videos and images. The questions asked apply concepts from different parts of an introductory course, encouraging students to decompartmentalize the material.

420

Chemistry and Detective Fiction.  

ERIC Educational Resources Information Center

Describes an interdisciplinary program consisting of two courses. The first course deals with the chemistry of drugs and poisons; the second course focuses on fictional works in which these drugs and poisons are central to the plots. (SK)

Labianca, Dominick A.; Reeves, William J.

1981-01-01

421

Organic Chemistry Forum  

NSDL National Science Digital Library

ChemWeb's Organic Chemistry Forum bills itself as a "free online club for the organic chemistry community." Users will need to complete a simple online form to obtain membership and access the site. One highlight of this site is free full-text access to Tetrahedron Letters. This rapid publication journal appears weekly. Along with free access to Beilstein Abstracts, the site includes jobs, a conference diary, news, discussion groups, and more.

2005-01-01

422

UCLA: Organic Chemistry Tutorials  

NSDL National Science Digital Library

Steven Hardinger at the Department of Chemistry and Biochemistry at UCLA created these tutorials to assist students with the difficult concepts presented in introductory organic chemistry. Students can find tutorials dealing with acids and bases, carbocations, Lewis dot structures, and more. Within each tutorial, users can find links to a dictionary that adequately explain the unfamiliar terminology. The tutorials include example problems and exercises to challenge users.

Hardinger, Steven

423

Nicotine Smoke Chemistry  

NSDL National Science Digital Library

The featured molecules this month come from the paper Using "Basic Principles" To Understand Complex Science: Nicotine Smoke Chemistry and Literature Analogies by Jeffrey Seeman detailing some of the complexities involved in the volatilization of two alkaloids, nicotine and cocaine. Students could be asked to identify how chemistry is involved in the various steps described in the paper, and most beginning students will be surprised to learn just how complex a process the volatilization of a molecule such as nicotine is.

424

The chemistry of krypton  

Microsoft Academic Search

Krypton is the only noble gas other than xenon to give rise to isolable compounds in macroscopic amounts, although the chemistry of krypton is presently limited to the +2 oxidation state. The strong oxidant-fluorinator properties and thermal instabilities of krypton(II) compounds have posed considerable challenges to determining the extent to which the chemistries of krypton(II) and xenon(II) are analogous. Krypton(II)

John F. Lehmann; Hélène P. A. Mercier; Gary J. Schrobilgen

2002-01-01

425

General Chemistry I  

NSDL National Science Digital Library

From Dr. Michael Bleber of Florida State University's Department of Chemistry, this page contains a virtual guide for a first semester undergraduate course in general chemistry. The site features lecture notes, practice homework, practice exams, homework assignments, useful tables, and a FAQ. The variety and depth of materials at this site make it useful for educators looking for teaching aids, as well as students in search of additional course information.

426

Darwinian evolution and chemistry  

Microsoft Academic Search

Some significant results are collected with a view to ascertain whether a Darwinian chemical evolution is at work in chemistry\\u000a and, if so, to define its specific characters. To this aim prebiotic chemistry, homochirality; chemical reactions and emergence\\u000a of new properties are considered. The Darwinian process of mutation by chance, selection and replication appears to translate\\u000a into chemical exploration of

Gian Paolo Chiusoli

2009-01-01

427

Biochemical and haematological evaluation of repeated dose exposure of male Wistar rats to an ethanolic extract of Artemisia annua.  

PubMed

Artemisia annua is widely used for the treatment of malaria and other disorders. In a previous study, the artemisinin concentration in the dry leaves of A. annua grown under humid tropical conditions was determined to be 1.098% using reversed phase high performance liquid chromatography. In the current study, biochemical and haematological evaluations of ethanolic leaf extracts derived from such plants (EAA) were carried out in 20 male Wistar rats. Rats were divided into four study groups of saline-treated (control) and test groups exposed orally to graded doses of EAA for 28 days. The results showed that the liver function and haematological indices, and testosterone levels were not adversely affected. High density lipoprotein -cholesterol was reduced at 100?mg/kg of EAA, atherogenic index as well as low density lipoprotein -cholesterol was raised, and glucose concentration was reduced significantly at the 100 and 200?mg/kg of EAA (p?

Eteng, Mbeh U; Abolaji, Amos O; Ebong, Patrick E; Brisibe, Ebiamadon Andi; Dar, Ahsana; Kabir, Nurul; Iqbal Choudhary, M

2012-06-27

428

Comparison of the performance, carcass characteristics and haematological parameters of broiler chicks reared in cage and floor.  

PubMed

This study was conducted to determine the effect of housing systems on the performance, carcass characteristics and the haematology of 150 four-week old Anak titan strains of broiler chicks in a completely randomized design. The birds were allocated randomly into two treatment (cage and floor) groups of three replications containing 25 birds per replicate. They were maintained for a period of 4 weeks and data were collected on; final body weight, weight gain, feed intake, feed:gain and mortality. At the 56th week, carcass yield, cut-up parts and organs weights as well as some parameters were measured. The results showed that feed intake and feed:gain were significantly (p < 0.05) higher in the cage system. Dressing percentage and breast weight were higher (p < 0.05) in the floor housing system while drumstick and lung weight were higher in the cage system. No significant (p > 0.05) difference was obtained in the haematology. It was then concluded that an improvement in the floor system could result into a better performance and an increased carcass yield. PMID:18817178

Sogunle, O M; Egbeyale, L T; Bajomo, T T; Bamigboje, O V; Fanimo, A O

2008-02-01

429

Protective effects of vitamin C against haematological and biochemical toxicity induced by deltamethrin in male Wistar rats.  

PubMed

Deltamethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations. The present experiment pertains to the protective role of vitamin C against haematological and biochemical toxicity induced by deltamethrin during 4 weeks. Male Wistar rats were divided into four groups of eight each: Group I served as control rats; Group II received deltamethrin (1.28 mg/kg BW) in drinking water. Group III received both deltamethrin and vitamin C (200mg/kg BW; by i.p. injection); Group IV received vitamin C (200mg/kg BW). Exposure of rats to deltamethrin caused significant changes of some haematological parameters (red blood cells (RBC), haemoglobin (Hb), haematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet (Plt) and white blood cells (WBC)) in treated rats compared to controls. Significant increases in the levels of hepatic markers enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), ?-Glutamyl transpeptidase (?-GT)). Furthermore, renal markers such as urea and creatinine were increased in deltamethrin treated rats. Additionally, serum cholesterol and lipid peroxidation were significantly enhanced. Co-administration of vitamin C to the group III restored all the parameters cited above to near-normal values. Therefore, our investigation revealed that vitamin C appeared to be a promising agent for protection against deltamethrin-induced toxicity. PMID:21514672

Mongi, Saoudi; Mahfoud, Messarah; Amel, Boumendjel; Kamel, Jamoussi; Abdelfattah, El Feki

2011-04-22

430

Genetic analysis of haematological and plasma biochemical parameters in the Spanish purebred horse exercised on a treadmill.  

PubMed

The novel aim of this study was to describe the reference values of different haematological and biochemical parameters in the Spanish purebred horse (Andalusian, SPB) in each of the stages of a programmed exercise on a treadmill system, and to establish heritability and genetic correlations for these haematological and biochemical parameters. For this, 94 young SPB male horses (4.22 ± 2.27 years old) were used. An increasing intensity exercise test at 4, 5, 6 and 7 m/s was carried out on a treadmill (6% inclination). Total red blood cells, total white blood cells, neutrophils and lymphocytes counts; haematocrit, haemoglobin, lactate, uric acid, creatinine and total plasma proteins concentrations and aspartate transaminase, lactate dehydrogenase, creatine-quinase activities were determined. To conclude: (i) the reference values for each parameter were determined for each of the exercise test stages (ii) all the parameters analysed manifested a medium-high heritability and a high repeatability. These results will, in the near future, determine the measuring guidelines for improving the SPB horse's athletic ability on an objective treadmill system and for selecting these animals in response to those parameters. PMID:23702348

Escribano, B M; Molina, A; Valera, M; Tovar, P; Agüera, E I; Santisteban, R; Vivo, R; Agüera, S; Rubio, M D

2013-05-24

431

Haematological profile of crossbred dairy cattle to monitor herd health status at medium elevation in Central Himalayas.  

PubMed

Haematological profile-haemoglobin concentration (Hb), total erythrocytes count (TEC), packed cell volume (PCV), erythrocyte indices-mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were studied in crossbred dairy cattle (Holstein Friesian x Sahiwal) under various physiological states: non-pregnant heifers (NPH), pregnant heifers (PH), empty dry cows (EDC), pregnant lactating cows (PLC), medium yield early lactating cows (MYELC) and high yield early lactating cows (HYELC) during summer and winter seasons at 1700 metres altitude from mean sea level in the Central Himalayas. On comparison of annual means, the highest values of Hb and PCV were recorded in PH and of TEC in NPH, whereas the lowest values of these parameters were found in EDC. The Hb and TEC tended to decrease with increasing milk yield. Comparison of annual means of erythrocyte indices revealed the highest MCV and MCH in EDC, which simultaneously showed the lowest MCHC. Significant seasonal variations in haematological profile were recorded. The overall group mean (OGM) of Hb, MCV, MCH and MCHC was found to be significantly higher (P < 0.01) during summer whereas the TEC and PCV showed higher OGM (P < 0.01) during the winter season. PMID:11020365

Kumar, B; Pachauri, S P

2000-10-01

432

21 CFR 862.2860 - Mass spectrometer for clinical use.  

Code of Federal Regulations, 2010 CFR

...AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Laboratory Instruments...spectrometer for clinical use is a device intended to identify inorganic or organic compounds (e.g., lead, mercury,...

2009-04-01

433

21 CFR 862.2860 - Mass spectrometer for clinical use.  

Code of Federal Regulations, 2010 CFR

...AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Laboratory Instruments...spectrometer for clinical use is a device intended to identify inorganic or organic compounds (e.g., lead, mercury,...

2010-04-01

434

21 CFR 862.2680 - Microtitrator for clinical use.  

Code of Federal Regulations, 2013 CFR

...DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Laboratory Instruments § 862.2680 Microtitrator for clinical use. (a) Identification....

2013-04-01

435

21 CFR 862.2700 - Nephelometer for clinical use.  

Code of Federal Regulations, 2013 CFR

...DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Laboratory Instruments § 862.2700 Nephelometer for clinical use. (a)...

2013-04-01

436

Dynamic covalent chemistry.  

PubMed

Dynamic covalent chemistry relates to chemical reactions carried out reversibly under conditions of equilibrium control. The reversible nature of the reactions introduces the prospects of "error checking" and "proof-reading" into synthetic processes where dynamic covalent chemistry operates. Since the formation of products occurs under thermodynamic control, product distributions depend only on the relative stabilities of the final products. In kinetically controlled reactions, however, it is the free energy differences between the transition states leading to the products that determines their relative proportions. Supramolecular chemistry has had a huge impact on synthesis at two levels: one is noncovalent synthesis, or strict self-assembly, and the other is supramolecular assistance to molecular synthesis, also referred to as self-assembly followed by covalent modification. Noncovalent synthesis has given us access to finite supermolecules and infinite supramolecular arrays. Supramolecular assistance to covalent synthesis has been exploited in the construction of more-complex systems, such as interlocked molecular compounds (for example, catenanes and rotaxanes) as well as container molecules (molecular capsules). The appealing prospect of also synthesizing these types of compounds with complex molecular architectures using reversible covalent bond forming chemistry has led to the development of dynamic covalent chemistry. Historically, dynamic covalent chemistry has played a central role in the development of conformational analysis by opening up the possibility to be able to equilibrate configurational isomers, sometimes with base (for example, esters) and sometimes with acid (for example, acetals). These stereochemical "balancing acts" revealed another major advantage that dynamic covalent chemistry offers the chemist, which is not so easily accessible in the kinetically controlled regime: the ability to re-adjust the product distribution of a reaction, even once the initial products have been formed, by changing the reaction's environment (for example, concentration, temperature, presence or absence of a template). This highly transparent, yet tremendously subtle, characteristic of dynamic covalent chemistry has led to key discoveries in polymer chemistry. In this review, some recent examples where dynamic covalent chemistry has been demonstrated are shown to emphasise the basic concepts of this area of science. PMID:12491278

Rowan, Stuart J; Cantrill, Stuart J; Cousins, Graham R L; Sanders, Jeremy K M; Stoddart, J Fraser

2002-03-15

437

Robenacoxib vs. carprofen for the treatment of canine osteoarthritis; a randomized, noninferiority clinical trial.  

PubMed

Robenacoxib is a member of the coxib class of nonsteroidal anti-inflammatory drugs (NSAID), with high selectivity for the cyclooxygenase (COX)-2 isoform of COX. In this study, the efficacy and tolerability of robenacoxib were compared with those of carprofen in canine osteoarthritis in a multi-centre, prospective, randomized, blinded, positive-controlled noninferiority clinical trial. Both drugs were administered orally once daily at recommended dosages: robenacoxib at 1-2 mg/kg (n = 125 dogs) and racemic carprofen at 2-4 mg/kg (n = 63 dogs) for a total of 12 weeks. The efficacy of the test compounds was assessed by veterinary investigators and owners using numerical rating scales at baseline and days 7, 14, 28, 56 and 84. In both groups, all scores were significantly (P < 0.0001) improved compared with baseline at all time points (days 7-84). Robenacoxib had noninferior efficacy to carprofen for the primary endpoint, the global functional disability, both for all dogs and for the subgroup of dogs in which robenacoxib was not administered during meals. Noninferiority was also demonstrated for three of six veterinary investigator secondary endpoints and four of six owner efficacy endpoints. For haematology and clinical chemistry variables, there were some significant differences from baseline levels but no differences between groups. There were no differences between groups in the frequencies of adverse events, which were reported in 46% dogs with robenacoxib and 52% with carprofen (P = 0.44), which were most frequently mild events affecting the gastrointestinal tract. In conclusion, noninferior efficacy and tolerability of robenacoxib compared with carprofen was demonstrated in dogs with osteoarthritis. PMID:21480932

Reymond, N; Speranza, C; Gruet, P; Seewald, W; King, J N

2011-04-12

438

April 25, 2013: Clinical Chemistry and Clinical Toxicology ...  

Center for Biologics Evaluation and Research (CBER)

... make recommendations on the appropriate regulatory classification for diagnostic devices known as phencyclidine (PCP) enzyme immunoassays ... More results from www.fda.gov/advisorycommittees/calendar

439

April 25-26, 2013: Clinical Chemistry and Clinical Toxicology ...  

Center for Biologics Evaluation and Research (CBER)

... committee will discuss and make recommendations on the appropriate regulatory classification for diagnostic devices known as isoniazid test strips ... More results from www.fda.gov/advisorycommittees/calendar

440

Effect of Ramadan fasting on some biochemical and haematological parameters in Tunisian youth soccer players undertaking their usual training and competition schedule  

Microsoft Academic Search

Competitive Muslim athletes often have to train and compete during the holy month of Ramadan when they abstain from food and drink from sunrise to sunset. In this study, we investigated the effect of Ramadan fasting on some biochemical and haematological parameters in 78 Tunisian junior male soccer players aged 16–19 years who continued their usual schedule of daily training

Ronald J. Maughan; John B. Leiper; Zakia Bartagi; Rym Zrifi; Yacine Zerguini; Jiri Dvorak

2008-01-01

441

Effects of prolonged strenuous exercise (marathon running) on biochemical and haematological markers used in the investigation of patients in the emergency department  

Microsoft Academic Search

Objectives: To investigate the effects of strenuous exercise on commonly used biochemical and haematological variables in subjects running the 2002 London marathon.Methods: 34 healthy volunteers (7 female, 27 male) were recruited for the study. Blood was taken before the start (at registration) and immediately after completion of the marathon. Samples were analysed for urea and electrolytes, liver function tests, creatine

J E Smith; G Garbutt; P Lopes; D Tunstall Pedoe

2004-01-01

442

Evaluation of cinnamon and garlic as antibiotic growth promoter substitutions on performance, immune responses, serum biochemical and haematological parameters in broiler chicks  

Microsoft Academic Search

This study was performed to investigate the effect of dietary supplementation with cinnamon and garlic powder as growth promoter agents on performance, carcase traits, immune responses, serum biochemistry, haematological parameters and thigh meat sensory evaluation in broilers. A total of 288, day-old male broilers (Ross 308) were randomly assigned to six treatments with four replicates. The dietary treatments consisted of

Mehdi Toghyani; Majid Toghyani; Abbasali Gheisari; Gholamreza Ghalamkari; Shahin Eghbalsaied

2011-01-01

443

Responses to submaximal treadmill exercise and training in the horse: changes in haematology, arterial blood gas and acid base measurements, plasma biochemical values and heart rate  

Microsoft Academic Search

Four standardbred horses with subcutaneously relocated carotid arteries were given a seven week training programme of treadmill exercise at a gradient of 19 per cent in order to assess if there were any effects of exercise and training on haematology, arterial blood gas and acid base measurements, plasma biochemistry and heart rate. The exercise consisted of one minute walking at

RJ Rose; JR Allen; JH Stewart; W Chan

1983-01-01

444

Technetium Chemistry in HLW  

SciTech Connect

Tc contamination is found within the DOE complex at those sites whose mission involved extraction of plutonium from irradiated uranium fuel or isotopic enrichment of uranium. At the Hanford Site, chemical separations and extraction processes generated large amounts of high level and transuranic wastes that are currently stored in underground tanks. The waste from these extraction processes is currently stored in underground High Level Waste (HLW) tanks. However, the chemistry of the HLW in any given tank is greatly complicated by repeated efforts to reduce volume and recover isotopes. These processes ultimately resulted in mixing of waste streams from different processes. As a result, the chemistry and the fate of Tc in HLW tanks are not well understood. This lack of understanding has been made evident in the failed efforts to leach Tc from sludge and to remove Tc from supernatants prior to immobilization. Although recent interest in Tc chemistry has shifted from pretreatment chemistry to waste residuals, both needs are served by a fundamental understanding of Tc chemistry.

Hess, Nancy J.; Felmy, Andrew R.; Rosso, Kevin M.; Xia Yuanxian

2005-06-06

445

Residue chemistry guidelines.  

PubMed

Residue chemistry guidelines are designed to determine what the potential residues in food are and how much may be present as a result of pesticide application, so that a tolerance level may be established. Some requirements are established to assist in the enforcement of tolerances by the USDA, FDA, and the states. I realize I have given you a quick overview of the residue chemistry requirements. There are many documents which are available if you should require more information, such as the Subdivision O Residue Chemistry Guidelines, Standard Evaluation Procedures (which are used by reviewers when evaluating the studies), the Data Reporting Guidelines (which provide guidance on preparing final reports), and the Technical Guidance from Phase III of Reregistration. We have also released various papers on studies when additional guidance is required. Most of these documents are available from NTIS. I hope you will consider this information when auditing residue chemistry studies. As I see the efforts that you, the QA professionals, have made to educate yourselves on residue chemistry studies through programs such as this meeting, I have a little more confidence in answering the question "Do you trust them?" with a "Yes." Thank you. PMID:8156199

Olinger, C L; Schmitt, R D; Zager, E

446

Clinical and cytogenetic diversity in Fanconi's anaemia.  

PubMed Central

Abnormally high levels of spontaneous and mitomycin C or diepoxybutane induced chromosome breakage were observed in lymphocytes from eight out of nine previously undescribed patients clinically diagnosed as having Fanconi's anaemia. The results suggest that the combination of spontaneous and induced chromosome breakage is a good aid in the differential diagnosis and we suggest that increased chromosome breakage is pathognomonic for this recessive disorder. It is, however, not possible to demonstrate consistently raised levels of induced chromosome breakage in obligate carriers. The patient who had normal levels of chromosome breakage had an atypical haematological picture and may suffer from a disease genetically different from Fanconi's anaemia.

Duckworth-Rysiecki, G; Hulten, M; Mann, J; Taylor, A M

1984-01-01

447

Haematological and serum biochemical parameters of West African Dwarf goats fed dried cassava leaves-based concentrate diets.  

PubMed

The study was conducted to investigate the effects of feeding different levels of dried cassava leaves at 0%, 20%, 40% and 60%, respectively, using guinea grass as basal feed, on the haematological and serum biochemical parameters of West African Dwarf (WAD) goats. The study lasted for 116 days during which haematological and serum biochemical parameters were monitored in 40 male goats before and after, using a completely randomized design. At the start of the experiment, packed cell volume (PCV) ranged from 21.5% to 25.5% while haemoglobin concentration (Hb) and RBC significantly (P??0.05). Lymphocyte reduced significantly (P??0.05) at the 0% to 40% levels and reduced at the 60% level of dried cassava leaves inclusion. At the start of the experiment, values for glucose significantly (P?haematological and serum biochemical parameters of WAD goats and could therefore be included in ruminant diets up to 60%. PMID:21744028

Oni, Adebayo Olusoji; Arigbede, Oluwasanmi Moses; Sowande, Olusiji Sunday; Anele, Uchenna Young; Oni, Oluwakemi Oluremilekun; Onwuka, Chryss Friday Ijeoma; Onifade, Olufemi Sunday; Yusuf, Kafayat Omowumi; Dele, Peter Aniwe; Aderinboye, Ronke Yemisi

2011-07-09

448

Atmospheric chemistry research  

SciTech Connect

Global environmental changes are occurring all around us, and the energy industry is a major player in the changes that are taking place. Wise energy policy can only be generated from a position of informed enlightenment and understanding about the environmental consequences of energy production and utilization. The atmospheric chemistry research being conducted at the University of Kentucky's Center for Applied Energy Research is geared toward providing the knowledge necessary to allow industrial and legislative officials to make responsible energy decisions in the 1990's and beyond. Three programs are described: the Kentucky Acid Deposition Program Precipitation chemistry network; modeling of regional and urban photochemistry and acid deposition; and modeling of global tropospheric chemistry.

Saylor, R.D. (Center for Applied Energy Research, Lexington, KY (USA))

1990-01-01

449

Air Composition and Chemistry  

NASA Astrophysics Data System (ADS)

This book is about the atmosphere and humanity's influence on it. For this new edition, Brimblecombe has rewritten and updated much of the book. In the early chapters, he discusses the geochemical, biological and maritime sources of the trace gases. Next, he examines the chemistry of atmospheric gases, suspended particles, and rainfall. After dealing with the natural atmosphere, he examines the sources of air pollution and its effects, with all scenarios updated from the last edition. Scenarios include decline in health, damage to plants and animals, indoor pollution, and acid rain. The final chapters, also revised, are concerned with the chemistry and evolution of the atmospheres of the planets of the solar system. Students with an interest in chemistry and the environmental sciences will find this book highly valuable.

Brimblecombe, Peter

1996-01-01

450

Chemistry in water reactors. Reserapport. (Chemistry in water reactors).  

National Technical Information Service (NTIS)

The international conference Chemistry in Water Reactors was arranged in Nice 24-27/04/1994 by the French Nuclear Energy Society. Examples of technical program areas were primary chemistry, operational experience, fundamental studies and new technology. F...

H. P. Hermansson K. Norring

1994-01-01

451

Collaborative Physical Chemistry Projects Involving Computational Chemistry  

NASA Astrophysics Data System (ADS)

The physical chemistry classes from three colleges have collaborated on two computational chemistry projects using Quantum CAChe 3.0 and Gaussian 94W running on Pentium II PCs. Online communication by email and the World Wide Web was an important part of the collaboration. In the first project, students used molecular modeling to predict benzene derivatives that might be possible hair dyes. They used PM3 and ZINDO calculations to predict the electronic spectra of the molecules and tested the predicted spectra by comparing some with experimental measurements. They also did literature searches for real hair dyes and possible health effects. In the final phase of the project they proposed a synthetic pathway for one compound. In the second project the students were asked to predict which isomer of a small carbon cluster (C3, C4, or C5) was responsible for a series of IR lines observed in the spectrum of a carbon star. After preliminary PM3 calculations, they used ab initio calculations at the HF/6-31G(d) and MP2/6-31G(d) level to model the molecules and predict their vibrational frequencies and rotational constants. A comparison of the predictions with the experimental spectra suggested that the linear isomer of the C5 molecule was responsible for the lines.

Whisnant, David M.; Howe, Jerry J.; Lever, Lisa S.

2000-02-01

452

Revitalizing chemistry laboratory instruction  

NASA Astrophysics Data System (ADS)

This dissertation involves research in three major domains of chemical education as partial fulfillment of the requirements for the Ph.D. program in chemistry at Miami University with a major emphasis on chemical education, and concurrent study in organic chemistry. Unit I, Development and Assessment of a Column Chromatography Laboratory Activity, addresses the domain of Instructional Materials Development and Testing. This unit outlines the process of developing a publishable laboratory activity, testing and revising that activity, and subsequently sharing that activity with the chemical education community. A laboratory activity focusing on the separation of methylene blue and sodium fluorescein was developed to demonstrate the effects of both the stationary and mobile phase in conducting a separation. Unit II, Bringing Industry to the Laboratory, addresses the domain of Curriculum Development and Testing. This unit outlines the development of the Chemistry of Copper Mining module, which is intended for use in high school or undergraduate college chemistry. The module uses the learning cycle approach to present the chemistry of the industrial processes of mining copper to the students. The module includes thirteen investigations (three of which are web-based and ten which are laboratory experiments) and an accompanying interactive CD-ROM, which provides an explanation of the chemistry used in copper mining with a virtual tour of an operational copper mine. Unit III, An Alternative Method of Teaching Chemistry. Integrating Lecture and the Laboratory, is a project that addresses the domain of Research in Student Learning. Fundamental Chemistry was taught at Eastern Arizona College as an integrated lecture/laboratory course that met in two-hour blocks on Monday, Wednesday, and Friday. The students taking this integrated course were compared with students taking the traditional 1-hour lectures held on Monday, Wednesday, and Friday, with accompanying 3-hour lab on Tuesday or Thursday. There were 119 students in the test group, 522 students in the Shelton control group and 556 students in the McBride control group. Both qualitative data and quantitative data were collected. A t-test was used to test significance.

McBride, Phil Blake

453

Chemistry in the Kitchen  

NSDL National Science Digital Library

In this kitchen chemistry activity, learners explore the chemistry of crystals by making sugar crystals, consider a common chemical reaction type responsible for the rising of muffins and cake in the oven, and study the cleaning power of soap. For some of the experiments, a molecular-level view is fostered using structural diagrams of the chemicals, along with a fun "cartoon" version of the reaction with molecules represented by colored shapes (both drawn and cut out). The activity is written to be part of a kit that can be checked out of the library, but can be done without the kit.

Shaw, Maisie; Gomez, Maria

2010-01-01

454

Chemistry WebBook  

National Institute of Standards and Technology Data Gateway

SRD 69 NIST Chemistry WebBook (Web, free access)   The NIST Chemistry WebBook contains: Thermochemical data for over 7000 organic and small inorganic compounds; thermochemistry data for over 8000 reactions; IR spectra for over 16,000 compounds; mass spectra for over 33,000 compounds; UV/Vis spectra for over 1600 compounds; electronic and vibrational spectra for over 5000 compounds; constants of diatomic molecules(spectroscopic data) for over 600 compounds; ion energetics data for over 16,000 compounds; thermophysical property data for 74 fluids.

455

Chemistry in second life.  

PubMed

This review will focus on the current level on chemistry research, education, and visualization possible within the multi-user virtual environment of Second Life. We discuss how Second Life has been used as a platform for the interactive and collaborative visualization of data from molecules and proteins to spectra and experimental data. We then review how these visualizations can be scripted for immersive educational activities and real-life collaborative research. We also discuss the benefits of the social networking affordances of Second Life for both chemists and chemistry students. PMID:19852781

Lang, Andrew S I D; Bradley, Jean-Claude

2009-10-23

456

Chemistry in Second Life  

PubMed Central

This review will focus on the current level on chemistry research, education, and visualization possible within the multi-user virtual environment of Second Life. We discuss how Second Life has been used as a platform for the interactive and collaborative visualization of data from molecules and proteins to spectra and experimental data. We then review how these visualizations can be scripted for immersive educational activities and real-life collaborative research. We also discuss the benefits of the social networking affordances of Second Life for both chemists and chemistry students.

Lang, Andrew SID; Bradley, Jean-Claude

2009-01-01

457

Chemistry in cometary comae.  

PubMed

Significant gas-phase chemistry occurs in the comae of bright comets, as is demonstrated here for the case of Comet Hale-Bopp. The abundance ratio of the two isomers, hydrogen cyanide and hydrogen isocyanide, is shown to vary with heliocentric distance in a way that is consistent with production of HNC by ion-molecule chemistry initiated by the photoionization of water. Likewise, the first maps of emission from HCO+ show an abundance and an extended distribution that are consistent with the same chemical model. PMID:9809016

Irvine, W M; Dickens, J E; Lovell, A J; Schloerb, F P; Senay, M; Bergin, E A; Jewitt, D; Matthews, H E

1998-01-01

458

Chemistry of Transactinides  

NASA Astrophysics Data System (ADS)

In this chapter, the chemical properties of the man-made transactinide elements rutherfordium, Rf (element 104), dubnium, Db (element 105), seaborgium, Sg (element 106), bohrium, Bh (element 107), hassium, Hs (element 108), and copernicium, Cn (element 112) are reviewed, and prospects for chemical characterizations of even heavier elements are discussed. The experimental methods to perform rapid chemical separations on the time scale of seconds are presented and comments are given on the special situation with the transactinides where chemistry has to be studied with single atoms. It follows a description of theoretical predictions and selected experimental results on the chemistry of elements 104 through 108, and element 112.

Kratz, J. V.

459

The concentrations of methaemoglogin, carboxyhaemoglobin and some haematological parameters in tobacco snuff addicts in Igbo of Nigeria.  

PubMed

Methaemoglobin, carboxyhaemoglobin concentrations and some haematological parameters were studied in fifty tobacco snuff addicts (40 males and 10 females) in some villages of Anambra State, Nigeria. The aim was to investigate possible adverse effects of tobacco snuff in addicts in Igbos of Anambra State. Fifty apparently healthy persons (25 males and 25 females) who do not inhale snuff were used as controls. The age range of tests and control subjects was 25-65 years. The results showed no statistically significant difference when the tests group was compared with the control group. A comparison of the results on the basis of sex, age and period of exposure, showed no significant differences. Blood picture in test and control groups was normocytic and normochromic. The results suggest that tobacco snuff may not have any adverse effect on haemoglobin metabolism and erythropoiesis. PMID:18379614

Ureme, S O; Ibeagha, I D; Maduka, I G; Ibeagbulam, O G

460

Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: definitions and current practice in Europe.  

PubMed

The Accreditation Subcommittee of the EBMT regularly publishes special reports on current practice of haemopoietic stem cell transplantation for haematological diseases, solid tumours and immune disorders in Europe. Major changes have occurred since the first report was published in 1996. Haemopoietic stem cell transplantation today includes grafting with allogeneic and autologous stem cells derived from bone marrow, peripheral blood and cord blood. With reduced intensity conditioning regimens in allogeneic transplantation, the age limit has increased, permitting the inclusion of older patients. New indications have emerged such as autoimmune disorders and AL amyloidosis for autologous, and solid tumours for allogeneic transplants. The introduction of alternative therapies has challenged well-established indications such as imatinib for chronic myeloid leukaemia. An updated report with revised tables and operating definitions is presented here. PMID:16444286

Ljungman, P; Urbano-Ispizua, A; Cavazzana-Calvo, M; Demirer, T; Dini, G; Einsele, H; Gratwohl, A; Madrigal, A; Niederwieser, D; Passweg, J; Rocha, V; Saccardi, R; Schouten, H; Schmitz, N; Socie, G; Sureda, A; Apperley, J

2006-03-01

461

Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: current practice in Europe 2009.  

PubMed

The European Group for Blood and Marrow Transplantation regularly publishes special reports on the current practice of haematopoietic SCT for haematological diseases, solid tumours and immune disorders in Europe. Major changes have occurred since the first report was published. HSCT today includes grafting with allogeneic and autologous stem cells derived from BM, peripheral blood and cord blood. With reduced-intensity conditioning regimens in allogeneic transplantation, the age limit has increased, permitting the inclusion of older patients. New indications have emerged, such as autoimmune disorders and AL amyloidosis for autologous HSCT and solid tumours, myeloproliferative syndromes and specific subgroups of lymphomas for allogeneic transplants. The introduction of alternative therapies, such as imatinib for CML, has challenged well-established indications. An updated report with revised tables and operating definitions is presented. PMID:19584824

Ljungman, P; Bregni, M; Brune, M; Cornelissen, J; de Witte, T; Dini, G; Einsele, H; Gaspar, H B; Gratwohl, A; Passweg, J; Peters, C; Rocha, V; Saccardi, R; Schouten, H; Sureda, A; Tichelli, A; Velardi, A; Niederwieser, D

2009-07-06

462

Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: definitions and current practice in Europe.  

PubMed

The Accreditation Sub-Committee of the EBMT regularly publishes special reports on current practice of haemopoietic stem cell transplantation for haematological diseases, solid tumours and immune disorders. Major changes have occurred since the last report in 1998. Haemopoietic stem cell transplantation today includes allogeneic and autologous stem cells derived from bone marrow, peripheral blood and cord blood. With reduced intensity conditioning regimens in allogeneic transplantation, the age limit has increased, permitting the inclusion of older patients. New indications have emerged, such as autoimmune disorders and AL amyloidosis for autologous, and solid tumours for allogeneic transplants. Other indications, such as autologous transplantation for breast cancer have been challenged. An updated report with revised tables and operating definitions is presented here. PMID:12180107

Urbano-Ispizua, A; Schmitz, N; de Witte, T; Frassoni, F; Rosti, G; Schrezenmeier, H; Gluckman, E; Friedrich, W; Cordonnier, C; Socie, G; Tyndall, A; Niethammer, D; Ljungman, P; Gratwohl, A; Apperley, J; Niederwieser, D; Bacigalupo, A

2002-04-01

463

Chemistry: Experiments, Demonstrations and Other Activities Suggested for Chemistry.  

ERIC Educational Resources Information Center

This publication is a handbook used in conjunction with the course of study in chemistry developed through the New York State Education Department and The University of the State of New York. It contains experiments, demonstrations, and other activities for a chemistry course. Areas covered include the science of chemistry, the atomic structure of…

New York State Education Dept., Albany. Bureau of Secondary Curriculum Development.

464

Connected Chemistry--Incorporating Interactive Simulations into the Chemistry Classroom.  

ERIC Educational Resources Information Center

|Describes a novel modeling and simulation package and assesses its impact on students' understanding of chemistry. Connected Chemistry was implemented inside the NetLogo modeling environment. Using Connected Chemistry, students employed problem -solving techniques characterized by stronger attempts at conceptual understanding and logical…

Stieff, Mike; Wilensky, Uri

2003-01-01

465

Chlorpyrifos-induced changes in oxidant/antioxidant status and haematological parameters of Cyprinus carpio carpio: ameliorative effect of lycopene.  

PubMed

The present study was carried out to investigate the potential ameliorative effects of lycopene against chlorpyrifos (CPF) toxicity in carp. The fish were divided into 7 different experimental groups and received the following treatments: Group 1, control; Group 2, orally administered corn oil; Group 3, oral lycopene (10 mg kg(-1) body weight); Group 4, exposure to 0.040 mg L(-1) CPF; Group 5, exposure to 0.040 mg L(-1) CPF plus oral administration of 10 mg kg(-1) lycopene; Group 6, exposure to 0.080 mg L(-1) CPF; and Group 7, exposure to 0.040 mg L(-1) CPF plus oral administration of 10 mg kg(-1) lycopene. Treatment was continued for 14 d and samples of the blood and tissue (liver, kidney, and gill) were collected at the end of the experiment and analysed for their oxidant-antioxidant status, including the malondialdehyde (MDA) levels, and the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity. The samples were also measured for changes in the haematological parameters, such as the red blood cell (RBC) and white blood cell (WBC) counts, the haemoglobin concentration (Hb), the haematocrit (Ht) level, and the erythrocyte indices: the mean corpuscular volume (MCV), the mean corpuscular haemoglobin (MCH) and the mean corpuscular haemoglobin concentration (MCHC). The findings of this study demonstrated that CPF had a negative effect on the haematological parameters and the antioxidant enzyme activities of the fish; this toxic effect was neutralised by the administration of lycopene. The present results suggest that lycopene (10 mg kg(-1)) can be effective in the protection of CPF-induced toxicity in fish. PMID:23312461

Ural, Mevlüt ?ener

2013-01-09

466

The protective effect of hydrated sodium calcium aluminosilicate against haematological, biochemical and pathological changes induced by Zearalenone in mice.  

PubMed

Hydrated sodium calcium aluminosilicate (HSCAS), an anticaking agent for mixed feed, was added alone or simultaneously with a toxic Zearalenone (ZEN) dose to balb/c mice and was evaluated for its ability to restore damages induced by ZEN. The latter is a mycotoxin produced by fusarium genera; it is mainly known to induce several toxic effects such as hepatotoxicity, immunotoxicity and nephrotoxicity on animals and humans. The experimental approach consisted of eight treatments of six mice each by 400 mg/kg bw or 5 g/kg bw of HSCAS. Two experimental groups have received respectively ZEN alone at 40 (8% of LD50) and at 500 mg/kg bw (LD50). Two other groups have received ZEN at 40 or 500 mg/kg bw combined respectively with HSCAS at 400 mg/kg bw and 5 g/kg bw. The control groups received water or olive oil. Forty-eight hours after treatment, blood samples were collected for haematological and serum biochemical parameters measurements. ZEN treatment significantly increased hematocrit, haemoglobin, white blood cells: lymphocytes, eosinophils, neutrophils, monocytes and the most of biochemical serum parameters; it significantly reduced platelets and induced degenerative changes in the hepatic and renal tissues; while, the mixture of HSCAS with ZEN induced a reestablishment of haematological parameters, levels of serum biochemical enzyme activities and histological pictures of both liver and kidney. It also prevented general toxicity of ZEN. This was observed by the shift of LD50 for this toxin. Thus, our data strongly suggested that deleterious effects of ZEN could be overcome or, at least, significantly were diminished by HSCAS. Moreover, this sorbent by itself did not show any toxic effects. PMID:16563452

Abbès, Samir; Ouanes, Zouhour; ben Salah-Abbès, Jalila; Houas, Zohra; Oueslati, Ridha; Bacha, Hassen; Othman, Omar

2006-03-24

467

Organic Chemistry, 5th Edition  

NSDL National Science Digital Library

Finds ChemEd DL resources related to the sections of the Organic Chemistry textbook, Organic Chemistry, 5th Edition, by William H. Brown, Christopher S. Foote, Brent L. Iverson, Eric Anslyn published by Brooks/Cole, 2009.

468

The Lighter Side of Chemistry.  

ERIC Educational Resources Information Center

Discusses the rationale for using photochemistry to merge descriptive chemistry and molecular orbital theory in first-year chemistry courses. Includes procedures and safety information for various activities, demonstrations, and experiments involving photochemical reactions. (DH)

Lamb, William G.

1984-01-01

469

The Art of Teaching Chemistry.  

ERIC Educational Resources Information Center

|Contains descriptions of 11 college chemistry education programs that were awarded grants by the Pfizer Foundation because they make the introductory chemistry experience more positive and engaging for students, especially women and minorities. (LZ)|

Lloyd, Baird W., Ed.

1994-01-01

470

Special Report: Chemistry of Comets.  

ERIC Educational Resources Information Center

|Discusses the chemistry of comets. How comets provide clues to the birth of the solar system, photolytic reactions on comets involving water, chemical modeling, nuclear chemistry, and research findings are among the areas considered. (JN)|

A'Hearn, Michael F.

1984-01-01

471

In vitro activities of amphotericin B and AmBisome against Aspergillus isolates recovered from Italian patients treated for haematological malignancies.  

PubMed

Although there is evidence that liposomal amphotericin B (AmBisome) is non-inferior to amphotericin B (AmB) in terms of in vivo efficacy, in vitro data regarding the activity of AmBisome against clinical isolates of Aspergillus are rare. In this study, the susceptibilities to AmB and AmBisome of 103 Aspergillus complex isolates (48 Aspergillus flavus, 33 Aspergillus fumigatus, 13 Aspergillus terreus and 9 Aspergillus niger) recovered from haematological patients with invasive infection were compared. Minimum inhibitory concentrations (MICs) were determined by the broth microdilution (BMD) method according to the Clinical and Laboratory Standards Institute (CLSI), whilst AmB susceptibility was also determined by Etest. Using a susceptible/resistant MIC cut-off of 1mg/L, all A. fumigatus and A. niger complexes isolates were susceptible to both AmB and AmBisome. In contrast, 38.5% and 30.8% of the A. terreus complex isolates were resistant to AmB and AmBisome, respectively, with good agreement between BMD and Etest methods. With respect to A. flavus complex isolates, 43.7% and 16.7% were resistant by the BMD method to AmBisome and AmB, respectively. For isolates with discrepant results, AmB MICs obtained by Etest were higher than those obtained for AmB by the BMD method and they were closer to those obtained for AmBisome by BMD. Aspergillus flavus AmB MICs ranged from 0.5 mg/L to 2 mg/L by the BMD method and from 1 mg/L to >16 mg/L by the Etest method, and AmBisome MICs ranged from 0.06 mg/L to >16 mg/L by the BMD method. Etest appears to be superior to the CLSI BMD method using AmB in detecting AmB resistance of Aspergillus spp., although the CLSI BMD method might be a suitable procedure if AmBisome is used as the test drug. PMID:22429723

Colozza, Camilla; Posteraro, Brunella; Santilli, Stefania; De Carolis, Elena; Sanguinetti, Maurizio; Girmenia, Corrado

2012-03-17

472

Chemistry of soil aluminum  

Microsoft Academic Search

Better understanding of soil aluminum has had dramatic effects on the interpretation of many aspects of soil chemistry. Aluminum is a Group III element, metallic in nature, and exhibits both ionic and cuvaient bonding. It is the most plentiful of all metallic cations of the earth's crust. It is released from octahedral coordination with oxygen in minerals by weathering processes.

E. O. McLean

1976-01-01

473

Chemistry by the Case  

NSDL National Science Digital Library

This article describes the structure and content of a one-semester course dealing with chemistry, science, and technology designed for nonscience majors. The course uses a hybrid of two active learning methodologies--problem-based team learning and the cas

Dinan, Frank J.

2002-09-01

474

Bioconjugation in pharmaceutical chemistry  

Microsoft Academic Search

Polymer conjugation is of increasing interest in pharmaceutical chemistry for delivering drugs of simple structure or complex compounds such peptides, enzymes and oligonucleotides. For long time drugs, mainly with antitumoral activity, have been coupled to natural or synthetic polymers with the purpose of increasing their blood permanence time, taking advantage of the increased mass that reduces kidney ultrafiltration. However only

F. M. Veronese; M. Morpurgo

1999-01-01