Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis

PubMed Central

Li, Songhua; Samardzija, Marijana; Yang, Zhihui; Grimm, Christian

2016-01-01

RPE65, an abundant membrane-associate protein in the retinal pigment epithelium (RPE), is a key retinoid isomerase of the visual cycle necessary for generating 11-cis-retinal that functions not only as a molecular switch for activating cone and rod visual pigments in response to light stimulation, but also as a chaperone for normal trafficking of cone opsins to the outer segments. Many mutations in RPE65 are associated with Leber congenital amaurosis (LCA). A R91W substitution, the most frequent LCA-associated mutation, results in a severe decrease in protein level and enzymatic activity of RPE65, causing cone opsin mislocalization and early cone degeneration in the mutation knock-in mouse model of LCA. Here we show that R91W RPE65 undergoes ubiquitination-dependent proteasomal degradation in the knock-in mouse RPE due to misfolding. The 26S proteasome non-ATPase regulatory subunit 13 mediated degradation specifically of misfolded R91W RPE65. The mutation disrupted membrane-association and colocalization of RPE65 with lecithin:retinol acyltransferase (LRAT) that provides the hydrophobic substrate for RPE65. Systemic administration of sodium 4-phenylbutyrate (PBA), a chemical chaperone, increased protein stability, enzymatic activity, membrane-association, and colocalization of R91W RPE65 with LRAT. This rescue effect increased synthesis of 11-cis-retinal and 9-cis-retinal, a functional iso-chromophore of the visual pigments, led to alleviation of S-opsin mislocalization and cone degeneration in the knock-in mice. Importantly, PBA-treatment also improved cone-mediated vision in the mutant mice. These results indicate that PBA, a U.S. Food and Drug Administration-approved safe oral medication, may provide a noninvasive therapeutic intervention that delays daylight vision loss in patients with RPE65 mutations. SIGNIFICANCE STATEMENT LCA is a severe early onset retinal dystrophy. Recent clinical trials of gene therapy have implicated the need of an alternative or

Discovery of natural mouse serum derived HIV-1 entry inhibitor(s).

PubMed

Wei, M; Chen, Y; Xi, J; Ru, S; Ji, M; Zhang, D; Fang, Q; Tang, B

Among rationally designed human immunodeficiency virus 1 (HIV-1) inhibitors, diverse natural factors have showed as potent anti-HIV activity in human blood. We have discovered that the boiled supernatant of healthy mouse serum could suppress HIV-1 entry, and exhibited reduced inhibitory activity after trypsin digestion. Further analysis demonstrated that only the fraction containing 10-25 K proteins could inhibit HIV-1 mediated cell-cell fusion. These results suggest that the 10-25 K protein(s) is novel natural HIV-1 entry inhibitor(s). Our findings provide important information about novel natural HIV entry inhibitors in mouse serum.

Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria.

PubMed

Salzman, Nita H; de Jong, Hendrik; Paterson, Yvonne; Harmsen, Hermie J M; Welling, Gjalt W; Bos, Nicolaas A

2002-11-01

Total genomic DNA from samples of intact mouse small intestine, large intestine, caecum and faeces was used as template for PCR amplification of 16S rRNA gene sequences with conserved bacterial primers. Phylogenetic analysis of the amplification products revealed 40 unique 16S rDNA sequences. Of these sequences, 25% (10/40) corresponded to described intestinal organisms of the mouse, including Lactobacillus spp., Helicobacter spp., segmented filamentous bacteria and members of the altered Schaedler flora (ASF360, ASF361, ASF502 and ASF519); 75% (30/40) represented novel sequences. A large number (11/40) of the novel sequences revealed a new operational taxonomic unit (OTU) belonging to the Cytophaga-Flavobacter-Bacteroides phylum, which the authors named 'mouse intestinal bacteria'. 16S rRNA probes were developed for this new OTU. Upon analysis of the novel sequences, eight were found to cluster within the Eubacterium rectale-Clostridium coccoides group and three clustered within the Bacteroides group. One of the novel sequences was distantly related to Verrucomicrobium spinosum and one was distantly related to Bacillus mycoides. Oligonucleotide probes specific for the 16S rRNA of these novel clones were generated. Using a combination of four previously described and four newly designed probes, approximately 80% of bacteria recovered from the murine large intestine and 71% of bacteria recovered from the murine caecum could be identified by fluorescence in situ hybridization (FISH).

[PKA-regulated phosphorylation status of S149 and S321 sites of CDC25B inhibits mitosis of fertilized mouse eggs].

PubMed

Xiao, Jian-Ying; Liu, Chao; Sun, Xiao-Han; Yu, Bing-Zhi

2012-02-25

To further test whether protein kinase A (PKA) can affect the mitotic cell cycle, one-cell stage mouse embryos at S phase (22 h after hCG injection) were incubated in M16 medium containing various concentrations of H-89, a PKA inhibitor. With increasing concentrations of H-89 (0-50 μmol/L), the G(2) phase of eggs was decreased and the cleavage rate was accelerated. A concentration of 40 μmol/L H-89 led to all of the mouse eggs entering the M phase of mitosis. Furthermore, to study the role of PKA in regulating the phosphorylation status of S149 and S321 sites of cell division cycle 25B (CDC25B) on one-cell stage fertilized mouse eggs, pBSK-CDC25B-WT, pBSK-CDC25B-S149A, pBSK-CDC25B-S321A and pBSK-CDC25B-S149A/S321A were transcribed into mRNAs in vitro, then mRNAs were microinjected into S phase of mouse fertilized eggs and cultured in M16 medium pretreated with H-89. Then, the cleavage of fertilized eggs, maturation promoting factor (MPF) activity and phosphorylation status of CDC2-Tyr15 were observed. In the presence of 40 μmol/L H-89, the cleavage rate of fertilized eggs in CDC25B-S/A-mRNAs and CDC25B-WT-mRNA injected groups was significantly higher than that in the control groups, and the peak of MPF activity appeared in the CDC25B-S/A-mRNAs and CDC25B-WT-mRNA injected groups earlier than that in the control groups. CDC2-Tyr15 phosphorylation state was consistent with MPF activity. In conclusion, the present study suggests that PKA regulates the early development of mouse embryos by phosphorylation of S149 and S321 of CDC25B, which plays an important role in the regulation of G(2)/M transition in the mitotic cell cycle of fertilized mouse eggs.

Role of blood ribosomal protein S19 in coagulum resorption: a study using Gln137Glu-ribosomal protein S19 gene knock-in mouse.

PubMed

Chen, Jun; Fujino, Rika; Zhao, Rui; Semba, Umeko; Araki, Kimi; Yamamoto, Tetsuro

2014-11-01

Sera of human, guinea pig or mouse contain a strong monocyte chemoattractant capacity that is attributed to the ribosomal protein S19 (RP S19) oligomers generated during blood coagulation. In contrast, sera prepared from Gln137Glu-RP S19 gene knock-in mice contained negligible chemoattractant capacity. When coagula that had been pre-formed from the blood of both the wild type and knock-in mice were intraperitoneally inserted into host mice, after 3 days of recovery, the knock-in mouse coagula remained larger than the wild type mouse coagula. The wild type mouse coagula were covered by multiple macrophage layers at the surface and were infiltrated inside by macrophages. Knock-in mouse coagula exhibited less macrophage involvement. When coagula of knock-in mice and coagula of knock-in mice containing C5a/RP S19, an artificial substitute of the RP S19 oligomers, were intraperitoneally inserted as pairs, the C5a/RP S19 containing coagulum was more rapidly absorbed, concomitant with increased macrophage involvement. Finally, when the knock-in mouse and wild type mouse coagula pairs were inserted into mice in which macrophages had been depleted using clodronate liposome, the size difference of recovered coagula was reversed. These results indicate the importance of the RP S19 oligomer-induced macrophage recruitment in coagulum resorption. © 2014 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.

14 CFR 91.861 - Base level.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Base level. 91.861 Section 91.861... level. (a) U.S. Operators. The base level of a U.S. operator is equal to the number of owned or leased... to paragraphs (a) (1) and (2). (1) The base level of a U.S. operator shall be increased by a number...

14 CFR 91.211 - Supplemental oxygen.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Supplemental oxygen. 91.211 Section 91.211... Requirements § 91.211 Supplemental oxygen. (a) General. No person may operate a civil aircraft of U.S. registry... the required minimum flight crew is provided with and uses supplemental oxygen for that part of the...

14 CFR 91.211 - Supplemental oxygen.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Supplemental oxygen. 91.211 Section 91.211... Requirements § 91.211 Supplemental oxygen. (a) General. No person may operate a civil aircraft of U.S. registry... the required minimum flight crew is provided with and uses supplemental oxygen for that part of the...

14 CFR 91.211 - Supplemental oxygen.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Supplemental oxygen. 91.211 Section 91.211... Requirements § 91.211 Supplemental oxygen. (a) General. No person may operate a civil aircraft of U.S. registry... the required minimum flight crew is provided with and uses supplemental oxygen for that part of the...

14 CFR 91.211 - Supplemental oxygen.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Supplemental oxygen. 91.211 Section 91.211... Requirements § 91.211 Supplemental oxygen. (a) General. No person may operate a civil aircraft of U.S. registry... the required minimum flight crew is provided with and uses supplemental oxygen for that part of the...

14 CFR 91.211 - Supplemental oxygen.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Supplemental oxygen. 91.211 Section 91.211... Requirements § 91.211 Supplemental oxygen. (a) General. No person may operate a civil aircraft of U.S. registry... the required minimum flight crew is provided with and uses supplemental oxygen for that part of the...

40 CFR 91.408 - Pre-test procedures.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 21 2012-07-01 2012-07-01 false Pre-test procedures. 91.408 Section 91...) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.408 Pre-test... accordance with § 91.117. (b) Engine pre-test preparation. (1) Drain and charge the fuel tank(s) with the...

40 CFR 91.408 - Pre-test procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Pre-test procedures. 91.408 Section 91...) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.408 Pre-test... accordance with § 91.117. (b) Engine pre-test preparation. (1) Drain and charge the fuel tank(s) with the...

40 CFR 91.408 - Pre-test procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Pre-test procedures. 91.408 Section 91...) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.408 Pre-test... accordance with § 91.117. (b) Engine pre-test preparation. (1) Drain and charge the fuel tank(s) with the...

40 CFR 91.408 - Pre-test procedures.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Pre-test procedures. 91.408 Section 91...) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.408 Pre-test... accordance with § 91.117. (b) Engine pre-test preparation. (1) Drain and charge the fuel tank(s) with the...

40 CFR 91.408 - Pre-test procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Pre-test procedures. 91.408 Section 91...) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.408 Pre-test... accordance with § 91.117. (b) Engine pre-test preparation. (1) Drain and charge the fuel tank(s) with the...

45 CFR 96.91 - Audit requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Audit requirement. 96.91 Section 96.91 Public... Block Grants § 96.91 Audit requirement. Pursuant to section 1745(b) of the Reconciliation Act (31 U.S.C. 1243 note) an audit is required with respect to the 2-year period beginning on October 1, 1981, and...

40 CFR 91.412 - Data logging.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Data logging. 91.412 Section 91.412... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.412 Data logging. (a) A computer or any other automatic data collection (ADC) device(s) may be used as long as the system meets the...

40 CFR 91.412 - Data logging.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Data logging. 91.412 Section 91.412... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.412 Data logging. (a) A computer or any other automatic data collection (ADC) device(s) may be used as long as the system meets the...

40 CFR 91.412 - Data logging.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 21 2012-07-01 2012-07-01 false Data logging. 91.412 Section 91.412... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.412 Data logging. (a) A computer or any other automatic data collection (ADC) device(s) may be used as long as the system meets the...

40 CFR 91.412 - Data logging.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Data logging. 91.412 Section 91.412... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.412 Data logging. (a) A computer or any other automatic data collection (ADC) device(s) may be used as long as the system meets the...

40 CFR 91.412 - Data logging.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Data logging. 91.412 Section 91.412... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.412 Data logging. (a) A computer or any other automatic data collection (ADC) device(s) may be used as long as the system meets the...

49 CFR 91.3 - Investigations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Investigations. 91.3 Section 91.3 Transportation Office of the Secretary of Transportation INTERNATIONAL AIR TRANSPORTATION FAIR COMPETITIVE PRACTICES...), on complaint of any U.S. flag air carrier or on their own initiative, shall investigate: (a...

Purification and Characterization of Mouse Soluble Receptor for Advanced Glycation End Products (sRAGE)*

PubMed Central

Hanford, Lana E.; Enghild, Jan J.; Valnickova, Zuzana; Petersen, Steen V.; Schaefer, Lisa M.; Schaefer, Todd M.; Reinhart, Todd A.; Oury, Tim D.

2007-01-01

The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily of cell surface proteins that has been implicated as a progression factor in a number of pathologic conditions from chronic inflammation to cancer to Alzheimer’s disease. In such conditions, RAGE acts to facilitate pathogenic processes. Its secreted isoform, soluble RAGE or sRAGE, has the ability to prevent RAGE signaling by acting as a decoy. sRAGE has been used successfully in animal models of a range of diseases to antagonize RAGE-mediated pathologic processes. In humans, sRAGE results from alternative splicing of RAGE mRNA. This study was aimed to determine whether the same holds true for mouse sRAGE and, in addition, to biochemically characterize mouse sRAGE. The biochemical characteristics examined include glycosylation and disulfide patterns. In addition, sRAGE was found to bind heparin, which may mediate its distribution in the extracellular matrix and cell surfaces of tissues. Finally, our data indicated that sRAGE in the mouse is likely produced by carboxyl-terminal truncation, in contrast to the alternative splicing mechanism reported in humans. PMID:15381690

Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract.

PubMed

Hanaue, Mayu; Miwa, Naofumi; Uebi, Tatsuya; Fukuda, Yusuke; Katagiri, Yukiko; Takamatsu, Ken

2011-02-01

We recently found that Xenopus dicalcin, present in the extracellular egg-coating envelope, suppresses the efficiency of fertilization in vitro through binding to envelope-constituent glycoproteins. In the present study, we explored the mouse counterpart of Xenopus dicalcin, specifically its localization in the female reproductive tract and its action on mouse fertilization. Our homology and phylogenetic analyses using known S100 proteins showed that S100A11 is most closely related to Xenopus dicalcin. S100A11 was localized in the cytosol of luteal cells, but not in the follicle, in the mouse ovary, and also in the cytosol of the oviductal epithelial cells. In addition, our quantitative analyses revealed preferential expression of S100A11 in the ampullary region of the oviduct and at the estrus stage during the mouse estrous cycle. In the cumulus cell-oocyte complex dissected from the oviduct following ovulation, S100A11 was present in the plasma membrane of cumulus cells, but not in the zona pellucida, which is comparable with Ca(2+) -dependent binding of exogenously applied S100A11 to the plasma membrane of cumulus cells. Pretreatment of the cumulus cell-oocyte complex with recombinant S100A11 substantially reduced the efficiency of in vitro fertilization, but S100A10, the next closest S100 protein to Xenopus dicalcin, had no effect. These results suggested that S100A11 is the mouse counterpart of Xenopus dicalcin, suppresses the fertilization process through its action on cumulus cells, and thereby plays a key role in fertilization success in the mouse. Copyright © 2010 Wiley-Liss, Inc.

19 CFR 181.91 - Applicability.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 19 Customs Duties 2 2013-04-01 2013-04-01 false Applicability. 181.91 Section 181.91 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY... provisions of this subpart whenever the requested ruling involves a subject matter specified in § 181.92(b)(6...

19 CFR 181.91 - Applicability.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 19 Customs Duties 2 2012-04-01 2012-04-01 false Applicability. 181.91 Section 181.91 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY... provisions of this subpart whenever the requested ruling involves a subject matter specified in § 181.92(b)(6...

19 CFR 181.91 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 19 Customs Duties 2 2010-04-01 2010-04-01 false Applicability. 181.91 Section 181.91 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY... provisions of this subpart whenever the requested ruling involves a subject matter specified in § 181.92(b)(6...

19 CFR 181.91 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 19 Customs Duties 2 2014-04-01 2014-04-01 false Applicability. 181.91 Section 181.91 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY... provisions of this subpart whenever the requested ruling involves a subject matter specified in § 181.92(b)(6...

19 CFR 181.91 - Applicability.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 19 Customs Duties 2 2011-04-01 2011-04-01 false Applicability. 181.91 Section 181.91 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY... provisions of this subpart whenever the requested ruling involves a subject matter specified in § 181.92(b)(6...

28 CFR 91.60 - Grantee's responsibilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Grantee's responsibilities. 91.60 Section 91.60 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) GRANTS FOR CORRECTIONAL FACILITIES...'s responsibilities. Specific duties. As part of its role in the NEPA process, the grantee agency...

10 CFR 9.1 - Scope and purpose.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 1 2014-01-01 2014-01-01 false Scope and purpose. 9.1 Section 9.1 Energy NUCLEAR... Freedom of Information Act, 5 U.S.C. 552, concerning the availability to the public of Nuclear Regulatory... 1974, 5 U.S.C. 552a, concerning disclosure and availability of certain Nuclear Regulatory Commission...

10 CFR 9.1 - Scope and purpose.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 1 2012-01-01 2012-01-01 false Scope and purpose. 9.1 Section 9.1 Energy NUCLEAR... Freedom of Information Act, 5 U.S.C. 552, concerning the availability to the public of Nuclear Regulatory... 1974, 5 U.S.C. 552a, concerning disclosure and availability of certain Nuclear Regulatory Commission...

10 CFR 9.1 - Scope and purpose.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 1 2013-01-01 2013-01-01 false Scope and purpose. 9.1 Section 9.1 Energy NUCLEAR... Freedom of Information Act, 5 U.S.C. 552, concerning the availability to the public of Nuclear Regulatory... 1974, 5 U.S.C. 552a, concerning disclosure and availability of certain Nuclear Regulatory Commission...

10 CFR 9.1 - Scope and purpose.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Scope and purpose. 9.1 Section 9.1 Energy NUCLEAR... Freedom of Information Act, 5 U.S.C. 552, concerning the availability to the public of Nuclear Regulatory... 1974, 5 U.S.C. 552a, concerning disclosure and availability of certain Nuclear Regulatory Commission...

10 CFR 9.1 - Scope and purpose.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Scope and purpose. 9.1 Section 9.1 Energy NUCLEAR... Freedom of Information Act, 5 U.S.C. 552, concerning the availability to the public of Nuclear Regulatory... 1974, 5 U.S.C. 552a, concerning disclosure and availability of certain Nuclear Regulatory Commission...

14 CFR 91.615-91.699 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-01-01

...) AIR TRAFFIC AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Additional Equipment and Operating Requirements for Large and Transport Category Aircraft §§ 91.615-91.699 [Reserved] ...

Properties of 91 Southern Soil Series

Treesearch

Basil D. Doss; W. M. Broadfoot

1956-01-01

From June 1954 to July 1955 the Vicksburg Infiltration Project collected and analyzed samples of 91 soil series in 7 southern states. The purpose was to supply the U. S. Army with information needed for specialized research on military trafficability, but the basic data on soil properties should be of interest to soil scientists generally. The 91 series may be...

49 CFR 91.5 - Findings and recommendations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Findings and recommendations. 91.5 Section 91.5 Transportation Office of the Secretary of Transportation INTERNATIONAL AIR TRANSPORTATION FAIR COMPETITIVE... excessive or otherwise discriminatory charges against U.S. flag air carriers or causes such carriers to be...

Cloning, sequencing and expression in MEL cells of a cDNA encoding the mouse ribosomal protein S5.

PubMed

Vanegas, N; Castañeda, V; Santamaría, D; Hernández, P; Schvartzman, J B; Krimer, D B

1997-06-05

We describe the isolation and characterization of a cDNA encoding the mouse S5 ribosomal protein. It was isolated from a MEL (murine erythroleukemia) cell cDNA library by differential hybridization as a down regulated sequence during HMBA-induced differentiation. Northern series analysis showed that S5 mRNA expression is reduced 5-fold throughout the differentiation process. The mouse S5 mRNA is 760 bp long and encodes for a 204 amino acid protein with 94% homology with the human and rat S5.

Behavioral assays with mouse models of Alzheimer’s disease: practical considerations and guidelines

PubMed Central

Puzzo, Daniela; Lee, Linda; Palmeri, Agostino; Calabrese, Giorgio; Arancio, Ottavio

2014-01-01

In Alzheimer’s disease (AD) basic research and drug discovery, mouse models are essential resources for uncovering biological mechanisms, validating molecular targets and screening potential compounds. Both transgenic and non-genetically modified mouse models enable access to different types of AD-like pathology in vivo. Although there is a wealth of genetic and biochemical studies on proposed AD pathogenic pathways, as a disease that centrally features cognitive failure, the ultimate readout for any interventions should be measures of learning and memory. This is particularly important given the lack of knowledge on disease etiology – assessment by cognitive assays offers the advantage of targeting relevant memory systems without requiring assumptions about pathogenesis. A multitude of behavioral assays are available for assessing cognitive functioning in mouse models, including ones specific for hippocampal-dependent learning and memory. Here we review the basics of available transgenic and non-transgenic AD mouse models and detail three well-established behavioral tasks commonly used for testing hippocampal-dependent cognition in mice – contextual fear conditioning, radial arm water maze and Morris water maze. In particular, we discuss the practical considerations, requirements and caveats of these behavioral testing paradigms. PMID:24462904

Optimization of intracerebral tumour protection by active-specific immunization against murine melanoma B16/G3.12.

PubMed

Staib, L; Harel, W; Mitchell, M S

2001-08-01

Development of brain metastases despite extracerebral response to systemic immunotherapy is a common problem in melanoma patients. We have previously described a murine melanoma vaccine of interferon-gamma (IFNgamma)-treated, irradiated syngeneic B16/G3.12 and allogeneic (Cloudman) melanoma cells, plus the adjuvant DETOX, that is protective against subcutaneous (93%) or intracerebral (69%) syngeneic challenge. This study aimed to optimize this vaccine. Groups of nine or 10 mice were immunized five times in 5 weeks with: (i) complete vaccine +/- IFNgamma (VAC+, VAC-); (ii) syngeneic 2 x 106 G3.12 cells plus DETOX (Syn+D), (iii) 2 x 106 allogeneic Cloudman cells plus DETOX (Allo+D); (iv) VAC+ without DETOX (no DETOX); (v) DETOX alone (DETOX); or (vi) phosphate buffered saline (PBS). Mice were challenged subcutaneously with 104 viable G3.12 (or Cloudman cells) and after 35 days intracerebrally with 104 G3.12 cells. Expression of H-2 antigens (measured using fluorescence-activated cell sorting), splenocyte cytotoxicity (measured using 51Cr release) and median overall survival (OAS) were analysed using the log-rank test. VAC+, VAC- and G3.12 mice were equally protected from subcutaneous (s.c.) and intracerebral (i.c.) melanoma challenge (OAS 65 days for s.c., 30 days for i.c.). Protection was less (P < 0.05) in DETOX mice (48 days for s.c.), PBS mice (47 days for s.c., 21 days for i.c.) or no DETOX mice (51 days for s.c.). Allo+D mice showed s.c. (59 days) but not i.c. protection (20 days). IFNgamma incubation did not increase the effect in either the challenge cells or the vaccine cells (P > 0.05). Specific cytotoxicity was seen with G3.12 targets in VAC+ (27%) but not PBS (2%; P < 0.05) mice with equal NK (YAC-1) lysis (10% versus 7%; P< 0.05). Optimal protection against s.c./i.c. experimental murine melanoma was yielded by irradiated syngeneic cells plus DETOX. DETOX alone was not active. Upregulation of H-2 antigens with IFNgamma under these conditions does not

Inhibitor(s) of the classical complement pathway in mouse serum limit the utility of mice as experimental models of neuromyelitis optica.

PubMed

Ratelade, Julien; Verkman, A S

2014-11-01

Neuromyelitis optica (NMO) is an inflammatory demyelinating disease of the central nervous system in which anti-aquaporin-4 (AQP4) autoantibodies (AQP4-IgG) cause damage to astrocytes by complement-dependent cytotoxicity (CDC). Various approaches have been attempted to produce NMO lesions in rodents, some involving genetically modified mice with altered immune cell function. Here, we found that mouse serum strongly inhibits complement from multiple species, preventing AQP4-IgG-dependent CDC. Effects of mouse serum on complement activation were tested in CDC assays in which AQP4-expressing cells were incubated with AQP4-IgG and complement from different species. Biochemical assays and mass spectrometry were used to characterize complement inhibitor(s) in mouse serum. Sera from different strains of mice produced almost no AQP4-IgG-dependent CDC compared with human, rat and guinea pig sera. Remarkably, addition of mouse serum prevented AQP4-IgG-dependent CDC caused by human, rat or guinea pig serum, with 50% inhibition at <5% mouse serum. Hemolysis assays indicated that the inhibitor(s) in mouse serum target the classical and not the alternative complement pathway. We found that the complement inhibitor(s) in mouse serum were contained in a serum fraction purified with protein-A resin; however, the inhibitor was not IgG as determined using serum from IgG-deficient mice. Mass spectrometry on the protein A-purified fraction produced several inhibitor candidates. The low intrinsic complement activity of mouse serum and the presence of complement inhibitor(s) limit the utility of mouse models to study disorders, such as NMO, involving the classical complement pathway. Copyright © 2014 Elsevier Ltd. All rights reserved.

Transplantation of wild-type mouse hematopoietic stem and progenitor cells ameliorates deficits in a mouse model of Friedreich’s ataxia

PubMed Central

Rocca, Celine J.; Goodman, Spencer M.; Dulin, Jennifer N.; Haquang, Joseph H.; Gertsman, Ilya; Blondelle, Jordan; Smith, Janell L. M.; Heyser, Charles J.; Cherqui, Stephanie

2017-01-01

Friedreich’s ataxia (FRDA) is an incurable autosomal recessive neurodegenerative disease caused by reduced expression of the mitochondrial protein frataxin due to an intronic GAA-repeat expansion in the FXN gene. We report the therapeutic efficacy of transplanting wild-type mouse hematopoietic stem and progenitor cells (HSPCs) into the YG8R mouse model of FRDA. In the HSPC-transplanted YG8R mice, development of muscle weakness and locomotor deficits was abrogated as was degeneration of large sensory neurons in the dorsal root ganglia (DRGs) and mitochondrial capacity was improved in brain, skeletal muscle, and heart. Transplanted HSPCs engrafted and then differentiated into microglia in the brain and spinal cord and into macrophages in the DRGs, heart, and muscle of YG8R FRDA mice. We observed the transfer of wild-type frataxin and Cox8 mitochondrial proteins from HSPC-derived microglia/macrophages to FRDA mouse neurons and muscle myocytes in vivo. Our results show the HSPC-mediated phenotypic rescue of FRDA in YG8R mice and suggest that this approach should be investigated further as a strategy for treating FRDA. PMID:29070698

49 CFR 91.9 - Distribution of compensatory funds.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Distribution of compensatory funds. 91.9 Section 91.9 Transportation Office of the Secretary of Transportation INTERNATIONAL AIR TRANSPORTATION FAIR... year, each U.S. flag air carrier which has been subjected to excessive or otherwise discriminatory...

40 CFR 91.603 - Applicability of part 91, subpart F.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Applicability of part 91, subpart F. 91.603 Section 91.603 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Selective Enforcement Auditing...

14 CFR 91.601 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... Requirements for Large and Transport Category Aircraft § 91.601 Applicability. This subpart applies to operation of large and transport category U.S.-registered civil aircraft. ...

Melanoma exosomes deliver a complex biological payload that upregulates PTPN11 to suppress T lymphocyte function.

PubMed

Wu, Yueting; Deng, Wentao; McGinley, Emily Chambers; Klinke, David J

2017-03-01

As exosomes are emerging as a new mode of intercellular communication, we hypothesized that the payload contained within exosomes is shaped by somatic evolution. To test this, we assayed the impact on primary CD8+ T-cell function, a key mechanism for antitumor immunity, of exosomes derived from three melanoma-related cell lines. While morphologically similar, exosomes from each cell line were functionally different, as B16F0 exosomes dose-dependently suppressed T-cell proliferation. In contrast, Cloudman S91 exosomes promoted T-cell proliferation and Melan-A exosomes had a negligible effect on primary CD8+ T cells. Mechanistically, transcript profiling suggested that exosomal mRNA is enriched for full-length mRNAs that target immune-related pathways. Interestingly, B16F0 exosomes were unique in that they contained both protein and mRNA for PTPN11, which inhibited T-cell proliferation. Collectively, the results suggest that upregulation of PTPN11 by B16F0 exosomes to tumor infiltrating lymphocytes would bypass the extracellular control of the immune checkpoints. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Melanoma exosomes deliver a complex biological payload that upregulates PTPN11 to suppress T lymphocyte function

PubMed Central

Wu, Yueting; Deng, Wentao; McGinley, Emily Chambers; Klinke, David J.

2017-01-01

Summary As exosomes are emerging as a new mode of intercellular communication, we hypothesized that the payload contained within exosomes is shaped by somatic evolution. To test this, we assayed the impact on primary CD8+ T cell function, a key mechanism for anti-tumor immunity, of exosomes derived from three melanoma-related cell lines. While morphologically similar, exosomes from each cell line were functionally different, as B16F0 exosomes dose-dependently suppressed T cell proliferation. In contrast, Cloudman S91 exosomes promoted T cell proliferation and Melan-A exosomes had a negligible effect on primary CD8+ T cells. Mechanistically, transcript profiling suggested that exosomal mRNA is enriched for full-length mRNAs that target immune-related pathways. Interestingly, B16F0 exosomes were unique in that they contained both protein and mRNA for Ptpn11, which inhibited T cell proliferation. Collectively, the results suggest that upregulation of PTPN11 by B16F0 exosomes to tumor infiltrating lymphocytes would bypass the extracellular control of the immune checkpoints. PMID:27930879

49 CFR 91.1 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-10-01

... of the Secretary of Transportation INTERNATIONAL AIR TRANSPORTATION FAIR COMPETITIVE PRACTICES § 91.1... under sections 2 and 3 of the International Air Transportation Fair Competitive Practices Act of 1974 to the end that U.S. flag air carriers operating in foreign air transportation are protected from all...

A kidney injury molecule-1 (Kim-1) gene reporter in a mouse artificial chromosome: the responsiveness to cisplatin toxicity in immortalized mouse kidney S3 cells.

PubMed

Kokura, Kenji; Kuromi, Yasushi; Endo, Takeshi; Anzai, Naohiko; Kazuki, Yasuhiro; Oshimura, Mitsuo; Ohbayashi, Tetsuya

2016-10-01

Kidney injury molecule-1 (Kim-1) has been validated as a urinary biomarker for acute and chronic renal damage. The expression of Kim-1 mRNA is also activated by acute kidney injury induced by cisplatin in rodents and humans. To date, the measurement of Kim-1 expression has not fully allowed the detection of in vitro cisplatin nephrotoxicity in immortalized culture cells, such as human kidney-2 cells and immortalized proximal tubular epithelial cells. We measured the augmentation of Kim-1 mRNA expression after the addition of cisplatin using immortalized S3 cells established from the kidneys of transgenic mice harboring temperature-sensitive large T antigen from Simian virus 40. A mouse Kim-1 gene luciferase reporter in conjunction with an Hprt gene reporter detected cisplatin-induced nephrotoxicity in S3 cells. These two reporter genes were contained in a mouse artificial chromosome, and two luciferases that emitted different wavelengths were used to monitor the respective gene expression. However, the Kim-1 reporter gene failed to respond to cisplatin in A9 fibroblast cells that contained the same reporter mouse artificial chromosome, suggesting cell type-specificity for activation of the reporter. We report the feasibility of measuring in vitro cisplatin nephrotoxicity using a Kim-1 reporter gene in S3 cells. © 2016 The Authors. The Journal of Gene Medicine Published by John Wiley & Sons, Ltd.

40 CFR 91.208 - Certification.

Code of Federal Regulations, 2010 CFR

2010-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 91.208... engines for which certification is requested will not, to the best of the manufacturer's belief, cause the...'s engine families. (2) Declare an FEL for each engine family for HC plus NOX. The FEL must have the...

Defective photoreceptor phagocytosis in a mouse model of enhanced S-cone syndrome causes progressive retinal degeneration

PubMed Central

Mustafi, Debarshi; Kevany, Brian M.; Genoud, Christel; Okano, Kiichiro; Cideciyan, Artur V.; Sumaroka, Alexander; Roman, Alejandro J.; Jacobson, Samuel G.; Engel, Andreas; Adams, Mark D.; Palczewski, Krzysztof

2011-01-01

Enhanced S-cone syndrome (ESCS), featuring an excess number of S cones, manifests as a progressive retinal degeneration that leads to blindness. Here, through optical imaging, we identified an abnormal interface between photoreceptors and the retinal pigment epithelium (RPE) in 9 patients with ESCS. The neural retina leucine zipper transcription factor-knockout (Nrl−/−) mouse model demonstrates many phenotypic features of human ESCS, including unstable S-cone-positive photoreceptors. Using massively parallel RNA sequencing, we identified 6203 differentially expressed transcripts between wild-type (Wt) and Nrl−/− mouse retinas, with 6 highly significant differentially expressed genes of the Pax, Notch, and Wnt canonical pathways. Changes were also obvious in expression of 30 genes involved in the visual cycle and 3 key genes in photoreceptor phagocytosis. Novel high-resolution (100 nm) imaging and reconstruction of Nrl−/− retinas revealed an abnormal packing of photoreceptors that contributed to buildup of photoreceptor deposits. Furthermore, lack of phagosomes in the RPE layer of Nrl−/− retina revealed impairment in phagocytosis. Cultured RPE cells from Wt and Nrl−/− mice illustrated that the phagocytotic defect was attributable to the aberrant interface between ESCS photoreceptors and the RPE. Overcoming the retinal phagocytosis defect could arrest the progressive degenerative component of this disease.—Mustafi, D., Kevany, B. M., Genoud, C., Okano, K., Cideciyan, A. V., Sumaroka, A., Roman, A. J., Jacobson, S. G. Engel, A., Adams, M. D., Palczewski, K. Defective photoreceptor phagocytosis in a mouse model of enhanced S-cone syndrome causes progressive retinal degeneration. PMID:21659555

31 CFR 91.1 - Authority.

Code of Federal Regulations, 2010 CFR

2010-07-01

... and Finance: Treasury Regulations Relating to Money and Finance REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.1 Authority. The regulations in this part governing conduct in and on the Bureau of the Mint buildings and grounds located as follows: U.S. Mint, Colfax, and...

31 CFR 91.2 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Money and Finance: Treasury Regulations Relating to Money and Finance REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.2 Applicability. The regulations in this part apply to the buildings and grounds of the Bureau of the Mint located as follows: U.S. Mint, Colfax and...

40 CFR 91.1309 - Reporting requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Section 91.1309 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Credit Program for New Marine... submitted to: Manager, Engine Compliance Programs Group 6403-J, U.S. Environmental Protection Agency, 1200...

40 CFR 91.603 - Applicability of part 91, subpart F.

Code of Federal Regulations, 2013 CFR

2013-07-01

... violations of the Clean Air Act and the regulations thereunder. (Authorized Company Representative.) (9.... 91.603 Section 91.603 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... representative of the manufacturer: This report is submitted pursuant to Sections 213 and 208 of the Clean Air...

40 CFR 91.603 - Applicability of part 91, subpart F.

Code of Federal Regulations, 2012 CFR

2012-07-01

... violations of the Clean Air Act and the regulations thereunder. (Authorized Company Representative.) (9.... 91.603 Section 91.603 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... representative of the manufacturer: This report is submitted pursuant to Sections 213 and 208 of the Clean Air...

40 CFR 91.603 - Applicability of part 91, subpart F.

Code of Federal Regulations, 2011 CFR

2011-07-01

... violations of the Clean Air Act and the regulations thereunder. (Authorized Company Representative.) (9.... 91.603 Section 91.603 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... representative of the manufacturer: This report is submitted pursuant to Sections 213 and 208 of the Clean Air...

28 CFR 91.10 - General.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Initiative § 91.10 General. (a) Scope of boot camp program. Funding is appropriated in fiscal year 1995 to... Delinquency Prevention Act (42 U.S.C. 5601 et seq.) shall apply. (d) Eligibility. (1) Funding is available for both adult and juvenile boot camps. To be eligible for the funding of boot camps, states must comply...

STS-91 M.S. Franklin Chang-Diaz and Janet Kavandi participate in CEIT

NASA Technical Reports Server (NTRS)

1998-01-01

STS-91 Mission Specialists Franklin Chang-Diaz, Ph.D., and Janet Kavandi, Ph.D., participate in the Crew Equipment Interface Test, or CEIT, in KSC's Orbiter Processing Facility Bay 2. During CEIT, the crew have an opportunity to get a hands-on look at the payloads with which they'll be working on-orbit. The STS-91 crew are scheduled to launch aboard the Shuttle Discovery for the ninth and final docking with the Russian Space Station Mir from KSC's Launch Pad 39A on May 28 at 8:05 EDT.

Use of the mouse jumping test for estimating antagonistic potencies of morphine antagonists.

PubMed

Cowan, A

1976-03-01

The potencies of 19 reference morphine antagonists have been compared in a modified version of the mouse jumping test. Mice were each implanted subcutaneously with one 75 mg pellet of morphine. Antagonist challenge took place 72 h later and the incidence of repetitive vertical-jumping was monitored over 1 h. A high Pearson correlation coefficient (r = 0.997) was found between quantitative assays based on the total number of jumps per mouse and quantal assays based on mice jumping at least 6 times. A comparison of relative potencies obtained with the mouse test and with non-withdrawn morphine-dependent monkeys gave a Spearman rank order coefficient of 0.91 while a similar comparison with values obtained with the guinea-pig isolated ileum preparation also gave a high correlation coefficient (r= 0.92). Whereas it is difficult to assess the antagonistic component of buprenorphine and cyclorphan with the ileum preparation, both compounds can be satisfactorily assayed in the mouse jumping test. The reported antagonistic properties of ketocyclazocine and profadol could not be confirmed in the mouse model.

A Mouse Geneticist’s Practical Guide to CRISPR Applications

PubMed Central

Singh, Priti; Schimenti, John C.; Bolcun-Filas, Ewelina

2015-01-01

CRISPR/Cas9 system of RNA-guided genome editing is revolutionizing genetics research in a wide spectrum of organisms. Even for the laboratory mouse, a model that has thrived under the benefits of embryonic stem (ES) cell knockout capabilities for nearly three decades, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 technology enables one to manipulate the genome with unprecedented simplicity and speed. It allows generation of null, conditional, precisely mutated, reporter, or tagged alleles in mice. Moreover, it holds promise for other applications beyond genome editing. The crux of this system is the efficient and targeted introduction of DNA breaks that are repaired by any of several pathways in a predictable but not entirely controllable manner. Thus, further optimizations and improvements are being developed. Here, we summarize current applications and provide a practical guide to use the CRISPR/Cas9 system for mouse mutagenesis, based on published reports and our own experiences. We discuss critical points and suggest technical improvements to increase efficiency of RNA-guided genome editing in mouse embryos and address practical problems such as mosaicism in founders, which complicates genotyping and phenotyping. We describe a next-generation sequencing strategy for simultaneous characterization of on- and off-target editing in mice derived from multiple CRISPR experiments. Additionally, we report evidence that elevated frequency of precise, homology-directed editing can be achieved by transient inhibition of the Ligase IV-dependent nonhomologous end-joining pathway in one-celled mouse embryos. PMID:25271304

14 CFR 91.1049 - Personnel.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Program manager flight, duty, and rest time considerations, and in all cases within the limits set forth in §§ 91.1057 through 91.1061. (3) Vacations. (4) Operational efficiencies. (5) Training. (6) Single... flight, duty, and rest time limits in §§ 91.1057 through 91.1061 in program operations. (d) Unless...

A prior feature SVM – MRF based method for mouse brain segmentation

PubMed Central

Wu, Teresa; Bae, Min Hyeok; Zhang, Min; Pan, Rong; Badea, Alexandra

2012-01-01

We introduce an automated method, called prior feature Support Vector Machine- Markov Random Field (pSVMRF), to segment three-dimensional mouse brain Magnetic Resonance Microscopy (MRM) images. Our earlier work, extended MRF (eMRF) integrated Support Vector Machine (SVM) and Markov Random Field (MRF) approaches, leading to improved segmentation accuracy; however, the computation of eMRF is very expensive, which may limit its performance on segmentation and robustness. In this study pSVMRF reduces training and testing time for SVM, while boosting segmentation performance. Unlike the eMRF approach, where MR intensity information and location priors are linearly combined, pSVMRF combines this information in a nonlinear fashion, and enhances the discriminative ability of the algorithm. We validate the proposed method using MR imaging of unstained and actively stained mouse brain specimens, and compare segmentation accuracy with two existing methods: eMRF and MRF. C57BL/6 mice are used for training and testing, using cross validation. For formalin fixed C57BL/6 specimens, pSVMRF outperforms both eMRF and MRF. The segmentation accuracy for C57BL/6 brains, stained or not, was similar for larger structures like hippocampus and caudate putamen, (~87%), but increased substantially for smaller regions like susbtantia nigra (from 78.36% to 91.55%), and anterior commissure (from ~50% to ~80%). To test segmentation robustness against increased anatomical variability we add two strains, BXD29 and a transgenic mouse model of Alzheimer’s Disease. Segmentation accuracy for new strains is 80% for hippocampus, and caudate putamen, indicating that pSVMRF is a promising approach for phenotyping mouse models of human brain disorders. PMID:21988893

40 CFR 91.801 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Testing and Recall Regulations § 91.801 Applicability... repairs. (f) The requirements of the Manufacturer In-use testing program set forth in §§ 91.803 through 91.805 are waived for existing technology OB/PWC as defined in § 91.3 through model year 2003. (1) The...

Long-term strength and allowable stresses of grade 10Kh9MFB and X10CrMoVNb9-1 (T91/P91) chromium heat-resistant steels

NASA Astrophysics Data System (ADS)

Skorobogatykh, V. N.; Danyushevskiy, I. A.; Schenkova, I. A.; Prudnikov, D. A.

2015-04-01

Currently, grade X10CrMoVNb9-1 (T91, P91) and 10Kh9MFB (10Kh9MFB-Sh) chromium steels are widely applied in equipment manufacturing for thermal power plants in Russia and abroad. Compilation and comparison of tensile, impact, and long-term strength tests results accumulated for many years of investigations of foreign grade X10CrMoVNb9-1, T91, P91, and domestic grade 10Kh9MFB (10Kh9MFB-Sh) steels is carried out. The property identity of metals investigated is established. High strength and plastic properties of steels, from which pipes and other products are made, for operation under creep conditions are confirmed. Design characteristics of long-term strength on the basis of tests with more than one million of hour-samples are determined ( and at temperatures of 500-650°C). The table of recommended allowable stresses for grade 10Kh9MFB, 10Kh9MFB-SH, X10CrMoVNb9-1, T91, and P91 steels is developed. The long-time properties of pipe welded joints of grade 10Kh9MFB+10Kh9MFB, 10Kh9MFB-Sh+10Kh9MFB-Sh, X10CrMoVNb9-1+X10CrMoVNb9-1, P91+P91, T91+T91, 10Kh9MFB (10Kh9MFB-Sh)+X10CrMoVNb9-1(T/P91) steels is researched. The welded joint reduction factor is experimentally determined.

Transport of N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine, a metabolite of trichloroethylene, by mouse multidrug resistance associated protein 2 (Mrp2)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tsirulnikov, Kirill; Abuladze, Natalia; Koag, Myong-Chul

2010-04-15

N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine (Ac-DCVC) and S-(1,2-dichlorovinyl)-L-cysteine (DCVC) are the glutathione conjugation pathway metabolites of a common industrial contaminant and potent nephrotoxicant trichloroethylene (TCE). Ac-DCVC and DCVC are accumulated in the renal proximal tubule where they may be secreted into the urine by an unknown apical transporter(s). In this study, we explored the hypothesis that the apical transport of Ac-DCVC and/or DCVC may be mediated by the multidrug resistance associated protein 2 (Mrp2, ABCC2), which is known to mediate proximal tubular apical ATP-dependent transport of glutathione and numerous xenobiotics and endogenous substances conjugated with glutathione. Transport experiments using membrane vesicles prepared from mousemore » proximal tubule derived cells expressing mouse Mrp2 utilizing ATPase assay and direct measurements of Ac-DCVC/DCVC using liquid chromatography/tandem mass-spectrometry (LC/MS/MS) demonstrated that mouse Mrp2 mediates ATP-dependent transport of Ac-DCVC. Expression of mouse Mrp2 antisense mRNA significantly inhibited the vectorial basolateral to apical transport of Ac-DCVC but not DCVC in mouse proximal tubule derived cells endogenously expressing mouse Mrp2. The results suggest that Mrp2 may be involved in the renal secretion of Ac-DCVC.« less

On the intracellular trafficking of mouse S5 ribosomal protein from cytoplasm to nucleoli.

PubMed

Matragkou, Ch; Papachristou, H; Karetsou, Z; Papadopoulos, G; Papamarcaki, T; Vizirianakis, I S; Tsiftsoglou, A S; Choli-Papadopoulou, T

2009-10-09

The non-ribosomal functions of mammalian ribosomal proteins have recently attracted worldwide attention. The mouse ribosomal protein S5 (rpS5) derived from ribosomal material is an assembled non-phosphorylated protein. The free form of rpS5 protein, however, undergoes phosphorylation. In this study, we have (a) investigated the potential role of phosphorylation in rpS5 protein transport into the nucleus and then into nucleoli and (b) determined which of the domains of rpS5 are involved in this intracellular trafficking. In vitro PCR mutagenesis of mouse rpS5 cDNA, complemented by subsequent cloning and expression of rpS5 truncated recombinant forms, produced in fusion with green fluorescent protein, permitted the investigation of rpS5 intracellular trafficking in HeLa cells using confocal microscopy complemented by Western blot analysis. Our results indicate the following: (a) rpS5 protein enters the nucleus via the region 38-50 aa that forms a random coil as revealed by molecular dynamic simulation. (b) Immunoprecipitation of rpS5 with casein kinase II and immobilized metal affinity chromatography analysis complemented by in vitro kinase assay revealed that phosphorylation of rpS5 seems to be indispensable for its transport from nucleus to nucleoli; upon entering the nucleus, Thr-133 phosphorylation triggers Ser-24 phosphorylation by casein kinase II, thus promoting entrance of rpS5 into the nucleoli. Another important role of rpS5 N-terminal region is proposed to be the regulation of protein's cellular level. The repetitively co-appearance of a satellite C-terminal band below the entire rpS5 at the late stationary phase, and not at the early logarithmic phase, of cell growth suggests a specific degradation balancing probably the unassembled ribosomal protein molecules with those that are efficiently assembled to ribosomal subunits. Overall, these data provide new insights on the structural and functional domains within the rpS5 molecule that contribute to its

Identification and chromosomal localization of Atm, the mouse homolog of the ataxia-telangiectasia gene.

PubMed

Pecker, I; Avraham, K B; Gilbert, D J; Savitsky, K; Rotman, G; Harnik, R; Fukao, T; Schröck, E; Hirotsune, S; Tagle, D A; Collins, F S; Wynshaw-Boris, A; Ried, T; Copeland, N G; Jenkins, N A; Shiloh, Y; Ziv, Y

1996-07-01

Atm, the mouse homolog of the human ATM gene defective in ataxia-telangiectasia (A-T), has been identified. The entire coding sequence of the Atm transcript was cloned and found to contain an open reading frame encoding a protein of 3066 amino acids with 84% overall identity and 91% similarity to the human ATM protein. Variable levels of expression of Atm were observed in different tissues. Fluorescence in situ hybridization and linkage analysis located the Atm gene on mouse chromosome 9, band 9C, in a region homologous to the ATM region on human chromosome 11q22-q23.

NLC-91

NASA Technical Reports Server (NTRS)

Kopp, E.; Witt, G.; Goldberg, R. A.

1991-01-01

Noctilucent Clouds (NLC)-91 is a multinational rocket and radar program which will take place in Jul. - Aug. 1991 in northern Scandinavia and the Barents Sea. The main objective of the campaign is to determine with in situ experiments the dynamical, electrodynamical, physical, and chemical parameters of an NLC layer combined with ground based visible radar, lidar, and microwave experiments. The altitude resolution of ground based and in situ measurements in the cold mesopause region should be improved in NLC-91 compared to the previous campaigns below 100 m.

Stereoselective formation of a cholesterol ester conjugate from fenvalerate by mouse microsomal carboxyesterase(s).

PubMed

Miyamoto, J; Kaneko, H; Takamatsu, Y

1986-06-01

In accordance with in vivo findings, of the four chiral isomers of fenvalerate (S-5602 Sumicidin, Pydrin, [RS]-alpha-cyano-3-phenoxybenzyl [RS]-2-(4-chlorophenyl)isovalerate), only the [2R, alpha S]-isomer (B-isomer) yielded cholesteryl [2R]-2-(4-chlorophenyl)isovalerate (CPIA-cholesterol ester) in the in vitro study using several tissue homogenates of mice, rats, dogs, and monkeys. There were species differences in the extent of CPIA-cholesterol-ester formation, with mouse tissues showing relatively higher activity than those of other animals. The kidney, brain, and spleen of mice showed relatively higher capacities to form this ester compared to other tissues, and the enzyme activity was mainly localized in microsomal fractions. The CPIA-cholesterol ester did not seem to be produced by three known biosynthetic pathways of endogenous cholesterol esters--acyl-CoA:cholesterol O-acyltransferase (ACAT), lecithin:cholesterol O-acyltransferase (LCAT), and cholesterol esterase. Carboxyesterase(s) of mouse kidney microsomes solubilized by digitonin hydrolyzed only the B alpha-isomer of fenvalerate, yielding CPIA, whereas they yielded the corresponding cholesterol ester in the presence of artificial liposomes containing cholesterol. Thus, it appears that the stereoselective formation of the CPIA-cholesterol ester results from the stereoselective formation of the CPIA-carboxyesterase complex only from the B alpha-isomer, which subsequently undergoes cleavage by cholesterol to yield the CPIA-cholesterol ester.

Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)α-hydroxysteroid dehydrogenase

DOE Office of Scientific and Technical Information (OSTI.GOV)

El-Kabbani, Ossama, E-mail: ossama.el-kabbani@vcp.monash.edu.au; Ishikura, Syuhei; Wagner, Armin

2005-07-01

Orthorhombic crystals of mouse 3(17)α-hydroxysteroid dehydrogenase were obtained from buffered polyethylene glycol solutions. The crystals diffracted to a resolution of 1.8 Å at the Swiss Light Source beamline X06SA. The 3(17)α-hydroxysteroid dehydrogenase from mouse is involved in the metabolism of oestrogens, androgens, neurosteroids and xenobiotic compounds. The enzyme was crystallized by the hanging-drop vapour-diffusion method in space group P222{sub 1}, with unit-cell parameters a = 84.91, b = 84.90, c = 95.83 Å. The Matthews coefficient (V{sub M}) and the solvent content were 2.21 Å{sup 3} Da{sup −1} and 44.6%, respectively, assuming the presence of two molecules in the asymmetricmore » unit. Diffraction data were collected to a resolution of 1.8 Å at the Swiss Light Source beamline X06SA using a MAR CCD area detector and gave a data set with an overall R{sub merge} of 6.8% and a completeness of 91.1%.« less

Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages

PubMed Central

Deng, Tingting; Zhang, Yue; Chen, Qiaoyuan; Yan, Keqin; Han, Daishu

2012-01-01

Activation of Toll-like receptors (TLRs) triggers rapid inflammatory cytokine production in various cell types. The exogenous product of growth-arrest-specific gene 6 (Gas6) and Protein S (ProS) inhibit the TLR-triggered inflammatory responses through the activation of Tyro3, Axl and Mer (TAM) receptors. However, regulation of the Gas6/ProS-TAM system remains largely unknown. In the current study, mouse macrophages are shown to constitutively express Gas6 and ProS, which synergistically suppress the basal and TLR-triggered production of inflammatory cytokines, including those of tumour necrosis factor-α, interleukin-6 and interleukin-1β, by the macrophages in an autocrine manner. Notably, TLR signalling markedly decreases Gas6 and ProS expression in macrophages through the activation of the nuclear factor-κB. Further, the down-regulation of Gas6 and ProS by TLR signalling facilitates the TLR-mediated inflammatory cytokine production in mouse macrophages. These results describe a self-regulatory mechanism of TLR signalling through the suppression of Gas6 and ProS expression. PMID:22043818

40 CFR 440.91 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 30 2011-07-01 2011-07-01 false [Reserved] 440.91 Section 440.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.91 [Reserved] ...

40 CFR 440.91 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 29 2010-07-01 2010-07-01 false [Reserved] 440.91 Section 440.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.91 [Reserved] ...

40 CFR 91.601 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Applicability. 91.601 Section 91.601 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Selective Enforcement Auditing Regulations § 91.601...

Galaxy interactions in compact groups - II. Abundance and kinematic anomalies in HCG 91c

NASA Astrophysics Data System (ADS)

Vogt, Frédéric P. A.; Dopita, Michael A.; Borthakur, Sanchayeeta; Verdes-Montenegro, Lourdes; Heckman, Timothy M.; Yun, Min S.; Chambers, Kenneth C.

2015-07-01

Galaxies in Hickson Compact Group 91 (HCG 91) were observed with the WiFeS integral field spectrograph as part of our ongoing campaign targeting the ionized gas physics and kinematics inside star-forming members of compact groups. Here, we report the discovery of H II regions with abundance and kinematic offsets in the otherwise unremarkable star-forming spiral HCG 91c. The optical emission line analysis of this galaxy reveals that at least three H II regions harbour an oxygen abundance ˜0.15 dex lower than expected from their immediate surroundings and from the abundance gradient present in the inner regions of HCG 91c. The same star-forming regions are also associated with a small kinematic offset in the form of a lag of 5-10 km s-1 with respect to the local circular rotation of the gas. H I observations of HCG 91 from the Very Large Array and broad-band optical images from Pan-STARRS (Panoramic Survey Telescope And Rapid Response System) suggest that HCG 91c is caught early in its interaction with the other members of HCG 91. We discuss different scenarios to explain the origin of the peculiar star-forming regions detected with WiFeS, and show that evidence points towards infalling and collapsing extraplanar gas clouds at the disc-halo interface, possibly as a consequence of long-range gravitational perturbations of HCG 91c from the other group members. As such, HCG 91c provides evidence that some of the perturbations possibly associated with the early phase of galaxy evolution in compact groups impact the star-forming disc locally, and on sub-kpc scales.

Changes in voiding behavior in a mouse model of Alzheimer’s disease

PubMed Central

Biallosterski, B. T.; Prickaerts, J.; Rahnama’i, M. S.; de Wachter, S.; van Koeveringe, G. A.; Meriaux, C.

2015-01-01

Besides cognitive decline and behavioral alteration, urinary incontinence often occurs in patients suffering from Alzheimer’s disease (AD). To determine whether the transgenic mouse model of AD, APP/PS1 (APPSL/PS1M146L) mouse, shows alteration of the urinary bladder function and anxiety, as for patients with AD, we examined the urinary marking behavior in relation to affective behavior. At 18 months of age voiding behavior of APP/PS1 and wild type (WT) mice was assessed by using a modified filter paper assay in combination with video tracing, with the cage divided into a center and corner zones. Anxiety-related behavior and locomotion were respectively tested in an elevated zero maze (EZM) and an open field (OF). The APP/PS1 mice urinated more in the center zone than the WT mice. The total volume of markings was significantly lower in the APP/PS1 mice. In both groups, the average volume of a marking in the corner zone was larger than in the center zone. In the EZM, the APP/PS1 mice spent less time in the open arms of the arena, considered as anxiogenic zones, than the WT mice. During the OF task, the APP/PS1 mice covered a longer distance than the WT mice. These findings show that the APP/PS1 mice have a different voiding behavior compared to the WT mice, i.e., urinating with small volumes and voiding in the center of the cage, and suggest that increased locomotor activity and anxiety-related behaviors are factors in the change in voiding pattern in the APP/PS1 mouse. PMID:26379542

47 CFR 9.1 - Purposes.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 1 2013-10-01 2013-10-01 false Purposes. 9.1 Section 9.1 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL INTERCONNECTED VOICE OVER INTERNET PROTOCOL SERVICES § 9.1... applicable to interconnected Voice over Internet Protocol service providers, and to ensure that those...

47 CFR 9.1 - Purposes.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 1 2014-10-01 2014-10-01 false Purposes. 9.1 Section 9.1 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL INTERCONNECTED VOICE OVER INTERNET PROTOCOL SERVICES § 9.1... applicable to interconnected Voice over Internet Protocol service providers, and to ensure that those...

47 CFR 9.1 - Purposes.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 1 2012-10-01 2012-10-01 false Purposes. 9.1 Section 9.1 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL INTERCONNECTED VOICE OVER INTERNET PROTOCOL SERVICES § 9.1... applicable to interconnected Voice over Internet Protocol service providers, and to ensure that those...

47 CFR 9.1 - Purposes.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 1 2011-10-01 2011-10-01 false Purposes. 9.1 Section 9.1 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL INTERCONNECTED VOICE OVER INTERNET PROTOCOL SERVICES § 9.1... applicable to interconnected Voice over Internet Protocol service providers, and to ensure that those...

47 CFR 9.1 - Purposes.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 1 2010-10-01 2010-10-01 false Purposes. 9.1 Section 9.1 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL INTERCONNECTED VOICE OVER INTERNET PROTOCOL SERVICES § 9.1... applicable to interconnected Voice over Internet Protocol service providers, and to ensure that those...

40 CFR 440.91 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 30 2014-07-01 2014-07-01 false [Reserved] 440.91 Section 440.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.91 [Reserved] ...

40 CFR 440.91 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 31 2013-07-01 2013-07-01 false [Reserved] 440.91 Section 440.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.91 [Reserved] ...

40 CFR 440.91 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 31 2012-07-01 2012-07-01 false [Reserved] 440.91 Section 440.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) ORE MINING AND DRESSING POINT SOURCE CATEGORY Antimony Ore Subcategory § 440.91 [Reserved] ...

7 CFR 91.3 - Authority.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false Authority. 91.3 Section 91.3 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND GENERAL INFORMATION Administration § 91.3 Authority. The Deputy Administrator is charged with the...

40 CFR 91.602 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Definitions. 91.602 Section 91.602 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Selective Enforcement Auditing Regulations § 91.602 Definitions...

29 CFR 1915.91 - Housekeeping.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 7 2010-07-01 2010-07-01 false Housekeeping. 1915.91 Section 1915.91 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) OCCUPATIONAL SAFETY AND HEALTH STANDARDS FOR SHIPYARD EMPLOYMENT General Working Conditions § 1915.91...

7 CFR 3570.91 - Regulations.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 15 2011-01-01 2011-01-01 false Regulations. 3570.91 Section 3570.91 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE COMMUNITY PROGRAMS Community Facilities Grant Program § 3570.91 Regulations. Grants under this part will be...

7 CFR 3570.91 - Regulations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Regulations. 3570.91 Section 3570.91 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE COMMUNITY PROGRAMS Community Facilities Grant Program § 3570.91 Regulations. Grants under this part will be...

50 CFR 91.2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Definitions. 91.2 Section 91.2 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MISCELLANEOUS PROVISIONS MIGRATORY BIRD HUNTING AND CONSERVATION STAMP CONTEST Introduction § 91.2 Definitions...

50 CFR 91.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false Definitions. 91.2 Section 91.2 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MISCELLANEOUS PROVISIONS MIGRATORY BIRD HUNTING AND CONSERVATION STAMP CONTEST Introduction § 91.2 Definitions...

24 CFR 91.20 - Exceptions.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Exceptions. 91.20 Section 91.20 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS General § 91.20 Exceptions. The HUD...

24 CFR 91.2 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Applicability. 91.2 Section 91.2 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS General § 91.2 Applicability. (a) The...

24 CFR 91.330 - Monitoring.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Monitoring. 91.330 Section 91.330 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development... Consolidated Plan § 91.330 Monitoring. The consolidated plan must describe the standards and procedures that...

24 CFR 91.430 - Monitoring.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Monitoring. 91.430 Section 91.430 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development... Plan § 91.430 Monitoring. The consolidated plan must describe the standards and procedures that the...

Monte Carlo-based evaluation of S-values in mouse models for positron-emitting radionuclides

NASA Astrophysics Data System (ADS)

Xie, Tianwu; Zaidi, Habib

2013-01-01

In addition to being a powerful clinical tool, Positron emission tomography (PET) is also used in small laboratory animal research to visualize and track certain molecular processes associated with diseases such as cancer, heart disease and neurological disorders in living small animal models of disease. However, dosimetric characteristics in small animal PET imaging are usually overlooked, though the radiation dose may not be negligible. In this work, we constructed 17 mouse models of different body mass and size based on the realistic four-dimensional MOBY mouse model. Particle (photons, electrons and positrons) transport using the Monte Carlo method was performed to calculate the absorbed fractions and S-values for eight positron-emitting radionuclides (C-11, N-13, O-15, F-18, Cu-64, Ga-68, Y-86 and I-124). Among these radionuclides, O-15 emits positrons with high energy and frequency and produces the highest self-absorbed S-values in each organ, while Y-86 emits γ-rays with high energy and frequency which results in the highest cross-absorbed S-values for non-neighbouring organs. Differences between S-values for self-irradiated organs were between 2% and 3%/g difference in body weight for most organs. For organs irradiating other organs outside the splanchnocoele (i.e. brain, testis and bladder), differences between S-values were lower than 1%/g. These appealing results can be used to assess variations in small animal dosimetry as a function of total-body mass. The generated database of S-values for various radionuclides can be used in the assessment of radiation dose to mice from different radiotracers in small animal PET experiments, thus offering quantitative figures for comparative dosimetry research in small animal models.

45 CFR 9.1 - Purpose.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Purpose. 9.1 Section 9.1 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION USE OF HHS RESEARCH FACILITIES BY ACADEMIC SCIENTISTS, ENGINEERS, AND STUDENTS § 9.1 Purpose. To enhance the availability of DHHS scientific research...

45 CFR 9.1 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Purpose. 9.1 Section 9.1 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION USE OF HHS RESEARCH FACILITIES BY ACADEMIC SCIENTISTS, ENGINEERS, AND STUDENTS § 9.1 Purpose. To enhance the availability of DHHS scientific research...

40 CFR 61.91 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... included. The method for calculating effective dose equivalent and the definition of reference man are... 40 Protection of Environment 8 2010-07-01 2010-07-01 false Definitions. 61.91 Section 61.91... Other Than Radon From Department of Energy Facilities § 61.91 Definitions. As used in this subpart, all...

40 CFR 91.1306 - Trading.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Trading. 91.1306 Section 91.1306... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Credit Program for New Marine Engines § 91.1306 Trading... engine manufacturers through trading. (b) In-use credits for trading can be obtained from credits banked...

40 CFR 91.206 - Trading.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Trading. 91.206 Section 91.206... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 91.206 Trading. (a... manufacturers in trading. These credits must be used in the same averaging set as generated. (b) Credits for...

10 CFR 36.91 - Violations.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 1 2011-01-01 2011-01-01 false Violations. 36.91 Section 36.91 Energy NUCLEAR REGULATORY COMMISSION LICENSES AND RADIATION SAFETY REQUIREMENTS FOR IRRADIATORS Enforcement § 36.91 Violations. (a) The Commission may obtain an injunction or other court order to prevent a violation of the provisions of— (1) The...

10 CFR 36.91 - Violations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Violations. 36.91 Section 36.91 Energy NUCLEAR REGULATORY COMMISSION LICENSES AND RADIATION SAFETY REQUIREMENTS FOR IRRADIATORS Enforcement § 36.91 Violations. (a) The Commission may obtain an injunction or other court order to prevent a violation of the provisions of— (1) The...

31 CFR 91.10 - Photographs.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Photographs. 91.10 Section 91.10 Money and Finance: Treasury Regulations Relating to Money and Finance REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.10 Photographs. The taking of photographs on...

7 CFR 91.101 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false Definitions. 91.101 Section 91.101 Agriculture... Officially Tested By AMS § 91.101 Definitions. Words used in the regulations in this part in the singular... United States of America to another country in the course of trade. Laboratory. An AMS Science and...

40 CFR 91.1306 - Trading.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 21 2012-07-01 2012-07-01 false Trading. 91.1306 Section 91.1306... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Credit Program for New Marine Engines § 91.1306 Trading... engine manufacturers through trading. (b) In-use credits for trading can be obtained from credits banked...

40 CFR 91.206 - Trading.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Trading. 91.206 Section 91.206... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 91.206 Trading. (a... manufacturers in trading. These credits must be used in the same averaging set as generated. (b) Credits for...

40 CFR 91.1306 - Trading.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Trading. 91.1306 Section 91.1306... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Credit Program for New Marine Engines § 91.1306 Trading... engine manufacturers through trading. (b) In-use credits for trading can be obtained from credits banked...

40 CFR 91.206 - Trading.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Trading. 91.206 Section 91.206... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 91.206 Trading. (a... manufacturers in trading. These credits must be used in the same averaging set as generated. (b) Credits for...

40 CFR 91.206 - Trading.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 21 2012-07-01 2012-07-01 false Trading. 91.206 Section 91.206... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 91.206 Trading. (a... manufacturers in trading. These credits must be used in the same averaging set as generated. (b) Credits for...

40 CFR 91.1306 - Trading.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Trading. 91.1306 Section 91.1306... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Credit Program for New Marine Engines § 91.1306 Trading... engine manufacturers through trading. (b) In-use credits for trading can be obtained from credits banked...

40 CFR 91.1306 - Trading.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Trading. 91.1306 Section 91.1306... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Credit Program for New Marine Engines § 91.1306 Trading... engine manufacturers through trading. (b) In-use credits for trading can be obtained from credits banked...

40 CFR 91.206 - Trading.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Trading. 91.206 Section 91.206... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Averaging, Banking, and Trading Provisions § 91.206 Trading. (a... manufacturers in trading. These credits must be used in the same averaging set as generated. (b) Credits for...

Job Language Performance Requirements for MOS 91B, Medical Specialist, Reference Soldier’s Manual Dated 30 August 1977.

DTIC Science & Technology

1977-08-30

Administer tube feeding (gavage) to a patient. 081-91B-1238 Administer a rectal suppository . 081-91B-1240 Administer an intramuscular injection. 081-91B...communications Coded messages Spellings Conversation Requests VI-3 * * * SPEAKING TASK: Formulate and produce appropriate oral responses spontaneously

9 CFR 91.6 - Goats.

Code of Federal Regulations, 2010 CFR

2010-01-01

... prescribed in Veterinary Services Memorandum 552.15. (2) Brucellosis. Dairy and breeding goats shall be... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Goats. 91.6 Section 91.6 Animals and... EXPORTATION Diagnostic Tests, Treatments § 91.6 Goats. (a) In order to be eligible for export, goats shall be...

9 CFR 91.6 - Goats.

Code of Federal Regulations, 2011 CFR

2011-01-01

... prescribed in Veterinary Services Memorandum 552.15. (2) Brucellosis. Dairy and breeding goats shall be... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Goats. 91.6 Section 91.6 Animals and... EXPORTATION Diagnostic Tests, Treatments § 91.6 Goats. (a) In order to be eligible for export, goats shall be...

45 CFR 91.43 - Mediation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Mediation. 91.43 Section 91.43 Public Welfare... Enforcement Procedures § 91.43 Mediation. (a) HHS will promptly refer to a mediation agency designated by the... mediation process to the extent necessary to reach an agreement or make an informed judgment that an...

31 CFR 91.7 - Gambling.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Gambling. 91.7 Section 91.7 Money and Finance: Treasury Regulations Relating to Money and Finance REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.7 Gambling. (a) Participating in games for money or other...

31 CFR 91.6 - Nuisances.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Nuisances. 91.6 Section 91.6 Money and Finance: Treasury Regulations Relating to Money and Finance REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.6 Nuisances. The use of loud, abusive, or profane...

Sensorimotor assessment of the unilateral 6-hydroxydopamine mouse model of Parkinson’s disease

PubMed Central

Glajch, Kelly E.; Fleming, Sheila M.; Surmeier, D. James; Osten, Pavel

2012-01-01

Parkinson’s disease (PD), the second most common neurodegenerative disorder, is characterized by marked impairments in motor function caused by the progressive loss of dopaminergic neurons in the substantia nigra pars compacta (SNc). Animal models of PD have traditionally been based on toxins, such as 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), that selectively lesion dopaminergic neurons. Motor impairments from 6-OHDA lesions of SNc neurons are well characterized in rats, but much less work has been done in mice. In this study, we compare the effectiveness of a series of drug-free behavioral tests in assessing sensorimotor impairments in the unilateral 6-OHDA mouse model, including six tests used for the first time in this PD mouse model (the automated treadmill “DigiGait” test, the challenging beam test, the adhesive removal test, the pole test, the adjusting steps test, and the test of spontaneous activity) and two tests used previously in 6-OHDA-lesioned mice (the limb-use asymmetry “cylinder” test and the manual gait test). We demonstrate that the limb-use asymmetry, challenging beam, pole, adjusting steps, and spontaneous activity tests are all highly robust assays for detecting sensorimotor impairments in the 6-OHDA mouse model. We also discuss the use of the behavioral tests for specific experimental objectives, such as simple screening for well-lesioned mice in studies of PD cellular pathophysiology or comprehensive behavioral analysis in preclinical therapeutic testing using a battery of sensorimotor tests. PMID:22178078

Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91.

PubMed

Wladyka, Benedykt; Wielebska, Katarzyna; Wloka, Marcin; Bochenska, Oliwia; Dubin, Grzegorz; Dubin, Adam; Mak, Pawel

2013-08-01

Staphylococcus aureus strain CH-91, isolated from a broiler chicken with atopic dermatitis, has a highly proteolytic phenotype that is correlated with the disease. We describe the isolation and biochemical and molecular characterization of the AI-type lantibiotic BacCH91 from S. aureus CH-91 culture medium. The bacteriocin was purified using a three-stage procedure comprising precipitation with ammonium sulfate, extraction with organic solvents, and reversed-phase HPLC. The BacCH91 peptide is thermostable and highly resistant to cleavage by both prokaryotic and eukaryotic peptidases. The MIC for the Gram-positive bacteria ranged from 2.5 nM for Microococcus luteus through 1.3-6.0 μM for staphylococcal strains up to more than 100 μM for Lactococcus lactis. BacCH91 was ineffective against the Gram-negative strains tested at the maximal concentration (100 μM). The amino acid sequence of BacCH91 is similar to that of epidermin and gallidermin. The encoding gene (bacCH91) occurred in two allelic variants distinguishable in the restriction fragment length polymorphism assay. Variant I, identified in S. aureus CH-91, dominated in S. aureus strains of poultry origin, although strains with variant II were also identified in this group. S. aureus strains of human origin were characterized exclusively by variant II.

The Mouse Genome Database (MGD): facilitating mouse as a model for human biology and disease.

PubMed

Eppig, Janan T; Blake, Judith A; Bult, Carol J; Kadin, James A; Richardson, Joel E

2015-01-01

The Mouse Genome Database (MGD, http://www.informatics.jax.org) serves the international biomedical research community as the central resource for integrated genomic, genetic and biological data on the laboratory mouse. To facilitate use of mouse as a model in translational studies, MGD maintains a core of high-quality curated data and integrates experimentally and computationally generated data sets. MGD maintains a unified catalog of genes and genome features, including functional RNAs, QTL and phenotypic loci. MGD curates and provides functional and phenotype annotations for mouse genes using the Gene Ontology and Mammalian Phenotype Ontology. MGD integrates phenotype data and associates mouse genotypes to human diseases, providing critical mouse-human relationships and access to repositories holding mouse models. MGD is the authoritative source of nomenclature for genes, genome features, alleles and strains following guidelines of the International Committee on Standardized Genetic Nomenclature for Mice. A new addition to MGD, the Human-Mouse: Disease Connection, allows users to explore gene-phenotype-disease relationships between human and mouse. MGD has also updated search paradigms for phenotypic allele attributes, incorporated incidental mutation data, added a module for display and exploration of genes and microRNA interactions and adopted the JBrowse genome browser. MGD resources are freely available to the scientific community. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

9 CFR 91.29 - Hatches.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

9 CFR 91.29 - Hatches.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

9 CFR 91.29 - Hatches.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

9 CFR 91.29 - Hatches.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

9 CFR 91.9 - Swine.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Swine. 91.9 Section 91.9 Animals and... EXPORTATION Diagnostic Tests, Treatments § 91.9 Swine. (a) No swine shall be exported if they were fed garbage at any time. The swine shall be accompanied by a certification from the owner stating that they were...

9 CFR 91.9 - Swine.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Swine. 91.9 Section 91.9 Animals and... EXPORTATION Diagnostic Tests, Treatments § 91.9 Swine. (a) No swine shall be exported if they were fed garbage at any time. The swine shall be accompanied by a certification from the owner stating that they were...

9 CFR 91.9 - Swine.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Swine. 91.9 Section 91.9 Animals and... EXPORTATION Diagnostic Tests, Treatments § 91.9 Swine. (a) No swine shall be exported if they were fed garbage at any time. The swine shall be accompanied by a certification from the owner stating that they were...

9 CFR 91.9 - Swine.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Swine. 91.9 Section 91.9 Animals and... EXPORTATION Diagnostic Tests, Treatments § 91.9 Swine. (a) No swine shall be exported if they were fed garbage at any time. The swine shall be accompanied by a certification from the owner stating that they were...

31 CFR 91.7 - Gambling.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Gambling. 91.7 Section 91.7 Money and... REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.7 Gambling. (a) Participating in games for money or other property, the operation of gambling devices, the conduct of a lottery...

31 CFR 91.7 - Gambling.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Gambling. 91.7 Section 91.7 Money and... REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.7 Gambling. (a) Participating in games for money or other property, the operation of gambling devices, the conduct of a lottery...

31 CFR 91.7 - Gambling.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Gambling. 91.7 Section 91.7 Money and... REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.7 Gambling. (a) Participating in games for money or other property, the operation of gambling devices, the conduct of a lottery...

Job Language Performance Requirements for MOS 91C, Clinical Specialist, Reference Soldier’s Manual Dated 30 August 1977.

DTIC Science & Technology

1977-08-30

Administer a rectal suppository 081-91C-1240 Administer an injection (SC or IM) 081-91C-1241 Administer an intradermal injection 081-91C-1242 Administer a...in this language task: Shouting Radio communications Coded messages Spellings Conversation Requests wI-3 SPEAKING *TASK: Formulate and produce

Mouse Tumor Biology (MTB): a database of mouse models for human cancer.

PubMed

Bult, Carol J; Krupke, Debra M; Begley, Dale A; Richardson, Joel E; Neuhauser, Steven B; Sundberg, John P; Eppig, Janan T

2015-01-01

The Mouse Tumor Biology (MTB; http://tumor.informatics.jax.org) database is a unique online compendium of mouse models for human cancer. MTB provides online access to expertly curated information on diverse mouse models for human cancer and interfaces for searching and visualizing data associated with these models. The information in MTB is designed to facilitate the selection of strains for cancer research and is a platform for mining data on tumor development and patterns of metastases. MTB curators acquire data through manual curation of peer-reviewed scientific literature and from direct submissions by researchers. Data in MTB are also obtained from other bioinformatics resources including PathBase, the Gene Expression Omnibus and ArrayExpress. Recent enhancements to MTB improve the association between mouse models and human genes commonly mutated in a variety of cancers as identified in large-scale cancer genomics studies, provide new interfaces for exploring regions of the mouse genome associated with cancer phenotypes and incorporate data and information related to Patient-Derived Xenograft models of human cancers. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

Targeting NADPH oxidase decreases oxidative stress in the transgenic sickle cell mouse penis.

PubMed

Musicki, Biljana; Liu, Tongyun; Sezen, Sena F; Burnett, Arthur L

2012-08-01

Sickle cell disease (SCD) is a state of chronic vasculopathy characterized by endothelial dysfunction and increased oxidative stress, but the sources and mechanisms responsible for reactive oxygen species (ROS) production in the penis are unknown. We evaluated whether SCD activates NADPH oxidase, induces endothelial nitric oxide synthase (eNOS) uncoupling, and decreases antioxidants in the SCD mouse penis. We further tested the hypothesis that targeting NADPH oxidase decreases oxidative stress in the SCD mouse penis. SCD transgenic (sickle) mice were used as an animal model of SCD. Hemizygous (hemi) mice served as controls. Mice received an NADPH oxidase inhibitor apocynin (10 mM in drinking water) or vehicle. Penes were excised at baseline for molecular studies. Markers of oxidative stress (4-hydroxy-2-nonenal [HNE]), sources of ROS (eNOS uncoupling and NADPH oxidase subunits p67(phox) , p47(phox) , and gp91(phox) ), and enzymatic antioxidants (superoxide dismutase [SOD]1, SOD2, catalase, and glutathione peroxidase-1 [GPx1]) were measured by Western blot in penes. Sources of ROS, oxidative stress, and enzymatic antioxidants in the SCD penis. Relative to hemi mice, SCD increased (P<0.05) protein expression of NADPH oxidase subunits p67(phox) , p47(phox) , and gp91(phox) , 4-HNE-modified proteins, induced eNOS uncoupling, and reduced Gpx1 expression in the penis. Apocynin treatment of sickle mice reversed (P<0.05) the abnormalities in protein expressions of p47(phox) , gp91(phox) (but not p67(phox) ) and 4-HNE, but only slightly (P>0.05) prevented eNOS uncoupling in the penis. Apocynin treatment of hemi mice did not affect any of these parameters. NADPH oxidase and eNOS uncoupling are sources of oxidative stress in the SCD penis; decreased GPx1 further contributes to oxidative stress. Inhibition of NADPH oxidase upregulation decreases oxidative stress, implying a major role for NADPH oxidase as a ROS source and a potential target for improving vascular function in

Targeting NADPH Oxidase Decreases Oxidative Stress in the Transgenic Sickle Cell Mouse Penis

PubMed Central

Musicki, Biljana; Liu, Tongyun; Sezen, Sena F.; Burnett, Arthur L.

2012-01-01

Introduction Sickle cell disease (SCD) is a state of chronic vasculopathy characterized by endothelial dysfunction and increased oxidative stress, but the sources and mechanisms responsible for reactive oxygen species (ROS) production in the penis are unknown. Aims We evaluated whether SCD activates NADPH oxidase, induces endothelial nitric oxide synthase (eNOS) uncoupling, and decreases antioxidants in the SCD mouse penis. We further tested the hypothesis that targeting NADPH oxidase decreases oxidative stress in the SCD mouse penis. Methods SCD transgenic (sickle) mice were used as an animal model of SCD. Hemizygous (hemi) mice served as controls. Mice received an NADPH oxidase inhibitor apocynin (10 mM in drinking water) or vehicle. Penes were excised at baseline for molecular studies. Markers of oxidative stress (4-hydroxy-2-nonenal [HNE]), sources of ROS (eNOS uncoupling and NADPH oxidase subunits p67phox, p47phox, and gp91phox), and enzymatic antioxidants (superoxide dismutase [SOD]1, SOD2, catalase, and glutathione peroxidase-1 [GPx1]) were measured by Western blot in penes. Main Outcome Measures Sources of ROS, oxidative stress, and enzymatic antioxidants in the SCD penis. Results Relative to hemi mice, SCD increased (P < 0.05) protein expression of NADPH oxidase subunits p67phox, p47phox, and gp91phox, 4-HNE-modified proteins, induced eNOS uncoupling, and reduced Gpx1 expression in the penis. Apocynin treatment of sickle mice reversed (P < 0.05) the abnormalities in protein expressions of p47phox, gp91phox (but not p67phox) and 4-HNE, but only slightly (P > 0.05) prevented eNOS uncoupling in the penis. Apocynin treatment of hemi mice did not affect any of these parameters. Conclusion NADPH oxidase and eNOS uncoupling are sources of oxidative stress in the SCD penis; decreased GPx1 further contributes to oxidative stress. Inhibition of NADPH oxidase upregulation decreases oxidative stress, implying a major role for NADPH oxidase as a ROS source and a

40 CFR 91.801 - Applicability.

Code of Federal Regulations, 2011 CFR

2011-07-01

... under section 213 of the Act does apply. (e) Add the following paragraph to 40 CFR 85.1805 (a)(9): A....805 are waived for existing technology OB/PWC as defined in § 91.3 through model year 2003. (1) The... in sections 91.803 through 91.805 for existing technology OB/PWC for a specific engine family up to...

27 CFR 6.91 - Samples.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Samples. 6.91 Section 6.91 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS âTIED-HOUSEâ Exceptions § 6.91 Samples. The act by an industry member of furnishing or giving a sample of distilled spirits, wine, o...

Comparative toxicogenomic analysis of oral Cr(VI) exposure effects in rat and mouse small intestinal epithelia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kopec, Anna K.; Thompson, Chad M.; Kim, Suntae

2012-07-15

Continuous exposure to high concentrations of hexavalent chromium [Cr(VI)] in drinking water results in intestinal tumors in mice but not rats. Concentration-dependent gene expression effects were evaluated in female F344 rat duodenal and jejunal epithelia following 7 and 90 days of exposure to 0.3–520 mg/L (as sodium dichromate dihydrate, SDD) in drinking water. Whole-genome microarrays identified 3269 and 1815 duodenal, and 4557 and 1534 jejunal differentially expressed genes at 8 and 91 days, respectively, with significant overlaps between the intestinal segments. Functional annotation identified gene expression changes associated with oxidative stress, cell cycle, cell death, and immune response that weremore » consistent with reported changes in redox status and histopathology. Comparative analysis with B6C3F1 mouse data from a similarly designed study identified 2790 differentially expressed rat orthologs in the duodenum compared to 5013 mouse orthologs at day 8, and only 1504 rat and 3484 mouse orthologs at day 91. Automated dose–response modeling resulted in similar median EC{sub 50}s in the rodent duodenal and jejunal mucosae. Comparative examination of differentially expressed genes also identified divergently regulated orthologs. Comparable numbers of differentially expressed genes were observed at equivalent Cr concentrations (μg Cr/g duodenum). However, mice accumulated higher Cr levels than rats at ≥ 170 mg/L SDD, resulting in a ∼ 2-fold increase in the number of differentially expressed genes. These qualitative and quantitative differences in differential gene expression, which correlate with differences in tissue dose, likely contribute to the disparate intestinal tumor outcomes. -- Highlights: ► Cr(VI) elicits dose-dependent changes in gene expression in rat intestine. ► Cr(VI) elicits less differential gene expression in rats compared to mice. ► Cr(VI) gene expression can be phenotypically anchored to intestinal changes. �

40 CFR 91.1106 - Penalties.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... (1) A person who violates § 91.1103 (a)(1), (a)(4), or (a)(5), or a manufacturer or dealer who violates § 91.1103(a)(3)(i), is subject to a civil penalty of not more than $32,500 for each violation. (2) A person other than a manufacturer or dealer who violates § 91.1103(a)(3)(i) or any person who...

LBH589, A Hydroxamic Acid-Derived HDAC Inhibitor, is Neuroprotective in Mouse Models of Huntington’s Disease

PubMed Central

Chopra, Vanita; Quinti, Luisa; Khanna, Prarthana; Paganetti, Paolo; Kuhn, Rainer; Young, Anne B.; Kazantsev, Aleksey G.; Hersch, Steven

2016-01-01

Background: Modulation of gene transcription by HDAC inhibitors has been shown repeatedly to be neuroprotective in cellular, invertebrate, and rodent models of Huntington’s disease (HD). It has been difficult to translate these treatments to the clinic, however, because existing compounds have limited potency or brain bioavailability. Objective: In the present study, we assessed the therapeutic potential of LBH589, an orally bioavailable hydroxamic acid-derived nonselective HDAC inhibitor in mouse models of HD. Method: The efficacy of LBH589 is tested in two HD mouse models using various biochemical, behavioral and neuropathological outcome measures. Results: We show that LBH589 crosses the blood brain barrier; induces histone hyperacetylation and prevents striatal neuronal shrinkage in R6/2 HD mice. In full-length knock-in HD mice LBH589-treatment improves motor performance and reduces neuronal atrophy. Conclusions: Our efficacious results of LBH589 in fragment and full-length mouse models of HD suggest that LBH589 is a promising candidate for clinical assessment in HD patients and provides confirmation that non-selective HDAC inhibitors can be viable clinical candidates. PMID:27983565

Galaxy CloudMan: delivering cloud compute clusters

PubMed Central

2010-01-01

Background Widespread adoption of high-throughput sequencing has greatly increased the scale and sophistication of computational infrastructure needed to perform genomic research. An alternative to building and maintaining local infrastructure is “cloud computing”, which, in principle, offers on demand access to flexible computational infrastructure. However, cloud computing resources are not yet suitable for immediate “as is” use by experimental biologists. Results We present a cloud resource management system that makes it possible for individual researchers to compose and control an arbitrarily sized compute cluster on Amazon’s EC2 cloud infrastructure without any informatics requirements. Within this system, an entire suite of biological tools packaged by the NERC Bio-Linux team (http://nebc.nerc.ac.uk/tools/bio-linux) is available for immediate consumption. The provided solution makes it possible, using only a web browser, to create a completely configured compute cluster ready to perform analysis in less than five minutes. Moreover, we provide an automated method for building custom deployments of cloud resources. This approach promotes reproducibility of results and, if desired, allows individuals and labs to add or customize an otherwise available cloud system to better meet their needs. Conclusions The expected knowledge and associated effort with deploying a compute cluster in the Amazon EC2 cloud is not trivial. The solution presented in this paper eliminates these barriers, making it possible for researchers to deploy exactly the amount of computing power they need, combined with a wealth of existing analysis software, to handle the ongoing data deluge. PMID:21210983

Phenobarbital induces cell cycle transcriptional responses in mouse liver humanized for constitutive androstane and pregnane x receptors.

PubMed

Luisier, Raphaëlle; Lempiäinen, Harri; Scherbichler, Nina; Braeuning, Albert; Geissler, Miriam; Dubost, Valerie; Müller, Arne; Scheer, Nico; Chibout, Salah-Dine; Hara, Hisanori; Picard, Frank; Theil, Diethilde; Couttet, Philippe; Vitobello, Antonio; Grenet, Olivier; Grasl-Kraupp, Bettina; Ellinger-Ziegelbauer, Heidrun; Thomson, John P; Meehan, Richard R; Elcombe, Clifford R; Henderson, Colin J; Wolf, C Roland; Schwarz, Michael; Moulin, Pierre; Terranova, Rémi; Moggs, Jonathan G

2014-06-01

The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CAR(KO)-PXR(KO)), double humanized CAR and PXR (CAR(h)-PXR(h)), and wild-type C57BL/6 mice. Wild-type and CAR(h)-PXR(h) mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CAR(KO)-PXR(KO) mouse livers and largely reversible in wild-type and CAR(h)-PXR(h) mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CAR(h)-PXR(h) mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB.

Ultraviolet radiation directly induces pigment production by cultured human melanocytes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Friedmann, P.S.; Gilchrest, B.A.

1987-10-01

In humans the major stimulus for cutaneous pigmentation is ultraviolet radiation (UVR). Little is known about the mechanism underlying this response, in part because of the complexity of interactions in whole epidermis. Using a recently developed culture system, human melanocytes were exposed daily to a physiologic range of UVR doses from a solar simulator. Responses were determined 24 hours after the last exposure. There was a dose-related increase in melanin content per cell and uptake of /sup 14/C-DOPA, accompanied by growth inhibition. Cells from donors of different racial origin gave proportionately similar increases in melanin, although there were approximately tenfoldmore » differences in basal values. Light and electron microscopy revealed UVR-stimulated increases in dendricity as well as melanosome number and degree of melanization, analogous to the well-recognized melanocyte changes following sun exposure of intact skin. Similar responses were seen with Cloudman S91 melanoma cells, although this murine cell line required lower UVR dosages and fewer exposures for maximal stimulation. These data establish that UVR is capable of directly stimulating melanogenesis. Because cyclic AMP elevation has been associated in some settings with increased pigment production by cultured melanocytes, preliminary experiments were conducted to see if the effects of UVR were mediated by cAMP. Both alpha-MSH and isobutylmethylxanthine (IBMX), as positive controls, caused a fourfold increase in cAMP level in human melanocytes and/or S91 cells, but following a dose of UVR sufficient to stimulate pigment production there was no change in cAMP level up to 4 hours after exposure. Thus, it appears that the UVR-induced melanogenesis is mediated by cAMP-independent mechanisms.« less

14 CFR 91.219 - Altitude alerting system or device: Turbojet-powered civil airplanes.

Code of Federal Regulations, 2010 CFR

2010-01-01

...: Turbojet-powered civil airplanes. 91.219 Section 91.219 Aeronautics and Space FEDERAL AVIATION... system or device: Turbojet-powered civil airplanes. (a) Except as provided in paragraph (d) of this section, no person may operate a turbojet-powered U.S.-registered civil airplane unless that airplane is...

14 CFR 91.219 - Altitude alerting system or device: Turbojet-powered civil airplanes.

Code of Federal Regulations, 2013 CFR

2013-01-01

...: Turbojet-powered civil airplanes. 91.219 Section 91.219 Aeronautics and Space FEDERAL AVIATION... system or device: Turbojet-powered civil airplanes. (a) Except as provided in paragraph (d) of this section, no person may operate a turbojet-powered U.S.-registered civil airplane unless that airplane is...

14 CFR 91.219 - Altitude alerting system or device: Turbojet-powered civil airplanes.

Code of Federal Regulations, 2011 CFR

2011-01-01

...: Turbojet-powered civil airplanes. 91.219 Section 91.219 Aeronautics and Space FEDERAL AVIATION... system or device: Turbojet-powered civil airplanes. (a) Except as provided in paragraph (d) of this section, no person may operate a turbojet-powered U.S.-registered civil airplane unless that airplane is...

14 CFR 91.219 - Altitude alerting system or device: Turbojet-powered civil airplanes.

Code of Federal Regulations, 2012 CFR

2012-01-01

...: Turbojet-powered civil airplanes. 91.219 Section 91.219 Aeronautics and Space FEDERAL AVIATION... system or device: Turbojet-powered civil airplanes. (a) Except as provided in paragraph (d) of this section, no person may operate a turbojet-powered U.S.-registered civil airplane unless that airplane is...

14 CFR 91.219 - Altitude alerting system or device: Turbojet-powered civil airplanes.

Code of Federal Regulations, 2014 CFR

2014-01-01

...: Turbojet-powered civil airplanes. 91.219 Section 91.219 Aeronautics and Space FEDERAL AVIATION... system or device: Turbojet-powered civil airplanes. (a) Except as provided in paragraph (d) of this section, no person may operate a turbojet-powered U.S.-registered civil airplane unless that airplane is...

40 CFR 427.91 - Specialized definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Specialized definitions. 427.91 Section 427.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ASBESTOS MANUFACTURING POINT SOURCE CATEGORY Solvent Recovery Subcategory § 427.91...

14 CFR 91.1111 - Maintenance training.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Maintenance training. 91.1111 Section 91... Operations Program Management § 91.1111 Maintenance training. The program manager must ensure that all... annual recurrent training and are competent to perform those duties. ...

14 CFR 91.1111 - Maintenance training.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Maintenance training. 91.1111 Section 91... Operations Program Management § 91.1111 Maintenance training. The program manager must ensure that all... annual recurrent training and are competent to perform those duties. ...

40 CFR 141.91 - Recordkeeping requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Recordkeeping requirements. 141.91 Section 141.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Control of Lead and Copper § 141.91 Recordkeeping...

40 CFR 141.91 - Recordkeeping requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Recordkeeping requirements. 141.91 Section 141.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Control of Lead and Copper § 141.91 Recordkeeping...

7 CFR 91.21 - Protecting samples.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 3 2011-01-01 2011-01-01 false Protecting samples. 91.21 Section 91.21 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... SERVICES AND GENERAL INFORMATION Samples § 91.21 Protecting samples. Laboratory personnel shall protect...

7 CFR 91.21 - Protecting samples.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 3 2013-01-01 2013-01-01 false Protecting samples. 91.21 Section 91.21 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... SERVICES AND GENERAL INFORMATION Samples § 91.21 Protecting samples. Laboratory personnel shall protect...

7 CFR 91.21 - Protecting samples.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false Protecting samples. 91.21 Section 91.21 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... SERVICES AND GENERAL INFORMATION Samples § 91.21 Protecting samples. Laboratory personnel shall protect...

7 CFR 91.21 - Protecting samples.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 3 2012-01-01 2012-01-01 false Protecting samples. 91.21 Section 91.21 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... SERVICES AND GENERAL INFORMATION Samples § 91.21 Protecting samples. Laboratory personnel shall protect...

7 CFR 91.21 - Protecting samples.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 3 2014-01-01 2014-01-01 false Protecting samples. 91.21 Section 91.21 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... SERVICES AND GENERAL INFORMATION Samples § 91.21 Protecting samples. Laboratory personnel shall protect...

Sequence and Characterization of the Ig Heavy Chain Constant and Partial Variable Region of the Mouse Strain 129S11

PubMed Central

Retter, Ida; Chevillard, Christophe; Scharfe, Maren; Conrad, Ansgar; Hafner, Martin; Im, Tschong-Hun; Ludewig, Monika; Nordsiek, Gabriele; Severitt, Simone; Thies, Stephanie; Mauhar, America; Blöcker, Helmut; Müller, Werner; Riblet, Roy

2009-01-01

Although the entire mouse genome has been sequenced, there remain challenges concerning the elucidation of particular complex and polymorphic genomic loci. In the murine Igh locus, different haplotypes exist in different inbred mouse strains. For example, the Ighb haplotype sequence of the Mouse Genome Project strain C57BL/6 differs considerably from the Igha haplotype of BALB/c, which has been widely used in the analyses of Ab responses. We have sequenced and annotated the 3′ half of the Igha locus of 129S1/SvImJ, covering the CH region and approximately half of the VH region. This sequence comprises 128 VH genes, of which 49 are judged to be functional. The comparison of the Igha sequence with the homologous Ighb region from C57BL/6 revealed two major expansions in the germline repertoire of Igha. In addition, we found smaller haplotype-specific differences like the duplication of five VH genes in the Igha locus. We generated a VH allele table by comparing the individual VH genes of both haplotypes. Surprisingly, the number and position of DH genes in the 129S1 strain differs not only from the sequence of C57BL/6 but also from the map published for BALB/c. Taken together, the contiguous genomic sequence of the 3′ part of the Igha locus allows a detailed view of the recent evolution of this highly dynamic locus in the mouse. PMID:17675503

40 CFR 406.91 - Specialized definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 406.91 Section 406.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS GRAIN MILLS POINT SOURCE CATEGORY Ready-to-Eat Cereal Subcategory § 406.91 Specialized definitions...

A novel approach for reliable detection of cathepsin S activities in mouse antigen presenting cells.

PubMed

Steimle, Alex; Kalbacher, Hubert; Maurer, Andreas; Beifuss, Brigitte; Bender, Annika; Schäfer, Andrea; Müller, Ricarda; Autenrieth, Ingo B; Frick, Julia-Stefanie

2016-05-01

Cathepsin S (CTSS) is a eukaryotic protease mostly expressed in professional antigen presenting cells (APCs). Since CTSS activity regulation plays a role in the pathogenesis of various autoimmune diseases like multiple sclerosis, atherosclerosis, Sjögren's syndrome and psoriasis as well as in cancer progression, there is an ongoing interest in the reliable detection of cathepsin S activity. Various applications have been invented for specific detection of this enzyme. However, most of them have only been shown to be suitable for human samples, do not deliver quantitative results or the experimental procedure requires technical equipment that is not commonly available in a standard laboratory. We have tested a fluorogen substrate, Mca-GRWPPMGLPWE-Lys(Dnp)-DArg-NH2, that has been described to specifically detect CTSS activities in human APCs for its potential use for mouse samples. We have modified the protocol and thereby offer a cheap, easy, reproducible and quick activity assay to detect CTSS activities in mouse APCs. Since most of basic research on CTSS is performed in mice, this method closes a gap and offers a possibility for reliable and quantitative CTSS activity detection that can be performed in almost every laboratory. Copyright © 2016. Published by Elsevier B.V.

7 CFR 91.23 - Analytical methods.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 3 2014-01-01 2014-01-01 false Analytical methods. 91.23 Section 91.23 Agriculture... SERVICES AND GENERAL INFORMATION Method Manuals § 91.23 Analytical methods. Most analyses are performed according to approved procedures described in manuals of standardized methodology. These standard methods...

7 CFR 91.23 - Analytical methods.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 3 2011-01-01 2011-01-01 false Analytical methods. 91.23 Section 91.23 Agriculture... SERVICES AND GENERAL INFORMATION Method Manuals § 91.23 Analytical methods. Most analyses are performed according to approved procedures described in manuals of standardized methodology. These standard methods...

7 CFR 91.23 - Analytical methods.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 3 2013-01-01 2013-01-01 false Analytical methods. 91.23 Section 91.23 Agriculture... SERVICES AND GENERAL INFORMATION Method Manuals § 91.23 Analytical methods. Most analyses are performed according to approved procedures described in manuals of standardized methodology. These standard methods...

28 CFR 2.91 - Supervision responsibility.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Supervision responsibility. 2.91 Section 2.91 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF....91 Supervision responsibility. (a) Pursuant to D.C. Code 24-133(c), the District of Columbia Court...

28 CFR 2.91 - Supervision responsibility.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Supervision responsibility. 2.91 Section 2.91 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF....91 Supervision responsibility. (a) Pursuant to D.C. Code 24-133(c), the District of Columbia Court...

28 CFR 2.91 - Supervision responsibility.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 1 2012-07-01 2012-07-01 false Supervision responsibility. 2.91 Section 2.91 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF....91 Supervision responsibility. (a) Pursuant to D.C. Code 24-133(c), the District of Columbia Court...

28 CFR 2.91 - Supervision responsibility.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 1 2013-07-01 2013-07-01 false Supervision responsibility. 2.91 Section 2.91 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF....91 Supervision responsibility. (a) Pursuant to D.C. Code 24-133(c), the District of Columbia Court...

28 CFR 2.91 - Supervision responsibility.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 1 2014-07-01 2014-07-01 false Supervision responsibility. 2.91 Section 2.91 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF....91 Supervision responsibility. (a) Pursuant to D.C. Code 24-133(c), the District of Columbia Court...

50 CFR 91.4 - Eligible species.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Eligible species. 91.4 Section 91.4 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MISCELLANEOUS PROVISIONS MIGRATORY BIRD HUNTING AND CONSERVATION STAMP CONTEST Introduction § 91.4 Eligible...

50 CFR 91.4 - Eligible species.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false Eligible species. 91.4 Section 91.4 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MISCELLANEOUS PROVISIONS MIGRATORY BIRD HUNTING AND CONSERVATION STAMP CONTEST Introduction § 91.4 Eligible...

40 CFR 91.604 - Test orders.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Test orders. 91.604 Section 91.604 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Selective Enforcement Auditing Regulations § 91.604 Test orders...

1 CFR 9.1 - Publication required.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 1 General Provisions 1 2011-01-01 2011-01-01 false Publication required. 9.1 Section 9.1 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER THE UNITED STATES GOVERNMENT MANUAL § 9.1 Publication required. The Director of the Federal Register shall...

10 CFR 76.91 - Emergency planning.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 2 2013-01-01 2013-01-01 false Emergency planning. 76.91 Section 76.91 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) CERTIFICATION OF GASEOUS DIFFUSION PLANTS Safety § 76.91 Emergency planning. The Corporation shall establish, maintain, and be prepared to follow a written emergency plan. The...

10 CFR 76.91 - Emergency planning.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 2 2014-01-01 2014-01-01 false Emergency planning. 76.91 Section 76.91 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) CERTIFICATION OF GASEOUS DIFFUSION PLANTS Safety § 76.91 Emergency planning. The Corporation shall establish, maintain, and be prepared to follow a written emergency plan. The...

10 CFR 76.91 - Emergency planning.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Emergency planning. 76.91 Section 76.91 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) CERTIFICATION OF GASEOUS DIFFUSION PLANTS Safety § 76.91 Emergency planning. The Corporation shall establish, maintain, and be prepared to follow a written emergency plan. The...

14 CFR 91.517 - Passenger information.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Passenger information. 91.517 Section 91... Multiengine Airplanes and Fractional Ownership Program Aircraft § 91.517 Passenger information. (a) Except as... belts and when smoking is prohibited. (c) If passenger information signs are installed, no passenger or...

40 CFR 91.311 - Test conditions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Test conditions. 91.311 Section 91.311... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Emission Test Equipment Provisions § 91.311 Test conditions. (a) General requirements. (1) Ambient temperature levels encountered by the test engine throughout the test...

32 CFR 842.91 - Settlement agreements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 6 2014-07-01 2014-07-01 false Settlement agreements. 842.91 Section 842.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION...) § 842.91 Settlement agreements. The claimant must sign a settlement agreement and general release before...

32 CFR 842.91 - Settlement agreements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Settlement agreements. 842.91 Section 842.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION...) § 842.91 Settlement agreements. The claimant must sign a settlement agreement and general release before...

32 CFR 842.91 - Settlement agreements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false Settlement agreements. 842.91 Section 842.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE CLAIMS AND LITIGATION...) § 842.91 Settlement agreements. The claimant must sign a settlement agreement and general release before...

40 CFR 91.2 - Applicable date.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Applicable date. 91.2 Section 91.2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES General § 91.2 Applicable date. This part applies to marine...

1 CFR 9.1 - Publication required.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 1 General Provisions 1 2012-01-01 2012-01-01 false Publication required. 9.1 Section 9.1 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER THE UNITED STATES GOVERNMENT MANUAL § 9.1 Publication required. (a) The Director publishes a special edition...

1 CFR 9.1 - Publication required.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 1 General Provisions 1 2014-01-01 2012-01-01 true Publication required. 9.1 Section 9.1 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER THE UNITED STATES GOVERNMENT MANUAL § 9.1 Publication required. (a) The Director publishes a special edition...

1 CFR 9.1 - Publication required.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 1 General Provisions 1 2013-01-01 2012-01-01 true Publication required. 9.1 Section 9.1 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER SPECIAL EDITIONS OF THE FEDERAL REGISTER THE UNITED STATES GOVERNMENT MANUAL § 9.1 Publication required. (a) The Director publishes a special edition...

24 CFR 91.315 - Strategic plan.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Strategic plan. 91.315 Section 91... CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS State Governments; Contents of Consolidated Plan § 91.315 Strategic plan. (a) General. For the categories described in paragraphs (b), (c), (d...

Sex and gonadal hormones in mouse models of Alzheimer’s disease: what is relevant to the human condition?

PubMed Central

2012-01-01

Biologic sex and gonadal hormones matter in human aging and diseases of aging such as Alzheimer’s – and the importance of studying their influences relates directly to human health. The goal of this article is to review the literature to date on sex and hormones in mouse models of Alzheimer’s disease (AD) with an exclusive focus on interpreting the relevance of findings to the human condition. To this end, we highlight advances in AD and in sex and hormone biology, discuss what these advances mean for merging the two fields, review the current mouse model literature, raise major unresolved questions, and offer a research framework that incorporates human reproductive aging for future studies aimed at translational discoveries in this important area. Unraveling human relevant pathways in sex and hormone-based biology may ultimately pave the way to novel and urgently needed treatments for AD and other neurodegenerative diseases. PMID:23126652

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. A motor vehicle does not conform to the visual exhaust system inspection requirements, 40 CFR 202.22, of the Interstate Motor Carrier...

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 5 2012-10-01 2012-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. A motor vehicle does not conform to the visual exhaust system inspection requirements, 40 CFR 202.22, of the Interstate Motor Carrier...

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. A motor vehicle does not conform to the visual exhaust system inspection requirements, 40 CFR 202.22, of the Interstate Motor Carrier...

40 CFR 91.604 - Test orders.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Test orders. 91.604 Section 91.604... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Selective Enforcement Auditing Regulations § 91.604 Test orders. (a) A test order addressed to the manufacturer is required for any testing under this subpart. (b...

40 CFR 91.607 - Test procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Test procedures. 91.607 Section 91.607... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Selective Enforcement Auditing Regulations § 91.607 Test procedures. (a)(1) For marine SI engines subject to the provisions of this subpart, the prescribed test...

40 CFR 91.507 - Test procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Test procedures. 91.507 Section 91.507... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Manufacturer Production Line Testing Program § 91.507 Test procedures. (a)(1) For marine SI engines subject to the provisions of this subpart, the prescribed test...

40 CFR 91.507 - Test procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Test procedures. 91.507 Section 91.507... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Manufacturer Production Line Testing Program § 91.507 Test procedures. (a)(1) For marine SI engines subject to the provisions of this subpart, the prescribed test...

25 CFR 700.91 - Relocation report.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 2 2011-04-01 2011-04-01 false Relocation report. 700.91 Section 700.91 Indians THE... Policies and Instructions Definitions § 700.91 Relocation report. The relocation report shall be the report prepared by the Commission and submitted to Congress pursuant to section 13(a) of the Act. ...

25 CFR 700.91 - Relocation report.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 25 Indians 2 2012-04-01 2012-04-01 false Relocation report. 700.91 Section 700.91 Indians THE... Policies and Instructions Definitions § 700.91 Relocation report. The relocation report shall be the report prepared by the Commission and submitted to Congress pursuant to section 13(a) of the Act. ...

25 CFR 700.91 - Relocation report.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false Relocation report. 700.91 Section 700.91 Indians THE... Policies and Instructions Definitions § 700.91 Relocation report. The relocation report shall be the report prepared by the Commission and submitted to Congress pursuant to section 13(a) of the Act. ...

25 CFR 700.91 - Relocation report.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 25 Indians 2 2014-04-01 2014-04-01 false Relocation report. 700.91 Section 700.91 Indians THE... Policies and Instructions Definitions § 700.91 Relocation report. The relocation report shall be the report prepared by the Commission and submitted to Congress pursuant to section 13(a) of the Act. ...

25 CFR 700.91 - Relocation report.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 25 Indians 2 2013-04-01 2013-04-01 false Relocation report. 700.91 Section 700.91 Indians THE... Policies and Instructions Definitions § 700.91 Relocation report. The relocation report shall be the report prepared by the Commission and submitted to Congress pursuant to section 13(a) of the Act. ...

40 CFR 98.91 - Reporting threshold.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 22 2013-07-01 2013-07-01 false Reporting threshold. 98.91 Section 98.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Electronics Manufacturing § 98.91 Reporting threshold. (a) You must report GHG emissions under this subpart if...

40 CFR 98.91 - Reporting threshold.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 21 2011-07-01 2011-07-01 false Reporting threshold. 98.91 Section 98.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Electronics Manufacturing § 98.91 Reporting threshold. (a) You must report GHG emissions under this subpart if...

40 CFR 98.91 - Reporting threshold.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 21 2014-07-01 2014-07-01 false Reporting threshold. 98.91 Section 98.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Electronics Manufacturing § 98.91 Reporting threshold. (a) You must report GHG emissions under this subpart if...

14 CFR 91.303 - Aerobatic flight.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Aerobatic flight. 91.303 Section 91.303... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.303 Aerobatic flight. No person may operate an aircraft in aerobatic flight— (a) Over any congested area of a...

14 CFR 91.303 - Aerobatic flight.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Aerobatic flight. 91.303 Section 91.303... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.303 Aerobatic flight. No person may operate an aircraft in aerobatic flight— (a) Over any congested area of a...

14 CFR 91.303 - Aerobatic flight.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Aerobatic flight. 91.303 Section 91.303... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.303 Aerobatic flight. No person may operate an aircraft in aerobatic flight— (a) Over any congested area of a...

14 CFR 91.303 - Aerobatic flight.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Aerobatic flight. 91.303 Section 91.303... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.303 Aerobatic flight. No person may operate an aircraft in aerobatic flight— (a) Over any congested area of a...

14 CFR 91.303 - Aerobatic flight.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Aerobatic flight. 91.303 Section 91.303... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.303 Aerobatic flight. No person may operate an aircraft in aerobatic flight— (a) Over any congested area of a...

9 CFR 91.20 - General construction.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false General construction. 91.20 Section 91... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.20 General construction. A variety of construction materials such as wood, metal plate, or pipe may be used for stalls, crates, or pens...

45 CFR 91.34 - Information requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Information requirements. 91.34 Section 91.34... Recipients § 91.34 Information requirements. Each recipient shall: (a) Keep records in a form and containing information which HHS determines may be necessary to ascertain whether the recipient is complying with the Act...

9 CFR 91.26 - Concrete flooring.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Concrete flooring. 91.26 Section 91.26... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.26 Concrete flooring. (a) Pens aboard an ocean vessel shall have a 3 inch concrete pavement, proportioned and mixed to give 2000 psi...

45 CFR 98.91 - Non-compliance.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 1 2012-10-01 2012-10-01 false Non-compliance. 98.91 Section 98.91 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION CHILD CARE AND DEVELOPMENT FUND Monitoring, Non-compliance and Complaints § 98.91 Non-compliance. (a) If after reasonable notice to a Lead Agency...

49 CFR 325.91 - Exhaust systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 5 2010-10-01 2010-10-01 false Exhaust systems. 325.91 Section 325.91... EMISSION STANDARDS Exhaust Systems and Tires § 325.91 Exhaust systems. Link to an amendment published at 75 FR 57193, Sept. 20, 2010. A motor vehicle does not conform to the visual exhaust system inspection...

46 CFR 91.20-10 - Plans.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Plans. 91.20-10 Section 91.20-10 Shipping COAST GUARD... Initial Inspection § 91.20-10 Plans. (a) Before application for inspection is made, and before construction is started, the owner or builder shall have plans approved by the Commandant indicating the...

24 CFR 91.415 - Strategic plan.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Strategic plan. 91.415 Section 91... Plan § 91.415 Strategic plan. Strategies and priority needs must be described in the consolidated plan... addressing underserved needs. (Approved by the Office of Management and Budget under control number 2506-0117) ...

24 CFR 91.415 - Strategic plan.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Strategic plan. 91.415 Section 91... Plan § 91.415 Strategic plan. Strategies and priority needs must be described in the consolidated plan... addressing underserved needs. (Approved by the Office of Management and Budget under control number 2506-0117) ...

45 CFR 91.41 - Compliance reviews.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Compliance reviews. 91.41 Section 91.41 Public..., Conciliation, and Enforcement Procedures § 91.41 Compliance reviews. (a) HHS may conduct compliance reviews and... the Act and these regulations has occurred. (b) If a compliance review or pre-award review indicates a...

9 CFR 91.3 - General export requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

.... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

9 CFR 91.3 - General export requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

.... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

9 CFR 91.3 - General export requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

.... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

9 CFR 91.3 - General export requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

.... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

14 CFR 141.91 - Satellite bases.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Satellite bases. 141.91 Section 141.91... OTHER CERTIFICATED AGENCIES PILOT SCHOOLS Operating Rules § 141.91 Satellite bases. The holder of a... training in an approved course of training at a base other than its main operations base if: (a) An...

14 CFR 141.91 - Satellite bases.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Satellite bases. 141.91 Section 141.91... OTHER CERTIFICATED AGENCIES PILOT SCHOOLS Operating Rules § 141.91 Satellite bases. The holder of a... training in an approved course of training at a base other than its main operations base if: (a) An...

14 CFR 141.91 - Satellite bases.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Satellite bases. 141.91 Section 141.91... OTHER CERTIFICATED AGENCIES PILOT SCHOOLS Operating Rules § 141.91 Satellite bases. The holder of a... training in an approved course of training at a base other than its main operations base if: (a) An...

14 CFR 141.91 - Satellite bases.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Satellite bases. 141.91 Section 141.91... OTHER CERTIFICATED AGENCIES PILOT SCHOOLS Operating Rules § 141.91 Satellite bases. The holder of a... training in an approved course of training at a base other than its main operations base if: (a) An...

24 CFR 91.220 - Action plan.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Action plan. 91.220 Section 91.220... Consolidated Plan § 91.220 Action plan. The action plan must include the following: (a) Standard Form 424; (b... to be undertaken. The action plan must provide a description of the activities the jurisdiction will...

24 CFR 91.220 - Action plan.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Action plan. 91.220 Section 91.220... Consolidated Plan § 91.220 Action plan. The action plan must include the following: (a) Standard Form 424; (b... to be undertaken. The action plan must provide a description of the activities the jurisdiction will...

24 CFR 91.420 - Action plan.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Action plan. 91.420 Section 91.420... Plan § 91.420 Action plan. (a) Form application. The action plan for the consortium must include a...) Description of resources and activities. The action plan must describe the resources to be used and activities...

24 CFR 91.420 - Action plan.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Action plan. 91.420 Section 91.420... Plan § 91.420 Action plan. (a) Form application. The action plan for the consortium must include a...) Description of resources and activities. The action plan must describe the resources to be used and activities...

45 CFR 91.46 - Compliance procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Compliance procedure. 91.46 Section 91.46 Public..., Conciliation, and Enforcement Procedures § 91.46 Compliance procedure. (a) HHS may enforce the Act and these... compliance cannot be obtained. (2) Thirty days have elapsed after the Secretary has sent a written report of...

7 CFR 91.1 - General.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false General. 91.1 Section 91.1 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) COMMODITY LABORATORY TESTING PROGRAMS SERVICES...

12 CFR 145.91 - Home office.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 1 2012-01-01 2012-01-01 false Home office. 145.91 Section 145.91 Banks and... § 145.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OCC licensing office if the permanent...

12 CFR 545.91 - Home office.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Home office. 545.91 Section 545.91 Banks and... § 545.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OTS Regional Office if the permanent...

12 CFR 145.91 - Home office.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 1 2013-01-01 2013-01-01 false Home office. 145.91 Section 145.91 Banks and... § 145.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OCC licensing office if the permanent...

12 CFR 545.91 - Home office.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 6 2014-01-01 2012-01-01 true Home office. 545.91 Section 545.91 Banks and... § 545.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OTS Regional Office if the permanent...

12 CFR 545.91 - Home office.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 6 2012-01-01 2012-01-01 false Home office. 545.91 Section 545.91 Banks and... § 545.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OTS Regional Office if the permanent...

12 CFR 545.91 - Home office.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 5 2011-01-01 2011-01-01 false Home office. 545.91 Section 545.91 Banks and... § 545.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OTS Regional Office if the permanent...

12 CFR 145.91 - Home office.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 1 2014-01-01 2014-01-01 false Home office. 145.91 Section 145.91 Banks and... § 145.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OCC licensing office if the permanent...

12 CFR 545.91 - Home office.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 6 2013-01-01 2012-01-01 true Home office. 545.91 Section 545.91 Banks and... § 545.91 Home office. (a) All operations of a Federal savings association (“you”) are subject to direction from the home office. (b) You must notify the appropriate OTS Regional Office if the permanent...

24 CFR 91.320 - Action plan.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Action plan. 91.320 Section 91.320... Consolidated Plan § 91.320 Action plan. The action plan must include the following: (a) Standard Form 424; (b... outcome measures for activities included in its action plan in accordance with guidance issued by HUD. For...

24 CFR 91.320 - Action plan.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Action plan. 91.320 Section 91.320... Consolidated Plan § 91.320 Action plan. The action plan must include the following: (a) Standard Form 424; (b... outcome measures for activities included in its action plan in accordance with guidance issued by HUD. For...

14 CFR 91.209 - Aircraft lights.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Aircraft lights. 91.209 Section 91.209... Requirements § 91.209 Aircraft lights. No person may: (a) During the period from sunset to sunrise (or, in... position lights; (2) Park or move an aircraft in, or in dangerous proximity to, a night flight operations...

14 CFR 91.209 - Aircraft lights.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Aircraft lights. 91.209 Section 91.209... Requirements § 91.209 Aircraft lights. No person may: (a) During the period from sunset to sunrise (or, in... position lights; (2) Park or move an aircraft in, or in dangerous proximity to, a night flight operations...

14 CFR 91.209 - Aircraft lights.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Aircraft lights. 91.209 Section 91.209... Requirements § 91.209 Aircraft lights. No person may: (a) During the period from sunset to sunrise (or, in... position lights; (2) Park or move an aircraft in, or in dangerous proximity to, a night flight operations...

14 CFR 91.209 - Aircraft lights.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Aircraft lights. 91.209 Section 91.209... Requirements § 91.209 Aircraft lights. No person may: (a) During the period from sunset to sunrise (or, in... position lights; (2) Park or move an aircraft in, or in dangerous proximity to, a night flight operations...

14 CFR 91.209 - Aircraft lights.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Aircraft lights. 91.209 Section 91.209... Requirements § 91.209 Aircraft lights. No person may: (a) During the period from sunset to sunrise (or, in... position lights; (2) Park or move an aircraft in, or in dangerous proximity to, a night flight operations...

45 CFR 98.91 - Non-compliance.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Non-compliance. 98.91 Section 98.91 Public Welfare..., Non-compliance and Complaints § 98.91 Non-compliance. (a) If after reasonable notice to a Lead Agency... the Lead Agency a written notice of a finding of non-compliance. This notice will be issued within 60...

14 CFR 91.323 - Increased maximum certificated weights for certain airplanes operated in Alaska.

Code of Federal Regulations, 2010 CFR

2010-01-01

... certain airplanes operated in Alaska. 91.323 Section 91.323 Aeronautics and Space FEDERAL AVIATION... certain airplanes operated in Alaska. (a) Notwithstanding any other provision of the Federal Aviation... certificated weight of an airplane type certificated under Aeronautics Bulletin No. 7-A of the U.S. Department...

14 CFR 91.323 - Increased maximum certificated weights for certain airplanes operated in Alaska.

Code of Federal Regulations, 2013 CFR

2013-01-01

... certain airplanes operated in Alaska. 91.323 Section 91.323 Aeronautics and Space FEDERAL AVIATION... certain airplanes operated in Alaska. (a) Notwithstanding any other provision of the Federal Aviation... certificated weight of an airplane type certificated under Aeronautics Bulletin No. 7-A of the U.S. Department...

14 CFR 91.323 - Increased maximum certificated weights for certain airplanes operated in Alaska.

Code of Federal Regulations, 2011 CFR

2011-01-01

... certain airplanes operated in Alaska. 91.323 Section 91.323 Aeronautics and Space FEDERAL AVIATION... certain airplanes operated in Alaska. (a) Notwithstanding any other provision of the Federal Aviation... certificated weight of an airplane type certificated under Aeronautics Bulletin No. 7-A of the U.S. Department...

14 CFR 91.323 - Increased maximum certificated weights for certain airplanes operated in Alaska.

Code of Federal Regulations, 2012 CFR

2012-01-01

... certain airplanes operated in Alaska. 91.323 Section 91.323 Aeronautics and Space FEDERAL AVIATION... certain airplanes operated in Alaska. (a) Notwithstanding any other provision of the Federal Aviation... certificated weight of an airplane type certificated under Aeronautics Bulletin No. 7-A of the U.S. Department...

14 CFR 91.323 - Increased maximum certificated weights for certain airplanes operated in Alaska.

Code of Federal Regulations, 2014 CFR

2014-01-01

... certain airplanes operated in Alaska. 91.323 Section 91.323 Aeronautics and Space FEDERAL AVIATION... certain airplanes operated in Alaska. (a) Notwithstanding any other provision of the Federal Aviation... certificated weight of an airplane type certificated under Aeronautics Bulletin No. 7-A of the U.S. Department...

Job Language Performance Requirements for MOS 91E, Dental Specialist, Reference Soldier’s Manual Dated 30 August 1977.

DTIC Science & Technology

1977-08-30

lubricate the air turbine driven straight, dental handpiece (Tru-Torc) L 081-91E-3768 Clean and lubricate the contra-angle, latch type dental handpiece 081...91E-3769 Clean and lubricate the Midwest "Quiet-Air"Ultra speed, contra-angle dental handpiece 081-91E-3810 Sharpen scaling instruments by using rotary...b-Al21 845 JOB LANGUAGE PERFORMANCE REQUIREMENTS FOR NOS 9iE In2 DENTAL SPECIALIST REFER.. (U) DEFENSE LANGUAGE INST LACKLAND AFB TX ENGLISH LANGUAGE

28 CFR 91.52 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Definitions. 91.52 Section 91.52 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) GRANTS FOR CORRECTIONAL FACILITIES Environmental Impact Review... on Environmental Quality in its Regulations for Implementing the Procedural Provisions of the...

28 CFR 91.52 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Definitions. 91.52 Section 91.52 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) GRANTS FOR CORRECTIONAL FACILITIES Environmental Impact Review... on Environmental Quality in its Regulations for Implementing the Procedural Provisions of the...

28 CFR 91.52 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Definitions. 91.52 Section 91.52 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) GRANTS FOR CORRECTIONAL FACILITIES Environmental Impact Review... on Environmental Quality in its Regulations for Implementing the Procedural Provisions of the...

28 CFR 91.52 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Definitions. 91.52 Section 91.52 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) GRANTS FOR CORRECTIONAL FACILITIES Environmental Impact Review... on Environmental Quality in its Regulations for Implementing the Procedural Provisions of the...

28 CFR 91.52 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Definitions. 91.52 Section 91.52 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) GRANTS FOR CORRECTIONAL FACILITIES Environmental Impact Review... on Environmental Quality in its Regulations for Implementing the Procedural Provisions of the...

7 CFR 966.91 - Separability.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Separability. 966.91 Section 966.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TOMATOES GROWN IN FLORIDA Order...

Sildenafil Promotes eNOS Activation and Inhibits NADPH Oxidase in the Transgenic Sickle Cell Mouse Penis

PubMed Central

Musicki, Biljana; Bivalacqua, Trinity J.; Champion, Hunter C.; Burnett, Arthur L.

2014-01-01

Introduction Sickle cell disease (SCD)-associated vasculopathy in the penis is characterized by aberrant nitric oxide and phosphodiesterase (PDE) 5 signaling, and by increased oxidative stress. Preliminary clinical trials show that continuous treatment with PDE5 inhibitor sildenafil unassociated with sexual activity decreases priapic activity in patients with SCD. However, the mechanism of its vasculoprotective effect in the penis remains unclear. Aims We evaluated whether continuous administration of PDE5 inhibitor sildenafil promotes eNOS function at posttranslational levels and decreases superoxide-producing enzyme NADPH oxidase activity in the sickle cell mouse penis. Methods SCD transgenic mice were used as an animal model of SCD. WT mice served as controls. Mice received treatment with the PDE5 inhibitor sildenafil (100 mg/kg/day) or vehicle for 3 weeks. eNOS phosphorylation on Ser-1177 (positive regulatory site), eNOS interactions with heat-shock protein 90 (HSP90) (positive regulator), phosphorylated AKT (upstream mediator of eNOS phosphorylation on Ser-1177), an NADPH oxidase catalytic subunit gp91(phox), and a marker of oxidative stress (4-hydroxy-2-nonenal [HNE]) were measured by Western blot. Main Outcome Measures Effect of continuous sildenafil treatment on eNOS posttranslational activation, NADPH oxidase catalytic subunit, and oxidative stress in the penis of the sickle cell mouse. Results Continuous treatment with sildenafil reversed (P < 0.05) the abnormalities in protein expressions of P-eNOS (Ser-1177), eNOS/HSP90 interaction, P-AKT, protein expression of gp91(phox), and 4-HNE, in the sickle cell mouse penis. Sildenafil treatment of WT mice did not affect any of these parameters. Conclusion Our findings that sildenafil enhances eNOS activation and inhibits NADPH oxidase function in the sickle cell mouse penis offers a vasculoprotective molecular basis for the therapeutic effect of sildenafil in the penis in association with SCD. PMID:24251665

Sildenafil promotes eNOS activation and inhibits NADPH oxidase in the transgenic sickle cell mouse penis.

PubMed

Musicki, Biljana; Bivalacqua, Trinity J; Champion, Hunter C; Burnett, Arthur L

2014-02-01

Sickle cell disease (SCD)-associated vasculopathy in the penis is characterized by aberrant nitric oxide and phosphodiesterase (PDE) 5 signaling, and by increased oxidative stress. Preliminary clinical trials show that continuous treatment with PDE5 inhibitor sildenafil unassociated with sexual activity decreases priapic activity in patients with SCD. However, the mechanism of its vasculoprotective effect in the penis remains unclear. We evaluated whether continuous administration of PDE5 inhibitor sildenafil promotes eNOS function at posttranslational levels and decreases superoxide-producing enzyme NADPH oxidase activity in the sickle cell mouse penis. SCD transgenic mice were used as an animal model of SCD. WT mice served as controls. Mice received treatment with the PDE5 inhibitor sildenafil (100 mg/kg/day) or vehicle for 3 weeks. eNOS phosphorylation on Ser-1177 (positive regulatory site), eNOS interactions with heat-shock protein 90 (HSP90) (positive regulator), phosphorylated AKT (upstream mediator of eNOS phosphorylation on Ser-1177), an NADPH oxidase catalytic subunit gp91(phox), and a marker of oxidative stress (4-hydroxy-2-nonenal [HNE]) were measured by Western blot. Effect of continuous sildenafil treatment on eNOS posttranslational activation, NADPH oxidase catalytic subunit, and oxidative stress in the penis of the sickle cell mouse. Continuous treatment with sildenafil reversed (P < 0.05) the abnormalities in protein expressions of P-eNOS (Ser-1177), eNOS/HSP90 interaction, P-AKT, protein expression of gp91(phox), and 4-HNE, in the sickle cell mouse penis. Sildenafil treatment of WT mice did not affect any of these parameters. Our findings that sildenafil enhances eNOS activation and inhibits NADPH oxidase function in the sickle cell mouse penis offers a vasculoprotective molecular basis for the therapeutic effect of sildenafil in the penis in association with SCD. © 2013 International Society for Sexual Medicine.

24 CFR 91.230 - Monitoring.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Monitoring. 91.230 Section 91.230 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

24 CFR 91.230 - Monitoring.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Monitoring. 91.230 Section 91.230 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

24 CFR 91.230 - Monitoring.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Monitoring. 91.230 Section 91.230 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

24 CFR 91.200 - General.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false General. 91.200 Section 91.200 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

24 CFR 91.230 - Monitoring.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Monitoring. 91.230 Section 91.230 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

24 CFR 91.230 - Monitoring.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Monitoring. 91.230 Section 91.230 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

14 CFR 91.1 - Applicability.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Applicability. 91.1 Section 91.1 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... prescribes rules governing the operation of aircraft (other than moored balloons, kites, unmanned rockets...

14 CFR 91.1 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Applicability. 91.1 Section 91.1 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... prescribes rules governing the operation of aircraft (other than moored balloons, kites, unmanned rockets...

15 CFR 922.91 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Definitions. 922.91 Section 922.91 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE OCEAN AND COASTAL RESOURCE MANAGEMENT NATIONAL...

9 CFR 381.91 - Contamination.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Contamination. 381.91 Section 381.91 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY... CERTIFICATION POULTRY PRODUCTS INSPECTION REGULATIONS Post Mortem Inspection; Disposition of Carcasses and Parts...

49 CFR 91.11 - Standards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Standards. 91.11 Section 91.11 Transportation Office of the Secretary of Transportation INTERNATIONAL AIR TRANSPORTATION FAIR COMPETITIVE PRACTICES.... flag air carriers and on foreign air carriers, estimates and periodic adjustments will be used to...

45 CFR 9.1 - Purpose.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 1 2013-10-01 2013-10-01 false Purpose. 9.1 Section 9.1 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION USE OF HHS RESEARCH FACILITIES BY ACADEMIC... and study facilities to academic scientists, engineers, and qualified students. ...

45 CFR 9.1 - Purpose.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 1 2014-10-01 2014-10-01 false Purpose. 9.1 Section 9.1 Public Welfare Department of Health and Human Services GENERAL ADMINISTRATION USE OF HHS RESEARCH FACILITIES BY ACADEMIC... and study facilities to academic scientists, engineers, and qualified students. ...

45 CFR 9.1 - Purpose.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 45 Public Welfare 1 2012-10-01 2012-10-01 false Purpose. 9.1 Section 9.1 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION USE OF HHS RESEARCH FACILITIES BY ACADEMIC... and study facilities to academic scientists, engineers, and qualified students. ...

Effect of mechanical vibrations on the wear behavior of AZ91 Mg alloy

NASA Astrophysics Data System (ADS)

Chaturvedi, V.; Pandel, U.; Sharma, A.

2018-02-01

AZ91 Mg alloy is the most promising alloy used for structural applications. The vibration induced methods are effective and economic viable in term of mechanical properties. Sliding wear tests were performed on AZ91 Mg alloy using a pin-on- disc configuration. Wear rates were measured at 5 N and 10N at a sliding velocity of 1m/s for varied frequency within the range of 5- 25Hz and a constant amplitude of 2mm. Microstructures of worn surfaces and wear debris were characterized by field emission scanning electron microscopy (FESEM). It is observed that wear resistance of vibrated AZ91 alloy at 15Hz frequency ad 2mm amplitude was superior than cast AZ91 Mg alloy. Finer grain size and equiaxed grain shape both are important parameters for better wear resistance in vibrated AZ91 Mg alloys. FESEM analysis revealed that wear is considerably affected due to frictional heat generated by the relative motion between AZ91 Mg alloy and EN31 steel surface. No single mechanism was responsible for material loss.

OSSOS. IX. Two Objects in Neptune's 9:1 Resonance—Implications for Resonance Sticking in the Scattering Population

NASA Astrophysics Data System (ADS)

Volk, Kathryn; Murray-Clay, Ruth A.; Gladman, Brett J.; Lawler, Samantha M.; Yu, Tze Yeung Mathew; Alexandersen, Mike; Bannister, Michele T.; Chen, Ying-Tung; Dawson, Rebekah I.; Greenstreet, Sarah; Gwyn, Stephen D. J.; Kavelaars, J. J.; Lin, Hsing Wen; Lykawka, Patryk Sofia; Petit, Jean-Marc

2018-06-01

We discuss the detection in the Outer Solar System Origins Survey (OSSOS) of two objects in Neptune’s distant 9:1 mean motion resonance at semimajor axis a ≈ 130 au. Both objects are securely resonant on 10 Myr timescales, with one securely in the 9:1 resonance’s leading asymmetric libration island and the other in either the symmetric or trailing asymmetric island. These objects are the largest semimajor axis objects with secure resonant classifications, and their detection in a carefully characterized survey allows for the first robust resonance population estimate beyond 100 au. The detection of these objects implies a 9:1 resonance population of 1.1 × 104 objects with H r < 8.66 (D ≳ 100 km) on similar orbits (95% confidence range of ∼(0.4–3) × 104). Integrations over 4 Gyr of an ensemble of clones spanning these objects’ orbit-fit uncertainties reveal that they both have median resonance occupation timescales of ∼1 Gyr. These timescales are consistent with the hypothesis that these objects originate in the scattering population but became transiently stuck to Neptune’s 9:1 resonance within the last ∼1 Gyr of solar system evolution. Based on simulations of a model of the current scattering population, we estimate the expected resonance sticking population in the 9:1 resonance to be 1000–4500 objects with H r < 8.66 this is marginally consistent with the OSSOS 9:1 population estimate. We conclude that resonance sticking is a plausible explanation for the observed 9:1 population, but we also discuss the possibility of a primordial 9:1 population, which would have interesting implications for the Kuiper Belt’s dynamical history.

40 CFR 91.1301 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES In-Use Credit Program for New Marine Engines § 91.1301 Applicability. Marine SI engines subject to the provisions of subpart A of this part 91 are eligible to...

18 CFR 4.91 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-04-01

... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false [Reserved] 4.91 Section 4.91 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT LICENSES, PERMITS, EXEMPTIONS, AND DETERMINATION OF...

18 CFR 4.91 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-04-01

... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false [Reserved] 4.91 Section 4.91 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT LICENSES, PERMITS, EXEMPTIONS, AND DETERMINATION OF...

18 CFR 4.91 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-04-01

... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false [Reserved] 4.91 Section 4.91 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT LICENSES, PERMITS, EXEMPTIONS, AND DETERMINATION OF...

18 CFR 4.91 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-04-01

... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false [Reserved] 4.91 Section 4.91 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT LICENSES, PERMITS, EXEMPTIONS, AND DETERMINATION OF...

18 CFR 4.91 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false [Reserved] 4.91 Section 4.91 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT LICENSES, PERMITS, EXEMPTIONS, AND DETERMINATION OF...

9 CFR 91.1 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Definitions. 91.1 Section 91.1 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF...

9 CFR 91.1 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Definitions. 91.1 Section 91.1 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF...

9 CFR 91.1 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Definitions. 91.1 Section 91.1 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF...

40 CFR 91.205 - Banking.

Code of Federal Regulations, 2010 CFR

2010-07-01

... three model years. Negative credits must be banked according to the requirements under § 91.207(c... negative credits under § 91.207 generates positive emission credits, when the following formula is used for... requirements of this part. Hydrocarbon Plus Oxides of Nitrogen Exhaust Emission Standards [Grams per kilowatt...

40 CFR 91.1002 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

40 CFR 91.1002 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

40 CFR 91.1002 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

40 CFR 91.1002 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

21 CFR 640.91 - Processing.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Processing. 640.91 Section 640.91 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) BIOLOGICS ADDITIONAL... intravenous injection. (c) Microbial contamination. All processing steps shall be conducted in a manner to...

9 CFR 91.6 - Goats.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Goats. 91.6 Section 91.6 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF LIVESTOCK FOR...

9 CFR 91.6 - Goats.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Goats. 91.6 Section 91.6 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF LIVESTOCK FOR...

9 CFR 91.6 - Goats.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Goats. 91.6 Section 91.6 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF LIVESTOCK FOR...

Chemically-induced Mouse Lung Tumors: Applications to ...

EPA Pesticide Factsheets

A state-of-the-science workshop on chemically-induced mouse lung tumors was conducted by U.S. Environmental Protection Agency to better understand the mouse lung tumor data’s role in human health assessments. Three environmental chemicals - naphthalene, styrene, and ethylbenzene were chosen for the analysis due to the commonality of mouse lung tumors in all three chemicals. The goals of the workshop were to: identify the evidence, from multiple scientific disciplines, regarding formation of chemically-induced lung tumors in mice; discuss analysis and interpretation of the evidence; discuss how such evidence informs human health assessments; and identify commonalities, linkages, or differences between the evidence from various disciplines and across the chemicals. Evidence informing the association between occupational exposure to styrene, ethylbenzene, or naphthalene and lung cancer; comparative biology of mouse lung tumors, associated pathologic effects, issues related to tissue and species concordance; mode of action analysis and biological mechanisms including pharmacokinetics and pharmacodynamics; and evidence from cellular, genetic and molecular toxicity was discussed. In summary, although consensus was not sought, the panelists agreed that available mouse lung tumor data should be considered for human health risk evaluation on an individual chemical basis. Key data gaps were identified that would assist in further understanding the mechanism and relevan

10 CFR 455.91 - Eligibility.

Code of Federal Regulations, 2010 CFR

2010-01-01

... part to fund, from non-Federal sources, energy conservation measures for eligible institutions; and (c... 10 Energy 3 2010-01-01 2010-01-01 false Eligibility. 455.91 Section 455.91 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION GRANT PROGRAMS FOR SCHOOLS AND HOSPITALS AND BUILDINGS OWNED BY UNITS OF LOCAL...

9 CFR 91.9 - Swine.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Swine. 91.9 Section 91.9 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND... export shipment, have been maintained isolated from the swine to be exported. All breeding swine shall be...

9 CFR 91.29 - Hatches.

Code of Federal Regulations, 2010 CFR

2010-01-01

... animal carcasses. Such hatches shall be watertight. [42 FR 28990, June 7, 1977. Redesignated at 45 FR... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Hatches. 91.29 Section 91.29 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION...

7 CFR 1000.91-1000.92 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 9 2010-01-01 2009-01-01 true [Reserved] 1000.91-1000.92 Section 1000.91-1000.92 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... Miscellaneous Provisions §§ 1000.91-1000.92 [Reserved] ...

32 CFR 935.91 - Summons.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 6 2012-07-01 2012-07-01 false Summons. 935.91 Section 935.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND... all books, papers, and witnesses needed by you to establish any defense you have to said claim. You...

32 CFR 935.91 - Summons.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 6 2013-07-01 2013-07-01 false Summons. 935.91 Section 935.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND... all books, papers, and witnesses needed by you to establish any defense you have to said claim. You...

32 CFR 935.91 - Summons.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 6 2014-07-01 2014-07-01 false Summons. 935.91 Section 935.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND... all books, papers, and witnesses needed by you to establish any defense you have to said claim. You...

32 CFR 935.91 - Summons.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 6 2011-07-01 2011-07-01 false Summons. 935.91 Section 935.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND... all books, papers, and witnesses needed by you to establish any defense you have to said claim. You...

32 CFR 935.91 - Summons.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 6 2010-07-01 2010-07-01 false Summons. 935.91 Section 935.91 National Defense Department of Defense (Continued) DEPARTMENT OF THE AIR FORCE TERRITORIAL AND INSULAR REGULATIONS WAKE ISLAND... all books, papers, and witnesses needed by you to establish any defense you have to said claim. You...

Marine bacteria from Danish coastal waters show antifouling activity against the marine fouling bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis independent of bacteriocidal activity.

PubMed

Bernbom, Nete; Ng, Yoke Yin; Kjelleberg, Staffan; Harder, Tilmann; Gram, Lone

2011-12-01

The aims of this study were to determine if marine bacteria from Danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. We further assess if antibacterial effect is a good proxy for antifouling activity. We isolated 110 bacteria with anti-Vibrio activity from different sample types and locations during a 1-year sampling from Danish coastal waters. The strains were identified as Pseudoalteromonas, Phaeobacter, and Vibrionaceae based on phenotypic tests and partial 16S rRNA gene sequence similarity. The numbers of bioactive bacteria were significantly higher in warmer than in colder months. While some species were isolated at all sampling locations, others were niche specific. We repeatedly isolated Phaeobacter gallaeciensis at surfaces from one site and Pseudoalteromonas tunicata at two others. Twenty-two strains, representing the major taxonomic groups, different seasons, and isolation strategies, were tested for antiadhesive effect against the marine biofilm-forming bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis. The antiadhesive effects were assessed by quantifying the number of strain S91 or Ulva spores attaching to a preformed biofilm of each of the 22 strains. The strongest antifouling activity was found in Pseudoalteromonas strains. Biofilms of Pseudoalteromonas piscicida, Pseudoalteromonas tunicata, and Pseudoalteromonas ulvae prevented Pseudoalteromonas S91 from attaching to steel surfaces. P. piscicida killed S91 bacteria in the suspension cultures, whereas P. tunicata and P. ulvae did not; however, they did prevent adhesion by nonbactericidal mechanism(s). Seven Pseudoalteromonas species, including P. piscicida and P. tunicata, reduced the number of settling Ulva zoospores to less than 10% of the number settling on control surfaces. The antifouling alpP gene was detected only in P. tunicata strains (with purple and yellow pigmentation), so

42 CFR 102.91 - Secretary's review authority.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 1 2012-10-01 2012-10-01 false Secretary's review authority. 102.91 Section 102.91 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Reconsideration of the Secretary's Determinations § 102.91 Secretary's review authority...

42 CFR 102.91 - Secretary's review authority.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Secretary's review authority. 102.91 Section 102.91 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Reconsideration of the Secretary's Determinations § 102.91 Secretary's review authority...

42 CFR 102.91 - Secretary's review authority.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 1 2011-10-01 2011-10-01 false Secretary's review authority. 102.91 Section 102.91 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Reconsideration of the Secretary's Determinations § 102.91 Secretary's review authority...

42 CFR 102.91 - Secretary's review authority.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 1 2013-10-01 2013-10-01 false Secretary's review authority. 102.91 Section 102.91 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Reconsideration of the Secretary's Determinations § 102.91 Secretary's review authority...

42 CFR 102.91 - Secretary's review authority.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 1 2014-10-01 2014-10-01 false Secretary's review authority. 102.91 Section 102.91 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Reconsideration of the Secretary's Determinations § 102.91 Secretary's review authority...

7 CFR 91.36 - Appeal laboratory certificate.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false Appeal laboratory certificate. 91.36 Section 91.36..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) COMMODITY LABORATORY TESTING PROGRAMS SERVICES AND GENERAL INFORMATION Appeal of Laboratory Services § 91.36 Appeal laboratory certificate. (a...

7 CFR 91.36 - Appeal laboratory certificate.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 3 2011-01-01 2011-01-01 false Appeal laboratory certificate. 91.36 Section 91.36..., Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) COMMODITY LABORATORY TESTING PROGRAMS SERVICES AND GENERAL INFORMATION Appeal of Laboratory Services § 91.36 Appeal laboratory certificate. (a...

Surface characterization and cytocompatibility evaluation of silanized magnesium alloy AZ91 for biomedical applications

NASA Astrophysics Data System (ADS)

Witecka, Agnieszka; Yamamoto, Akiko; Dybiec, Henryk; Swieszkowski, Wojciech

2012-12-01

Mg alloys with high Al contents have superior corrosion resistance in aqueous environments, but poor cytocompatibility compared to that of pure Mg. We have silanized the cast AZ91 alloy to improve its cytocompatibility using five different silanes: ethyltriethoxysilane (S1), 3-aminopropyltriethoxysilane (S2), 3-isocyanatopyltriethoxysilane (S3), phenyltriethoxysilane (S4) and octadecyltriethoxysilane (S5). The surface hydrophilicity/hydrophobicity was evaluated by water contact angle measurements. X-ray photoelectron analysis was performed to investigate the changes in surface states and chemical composition. All silane reagents increased adsorption of the albumin to the modified surface. In vitro cytocompatibility evaluation revealed that silanization improved cell growth on AZ91 modified by silane S1. Measurement of the concentration of Mg2+ ions released during the cell culture indicated that silanization does not affect substrate degradation.

49 CFR 229.91 - Motors and generators.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Motors and generators. 229.91 Section 229.91 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION....91 Motors and generators. A motor or a generator may not have any of the following conditions: (a) Be...

14 CFR 151.91 - Removal of obstructions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Removal of obstructions. 151.91 Section 151.91 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.91 Removal of obstructions. (a) The...

14 CFR 151.91 - Removal of obstructions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Removal of obstructions. 151.91 Section 151.91 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.91 Removal of obstructions. (a) The...

24 CFR 91.10 - Consolidated program year.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Consolidated program year. 91.10 Section 91.10 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS General § 91.10...

14 CFR 151.91 - Removal of obstructions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Removal of obstructions. 151.91 Section 151.91 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.91 Removal of obstructions. (a) The...

7 CFR 1735.91 - Location of facilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false Location of facilities. 1735.91 Section 1735.91 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF... All Acquisitions and Mergers § 1735.91 Location of facilities. Telephone facilities to be acquired...

40 CFR 91.303 - Acronyms and abbreviations.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Acronyms and abbreviations. 91.303 Section 91.303 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS....303 Acronyms and abbreviations. (a) The acronyms and abbreviations in § 91.5 apply to this subpart. (b...

40 CFR 91.303 - Acronyms and abbreviations.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Acronyms and abbreviations. 91.303 Section 91.303 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS....303 Acronyms and abbreviations. (a) The acronyms and abbreviations in § 91.5 apply to this subpart. (b...

14 CFR 91.869 - Carry-forward compliance.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Carry-forward compliance. 91.869 Section 91.869 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED....869 Carry-forward compliance. (a) Any operator that exceeds the requirements of paragraph (b) of § 91...

14 CFR 91.869 - Carry-forward compliance.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Carry-forward compliance. 91.869 Section 91.869 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED....869 Carry-forward compliance. (a) Any operator that exceeds the requirements of paragraph (b) of § 91...

14 CFR 151.91 - Removal of obstructions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Removal of obstructions. 151.91 Section 151.91 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS Project Programming Standards § 151.91 Removal of obstructions. (a) The...

7 CFR 1948.91 - Inspections of development.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 13 2011-01-01 2009-01-01 true Inspections of development. 1948.91 Section 1948.91...) PROGRAM REGULATIONS (CONTINUED) RURAL DEVELOPMENT Section 601 Energy Impacted Area Development Assistance Program § 1948.91 Inspections of development. Inspections will be made by the FmHA or its successor agency...

14 CFR 91.1061 - Augmented flight crews.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Augmented flight crews. 91.1061 Section 91...) AIR TRAFFIC AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Fractional Ownership Operations Program Management § 91.1061 Augmented flight crews. (a) No program manager may assign any flight...

14 CFR 91.515 - Flight altitude rules.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Flight altitude rules. 91.515 Section 91...) AIR TRAFFIC AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Large and Turbine-Powered Multiengine Airplanes and Fractional Ownership Program Aircraft § 91.515 Flight altitude rules. (a...

14 CFR 91.1061 - Augmented flight crews.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Augmented flight crews. 91.1061 Section 91...) AIR TRAFFIC AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Fractional Ownership Operations Program Management § 91.1061 Augmented flight crews. (a) No program manager may assign any flight...

A role for γS-crystallin in the organization of actin and fiber cell maturation in the mouse lens

PubMed Central

Fan, Jianguo; Dong, Lijin; Mishra, Sanghamitra; Chen, Yingwei; FitzGerald, Paul; Wistow, Graeme

2012-01-01

γS-crystallin (γS) is a highly conserved component of the eye lens. To gain insights into the functional role(s) of this protein, the mouse gene (Crygs) was deleted. Although mutations in γS can cause severe cataracts, loss of function of γS in knockout (KO) mice produced no obvious lens opacity, but was associated with focusing defects. Electron microscopy showed no major differences in lens cell organization, suggesting that the optical defects are primarily cytoplasmic in origin. KO lenses were also grossly normal by light microscopy but showed evidence of incomplete clearance of cellular organelles in maturing fiber cells. Phalloidin labeling showed an unusual distribution of F-actin in a band of mature fiber cells in KO lenses, suggesting a defect in the organization or processing of the actin cytoskeleton. Indeed, in wild-type lenses, γS and F-actin colocalize along the fiber cell plasma membrane. Relative levels of F-actin and G-actin in wild-type and KO lenses were estimated from fluorescent staining profiles and from isolation of actin fractions from whole lenses. Both methods showed a twofold reduction in the F-actin/G-actin ratio in KO lenses, whereas no difference in tubulin organization was detected. In vitro experiments showed that recombinant mouse γS can directly stabilize F-actin. This suggests that γS may have a functional role related to actin, perhaps in ‘shepherding’ filaments to maintain the optical properties of the lens cytoplasm and normal fiber cell maturation. PMID:22715935

14 CFR 91.23 - Truth-in-leasing clause requirement in leases and conditional sales contracts.

Code of Federal Regulations, 2010 CFR

2010-01-01

... leases and conditional sales contracts. 91.23 Section 91.23 Aeronautics and Space FEDERAL AVIATION... sales contracts. (a) Except as provided in paragraph (b) of this section, the parties to a lease or contract of conditional sale involving a U.S.-registered large civil aircraft and entered into after...

STS-91 Commander Precourt talks to Cosmonauts Kondakova and Ryumin at SLF

NASA Technical Reports Server (NTRS)

1998-01-01

STS-91 Mission Commander Charles Precourt (left) talks to Elena V. Kondakova and her husband, Valery Ryumin, a cosmonaut with the Russian Space Agency (RSA) and STS-91 mission specialist, at Kennedy Space Center's Shuttle Landing Facility (SLF). The STS-91 crew had just arrived at the SLF aboard T-38 jets in preparation for launch. Kondakova, also a cosmonaut with the RSA, flew with Commander Precourt as a mission specialist on STS-84 which launched on May 15, 1997. STS-91 is scheduled to be launched on June 2 on Space Shuttle Discovery with a launch window opening around 6:10 p.m. EDT. The mission will feature the ninth Shuttle docking with the Russian Space Station Mir, the first Mir docking for Discovery, the conclusion of Phase I of the joint U.S.- Russian International Space Station Program, and the first flight of the new Space Shuttle super lightweight external tank. The STS-91 flight crew also includes Pilot Dominic Gorie and Mission Specialists Wendy B. Lawrence; Franklin Chang-Diaz, Ph.D.; and Janet Kavandi, Ph.D. Andrew Thomas, Ph.D., will be returning to Earth with the crew after living more than four months aboard Mir.

49 CFR 91.5 - Findings and recommendations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 1 2011-10-01 2011-10-01 false Findings and recommendations. 91.5 Section 91.5... PRACTICES § 91.5 Findings and recommendations. (a) Upon finding that a foreign government or entity imposes... review of the report and recommendations made under paragraph (b) of this section, that unreasonably...

7 CFR 301.91-2 - Regulated articles.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 5 2013-01-01 2013-01-01 false Regulated articles. 301.91-2 Section 301.91-2... Regulations § 301.91-2 Regulated articles. The following are regulated articles: (a) Logs, pulpwood, branches...) Any other product, article, or means of conveyance, of any character whatsoever, not covered by...

7 CFR 301.91-2 - Regulated articles.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 5 2010-01-01 2010-01-01 false Regulated articles. 301.91-2 Section 301.91-2... Regulations § 301.91-2 Regulated articles. The following are regulated articles: (a) Logs, pulpwood, branches...) Any other product, article, or means of conveyance, of any character whatsoever, not covered by...

7 CFR 301.91-2 - Regulated articles.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 5 2012-01-01 2012-01-01 false Regulated articles. 301.91-2 Section 301.91-2... Regulations § 301.91-2 Regulated articles. The following are regulated articles: (a) Logs, pulpwood, branches...) Any other product, article, or means of conveyance, of any character whatsoever, not covered by...

7 CFR 301.91-2 - Regulated articles.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 5 2011-01-01 2011-01-01 false Regulated articles. 301.91-2 Section 301.91-2... Regulations § 301.91-2 Regulated articles. The following are regulated articles: (a) Logs, pulpwood, branches...) Any other product, article, or means of conveyance, of any character whatsoever, not covered by...

7 CFR 301.91-2 - Regulated articles.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 5 2014-01-01 2014-01-01 false Regulated articles. 301.91-2 Section 301.91-2... Regulations § 301.91-2 Regulated articles. The following are regulated articles: (a) Logs, pulpwood, branches...) Any other product, article, or means of conveyance, of any character whatsoever, not covered by...

46 CFR 91.25-25 - Hull equipment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Hull equipment. 91.25-25 Section 91.25-25 Shipping COAST... CERTIFICATION Inspection for Certification § 91.25-25 Hull equipment. (a) At each inspection for certification and periodic inspection, the inspectors shall conduct the following tests and inspections of hull...

40 CFR 91.410 - Engine test cycle.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Engine test cycle. 91.410 Section 91...) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.410 Engine... in dynamometer operation tests of marine engines. (b) During each non-idle mode the specified speed...

18 CFR 401.91 - Scope of subpart.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Scope of subpart. 401.91 Section 401.91 Conservation of Power and Water Resources DELAWARE RIVER BASIN COMMISSION ADMINISTRATIVE MANUAL RULES OF PRACTICE AND PROCEDURE Penalties and Settlements in Lieu of Penalties § 401.91...

Generating and Purifying Fab Fragments from Human and Mouse IgG Using the Bacterial Enzymes IdeS, SpeB and Kgp.

PubMed

Sjögren, Jonathan; Andersson, Linda; Mejàre, Malin; Olsson, Fredrik

2017-01-01

Fab fragments are valuable research tools in various areas of science including applications in imaging, binding studies, removal of Fc-mediated effector functions, mass spectrometry, infection biology, and many others. The enzymatic tools for the generation of Fab fragments have been discovered through basic research within the field of molecular bacterial pathogenesis. Today, these enzymes are widely applied as research tools and in this chapter, we describe methodologies based on bacterial enzymes to generate Fab fragments from both human and mouse IgG. For all human IgG subclasses, the IdeS enzyme from Streptococcus pyogenes has been applied to generate F(ab')2 fragments that subsequently can be reduced under mild conditions to generate a homogenous pool of Fab' fragments. The enzyme Kgp from Porphyromonas gingivalis has been applied to generate intact Fab fragments from human IgG1 and the Fab fragments can be purified using a CH1-specific affinity resin. The SpeB protease, also from S. pyogenes, is able to digest mouse IgGs and has been applied to digest antibodies and Fab fragments can be purified on light chain affinity resins. In this chapter, we describe methodologies that can be used to obtain Fab fragments from human and mouse IgG using bacterial proteases.

25 CFR 91.9 - Inheritance of improvements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Inheritance of improvements. 91.9 Section 91.9 Indians..., OSAGE RESERVATION, OKLAHOMA § 91.9 Inheritance of improvements. (a) Upon the death of the owner of... of the county courts, State of Oklahoma, and shall be subject to inheritance or bequest in accordance...

14 CFR 91.305 - Flight test areas.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Flight test areas. 91.305 Section 91.305... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.305 Flight test areas. No person may flight test an aircraft except over open water, or sparsely populated...

14 CFR 91.305 - Flight test areas.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Flight test areas. 91.305 Section 91.305... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.305 Flight test areas. No person may flight test an aircraft except over open water, or sparsely populated...

14 CFR 91.305 - Flight test areas.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Flight test areas. 91.305 Section 91.305... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Special Flight Operations § 91.305 Flight test areas. No person may flight test an aircraft except over open water, or sparsely populated...

36 CFR 9.1 - Purpose and scope.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 36 Parks, Forests, and Public Property 1 2014-07-01 2014-07-01 false Purpose and scope. 9.1 Section 9.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT Mining and Mining Claims § 9.1 Purpose and scope. These regulations control all activities within...

36 CFR 9.1 - Purpose and scope.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Purpose and scope. 9.1 Section 9.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT Mining and Mining Claims § 9.1 Purpose and scope. These regulations control all activities within...

36 CFR 9.1 - Purpose and scope.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 36 Parks, Forests, and Public Property 1 2011-07-01 2011-07-01 false Purpose and scope. 9.1 Section 9.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT Mining and Mining Claims § 9.1 Purpose and scope. These regulations control all activities within...

36 CFR 9.1 - Purpose and scope.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 36 Parks, Forests, and Public Property 1 2012-07-01 2012-07-01 false Purpose and scope. 9.1 Section 9.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT Mining and Mining Claims § 9.1 Purpose and scope. These regulations control all activities within...

36 CFR 9.1 - Purpose and scope.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 36 Parks, Forests, and Public Property 1 2013-07-01 2013-07-01 false Purpose and scope. 9.1 Section 9.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT Mining and Mining Claims § 9.1 Purpose and scope. These regulations control all activities within...

46 CFR 91.35-1 - When made.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false When made. 91.35-1 Section 91.35-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS INSPECTION AND CERTIFICATION Sanitary Inspections § 91.35-1 When made. (a) An inspection of quarters, toilet and washing spaces, serving...

46 CFR 91.35-1 - When made.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false When made. 91.35-1 Section 91.35-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS INSPECTION AND CERTIFICATION Sanitary Inspections § 91.35-1 When made. (a) An inspection of quarters, toilet and washing spaces, serving...

46 CFR 91.35-1 - When made.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false When made. 91.35-1 Section 91.35-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS INSPECTION AND CERTIFICATION Sanitary Inspections § 91.35-1 When made. (a) An inspection of quarters, toilet and washing spaces, serving...

46 CFR 91.35-1 - When made.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false When made. 91.35-1 Section 91.35-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS INSPECTION AND CERTIFICATION Sanitary Inspections § 91.35-1 When made. (a) An inspection of quarters, toilet and washing spaces, serving...

46 CFR 91.35-1 - When made.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false When made. 91.35-1 Section 91.35-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS INSPECTION AND CERTIFICATION Sanitary Inspections § 91.35-1 When made. (a) An inspection of quarters, toilet and washing spaces, serving...

31 CFR 91.13 - Weapons and explosives.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Weapons and explosives. 91.13 Section 91.13 Money and Finance: Treasury Regulations Relating to Money and Finance REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.13 Weapons and explosives. No person...

46 CFR 91.25-38 - Pollution prevention.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Pollution prevention. 91.25-38 Section 91.25-38 Shipping... CERTIFICATION Inspection for Certification § 91.25-38 Pollution prevention. At each inspection for certification... design and equipment requirements for pollution prevention in 33 CFR part 155, subpart B. [CGD 71-161R...

46 CFR 91.25-38 - Pollution prevention.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Pollution prevention. 91.25-38 Section 91.25-38 Shipping... CERTIFICATION Inspection for Certification § 91.25-38 Pollution prevention. At each inspection for certification... design and equipment requirements for pollution prevention in 33 CFR part 155, subpart B. [CGD 71-161R...

46 CFR 91.25-38 - Pollution prevention.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Pollution prevention. 91.25-38 Section 91.25-38 Shipping... CERTIFICATION Inspection for Certification § 91.25-38 Pollution prevention. At each inspection for certification... design and equipment requirements for pollution prevention in 33 CFR part 155, subpart B. [CGD 71-161R...

46 CFR 91.25-38 - Pollution prevention.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Pollution prevention. 91.25-38 Section 91.25-38 Shipping... CERTIFICATION Inspection for Certification § 91.25-38 Pollution prevention. At each inspection for certification... design and equipment requirements for pollution prevention in 33 CFR part 155, subpart B. [CGD 71-161R...

46 CFR 91.25-38 - Pollution prevention.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Pollution prevention. 91.25-38 Section 91.25-38 Shipping... CERTIFICATION Inspection for Certification § 91.25-38 Pollution prevention. At each inspection for certification... design and equipment requirements for pollution prevention in 33 CFR part 155, subpart B. [CGD 71-161R...

14 CFR 91.805 - Final compliance: Subsonic airplanes.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Final compliance: Subsonic airplanes. 91... § 91.805 Final compliance: Subsonic airplanes. Except as provided in §§ 91.809 and 91.811, on and after January 1, 1985, no person may operate to or from an airport in the United States any subsonic airplane...

14 CFR 91.805 - Final compliance: Subsonic airplanes.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Final compliance: Subsonic airplanes. 91... § 91.805 Final compliance: Subsonic airplanes. Except as provided in §§ 91.809 and 91.811, on and after January 1, 1985, no person may operate to or from an airport in the United States any subsonic airplane...

14 CFR 91.805 - Final compliance: Subsonic airplanes.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Final compliance: Subsonic airplanes. 91... § 91.805 Final compliance: Subsonic airplanes. Except as provided in §§ 91.809 and 91.811, on and after January 1, 1985, no person may operate to or from an airport in the United States any subsonic airplane...

14 CFR 91.805 - Final compliance: Subsonic airplanes.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Final compliance: Subsonic airplanes. 91... § 91.805 Final compliance: Subsonic airplanes. Except as provided in §§ 91.809 and 91.811, on and after January 1, 1985, no person may operate to or from an airport in the United States any subsonic airplane...

14 CFR 91.805 - Final compliance: Subsonic airplanes.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Final compliance: Subsonic airplanes. 91... § 91.805 Final compliance: Subsonic airplanes. Except as provided in §§ 91.809 and 91.811, on and after January 1, 1985, no person may operate to or from an airport in the United States any subsonic airplane...

40 CFR 91.409 - Engine dynamometer test run.

Code of Federal Regulations, 2014 CFR

2014-07-01

... all pre-test data specified in § 91.405(c). (4) Start the test cycle (see § 91.410) within 10 minutes... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Engine dynamometer test run. 91.409... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.409...

40 CFR 91.409 - Engine dynamometer test run.

Code of Federal Regulations, 2010 CFR

2010-07-01

... all pre-test data specified in § 91.405(c). (4) Start the test cycle (see § 91.410) within 10 minutes... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Engine dynamometer test run. 91.409... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.409...

40 CFR 91.409 - Engine dynamometer test run.

Code of Federal Regulations, 2013 CFR

2013-07-01

... all pre-test data specified in § 91.405(c). (4) Start the test cycle (see § 91.410) within 10 minutes... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Engine dynamometer test run. 91.409... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.409...

40 CFR 91.409 - Engine dynamometer test run.

Code of Federal Regulations, 2012 CFR

2012-07-01

... all pre-test data specified in § 91.405(c). (4) Start the test cycle (see § 91.410) within 10 minutes... 40 Protection of Environment 21 2012-07-01 2012-07-01 false Engine dynamometer test run. 91.409... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.409...

40 CFR 91.409 - Engine dynamometer test run.

Code of Federal Regulations, 2011 CFR

2011-07-01

... all pre-test data specified in § 91.405(c). (4) Start the test cycle (see § 91.410) within 10 minutes... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Engine dynamometer test run. 91.409... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Gaseous Exhaust Test Procedures § 91.409...

14 CFR 91.17 - Alcohol or drugs.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Alcohol or drugs. 91.17 Section 91.17... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES General § 91.17 Alcohol or drugs. (a... consumption of any alcoholic beverage; (2) While under the influence of alcohol; (3) While using any drug that...

14 CFR 91.17 - Alcohol or drugs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Alcohol or drugs. 91.17 Section 91.17... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES General § 91.17 Alcohol or drugs. (a... consumption of any alcoholic beverage; (2) While under the influence of alcohol; (3) While using any drug that...

14 CFR 91.17 - Alcohol or drugs.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Alcohol or drugs. 91.17 Section 91.17... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES General § 91.17 Alcohol or drugs. (a... consumption of any alcoholic beverage; (2) While under the influence of alcohol; (3) While using any drug that...

14 CFR 91.17 - Alcohol or drugs.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Alcohol or drugs. 91.17 Section 91.17... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES General § 91.17 Alcohol or drugs. (a... consumption of any alcoholic beverage; (2) While under the influence of alcohol; (3) While using any drug that...

14 CFR 91.125 - ATC light signals.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Flight Rules General § 91.125 ATC light signals. ATC light signals have the meaning shown in the following table: Color and type of signal Meaning... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false ATC light signals. 91.125 Section 91.125...

14 CFR 91.17 - Alcohol or drugs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Alcohol or drugs. 91.17 Section 91.17... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES General § 91.17 Alcohol or drugs. (a... consumption of any alcoholic beverage; (2) While under the influence of alcohol; (3) While using any drug that...

31 CFR 91.4 - Preservation of property.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Preservation of property. 91.4 Section 91.4 Money and Finance: Treasury Regulations Relating to Money and Finance REGULATIONS GOVERNING CONDUCT IN OR ON THE BUREAU OF THE MINT BUILDINGS AND GROUNDS § 91.4 Preservation of property. It shall be...

14 CFR 91.125 - ATC light signals.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false ATC light signals. 91.125 Section 91.125... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Flight Rules General § 91.125 ATC light signals. ATC light signals have the meaning shown in the following table: Color and type of signal Meaning...

14 CFR 91.125 - ATC light signals.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false ATC light signals. 91.125 Section 91.125... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Flight Rules General § 91.125 ATC light signals. ATC light signals have the meaning shown in the following table: Color and type of signal Meaning...

14 CFR 91.125 - ATC light signals.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false ATC light signals. 91.125 Section 91.125... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Flight Rules General § 91.125 ATC light signals. ATC light signals have the meaning shown in the following table: Color and type of signal Meaning...

14 CFR 91.125 - ATC light signals.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false ATC light signals. 91.125 Section 91.125... AND GENERAL OPERATING RULES GENERAL OPERATING AND FLIGHT RULES Flight Rules General § 91.125 ATC light signals. ATC light signals have the meaning shown in the following table: Color and type of signal Meaning...

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity▿†

PubMed Central

Bernbom, Nete; Ng, Yoke Yin; Kjelleberg, Staffan; Harder, Tilmann; Gram, Lone

2011-01-01

The aims of this study were to determine if marine bacteria from Danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. We further assess if antibacterial effect is a good proxy for antifouling activity. We isolated 110 bacteria with anti-Vibrio activity from different sample types and locations during a 1-year sampling from Danish coastal waters. The strains were identified as Pseudoalteromonas, Phaeobacter, and Vibrionaceae based on phenotypic tests and partial 16S rRNA gene sequence similarity. The numbers of bioactive bacteria were significantly higher in warmer than in colder months. While some species were isolated at all sampling locations, others were niche specific. We repeatedly isolated Phaeobacter gallaeciensis at surfaces from one site and Pseudoalteromonas tunicata at two others. Twenty-two strains, representing the major taxonomic groups, different seasons, and isolation strategies, were tested for antiadhesive effect against the marine biofilm-forming bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis. The antiadhesive effects were assessed by quantifying the number of strain S91 or Ulva spores attaching to a preformed biofilm of each of the 22 strains. The strongest antifouling activity was found in Pseudoalteromonas strains. Biofilms of Pseudoalteromonas piscicida, Pseudoalteromonas tunicata, and Pseudoalteromonas ulvae prevented Pseudoalteromonas S91 from attaching to steel surfaces. P. piscicida killed S91 bacteria in the suspension cultures, whereas P. tunicata and P. ulvae did not; however, they did prevent adhesion by nonbactericidal mechanism(s). Seven Pseudoalteromonas species, including P. piscicida and P. tunicata, reduced the number of settling Ulva zoospores to less than 10% of the number settling on control surfaces. The antifouling alpP gene was detected only in P. tunicata strains (with purple and yellow pigmentation), so

Surface characterization and cytocompatibility evaluation of silanized magnesium alloy AZ91 for biomedical applications

PubMed Central

Witecka, Agnieszka; Yamamoto, Akiko; Dybiec, Henryk; Swieszkowski, Wojciech

2012-01-01

Mg alloys with high Al contents have superior corrosion resistance in aqueous environments, but poor cytocompatibility compared to that of pure Mg. We have silanized the cast AZ91 alloy to improve its cytocompatibility using five different silanes: ethyltriethoxysilane (S1), 3-aminopropyltriethoxysilane (S2), 3-isocyanatopyltriethoxysilane (S3), phenyltriethoxysilane (S4) and octadecyltriethoxysilane (S5). The surface hydrophilicity/hydrophobicity was evaluated by water contact angle measurements. X-ray photoelectron analysis was performed to investigate the changes in surface states and chemical composition. All silane reagents increased adsorption of the albumin to the modified surface. In vitro cytocompatibility evaluation revealed that silanization improved cell growth on AZ91 modified by silane S1. Measurement of the concentration of Mg2+ ions released during the cell culture indicated that silanization does not affect substrate degradation. PMID:27877541

14 CFR 91.709 - Operations to Cuba.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Operations to Cuba. 91.709 Section 91.709... Board Such Aircraft § 91.709 Operations to Cuba. No person may operate a civil aircraft from the United States to Cuba unless— (a) Departure is from an international airport of entry designated in § 6.13 of...

14 CFR 91.709 - Operations to Cuba.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Operations to Cuba. 91.709 Section 91.709... Board Such Aircraft § 91.709 Operations to Cuba. No person may operate a civil aircraft from the United States to Cuba unless— (a) Departure is from an international airport of entry designated in § 6.13 of...

14 CFR 91.709 - Operations to Cuba.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Operations to Cuba. 91.709 Section 91.709... Board Such Aircraft § 91.709 Operations to Cuba. No person may operate a civil aircraft from the United States to Cuba unless— (a) Departure is from an international airport of entry designated in § 6.13 of...

14 CFR 91.709 - Operations to Cuba.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Operations to Cuba. 91.709 Section 91.709... Board Such Aircraft § 91.709 Operations to Cuba. No person may operate a civil aircraft from the United States to Cuba unless— (a) Departure is from an international airport of entry designated in § 6.13 of...

14 CFR 91.709 - Operations to Cuba.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Operations to Cuba. 91.709 Section 91.709... Board Such Aircraft § 91.709 Operations to Cuba. No person may operate a civil aircraft from the United States to Cuba unless— (a) Departure is from an international airport of entry designated in § 6.13 of...

31 CFR 50.91 - Notice to Congress.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Notice to Congress. 50.91 Section 50.91 Money and Finance: Treasury Office of the Secretary of the Treasury TERRORISM RISK INSURANCE PROGRAM Cap on Annual Liability § 50.91 Notice to Congress. Pursuant to section 103(e)(3) of the Act, the...

46 CFR 91.25-45 - Fire hazards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Fire hazards. 91.25-45 Section 91.25-45 Shipping COAST... CERTIFICATION Inspection for Certification § 91.25-45 Fire hazards. (a) At each inspection for certification and... that there is no accumulation of oil which might create a fire hazard. [CGFR 65-50, 30 FR 16974, Dec...

THE USE OF Y$sup 91$ AS AN INERT INDICATOR IN INTESTINAL ABSORPTION TESTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maisterrena, J.A.; Murphy, C.A.; Tovar, E.

The use of Y/sup 91/ as an inert-indicator in the studies of the intestinal absorption of I/sup 131/-labeled substances is reported, emphasis being given to its clinical advantages. The Y/sup 91/ is not absorbed in the gastrointestinal tract, since recovery in the feces in 22 cases was 96 plus or minus 6.7% (S.D.) of the amount given. The Y/sup 91/ and the I/sup 131/-labeled substances are homogeneously distributed throughout any given stool sample but their rate of excretion is not always parallel. A close correlation was found between the amount of I/sup 131/ excreted as obtained from the complete fecalmore » collection method and that of Y/sup 91/-indicator method in a group of 20 subjects under special supervision. The value of the Y/sup 91/ inert-indicator method where the completeness of the stool collection is doubtful is shown in 23 cases. (auth)« less

40 CFR 428.91 - Specialized definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 30 2014-07-01 2014-07-01 false Specialized definitions. 428.91 Section 428.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) RUBBER MANUFACTURING POINT SOURCE CATEGORY Pan, Dry Digestion, and...

40 CFR 428.91 - Specialized definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 31 2013-07-01 2013-07-01 false Specialized definitions. 428.91 Section 428.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) RUBBER MANUFACTURING POINT SOURCE CATEGORY Pan, Dry Digestion, and...

40 CFR 428.91 - Specialized definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 30 2011-07-01 2011-07-01 false Specialized definitions. 428.91 Section 428.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS RUBBER MANUFACTURING POINT SOURCE CATEGORY Pan, Dry Digestion, and Mechanical Reclaimed...

40 CFR 428.91 - Specialized definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 31 2012-07-01 2012-07-01 false Specialized definitions. 428.91 Section 428.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) RUBBER MANUFACTURING POINT SOURCE CATEGORY Pan, Dry Digestion, and...

40 CFR 417.91 - Specialized definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Specialized definitions. 417.91 Section 417.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum Sulfonation and Sulfation...

40 CFR 428.91 - Specialized definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Specialized definitions. 428.91 Section 428.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS RUBBER MANUFACTURING POINT SOURCE CATEGORY Pan, Dry Digestion, and Mechanical Reclaimed...

40 CFR 91.603 - Applicability of part 91, subpart F.

Code of Federal Regulations, 2014 CFR

2014-07-01

... violations of the Clean Air Act and the regulations thereunder. (Authorized Company Representative.) (9....603 Section 91.603 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... representative of the manufacturer: This report is submitted pursuant to Sections 213 and 208 of the Clean Air...

gp-91 mediates histone deacetylase inhibition-induced cardioprotection

PubMed Central

Zhao, Ting C; Zhang, Ling X; Cheng, Guangmao; Liu, Jun T

2010-01-01

We have recently shown that the inhibition of histone deacetylases (HDAC) protects the heart against ischemia and reperfusion (I/R) injury. The mechanism by which HDAC inhibition induces cardioprotection remains unknown. We sought to investigate whether the genetic disruption of gp-91, a subunit of NADPH-oxidase, would mitigate cardioprotection of HDAC inhibition. Wild-type and gp-91−/− mice were treated with a potent inhibitor of HDACs, trichostatin A (TSA, 0.1mg/kg, i.p.). Twenty-four hours later, the perfused hearts were subjected to 30 min of ischemia and 30 min of reperfusion. HDAC inhibition in wild-type mice produced marked improvements in ventricular functional recovery and the reduction of infarct size. TSA-induced cardioprotection was eliminated with genetic deletion of gp91. Notably, Western blot and immunostaining displayed a significant increase in gp-91 in myocardium following HDAC inhibition, which resulted in a mildly subsequent increase in the production of reactive oxygen species (ROS). The pretreatment of H9c2 cardiomyoblasts with TSA (50 nmol/L) decreased cell necrosis and increased viability in response to simulated ischemia (SI), which was abrogated by the transfection of cells with gp-91 siRNA, but not by scrambled siRNA. Furthermore, treatment of PLB-985 gp91+/+cells with TSA increased the resistance to SI, which also diminished with genetic disruption of gp91 in gp91phox-deficient PLB-985 cells. TSA treatment inhibited the increased active caspase-3 in H9c2 cardiomyoblasts and PLB-985 gp91+/+cells exposed to SI, which were prevented by knockdown of gp-91 by siRNA. These results suggest that a cascade consisting of gp-91 and HDAC inhibition plays an essential role in orchestrating the cardioprotective effect. PMID:20433879

40 CFR 417.91 - Specialized definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Specialized definitions. 417.91 Section 417.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum Sulfonation and Sulfation Subcategory...

40 CFR 417.91 - Specialized definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. 417.91 Section 417.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum Sulfonation and Sulfation Subcategory...

40 CFR 417.91 - Specialized definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. 417.91 Section 417.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum Sulfonation and Sulfation Subcategory...

40 CFR 417.91 - Specialized definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 417.91 Section 417.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Oleum Sulfonation and Sulfation Subcategory...

7 CFR 955.91 - Additional parties.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 8 2011-01-01 2011-01-01 false Additional parties. 955.91 Section 955.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE VIDALIA ONIONS GROWN IN GEORGIA...

7 CFR 955.91 - Additional parties.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 8 2014-01-01 2014-01-01 false Additional parties. 955.91 Section 955.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE VIDALIA ONIONS GROWN IN GEORGIA...

7 CFR 955.91 - Additional parties.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Additional parties. 955.91 Section 955.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE VIDALIA ONIONS GROWN IN GEORGIA...

7 CFR 955.91 - Additional parties.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 8 2013-01-01 2013-01-01 false Additional parties. 955.91 Section 955.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE VIDALIA ONIONS GROWN IN GEORGIA...

7 CFR 955.91 - Additional parties.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 8 2012-01-01 2012-01-01 false Additional parties. 955.91 Section 955.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE VIDALIA ONIONS GROWN IN GEORGIA...

14 CFR 91.1015 - Management specifications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Management specifications. 91.1015 Section... Operations Program Management § 91.1015 Management specifications. (a) Each person conducting operations under this subpart or furnishing fractional ownership program management services to fractional owners...

40 CFR 408.91 - Specialized definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 408.91 Section 408.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Non-Remote Alaskan Shrimp Processing...

40 CFR 408.91 - Specialized definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Specialized definitions. 408.91 Section 408.91 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY Non-Remote Alaskan Shrimp Processing...

Olfaction variation in mouse husbandry and its implications for refinement and standardization: UK survey of animal scents.

PubMed

López-Salesansky, Noelia; Mazlan, Nur H; Whitfield, Lucy E; Wells, Dominic J; Burn, Charlotte C

2016-10-01

Olfaction plays a crucial role in mouse communication, providing information about genetic identity, physiological status of conspecifics and alerting mice to potential predators. Scents of animal origin can trigger physiological and behavioural responses that could affect experimental responses and impact positively or negatively on mouse welfare. Additionally, differing olfactory profiles could help explain variation in results between laboratories. A survey was sent to animal research units in the UK to investigate potential transfer of scents of animal origin during routine husbandry procedures, and responses were obtained from animal care workers and researchers using mice in 51 institutions. The results reveal great diversity between animal units regarding the relevant husbandry routines covered. Most [71%] reported housing non-breeding male and female mice in the same room, with 76% reporting that hands were not washed and gloves not changed between handling male and female mice. The most commonly reported species housed in the same facility as mice was the rat (91%), and 41% of respondents were aware that scents from rats could affect mice. Changing of gloves between handling mice and other species was reported by 79% of respondents. Depending on the aspect considered, between 18 and 33% of respondents believed human and non-human animal odours would strongly affect mouse physiology, behaviour or standardization, while approximately 32-54% believed these effects would be weak. This indicates uncertainty regarding the significance of these factors. Understanding and controlling these practices could reduce unwanted variability in experimental results and maximize welfare. © The Author(s) 2015.

24 CFR 91.110 - Consultation; states.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Consultation; states. 91.110 Section 91.110 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Citizen Participation and...

27 CFR 479.91 - Unserviceable firearms.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 27 Alcohol, Tobacco Products and Firearms 3 2013-04-01 2013-04-01 false Unserviceable firearms. 479.91 Section 479.91 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND...

27 CFR 479.91 - Unserviceable firearms.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 3 2011-04-01 2010-04-01 true Unserviceable firearms. 479.91 Section 479.91 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN...

27 CFR 479.91 - Unserviceable firearms.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 27 Alcohol, Tobacco Products and Firearms 3 2012-04-01 2010-04-01 true Unserviceable firearms. 479.91 Section 479.91 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN...

27 CFR 479.91 - Unserviceable firearms.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 27 Alcohol, Tobacco Products and Firearms 3 2014-04-01 2014-04-01 false Unserviceable firearms. 479.91 Section 479.91 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND...

27 CFR 479.91 - Unserviceable firearms.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false Unserviceable firearms. 479.91 Section 479.91 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND...

Metabolic and Blood Pressure Effects of Walnut Supplementation in a Mouse Model of the Metabolic Syndrome.

PubMed

Scott, Nicola J A; Ellmers, Leigh J; Pilbrow, Anna P; Thomsen, Lotte; Richards, Arthur Mark; Frampton, Chris M; Cameron, Vicky A

2017-07-07

There is extensive evidence that walnut consumption is protective against cardiovascular disease and diabetes in the healthy population, but the beneficial effects of walnut consumption in individuals with the metabolic syndrome (MetS) remain uncertain. We compared a range of cardio-metabolic traits and related tissue gene expression associated with 21 weeks of dietary walnut supplementation in a mouse model of MetS (MetS-Tg) and wild-type (WT) mice ( n = 10 per genotype per diet, equal males and females). Compared to standard diet, walnuts did not significantly alter food consumption or body weight trajectory of either MetS-Tg or WT mice. In MetS-Tg mice, walnuts were associated with reductions in oral glucose area under the curve (gAUC, standard diet 1455 ± 54, walnut 1146 ± 91, p = 0.006) and mean arterial blood pressure (MAP, standard diet 100.6 ± 1.9, walnut 73.2 ± 1.8 mmHg, p < 0.001), with neutral effects on gAUC and MAP in WT mice. However, in MetS-Tg mice, walnuts were also associated with trends for higher plasma cholesterol (standard diet 4.73 ± 0.18, walnut 7.03 ± 1.99 mmol/L, p = 0.140) and triglyceride levels (standard diet 2.4 ± 0.5, walnut 5.4 ± 1.6 mmol/L, p = 0.061), despite lowering cholesterol and having no effect on triglycerides in WT mice. Moreover, in both MetS-Tg and WT mice, walnuts were associated with significantly increased liver expression of genes associated with metabolism ( Fabp1 , Insr ), cell stress ( Atf6 , Ddit3 , Eif2ak3 ), fibrosis ( Hgf , Sp1 , Timp1 ) and inflammation ( Tnf , Ptpn22 , Pparg ). In conclusion, dietary walnuts were associated with modest favourable effects in WT mice, but a combination of beneficial and adverse effects in MetS-Tg mice, and up-regulation of hepatic pro-fibrotic and pro-inflammatory genes in both mouse strains.

Mouse IDGenes: a reference database for genetic interactions in the developing mouse brain

PubMed Central

Matthes, Michaela; Preusse, Martin; Zhang, Jingzhong; Schechter, Julia; Mayer, Daniela; Lentes, Bernd; Theis, Fabian; Prakash, Nilima; Wurst, Wolfgang; Trümbach, Dietrich

2014-01-01

The study of developmental processes in the mouse and other vertebrates includes the understanding of patterning along the anterior–posterior, dorsal–ventral and medial– lateral axis. Specifically, neural development is also of great clinical relevance because several human neuropsychiatric disorders such as schizophrenia, autism disorders or drug addiction and also brain malformations are thought to have neurodevelopmental origins, i.e. pathogenesis initiates during childhood and adolescence. Impacts during early neurodevelopment might also predispose to late-onset neurodegenerative disorders, such as Parkinson’s disease. The neural tube develops from its precursor tissue, the neural plate, in a patterning process that is determined by compartmentalization into morphogenetic units, the action of local signaling centers and a well-defined and locally restricted expression of genes and their interactions. While public databases provide gene expression data with spatio-temporal resolution, they usually neglect the genetic interactions that govern neural development. Here, we introduce Mouse IDGenes, a reference database for genetic interactions in the developing mouse brain. The database is highly curated and offers detailed information about gene expressions and the genetic interactions at the developing mid-/hindbrain boundary. To showcase the predictive power of interaction data, we infer new Wnt/β-catenin target genes by machine learning and validate one of them experimentally. The database is updated regularly. Moreover, it can easily be extended by the research community. Mouse IDGenes will contribute as an important resource to the research on mouse brain development, not exclusively by offering data retrieval, but also by allowing data input. Database URL: http://mouseidgenes.helmholtz-muenchen.de. PMID:25145340

14 CFR 91.1107 - Recurrent training.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Recurrent training. 91.1107 Section 91.1107 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... quiz or other review to determine the crewmember's knowledge of the aircraft and crewmember position...

14 CFR 91.1107 - Recurrent training.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Recurrent training. 91.1107 Section 91.1107 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... quiz or other review to determine the crewmember's knowledge of the aircraft and crewmember position...

24 CFR 91.215 - Strategic plan.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Strategic plan. 91.215 Section 91.215 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

24 CFR 91.215 - Strategic plan.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Strategic plan. 91.215 Section 91.215 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

24 CFR 91.215 - Strategic plan.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Strategic plan. 91.215 Section 91.215 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development CONSOLIDATED SUBMISSIONS FOR COMMUNITY PLANNING AND DEVELOPMENT PROGRAMS Local Governments; Contents of...

14 CFR 91.1002 - Compliance date.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Compliance date. 91.1002 Section 91.1002 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC....1002 Compliance date. No person that conducted flights before November 17, 2003 under a program that...

14 CFR 91.103 - Preflight action.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Preflight action. 91.103 Section 91.103 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... action. Each pilot in command shall, before beginning a flight, become familiar with all available...

14 CFR 91.103 - Preflight action.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Preflight action. 91.103 Section 91.103 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... action. Each pilot in command shall, before beginning a flight, become familiar with all available...

9 CFR 91.7 - Captive cervids.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Captive cervids. 91.7 Section 91.7 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF...

40 CFR 280.91 - Compliance dates.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 27 2011-07-01 2011-07-01 false Compliance dates. 280.91 Section 280... (UST) Financial Responsibility § 280.91 Compliance dates. Owners of petroleum underground storage tanks are required to comply with the requirements of this subpart by the following dates: (a) All petroleum...

50 CFR 18.91 - Director's decision.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Director's decision. 18.91 Section 18.91...) TAKING, POSSESSION, TRANSPORTATION, SALE, PURCHASE, BARTER, EXPORTATION, AND IMPORTATION OF WILDLIFE AND... decision. (a) Upon receipt of the recommended decision and transcript and after the thirty-day period for...

14 CFR 91.905 - List of rules subject to waivers.

Code of Federal Regulations, 2010 CFR

2010-01-01

....113Right-of-way rules: Except water operations. 91.115Right-of-way rules: Water operations. 91.117Aircraft speed. 91.119Minimum safe altitudes: General. 91.121Altimeter settings. 91.123Compliance with ATC... between Mexico or Canada and the United States. 91.713Operation of civil aircraft of Cuban registry. [Doc...

14 CFR 91.905 - List of rules subject to waivers.

Code of Federal Regulations, 2011 CFR

2011-01-01

....113Right-of-way rules: Except water operations. 91.115Right-of-way rules: Water operations. 91.117Aircraft speed. 91.119Minimum safe altitudes: General. 91.121Altimeter settings. 91.123Compliance with ATC... between Mexico or Canada and the United States. 91.713Operation of civil aircraft of Cuban registry. [Doc...

14 CFR 91.1085 - Hazardous materials recognition training.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Hazardous materials recognition training. 91.1085 Section 91.1085 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Ownership Operations Program Management § 91.1085 Hazardous materials recognition training. No program...

14 CFR 91.1085 - Hazardous materials recognition training.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Hazardous materials recognition training. 91.1085 Section 91.1085 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Ownership Operations Program Management § 91.1085 Hazardous materials recognition training. No program...

14 CFR 91.1085 - Hazardous materials recognition training.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Hazardous materials recognition training. 91.1085 Section 91.1085 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Ownership Operations Program Management § 91.1085 Hazardous materials recognition training. No program...

14 CFR 91.1085 - Hazardous materials recognition training.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Hazardous materials recognition training. 91.1085 Section 91.1085 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Ownership Operations Program Management § 91.1085 Hazardous materials recognition training. No program...

14 CFR 91.1085 - Hazardous materials recognition training.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Hazardous materials recognition training. 91.1085 Section 91.1085 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... Ownership Operations Program Management § 91.1085 Hazardous materials recognition training. No program...

Centralized mouse repositories.

PubMed

Donahue, Leah Rae; Hrabe de Angelis, Martin; Hagn, Michael; Franklin, Craig; Lloyd, K C Kent; Magnuson, Terry; McKerlie, Colin; Nakagata, Naomi; Obata, Yuichi; Read, Stuart; Wurst, Wolfgang; Hörlein, Andreas; Davisson, Muriel T

2012-10-01

Because the mouse is used so widely for biomedical research and the number of mouse models being generated is increasing rapidly, centralized repositories are essential if the valuable mouse strains and models that have been developed are to be securely preserved and fully exploited. Ensuring the ongoing availability of these mouse strains preserves the investment made in creating and characterizing them and creates a global resource of enormous value. The establishment of centralized mouse repositories around the world for distributing and archiving these resources has provided critical access to and preservation of these strains. This article describes the common and specialized activities provided by major mouse repositories around the world.

The MAGIC Touch: Combining MAGIC-Pointing with a Touch-Sensitive Mouse

NASA Astrophysics Data System (ADS)

Drewes, Heiko; Schmidt, Albrecht

In this paper, we show how to use the combination of eye-gaze and a touch-sensitive mouse to ease pointing tasks in graphical user interfaces. A touch of the mouse positions the mouse pointer at the current gaze position of the user. Thus, the pointer is always at the position where the user expects it on the screen. This approach changes the user experience in tasks that include frequent switching between keyboard and mouse input (e.g. working with spreadsheets). In a user study, we compared the touch-sensitive mouse with a traditional mouse and observed speed improvements for pointing tasks on complex backgrounds. For pointing task on plain backgrounds, performances with both devices were similar, but users perceived the gaze-sensitive interaction of the touch-sensitive mouse as being faster and more convenient. Our results show that using a touch-sensitive mouse that positions the pointer on the user’s gaze position reduces the need for mouse movements in pointing tasks enormously.

STS-91 Mission Specialist Kavandi visits Pad 39A before launch

NASA Technical Reports Server (NTRS)

1998-01-01

STS-91 Mission Specialist Janet Kavandi, Ph.D., visits Launch Pad 39A from which she is scheduled to be launched aboard Space Shuttle Discovery on June 2 around 6:10 p.m. EDT. In her pocket are flowers intended as gifts for her two children whom she will be seeing shortly. STS-91 will feature the ninth Shuttle docking with the Russian Space Station Mir, the first Mir docking for Discovery, the conclusion of Phase I of the joint U.S.-Russian International Space Station Program, and the first flight of the new Space Shuttle super lightweight external tank. The STS-91 flight crew also includes Commander Charles Precourt; Pilot Dominic Gorie; and Mission Specialists Franklin Chang-Diaz, Ph.D.; Wendy B. Lawrence; and Valery Ryumin, with the Russian Space Agency. Andrew Thomas, Ph.D., will be returning to Earth with the crew after living more than four months aboard Mir.

40 CFR 91.606 - Sample selection.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Sample selection. 91.606 Section 91....606 Sample selection. (a) Engines comprising a test sample will be selected at the location and in the... in the manner specified in the test order, an alternative selection procedure may be employed...

9 CFR 3.91 - Terminal facilities.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Terminal facilities. 3.91 Section 3.91 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL... animal holding area of a terminal facility must provide the following: (1) Shelter from sunlight and...

9 CFR 3.91 - Terminal facilities.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Terminal facilities. 3.91 Section 3.91 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL... animal holding area of a terminal facility must provide the following: (1) Shelter from sunlight and...

9 CFR 3.91 - Terminal facilities.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Terminal facilities. 3.91 Section 3.91 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL... animal holding area of a terminal facility must provide the following: (1) Shelter from sunlight and...

40 CFR 91.6 - Reference materials.

Code of Federal Regulations, 2014 CFR

2014-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES General § 91.6 Reference materials. (a) Incorporation by... the Research Method Appendix A to Subpart D. ASTM D2700-92: Standard Test Method for Knock... 40 CFR part 91 reference SAE J1228/ISO 8665 November 1991 Small Craft-Marine Propulsion Engine and...

40 CFR 91.6 - Reference materials.

Code of Federal Regulations, 2013 CFR

2013-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES General § 91.6 Reference materials. (a) Incorporation by... the Research Method Appendix A to Subpart D. ASTM D2700-92: Standard Test Method for Knock... 40 CFR part 91 reference SAE J1228/ISO 8665 November 1991 Small Craft-Marine Propulsion Engine and...

40 CFR 91.6 - Reference materials.

Code of Federal Regulations, 2012 CFR

2012-07-01

... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES General § 91.6 Reference materials. (a) Incorporation by... the Research Method Appendix A to Subpart D. ASTM D2700-92: Standard Test Method for Knock... 40 CFR part 91 reference SAE J1228/ISO 8665 November 1991 Small Craft-Marine Propulsion Engine and...

28 CFR 91.24 - Grant distribution.

Code of Federal Regulations, 2010 CFR

2010-07-01

... reserve, to carry out this program— (1) 0.3 percent in each fiscal years 1996 and 1997; and (2) 0.2... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Grant distribution. 91.24 Section 91.24 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) GRANTS FOR CORRECTIONAL FACILITIES Correctional...

24 CFR 91.520 - Performance reports.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Performance reports. 91.520 Section... Requirements § 91.520 Performance reports. (a) General. Each jurisdiction that has an approved consolidated... carrying out its strategic plan and its action plan. The performance report must include a description of...

24 CFR 91.520 - Performance reports.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Performance reports. 91.520 Section... Requirements § 91.520 Performance reports. (a) General. Each jurisdiction that has an approved consolidated... carrying out its strategic plan and its action plan. The performance report must include a description of...

24 CFR 91.520 - Performance reports.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Performance reports. 91.520 Section... Requirements § 91.520 Performance reports. (a) General. Each jurisdiction that has an approved consolidated... carrying out its strategic plan and its action plan. The performance report must include a description of...

24 CFR 91.520 - Performance reports.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Performance reports. 91.520 Section... Requirements § 91.520 Performance reports. (a) General. Each jurisdiction that has an approved consolidated... carrying out its strategic plan and its action plan. The performance report must include a description of...

24 CFR 91.520 - Performance reports.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Performance reports. 91.520 Section... Requirements § 91.520 Performance reports. (a) General. Each jurisdiction that has an approved consolidated... carrying out its strategic plan and its action plan. The performance report must include a description of...

46 CFR 91.25-30 - Electrical engineering equipment.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

46 CFR 91.25-30 - Electrical engineering equipment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

46 CFR 91.25-30 - Electrical engineering equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

46 CFR 91.25-30 - Electrical engineering equipment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

46 CFR 91.25-30 - Electrical engineering equipment.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Electrical engineering equipment. 91.25-30 Section 91.25... INSPECTION AND CERTIFICATION Inspection for Certification § 91.25-30 Electrical engineering equipment. For inspection procedures of electrical engineering equipment and systems see subchapter J (Electrical...

49 CFR 91.7 - Determination of compensatory charges.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 1 2010-10-01 2010-10-01 false Determination of compensatory charges. 91.7 Section 91.7 Transportation Office of the Secretary of Transportation INTERNATIONAL AIR TRANSPORTATION FAIR COMPETITIVE PRACTICES § 91.7 Determination of compensatory charges. (a) Upon indication by the...

The interaction of melanin with ionizing and UVC radiations: Characterization of thymine damage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huselton, C.A.

1988-01-01

These studies were undertaken to determine whether melanin could protect DNA against the harmful effects of ionizing or UVC radiations. A simple, in vitro, model system was developed to evaluate eumelanin (Sigma melanin) as a radioprotector of solutions of 0.1 mM thymine or thymidine exposed to 570Gy of ionizing radiation. Sigma melanin was compared to several amino acids, other biomolecules or to other forms of melanin. To investigate the role of melanin as a passive screen of UVC radiation, melanotic (I{sub 3}), amelanotic (AMEL) cells (both derived from a Cloudman S91 melanoma) and non-melanotic (EMT6) cells were labelled with radioactivemore » dTHd and exposed to 0, 1, 5 or 10KJ/m{sup 2} of UVC. The DNA was extracted; the bases hydrolyzed with concentrated HCl. Thymine bases were separated by reverse phase HPLC. No difference in dimer content was observed between I{sub 3} and AMEL cells, but EMT6 cells had nearly twice the amount of dimer. Overall thymine degradation was more pronounced in I{sub 3} cells than in the other two cell lines, due to the production of non-dimer thymine damage. This damage was identified as thymine glycol by HPLC and mass spectrometry. Melanin, upon exposure to UVC, appears to enhance thymine damage by producing oxidative damage.« less

50 CFR 91.3 - Public attendance at contest.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Public attendance at contest. 91.3 Section 91.3 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MISCELLANEOUS PROVISIONS MIGRATORY BIRD HUNTING AND CONSERVATION STAMP CONTEST Introduction § 91...

50 CFR 91.3 - Public attendance at contest.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false Public attendance at contest. 91.3 Section 91.3 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MISCELLANEOUS PROVISIONS MIGRATORY BIRD HUNTING AND CONSERVATION STAMP CONTEST Introduction § 91...

40 CFR 91.417 - Fuel flow measurement specifications.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Fuel flow measurement specifications. 91.417 Section 91.417 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... Procedures § 91.417 Fuel flow measurement specifications. (a) Fuel flow measurement is required only for raw...

7 CFR 958.91 - Order with marketing agreement.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 8 2011-01-01 2011-01-01 false Order with marketing agreement. 958.91 Section 958.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... § 958.91 Order with marketing agreement. Each signatory handler requests the Secretary to issue...

7 CFR 958.91 - Order with marketing agreement.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 8 2014-01-01 2014-01-01 false Order with marketing agreement. 958.91 Section 958.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... § 958.91 Order with marketing agreement. Each signatory handler requests the Secretary to issue...

7 CFR 958.91 - Order with marketing agreement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 8 2010-01-01 2010-01-01 false Order with marketing agreement. 958.91 Section 958.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... § 958.91 Order with marketing agreement. Each signatory handler requests the Secretary to issue...

7 CFR 958.91 - Order with marketing agreement.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 8 2013-01-01 2013-01-01 false Order with marketing agreement. 958.91 Section 958.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... § 958.91 Order with marketing agreement. Each signatory handler requests the Secretary to issue...

APOPTOSIS IN WHOLE MOUSE OVARIES

EPA Science Inventory

Apoptosis in Whole Mouse Ovaries
Robert M. Zucker Susan C. Jeffay and Sally D. Perreault
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina, 27711.

40 CFR 91.1009 - Export exemptions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Export exemptions. 91.1009 Section 91....1009 Export exemptions. (a) A new marine SI engine intended solely for export, and so labeled or tagged...., Washington, DC 20460. New marine SI engines exported to such countries must comply with EPA certification...

14 CFR 91.117 - Aircraft speed.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Aircraft speed. 91.117 Section 91.117... speed. (a) Unless otherwise authorized by the Administrator, no person may operate an aircraft below 10... than the maximum speed prescribed in this section, the aircraft may be operated at that minimum speed...

14 CFR 91.117 - Aircraft speed.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Aircraft speed. 91.117 Section 91.117... speed. (a) Unless otherwise authorized by the Administrator, no person may operate an aircraft below 10... than the maximum speed prescribed in this section, the aircraft may be operated at that minimum speed...

40 CFR 98.91 - Reporting threshold.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 22 2012-07-01 2012-07-01 false Reporting threshold. 98.91 Section 98...) MANDATORY GREENHOUSE GAS REPORTING Electronics Manufacturing § 98.91 Reporting threshold. (a) You must... year emission threshold in § 98.2(a)(2), follow the requirements of § 98.2(b), with one exception...

9 CFR 91.26 - Concrete flooring.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Concrete flooring. 91.26 Section 91.26 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS INSPECTION AND HANDLING OF LIVESTOCK FOR EXPORTATION Inspection of...