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Sample records for controls lipid flow

  1. Controlling water flow inside carbon nanotube with lipid membranes

    SciTech Connect

    Feng, Jia-Wei; Ding, Hong-Ming; Ma, Yu-Qiang

    2014-09-07

    Understanding and controlling the transportation of water molecules across carbon nanotube (CNT) is of great importance in bio-nanotechnology. In this paper, we systematically investigate the water transporting behaviors (i.e., water flow rate) inside the CNT in the presence of lipid membranes by using all atom molecular dynamic simulations. Our results show that the hydrophilicity of CNT as well as membrane thickness can have important impacts on the water flow rate. Interestingly, since the membrane thickness is temperature-dependent, the water flow rate can exhibit thermo-responsive behaviors. Further, we also provide insights into the effect of CNT on lipid membranes. It is found that all CNTs can increase the lipid tail order parameters and thicken the membrane at 320 K; while these effects are not obvious at 290 K. Importantly, we observe that the CNT with specific hydrophobicity has the least effect on membranes. The present study may give some useful advice on future experimental design of novel devices and sensors.

  2. Analysis of lipid flow on minimal surfaces

    NASA Astrophysics Data System (ADS)

    Bahmani, Fatemeh; Christenson, Joel; Rangamani, Padmini

    2016-03-01

    Interaction between the bilayer shape and surface flow is important for capturing the flow of lipids in many biological membranes. Recent microscopy evidence has shown that minimal surfaces (planes, catenoids, and helicoids) occur often in cellular membranes. In this study, we explore lipid flow in these geometries using a `stream function' formulation for viscoelastic lipid bilayers. Using this formulation, we derive two-dimensional lipid flow equations for the commonly occurring minimal surfaces in lipid bilayers. We show that for three minimal surfaces (planes, catenoids, and helicoids), the surface flow equations satisfy Stokes flow equations. In helicoids and catenoids, we show that the tangential velocity field is a Killing vector field. Thus, our analysis provides fundamental insight into the flow patterns of lipids on intracellular organelle membranes that are characterized by fixed shapes reminiscent of minimal surfaces.

  3. Lipid phase control of DNA delivery

    SciTech Connect

    Koynova, Rumiana; Wang, Li; Tarahovsky, Yury; MacDonald, Robert C.

    2010-01-18

    Cationic lipids form nanoscale complexes (lipoplexes) with polyanionic DNA and can be utilized to deliver DNA to cells for transfection. Here we report the correlation between delivery efficiency of these DNA carriers and the mesomorphic phases they form when interacting with anionic membrane lipids. Specifically, formulations that are particularly effective DNA carriers form phases of highest negative interfacial curvature when mixed with anionic lipids, whereas less effective formulations form phases of lower curvature. Structural evolution of the carrier lipid/DNA complexes upon interaction with cellular lipids is hence suggested as a controlling factor in lipid-mediated DNA delivery. A strategy for optimizing lipofection is deduced. The behavior of a highly effective lipoplex formulation, DOTAP/DOPE, is found to conform to this 'efficiency formula'.

  4. Intelligent Flow Control Valve

    NASA Technical Reports Server (NTRS)

    Kelley, Anthony R (Inventor)

    2015-01-01

    The present invention is an intelligent flow control valve which may be inserted into the flow coming out of a pipe and activated to provide a method to stop, measure, and meter flow coming from the open or possibly broken pipe. The intelligent flow control valve may be used to stop the flow while repairs are made. Once repairs have been made, the valve may be removed or used as a control valve to meter the amount of flow from inside the pipe. With the addition of instrumentation, the valve may also be used as a variable area flow meter and flow controller programmed based upon flowing conditions. With robotic additions, the valve may be configured to crawl into a desired pipe location, anchor itself, and activate flow control or metering remotely.

  5. Gas flow control valve

    SciTech Connect

    Phlipot, J.R.; Pinkston, S.R.; Nurre, H.

    1988-02-09

    A compact gas flow control valve is described comprising a valve body having a first, rotor cavity-defining portion and a second cover portion covering the rotor cavity, at least one of the body portions including inlet means communicating with the rotor chamber for receiving gas under pressure for providing the gas to the rotor chamber, at least one of the body portions including outlet means for delivery of the gas by the flow control valve, a rotor within the rotor cavity, the rotor including a flat surface, a flow control plate carried by the rotor, the flow control plate covering and lying against the flat surface of the rotor, the rotor having ports opening through the rotor surface, the ports being of sufficiently large size as not to limit the flow of the gas therethrough. The flow control plate comprises a thin, flat metal disc provided with gas flow control orifices extending therethrough and spaced circumferentially around the disc and in registry with respective ones of the ports, the rotor being of substantially greater thickness than the disc, the gas flow control being of different sizes and passage means for providing communication between the outlet means and at least a selected one of the flow control plate origices, selector means for orienting the rotor to permit flow only through selected flow control plate orifices and a corresponding rotor port for delivery by the outlet means.

  6. Solid lipid budesonide microparticles for controlled release inhalation therapy.

    PubMed

    Mezzena, Matteo; Scalia, Santo; Young, Paul M; Traini, Daniela

    2009-12-01

    A solid lipid microparticle system containing budesonide was prepared by oil in water emulsification followed by spray drying. The solid lipid system was studied in terms of morphology, particle size distribution, crystallinity, thermal properties, aerosol performance, and dissolution/diffusion release. The microparticle system was also compared to conventional spray-dried crystalline and amorphous budesonide samples. The particle size distributions of the crystalline, amorphous, and solid lipid microparticles, measured by laser diffraction, were similar; however, the microparticle morphology was more irregular than the spray-dried drug samples. The thermal response of the solid lipid microparticles suggested polymorphic transition and melting of the lipid, glycerol behenate (at approximately 48 degrees C and approximately 72 degrees C). No budesonide melting or crystallisation peaks were observed, suggesting that the budesonide was integrated into the matrix. X-ray powder diffraction patterns of the crystalline and amorphous budesonide were consistent with previous studies while the solid lipid microparticles showed two peaks, at approximately 21.3 and 23.5 2theta suggesting the metastable sub-alpha and primarily beta' form. Analysis of the in vitro diffusion/dissolution of the formulations was studied using a flow through model and curves analysed using difference/similarity factors and fitted using the Higuchi model. Regression analysis of this data set indicated differences in the t (0.5), where values of 49.7, 35.3, and 136.9 min were observed for crystalline, amorphous, and the solid lipid microparticles, respectively. The aerosol performance (<5 microm), measured by multistage liquid impinger, was 29.5%, 27.3%, and 21.1 +/- 0.6% for the crystalline, amorphous, and the solid lipid microparticles, respectively. This study has shown that solid lipid microparticles may provide a useful approach to controlled release respiratory therapy. PMID:19908147

  7. Ultrastructural and flow cytometric analyses of lipid accumulation in microalgae

    SciTech Connect

    Solomon, J.A.; Hand, R.E. Jr.; Mann, R.C.

    1986-12-01

    Lipid accumulation in three species of microalgae was investigated with flow cytometry (FCM) and transmission electron microscopy (TEM). Previous studies using batch cultures of a algae have led to the assumption that lipid accumulation in microalgae is a gradual process requiring at least several days for completion. However, FCM reveals, through changes in the chlorophyll:lipid ratio, that the time span required for individual cells to change metabolic state is short. Simultaneous FCM measurements of chlorophyll and nile red (neutral lipid) fluorescence in individual cells of nitrogen-deficient Isochrysis populations revealed a bimodal population distribution as one stage in the lipid accumulation process. The fact that two discrete populations exist, with few cells in an intermediate stage, suggests rapid response to a liqid trigger. Interpretations of light and electron microscopic observations are consistent with this hypothesis. The time required for an entire population to achieve maximum lipid content is considerably longer than that required for a single cell, due to the variation in response time among cells. In this study high lipid cultures were sometimes obtained by using FCM to separate high lipid cells from the remainder of the population. FCM holds much promise for strain enhancement but considerable developmental work, directed at providing more consistent results, remains to be done. 8 refs., 35 figs.

  8. Selective breakup of lipid vesicles under acoustic microstreaming flow

    NASA Astrophysics Data System (ADS)

    Pommella, Angelo; Garbin, Valeria

    2014-11-01

    The dynamics of lipid vesicles under small deformation in simple shear flow is well characterized: complex behaviors such as tumbling, breathing, and tank-treading are observed depending on the viscosity contrast between inner and outer fluid, vesicle excess area, membrane viscosity, and bending modulus. In contrast, phenomena upon large deformation are still poorly understood, in particular vesicle breakup. Simple shear flow geometries do not allow to reach the large stresses necessary to cause vesicle breakup. We use the acoustic microstreaming flow generated by an oscillating microbubble to study the large deformation and breakup of giant unilamellar vesicles. The deformation is governed by a capillary number based on the membrane elasticity K : Ca = ηγ˙a / K where η is the viscosity of the outer fluid, a the vesicle radius, and γ˙ the shear rate. We explore the effect of the mechanical properties of the membrane, and demonstrated selective breakup of vesicles based on the difference in membrane elasticity. The results reveal the influence of membrane mechanical properties in shear-induced vesicle breakup and the possibility to control in a quantitative way the selectivity of the process, with potential applications in biomedical technologies. The authors acknowledge funding from EU/FP7 Grant Number 618333.

  9. Microelectromechanical flow control apparatus

    DOEpatents

    Okandan, Murat

    2009-06-02

    A microelectromechanical (MEM) flow control apparatus is disclosed which includes a fluid channel formed on a substrate from a first layer of a nonconducting material (e.g. silicon nitride). A first electrode is provided on the first layer of the nonconducting material outside the flow channel; and a second electrode is located on a second layer of the nonconducting material above the first layer. A voltage applied between the first and second electrodes deforms the fluid channel to increase its cross-sectional size and thereby increase a flow of a fluid through the channel. In certain embodiments of the present invention, the fluid flow can be decreased or stopped by applying a voltage between the first electrode and the substrate. A peristaltic pumping of the fluid through the channel is also possible when the voltage is applied in turn between a plurality of first electrodes and the substrate. A MEM flow control assembly can also be formed by providing one or more MEM flow control devices on a common substrate together with a submicron filter. The MEM flow control assembly can optionally include a plurality of pressure sensors for monitoring fluid pressure and determining flow rates through the assembly.

  10. Control of the morphology of lipid layers by substrate surface chemistry.

    PubMed

    Granqvist, Niko; Yliperttula, Marjo; Välimäki, Salla; Pulkkinen, Petri; Tenhu, Heikki; Viitala, Tapani

    2014-03-18

    In this study, surface coatings were used to control the morphology of the deposited lipid layers during vesicle spreading, i.e., to control if liposomes self-assemble on a surface into a supported lipid bilayer or a supported vesicular layer. The influence of the properties of the surface coating on formation of the deposited lipid layer was studied with quartz crystal microbalance and two-wavelength multiparametric surface plasmon resonance techniques. Control of lipid self-assembly on the surface was achieved by two different types of soft substrate materials, i.e., dextran and thiolated polyethylene glycol, functionalized with hydrophobic linkers for capturing the lipid layer. The low-molecular-weight dextran-based surface promoted formation of supported lipid bilayers, while the thiolated polyethylene glycol-based surface promoted supported vesicular layer formation. A silicon dioxide surface was used as a reference surface in both measurement techniques. In addition to promoting supported lipid bilayer formation of known lipid mixtures, the dextran surface also promoted supported lipid bilayer formation of vesicles containing the cell membrane extract of human hepatoblastoma cells. The new dextran-based surface was also capable of protecting the supported lipid bilayer against dehydration when exposed to a constant flow of air. The well-established quartz crystal microbalance technique was effective in determining the morphology of the formed lipid layer, while the two-wavelength surface plasmon resonance analysis enabled further complementary characterization of the adsorbed supported lipid bilayers and supported vesicular layers. PMID:24564782

  11. Tank depletion flow controller

    DOEpatents

    Georgeson, Melvin A.

    1976-10-26

    A flow control system includes two bubbler tubes installed at different levels within a tank containing such as radioactive liquid. As the tank is depleted, a differential pressure transmitter monitors pressure differences imparted by the two bubbler tubes at a remote, shielded location during uniform time intervals. At the end of each uniform interval, balance pots containing a dense liquid are valved together to equalize the pressures. The resulting sawtooth-shaped signal generated by the differential pressure transmitter is compared with a second sawtooth signal representing the desired flow rate during each time interval. Variations in the two signals are employed by a control instrument to regulate flow rate.

  12. Optimal Flow Control Design

    NASA Technical Reports Server (NTRS)

    Allan, Brian; Owens, Lewis

    2010-01-01

    In support of the Blended-Wing-Body aircraft concept, a new flow control hybrid vane/jet design has been developed for use in a boundary-layer-ingesting (BLI) offset inlet in transonic flows. This inlet flow control is designed to minimize the engine fan-face distortion levels and the first five Fourier harmonic half amplitudes while maximizing the inlet pressure recovery. This concept represents a potentially enabling technology for quieter and more environmentally friendly transport aircraft. An optimum vane design was found by minimizing the engine fan-face distortion, DC60, and the first five Fourier harmonic half amplitudes, while maximizing the total pressure recovery. The optimal vane design was then used in a BLI inlet wind tunnel experiment at NASA Langley's 0.3-meter transonic cryogenic tunnel. The experimental results demonstrated an 80-percent decrease in DPCPavg, the reduction in the circumferential distortion levels, at an inlet mass flow rate corresponding to the middle of the operational range at the cruise condition. Even though the vanes were designed at a single inlet mass flow rate, they performed very well over the entire inlet mass flow range tested in the wind tunnel experiment with the addition of a small amount of jet flow control. While the circumferential distortion was decreased, the radial distortion on the outer rings at the aerodynamic interface plane (AIP) increased. This was a result of the large boundary layer being distributed from the bottom of the AIP in the baseline case to the outer edges of the AIP when using the vortex generator (VG) vane flow control. Experimental results, as already mentioned, showed an 80-percent reduction of DPCPavg, the circumferential distortion level at the engine fan-face. The hybrid approach leverages strengths of vane and jet flow control devices, increasing inlet performance over a broader operational range with significant reduction in mass flow requirements. Minimal distortion level requirements

  13. Aircraft Laminar Flow Control

    NASA Technical Reports Server (NTRS)

    Joslin, Ronald D.

    1998-01-01

    Aircraft laminar flow control (LFC) from the 1930's through the 1990's is reviewed and the current status of the technology is assessed. Examples are provided to demonstrate the benefits of LFC for subsonic and supersonic aircraft. Early studies related to the laminar boundary-layer flow physics, manufacturing tolerances for laminar flow, and insect-contamination avoidance are discussed. LFC concept studies in wind-tunnel and flight experiments are the major focus of the paper. LFC design tools are briefly outlined for completeness.

  14. Fluoresceinated phosphoethanolamine for flow-cytometric measurement of lipid peroxidation.

    PubMed

    Maulik, G; Kassis, A I; Savvides, P; Makrigiorgos, G M

    1998-10-01

    A new lipophilic fluorescein probe (fluor-DHPE) has been identified that can assay lipid peroxidation in mammalian cells on a cell-by-cell or selected-cell-subpopulation basis by flow cytometry. Application of this approach requires that the fluorescent probe be nonexchangeable among cells. Fluorescein is an appropriate fluorophore, since its fluorescence matches the specifications of common flow cytometers and the compound loses its fluorescence upon reaction with peroxyl radicals. Upon examination of four lipophilic derivatives of fluorescein, fluor-DHPE was found to be the only probe that was nonexchangeable among labeled and unlabeled rat RBC for at least 24 h. The exposure of fluor-DHPE-labeled RBC to benzoyl peroxide followed by mixing the sample with RBC unexposed to peroxide led to a decrease in fluorescence. Furthermore, the flow cytometer could clearly select the subpopulation of cells undergoing lipid peroxidation from those cells that were not. Fluor-DHPE-labeled-RBC obtained from rats and exposed to cumene hydroperoxide also displayed a gradual decrease in fluorescence. This decrease was preventable by either regulation of the vitamin E content in the animal diet or in vitro supplementation of cells with vitamin E. We conclude that fluor-DHPE is a stable and nonexchangeable probe for monitoring lipid peroxidation in cell subpopulations by flow cytometry. PMID:9801063

  15. Flow control using ferrofluids

    NASA Astrophysics Data System (ADS)

    Cornat, Francois; Beck, David; Jacobi, Ian; Stone, Howard

    2013-11-01

    A novel flow control technique is proposed which employs a ferrofluidic lubricant infused in a micro-patterned substrate as a ``morphing surface'' for control of wall-bounded flows. Traditionally, morphing surfaces produce dynamic changes in the curvature and roughness of solid substrates for active control of high Reynolds number flow features such as boundary layer separation and turbulent streaks. We show how these surface modifications can be achieved with a thin liquid layer in the presence of a normal magnetic field. By impregnating a chemically-treated, micro-patterned surface with a fluorinated ferrofluid, the fluid is maintained as a thin super-hydrophobic film and can be redistributed on the substrate by magnetic forces to dynamically reveal or conceal the underlying surface roughness. Moreover, the surface topography of the ferrofluid film itself can be modified to produce an enhanced roughness, beyond the scale of the underlying substrate pattern. Both types of ferrofluidic surface modifications are studied in micro- and macro- scale channels in order to assess the feasibility of flow modification at low to moderate Reynolds numbers.

  16. Fluidic flow control

    SciTech Connect

    Tippetts, J.R.

    1987-01-01

    Liquid and gaseous product streams are the lifeblood of many industries. Safe, reliable fluid handling is of the utmost importance. Here, no-moving-part fluidic systems have unique advantages which are now clear in such diverse fields as flood control, nuclear plant and ventilation. This book stems from these applications which typically use vortex diodes, amplifiers, jet-pump-like elements and special junctions to control aggressive fluid flows. Both fluid-mechanics and network theory are combined to give the theoretical background.

  17. Vortex generator for flow control

    NASA Technical Reports Server (NTRS)

    Collins, Jr., Earl R. (Inventor); Marner, Wilbur J. (Inventor); Rohatgi, Naresh K. (Inventor)

    1989-01-01

    Fluidics flow control of a multiphase supply using a cylindrical chamber is achieved by introducing the supply flow radially into the chamber. The supply flow exits through a port in the center at the chamber. A control fluid is then introduced tangentially about 90.degree. upstream from the supply port. A second control fluid port may be added about 90.degree. upstream from the first control fluid port, but preferably two sets of supply and control ports are added with like ports diametrically opposite each other. The control fluid flows against the circular wall of the control chamber, which introduces a vortex in the flow of the supply flow that decays into a spiral path to the exit port in the center of the chamber. The control flow rate may thus be used to control the spiral path, and therefore the supply flow rate through the exit port.

  18. Reversible control of current across lipid membranes by local heating

    NASA Astrophysics Data System (ADS)

    Urban, Patrick; Kirchner, Silke R.; Mühlbauer, Christian; Lohmüller, Theobald; Feldmann, Jochen

    2016-03-01

    Lipid membranes are almost impermeable for charged molecules and ions that can pass the membrane barrier only with the help of specialized transport proteins. Here, we report how temperature manipulation at the nanoscale can be employed to reversibly control the electrical resistance and the amount of current that flows through a bilayer membrane with pA resolution. For this experiment, heating is achieved by irradiating gold nanoparticles that are attached to the bilayer membrane with laser light at their plasmon resonance frequency. We found that controlling the temperature on the nanoscale renders it possible to reproducibly regulate the current across a phospholipid membrane and the membrane of living cells in absence of any ion channels.

  19. Reversible control of current across lipid membranes by local heating

    PubMed Central

    Urban, Patrick; Kirchner, Silke R.; Mühlbauer, Christian; Lohmüller, Theobald; Feldmann, Jochen

    2016-01-01

    Lipid membranes are almost impermeable for charged molecules and ions that can pass the membrane barrier only with the help of specialized transport proteins. Here, we report how temperature manipulation at the nanoscale can be employed to reversibly control the electrical resistance and the amount of current that flows through a bilayer membrane with pA resolution. For this experiment, heating is achieved by irradiating gold nanoparticles that are attached to the bilayer membrane with laser light at their plasmon resonance frequency. We found that controlling the temperature on the nanoscale renders it possible to reproducibly regulate the current across a phospholipid membrane and the membrane of living cells in absence of any ion channels. PMID:26940847

  20. Flow Control Effectiveness at High Speed Flows

    NASA Astrophysics Data System (ADS)

    Kontis, K.; Lada, C.

    2005-02-01

    The effects of two important flow control techniques, i.e. jet control and dimples, on the aerodynamic characteristics and performance of a number of body configurations have been studied experimentally. The dimple studies have been carried out in a transonic-supersonic wind tunnel and the jet studies in a hypersonic gun tunnel at a Mach number of 8.2. Air was used as the working gas. The tests employed schlieren photography and oil-flow to study the overall flow field. Quantitative studies have been made by pressure measurements.

  1. Flow-control restrictor

    SciTech Connect

    Bradley, A.H.; Knowles, S.M.; Pilon, F.J.

    1990-01-30

    This patent describes a flow control restrictor characterized by its low operational noise level. It comprises: an elongated body having a longitudinal axis, a first end surface, a nose region adjacent the first end surface, a peripheral spider region having a radius, a second end surface and an axial bore intersecting the first and second end surfaces. The first end surface being substantially planar and substantially perpendicular to the axis. The nose region including a cylindrical nose surface having a radius and a convex transition surface constituting the intersection of the first end surface and the nose surface having a radius. The transition surface comprising a segment of a sphere having its center upon the longitudinal axis and a radius greater than a radius of the nose surface.

  2. Flow Instability and Flow Control Scaling Laws

    NASA Astrophysics Data System (ADS)

    van Ness, Daniel; Corke, Thomas; Morris, Scott

    2006-11-01

    A flow instability that is receptive to perturbations is present in the tip clearance leakage flow over the tip of a turbine blade. This instability was investigated through the introduction of active flow control in the viscous flow field. Control was implemented in the form of a dielectric barrier discharge created by a weakly-ionized plasma actuation arrangement. The experimental setup consisted of a low-speed linear turbine cascade made up of an array of nine Pratt & Whitney ``PakB'' turbine blades. This idealized cascade configuration was used to examine the tip clearance leakage flow that exists within the low pressure turbine stage of a gas-turbine engine. The center blade of the cascade array had a variable tip clearance up to five percent chord. Reynolds numbers based on axial blade chord varied from 10^4 to 10^5. Multi-port pressure probe measurements, as well as Stereo Particle Image Velocimetry were used to document the dependence of the instability on the frequency and amplitude of flow control perturbations. Scaling laws based on the variation of blade tip clearance height and inflow conditions were investigated. These results permitted an improved understanding of the mechanism of flow instability.

  3. Supersonic Laminar Flow Control Research

    NASA Technical Reports Server (NTRS)

    Lo, Ching F.; Wiberg, Clark G.

    1996-01-01

    The objective of this research is to understand supersonic laminar flow stability, transition and active control. Some prediction techniques will be developed or modified to analyze laminar flow stability. The effects of distributed heating and cooling as an active boundary layer control technique will be studied. The primary tasks of the research apply to the NASA/Ames PoC and LFSWT's nozzle design with laminar flow control and are listed as follows: Predictions of supersonic laminar boundary layer stability and transition; Effects of wall heating and cooling on supersonic laminar flow control on a flat plate; Performance evaluation of the PoC and LFSWT nozzle designs with wall heating and cooling applied at different locations and various lengths; Effects of a conducted-vs-pulse wall temperature distribution for the LFSWT; and Application of wall heating and/or cooling to laminar boundary layer and flow separation control of airfoils and investigation of related active control techniques.

  4. A Lipid Gate for the Peripheral Control of Pain

    PubMed Central

    Hohmann, Andrea G.; Seybold, Virginia; Hammock, Bruce D.

    2014-01-01

    Cells in injured and inflamed tissues produce a number of proalgesic lipid-derived mediators, which excite nociceptive neurons by activating selective G-protein-coupled receptors or ligand-gated ion channels. Recent work has shown that these proalgesic factors are counteracted by a distinct group of lipid molecules that lower nociceptor excitability and attenuate nociception in peripheral tissues. Analgesic lipid mediators include endogenous agonists of cannabinoid receptors (endocannabinoids), lipid-amide agonists of peroxisome proliferator-activated receptor-α, and products of oxidative metabolism of polyunsaturated fatty acids via cytochrome P450 and other enzyme pathways. Evidence indicates that these lipid messengers are produced and act at different stages of inflammation and the response to tissue injury, and may be part of a peripheral gating mechanism that regulates the access of nociceptive information to the spinal cord and the brain. Growing knowledge about this peripheral control system may be used to discover safer medicines for pain. PMID:25392487

  5. Lipid partitioning at the nuclear envelope controls membrane biogenesis

    PubMed Central

    Barbosa, Antonio Daniel; Sembongi, Hiroshi; Su, Wen-Min; Abreu, Susana; Reggiori, Fulvio; Carman, George M.; Siniossoglou, Symeon

    2015-01-01

    Partitioning of lipid precursors between membranes and storage is crucial for cell growth, and its disruption underlies pathologies such as cancer, obesity, and type 2 diabetes. However, the mechanisms and signals that regulate this process are largely unknown. In yeast, lipid precursors are mainly used for phospholipid synthesis in nutrient-rich conditions in order to sustain rapid proliferation but are redirected to triacylglycerol (TAG) stored in lipid droplets during starvation. Here we investigate how cells reprogram lipid metabolism in the endoplasmic reticulum. We show that the conserved phosphatidate (PA) phosphatase Pah1, which generates diacylglycerol from PA, targets a nuclear membrane subdomain that is in contact with growing lipid droplets and mediates TAG synthesis. We find that cytosol acidification activates the master regulator of Pah1, the Nem1-Spo7 complex, thus linking Pah1 activity to cellular metabolic status. In the absence of TAG storage capacity, Pah1 still binds the nuclear membrane, but lipid precursors are redirected toward phospholipids, resulting in nuclear deformation and a proliferation of endoplasmic reticulum membrane. We propose that, in response to growth signals, activation of Pah1 at the nuclear envelope acts as a switch to control the balance between membrane biogenesis and lipid storage. PMID:26269581

  6. Supersonic Laminar Flow Control Research

    NASA Technical Reports Server (NTRS)

    Lo, C. F.; Wiberg, Clark G.

    1996-01-01

    The objective of this research is to understand supersonic laminar flow stability, transition and active control. Some prediction techniques are developed or modified to analyze laminar flow stability. The effects of distributed heating and cooling as an active boundary layer control technique are studied. The primary tasks of the research apply to the NASA/Ames Proof-of-Concept (PoC) and the Laminar Flow Supersonic Wind Tunnel's (LFSWT's) nozzle design with laminar flow control and are listed as follows: (1) Predictions of supersonic laminar boundary layer stability and transition; (2) Effects of wall heating and cooling on supersonic laminar flow control on a flat plate; (3) Performance evaluation of the PoC and LFSWT nozzle designs with wall heating and cooling applied at different locations and various lengths; (4) Effects of a conducted -vs- pulse wall temperature distribution for the LFSWT; and (5) Application of wall heating and/or cooling to laminar boundary layer and flow separation control of airfoils and investigation of related active control techniques.

  7. Control of lipid oxidation at the mitochondrial level.

    PubMed

    Sahlin, Kent

    2009-06-01

    The rate of lipid oxidation during exercise is controlled at several sites, and there is a reciprocal dependency between oxidation of lipids and carbohydrates (CHO). It is well known that the proportion of the 2 fuels oxidized is influenced by substrate availability and exercise intensity, but the mechanisms regulating fuel preferences remain unclear. During intense exercise, oxidation of long-chain fatty acids (LCFAs) decreases, and the major control is likely to be at the mitochondrial level. Potential mitochondrial sites for control of lipid oxidation include transport of LCFAs into mitochondrial matrix, beta-oxidation, the tricarboxylic acid cycle, and the electron transport chain (ETC). CHO catabolism may impair lipid oxidation by interfering with the transfer of LCFAs into mitochondria and by competing for mutual cofactors (i.e., nicotinamide adenine dinucleotide and (or) coenzyme A (CoA)). The different effect of energy state on the catabolism of CHO and lipids is likely to be of major importance in explaining the shift in fuel utilization during intensive exercise. Formation of acetyl-CoA from CHO is activated by a low energy state, and will lead to accumulation of products that are inhibitory to lipid oxidation. In contrast, beta-oxidation of LCFAs to acetyl-CoA is not stimulated by a low energy state. Further interaction between CHO and LCFAs may occur by substrate competition for electron carriers at ETC, due to provisions of electrons through different complexes. Feedback inhibition of beta-oxidation by redox state is thought to be an important mechanism for the slowing of lipid oxidation during intensive exercise. PMID:19448703

  8. Supersonic laminar flow control

    NASA Technical Reports Server (NTRS)

    Bushnell, D. M.

    1992-01-01

    A development status evaluation is presented for the theoretical understanding and design conceptualization of boundary layer control (BLC) systems applicable to supersonic transports, such as the currently envisioned NASA High Speed Civil Transport. By reducing fuel burned, supersonic BLC techniques could expand ranges to Pacific-crossing scales, while lowering sonic boom effects and upper-atmosphere pollution and even reducing skin friction temperature. The critical consideration for supersonic BLC is the presence of wave effects.

  9. Biomimetic Flow Control

    NASA Technical Reports Server (NTRS)

    Anders, John B.

    2000-01-01

    Biologic flight has undoubtedly intrigued man for thousands of years, yet it has been only the last 100 years or so that any serious challenge has been mounted to the pre-eminence of birds. Although present-day large-scale aircraft are now clearly able to fly higher, faster and farther than any bird or insect, it is obvious that these biological creatures have a mastery of low Reynolds number, unsteady flows that is unrivaled by man-made systems. This paper suggests that biological flight should be examined for mechanisms that may apply to engineered flight systems, especially in the emerging field of small-scale, uninhabited aerial vehicles (UAV). This paper discusses the kinematics and aerodynamics of bird and insect flight, including some aspects of unsteady aerodynamics. The dynamics of flapping wing flight is briefly examined, including gait selection, flapping frequency and amplitude selection, as well as wing planform and angle-of-attack dynamics. Unsteady aerodynamic mechanisms as practiced by small birds and insects are reviewed. Drag reduction morphologies of birds and marine animals are discussed and fruitful areas of research are suggested.

  10. Pressure compensated flow control valve

    DOEpatents

    Minteer, Daniel J.

    1999-01-01

    The invention is an air flow control valve which is capable of maintaining a constant flow at the outlet despite changes in the inlet or outlet pressure. The device consists of a shell assembly with an inlet chamber and outlet chamber separated by a separation plate. The chambers are connected by an orifice. Also located within the inlet chamber is a port controller assembly. The port controller assembly consists of a differential pressure plate and port cap affixed thereon. The cap is able to slide in and out of the orifice separating the inlet and outlet chambers. When the pressure differential is sufficient, the differential pressure plate rises or falls to maintain a constant air flow. Movement of the port controller assembly does not require the use of seals, diaphragms, tight tolerances, bushings, bearings, hinges, guides, or lubricants.

  11. The central melanocortin system directly controls peripheral lipid metabolism

    PubMed Central

    Nogueiras, Ruben; Wiedmer, Petra; Perez-Tilve, Diego; Veyrat-Durebex, Christelle; Keogh, Julia M.; Sutton, Gregory M.; Pfluger, Paul T.; Castaneda, Tamara R.; Neschen, Susanne; Hofmann, Susanna M.; Howles, Philip N.; Morgan, Donald A.; Benoit, Stephen C.; Szanto, Ildiko; Schrott, Brigitte; Schürmann, Annette; Joost, Hans-Georg; Hammond, Craig; Hui, David Y.; Woods, Stephen C.; Rahmouni, Kamal; Butler, Andrew A.; Farooqi, I. Sadaf; O’Rahilly, Stephen; Rohner-Jeanrenaud, Françoise; Tschöp, Matthias H.

    2007-01-01

    Disruptions of the melanocortin signaling system have been linked to obesity. We investigated a possible role of the central nervous melanocortin system (CNS-Mcr) in the control of adiposity through effects on nutrient partitioning and cellular lipid metabolism independent of nutrient intake. We report that pharmacological inhibition of melanocortin receptors (Mcr) in rats and genetic disruption of Mc4r in mice directly and potently promoted lipid uptake, triglyceride synthesis, and fat accumulation in white adipose tissue (WAT), while increased CNS-Mcr signaling triggered lipid mobilization. These effects were independent of food intake and preceded changes in adiposity. In addition, decreased CNS-Mcr signaling promoted increased insulin sensitivity and glucose uptake in WAT while decreasing glucose utilization in muscle and brown adipose tissue. Such CNS control of peripheral nutrient partitioning depended on sympathetic nervous system function and was enhanced by synergistic effects on liver triglyceride synthesis. Our findings offer an explanation for enhanced adiposity resulting from decreased melanocortin signaling, even in the absence of hyperphagia, and are consistent with feeding-independent changes in substrate utilization as reflected by respiratory quotient, which is increased with chronic Mcr blockade in rodents and in humans with loss-of-function mutations in MC4R. We also reveal molecular underpinnings for direct control of the CNS-Mcr over lipid metabolism. These results suggest ways to design more efficient pharmacological methods for controlling adiposity. PMID:17885689

  12. Phase separation of lipid microdomains controlled by polymerized lipid bilayer matrices.

    PubMed

    Okazaki, Takashi; Tatsu, Yoshiro; Morigaki, Kenichi

    2010-03-16

    We developed a micropatterned model biological membrane on a solid substrate that can induce phase separation of lipid microdomains in a designed geometry. Micropatterned lipid bilayers were generated by the photolithographic polymerization of a diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC). By changing the UV dose for the photopolymerization, we could modulate the coverage of the surface by the polymeric bilayer domains. After removing nonpolymerized DiynePC, natural phospholipid membranes were incorporated into the micropatterned polymeric bilayer matrix by a self-assembly process (vesicle fusion). As we incorporated a ternary lipid mixture of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), sphingomyelin (SM), and cholesterol (Chol) (1:1:1), liquid ordered domains (Lo: rich in SM and Chol) were accumulated in the polymer free regions, whereas liquid disordered domains (Ld: rich in DOPC) preferentially participated into the partially polymeric bilayer regions. It was postulated that Ld domains preferentially came in contact with the polymeric bilayer boundaries because of their lower elastic moduli and a smaller thickness mismatch at the boundary. The effect of polymeric bilayer matrix to hinder the size growth of Lo domains should also be playing an important role. The controlled phase separation should open new possibilities to locally concentrate membrane proteins and other nanometer-sized materials on the substrate by associating them with the lipid microdomains. PMID:20020734

  13. Overview of Laminar Flow Control

    NASA Technical Reports Server (NTRS)

    Joslin, Ronald D.

    1998-01-01

    The history of Laminar Flow Control (LFC) from the 1930s through the 1990s is reviewed and the current status of the technology is assessed. Early studies related to the natural laminar boundary-layer flow physics, manufacturing tolerances for laminar flow, and insect-contamination avoidance are discussed. Although most of this publication is about slot-, porous-, and perforated-suction LFC concept studies in wind tunnel and flight experiments, some mention is made of thermal LFC. Theoretical and computational tools to describe the LFC aerodynamics are included for completeness.

  14. Hybrid laminar flow control study

    NASA Technical Reports Server (NTRS)

    1982-01-01

    Hybrid laminar flow control (HLFC) in which leading edge suction is used in conjunction with wing pressure distribution tailoring to postpone boundary layer transition and reduce friction drag was examined. Airfoil design characteristics required for laminar flow control (LFC) were determined. The aerodynamic design of the HLFC wing for a 178 passenger commercial turbofan transport was developed, and a drag was estimated. Systems changes required to install HLFC were defined, and weights and fuel economy were estimated. The potential for 9% fuel reduction for a 3926-km (2120-nmi) mission is identified.

  15. Supersonic laminar-flow control

    NASA Technical Reports Server (NTRS)

    Bushnell, Dennis M.; Malik, Mujeeb R.

    1987-01-01

    Detailed, up to date systems studies of the application of laminar flow control (LFC) to various supersonic missions and/or vehicles, both civilian and military, are not yet available. However, various first order looks at the benefits are summarized. The bottom line is that laminar flow control may allow development of a viable second generation SST. This follows from a combination of reduced fuel, structure, and insulation weight permitting operation at higher altitudes, thereby lowering sonic boom along with improving performance. The long stage lengths associated with the emerging economic importance of the Pacific Basin are creating a serious and renewed requirement for such a vehicle. Supersonic LFC techniques are discussed.

  16. Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows.

    PubMed

    Jönsson, Peter; Jönsson, Bengt

    2015-11-24

    It has previously been observed that an externally applied hydrodynamic shear flow above a fluid lipid bilayer can change the local concentration of macromolecules that are associated with the lipid bilayer. The external liquid flow results in a hydrodynamic force on molecules protruding from the lipid bilayer, causing them to move in the direction of the flow. However, there has been no quantitative study about the magnitude of these forces. We here use finite element simulations to investigate how the magnitude of the external hydrodynamic forces varies with the size and shape of the studied macromolecule. The simulations show that the hydrodynamic force is proportional to the effective hydrodynamic area of the studied molecule, Ahydro, multiplied by the mean hydrodynamic shear stress acting on the membrane surface, σhydro. The parameter Ahydro depends on the size and shape of the studied macromolecule above the lipid bilayer and scales with the cross-sectional area of the molecule. We also investigate how hydrodynamic shielding from other surrounding macromolecules decreases Ahydro when the surface coverage of the shielding macromolecules increases. Experiments where the protein streptavidin is anchored to a supported lipid bilayer on the floor of a microfluidic channel were finally performed at three different surface concentrations, Φ = 1%, 6%, and 10%, where the protein is being moved relative to the lipid bilayer by a liquid flow through the channel. From photobleaching measurements of fluorescently labeled streptavidin we found the experimental drift data to be within good accuracy of the simulated results, less than 12% difference, indicating the validity of the results obtained from the simulations. In addition to giving a deeper insight into how a liquid flow can affect membrane-associated molecules in a lipid bilayer, we also see an interesting potential of using hydrodynamic flow experiments together with the obtained results to study the size and

  17. Valve for controlling solids flow

    DOEpatents

    Staiger, M.D.

    1982-09-29

    A valve for controlling the flow of solids comprises a vessel having an overflow point, an inlet line for discharging solids into the vessel positioned within the vessel such that the inlet line's discharge point is lower than the vessel's overflow point, and means for introducing a fluidizing fluid into the vessel. The fluidizing fluid fluidizes the solids within the vessel so that they overflow at the vessel's overflow point. For the removal of nuclear waste product the vessel may be placed within a sealed container having a bottom connected transport line for transporting the solids to storage or other sites. The rate of solids flow is controlled by the flow rate of the fluidizing fluid and by V-notch weirs of different sizes spaced about the top of the vessel.

  18. Laminar flow control is maturing

    NASA Technical Reports Server (NTRS)

    Wagner, Richard D.; Bartlett, Dennis W.; Maddalon, Dal V.

    1988-01-01

    Recent research demonstrates that laminar flow (LF) can be reliable in flight and that the support system need not be complex. Shaping produces favorable pressure gradients for maintaining natural laminar flow (NLF), and laminar flow control (LFC) techniques such as full chord suction promise a fuel-saving payoff of up to 30 percent on long-range missions. For large aircraft, current research is concentrated on hybrid LFC concepts which combine suction and pressure-gradient control. At NASA Ames, an F-14 with variable wing sweep has been flight tested with smooth surface gloves on the wings; preliminary results indicate high transition Reynolds numbers to sweep angles as large as 25 deg. In addition, a 757 was flight tested with an NLF glove on the right wing just outboard of the engine pylon; and the LF was found to be suprisingly robust.

  19. Valve for controlling solids flow

    DOEpatents

    Staiger, M. Daniel

    1985-01-01

    A valve for controlling the flow of solids comprises a vessel having an overflow point, an inlet line for discharging solids into the vessel positioned within the vessel such that the inlet line's discharge point is lower than the vessel's overflow point, and apparatus for introducing a fluidizing fluid into the vessel. The fluidizing fluid fluidizes the solids within the vessel so that they overflow at the vessel's overflow point. For the removal of nuclear waste product the vessel may be placed within a sealed container having a bottom connected transport line for transporting the solids to storage or other sites. The rate of solids flow is controlled by the flow rate of the fluidizing fluid and by V-notch weirs of different sizes spaced about the top of the vessel.

  20. Physics of forebody flow control

    NASA Technical Reports Server (NTRS)

    Font, Gabriel I.

    1993-01-01

    Performance in the high angle of attack regime is required by many different types of aircraft. Military aircraft, such as fighters, utilize flight in this regime to improve maneuverability. Civilian aircraft, such as supersonic or hypersonic transports, will also need to operate in this regime during take off and landing, due to their small highly swept wings. Flight at high angles of attack is problematic due to the vortices being created on the nose of the aircraft. The vortices tend to become asymmetric and produce side forces. At the same time, the rudders are less effective because they are becoming immersed in the flow separating from the wings and fuselage. Consequently, the side force produced by the vortices on the nose tend to destabilize the aircraft. This situation may be corrected through the use of a forebody flow control system such as tangential slot blowing. In this concept, a jet is blown from the nose in an effort to alter the flow field around the nose and diminish the destabilizing side force. Alternately, the jet may be used to create a side force which could be used to augment the rudders. This would allow the size of the rudders to be decreased which would, in turn, diminish the cruise drag. Therefore, the use of a tangential slot blowing system has the potential for improving both the maneuver performance and the cruise performance of an aircraft. The present study was conducted to explore the physics of forebody flow control. The study consisted of two major thrusts: (1) exploration of forebody flow control with tangential slot blowing; (2) investigation of flow and field response to a general perturbation.

  1. Ferroelectric Fluid Flow Control Valve

    NASA Technical Reports Server (NTRS)

    Jalink, Antony, Jr. (Inventor); Hellbaum, Richard F. (Inventor); Rohrbach, Wayne W. (Inventor)

    1999-01-01

    An active valve is controlled and driven by external electrical actuation of a ferroelectric actuator to provide for improved passage of the fluid during certain time periods and to provide positive closure of the valve during other time periods. The valve provides improved passage in the direction of flow and positive closure in the direction against the flow. The actuator is a dome shaped internally prestressed ferroelectric actuator having a curvature, said dome shaped actuator having a rim and an apex. and a dome height measured from a plane through said rim said apex that varies with an electric voltage applied between an inside and an outside surface of said dome shaped actuator.

  2. Control of submersible vortex flows

    NASA Technical Reports Server (NTRS)

    Bushnell, D. M.; Donaldson, C. D.

    1990-01-01

    Vortex flows produced by submersibles typically unfavorably influence key figures of merit such as acoustic and nonacoustic stealth, control effectiveness/maneuverability, and propulsor efficiency/body drag. Sources of such organized, primarily longitudinal, vorticity include the basic body (nose and sides) and appendages (both base/intersection and tip regions) such as the fairwater, dive planes, rear control surfaces, and propulsor stators/tips. Two fundamentally different vortex control approaches are available: (1) deintensification of the amplitude and/or organization of the vortex during its initiation process; and (2) downstream vortex disablement. Vortex control techniques applicable to the initiation region (deintensification approach) include transverse pressure gradient minimization via altered body cross section, appendage dillets, fillets, and sweep, and various appendage tip and spanload treatment along with the use of active controls to minimize control surface size and motions. Vortex disablement can be accomplished either via use of control vortices (which can also be used to steer the vortices off-board), direct unwinding, inducement of vortex bursting, or segmentation/tailoring for enhanced dissipation. Submersible-applicable vortex control technology is also included derived from various aeronautical applications such as mitigation of the wing wake vortex hazard and flight aircraft maneuverability at high angle of attack as well as the status of vortex effects upon, and mitigation of, nonlinear control forces on submersibles. Specific suggestions for submersible-applicable vortex control techniques are presented.

  3. Multiverse data-flow control.

    PubMed

    Schindler, Benjamin; Waser, Jürgen; Ribičić, Hrvoje; Fuchs, Raphael; Peikert, Ronald

    2013-06-01

    In this paper, we present a data-flow system which supports comparative analysis of time-dependent data and interactive simulation steering. The system creates data on-the-fly to allow for the exploration of different parameters and the investigation of multiple scenarios. Existing data-flow architectures provide no generic approach to handle modules that perform complex temporal processing such as particle tracing or statistical analysis over time. Moreover, there is no solution to create and manage module data, which is associated with alternative scenarios. Our solution is based on generic data-flow algorithms to automate this process, enabling elaborate data-flow procedures, such as simulation, temporal integration or data aggregation over many time steps in many worlds. To hide the complexity from the user, we extend the World Lines interaction techniques to control the novel data-flow architecture. The concept of multiple, special-purpose cursors is introduced to let users intuitively navigate through time and alternative scenarios. Users specify only what they want to see, the decision which data are required is handled automatically. The concepts are explained by taking the example of the simulation and analysis of material transport in levee-breach scenarios. To strengthen the general applicability, we demonstrate the investigation of vortices in an offline-simulated dam-break data set. PMID:23559512

  4. Laminar flow control for transport aircraft applications

    NASA Technical Reports Server (NTRS)

    Wagner, R. D.

    1986-01-01

    The incorporation of laminar flow control into transport aircraft is discussed. Design concepts for the wing surface panel of laminar flow control transport aircraft are described. The development of small amounts of laminar flow on small commercial transports with natural or hybrid flow control is examined. Techniques for eliminating the insect contamination problem in the leading-edge region are proposed.

  5. Transcriptional control of adipose lipid handling by IRF4

    PubMed Central

    Eguchi, Jun; Wang, Xun; Yu, Songtao; Kershaw, Erin E.; Chiu, Patricia C.; Dushay, Joanne; Estall, Jennifer L.; Klein, Ulf; Maratos-Flier, Eleftheria; Rosen, Evan D.

    2011-01-01

    Summary Adipocytes store triglyceride during periods of nutritional affluence and release free fatty acids during fasting through coordinated cycles of lipogenesis and lipolysis. While much is known about the acute regulation of these processes during fasting and feeding, less is understood about the transcriptional basis by which adipocytes control lipid handling. Here we show that interferon regulatory factor 4 (IRF4) is a critical determinant of the transcriptional response to nutrient availability in adipocytes. Fasting induces IRF4 in an insulin- and FoxO1-dependent manner. IRF4 is required for lipolysis, at least in part due to direct effects on the expression of adipocyte triglyceride lipase and hormone-sensitive lipase. Conversely, reduction of IRF4 enhances lipid synthesis. Mice lacking adipocyte IRF4 exhibit increased adiposity and deficient lipolysis. These studies establish a link between IRF4 and the disposition of calories in adipose tissue, with consequences for systemic metabolic homeostasis. PMID:21356515

  6. Compressor Flow Control Concepts. 2; UEET Compressor Flow Control Modeling

    NASA Technical Reports Server (NTRS)

    Chima, Rodrick V.

    2001-01-01

    Several passive flow control devices have been modeled computationally in the Swift CFD code. The models were applied to the first stage rotor and stator of the baseline UEET compressor in an attempt to improve efficiency and/or stall margin. The devices included suction surface bleed, tip injection, self-aspirated rotors, area-ruled casing, and vortex generators. The models and computed results will be described in the presentation. None of the results have shown significant gains in efficiency; however, casing vortex generators have shown potential improvements in stall margin.

  7. Topological regulation of lipid balance in cells.

    PubMed

    Drin, Guillaume

    2014-01-01

    Lipids are unevenly distributed within and between cell membranes, thus defining organelle identity. Such distribution relies on local metabolic branches and mechanisms that move lipids. These processes are regulated by feedback mechanisms that decipher topographical information in organelle membranes and then regulate lipid levels or flows. In the endoplasmic reticulum, the major lipid source, transcriptional regulators and enzymes sense changes in membrane features to modulate lipid production. At the Golgi apparatus, lipid-synthesizing, lipid-flippase, and lipid-transport proteins (LTPs) collaborate to control lipid balance and distribution within the membrane to guarantee remodeling processes crucial for vesicular trafficking. Open questions exist regarding LTPs, which are thought to be lipid sensors that regulate lipid synthesis or carriers that transfer lipids between organelles across long distances or in contact sites. A novel model is that LTPs, by exchanging two different lipids, exploit one lipid gradient between two distinct membranes to build a second lipid gradient. PMID:24606148

  8. External and Turbomachinery Flow Control Working Group

    NASA Technical Reports Server (NTRS)

    Ahmadi, G.; Alstrom, B.; Colonius, T.; Dannenhoffer, J.; Glauser, M.; Helenbrook, B.; Higuchi, H.; Hodson, H.; Jha, R.; Kabiri, P.; LaGraff, J.; Low,K.; McKeon, B.; Morrison, J.; Obcid, S.; Orbaker, A.; Samimy, M.; Schmit, R.; Seifert, A.; Seume, J.; Shahabi, A.; Shea, P.; Ukeiley, L.; Wallace, R.

    2010-01-01

    Broad Flow Control Issues: a) Understanding flow physics. b) Specific control objective(s). c) Actuation. d) Sensors. e) Integrated active flow control system. f) Development of design tools (CFD, reduced order models, controller design, understanding and utilizing instabilities and other mechanisms, e.g., streamwise vorticity).

  9. Bio-mimetic Flow Control

    NASA Astrophysics Data System (ADS)

    Choi, Haecheon

    2009-11-01

    Bio-mimetic engineering or bio-mimetics is the application of biological methods and systems found in nature to the study and design of engineering systems and modern technology (from Wikipedia). The concept itself is old, but successful developments have been made recently, especially in the research field of flow control. The objective of flow control based on the bio-mimetic approach is to develop novel concepts for reducing drag, increasing lift and enhancing aerodynamic performance. For skin friction reduction, a few ideas have been suggested such as the riblet from shark, compliant surface from dolphin, microbubble injection and multiple front-body curvature from penguin, and V-shaped protrusion from sailfish. For form drag reduction, several new attempts have been also made recently. Examples include the V-shaped spanwise grooves from saguaro cactus, overall shape of box fish, longitudinal grooves on scallop shell, bill of swordfish, hooked comb on owl wing, trailing-edge protrusion on dragonfly wing, and fillet. For the enhancement of aerodynamic performance, focuses have been made on the birds, fish and insects: e.g., double layered feather of landing bird, leading-edge serration of humpback-whale flipper, pectoral fin of flying fish, long tail on swallowtail-butterfly wing, wing flapping motion of dragonfly, and alula in birds. Living animals adapt their bodies to better performance in multi purposes, but engineering requires single purpose in most cases. Therefore, bio-mimetic approaches often produce excellent results more than expected. However, they are sometimes based on people's wrong understanding of nature and produce unwanted results. Successes and failures from bio-mimetic approaches in flow control will be discussed in the presentation.

  10. Flow between eccentric cylinders: a shear-extensional controllable flow

    NASA Astrophysics Data System (ADS)

    Tian, Guoqiang; Wang, Mengmeng; Wang, Xiaolin; Jin, Gang

    2016-05-01

    In this work the non-Newtonian fluid between eccentric cylinders is simulated with finite element method. The flow in the annular gap between the eccentric rotating cylinders was found to be a shear-extensional controllable flow. The influence of rotating speed, eccentricity as well as the radius ratio on the extensional flow in the vicinity of the minimum gap between the inner and outer cylinder was quantitatively investigated. It was found that both the strengths of shear flow and extensional flow could be adjusted by changing the rotating speed. In respect to extensional flow, it was also observed that the eccentricity and radius ratio exert significant influences on the ratio of extensional flow. And it should be noted that the ratio of extensional flow in the mix flow could be increased when increasing the eccentricity and the ratio of shear flow in the mix flow could be increased when increasing the radius ratio.

  11. Mechanobiological oscillators control lymph flow

    PubMed Central

    Kunert, Christian; Baish, James W.; Liao, Shan; Padera, Timothy P.; Munn, Lance L.

    2015-01-01

    The ability of cells to sense and respond to physical forces has been recognized for decades, but researchers are only beginning to appreciate the fundamental importance of mechanical signals in biology. At the larger scale, there has been increased interest in the collective organization of cells and their ability to produce complex, “emergent” behaviors. Often, these complex behaviors result in tissue-level control mechanisms that manifest as biological oscillators, such as observed in fireflies, heartbeats, and circadian rhythms. In many cases, these complex, collective behaviors are controlled—at least in part—by physical forces imposed on the tissue or created by the cells. Here, we use mathematical simulations to show that two complementary mechanobiological oscillators are sufficient to control fluid transport in the lymphatic system: Ca2+-mediated contractions can be triggered by vessel stretch, whereas nitric oxide produced in response to the resulting fluid shear stress causes the lymphatic vessel to relax locally. Our model predicts that the Ca2+ and NO levels alternate spatiotemporally, establishing complementary feedback loops, and that the resulting phasic contractions drive lymph flow. We show that this mechanism is self-regulating and robust over a range of fluid pressure environments, allowing the lymphatic vessels to provide pumping when needed but remain open when flow can be driven by tissue pressure or gravity. Our simulations accurately reproduce the responses to pressure challenges and signaling pathway manipulations observed experimentally, providing an integrated conceptual framework for lymphatic function. PMID:26283382

  12. Control of oscillator and amplifier flows

    NASA Astrophysics Data System (ADS)

    Schmid, Peter

    2012-11-01

    Flow control aims at the targeted manipulation of inherent flow behavior and is a critical component in efforts to delay instabilities, reduce drag, decrease receptivities or extend the operational parameter range of a fluid device. The design of flow control strategies relies on a model for the fluid system but also a model for the noise environment. For flows that are insensitive to external noise (oscillator flows), effective control strategies have been designed with considerable success; for flows that respond sensitively to environmental noise (amplifier flows), however, the design of effective control schemes is far more challenging, as it crucially depends on the quality of the noise model. We will present and discuss the critical steps in the design of flow control schemes for both types of flow behavior and compare and contrast a model based and data-based approach. This presentation summarizes joint work with Denis Sipp (ONERA-DAFE) and various doctoral students.

  13. Micropatterned fluid lipid bilayer arrays created using a continuous flow microspotter.

    PubMed

    Smith, Kathryn A; Gale, Bruce K; Conboy, John C

    2008-11-01

    We have developed a new method for creating micropatterned lipid bilayer arrays (MLBAs) using a 3D microfluidic system. An array of fluid lipid membranes was patterned onto a glass substrate using a Continuous Flow Microspotter. Fluorescence microscopy experiments were used to verify the formation of a bilayer structure on the glass substrate. Fluorescence recovery after photobleaching experiments demonstrated the bilayers' fluidity was maintained while being individually corralled on the substrate. The reproducibility of bilayer formation within an array was demonstrated by the linear response of membrane fluorescence versus mol % rhodamine functionalized lipids incorporated into the vesicles prior to fusion to the surface. The highly customizable nature of the MLBAs was demonstrated utilizing three different fluorescently labeled lipids to generate a multiple component lipid array. Finally, the cholera toxin B/ganglioside GM 1, antidinitrophenyl (DNP) antibody/DNP, and NeutrAvidin/biotin protein-ligand systems were used to model multiple protein-ligand binding on the MLBAs. The multicomponent patterned bilayers were functionalized with GM 1, DNP, and biotin lipids, and binding curves was generated by recording surface fluorescence versus increasing concentration of membrane bound ligands. PMID:18841940

  14. Micropatterned Fluid Lipid Bilayer Arrays Created Using a Continuous Flow Microspotter

    PubMed Central

    Smith, Kathryn A.; Gale, Bruce K.; Conboy, John C.

    2010-01-01

    We have developed a new method for creating micropatterned lipid bilayer arrays (MLBAs) using a 3D microfluidic system. An array of fluid lipid membranes was patterned onto a glass substrate using a Continuous Flow Microspotter™ (CFM). Fluorescence microscopy experiments were used to verify the formation of a bilayer structure on the glass substrate. Fluorescence recovery after photobleaching (FRAP) experiments demonstrated the bilayers fluidity was maintained while being individually corralled on the substrate. The reproducibility of bilayer formation within an array was demonstrated by the linear response of membrane fluorescence versus mol % rhodamine functionalized lipids incorporated into the vesicles prior to fusion to the surface. The highly customizable nature of the MLBAs was demonstrated utilizing three different fluorescently labeled lipids to generate a multiple component lipid array. Finally, the cholera toxin B (CTB)/ganglioside GM1, anti-dinitrophenyl (DNP) antibody/DNP and NeutrAvidin/biotin protein-ligand systems were used to model multiple protein-ligand binding on the MLBAs. The multi-component patterned bilayers were functionalized with GM1, DNP and biotin lipids and binding curves was generated by recording surface fluorescence versus increasing concentration of membrane bound ligands. PMID:18841940

  15. Circulating lipids and glycaemic control in insulin dependent diabetic children.

    PubMed Central

    Azad, K; Parkin, J M; Court, S; Laker, M F; Alberti, K G

    1994-01-01

    The prevalence of dyslipidaemia in children with insulin dependent diabetes mellitus (IDDM) and its relation to glycaemic control was studied in a group of 51 diabetic children and a control population of 132 schoolchildren. The prevalence of dyslipidaemia in the fasting state was increased in the diabetic group (39%) compared with control subjects (17%). Serum cholesterol concentration alone was raised in 25% of diabetic subjects while serum cholesterol and triglycerides were raised in 14%, compared with 16% and 0.7% respectively in control subjects. Serum total cholesterol (5.1 v 4.5 mmol/l), low density lipoprotein cholesterol (3.2 v 2.6 mmol/l), non-esterified fatty acids (0.91 v 0.50 mmol/l), and triglycerides (0.94 v 0.76 mmol/l) were higher in diabetic children. Serum total cholesterol, triglycerides, and apolipoprotein (apo)B concentrations increased with worsening control, while serum high density lipoprotein cholesterol and apoA-I concentrations were unaltered. There were also positive correlations between glycated haemoglobin and total cholesterol, triglycerides, and apoB in diabetic children. Thus, abnormalities in circulating lipids are common in young subjects with IDDM but largely disappear if blood glucose concentrations are reasonably controlled. PMID:7944528

  16. Autophagy regulation depends on ER homeostasis controlled by lipid droplets.

    PubMed

    Velázquez, Ariadna P; Graef, Martin

    2016-08-01

    Macroautophagy (hereafter autophagy) is a highly conserved homeostasis and quality control process critically linked to neurodegeneration, metabolic diseases, cancer, and aging. A key feature of autophagy is the de novo formation of autophagosomes, double-membrane vesicular structures encapsulating cytoplasmic cargo for vacuolar turnover and recycling. The membrane rearrangements underlying nucleation, expansion, closure, and vacuolar fusion of autophagosomes are driven by multicomponent core autophagy machinery in cooperation with numerous factors involved in a variety of cellular processes. Our current understanding of the origin and contribution of diverse membrane sources to autophagosome biogenesis and of cellular functions enabling stress-appropriate autophagy responses critical for cell health and survival remains limited. Here, we summarize and discuss our recent findings analyzing the role of lipid droplets (LDs), conserved intracellular storage compartments for neutral lipids, for autophagy regulation. Our data indicate that LDs are dispensable as membrane sources, but fulfill critical functions for maintaining endoplasmic reticulum (ER) homeostasis, including buffering of newly synthesized fatty acids and maintenance of phospholipid composition, required for intact autophagy regulation and cell survival during nutrient stress. PMID:27245853

  17. Controlled Transport of Functionalized Nanochannel though Lipid Membrane

    NASA Astrophysics Data System (ADS)

    Dutt, Meenakshi; Kuksenok, Olga; Balazs, Anna C.

    2012-02-01

    Via the Dissipative Particle Dynamics approach, we study the directed transport of a transmembrane nanochannel to a desired location within a lipid bilayer. Each nanochannel encompasses an ABA architecture, with a hydrophobic shaft (B) with two hydrophilic ends (A). One of the ends of the nanochannel is functionalized with hydrophilic functional groups, or hairs. The hydrophilic hairs serve a dual role: (a) control transport across the membrane barrier, and (b) enable the channel relocation to a specific membrane site. Our system comprises a lipid membrane with an embedded transmembrane nanochannel with the hairs extending into solution. First, we hold a suitably functionalized pipette above the membrane while the nanochannel freely diffuses within the membrane. For an optimal range of parameters, we demonstrate that the hairs find the pipette and spontaneously anchor onto it. We then show that by moving the pipette for a range of velocities, we can effectively transport the channel to any location within the membrane. This prototype assembly can provide guidelines for designing a number of systems for biomimetic applications.

  18. Power flow control using quadrature boosters

    NASA Astrophysics Data System (ADS)

    Sadanandan, Sandeep N.

    A power system that can be controlled within security constraints would be an advantage to power planners and real-time operators. Controlling flows can lessen reliability issues such as thermal limit violations, power stability problems, and/or voltage stability conditions. Control of flows can also mitigate market issues by reducing congestion on some lines and rerouting power to less loaded lines or onto preferable paths. In the traditional control of power flows, phase shifters are often used. More advanced methods include using Flexible AC Transmission System (FACTS) Controllers. Some examples include Thyristor Controlled Series Capacitors, Synchronous Series Static Compensators, and Unified Power Flow Controllers. Quadrature Boosters (QBs) have similar structures to phase-shifters, but allow for higher voltage magnitude during real power flow control. In comparison with other FACTS controllers QBs are not as complex and not as expensive. The present study proposes to use QBs to control power flows on a power system. With the inclusion of QBs, real power flows can be controlled to desired scheduled values. In this thesis, the linearized power flow equations used for power flow analysis were modified for the control problem. This included modifying the Jacobian matrix, the power error vector, and calculating the voltage injected by the quadrature booster for the scheduled real power flow. Two scenarios were examined using the proposed power flow control method. First, the power flow in a line in a 5-bus system was modified with a QB using the method developed in this thesis. Simulation was carried out using Matlab. Second, the method was applied to a 30-bus system and then to a 118-bus system using several QBs. In all the cases, the calculated values of the QB voltages led to desired power flows in the designated line.

  19. Lycopene control of benzophenone-sensitized lipid peroxidation

    NASA Astrophysics Data System (ADS)

    Cvetković, Dragan; Marković, Dejan

    2012-05-01

    Lycopene antioxidant activity in the presence of two different mixtures of phospholipids in hexane solution, under continuous regime of UV-irradiation from three different ranges (UV-A, UV-B, and UV-C) has been evaluated in this work. Lycopene expected role was to control lipid peroxidation, by scavenging free radicals generated by UV-irradiation, in the presence and in the absence of selected photosensitizer, benzophenone. This work shows that lycopene undergoes to UV-induced destruction (bleaching), highly dependent on the incident photons energy input, more expressed in the presence than in the absence of benzophenone. The further increase ("excess") of its bleaching is undoubtedly related to the further increase of its antioxidant activity in the presence of benzophenone, having the same cause: increase of (phospholipids peroxidation) chain-breaking activities.

  20. Controlling Flows Of Two Ingredients For Spraying

    NASA Technical Reports Server (NTRS)

    Chandler, Huel H.

    1995-01-01

    Closed-loop servo control subsystem incorporated, as modification, into system controlling flows of two ingredients mixed and sprayed to form thermally insulating foams on large tanks. Provides steady flows at specified rates. Foams produced smoother and of higher quality. Continued use of system results in substantial reduction in cost stemming from close control of application of foam and consequent reduced use of material.

  1. Development report, mass flow controller PN 5716068

    NASA Technical Reports Server (NTRS)

    Taylor, W.

    1972-01-01

    The design, development, and manufacture of an all mechanical mass flow controller are discussed. A test program was conducted using inert gas as the test medium. The unit controlled the pressure within plus of minus one percent. An analytical method is presented for relating the control pressure error with error in mass flow.

  2. Valve for controlling solids flow

    DOEpatents

    Feldman, David K.

    1980-01-01

    A fluidized solids control valve is disclosed that is particularly well adapted for use with a flow of coal or char that includes both large particles and fines. The particles may or may not be fluidized at various times during the operation. The valve includes a tubular body that terminates in a valve seat covered by a normally closed closure plate. The valve body at the seat and the closure plate is provided with aligned longitudinal slots that receive a pivotally supported key plate. The key plate is positionable by an operator in inserted, intermediate and retracted positions respecting the longitudinal slot in the valve body. The key plate normally closes the slot within the closure plate but is shaped and aligned obliquely to the longitudinal slot within the valve body to provide progressively increasing slot openings between the inserted and retracted positions. Transfer members are provided between the operator, key plate and closure plate to move the closure plate into an open position only when the key plate is retracted from the longitudinal slot within the valve body.

  3. LAM actuated propellant flow control device

    NASA Astrophysics Data System (ADS)

    Reinicke, Robert H.; Cust, Kevin M.

    1992-02-01

    An advanced design LAM (limited angle motor) positions an integral flow control element for bi-level flow control of storable propellants. The LAM incorporates permanent magnet latching to maintain the flow control element in either the low or high flow position without continuous electrical energization. The LAM stator and rotor are fully sheathed within stainless steel. This construction method permits the LAM to control storable propellants without using dynamic seals to isolate the LAM from the propellants. All welded construction prevents external leakage. The design concept selection rationale and the computer FEA (finite element analysis) methods employed to optimize design characteristics are presented. Correlations of analyses to test results are described.

  4. Identification and Control of Separated Shear Flows

    NASA Astrophysics Data System (ADS)

    Huang, Shao-Ching; Kim, John

    2002-11-01

    There has been increased interest in applying modern control theory to flow-control problems. For simple flows, such as turbulent channel and boundary layers, several investigators have constructed controllers based on linear optimal control theory, which requires certain information of the system to be controlled. However, for complex flows, such as separated flow past an airfoil, the required system information is not readily available, thus hindering the construction of controllers following the same procedure used for the simple flows. In this study, we use the system identification theory to construct a model of flow system for controller design. The model, as an approximation to the actual system, is based on the input-output relationship of the actual system. The locations of sensors and actuators are determined based on the spatial and temporal correlations of the flow field and practical measurement considerations. The system identification approach has been applied to both simple and complex flows. Linear and nonlinear disturbances to selected flow systems are considered to evaluate the performance of the constructed model. A series of numerical experiments have been performed to assess the validity of using linear approximations for nonlinear complex flows.

  5. Successes and Challenges for Flow Control Simulations

    NASA Technical Reports Server (NTRS)

    Rumsey, Christopher L.

    2008-01-01

    A survey is made of recent computations published for synthetic jet flow control cases from a CFD workshop held in 2004. The three workshop cases were originally chosen to represent different aspects of flow control physics: nominally 2-D synthetic jet into quiescent air, 3-D circular synthetic jet into turbulent boundary-layer crossflow, and nominally 2-D flow-control (both steady suction and oscillatory zero-net-mass-flow) for separation control on a simple wall-mounted aerodynamic hump shape. The purpose of this survey is to summarize the progress as related to these workshop cases, particularly noting successes and remaining challenges for computational methods. It is hoped that this summary will also by extension serve as an overview of the state-of-the-art of CFD for these types of flow-controlled flow fields in general.

  6. Successes and Challenges for Flow Control Simulations

    NASA Technical Reports Server (NTRS)

    Rumsey, Christopher L.

    2008-01-01

    A survey is made of recent computations published for synthetic jet flow control cases from a CFD workshop held in 2004. The three workshop cases were originally chosen to represent different aspects of flow control physics: nominally 2-D synthetic jet into quiescent air, 3-D circular synthetic jet into turbulent boundarylayer crossflow, and nominally 2-D flow-control (both steady suction and oscillatory zero-net-mass-flow) for separation control on a simple wall-mounted aerodynamic hump shape. The purpose of this survey is to summarize the progress as related to these workshop cases, particularly noting successes and remaining challenges for computational methods. It is hoped that this summary will also by extension serve as an overview of the state-of-the-art of CFD for these types of flow-controlled flow fields in general.

  7. CFD Modeling for Active Flow Control

    NASA Technical Reports Server (NTRS)

    Buning, Pieter G.

    2001-01-01

    This presentation describes current work under UEET Active Flow Control CFD Research Tool Development. The goal of this work is to develop computational tools for inlet active flow control design. This year s objectives were to perform CFD simulations of fully gridded vane vortex generators, micro-vortex genera- tors, and synthetic jets, and to compare flowfield results with wind tunnel tests of simple geometries with flow control devices. Comparisons are shown for a single micro-vortex generator on a flat plate, and for flow over an expansion ramp with sidewall effects. Vortex core location, pressure gradient and oil flow patterns are compared between experiment and computation. This work lays the groundwork for evaluating simplified modeling of arrays of devices, and provides the opportunity to test simple flow control device/sensor/ control loop interaction.

  8. Local Control of Blood Flow

    ERIC Educational Resources Information Center

    Clifford, Philip S.

    2011-01-01

    Organ blood flow is determined by perfusion pressure and vasomotor tone in the resistance vessels of the organ. Local factors that regulate vasomotor tone include myogenic and metabolic autoregulation, flow-mediated and conducted responses, and vasoactive substances released from red blood cells. The relative importance of each of these factors…

  9. How PI3K-derived lipids control cell division

    PubMed Central

    Campa, Carlo C.; Martini, Miriam; De Santis, Maria C.; Hirsch, Emilio

    2015-01-01

    To succeed in cell division, intense cytoskeletal and membrane remodeling are required to allow accurate chromosome segregation and cytoplasm partitioning. Spatial restriction of the actin dynamics and vesicle trafficking define the cell symmetry and equivalent membrane scission events, respectively. Protein complexes coordinating mitosis are recruited to membrane microdomains characterized by the presence of the phosphatidylinositol lipid members (PtdIns), like PtdIns(3,4,5)P3,PtdIns(4,5)P2, and PtdIns(3)P. These PtdIns represent a minor component of cell membranes, defining membrane domain identity, ultimately controlling cytoskeleton and membrane dynamics during mitosis. The coordinated presence of PtdIns(3,4,5)P3 at the cell poles and PtdIns(4,5)P2 at the cleavage furrow controls the polarity of the actin cytoskeleton leading to symmetrical cell division. In the endosomal compartment, the trafficking of PtdIns(3)P positive vesicles allows the recruitment of the protein machinery required for the abscission. PMID:26484344

  10. Energy conservation with automatic flow control valves

    SciTech Connect

    Phillips, D.

    1984-12-01

    Automatic flow control valves are offered in a wide range of sizes starting at 1/2 in. with flow rates of 0.5 gpm and up. They are also provided with materials and end connections to meet virtually any fan-coil system requirement. Among these are copper sweat type valves; ductile iron threaded valves; male/female threaded brass valves; and combination flow control/ball valves with union ends.

  11. Boundary-Layer-Ingesting Inlet Flow Control

    NASA Technical Reports Server (NTRS)

    Owens, Lewis R.; Allan, Brian G.; Gorton, Susan A.

    2006-01-01

    This paper gives an overview of a research study conducted in support of the small-scale demonstration of an active flow control system for a boundary-layer-ingesting (BLI) inlet. The effectiveness of active flow control in reducing engine inlet circumferential distortion was assessed using a 2.5% scale model of a 35% boundary-layer-ingesting flush-mounted, offset, diffusing inlet. This experiment was conducted in the NASA Langley 0.3-meter Transonic Cryogenic Tunnel at flight Mach numbers with a model inlet specifically designed for this type of testing. High mass flow actuators controlled the flow through distributed control jets providing the active flow control. A vortex generator point design configuration was also tested for comparison purposes and to provide a means to examine a hybrid vortex generator and control jets configuration. Measurements were made of the onset boundary layer, the duct surface static pressures, and the mass flow through the duct and the actuators. The distortion and pressure recovery were determined by 40 total pressure measurements on 8 rake arms each separated by 45 degrees and were located at the aerodynamic interface plane. The test matrix was limited to a maximum free-stream Mach number of 0.85 with scaled mass flows through the inlet for that condition. The data show that the flow control jets alone can reduce circumferential distortion (DPCP(sub avg)) from 0.055 to about 0.015 using about 2.5% of inlet mass flow. The vortex generators also reduced the circumferential distortion from 0.055 to 0.010 near the inlet mass flow design point. Lower inlet mass flow settings with the vortex generator configuration produced higher distortion levels that were reduced to acceptable levels using a hybrid vortex generator/control jets configuration that required less than 1% of the inlet mass flow.

  12. Boundary-Layer-Ingesting Inlet Flow Control

    NASA Technical Reports Server (NTRS)

    Owens, Lewis R.; Allan, Brian G.; Gorton, Susan A.

    2006-01-01

    This paper gives an overview of a research study conducted in support of the small-scale demonstration of an active flow control system for a boundary-layer-ingesting (BLI) inlet. The effectiveness of active flow control in reducing engine inlet circumferential distortion was assessed using a 2.5% scale model of a 35% boundary-layer-ingesting flush-mounted, offset, diffusing inlet. This experiment was conducted in the NASA Langley 0.3-meter Transonic Cryogenic Tunnel at flight Mach numbers with a model inlet specifically designed for this type of testing. High mass flow actuators controlled the flow through distributed control jets providing the active flow control. A vortex generator point design configuration was also tested for comparison purposes and to provide a means to examine a hybrid vortex generator and control jets configuration. Measurements were made of the onset boundary layer, the duct surface static pressures, and the mass flow through the duct and the actuators. The distortion and pressure recovery were determined by 40 total pressure measurements on 8 rake arms each separated by 45 degrees and were located at the aerodynamic interface plane. The test matrix was limited to a maximum free-stream Mach number of 0.85 with scaled mass flows through the inlet for that condition. The data show that the flow control jets alone can reduce circumferential distortion (DPCPavg) from 0.055 to about 0.015 using about 2.5% of inlet mass flow. The vortex generators also reduced the circumferential distortion from 0.055 to 0.010 near the inlet mass flow design point. Lower inlet mass flow settings with the vortex generator configuration produced higher distortion levels that were reduced to acceptable levels using a hybrid vortex generator/control jets configuration that required less than 1% of the inlet mass flow.

  13. Flow Control in a Compact Inlet

    NASA Astrophysics Data System (ADS)

    Vaccaro, John C.

    2011-12-01

    An experimental investigation of flow control, via various control jets actuators, was undertaken to eliminate separation and secondary flows in a compact inlet. The compact inlet studied was highly aggressive with a length-to-diameter ratio of 1.5. A brand new facility was designed and built to enable various actuation methodologies as well as multiple measurement techniques. Techniques included static surface pressure, total pressure, and stereoscopic particle image velocimetry. Experimental data were supplemented with numerical simulations courtesy of Prof. Kenneth Jansen, Dr. Onkar Sahni, and Yi Chen. The baseline flow field was found to be dominated by two massive separations and secondary flow structures. These secondary structures were present at the aerodynamic interface plane in the form of two counter-rotating vortices inducing upwash along centerline. A dominant shedding frequency of 350 Hz was measured both at the aerodynamic interface plane and along the lower surface of the inlet. Flow control experiments started utilizing a pair of control jets placed in streamwise locations where flow was found to separate. Tests were performed for a range of inlet Mach numbers from 0.2 to 0.44. Steady and unsteady static pressure measurements along the upper and lower walls of the duct were performed for various combinations of actuation. The parameters that were tested include the control jets momentum coefficient, their blowing ratio, the actuation frequency, as well as different combinations of jets. It was shown that using mass flux ratio as a criterion to define flow control is not sufficient, and one needs to provide both the momentum coefficient and the blowing ratio to quantify the flow control performance. A detailed study was undertaken on controlling the upstream separation point for an inlet Mach number of 0.44. Similar to the baseline flow field, the flow field associated with the activation of a two-dimensional control jet actuator was dominated by

  14. pH-Cleavable Nucleoside Lipids: A New Paradigm for Controlling the Stability of Lipid-Based Delivery Systems.

    PubMed

    Oumzil, Khalid; Benizri, Sébastien; Tonelli, Giovanni; Staedel, Cathy; Appavoo, Ananda; Chaffanet, Max; Navailles, Laurence; Barthélémy, Philippe

    2015-11-01

    Lipid-based delivery systems are an established technology with considerable clinical acceptance and several applications in human. Herein, we report the design, synthesis and evaluation of novel orthoester nucleoside lipids (ONLs) for the modulation of liposome stability. The ONLs contain head groups with 3'-orthoester nucleoside derivatives featuring positive or negative charges. The insertion of the orthoester function in the NL structures allows the formation of pH-sensitive liposomes. ONL-based liposomes can be hydrolyzed to provide nontoxic products, including nucleoside derivatives and hexadecanol. To allow the release to be tunable at different hydrolysis rates, the charge of the polar head structure is modulated, and the head group can be released at a biologically relevant pH. Crucially, when ONLs are mixed with natural phosphocholine lipids (PC), the resultant liposome evolves toward the formation of a hexadecanol/PC lamellar system. Biological evaluation shows that stable nucleic acid lipid particles (SNALPs) formulated with ONLs and siRNAs can effectively enter into tumor cells and release their nucleic acid payload in response to an intracellular acidic environment. This results in a much higher antitumor activity than conventional SNALPs. The ability to use pH-cleavable nucleolipids to control the stability of lipid-based delivery systems represents a promising approach for the intracellular delivery of drug cargos. PMID:26381578

  15. Power flow control using distributed saturable reactors

    DOEpatents

    Dimitrovski, Aleksandar D.

    2016-02-13

    A magnetic amplifier includes a saturable core having a plurality of legs. Control windings wound around separate legs are spaced apart from each other and connected in series in an anti-symmetric relation. The control windings are configured in such a way that a biasing magnetic flux arising from a control current flowing through one of the plurality of control windings is substantially equal to the biasing magnetic flux flowing into a second of the plurality of control windings. The flow of the control current through each of the plurality of control windings changes the reactance of the saturable core reactor by driving those portions of the saturable core that convey the biasing magnetic flux in the saturable core into saturation. The phasing of the control winding limits a voltage induced in the plurality of control windings caused by a magnetic flux passing around a portion of the saturable core.

  16. Cylinder Flow Control Using Plasma Actuators

    NASA Astrophysics Data System (ADS)

    Kozlov, Alexey; Thomas, Flint

    2007-11-01

    In this study the results of flow control experiments utilizing single dielectric barrier discharge plasma actuators to control flow separation and unsteady vortex shedding from a circular cylinder in cross-flow are reported. Two optimized quartz dielectric plasma actuators mounted on the cylinder surface utilizing an improved saw-tooth waveform high-voltage generator allowed flow control at Reynolds number approaching supercritical. Using either steady or unsteady actuation, it is demonstrated that the plasma-induced surface blowing gives rise to a local Coanda effect that promotes the maintenance of flow attachment. PIV based flow fields and wake velocity profiles obtained with hot-wire anemometry show large reductions in vortex shedding, wake width and turbulence intensity.

  17. Lipid self-assembled structures for reactivity control in food.

    PubMed

    Sagalowicz, L; Moccand, C; Davidek, T; Ghanbari, R; Martiel, I; Negrini, R; Mezzenga, R; Leser, M E; Blank, I; Michel, M

    2016-07-28

    Lipid self-assembled structures (SASs) have recently gained considerable interest for their potential applications, especially for sustained nutrient release and protein crystallization. An additional property, which is underexploited, is their ability to control chemical reactions in food products. Here, we concentrate on SASs formed by phospholipids (PLs) and monoglycerides (MGs), those compounds being the most natural surfactants and therefore, the best compatible with food products, in view of providing new functionalities through the formation of SASs. In this work, the phase behaviour of these amphiphiles when mixed with oil and water is described and compared. Subsequently, we address the influence of these structures to the oxidation and Maillard-type reactions. Finally, we show that SASs formed by MGs can strongly increase the yield of key aroma impact compounds generated by Maillard-type reactions when compared with the reaction performed in aqueous precursor solutions. Various SASs are compared. In particular, addition of oil to a reversed bicontinuous structure formed by MG leads to a reversed microemulsion, which, considering its low viscosity, is particularly suitable for food products and act as a very efficient reactor system. The influence of oil and precursors on phase behaviour is discussed and related to the efficiency of the Maillard reactions.This article is part of the themed issue 'Soft interfacial materials: from fundamentals to formulation'. PMID:27298441

  18. Transitioning Active Flow Control to Applications

    NASA Technical Reports Server (NTRS)

    Joslin, Ronald D.; Horta, Lucas G.; Chen, Fang-Jenq

    1999-01-01

    Active Flow Control Programs at NASA, the U.S. Air Force, and DARPA have been initiated with the goals of obtaining revolutionary advances in aerodynamic performance and maneuvering compared to conventional approaches. These programs envision the use of actuators, sensors, and controllers on applications such as aircraft wings/tails, engine nacelles, internal ducts, nozzles, projectiles, weapons bays, and hydrodynamic vehicles. Anticipated benefits of flow control include reduced weight, part count, and operating cost and reduced fuel burn (and emissions), noise and enhanced safety if the sensors serve a dual role of flow control and health monitoring. To get from the bench-top or laboratory test to adaptive distributed control systems on realistic applications, reliable validated design tools are needed in addition to sub- and large-scale wind-tunnel and flight experiments. This paper will focus on the development of tools for active flow control applications.

  19. Boundary-Layer-Ingesting Inlet Flow Control

    NASA Technical Reports Server (NTRS)

    Owens, Lewis R.; Allan, Brian G.; Gorton, Susan A.

    2008-01-01

    An experimental study was conducted to provide the first demonstration of an active flow control system for a flush-mounted inlet with significant boundary-layer-ingestion in transonic flow conditions. The effectiveness of the flow control in reducing the circumferential distortion at the engine fan-face location was assessed using a 2.5%-scale model of a boundary-layer-ingesting offset diffusing inlet. The inlet was flush mounted to the tunnel wall and ingested a large boundary layer with a boundary-layer-to-inlet height ratio of 35%. Different jet distribution patterns and jet mass flow rates were used in the inlet to control distortion. A vane configuration was also tested. Finally a hybrid vane/jet configuration was tested leveraging strengths of both types of devices. Measurements were made of the onset boundary layer, the duct surface static pressures, and the mass flow rates through the duct and the flow control actuators. The distortion and pressure recovery were measured at the aerodynamic interface plane. The data show that control jets and vanes reduce circumferential distortion to acceptable levels. The point-design vane configuration produced higher distortion levels at off-design settings. The hybrid vane/jet flow control configuration reduced the off-design distortion levels to acceptable ones and used less than 0.5% of the inlet mass flow to supply the jets.

  20. The digital control of anaesthetic gas flow.

    PubMed

    Boaden, R W; Hutton, P

    1986-04-01

    The theory and construction of a prototype digital gas flow controller are described. Using eight preset needle valves, it has the ability to deliver any flow from 50 to 12750 ml/minute in steps of 50 ml/minute. Under given conditions, the accuracy of this device is very high and its variation in performance with pipeline supply pressures is quantified. The required flow is requested from a BBC 'B' microcomputer which is interfaced with the equipment via a program written in Basic and the 1MHz bus port. The possible uses and potential of a microcomputer-controlled flow regulator in anaesthesia and intensive care are discussed. PMID:2939766

  1. Investigation of propellant flow control system

    NASA Technical Reports Server (NTRS)

    Liebman, A. A.

    1973-01-01

    Mechanical, electromechanical, and fluidic concepts were studied as propellant flow control system for oxygen/hydrogen attitude control thrusters. A mechanical flow controller was designed, fabricated, and tested with hydrogen, oxygen, and nitrogen over a range of inlet pressures and temperatures. Results of these tests are presented along with a discussion of a flight-weight design. Also presented are recommendations for further design and development. A detailed coverage of the fluidics investigation is included.

  2. Subsonic Flows through S-Ducts with Flow Control

    NASA Astrophysics Data System (ADS)

    Chen, Yi

    An inlet duct of an aircraft connects the air intake mounted on the fuselage to the engine within the aircraft body. The ideal outflow quality of the duct is steady, uniform and of high total pressure. Recently compact S-shaped inlet ducts are drawing more attention in the design of UAVs with short propulsion system. Compact ducts usually involve strong streamwise adverse pressure gradient and transverse secondary flow, leading to large-scale harmful vortical structures in the outflow. To improve the outflow quality modern flow control techniques have to be applied. Before designing successful flow control methods a solid understanding of the baseline flow field with the duct is crucial. In this work the fundamental mechanism of how the three dimensional flow topology evolves when the relevant parameters such as the duct geometry and boundary layer thickness are varied, is studied carefully. Two distinct secondary-flow patterns are identified. For the first time the sensitivity of the flow topology to the inflow boundary layer thickness in long ducts is clearly addressed. The interaction between the transverse motion induced by the transverse pressure gradient and the streamwise separation is revealed as the crucial reason for the various flow patterns existing in short ducts. A non-symmetric flow pattern is identified for the first time in both experiments and simulations in short ducts in which the intensity of the streamwise separation and the transverse invasion are in the same order of magnitude. A theory of energy accumulation and solution bifurcation is used to give a reasonable explanation for this non-symmetry. After gaining the knowledge of where and how the harmful vortical structures are generated several flow control techniques are tested to achieve a better outflow quality. The analysis of the flow control cases also provides a deeper insight into the behavior of the three-dimensional flow within the ducts. The conventional separation control method

  3. Symbiodinium Genotypic and Environmental Controls on Lipids in Reef Building Corals

    PubMed Central

    Cooper, Timothy F.; Lai, Michael; Ulstrup, Karin E.; Saunders, Sandra M.; Flematti, Gavin R.; Radford, Ben; van Oppen, Madeleine J. H.

    2011-01-01

    Background Lipids in reef building corals can be divided into two classes; non-polar storage lipids, e.g. wax esters and triglycerides, and polar structural lipids, e.g. phospholipids and cholesterol. Differences among algal endosymbiont types are known to have important influences on processes including growth and the photobiology of scleractinian corals yet very little is known about the role of symbiont types on lipid energy reserves. Methodology/Principal Findings The ratio of storage lipid and structural lipid fractions of Scott Reef corals were determined by thin layer chromatography. The lipid fraction ratio varied with depth and depended on symbiont type harboured by two corals (Seriatopora hystrix and Pachyseris speciosa). S. hystrix colonies associated with Symbiodinium C1 or C1/C# at deep depths (>23 m) had lower lipid fraction ratios (i.e. approximately equal parts of storage and structural lipids) than those with Symbiodinium D1 in shallow depths (<23 m), which had higher lipid fraction ratios (i.e. approximately double amounts of storage relative to structural lipid). Further, there was a non-linear relationship between the lipid fraction ratio and depth for S. hystrix with a modal peak at ∼23 m coinciding with the same depth as the shift from clade D to C types. In contrast, the proportional relationship between the lipid fraction ratio and depth for P. speciosa, which exhibited high specificity for Symbiodinium C3 like across the depth gradient, was indicative of greater amounts of storage lipids contained in the deep colonies. Conclusions/Significance This study has demonstrated that Symbiodinium exert significant controls over the quality of coral energy reserves over a large-scale depth gradient. We conclude that the competitive advantages and metabolic costs that arise from flexible associations with divergent symbiont types are offset by energetic trade-offs for the coral host. PMID:21637826

  4. Control of Differentiation of a Mammary Cell Line by Lipids

    NASA Astrophysics Data System (ADS)

    Dulbecco, Renato; Bologna, Mauro; Unger, Michael

    1980-03-01

    A rat mammary cell line (LA7) undergoes spontaneous differentiation into domes due to production of specific inducers by the cells. Some of these inducers may be lipids, and we show that lipids regulate this differentiation as both inducers and inhibitors. One inhibitor is the tumor promoter tetradecanoyl-13 phorbol 12-acetate. The inducers are saturated fatty acids of two groups: butyric acid and acids with chain lengths from C13 to C16, especially myristic acid (C14). Other inducers are myristoyl and palmitoyl lysolecithins, myristic acid methyl ester, and two cationic detergents with a tetradecenyl chain. We propose that the lipids with a C14-C16 alkyl chain affect differentiation by recognizing specific receptors through their alkyl chains and that the effects obtained depend on the head groups. These lipids may be physiological regulators in the mammary gland.

  5. Local flow control for active building facades

    NASA Astrophysics Data System (ADS)

    Kaligotla, Srikar; Chen, Wayne; Glauser, Mark

    2010-11-01

    Existing building facade designs are for a passive and an impermeable shell to prevent migration of outdoor air into the building and to control heat transfers between the exterior environment and the building interior. An active facade that can respond in real time to changing environmental conditions like wind speed and direction, pollutant load, temperature, humidity and light can lower energy use and maximize occupant comfort. With an increased awareness of cost and environmental effects of energy use, cross or natural ventilation has become an attractive method to lower energy use. Separated flow regions around such buildings are undesirable due to high concentration of pollutants, especially if the vents or dynamic windows for cross ventilation are situated in these regions. Outside pollutant load redistribution through vents can be regulated via flow separation control to minimize transport of pollutants into the building. Flow separation has been substantially reduced with the application of intelligent flow control tools developed at Syracuse University for flow around "silo" (turret) like structures. Similar flow control models can be introduced into buildings with cross ventilation for local external flow separation control. Initial experiments will be performed for turbulent flow over a rectangular block (scaled to be a mid-rise building) that has been configured with dynamic vents and unsteady suction actuators in a wind tunnel at various wind speeds.

  6. Control analysis of lipid biosynthesis in tissue cultures from oil crops shows that flux control is shared between fatty acid synthesis and lipid assembly.

    PubMed Central

    Ramli, Umi S; Baker, Darren S; Quant, Patti A; Harwood, John L

    2002-01-01

    Top-Down (Metabolic) Control Analysis (TDCA) was used to examine, quantitatively, lipid biosynthesis in tissue cultures from two commercially important oil crops, olive (Olea europaea L.) and oil palm (Elaeis guineensis Jacq.). A conceptually simplified system was defined comprising two blocks of reactions: fatty acid synthesis (Block A) and lipid assembly (Block B), which produced and consumed, respectively, a common and unique system intermediate, cytosolic acyl-CoA. We manipulated the steady-state levels of the system intermediate by adding exogenous oleic acid and, using two independent assays, measured the effect of the addition on the system fluxes (J(A) and J(B)). These were the rate of incorporation of radioactivity: (i) through Block A from [1-(14)C]acetate into fatty acids and (ii) via Block B from [U-(14)C]glycerol into complex lipids respectively. The data showed that fatty acid formation (Block A) exerted higher control than lipid assembly (Block B) in both tissues with the following group flux control coefficients (C):(i) Oil palm: *C(J(TL))(BlkA)=0.64+/-0.05 and *C(J(TL))(BlkB)=0.36+/-0.05(ii) Olive: *C(J(TL))(BlkA)=0.57+/-0.10 and *C(J(TL))(BlkB)=0.43+/-0.10where *C indicates the group flux control coefficient over the lipid biosynthesis flux (J(TL)) and the subscripts BlkA and BlkB refer to defined blocks of the system, Block A and Block B. Nevertheless, because both parts of the lipid biosynthetic pathway exert significant flux control, we suggest strongly that manipulation of single enzyme steps will not affect product yield appreciably. The present study represents the first use of TDCA to examine the overall lipid biosynthetic pathway in any tissue, and its findings are of immediate academic and economic relevance to the yield and nutritional quality of oil crops. PMID:12023882

  7. Controlling Gas-Flow Mass Ratios

    NASA Technical Reports Server (NTRS)

    Morris, Brian G.

    1990-01-01

    Proposed system automatically controls proportions of gases flowing in supply lines. Conceived for control of oxidizer-to-fuel ratio in new gaseous-propellant rocket engines. Gas-flow control system measures temperatures and pressures at various points. From data, calculates control voltages for electronic pressure regulators for oxygen and hydrogen. System includes commercially available components. Applicable to control of mass ratios in such gaseous industrial processes as chemical-vapor depostion of semiconductor materials and in automotive engines operating on compressed natural gas.

  8. Numerical Investigation of Plasma Active Flow Control

    NASA Astrophysics Data System (ADS)

    Sun, Baigang; Li, Feng; Zhang, Shanshan; Wang, Jingyu; Zhang, Lijuan; Zhao, Erlei

    2010-12-01

    Based on the theory of EHD (electronhydrodynamic), a simplified volume force model is applied to simulation to analyze the traits of plasma flow control in flow field, in which the cold plasma is generated by a DBD (dielectric-barrier-discharge) actuator. With the para-electric action of volume force in electric field, acceleration characteristics of the plasma flow are investigated for different excitation intensities of RF (radio frequency) power for the actuator. Furthermore, the plasma acceleration leads to an asymmetric distribution of flow field, and hence induces the deflection of jet plume, then results in a significant deflection angle of 6.26° thrust-vectoring effect. It appears that the plasma flow control technology is a new tentative method for the thrust-vectoring control of a space vehicle.

  9. Flow Control in a Transonic Diffuser

    NASA Astrophysics Data System (ADS)

    Gartner, Jeremy; Amitay, Michael

    2014-11-01

    In some airplanes such as fighter jets and UAV, short inlet ducts replace the more conventional ducts due to their shorter length. However, these ducts are associated with low length-to-diameter ratio and low aspect ratio and, thus, experience massive separation and the presence of secondary flow structures. These flow phenomena are undesirable as they lead to pressure losses and distortion at the Aerodynamic Interface Plane (AIP), where the engine face is located. It causes the engine to perform with a lower efficiency as it would with a straight duct diffuser. Different flow control techniques were studied on the short inlet duct, with the goal to reattach the flow and minimize the distortions at the AIP. Due to the complex interaction between the separation and the secondary flow structures, the necessity to understand the flow mechanisms, and how to control them at a more fundamental level, a new transonic diffuser with an upper ramp and a straight floor was designed and built. The objective of this project is to explore the effectiveness of different flow control techniques in a high subsonic (up to Mach 0.8) diffuser, so that the quasi two-dimensional separation and the formation of secondary flow structure can be isolated using a canonical flow field. Supported by Northrop Grumman.

  10. Control of shear flows by artificial excitation

    NASA Technical Reports Server (NTRS)

    Rice, E. J.; Zaman, K. B. M. Q.

    1987-01-01

    Investigations involving artificial excitation of various shear flows are reviewed. Potential applications of excitation in flow control, e.g., in enhancing mixing, and in delaying transition and separation are discussed. An account is given of the current activities at NASA Lewis Research Center in this regard.

  11. High precision high flow range control valve

    DOEpatents

    McCray, J.A.

    1999-07-13

    A fluid control valve is described having a valve housing having first and second valve housing openings for the ingress and egress of fluid through the control valve. Disposed within a void formed by the control valve is a sleeve having at least one sleeve opening to permit the flow of fluid therethrough. A flow restricter travels within the sleeve to progressively block off the sleeve opening and thereby control flow. A fluid passageway is formed between the first valve housing opening and the outer surface of the sleeve. A second fluid passageway is formed between the inside of the sleeve and the second valve housing opening. Neither fluid passageway contains more than one 90 [degree] turn. In the preferred embodiment only one of the two fluid passageways contains a 90[degree] turn. In another embodiment, the control valve housing is bifurcated by a control surface having control surface opening disposed therethrough. A flow restricter is in slidable contact with the control surface to restrict flow of fluid through the control surface openings. 12 figs.

  12. High precision high flow range control valve

    DOEpatents

    McCray, John A.

    1999-01-01

    A fluid control valve is described having a valve housing having first and second valve housing openings for the ingress and egress of fluid through the control valve. Disposed within a void formed by the control valve is a sleeve having at least one sleeve opening to permit the flow of fluid therethrough. A flow restricter travels within the sleeve to progressively block off the sleeve opening and thereby control flow. A fluid passageway is formed between the first valve housing opening and the outer surface of the sleeve. A second fluid passageway is formed between the inside of the sleeve and the second valve housing opening. Neither fluid passageway contains more than one 90.degree. turn. In the preferred embodiment only one of the two fluid passageways contains a 90.degree. turn. In another embodiment, the control valve housing is bifurcated by a control surface having control surface opening disposed therethrough. A flow restricter is in slidable contact with the control surface to restrict flow of fluid through the control surface openings.

  13. Optimal feedback control of turbulent channel flow

    NASA Technical Reports Server (NTRS)

    Bewley, Thomas; Choi, Haecheon; Temam, Roger; Moin, Parviz

    1993-01-01

    Feedback control equations were developed and tested for computing wall normal control velocities to control turbulent flow in a channel with the objective of reducing drag. The technique used is the minimization of a 'cost functional' which is constructed to represent some balance of the drag integrated over the wall and the net control effort. A distribution of wall velocities is found which minimizes this cost functional some time shortly in the future based on current observations of the flow near the wall. Preliminary direct numerical simulations of the scheme applied to turbulent channel flow indicates it provides approximately 17 percent drag reduction. The mechanism apparent when the scheme is applied to a simplified flow situation is also discussed.

  14. Magnetic Amplifier for Power Flow Control

    SciTech Connect

    2012-02-24

    GENI Project: ORNL is developing an electromagnet-based, amplifier-like device that will allow for complete control over the flow of power within the electric grid. To date, complete control of power flow within the grid has been prohibitively expensive. ORNL’s controller could provide a reliable, cost-effective solution to this problem. The team is combining two types of pre-existing technologies to assist in flow control, culminating in a prototype iron-based magnetic amplifier. Ordinarily, such a device would require expensive superconductive wire, but the magnetic iron core of ORNL’s device could serve as a low-cost alternative that is equally adept at regulating power flow.

  15. Active Flow Control Stator With Coanda Surface

    NASA Technical Reports Server (NTRS)

    Guendogdu; Vorreiter; Seume

    2010-01-01

    Active Flow Control increases the permissible aerodynamic loading. Curved surface near the trailing edge ("Coanda surface"): a) increases turning -> higher pressure ratio. b) controls boundary layer separation -> increased surge margin. Objective: Reduce the number of vanes or compressor stages. Constraints: 1. In a real compressor, the vane must still function entirely without blowing. 2. Maintain the flow exit angle of the reference stator despite the resulting increase in stator loading.

  16. Value for controlling flow of cryogenic fluid

    DOEpatents

    Knapp, Philip A.

    1996-01-01

    A valve is provided for accurately controlling the flow of cryogenic fluids such as liquid nitrogen. The valve comprises a combination of disc and needle valves affixed to a valve stem in such a manner that the disc and needle are free to rotate about the stem, but are constrained in lateral and vertical movements. This arrangement provides accurate and precise fluid flow control and positive fluid isolation.

  17. Control and Identification of a Separated Flow

    NASA Astrophysics Data System (ADS)

    Huang, Shao-Ching; Kim, John; Gibson, Steve

    2004-11-01

    There has been increased interest in applying modern control theory to flow-control problems. For simple flows, such as turbulent channel and boundary layers, several investigators have successfully designed controllers based on linear optimal control theory. However, applying the same procedure to complex flows is hindered since certain required information of the flow is not readily available. In this study, we use system identification theory to construct an approximate linear model from input-output data for a separated boundary layer on a flat plate exposed to an adverse pressure gradient. The subspace system identification method yields a more accurate system model than the ARX method we had used previously. A linear quadratic Gaussian (LQG) synthesis was used to design an optimal controller to reduce the separation bubble size. Effects of the controller were investigated by comparing the controlled flow field to the uncontrolled one, and that controlled by a conventional open-loop scheme. A number of issues regarding model reduction, model stability, and the choice of cost function, will also be presented.

  18. Liquid-Flow Controller Responds To Pressure

    NASA Technical Reports Server (NTRS)

    Cox, George B., Jr.

    1990-01-01

    Mechanism controls flow of liquid in fuel-spraying head in combustion chamber responds nonlinearly to pressure of liquid. Shell of spraybar expands or contracts laterally as its internal pressure rises or falls, forcing collar down or up on entry tube. Area of window formed by slots in collar and entry tube thus increases or decreases. Drop in pressure through variable-area orifice increases much more with flow through orifice than does corresponding drop in pressure with flow through fixed-area orifice. In practical terms, lower pump pressure needed with variable orifice for given flow of liquid. Principle of operation applicable to spraying heads for other fluids.

  19. Control of Mixing and Reactive Flow Processes

    NASA Technical Reports Server (NTRS)

    Karagozian, A. R.

    1999-01-01

    The interdisciplinary field of reactive flow control is one that holds a great deal of promise for the optimization of complex phenomena occurring in many practical systems, ranging from automobile and gas turbine engines to environmental thermal destruction systems. The fundamental underpinnings of combustion control, however, require a detailed level of understanding of complex reactive flow phenomena, and, in the case of closed-loop active control, require the ability to sense (monitor) and actuate (manipulate) flow processes in a spatially distributed manner in "near real time". Hence the ultimate growth and success of the field of reactive flow control is intimately linked: 1) to advances in the understanding, simulation, and model reduction for complex reactive flows, 2) to the development of experimental diagnostic techniques, in particular, to the development of physically robust sensors, and 3) to the development of a framework or frameworks for generation of closed loop control algorithms suitable for unsteady, nonlinear reactive flow systems. The present paper seeks to outline the potential benefits and technical challenges that exist for mixing and combustion control in fundamental as well as practical systems and to identify promising research directions that could help meet these challenges.

  20. The art and science of flow control

    NASA Technical Reports Server (NTRS)

    Gad-El-hak, Mohamed

    1989-01-01

    The ability to actively or passively manipulate a flow field to effect a desired change is of immense technological importance. In this article, methods of control to achieve transition delay, separation postponement, lift enhancement, drag reduction, turbulence augmentation, or noise suppression are considered. Emphasis is placed on external boundary-layer flows although applicability of some of the methods reviewed for internal flows will be mentioned. Attempts will be made to present a unified view of the different methods of control to achieve a variety of end results. Performance penalties associated with a particular method such as cost, complexity, or trade-off will be elaborated.

  1. Variable flow control for a nuclear reactor control rod

    DOEpatents

    Carleton, Richard D.; Bhattacharyya, Ajay

    1978-01-01

    A variable flow control for a control rod assembly of a nuclear reactor that depends on turbulent friction though an annulus. The annulus is formed by a piston attached to the control rod drive shaft and a housing or sleeve fitted to the enclosure housing the control rod. As the nuclear fuel is burned up and the need exists for increased reactivity, the control rods are withdrawn, which increases the length of the annulus and decreases the rate of coolant flow through the control rod assembly.

  2. Monitoring And Controlling Hydroponic Flow

    NASA Technical Reports Server (NTRS)

    Dreschel, Thomas W.

    1992-01-01

    Pressure-monitoring and -controlling apparatus maintains slight suction required on nutrient solution in apparatus described in "Tubular Membrane Plant-Growth Unit" (KSC-11375), while overcoming gravity effects on operation of system on Earth. Suction helps to hold solution in tubular membrane.

  3. Method and apparatus for controlling fluid flow

    DOEpatents

    Miller, J.R.

    1980-06-27

    A method and apparatus for precisely controlling the rate (and hence amount) of fluid flow are given. The controlled flow rate is finely adjustable, can be extremely small (on the order of microliter-atmospheres per second), can be adjusted to zero (flow stopped), and is stable to better than 1% with time. The dead volume of the valve can be made arbitrarily small, in fact essentially zero. The valve employs no wearing mechanical parts (including springs, stems, or seals). The valve is finely adjustable, has a flow rate dynamic range of many decades, can be made compatible with any fluid, and is suitable for incorporation into an open or closed loop servo-control system.

  4. Veganism Is a Viable Alternative to Conventional Diet Therapy for Improving Blood Lipids and Glycemic Control.

    PubMed

    Trepanowski, John F; Varady, Krista A

    2015-01-01

    The American Diabetes Association (ADA) and the National Cholesterol Education Program (NCEP) have each outlined a set of dietary recommendations aimed at improving glycemic control and blood lipids, respectively. However, traditional vegan diets (low-fat diets that proscribe animal product consumption) are also effective at improving glycemic control, and dietary portfolios (vegan diets that contain prescribed amounts of plant sterols, viscous fibers, soy protein, and nuts) are also effective at improving blood lipids. The purpose of this review was to compare the effects of traditional vegan diets and dietary portfolios with ADA and NCEP diets on body weight, blood lipids, blood pressure, and glycemic control. The main findings are that traditional vegan diets appear to improve glycemic control better than ADA diets in individuals with type 2 diabetes mellitus (T2DM), while dietary portfolios have been consistently shown to improve blood lipids better than NCEP diets in hypercholesterolemic individuals. PMID:24922183

  5. [Uses of monomolecular lipid film for control of udo aphid (Toxoptera odinae (Van der Goot))].

    PubMed

    Chen, J; Cheng, H Z; Lin, Y L

    1993-02-01

    The biological character of udo aphid was observed and monomolecular lipid film was used to control it. The results showed that at 100 and 200 times the lipid film, the hatch rate of over-winter eggs was lower than the control by 30.76% and 12.15% respectively, and at 50, 100 and 200 times the lipid film the mortality of young nymph aphids was 100% and those of old nymph aphids and adult aphids were 8.31%, 32.62% and 3.31% respectively. PMID:8323704

  6. Thermal energy scavenger (flow control)

    SciTech Connect

    Hochstein, P.A.; Milton, H.W.; Pringle, W.L.

    1981-12-22

    A thermal energy scavenger assembly is described including a plurality of temperature-sensitive wires made of material which exhibits shape memory due to a thermoelastic, martensitic phase transformation. The wires are placed in tension between fixed and movable plates which are, in turn, supported by a pair of wheels which are rotatably supported by a housing for rotation about a central axis. A pair of upper and lower cams are fixed to the housing and cam followers react with the respective cams. Each cam transmits forces through a pair of hydraulic pistons. One of the pistons is connected to a movable plate to which one end of the wires are connected whereby a stress is applied to the wires to strain the wires during a first phase and whereby the cam responds to the unstraining of the wires during a second phase. A housing defines fluid compartments through which hot and cold fluid passes and flows radially through the wires whereby the wires become unstrained and shorten in length when subjected to the hot fluid for causing a reaction between the cam followers and the cams to effect rotation of the wheels about the central axis of the assembly, which rotation of the wheels is extracted through beveled gearing. The wires are grouped into a plurality of independent modules with each module having a movable plate, a fixed plate and the associated hydraulic pistons and cam follower. The hydraulic pistons and cam follower of a module are disposed at ends of the wires opposite from the ends of the wires at which the same components of the next adjacent modules are disposed so that the cam followers of alternate modules react with one of the cams and the remaining cam followers of the remaining modules react with the other cam. There is also including stress limiting means in the form of coil springs associated with alternate ends of the wires for limiting the stress or strain in the wires.

  7. Innovative Flow Control Concepts for Drag Reduction

    NASA Technical Reports Server (NTRS)

    Lin, John C.; Whalen, Edward A.; Eppink, Jenna L.; Siochi, Emilie J.; Alexander, Michael G.; Andino, Marlyn Y.

    2016-01-01

    This paper highlights the technology development of two flow control concepts for aircraft drag reduction. The NASA Environmentally Responsible Aviation (ERA) project worked with Boeing to demonstrate these two concepts on a specially outfitted Boeing 757 ecoDemonstrator during the spring of 2015. The first flow control concept used Active Flow Control (AFC) to delay flow separation on a highly deflected rudder and increase the side force that it generates. This may enable a smaller vertical tail to provide the control authority needed in the event of an engine failure during takeoff and landing, while still operating in a conventional manner over the rest of the flight envelope. Thirty-one sweeping jet AFC actuators were installed and successfully flight-tested on the vertical tail of the 757 ecoDemonstrator. Pilot feedback, flow cone visualization, and analysis of the flight test data confirmed that the AFC is effective, as a smoother flight and enhanced rudder control authority were reported. The second flow control concept is the Insect Accretion Mitigation (IAM) innovation where surfaces were engineered to mitigate insect residue adhesion on a wing's leading edge. This is necessary because something as small as an insect residue on the leading edge of a laminar flow wing design can cause turbulent wedges that interrupt laminar flow, resulting in an increase in drag and fuel use. Several non-stick coatings were developed by NASA and applied to panels that were mounted on the leading edge of the wing of the 757 ecoDemonstrator. The performance of the coated surfaces was measured and validated by the reduction in the number of bug adhesions relative to uncoated control panels flown simultaneously. Both flow control concepts (i.e., sweeping jet actuators and non-stick coatings) for drag reduction were the culmination of several years of development, from wind tunnel tests to flight tests, and produced valuable data for the advancement of modern aircraft designs

  8. Sphingolipids and Epoxidized Lipid Metabolites in the Control of Gut Immunosurveillance and Allergy

    PubMed Central

    Kunisawa, Jun; Kiyono, Hiroshi

    2016-01-01

    The intestinal immune system ingeniously balances the distinct responses of elimination and tolerance of non-self-substances for the creation and maintenance of homeostatic environments. Accumulating evidence has recently shown that various lipids, including dietary one, are involved in the regulation of intestinal immunity and are associated with biophylaxis and immune disorders. Recent advances in the lipidomics allow the identification of novel pathways of lipid metabolism and lipid metabolites for the control of intestinal immunity. In this paper, we describe the effects and functions of lipids, especially sphingolipids and new lipid metabolites originated from dietary oil on the immunomodulation and on the development and pathogenesis of allergic diseases in the intestine. PMID:26858949

  9. Sphingolipids and Epoxidized Lipid Metabolites in the Control of Gut Immunosurveillance and Allergy.

    PubMed

    Kunisawa, Jun; Kiyono, Hiroshi

    2016-01-01

    The intestinal immune system ingeniously balances the distinct responses of elimination and tolerance of non-self-substances for the creation and maintenance of homeostatic environments. Accumulating evidence has recently shown that various lipids, including dietary one, are involved in the regulation of intestinal immunity and are associated with biophylaxis and immune disorders. Recent advances in the lipidomics allow the identification of novel pathways of lipid metabolism and lipid metabolites for the control of intestinal immunity. In this paper, we describe the effects and functions of lipids, especially sphingolipids and new lipid metabolites originated from dietary oil on the immunomodulation and on the development and pathogenesis of allergic diseases in the intestine. PMID:26858949

  10. Analysis and control of cavity flow

    NASA Astrophysics Data System (ADS)

    Kourta, A.; Vitale, E.

    2008-07-01

    A flow above a cavity leads to an unsteady separated flow. This configuration exhibits an intense aeroacoustic coupling, where very intense aerodynamic noise can be emitted. Moreover, a majority of tangential flow above a cavity has an oscillatory character, resulting from a strong coupling between the acoustic and the flow dynamics. In the present work, we are interested in characterizing the dynamics and the frequency distribution of a cavity flow. First, the dynamics of the cavity are analyzed and the frequency distribution is established, which is followed by a study of nonlinear interaction. An open loop control using a synthetic jet is then applied in order to reduce noise generation. Finally, by choosing suitable jet parameters a significant noise reduction is obtained.

  11. RETROFITTING CONTROL FACILITIES FOR WET-WEATHER FLOW CONTROL

    EPA Science Inventory

    Available technologies were evaluated to demonstrate the feasibility and cost effectiveness of retrofitting existing facilities to handle wet-weather flow (WWF). Cost/benefit relationships were compared to construction of new conventional control and treatment facilities. Desktop...

  12. Bluff Body Flow Control Using Plasma Actuators

    NASA Astrophysics Data System (ADS)

    Thomas, Flint

    2005-11-01

    In this study, the use of single dielectric barrier discharge plasma actuators for the control of bluff body flow separation is investigated. In particular, surface mounted plasma actuators are used to reduce both drag and unsteady vortex shedding from circular cylinders in cross-flow. It is demonstrated that the plasma-induced surface blowing gives rise to a local Coanda effect that promotes the maintenance of flow attachment. Large reductions in vortex shedding and drag are demonstrated for Reynolds numbers ˜ 10^410^5. Both steady and unsteady plasma-induced surface blowing is explored. Results are presented from experiments involving both two and four surface mounted actuators.

  13. Lipid-lowering therapies, glucose control and incident diabetes: evidence, mechanisms and clinical implications.

    PubMed

    Zafrir, Barak; Jain, Mohit

    2014-08-01

    Lipid-lowering therapies constitute an essential part in the treatment and prevention of cardiovascular diseases and are consistently shown to reduce adverse cardiovascular outcomes in wide-scale populations. Recently, there is increased awareness of the possibility that lipid-lowering drugs may affect glucose control and insulin resistance. This phenomenon is reported in all classes of lipid-modifying agents, with differential effects of distinct drugs. Since the prevalence of metabolic syndrome and diabetes is rising, and lipid-modifying therapies are widely used to reduce the cardiovascular burden in these populations, it is of importance to examine the relationship between lipid-lowering drugs, glycemic control and incident diabetes. In the current review we discuss the evidence, ranging from experimental studies to randomized controlled clinical trials and meta-analyses, of how lipid-modifying therapies affect glycemic control and insulin sensitivity. Cumulative data suggest that both statins and niacin are associated with increased risk of impaired glucose control and development of new-onset diabetes, as opposed to bile-acid sequestrants which display concomitant moderate lipid and glucose lowering effects, and fibrates (particularly the pan-PPAR agonist bezafibrate) which may produce beneficial effects on glucose metabolism and insulin sensitivity. Ezetimibe is implied to ameliorate metabolic markers such as hepatic steatosis and insulin resistance, with yet little support from clinical trials, while fish oils which in experimental studies produce favorable effects on insulin sensitivity, although studied extensively, continue to show inconclusive effects on glucose homeostasis in patients with diabetes. Suggested mechanisms of how lipid-modifying agents affect glucose control and their clinical implications in this context, are summarized. PMID:24952127

  14. Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling.

    PubMed

    Jain, Madhulika; Petzold, Christopher J; Schelle, Michael W; Leavell, Michael D; Mougous, Joseph D; Bertozzi, Carolyn R; Leary, Julie A; Cox, Jeffery S

    2007-03-20

    Mycobacterium tuberculosis synthesizes specific polyketide lipids that interact with the host and are required for virulence. Using a mass spectrometric approach to simultaneously monitor hundreds of lipids, we discovered that the size and abundance of two lipid virulence factors, phthiocerol dimycocerosate (PDIM) and sulfolipid-1 (SL-1), are controlled by the availability of a common precursor, methyl malonyl CoA (MMCoA). Consistent with this view, increased levels of MMCoA led to increased abundance and mass of both PDIM and SL-1. Furthermore, perturbation of MMCoA metabolism attenuated pathogen replication in mice. Importantly, we detected increased PDIM synthesis in bacteria growing within host tissues and in bacteria grown in culture on odd-chain fatty acids. Because M. tuberculosis catabolizes host lipids to grow during infection, we propose that growth of M. tuberculosis on fatty acids in vivo leads to increased flux of MMCoA through lipid biosynthetic pathways, resulting in increased virulence lipid synthesis. Our results suggest that the shift to host lipid catabolism during infection allows for increased virulence lipid anabolism by the bacterium. PMID:17360366

  15. Vibrating surface actuators for active flow control

    NASA Astrophysics Data System (ADS)

    Calkins, Frederick T.; Clingman, Dan J.

    2002-07-01

    Current research has shown that aircraft can gain significant aerodynamic performance benefits from active flow control (AFC). AFC seeks to control large scale flows by exploiting natural response triggered by small energy inputs. The principal target application is download alleviation of the V-22 Osprey under the DARPA sponsored Boeing Active Flow Control System program. One method of injecting energy into the flow over the V22 wings is to use an active vibrating surface on the passive seal between the wing and flapperon. The active surface is an oscillating cantilevered beam which injects fluid into the flow, similar to a synthetic jet, and interacts with the flow field. Two types of actuators, or flipperons, are explored. The first is a multilayer piezoelectric polyvinylidene fluoride cantilevered bender. The second is a single crystal piezoelectric (SCP)d31 poled wafer mounted on a cantilevered spring steel substrate. This paper details the development effort including fabrication, mechanical and electrical testing, and modeling for both types of actuators. Both flipperons were mounted on the passive seal between a 1/10th scale V22 wing and flapperon and the aerodynamic performance evaluated in low speed wind tunnel. The SCP flipperon demonstrated significant cruise benefits, with increase of 10 percent lift and 20 percent angle of attack capability. The PVDF flipperon provided a 16 percent drag reduction in the hover mode.

  16. Optical control of electro-osmotic flow

    NASA Astrophysics Data System (ADS)

    Kirei, Huba; Der, Andras; Oroszi, Laszlo; Ferencz, Karpat; Rakovics, Vilmos; Ormos, Pal

    2005-08-01

    Electro-osmotic pumping is an efficient way to move fluids in microfluidic systems. It is driven by the interaction of the Debye layer formed in the vicinity of the charged channel wall with a tangential electric field. The key parameters that determine the flow properties are the zeta potential of the surface and the electric field that drives the flow. Consequently, the flow can be controlled by appropriately modifying these parameters. Controlling the charge on the channel wall makes it possible to modify fluid flow. Likewise, the electric field close to the surface can be modified by changing the conductivity of the surface. The surface charge of appropriate materials can be changed by light illumination: the application of this phenomenon offers the possibility to optically control flow parameters. We have tested this possibility with several light sensitive surfaces. In the class of materials that change their charge upon illumination TiO2, a well known photoactive material was investigated. Experiments were also performed with the protein bacteriorhodopsin, known to change its surface charge following the release of protons into the solvent upon illumination. CdS was tested as the photoconductive material to modify the electric field by light. Linear microfluidic channels were prepared by soft lithography: a PDMS mold was placed upon a planar glass surface so that a rectangular cross section channel was formed upon the glass. The photosensitive materials covered the bottom glass surface. The experiments show that the flow can be readily modulated by illumination. The results demonstrate that it is possible to dynamically control microfluidic flow, opening up the prospect to create optically controlled complex microfluidic networks.

  17. Xurography actuated valving for centrifugal flow control.

    PubMed

    Kinahan, David J; Early, Philip L; Vembadi, Abhishek; MacNamara, Eoghan; Kilcawley, Niamh A; Glennon, Thomas; Diamond, Dermot; Brabazon, Dermot; Ducrée, Jens

    2016-09-21

    We introduce a novel instrument controlled valving scheme for centrifugal platforms which is based upon xurography. In a first approach, which is akin to previously presented event-triggered flow control, the valves are composed of a pneumatic chamber sealed by a dissolvable film (DF) and by a pierceable membrane. Liquid is initially prevented from wetting the DF by the counter pressure of a trapped gas. Via a channel, this pocket is pneumatically connected to a vent, sealed by the pierceable membrane, located on the top surface of the disc. By scouring the top surface of the disc, along a pre-defined track by a robotic knife-cutter, the trapped gas is released and so the liquid can wet and disintegrate the DF. In order to automate assay protocols without the need to integrate DFs, we extend this xurography-based flow control concept by selective venting of chambers subjected to pneumatic over-pressure or vacuum suction. Unlike most instrument controlled flow-control mechanisms, in this approach to valve actuation can occur during disc rotation. To demonstrate the potential of this flow control approach, we designed a disc architecture to automate the liquid handling as the backbone of a biplex liver assay panel. We demonstrate valve actuation during rotation, using the robotic arm, using this disc with visualisation via dyed water. We then demonstrate the biplex liver assay, using calibration reagent, by stopping the disc and manually piercing the membrane to actuate the same valves. PMID:27523628

  18. Variable Frequency Diverter Actuation for Flow Control

    NASA Technical Reports Server (NTRS)

    Culley, Dennis E.

    2006-01-01

    The design and development of an actively controlled fluidic actuator for flow control applications is explored. The basic device, with one input and two output channels, takes advantage of the Coanda effect to force a fluid jet to adhere to one of two axi-symmetric surfaces. The resultant flow is bi-stable, producing a constant flow from one output channel, until a disturbance force applied at the control point causes the flow to switch to the alternate output channel. By properly applying active control the output flows can be manipulated to provide a high degree of modulation over a wide and variable range of frequency and duty cycle. In this study the momentary operative force is applied by small, high speed isolation valves of which several different types are examined. The active fluidic diverter actuator is shown to work in several configurations including that in which the operator valves are referenced to atmosphere as well as to a source common with the power stream.

  19. Method for controlling coolant flow in airfoil, flow control structure and airfoil incorporating the same

    DOEpatents

    Itzel, Gary Michael; Devine, II, Robert Henry; Chopra, Sanjay; Toornman, Thomas Nelson

    2003-07-08

    A coolant flow control structure is provided to channel cooling media flow to the fillet region defined at the transition between the wall of a nozzle vane and a wall of a nozzle segment, for cooling the fillet region. In an exemplary embodiment, the flow control structure defines a gap with the fillet region to achieve the required heat transfer coefficients in this region to meet part life requirements.

  20. An in situ optical imaging system for measuring lipid uptake, vessel contraction, and lymph flow in small animal lymphatic vessels

    NASA Astrophysics Data System (ADS)

    Kassis, Timothy; Weiler, Michael J.; Dixon, J. Brandon

    2012-03-01

    All dietary lipids are transported to venous circulation through the lymphatic system, yet the underlying mechanisms that regulate this process remain unclear. Understanding how the lymphatics functionally respond to changes in lipid load is important in the diagnosis and treatment of lipid and lymphatic related diseases such as obesity, hypercholesterolemia, and lymphedema. Therefore, we sought to develop an in situ imaging system to quantify and correlate lymphatic function as it relates to lipid transport. A custom-built optical set-up provides us with the capability of dual-channel imaging of both high-speed bright-field video and fluorescence simultaneously. This is achieved by dividing the light path into two optical bands. Utilizing high-speed and back-illuminated CCD cameras and post-acquisition image processing algorithms, we have the potential quantify correlations between vessel contraction, lymph flow and lipid concentration of mesenteric lymphatic vessels in situ. Local flow velocity is measured through lymphocyte tracking, vessel contraction through measurements of the vessel walls and lipid uptake through fluorescence intensity tracking of a fluorescent long chain fatty acid analogue, Bodipy FL C16. This system will prove to be an invaluable tool for both scientists studying lymphatic function in health and disease, and those investigating strategies for targeting the lymphatic system with orally delivered drugs.

  1. Development of flow through dielectrophoresis microfluidic chips for biofuel production: Sorting and detection of microalgae with different lipid contents

    PubMed Central

    Deng, Yu-Luen; Kuo, Mei-Yi; Juang, Yi-Je

    2014-01-01

    In this study, a continuous flow dielectrophoresis (DEP) microfluidic chip was fabricated and utilized to sort out the microalgae (C. vulgaris) with different lipid contents. The proposed separation scheme is to allow that the microalgae with different lipid contents experience different negative or no DEP force at the separation electrode pair under the pressure-driven flow. The microalgae that experience stronger negative DEP will be directed to the side channel while those experience less negative or no DEP force will pass through the separation electrode pair to remain in the main channel. It was found that the higher the lipid content inside the microalgae, the higher the crossover frequency. Separation of the microalgae with 13% and 21% lipid contents, and 24% and 30%–35% lipid contents was achieved at the operating frequency 7 MHz, and 10 MHz, respectively. Moreover, separation can be further verified by measurement of the fluorescence intensity of the neutral lipid inside the sorted algal cells. PMID:25553195

  2. Declarative flow control for distributed instrumentation

    SciTech Connect

    Parvin, Bahram; Taylor, John; Fontenay, Gerald; Callahan, Daniel

    2001-06-01

    We have developed a 'microscopy channel' to advertise a unique set of on-line scientific instruments and to let users join a particular session, perform an experiment, collaborate with other users, and collect data for further analysis. The channel is a collaborative problem solving environment (CPSE) that allows for both synchronous and asynchronous collaboration, as well as flow control for enhanced scalability. The flow control is a declarative feature that enhances software functionality at the experimental scale. Our testbed includes several unique electron and optical microscopes with applications ranging from material science to cell biology. We have built a system that leverages current commercial CORBA services, Web Servers, and flow control specifications to meet diverse requirements for microscopy and experimental protocols. In this context, we have defined and enhanced Instrument Services (IS), Exchange Services (ES), Computational Services (CS), and Declarative Services (DS) that sit on top of CORBA and its enabling services (naming, trading, security, and notification) IS provides a layer of abstraction for controlling any type of microscope. ES provides a common set of utilities for information management and transaction. CS provides the analytical capabilities needed for online microscopy. DS provides mechanisms for flow control for improving the dynamic behavior of the system.

  3. Adaptive muffler based on controlled flow valves.

    PubMed

    Šteblaj, Peter; Čudina, Mirko; Lipar, Primož; Prezelj, Jurij

    2015-06-01

    An adaptive muffler with a flexible internal structure is considered. Flexibility is achieved using controlled flow valves. The proposed adaptive muffler is able to adapt to changes in engine operating conditions. It consists of a Helmholtz resonator, expansion chamber, and quarter wavelength resonator. Different combinations of the control valves' states at different operating conditions define the main working principle. To control the valve's position, an active noise control approach was used. With the proposed muffler, the transmission loss can be increased by more than 10 dB in the selected frequency range. PMID:26093462

  4. Acoustic streaming flows and sample rotation control

    NASA Astrophysics Data System (ADS)

    Trinh, Eugene

    1998-11-01

    Levitated drops in a gas can be driven into rotation by altering their surrounding convective environment. When these drops are placed in an acoustic resonant chamber, the symmetry characteristics of the steady streaming flows in the vicinity of the drops determine the rotational motion of the freely suspended fluid particles. Using ultrasonic standing waves around 22 kHz and millimeter-size electrostatically levitated drops, we have investigated the correlation between the convective flow characteristics and their rotational behavior. The results show that accurate control of the drop rotation axis and rate can be obtained by carefully modifying the symmetry characteristics of the chamber, and that the dominant mechanism for rotation drive is the drag exerted by the air flow over the drop surface. In addition, we found that the rotational acceleration depends on the drop viscosity, suggesting that this torque is initially strongly influenced by differential flows within the drop itself. [Work sponsored by NASA].

  5. Solid lipid particles in lipid films to control diffusive release of 2-heptanone

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Controlled-release formulations of bioactive agents are gaining increasing interest for effective pest control. Volatile 2-heptanone is a bioactive agent that has shown potential to be used as a pesticide. The aim of this study was to investigate the kinetics of 2-heptanone release, which had been i...

  6. Fuel cell with internal flow control

    DOEpatents

    Haltiner, Jr., Karl J.; Venkiteswaran, Arun

    2012-06-12

    A fuel cell stack is provided with a plurality of fuel cell cassettes where each fuel cell cassette has a fuel cell with an anode and cathode. The fuel cell stack includes an anode supply chimney for supplying fuel to the anode of each fuel cell cassette, an anode return chimney for removing anode exhaust from the anode of each fuel cell cassette, a cathode supply chimney for supplying oxidant to the cathode of each fuel cell cassette, and a cathode return chimney for removing cathode exhaust from the cathode of each fuel cell cassette. A first fuel cell cassette includes a flow control member disposed between the anode supply chimney and the anode return chimney or between the cathode supply chimney and the cathode return chimney such that the flow control member provides a flow restriction different from at least one other fuel cell cassettes.

  7. Phosphatidylthreonine and Lipid-Mediated Control of Parasite Virulence

    PubMed Central

    Arroyo-Olarte, Ruben D.; Brouwers, Jos F.; Kuchipudi, Arunakar; Helms, J. Bernd; Biswas, Aindrila; Dunay, Ildiko R.; Lucius, Richard; Gupta, Nishith

    2015-01-01

    The major membrane phospholipid classes, described thus far, include phosphatidylcholine (PtdCho), phosphatidylethanolamine (PtdEtn), phosphatidylserine (PtdSer), and phosphatidylinositol (PtdIns). Here, we demonstrate the natural occurrence and genetic origin of an exclusive and rather abundant lipid, phosphatidylthreonine (PtdThr), in a common eukaryotic model parasite, Toxoplasma gondii. The parasite expresses a novel enzyme PtdThr synthase (TgPTS) to produce this lipid in its endoplasmic reticulum. Genetic disruption of TgPTS abrogates de novo synthesis of PtdThr and impairs the lytic cycle and virulence of T. gondii. The observed phenotype is caused by a reduced gliding motility, which blights the parasite egress and ensuing host cell invasion. Notably, the PTS mutant can prevent acute as well as yet-incurable chronic toxoplasmosis in a mouse model, which endorses its potential clinical utility as a metabolically attenuated vaccine. Together, the work also illustrates the functional speciation of two evolutionarily related membrane phospholipids, i.e., PtdThr and PtdSer. PMID:26565995

  8. Controlling a wide range of flow rates

    NASA Technical Reports Server (NTRS)

    Perkins, G. S.

    1979-01-01

    Servo-operated valve and two flowmeters allow accurate control over 1,900:1 flow-rate range. It was developed as part of laboratory instrument for measuring properties of confined fluids under conditions analogous to those encountered in deep drilling operations.

  9. Using fluorescence-activated flow cytometry to determine reactive oxygen species formation and membrane lipid peroxidation in viable boar spermatozoa

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fluorescence-activated flow cytometry analyses were developed for determination of reactive oxygen species (ROS) formation and membrane lipid peroxidation in live spermatozoa loaded with, respectively, hydroethidine (HE) or the lipophilic probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-d...

  10. The development of flow-through bio-catalyst microreactors from silica micro structured fibers for lipid transformations.

    PubMed

    Anuar, Sabiqah Tuan; Villegas, Carla; Mugo, Samuel M; Curtis, Jonathan M

    2011-06-01

    This study demonstrates the utility of a flow-through enzyme immobilized silica microreactor for lipid transformations. A silica micro structured fiber (MSF) consisting of 168 channels of internal diameter 4-5 μm provided a large surface area for the covalent immobilization of Candida antartica lipase. The specific activity of the immobilized lipase was determined by hydrolysis of p-nitrophenyl butyrate and calculated to be 0.81 U/mg. The catalytic performance of the lipase microreactor was demonstrated by the efficient ethanolysis of canola oil. The parameters affecting the performance of the MSF microreactor, including temperature and reaction flow rate, were investigated. Characterization of the lipid products exiting the microreactor was performed by non-aqueous reversed-phased liquid chromatography (NARP-LC) with evaporative light scattering detector (ELSD) and by comprehensive two-dimensional gas chromatography (GC x GC). Under optimized conditions of 1 μL/min flow rate of 5 mg/mL trioleoylglycerol (TO) in ethanol and 50 °C reaction temperature, 2-monooleoylglycerol was the main product at > 90% reaction yield. The regioselectivity of the Candida antartica lipase immobilized MSF microreactor in the presence of ethanol was found to be comparable to that obtained under conventional conditions. The ability of these reusable flow-through microreactors to regioselectively form monoacylglycerides in high yield from triacylglycerides demonstrate their potential use in small-scale lipid transformations or analytical lipids profiling. PMID:21318469

  11. Active flow control of subsonic flow in an adverse pressure gradient using synthetic jets and passive micro flow control devices

    NASA Astrophysics Data System (ADS)

    Denn, Michael E.

    Several recent studies have shown the advantages of active and/or passive flow control devices for boundary layer flow modification. Many current and future proposed air vehicles have very short or offset diffusers in order to save vehicle weight and create more optimal vehicle/engine integration. Such short coupled diffusers generally result in boundary layer separation and loss of pressure recovery which reduces engine performance and in some cases may cause engine stall. Deployment of flow control devices can alleviate this problem to a large extent; however, almost all active flow control devices have some energy penalty associated with their inclusion. One potential low penalty approach for enhancing the diffuser performance is to combine the passive flow control elements such as micro-ramps with active flow control devices such as synthetic jets to achieve higher control authority. The goal of this dissertation is twofold. The first objective is to assess the ability of CFD with URANS turbulence models to accurately capture the effects of the synthetic jets and micro-ramps on boundary layer flow. This is accomplished by performing numerical simulations replicating several experimental test cases conducted at Georgia Institute of Technology under the NASA funded Inlet Flow Control and Prediction Technologies Program, and comparing the simulation results with experimental data. The second objective is to run an expanded CFD matrix of numerical simulations by varying various geometric and other flow control parameters of micro-ramps and synthetic jets to determine how passive and active control devices interact with each other in increasing and/or decreasing the control authority and determine their influence on modification of boundary layer flow. The boundary layer shape factor is used as a figure of merit for determining the boundary layer flow quality/modification and its tendency towards separation. It is found by a large number of numerical experiments and

  12. Rotatable non-circular forebody flow controller

    NASA Technical Reports Server (NTRS)

    Moskovitz, Cary A. (Inventor)

    1991-01-01

    The invention is a rotatable, non-circular forebody flow controller. The apparatus comprises a small geometric device located at a nose of a forebody of an aircraft and a non-circular cross-sectional area that extends toward the apex of the aircraft. The device is symmetrical about a reference plane and preferably attaches to an axle which in turn attaches to a rotating motor. The motor rotates the device about an axis of rotation. Preferably, a control unit connected to an aircraft flight control computer signals to the rotating motor the proper rotational positioning of the geometric device.

  13. Nuclear engine flow reactivity shim control

    DOEpatents

    Walsh, J.M.

    1973-12-11

    A nuclear engine control system is provided which automatically compensates for reactor reactivity uncertainties at the start of life and reactivity losses due to core corrosion during the reactor life in gas-cooled reactors. The coolant gas flow is varied automatically by means of specially provided control apparatus so that the reactor control drums maintain a predetermined steady state position throughout the reactor life. This permits the reactor to be designed for a constant drum position and results in a desirable, relatively flat temperature profile across the core. (Official Gazette)

  14. Understanding the control of acyl flux through the lipid metabolic network of plant oil biosynthesis.

    PubMed

    Bates, Philip D

    2016-09-01

    Plant oil biosynthesis involves a complex metabolic network with multiple subcellular compartments, parallel pathways, cycles, and pathways that have a dual function to produce essential membrane lipids and triacylglycerol. Modern molecular biology techniques provide tools to alter plant oil compositions through bioengineering, however with few exceptions the final composition of triacylglycerol cannot be predicted. One reason for limited success in oilseed bioengineering is the inadequate understanding of how to control the flux of fatty acids through various fatty acid modification, and triacylglycerol assembly pathways of the lipid metabolic network. This review focuses on the mechanisms of acyl flux through the lipid metabolic network, and highlights where uncertainty resides in our understanding of seed oil biosynthesis. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner. PMID:27003249

  15. Plasma actuators for bluff body flow control

    NASA Astrophysics Data System (ADS)

    Kozlov, Alexey V.

    The aerodynamic plasma actuators have shown to be efficient flow control devices in various applications. In this study the results of flow control experiments utilizing single dielectric barrier discharge plasma actuators to control flow separation and unsteady vortex shedding from a circular cylinder in cross-flow are reported. This work is motivated by the need to reduce landing gear noise for commercial transport aircraft via an effective streamlining created by the actuators. The experiments are performed at Re D = 20,000...164,000. Circular cylinders in cross-flow are chosen for study since they represent a generic flow geometry that is similar in all essential aspects to a landing gear oleo or strut. The minimization of the unsteady flow separation from the models and associated large-scale wake vorticity by using actuators reduces the radiated aerodynamic noise. Using either steady or unsteady actuation at ReD = 25,000, Karman shedding is totally eliminated, turbulence levels in the wake decrease significantly and near-field sound pressure levels are reduced by 13.3 dB. Unsteady actuation at an excitation frequency of St D = 1 is found to be most effective. The unsteady actuation also has the advantage that total suppression of shedding is achieved for a duty cycle of only 25%. However, since unsteady actuation is associated with an unsteady body force and produces a tone at the actuation frequency, steady actuation is more suitable for noise control applications. Two actuation strategies are used at ReD = 82,000: spanwise and streamwise oriented actuators. Near field microphone measurements in an anechoic wind tunnel and detailed study of the near wake using LDA are presented in the study. Both spanwise and streamwise actuators give nearly the same noise reduction level of 11.2 dB and 14.2 dB, respectively, and similar changes in the wake velocity profiles. The contribution of the actuator induced noise is found to be small compared to the natural shedding

  16. Accurate, reliable control of process gases by mass flow controllers

    SciTech Connect

    Hardy, J.; McKnight, T.

    1997-02-01

    The thermal mass flow controller, or MFC, has become an instrument of choice for the monitoring and controlling of process gas flow throughout the materials processing industry. These MFCs are used on CVD processes, etching tools, and furnaces and, within the semiconductor industry, are used on 70% of the processing tools. Reliability and accuracy are major concerns for the users of the MFCs. Calibration and characterization technologies for the development and implementation of mass flow devices are described. A test facility is available to industry and universities to test and develop gas floe sensors and controllers and evaluate their performance related to environmental effects, reliability, reproducibility, and accuracy. Additional work has been conducted in the area of accuracy. A gravimetric calibrator was invented that allows flow sensors to be calibrated in corrosive, reactive gases to an accuracy of 0.3% of reading, at least an order of magnitude better than previously possible. Although MFCs are typically specified with accuracies of 1% of full scale, MFCs may often be implemented with unwarranted confidence due to the conventional use of surrogate gas factors. Surrogate gas factors are corrections applied to process flow indications when an MFC has been calibrated on a laboratory-safe surrogate gas, but is actually used on a toxic, or corrosive process gas. Previous studies have indicated that the use of these factors may cause process flow errors of typically 10%, but possibly as great as 40% of full scale. This paper will present possible sources of error in MFC process gas flow monitoring and control, and will present an overview of corrective measures which may be implemented with MFC use to significantly reduce these sources of error.

  17. MEMS applications in turbulence and flow control

    NASA Astrophysics Data System (ADS)

    Löfdahl, Lennart; Gad-el-Hak, Mohamed

    1999-02-01

    Manufacturing processes that can create extremely small machines have been developed in recent years. Microelectromechanical systems (MEMS) refer to devices that have characteristic length of less than 1 mm but more than 1 μm, that combine electrical and mechanical components and that are fabricated using integrated circuit batch-processing techniques. Electrostatic, magnetic, pneumatic and thermal actuators, motors, valves, gears and tweezers of less than 100 μm size have been fabricated. These have been used as sensors for pressure, temperature, mass flow, velocity and sound, as actuators for linear and angular motions, and as simple components for complex systems such as micro-heat-engines and micro-heat-pumps. In this paper, we focus on the use of microelectromechanical systems for the diagnosis and control of turbulent shear flows. We survey the status and outlook of microsensors and microactuators as used for those particular applications, and compare the minute devices to their larger cousins. Microsensors can resolve all relevant scales even in high-Reynolds-number turbulent flows. Arrays of microsensors and microactuators make it feasible, for the first time, to achieve effective reactive control targeted toward specific small-scale coherent structures in turbulent wall-bounded flows.

  18. Control of interfacial instabilities using flow geometry

    NASA Astrophysics Data System (ADS)

    Al-Housseiny, Talal T.; Tsai, Peichun A.; Stone, Howard A.

    2012-10-01

    The displacement of one fluid by another is one of the most common processes involving interfacial instabilities. It is universally accepted that, in a uniform medium, flow displacement is unstable when a low-viscosity fluid invades a fluid of higher viscosity: the classical viscous fingering instability. Consequently, once fluid properties are specified, opportunities for control become very limited. However, real systems where displacement instabilities occur, such as porous structures, lung airways and printing devices, are rarely uniform. We find that the simplest heterogeneity--a gradient in the flow passage--can lead to fundamentally different displacement behaviours. We use this finding to either inhibit or trigger an instability and, hence, to devise a strategy to manipulate instabilities in fluid-fluid systems. The control setting we identify has a wide spectrum of applications ranging from small-scale technologies such as microfluidics to large-scale operations such as enhanced oil recovery.

  19. Controlling solid lipid nanoparticle adhesion by polyelectrolyte multilayer surface modifications.

    PubMed

    Finke, Jan Henrik; Schmolke, Hannah; Klages, C-P; Müller-Goymann, Christel C

    2013-06-01

    This study addresses the tunability of polyelectrolyte multilayers (PEM) toward adsorption of solid lipid nanoparticles (SLN). In SLN production for pharmaceutical applications, repellence from production equipment is desired while targeted adsorption is necessary for the functionalization of surfaces. SLN containing triglyceride/phospholipid or wax matrices were exposed to different PEM (consisting of poly(allylamine hydrochloride) (PAH), poly(diallyldimethylammonium chloride), and poly(acrylic acid)). PEM varied regarding layer architecture and surface properties by means of deposition pH, top layer variation, PEGylation with poly(acrylic acid)-graft-poly(ethylene glycol) copolymer, and thermal crosslinking. FTIR-ATR and SEM revealed SLN adhesion depending on PEM composition. Particle adsorption was tunable toward attraction as well as repellence: PEGylated PEM displayed lowest adsorption while PEM capped with PAH provided the strongest attraction of particles. Examinations at elevated temperatures resembled production conditions of SLN where these are processed as emulsions. Crystalline triglyceride SLN displayed high anisometry and, consequently, a large specific surface area. These platelets were more adherend than spherical droplets from the same formulation as an emulsion. Wax-based nanoparticles showed spherical shape, both in crystalline and molten state. However, adsorption was fostered as the fluidity of the disperse phase increased upon melting. Additionally, coalescence of adsorbed droplets took place, further increasing adsorption. PMID:23591009

  20. Shallow flow vortex formation and control

    NASA Astrophysics Data System (ADS)

    Fu, Haojun

    Vortical structures in shallow flow past a vertical cylinder are addressed in this investigation. A cinema technique of digital particle image velocimetry (DPIV) provided quantitative representations of the wholefield flow patterns in both instantaneous and averaged forms. Techniques for passive and active control of these vortices, and their influence on the loading of the bed, were explored. In a fully-developed, laminar shallow flow, the unstable structure in the near-wake of the cylinder correlates with the horseshoe (necklace) vortex system about the upstream surface of the cylinder. A coherent varicose mode of vortex formation is observed in the near-wake, even though the classical large-scale vortex shedding is suppressed due to bed friction effects. It is also demonstrated that when the near-wake is stable at a sufficiently low value of Reynolds number, applications of external perturbations lead to destabilization of the wake. Classes of small-scale three-dimensional structures arise in a fully-turbulent shallow flow past a surface-piercing cylinder. A prevalent feature is an upward moving jet-like flow from the bed surface, through the center of the developing quasi-two-dimensional primary vortex, at a location in the very near-wake of the cylinder. Passive control via base-bleed through a narrow streamwise slot leads to substantially delay/attenuation of vortex formation in the near-wake. The large-scale near-wake structure is recoverable through combined positive-active control, in the form of rotational perturbations in the presence of small magnitude base bleed. These alterations of the near-wake structure occur in conjunction with modifications of the streamline topology and Reynolds stress at the bed, as well as the shallow approach flow. Active control via rotational perturbations of the cylinder at the most unstable shear-layer frequency promotes well-defined vortical structures in the separating shearlayer, which contribute to the earlier

  1. Microfluidic assembly of multistage porous silicon–lipid vesicles for controlled drug release

    PubMed Central

    Herranz-Blanco, Bárbara; Arriaga, Laura R.; Mäkilä, Ermei; Correia, Alexandra; Shrestha, Neha; Mirza, Sabiruddin; Weitz, David A.; Salonen, Jarno; Hirvonen, Jouni; Santos, Hélder A.

    2014-01-01

    A reliable microfluidic platform for the generation of stable and monodisperse multistage drug delivery systems is reported. A glass-capillary flow-focusing droplet generation device was used to encapsulate thermally hydrocarbonized porous silicon (PSi) microparticles into the aqueous cores of double emulsion drops, yielding the formation of a multistage PSi-lipid vesicle. This composite system enables a large loading capacity for hydrophobic drugs. PMID:24469311

  2. Electromechanically Actuated Valve for Controlling Flow Rate

    NASA Technical Reports Server (NTRS)

    Patterson, Paul

    2007-01-01

    A proposed valve for controlling the rate of flow of a fluid would include an electric-motor-driven ball-screw mechanism for adjusting the seating element of the valve to any position between fully closed and fully open. The motor would be of a type that can be electronically controlled to rotate to a specified angular position and to rotate at a specified rate, and the ball screw would enable accurate linear positioning of the seating element as a function of angular position of the motor. Hence, the proposed valve would enable fine electronic control of the rate of flow and the rate of change of flow. The uniqueness of this valve lies in a high degree of integration of the actuation mechanism with the flow-control components into a single, relatively compact unit. A notable feature of this integration is that in addition to being a major part of the actuation mechanism, the ball screw would also be a flow-control component: the ball screw would be hollow so as to contain part of the main flow passage, and one end of the ball screw would be the main seating valve element. The relationships among the components of the valve are best understood by reference to the figure, which presents meridional cross sections of the valve in the fully closed and fully open positions. The motor would be supported by a bracket bolted to the valve body. By means of gears or pulleys and a timing belt, motor drive would be transmitted to a sleeve that would rotate on bearings in the valve body. A ball nut inside the sleeve would be made to rotate with the sleeve by use of a key. The ball screw would pass through and engage the ball nut. A key would prevent rotation of the ball screw in the valve body while allowing the ball screw to translate axially when driven by the ball nut. The outer surface of the ball screw would be threaded only in a mid-length region: the end regions of the outer surface of the ball screw would be polished so that they could act as dynamic sealing surfaces

  3. Flow-controlled magnetic particle manipulation

    DOEpatents

    Grate, Jay W [West Richland, WA; Bruckner-Lea, Cynthia J [Richland, WA; Holman, David A [Las Vegas, NV

    2011-02-22

    Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection/resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.

  4. Comparison of drug release from liquid crystalline monoolein dispersions and solid lipid nanoparticles using a flow cytometric technique

    PubMed Central

    Dawoud, Mohamed Z.; Nasr, Mohamed

    2016-01-01

    Colloidal lipid particles such as solid lipid nanoparticles and liquid crystalline nanoparticles have great opportunities as drug carriers especially for lipophilic drugs intended for intravenous administration. In order to evaluate drug release from these nanoparticles and determine their behavior after administration, emulsion droplets were used as a lipophilic compartment to which the transfer of a model drug was measured. The detection of the model drug transferred from monoolein cubic particles and trimyristin solid lipid nanoparticles into emulsion droplets was performed using a flow cytometric technique. A higher rate and amount of porphyrin transfer from the solid lipid nanoparticles compared to the monoolein cubic particles was observed. This difference might be attributed to the formation of a highly ordered particle which leads to the expulsion of drug to the surface of the crystalline particle. Furthermore, the sponge-like structure of the monoolein cubic particles decreases the rate and amount of drug transferred. In conclusion, the flow cytometric technique is a suitable technique to study drug transfer from these carriers to large lipophilic acceptors. Monoolein cubic particles with their unique structure can be used successfully as a drug carrier with slow drug release compared with trimyristin nanoparticles. PMID:27006901

  5. Size fractionation and size characterization of nanoemulsions of lipid droplets and large unilamellar lipid vesicles by asymmetric-flow field-flow fractionation/multi-angle light scattering and dynamic light scattering.

    PubMed

    Vezočnik, Valerija; Rebolj, Katja; Sitar, Simona; Ota, Katja; Tušek-Žnidarič, Magda; Štrus, Jasna; Sepčić, Kristina; Pahovnik, David; Maček, Peter; Žagar, Ema

    2015-10-30

    Asymmetric-flow field-flow fractionation technique coupled to a multi-angle light-scattering detector (AF4-MALS) was used together with dynamic light-scattering (DLS) in batch mode and transmission electron microscopy (TEM) to study the size characteristics of the trioleoylglycerol lipid droplets covered by a monolayer of sphingomyelin and cholesterol, in water phase. These lipid droplet nanoemulsions (LD) were formed by ultrasonication. In parallel, the size characteristics of large unilamellar lipid vesicles (LUV) prepared by extrusion and composed of sphingomyelin and cholesterol were determined. LD and LUV were prepared at two different molar ratios (1/1, 4/1) of sphingomyelin and cholesterol. In AF4-MALS, various cross-flow conditions and mobile phase compositions were tested to optimize the separation of LD or LUV particles. The particle radii, R, as well as the root-mean-square radii, Rrms, of LD and LUV were determined by AF4-MALS, whereas the hydrodynamic radii, Rh, were obtained by DLS. TEM visualization revealed round shape particles of LD and LUV. PMID:26409772

  6. Active Flow Control Strategies Using Surface Pressure Measurements

    NASA Technical Reports Server (NTRS)

    Kumar, Vikas; Alvi, Farrukh S.

    2010-01-01

    Evaluate the efficacy of Microjets Can we eliminate/minimize flow separation? Is the flow unsteadiness reduced? Guidelines for an active control Search for an appropriate sensor. Examine for means to develop a flow model for identifying the state of flow over the surface Guidelines toward future development of a Simple and Robust control methodology

  7. Active Flow Control Activities at NASA Langley

    NASA Technical Reports Server (NTRS)

    Anders, Scott G.; Sellers, William L., III; Washburn, Anthony E.

    2004-01-01

    NASA Langley continues to aggressively investigate the potential advantages of active flow control over more traditional aerodynamic techniques. This paper provides an update to a previous paper and describes both the progress in the various research areas and the significant changes in the NASA research programs. The goals of the topics presented are focused on advancing the state of knowledge and understanding of controllable fundamental mechanisms in fluids as well as to address engineering challenges. An organizational view of current research activities at NASA Langley in active flow control as supported by several projects is presented. On-center research as well as NASA Langley funded contracts and grants are discussed at a relatively high level. The products of this research are to be demonstrated either in bench-top experiments, wind-tunnel investigations, or in flight as part of the fundamental NASA R&D program and then transferred to more applied research programs within NASA, DOD, and U.S. industry.

  8. Thermoregulatory control of finger blood flow

    NASA Technical Reports Server (NTRS)

    Wenger, C. B.; Roberts, M. F.; Nadel, E. R.; Stolwijk, J. A. J.

    1975-01-01

    In the present experiment, exercise was used to vary internal temperature and ambient air heat control was used to vary skin temperature. Finger temperature was fixed at about 35.7 C. Esophageal temperature was measured with a thermocouple at the level of the left atrium, and mean skin temperature was calculated from a weighted mean of thermocouple temperatures at different skin sites. Finger blood flow was measured by electrocapacitance plethysmography. An equation in these quantities is given which accounts for the data garnered.

  9. Design Considerations for Laminar Flow Control Aircraft

    NASA Technical Reports Server (NTRS)

    Sturgeon, R. F.; Bennett, J. A.

    1976-01-01

    A study was conducted to investigate major design considerations involved in the application of laminar flow control to the wings and empennage of long range subsonic transport aircraft compatible with initial operation in 1985. For commercial transports with a design mission range of 10,186 km (5500 n mil) and a payload of 200 passengers, parametric configuration analyses were conducted to evaluate the effect of aircraft performance, operational, and geometric parameters on fuel efficiency. Study results indicate that major design goals for aircraft optimization include maximization of aspect ratio and wing loading and minimization of wing sweep consistent with wing volume and airport performance requirements.

  10. Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae

    PubMed Central

    Cho, Myung Kwon; Shin, Hwa Sung

    2016-01-01

    Microalgae lipids are a promising energy source, but current biochemical methods of lipid-inductions such as nitrogen deprivation have low process robustness and controllability. Recently, use of mechanotransduction based membrane distortion by applying compression stress in a 2D-microsystem was suggested as a way to overcome these limitations of biochemical induction. However, reproduction in large numbers of cells without cell death has been difficult to overcome because compression for direct membrane distortion reduces culture volume and leads to cell death due to nutrient deprivation. In this study, a mechanotransduction-induced lipid production (MDLP) system that redirects elastic microbeads to induce membrane distortion of microalgae with alleviating cell death was developed. This system resulted in accumulation of lipid in as little as 4 hr. Once compressed, porous microbeads absorb media and swell simultaneously while homogeneously inducing compression stress of microalgae. The absorbed media within beads could be supplied to adjacent cells and could minimize cell death from nutrient deficiency. All mechanotransduction was confirmed by measuring upregulation of calcium influx and Mat3 genes. The microbeads ensured robustness and controllability in repeated compression/de-compression processes. Overall, the MDLP system has potential for use as a fundamental biodiesel process that requires robustness and controllability. PMID:27609701

  11. Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae.

    PubMed

    Cho, Myung Kwon; Shin, Hwa Sung

    2016-01-01

    Microalgae lipids are a promising energy source, but current biochemical methods of lipid-inductions such as nitrogen deprivation have low process robustness and controllability. Recently, use of mechanotransduction based membrane distortion by applying compression stress in a 2D-microsystem was suggested as a way to overcome these limitations of biochemical induction. However, reproduction in large numbers of cells without cell death has been difficult to overcome because compression for direct membrane distortion reduces culture volume and leads to cell death due to nutrient deprivation. In this study, a mechanotransduction-induced lipid production (MDLP) system that redirects elastic microbeads to induce membrane distortion of microalgae with alleviating cell death was developed. This system resulted in accumulation of lipid in as little as 4 hr. Once compressed, porous microbeads absorb media and swell simultaneously while homogeneously inducing compression stress of microalgae. The absorbed media within beads could be supplied to adjacent cells and could minimize cell death from nutrient deficiency. All mechanotransduction was confirmed by measuring upregulation of calcium influx and Mat3 genes. The microbeads ensured robustness and controllability in repeated compression/de-compression processes. Overall, the MDLP system has potential for use as a fundamental biodiesel process that requires robustness and controllability. PMID:27609701

  12. Distributed Power Flow Control: Distributed Power Flow Control using Smart Wires for Energy Routing

    SciTech Connect

    2012-04-24

    GENI Project: Smart Wire Grid is developing a solution for controlling power flow within the electric grid to better manage unused and overall transmission capacity. The 300,000 miles of high-voltage transmission line in the U.S. today are congested and inefficient, with only around 50% of all transmission capacity utilized at any given time. Increased consumer demand should be met in part with more efficient and an economical power flow. Smart Wire Grid’s devices clamp onto existing transmission lines and control the flow of power within—much like how internet routers help allocate bandwidth throughout the web. Smart wires could support greater use of renewable energy by providing more consistent control over how that energy is routed within the grid on a real-time basis. This would lessen the concerns surrounding the grid’s inability to effectively store intermittent energy from renewables for later use.

  13. Measurement of Lipid Accumulation in Chlorella vulgaris via Flow Cytometry and Liquid-State ¹H NMR Spectroscopy for Development of an NMR-Traceable Flow Cytometry Protocol

    PubMed Central

    Bono Jr., Michael S.; Garcia, Ravi D.; Sri-Jayantha, Dylan V.; Ahner, Beth A.; Kirby, Brian J.

    2015-01-01

    In this study, we cultured Chlorella vulgaris cells with a range of lipid contents, induced via nitrogen starvation, and characterized them via flow cytometry, with BODIPY 505/515 as a fluorescent lipid label, and liquid-state 1H NMR spectroscopy. In doing so, we demonstrate the utility of calibrating flow cytometric measurements of algal lipid content using triacylglyceride (TAG, also known as triacylglycerol or triglyceride) content per cell as measured via quantitative 1H NMR. Ensemble-averaged fluorescence of BODIPY-labeled cells was highly correlated with average TAG content per cell measured by bulk NMR, with a linear regression yielding a linear fit with r2 = 0.9974. This correlation compares favorably to previous calibrations of flow cytometry protocols to lipid content measured via extraction, and calibration by NMR avoids the time and complexity that is generally required for lipid quantitation via extraction. Flow cytometry calibrated to a direct measurement of TAG content can be used to investigate the distribution of lipid contents for cells within a culture. Our flow cytometry measurements showed that Chlorella vulgaris cells subjected to nitrogen limitation exhibited higher mean lipid content but a wider distribution of lipid content that overlapped the relatively narrow distribution of lipid content for replete cells, suggesting that nitrogen limitation induces lipid accumulation in only a subset of cells. Calibration of flow cytometry protocols using direct in situ measurement of TAG content via NMR will facilitate rapid development of more precise flow cytometry protocols, enabling investigation of algal lipid accumulation for development of more productive algal biofuel feedstocks and cultivation protocols. PMID:26267664

  14. Control of Inward Rectifier K Channel Activity by Lipid Tethering of Cytoplasmic Domains

    PubMed Central

    Enkvetchakul, Decha; Jeliazkova, Iana; Bhattacharyya, Jaya; Nichols, Colin G.

    2007-01-01

    Interactions between nontransmembrane domains and the lipid membrane are proposed to modulate activity of many ion channels. In Kir channels, the so-called “slide-helix” is proposed to interact with the lipid headgroups and control channel gating. We examined this possibility directly in a cell-free system consisting of KirBac1.1 reconstituted into pure lipid vesicles. Cysteine substitution of positively charged slide-helix residues (R49C and K57C) leads to loss of channel activity that is rescued by in situ restoration of charge following modification by MTSET+ or MTSEA+, but not MTSES− or neutral MMTS. Strikingly, activity is also rescued by modification with long-chain alkyl-MTS reagents. Such reagents are expected to partition into, and hence tether the side chain to, the membrane. Systematic scanning reveals additional slide-helix residues that are activated or inhibited following alkyl-MTS modification. A pattern emerges whereby lipid tethering of the N terminus, or C terminus, of the slide-helix, respectively inhibits, or activates, channel activity. This study establishes a critical role of the slide-helix in Kir channel gating, and directly demonstrates that physical interaction of soluble domains with the membrane can control ion channel activity. PMID:17698595

  15. Identification of regulatory network hubs that control lipid metabolism in Chlamydomonas reinhardtii.

    PubMed

    Gargouri, Mahmoud; Park, Jeong-Jin; Holguin, F Omar; Kim, Min-Jeong; Wang, Hongxia; Deshpande, Rahul R; Shachar-Hill, Yair; Hicks, Leslie M; Gang, David R

    2015-08-01

    Microalgae-based biofuels are promising sources of alternative energy, but improvements throughout the production process are required to establish them as economically feasible. One of the most influential improvements would be a significant increase in lipid yields, which could be achieved by altering the regulation of lipid biosynthesis and accumulation. Chlamydomonas reinhardtii accumulates oil (triacylglycerols, TAG) in response to nitrogen (N) deprivation. Although a few important regulatory genes have been identified that are involved in controlling this process, a global understanding of the larger regulatory network has not been developed. In order to uncover this network in this species, a combined omics (transcriptomic, proteomic and metabolomic) analysis was applied to cells grown in a time course experiment after a shift from N-replete to N-depleted conditions. Changes in transcript and protein levels of 414 predicted transcription factors (TFs) and transcriptional regulators (TRs) were monitored relative to other genes. The TF and TR genes were thus classified by two separate measures: up-regulated versus down-regulated and early response versus late response relative to two phases of polar lipid synthesis (before and after TAG biosynthesis initiation). Lipidomic and primary metabolite profiling generated compound accumulation levels that were integrated with the transcript dataset and TF profiling to produce a transcriptional regulatory network. Evaluation of this proposed regulatory network led to the identification of several regulatory hubs that control many aspects of cellular metabolism, from N assimilation and metabolism, to central metabolism, photosynthesis and lipid metabolism. PMID:26022256

  16. Identification of regulatory network hubs that control lipid metabolism in Chlamydomonas reinhardtii

    PubMed Central

    Gargouri, Mahmoud; Park, Jeong-Jin; Holguin, F. Omar; Kim, Min-Jeong; Wang, Hongxia; Deshpande, Rahul R.; Shachar-Hill, Yair; Hicks, Leslie M.; Gang, David R.

    2015-01-01

    Microalgae-based biofuels are promising sources of alternative energy, but improvements throughout the production process are required to establish them as economically feasible. One of the most influential improvements would be a significant increase in lipid yields, which could be achieved by altering the regulation of lipid biosynthesis and accumulation. Chlamydomonas reinhardtii accumulates oil (triacylglycerols, TAG) in response to nitrogen (N) deprivation. Although a few important regulatory genes have been identified that are involved in controlling this process, a global understanding of the larger regulatory network has not been developed. In order to uncover this network in this species, a combined omics (transcriptomic, proteomic and metabolomic) analysis was applied to cells grown in a time course experiment after a shift from N-replete to N-depleted conditions. Changes in transcript and protein levels of 414 predicted transcription factors (TFs) and transcriptional regulators (TRs) were monitored relative to other genes. The TF and TR genes were thus classified by two separate measures: up-regulated versus down-regulated and early response versus late response relative to two phases of polar lipid synthesis (before and after TAG biosynthesis initiation). Lipidomic and primary metabolite profiling generated compound accumulation levels that were integrated with the transcript dataset and TF profiling to produce a transcriptional regulatory network. Evaluation of this proposed regulatory network led to the identification of several regulatory hubs that control many aspects of cellular metabolism, from N assimilation and metabolism, to central metabolism, photosynthesis and lipid metabolism. PMID:26022256

  17. Physical and chemical gels of lipid nanoparticles for controlled delivery of lipophilic drugs and proteins

    NASA Astrophysics Data System (ADS)

    Couffin, Anne-Claude; Delmas, Thomas; Thomann, Jean-Sébastien; Cheibani, Ismail; Bayma, Eric; Heinrich, Emilie; Escudé, Marie; Courant, Thomas; Hoang, Antoine; Auzély, Rachel; Texier, Isabelle

    2013-05-01

    The controlled delivery of drugs and biologicals (proteins, antibodies, DNA and derivatives) is a growing need to take the full benefit of new therapeutic strategies. However these new molecules or biomolecules display solubility issues, or high degradation rates once injected. Therefore, both suitable delivery materials for their encapsulation and protection from the surrounding environment, and smart delivery devices (such as micro-needles or implanted pumps) are necessary to achieve controlled delivery of these precious therapeutic agents. We have developed bio-inspired gel materials, based on lipid nanoparticles which act as reservoirs for lipophilic drugs. The lipid nanoparticles, termed lipidots™, are biocompatible, colloidally stable, non-immunogenic, and obtained from a cheap and simple solvent-free process. The particles can be assembled to form physical or chemical gels, with tunable rheological properties. Physico-chemical studies have been carried out to determine the limits of the stability domains for colloidal and gel formulations (choice of surfactants for nanoparticle surface, and composition ratios of lipids, surfactants and co-surfactants). In particular, it is demonstrated that lipid nanoparticles keep their integrity in the gels. Gels of lipidots™ could therefore constitute biocompatible materials for the efficient encapsulation and tuned delivery of lipophilic drugs and biomolecules.

  18. Brain lipid sensing and the neural control of energy balance.

    PubMed

    Magnan, Christophe; Levin, Barry E; Luquet, Serge

    2015-12-15

    Fatty acid (FA) -sensitive neurons are present in the brain, especially the hypothalamus, and play a key role in the neural control of energy and glucose homeostasis including feeding behavior, secretion insulin and action. Subpopulations of neurons in the arcuate and ventromedial hypothalamic nuclei are selectively either activated or inhibited by FA. Molecular effectors of these FA effects include ion channels such as chloride, potassium or calcium. In addition, at least half of the responses in the hypothalamic ventromedial FA neurons are mediated through interaction with the FA translocator/receptor, FAT/CD36, that does not require metabolism to activate intracellular signaling downstream. Recently, an important role of lipoprotein lipase in FA detection has also been demonstrated not only in the hypothalamus, but also in the hippocampus and striatum. Finally, FA could overload energy homeostasis via increased hypothalamic ceramide synthesis which could, in turn, contribute to the pathogenesis of diabetes of obesity and/or type 2 in predisposed individuals by disrupting the endocrine signaling pathways of insulin and/or leptin. PMID:26415589

  19. Effect of Selenium Supplementation on Glycemic Control and Lipid Profiles in Patients with Diabetic Nephropathy.

    PubMed

    Bahmani, Fereshteh; Kia, Mahsa; Soleimani, Alireza; Asemi, Zatollah; Esmaillzadeh, Ahmad

    2016-08-01

    To our knowledge, data on the effects of selenium supplementation on glycemic control and lipid concentrations in patients with diabetic nephropathy (DN) are scarce. The current study was done to determine the effects of selenium supplementation on glycemic control and lipid concentrations in patients with DN. This was a randomized double-blind placebo-controlled clinical trial in which 60 patients with DN were randomly allocated into two groups to receive either 200 μg of selenium supplements (n = 30) or placebo (n = 30) daily for 12 weeks. Blood sampling was performed for the quantification of glycemic indicators and lipid profiles at the onset of the study and after 12 weeks of intervention. Selenium supplementation for 12 weeks resulted in a significant decrease in serum insulin levels (P = 0.01), homeostasis model of assessment-estimated insulin resistance (HOMA-IR) (P = 0.02), homeostasis model of assessment-estimated B cell function (HOMA-B) (P = 0.009) and a significant rise in plasma glutathione peroxidase (GPx) (P = 0.001) compared with the placebo. Taking selenium supplements had no significant effects on fasting plasma glucose (FPG), quantitative insulin sensitivity check index (QUICKI) and lipid profiles compared with the placebo. Overall, our study demonstrated that selenium supplementation for 12 weeks among patients with DN had beneficial effects on plasma GPx, serum insulin levels, HOMA-IR, and HOMA-B, while it did not affect FPG, QUICKI, and lipid profiles. PMID:26686847

  20. Spheronization of solid lipid extrudates: A novel approach on controlling critical process parameters.

    PubMed

    Petrovick, Gustavo Freire; Pein, Miriam; Thommes, Markus; Breitkreutz, Jörg

    2015-05-01

    Solid lipids are non-toxic excipients, which are known to potentially enhance delivery and bioavailability of poorly water-soluble drugs and moreover to mask unpleasant tasting drugs. Multiple unit matrix dosage forms based on solid lipids, such as lipid pellets, can be obtained by solvent-free cold extrusion and spheronization. This method presents advantages in the processing of sensitive substances, such as low process temperatures, the absence of solvents and a drying step. However, the material temperature during the spheronization showed to be critical so far. The process leads to increased material temperatures, causing particle agglomeration and discontinuity of the spheronization. In the present study, extrudates of 0.5mm in diameter containing metformin hydrochloride, and either semisynthetic hard fat (Witocan® 42/44) or different ternary mixtures based on hard fat, glyceryl trimyristate, and glyceryl distearate, were spheronized. By applying common process parameters, particle agglomeration or material stickiness on equipment walls was observed in preliminary experiments after 2-6min, depending on the lipid composition. Therefore, an innovative instrumental setup to control the spheronization process was developed utilizing an infrared light source, which was positioned over the particle bed. The new approach enabled a spheronization process that reached the desired spheronization temperature after 2-3min and neither particle agglomeration nor material adherence occurred even after longer process times. The different formulations, even those based on high amount of solid lipids, were successfully spheronized over 15min, resulting in small diameter lipid pellets with smooth surface and aspect ratios below 1.3. PMID:25681745

  1. Controlling the localization of polymer-functionalized nanoparticles in mixed lipid/polymer membranes.

    PubMed

    Olubummo, Adekunle; Schulz, Matthias; Lechner, Bob-Dan; Scholtysek, Peggy; Bacia, Kirsten; Blume, Alfred; Kressler, Jörg; Binder, Wolfgang H

    2012-10-23

    Surface hydrophobicity plays a significant role in controlling the interactions between nanoparticles and lipid membranes. In principle, a nanoparticle can be encapsulated into a liposome, either being incorporated into the hydrophobic bilayer interior or trapped within the aqueous vesicle core. In this paper, we demonstrate the preparation and characterization of polymer-functionalized CdSe NPs, tuning their interaction with mixed lipid/polymer membranes from 1,2-dipalmitoyl-sn-glycero-3-phophocholine and PIB(87)-b-PEO(17) block copolymer by varying their surface hydrophobicity. It is observed that hydrophobic PIB-modified CdSe NPs can be selectively located within polymer domains in a mixed lipid/polymer monolayer at the air/water interface, changing their typical domain morphologies, while amphiphilic PIB-PEO-modified CdSe NPs showed no specific localization in phase-separated lipid/polymer films. In addition, hydrophilic water-soluble CdSe NPs can readily adsorb onto spread monolayers, showing a larger effect on the molecule packing at the air/water interface in the case of pure lipid films compared to mixed monolayers. Furthermore, the incorporation of PIB-modified CdSe NPs into hybrid lipid/polymer GUVs is demonstrated with respect to the prevailing phase state of the hybrid membrane. Monitoring fluorescent-labeled PIB-CdSe NPs embedded into phase-separated vesicles, it is demonstrated that they are enriched in one specific phase, thus probing their selective incorporation into the hydrophobic portion of PIB(87)-b-PEO(17) BCP-rich domains. Thus, the formation of biocompatible hybrid GUVs with selectively incorporated nanoparticles opens a new perspective for subtle engineering of membranes together with their (nano-) phase structure serving as a model system in designing functional nanomaterials for effective nanomedicine or drug delivery. PMID:22950802

  2. Localized flow control with energy deposition

    NASA Astrophysics Data System (ADS)

    Adelgren, Russell Gene

    A series of experiments with energy deposition via laser-induced optical breakdown of air, i.e., a laser spark, have been performed. These experiments have demonstrated the possibility of using a laser spark for supersonic flow control. In the first of these experiments, Rayleigh scattering flow visualization was taken for energy deposition into quiescent air. A time sequence of images showed the post breakdown fluid motion created by the laser spark for different laser energy levels. Blast wave radius and wave speed measurements were made and correlated to five different laser energy deposition levels. Laser energy was deposited upstream of a sphere in Mach 3.45 flow. The energy was deposited one sphere diameter and 0.6 diameters upstream of the front of the sphere. The frontal surface pressure on the sphere was recorded as the laser spark perturbed region interacted with the flow about the sphere. Tests for three different energy levels and two different incident laser beam diameters were completed. It has been demonstrated that the peak surface pressure associated with the Edney IV interaction can be momentarily reduced by 30% by the interaction with the thermal spot created by the laser spark. The effects of laser energy deposition on another shock interaction phenomena were studied. Laser energy deposition was used to modify the shock structure formed by symmetric wedges at Mach 3.45 within the dual solution domain. It was demonstrated experimentally that the Mach reflection could be reduced by 80% momentarily. The numerical simulations show a transition from the stable Mach reflection to a stable regular reflection. Two energy deposition methods (electric arcing and laser energy deposition) were used to force and control compressible mixing layers of axisymmetric jets. The energy deposition forcing methods have been experimentally investigated with the schlieren technique, particle image velocimetry, Mie scattering, and static pressure probe diagnostic

  3. Ca2+-regulated cyclic electron flow supplies ATP for nitrogen starvation-induced lipid biosynthesis in green alga

    PubMed Central

    Chen, Hui; Hu, Jinlu; Qiao, Yaqin; Chen, Weixian; Rong, Junfeng; Zhang, Yunming; He, Chenliu; Wang, Qiang

    2015-01-01

    We previously showed that both the linear photosynthetic electron transportation rate and the respiration rate dropped significantly during N starvation-induced neutral lipid accumulation in an oil-producing microalga, Chlorella sorokiniana, and proposed a possible role for cyclic electron flow (CEF) in ATP supply. In this study, we further exploited this hypothesis in both Chlorella sorokiniana C3 and the model green alga Chlamydomonas. We found that both the rate of CEF around photosystem I and the activity of thylakoid membrane-located ATP synthetase increased significantly during N starvation to drive ATP production. Furthermore, we demonstrated that the Chlamydomonas mutant pgrl1, which is deficient in PGRL1-mediated CEF, accumulated less neutral lipids and had reduced rates of CEF under N starvation. Further analysis revealed that Ca2+ signaling regulates N starvation-induced neutral lipid biosynthesis in Chlamydomonas by increasing calmodulin activity and boosting the expression of the calcium sensor protein that regulates Pgrl1-mediated CEF. Thus, Ca2+-regulated CEF supplies ATP for N starvation-induced lipid biosynthesis in green alga. The increased CEF may re-equilibrate the ATP/NADPH balance and recycle excess light energy in photosystems to prevent photooxidative damage, suggesting Ca2+-regulated CEF also played a key role in protecting and sustaining photosystems. PMID:26450399

  4. Using fluorescence-activated flow cytometry to determine reactive oxygen species formation and membrane lipid peroxidation in viable boar spermatozoa.

    PubMed

    Guthrie, H David; Welch, Glenn R

    2010-01-01

    Fluorescence-activated flow cytometry analyses were developed for determination of reactive oxygen species (ROS) formation and membrane lipid peroxidation in live spermatozoa loaded with, respectively, hydroethidine (HE) or the lipophilic probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, C(11)BODIPY(581/591) (BODIPY). ROS was detected by red fluorescence emission from oxidization of HE and membrane lipid peroxidation was detected by green fluorescence emission from oxidation of BODIPY in individual live sperm. Of the reactive oxygen species generators tested, BODIPY oxidation was specific for FeSo4/ascorbate (FeAc), because menadione and H(2)O(2) had little or no effect. The oxidization of hydroethidine to ethidium was specific for menadione and H(2)O(2); FeAc had no effect. The incidence of basal or spontaneous ROS formation and membrane lipid peroxidation were low in boar sperm (<1% of live sperm) in fresh semen or after low temperature storage; however the sperm were quite susceptible to treatment-induced ROS formation and membrane lipid peroxidation. PMID:20072917

  5. Ca(2+)-regulated cyclic electron flow supplies ATP for nitrogen starvation-induced lipid biosynthesis in green alga.

    PubMed

    Chen, Hui; Hu, Jinlu; Qiao, Yaqin; Chen, Weixian; Rong, Junfeng; Zhang, Yunming; He, Chenliu; Wang, Qiang

    2015-01-01

    We previously showed that both the linear photosynthetic electron transportation rate and the respiration rate dropped significantly during N starvation-induced neutral lipid accumulation in an oil-producing microalga, Chlorella sorokiniana, and proposed a possible role for cyclic electron flow (CEF) in ATP supply. In this study, we further exploited this hypothesis in both Chlorella sorokiniana C3 and the model green alga Chlamydomonas. We found that both the rate of CEF around photosystem I and the activity of thylakoid membrane-located ATP synthetase increased significantly during N starvation to drive ATP production. Furthermore, we demonstrated that the Chlamydomonas mutant pgrl1, which is deficient in PGRL1-mediated CEF, accumulated less neutral lipids and had reduced rates of CEF under N starvation. Further analysis revealed that Ca(2+) signaling regulates N starvation-induced neutral lipid biosynthesis in Chlamydomonas by increasing calmodulin activity and boosting the expression of the calcium sensor protein that regulates Pgrl1-mediated CEF. Thus, Ca(2+)-regulated CEF supplies ATP for N starvation-induced lipid biosynthesis in green alga. The increased CEF may re-equilibrate the ATP/NADPH balance and recycle excess light energy in photosystems to prevent photooxidative damage, suggesting Ca(2+)-regulated CEF also played a key role in protecting and sustaining photosystems. PMID:26450399

  6. Exercise and spirulina control non-alcoholic hepatic steatosis and lipid profile in diabetic Wistar rats

    PubMed Central

    2011-01-01

    Background Diabetes mellitus is associated with metabolic dysfunctions, including alterations in circulating lipid levels and fat tissue accumulation, which causes, among other pathologies, non-alcoholic fatty liver disease (NAFLD). Aim of the study The objective of this study was to analyse the effects of physical exercise and spirulina intake on the control of NAFLD in diabetic Wistar rats. Methods Diabetes was induced in the animals through intravenous administration of alloxan. The rats were divided into four groups: Diabetic Control (DC) - diabetic rats fed with a control diet and no physical exercise; Diabetic Spirulina (DS) - diabetic rats fed with a diet that included spirulina; Diabetic Spirulina and Exercise (DSE) - diabetic rats fed with a diet that included Spirulina and that exercised; and Diabetic Exercise (DE) - diabetic rats fed with a control diet and that exercised. Results The groups DS, DSE, and DE presented lower plasma concentrations of LDL cholesterol than DC, as well as lower levels of total liver lipids in groups DS, DSE, and DE in comparison to DC. Conclusion Thus, spirulina appears to be effective in reducing total circulating levels of LDL-cholesterol and hepatic lipids, alone or in conjunction with physical exercise in diabetic rats. PMID:21569626

  7. Process and Data Flow Control in KLOE

    NASA Astrophysics Data System (ADS)

    Pasqualucci, E.; KLOE Collaboration

    2001-10-01

    The core of the KLOE distributed event building system is a switched network. The online processes are distributed over a large set of processors in this network. All processes have to change coherently their state of activity as a consequence of local or remote commands. A fast and reliable message system based on the SNMP protocol has been developed. A command server has been implemented as a non privileged daemon able to respond to "set" and "get" queries on private SNMP variables. This process is able to convert remote set operations into local commands and to map automatically an SNMP subtree on a user-defined set of process variables. Process activity can be continuously monitored by remotely accessing their variables by means of the command server. Only the command server is involved in these operations, without disturbing the process flow. Subevents coming from subdetectors are sent to different nodes of a computing farm for the last stage of event building. Based on features of the SNMP protocol and of the KLOE message system, the Data Flow Control System (DFC) is able to rapidly redirect network traffic, keeping in account the dynamics of the whole DAQ system in order to assure coherent subevent addressing in an asynchronous "push" architecture, without introducing dead time. The KLOE DFC is currently working in the KLOE DAQ system. Its main characteristics and performance are discussed.

  8. Optimal Control of Flows in Moving Domains

    NASA Astrophysics Data System (ADS)

    Protas, Bartosz; Liao, Wenyuan; Glander, Donn

    2006-11-01

    This investigation concerns adjoint--based optimization of viscous incompressible flows (the Navier-Stokes problem) coupled with heat conduction involving change of phase (the Stefan problem) and occurring in domains with moving boundaries such as the free and solidification surfaces. This problem is motivated by optimization of advanced welding techniques used in automotive manufacturing. We characterize the sensitivity of a suitable cost functional defined for the system with respect to control (the heat input) using adjoint equations. Given that the shape of the domain is also a dependent variable, characterizing sensitivities necessitates the introduction of ``non-cylindrical'' calculus required to differentiate a cost functional defined on a variable domain. As a result, unlike the forward problem, the adjoint system is defined on a domain with a predetermined evolution in time and also involves ordinary differential equations defined on the domain boundary (``the adjoint transverse system''). We will discuss certain computational issues related to numerical solution of such adjoint problems.

  9. Adjustable flow rate controller for polymer solutions

    DOEpatents

    Jackson, Kenneth M.

    1981-01-01

    An adjustable device for controlling the flow rate of polymer solutions which results in only little shearing of the polymer molecules, said device comprising an inlet manifold, an outlet manifold, a plurality of tubes capable of providing communication between said inlet and outlet manifolds, said tubes each having an internal diameter that is smaller than that of the inlet manifold and large enough to insure that viscosity of the polymer solution passing through each said tube will not be reduced more than about 25 percent, and a valve associated with each tube, said valve being capable of opening or closing communication in that tube between the inlet and outlet manifolds, each said valve when fully open having a diameter that is substantially at least as great as that of the tube with which it is associated.

  10. Laminar flow control SPF/08 feasibility demonstration

    NASA Technical Reports Server (NTRS)

    Ecklund, R. C.; Williams, N. R.

    1981-01-01

    The feasibility of applying superplastic forming/diffusion bonding (SPF/DB) technology to laminar flow control (LFC) system concepts was demonstrated. Procedures were developed to produce smooth, flat titanium panels, using thin -0.016 inch sheets, meeting LFC surface smoothness requirements. Two large panels 28 x 28 inches were fabricated as final demonstration articles. The first was flat on the top and bottom sides demonstrating the capability of the tooling and the forming and diffusion bonding procedures to produce flat, defect free surfaces. The second panel was configurated for LFC porous panel treatment by forming channels with dimpled projections on the top side. The projections were machined away leaving holes extending into the panel. A perforated titanium sheet was adhesively bonded over this surface to complete the LFC demonstration panel. The final surface was considered flat enough to meet LFC requirements for a jet transport aircraft in cruising flight.

  11. Toward a laminar-flow-control transport

    NASA Technical Reports Server (NTRS)

    Sturgeon, R. F.

    1978-01-01

    Analyses were conducted to define a practical design for an advanced technology laminar flow control (LRC) transport for initial passenger operation in the early 1990's. Mission requirements, appropriate design criteria, and level of technology for the study aircraft were defined. The characteristics of the selected configuration were established, aircraft and LFC subsystems compatible with the mission requirements were defined, and the aircraft was evaluated in terms of fuel efficiency. A wing design integrating the LFC ducting and metering system into advanced composite wing structure was developed, manufacturing procedures for the surface panel design were established, and environmental and structural testing of surface panel components were conducted. Test results revealed a requirement for relatively minor changes in the manufacturing procedures employed, but have shown the general compatibility of both the selected design and the use of composite materials with the requirements of LFC wing surface panels.

  12. Laminar flow control perforated wing panel development

    NASA Technical Reports Server (NTRS)

    Fischler, J. E.

    1986-01-01

    Many structural concepts for a wing leading edge laminar flow control hybrid panel were analytically investigated. After many small, medium, and large tests, the selected design was verified. New analytic methods were developed to combine porous titanium sheet bonded to a substructure of fiberglass and carbon/epoxy cloth. At -65 and +160 F test conditions, the critical bond of the porous titanium to the composite failed at lower than anticipated test loads. New cure cycles, design improvements, and test improvements significantly improved the strength and reduced the deflections from thermal and lateral loadings. The wave tolerance limits for turbulence were not exceeded. Consideration of the beam column midbay deflections from the combinations of the axial and lateral loadings and thermal bowing at -65 F, room temperature, and +160 F were included. Many lap shear tests were performed at several cure cycles. Results indicate that sufficient verification was obtained to fabricate a demonstration vehicle.

  13. Synchronization trigger control system for flow visualization

    NASA Technical Reports Server (NTRS)

    Chun, K. S.

    1987-01-01

    The use of cinematography or holographic interferometry for dynamic flow visualization in an internal combustion engine requires a control device that globally synchronizes camera and light source timing at a predefined shaft encoder angle. The device is capable of 0.35 deg resolution for rotational speeds of up to 73 240 rpm. This was achieved by implementing the shaft encoder signal addressed look-up table (LUT) and appropriate latches. The developed digital signal processing technique achieves 25 nsec of high speed triggering angle detection by using direct parallel bit comparison of the shaft encoder digital code with a simulated angle reference code, instead of using angle value comparison which involves more complicated computation steps. In order to establish synchronization to an AC reference signal whose magnitude is variant with the rotating speed, a dynamic peak followup synchronization technique has been devised. This method scrutinizes the reference signal and provides the right timing within 40 nsec. Two application examples are described.

  14. Macrophage-derived lipid agonists of PPAR-α as intrinsic controllers of inflammation.

    PubMed

    Pontis, Silvia; Ribeiro, Alison; Sasso, Oscar; Piomelli, Daniele

    2016-01-01

    Macrophages are multi-faceted phagocytic effector cells that derive from circulating monocytes and undergo differentiation in target tissues to regulate key aspects of the inflammatory process. Macrophages produce and degrade a variety of lipid mediators that stimulate or suppress pain and inflammation. Among the analgesic and anti-inflammatory lipids released from these cells are the fatty acid ethanolamides (FAEs), which produce their effects by engaging nuclear peroxisome proliferator activated receptor-α (PPAR-α). Two members of this lipid family, palmitoylethanolamide (PEA) and oleoylethanolamide (OEA), have recently emerged as important intrinsic regulators of nociception and inflammation. These substances are released from the membrane precursor, N-acylphosphatidylethanolamine (NAPE), by the action of a NAPE-specific phospholipase D (NAPE-PLD), and in macrophage are primarily deactivated by the lysosomal cysteine amidase, N-acylethanolamine acid amidase (NAAA). NAPE-PLD and NAAA regulate FAE levels, exerting a tight control over the ability of these lipid mediators to recruit PPAR-α and attenuate the inflammatory response. This review summarizes recent findings on the contribution of the FAE-PPAR-α signaling complex in inflammation, and on NAAA inhibition as a novel mechanistic approach to treat chronic inflammatory disorders. PMID:26585314

  15. Dissipative control of energy flow in interconnected systems

    NASA Technical Reports Server (NTRS)

    Kishimoto, Y.; Bernstein, D. S.; Hall, S. R.

    1993-01-01

    Dissipative energy flow controllers are designed for interconnected modal subsystems. Active feedback controllers for vibration suppression are then viewed as either an additional subsystem or a dissipative coupling. These controllers, which are designed by the LQG positive real control approach, maximize energy flow from a specified modal subsystem.

  16. Controllable occurrence of free-standing lipid membranes on nanograting structured supports.

    PubMed

    Peng, Po-Yu; Chiang, Po-Chieh; Chao, Ling

    2014-08-13

    Supported lipid bilayers (SLBs) have been widely used to study protein-lipid membrane interactions because their planar geometry is suitable for many surface analysis tools. However, the friction coupling between the support and the membrane can influence the properties of biomolecules in the membrane. Many studies have attempted to span SLBs over nanostructured supports to create free-standing regions in SLBs for biosensor applications. However, membranes following the support surface contour are more frequently observed than are free-standing membranes on structured supports, indicating that the parameter range suitable for formation of free-standing SLBs might be narrow and more information is necessary to understand the required conditions. The objective of this study was to estimate the system energies of free-standing and contour-following membrane states and determine which state is the most energetically favorable under various conditions. For a lipid membrane preferring to stay close to the support, an energy reward occurs when they are in close proximity; however, increasing the contact area on a structured surface can result in an energy penalty because of the bending of the lipid bilayer. Whether the energy reward or the energy penalty dominates could determine the membrane state. We used the extended Derjaguin-Landau-Verwey-Overbeek (DLVO) theory and the Helfrich bending theory to relate the energy sizes to experimentally controllable parameters. We experimentally examined whether the membrane state followed the model prediction when we used various buffer ionic strengths, various lipid types, and nanograting supports with three different geometries. Because it is difficult to observe the experimental membrane state directly at the nanoscale, we developed a method to use the fluorescence recovery shape change after photobleaching to distinguish experimental membrane states at the micrometer scale. Our experimental results closely matched the

  17. Advanced stability theory analyses for laminar flow control

    NASA Technical Reports Server (NTRS)

    Orszag, S. A.

    1980-01-01

    Recent developments of the SALLY computer code for stability analysis of laminar flow control wings are summarized. Extensions of SALLY to study three dimensional compressible flows, nonparallel and nonlinear effects are discussed.

  18. Self-regulating flow control device

    DOEpatents

    Humphreys, Duane A.

    1984-01-01

    A variable, self-regulating valve having a hydraulic loss coefficient proportional to a positive exponential power of the flow rate. The device includes two objects in a flow channel and structure which assures that the distance between the two objects is an increasing function of the flow rate. The range of spacing between the objects is such that the hydraulic resistance of the valve is an increasing function of the distance between the two objects so that the desired hydraulic loss coefficient as a function of flow rate is obtained without variation in the flow area.

  19. Metastability in lipid based particles exhibits temporally deterministic and controllable behavior

    NASA Astrophysics Data System (ADS)

    Jacoby, Guy; Cohen, Keren; Barkan, Kobi; Talmon, Yeshayahu; Peer, Dan; Beck, Roy

    2015-03-01

    The metastable-to-stable phase-transition is commonly observed in many fields of science, as an uncontrolled independent process, highly sensitive to microscopic fluctuations. In particular, self-assembled lipid suspensions exhibit phase-transitions, where the underlying driving mechanisms and dynamics are not well understood. Here we describe a study of the phase-transition dynamics of lipid-based particles, consisting of mixtures of dilauroylphosphatidylethanolamine (DLPE) and dilauroylphosphatidylglycerol (DLPG), exhibiting a metastable liquid crystalline-to-stable crystalline phase transition upon cooling from 60°C to 37°C. Surprisingly, unlike classically described metastable-to-stable phase transitions, the manner in which recrystallization is delayed by tens of hours is robust, predetermined and controllable. Our results show that the delay time can be manipulated by changing lipid stoichiometry, changing solvent salinity, adding an ionophore, or performing consecutive phase-transitions. Moreover, the delay time distribution indicates a deterministic nature. We suggest that the non-stochastic physical mechanism responsible for the delayed recrystallization involves several rate-affecting processes, resulting in a controllable, non-independent metastability. A qualitative model is proposed to describe the structural reorganization during the phase transition.

  20. Metastability in lipid based particles exhibits temporally deterministic and controllable behavior

    PubMed Central

    Jacoby, Guy; Cohen, Keren; Barkan, Kobi; Talmon, Yeshayahu; Peer, Dan; Beck, Roy

    2015-01-01

    The metastable-to-stable phase-transition is commonly observed in many fields of science, as an uncontrolled independent process, highly sensitive to microscopic fluctuations. In particular, self-assembled lipid suspensions exhibit phase-transitions, where the underlying driving mechanisms and dynamics are not well understood. Here we describe a study of the phase-transition dynamics of lipid-based particles, consisting of mixtures of dilauroylphosphatidylethanolamine (DLPE) and dilauroylphosphatidylglycerol (DLPG), exhibiting a metastable liquid crystalline-to-stable crystalline phase transition upon cooling from 60°C to 37°C. Surprisingly, unlike classically described metastable-to-stable phase transitions, the manner in which recrystallization is delayed by tens of hours is robust, predetermined and controllable. Our results show that the delay time can be manipulated by changing lipid stoichiometry, changing solvent salinity, adding an ionophore, or performing consecutive phase-transitions. Moreover, the delay time distribution indicates a deterministic nature. We suggest that the non-stochastic physical mechanism responsible for the delayed recrystallization involves several rate-affecting processes, resulting in a controllable, non-independent metastability. A qualitative model is proposed to describe the structural reorganization during the phase transition. PMID:25820650

  1. KSRP is critical in governing hepatic lipid metabolism through controlling Per2 expression

    PubMed Central

    Chou, Chu-Fang; Zhu, Xiaolin; Lin, Yi-Yu; Gamble, Karen L.; Garvey, W. Timothy; Chen, Ching-Yi

    2015-01-01

    Hepatic lipid metabolism is controlled by integrated metabolic pathways. Excess accumulation of hepatic TG is a hallmark of nonalcoholic fatty liver disease, which is associated with obesity and insulin resistance. Here, we show that KH-type splicing regulatory protein (KSRP) ablation reduces hepatic TG levels and diet-induced hepatosteatosis. Expression of period 2 (Per2) is increased during the dark period, and circadian oscillations of several core clock genes are altered with a delayed phase in Ksrp−/− livers. Diurnal expression of some lipid metabolism genes is also disturbed with reduced expression of genes involved in de novo lipogenesis. Using primary hepatocytes, we demonstrate that KSRP promotes decay of Per2 mRNA through an RNA-protein interaction and show that increased Per2 expression is responsible for the phase delay in cycling of several clock genes in the absence of KSRP. Similar to Ksrp−/− livers, both expression of lipogenic genes and intracellular TG levels are also reduced in Ksrp−/− hepatocytes due to increased Per2 expression. Using heterologous mRNA reporters, we show that the AU-rich element-containing 3′ untranslated region of Per2 is responsible for KSRP-dependent mRNA decay. These findings implicate that KSRP is an important regulator of circadian expression of lipid metabolism genes in the liver likely through controlling Per2 mRNA stability. PMID:25514904

  2. Effects of Vaccinium Berries on Serum Lipids: A Meta-Analysis of Randomized Controlled Trials

    PubMed Central

    Zhu, Yitong; Miao, Ya; Meng, Zheying; Zhong, Yuan

    2015-01-01

    The beneficial effects of anthocyanins consumption on cardiovascular risk are supported by mechanistic and epidemiologic evidence. In order to explore the effects of Vaccinium berries rich in anthocyanins on serum lipids, we conducted a meta-analysis of relevant randomized controlled trials (RCTs). Sixteen studies with 1109 subjects were included in this meta-analysis. Significant heterogeneity confirmed differential effects between Vaccinium subclasses. The whortleberry group is significantly superior to placebo in lipids improvement. Besides, bilberry groups show significant differences in reducing LDL-C and increasing HDL-C in comparison with other treatments. For many of the other subgroups and comparison arms, there was insufficient evidence to draw conclusions about efficacy. PMID:26345230

  3. Interactions between Starch, Lipids, and Proteins in Foods: Microstructure Control for Glycemic Response Modulation.

    PubMed

    Parada, Javier; Santos, Jose L

    2016-10-25

    In real food, starch is usually forming part of a matrix with lipids and proteins. However, research on this ternary system and interactions between such food components has been scarce so far. The control of food microstructure is crucial to determine the product properties, including sensorial and nutritionals ones. This paper reviews the microstructural principles of interactions between starch, lipids, and proteins in foods as well as their effect on postprandial glycemic response, considering human intrinsic differences on postprandial glycemic responses. Several lines of research support the hypothesis that foods without rapidly digestible starch will not mandatorily generate the lowest postprandial glycemic response, highlighting that the full understanding of food microstructure, which modulates starch digestion, plays a key role on food design from a nutritional viewpoint. PMID:25831145

  4. Precise and millidegree stable temperature control for fluorescence imaging: Application to phase transitions in lipid membranes

    PubMed Central

    Farkas, Elaine R.; Webb, Watt W.

    2010-01-01

    We present the design of a custom temperature-controlled chamber suitable for water or oil immersion fluorescence microscopy and its application to phase behavior in lipid bilayer vesicles. The apparatus is self-contained and portable, suitable for multiuser microscopy facilities. It offers a higher temperature resolution and stability than any comparable commercial apparatus, on the order of millidegrees. We demonstrate the utility of the system in the study of miscibility transitions in model membranes. The temperature-dependent phase behavior of model membrane systems that display liquid-ordered (Lo) phase coexistence with the liquid-disordered (Ld) phase is relevant to understanding the existence of heterogeneities in biological cell plasma membranes, ubiquitously termed “lipid rafts.” PMID:20886984

  5. The role of porous nanostructure in controlling lipase-mediated digestion of lipid loaded into silica particles.

    PubMed

    Joyce, Paul; Tan, Angel; Whitby, Catherine P; Prestidge, Clive A

    2014-03-18

    The rate and extent of lipolysis, the breakdown of fat into molecules that can be absorbed into the bloodstream, depend on the interfacial composition and structure of lipid (fat) particles. A novel method for controlling the interfacial properties is to load the lipid into porous colloidal particles. We report on the role of pore nanostructure and surface coverage in controlling the digestion kinetics of medium-chain and long-chain triglycerides loaded into porous silica powders of different particle size, porosity, and hydrophobicity/hydrophilicity. An in vitro lipolysis model was used to measure digestion kinetics of lipid by pancreatic lipase, a digestive enzyme. The rate and extent of lipid digestion were significantly enhanced when a partial monolayer of lipid was loaded in porous hydrophilic silica particles compared to a submicrometer lipid-in-water emulsion or a coarse emulsion. The inhibitory effect of digestion products was clearly evident for digestion from a submicrometer emulsion and coarse emulsion. This effect was minimal, however, in the two silica-lipid systems. Lipase action was inhibited for lipid loaded in the hydrophobic silica and considered due to the orientation of lipase adsorption on the methylated silica surface. Thus, hydrophilic silica promotes enhanced digestion kinetics, whereas hydrophobic silica exerts an inhibitory effect on hydrolysis. Evaluation of digestion kinetics enabled the mechanism for enhanced rate of lipolysis in silica-lipid systems to be derived and detailed. These investigations provide valuable insights for the optimization of smart food microparticles and lipid-based drug delivery systems based on lipid excipients and porous nanoparticles. PMID:24552363

  6. Effect of Probiotics on Blood Lipid Concentrations: A Meta-Analysis of Randomized Controlled Trials.

    PubMed

    Cho, Young Ae; Kim, Jeongseon

    2015-10-01

    Previous clinical studies have reported mixed results regarding the effect of probiotics on lipid metabolism. Therefore, we conducted a meta-analysis of randomized controlled trials to quantify the direction and magnitude of the potential effect of probiotics on blood lipid concentrations.Eligible studies were randomized, placebo-controlled trials whose interventions were probiotic products containing live bacteria. The studies reported net changes in lipid profiles (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides) and their associated standard deviations (or the data to calculate them). The probiotic products did not contain prebiotics or other active ingredients, and the full article was accessible in English.The pooled mean net change in lipid profiles and 95% confidence intervals (95% CIs) were calculated. Q statistics and I were calculated to examine heterogeneity. Potential sources of heterogeneity were investigated via subgroup and sensitivity analyses, and publication biases were estimated.A total of 30 randomized controlled trials with 1624 participants (828 in intervention groups and 796 in placebo groups) were included in this analysis. Subjects treated with probiotics demonstrated reduced total cholesterol and LDL cholesterol compared to control subjects by 7.8 mg/dL (95% CI: -10.4, -5.2) and 7.3 mg/dL (95% CI: -10.1, -4.4), respectively. There was no significant effect of probiotics on HDL cholesterol or triglycerides. The effect of probiotics on total cholesterol and LDL cholesterol depended on a variety of factors. The significant effects were greater for higher baseline total cholesterol levels, longer treatment durations, and certain probiotic strains. In addition, these associations seem stronger in studies supported by probiotics companies.The studies included in this meta-analysis showed significant heterogeneity as indicated by the Q statistics and I. In addition, industry sponsorship may affect study findings

  7. Effect of Chromium Supplementation on Glucose Metabolism and Lipids: A Systematic Review with Meta-Analysis of Randomized Controlled Trials

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Objective. A systematic review of the effect of chromium supplementation on glucose metabolism and lipid levels. Research Design and Methods. Literature search conducted in MEDLINE and Commonwealth Agricultural Bureau. Eligible studies were English language randomized controlled trials of chromium ...

  8. Sesame fractions and lipid profiles: a systematic review and meta-analysis of controlled trials.

    PubMed

    Khalesi, Saman; Paukste, Ernesta; Nikbakht, Elham; Khosravi-Boroujeni, Hossein

    2016-03-14

    Increased plasma lipid profiles are among the most important risk factors of CHD and stroke. Sesame contains considerable amounts of vitamin E, MUFA, fibre and lignans, which are thought to be associated with its plasma lipid-lowering properties. This study aimed to systematically review the evidence and identify the effects of sesame consumption on blood lipid profiles using a meta-analysis of controlled trials. PubMed, CINAHL and Cochrane Library databases were searched (from 1960 to May 2015). A total of ten controlled trials were identified based on the eligibility criteria. Both the Cochrane Collaboration tool and the Rosendal scale were used to assess the risk of bias of the included studies. The meta-analysis results showed that consumption of sesame did not significantly change the concentrations of total blood cholesterol (-0·32 mmol/l; 95% CI -0·75, 0·11; P=0·14, I(2)=96%), LDL-cholesterol (-0·15 mmol/l; 95% CI -0·50, 0·19; P=0·39, I(2)=96%) or HDL-cholesterol (0·01 mmol/l; 95% CI -0·00, 0·02; P=0·16, I(2)=0%). However, a significant reduction was observed in serum TAG levels (-0·24 mmol/l; 95% CI -0·32, -0·15; P<0·001, I(2)=84%) after consumption of sesame. It was concluded that sesame consumption can significantly reduce blood TAG levels but there is insufficient evidence to support its hypocholesterolaemic effects. Further studies are required to determine the potential effect of sesame consumption on lipid profiles and cardiovascular risk factors. PMID:26758593

  9. Synthetic Capillaries to Control Microscopic Blood Flow

    PubMed Central

    Sarveswaran, K.; Kurz, V.; Dong, Z.; Tanaka, T.; Penny, S.; Timp, G.

    2016-01-01

    Capillaries pervade human physiology. The mean intercapillary distance is only about 100 μm in human tissue, which indicates the extent of nutrient diffusion. In engineered tissue the lack of capillaries, along with the associated perfusion, is problematic because it leads to hypoxic stress and necrosis. However, a capillary is not easy to engineer due to its complex cytoarchitecture. Here, it is shown that it is possible to create in vitro, in about 30 min, a tubular microenvironment with an elastic modulus and porosity consistent with human tissue that functionally mimicks a bona fide capillary using “live cell lithography”(LCL) to control the type and position of cells on a composite hydrogel scaffold. Furthermore, it is established that these constructs support the forces associated with blood flow, and produce nutrient gradients similar to those measured in vivo. With LCL, capillaries can be constructed with single cell precision—no other method for tissue engineering offers such precision. Since the time required for assembly scales with the number of cells, this method is likely to be adapted first to create minimal functional units of human tissue that constitute organs, consisting of a heterogeneous population of 100–1000 cells, organized hierarchically to express a predictable function. PMID:26905751

  10. Synthetic Capillaries to Control Microscopic Blood Flow

    NASA Astrophysics Data System (ADS)

    Sarveswaran, K.; Kurz, V.; Dong, Z.; Tanaka, T.; Penny, S.; Timp, G.

    2016-02-01

    Capillaries pervade human physiology. The mean intercapillary distance is only about 100 μm in human tissue, which indicates the extent of nutrient diffusion. In engineered tissue the lack of capillaries, along with the associated perfusion, is problematic because it leads to hypoxic stress and necrosis. However, a capillary is not easy to engineer due to its complex cytoarchitecture. Here, it is shown that it is possible to create in vitro, in about 30 min, a tubular microenvironment with an elastic modulus and porosity consistent with human tissue that functionally mimicks a bona fide capillary using “live cell lithography”(LCL) to control the type and position of cells on a composite hydrogel scaffold. Furthermore, it is established that these constructs support the forces associated with blood flow, and produce nutrient gradients similar to those measured in vivo. With LCL, capillaries can be constructed with single cell precision—no other method for tissue engineering offers such precision. Since the time required for assembly scales with the number of cells, this method is likely to be adapted first to create minimal functional units of human tissue that constitute organs, consisting of a heterogeneous population of 100-1000 cells, organized hierarchically to express a predictable function.

  11. Synthetic Capillaries to Control Microscopic Blood Flow.

    PubMed

    Sarveswaran, K; Kurz, V; Dong, Z; Tanaka, T; Penny, S; Timp, G

    2016-01-01

    Capillaries pervade human physiology. The mean intercapillary distance is only about 100 μm in human tissue, which indicates the extent of nutrient diffusion. In engineered tissue the lack of capillaries, along with the associated perfusion, is problematic because it leads to hypoxic stress and necrosis. However, a capillary is not easy to engineer due to its complex cytoarchitecture. Here, it is shown that it is possible to create in vitro, in about 30 min, a tubular microenvironment with an elastic modulus and porosity consistent with human tissue that functionally mimicks a bona fide capillary using "live cell lithography"(LCL) to control the type and position of cells on a composite hydrogel scaffold. Furthermore, it is established that these constructs support the forces associated with blood flow, and produce nutrient gradients similar to those measured in vivo. With LCL, capillaries can be constructed with single cell precision-no other method for tissue engineering offers such precision. Since the time required for assembly scales with the number of cells, this method is likely to be adapted first to create minimal functional units of human tissue that constitute organs, consisting of a heterogeneous population of 100-1000 cells, organized hierarchically to express a predictable function. PMID:26905751

  12. Flow Control Device Evaluation for an Internal Flow with an Adverse Pressure Gradient

    NASA Technical Reports Server (NTRS)

    Jenkins, Luther N.; Gorton, Susan Althoff; Anders, Scott G.

    2002-01-01

    The effectiveness of several active and passive devices to control flow in an adverse pressure gradient with secondary flows present was evaluated in the 15 Inch Low Speed Tunnel at NASA Langley Research Center. In this study, passive micro vortex generators, micro bumps, and piezoelectric synthetic jets were evaluated for their flow control characteristics using surface static pressures, flow visualization, and 3D Stereo Digital Particle Image Velocimetry. Data also were acquired for synthetic jet actuators in a zero flow environment. It was found that the micro vortex generator is very effective in controlling the flow environment for an adverse pressure gradient, even in the presence of secondary vortical flow. The mechanism by which the control is effected is a re-energization of the boundary layer through flow mixing. The piezoelectric synthetic jet actuators must have sufficient velocity output to produce strong longitudinal vortices if they are to be effective for flow control. The output of these devices in a laboratory or zero flow environment will be different than the output in a flow environment. In this investigation, the output was higher in the flow environment, but the stroke cycle in the flow did not indicate a positive inflow into the synthetic jet.

  13. Boundary-layer-ingesting inlet flow control system

    NASA Technical Reports Server (NTRS)

    Owens, Lewis R. (Inventor); Allan, Brian G. (Inventor)

    2010-01-01

    A system for reducing distortion at the aerodynamic interface plane of a boundary-layer-ingesting inlet using a combination of active and passive flow control devices is disclosed. Active flow control jets and vortex generating vanes are used in combination to reduce distortion across a range of inlet operating conditions. Together, the vortex generating vanes can reduce most of the inlet distortion and the active flow control jets can be used at a significantly reduced control jet mass flow rate to make sure the inlet distortion stays low as the inlet mass flow rate varies. Overall inlet distortion, measured and described as average SAE circumferential distortion descriptor, was maintained at a value of 0.02 or less. Advantageous arrangements and orientations of the active flow control jets and the vortex generating vanes were developed using computational fluid dynamics simulations and wind tunnel experimentations.

  14. The GTPase ARFRP1 controls the lipidation of chylomicrons in the Golgi of the intestinal epithelium.

    PubMed

    Jaschke, Alexander; Chung, Bomee; Hesse, Deike; Kluge, Reinhart; Zahn, Claudia; Moser, Markus; Petzke, Klaus-Jürgen; Brigelius-Flohé, Regina; Puchkov, Dmytro; Koepsell, Hermann; Heeren, Joerg; Joost, Hans-Georg; Schürmann, Annette

    2012-07-15

    The uptake and processing of dietary lipids by the small intestine is a multistep process that involves several steps including vesicular and protein transport. The GTPase ADP-ribosylation factor-related protein 1 (ARFRP1) controls the ARF-like 1 (ARL1)-mediated Golgi recruitment of GRIP domain proteins which in turn bind several Rab-GTPases. Here, we describe the essential role of ARFRP1 and its interaction with Rab2 in the assembly and lipidation of chylomicrons in the intestinal epithelium. Mice lacking Arfrp1 specifically in the intestine (Arfrp1(vil-/-)) exhibit an early post-natal growth retardation with reduced plasma triacylglycerol and free fatty acid concentrations. Arfrp1(vil-/-) enterocytes as well as Arfrp1 mRNA depleted Caco-2 cells absorbed fatty acids normally but secreted chylomicrons with a markedly reduced triacylglycerol content. In addition, the release of apolipoprotein A-I (ApoA-I) was dramatically decreased, and ApoA-I accumulated in the Arfrp1(vil-/-) epithelium, where it predominantly co-localized with Rab2. The release of chylomicrons from Caco-2 was markedly reduced after the suppression of Rab2, ARL1 and Golgin-245. Thus, the GTPase ARFRP1 and its downstream proteins are required for the lipidation of chylo-microns and the assembly of ApoA-I to these particles in the Golgi of intestinal epithelial cells. PMID:22505585

  15. Advanced stability analysis for laminar flow control

    NASA Technical Reports Server (NTRS)

    Orszag, S. A.

    1981-01-01

    Five classes of problems are addressed: (1) the extension of the SALLY stability analysis code to the full eighth order compressible stability equations for three dimensional boundary layer; (2) a comparison of methods for prediction of transition using SALLY for incompressible flows; (3) a study of instability and transition in rotating disk flows in which the effects of Coriolis forces and streamline curvature are included; (4) a new linear three dimensional instability mechanism that predicts Reynolds numbers for transition to turbulence in planar shear flows in good agreement with experiment; and (5) a study of the stability of finite amplitude disturbances in axisymmetric pipe flow showing the stability of this flow to all nonlinear axisymmetric disturbances.

  16. Fluid Flow Control with Transformation Media

    NASA Astrophysics Data System (ADS)

    Urzhumov, Yaroslav A.; Smith, David R.

    2011-08-01

    We introduce a new concept for the manipulation of fluid flow around three-dimensional bodies. Inspired by transformation optics, the concept is based on a mathematical idea of coordinate transformations and physically implemented with anisotropic porous media permeable to the flow of fluids. In two situations—for an impermeable object placed either in a free-flowing fluid or in a fluid-filled porous medium—we show that the object can be coated with an inhomogeneous, anisotropic permeable medium, such as to preserve the flow that would have existed in the absence of the object. The proposed fluid flow cloak eliminates downstream wake and compensates viscous drag, hinting at the possibility of novel propulsion techniques.

  17. Topographic Controls on Landslide and Debris-Flow Mobility

    NASA Astrophysics Data System (ADS)

    McCoy, S. W.; Pettitt, S.

    2014-12-01

    Regardless of whether a granular flow initiates from failure and liquefaction of a shallow landslide or from overland flow that entrains sediment to form a debris flow, the resulting flow poses hazards to downslope communities. Understanding controls on granular-flow mobility is critical for accurate hazard prediction. The topographic form of granular-flow paths can vary significantly across different steeplands and is one of the few flow-path properties that can be readily altered by engineered control structures such as closed-type check dams. We use grain-scale numerical modeling (discrete element method simulations) of free-surface, gravity-driven granular flows to investigate how different topographic profiles with the same mean slope and total relief can produce notable differences in flow mobility due to strong nonlinearities inherent to granular-flow dynamics. We describe how varying the profile shape from planar, to convex up, to concave up, as well how varying the number, size, and location of check dams along a flow path, changes flow velocity, thickness, discharge, energy dissipation, impact force and runout distance. Our preliminary results highlight an important path dependence for this nonlinear system, show that caution should be used when predicting flow dynamics from path-averaged properties, and provide some mechanics-based guidance for engineering control structures.

  18. Fatty Acid Desaturase 1 (FADS1) Gene Polymorphisms Control Human Hepatic Lipid Composition

    PubMed Central

    Wang, Libo; Athinarayanan, Shaminie; Jiang, Guanglong; Chalasani, Naga; Zhang, Min; Liu, Wanqing

    2014-01-01

    Fatty Acid Desaturase (FADS) genes and their variants have been associated with multiple metabolic phenotypes including liver enzymes and hepatic fat accumulation but the detailed mechanism remains unclear. We aimed to delineate the role of FADSs in modulating lipid composition in human liver. We performed a targeted lipidomic analysis of a variety of phospholipids, sphingolipids and ceramides among 154 human liver tissue samples. The associations between previously Genome-wide Association Studies (GWAS)-identified six FADS single nucleotide polymorphisms (SNPs) and these lipid levels as well as total hepatic fat content (HFC) were tested. The potential function of these SNPs in regulating transcription of 3 FADS genes (FADS1, FADS2 and FADS3) in the locus was also investigated. We found that while these SNPs were in high linkage disequilibrium (r2 >0.8), the rare alleles of these SNPs were consistently and significantly associated with the accumulation of multiple very-long-chain fatty acids (VLCFAs), with C47H85O13P (C36:4), a phosphatidylinositol (PI) and C43H80O8PN (C38:3), a phosphatidylethanolamine (PE) reached the Bonferroni corrected significance (p<3×10−4). Meanwhile, these SNPs were significantly associated with increased ratios between the more saturated and relatively less saturated forms of VLCFAs, especially between PEs, PIs and phosphatidylcholines (PCs) (p≤3.5×10−6). These alleles were also associated with increased total HFC (p<0.05). Further analyses revealed that these alleles were associated with decreased hepatic expression of FADS1 (p=0.0018 for rs174556), but not FADS2 or FADS3 (p>0.05). Conclusion Our findings revealed critical insight into the mechanism underlying FADS1 and its polymorphisms in modulating hepatic lipid deposition by altering gene transcription and controlling lipid composition in human livers. PMID:25123259

  19. Effect of Medium pH on Rhodosporidium toruloides NCYC 921 Carotenoid and Lipid Production Evaluated by Flow Cytometry.

    PubMed

    Dias, Carla; Silva, Corália; Freitas, Claudia; Reis, Alberto; da Silva, Teresa Lopes

    2016-07-01

    The effect of the culture medium pH (3.5-6.0) on the carotenoid and lipid (as fatty acids) production by the yeast Rhodosporidium toruloides NCYC 921 was studied. Flow cytometry was used to evaluate the yeast's physiological response to different culture medium pH values. The yeast biomass concentration and lipid content were maxima at pH 4.0 (5.90 g/L and 21.85 % w/w, respectively), while the maximum carotenoid content (63.37 μg/g) was obtained at pH 5.0. At the exponential phase, the yeast cell size and internal complexity were similar, at different medium pH. At the stationary phase, the yeast cell size and internal complexity decreased as the medium pH increased. At the exponential phase, the proportion of cells with polarized membranes was always high (>80 %) but at the stationary phase, the proportion of yeast cells with depolarized membranes was dominant (>65 %) and increased with the medium pH increase. The results here reported may contribute for yeast bioprocesses optimization. For the first time, multiparameter flow cytometry was used to evaluate the impact of medium pH changes on the yeast cell physiological status, specifically on the yeast membrane potential, membrane integrity, cell size and internal complexity. PMID:26945576

  20. Active Flow Control on a Low Reynolds Number Wing

    NASA Astrophysics Data System (ADS)

    Munson, Matthew; Gharib, Morteza

    2010-11-01

    Control of vortex formation has been shown to be a critical mechanism in some forms of animal flight. Flapping motions create advantageous flow structures which play a role in enhancing lift and increasing maneuverability. Active flow control may be capable of providing similar influence over vortex formation processes in fixed wing flight at small Reynolds numbers. Steady and pulsed mass injection strategies through simple slot actuators are used to explore the open-loop response of the flow around a simple low-aspect ratio wing. Flow dynamics and vortex formation will be quantitatively visualized with DPIV and flow forces will be simultaneously measured with a six-component balance.

  1. Isotopic Controls of Rainwater and Water Vapor on Mangrove Leaf Water and Lipid Biomarkers

    NASA Astrophysics Data System (ADS)

    Ladd, N.; Wolfshorndl, M.; Sachs, J. P.

    2015-12-01

    Hydrogen isotope ratios (2H/1H or δ2H) of sedimentary mangrove lipid biomarkers can be used as a proxy of past salinity and water isotopes. This approach is based on the observation that apparent 2H/1H fractionation between surface water and mangrove lipids increases with surface water salinity in six species of mangroves with different salt management strategies growing at sites spanning a range of relative humidities throughout Australia and Micronesia. In order to more robustly apply mangrove lipid δ2H as a paleoclimate proxy, we investigated the cause of the correlation between apparent 2H fractionation and salinity. We present results from two related experiments that assessed controls on isotopes of mangrove leaf water, the direct source of hydrogen in lipids: (1) Measurements of natural δ2H in precipitation, surface water, and mangrove tissue water from a series of lakes with varying salinity and water isotope composition in Palau, and (2) measurements of mangrove tissue water and treatment water from a controlled simulation in which mangroves were treated with artificial rain of varying isotopic composition. Rainwater 2H/1H fluctuations of 30‰ over a one-month period explain up to 65% of the variance in leaf water δ2H for Bruguiera gymnorhiza mangroves from Palau despite lake water isotope differences among sites of up to 35‰. This indicates that in humid tropical settings, leaf water isotopes are more closely related to those of precipitation and water vapor than to those of lake surface water, explaining the observed change in apparent fractionation in B. gymnorhiza lipids with salinity. The relationship between leaf water and rainwater isotopes may be due to either equilibration of leaf water with water vapor in the nearly saturated air or direct foliar uptake of rain and/or dew. Foliar uptake is an important water source for many plants, but has not been documented in mangroves. We tested the capacity for mangroves to perform this function by

  2. Optimization of Airfoil Design for Flow Control with Plasma Actuators

    NASA Astrophysics Data System (ADS)

    Williams, Theodore; Corke, Thomas; Cooney, John

    2011-11-01

    Using computer simulations and design optimization methods, this research examines the implementation of active flow control devices on wind turbine blades. Through modifications to blade geometry in order to maximize the effectiveness of flow control devices, increases in aerodynamic performance and control of aerodynamic performance are expected. Due to this compliant flow, an increase in the power output of wind turbines is able to be realized with minimal modification and investment to existing turbine blades. This is achieved through dynamic lift control via virtual camber control. Methods using strategic flow separation near the trailing edge are analyzed to obtain desired aerodynamic performance. FLUENT is used to determine the aerodynamic performance of potential turbine blade design, and the post-processing uses optimization techniques to determine an optimal blade geometry and plasma actuator operating parameters. This work motivates the research and development of novel blade designs with flow control devices that will be tested at Notre Dame's Laboratory for Enhanced Wind Energy Design.

  3. Development of Advanced Casing Treatments for Flow Control

    NASA Technical Reports Server (NTRS)

    Keith, Theo G., Jr.; Tsung, Fu-Lin

    2001-01-01

    Under the Base R&T and Ultra Efficient Engine Technology programs, the NASA-Goddard Space Flight Center Compressor Branch is investigating flow control strategies required to increase the loading and efficiency of core compressors while maintaining current levels of operability. Flow-control strategies being studied include advanced casing treatments, wall jet injection, and blade-tip injection for compressor stability enhancement, directed jets for surface boundary layer control, and vortex-generating devices. The use of computational fluid dynamics (CFD) simulations to assess the effectiveness of flow-control devices and to guide their design is a key element in this research. CFD simulations serve to screen potential flow-control concepts at a lower cost than executing physical experiments in turbomachinery facilities. CFD simulations also provide guidance in designing physical experiments for those flow control concepts, which appear promising.

  4. Thaw flow control for liquid heat transport systems

    DOEpatents

    Kirpich, Aaron S.

    1989-01-01

    In a liquid metal heat transport system including a source of thaw heat for use in a space reactor power system, the thaw flow throttle or control comprises a fluid passage having forward and reverse flow sections and a partition having a plurality of bleed holes therein to enable fluid flow between the forward and reverse sections. The flow throttle is positioned in the system relatively far from the source of thaw heat.

  5. Driven cavity simulation of turbomachine blade flows with vortex control

    NASA Technical Reports Server (NTRS)

    Athavale, M. M.; Przekwas, A. J.; Hendricks, R. C.

    1993-01-01

    This paper presents a computational study of the three-dimensional flows in a rotating cavity with clearance between cavity walls and lid wall. The objectives of this study is to understand the interaction mechanism between tip leakage and blade passage flows and to assess the means to control the flow pattern and pressure losses. The classes of problems addressed include: passage geometry, passage loading including lid velocity and anti-vortex strength, and placement necessary to provide flow control. The computational model is first validated on generic flow problems and then applied to a specific blade passage configuration. Results of parametric studies for secondary flow pattern control are analyzed, and practical means of vortex control are discussed.

  6. Pressurized tundish for controlling a continuous flow of molten metal

    DOEpatents

    Lewis, T.W.; Hamill, P.E. Jr.; Ozgu, M.R.; Padfield, R.C.; Rego, D.N.; Brita, G.P.

    1990-07-24

    A pressurized tundish for controlling a continuous flow of molten metal is characterized by having a pair of principal compartments, one being essentially unpressurized and receiving molten metal introduced thereto, and the other being adapted for maintaining a controlled gaseous pressure over the surface of the fluid metal therein, whereby, by controlling the pressure within the pressurized chamber, metal exiting from the tundish is made to flow continually and at a controlled rate. 1 fig.

  7. Pressurized tundish for controlling a continuous flow of molten metal

    DOEpatents

    Lewis, Thomas W.; Hamill, Jr., Paul E.; Ozgu, Mustafa R.; Padfield, Ralph C.; Rego, Donovan N.; Brita, Guido P.

    1990-01-01

    A pressurized tundish for controlling a continous flow of molten metal characterized by having a pair of principal compartments, one being essentially unpressurized and receiving molten metal introduced thereto, and the other being adapted for maintaining a controlled gaseous pressure over the surface of the fluid metal therein, whereby, by controlling the pressure within the pressurized chamber, metal exiting from the tundish is made to flow continually and at a controlled rate.

  8. Characterization, biorecognitive activity and stability of WGA grafted lipid nanostructures for the controlled delivery of Rifampicin.

    PubMed

    Pooja, Deep; Tunki, Lakshmi; Kulhari, Hitesh; Reddy, Bharathi B; Sistla, Ramakrishna

    2015-12-01

    Targeted nanomedicines improve the delivery of drugs by increasing the drug concentration at target site, protecting the premature degradation and releasing the encapsulated drug in controlled manner. To make rifampicin (RFN) delivery more effective, we designed and characterized wheat germ agglutinin (WGA) conjugated, RFN loaded solid-lipid nanoparticles (WRSN). Nanoparticles were prepared by solvent emulsification/evaporation and conjugated with fluorescein isothiocyanate-labeled WGA. Important characteristics, such as particle size, zeta potential, encapsulation efficiency, conjugation efficiency and in vitro drug release behavior, were investigated. WGA conjugation to the nanoparticles was confirmed by Fourier Transform Infrared (FTIR) analysis. Conjugation efficiency was determined by fluorescent spectroscopy and Bradford assay. RFN was released from nanoparticles via the diffusion-controlled, non-fickian and supercase II mechanism. A haemaglutination test confirmed that WGA retained its bio-recognition activity and sugar-binding specificity after it was coupled with the nanoparticles. In vitro experiments demonstrated that WRSN interacted more than non-conjugated nanoparticles with porcine mucin. WRSN were stable in the presence of electrolytes up to 1M concentration. Therefore, WGA-conjugated solid lipid nanoparticles could be a promising tool for the controlled delivery of RFN or other anti-tubercular drugs. PMID:26409629

  9. Flow Control and Hydro dynamic Instability

    NASA Astrophysics Data System (ADS)

    Kozlov, V. V.

    Scientific problems related to modern aeronautical engineering and dealing with basic properties of shear flows and the associated fluid mechanics phenomena are emphasized. In this context some recent experimental results on subsonic aerodynamics are considered.

  10. The art and science of flow control - case studies using flow visualization methods

    NASA Astrophysics Data System (ADS)

    Alvi, F. S.; Cattafesta, L. N., III

    2010-04-01

    Active flow control (AFC) has been the focus of significant research in the last decade. This is mainly due to the potentially substantial benefits it affords. AFC applications range from the subsonic to the supersonic (and beyond) regime for both internal and external flows. These applications are wide and varied, such as controlling flow transition and separation over various external components of the aircraft to active management of separation and flow distortion in engine components and over turbine and compressor blades. High-speed AFC applications include control of flow oscillations in cavity flows, supersonic jet screech, impinging jets, and jet-noise control. In this paper we review some of our recent applications of AFC through a number of case studies that illustrate the typical benefits as well as limitations of present AFC methods. The case studies include subsonic and supersonic canonical flowfields such as separation control over airfoils, control of supersonic cavity flows and impinging jets. In addition, properties of zero-net mass-flux (ZNMF) actuators are also discussed as they represent one of the most widely studied actuators used for AFC. In keeping with the theme of this special issue, the flowfield properties and their response to actuation are examined through the use of various qualitative and quantitative flow visualization methods, such as smoke, shadowgraph, schlieren, planar-laser scattering, and Particle image velocimetry (PIV). The results presented here clearly illustrate the merits of using flow visualization to gain significant insight into the flow and its response to AFC.

  11. Reliable and efficient hop-by-hop flow control

    NASA Astrophysics Data System (ADS)

    Ozveren, Cuneyt M.; Simcoe, Robert; Varghese, George

    1995-05-01

    Hop-by-hop flow control can be used to fairly share the bandwidth of a network among competing flows. No data is lost even in overload conditions; yet each flow gets access to the maximum throughput when the network is lightly loaded. However, some schemes for hop-by-hop flow control require too much memory; some of them are not resilient to errors. We propose a scheme for making hop-by-hop flow control resilient and show that it has advantages over the first several schemes proposed by Kung. We also describe a novel method for sharing the available buffers among the flows on a link; our scheme allows us to potentially reduce the memory requirement (or increase the number of flows that can be supported) by an order of magnitude. Most of the work is described in the context of an ATM network that uses credit-based flow control. However, our ideas extend to networks in which flows can be distinguished, and to rate-based flow control schemes.

  12. MAG-GATE System for Molten metal Flow Control

    SciTech Connect

    Richard D. Nathenson, P.E.

    2004-05-15

    The need for improved active flow control has been recognized as part of the Steel Industry Technology Roadmap. Under TRP 9808 for the American Iron and Steel Institute and the Department of Energy, Concept Engineering Group Inc. has developed MAG-GATE{trademark}, an electromagnetic system for active molten metal flow control. Two hot steel tests were successfully conducted in 2003 at the Whemco Foundry Division, Midland, PA. Approximately 110,000 pounds of 0.2% carbon steel were poured through the device subject to electromagnetic flow control. Excellent agreement between predicted and actual flow control was found. A survey of the molten metal flow control practices at 100 continuous casters in North America was also conducted in 2003. This report summarizes the results of the development program to date. Preliminary designs are described for the next step of a beta test at an operating billet/bloom or slab caster.

  13. Characterizing cryogenic propellant flow behavior through a cavitating venturi in comparison to alternative flow control mechanisms

    NASA Astrophysics Data System (ADS)

    Ingle, Marjorie Adele

    The work detailed is an investigation of the use of a cavitating venturi as both a flow control and metering device. This was achieved through the combination of actual experimentation and numerical modeling of the fluid behavior of both liquid water and liquid methane as it passes through the test article designed, developed, and validated here within this study. The discharge coefficient of the cavitating venturi was determined through weigh flow calibration testing to determine an average mass flow rate. Turbine flow meter flow rate readings were used as a point of comparison and the discharge coefficient was computed. The discharge coefficient was then implemented into the Bernoulli Equation along with experimental pressure and temperature data to again calculate mass flow rate through the cavitating venturi. The agreement of the venturi flow rate data to that of the turbine flow meter effectively established its applicability as a passive flow control and metering feature. A preliminary CFD cavitation model was developed and validated for cavitating water flow regimes using ANSYS FLUENT. Agreement between mass flow rates obtained from the model to experimental data for cavitating water flow indicates that deviations in results for liquid methane analysis from experimental results could simply be the result of insufficiently defined fluid characteristics in the ANSYS FLUENT materials database. SEM surface roughness analysis of a secondary test article indicated that the default average surface roughness for steel in ANSYS FLUENT was reasonable. In addition, the methodology could be further applied to future duty life studies for the cavitating venturi flow meter.

  14. Simulation of Flow Control Using Deformable Surfaces

    NASA Technical Reports Server (NTRS)

    Truman, C. Randall

    2001-01-01

    The goal of this investigation is to numerically simulate the effects of oscillatory actuators placed on the leading edge of an airfoil, and to quantify the effects of oscillatory blowing on an airfoil stall behavior. It has been demonstrated experimentally that periodic blowing can delay flow separation at high angle of attack. The computations are to be performed for a TAU 0015 airfoil at a high Reynolds number of approx. 1 x 10(exp 6) with turbulent flow conditions. The two-equation Wilcox k - w turbulence model has been shown to provide reliable descriptions of transition and turbulence at high Reynolds numbers. The results are to be compared to Seifert's experimental data.

  15. Active control of Boundary Layer Separation & Flow Distortion in Adverse Pressure Gradient Flows via Supersonic Microjets

    NASA Technical Reports Server (NTRS)

    Alvi, Farrukh S.; Gorton, Susan (Technical Monitor)

    2005-01-01

    Inlets to aircraft propulsion systems must supply flow to the compressor with minimal pressure loss, flow distortion or unsteadiness. Flow separation in internal flows such as inlets and ducts in aircraft propulsion systems and external flows such as over aircraft wings, is undesirable as it reduces the overall system performance. The aim of this research has been to understand the nature of separation and more importantly, to explore techniques to actively control this flow separation. In particular, the use of supersonic microjets as a means of controlling boundary layer separation was explored. The geometry used for the early part of this study was a simple diverging Stratford ramp, equipped with arrays of supersonic microjets. Initial results, based on the mean surface pressure distribution, surface flow visualization and Planar Laser Scattering (PLS) indicated a reverse flow region. We implemented supersonic microjets to control this separation and flow visualization results appeared to suggest that microjets have a favorable effect, at least to a certain extent. However, the details of the separated flow field were difficult to determine based on surface pressure distribution, surface flow patterns and PLS alone. It was also difficult to clearly determine the exact influence of the supersonic microjets on this flow. In the latter part of this study, the properties of this flow-field and the effect of supersonic microjets on its behavior were investigated in further detail using 2-component (planar) Particle Image Velocimetry (PIV). The results clearly show that the activation of microjets eliminated flow separation and resulted in a significant increase in the momentum of the fluid near the ramp surface. Also notable is the fact that the gain in momentum due to the elimination of flow separation is at least an order of magnitude larger (two orders of magnitude larger in most cases) than the momentum injected by the microjets and is accomplished with very

  16. Measurement and control of pressure driven flows in microfluidic devices using an optofluidic flow sensor

    PubMed Central

    Cheri, Mohammad Sadegh; Shahraki, Hamidreza; Sadeghi, Jalal; Moghaddam, Mohammadreza Salehi; Latifi, Hamid

    2014-01-01

    Measurement and control of pressure-driven flow (PDF) has a great potential to enhance the performance of chemical and biological experiments in Lab on a Chip technology. In this paper, we present an optofluidic flow sensor for real-time measurement and control of PDF. The optofluidic flow sensor consists of an on-chip micro Venturi and two optical Fabry-Pérot (FP) interferometers. Flow rate was measured from the fringe shift of FP interferometers resulted from movement fluid in the on-chip micro Venturi. The experimental results show that the optofluidic flow sensor has a minimum detectable flow change of 5 nl/min that is suitable for real time monitoring and control of fluids in many chemical and biological experiments. A Finite Element Method is used to solve the three dimensional (3D) Navier–Stokes and continuity equations to validate the experimental results. PMID:25584118

  17. Numerical Simulation of Fluidic Actuators for Flow Control Applications

    NASA Technical Reports Server (NTRS)

    Vasta, Veer N.; Koklu, Mehti; Wygnanski, Israel L.; Fares, Ehab

    2012-01-01

    Active flow control technology is finding increasing use in aerospace applications to control flow separation and improve aerodynamic performance. In this paper we examine the characteristics of a class of fluidic actuators that are being considered for active flow control applications for a variety of practical problems. Based on recent experimental work, such actuators have been found to be more efficient for controlling flow separation in terms of mass flow requirements compared to constant blowing and suction or even synthetic jet actuators. The fluidic actuators produce spanwise oscillating jets, and therefore are also known as sweeping jets. The frequency and spanwise sweeping extent depend on the geometric parameters and mass flow rate entering the actuators through the inlet section. The flow physics associated with these actuators is quite complex and not fully understood at this time. The unsteady flow generated by such actuators is simulated using the lattice Boltzmann based solver PowerFLOW R . Computed mean and standard deviation of velocity profiles generated by a family of fluidic actuators in quiescent air are compared with experimental data. Simulated results replicate the experimentally observed trends with parametric variation of geometry and inflow conditions.

  18. Double Stage Heat Transformer Controlled by Flow Ratio

    NASA Astrophysics Data System (ADS)

    Silva-Sotelo, S.; Romero, R. J.; Rodríguez – Martínez, A.

    this paper shows the values of Flow ratio (FR) for control of an absorption double stage heat transformer. The main parameters for the heat pump system are defined as COP, FR and GTL. The control of the entire system is based in a new definition of FR. The heat balance of the Double Stage Heat Transformer (DSHT) is used for the control. The mass flow is calculated for a HPVEE program and a second program control the mass flow. The mass flow is controlled by gear pumps connected to LabView program. The results show an increment in the fraction of the recovery energy. An example of oil distillation is used for the calculation. The waste heat energy is added at the system at 70 °C. Water ™ - Carrol mixture is used in the DSHT. The recover energy is obtained in a second absorber at 128 °C with two scenarios.

  19. Flow Separation Control Over a Ramp Using Sweeping Jet Actuators

    NASA Technical Reports Server (NTRS)

    Koklu, Mehti; Owens, Lewis R.

    2014-01-01

    Flow separation control on an adverse-pressure-gradient ramp model was investigated using various flow-control methods in the NASA Langley 15-Inch Wind Tunnel. The primary flow-control method studied used a sweeping jet actuator system to compare with more classic flow-control techniques such as micro-vortex generators, steady blowing, and steady- and unsteady-vortex generating jets. Surface pressure measurements and a new oilflow visualization technique were used to characterize the effects of these flow-control actuators. The sweeping jet actuators were run in three different modes to produce steady-straight, steady-angled, and unsteady-oscillating jets. It was observed that all of these flow-control methods are effective in controlling the separated flows on the ramp model. The steady-straight jet energizes the boundary layer by momentum addition and was found to be the least effective method for a fixed momentum coefficient. The steady-angled jets achieved better performance than the steady-straight jets because they generate streamwise vortices that energize the boundary layer by mixing high-momentum fluid with near wall low-momentum fluid. The unsteady-oscillating jets achieved the best performance by increasing the pressure recovery and reducing the downstream flow separation. Surface flow visualizations indicated that two out-of-phase counter-rotating vortices are generated per sweeping jet actuator, while one vortex is generated per vortex-generating jets. The extra vortex resulted in increased coverage, more pressure recovery, and reduced flow separation.

  20. Control of flow through a vapor generator

    DOEpatents

    Radcliff, Thomas D.

    2005-11-08

    In a Rankine cycle system wherein a vapor generator receives heat from exhaust gases, provision is made to avoid overheating of the refrigerant during ORC system shut down while at the same time preventing condensation of those gases within the vapor generator when its temperature drops below a threshold temperature by diverting the flow of hot gases to ambient and to thereby draw ambient air through the vapor generator in the process. In one embodiment, a bistable ejector is adjustable between one position, in which the hot gases flow through the vapor generator, to another position wherein the gases are diverted away from the vapor generator. Another embodiment provides for a fixed valve ejector with a bias towards discharging to ambient, but with a fan on the downstream side of said vapor generator for overcoming this bias.

  1. FoxA and LIPG endothelial lipase control the uptake of extracellular lipids for breast cancer growth

    PubMed Central

    Slebe, Felipe; Rojo, Federico; Vinaixa, Maria; García-Rocha, Mar; Testoni, Giorgia; Guiu, Marc; Planet, Evarist; Samino, Sara; Arenas, Enrique J.; Beltran, Antoni; Rovira, Ana; Lluch, Ana; Salvatella, Xavier; Yanes, Oscar; Albanell, Joan; Guinovart, Joan J.; Gomis, Roger R.

    2016-01-01

    The mechanisms that allow breast cancer (BCa) cells to metabolically sustain rapid growth are poorly understood. Here we report that BCa cells are dependent on a mechanism to supply precursors for intracellular lipid production derived from extracellular sources and that the endothelial lipase (LIPG) fulfils this function. LIPG expression allows the import of lipid precursors, thereby contributing to BCa proliferation. LIPG stands out as an essential component of the lipid metabolic adaptations that BCa cells, and not normal tissue, must undergo to support high proliferation rates. LIPG is ubiquitously and highly expressed under the control of FoxA1 or FoxA2 in all BCa subtypes. The downregulation of either LIPG or FoxA in transformed cells results in decreased proliferation and impaired synthesis of intracellular lipids. PMID:27045898

  2. FoxA and LIPG endothelial lipase control the uptake of extracellular lipids for breast cancer growth.

    PubMed

    Slebe, Felipe; Rojo, Federico; Vinaixa, Maria; García-Rocha, Mar; Testoni, Giorgia; Guiu, Marc; Planet, Evarist; Samino, Sara; Arenas, Enrique J; Beltran, Antoni; Rovira, Ana; Lluch, Ana; Salvatella, Xavier; Yanes, Oscar; Albanell, Joan; Guinovart, Joan J; Gomis, Roger R

    2016-01-01

    The mechanisms that allow breast cancer (BCa) cells to metabolically sustain rapid growth are poorly understood. Here we report that BCa cells are dependent on a mechanism to supply precursors for intracellular lipid production derived from extracellular sources and that the endothelial lipase (LIPG) fulfils this function. LIPG expression allows the import of lipid precursors, thereby contributing to BCa proliferation. LIPG stands out as an essential component of the lipid metabolic adaptations that BCa cells, and not normal tissue, must undergo to support high proliferation rates. LIPG is ubiquitously and highly expressed under the control of FoxA1 or FoxA2 in all BCa subtypes. The downregulation of either LIPG or FoxA in transformed cells results in decreased proliferation and impaired synthesis of intracellular lipids. PMID:27045898

  3. Acoustic responses of monodisperse lipid-encapsulated microbubble contrast agents produced by flow focusing

    PubMed Central

    Kaya, Mehmet; Feingold, Steven; Hettiarachchi, Kanaka; Lee, Abraham P; Dayton, Paul A

    2010-01-01

    Lipid-encapsulated microbubbles are used as contrast agents in ultrasound imaging. Currently available commercially made contrast agents have a polydisperse size distribution. It has been hypothesised that improved imaging sensitivity could be achieved with a uniform microbubble radius. We have recently developed microfluidics technology to produce contrast agents with a nearly monodisperse distribution. In this manuscript, we analyze echo responses from individual microbubbles from monodisperse populations in order to establish the relationship between scattered echo, microbubble radius, and excitation frequency. Simulations of bubble response from a modified Rayleigh-Plesset type model corroborate experimental data. Results indicate that microbubble echo response can be greatly increased by optimal combinations of microbubble radius and acoustic excitation frequency. These results may have a significant impact in the formulation of contrast agents to improve ultrasonic sensitivity. PMID:21475641

  4. Numerical Simulations of Plasma Based Flow Control Applications

    NASA Technical Reports Server (NTRS)

    Suzen, Y. B.; Huang, P. G.; Jacob, J. D.; Ashpis, D. E.

    2005-01-01

    A mathematical model was developed to simulate flow control applications using plasma actuators. The effects of the plasma actuators on the external flow are incorporated into Navier Stokes computations as a body force vector. In order to compute this body force vector, the model solves two additional equations: one for the electric field due to the applied AC voltage at the electrodes and the other for the charge density representing the ionized air. The model is calibrated against an experiment having plasma-driven flow in a quiescent environment and is then applied to simulate a low pressure turbine flow with large flow separation. The effects of the plasma actuator on control of flow separation are demonstrated numerically.

  5. Development and characterization of controlled release polar lipid microparticles of candesartan cilexetil by solid dispersion

    PubMed Central

    Kamalakkannan, V; Puratchikody, A; Ramanathan, L

    2013-01-01

    Candesartan cilexetil (CC) is a newer class of angiotensin II receptor antagonist used for the treatment of hypertension. The solubility of the CC is very poor and its oral bioavailability is only 15%. The controlledrelease polar lipid microparticles of CC (formulations F1, F2, F3 and F4) were prepared using variable erodible lipophilic excipients like hydrogenated castor oil, stearic acid, cetostearyl alcohol and carnauba wax by fusion method. The particle sizes of polar lipid microparticles were less than 50 microns and they were irregular in shape. Drug content ranged between 98.96 ± 2.1 and 101.9 ± 1.6% were present in all the formulations. The formulation F3 showed better drug release throughout the study period in a controlled release manner. Moreover, the in vitro release showed that all the formulations were best fitted to Higuchi model. Accelerated stability studies indicated that there was no significant changes in the chemical and physical characteristics of the formulated drug product during initial and at the end of the study period. The FTIR and DSC studies showed that there was no interaction between the drug and lipophilic excipients and no polymorphic transitions in all formulations. The X-ray diffraction peak of solid dispersion indicated that the crystalline nature of CC disappeared and no new peaks could be observed, suggesting the absence of interaction between drug and excipients. PMID:24019822

  6. Coupled parametric design of flow control and duct shape

    NASA Technical Reports Server (NTRS)

    Florea, Razvan (Inventor); Bertuccioli, Luca (Inventor)

    2009-01-01

    A method for designing gas turbine engine components using a coupled parametric analysis of part geometry and flow control is disclosed. Included are the steps of parametrically defining the geometry of the duct wall shape, parametrically defining one or more flow control actuators in the duct wall, measuring a plurality of performance parameters or metrics (e.g., flow characteristics) of the duct and comparing the results of the measurement with desired or target parameters, and selecting the optimal duct geometry and flow control for at least a portion of the duct, the selection process including evaluating the plurality of performance metrics in a pareto analysis. The use of this method in the design of inter-turbine transition ducts, serpentine ducts, inlets, diffusers, and similar components provides a design which reduces pressure losses and flow profile distortions.

  7. Control of Stall Flow over Airfoil using Vortex Generators

    NASA Astrophysics Data System (ADS)

    Hao, L. S.; Qiao, Z. D.; Song, W. P.

    2011-09-01

    In order to carry out the experimental investigation on control of stall flow over airfoil, two forms of the vortex generator layouts were designed. Comparison for the experimental data with and without vortex generators has been carried out, and the attention are focused on effects of stall flow over airfoil with different vortex generators layout. Experiment shows that the stall flow over airfoil is suppressed evidently by the first and second categories vortex generators, and the maximum lift coefficient is increased dramatically. The control of stall flow over airfoil with the second category vortex generator is much better than the first category vortex generator, and the smaller the inclined angle of the vortex generator is, the better the control effects of stall flow over airfoil will be.

  8. Photothermally controlled Marangoni flow around a micro bubble

    SciTech Connect

    Namura, Kyoko Nakajima, Kaoru; Kimura, Kenji; Suzuki, Motofumi

    2015-01-26

    We have experimentally investigated the control of Marangoni flow around a micro bubble using photothermal conversion. Using a focused laser spot acting as a highly localized heat source on Au nanoparticles/dielectric/Ag mirror thin film enables us to create a micro bubble and to control the temperature gradient around the bubble at a micrometer scale. When we irradiate the laser next to the bubble, a strong main flow towards the bubble and two symmetric rotation flows on either side of it develop. The shape of this rotation flow shows a significant transformation depending on the relative position of the bubble and the laser spot. Using this controllable rotation flow, we have demonstrated sorting of the polystyrene spheres with diameters of 2 μm and 0.75 μm according to their size.

  9. Racial Differences in Antilipemic Use and Lipid Control in High Risk Older Adults Post Medicare Part D

    PubMed Central

    Hanlon, Joseph T.; Boudreau, Robert M.; Perera, Subashan; Strotmeyer, Elsa S.; Newman, Anne B.; Simonsick, Eleanor M.; Shorr, Ronald I.; Bauer, Douglas C.; Donohue, Julie M.

    2013-01-01

    Background Older blacks are less likely to receive guideline-recommended antilipemic therapy and achieve lipid control than older whites due in part to out-of-pocket costs. We sought to determine whether racial differences in antilipemic use and lipid control narrowed after Medicare Part D’s implementation. Methods This before-after study included 1091 black and white adults age >70 with coronary heart disease and/or diabetes mellitus from the Health Aging and Body Composition Study. Primary outcomes were antilipemic use and LDL-C control. Key independent variables were race, time (pre- vs. post-Part D), and their interaction. Results Before Part D, fewer blacks than whites reported taking an antilipemic (32.70% vs 49.35%) and this difference was sustained after Part D (blacks 48.30% vs whites 64.57%). Multivariable generalized estimating equations confirmed no post Part D change in racial differences in antilipemic use (adjusted ratio of the odds ratios [AROR] 1.07, 95% CI 0.79–1.45). Compared to whites, more blacks had poor lipid control both before Part D (24.30% vs 12.36% respectively) and after Part D (24.46% vs 13.72% respectively), with no post Part D change in racial differences in lipid control (AROR 0.82, 95% CI 0.51–1.33). Conclusion While antilipemic use increased after Medicare Part D for both races, this policy change was associated neither with a change in lipid control for either racial group nor in the racial differences in antilipemic use or lipid control. PMID:24093862

  10. Effects of phylloquinone supplementation on lipid profile in women with rheumatoid arthritis: a double blind placebo controlled study

    PubMed Central

    Kolahi, Sousan; Pourghassem Gargari, Bahram; Mesgari Abbasi, Mehran; Asghari Jafarabadi, Mohammad

    2015-01-01

    BACKGROUND/OBJECTIVES Rheumatoid arthritis (RA) is associated with an excess mortality from cardiovascular disease which is likely attributed to an atherogenic lipid profile. Among nutritional factors vitamin K has been recently focused as a pivotal nutrient in improvement of lipid related markers. Thus, this study was designed to determine the effects of vitamin K on lipid profile in this disease. SUBJECTS/METHODS Fifty eight patients with definitive RA were participated in the present double blind placebo controlled study. They were randomly allocated into two groups to receive vitamin K1 as phylloquinone [10 mg/day] (n = 30) or placebo pills (n = 28), for eight weeks. In order to control the effects of probable confounders dietary intakes, anthropometric measurements including weight and height, clinical status using disease activity score-28 (DAS-28), physical activity and anxiety status were evaluated at baseline. Moreover, serum levels of lipid related markers including total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) were measured at baseline and at the end of intervention. RESULTS There were no significant differences between the two groups regarding any of the baseline characteristics. After adjusting for some relevant confounders, in comparison between two groups, we observed no significant changes in lipid related markers at the end of intervention. Also, there was no significant difference between before and after intervention values within groups (P > 0.05). CONCLUSIONS Function of vitamin K1 in lipid profile modification remains still controversial. This study showed that vitamin K1 has no effect on lipid profile in women with rheumatoid arthritis. Further studies with a longer follow-up are required to determine the effects of vitamin K on atherogenic lipid profile. PMID:25861426

  11. Radiant energy receiver having improved coolant flow control means

    DOEpatents

    Hinterberger, H.

    1980-10-29

    An improved coolant flow control for use in radiant energy receivers of the type having parallel flow paths is disclosed. A coolant performs as a temperature dependent valve means, increasing flow in the warmer flow paths of the receiver, and impeding flow in the cooler paths of the receiver. The coolant has a negative temperature coefficient of viscosity which is high enough such that only an insignificant flow through the receiver is experienced at the minimum operating temperature of the receiver, and such that a maximum flow is experienced at the maximum operating temperature of the receiver. The valving is accomplished by changes in viscosity of the coolant in response to the coolant being heated and cooled. No remotely operated valves, comparators or the like are needed.

  12. Measurement and control systems for an imaging electromagnetic flow metre.

    PubMed

    Zhao, Y Y; Lucas, G; Leeungculsatien, T

    2014-03-01

    Electromagnetic flow metres based on the principles of Faraday's laws of induction have been used successfully in many industries. The conventional electromagnetic flow metre can measure the mean liquid velocity in axisymmetric single phase flows. However, in order to achieve velocity profile measurements in single phase flows with non-uniform velocity profiles, a novel imaging electromagnetic flow metre (IEF) has been developed which is described in this paper. The novel electromagnetic flow metre which is based on the 'weight value' theory to reconstruct velocity profiles is interfaced with a 'Microrobotics VM1' microcontroller as a stand-alone unit. The work undertaken in the paper demonstrates that an imaging electromagnetic flow metre for liquid velocity profile measurement is an instrument that is highly suited for control via a microcontroller. PMID:24139307

  13. Simulation of inductive flow controlled by using Microplasma Actuator

    NASA Astrophysics Data System (ADS)

    Shimizu, Kazuo; Ito, Akihiko; Blajan, Marius; Yoneda, Hitoki

    2015-09-01

    Plasma actuator is a novel device for flow control because it has many advantages such as simple construction, no moving part, and quick response. In this study, microplasma actuator with four independent channels was used to generate upward and downward flow. The discharge gap was set at 25 μm, enabling the discharge to occur at the voltage of about 1 kV. Due to low discharge voltage the applied high-voltage could be controlled using FET switches easily. This enables to generate flexible flow. When a AC voltage of 1.4 kV and 20 kHz was applied, 0.6 m/s upward flow and 0.2 m/s downward flow were obtained. The numerical simulation using Suzen model was also carried out to investigate the flow velocity near the electrode surface since flow observation was difficult due to the reflected light from electrodes in PTV. In the simulation, we confirmed that the intensity of upward and downward flow was close to that in experiments. After applying a AC voltage for 2.5 ms, flow control was not finished, and considered to be the transient state. Vortices with the height of about 1.5 mm were occurred in both cases of experiments and the numerical simulations. On the other hand, after driving for 60 ms, the vortex development stopped and this stage was considered to be the steady state.

  14. Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions.

    PubMed

    Morita, Masamune; Onoe, Hiroaki; Yanagisawa, Miho; Ito, Hiroaki; Ichikawa, Masatoshi; Fujiwara, Kei; Saito, Hirohide; Takinoue, Masahiro

    2015-09-21

    We report a centrifugal microfluidic method, droplet-shooting and size-filtration (DSSF), for the production of cell-sized liposomes with controlled lipid compositions. This involves the generation of large and small droplets from the tip of a glass capillary and the selective transfer of small droplets through an oil-water interface, thus resulting in the generation of cell-sized liposomes. We demonstrate control of the microdomain formation as well as the formation of asymmetric lipid bilayer liposomes of uniform size by the control of lipid composition. The DSSF method involves simple microfluidics and is easy to use. In addition, only a small volume (0.5-2 μL) of sample solution is required for the formation of hundreds of cell-sized liposomes. We believe that this method can be applied to generate cell-sized liposomes for a wide variety of uses, such as the construction of artificial cell-like systems. PMID:26212462

  15. Automatic coolant flow control device for a nuclear reactor assembly

    DOEpatents

    Hutter, E.

    1984-01-27

    A device which controls coolant flow through a nuclear reactor assembly comprises a baffle means at the exit end of said assembly having a plurality of orifices, and a bimetallic member in operative relation to the baffle means such that at increased temperatures said bimetallic member deforms to unblock some of said orifices and allow increased coolant flow therethrough.

  16. Analysis of biomaterial latex-derived flow mechanical controller.

    PubMed

    Paula, Patricia M C; Rodrigues, Suelia S R; Brasil, Lourdes M; Silva, Rita C; da Rocha, Adson F

    2010-01-01

    This paper describes the basic guidelines for developing an innovative biomedical device. It covers the issues of researching about a suitable material, developing a new device, and testing its proprieties to check its effectiveness. The goal of the device is to control food flow into the esophagus, reducing its volume and the speed of food intake to help in the treatment of obesity. This module, called Esophageal Flow Controller (EFC®), is made of latex. Three different models of prototypes were developed, and 10 units of each model had their constructive and mechanical characteristics evaluated. All of them have followed the same manufacturing cycle. The results showed that the Esophageal Flow Control module has all the essential characteristics of an effective device for flow control in the esophagus. PMID:21096751

  17. RETROFITTING CONTROL FACILITIES FOR WET-WEATHER FLOW TREATMENT

    EPA Science Inventory

    Available technologies were evaluated to demonstrate the technical feasibility and cost effectiveness of retrofitting existing facilities to handle wet-weather flow. Cost/benefit relationships were also compared to construction of new conventional control and treatment facilities...

  18. RETROFITTING CONTROL FACILITIES FOR WET WEATHER FLOW TREATMENT

    EPA Science Inventory

    Available technologies were evaluated to demonstrate the technical feasibility and cost-effectiveness of retrofitting existing facilities to handle wet-weather flow. Cost/benefit relationships were also compared to construction of new conventional control and treatment facilitie...

  19. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

    SciTech Connect

    Cary, Robert E.

    2015-12-08

    Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

  20. Control of Population Flow in Coherently Driven Quantum Ladders

    SciTech Connect

    Garcia-Fernandez, Ruth; Bergmann, Klaas; Ekers, Aigars; Yatsenko, Leonid P.; Vitanov, Nikolay V.

    2005-07-22

    A technique for adiabatic control of the population flow through a preselected decaying excited level in a three-level quantum ladder is presented. The population flow through the intermediate or upper level is controlled efficiently and robustly by varying the pulse delay between a pair of partly overlapping coherent laser pulses. The technique is analyzed theoretically and demonstrated in an experiment with Na{sub 2} molecules.

  1. Flow control in a diffusing S-Duct

    NASA Technical Reports Server (NTRS)

    Vakili, A. D.; Wu, J. M.; Liver, P.; Bhat, M. K.

    1985-01-01

    Accurate measurements have been made of secondary flow in a 1.51 area ratio diffusing 30 deg - 30 deg S-Duct with circulair cross section. Turbulent flow was entering the duct at Mach number of 0.6, the boundary layer thickness at the duct entrance was ten percent of the duct inlet diameter. Through measurements made, local flow velocity vector as well as static and total pressures mapping of the flow at several stations were obtained. Strong secondary flow was measured in the first bend which continued into the second bend with new vorticity produced in there in the opposite direction. Surface oil flow visualization and wall pressures indicated a region of separated flow starting at theta approximately equal to 22 deg on the inside of the first bend up to theta approximately equal to 44 deg on the outside of the second bend. The flow separated in 'cyclone' form and never reattached in the duct. As a result of the secondary flow and the flow separation, significant total pressure distortion was observed at the exit of the duct. Using flow control devices the separation was eliminated while the exit distortion was improved.

  2. Research in Natural Laminar Flow and Laminar-Flow Control, part 2

    NASA Technical Reports Server (NTRS)

    Hefner, Jerry N. (Compiler); Sabo, Frances E. (Compiler)

    1987-01-01

    Part 2 of the Symposium proceedings includes papers addressing various topics in basic wind tunnel research/techniques and computational transitional research. Specific topics include: advanced measurement techniques; laminar flow control; Tollmien-Schlichting wave characteristics; boundary layer transition; flow visualization; wind tunnel tests; flight tests; boundary layer equations; swept wings; and skin friction.

  3. Demystifying ratings: How flow control shocks credit quality

    SciTech Connect

    Martin, J.H.

    1998-07-01

    Financial operations of many solid waste systems, waste-to-energy facilities in particular, have been shocked by the lack of congressional, and state and local legislation to resolve the loss of legal flow control. Flow control is a system's legal authority to direct waste into its own facilities. In contrast is economic flow control, where the market factors prevail and waste is brought to a facility based on competitive pricing. The loss of legal flow control threatens solid waste systems and impinges their underlying credit quality. Credit quality is expressed as the bond rating, a statement about the borrowers ability and willingness to repay debt in full and on time. While the courts have identified acceptable alternatives to enable municipal systems to diversity revenues (creating revenue flexibility), such alternatives may not be palatable as they represent additional taxation or fees. The paper highlights how the loss of legal flow control has shocked the operations, management and credit quality of solid waste systems. These shocks have stimulated public and private partnerships in order to facilitate economic flow control. Municipal credit solutions, credit impacts and credit trends are explained to identify how solid waste systems have responded in an operating climate exacerbated by regulatory changes (environmental and accounting) as well as utility deregulations. Analytical considerations are presented for evaluating the credit quality of solid waste bonds.

  4. An extended signal control strategy for urban network traffic flow

    NASA Astrophysics Data System (ADS)

    Yan, Fei; Tian, Fuli; Shi, Zhongke

    2016-03-01

    Traffic flow patterns are in general repeated on a daily or weekly basis. To improve the traffic conditions by using the inherent repeatability of traffic flow, a novel signal control strategy for urban networks was developed via iterative learning control (ILC) approach. Rigorous analysis shows that the proposed learning control method can guarantee the asymptotic convergence. The impacts of the ILC-based signal control strategy on the macroscopic fundamental diagram (MFD) were analyzed by simulations on a test road network. The results show that the proposed ILC strategy can evenly distribute the accumulation in the network and improve the network mobility.

  5. Nuclear reactor flow control method and apparatus

    DOEpatents

    Church, John P.

    1993-01-01

    Method and apparatus for improving coolant flow in a nuclear reactor during accident as well as nominal conditions. The reactor has a plurality of fuel elements in sleeves and a plenum above the fuel and through which the sleeves penetrate. Holes are provided in the sleeve so that coolant from the plenum can enter the sleeve and cool the fuel. The number and size of the holes are varied from sleeve to sleeve with the number and size of holes being greater for sleeves toward the center of the core and less for sleeves toward the periphery of the core. Preferably the holes are all the same diameter and arranged in rows and columns, the rows starting from the bottom of every sleeve and fewer rows in peripheral sleeves and more rows in the central sleeves.

  6. Nuclear reactor flow control method and apparatus

    DOEpatents

    Church, J.P.

    1993-03-30

    Method and apparatus for improving coolant flow in a nuclear reactor during accident as well as nominal conditions. The reactor has a plurality of fuel elements in sleeves and a plenum above the fuel and through which the sleeves penetrate. Holes are provided in the sleeve so that coolant from the plenum can enter the sleeve and cool the fuel. The number and size of the holes are varied from sleeve to sleeve with the number and size of holes being greater for sleeves toward the center of the core and less for sleeves toward the periphery of the core. Preferably the holes are all the same diameter and arranged in rows and columns, the rows starting from the bottom of every sleeve and fewer rows in peripheral sleeves and more rows in the central sleeves.

  7. Method, apparatus and system for controlling fluid flow

    DOEpatents

    McMurtrey, Ryan D.; Ginosar, Daniel M.; Burch, Joesph V.

    2007-10-30

    A system, apparatus and method of controlling the flow of a fluid are provided. In accordance with one embodiment of the present invention, a flow control device includes a valve having a flow path defined therethrough and a valve seat in communication with the flow path with a valve stem disposed in the valve seat. The valve stem and valve seat are cooperatively configured to cause mutual relative linear displacement thereof in response to rotation of the valve stem. A gear member is coupled with the rotary stem and a linear positioning member includes a portion which complementarily engages the gear member. Upon displacement of the linear positioning member along a first axis, the gear member and rotary valve stem are rotated about a second axis and the valve stem and valve seat are mutually linearly displaced to alter the flow of fluid through the valve.

  8. Controlling flow direction in nanochannels by electric field strength

    NASA Astrophysics Data System (ADS)

    Gao, Xiang; Zhao, Tianshou; Li, Zhigang

    2015-08-01

    Molecular dynamics simulations are conducted to study the flow behavior of CsF solutions in nanochannels under external electric fields E . It is found that the channel surface energy greatly affects the flow behavior. In channels of high surface energy, water molecules, on average, move in the same direction as that of the electric field regardless of the strength of E . In low surface energy channels, however, water transports in the opposite direction to the electric field at weak E and the flow direction is changed when E becomes sufficiently large. The direction change of water flow is attributed to the coupled effects of different water-ion interactions, inhomogeneous water viscosity, and ion distribution changes caused by the electric field. The flow direction change observed in this work may be employed for flow control in complex micro- or nanofluidic systems.

  9. Exhaust bypass flow control for exhaust heat recovery

    SciTech Connect

    Reynolds, Michael G.

    2015-09-22

    An exhaust system for an engine comprises an exhaust heat recovery apparatus configured to receive exhaust gas from the engine and comprises a first flow passage in fluid communication with the exhaust gas and a second flow passage in fluid communication with the exhaust gas. A heat exchanger/energy recovery unit is disposed in the second flow passage and has a working fluid circulating therethrough for exchange of heat from the exhaust gas to the working fluid. A control valve is disposed downstream of the first and the second flow passages in a low temperature region of the exhaust heat recovery apparatus to direct exhaust gas through the first flow passage or the second flow passage.

  10. Power Flow Controller for Renewables: Transformer-less Unified Power Flow Controller for Wind and Solar Power Transmission

    SciTech Connect

    2012-02-08

    GENI Project: MSU is developing a power flow controller to improve the routing of electricity from renewable sources through existing power lines. The fast, innovative, and lightweight circuitry that MSU is incorporating into its controller will eliminate the need for a separate heavy and expensive transformer, as well as the construction of new transmission lines. MSU’s controller is better suited to control power flows from distributed and intermittent wind and solar power systems than traditional transformer-based controllers are, so it will help to integrate more renewable energy into the grid. MSU‘s power flow controller can be installed anywhere in the existing grid to optimize energy transmission and help reduce transmission congestion.

  11. Laminar flow control, 1976 - 1982: A selected annotated bibliography

    NASA Technical Reports Server (NTRS)

    Tuttle, M. H.; Maddalon, D. V.

    1982-01-01

    Laminar Flow Control technology development has undergone tremendous progress in recent years as focused research efforts in materials, aerodynamics, systems, and structures have begun to pay off. A virtual explosion in the number of research papers published on this subject has occurred since interest was first stimulated by the 1976 introduction of NASA's Aircraft Energy Efficiency Laminar Flow Control Program. The purpose of this selected bibliography is to list available, unclassified laminar flow (both controlled and natural) research completed from about 1975 to mid 1982. Some earlier pertinent reports are included but listed separately in the Appendix. Reports listed herein emphasize aerodynamics and systems studies, but some structures work is also summarized. Aerodynamic work is mainly limited to the subsonic and transonic sped regimes. Because wind-tunnel flow qualities, such as free stream disturbance level, play such an important role in boundary-layer transition, much recent research has been done in this area and it is also included.

  12. Reduction of wind tunnel wall interference by controlled wall flow

    NASA Technical Reports Server (NTRS)

    Bernstein, S. (Editor); Joppa, R. G.

    1975-01-01

    An alternate method of testing was developed in which flow through the porous walls of the tunnel was actively controlled so as to approximate free air conditions in the neighborhood of the model during the test. The amount and distribution of the controlled flow through the walls is computed using a potential flow representation of the model based on the measured lift. Theoretical analysis is presented to prove the convergence of the method to free air conditions and to substantiate the general three-dimensional theory of operation when the normal flow distribution is continuous. A two-dimensional tunnel was constructed to evaluate the concept. Results show that substantial reduction of wall interference may be achieved with relatively low values of porosity of actively controlled walls.

  13. Compressor Performance Enhanced by Active Flow Control Over Stator Vanes

    NASA Technical Reports Server (NTRS)

    Culley, Dennis E.

    2003-01-01

    The application of active flow control technology to enhance turbomachinery system performance is being investigated at the NASA Glenn Research Center through experimental studies. Active flow control involves the use of sensors and actuators embedded within engine components to dynamically alter the internal flow path during off nominal operation in order to optimize engine performance and maintain stable operation. Modern compressors are already highly optimized components that must be designed to accommodate a broad range of operating conditions in a safe and efficient manner. Since overall engine performance is driven by compressor performance, advances in compressor technology that reduce weight and parts count, reduce fuel consumption, and lower maintenance costs will have a significant impact on the cost of aircraft ownership. Active flow control holds the promise of delivering such technology advances.

  14. Passive and Active Flow Control by Swimming Fishes and Mammals

    NASA Astrophysics Data System (ADS)

    Fish, F. E.; Lauder, G. V.

    2006-01-01

    What mechanisms of flow control do animals use to enhance hydrodynamic performance? Animals are capable of manipulating flow around the body and appendages both passively and actively. Passive mechanisms rely on structural and morphological components of the body (i.e., humpback whale tubercles, riblets). Active flow control mechanisms use appendage or body musculature to directly generate wake flow structures or stiffen fins against external hydrodynamic loads. Fish can actively control fin curvature, displacement, and area. The vortex wake shed by the tail differs between eel-like fishes and fishes with a discrete narrowing of the body in front of the tail, and three-dimensional effects may play a major role in determining wake structure in most fishes.

  15. Flow and Noise Control: Review and Assessment of Future Directions

    NASA Technical Reports Server (NTRS)

    Thomas, Russell H.; Choudhari, Meelan M.; Joslin, Ronald D.

    2002-01-01

    Technologies for developing radically new aerovehicles that would combine quantum leaps in cost, safety, and performance benefits with environmental friendliness have appeared on the horizon. This report provides both an assessment of the current state-of-the-art in flow and noise control and a vision for the potential gains to be made, in terms of performance benefit for civil and military aircraft and a unique potential for noise reduction, via future advances in flow and noise technologies. This report outlines specific areas of research that will enable the breakthroughs necessary to bring this vision to reality. Recent developments in many topics within flow and noise control are reviewed. The flow control overview provides succinct summaries of various approaches for drag reduction and improved maneuvering. Both exterior and interior noise problems are examined, including dominant noise sources, physics of noise generation and propagation, and both established and proposed concepts for noise reduction. Synergy between flow and noise control is a focus and, more broadly, the need to pursue research in a more concurrent approach involving multiple disciplines. Also discussed are emerging technologies such as nanotechnology that may have a significant impact on the progress of flow and noise control.

  16. Flow Characteristics Analysis of Widows' Creek Type Control Valve for Steam Turbine Control

    SciTech Connect

    Yoo, Yong H.; Sohn, Myoung S.; Suh, Kune Y.

    2006-07-01

    The steam turbine converts the kinetic energy of steam to mechanical energy of rotor blades in the power conversion system of fossil and nuclear power plants. The electric output from the generator of which the rotor is coupled with that of the steam turbine depends on the rotation velocity of the steam turbine bucket. The rotation velocity is proportional to the mass flow rate of steam entering the steam turbine through valves and nozzles. Thus, it is very important to control the steam mass flow rate for the load following operation of power plants. Among various valves that control the steam turbine, the control valve is most significant. The steam flow rate is determined by the area formed by the stem disk and the seat of the control valve. While the ideal control valve linearly controls the steam mass flow rate with its stem lift, the real control valve has various flow characteristic curves pursuant to the stem lift type. Thus, flow characteristic curves are needed to precisely design the control valves manufactured for the operating conditions of nuclear power plants. OMEGA (Optimized Multidimensional Experiment Geometric Apparatus) was built to experimentally study the flow characteristics of steam flowing inside the control valve. The Widows' Creek type control valve was selected for reference. Air was selected as the working fluid in the OMEGA loop to exclude the condensation effect in this simplified approach. Flow characteristic curves were plotted by calculating the ratio of the measured mass flow rate versus the theoretical mass flow rate of the air. The flow characteristic curves are expected to be utilized to accurately design and operate the control valve for fossil as well as nuclear plants. (authors)

  17. Assessing the In Vitro Drug Release from Lipid-Core Nanocapsules: a New Strategy Combining Dialysis Sac and a Continuous-Flow System.

    PubMed

    de Andrade, Diego Fontana; Zuglianello, Carine; Pohlmann, Adriana Raffin; Guterres, Silvia Stanisçuaski; Beck, Ruy Carlos Ruver

    2015-12-01

    The in vitro assessment of drug release from polymeric nanocapsules suspensions is one of the most studied parameters in the development of drug-loaded nanoparticles. Nevertheless, official methods for the evaluation of drug release from submicrometric carriers are not available. In this work, a new approach to assess the in vitro drug release profile from drug-loaded lipid-core nanocapsules (LNC) was proposed. A continuous-flow system (open system) was designed to evaluate the in vitro drug release profiles from different LNC formulations containing prednisolone or clobetasol propionate (LNC-CP) as drug model (LNC-PD) using a homemade apparatus. The release medium was constantly renewed throughout the experiment. A dialysis bag containing 5 mL of formulation (0.5 mg mL(-1)) was maintained inside the apparatus, under magnetic stirring and controlled temperature (37°C). In parallel, studies based on the conventional dialysis sac technique (closed system) were performed. It was possible to discriminate the in vitro drug release profile of different formulations using the open system. The proposed strategy improved the sink condition, by constantly renewing the release medium, thus maintaining the drug concentration farther from the saturated concentration in the release medium. Moreover, problems due to sampling errors can be easily overcome using this semi-automated system, since the collection is done automatically without interference from the analyst. The system proposed in this paper brings important methodological and analytical advantages, becoming a promising prototype semi-automated apparatus for performing in vitro drug release studies from drug-loaded lipid-core nanocapsules and other related nanoparticle drug delivery systems. PMID:25986595

  18. Lipid antigens in immunity

    PubMed Central

    Dowds, C. Marie; Kornell, Sabin-Christin

    2014-01-01

    Lipids are not only a central part of human metabolism but also play diverse and critical roles in the immune system. As such, they can act as ligands of lipid-activated nuclear receptors, control inflammatory signaling through bioactive lipids such as prostaglandins, leukotrienes, lipoxins, resolvins, and protectins, and modulate immunity as intracellular phospholipid- or sphingolipid-derived signaling mediators. In addition, lipids can serve as antigens and regulate immunity through the activation of lipid-reactive T cells, which is the topic of this review. We will provide an overview of the mechanisms of lipid antigen presentation, the biology of lipid-reactive T cells, and their contribution to immunity. PMID:23999493

  19. Active Flow Effectors for Noise and Separation Control

    NASA Technical Reports Server (NTRS)

    Turner, Travis L.

    2011-01-01

    New flow effector technology for separation control and enhanced mixing is based upon shape memory alloy hybrid composite (SMAHC) technology. The technology allows for variable shape control of aircraft structures through actively deformable surfaces. The flow effectors are made by embedding shape memory alloy actuator material in a composite structure. When thermally actuated, the flow effector def1ects into or out of the flow in a prescribed manner to enhance mixing or induce separation for a variety of applications, including aeroacoustic noise reduction, drag reduction, and f1ight control. The active flow effectors were developed for noise reduction as an alternative to fixed-configuration effectors, such as static chevrons, that cannot be optimized for airframe installation effects or variable operating conditions and cannot be retracted for off-design or fail-safe conditions. Benefits include: Increased vehicle control, overall efficiency, and reduced noise throughout all f1ight regimes, Reduced flow noise, Reduced drag, Simplicity of design and fabrication, Simplicity of control through direct current stimulation, autonomous re sponse to environmental heating, fast re sponse, and a high degree of geometric stability. The concept involves embedding prestrained SMA actuators on one side of the chevron neutral axis in order to generate a thermal moment and def1ect the structure out of plane when heated. The force developed in the host structure during def1ection and the aerodynamic load is used for returning the structure to the retracted position. The chevron design is highly scalable and versatile, and easily affords active and/or autonomous (environmental) control. The technology offers wide-ranging market applications, including aerospace, automotive, and any application that requires flow separation or noise control.

  20. Control of a Quadcopter Aerial Robot Using Optic Flow Sensing

    NASA Astrophysics Data System (ADS)

    Hurd, Michael Brandon

    This thesis focuses on the motion control of a custom-built quadcopter aerial robot using optic flow sensing. Optic flow sensing is a vision-based approach that can provide a robot the ability to fly in global positioning system (GPS) denied environments, such as indoor environments. In this work, optic flow sensors are used to stabilize the motion of quadcopter robot, where an optic flow algorithm is applied to provide odometry measurements to the quadcopter's central processing unit to monitor the flight heading. The optic-flow sensor and algorithm are capable of gathering and processing the images at 250 frames/sec, and the sensor package weighs 2.5 g and has a footprint of 6 cm2 in area. The odometry value from the optic flow sensor is then used a feedback information in a simple proportional-integral-derivative (PID) controller on the quadcopter. Experimental results are presented to demonstrate the effectiveness of using optic flow for controlling the motion of the quadcopter aerial robot. The technique presented herein can be applied to different types of aerial robotic systems or unmanned aerial vehicles (UAVs), as well as unmanned ground vehicles (UGV).

  1. Control and Automation of Fluid Flow, Mass Transfer and Chemical Reactions in Microscale Segmented Flow

    NASA Astrophysics Data System (ADS)

    Abolhasani, Milad

    Flowing trains of uniformly sized bubbles/droplets (i.e., segmented flows) and the associated mass transfer enhancement over their single-phase counterparts have been studied extensively during the past fifty years. Although the scaling behaviour of segmented flow formation is increasingly well understood, the predictive adjustment of the desired flow characteristics that influence the mixing and residence times, remains a challenge. Currently, a time consuming, slow and often inconsistent manual manipulation of experimental conditions is required to address this task. In my thesis, I have overcome the above-mentioned challenges and developed an experimental strategy that for the first time provided predictive control over segmented flows in a hands-off manner. A computer-controlled platform that consisted of a real-time image processing module within an integral controller, a silicon-based microreactor and automated fluid delivery technique was designed, implemented and validated. In a first part of my thesis I utilized this approach for the automated screening of physical mass transfer and solubility characteristics of carbon dioxide (CO2) in a physical solvent at a well-defined temperature and pressure and a throughput of 12 conditions per hour. Second, by applying the segmented flow approach to a recently discovered CO2 chemical absorbent, frustrated Lewis pairs (FLPs), I determined the thermodynamic characteristics of the CO2-FLP reaction. Finally, the segmented flow approach was employed for characterization and investigation of CO2-governed liquid-liquid phase separation process. The second part of my thesis utilized the segmented flow platform for the preparation and shape control of high quality colloidal nanomaterials (e.g., CdSe/CdS) via the automated control of residence times up to approximately 5 minutes. By introducing a novel oscillatory segmented flow concept, I was able to further extend the residence time limitation to 24 hours. A case study of a

  2. Passive control of supersonic asymmetric vortical flows around cones

    NASA Technical Reports Server (NTRS)

    Liu, C. H.; Kandil, Osama A.; Wong, Tin-Chee

    1992-01-01

    The unsteady, compressible, thin-layer Navier-Stokes equations are used to numerically study the passive control of steady and unsteady supersonic asymmetric flows around circular and noncircular cones. The main computational scheme of the present study is an implicit upwind, flux-difference splitting, finite-volume scheme. Passive control of flow asymmetry is studied by using a vertical fin in the leeward plane of geometric symmetry and side strakes with and without thickness at different orientations. The study focuses on circular-section cones since they are the most likely section-shapes for strong flow asymmetry. Side-strake passive control is shown to be more efficient and practical than vertical-fin passive control.

  3. Synthetic perspective optical flow: Influence on pilot control tasks

    NASA Technical Reports Server (NTRS)

    Bennett, C. Thomas; Johnson, Walter W.; Perrone, John A.; Phatak, Anil V.

    1989-01-01

    One approach used to better understand the impact of visual flow on control tasks has been to use synthetic perspective flow patterns. Such patterns are the result of apparent motion across a grid or random dot display. Unfortunately, the optical flow so generated is based on a subset of the flow information that exists in the real world. The danger is that the resulting optical motions may not generate the visual flow patterns useful for actual flight control. Researchers conducted a series of studies directed at understanding the characteristics of synthetic perspective flow that support various pilot tasks. In the first of these, they examined the control of altitude over various perspective grid textures (Johnson et al., 1987). Another set of studies was directed at studying the head tracking of targets moving in a 3-D coordinate system. These studies, parametric in nature, utilized both impoverished and complex virtual worlds represented by simple perspective grids at one extreme, and computer-generated terrain at the other. These studies are part of an applied visual research program directed at understanding the design principles required for the development of instruments displaying spatial orientation information. The experiments also highlight the need for modeling the impact of spatial displays on pilot control tasks.

  4. Superelevation and overspill control secondary flow dynamics in submarine channels

    NASA Astrophysics Data System (ADS)

    Dorrell, R. M.; Darby, S. E.; Peakall, J.; Sumner, E. J.; Parsons, D. R.; Wynn, R. B.

    2013-08-01

    In subaerial and submarine meander bends, fluid flow travels downstream in a helical spiral, the structure of which is determined by centrifugal, hydrostatic, baroclinic, and Coriolis forces that together balance frictional stresses generated by the flow. The sense of rotation of this helical flow, and in particular, whether the near bed flow is directed toward the inner bank, e.g., "river-normal," or outer bank, e.g., "river-reversed," is crucial to the morphodynamic evolution of the channel. However, in recent years, there has been a debate over the river-normal or river-reversed nature of submarine flows. Herein, we develop a novel three-dimensional closure of secondary flow dynamics, incorporating downstream convective material transport, to cast new light on this debate. Specifically, we show that the presence of net radial material transport, arising from flow superelevation and overspill, exerts a key control on the near bed orientation of secondary flow in submarine meanders. Our analysis implies that river-reversed flows are likely to be much more prevalent throughout submarine-canyon fan systems than prior studies have indicated.

  5. Passive flow control by membrane wings for aerodynamic benefit

    NASA Astrophysics Data System (ADS)

    Timpe, Amory; Zhang, Zheng; Hubner, James; Ukeiley, Lawrence

    2013-03-01

    The coupling of passive structural response of flexible membranes with the flow over them can significantly alter the aerodynamic characteristic of simple flat-plate wings. The use of flexible wings is common throughout biological flying systems inspiring many engineers to incorporate them into small engineering flying systems. In many of these systems, the motion of the membrane serves to passively alter the flow over the wing potentially resulting in an aerodynamic benefit. In this study, the aerodynamic loads and the flow field for a rigid flat-plate wing are compared to free trailing-edge membrane wings with two different pre-tensions at a chord-based Reynolds number of approximately 50,000. The membrane was silicon rubber with a scalloped free trailing edge. The analysis presented includes load measurements from a sting balance along with velocity fields and membrane deflections from synchronized, time-resolved particle image velocimetry and digital image correlation. The load measurements demonstrate increased aerodynamic efficiency and lift, while the synchronized flow and membrane measurements show how the membrane motion serves to force the flow. This passive flow control introduced by the membranes motion alters the flows development over the wing and into the wake region demonstrating how, at least for lower angles of attack, the membranes motion drives the flow as opposed to the flow driving the membrane motion.

  6. On the control of a canonical separated flow

    NASA Astrophysics Data System (ADS)

    Griffin, John C.

    Flow separation is generally an undesirable phenomenon that produces adverse effects to ideal aerodynamic performance. Control of ow separation is a complex problem and thus a popular area of research. A common obstacle is the lack of understanding of the complex fluid mechanics in cases of ow separation, evident by the substantial amount of ow control achieved through trial-and-error methods. The purpose of this work is to better understand the nature of separation for improved active control methods, which includes closed-loop control via reduced order methods. Control of a canonical separation problem, with the key features of separated flow, is achieved at a chord Reynolds number of 105. Separation is created on a at plate model, void of curvature that would otherwise include effects particular to the type of aerodynamic body. The characteristics of the imposed separation are evaluated with the intent of having a nominally two-dimensional separation, with the same essential flow characteristics of a more traditionally stalled airfoil. Results provide a reduced-order estimation technique that is used to identify global, dynamic modes through experimental measurements. Reattachment of the baseline separation is first achieved in open-loop control via ZNMF actuation. Efficient reattachment is reached by targeting the identified characteristic flow frequencies, which is able to reattach the separated flow with less than a quarter of the control effort as a comparison case with high-frequency forcing. The baseline and control results are used to identify a reduced-order model suitable for closed-loop control, with benefits of set-point tracking and full boundary layer attachment with minimum control effort.

  7. Triketocholanoic (Dehydrocholic) Acid. HEPATIC METABOLISM AND EFFECT ON BILE FLOW AND BILIARY LIPID SECRETION IN MAN

    PubMed Central

    Soloway, Roger D.; Hofmann, Alan F.; Thomas, Paul J.; Schoenfield, Leslie J.; Klein, Peter D.

    1973-01-01

    [24-14C]Dehydrocholic acid (triketo-5-β-cholanoic acid) was synthesized from [24-14C]cholic acid, mixed with 200 mg of carrier, and administered intravenously to two patients with indwelling T tubes designed to permit bile sampling without interruption of the enterohepatic circulation. More than 80% of infused radioactivity was excreted rapidly in bile as glycine- and taurine-conjugated bile acids. Radioactive products were identified, after deconjugation, as partially or completely reduced derivatives of dehydrocholic acid. By mass spectrometry, as well as chromatography, the major metabolite (about 70%) was a dihydroxy monoketo bile acid (3α,7α-dihydroxy-12-keto-5β-cholanoic acid); a second metabolite (about 20%) was a monohydroxy diketo acid (3α-hydroxy-7,12-di-keto-5β-cholanoic acid); and about 10% of radioactivity was present as cholic acid. Reduction appeared to have been sequential (3 position, then 7 position, and then 12 position) and stereospecific (only α epimers were recovered). Bile flow, expressed as the ratio of bile flow to bile acid excretion, was increased after dehydrocholic acid administration. It was speculated that the hydroxy keto metabolites are hydrocholeretics. The proportion of cholesterol to lecithin and bile acids did not change significantly after dehydrocholic acid administration. In vitro studies showed that the hydroxy keto metabolites dispersed lecithin poorly compared to cholate; however, mixtures of cholate and either metabolite had dispersant properties similar to those of cholate alone, provided the ratio of metabolite to cholate remained below a value characteristic for each metabolite. These experiments disclose a new metabolic pathway in man, provide further insight into the hydrocholeresis induced by keto bile acids, and indicate the striking change in pharmacologic and physical properties caused by replacement of hydroxyl by a keto substituent in the bile acid molecule. Images PMID:4685091

  8. Closed-loop Separation Control Using Oscillatory Flow Excitation

    NASA Technical Reports Server (NTRS)

    Allan, Brian G.; Juang, Jer-Nan; Raney, David L.; Seifert, Avi; Pack, latunia G.; Brown, Donald E.

    2000-01-01

    Design and implementation of a digital feedback controller for a flow control experiment was performed. The experiment was conducted in a cryogenic pressurized wind tunnel on a generic separated configuration at a chord Reynolds number of 16 million and a Mach number of 0.25. The model simulates the upper surface of a 20% thick airfoil at zero angle-of-attack. A moderate favorable pressure gradient, up to 55% of the chord, is followed by a severe adverse pressure gradient which is relaxed towards the trailing edge. The turbulent separation bubble, behind the adverse pressure gradient, is then reduced by introducing oscillatory flow excitation just upstream of the point of flow separation. The degree of reduction in the separation region can be controlled by the amplitude of the oscillatory excitation. A feedback controller was designed to track a given trajectory for the desired degree of flow reattachment and to improve the transient behavior of the flow system. Closed-loop experiments demonstrated that the feedback controller was able to track step input commands and improve the transient behavior of the open-loop response.

  9. Self-Contained Automated Methodology for Optimal Flow Control

    NASA Technical Reports Server (NTRS)

    Joslin, Ronald D.; Gunzburger, Max D.; Nicolaides, Roy A.; Erlebacherl, Gordon; Hussaini, M. Yousuff

    1997-01-01

    This paper describes a self-contained, automated methodology for active flow control which couples the time-dependent Navier-Stokes system with an adjoint Navier-Stokes system and optimality conditions from which optimal states, i.e., unsteady flow fields and controls (e.g., actuators), may be determined. The problem of boundary layer instability suppression through wave cancellation is used as the initial validation case to test the methodology. Here, the objective of control is to match the stress vector along a portion of the boundary to a given vector; instability suppression is achieved by choosing the given vector to be that of a steady base flow. Control is effected through the injection or suction of fluid through a single orifice on the boundary. The results demonstrate that instability suppression can be achieved without any a priori knowledge of the disturbance, which is significant because other control techniques have required some knowledge of the flow unsteadiness such as frequencies, instability type, etc. The present methodology has been extended to three dimensions and may potentially be applied to separation control, re-laminarization, and turbulence control applications using one to many sensors and actuators.

  10. Control of flow separation in a turbulent boundary layer

    NASA Astrophysics Data System (ADS)

    Cho, Minjeong; Choi, Sangho; Choi, Haecheon

    2015-11-01

    Towards the development of successful control methods for separation delay in a turbulent boundary layer, we adopt a model flow field, in which a turbulent separation occurs above a flat plate (Na and Moin 1998 JFM), and apply controls to this flow for reducing the size of the separation bubble and investigating the interaction between the forcing and flow near the separation bubble. We provide a single-frequency forcing with zero net mass flow rate at the upstream of the separation bubble. At low forcing frequencies, spanwise vortices are generated and travel downstream, bringing high momentum toward the wall and reducing the size of the separation bubble. Also, these vortices cause the separation and reattachment points to travel downstream. On the other hand, at high forcing frequencies, the size of the separation bubble becomes smaller and larger in time, respectively, due to the pressure gradient alternating favorably and adversely in time. Supported by NRF-2011-0028032 and 2014048162.

  11. Lockheed laminar-flow control systems development and applications

    NASA Technical Reports Server (NTRS)

    Lange, Roy H.

    1987-01-01

    Progress is summarized from 1974 to the present in the practical application of laminar-flow control (LFC) to subsonic transport aircraft. Those efforts included preliminary design system studies of commercial and military transports and experimental investigations leading to the development of the leading-edge flight test article installed on the NASA JetStar flight test aircraft. The benefits of LFC on drag, fuel efficiency, lift-to-drag ratio, and operating costs are compared with those for turbulent flow aircraft. The current activities in the NASA Industry Laminar-Flow Enabling Technologies Development contract include summaries of activities in the Task 1 development of a slotted-surface structural concept using advanced aluminum materials and the Task 2 preliminary conceptual design study of global-range military hybrid laminar flow control (HLFC) to obtain data at high Reynolds numbers and at Mach numbers representative of long-range subsonic transport aircraft operation.

  12. Combustor air flow control method for fuel cell apparatus

    DOEpatents

    Clingerman, Bruce J.; Mowery, Kenneth D.; Ripley, Eugene V.

    2001-01-01

    A method for controlling the heat output of a combustor in a fuel cell apparatus to a fuel processor where the combustor has dual air inlet streams including atmospheric air and fuel cell cathode effluent containing oxygen depleted air. In all operating modes, an enthalpy balance is provided by regulating the quantity of the air flow stream to the combustor to support fuel cell processor heat requirements. A control provides a quick fast forward change in an air valve orifice cross section in response to a calculated predetermined air flow, the molar constituents of the air stream to the combustor, the pressure drop across the air valve, and a look up table of the orifice cross sectional area and valve steps. A feedback loop fine tunes any error between the measured air flow to the combustor and the predetermined air flow.

  13. Spatial control of lipid droplet proteins by the ERAD ubiquitin ligase Doa10.

    PubMed

    Ruggiano, Annamaria; Mora, Gabriel; Buxó, Laura; Carvalho, Pedro

    2016-08-01

    The endoplasmic reticulum (ER) plays a central role in the biogenesis of most membrane proteins. Among these are proteins localized to the surface of lipid droplets (LDs), fat storage organelles delimited by a phospholipid monolayer. The LD monolayer is often continuous with the membrane of the ER allowing certain membrane proteins to diffuse between the two organelles. In these connected organelles, how some proteins concentrate specifically at the surface of LDs is not known. Here, we show that the ERAD ubiquitin ligase Doa10 controls the levels of some LD proteins. Their degradation is dependent on the localization to the ER and appears independent of the folding state. Moreover, we show that by degrading the ER pool of these LD proteins, ERAD contributes to restrict their localization to LDs. The signals for LD targeting and Doa10-mediated degradation overlap, indicating that these are competing events. This spatial control of protein localization is a novel function of ERAD that might contribute to generate functional diversity in a continuous membrane system. PMID:27357570

  14. Experimental Studies of Low-Pressure Turbine Flows and Flow Control

    NASA Technical Reports Server (NTRS)

    Volino, Ralph J.

    2012-01-01

    This report summarizes research performed in support of the NASA Glenn Research Center (GRC) Low-Pressure Turbine (LPT) Flow Physics Program. The work was performed experimentally at the U.S. Naval Academy faculties. The geometry corresponded to "Pak B" LPT airfoil. The test section simulated LPT flow in a passage. Three experimental studies were performed: (a) Boundary layer measurements for ten baseline cases under high and low freestream turbulence conditions at five Reynolds numbers of 25,000, 50,000, 100,000, 200,000, and 300,000, based on passage exit velocity and suction surface wetted length; (b) Passive flow control studies with three thicknesses of two-dimensional bars, and two heights of three-dimensional circular cylinders with different spanwise separations, at same flow conditions as the 10 baseline cases; (c) Active flow control with oscillating synthetic (zero net mass flow) vortex generator jets, for one case with low freestream turbulence and a low Reynolds number of 25,000. The Passive flow control was successful at controlling the separation problem at low Reynolds numbers, with varying degrees of success from case to case and varying levels of impact at higher Reynolds numbers. The active flow control successfully eliminated the large separation problem for the low Reynolds number case. Very detailed data was acquired using hot-wire anemometry, including single and two velocity components, integral boundary layer quantities, turbulence statistics and spectra, turbulent shear stresses and their spectra, and intermittency, documenting transition, separation and reattachment. Models were constructed to correlate the results. The report includes a summary of the work performed and reprints of the publications describing the various studies.

  15. Reduced-order Model Based Feedback Control of Cavity Flows -- Changes in the Flow Characteristics

    NASA Astrophysics Data System (ADS)

    Samimy, Mo; Little, Jesse; Debiasi, Marco; Caraballo, Edgar; Serrani, Andrea; Yuan, Xin

    2006-11-01

    We have developed and experimentally implemented reduced-order model based feedback control of subsonic cavity flows. Reduced-order models were developed via Proper Orthogonal Decomposition (POD) using particle imaging velocimetry (PIV) in conjunction with the Galerkin projection of the governing Navier-Stokes equations onto the resulting spatial eigenfunctions. The stochastic estimation technique using simultaneous PIV and surface pressure measurements was used to establish correlation between the flow field and surface pressure. For the implementation of the controller, dynamic surface pressure measurements were used for the estimation of the POD modal coefficients. The reduced-order model was linearized around the equilibrium point and a linear-quadratic optimal controller was designed and implemented in the experiments. The actuator was a compression driver type and its output was channeled through a one millimeter slit spanning the entire width of the cavity leading edge. Models based on one or more flow conditions for a Mach 0.3 cavity flow were developed and used, which suppressed the cavity resonant modes. We will compare and contrast the flow characteristics for the open- and closed-loop controlled flows.

  16. Light Control of the Flow of Phototactic Microswimmer Suspensions

    NASA Astrophysics Data System (ADS)

    Garcia, Xabel; Rafaï, Salima; Peyla, Philippe

    2013-03-01

    Some microalgae are sensitive to light intensity gradients. This property is known as phototaxis: The algae swim toward a light source (positive phototaxis). We use this property to control the motion of microalgae within a Poiseuille flow using light. The combination of flow vorticity and phototaxis results in a concentration of algae around the center of the flow. Intermittent light exposure allows analysis of the dynamics of this phenomenon and its reversibility. With this phenomenon, we hope to pave the way toward new algae concentration techniques (a bottleneck challenge in biofuel algal production) and toward the improvement of pollutant biodetector technology.

  17. Control of turbulent boundary layer flows by sound

    NASA Astrophysics Data System (ADS)

    Ahuja, K. K.; Whipkey, R. R.; Jones, G. S.

    1983-04-01

    The effects of acoustic excitation on the turbulent boundary-layer characteristics over an airfoil were examined as a function of excitation frequency and level and also flow velocity. The measured data primarily consisted of: (1) lift coefficients, (2) mean velocities and turbulence intensities as measured by a laser velocimeter, and (3) flow visualization. The experiments successfully demonstrated that separation of turbulent boundary layer flows can be controlled by sound in both pre- and post-stall regions. In addition, it was shown that, with high-frequency acoustic excitation, the turbulence levels in the boundary layer at a fixed measurement point can be reduced considerably.

  18. Flow monitoring and control system for injection wells

    DOEpatents

    Corey, John C.

    1993-01-01

    A system for monitoring and controlling the injection rate of fluid by an injection well of an in-situ remediation system for treating a contaminated groundwater plume. The well is fitted with a gated insert, substantially coaxial with the injection well. A plurality of openings, some or all of which are equipped with fluid flow sensors and gates, are spaced along the insert. The gates and sensors are connected to a surface controller. The insert may extend throughout part of, or substantially the entire length of the injection well. Alternatively, the insert may comprise one or more movable modules which can be positioned wherever desired along the well. The gates are opened part-way at the start of treatment. The sensors monitor and display the flow rate of fluid passing through each opening on a controller. As treatment continues, the gates are opened to increase flow in regions of lesser flow, and closed to decrease flow in regions of greater flow, thereby approximately equalizing the amount of fluid reaching each part of the plume.

  19. Flow monitoring and control system for injection wells

    DOEpatents

    Corey, J.C.

    1993-02-16

    A system for monitoring and controlling the injection rate of fluid by an injection well of an in-situ remediation system for treating a contaminated groundwater plume. The well is fitted with a gated insert, substantially coaxial with the injection well. A plurality of openings, some or all of which are equipped with fluid flow sensors and gates, are spaced along the insert. The gates and sensors are connected to a surface controller. The insert may extend throughout part of, or substantially the entire length of the injection well. Alternatively, the insert may comprise one or more movable modules which can be positioned wherever desired along the well. The gates are opened part-way at the start of treatment. The sensors monitor and display the flow rate of fluid passing through each opening on a controller. As treatment continues, the gates are opened to increase flow in regions of lesser flow, and closed to decrease flow in regions of greater flow, thereby approximately equalizing the amount of fluid reaching each part of the plume.

  20. Inlet Flow Control and Prediction Technologies for Embedded Propulsion Systems

    NASA Technical Reports Server (NTRS)

    McMillan, Michelle L.; Mackie, Scott A.; Gissen, Abe; Vukasinovic, Bojan; Lakebrink, Matthew T.; Glezer, Ari; Mani, Mori; Mace, James L.

    2011-01-01

    Fail-safe, hybrid, flow control (HFC) is a promising technology for meeting high-speed cruise efficiency, low-noise signature, and reduced fuel-burn goals for future, Hybrid-Wing-Body (HWB) aircraft with embedded engines. This report details the development of HFC technology that enables improved inlet performance in HWB vehicles with highly integrated inlets and embedded engines without adversely affecting vehicle performance. In addition, new test techniques for evaluating Boundary-Layer-Ingesting (BLI)-inlet flow-control technologies developed and demonstrated through this program are documented, including the ability to generate a BLI-like inlet-entrance flow in a direct-connect, wind-tunnel facility, as well as, the use of D-optimal, statistically designed experiments to optimize test efficiency and enable interpretation of results. Validated improvements in numerical analysis tools and methods accomplished through this program are also documented, including Reynolds-Averaged Navier-Stokes CFD simulations of steady-state flow physics for baseline, BLI-inlet diffuser flow, as well as, that created by flow-control devices. Finally, numerical methods were employed in a ground-breaking attempt to directly simulate dynamic distortion. The advances in inlet technologies and prediction tools will help to meet and exceed "N+2" project goals for future HWB aircraft.

  1. Active Flow Control on a Boundary-Layer-Ingesting Inlet

    NASA Technical Reports Server (NTRS)

    Gorton, Susan Althoff; Owens, Lewis R.; Jenkins, Luther N.; Allan, Brian G.; Schuster, Ernest P.

    2004-01-01

    Boundary layer ingestion (BLI) is explored as means to improve overall system performance for Blended Wing Body configuration. The benefits of BLI for vehicle system performance benefit are assessed with a process derived from first principles suitable for highly-integrated propulsion systems. This performance evaluation process provides framework within which to assess the benefits of an integrated BLI inlet and lays the groundwork for higher-fidelity systems studies. The results of the system study show that BLI provides a significant improvement in vehicle performance if the inlet distortion can be controlled, thus encouraging the pursuit of active flow control (AFC) as a BLI enabling technology. The effectiveness of active flow control in reducing engine inlet distortion was assessed using a 6% scale model of a 30% BLI offset, diffusing inlet. The experiment was conducted in the NASA Langley Basic Aerodynamics Research Tunnel with a model inlet designed specifically for this type of testing. High mass flow pulsing actuators provided the active flow control. Measurements were made of the onset boundary layer, the duct surface static pressures, and the mass flow through the duct and the actuators. The distortion was determined by 120 total pressure measurements located at the aerodynamic interface plane. The test matrix was limited to a maximum freestream Mach number of 0.15 with scaled mass flows through the inlet for that condition. The data show that the pulsed actuation can reduce distortion from 29% to 4.6% as measured by the circumferential distortion descriptor DC60 using less than 1% of inlet mass flow. Closed loop control of the actuation was also demonstrated using a sidewall surface static pressure as the response sensor.

  2. Pump and Flow Control Subassembly of Thermal Control Subsystem for Photovoltaic Power Module

    NASA Technical Reports Server (NTRS)

    Motil, Brian; Santen, Mark A.

    1993-01-01

    The pump and flow control subassembly (PFCS) is an orbital replacement unit (ORU) on the Space Station Freedom photovoltaic power module (PVM). The PFCS pumps liquid ammonia at a constant rate of approximately 1170 kg/hr while providing temperature control by flow regulation between the radiator and the bypass loop. Also, housed within the ORU is an accumulator to compensate for fluid volumetric changes as well as the electronics and firmware for monitoring and control of the photovoltaic thermal control system (PVTCS). Major electronic functions include signal conditioning, data interfacing and motor control. This paper will provide a description of each major component within the PFCS along with performance test data. In addition, this paper will discuss the flow control algorithm and describe how the nickel hydrogen batteries and associated power electronics will be thermally controlled through regulation of coolant flow to the radiator.

  3. Single DNA molecules on freestanding and supported cationic lipid bilayers: diverse conformational dynamics controlled by the local bilayer properties

    NASA Astrophysics Data System (ADS)

    Herold, Christoph; Schwille, Petra; Petrov, Eugene P.

    2016-02-01

    We present experimental results on the interaction of DNA macromolecules with cationic lipid membranes with different properties, including freestanding membranes in the fluid and gel state, and supported lipid membranes in the fluid state and under conditions of fluid-gel phase coexistence. We observe diverse conformational dynamics of membrane-bound DNA molecules controlled by the local properties of the lipid bilayer. In case of fluid-state freestanding lipid membranes, the behaviour of DNA on the membrane is controlled by the membrane charge density: whereas DNA bound to weakly charged membranes predominantly behaves as a 2D random coil, an increase in the membrane charge density leads to membrane-driven irreversible DNA collapse and formation of subresolution-sized DNA globules. On the other hand, electrostatic binding of DNA macromolecules to gel-state freestanding membranes leads to completely arrested diffusion and conformational dynamics of membrane-adsorbed DNA. A drastically different picture is observed in case of DNA interaction with supported cationic lipid bilayers: When the supported bilayer is in the fluid state, membrane-bound DNA molecules undergo 2D translational Brownian motion and conformational fluctuations, irrespectively of the charge density of the supported bilayer. At the same time, when the supported cationic membrane shows fluid-gel phase coexistence, membrane-bound DNA molecules are strongly attracted to micrometre-sized gel-phase domains enriched with the cationic lipid, which results in 2D compaction of the membrane-bound macromolecules. This DNA compaction, however, is fully reversible, and disappears as soon as the membrane is heated above the fluid-gel coexistence. We also discuss possible biological implications of our experimental findings.

  4. Flow control concepts for thread-based microfluidic devices

    PubMed Central

    Ballerini, David R.; Li, Xu; Shen, Wei

    2011-01-01

    The emerging concept of thread-based microfluidics has shown great promise for application to inexpensive disease detection and environmental monitoring. To allow the creation of more sophisticated and functional thread-based sensor designs, the ability to better control and understand the flow of fluids in the devices is required. To meet this end, various mechanisms for controlling the flow of reagents and samples in thread-based microfluidic devices are investigated in this study. A study of fluid penetration in single threads and in twined threads provides greater practical understanding of fluid velocity and ultimate penetration for the design of devices. “Switches” which control when or where flow can occur, or allow the mixing of multiple fluids, have been successfully prototyped from multifilament threads, plastic films, and household adhesive. This advancement allows the fabrication of more functional sensory devices which can incorporate more complex detection chemistries, while maintaining low production cost and simplicity of construction. PMID:21483659

  5. Network Adaptive Deadband: NCS Data Flow Control for Shared Networks

    PubMed Central

    Díaz-Cacho, Miguel; Delgado, Emma; Prieto, José A. G.; López, Joaquín

    2012-01-01

    This paper proposes a new middleware solution called Network Adaptive Deadband (NAD) for long time operation of Networked Control Systems (NCS) through the Internet or any shared network based on IP technology. The proposed middleware takes into account the network status and the NCS status, to improve the global system performance and to share more effectively the network by several NCS and sensor/actuator data flows. Relationship between network status and NCS status is solved with a TCP-friendly transport flow control protocol and the deadband concept, relating deadband value and transmission throughput. This creates a deadband-based flow control solution. Simulation and experiments in shared networks show that the implemented network adaptive deadband has better performance than an optimal constant deadband solution in the same circumstances. PMID:23208556

  6. Flow and Noise Control: Toward a Closer Linkage

    NASA Technical Reports Server (NTRS)

    Thomas, Russell H.; Choudhari, Meelan M.; Joslin, Ronald D.

    2002-01-01

    Motivated by growing demands for aircraft noise reduction and for revolutionary new aerovehicle concepts, the late twentieth century witnessed the beginning of a shift from single-discipline research, toward an increased emphasis on harnessing the potential of flow and noise control as implemented in a more fully integrated, multidisciplinary framework. At the same time, technologies for developing radically new aerovehicles, which promise quantum leap benefits in cost, safety and performance benefits with environmental friendliness, have appeared on the horizon. Transitioning new technologies to commercial applications will also require coupling further advances in traditional areas of aeronautics with intelligent exploitation of nontraditional and interdisciplinary technologies. Physics-based modeling and simulation are crucial enabling capabilities for synergistic linkage of flow and noise control. In these very fundamental ways, flow and noise control are being driven to be more closely linked during the early design phases of a vehicle concept for optimal and mutual noise and performance benefits.

  7. Mobile monolithic polymer elements for flow control in microfluidic devices

    DOEpatents

    Hasselbrink, Jr., Ernest F.; Rehm, Jason E.; Shepodd, Timothy J.

    2004-08-31

    A cast-in-place and lithographically shaped mobile, monolithic polymer element for fluid flow control in microfluidic devices and method of manufacture. Microfluid flow control devices, or microvalves that provide for control of fluid or ionic current flow can be made incorporating a cast-in-place, mobile monolithic polymer element, disposed within a microchannel, and driven by either fluid or gas pressure against a retaining or sealing surface. The polymer elements are made by the application of lithographic methods to monomer mixtures formulated in such a way that the polymer will not bond to microchannel walls. The polymer elements can seal against pressures greater than 5000 psi, and have a response time on the order of milliseconds. By the use of energetic radiation it is possible to depolymerize selected regions of the polymer element to form shapes that cannot be produced by conventional lithographic patterning and would be impossible to machine.

  8. Mobile Monolith Polymer Elements For Flow Control In Microfluidic Systems

    DOEpatents

    Hasselbrink, Jr., Ernest F.; Rehm, Jason E.; Shepodd, Timothy J.; Kirby, Brian J.

    2006-01-24

    A cast-in-place and lithographically shaped mobile, monolithic polymer element for fluid flow control in microfluidic devices and method of manufacture. Microfluid flow control devices, or microvalves that provide for control of fluid or ionic current flow can be made incorporating a cast-in-place, mobile monolithic polymer element, disposed within a microchannel, and driven by fluid pressure (either liquid or gas) against a retaining or sealing surface. The polymer elements are made by the application of lithographic methods to monomer mixtures formulated in such a way that the polymer will not bond to microchannel walls. The polymer elements can seal against pressures greater than 5000 psi, and have a response time on the order of milliseconds. By the use of energetic radiation it is possible to depolymerize selected regions of the polymer element to form shapes that cannot be produced by conventional lithographic patterning and would be impossible to machine.

  9. Mobile monolithic polymer elements for flow control in microfluidic devices

    DOEpatents

    Hasselbrink, Jr., Ernest F.; Rehm, Jason E.; Shepodd, Timothy J.; Kirby, Brian J.

    2005-11-11

    A cast-in-place and lithographically shaped mobile, monolithic polymer element for fluid flow control in microfluidic devices and method of manufacture. Microfluid flow control devices, or microvalves that provide for control of fluid or ionic current flow can be made incorporating a cast-in-place, mobile monolithic polymer element, disposed within a microchannel, and driven by fluid pressure (either liquid or gas) against a retaining or sealing surface. The polymer elements are made by the application of lithographic methods to monomer mixtures formulated in such a way that the polymer will not bond to microchannel walls. The polymer elements can seal against pressures greater than 5000 psi, and have a response time on the order of milliseconds. By the use of energetic radiation it is possible to depolymerize selected regions of the polymer element to form shapes that cannot be produced by conventional lithographic patterning and would be impossible to machine.

  10. Low-Speed Active Flow Control Laboratory Developed

    NASA Technical Reports Server (NTRS)

    Culley, Dennis E.; Bright, Michelle M.

    2005-01-01

    The future of aviation propulsion systems is increasingly focused on the application of control technologies to significantly enhance the performance of a new generation of air vehicles. Active flow control refers to a set of technologies that manipulate the flow of air and combustion gases deep within the confines of an engine to dynamically alter its performance during flight. By employing active flow control, designers can create engines that are significantly lighter, are more fuel efficient, and produce lower emissions. In addition, the operating range of an engine can be extended, yielding safer transportation systems. The realization of these future propulsion systems requires the collaborative development of many base technologies to achieve intelligent, embedded control at the engine locations where it will be most effective. NASA Glenn Research Center s Controls and Dynamics Technology Branch has developed a state-of-the-art low-speed Active Flow Control Laboratory in which emerging technologies can be integrated and explored in a flexible, low-cost environment. The facility allows the most promising developments to be prescreened and optimized before being tested on higher fidelity platforms, thereby reducing the cost of experimentation and improving research effectiveness.

  11. Numerical Laser Energy Deposition on Supersonic Cavity Flow and Sensor Placement Strategies to Control the Flow

    PubMed Central

    Aradag, Selin

    2013-01-01

    In this study, the impact of laser energy deposition on pressure oscillations and relative sound pressure levels (SPL) in an open supersonic cavity flow is investigated. Laser energy with a magnitude of 100 mJ is deposited on the flow just above the cavity leading edge and up to 7 dB of reduction is obtained in the SPL values along the cavity back wall. Additionally, proper orthogonal decomposition (POD) method is applied to the x-velocity data obtained as a result of computational fluid dynamics simulations of the flow with laser energy deposition. Laser is numerically modeled using a spherically symmetric temperature distribution. By using the POD results, the effects of laser energy on the flow mechanism are presented. A one-dimensional POD methodology is applied to the surface pressure data to obtain critical locations for the placement of sensors for real time flow control applications. PMID:24363612

  12. Numerical laser energy deposition on supersonic cavity flow and sensor placement strategies to control the flow.

    PubMed

    Yilmaz, Ibrahim; Aradag, Selin

    2013-01-01

    In this study, the impact of laser energy deposition on pressure oscillations and relative sound pressure levels (SPL) in an open supersonic cavity flow is investigated. Laser energy with a magnitude of 100 mJ is deposited on the flow just above the cavity leading edge and up to 7 dB of reduction is obtained in the SPL values along the cavity back wall. Additionally, proper orthogonal decomposition (POD) method is applied to the x-velocity data obtained as a result of computational fluid dynamics simulations of the flow with laser energy deposition. Laser is numerically modeled using a spherically symmetric temperature distribution. By using the POD results, the effects of laser energy on the flow mechanism are presented. A one-dimensional POD methodology is applied to the surface pressure data to obtain critical locations for the placement of sensors for real time flow control applications. PMID:24363612

  13. Unsteady aerodynamics and flow control for flapping wing flyers

    NASA Astrophysics Data System (ADS)

    Ho, Steven; Nassef, Hany; Pornsinsirirak, Nick; Tai, Yu-Chong; Ho, Chih-Ming

    2003-11-01

    The creation of micro air vehicles (MAVs) of the same general sizes and weight as natural fliers has spawned renewed interest in flapping wing flight. With a wingspan of approximately 15 cm and a flight speed of a few meters per second, MAVs experience the same low Reynolds number (10 4-10 5) flight conditions as their biological counterparts. In this flow regime, rigid fixed wings drop dramatically in aerodynamic performance while flexible flapping wings gain efficacy and are the preferred propulsion method for small natural fliers. Researchers have long realized that steady-state aerodynamics does not properly capture the physical phenomena or forces present in flapping flight at this scale. Hence, unsteady flow mechanisms must dominate this regime. Furthermore, due to the low flight speeds, any disturbance such as gusts or wind will dramatically change the aerodynamic conditions around the MAV. In response, a suitable feedback control system and actuation technology must be developed so that the wing can maintain its aerodynamic efficiency in this extremely dynamic situation; one where the unsteady separated flow field and wing structure are tightly coupled and interact nonlinearly. For instance, birds and bats control their flexible wings with muscle tissue to successfully deal with rapid changes in the flow environment. Drawing from their example, perhaps MAVs can use lightweight actuators in conjunction with adaptive feedback control to shape the wing and achieve active flow control. This article first reviews the scaling laws and unsteady flow regime constraining both biological and man-made fliers. Then a summary of vortex dominated unsteady aerodynamics follows. Next, aeroelastic coupling and its effect on lift and thrust are discussed. Afterwards, flow control strategies found in nature and devised by man to deal with separated flows are examined. Recent work is also presented in using microelectromechanical systems (MEMS) actuators and angular speed

  14. Research in Natural Laminar Flow and Laminar-Flow Control, part 3

    NASA Technical Reports Server (NTRS)

    Hefner, Jerry N. (Compiler); Sabo, Frances E. (Compiler)

    1987-01-01

    Part 3 of the Symposium proceedings contains papers addressing advanced airfoil development, flight research experiments, and supersonic transition/laminar flow control research. Specific topics include the design and testing of natural laminar flow (NLF) airfoils, NLF wing gloves, and NLF nacelles; laminar boundary-layer stability over fuselage forebodies; the design of low noise supersonic/hypersonic wind tunnels; and boundary layer instability mechanisms on swept leading edges at supersonic speeds.

  15. Effect of passive flow-control devices on turbulent low-speed base flow

    NASA Astrophysics Data System (ADS)

    Heidari-Miandoab, Farid

    Some configurations of blunt trailing-edge airfoils are known to have a lower pressure drag compared to sharp trailing-edge airfoils. However, this advantage in addition to the structural advantage of a thick trailing-edge airfoil is offset by its high base drag. At subsonic velocities, this is attributed to the low-pressure base flow dominated by a Karman vortex street. In the limiting case, the steady separated flow over a rearward-facing step is attained if the periodically shed vortices from a blunt trailing-edge are suppressed by the addition of a base spiltter-plate. Experimental studies in the Old Dominion University Low-Speed Closed-Circuit Wind Tunnel were conducted to examine the effect of several passive flow-control devices such as Wheeler doublets and wishbone vortex generators, longitudinal surface grooves, base cavities, and serrations on the characteristics of two- and three-dimensional base flows. Flow over flat-plate airfoil and rearward-facing step models was studied in the turbulent incompressible subsonic flow regime. Models with trailing-edge and step-sweep angles of 0, 30, and 45 degrees with respect to the crossflow direction were considered. Constant-temperature hot-wire anemometry, infrared surface thermography, and pitot-static probes were used to conduct flow measurements. The parameters measured included vortex shedding frequency, convective heat-transfer rates, base pressure, and flow reattachment distance. Surveys of mean velocity profiles in the wake were also conducted. Results have shown that most of the flow control devices tested increased the base pressure of the 2-D and 3-D flat-plate airfoils. Use of longitudinal surface grooves resulted in shorter flow reattachment distances and higher convective heat transfer rates downstream of the 2-D rearward-facing steps.

  16. Phase effect on flow control for dielectric barrier plasma actuators

    SciTech Connect

    Singh, K. P.; Roy, Subrata

    2006-07-03

    Active control of flow has a wide range of applications. Specifically, mitigation of detachment due to the weakly ionized gas flow past a flat plate at an angle of attack is studied using two asymmetric sets of electrode pairs kept at a phase lag. The equations governing the dynamics of electrons, helium ions, and neutrals are solved self-consistently with charge-Poisson equation. The electrodynamic forces produced by two actuators largely depend on the relative phase between the potentials applied to rf electrodes and distance between them. A suitable phase and an optimum distance exist between two actuators for effective separation control.

  17. Efficient Scheduling of Recursive Control Flow on GPUs

    SciTech Connect

    Huo, Xin; Krishnamoorthy, Sriram; Agrawal, Gagan

    2013-06-10

    Graphics processing units (GPUs) have rapidly emerged as a very significant player in high performance computing. Single instruction multiple thread (SIMT) pipelines are typically used in GPUs to exploit parallelism and maximize performance. Although support for unstructured control flow has been included in GPUs, efficiently managing thread divergence for arbitrary parallel programs remains a critical challenge. In this paper, we focus on the problem of supporting recursion in modern GPUs. We design and comparatively evaluate various algorithms to manage thread divergence encountered in recursive programs. The results improve upon traditional post-dominator based reconvergence mechanisms designed to handle thread divergence due to control flow within a procedure.

  18. Controlled polymorphic transformation of continuously crystallized solid lipid nanoparticles in a microstructured device: a feasibility study.

    PubMed

    Schoenitz, M; Joseph, S; Nitz, A; Bunjes, H; Scholl, S

    2014-04-01

    The contribution describes the transfer from a batch to a micro-continuous process for the production of stable solid lipid nanoparticles as drug carrier systems. Solid lipid nanoparticles are commonly prepared batch-wise often resulting in poorly defined product qualities with regard to the polymorphic state of their lipid matrix. In order to obtain solid lipid nanoparticle dispersions that meet the requirements for an acceptable pharmaceutical product, the manufacture of reproducible product qualities preferably containing the stable crystal form of the respective matrix lipid is necessary. These requests are addressed by the continuous preparation process of solid lipid nanoparticles. A four step feasibility study for the standardized evaluation whether or not a colloidal lipid dispersion is suitable for continuous crystallization of the particles resulting in stable crystal forms is presented. The process is based on the continuous crystallization and subsequent thermal treatment of differently stabilized, tripalmitin-based nanoparticle formulations in microstructured devices. The successful production of the stable crystal form by means of a continuous process chain is shown for a dispersion stabilized with a blend of hydrogenated soybean lecithin and sodium glycocholate. PMID:23969160

  19. Molecular recognition and controlled release in drug delivery systems based on nanostructured lipid surfactants

    NASA Astrophysics Data System (ADS)

    Angius, R.; Murgia, S.; Berti, D.; Baglioni, P.; Monduzzi, M.

    2006-08-01

    Several monoolein/water (MO/W) based liquid crystalline (LC) nanostructured mesophases have been revisited in view of the new trends of modern drug delivery formulations. The shape and amphiphilic character of the investigated lipid molecules address the preferential polar-apolar interfacial curvature and the delicate interplay of different intermolecular forces that drive self-assembly and thermodynamic stability of the nanostructures. Here some preliminary results related to the release of the antiviral drug 1-amine-adamantane hydrochloride, solubilized in the aqueous domain of bicontinuous cubic and reverse hexagonal LC phases, suggest these MO based LC phases as possible nano-depot systems for long term controlled release. Drug release was followed by conductivity measurements during a period of ten days. An effective and targeted drug delivery often requires a specific molecular recognition. With this aim, the possibility to entrap suitable molecules such as lauroylcholine (LCh, a cationic surfactant having a peptide-like polar head that can 'recognize' membrane proteins) and adenosine monophosphate disodium salt (NaAMP, an electrolyte that can 'recognize' purine receptors) has been tested. The addition of LCh to MO/W cubic gyroid (CG) LC phase causes a cubic-lamellar phase transition. The addition of NaAMP still allows the formation of the CG nanostructure. In the presence of both NaAMP and LCh again a CG LC phase forms. The bicontinuous CG LC phases have been characterized by NMR and SAXS.

  20. Some Thoughts on the Control of Bounded Shear Flows

    NASA Astrophysics Data System (ADS)

    Blackwelder, Ron F.

    1999-11-01

    The control of shear flows has been studied for years, but never as profusely as during the last decade of this century. In bounded shear flows, the control objective has often been to reduce the drag on the body in the flow field. This pursuit has produced many fascinating ideas, designs and body shapes. Devices and concepts used to reduce the pressure, viscous and induced drag on air and ground vehicles will be discussed. Examples will include the use of the Stratford pressure gradient which strives to achieve zero wall shear over a relaxing boundary layer. It has been used to develop high lift-to-drag airfoils and to reduce the drag on axisymmetrical bodies in aero- and hydro- applications. Other control methods have utilized actuators to alter the boundary conditions on different bodies. Small changes in local parameters on the surface have produced subtle modifications in the flow that have often led to large changes in the external flow field. The most successful usage of actuators has been to control the pressure drag of the body or the vorticity shed by it. More advanced methods have utilized sensors combined with actuators and a feed-forward control system to alter the flow field. Direct numerical simulations have suggested that these methods may offer large advantages in reducing the viscous drag on bodies. But can these or other methods reduce the viscous drag by 60-80%, as obtained with dilute long chain polymers? Unfortunately, the answer to that question remains elusive, but hopefully it will be answered early in the next century.

  1. Experimental study of controlled tip disturbance effect on flow asymmetry

    NASA Technical Reports Server (NTRS)

    Degani, David; Tobak, Murray

    1992-01-01

    The effect on the asymmetric mean flow observed on pointed bodies of revolution at incidence of changing the size and location of a controlled disturbance as well as changes in angle of attack and flow conditions are evaluated experimentally. Flow visualization and side-force measurements are carried out for a generic ogive-cylinder body inclined at high angle of attack in a low-speed wind tunnel. For all angles of attack tested (30-60 deg), minute changes in the size or location of the controlled disturbance result in finite changes in the asymmetric flow field, even to the extent of reversing the sign of the side force or becoming almost symmetric. The process is reversible; returning the wire to an original position likewise restores the corresponding flow field and mean side force. The variation of side force with continuous variation of a perturbation's size or location remains continuous and single valued, even in the incidence range of 50 to 60 deg, where 'bistable' behavior of the asymmetric flow field is observed.

  2. Formation of surface nanodroplets under controlled flow conditions

    PubMed Central

    Zhang, Xuehua; Lu, Ziyang; Tan, Huanshu; Bao, Lei; He, Yinghe; Sun, Chao; Lohse, Detlef

    2015-01-01

    Nanodroplets on a solid surface (i.e., surface nanodroplets) have practical implications for high-throughput chemical and biological analysis, lubrications, laboratory-on-chip devices, and near-field imaging techniques. Oil nanodroplets can be produced on a solid–liquid interface in a simple step of solvent exchange in which a good solvent of oil is displaced by a poor solvent. In this work, we experimentally and theoretically investigate the formation of nanodroplets by the solvent exchange process under well-controlled flow conditions. We find significant effects from the flow rate and the flow geometry on the droplet size. We develop a theoretical framework to account for these effects. The main idea is that the droplet nuclei are exposed to an oil oversaturation pulse during the exchange process. The analysis shows that the volume of the nanodroplets increases with the Peclet number Pe of the flow as ∝Pe3/4, which is in good agreement with our experimental results. In addition, at fixed flow rate and thus fixed Peclet number, larger and less homogeneously distributed droplets formed at less-narrow channels, due to convection effects originating from the density difference between the two solutions of the solvent exchange. The understanding from this work provides valuable guidelines for producing surface nanodroplets with desired sizes by controlling the flow conditions. PMID:26159418

  3. Overview of Active Flow Control at NASA Langley Research Center

    NASA Technical Reports Server (NTRS)

    Pack, L. G.; Joslin, R. D.

    1998-01-01

    The paper summarizes Active Flow Control projects currently underway at the NASA Langley Research Center. Technology development is being pursued within a multidisciplinary, cooperative approach, involving the classical disciplines of fluid mechanics, structural mechanics, material science, acoustics, and stability and control theory. Complementing the companion papers in this session, the present paper will focus on projects that have the goal of extending the state-of-the-art in the measurement, prediction, and control of unsteady, nonlinear aerodynamics. Toward this goal, innovative actuators, micro and macro sensors, and control strategies are considered for high payoff flow control applications. The target payoffs are outlined within each section below. Validation of the approaches range from bench-top experiments to wind-tunnel experiments to flight tests. Obtaining correlations for future actuator and sensor designs are implicit in the discussion. The products of the demonstration projects and design tool development from the fundamental NASA R&D level technology will then be transferred to the Applied Research components within NASA, DOD, and US Industry. Keywords: active flow control, separation control, MEMS, review

  4. Soy proteins and isoflavones reduce interleukin-6 but not serum lipids in older women: a randomized controlled trial.

    PubMed

    Mangano, Kelsey M; Hutchins-Wiese, Heather L; Kenny, Anne M; Walsh, Stephen J; Abourizk, Robin H; Bruno, Richard S; Lipcius, Rosanne; Fall, Pamela; Kleppinger, Alison; Kenyon-Pesce, Lisa; Prestwood, Karen M; Kerstetter, Jane E

    2013-12-01

    Soy foods contain several components, notably, isoflavones and amino acids, that may improve cardiovascular health. We evaluated the long-term effect of soy protein and/or soy isoflavones supplementation on serum lipids and inflammatory markers using a 1-year randomized, double-blind, placebo-control, clinical trial in 131 healthy ambulatory women older than 60 years. We hypothesized that soy protein, in combination with isoflavones, would have the largest positive effect on coronary heart disease risk factors (serum lipids and inflammatory markers) compared with either intervention alone and that, within groups receiving isoflavones, equol producers would have more positive effects on coronary heart disease risk factors than nonequol producers. After a 1-month baseline period, participants were randomized into 1 of 4 intervention groups: soy protein (18 g/d) and isoflavone tablets (105 mg/d isoflavone aglycone equivalents), soy protein and placebo tablets, control protein and isoflavone tablets, or control protein and placebo tablets. T Tests were used to assess differences between equol and nonequol producers. Ninety-seven women completed the trial. Consumption of protein powder and isoflavone tablets did not differ among groups, and compliance with study powder and tablets was 79% and 90%, respectively. After 1 year, in the entire population, there were either no or little effects on serum lipids and inflammatory markers, regardless of treatment group. Equol producers, when analyzed separately, had significant improvements in total cholesterol/high-density lipoprotein and low-density lipoprotein/high-density lipoprotein ratios (-5.9%, P = .02; -7.2%, P = .04 respectively). Soy protein and isoflavone (either alone or together) did not impact serum lipids or inflammatory markers. Therefore, they should not be considered an effective intervention to prevent cardiovascular disease because of lipid modification in healthy late postmenopausal women lacking the ability

  5. Phase Resolved Angular Velocity Control of Cross Flow Turbines

    NASA Astrophysics Data System (ADS)

    Strom, Benjamin; Brunton, Steven; Polagye, Brian

    2015-11-01

    Cross flow turbines have a number of operational advantages for the conversion of kinetic energy in marine or fluvial currents, but they are often less efficient than axial flow devices. Here a control scheme is presented in which the angular velocity of a cross flow turbine with two straight blades is prescribed as a function of azimuthal blade position, altering the time-varying effective angle of attack. Flume experiments conducted with a scale model turbine show approximately an 80% increase in turbine efficiency versus optimal constant angular velocity and constant resistive torque control schemes. Torque, drag, and lateral forces on one- and two-bladed turbines are analyzed and interpreted with bubble flow visualization to develop a simple model that describes the hydrodynamics responsible for the observed increase in mean efficiency. Challenges associated with implementing this control scheme on commercial-scale devices are discussed. If solutions are found, the performance increase presented here may impact the future development of cross flow turbines.

  6. Inlet Flow Control and Prediction Technologies for Embedded Propulsion Systems

    NASA Technical Reports Server (NTRS)

    McMillan, Michelle L.; Gissen, Abe; Vukasinovic, Bojan; Lakebrink, Matthew T.; Glezer, Ari; Mani, Mori; Mace, James

    2010-01-01

    Fail-safe inlet flow control may enable high-speed cruise efficiency, low noise signature, and reduced fuel-burn goals for hybrid wing-body aircraft. The objectives of this program are to develop flow control and prediction methodologies for boundary-layer ingesting (BLI) inlets used in these aircraft. This report covers the second of a three year program. The approach integrates experiments and numerical simulations. Both passive and active flow-control devices were tested in a small-scale wind tunnel. Hybrid actuation approaches, combining a passive microvane and active synthetic jet, were tested in various geometric arrangements. Detailed flow measurements were taken to provide insight into the flow physics. Results of the numerical simulations were correlated against experimental data. The sensitivity of results to grid resolution and turbulence models was examined. Aerodynamic benefits from microvanes and microramps were assessed when installed in an offset BLI inlet. Benefits were quantified in terms of recovery and distortion changes. Microvanes were more effective than microramps at improving recovery and distortion.

  7. System and method for bidirectional flow and controlling fluid flow in a conduit

    DOEpatents

    Ortiz, Marcos German

    1999-01-01

    A system for measuring bidirectional flow, including backflow, of fluid in a conduit. The system utilizes a structural mechanism to create a pressure differential in the conduit. Pressure sensors are positioned upstream from the mechanism, at the mechanism, and downstream from the mechanism. Data from the pressure sensors are transmitted to a microprocessor or computer, and pressure differential detected between the pressure sensors is then used to calculate the backflow. Control signals may then be generated by the microprocessor or computer to shut off valves located in the conduit, upon the occurrence of backflow, or to control flow, total material dispersed, etc. in the conduit.

  8. System and method for bidirectional flow and controlling fluid flow in a conduit

    DOEpatents

    Ortiz, M.G.

    1999-03-23

    A system for measuring bidirectional flow, including backflow, of fluid in a conduit is disclosed. The system utilizes a structural mechanism to create a pressure differential in the conduit. Pressure sensors are positioned upstream from the mechanism, at the mechanism, and downstream from the mechanism. Data from the pressure sensors are transmitted to a microprocessor or computer, and pressure differential detected between the pressure sensors is then used to calculate the backflow. Control signals may then be generated by the microprocessor or computer to shut off valves located in the conduit, upon the occurrence of backflow, or to control flow, total material dispersed, etc. in the conduit. 3 figs.

  9. Experimental Investigation of Flow Control in a Compact Inlet Duct

    NASA Astrophysics Data System (ADS)

    Debronsky, Brian; Amitay, Michael

    2012-11-01

    Attractive to aircraft designers are compact inlets, which implement curved flow paths from the air intake of the engine to the compressor face. A compromise must be made between the compactness of the inlet and its aerodynamic performance. The aerodynamic purpose of inlets is to decelerate the oncoming flow before reaching the engine while minimizing total pressure loss, unsteadiness and distortion. Low length-to-diameter ratio inlets have a high degree of curvature, which inevitably causes flow separation and secondary flows. To address this issue, active flow control was implemented on a compact (L/D = 1.6) inlet to improve its performance metrics. The experiments were conducted at a Mach number of 0.44, where the actuation from an array of skewed and pitched jets produced streamwise vortices opposite to the secondary flow structures. The actuation resulted in an improved pressure recovery at the aerodynamic interface plane (AIP), where both the strength of the secondary structures and the flow unsteadiness were significantly reduced. Northrop Grumman Corporation.

  10. Structural integrated sensor and actuator systems for active flow control

    NASA Astrophysics Data System (ADS)

    Behr, Christian; Schwerter, Martin; Leester-Schädel, Monika; Wierach, Peter; Dietzel, Andreas; Sinapius, Michael

    2016-04-01

    An adaptive flow separation control system is designed and implemented as an essential part of a novel high-lift device for future aircraft. The system consists of MEMS pressure sensors to determine the flow conditions and adaptive lips to regulate the mass flow and the velocity of a wall near stream over the internally blown Coanda flap. By the oscillating lip the mass flow in the blowing slot changes dynamically, consequently the momentum exchange of the boundary layer over a high lift flap required mass flow can be reduced. These new compact and highly integrated systems provide a real-time monitoring and manipulation of the flow conditions. In this context the integration of pressure sensors into flow sensing airfoils of composite material is investigated. Mechanical and electrical properties of the integrated sensors are investigated under mechanical loads during tensile tests. The sensors contain a reference pressure chamber isolated to the ambient by a deformable membrane with integrated piezoresistors connected as a Wheatstone bridge, which outputs voltage signals depending on the ambient pressure. The composite material in which the sensors are embedded consists of 22 individual layers of unidirectional glass fiber reinforced plastic (GFRP) prepreg. The results of the experiments are used for adapting the design of the sensors and the layout of the laminate to ensure an optimized flux of force in highly loaded structures primarily for future aeronautical applications. It can be shown that the pressure sensor withstands the embedding process into fiber composites with full functional capability and predictable behavior under stress.

  11. Passive Flow Separation Control Mechanism Inspired by Shark Skin

    NASA Astrophysics Data System (ADS)

    Oakley, India; Lang, Amy

    2015-11-01

    The following experimental work seeks to examine shark scales as passive flow-actuated separation control mechanisms. It is hypothesized that the actuation of these scales can in fact reduce pressure drag by inhibiting flow reversal and thereby prevent flow separation. In order to examine this mechanism at a fundamental level, three-dimensional sharkskin scales were simplified and modeled as two-dimensional flaps. To further simplify the experiment, the flaps were observed within a laminar boundary layer. The laminar boundary layer was grown over a long flat plate that was placed inside a water tunnel. A rotating cylinder was also used to induce an unsteady, increasing adverse pressure gradient, which generated a reversing flow. In order to visualize the potential actuation of the two-dimensional flaps DPIV (digital particle image velocimetry) was utilized. Three main objectives for this work included, the actuation of the two-dimensional flaps, the resistance to a reversed flow as a result of flap actuation and the prevention of flow separation. However once the experiment was conducted the flaps did not perform as previously hypothesized. The adverse pressure gradient induced by the rotating cylinder did not produce a reversing flow powerful enough to actuate the flaps. NSF REU Site Award 1358991.

  12. Active flow control on a 1:4 car model

    NASA Astrophysics Data System (ADS)

    Heinemann, Till; Springer, Matthias; Lienhart, Hermann; Kniesburges, Stefan; Othmer, Carsten; Becker, Stefan

    2014-05-01

    Lift and drag of a passenger car are strongly influenced by the flow field around its rear end. The bluff body geometry produces a detached, transient flow which induces fluctuating forces on the body, affecting the rear axle, which may distress dynamic stability and comfort significantly. The investigations presented here deal with a 1:4 scale model of a simplified test car geometry that produces fluctuating lift and drag due to its strongly rounded rear geometry. To examine the influence of active flow control on this behavior, steady air jets were realized to exhaust from thin slots across the rear in three different configurations. Investigations were performed at and included the capturing of effective integral lift and drag, velocity measurements in the surrounding flow field with Laser Doppler Anemometry, surface pressure measurements and surface oil flow visualization on the rear. The flow field was found to be dominated by two longitudinal vortices, developing from the detachment of the flow at the upper C-pillar positions, and a recirculating, transverse vortex above the rear window. With an air jet emerging from a slot across the surface right below the rear window section, tangentially directed upstream toward the roof section, total lift could be reduced by more than 7 %, with rear axle lift reduction of about 5 % and negligible drag affection (1 %).

  13. Controlled synthesis of poly(3-hexylthiophene) in continuous flow

    PubMed Central

    Seyler, Helga; Subbiah, Jegadesan; Jones, David John; Holmes, Andrew Bruce

    2013-01-01

    Summary There is an increasing demand for organic semiconducting materials with the emergence of organic electronic devices. In particular, large-area devices such as organic thin-film photovoltaics will require significant quantities of materials for device optimization, lifetime testing and commercialization. Sourcing large quantities of materials required for the optimization of large area devices is costly and often impossible to achieve. Continuous-flow synthesis enables straight-forward scale-up of materials compared to conventional batch reactions. In this study, poly(3-hexylthiophene), P3HT, was synthesized in a bench-top continuous-flow reactor. Precise control of the molecular weight was demonstrated for the first time in flow for conjugated polymers by accurate addition of catalyst to the monomer solution. The P3HT samples synthesized in flow showed comparable performance to commercial P3HT samples in bulk heterojunction solar cell devices. PMID:23946848

  14. Field-effect Flow Control in Polymer Microchannel Networks

    NASA Technical Reports Server (NTRS)

    Sniadecki, Nathan; Lee, Cheng S.; Beamesderfer, Mike; DeVoe, Don L.

    2003-01-01

    A new Bio-MEMS electroosmotic flow (EOF) modulator for plastic microchannel networks has been developed. The EOF modulator uses field-effect flow control (FEFC) to adjust the zeta potential at the Parylene C microchannel wall. By setting a differential EOF pumping rate in two of the three microchannels at a T-intersection with EOF modulators, the induced pressure at the intersection generated pumping in the third, field-free microchannel. The EOF modulators are able to change the magnitude and direction of the pressure pumping by inducing either a negative or positive pressure at the intersection. The flow velocity is tracked by neutralized fluorescent microbeads in the microchannels. The proof-of-concept of the EOF modulator described here may be applied to complex plastic ,microchannel networks where individual microchannel flow rates are addressable by localized induced-pressure pumping.

  15. Synthetic Hemozoin (β-Hematin) Crystals Nucleate at the Surface of Neutral Lipid Droplets that Control Their Sizes

    PubMed Central

    Ambele, Melvin A.; Sewell, B. Trevor; Cummings, Franscious R.; Smith, Peter J.; Egan, Timothy J.

    2013-01-01

    Emulsions of monopalmitoylglycerol (MPG) and of a neutral lipid blend (NLB), consisting of MPG, monostearoylglycerol, dipalmitoylglycerol, dioleoylglycerol and dilineoylglycerol (4:2:1:1:1), the composition associated with hemozoin from the malaria parasite Plasmodium falciparum, have been used to mediate the formation of β-hematin microcrystals. Transmission electron microscopy (TEM), electron diffraction and electron spectroscopic imaging/electron energy loss spectroscopy (ESI/EELS) have been used to characterize both the lipid emulsion and β-hematin crystals. The latter have been compared with β-hematin formed at a pentanol/aqueous interface and with hemozoin both within P. falciparum parasites and extracted from the parasites. When lipid and ferriprotoporphyrin IX solutions in 1:9 v/v acetone/methanol were thoroughly pre-mixed either using an extruder or ultrasound, β-hematin crystals were found formed in intimate association with the lipid droplets. These crystals resembled hemozoin crystals, with prominent {100} faces. Lattice fringes in TEM indicated that these faces made contact with the lipid surface. The average length of these crystals was 0.62 times the average diameter of NLB droplets and their size distributions were statistically equivalent after 10 min incubation, suggesting that the lipid droplets also controlled the sizes of the crystals. This most closely resembles hemozoin formation in the helminth worm Schistosoma mansoni, while in P. falciparum, crystal formation appears to be associated with the much more gently curved digestive vacuole membrane which apparently leads to formation of much larger hemozoin crystals, similar to those formed at the flat pentanol-water interface. PMID:24244110

  16. Verification of the karst flow model under laboratory controlled conditions

    NASA Astrophysics Data System (ADS)

    Gotovac, Hrvoje; Andric, Ivo; Malenica, Luka; Srzic, Veljko

    2016-04-01

    Karst aquifers are very important groundwater resources around the world as well as in coastal part of Croatia. They consist of extremely complex structure defining by slow and laminar porous medium and small fissures and usually fast turbulent conduits/karst channels. Except simple lumped hydrological models that ignore high karst heterogeneity, full hydraulic (distributive) models have been developed exclusively by conventional finite element and finite volume elements considering complete karst heterogeneity structure that improves our understanding of complex processes in karst. Groundwater flow modeling in complex karst aquifers are faced by many difficulties such as a lack of heterogeneity knowledge (especially conduits), resolution of different spatial/temporal scales, connectivity between matrix and conduits, setting of appropriate boundary conditions and many others. Particular problem of karst flow modeling is verification of distributive models under real aquifer conditions due to lack of above-mentioned information. Therefore, we will show here possibility to verify karst flow models under the laboratory controlled conditions. Special 3-D karst flow model (5.6*2.6*2 m) consists of concrete construction, rainfall platform, 74 piezometers, 2 reservoirs and other supply equipment. Model is filled by fine sand (3-D porous matrix) and drainage plastic pipes (1-D conduits). This model enables knowledge of full heterogeneity structure including position of different sand layers as well as conduits location and geometry. Moreover, we know geometry of conduits perforation that enable analysis of interaction between matrix and conduits. In addition, pressure and precipitation distribution and discharge flow rates from both phases can be measured very accurately. These possibilities are not present in real sites what this model makes much more useful for karst flow modeling. Many experiments were performed under different controlled conditions such as different

  17. Applications of boundary vorticity dynamics to flow simulation, airfoil design, and flow control

    NASA Astrophysics Data System (ADS)

    Zhu, Fanglin

    This dissertation is dedicated to the study of applications of the boundary vorticity dynamics theory. The vorticity generation mechanism from solid boundaries is studied first. The development of a two-dimensional viscous solver and an airfoil inverse design tool is conducted next. Finally, a new flow control method called "BVF cutting" is introduced and tested. Various source terms of the vorticity generation rate, i.e., boundary vorticity flux (BVF), are studied based on the tangential momentum balance on a solid surface. The direct connection between BVF and aerodynamic forces is rederived. The constraint of BVF imposed by the vorticity conservation is formulated and clarified. Two aerodynamic tools are developed in terms of BVF: A numerical Navier-Stokes (N-S) solver for two-dimensional incompressible steady or unsteady flows by using streamfunction-vorticity variables, with the BVF as the Neumann boundary condition; and an inverse design tool to develop airfoils with improved performance, in which the BVF is used as the objective function in the Euler limit of a viscous flow. An extensive validation of the N-S solver is conducted, and examples of improved airfoils are given mainly for the purpose of enhancing their lift coefficients and delaying the stall angles of attack. By reexamining the flow separation in terms of BVF, two possible ways to implement the BVF cutting method are examined, one of which is proved very effective. Enlightened by these, the most idealized objective for flow control is found. A principle for the perfect controlled flow and a guideline to judge the effectiveness of a flow control is proposed.

  18. Biomimetics and Tubercles on Flippers for Hydrodynamic Flow Control

    NASA Astrophysics Data System (ADS)

    Fish, Frank E.

    2011-11-01

    The biomimetic approach seeks to incorporate designs based on biological organisms into engineered technologies. Biomimetics can be used to engineer machines that emulate the performance of organisms, particularly in instances where the organism's performance exceeds current mechanical technology or provides new directions to solve existing problems. The ability to control the flow of water around the body dictates the performance of marine mammals in the aquatic environment. Morphological specializations of marine mammals afford mechanisms for passive flow control. Aside from the design of the body, which minimizes drag, the morphology of the appendages provide hydrodynamic advantages with respect to drag, lift, thrust, and stall. Of particular interest are the pectoral flippers of the humpback whale (Megaptera novaeangliae). These flippers act as wing-like structures to provide hydrodynamic lift for maneuvering. The use of any such wing-like structure in making small radius turns to enhance both agility and maneuverability is constrained by performance associated with stall. Delay of stall can be accomplished passively by modification of the flipper leading edge. The design of the flippers includes prominent leading edge bumps or tubercles. Such a design is exhibited by the leading edge tubercles on the flippers of humpback whales. These novel morphological structures induce a spanwise flow field of separated vortices alternating with regions of accelerated flow. The coupled flow regions maintain areas of attached flow and delay stall to high angles of attack. The morphological features of humpback whales for flow control can be utilized in the biomimetic design of engineered structures and commercial products for increased hydrodynamic performance. Nature retains a store of untouched knowledge, which would be beneficial in advancing technology.

  19. Flow characteristics of control valve for different strokes

    NASA Astrophysics Data System (ADS)

    Jablonská, Jana; Kozubková, Milada

    2016-03-01

    The article deals with the determination of flow characteristics and loss coefficients of control valve when the water flows in the interval of operating parameters, including the evaluation of vapour and air cavitation regime. The characteristics of the control valve are measured on the experimental equipment and subsequently loss coefficients are determined. Data from experimental measurements are used for creating of mathematical model with vapour and air cavitation and verification results. This validation will enable the application of methods of numerical modelling for valves of atypical dimensions e.g. for use in nuclear power industry. The correct knowledge of the valve characteristics and fundamental coefficients (e.g. flow coefficient, cavitation coefficient and loss coefficient) is necessarily required primarily for designers of pipe networks.

  20. ac power control in the Core Flow Test Loop

    SciTech Connect

    McDonald, D.W.

    1980-01-01

    This work represents a status report on a development effort to design an ac power controller for the Core Flow Test Loop. The Core Flow Test Loop will be an engineering test facility which will simulate the thermal environment of a gas-cooled fast-breeder reactor. The problems and limitations of using sinusoidal ac power to simulate the power generated within a nuclear reactor are addressed. The transformer-thyristor configuration chosen for the Core Flow Test Loop power supply is presented. The initial considerations, design, and analysis of a closed-loop controller prototype are detailed. The design is then analyzed for improved performance possibilities and failure modes are investigated at length. A summary of the work completed to date and a proposed outline for continued development completes the report.

  1. Hypersonic Flow Control Using Upstream Focused Energy Deposition

    NASA Technical Reports Server (NTRS)

    Riggins David W.; Nelson, H. F.

    1999-01-01

    A numerical study of centerline and off-centerline power deposition at a point upstream of a two-dimensional blunt body at Mach 6.5 at 30 km altitude are presented. The full Navier-Stokes equations are used. Wave drag, lift, and pitching moment are presented as a function of amount of power absorbed in the flow and absorption point location. It is shown that wave drag is considerably reduced. Modifications to the pressure distribution in the flow field due to the injected energy create lift and a pitching moment when the injection is off-centerline. This flow control concept may lead to effective ways to improve the performance and to stabilize and control hypersonic vehicles.

  2. Nanostructured lipid carrier-loaded hyaluronic acid microneedles for controlled dermal delivery of a lipophilic molecule.

    PubMed

    Lee, Sang Gon; Jeong, Jae Han; Lee, Kyung Min; Jeong, Kyu Ho; Yang, Huisuk; Kim, Miroo; Jung, Hyungil; Lee, Sangkil; Choi, Young Wook

    2014-01-01

    Nanostructured lipid carriers (NLCs) were employed to formulate a lipophilic drug into hydrophilic polymeric microneedles (MNs). Hyaluronic acid (HA) was selected as a hydrophilic and bioerodible polymer to fabricate MNs, and nile red (NR) was used as a model lipophilic molecule. NR-loaded NLCs were consolidated into the HA-based MNs to prepare NLC-loaded MNs (NLC-MNs). A dispersion of NLCs was prepared by high-pressure homogenization after dissolving NR in Labrafil and mixing with melted Compritol, resulting in 268 nm NLCs with a polydispersity index of 0.273. The NLC dispersion showed a controlled release of NR over 24 hours, following Hixson-Crowell's cube root law. After mixing the NLC dispersion with the HA solution, the drawing lithography method was used to fabricate NLC-MNs. The length, base diameter, and tip diameter of the NLC-MNs were approximately 350, 380, and 30 μm, respectively. Fluorescence microscopic imaging of the NLC-MNs helped confirm that the NR-loaded NLCs were distributed evenly throughout the MNs. In a skin permeation study performed using a Franz diffusion cell with minipig dorsal skin, approximately 70% of NR was localized in the skin after 24-hour application of NLC-MNs. Confocal laser scanning microscopy (z-series) of the skin at different depths showed strong fluorescence intensity in the epidermal layer, which appeared to spread out radially with the passage of time. This study indicated that incorporation of drug-loaded NLCs into MNs could represent a promising strategy for controlled dermal delivery of lipophilic drugs. PMID:24403833

  3. An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis.

    PubMed

    Yang, Fajun; Vought, Bryan W; Satterlee, John S; Walker, Amy K; Jim Sun, Z-Y; Watts, Jennifer L; DeBeaumont, Rosalie; Saito, R Mako; Hyberts, Sven G; Yang, Shaosong; Macol, Christine; Iyer, Lakshmanan; Tjian, Robert; van den Heuvel, Sander; Hart, Anne C; Wagner, Gerhard; Näär, Anders M

    2006-08-10

    The sterol regulatory element binding protein (SREBP) family of transcription activators are critical regulators of cholesterol and fatty acid homeostasis. We previously demonstrated that human SREBPs bind the CREB-binding protein (CBP)/p300 acetyltransferase KIX domain and recruit activator-recruited co-factor (ARC)/Mediator co-activator complexes through unknown mechanisms. Here we show that SREBPs use the evolutionarily conserved ARC105 (also called MED15) subunit to activate target genes. Structural analysis of the SREBP-binding domain in ARC105 by NMR revealed a three-helix bundle with marked similarity to the CBP/p300 KIX domain. In contrast to SREBPs, the CREB and c-Myb activators do not bind the ARC105 KIX domain, although they interact with the CBP KIX domain, revealing a surprising specificity among structurally related activator-binding domains. The Caenorhabditis elegans SREBP homologue SBP-1 promotes fatty acid homeostasis by regulating the expression of lipogenic enzymes. We found that, like SBP-1, the C. elegans ARC105 homologue MDT-15 is required for fatty acid homeostasis, and show that both SBP-1 and MDT-15 control transcription of genes governing desaturation of stearic acid to oleic acid. Notably, dietary addition of oleic acid significantly rescued various defects of nematodes targeted with RNA interference against sbp-1 and mdt-15, including impaired intestinal fat storage, infertility, decreased size and slow locomotion, suggesting that regulation of oleic acid levels represents a physiologically critical function of SBP-1 and MDT-15. Taken together, our findings demonstrate that ARC105 is a key effector of SREBP-dependent gene regulation and control of lipid homeostasis in metazoans. PMID:16799563

  4. Pneumatic vortical flow control at high angles of attack

    NASA Technical Reports Server (NTRS)

    Tavella, Domingo A.; Schiff, Lewis B.; Cummings, Russell M.

    1990-01-01

    The injection of thin, high-momentum jets of air into the fuselage forebody boundary layers of the F-18 aircraft is explored numerically as a means of controlling the onset of fuselage vortices and of generating yaw control forces. The study was carried out for an angle of attack of 30 deg with symmetrical and asymmetrical blowing configurations. One-sided blowing results in a strongly asymmetrical flow pattern in the fore portion of the fuselage, leading to a net lateral force.

  5. Flow manipulation and control methodologies for vacuum infusion processes

    NASA Astrophysics Data System (ADS)

    Alms, Justin B.

    Vacuum Infusion Processes (VIPs) are very attractive composite manufacturing processes since large structures such as fuselages and wind blades can be fabricated in a cost effective manner. In VIPs, the fabric layers are placed on a one sided mold which is closed by enveloping the entire mold with a thin plastic film and evacuating the air out. The vacuum compresses the fabric and when a resin inlet is opened, resin flows into the mold. The resin is allowed to cure before demolding the structure. However, VIPs causes non-repeatable and problematic resin filling patterns due to the heterogeneous nature of the material, nesting between various layers, and the hand labor utilized for laying up the fabric. The design of the manufacturing process routinely involves a trial and error model which make manufacturing costs and development time difficult to estimate. The clear solution to improving the reliability and robustness of VIPs is to implement a system capable of on-line flow control. While on-line flow control has been studied and developed for other composite manufacturing processes, the VIPs have been largely ignored as there are few process parameters that lend themselves to effective flow control. In this work, two new processes were discovered with the goal of on-line control of VIPs in mind. These two processes referred to as Flow Flooding Chamber (FFC) and Vacuum Induced Preform Relaxation (VIPR) will be discussed. They both employ an external vacuum chamber to influence the permeability of the fabric temporarily which allows one to redirect the resin flow to resin starved regions of the mold. The VIPR process in addition uses a low and regulated vacuum pressure in the external chamber to increase the permeability of the fabric in a controllable manner. The objective is to understand how the VIPR process affects the resin flow in order to implement it into a complete flow control and automated environment which will reduce or eliminate the variability

  6. ACHIEVING IRRIGATION RETURN FLOW QUALITY CONTROL THROUGH IMPROVED LEGAL SYSTEMS

    EPA Science Inventory

    The key to irrigated agricultural return flow quality control is proper utilization and management of the resource itself, and an accepted tool in out society is the law. This project is designed to develop legal alternatives that will facilitate the implementation of improved wa...

  7. Rotorcraft Fuselage Flow Control Using Plasma Streamwise Vortex Generators

    NASA Astrophysics Data System (ADS)

    Coleman, Dustin; Thomas, Flint

    2012-11-01

    Active flow control, in the form of dielectric barrier discharge (DBD) plasma actuators, is applied to a NASA ROBIN-mod7 generic rotorcraft fuselage model. The model is considered in what would be a typical cruise position i.e. a nose down position at α = -5° . This configuration gives rise to a massive 3-D flow separation over the aft ramp section of the fuselage, characterized by two counter-rotating, streamwise vortices. The control objective is to minimize these concentrated vortices by means of flush fuselage-mounted plasma streamwise vortex generators (PSVGs), and consequently, reduce the form drag of the vehicle. Experiments were conducted at freestream Mach and Reynolds numbers of M∞ = 0 . 12 and ReL = 2 . 65 million, respectively. Aerodynamic loads under both natural and controlled conditions were acquired through use of an ATI Mini40 6-component force sensor. The pressure field on the ramp section was monitored by a 128 count static pressure array. Likewise, the flow field was captured by time-resolved PIV wake surveys. Results are compared with previous studies that utilized active flow control by way of pulsed jets or combustion actuators. This work is supported under NASA Cooperative Agreement NNX10AM32G.

  8. Application of porous materials for laminar flow control

    NASA Technical Reports Server (NTRS)

    Pearce, W. E.

    1978-01-01

    Fairly smooth porous materials were elected for study Doweave; Fibermetal; Dynapore; and perforated titanium sheet. Factors examined include: surface smoothness; suction characteristics; porosity; surface impact resistance; and strain compatibility. A laminar flow control suction glove arrangement was identified with material combinations compatible with thermal expansion and structural strain.

  9. Control of flow separation and mixing by aerodynamic excitation

    NASA Technical Reports Server (NTRS)

    Rice, Edward J.; Abbott, John M.

    1990-01-01

    The recent research progress in the control of shear flows using unsteady aerodynamic excitation conducted at the NASA Lewis Research Center is reviewed. The program is of fundamental nature concentrating on the physics of the unsteady aerodynamic processes. This field of research is a fairly new development with great promise in the areas of enhanced mixing and flow separation control. Enhanced mixing research reported in this paper include influence of core turbulence, forced pairing of coherent structures, and saturation of mixing enhancement. Separation flow control studies included are for a two-dimensional diffuser, conical diffusers, and single airfoils. Ultimate applications of this research include aircraft engine inlet flow control at high angle of attack, wide angle diffusers, highly loaded airfoils as in turbomachinery, and ejector/suppressor nozzles for the supersonic transport. An argument involving the Coanda Effect is made here that all of the above mentioned application areas really only involve forms of shear layer mixing enhancement. The program also includes the development of practical excitation devices which might be used in aircraft applications.

  10. 93. TEMPERATURE AND FLOW RATE CONTROLS FOR SYSTEM 1 AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    93. TEMPERATURE AND FLOW RATE CONTROLS FOR SYSTEM 1 AND SYSTEM 2, FACING WEST IN MECHANICAL EQUIPMENT ROOM (101), LSB (BLDG. 770) - Vandenberg Air Force Base, Space Launch Complex 3, Launch Pad 3 West, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

  11. Control of flow separation and mixing by aerodynamic excitation

    NASA Technical Reports Server (NTRS)

    Rice, Edward J.; Abbott, John M.

    1990-01-01

    The recent research in the control of shear flows using unsteady aerodynamic excitation conducted at the NASA Lewis Research Center is reviewed. The program is of a fundamental nature, concentrating on the physics of the unsteady aerodynamic processes. This field of research is a fairly new development with great promise in the areas of enhanced mixing and flow separation control. Enhanced mixing research includes influence of core turbulence, forced pairing of coherent structures, and saturation of mixing enhancement. Separation flow control studies included are for a two-dimensional diffuser, conical diffusers, and single airfoils. Ultimate applications include aircraft engine inlet flow control at high angle of attack, wide angle diffusers, highly loaded airfoils as in turbomachinery, and ejector/suppressor nozzles for the supersonic transport. An argument involving the Coanda Effect is made that all of the above mentioned application areas really only involve forms of shear layer mixing enhancement. The program also includes the development of practical excitation devices which might be used in aircraft applications.

  12. Flow monitoring and control system for injection wells

    DOEpatents

    Corey, J.C.

    1991-01-01

    The present invention relates to a system for monitoring and controlling the rate of fluid flow from an injection well used for in-situ remediation of contaminated groundwater. The United States Government has rights in this invention pursuant to Contract No. DE-AC09-89SR18035 between the US Department of Energy and Westinghouse Savannah River Company.

  13. Rate-based ABR flow control using two timescale SPSA

    NASA Astrophysics Data System (ADS)

    Bhatnagar, Shalabh; Fu, Michael C.; Marcus, Steven I.

    1999-08-01

    In this paper, a two timescale simultaneous perturbation stochastic approximation algorithm is developed and applied to closed loop rate based available bit rate flow control. The relevant convergence results are stated and explained. Numerical experiments demonstrate fast convergence even in the presence of significant delays and a large number of parameterized policy levels.

  14. URBAN WET-WEATHER FLOW POLLUTION MANAGEMENT AND CONTROL

    EPA Science Inventory

    One of the challenges in protecting urban watersheds lies in effectively controlling the contaminants in both overland runoff and sewerage system overflows during wet-weather events. Abatement of wet-weather flow (WWF) pollution can be implemented at the source by land managemen...

  15. Experimental Feedback Control of Flow Induced Cavity Tones

    NASA Technical Reports Server (NTRS)

    Cabell, Randolph H.; Kegerise, Michael A.; Cox, David E.; Gibbs, Gary P.

    2005-01-01

    Discrete-time, linear quadratic methods were used to design feedback controllers for reducing tones generated by flow over a cavity. The dynamics of a synthetic jet actuator mounted at the leading edge of the cavity as observed by two microphones in the cavity were modeled over a broad frequency range using state space models computed from experimental data. Variations in closed loop performance as a function of model order, control order, control bandwidth, and state estimator design were studied using a cavity in the Probe Calibration Tunnel at NASA Langley. The controller successfully reduced the levels of multiple cavity tones at the tested flow speeds of Mach 0.275, 0.35, and 0.45. In some cases, the closed loop results were limited by excitation of sidebands of the cavity tones, or the creation of new tones at frequencies away from the cavity tones. Nonetheless, the results validate the combination of optimal control and experimentally-generated state space models, and suggest this approach may be useful for other flow control problems. The models were not able to account for non-linear dynamics, such as interactions between tones at different frequencies.

  16. Robust Feedback Control of Flow Induced Structural Radiation of Sound

    NASA Technical Reports Server (NTRS)

    Heatwole, Craig M.; Bernhard, Robert J.; Franchek, Matthew A.

    1997-01-01

    A significant component of the interior noise of aircraft and automobiles is a result of turbulent boundary layer excitation of the vehicular structure. In this work, active robust feedback control of the noise due to this non-predictable excitation is investigated. Both an analytical model and experimental investigations are used to determine the characteristics of the flow induced structural sound radiation problem. The problem is shown to be broadband in nature with large system uncertainties associated with the various operating conditions. Furthermore the delay associated with sound propagation is shown to restrict the use of microphone feedback. The state of the art control methodologies, IL synthesis and adaptive feedback control, are evaluated and shown to have limited success for solving this problem. A robust frequency domain controller design methodology is developed for the problem of sound radiated from turbulent flow driven plates. The control design methodology uses frequency domain sequential loop shaping techniques. System uncertainty, sound pressure level reduction performance, and actuator constraints are included in the design process. Using this design method, phase lag was added using non-minimum phase zeros such that the beneficial plant dynamics could be used. This general control approach has application to lightly damped vibration and sound radiation problems where there are high bandwidth control objectives requiring a low controller DC gain and controller order.

  17. Cidea Control of Lipid Storage and Secretion in Mouse and Human Sebaceous Glands

    PubMed Central

    Zhang, Shasha; Shui, Guanghou; Wang, Guanqun; Wang, Chao; Sun, Shuhong; Zouboulis, Christos C.; Xiao, Ran; Ye, Jing; Li, Wei

    2014-01-01

    Sebaceous glands are skin appendages that secrete sebum onto hair follicles to lubricate the hair and maintain skin homeostasis. In this study, we demonstrated that Cidea is expressed at high levels in lipid-laden mature sebocytes and that Cidea deficiency led to dry hair and hair loss in aged mice. In addition, Cidea-deficient mice had markedly reduced levels of skin surface lipids, including triacylglycerides (TAGs) and wax diesters (WDEs), and these mice were defective in water repulsion and thermoregulation. Furthermore, we observed that Cidea-deficient sebocytes accumulated a large number of smaller-sized lipid droplets (LDs), whereas overexpression of Cidea in human SZ95 sebocytes resulted in increased lipid storage and the accumulation of large LDs. Importantly, Cidea was highly expressed in human sebaceous glands, and its expression levels were positively correlated with human sebum secretion. Our data revealed that Cidea is a crucial regulator of sebaceous gland lipid storage and sebum lipid secretion in mammals and humans. PMID:24636991

  18. Research in natural laminar flow and laminar-flow control, part 1

    SciTech Connect

    Hefner, J.N.; Sabo, F.E.

    1987-12-01

    Since the mid 1970's, NASA, industry, and universities have worked together to conduct important research focused at developing laminar flow technology that could reduce fuel consumption for general aviation, commuter, and transport aircraft by as much as 40 to 50 percent. The symposium was planned in view of the recent accomplishments within the areas of laminar flow control and natural laminar flow, and the potential benefits of laminar flow technology to the civil and military aircraft communities in the United States. Included were technical sessions on advanced theory and design tool development; wind tunnel and flight research; transition measurement and detection techniques; low and high Reynolds number research; and subsonic and supersonic research.

  19. Research in Natural Laminar Flow and Laminar-Flow Control, part 1

    NASA Technical Reports Server (NTRS)

    Hefner, Jerry N. (Compiler); Sabo, Frances E. (Compiler)

    1987-01-01

    Since the mid 1970's, NASA, industry, and universities have worked together to conduct important research focused at developing laminar flow technology that could reduce fuel consumption for general aviation, commuter, and transport aircraft by as much as 40 to 50 percent. The symposium was planned in view of the recent accomplishments within the areas of laminar flow control and natural laminar flow, and the potential benefits of laminar flow technology to the civil and military aircraft communities in the United States. Included were technical sessions on advanced theory and design tool development; wind tunnel and flight research; transition measurement and detection techniques; low and high Reynolds number research; and subsonic and supersonic research.

  20. Variability of sap flow on forest hillslopes: patterns and controls

    NASA Astrophysics Data System (ADS)

    Hassler, Sibylle; Blume, Theresa

    2013-04-01

    Sap flow in trees is an essential variable in integrated studies of hydrologic fluxes. It gives indication of transpiration rates for single trees and, with a suitable method of upscaling, for whole stands. This information is relevant for hydrologic and climate models, especially for the prediction of change in water fluxes in the soil-plant-atmosphere continuum under climate change. To this end, we do not only need knowledge concerning the response of sapflow to atmospheric forcing but also an understanding of the main controls on its spatial variability. Our study site consists of several subcatchments of the Attert basin in Luxembourg underlain by schists of the Ardennes massif. Within these subcatchments we measure sap flow in more than 20 trees on a range of forested hillslopes covered by a variety of temperate deciduous tree species such as beech, oak, hornbeam and maple as well as conifers such as firs. Our sap flow sensors are based on the heat pulse velocity method and consist of three needles, one needle acting as the heating device and the other two holding three thermistors each, enabling us to simultaneously measure sap flow velocity at three different depths within the tree. In close proximity to the trees we collect additional data on soil moisture, matric potential and groundwater levels. First results show that the sensor design seems promising for an upscaling of the measured sap flow velocities to sap flow at the tree level. The maximum depth of actively used sapwood as well as the decrease in sap flow velocity with increasing depth in the tree can be determined by way of the three thermistors. Marked differences in sap flow velocity profiles are visible between the different species, resulting in differences in sap flow for trees of similar diameter. We examine the range of tree sap flow values and variation due to species, size class, slope position and exposition and finally relate them to the dynamics of soil moisture conditions with the

  1. Turbine Tip Clearance Active Flow Control using Plasma Actuators

    NASA Astrophysics Data System (ADS)

    Vanness, Daniel

    2005-11-01

    A low-speed linear cascade was used to examine the tip gap leakage flow and leakage vortex that exists within the low pressure turbine stage of a gas-turbine engine. The cascade array is composed of nine Pratt & Whitney ``PakB" blades, with the center blade having a variable tip gap up to five percent chord. Reynolds numbers based on axial chord varied from 10^4 to 10^5. Static pressure taps located at the midspan and near the tip of the blade were used to characterize the blade pressure distribution. A five-hole probe was also traversed in the downstream blade wake to ascertain velocity vectors and total pressure loss. Flow control in the form of a single-dielectric-barrier plasma actuator mounted on the blade tip was used to alter the leakage vortex by acting on the blade tip separation bubble, the blade tip shear layer instability, or the gap flow jet instability through the production of high frequency unsteady disturbances. The flow was documented through measurements with and without flow control for varying tip gaps and Reynolds numbers. The effect of the actuation on the tip leakage vortex and efficiency are investigated.

  2. Stabilising falling liquid film flows using feedback control

    NASA Astrophysics Data System (ADS)

    Thompson, Alice B.; Gomes, Susana N.; Pavliotis, Grigorios A.; Papageorgiou, Demetrios T.

    2016-01-01

    Falling liquid films become unstable due to inertial effects when the fluid layer is sufficiently thick or the slope sufficiently steep. This free surface flow of a single fluid layer has industrial applications including coating and heat transfer, which benefit from smooth and wavy interfaces, respectively. Here, we discuss how the dynamics of the system are altered by feedback controls based on observations of the interface height, and supplied to the system via the perpendicular injection and suction of fluid through the wall. In this study, we model the system using both Benney and weighted-residual models that account for the fluid injection through the wall. We find that feedback using injection and suction is a remarkably effective control mechanism: the controls can be used to drive the system towards arbitrary steady states and travelling waves, and the qualitative effects are independent of the details of the flow modelling. Furthermore, we show that the system can still be successfully controlled when the feedback is applied via a set of localised actuators and only a small number of system observations are available, and that this is possible using both static (where the controls are based on only the most recent set of observations) and dynamic (where the controls are based on an approximation of the system which evolves over time) control schemes. This study thus provides a solid theoretical foundation for future experimental realisations of the active feedback control of falling liquid films.

  3. Study and Control of Flow Past Disk, and Circular and Rectangular Cylinders Aligned in the Flow

    NASA Technical Reports Server (NTRS)

    Higuchi, Hiroshi

    2010-01-01

    Methodology similar to that used for our closed-loop control of Separation over NACA 0012 Airfoil (Pinier et al, AIAA Journal 2007) Synthetic Jet Actuators, Miniature Pressure Transducers Split POD with and without Actuations Flow state estimation from Pressure signature

  4. What mainly controls recession flows in river basins?

    NASA Astrophysics Data System (ADS)

    Biswal, Basudev; Nagesh Kumar, D.

    2014-03-01

    The ubiquity of the power law relationship between dQ/dt and Q for recession periods (-dQ/dt=kQα,Q being discharge at the basin outlet at time t) clearly hints at the existence of a dominant recession flow process that is common to all real basins. It is commonly assumed that a basin, during recession events, functions as a single phreatic aquifer resting on a impermeable horizontal bed or the Dupuit-Boussinesq (DB) aquifer, and with time different aquifer geometric conditions arise that give different values of α and k. The recently proposed alternative model, geomorphological recession flow model, however, suggests that recession flows are controlled primarily by the dynamics of the active drainage network (ADN). In this study we use data for several basins and compare the above two contrasting recession flow models in order to understand which of the above two factors dominates during recession periods in steep basins. Particularly, we do the comparison by selecting three key recession flow properties: (1) power law exponent α, (2) dynamic dQ/dt-Q relationship (characterized by k) and (3) recession timescale (time period for which a recession event lasts). Our observations suggest that neither drainage from phreatic aquifers nor evapotranspiration significantly controls recession flows. Results show that the value of α and recession timescale are not modeled well by DB aquifer model. However, the above mentioned three recession curve properties can be captured satisfactorily by considering the dynamics of the ADN as described by geomorphological recession flow model, possibly indicating that the ADN represents not just phreatic aquifers but the organization of various sub-surface storage systems within the basin.

  5. Experimental Investigation of Actuators for Flow Control in Inlet Ducts

    NASA Astrophysics Data System (ADS)

    Vaccaro, John; Elimelech, Yossef; Amitay, Michael

    2010-11-01

    Attractive to aircraft designers are compact inlets, which implement curved flow paths to the compressor face. These curved flow paths could be employed for multiple reasons. One of which is to connect the air intake to the engine embedded in the aircraft body. A compromise must be made between the compactness of the inlet and its aerodynamic performance. The aerodynamic purpose of inlets is to decelerate the oncoming flow before reaching the engine while minimizing total pressure loss, unsteadiness and distortion. Low length-to-diameter ratio inlets have a high degree of curvature, which inevitably causes flow separation and secondary flows. Currently, the length of the propulsion system is constraining the overall size of Unmanned Air Vehicles (UAVs), thus, smaller more efficient aircrafts could be realized if the propulsion system could be shortened. Therefore, active flow control is studied in a compact (L/D=1.5) inlet to improve performance metrics. Actuation from a spanwise varying coanda type ejector actuator and a hybrid coanda type ejector / vortex generator jet actuator is investigated. Special attention will be given to the pressure recovery at the AIP along with unsteady pressure signatures along the inlet surface and at the AIP.

  6. Optopharmacological control of TRPC channels by coumarin-caged lipids is associated with a phototoxic membrane effect.

    PubMed

    Tiapko, Oleksandra; Bacsa, Bernadett; de la Cruz, Gema Guedes; Glasnov, Toma; Groschner, Klaus

    2016-08-01

    Photouncaging of second messengers has been successfully employed to gain mechanistic insight of cellular signaling pathways. One of the most enigmatic processes of ion channel regulation is lipid recognition and lipid-gating of TRPC channels, which represents pivotal mechanisms of cellular Ca(2+) homeostasis. Recently, optopharmacological tools including caged lipid mediators became available, enabling an unprecedented level of temporal and spatial control of the activating lipid species within a cellular environment. Here we tested a commonly used caged ligand approach for suitability to investigate TRPC signaling at the level of membrane conductance and cellular Ca(2+) handling. We report a specific photouncaging artifact that is triggered by the cage structure coumarin at UV illumination. Electrophysiological characterization identified a light-dependent membrane effect of coumarin. UV light (340 nm) as used for photouncaging, initiated a membrane conductance specifically in the presence of coumarin as low as 30 μmol L(-1) concentrations. This conductance masked the TRPC3 conductance evoked by photouncaging, while TRPC-mediated cellular Ca(2+) responses were largely preserved. The observed light-induced membrane effects of the released caging moiety may well interfere with certain cellular functions, and prompt caution in using coumarin-caged second messengers in cellular studies. PMID:27430887

  7. Unsteady transonic flow control around an airfoil in a channel

    NASA Astrophysics Data System (ADS)

    Hamid, Md. Abdul; Hasan, A. B. M. Toufique; Ali, Mohammad; Mitsutake, Yuichi; Setoguchi, Toshiaki; Yu, Shen

    2016-04-01

    Transonic internal flow around an airfoil is associated with self-excited unsteady shock wave oscillation. This unsteady phenomenon generates buffet, high speed impulsive noise, non-synchronous vibration, high cycle fatigue failure and so on. Present study investigates the effectiveness of perforated cavity to control this unsteady flow field. The cavity has been incorporated on the airfoil surface. The degree of perforation of the cavity is kept constant as 30%. However, the number of openings (perforation) at the cavity upper wall has been varied. Results showed that this passive control reduces the strength of shock wave compared to that of baseline airfoil. As a result, the intensity of shock wave/boundary layer interaction and the root mean square (RMS) of pressure oscillation around the airfoil have been reduced with the control method.

  8. Application of laminar flow control to supersonic transport configurations

    NASA Technical Reports Server (NTRS)

    Parikh, P. G.; Nagel, A. L.

    1990-01-01

    The feasibility and impact of implementing a laminar flow control system on a supersonic transport configuration were investigated. A hybrid laminar flow control scheme consisting of suction controlled and natural laminar flow was developed for a double-delta type wing planform. The required suction flow rates were determined from boundary layer stability analyses using representative wing pressure distributions. A preliminary design of structural modifications needed to accommodate suction through a perforated titanium skin was carried out together with the ducting and systems needed to collect, compress and discharge the suction air. The benefits of reduced aerodynamic drag were weighed against the weight, volume and power requirement penalties of suction system installation in a mission performance and sizing program to assess the net benefits. The study showed a feasibility of achieving significant laminarization of the wing surface by use of a hybrid scheme, leading to an 8.2 percent reduction in the cruise drag. This resulted in an 8.5 percent reduction in the maximum takeoff weight and a 12 percent reduction in the fuel burn after the inclusion of the LFC system installation penalties. Several research needs were identified for a resolution of aerodynamics, structural and systems issues before these potential benefits could be realized in a practical system.

  9. Active Flow Control: Instrumentation Automation and Experimental Technique

    NASA Technical Reports Server (NTRS)

    Gimbert, N. Wes

    1995-01-01

    In investigating the potential of a new actuator for use in an active flow control system, several objectives had to be accomplished, the largest of which was the experimental setup. The work was conducted at the NASA Langley 20x28 Shear Flow Control Tunnel. The actuator named Thunder, is a high deflection piezo device recently developed at Langley Research Center. This research involved setting up the instrumentation, the lighting, the smoke, and the recording devices. The instrumentation was automated by means of a Power Macintosh running LabVIEW, a graphical instrumentation package developed by National Instruments. Routines were written to allow the tunnel conditions to be determined at a given instant at the push of a button. This included determination of tunnel pressures, speed, density, temperature, and viscosity. Other aspects of the experimental equipment included the set up of a CCD video camera with a video frame grabber, monitor, and VCR to capture the motion. A strobe light was used to highlight the smoke that was used to visualize the flow. Additional effort was put into creating a scale drawing of another tunnel on site and a limited literature search in the area of active flow control.

  10. Apparatus for controlling fluid flow in a conduit wall

    DOEpatents

    Glass, S. Jill; Nicolaysen, Scott D.; Beauchamp, Edwin K.

    2003-05-13

    A frangible rupture disk and mounting apparatus for use in blocking fluid flow, generally in a fluid conducting conduit such as a well casing, a well tubing string or other conduits within subterranean boreholes. The disk can also be utilized in above-surface pipes or tanks where temporary and controllable fluid blockage is required. The frangible rupture disk is made from a pre-stressed glass with controllable rupture properties wherein the strength distribution has a standard deviation less than approximately 5% from the mean strength. The frangible rupture disk has controllable operating pressures and rupture pressures.

  11. Application of stability theory to laminar flow control

    NASA Technical Reports Server (NTRS)

    Hefner, J. N.; Bushnell, D. M.

    1979-01-01

    The paper summarizes the state-of-the-art for application of stability theory to laminar flow control using suction, wall temperature and/or favorable pressure gradient ('natural laminar flow'). Discussions include current LFC problem areas requiring stability analyses, methods of relating stability theory to transition with results from data and theory comparisons available thus far, and a summary of low disturbance data available for theory calibration on swept wings. Critical issues highlighted are problems peculiar to suction LFC on high performance transonic wings and application of the e-to-the-n-power method to both low and high speed flight data.

  12. Control of flow separation in airfoil/wing design applications

    NASA Technical Reports Server (NTRS)

    Gally, Thomas A.

    1994-01-01

    Existing aerodynamic design methods have generally concentrated on the optimization of airfoil or wing shapes to produce a minimum drag while satisfying some basic constraints such as lift, pitching moment, or thickness. Since the minimization of drag almost always precludes the existence of separated flow, the evaluation and validation of these design methods for their robustness and accuracy when separated flow is present has not been aggressively pursued. However, two new applications for these design tools may be expected to include separated flow and the issues of aerodynamic design with this feature must be addressed. The first application of the aerodynamic design tools is the design of airfoils or wings to provide an optimal performance over a wide range of flight conditions (multipoint design). While the definition of 'optimal performance' in the multipoint setting is currently being hashed out, it is recognized that given a wide enough range of flight conditions, it will not be possible to ensure a minimum drag constraint at all conditions, and in fact some amount of separated flow (presumably small) may have to be allowed at the more demanding flight conditions. Thus a multipoint design method must be tolerant of the existence of separated flow and may include some controls upon its extent. The second application is in the design of wings with extended high speed buffet boundaries of their flight envelopes. Buffet occurs on a wing when regions of flow separation have grown to the extent that their time varying pressures induce possible destructive effects upon the wing structure or adversely effect either the aircraft controllability or the passenger comfort. A conservative approach to the expansion of the buffet flight boundary is to simply expand the flight envelope of nonseparated flow under the assumption that buffet will also thus be alleviated. However, having the ability to design a wing with separated flow and thus to control the location, extent

  13. Effect of whey protein on blood lipid profiles: a meta-analysis of randomized controlled trials.

    PubMed

    Zhang, J-W; Tong, X; Wan, Z; Wang, Y; Qin, L-Q; Szeto, I M Y

    2016-08-01

    Previous studies have suggested that whey supplementation may have beneficial effects on lipid profiles, although results were inconsistent. A literature search was performed in March 2015 for randomized controlled trials observing the effects of whey protein and its derivatives on circulating levels of triacylglycerol (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). A meta-analysis was subsequently conducted. The meta-analysis results of 13 trials showed that whey supplementation significantly reduced the circulating TG level by 0.11 mmol/l (95% CI: -0.21, 0 mmol/l), whereas the whey protein had no effects on circulating TC (-0.11 mmol/l, 95% CI: -0.27, 0.05 mmol/l), LDL-C (-0.08 mmol/l, 95% CI: -0.23, 0.07 mmol/l) and HDL-C (0.01 mmol/l, 95% CI: -0.04, 0.05 mmol/l). Subgroup analysis showed that significant TG reduction disappeared in participants with low body mass index, low supplemental whey dose or under exercise training/energy restriction during the trial. No evidence of heterogeneity across studies and publication bias was observed. In conclusion, our findings demonstrated that the effects of whey protein supplementation were modest, with an overall lowering effect on TG but no effect on TC, LDL-C and HDL-C. PMID:27026427

  14. TCP flow control using link layer information in mobile networks

    NASA Astrophysics Data System (ADS)

    Koga, Hiroyuki; Kawahara, Kenji; Oie, Yuji

    2002-07-01

    Mobile Networks have been expanding and IMT-2000 further increases their available bandwidth over wireless links. However, TCP, which is a reliable end-to-end transport protocol, is tuned to perform well in wired networks where bit error rates are very low and packet loss occurs mostly because of congestion. Although a TCP sender can execute flow control to utilize as much available bandwidth as possible in wired networks, it cannot work well in wireless networks characterized by high bit error rates. In the next generation mobile systems, sophisticated error recovery technologies of FEC and ARQ are indeed employed over wireless links, i.e., over Layer 2, to avoid performance degradation of upper layers. However, multiple retransmissions by Layer 2 ARQ can adversely increase transmission delay of TCP segments, which will further make TCP unnecessarily increase RTO (Retransmission TimeOut). Furthermore, a link bandwidth assigned to TCP flows can change in response to changing air conditions to use wireless links efficiently. TCP thus has to adapt its transmission rate according to the changing available bandwidth. The major goal of this study is to develop a receiver-based effective TCP flow control without any modification on TCP senders, which are probably connected with wired networks. For this end, we propose a TCP flow control employing some Layer 2 information on a wireless link at the mobile station. Our performance evaluation of the proposed TCP shows that the receiver-based TCP flow control can moderate the performance degradation very well even if FER on Layer 2 is high.

  15. Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway

    PubMed Central

    Taketa, Shin; Amano, Satoko; Tsujino, Yasuhiro; Sato, Tomohiko; Saisho, Daisuke; Kakeda, Katsuyuki; Nomura, Mika; Suzuki, Toshisada; Matsumoto, Takashi; Sato, Kazuhiro; Kanamori, Hiroyuki; Kawasaki, Shinji; Takeda, Kazuyoshi

    2008-01-01

    In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (i) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion. PMID:18316719

  16. Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

    PubMed Central

    Li, Ruixi; Sun, Ruobai; Hicks, Glenn R.; Raikhel, Natasha V.

    2015-01-01

    The vacuole is the most prominent compartment in plant cells and is important for ion and protein storage. In our effort to search for key regulators in the plant vacuole sorting pathway, ribosomal large subunit 4 (rpl4d) was identified as a translational mutant defective in both vacuole trafficking and normal development. Polysome profiling of the rpl4d mutant showed reduction in polysome-bound mRNA compared with wild-type, but no significant change in the general mRNA distribution pattern. Ribsomal profiling data indicated that genes in the lipid metabolism pathways were translationally down-regulated in the rpl4d mutant. Live imaging studies by Nile red staining suggested that both polar and nonpolar lipid accumulation was reduced in meristem tissues of rpl4d mutants. Pharmacological evidence showed that sterol and sphingolipid biosynthetic inhibitors can phenocopy the defects of the rpl4d mutant, including an altered vacuole trafficking pattern. Genetic evidence from lipid biosynthetic mutants indicates that alteration in the metabolism of either sterol or sphingolipid biosynthesis resulted in vacuole trafficking defects, similar to the rpl4d mutant. Tissue-specific complementation with key enzymes from lipid biosynthesis pathways can partially rescue both vacuole trafficking and auxin-related developmental defects in the rpl4d mutant. These results indicate that lipid metabolism modulates auxin-mediated tissue differentiation and endomembrane trafficking pathways downstream of ribosomal protein function. PMID:25535344

  17. Bidirectional control of sphingomyelinase activity and surface topography in lipid monolayers.

    PubMed

    Fanani, María Laura; Härtel, Steffen; Oliveira, Rafael G; Maggio, Bruno

    2002-12-01

    Lipid lateral organization is increasingly found to modulate membrane-bound enzymes. We followed in real time the reaction course of sphingomyelin (SM) degradation by Bacillus cereus sphingomyelinase (SMase) of lipid monolayers by epifluorescence microscopy. There is evidence that formation of ceramide (Cer), a lipid second messenger, drives structural reorganization of membrane lipids. Our results provide visual evidence that SMase activity initially alters surface topography by inducing phase separation into condensed (Cer-enriched) and expanded (SM-enriched) domains. The Cer-enriched phase grows steadily as the reaction proceeds at a constant rate. The surface topography derived from the SMase-driven reaction was compared with, and found to differ from, that of premixed SM/Cer monolayers of the same lipid composition, indicating that substantial information content is stored depending on the manner in which the surface was generated. The long-range topographic changes feed back on the kinetics of Smase, and the onset of condensed-phase percolation is temporally correlated with a rapid drop of reaction rate. These observations reveal a bidirectional influence and communication between effects taking place at the local molecular level and the supramolecular organization. The results suggest a novel biocatalytic-topographic mechanism in which a surface enzymatic activity can influence the function of amphitropic proteins important for cell function. PMID:12496108

  18. Compartmentalization of the exocyst complex in lipid rafts controls Glut4 vesicle tethering.

    PubMed

    Inoue, Mayumi; Chiang, Shian-Huey; Chang, Louise; Chen, Xiao-Wei; Saltiel, Alan R

    2006-05-01

    Lipid raft microdomains act as organizing centers for signal transduction. We report here that the exocyst complex, consisting of Exo70, Sec6, and Sec8, regulates the compartmentalization of Glut4-containing vesicles at lipid raft domains in adipocytes. Exo70 is recruited by the G protein TC10 after activation by insulin and brings with it Sec6 and Sec8. Knockdowns of these proteins block insulin-stimulated glucose uptake. Moreover, their targeting to lipid rafts is required for glucose uptake and Glut4 docking at the plasma membrane. The assembly of this complex also requires the PDZ domain protein SAP97, a member of the MAGUKs family, which binds to Sec8 upon its translocation to the lipid raft. Exocyst assembly at lipid rafts sets up targeting sites for Glut4 vesicles, which transiently associate with these microdomains upon stimulation of cells with insulin. These results suggest that the TC10/exocyst complex/SAP97 axis plays an important role in the tethering of Glut4 vesicles to the plasma membrane in adipocytes. PMID:16525015

  19. Bidirectional control of sphingomyelinase activity and surface topography in lipid monolayers.

    PubMed Central

    Fanani, María Laura; Härtel, Steffen; Oliveira, Rafael G; Maggio, Bruno

    2002-01-01

    Lipid lateral organization is increasingly found to modulate membrane-bound enzymes. We followed in real time the reaction course of sphingomyelin (SM) degradation by Bacillus cereus sphingomyelinase (SMase) of lipid monolayers by epifluorescence microscopy. There is evidence that formation of ceramide (Cer), a lipid second messenger, drives structural reorganization of membrane lipids. Our results provide visual evidence that SMase activity initially alters surface topography by inducing phase separation into condensed (Cer-enriched) and expanded (SM-enriched) domains. The Cer-enriched phase grows steadily as the reaction proceeds at a constant rate. The surface topography derived from the SMase-driven reaction was compared with, and found to differ from, that of premixed SM/Cer monolayers of the same lipid composition, indicating that substantial information content is stored depending on the manner in which the surface was generated. The long-range topographic changes feed back on the kinetics of Smase, and the onset of condensed-phase percolation is temporally correlated with a rapid drop of reaction rate. These observations reveal a bidirectional influence and communication between effects taking place at the local molecular level and the supramolecular organization. The results suggest a novel biocatalytic-topographic mechanism in which a surface enzymatic activity can influence the function of amphitropic proteins important for cell function. PMID:12496108

  20. Enabling Technologies for Microfluidic Flow Control and Detection

    NASA Astrophysics Data System (ADS)

    Leslie, Daniel Christopher

    Advances in microfluidic technologies have expanded conventional chemical and biological techniques to the point where we can envision rapid, inexpensive and portable analysis. Among the numerous challenges in the development of portable, chip-based technologies are simple flow control and detection strategies, which will be essential to widespread acceptance and implementation at both the point-of-care and in locales with limited facilities/resources. The research presented in this dissertation is focused on the development of precise flow control techniques and new, simplified detection technologies aimed at addressing these challenges. An introduction to the concepts important to microfluidics and a brief history to the field are presented in Chapter 1. Chapter 2 will present the development of a technique for the precise control of small volumes of liquids, where well-studied electrical circuit concepts are employed to create frequency-dependent microfluidic circuits. In this system, elastomeric thin films act as fluidic capacitors and diodes, which, when combined with resistors (channels), make fluidic circuits that are described by analytical models. Metering of two separate chemical inputs with a single oscillatory pneumatic control line is demonstrated by combining simple fluidic circuits (i.e., band-pass filters) to significantly reduce the external hardware required for microfluidic flow control. In order to quantify multiple flow profiles in microfluidic circuits, a novel multiplexed flow measurement method using visible dyes is introduced in Chapter 3 and rapidly determines individual flow in connected channels, post-fabrication device quality and solution viscosity. Another thrust of this dissertation research has been to develop miniaturized bioanalytical systems. Chapter 4 describes the adaption of a nucleic-acid-tagged antibody protein detection reaction to a microfluidic platform for detection of down to 5 E. coli O157:H7 cells. Furthermore, a

  1. Modeling and control of flow-induced vibrations of a flexible hydrofoil in viscous flow

    NASA Astrophysics Data System (ADS)

    Caverly, Ryan James; Li, Chenyang; Chae, Eun Jung; Forbes, James Richard; Young, Yin Lu

    2016-06-01

    In this paper, a reduced-order model (ROM) of the flow-induced vibrations of a flexible cantilevered hydrofoil is developed and used to design an active feedback controller. The ROM is developed using data from high-fidelity viscous fluid-structure interaction (FSI) simulations and includes nonlinear terms to accurately capture the effect of lock-in. An active linear quadratic Gaussian (LQG) controller is designed based on a linearization of the ROM and is implemented in simulation with the ROM and the high-fidelity viscous FSI model. A controller saturation method is also presented that ensures that the control force applied to the system remains within a prescribed range. Simulation results demonstrate that the LQG controller successfully suppresses vibrations in both the ROM and viscous FSI simulations using a reasonable amount of control force.

  2. Optimization of the C11-BODIPY(581/591) dye for the determination of lipid oxidation in Chlamydomonas reinhardtii by flow cytometry.

    PubMed

    Cheloni, Giulia; Slaveykova, Vera I

    2013-10-01

    Lipid oxidation is a recognized end point for the study of oxidative stress and is an important parameter to describe the mode of micropollutant action on aquatic microorganisms. Therefore, the development of quick and reliable methodologies probing the oxidative stress and damage in living cells is highly sought. In the present proof-of-concept work, we examined the potential of the fluorescent dye C11-BODIPY(591/581) to probe lipid oxidation in the green microalga Chlamydomonas reinhardtii. C11-BODIPY(591/581) staining was combined with flow cytometry measurements to obtain multiparameter information on cellular features and oxidative stress damage within single cells. First, staining conditions were optimized by exploring the capability of the dye to stain algal cells under increasing cell and dye concentrations and different staining procedures. Then lipid oxidation in algae induced by short- and long-term exposures to the three metallic micropollutants, copper, mercury, and nanoparticulate copper oxide, and the two organic contaminants, diethyldithiocarbamate (DDC) and diuron was determined. In this work we pointed out C11-BODIPY(591/581) applicability in a wide range of exposure conditions, including studies of oxidation as a function of time and that it is suitable for in vivo measurements of lipid oxidation due to its high permeation and stability in cells and its low interference with algal autofluorescence. © 2013 International Society for Advancement of Cytometry. PMID:23943236

  3. Vacuum rated flow controllers for inert gas ion engines

    NASA Technical Reports Server (NTRS)

    Pless, L. C.

    1987-01-01

    Electrical propulsion systems which use a gas as a propellant require a gas flowmeter/controller which is capable of operating in a vacuum environment. The presently available instruments in the required flow ranges are designed and calibrated for use at ambient pressure. These instruments operate by heating a small diameter tube through which the gas is flowing and then sensing the change in temperature along the length of the tube. This temperature change is a function of the flow rate and the gas heat capacity. When installed in a vacuum, the change in the external thermal characteristics cause the tube to overheat and the temperature sensors are then operating outside their calibrated range. In addition, the variation in heat capacity with temperature limit the accuracy obtainable. These problems and the work in progress to solve them are discussed.

  4. Numerical Studies of a Fluidic Diverter for Flow Control

    NASA Technical Reports Server (NTRS)

    Gokoglu, Suleyman A.; Kuczmarski, Maria A.; Culley, Dennis E.; Raghu, Surya

    2009-01-01

    The internal flow structure in a specific fluidic diverter is studied over a range from low subsonic to sonic inlet conditions by a time-dependent numerical analysis. The understanding will aid in the development of fluidic diverters with minimum pressure losses and advanced designs of flow control actuators. The velocity, temperature and pressure fields are calculated for subsonic conditions and the self-induced oscillatory behavior of the flow is successfully predicted. The results of our numerical studies have excellent agreement with our experimental measurements of oscillation frequencies. The acoustic speed in the gaseous medium is determined to be a key factor for up to sonic conditions in governing the mechanism of initiating the oscillations as well as determining its frequency. The feasibility of employing plasma actuation with a minimal perturbation level is demonstrated in steady-state calculations to also produce oscillation frequencies of our own choosing instead of being dependent on the fixed-geometry fluidic device.

  5. Transonic flow control by means of local energy deposition

    NASA Astrophysics Data System (ADS)

    Aul'Chenko, S. M.; Zamuraev, V. P.; Kalinina, A. P.

    2011-11-01

    Experimental data for the feasibility of transonic flow control by means of energy deposition are generalized. Energy supplied to the immediate vicinity of a body in stream before a compression shock is found to result in the nonlinear interaction of introduced disturbances with the shock and the surface in zones extended along the surface. A new, explosive gasdynamic mechanism behind the shift of the compression shock is discovered. It is shown that the nonlinear character of the interaction may considerably decrease the wave resistance of, e.g., transonic airfoils. It is found that energy supply from without stabilizes a transonic flow about an airfoil—the effect similar to the Khristianovich stabilization effect. The dependence of the energy deposition optimal frequency on the energy source parameters and Mach number of the incoming flow at which the resistance drops to the greatest extent is obtained. The influence of the real thermodynamic properties and viscosity of air is studied.

  6. Magnetic Amplifier-Based Power-Flow Controller

    SciTech Connect

    Dimitrovski, Aleksandar; Li, Zhi; Ozpineci, Burak

    2015-02-05

    The concept of the magnetic amplifier, a common electromagnetic device in electronic applications in the past, has seldom been used in power systems. In this paper, we introduce the magnetic amplifier-based power-flow controller (MAPFC), an innovative low-cost device that adopts the idea of the magnetic amplifier for power-flow control applications. The uniqueness of MAPFC is in the use of the magnetization of the ferromagnetic core, shared by an ac and a dc winding, as the medium to control the ac winding reactance inserted in series with the transmission line to be controlled. Large power flow in the line can be regulated by the small dc input to the dc winding. Moreover, a project on the R&D of an MAPFC has been funded by the U.S. Department of Energy (DOE) and conducted by the Oak Ridge National Laboratory (ORNL), the University of Tennessee-Knoxville, and Waukesha Electric Systems, Inc. since early 2012. Findings from the project are presented along with some results obtained in a laboratory environment.

  7. Magnetic Amplifier-Based Power-Flow Controller

    DOE PAGESBeta

    Dimitrovski, Aleksandar; Li, Zhi; Ozpineci, Burak

    2015-02-05

    The concept of the magnetic amplifier, a common electromagnetic device in electronic applications in the past, has seldom been used in power systems. In this paper, we introduce the magnetic amplifier-based power-flow controller (MAPFC), an innovative low-cost device that adopts the idea of the magnetic amplifier for power-flow control applications. The uniqueness of MAPFC is in the use of the magnetization of the ferromagnetic core, shared by an ac and a dc winding, as the medium to control the ac winding reactance inserted in series with the transmission line to be controlled. Large power flow in the line can bemore » regulated by the small dc input to the dc winding. Moreover, a project on the R&D of an MAPFC has been funded by the U.S. Department of Energy (DOE) and conducted by the Oak Ridge National Laboratory (ORNL), the University of Tennessee-Knoxville, and Waukesha Electric Systems, Inc. since early 2012. Findings from the project are presented along with some results obtained in a laboratory environment.« less

  8. Adaptive Identification and Control of Flow-Induced Cavity Oscillations

    NASA Technical Reports Server (NTRS)

    Kegerise, M. A.; Cattafesta, L. N.; Ha, C.

    2002-01-01

    Progress towards an adaptive self-tuning regulator (STR) for the cavity tone problem is discussed in this paper. Adaptive system identification algorithms were applied to an experimental cavity-flow tested as a prerequisite to control. In addition, a simple digital controller and a piezoelectric bimorph actuator were used to demonstrate multiple tone suppression. The control tests at Mach numbers of 0.275, 0.40, and 0.60 indicated approx. = 7dB tone reductions at multiple frequencies. Several different adaptive system identification algorithms were applied at a single freestream Mach number of 0.275. Adaptive finite-impulse response (FIR) filters of orders up to N = 100 were found to be unsuitable for modeling the cavity flow dynamics. Adaptive infinite-impulse response (IIR) filters of comparable order better captured the system dynamics. Two recursive algorithms, the least-mean square (LMS) and the recursive-least square (RLS), were utilized to update the adaptive filter coefficients. Given the sample-time requirements imposed by the cavity flow dynamics, the computational simplicity of the least mean squares (LMS) algorithm is advantageous for real-time control.

  9. [Factors affecting the control of blood pressure and lipid levels in patients with cardiovascular disease: the PREseAP Study].

    PubMed

    Orozco-Beltrán, Domingo; Brotons, Carlos; Moral, Irene; Soriano, Nuria; Del Valle, María A; Rodríguez, Ana I; Pepió, Josep M; Pastor, Ana

    2008-03-01

    The aim of this observational study was to identify factors influencing the control of blood pressure (i.e., <140/90 mmHg, or <130/80 mmHg in diabetic patients) and low-density lipoprotein (LDL) cholesterol level (<100 mg/dL) in 1223 patients with cardiovascular disease. Overall, 70.2% of patients were men, and their mean age was 66.4 years. Blood pressure was poorly controlled in 50.9% (95% confidence interval [CI], 46.9%-54.8%) and the LDL cholesterol level was poorly controlled in 60.1% (95% CI, 56.3%-63.9%). Determinants of poor blood pressure control were diabetes, hypertension, no previous diagnosis of heart failure, previous diagnosis of peripheral artery disease or stroke, obesity, and no lipid-lowering treatment. Determinants of poor LDL cholesterol control were no lipid-lowering treatment, no previous diagnosis of ischemic heart disease, no antihypertensive treatment, and dyslipidemia. The factors affecting blood pressure control were different from those affecting LDL cholesterol control, an observation that should be taken into account when implementing treatment recommendations for achieving therapeutic objectives in secondary prevention. PMID:18361907

  10. Control mechanisms operating for lipid biosynthesis differ in oil-palm (Elaeis guineensis Jacq.) and olive (Olea europaea L.) callus cultures.

    PubMed Central

    Ramli, Umi S; Baker, Darren S; Quant, Patti A; Harwood, John L

    2002-01-01

    As a prelude to detailed flux control analysis of lipid synthesis in plants, we have examined the latter in tissue cultures from two important oil crops, olive (Olea europaea L.) and oil palm (Elaeis guineensis Jacq.). Temperature was used to manipulate the overall rate of lipid formation in order to characterize and validate the system to be used for analysis. With [1-14C]acetate as a precursor, an increase in temperature from 20 to 30 degrees C produced nearly a doubling of total lipid labelling. This increase in total lipids did not change the radioactivity in the intermediate acyl-(acyl carrier protein) or acyl-CoA pools, indicating that metabolism of these pools did not exert any significant constraint for overall synthesis. In contrast, there were some differences in the proportional labelling of fatty acids and of lipid classes at the two temperatures. The higher temperature caused a decrease in polyunsaturated fatty acid labelling and an increase in the proportion of triacylglycerol labelling in both calli. The intermediate diacylglycerol was increased in olive, but not in oil palm. Overall the data indicate the suitability of olive and oil-palm cultures for the study of lipid synthesis and indicate that de novo fatty acid synthesis may exert more flux control than complex lipid assembly. In olive, diacylglycerol acyltransferase may exert significant flux control when lipid synthesis is rapid. PMID:12023881

  11. Model reduction and feedback control of transitional channel flow

    NASA Astrophysics Data System (ADS)

    Ilak, Milos

    This dissertation examines the use of reduced-order models for design of linear feedback controllers for fluid flows. The focus is on transitional channel flow, a canonical shear flow case with a simple geometry yet complex dynamics. Reduced-order models of the linearized Navier-Stokes equations, which describe the evolution of perturbations in transitional channel flow, are computed using two methods for snapshot-based balanced truncation, Balanced Proper Orthogonal Decomposition (BPOD) and Eigensystem Realization Algorithm (ERA). The performance of these models in feedback control is evaluated in both linearized and nonlinear Direct Numerical Simulations (DNS) of channel flow. The first part of the dissertation describes the application of BPOD to very large systems, and the detailed evaluation of the resulting reduced-order models. Exact balanced truncation, a standard method from control theory, is not computationally tractable for very large systems, such as those typically encountered in fluid flow simulations. The BPOD method, introduced by Rowley (2005), provides a close approximation. We first show that the approximation is indeed close by applying the method to a 1-D linear perturbation to channel flow at a single spatial wavenumber pair, for which exact balanced truncation is tractable. Next, as the first application of BPOD to a very high-dimensional linear system, we show that reduced-order BPOD models of a localized 3-D perturbation capture the dynamics very well. Moreover, the BPOD models significantly outperform standard Proper Orthogonal Decomposition (POD) models, as illustrated by a striking example where models using the POD modes that capture most of the perturbation energy fail to capture the perturbation dynamics. Next, reduced-order models of a complete control system for linearized channel flow are obtained using ERA, a computationally efficient method that results in the same reduced-order models as BPOD. Linear Quadratic Gaussian (LQG

  12. Lipid coated upconverting nanoparticles as NIR remote controlled transducer for simultaneous photodynamic therapy and cell imaging.

    PubMed

    Wang, Hanjie; Dong, Chunhong; Zhao, Peiqi; Wang, Sheng; Liu, Zhongyun; Chang, Jin

    2014-05-15

    The application of photodynamic therapy in deep tissue is constrained by some pending problems, such as the limited penetration depth of excitation light and lacking of targeting ability. In this paper, a new kind of lipid coated upconverting nanoparticles consisiting of upconerting nanocrystal core and targeted lipid polymer shell was first reported for NIR triggered photodynamic therapy and cell imaging simultaneously. The lipid coated upconverting nanoparticles offers advantages to overcome the problem mentioned above. The UCN core works as a transducer to convert deeply penetrating near-infrared light to visible lights for activating photosensitizer and cell fluorescence imaging simultaneously. The amphiphilic lipid polymer RGD peptide conjugated poly (maleic anhydride-alt-1-octadecene) grafted dioleoyl l-α-phosphatidylethanolamine (RGD-PMAO-DOPE) acts as a shield. It can protect the system from catching by RES and target the whole system to the lesions. The experiment results show that the lipid coated upconverting nanoparticle is individual nanosphere with an average size of 20 nm. The drug loading can reach 9%. After NIR exposed, the MC540 was activated to produce singlet oxygen (ROS) successfully by the upconverting fluorescence emitted from UCN. Importantly, compared with nanoparticle without RGD decoration, the lipid coated upconverting nanoparticle can co-deliver the MC540 and UCNs into the same cell with higher efficiency. Besides, the MC540 loaded UCN/RGD-PMAO-DOPE nanoparticles showed significant inhibitory effect on tumor cells after NIR shining. Our data suggests that MC540 loaded UCN/RGD-PMAO-DOPE nanoparticle may be a useful nanoplatform for future PDT treatment in deep-cancer therapy. PMID:24657139

  13. Factors Controlling the Stable Nitrogen Isotopic Composition (δ15N) of Lipids in Marine Animals.

    PubMed

    Svensson, Elisabeth; Schouten, Stefan; Hopmans, Ellen C; Middelburg, Jack J; Sinninghe Damsté, Jaap S

    2016-01-01

    Lipid extraction of biomass prior to stable isotope analysis is known to cause variable changes in the stable nitrogen isotopic composition (δ15N) of residual biomass. However, the underlying factors causing these changes are not yet clear. Here we address this issue by comparing the δ15N of bulk and residual biomass of several marine animal tissues (fish, crab, cockle, oyster, and polychaete), as well as the δ15N of the extracted lipids. As observed previously, lipid extraction led to a variable offset in δ15N of biomass (differences ranging from -2.3 to +1.8 ‰). Importantly, the total lipid extract (TLE) was highly depleted in 15N compared to bulk biomass, and also highly variable (differences ranging from -14 to +0.7 ‰). The TLE consisted mainly of phosphatidylcholines, a group of lipids with one nitrogen atom in the headgroup. To elucidate the cause for the 15N-depletion in the TLE, the δ15N of amino acids was determined, including serine because it is one of the main sources of nitrogen to N-containing lipids. Serine δ15N values differed by -7 to +2 ‰ from bulk biomass δ15N, and correlated well with the 15N depletion in TLEs. On average, serine was less depleted (-3‰) than the TLE (-7 ‰), possibly due to fractionation during biosynthesis of N-containing headgroups, or that other nitrogen-containing compounds, such as urea and choline, or recycled nitrogen contribute to the nitrogen isotopic composition of the TLE. The depletion in 15N of the TLE relative to biomass increased with the trophic level of the organisms. PMID:26731720

  14. Factors Controlling the Stable Nitrogen Isotopic Composition (δ15N) of Lipids in Marine Animals

    PubMed Central

    Svensson, Elisabeth; Schouten, Stefan; Hopmans, Ellen C.; Middelburg, Jack J.; Sinninghe Damsté, Jaap S.

    2016-01-01

    Lipid extraction of biomass prior to stable isotope analysis is known to cause variable changes in the stable nitrogen isotopic composition (δ15N) of residual biomass. However, the underlying factors causing these changes are not yet clear. Here we address this issue by comparing the δ15N of bulk and residual biomass of several marine animal tissues (fish, crab, cockle, oyster, and polychaete), as well as the δ15N of the extracted lipids. As observed previously, lipid extraction led to a variable offset in δ15N of biomass (differences ranging from -2.3 to +1.8 ‰). Importantly, the total lipid extract (TLE) was highly depleted in 15N compared to bulk biomass, and also highly variable (differences ranging from -14 to +0.7 ‰). The TLE consisted mainly of phosphatidylcholines, a group of lipids with one nitrogen atom in the headgroup. To elucidate the cause for the 15N-depletion in the TLE, the δ15N of amino acids was determined, including serine because it is one of the main sources of nitrogen to N-containing lipids. Serine δ15N values differed by -7 to +2 ‰ from bulk biomass δ15N, and correlated well with the 15N depletion in TLEs. On average, serine was less depleted (-3‰) than the TLE (-7 ‰), possibly due to fractionation during biosynthesis of N-containing headgroups, or that other nitrogen-containing compounds, such as urea and choline, or recycled nitrogen contribute to the nitrogen isotopic composition of the TLE. The depletion in 15N of the TLE relative to biomass increased with the trophic level of the organisms. PMID:26731720

  15. Optimal Control of Airfoil Flow Separation using Fluidic Excitation

    NASA Astrophysics Data System (ADS)

    Shahrabi, Arireza F.

    This thesis deals with the control of flow separation around a symmetric airfoils with the aid of multiple synthetic jet actuators (SJAs). CFD simulation methods have been implemented to uncover the flow separation regimes and associated properties such as frequencies and momentum ratio. In the first part of the study, the SJA was studied thoroughly. Large Eddy Simulations (LES) were performed for one individual cavity; the time history of SJA of the outlet velocity profile and the net momentum imparted to the flow were analyzed. The studied SJA is asymmetrical and operates with the aid of a piezoelectric (PZT) ceramic circular plate actuator. A three-dimensional mesh for the computational domain of the SJA and the surrounding volume was developed and was used to evaluate the details of the airflow conditions inside the SJA as well as at the outlet. The vibration of the PZT ceramic actuator was used as a boundary condition in the computational model to drive the SJA. Particular attention was given to developing a predictive model of the SJA outlet velocity. Results showed that the SJA velocity output is correlated to the PZT ceramic plate vibration, especially for the first frequency mode. SJAs are a particular class of zero net mass flux (ZNMF) fluidic devices with net imparted momentum to the flow. The net momentum imparted to the flow in the separated region is such that positive enhancement during AFC operations is achieved. Flows around the NACA 0015 airfoil were simulated for a range of operating conditions. Attention was given to the active open and closed loop control solutions for an airfoil with SJA at different angles of attack and flap angles. A large number of simulations using RANS & LES models were performed to study the effects of the momentum ratio (Cμ) in the range of 0 to 11% and of the non-dimensional frequency, F+, in the range of 0 to 2 for the control of flow separation at a practical angle of attack and flap angle. The optimum value of C

  16. Control of cutaneous blood flow by central nervous system.

    PubMed

    Ootsuka, Youichirou; Tanaka, Mutsumi

    2015-01-01

    Hairless skin acts as a heat exchanger between body and environment, and thus greatly contributes to body temperature regulation by changing blood flow to the skin (cutaneous) vascular bed during physiological responses such as cold- or warm-defense and fever. Cutaneous blood flow is also affected by alerting state; we 'go pale with fright'. The rabbit ear pinna and the rat tail have hairless skin, and thus provide animal models for investigating central pathway regulating blood flow to cutaneous vascular beds. Cutaneous blood flow is controlled by the centrally regulated sympathetic nervous system. Sympathetic premotor neurons in the medullary raphé in the lower brain stem are labeled at early stage after injection of trans-synaptic viral tracer into skin wall of the rat tail. Inactivation of these neurons abolishes cutaneous vasomotor changes evoked as part of thermoregulatory, febrile or psychological responses, indicating that the medullary raphé is a common final pathway to cutaneous sympathetic outflow, receiving neural inputs from upstream nuclei such as the preoptic area, hypothalamic nuclei and the midbrain. Summarizing evidences from rats and rabbits studies in the last 2 decades, we will review our current understanding of the central pathways mediating cutaneous vasomotor control. PMID:27227053

  17. Controlling Compressor Vane Flow Vectoring Angles at Transonic Speeds

    NASA Astrophysics Data System (ADS)

    Munson, Matthew; Rempfer, Dietmar; Williams, David; Acharya, Mukund

    2003-11-01

    The ability to control flow separation angles from compressor inlet guide vanes with a Coanda-type actuator is demonstrated using both wind tunnel experiments and finite element simulations. Vectoring angles up to 40 degrees from the uncontrolled baseline state were measured with helium schlieren visualization at transonic Mach numbers ranging from 0.1 to 0.6, and with airfoil chord Reynolds numbers ranging from 89,000 to 710,000. The magnitude of the vectoring angle is shown to depend upon the geometry of the trailing edge, and actuator slot size, and the momentum flux coefficient. Under certain conditions the blowing has no effect on the vectoring angle indicating that the Coanda effect is not present. DNS simulations with the finite element method investigated the effects of geometry changes and external flow. Continuous control of the vectoring angle is demonstrated, which has important implications for application to rotating machinery. The technique is shown to reduce the stall flow coefficient by 15 percent in an axial flow compressor.

  18. Control of cutaneous blood flow by central nervous system

    PubMed Central

    Ootsuka, Youichirou; Tanaka, Mutsumi

    2015-01-01

    Hairless skin acts as a heat exchanger between body and environment, and thus greatly contributes to body temperature regulation by changing blood flow to the skin (cutaneous) vascular bed during physiological responses such as cold- or warm-defense and fever. Cutaneous blood flow is also affected by alerting state; we ‘go pale with fright’. The rabbit ear pinna and the rat tail have hairless skin, and thus provide animal models for investigating central pathway regulating blood flow to cutaneous vascular beds. Cutaneous blood flow is controlled by the centrally regulated sympathetic nervous system. Sympathetic premotor neurons in the medullary raphé in the lower brain stem are labeled at early stage after injection of trans-synaptic viral tracer into skin wall of the rat tail. Inactivation of these neurons abolishes cutaneous vasomotor changes evoked as part of thermoregulatory, febrile or psychological responses, indicating that the medullary raphé is a common final pathway to cutaneous sympathetic outflow, receiving neural inputs from upstream nuclei such as the preoptic area, hypothalamic nuclei and the midbrain. Summarizing evidences from rats and rabbits studies in the last 2 decades, we will review our current understanding of the central pathways mediating cutaneous vasomotor control. PMID:27227053

  19. Control of continuous polyhydroxybutyrate synthesis using calorimetry and flow cytometry.

    PubMed

    Maskow, Thomas; Müller, Susann; Lösche, Andreas; Harms, Hauke; Kemp, Richard

    2006-02-20

    The substrate-carbon flow can be controlled in continuous bioreactor cultures by the medium composition, for example, by the C/N ratio. The carbon distribution is optimal when a maximum fraction flows into the desired product and the residual is just sufficient to compensate for the dilution of the microbial catalyst. Undershooting of the latter condition is reflected immediately by changes in the Gibbs energy dissipation and cellular states. Two calorimetric measurement principles were applied to optimize the continuous synthesis of polyhydroxybutyrate (PHB) by Variovorax paradoxus DSM4065 during growth with constantly increasing supply rates of fructose or toxic phenol. Firstly, the changed slope of the heat production rate in a complete heat balanced bioreactor (CHB) indicated optimum carbon channeling into PHB. The extent of the alteration depended directly on the toxic properties of the substrate. Secondly, a flow through calorimeter was connected with the bioreactor as a "measurement loop." The optimum substrate carbon distribution was indicated by a sudden change in the heat production rate independent of substrate toxicity. The sudden change was explained mathematically and exploited for the long-term control of phenol conversion into PHB. LASER flow cytometry measurements distinguished between subpopulations with completely different PHB-content. Populations grown on fructose preserved a constant ratio of two subpopulations with double and quadruple sets of DNA. Cells grown on phenol comprised a third subpopulation with a single DNA set. Rising phenol concentrations caused this subpopulation to increase. It may thus be considered as an indicator of chemostress. PMID:16245347

  20. Integration and Modulation of Intercellular Signaling Underlying Blood Flow Control

    PubMed Central

    Segal, Steven S.

    2015-01-01

    Vascular resistance networks control tissue blood flow in concert with regulating arterial perfusion pressure. In response to increased metabolic demand, vasodilation arising in arteriolar networks ascends to encompass proximal feed arteries. By reducing resistance upstream, ascending vasodilation (AVD) increases blood flow into the microcirculation. Once initiated [e.g., through local activation of K+ channels in endothelial cells (ECs)], hyperpolarization is conducted through gap junctions along the endothelium. Via EC projections through the internal elastic lamina, hyperpolarization spreads into the surrounding smooth muscle cells (SMCs) through myoendothelial gap junctions (MEGJs) to promote their relaxation. Intercellular signaling through electrical signal transmission (i.e., cell-to-cell conduction) can thereby coordinate vasodilation along and among the branches of microvascular resistance networks. Perivascular sympathetic nerve fibers course through the adventitia and release norepinephrine to stimulate SMCs via α-adrenoreceptors to produce contraction. In turn, SMCs can signal ECs through MEGJs to activate K+ channels and attenuate sympathetic vasoconstriction. Activation of K+ channels along the endothelium will dissipate electrical signal transmission and inhibit AVD, thereby restricting blood flow into the microcirculation while maintaining peripheral resistance and perfusion pressure. This review explores the origins and nature of intercellular signaling governing blood flow control in skeletal muscle with respect to the interplay between AVD and sympathetic innervation. Whereas these interactions are integral to physical daily activity and athletic performance, resolving the interplay between respective signaling events provides insight into how selective interventions can improve tissue perfusion and oxygen delivery during vascular disease. PMID:26368324

  1. Development of an Actuator for Flow Control Utilizing Detonation

    NASA Technical Reports Server (NTRS)

    Lonneman, Patrick J.; Cutler, Andrew D.

    2004-01-01

    Active flow control devices including mass injection systems and zero-net-mass flux actuators (synthetic jets) have been employed to delay flow separation. These devices are capable of interacting with low-speed, subsonic flows, but situations exist where a stronger crossflow interaction is needed. Small actuators that utilize detonation of premixed fuel and oxidizer should be capable of producing supersonic exit jet velocities. An actuator producing exit velocities of this magnitude should provide a more significant interaction with transonic and supersonic crossflows. This concept would be applicable to airfoils on high-speed aircraft as well as inlet and diffuser flow control. The present work consists of the development of a detonation actuator capable of producing a detonation in a single shot (one cycle). Multiple actuator configurations, initial fill pressures, oxidizers, equivalence ratios, ignition energies, and the addition of a turbulence generating device were considered experimentally and computationally. It was found that increased initial fill pressures and the addition of a turbulence generator aided in the detonation process. The actuators successfully produced Chapman-Jouguet detonations and wave speeds on the order of 3000 m/s.

  2. Ladle Shroud as a Flow Control Device for Tundish Operations

    NASA Astrophysics Data System (ADS)

    Morales-Higa, Ken; Guthrie, R. I. L.; Isac, M.; Morales, R. D.

    2013-02-01

    The performance characteristics of a tundish, such as the flotation of inclusions and slag entrainment, are largely influenced by the fluid-flow phenomena. Physical modeling in water is widely used to understand the fluid flows in a tundish and as a tool to improve, control, and design procedures for high-quality steel processing operations. These approaches were used to study the performance of fluid flow for a new design of ladle shroud. The new design for a dissipative ladle shroud (DLS) was studied, using a one-third scale, delta shaped, four-strand tundish. The results were compared with those achieved with the conventional ladle shroud. Different cases have been analyzed, including a conventional ladle shroud (LS) with a bare tundish and a tundish furnished with an impact pad. Similarly, the new design of the shroud (DLS) was studied under equivalent conditions. The physical experiments included the use of particle image velocimetry (PIV) and conductivity tracer techniques. The PIV measured the instantaneous velocities at the outlet of the DLS and the LS at different flow rates, showing the detailed jetting characteristics of water leaving the two types of ladle shroud. Residence time distribution (RTD) curves were also obtained for the different flow arrangements previously mentioned, and the dispersion of a colored dye tracer was observed at different intervals of time during tundish operation and analyzed using the video visualization technique.

  3. Formation of surface nanodroplets under controlled flow conditions

    NASA Astrophysics Data System (ADS)

    Lohse, Detlef; Zhang, Xuehua; Lu, Ziyang; Tan, Huanshu; Bao, Lei; He, Yinghe; Sun, Chao

    2015-11-01

    Nanodroplets on a solid surface (i.e. surface nanodroplets) have practical implications for high-throughput chemical and biological analysis, lubrications, lab-on-chip devices, and near-field imaging techniques. Oil nanodroplets can be produced on a solid-liquid interface in a simple step of solvent exchange in which a good solvent of oil is displaced by a poor solvent. In this work, we experimentally and theoretically investigate the formation of nanodroplets by the solvent exchange process under well-controlled flow conditions. We find significant effects from the flow rate and the flow geometry on the droplet size. We develop a theoretical framework to account for these effects. The main idea is that the droplet nuclei are exposed to an oil oversaturation pulse during the exchange process. The analysis gives that the volume of the nanodroplets increases with the Peclet number Pe of the flow as ~ Pe 3 / 4 , which is in good agreement with our experimental results. In addition, at fixed flow rate and thus fixed Peclet number, larger and less homogeneously distributed droplets formed at less narrow channels, due to convection effects originating from the density difference between the two solutions of the solvent exchange.

  4. Aerodynamic characteristics of missile control fins in nonlinear flow fields

    NASA Technical Reports Server (NTRS)

    Hemsch, M. J.; Nielsen, J. N.

    1983-01-01

    Recent experimental results show that the control effectiveness of a missile fin in supersonic flow at moderate-to-high angles of attack is a strong nonlinear function of free-stream Mach number, body incidence angle, fin bank angle and fin deflection angle. Analysis of the experimental results using an Euler finite-difference computer code with flow separation together with the equivalent angle-of-attack concept indicates that the observed nonlinearities are due to the variation of local dynamic pressure and local Mach number around the missile body alone. The nonlinearities are shown to be a strong source of control cross-coupling for high Mach number, high angle-of-attack combinations. The analysis suggests a relatively simple yet comprehensive approach for accurately accounting for these nonlinear effects.

  5. Reliable multicast protocol specifications flow control and NACK policy

    NASA Technical Reports Server (NTRS)

    Callahan, John R.; Montgomery, Todd L.; Whetten, Brian

    1995-01-01

    This appendix presents the flow and congestion control schemes recommended for RMP and a NACK policy based on the whiteboard tool. Because RMP uses a primarily NACK based error detection scheme, there is no direct feedback path through which receivers can signal losses through low buffer space or congestion. Reliable multicast protocols also suffer from the fact that throughput for a multicast group must be divided among the members of the group. This division is usually very dynamic in nature and therefore does not lend itself well to a priori determination. These facts have led the flow and congestion control schemes of RMP to be made completely orthogonal to the protocol specification. This allows several differing schemes to be used in different environments to produce the best results. As a default, a modified sliding window scheme based on previous algorithms are suggested and described below.

  6. Development of laminar flow control wing surface porous structure

    NASA Technical Reports Server (NTRS)

    Klotzsche, M.; Pearce, W.; Anderson, C.; Thelander, J.; Boronow, W.; Gallimore, F.; Brown, W.; Matsuo, T.; Christensen, J.; Primavera, G.

    1984-01-01

    It was concluded that the chordwise air collection method, which actually combines chordwise and spanwise air collection, is the best of the designs conceived up to this time for full chord laminar flow control (LFC). Its shallower ducting improved structural efficiency of the main wing box resulting in a reduction in wing weight, and it provided continuous support of the chordwise panel joints, better matching of suction and clearing airflow requirements, and simplified duct to suction source minifolding. Laminar flow control on both the upper and lower surfaces was previously reduced to LFC suction on the upper surface only, back to 85 percent chord. The study concludes that, in addition to reduced wing area and other practical advantages, this system would be lighter because of the increase in effective structural wing thickness.

  7. Lithium mass flow control for high power Lorentz Force Accelerators

    NASA Astrophysics Data System (ADS)

    Kodys, Andrea D.; Emsellem, Gregory; Cassady, Leonard D.; Polk, James E.; Choueiri, Edgar Y.

    2001-02-01

    A lithium feeding system has been developed to measure and control propellant flow for 30-200 kW Lithium Lorentz Force Accelerators (LiLFAs). The new, mechanically actuated, liquid lithium feed system has been designed and tested as a central component of a campaign to obtain basic data and establish scaling laws and performance relations for these thrusters. Calibration data are presented which demonstrate reliable and controllable feed of liquid lithium to the vaporizer hollow cathode of the thruster at flow rates between 10 and 120 mg/s. The ability to thermally track the liquid lithium through the system by the use of external temperature measurements is demonstrated. In addition, recent developments are presented in the establishment and successful testing of a lithium handling facility and safety procedures allowing for the in-house loading of the feed system and the neutralization, cleaning and disposal of up to 300 g of lithium. .

  8. Shuttle Gaseous Hydrogen Venting Risk from Flow Control Valve Failure

    NASA Technical Reports Server (NTRS)

    Drummond, J. Philip; Baurle, Robert A.; Gafney, Richard L.; Norris, Andrew T.; Pellett, Gerald L.; Rock, Kenneth E.

    2009-01-01

    This paper describes a series of studies to assess the potential risk associated with the failure of one of three gaseous hydrogen flow control valves in the orbiter's main propulsion system during the launch of Shuttle Endeavour (STS-126) in November 2008. The studies focused on critical issues associated with the possibility of combustion resulting from release of gaseous hydrogen from the external tank into the atmosphere during assent. The Shuttle Program currently assumes hydrogen venting from the external tank will result in a critical failure. The current effort was conducted to increase understanding of the risk associated with venting hydrogen given the flow control valve failure scenarios being considered in the Integrated In-Flight Anomaly Investigation being conducted by NASA.

  9. Tracking low SNR targets using particle filter with flow control

    NASA Astrophysics Data System (ADS)

    Moshtagh, Nima; Romberg, Paul M.; Chan, Moses W.

    2014-06-01

    In this work we study the problem of detecting and tracking challenging targets that exhibit low signal-to-noise ratios (SNR). We have developed a particle filter-based track-before-detect (TBD) algorithm for tracking such dim targets. The approach incorporates the most recent state estimates to control the particle flow accounting for target dynamics. The flow control enables accumulation of signal information over time to compensate for target motion. The performance of this approach is evaluated using a sensitivity analysis based on varying target speed and SNR values. This analysis was conducted using high-fidelity sensor and target modeling in realistic scenarios. Our results show that the proposed TBD algorithm is capable of tracking targets in cluttered images with SNR values much less than one.

  10. The Functional Activity of the Human Serotonin 5-HT1A Receptor Is Controlled by Lipid Bilayer Composition.

    PubMed

    Gutierrez, M Gertrude; Mansfield, Kylee S; Malmstadt, Noah

    2016-06-01

    Although the properties of the cell plasma membrane lipid bilayer are broadly understood to affect integral membrane proteins, details of these interactions are poorly understood. This is particularly the case for the large family of G protein-coupled receptors (GPCRs). Here, we examine the lipid dependence of the human serotonin 5-HT1A receptor, a GPCR that is central to neuronal function. We incorporate the protein in synthetic bilayers of controlled composition together with a fluorescent reporting system that detects GPCR-catalyzed activation of G protein to measure receptor-catalyzed oligonucleotide exchange. Our results show that increased membrane order induced by sterols and sphingomyelin increases receptor-catalyzed oligonucleotide exchange. Increasing membrane elastic curvature stress also increases this exchange. These results reveal the broad dependence that the 5-HT1A receptor has on plasma membrane properties, demonstrating that membrane lipid composition is a biochemical control parameter and highlighting the possibility that compositional changes related to aging, diet, or disease could impact cell signaling functions. PMID:27276266

  11. Mangiferin supplementation improves serum lipid profiles in overweight patients with hyperlipidemia: a double-blind randomized controlled trial

    PubMed Central

    Na, Lixin; Zhang, Qiao; Jiang, Shuo; Du, Shanshan; Zhang, Wei; Li, Ying; Sun, Changhao; Niu, Yucun

    2015-01-01

    Our previous studies have shown that mangiferin decreased serum triglycerides and free fatty acids (FFAs) by increasing FFAs oxidation in both animal and cell experiments. This study sought to evaluate the effects of mangiferin on serum lipid profiles in overweight patients with hyperlipidemia. Overweight patients with hyperlipidemia (serum triglyceride ≥ 1.70 mmol/L, and total cholesterol ≥ 5.2 mmol/L) were included in this double-blind randomized controlled trial. Participants were randomly allocated to groups, either receiving mangiferin (150 mg/day) or identical placebo for 12 weeks. The lipid profile and serum levels of mangiferin, glucose, L-carnitine, β-hydroxybutyrate, and acetoacetate were determined at baseline and 12 weeks. A total of 97 participants completed the trial. Compared with the placebo control, mangiferin supplementation significantly decreased the serum levels of triglycerides and FFAs, and insulin resistance index. Mangiferin supplementation also significantly increased the serum levels of mangiferin, high-density lipoprotein cholesterol, L-carnitine, β-hydroxybutyrate, and acetoacetate, and increased lipoprotein lipase activity. However, there were no differences in the serum levels of total cholesterol, low-density lipoprotein cholesterol, serum glucose, and insulin between groups. Mangiferin supplementation could improve serum lipid profiles by reducing serum triglycerides and FFAs in overweight patients with hyperlipidemia, partly due to the promotion of FFAs oxidation. PMID:25989216

  12. Randomised controlled trial of effect of fruit and vegetable consumption on plasma concentrations of lipids and antioxidants.

    PubMed Central

    Zino, S.; Skeaff, M.; Williams, S.; Mann, J.

    1997-01-01

    OBJECTIVES: To determine the extent to which plasma antioxidant concentrations in people with habitual low intake of fruit and vegetables respond to increased intakes of these foods. To examine whether advice to increase fruit and vegetables will result in reduction of concentrations of total and low density lipoprotein cholesterol. DESIGN: Randomised controlled trial in which intervention and control groups were followed up for eight weeks. The intervention group was asked to consume eight servings of fruit and vegetables a day. SETTING: Dunedin, New Zealand. SUBJECTS: Eighty seven subjects with normal lipid concentrations who ate three or fewer servings of fruit and vegetables daily. MAIN OUTCOME MEASURES: Plasma concentrations of vitamin C, retinol, alpha and beta carotene, alpha tocopherol, lipids, and lipoproteins. Dietary intake assessed with diet records over four days. RESULTS: The mean plasma vitamin C, alpha carotene, and beta carotene concentrations increased in parallel with increased dietary intake of fruit and vegetables in the intervention group. Concentrations of retinol, alpha tocopherol, lipids, and lipoproteins remained unchanged despite some increase in dietary vitamin E and a small reduction in saturated fat intake. CONCLUSIONS: Following a recommendation to increase fruit and vegetable consumption produces change in plasma concentrations of vitamin C, alpha carotene, and beta carotene likely to reduce incidence of cancer. More specific dietary advice to modify fat intake may be necessary to reduce the risk of cardiovascular disease mediated by lipoprotein and vitamin E. PMID:9224079

  13. Ground vibration test of the laminar flow control JStar airplane

    NASA Technical Reports Server (NTRS)

    Kehoe, M. W.; Cazier, F. W., Jr.; Ellison, J. F.

    1985-01-01

    A ground vibration test was conducted on a Lockheed JetStar airplane that had been modified for the purpose of conducting laminar flow control experiments. The test was performed prior to initial flight flutter tests. Both sine-dwell and single-point-random excitation methods were used. The data presented include frequency response functions and a comparison of mode frequencies and mode shapes from both methods.

  14. Dynamic control of aerodynamic forces on a moving platform using active flow control

    NASA Astrophysics Data System (ADS)

    Brzozowski, Daniel P.

    The unsteady interaction between trailing edge aerodynamic flow control and airfoil motion in pitch and plunge is investigated in wind tunnel experiments using a two degree-of-freedom traverse which enables application of time-dependent external torque and forces by servo motors. The global aerodynamic forces and moments are regulated by controlling vorticity generation and accumulation near the trailing edge of the airfoil using hybrid synthetic jet actuators. The dynamic coupling between the actuation and the time-dependent flow field is characterized using simultaneous force and particle image velocimetry (PIV) measurements that are taken phase-locked to the commanded actuation waveform. The effect of the unsteady motion on the model-embedded flow control is assessed in both trajectory tracking and disturbance rejection maneuvers. The time-varying aerodynamic lift and pitching moment are estimated from a PIV wake survey using a reduced order model based on classical unsteady aerodynamic theory. These measurements suggest that the entire flow over the airfoil readjusts within 2--3 convective time scales, which is about two orders of magnitude shorter than the characteristic time associated with the controlled maneuver of the wind tunnel model. This illustrates that flow-control actuation can be typically effected on time scales that are commensurate with the flow's convective time scale, and that the maneuver response is primarily limited by the inertia of the platform.

  15. Controls on matrix flow, preferential flow and deep drainage rates in an alluvial Vertisol.

    NASA Astrophysics Data System (ADS)

    Arnold, Sven; Larsen, Joshua; Reading, Lucy; Finch, Warren; Bulovic, Nevenka; McIntyre, Neil

    2016-04-01

    Deep drainage is the process that describes water percolating from the land surface to a depth below the root zone where it may contribute to groundwater recharge. Quantitative estimation of deep drainage through Vertisols is challenging, largely due to the unknown relative contributions from: (i) flow through the soil matrix; and (ii) flow along preferential pathways in particular soil cracks, and how to model the transience of the relative contributions. The Condamine River Alluvium, a significant aquifer in semi-arid eastern Australia, is mostly covered by uniform dark cracking clays such as Black and Grey Vertisols. The aim of this study was to identify the environmental conditions (rainfall, antecedent soil moisture, etc) controlling matrix and preferential flow in selected Vertisol profiles at the time scale of individual rainfall events. Field experiments (including 16 probes recording soil moisture at one hour intervals across eight depths between 100 mm and 4000 mm) provide extensive soil moisture data, supplemented by weather station data collected at 15-minute intervals. In addition, laboratory experiments were used to infer the water retention curves. These data were used to (i) derive deep drainage rates using the zero-flux plane method, and (ii) calibrate a soil moisture balance model that represents both matrix and preferential flow. The model was used to estimate the parts of the vertical water flux attributed to soil matrix and preferential flow. High antecedent soil moisture was associated with low fluxes at shallow depths, however at deeper depths both low and high antecedent soil moisture were associated with larger fluxes. Further, both rainfall amount and intensity controlled the interplay between matrix and preferential flow. The results reveal new insights into deep drainage processes in Vertisols and provide the basis for developing a practical approach for deep drainage estimation.

  16. Temperature-gated thermal rectifier for active heat flow control.

    PubMed

    Zhu, Jia; Hippalgaonkar, Kedar; Shen, Sheng; Wang, Kevin; Abate, Yohannes; Lee, Sangwook; Wu, Junqiao; Yin, Xiaobo; Majumdar, Arun; Zhang, Xiang

    2014-08-13

    Active heat flow control is essential for broad applications of heating, cooling, and energy conversion. Like electronic devices developed for the control of electric power, it is very desirable to develop advanced all-thermal solid-state devices that actively control heat flow without consuming other forms of energy. Here we demonstrate temperature-gated thermal rectification using vanadium dioxide beams in which the environmental temperature actively modulates asymmetric heat flow. In this three terminal device, there are two switchable states, which can be regulated by global heating. In the "Rectifier" state, we observe up to 28% thermal rectification. In the "Resistor" state, the thermal rectification is significantly suppressed (<1%). To the best of our knowledge, this is the first demonstration of solid-state active-thermal devices with a large rectification in the Rectifier state. This temperature-gated rectifier can have substantial implications ranging from autonomous thermal management of heating and cooling systems to efficient thermal energy conversion and storage. PMID:25010206

  17. Aerodynamic Flow Control of a Moving Axisymmetric Platform

    NASA Astrophysics Data System (ADS)

    Lambert, Thomas J.; Vukasinovic, Bojan; Glezer, Ari

    2013-11-01

    Active fluidic control of induced aerodynamic forces and moments on a moving axisymmetric platform is investigated in wind tunnel experiments. Actuation is effected by controlled interactions between an azimuthal array of integrated synthetic jets with the cross flow to induce localized flow attachment domains over the aft end of the model and thereby alter the global aerodynamic forces and moments. The axisymmetric platform is wire-mounted on a 6 DOF traverse such that each of the eight mounting wires is connected to a servo motor with an in-line load cell for monitoring the wire tension. The desired platform motion is controlled in closed-loop by a laboratory computer. The effects of continuous and transitory actuation on the induced aerodynamic forces of the moving platform are investigated in detail using high-speed PIV. The time-dependent changes in the forces are explored for model maneuvering and stabilization. It is found that the actuation induces forces and moments that are on the order of the forces and moments of the baseline flow. These measurements agree with preliminary results on the stabilization of a model moving in a single DOF demonstrating the effectiveness of the actuation for trajectory stabilization. Supported by the ARO.

  18. Electrically heated particulate filter with zoned exhaust flow control

    DOEpatents

    Gonze, Eugene V [Pinckney, MI

    2012-06-26

    A system includes a particulate matter (PM) filter that includes X zones. An electrical heater includes Y heater segments that are associated with respective ones of the X zones. The electrical heater is arranged upstream from and proximate with the PM filter. A valve assembly includes Z sections that are associated with respective ones of the X zones. A control module adjusts flow through each of the Z sections during regeneration of the PM filter via control of the valve assembly. X, Y and Z are integers.

  19. Overview of active flow control at NASA Langley Research Center

    NASA Astrophysics Data System (ADS)

    Pack, LaTunia G.; Joslin, Ronald D.

    1998-06-01

    The paper summarizes active flow control projects currently underway at the NASA Langley Research Center. Technology development is being pursued within a multidisciplinary, cooperative approach, involving the classical disciplines of fluid mechanics, structural mechanics, material science, acoustics, and stability and control theory. Complementing the companion papers in this session, the present paper will focus on projects that have the goal of extending the state- of-the-art in the measurement, prediction, and control of unsteady, nonlinear aerodynamics. Toward this goal, innovative actuators, micro and macro sensors, and control strategies are considered for high payoff flow control applications. The target payoffs are outlined within each section below. Validation of the approaches range from bench-top experiments to wind-tunnel experiments to flight tests. Obtaining correlations for future actuator and sensor designs are implicit in the discussion. The products of the demonstration projects and design tool development from the fundamental NASA R and D level technology will then be transferred to the Applied Research components within NASA, DOD, and US Industry.

  20. Control of baculovirus gp64-induced syncytium formation by membrane lipid composition.

    PubMed Central

    Chernomordik, L; Leikina, E; Cho, M S; Zimmerberg, J

    1995-01-01

    We have investigated the effects of membrane lipid composition on biological membrane fusion triggered by low pH and mediated by the baculovirus envelope glycoprotein gp64. Lysolipids, either added exogenously or produced in situ by phospholipase A2 treatment of cell membranes, reversibly inhibited syncytium formation. Lysolipids also decreased the baculovirus infection rate. In contrast, oleic and arachidonic acids and monoolein promoted cell-cell fusion. Membrane lipid composition affected pH-independent processes which followed the low-pH-induced change in fusion protein conformation. Inhibition and promotion of membrane fusion by a number of lipids could not be explained by mere binding or incorporation into membranes, but rather was correlated with the effective molecular shape of exogenous lipids. Our data are consistent with the hypothesis that membrane fusion proceeds through highly bent membrane intermediates (stalks) having a net negative curvature. Consequently, inverted cone-shaped lysolipids inhibit and cone-shaped cis-unsaturated fatty acids promote stalk formation and, ultimately, membrane fusion. PMID:7707532

  1. Novel Controlled Release Polymer-Lipid Formulations Processed by Hot Melt Extrusion.

    PubMed

    Maniruzzaman, Mohammed; Islam, Muhammad T; Halsey, Sheelagh; Amin, Devyani; Douroumis, Dennis

    2016-02-01

    The aim of the study was to investigate the effect of novel polymer/lipid formulations on the dissolution rates of the water insoluble indomethacin (INM), co-processed by hot melt extrusion (HME). Formulations consisted of the hydrophilic hydroxypropyl methyl cellulose polymer (HPMCAS) and stearoyl macrogol-32 glycerides-Gelucire 50/13 (GLC) were processed with a twin screw extruder to produce solid dispersions. The extrudates characterized by X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC) and hot stage microscopy (HSM) indicated the presence of amorphous INM within the polymer/lipid matrices. In-line monitoring via near-infrared (NIR) spectroscopy revealed significant peak shifts indicating possible interactions and H-bonding formation between the drug and the polymer/lipid carriers. Furthermore, in vitro dissolution studies showed a synergistic effect of the polymer/lipid carrier with 2-h lag time in acidic media followed by enhanced INM dissolution rates at pH > 5.5. PMID:26689407

  2. Flow control of micro-ramps on supersonic forward-facing step flow

    NASA Astrophysics Data System (ADS)

    Qing-Hu, Zhang; Tao, Zhu; Shihe, Yi; Anping, Wu

    2016-05-01

    The effects of the micro-ramps on supersonic turbulent flow over a forward-facing step (FFS) was experimentally investigated in a supersonic low-noise wind tunnel at Mach number 3 using nano-tracer planar laser scattering (NPLS) and particle image velocimetry (PIV) techniques. High spatiotemporal resolution images and velocity fields of supersonic flow over the testing model were captured. The fine structures and their spatial evolutionary characteristics without and with the micro-ramps were revealed and compared. The large-scale structures generated by the micro-ramps can survive the downstream FFS flowfield. The micro-ramps control on the flow separation and the separation shock unsteadiness was investigated by PIV results. With the micro-ramps, the reduction in the range of the reversal flow zone in streamwise direction is 50% and the turbulence intensity is also reduced. Moreover, the reduction in the average separated region and in separation shock unsteadiness are 47% and 26%, respectively. The results indicate that the micro-ramps are effective in reducing the flow separation and the separation shock unsteadiness. Project supported by the National Natural Science Foundation of China (Grant Nos. 11172326 and 11502280).

  3. Effect of Tai Chi exercise on blood lipid profiles: a meta-analysis of randomized controlled trials

    PubMed Central

    Pan, Xiao-hong; Mahemuti, Amina; Zhang, Xue-hua; Wang, Ya-ping; Hu, Po; Jiang, Ju-bo; Xiang, Mei-xiang; Liu, Gang; Wang, Jian-an

    2016-01-01

    Objective: Studies have demonstrated that Tai Chi exercise improves blood lipid level with inconsistent results. A meta-analysis was conducted to quantify the effects of Tai Chi on blood lipid profiles in humans. Methods: We screened the databases of PubMed, EMBASE, Cochrane Library (Central), Web of Science, China National Knowledge Infrastructure (CNKI), Wanfang data, and Clinicaltrials.gov for randomized controlled trials with Physiotherapy Evidence Database (PEDro) score more than 3 points up to June 2015. Six studies involving 445 subjects were included. Most trials applied 12-week Tai Chi intervention courses. Results: In comparison with the control group, blood triglyceride (TG) level difference between follow-up and baseline was statistically significantly lower in the Tai Chi practicing group (weighted mean difference (WMD) −16.81 mg/dl; 95% confidence intervals (CI) −31.27 to −2.35 mg/dl; P=0.02). A trend to improving total cholesterol (TC) reduction was found with Tai Chi (WMD −7.96 mg/dl; 95% CI −17.30 to 1.39 mg/dl; P=0.10). However, no difference was found in blood low-density lipoprotein cholesterol (LDL-C) or high-density lipoprotein cholesterol (HDL-C). Conclusions: Tai Chi exercise lowered blood TG level with a trend to decrease blood TC level. Our data suggest that Tai Chi has the potential to implement meaningful blood lipid modification and serve as an adjunctive exercise modality. The relationship between Tai Chi exercise regimen and lipid profile change might have a scientific priority for future investigation. PMID:27487809

  4. Digital feedwater and recirculation flow control for GPUN Oyster Creek

    SciTech Connect

    Burjorjee, D. ); Gan, B. )

    1992-01-01

    This paper describes the digital system for feedwater and recirculation control that GPU Nuclear will be installing at Oyster Creek during its next outage - expected circa December 1992. The replacement was motivated by considerations of reliability and obsolescence - the analog equipment was aging and reaching the end of its useful life. The new system uses Atomic Energy of Canada Ltd.'s software platform running on dual, redundant, industrial-grade 386 computers with opto-isolated field input/output (I/O) accessed through a parallel bus. The feedwater controller controls three main feed regulating valves, two low flow regulating valves, and two block valves. The recirculation controller drives the five scoop positioners of the hydraulic couplers. The system also drives contacts that lock up the actuators on detecting an open circuit in their current loops.

  5. Noise, anti-noise and fluid flow control.

    PubMed

    Williams, J E Ffowcs

    2002-05-15

    This paper celebrates Thomas Young's discovery that wave interference was responsible for much that is known about light and colour. A substantial programme of work has been aimed at controlling the noise of aerodynamic flows. Much of that field can be explained in terms of interference and it is argued in this paper that the theoretical techniques for analysing noise can also be seen to rest on interference effects. Interference can change the character of wave fields to produce, out of well-ordered fields, wave systems quite different from the interfering wave elements. Lighthill's acoustic analogy is described as an example of this effect, an example in which the exact model of turbulence-generated noise is seen to consist of elementary interfering sound waves; waves that are sometimes heard in advance of their sources. The paper goes on to describe an emerging field of technology where sound is suppressed by superimposing on it a destructively interfering secondary sound; one designed and manufactured specifically for interference. That sound is known as anti-sound, or anti-noise when the sound is chaotic enough. Examples are then referred to where the noisy effect to be controlled is actually a disturbance of a linearly unstable system; a disturbance that is destroyed by destructive interference with a deliberately constructed antidote. The practical benefits of this kind of instability control are much greater and can even change the whole character of flows. It is argued that completely unnatural unstable conditions can be held with active controllers generating destructively interfering elements. Examples are given in which gravitational instability of stratified fluids can be prevented. The Kelvin-Helmholtz instability of shear flows can also be avoided by simple controls. Those are speculative examples of what might be possible in future developments of an interference effect, which has made anti-noise a useful technology. PMID:12804281

  6. The role of lipid profile in determining the risk of ischemic stroke in the elderly: a case-control study.

    PubMed

    Denti, Licia; Cecchetti, Alessandra; Annoni, Valentina; Merli, Maria Francesca; Ablondi, Fabrizio; Valenti, Giorgio

    2003-01-01

    In this study, we investigated the association of lipids with ischemic stroke and its different subtypes in elderly patients. In particular, lipid parameters not extensively investigated so far in previous case-control studies specifically focused in the old population, such as lipoprotein Lp (a) and Apoproteins AI (ApoAI) and B (ApoB), have been taken into account. Seventy nine patients (mean age 83 +/- 7.4, range 67-99), consecutively admitted to a Geriatric Ward between January 1998 and June 2000 with acute stroke (first event) were studied. A complete clinical and laboratory assessment, including neurological evaluation, head CT scan, carotid ultrasonography and ECG, was employed to define the clinical and etiologic stroke subtype, according to standardized criteria. Fasting blood samples were collected within 48 h from admission, for determination of total cholesterol (TC), triglycerides (TG), High Density Lipoprotein-cholesterol (HDL-C), Lp(a), ApoAI and ApoB; Low Density Lipoprotein-Cholesterol (LDL-C) was estimated by Friedwald formula. Eighty eight age and sex-matched outpatients, referred to the hospital for non-inflammatory disorders of joints and musculoskeletal system, served as controls. Patients showed HDL-C and HDL-C/ApoAI ratio significantly lower than controls, with higher LDL-C/HDL-C ratio. Analysis on quartiles of lipoprotein concentrations showed also a significant increase in odds of stroke for LDL-C concentrations over 100 mg/dl, in absence of a linear relationship between LDL-C levels and risk. Multiple logistic regression, adjusting for non-lipid risk factors for stroke, confirmed the independent association of low HDL-C and HDL-C/ApoAI with all strokes, as well as with each subtype. In conclusion, these data suggest that lipids give some contribution to stroke risk even in the elderly, with a more prevalent role for HDL than LDL, and that lipid profile assessment must be taken into account in estimating the individual risk of stroke. PMID

  7. Improving Diffusing S-duct Performance by Secondary Flow Control

    NASA Technical Reports Server (NTRS)

    Reichert, Bruce A.; Wendt, Bruce J.

    1994-01-01

    The objective of this research was to study ways to reduce inlet flow distortion (i.e., total pressure nonuniformity) and improve total pressure recovery in a diffusing S-duct. This was accomplished by controlling the development of secondary flows within the duct through the use of tapered-fin type vortex generators. Reported are results for the bare duct and seven different configurations of vortex generators. Data presented for each configuration include surface static pressure, surface flow visualization, and exit plane total pressure and transverse velocity. The performance of each configuration was assessed by calculating total pressure recovery and inlet distortion descriptors from the data and comparing them to the values for the bare duct. The best configuration tested reduced distortion (as measured by the DC(45) and DC(90) descriptors) by more than 50 percent while improving total pressure recovery by 0.5 percent. These results should provide valuable guidance in designing vortex generator installations in ducts and for assessing the accuracy of computational fluid dynamics (CFD) methods to calculate duct flows with installed vortex generators.

  8. Optimization and Control of Acoustic Liner Impedance with Bias Flow

    NASA Technical Reports Server (NTRS)

    Wood, Houston; Follet, Jesse

    2000-01-01

    Because communities are impacted by steady increases in aircraft traffic, aircraft noise continues to be a growing problem for the growth of commercial aviation. Research has focused on improving the design of specific high noise source areas of aircraft and on noise control measures to alleviate noise radiated from aircraft to the surrounding environment. Engine duct liners have long been a principal means of attenuating engine noise. The ability to control in-situ the acoustic impedance of a liner would provide a valuable tool to improve the performance of liners. The acoustic impedance of a liner is directly related to the sound absorption qualities of that liner. Increased attenuation rates, the ability to change liner acoustic impedance to match various operating conditions, or the ability to tune a liner to more precisely match design impedance represent some ways that in-situ impedance control could be useful. With this in mind, the research to be investigated will focus on improvements in the ability to control liner impedance using a mean flow through the liner which is referred to as bias flow.

  9. Synthetic Jets Flow Control on a vertical stabilizer

    NASA Astrophysics Data System (ADS)

    Rathay, Nicholas; Boucher, Matthew; Amitay, Michael

    2011-11-01

    The vertical stabilizer on most commercial transport aircraft is much larger than required for stability and control. The tail is significantly oversized in order to maintain controllability in the event of asymmetric engine failure and meet flying qualities requirements related to dynamic motion. Using aerodynamic flow control techniques, it may be possible to reduce the size of the tail while maintaining similar control authority during inclement flight conditions. Reducing the size of the tail decreases the weight and the drag of the airplane, which results in considerable savings in fuel costs. In this work, it is shown that synthetic jet (zero-net-mass-flux) actuators are capable of reattaching the separated flow on the rudder and augmenting the performance of the stabilizer. Experiments were conducted in an open-return wind tunnel on a 1/25th scale model of a vertical stabilizer and a partial fuselage section. The surface pressure, aerodynamic loads and data acquired with a Stereo PIV system were used to investigate the effectiveness of this technology as well as provide a more detailed analysis of the flowfield and showed that the synthetic jets are capable of augmenting the side-force by up to 20%.

  10. Feedback control of flow alignment in sheared liquid crystals.

    PubMed

    Strehober, David A; Schöll, Eckehard; Klapp, Sabine H L

    2013-12-01

    Based on a continuum theory, we investigate the manipulation of the nonequilibrium behavior of a sheared liquid crystal via closed-loop feedback control. Our goal is to stabilize a specific dynamical state, that is, the stationary "flow alignment," under conditions where the uncontrolled system displays oscillatory director dynamics with in-plane symmetry. To this end we employ time-delayed feedback control (TDFC), where the equation of motion for the ith component q(i)(t) of the order parameter tensor is supplemented by a control term involving the difference q(i)(t)-q(i)(t-τ). In this diagonal scheme, τ is the delay time. We demonstrate that the TDFC method successfully stabilizes flow alignment for suitable values of the control strength K and τ; these values are determined by solving an exact eigenvalue equation. Moreover, our results show that only small values of K are needed when the system is sheared from an isotropic equilibrium state, contrary to the case where the equilibrium state is nematic. PMID:24483470

  11. Feedback control of flow alignment in sheared liquid crystals

    NASA Astrophysics Data System (ADS)

    Strehober, David A.; Schöll, Eckehard; Klapp, Sabine H. L.

    2013-12-01

    Based on a continuum theory, we investigate the manipulation of the nonequilibrium behavior of a sheared liquid crystal via closed-loop feedback control. Our goal is to stabilize a specific dynamical state, that is, the stationary "flow alignment," under conditions where the uncontrolled system displays oscillatory director dynamics with in-plane symmetry. To this end we employ time-delayed feedback control (TDFC), where the equation of motion for the ith component qi(t) of the order parameter tensor is supplemented by a control term involving the difference qi(t)-qi(t-τ). In this diagonal scheme, τ is the delay time. We demonstrate that the TDFC method successfully stabilizes flow alignment for suitable values of the control strength K and τ; these values are determined by solving an exact eigenvalue equation. Moreover, our results show that only small values of K are needed when the system is sheared from an isotropic equilibrium state, contrary to the case where the equilibrium state is nematic.

  12. Snapshot of Active Flow Control Research at NASA Langley

    NASA Technical Reports Server (NTRS)

    Washburn, A. E.; Gorton, S. Althoff; Anders, S. G.

    2002-01-01

    NASA Langley is aggressively investigating the potential advantages of active flow control as opposed to more traditional aerodynamic techniques. Many of these techniques will be blended with advanced materials and structures to further enhance payoff. Therefore a multi-disciplinary approach to technology development is being attempted that includes researchers from the more historical disciplines of fluid mechanics. acoustics, material science, structural mechanics, and control theory. The overall goals of the topics presented are focused on advancing the state of knowledge and understanding of controllable fundamental mechanisms in fluids rather than on specific engineering problems. An organizational view of current research activities at NASA Langley in active flow control as supported by several programs such as the Morphing Project under Breakthrough Vehicle Technologies Program (BVT). the Ultra-Efficient Engine Technology Program (UEET), and the 21st Century Aircraft Technology Program (TCAT) is presented. On-center research as well as NASA Langley funded contracts and grants are discussed at a relatively high level. The products of this research, as part of the fundamental NASA R and D (research and development) program. will be demonstrated as either bench-top experiments, wind-tunnel investigations, or in flight tests. Later they will be transferred to more applied research programs within NASA, DOD (Department of Defense), and U.S. industry.

  13. Passive jet control of flow around a circular cylinder

    NASA Astrophysics Data System (ADS)

    Chen, Wen-Li; Gao, Dong-Lai; Yuan, Wen-Yong; Li, Hui; Hu, Hui

    2015-11-01

    In the present study, a passive flow control method, which is featured by passive windward suction combined with leeward jet over a circular cylinder for drag reduction and dynamic wind loading suppression, was experimentally investigated to manipulate unsteady wake vortex shedding from a circular cylinder. Four perforated pipe designs with different numbers of suction/jet holes (i.e., from 2 to 24 suction/jet holes) were used to create flow communicating channels between the windward and leeward stagnation points of a cylindrical test model. The experimental study was performed in a wind tunnel at a Reynolds number of Re = 4.16 × 104 based on the cylinder diameter and oncoming airflow speed. In addition to measuring surface pressure distributions to determine the dynamic wind loads acting on the test model, a digital particle image velocimetry (PIV) system was also used to quantify the wake flow characteristics in order to assess the effectiveness of the passive jet control method with different perforated pipe designs, in comparison with a baseline case without passive jet control. It was found that the passive jet control method is very effective in manipulating the wake vortex shedding process from the circular cylinder. The perforated pipe designs with more suction/jet holes were found to be more effective in reducing drag and suppressing fluctuating amplitude of the dynamic wind loads acting on the test model. With 24 suction/jet holes evenly distributed over the cylindrical test model (i.e., the N13 design of the present study), the passive jet control method was found to be able to achieve up to 33.7 % in drag reduction and 90.6 % in fluctuating wind loading suppression, in comparison with the baseline case. The PIV measurement results revealed clearly that the passive jet control method would cause airflow jets into the cylinder wake and change the shedding modes of the wake vortex structures from the cylindrical test model. Because of the dynamic

  14. Flow Control Analysis on the Hump Model with RANS Tools

    NASA Technical Reports Server (NTRS)

    Viken, Sally A.; Vatsa, Veer N.; Rumsey, Christopher L.; Carpenter, Mark H.

    2003-01-01

    A concerted effort is underway at NASA Langley Research Center to create a benchmark for Computational Fluid Dynamic (CFD) codes. both unstructured and structured, against a data set for the hump model with actuation. The hump model was tested in the NASA Langley 0.3-m Transonic Cryogenic Tunnel. The CFD codes used for the analyses are the FUN2D (Full Unstructured Navier-Stokes 2-Dimensional) code, the structured TLNS3D (Thin-Layer Navier-Stokes 3-Dimensional) code, and the structured CFL3D code, all developed at NASA Langley. The current investigation uses the time-accurate Reynolds-Averaged Navier-Stokes (RANS) approach to predict aerodynamic performance of the active flow control experimental database for the hump model. Two-dimensional computational results verified that steady blowing and suction and oscillatory suction/blowing can be used to significantly reduce the separated flow region on the model. Discrepancies do exist between the CFD results and experimental data in the region downstream of the slot with the largest differences in the oscillatory cases. Overall, the structured CFD codes exhibited similar behavior with each other for a wide range of control conditions, with the unstructured FUN2D code showing moderately different results in the separated flow region for the suction and oscillatory cases.

  15. Development Activities on an Advanced Propellant Flow Control Unit

    NASA Astrophysics Data System (ADS)

    Noci, G.; Siciliano, P.; Fallerini, L.; Kutufa, N.; Rivetti, A.; Galassi, C.; Bruschi, P.; Piotto, M.

    2004-10-01

    A new generation of propellant control equipment for electric propulsion systems is needed in order to improve performance and operating ranges, symplify h/w configuration, reduce mass and dimensions, eliminate mass flow ripple, reduce time response. In this frame, the development of key components, their assembly and experimental investigation/ validation is on-going at Alenia Spazio-Laben/Business Unit Proel Tecnologie ( Proel in the following ) in the frame of an ESA GSTP program. The new components shall support different EP technologies, future EP multi-tasking capability and wide operating ranges. This paper reports about the development effort, its achievements and perspectives. 1. ABBREVIATIONS AND ACRONYMS BOL Beginning of Life CMBR Ceramic multilayer bender ring CTA Constant Temperature Anemometry. DUT Device under test EOL End of Life EP Electric Propulsion GEO Geosyncrhonous Earth Orbit GFCU Gas Flow Control Unit GIT Gridded ion thruster HET Hall Effect Thrusters LEO Low Earth Orbit LPC Low pressure capillary MEOP Maximum Expected Operating Pressure MFS Mass Flow rate Sensor NSSK North-South Station Keeping Pred Reduced pressure Ptank Tank pressure RMT Radiofrequency Magnetic Thruster RMTA Radiofrequency Magnetic Thruster Assembly ROOV Regulation and On-Off Valve SoW Statement of Work SPT Stationary Plasma Thruster.

  16. Development of fluidic oscillators as flow control actuators

    NASA Astrophysics Data System (ADS)

    Gregory, James Winborn

    This work is comprised of two key accomplishments: the study and design of fluidic oscillators for flow control applications, and the development and application of porous pressure-sensitive paint (PSP) for unsteady flowfields. PSP development was a necessary prerequisite for characterizing the unsteady fluid dynamics of the fluidic oscillators. Development work on the fluidic oscillator commences with a study on the internal fluid dynamics of the feedback-free class of oscillators. This study demonstrates that the collision of two jets within a mixing chamber forms an oscillating shear layer driven by counter-rotating vortices. A micro-scale version of this type of oscillator is also characterized with PSP measurements and frequency surveys. Subsequently, this high-frequency oscillator (˜ 5 kHz) is coupled with a low-frequency solenoid valve to create dual-frequency injection that is useful in flow control applications. A new hybrid actuator is developed that merges piezoelectric and fluidic technology. This piezo-fluidic oscillator successfully decouples the oscillation frequency from the supply pressure, thereby enabling closed-loop flow control actuation. Fluidic oscillators are then applied to a practical flow control application for cavity tone suppression. The fluidic oscillators are able to suppress the tone by 17.0 dB, while steady blowing at the same mass flow rate offers only 1.6-dB suppression. Work with pressure-sensitive paint involved development of a model for the quenching kinetics of the paint. Two fast-responding paint formulations, Polymer/ceramic and Fast FIB, are evaluated experimentally and compared to the model predictions. Both the model and experiments demonstrate that a paint layer will respond faster to a decrease in pressure than an increase of the same magnitude, and that the polymer/ceramic paint has a flat frequency response of at least 1.59 kHz. Furthermore, the excellent response characteristics of porous PSP are highlighted by

  17. Design and synthesis of lipidic organoalkoxysilanes for the self-assembly of liposomal nanohybrid cerasomes with controlled drug release properties.

    PubMed

    Liang, Xiaolong; Li, Xiaoda; Jing, Lijia; Xue, Peng; Jiang, Lingdong; Ren, Qiushi; Dai, Zhifei

    2013-11-18

    This paper reports the facile design and synthesis of a series of lipidic organoalkoxysilanes with different numbers of triethoxysilane headgroups and hydrophobic alkyl chains linked by glycerol and pentaerythritol for the construction of cerasomes with regulated surface siloxane density and controlled release behavior. It was found that the number of triethoxysilane headgroups affected the properties of the cerasomes for encapsulation efficiency, drug loading capacity, and release behavior. For both water-soluble doxorubicin (DOX) and water-insoluble paclitaxel (PTX), the release rate from the cerasomes decreased as the number of triethoxysilane headgroups increased. The slower release rate from the cerasomes was attributed to the higher density of the siloxane network on the surface of the cerasomes, which blocks the drug release channels. In contrast to the release results with DOX, the introduction of one more hydrophobic alkyl chain into the cerasome-forming lipid resulted in a slower release rate of PTX from the cerasomes due to the formation of a more compact cerasome bilayer. An MTT viability assay showed that all of these drug-loaded cerasomes inhibited proliferation of the HepG2 cancer cell line. The fine tuning of the chemical structure of the cerasome-forming lipids would foster a new strategy to precisely regulate the release rate of drugs from cerasomes. PMID:24123292

  18. Formation of size-controlled, denaturation-resistant lipid nanodiscs by an amphiphilic self-polymerizing peptide.

    PubMed

    Kondo, Hiroaki; Ikeda, Keisuke; Nakano, Minoru

    2016-10-01

    Nanodiscs are discoidal particles with a planar phospholipid bilayer enwrapped by proteins such as apolipoprotein A-I. Nanodiscs have been widely used for analyzing structures and functions of membrane proteins by dispersing them in solution. They are expected to be used as drug carriers and therapeutic agents. Amphiphilic peptides are known to form nanodiscs. However, the lipid-peptide nanodiscs are relatively unstable in solution, making them unsuitable for many applications. Here, we report the synthesis of an amphiphilic self-polymerizing peptide termed ASPP1, which polymerizes by intermolecular native chemical ligation reactions. ASPP1 spontaneously formed nanodiscs when added to phospholipid vesicles without using detergents. The diameter of the planar lipid bilayer in the nanodiscs was controlled by the lipid:peptide molar ratio. ASPP1-nanodiscs exhibited greater stability at high temperatures or in the presence of urea than nanodiscs formed by the non-polymerizing amphiphilic peptide or apolipoprotein A-I. Average and maximal degrees of ASPP1 polymerization were 2.4 and 12, respectively. Self-polymerization of the peptide appears to be responsible for stabilization of the nanodiscs. Our results open a new avenue for the development of nanodisc technology. PMID:27393815

  19. Dual-Responsive Lipid Nanotubes: Two-Way Morphology Control by pH and Redox Effects.

    PubMed

    Unsal, Hande; Schmidt, Judith; Talmon, Yeshayahu; Yildirim, Leyla Tatar; Aydogan, Nihal

    2016-05-31

    Lipid nanotubes are the preferred structures for many applications, especially biological ones, and thus have attracted much interest recently. However, there is still a significant need for developing more lipid nanotubes that are reversibly controllable to improve their functionality and usability. Here, we presented a two-way reversible morphology control of the nanotubes formed by the recently designed molecule AQUA (C25H29NO4). Because of its special design, the AQUA has both pH-sensitive and redox-active characters provided by the carboxylic acid and anthraquinone groups. Upon chemical reduction, the nanotubes turned into thinner ribbons, and this structural transformation was significantly reversible. The reduction of the AQUA nanotubes also switched the nanotubes from electrically conductive to insulative. Nanotube morphology can additionally be altered by decreasing the pH below the pKa value of the AQUA, at ∼4.9. Decreasing the pH caused the gradual unfolding of the nanotubes, and the interlayer distance in the nanotube's walls increased. This morphological change was fast and reversible at a wide pH range, including the physiological pH. Thus, the molecular design of the AQUA allowed for an unprecedented two-way and reversible morphology control with both redox and pH effects. These unique features make AQUA a very promising candidate for many applications, ranging from electronics to controlled drug delivery. PMID:27148756

  20. Choke for controlling the flow of drilling mud

    SciTech Connect

    Claycomb, J.R.

    1981-03-24

    This apparatus disclosed in its preferred form is a choke for controlling the flow of drilling mud which is highly abrasive. The abrasive drilling mud is introduced through a lateral port and an elongate, tubular body. In the tubular body, a fluid actuated, double acting piston is forced to a choke control position. The piston has appended to it a centralized, tubular extension which receives a sleeve of wear resistant material. The sleeve is positioned in a circular chamber communicated to the lateral port where the drilling mud is introduced. It travels toward a seat downstream of the body. The seat is formed of hardened material. The downstream outlet of the body is additionally lined with a wear resistant sleeve. An alternate embodiment is also disclosed. A hydraulic control system for operation of the choke is also disclosed.

  1. The automation of an inlet mass flow control system

    NASA Technical Reports Server (NTRS)

    Supplee, Frank; Tcheng, Ping; Weisenborn, Michael

    1989-01-01

    The automation of a closed-loop computer controlled system for the inlet mass flow system (IMFS) developed for a wind tunnel facility at Langley Research Center is presented. This new PC based control system is intended to replace the manual control system presently in use in order to fully automate the plug positioning of the IMFS during wind tunnel testing. Provision is also made for communication between the PC and a host-computer in order to allow total animation of the plug positioning and data acquisition during the complete sequence of predetermined plug locations. As extensive running time is programmed for the IMFS, this new automated system will save both manpower and tunnel running time.

  2. Speed limit and ramp meter control for traffic flow networks

    NASA Astrophysics Data System (ADS)

    Goatin, Paola; Göttlich, Simone; Kolb, Oliver

    2016-07-01

    The control of traffic flow can be related to different applications. In this work, a method to manage variable speed limits combined with coordinated ramp metering within the framework of the Lighthill-Whitham-Richards (LWR) network model is introduced. Following a 'first-discretize-then-optimize' approach, the first order optimality system is derived and the switch of speeds at certain fixed points in time is explained, together with the boundary control for the ramp metering. Sequential quadratic programming methods are used to solve the control problem numerically. For application purposes, experimental setups are presented wherein variable speed limits are used as a traffic guidance system to avoid traffic jams on highway interchanges and on-ramps.

  3. Computing an operating parameter of a unified power flow controller

    DOEpatents

    Wilson, David G; Robinett, III, Rush D

    2015-01-06

    A Unified Power Flow Controller described herein comprises a sensor that outputs at least one sensed condition, a processor that receives the at least one sensed condition, a memory that comprises control logic that is executable by the processor; and power electronics that comprise power storage, wherein the processor causes the power electronics to selectively cause the power storage to act as one of a power generator or a load based at least in part upon the at least one sensed condition output by the sensor and the control logic, and wherein at least one operating parameter of the power electronics is designed to facilitate maximal transmittal of electrical power generated at a variable power generation system to a grid system while meeting power constraints set forth by the electrical power grid.

  4. Computational Analysis of Cryogenic Flow Through a Control Valve

    NASA Technical Reports Server (NTRS)

    Danes, Russell; Woods, Jody; Sulyma, Peter

    2003-01-01

    The initial efforts to develop the capability to model valves used in rocket engine component testing at Stennis Space Center are documented. An axisymmetric model of a control valve with LN2 as the working fluid was developed. The goal was to predict the effect of change in the plug/sear region of the valve prior to testing. The valve flow coefficient was predicted for a range of plug positions. Verification of the calculations was carried out to quantify the uncertainty in the numerical answer. The modeled results compared well qualitatively to experimental trends. Additionally, insights into the flow processes in the valve were obtained. Benefits from the verification process included the ability to use coarser grids and insight into ways to reduce computational time by using double precision accuracy and non-integer grid ratios. Future valve modeling activities will include shape optimization of the valve/seat region and dynamic grid modeling.

  5. Stability theory applications to laminar-flow control

    NASA Technical Reports Server (NTRS)

    Malik, Mujeeb R.

    1987-01-01

    In order to design Laminar Flow Control (LFC) configurations, reliable methods are needed for boundary-layer transition predictions. Among the available methods, there are correlations based upon R sub e, shape factors, Goertler number and crossflow Reynolds number. The most advanced transition prediction method is based upon linear stability theory in the form of the e sup N method which has proven to be successful in predicting transition in two- and three-dimensional boundary layers. When transition occurs in a low disturbance environment, the e sup N method provides a viable design tool for transition prediction and LFC in both 2-D and 3-D subsonic/supersonic flows. This is true for transition dominated by either TS, crossflow, or Goertler instability. If Goertler/TS or crossflow/TS interaction is present, the e sup N will fail to predict transition. However, there is no evidence of such interaction at low amplitudes of Goertler and crossflow vortices.

  6. Aeroacoustics of Turbulent Jets: Flow Structure, Noise Sources, and Control

    NASA Astrophysics Data System (ADS)

    Gutmark, Ephraim Jeff; Callender, Bryan William; Martens, Steve

    The paper reviews research performed to advance the understanding of state-of-the-art technologies capable of reducing coaxial jet noise simulating the exhaust flow of turbofan engines. The review focuses on an emerging jet noise passive control technology known as chevron nozzles. The fundamental physical mechanisms responsible for the acoustic benefits provided by these nozzles are discussed. Additionally, the relationship between these physical mechanisms and some of the primary chevron geometric parameters are highlighted. Far-field acoustic measurements over a wide range of nozzle operating conditions illustrated the ability of the chevron nozzles to provide acoustic benefits. Detailed mappings of the acoustic near-field provided more insight into the chevron noise suppression mechanisms by successfully identifying two primary chevron effects consistent with the results of the far-field measurements: chevrons penetration and shear velocity across them. Mean and turbulence data identified the physical flow mechanisms responsible for the effects documented in the far- and near-field studies.

  7. Emission control valve with gas flow shut-off

    SciTech Connect

    Betterton, J.T.; Glover, A.H.; McKee, T.S.; Romanczuk, C.S.

    1990-03-06

    This patent describes, in an internal combustion engine, a crankcase gas flow control device located between the engine crankcase and the engine fuel-air induction. It comprises: a hollow housing, an apertured member supported at its outer edge by the housing. The apertured member forming an inlet and having an annular seating surface about the inlet aperture which faces the interior of the housing; a rod extending through the housing coaxially with the apertured inlet. The inlet forming member has a central boss portion engaging and supporting an end of the rod; a valve element in the housing and encircling the rod, the valve having a closed end normally seated against the seating surface to block gas flow through the inlet aperture; a coil type spring having one end axially fixed to the rod and another end engaging the valve element for yieldably urging the closed end against the seating surface.

  8. Systems control of BMP morphogen flow in vertebrate embryos

    PubMed Central

    Plouhinec, Jean-Louis; Zakin, Lise; De Robertis, Edward M.

    2011-01-01

    Embryonic morphogenetic programs coordinate cell behavior to ensure robust pattern formation. Having identified components of those programs by molecular genetics, developmental biology is now borrowing concepts and tools from systems biology to decode their regulatory logic. Dorsal-ventral (D-V) patterning of the frog gastrula by Bone Morphogenetic Proteins (BMPs) is one of the best studied examples of a self-regulating embryonic patterning system. Embryological analyses and mathematical modeling are revealing that the BMP activity gradient is maintained by a directed flow of BMP ligands towards the ventral side. Pattern robustness is ensured through feedback control of the levels of extracellular BMP pathway modulators that adjust the flow to the dimensions of the embryonic field. PMID:21937218

  9. Electromagnetic valve for controlling the flow of molten, magnetic material

    DOEpatents

    Richter, T.

    1998-06-16

    An electromagnetic valve for controlling the flow of molten, magnetic material is provided, which comprises an induction coil for generating a magnetic field in response to an applied alternating electrical current, a housing, and a refractory composite nozzle. The nozzle is comprised of an inner sleeve composed of an erosion resistant refractory material (e.g., a zirconia ceramic) through which molten, magnetic metal flows, a refractory outer shell, and an intermediate compressible refractory material, e.g., unset, high alumina, thermosetting mortar. The compressible refractory material is sandwiched between the inner sleeve and outer shell, and absorbs differential expansion stresses that develop within the nozzle due to extreme thermal gradients. The sandwiched layer of compressible refractory material prevents destructive cracks from developing in the refractory outer shell. 5 figs.

  10. Electromagnetic valve for controlling the flow of molten, magnetic material

    DOEpatents

    Richter, Tomas

    1998-01-01

    An electromagnetic valve for controlling the flow of molten, magnetic material is provided, which comprises an induction coil for generating a magnetic field in response to an applied alternating electrical current, a housing, and a refractory composite nozzle. The nozzle is comprised of an inner sleeve composed of an erosion resistant refractory material (e.g., a zirconia ceramic) through which molten, magnetic metal flows, a refractory outer shell, and an intermediate compressible refractory material, e.g., unset, high alumina, thermosetting mortar. The compressible refractory material is sandwiched between the inner sleeve and outer shell, and absorbs differential expansion stresses that develop within the nozzle due to extreme thermal gradients. The sandwiched layer of compressible refractory material prevents destructive cracks from developing in the refractory outer shell.

  11. Effect of Fructose on Established Lipid Targets: A Systematic Review and Meta-Analysis of Controlled Feeding Trials

    PubMed Central

    Chiavaroli, Laura; de Souza, Russell J; Ha, Vanessa; Cozma, Adrian I; Mirrahimi, Arash; Wang, David D; Yu, Matthew; Carleton, Amanda J; Di Buono, Marco; Jenkins, Alexandra L; Leiter, Lawrence A; Wolever, Thomas M S; Beyene, Joseph; Kendall, Cyril W C; Jenkins, David J A; Sievenpiper, John L

    2015-01-01

    Background Debate over the role of fructose in mediating cardiovascular risk remains active. To update the evidence on the effect of fructose on established therapeutic lipid targets for cardiovascular disease (low-density lipoprotein cholesterol [LDL]-C, apolipoprotein B, non-high-density lipoprotein cholesterol [HDL-C]), and metabolic syndrome (triglycerides and HDL-C), we conducted a systematic review and meta-analysis of controlled feeding trials. Methods and Results MEDLINE, EMBASE, CINHAL, and the Cochrane Library were searched through July 7, 2015 for controlled feeding trials with follow-up ≥7 days, which investigated the effect of oral fructose compared to a control carbohydrate on lipids (LDL-C, apolipoprotein B, non-HDL-C, triglycerides, and HDL-C) in participants of all health backgrounds. Two independent reviewers extracted relevant data. Data were pooled using random effects models and expressed as mean difference with 95% CI. Interstudy heterogeneity was assessed (Cochran Q statistic) and quantified (I2 statistic). Eligibility criteria were met by 51 isocaloric trials (n=943), in which fructose was provided in isocaloric exchange for other carbohydrates, and 8 hypercaloric trials (n=125), in which fructose supplemented control diets with excess calories compared to the control diets alone without the excess calories. Fructose had no effect on LDL-C, non-HDL-C, apolipoprotein B, triglycerides, or HDL-C in isocaloric trials. However, in hypercaloric trials, fructose increased apolipoprotein B (n=2 trials; mean difference = 0.18 mmol/L; 95% CI: 0.05, 0.30; P=0.005) and triglycerides (n=8 trials; mean difference = 0.26 mmol/L; 95% CI: 0.11, 0.41; P<0.001). The study is limited by small sample sizes, limited follow-up, and low quality scores of the included trials. Conclusions Pooled analyses showed that fructose only had an adverse effect on established lipid targets when added to existing diets so as to provide excess calories (+21% to 35% energy

  12. Molecular tubes for lipid sensing: tube conformations control analyte selectivity and fluorescent response.

    PubMed

    Avetta, Christopher T; Shorthill, Berkeley J; Ren, Chun; Glass, Timothy E

    2012-01-20

    Two fluorescent sensors for lipids have been prepared and tested for detection of a number of hydrophobic compounds of varying shape and size. The data suggest that the two sensors have a different mode of fluorescent response. Yet, the two sensors are only different in the bridging group--one having a flexible amide and one having a rigid allyl bridge. The fluorescence data are explained based on a difference in conformation of the two sensors in aqueous solution. PMID:22263717

  13. The Ultimate Flow Controlled Wind Turbine Blade Airfoil

    NASA Astrophysics Data System (ADS)

    Seifert, Avraham; Dolgopyat, Danny; Friedland, Ori; Shig, Lior

    2015-11-01

    Active flow control is being studied as an enabling technology to enhance and maintain high efficiency of wind turbine blades also with contaminated surface and unsteady winds as well as at off-design operating conditions. The study is focused on a 25% thick airfoil (DU91-W2-250) suitable for the mid blade radius location. Initially a clean airfoil was fabricated and tested, as well as compared to XFoil predictions. From these experiments, the evolution of the separation location was identified. Five locations for installing active flow control actuators are available on this airfoil. It uses both Piezo fluidic (``Synthetic jets'') and the Suction and Oscillatory Blowing (SaOB) actuators. Then we evaluate both actuation concepts overall energy efficiency and efficacy in controlling boundary layer separation. Since efficient actuation is to be found at low amplitudes when placed close to separation location, distributed actuation is used. Following the completion of the baseline studies the study has focused on the airfoil instrumentation and extensive wind tunnel testing over a Reynolds number range of 0.2 to 1.5 Million. Sample results will be presented and outline for continued study will be discussed.

  14. Ion channel networks in the control of cerebral blood flow.

    PubMed

    Longden, Thomas A; Hill-Eubanks, David C; Nelson, Mark T

    2016-03-01

    One hundred and twenty five years ago, Roy and Sherrington made the seminal observation that neuronal stimulation evokes an increase in cerebral blood flow.(1) Since this discovery, researchers have attempted to uncover how the cells of the neurovascular unit-neurons, astrocytes, vascular smooth muscle cells, vascular endothelial cells and pericytes-coordinate their activity to control this phenomenon. Recent work has revealed that ionic fluxes through a diverse array of ion channel species allow the cells of the neurovascular unit to engage in multicellular signaling processes that dictate local hemodynamics.In this review we center our discussion on two major themes: (1) the roles of ion channels in the dynamic modulation of parenchymal arteriole smooth muscle membrane potential, which is central to the control of arteriolar diameter and therefore must be harnessed to permit changes in downstream cerebral blood flow, and (2) the striking similarities in the ion channel complements employed in astrocytic endfeet and endothelial cells, enabling dual control of smooth muscle from either side of the blood-brain barrier. We conclude with a discussion of the emerging roles of pericyte and capillary endothelial cell ion channels in neurovascular coupling, which will provide fertile ground for future breakthroughs in the field. PMID:26661232

  15. Flow control for capillary-pumped microfluidic systems

    NASA Astrophysics Data System (ADS)

    Vestad, T.; Marr, D. W. M.; Oakey, J.

    2004-11-01

    Advantages of performing analytical and diagnostic tasks in microfluidic-based systems include small sample volume requirements, rapid transport times and the promise of compact, portable instrumentation. The application of such systems in home and point-of-care situations has been limited, however, because these devices typically require significant associated hardware to initiate and control fluid flow. Capillary-based pumping can address many of these deficiencies by taking advantage of surface tension to pull fluid through devices. The development of practical instrumentation however will rely upon the development of precision control schemes to complement capillary pumping. Here, we introduce a straightforward, robust approach that allows for reconfigurable fluid guidance through otherwise fixed capillary networks. This technique is based on the opening and closing of microfluidic channels cast in a flexible elastomer via automated or even manual mechanical actuation. This straightforward approach can completely and precisely control flows such as samples of complex fluids, including whole blood, at very high resolutions according to real-time user feedback. These results demonstrate the suitability of this technique for portable, microfluidic instruments in laboratory, field or clinical diagnostic applications.

  16. An instrument to control parallel plate separation for nanoscale flow control

    NASA Astrophysics Data System (ADS)

    White, J.; Ma, H.; Lang, J.; Slocum, A.

    2003-11-01

    The handling of extremely small samples of gases and liquids has long been a subject of research among biologists, chemists, and engineers. A few scientific instruments, notably the surface force apparatus, have been used extensively to investigate very short-range molecular phenomena. This article describes the design, fabrication, and characterization of an easily manufactured, gas and liquid flow control device called the Nanogate. The Nanogate controls liquid flows under very high planar confinement, wherein the liquid film is, in one dimension, on the scale of nanometers, but is on the scale of hundreds of microns in its other dimensions. The liquid film is confined between a silica (Pyrex) surface with a typical roughness of Ra≈6 nm and a gold-covered silicon surface with a typical roughness of Ra≈2 nm. During the manufacturing process, the Pyrex flows and conforms to the gold-covered silicon surface, improving the mating properties of the two surfaces. The fluid film thickness can be controlled within 2 Å, from sub-10 nm up to 1 μm. Control of helium gas flow rates in the 10-9 atm cm3/s range, and sub-nl/s flow rates of water and methanol have been predicted and experimentally verified.

  17. In-situ temperature-controllable shear flow device for neutron scattering measurement—An example of aligned bicellar mixtures

    SciTech Connect

    Xia, Yan; Li, Ming; Kučerka, Norbert; Li, Shutao; Nieh, Mu-Ping

    2015-02-15

    We have designed and constructed a temperature-controllable shear flow cell for in-situ study on flow alignable systems. The device has been tested in the neutron diffraction and has the potential to be applied in the small angle neutron scattering configuration to characterize the nanostructures of the materials under flow. The required sample amount is as small as 1 ml. The shear rate on the sample is controlled by the flow rate produced by an external pump and can potentially vary from 0.11 to 3.8 × 10{sup 5} s{sup −1}. Both unidirectional and oscillational flows are achievable by the setting of the pump. The instrument is validated by using a lipid bicellar mixture, which yields non-alignable nanodisc-like bicelles at low T and shear-alignable membranes at high T. Using the shear cell, the bicellar membranes can be aligned at 31 °C under the flow with a shear rate of 11.11 s{sup −1}. Multiple high-order Bragg peaks are observed and the full width at half maximum of the “rocking curve” around the Bragg’s condition is found to be 3.5°–4.1°. It is noteworthy that a portion of the membranes remains aligned even after the flow stops. Detailed and comprehensive intensity correction for the rocking curve has been derived based on the finite rectangular sample geometry and the absorption of the neutrons as a function of sample angle [See supplementary material at http://dx.doi.org/10.1063/1.4908165 for the detailed derivation of the absorption correction]. The device offers a new capability to study the conformational or orientational anisotropy of the solvated macromolecules or aggregates induced by the hydrodynamic interaction in a flow field.

  18. Control of High-Speed Spray Flows Using a Steady, Parallel Control Flow Under the Influence of the Coanda Effect

    NASA Astrophysics Data System (ADS)

    Allen, Dustin; Smith, Barton

    2007-11-01

    An experimental demonstration of a jet vectoring technique used in our novel spray device called a Coanda-assisted Spray Manipulation (CSM) nozzle is presented. The CSM makes use of a Coanda-like effect on axisymmetric geometries through the interaction of a high volume-flow primary jet flowing through the center of a collar and a secondary high-momentum jet parallel to the first and adjacent to a convex collar. The control jet attaches to the convex wall and vectors due to the Coanda effect, entraining and vectoring the primary jet, resulting in controllable r-theta directional spraying. Various annular secondary exit holes and curved wall radii were tested over a range of momentum flux ratios to study the effects of these variables on the vectored jet angle. Particle Image Velocimetry (PIV) was used to determine the vectoring angle and the profile of the primary jet in each experiment. The experiments show that the secondary exit hole size and curve wall radius, along with the momentum ratios of the two jets predominantly affect the vectoring angle of the primary jet. Also, the jet profile is largely unchanged with vectoring for high velocity flows, which is important for the thermal spray applications for which CSM will be used.

  19. Numerical investigations of turbulent flow past a rectangular cylinder with active flow control

    NASA Astrophysics Data System (ADS)

    Luong, Sanh B.

    The objective of the present research was to investigate the effects of rotating circular cylinders to control high intensity wind load. This research used computational fluid dynamics (CFD) to simulate high Reynolds number gust-like wind load condition for a transient duration of 12 seconds across a three-dimensional rectangular cylinder with dimension of 240x15x7 meters and aspect ratio (Breadth/Height) of 2.3. An array of 20 circular cylinders was positioned along the leading edges of the rectangular bridge cylinder. The research analyzed turbulent flow characteristics across the top and bottom deck surfaces and the development of wake region during two cases: 1) stationary cylinders and 2) rotated cylinders at 400 RPM or velocity ratio of lambda = 1.33. The Strouhal number flow characteristics of 0.08 and 0.17 for aspect ratio of 2 to 3 analyzed in this study were found to be in agreements with published literature.

  20. The aerodynamic performance of several flow control devices for internal flow systems

    NASA Technical Reports Server (NTRS)

    Eckert, W. T.; Wettlaufer, B. M.; Mort, K. W.

    1982-01-01

    An experimental reseach and development program was undertaken to develop and document new flow-control devices for use in the major modifications to the 40 by 80 Foot wind tunnel at Ames Research Center. These devices, which are applicable to other facilities as well, included grid-type and quasi-two-dimensional flow straighteners, louver panels for valving, and turning-vane cascades with net turning angles from 0 deg to 90 deg. The tests were conducted at model scale over a Reynolds number range from 2 x 100,000 to 17 x 100,000, based on chord. The results showed quantitatively the performance benefits of faired, low-blockage, smooth-surface straightener systems, and the advantages of curved turning-vanes with hinge-line gaps sealed and a preferred chord-to-gap ratio between 2.5 and 3.0 for 45 deg or 90 deg turns.

  1. 25. Typical valves used to control flow into and out ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. Typical valves used to control flow into and out of filtration bed. Left valve (painted red) drains the bed, and center valve (painted green) admits water into the bed. The right valve is a cross over valve which is used to admit water into a dry bed from the bottom. This bottom fill excludes entrapped air as the bed is filled. When the water reached to top of the bed, filling is continued from the top of the bed. - Lake Whitney Water Filtration Plant, Filtration Plant, South side of Armory Street between Edgehill Road & Whitney Avenue, Hamden, New Haven County, CT

  2. Development of Design Tools for Flow-Control Actuators

    NASA Technical Reports Server (NTRS)

    Mathew, Jose; Gallas, Quentin; Cattafesta, Louis N., III

    2003-01-01

    This report discusses the: 1. Development coupled electro/fluid/structural lumped-element model (LEM) of a prototypical flow-control actuator. 2. Validation the coupled electro/fluid/structural dynamics lumped-element models. 3. Development simple, yet effective, design tools for actuators. 4. Development structural dynamic models that accurately characterize the dynamic response of piezoelectric flap actuators using the Finite Element Method (FEW as well as analytical methods. 5. Perform a parametric study of a piezo-composite flap actuator. 6.Develop an optimization scheme for maximizing the actuator performance.

  3. Aircraft energy efficiency laminar flow control wing design study

    NASA Technical Reports Server (NTRS)

    Bonner, T. F., Jr.; Pride, J. D., Jr.; Fernald, W. W.

    1977-01-01

    An engineering design study was performed in which laminar flow control (LFC) was integrated into the wing of a commercial passenger transport aircraft. A baseline aircraft configuration was selected and the wing geometry was defined. The LFC system, with suction slots, ducting, and suction pumps was integrated with the wing structure. The use of standard aluminum technology and advanced superplastic formed diffusion bonded titanium technology was evaluated. The results of the design study show that the LFC system can be integrated with the wing structure to provide a structurally and aerodynamically efficient wing for a commercial transport aircraft.

  4. Balanced pan-PPAR activator bezafibrate in combination with statin: comprehensive lipids control and diabetes prevention?

    PubMed

    Tenenbaum, Alexander; Fisman, Enrique Z

    2012-01-01

    atherosclerotic plaque regression in thoracic and abdominal aorta and improved endothelial function. In addition, bezafibrate has important fibrinogen-related properties and anti-inflammatory effects. In clinical trials bezafibrate was highly effective for cardiovascular risk reduction in patients with metabolic syndrome and atherogenic dyslipidemia. The principal differences between bezafibrate and other fibrates are related to effects on glucose level and insulin resistance. Bezafibrate decreases blood glucose level, HbA1C, insulin resistance and reduces the incidence of T2DM compared to placebo or other fibrates. Currently statins are the cornerstone of the treatment and prevention of cardiovascular diseases related to atherosclerosis. However, despite the increasing use of statins as monotherapy for low density lipoprotein- cholesterol (LDL-C) reduction, a significant residual cardiovascular risk is still presented in patients with atherogenic dyslipidemia and insulin resistance, which is typical for T2DM and metabolic syndrome. Recently, concerns were raised regarding the development of diabetes in statin-treated patients. Combined bezafibrate/statin therapy is more effective in achieving a comprehensive lipid control and residual cardiovascular risk reduction. Based on the beneficial effects of pan-PPAR agonist bezafibrate on glucose metabolism and prevention of new-onset diabetes, one could expect a neutralization of the adverse pro-diabetic effect of statins using the strategy of a combined statin/fibrate therapy. PMID:23150952

  5. Kinetic study of the aggregation and lipid mixing produced by alpha-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements.

    PubMed Central

    Mancheño, J. M.; Gasset, M.; Lacadena, J.; Ramón, F.; Martínez del Pozo, A.; Oñaderra, M.; Gavilanes, J. G.

    1994-01-01

    alpha-Sarcin is a fungal cytotoxic protein that inactivates the eukaryotic ribosomes. A kinetic study of the aggregation and lipid mixing promoted by this protein on phosphatidylglycerol (PG) and phosphatidylserine (PS) vesicles has been performed. Egg yolk PG, bovine brain PS, dimyristoyl-PG (DMPG) and dimyristoyl-PS (DMPS) vesicles have been considered. The initial rates of the vesicle aggregation induced by the protein have been measured by stopped-flow 90 degrees light scattering. The formation of a vesicle dimer as the initial step of this process was deduced from the second-order dependence of the initial rates on phospholipid concentration. The highest alpha-sarcin concentration studied did not inhibit the vesicle aggregation, indicating that many protein molecules are involved in the vesicle cross-linking. These are common characteristics of the initial steps of the aggregation produced by alpha-sarcin in the four types of phospholipid vesicles considered. However, the kinetics of the scattering values revealed that more complex changes occurred in the later steps of the aggregation process of egg PG and brain PS vesicles than in those of their synthetic counterparts. alpha-Sarcin produced lipid mixing in vesicles composed of DMPG or DMPS, which was measured by fluorescence resonance energy transfer assays. A delay in the onset of the process, dependent on the protein concentration, was observed. Measurement of the rates of lipid mixing revealed that the process is first order on phospholipid concentration. Egg PG and brain PS vesicles did not show lipid mixing, although they avidly aggregated. However, alpha-sarcin was able to promote lipid mixing in heterogeneous systems composed of egg PG+DMPG or brain PS+DMPS vesicles. The dilution of the fluorescence probes was faster when these were incorporated into the bilayers made of synthetic phospholipids than were present in those made of natural phospholipids. The bilayer destabilization produced by the

  6. Evaluating vortex generator jet experiments for turbulent flow separation control

    NASA Astrophysics Data System (ADS)

    von Stillfried, F.; Kékesi, T.; Wallin, S.; Johansson, A. V.

    2011-12-01

    Separating turbulent boundary-layers can be energized by streamwise vortices from vortex generators (VG) that increase the near wall momentum as well as the overall mixing of the flow so that flow separation can be delayed or even prevented. In general, two different types of VGs exist: passive vane VGs (VVG) and active VG jets (VGJ). Even though VGs are already successfully used in engineering applications, it is still time-consuming and computationally expensive to include them in a numerical analysis. Fully resolved VGs in a computational mesh lead to a very high number of grid points and thus, computational costs. In addition, computational parameter studies for such flow control devices take much time to set-up. Therefore, much of the research work is still carried out experimentally. KTH Stockholm develops a novel VGJ model that makes it possible to only include the physical influence in terms of the additional stresses that originate from the VGJs without the need to locally refine the computational mesh. Such a modelling strategy enables fast VGJ parameter variations and optimization studies are easliy made possible. For that, VGJ experiments are evaluated in this contribution and results are used for developing a statistical VGJ model.

  7. Active flow control for a NACA-0012 profile

    NASA Astrophysics Data System (ADS)

    Oualli, H.; Mekadem, M.; Boukrif, M.; Saad, S.; Bouabdallah, A.; Gad-El-Hak, M.

    2015-11-01

    Active flow control is applied on a NACA-0012 profile. The experiments are carried out in a wind tunnel, and flow visualizations are conducted using high-resolution visible-light and infrared cameras. Numerical LES finite-volume code is used to complement the physical experiments. The symmetric wing is clipped into two parts, and those parts extend and retract along the chord according to the same sinusoidal law we optimized last year for a circular/elliptical cylinder (B. Am. Phys. Soc., vol. 59, no. 20, p. 319, 2014). The Reynolds number varies in the range of 500-100,000, which is typical of UAVs and micro-UAVs. The nascent cavity resulting from the oscillatory motion of the profile segments is kept open allowing the passage of fluid between the intrados and extrados. The pulsatile motion is characterized by an amplitude and frequency, and the airfoil's angle of attack is changed in the range of 0-30 deg. For certain amplitude and frequency, the drag coefficient is increased over the uncontrolled case by a factor of 300. But when the cavity is covered to prevent the flow from passing through the cavity, the drag coefficient becomes negative, and significant thrust is produced. The results are promising to achieve rapid deceleration and acceleration of UAVs.

  8. A novel control strategy for a Taylor-Couette flow

    NASA Astrophysics Data System (ADS)

    Bouabdallah, A.; Oualli, H.; Mekadem, M.; Boukrif, M.; Saad, S.; Gad-El-Hak, M.

    2015-11-01

    Advancing transition is desired in applications where heat, mass, or momentum transfer needs to be augmented. On the other hand, delaying transition is imperative in crystal growth devices, where all instabilities are to be avoided in order to prevent the appearance of geometrical irregularities in the resulting crystal. The hydrodynamic stability of a viscous flow in a closed, fully filled Taylor-Couette system is considered in the present numerical study. The fluid evolves in an annular cavity between the rotating inner cylinder and the outer fixed one. The base flow is axis-symmetric with two counter-rotating vortices each wavelength. The Taylor number varies in the range of 0-50. Numerical simulations are implemented on a finite-volume CFD code. The control strategy involves a pulsatile motion superimposed separately on the inner and outer cylinder's cross-section, with maximum amplitude of, respectively, 5% and 15% of the radius. The frequency varies in the range of 0-100 Hz. The objective is to localize the transition and to assess the flow's response to the imposed boundary motions. Substantial advancement of transition is found when the inner cylinder's cross-section is varied, while this transition is delayed when the outer cylinder's cross-section is pulsating.

  9. Stability analysis of traffic flow with extended CACC control models

    NASA Astrophysics Data System (ADS)

    Ya-Zhou, Zheng; Rong-Jun, Cheng; Siu-Ming, Lo; Hong-Xia, Ge

    2016-06-01

    To further investigate car-following behaviors in the cooperative adaptive cruise control (CACC) strategy, a comprehensive control system which can handle three traffic conditions to guarantee driving efficiency and safety is designed by using three CACC models. In this control system, some vital comprehensive information, such as multiple preceding cars’ speed differences and headway, variable safety distance (VSD) and time-delay effect on the traffic current and the jamming transition have been investigated via analytical or numerical methods. Local and string stability criterion for the velocity control (VC) model and gap control (GC) model are derived via linear stability theory. Numerical simulations are conducted to study the performance of the simulated traffic flow. The simulation results show that the VC model and GC model can improve driving efficiency and suppress traffic congestion. Project supported by the National Natural Science Foundation of China (Grant Nos. 71571107 and 11302110). The Scientific Research Fund of Zhejiang Province, China (Grant Nos. LY15A020007, LY15E080013, and LY16G010003). The Natural Science Foundation of Ningbo City (Grant Nos. 2014A610030 and 2015A610299), the Fund from the Government of the Hong Kong Administrative Region, China (Grant No. CityU11209614), and the K C Wong Magna Fund in Ningbo University, China.

  10. Dissipation-enhanced quartz crystal microbalance studies on the experimental parameters controlling the formation of supported lipid bilayers.

    PubMed

    Seantier, B; Breffa, C; Félix, O; Decher, G

    2005-11-24

    We report on the investigations of the transformation of spherically closed lipid bilayers to supported lipid bilayers in aqueous media in contact with SiO(2) surfaces. The adsorption kinetics of small unilamellar vesicles composed of dimyristoyl- (DMPC) and dipalmitoylphosphatidylcholine (DPPC) mixtures on SiO(2) surfaces were investigated using a dissipation-enhanced quartz crystal microbalance (QCM-D) as a function of buffer (composition and pH), lipid concentration (0.01-1.0 mg/mL), temperature (15-37 degrees C), and lipid composition (DMPC and DMPC/DPPC mixtures). The lipid mixtures used here possess a phase transition temperature (T(m)) of 24-33 degrees C, which is close to the ambient temperature or above and thus considerably higher than most other systems studied by QCM-D. With HEPES or Tris.HCl containing sodium chloride (150 mM) and/or calcium chloride (2 mM), intact vesicles adsorb on the surface until a critical density ((c)) is reached. At close vesicle contact the transformation from vesicles to supported phospholipid bilayers (SPBs) occurs. In absence of CaCl(2), the kinetics of the SPB formation process are slowed, but the passage through (c) is still observed. The latter disappears when buffers with low ionic strength were used. SPB formation was studied in a pH range of 3-10, yet the passage through (c) is obtained only for pH values above to the physiological pH (7.4-10). With an increasing vesicle concentration, (c) is reached after shorter exposure times. At a vesicle concentration of 0.01-1 mg/mL, vesicle fusion on SiO(2) proceeds with the same pathway and accelerates roughly proportionally. In contrast, the pathway of vesicle fusion is strongly influenced by the temperature in the vicinity of T(m). Above and around the T(m), transformation of vesicles to SPB proceeds smoothly, while below, a large number of nonruptured vesicles coexist with SPB. As expected, the physical state of the membrane controls the interaction with both surface and

  11. Melt Flow Control in the Directional Solidification of Binary Alloys

    NASA Technical Reports Server (NTRS)

    Zabaras, Nicholas

    2003-01-01

    Our main project objectives are to develop computational techniques based on inverse problem theory that can be used to design directional solidification processes that lead to desired temperature gradient and growth conditions at the freezing front at various levels of gravity. It is known that control of these conditions plays a significant role in the selection of the form and scale of the obtained solidification microstructures. Emphasis is given on the control of the effects of various melt flow mechanisms on the local to the solidification front conditions. The thermal boundary conditions (furnace design) as well as the magnitude and direction of an externally applied magnetic field are the main design variables. We will highlight computational design models for sharp front solidification models and briefly discuss work in progress toward the development of design techniques for multi-phase volume-averaging based solidification models.

  12. Device for passive flow control around vertical axis marine turbine

    NASA Astrophysics Data System (ADS)

    Coşoiu, C. I.; Georgescu, A. M.; Degeratu, M.; Haşegan, L.; Hlevca, D.

    2012-11-01

    The power supplied by a turbine with the rotor placed in a free stream flow may be increased by augmenting the velocity in the rotor area. The energy of the free flow is dispersed and it may be concentrated by placing a profiled structure around the bare turbine in order to concentrate more energy in the rotor zone. At the Aerodynamic and Wind Engineering Laboratory (LAIV) of the Technical University of Civil Engineering of Bucharest (UTCB) it was developed a concentrating housing to be used for hydro or aeolian horizontal axis wind turbines, in order to increase the available energy in the active section of turbine rotor. The shape of the concentrating housing results by superposing several aero/hydro dynamic effects, the most important being the one generated by the passive flow control devices that were included in the housing structure. Those concentrating housings may be also adapted for hydro or aeolian turbines with vertical axis. The present paper details the numerical research effectuated at the LAIV to determine the performances of a vertical axis marine turbine equipped with such a concentrating device, in order to increase the energy quantity extracted from the main flow. The turbine is a Darrieus type one with three vertical straight blades, symmetric with respect to the axis of rotation, generated using a NACA4518 airfoil. The global performances of the turbine equipped with the concentrating housing were compared to the same characteristics of the bare turbine. In order to validate the numerical approach used in this paper, test cases from the literature resulting from experimental and numerical simulations for similar situations, were used.

  13. Development of digital flow control system for multi-channel variable-rate sprayers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Precision modulation of nozzle flow rates is a critical step for variable-rate spray applications in orchards and ornamental nurseries. An automatic flow rate control system activated with microprocessors and pulse width modulation (PWM) controlled solenoid valves was developed to control flow rates...

  14. Flow control at low Reynolds numbers using periodic airfoil morphing

    NASA Astrophysics Data System (ADS)

    Jones, Gareth; Santer, Matthew; Papadakis, George; Bouremel, Yann; Debiasi, Marco; Imperial-NUS Joint PhD Collaboration

    2014-11-01

    The performance of airfoils operating at low Reynolds numbers is known to suffer from flow separation even at low angles of attack as a result of their boundary layers remaining laminar. The lack of mixing---a characteristic of turbulent boundary layers---leaves laminar boundary layers with insufficient energy to overcome the adverse pressure gradient that occurs in the pressure recovery region. This study looks at periodic surface morphing as an active flow control technique for airfoils in such a flight regime. It was discovered that at sufficiently high frequencies an oscillating surface is capable of not only reducing the size of the separated region---and consequently significantly reducing drag whilst simultaneously increasing lift---but it is also capable of delaying stall and as a result increasing CLmax. Furthermore, by bonding Macro Fiber Composite actuators (MFCs) to the underside of an airfoil skin and driving them with a sinusoidal frequency, it is shown that this control technique can be practically implemented in a lightweight, energy efficient way. Imperial-NUS Joint Ph.D. Programme.

  15. Dynamic flow control strategies of vehicle SCR Urea Dosing System

    NASA Astrophysics Data System (ADS)

    Lin, Wei; Zhang, Youtong; Asif, Malik

    2015-03-01

    Selective Catalyst Reduction(SCR) Urea Dosing System(UDS) directly affects the system accuracy and the dynamic response performance of a vehicle. However, the UDS dynamic response is hard to keep up with the changes of the engine's operating conditions. That will lead to low NO X conversion efficiency or NH3 slip. In order to optimize the injection accuracy and the response speed of the UDS in dynamic conditions, an advanced control strategy based on an air-assisted volumetric UDS is presented. It covers the methods of flow compensation and switching working conditions. The strategy is authenticated on an UDS and tested in different dynamic conditions. The result shows that the control strategy discussed results in higher dynamic accuracy and faster dynamic response speed of UDS. The inject deviation range is improved from being between -8% and 10% to -4% and 2% and became more stable than before, and the dynamic response time was shortened from 200 ms to 150 ms. The ETC cycle result shows that after using the new strategy the NH3 emission is reduced by 60%, and the NO X emission remains almost unchanged. The trade-off between NO X conversion efficiency and NH3 slip is mitigated. The studied flow compensation and switching working conditions can improve the dynamic performance of the UDS significantly and make the UDS dynamic response keep up with the changes of the engine's operating conditions quickly.

  16. Unsteady Aerodynamic Flow Control of a Suspended Axisymmetric Moving Platform

    NASA Astrophysics Data System (ADS)

    Lambert, Thomas; Vukasinovic, Bojan; Glezer, Ari

    2011-11-01

    The aerodynamic forces on an axisymmetric wind tunnel model are altered by fluidic interaction of an azimuthal array of integrated synthetic jet actuators with the cross flow. Four-quadrant actuators are integrated into a Coanda surface on the aft section of the body, and the jets emanate from narrow, azimuthally segmented slots equally distributed around the model's perimeter. The model is suspended in the tunnel using eight wires each comprising miniature in-line force sensors and shape-memory-alloy (SMA) strands that are used to control the instantaneous forces and moments on the model and its orientation. The interaction of the actuation jets with the flow over the moving model is investigated using PIV and time-resolved force measurements to assess the transitory aerodynamic loading effected by coupling between the induced motion of the aerodynamic surface and the fluid dynamics that is driven by the actuation. It is shown that these interactions can lead to effective control of the aerodynamic forces and moments, and thereby of the model's motion. Supported by ARO.

  17. Microprocessor-controlled pulsatile flow loop for hemodynamic studies.

    PubMed

    Lynch, T G; Hobson, R W; Pawel, H E

    1986-03-01

    Validation of spectral analytic techniques in the clinical assessment and quantitation of vascular stenoses has been aided by use of in vitro flow loops. We have used a recently developed microprocessor-controlled pulsatile flow model to examine the influence of varying stenoses on Doppler-shifted peak systolic frequencies. A nonaxisymmetric, vertically oriented stenosis was produced by extrinsic compression of latex rubber tubing 12 mm in diameter, reducing the cross-sectional area (CSA) by 25, 40, 50, 60, 70, 85, and 97%. A rolling diaphragm pump, driven through a slider-crank mechanism by a microprocessor-controlled stepper motor, generated characteristic arterial pulse waves at a rate of 75 cycles per minute. Using an 8 MHz, continuous-wave, directional Doppler velocimeter, the Doppler-shifted frequencies were recorded at the stenosis. Four sets of observations were made at each of the stenoses, and the peak systolic frequency (PSF) was determined using a spectrum analyzer. The PSF in the absence of an obstructing stenosis was 2.56 +/- 0.03 (KHz +/- SEM). This increased significantly (P less than 0.05) to 4.80 +/- 0.09 when the CSA was reduced by 50%, to 5.90 +/- 0.37 when the CSA was reduced by 60% (P less than 0.05), to 8.40 +/- 0.10 when the CSA was reduced by 70% (P less than 0.05), and to 17.84 +/- 0.89 when the CSA was reduced by 85% (P less than 0.05). These data establish the utility of this pulsatile flow model, confirming the direct relationship between the Doppler-shifted PSF and the percentage reduction in CSA.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:3951221

  18. Minnowbrook VI: 2009 Workshop on Flow Physics and Control for Internal and External Aerodynamics

    NASA Technical Reports Server (NTRS)

    LaGraff, John E.; Povinelli, Louis A.; Gostelow, J. Paul; Glauser, Mark

    2010-01-01

    Topics covered include: Flow Physics and control for Internal and External Aerodynamics (not in TOC...starts on pg13); Breaking CFD Bottlenecks in Gas-Turbine Flow-Path Design; Streamwise Vortices on the Convex Surfaces of Circular Cylinders and Turbomachinery Blading; DNS and Embedded DNS as Tools for Investigating Unsteady Heat Transfer Phenomena in Turbines; Cavitation, Flow Structure and Turbulence in the Tip Region of a Rotor Blade; Development and Application of Plasma Actuators for Active Control of High-Speed and High Reynolds Number Flows; Active Flow Control of Lifting Surface With Flap-Current Activities and Future Directions; Closed-Loop Control of Vortex Formation in Separated Flows; Global Instability on Laminar Separation Bubbles-Revisited; Very Large-Scale Motions in Smooth and Rough Wall Boundary Layers; Instability of a Supersonic Boundary-Layer With Localized Roughness; Active Control of Open Cavities; Amplitude Scaling of Active Separation Control; U.S. Air Force Research Laboratory's Need for Flow Physics and Control With Applications Involving Aero-Optics and Weapon Bay Cavities; Some Issues Related to Integrating Active Flow Control With Flight Control; Active Flow Control Strategies Using Surface Pressure Measurements; Reduction of Unsteady Forcing in a Vaned, Contra-Rotating Transonic Turbine Configuration; Active Flow Control Stator With Coanda Surface; Controlling Separation in Turbomachines; Flow Control on Low-Pressure Turbine Airfoils Using Vortex Generator Jets; Reduced Order Modeling Incompressible Flows; Study and Control of Flow Past Disk, and Circular and Rectangular Cylinders Aligned in the Flow; Periodic Forcing of a Turbulent Axisymmetric Wake; Control of Vortex Breakdown in Critical Swirl Regime Using Azimuthal Forcing; External and Turbomachinery Flow Control Working Group; Boundary Layers, Transitions and Separation; Efficiency Considerations in Low Pressure Turbines; Summary of Conference; and Final Plenary Session

  19. Lipid structure does not modify incorporation of EPA and DHA into blood lipids in healthy adults: a randomised-controlled trial.

    PubMed

    West, Annette L; Burdge, Graham C; Calder, Philip C

    2016-09-01

    Dietary supplementation is an effective means to improve EPA and DHA status. However, it is unclear whether lipid structure affects EPA+DHA bioavailability. We determined the effect of consuming different EPA and DHA lipid structures on their concentrations in blood during the postprandial period and during dietary supplementation compared with unmodified fish oil TAG (uTAG). In a postprandial cross-over study, healthy men (n 9) consumed in random order test meals containing 1·1 g EPA+0·37 g DHA as either uTAG, re-esterified TAG, free fatty acids (FFA) or ethyl esters (EE). In a parallel design supplementation study, healthy men and women (n 10/sex per supplement) consumed one supplement type for 12 weeks. Fatty acid composition was determined by GC. EPA incorporation over 6 h into TAG or phosphatidylcholine (PC) did not differ between lipid structures. EPA enrichment in NEFA was lower from EE than from uTAG (P=0·01). Plasma TAG, PC or NEFA DHA incorporation did not differ between lipid structures. Lipid structure did not affect TAG or NEFA EPA incorporation and PC or NEFA DHA incorporation following dietary supplementation. Plasma TAG peak DHA incorporation was greater (P=0·02) and time to peak shorter (P=0·02) from FFA than from uTAG in men. In both studies, the order of EPA and DHA incorporation was PC>TAG>NEFA. In conclusion, EPA and DHA lipid structure may not be an important consideration in dietary interventions. PMID:27424661

  20. Magnetoactive Sponges for Dynamic Control of Microfluidic Flow Patterns in Microphysiological Systems

    PubMed Central

    Yen, Ringo; Chan, Hon Fai; Leong, Kam W; Truskey, George A; Zhao, Xuanhe

    2014-01-01

    We developed a microfluidic flow-control system capable of dynamically generating various flow patterns on demand. The flow-control system is based on novel magnetoactive sponges embedded in microfluidic flow channels. Applying a non-uniform magnetic field compresses the magnetoactive sponge, significantly reducing porosity and hydraulic conductivity. Tuning the applied magnetic field can dynamically vary the flow rate in the microfluidic channel. Pulsatile and physiological flow patterns with frequency between 1 and 3 Hz, flow rates between 0.5 and 10 μL/min and duration over 3 weeks have been achieved. Smooth muscle cells in engineered blood vessels perfused for 7 days aligned perpendicular to the flow direction under pulsatile but not steady flow, similar to the in vivo orientation. Owing to its various advantages over traditional flow-control methods, the new system potentially has important applications in microfluidic-based microphysiological systems to simulate the physiological nature of blood flow. PMID:24310854

  1. Effects of Encapsulated Propolis on Blood Glycemic Control, Lipid Metabolism, and Insulin Resistance in Type 2 Diabetes Mellitus Rats

    PubMed Central

    Li, Yajing; Chen, Minli; Xuan, Hongzhuan; Hu, Fuliang

    2012-01-01

    The present study investigates the encapsulated propolis on blood glycemic control, lipid metabolism, and insulin resistance in type 2 diabetes mellitus (T2DM) rats. The animal characteristics and biological assays of body weight, fasting blood glucose (FBG), fasting serum insulin (FINS), insulin act index (IAI), triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured and euglycemic hyperinsulinemic glucose clamp technique were used to determine these effects. Our findings show that oral administration of encapsulated propolis can significantly inhibit the increasing of FBG and TG in T2DM rats and can improve IAI and M value in euglycemic hyperinsulinemic clamp experiment. There was no significant effects on body weight, TC, HDL-C, and LDL-C in T2DM rats treated with encapsulated propolis. In conclusion, the results indicate that encapsulated propolis can control blood glucose, modulate lipid metabolism, and improve the insulin sensitivity in T2DM rats. PMID:21716678

  2. Fecal Lipid Excretion after Consumption of a Black Tea Polyphenol Containing Beverage-Randomized, Placebo-Controlled, Double-Blind, Crossover Study.

    PubMed

    Ashigai, Hiroshi; Taniguchi, Yoshimasa; Suzuki, Mihoko; Ikeshima, Emiko; Kanaya, Tomoka; Zembutsu, Kanako; Tomita, Shimpei; Miyake, Mika; Fukuhara, Ikuo

    2016-05-01

    Obesity is a serious medical condition worldwide. Inhibition of lipid absorption is very important in preventing obesity. In a previous study, we found that postprandial elevation of triacylglycerol was suppressed by the intake of black tea polyphenol (BTP). We also reported that BTP caused lipid excretion into feces in an animal study. The present study is a clinical trial that examined lipid excretion. In this randomized, placebo-controlled, double-blind, crossover study, in the first test period participants were asked to drink either a beverage containing 55 mg BTP or a control beverage without BTP 3 times a day for 10 d. After an 11-d interval, for the second test period, they then drank the alternate test beverage 3 times a day for 10 d. During the test periods, the participants were asked to eat meals standardized according to calorie and fat content. Stool samples were obtained during the last 3 d of each test period for fecal lipid measurements. Total lipid excretion increased from 5.51±1.73 to 6.87±1.91 g/3 d after BTP intake in comparison with intake of the control beverage. These results indicated that BTP increased lipid excretion. PMID:26887502

  3. Phosphatidylinositol 4-Phosphate 5-Kinase β Controls Recruitment of Lipid Rafts into the Immunological Synapse.

    PubMed

    Kallikourdis, Marinos; Trovato, Anna Elisa; Roselli, Giuliana; Muscolini, Michela; Porciello, Nicla; Tuosto, Loretta; Viola, Antonella

    2016-02-15

    Phosphatidylinositol 4,5-biphosphate (PIP2) is critical for T lymphocyte activation serving as a substrate for the generation of second messengers and the remodeling of actin cytoskeleton necessary for the clustering of lipid rafts, TCR, and costimulatory receptors toward the T:APC interface. Spatiotemporal analysis of PIP2 synthesis in T lymphocytes suggested that distinct isoforms of the main PIP2-generating enzyme, phosphatidylinositol 4-phosphate 5-kinase (PIP5K), play a differential role on the basis of their distinct localization. In this study, we analyze the contribution of PIP5Kβ to T cell activation and show that CD28 induces the recruitment of PIP5Kβ to the immunological synapse, where it regulates filamin A and lipid raft accumulation, as well as T cell activation, in a nonredundant manner. Finally, we found that Vav1 and the C-terminal 83 aa of PIP5Kβ are pivotal for the PIP5Kβ regulatory functions in response to CD28 stimulation. PMID:26773155

  4. Regulation of lipid synthesis by the RNA helicase Mov10 controls Wnt5a production

    PubMed Central

    Wang, W; Snyder, N; Worth, A J; Blair, I A; Witze, E S

    2015-01-01

    Expression of the Wnt ligand Wnt5a is frequently elevated in melanoma and is thought to be a critical regulator of cell movement during metastasis. However, the mechanisms regulating its expression are unknown. We find that the level of secreted Wnt5a varies by as much as 10-fold between cell lines and correlates more strongly with invasion than total cellular levels. Our results indicate that the RNA helicase Mov10 plays a role in Wnt5a synthesis and secretion. Inhibition of Mov10 increases secreted Wnt5a levels in melanoma cells by increasing Wnt5a synthesis and acylation. This is achieved by increasing fatty acid synthase (FASN) and stearoyl-CoA desaturase expression, leading to elevated levels of palmitoleoyl-CoA, required for Wnt ligand lipid modification and secretion. Melanoma tumors exhibit reduced expression of Mov10 compared with benign nevi and Mov10 levels inversely correlate with FASN levels in primary tumors. These results reveal a previously unappreciated role for aberrant lipid metabolism in regulating Wnt5a signaling that may be a critical step in melanoma progression. PMID:26029828

  5. Fluorinated amphiphiles control the insertion of α-hemolysin pores into lipid bilayers.

    PubMed

    Raychaudhuri, Pinky; Li, Qiuhong; Mason, Amy; Mikhailova, Ellina; Heron, Andrew J; Bayley, Hagan

    2011-03-15

    The insertion of fully folded and assembled ion channels and pores into planar lipid bilayers for electrical recording has been facilitated by the use of conventional detergents at a final concentration below the critical micelle concentration (CMC). After the desired number of channels or pores (often one) has been incorporated into a bilayer, it is important to prevent further insertion events, which is often done by awkward techniques such as perfusion. Here, we show that the addition of single-chain fluorinated amphiphiles (F-amphiphiles) with zwitterionic, simple neutral, and neutral oligomeric headgroups at a concentration above the CMC prevents the further insertion of staphylococcal α-hemolysin pores, MspA pores, and Kcv potassium channels into lipid bilayers. We found the commercially available F(6)FC (fluorinated fos-choline with a C(6)F(13)C(2)H(4) chain) to be the least perturbing and most effective agent for this purpose. Bilayers are known to be resistant to F-amphiphiles, which in this case we suppose sequester the pores and channels within amphiphile aggregates. We suggest that F-amphiphiles might be useful in the fabrication of bilayer arrays for nanopore sensor devices and the rapid screening of membrane proteins. PMID:21275394

  6. Backbone of complex networks of corporations: the flow of control.

    PubMed

    Glattfelder, J B; Battiston, S

    2009-09-01

    We present a methodology to extract the backbone of complex networks based on the weight and direction of links, as well as on nontopological properties of nodes. We show how the methodology can be applied in general to networks in which mass or energy is flowing along the links. In particular, the procedure enables us to address important questions in economics, namely, how control and wealth are structured and concentrated across national markets. We report on the first cross-country investigation of ownership networks, focusing on the stock markets of 48 countries around the world. On the one hand, our analysis confirms results expected on the basis of the literature on corporate control, namely, that in Anglo-Saxon countries control tends to be dispersed among numerous shareholders. On the other hand, it also reveals that in the same countries, control is found to be highly concentrated at the global level, namely, lying in the hands of very few important shareholders. Interestingly, the exact opposite is observed for European countries. These results have previously not been reported as they are not observable without the kind of network analysis developed here. PMID:19905177

  7. Backbone of complex networks of corporations: The flow of control

    NASA Astrophysics Data System (ADS)

    Glattfelder, J. B.; Battiston, S.

    2009-09-01

    We present a methodology to extract the backbone of complex networks based on the weight and direction of links, as well as on nontopological properties of nodes. We show how the methodology can be applied in general to networks in which mass or energy is flowing along the links. In particular, the procedure enables us to address important questions in economics, namely, how control and wealth are structured and concentrated across national markets. We report on the first cross-country investigation of ownership networks, focusing on the stock markets of 48 countries around the world. On the one hand, our analysis confirms results expected on the basis of the literature on corporate control, namely, that in Anglo-Saxon countries control tends to be dispersed among numerous shareholders. On the other hand, it also reveals that in the same countries, control is found to be highly concentrated at the global level, namely, lying in the hands of very few important shareholders. Interestingly, the exact opposite is observed for European countries. These results have previously not been reported as they are not observable without the kind of network analysis developed here.

  8. Lipid profile and glucose changes after supplementation with astaxanthin: a systematic review and meta-analysis of randomized controlled trials

    PubMed Central

    Ursoniu, Sorin; Sahebkar, Amirhossein; Banach, Maciej

    2015-01-01

    Introduction Many studies have shown that oral supplementation with astaxanthin may be a novel potential treatment for inflammation and oxidative stress in cardiovascular diseases, but evidence of the effects on lipid profile and glucose is still inconclusive. Therefore, we performed a meta-analysis to evaluate the efficacy of astaxanthin supplementation on plasma lipid and glucose concentrations. Material and methods The search included PubMed, Cochrane Library, Scopus, and EMBASE (up to November 27, 2014) to identify randomized controlled trials (RCTs) investigating the effects of astaxanthin supplementation on lipid profile and glucose levels. Two independent reviewers extracted data on study characteristics, methods and outcomes. Results Seven studies meeting inclusion criteria with 280 participants were selected for this meta-analysis; 163 participants were allocated to the astaxanthin supplementation group and 117 to the control group. A random-effect meta-analysis of data from 7 RCTs (10 treatment arms) did not show any significant effect of supplementation with astaxanthin on plasma concentrations of total cholesterol (weighted mean difference (WMD): –1.52 mg/dl, 95% CI: –8.69 to –5.66, p = 0.679), LDL-C (WMD: +1.25 mg/dl, 95% CI: –6.70 to +9.21, p = 0.758), HDL-C (WMD: +1.75 mg/dl, 95% CI: –0.92 to +4.42, p = 0.199), triglycerides (WMD: –4.76 mg/dl, 95% CI: –21.52 to +12.00, p = 0.578), or glucose (WMD: –2.65 mg/dl, 95% CI: –5.84 to +0.54, p = 0.103). All these effect sizes were robust, and omission of any of the included studies did not significantly change the overall estimate. Conclusions This meta-analysis of data from 10 RCT arms did not indicate a significant effect of supplementation with astaxanthin on plasma lipid profile, but a slight glucose-lowering effect was observed. Further, well-designed trials are necessary to validate these results. PMID:25995739

  9. Dielectric barrier discharge plasma actuator for flow control

    NASA Astrophysics Data System (ADS)

    Opaits, Dmitry Florievich

    Electrohydrodynamic (EHD) and magnetohydrodynamic phenomena are being widely studied for aerodynamic applications. The major effects of these phenomena are heating of the gas, body force generation, and enthalpy addition or extraction, [1, 2, 3]. In particular, asymmetric dielectric barrier discharge (DBD) plasma actuators are known to be effective EHD device in aerodynamic control, [4, 5]. Experiments have demonstrated their effectiveness in separation control, acoustic noise reduction, and other aeronautic applications. In contrast to conventional DBD actuators driven by sinusoidal voltages, we proposed and used a voltage profile consisting of nanosecond pulses superimposed on dc bias voltage. This produces what is essentially a non-self-sustained discharge: the plasma is generated by repetitive short pulses, and the pushing of the gas occurs primarily due to the bias voltage. The advantage of this non-self-sustained discharge is that the parameters of ionizing pulses and the driving bias voltage can be varied independently, which adds flexibility to control and optimization of the actuators performance. Experimental studies were conducted of a flow induced in a quiescent room air by a single DBD actuator. A new approach for non-intrusive diagnostics of plasma actuator induced flows in quiescent gas was proposed, consisting of three elements coupled together: the Schlieren technique, burst mode of plasma actuator operation, and 2-D numerical fluid modeling. During the experiments, it was found that DBD performance is severely limited by surface charge accumulation on the dielectric. Several ways to mitigate the surface charge were found: using a reversing DC bias potential, three-electrode configuration, slightly conductive dielectrics, and semi conductive coatings. Force balance measurements proved the effectiveness of the suggested configurations and advantages of the new voltage profile (pulses+bias) over the traditional sinusoidal one at relatively low

  10. Advanced Flow Control as a Management Tool in the National Airspace System

    NASA Technical Reports Server (NTRS)

    Wugalter, S.

    1974-01-01

    Advanced Flow Control is closely related to Air Traffic Control. Air Traffic Control is the business of the Federal Aviation Administration. To formulate an understanding of advanced flow control and its use as a management tool in the National Airspace System, it becomes necessary to speak somewhat of air traffic control, the role of FAA, and their relationship to advanced flow control. Also, this should dispell forever, any notion that advanced flow control is the inspirational master valve scheme to be used on the Alaskan Oil Pipeline.

  11. Minimal sensor count approach to fuzzy logic rotary blood pump flow control.

    PubMed

    Casas, Fernando; Ahmed, Nisar; Reeves, Andrew

    2007-01-01

    A rotary blood pump fuzzy logic flow controller without flow sensors was developed and tested in vitro. The controller, implemented in LabView, was set to maintain a flow set point in the presence of external pressure disturbances. Flow was estimated as a function of measured pump's delta P and speed, using a steady-state, nonlinear approximation. The fuzzy controller used the pump's flow estimate and delta P as feedback variables. The defuzzified control output manipulated the pump speed. Membership functions included flow error, delta P, and pump speed. Experimental runs in a mock loop (water/glycerin 3.5 cPs, 37 degrees C), using the estimated flow, were compared with those using a Transonic flow meter for nine conditions of flow and delta P (4 to 6 L/min, 150 to 350 mm Hg). Pressure disturbances generated by a servo pinch valve ranged from +/-23 to +/-47 mm Hg. Results indicated that the fuzzy controller ably regulated the flow set point to within +/-10% of the baseline even under large swings in pressure. There was no difference in controller performance between the ultrasonic flow measurement and the estimated flow calculation scenarios. These tests demonstrated that the fuzzy controller is capable of rejecting disturbances and regulating flow to acceptable limits while using a flow estimate. PMID:17413551

  12. Protein functionalized nanomaterials for flow control, biocatalysis and architectural organization

    NASA Astrophysics Data System (ADS)

    Nednoor, Pramod

    This dissertation work describes the construction of biomolecule-functionalized nanomaterials for applications in ion channel mimics, biocatalysis and supramolecular architectures. The core entrances of an aligned carbon nanotube membrane were functionalized with a desthiobiotin derivative that binds reversibly to streptavidin, thereby enabling a reversible closing/opening of the core entrance. Ionic flux through the CNT membrane was monitored using optically absorbing charged marker molecules. The flux was reduced by a factor of 24 when the desthiobiotin on the CNT was coordinated with streptavidin; release of streptavidin increased the flux, demonstrating a reversible ion-channel flow. Analysis of solutions of released streptavidin showed approximately 16 bound streptavidin molecules per CNT tip. Following on similar lines, a nine residue synthetic peptide containing a serine residue [G-R-T-G-R-R-N-S-I-NH2], which is a specific substrate of Protein Kinase A was functionalized at the tip of carbon nanotubes to obtain a biomimetic system where phosphorylation regulates ligand-gated ion channels. Phosphorylation of the serine residue with a kinase led to the binding of a monoclonal anti-phosphoserine antibody. This binding event controlled the ionic flow through the pores. Dephosphorylating the serine residue with an alkaline phosphatase prevented the antibody from binding, thereby altering the flow through the channels. The transport of oppositely charged molecules through the CNT membrane was quantified. Nanoscale materials (i.e., nanoparticles and nanorods) are an attractive platform for applications in biotransformations and biosensors. Conjugation of a fullerene derivative to a mutant subtilisin was demonstrated, and the effect of the fullerene on the enzyme activity was determined. The fullerene-conjugated enzyme had improved catalytic properties in comparison to subtilisin immobilized on nonporous silica. Further, the pH profile of free and fullerene

  13. Automated gas control with the Maquet FLOW-i.

    PubMed

    Carette, Rik; De Wolf, Andre M; Hendrickx, Jan F A

    2016-06-01

    The FLOW-i anesthesia machine (Maquet, Solna, Sweden) can be equipped with automated gas control (AGC), an automated low flow tool with target control of the inspired oxygen concentration (FIO2) and end-expired concentration (FA) of a potent inhaled anesthetic. We examined the performance and quantitative aspects of the AGC. After IRB approval and individual informed consent, anesthesia in 24 ASA I-II patients undergoing abdominal or gynecological surgery was maintained with sevoflurane in O2/air with a target FIO2 of 40 % and a target sevoflurane FA (FAsevo) of 2.0 %. The AGC tool also allows the user to select 1 out of 9 different speeds with which the target FAsevo can be reached (with 9 being the fastest speed). Eight patients each were randomly assigned to speed 2, 4, and 6 (= group 2, group 4, and group 6, respectively); these three speeds were chosen arbitrarily. AGC was activated immediately after securing the airway, which defined the start of the study, and the study ended 60 min later. The following parameters were compared among the three groups: age, height, weight, FIO2, FAsevo, BIS values, heart rate, mean arterial blood pressure, fresh gas flow, and sevoflurane usage. Agent usage as reported by the FLOW-i was compared among the three groups. Patient demographics and maintenance FGF did not differ among groups. A very short-lived very high FGF (≈20 L min(-1) for 8-12 s) ensured that the target FIO2 was attained within 1-2 min in all patients. FAsevo was 1.8 % after 15, 10, and 6 min, and 1.9 % after 30, 20 and 15 min in groups 2, 4, and 6, respectively. Blood pressure, heart rate, and BIS values did not differ among the three groups. BIS values remained acceptable in all patients, even with the slowest speed. Cumulative agent usage differed among all three groups between 2 and 30 min (lower with the lower speed), and between group 2 and 6 between 35 and 60 min. AGC combines an exponentially decreasing FGF pattern with a choice of ramp

  14. The Role of Neuronal Signaling in Controlling Cerebral Blood Flow

    ERIC Educational Resources Information Center

    Drake, Carrie T.; Iadecola, Costantino

    2007-01-01

    Well-regulated blood flow within the brain is vital to normal function. The brain's requirement for sufficient blood flow is ensured by a tight link between neural activity and blood flow. The link between regional synaptic activity and regional cerebral blood flow, termed functional hyperemia, is the basis for several modern imaging techniques…

  15. Chaos control for the plates subjected to subsonic flow

    NASA Astrophysics Data System (ADS)

    Norouzi, Hamed; Younesian, Davood

    2016-07-01

    The suppression of chaotic motion in viscoelastic plates driven by external subsonic air flow is studied. Nonlinear oscillation of the plate is modeled by the von-Kármán plate theory. The fluid-solid interaction is taken into account. Galerkin's approach is employed to transform the partial differential equations of the system into the time domain. The corresponding homoclinic orbits of the unperturbed Hamiltonian system are obtained. In order to study the chaotic behavior of the plate, Melnikov's integral is analytically applied and the threshold of the excitation amplitude and frequency for the occurrence of chaos is presented. It is found that adding a parametric perturbation to the system in terms of an excitation with the same frequency of the external force can lead to eliminate chaos. Variations of the Lyapunov exponent and bifurcation diagrams are provided to analyze the chaotic and periodic responses. Two perturbation-based control strategies are proposed. In the first scenario, the amplitude of control forces reads a constant value that should be precisely determined. In the second strategy, this amplitude can be proportional to the deflection of the plate. The performance of each controller is investigated and it is found that the second scenario would be more efficient.

  16. Flow-induced vibration of a steam control valve

    NASA Astrophysics Data System (ADS)

    Yonezawa, Koichi; Ogawa, Ryohei; Ogi, Kanako; Takino, Tomofumi; Tsujimoto, Yoshinobu; Endo, Takahide; Tezuka, Kenichi; Morita, Ryo; Inada, Fumio

    2012-11-01

    Main steam control valves in power plants are required to operate underwide ranges of valve openings and pressure ratios. In the present paper, experimental and numerical investigations are conducted using rigid and flexible valve head supports to clarify the mechanisms of valve head vibrations that are caused by unsteady flows around the valve. The results obtained using the rigid support without valve head vibration show that the unsteady flow around the valve head causes pressure fluctuations on the valve head surface with random and impulsive wave forms. When using the flexible support, the valve head vibrates near the natural frequency of the valve head support system, and vibrations are excited around the operating conditions where the pressure fluctuation becomes greater when using the rigidly supported valve head. When the valve head vibration increases, the pressure fluctuation becomes periodic with the same frequency as the valve head vibration. The numerical results show that the response of the separated jet lags behind the valve head motion. As a result, the lateral fluid force adds negative damping on the vibration on the valve head.

  17. Computational optimization of a pneumatic forebody flow control concept

    NASA Technical Reports Server (NTRS)

    Gee, Ken; Tavella, Domingo; Schiff, Lewis B.

    1991-01-01

    The effectiveness of a tangential slot blowing concept for generating lateral control forces on an aircraft forebody is analyzed using computational fluid dynamics. The flow about a fighter forebody is computed using a multiple-zone, thin-layer Navier-Stokes code. Tangential slot blowing is modeled by the use of an actuator plane. The effects of slot location and slot length on the efficiency of the system are analyzed. Results of the study indicate that placement of the slot near the nose of the aircraft greatly enhances the efficiency of the system, while the length and circumferential location of the slot are of secondary importance. Efficiency is defined by the amount of side force or yawing moment obtained per unit blowing coefficient. The effect of sideslip on the system is also analyzed. The system is able to generate incremental changes in forces and moments in flows with sideslip angles up to 10 deg comparable to those obtained at zero sideslip. These results are used to determine a baseline configuration for an experimental study of the tangential slot blowing concept.

  18. Predictive onboard flow control for packet switching satellites

    NASA Technical Reports Server (NTRS)

    Bobinsky, Eric A.

    1992-01-01

    We outline two alternate approaches to predicting the onset of congestion in a packet switching satellite, and argue that predictive, rather than reactive, flow control is necessary for the efficient operation of such a system. The first method discussed is based on standard, statistical techniques which are used to periodically calculate a probability of near-term congestion based on arrival rate statistics. If this probability exceeds a present threshold, the satellite would transmit a rate-reduction signal to all active ground stations. The second method discussed would utilize a neural network to periodically predict the occurrence of buffer overflow based on input data which would include, in addition to arrival rates, the distributions of packet lengths, source addresses, and destination addresses.

  19. GASP cloud encounter statistics - Implications for laminar flow control flight

    NASA Technical Reports Server (NTRS)

    Jasperson, W. H.; Nastrom, G. D.; Davis, R. E.; Holdeman, J. D.

    1984-01-01

    The cloud observation archive from the NASA Global Atmospheric Sampling Program (GASP) is analyzed in order to derive the probability of cloud encounter at altitudes normally flown by commercial airliners, for application to a determination of the feasability of Laminar Flow Control (LFC) on long-range routes. The probability of cloud encounter is found to vary significantly with season. Several meteorological circulation features are apparent in the latitudinal distribution of cloud cover. The cloud encounter data are shown to be consistent with the classical midlatitude cyclone model with more clouds encountered in highs than in lows. Aircraft measurements of route-averaged time-in-clouds fit a gamma probability distribution model which is applied to estimate the probability of extended cloud encounter, and the associated loss of LFC effectiveness along seven high-density routes. The probability is demonstrated to be low.

  20. Computational investigation of slot blowing for fuselage forebody flow control

    NASA Technical Reports Server (NTRS)

    Murman, Scott M.; Rizk, Yehia M.; Schiff, Lewis B.; Cummings, Russell M.

    1992-01-01

    This paper presents a computational investigation of a tangential slot blowing concept for generating lateral control forces on an aircraft fuselage forebody. The effects of varying both the jet width and jet exit velocity for a fixed location slot are analyzed. This work is aimed at aiding researchers in designing future experimental and computational models of tangential slot blowing. The primary influence on the resulting side force of the forebody is seen to be the jet mass flow rate. This influence is sensitive to different combinations of slot widths and jet velocities over the range of variables considered. Both an actuator plane and an overset grid technique are used to model the tangential slot. The overset method successfully resolves the details of the actual slot geometry, extending the generality of the numerical method. The actuator plane concept predicts side forces similar to those produced by resolving the actual slot geometry.

  1. Natural laminar flow and airplane stability and control

    NASA Technical Reports Server (NTRS)

    Vandam, Cornelis P.

    1986-01-01

    Location and mode of transition from laminar to turbulent boundary layer flow have a dominant effect on the aerodynamic characteristics of an airfoil section. The influences of these parameters on the sectional lift and drag characteristics of three airfoils are examined. Both analytical and experimental results demonstrate that when the boundary layer transitions near the leading edge as a result of surface roughness, extensive trailing-edge separation of the turbulent boundary layer may occur. If the airfoil has a relatively sharp leading-edge, leading-edge stall due to laminar separation can occur after the leading-edge suction peak is formed. These two-dimensional results are used to examine the effects of boundary layer transition behavior on airplane longitudinal and lateral-directional stability and control.

  2. Study and Control of Scour below Pipelines under unidirectional flow

    NASA Astrophysics Data System (ADS)

    Kabiri, Shima; Hoseinzadeh Dalir, Ali

    2016-04-01

    Water and other fluids pipelines laid on sandy rivers and sea bed change flow pattern around pipelines. These changes increase the bed shear stress and the degree of confusion around the pipes and cause to create scour hole below the pipes. In this situation, the occurrence of scour below the pipelines may lead to instability, fracture and bending and even breakage where cause very severe economic and environmental harms eventually. In this research as well as studying of scour under the pipelines, the bed sill had been used as a new mechanism in order to reduce and control of scour. For this purpose, 3 pipes (smooth) with different diameters (D) were modelled in flow condition of PIC U/Uc=0.8-0.9 in the channel with 11m length, 25cm width and depth of 50 cm. Experiment has been performed in below 2 modes: 1) Scour below a smooth pipe without bed sill 2) Scour below a smooth pipe with bed sill. In the 2nd modes bed sill was located at 4 different distances (L=0,D/4,D/2,D) of downstream Of the pipe central axis. In the experiments bed sill was a barrier for spreading wake vortices and it controlled erosions of downstream. Results of this research indicated that whatever the distance of bed sill from central axis of pipe is less, there is the most influence in reducing the scour depth below pipe. In the case that bed sill had been located exactly under central axis of pipe, scour depth under pipe decreased about 100% Also in this situation with passing a long time from the beginning of examination, the pipe self-burial process occurred due to vortex creation in pipe downstream and relocation of particles toward pipe.

  3. The Efficacy of Boswellia Serrata Gum Resin for Control of Lipid Profile and Blood Glucose in Diabetic Patients

    PubMed Central

    Mehrzadi, Saeed; Tavakolifar, Bahreh; Huseini, Hasan Fallah; Mosavat, Seyed Hamdollah; Heydari, Mojtaba

    2016-01-01

    Background: Regarding preclinical evidence for antidiabetic effects of Boswellia serrata, we evaluated anti-hyperglycemic and lipid-lowering effects of Boswellia serrate gum resin in type 2 diabetic patients in a double-blind randomized placebo-control trial. Methods: Fifty-six diabetic patients were randomly allocated to two groups to receive 250 mg of the Boswellia serrate gum resin or placebo twice daily for 8 weeks, in addition to their routine antidiabetic treatments. Fasting blood sugar (FBS), glycosylated hemoglobin (HbA1c), insulin level, total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglyceride of serum were measured before and after the intervention. Results: Although there was a considerable reduction after the intervention in FBS (P=0.04), HbA1c (P=0.02) and triglyceride (P=0.01) in the Boswellia serrate gum resin group, no significant difference was observed in all outcome measures between the two groups at the end of the study (FBS P=0.09, HbA1c P=0.20, total cholesterol P=0.31, LDL P=0.49, HDL P=0.10, triglyceride P=0.78 and insulin level P=0.86). Conclusion: The current study showed the 8 weeks complementary use of Boswellia serrate gum resin with a daily dose of 500 mg had no better glucose and lipid lowering effect than placebo in diabetic patients. PMID:27516696

  4. Phosphatidylserine stimulation of Drs2p·Cdc50p lipid translocase dephosphorylation is controlled by phosphatidylinositol-4-phosphate.

    PubMed

    Jacquot, Aurore; Montigny, Cédric; Hennrich, Hanka; Barry, Raphaëlle; le Maire, Marc; Jaxel, Christine; Holthuis, Joost; Champeil, Philippe; Lenoir, Guillaume

    2012-04-13

    Here, Drs2p, a yeast lipid translocase that belongs to the family of P(4)-type ATPases, was overexpressed in the yeast Saccharomyces cerevisiae together with Cdc50p, its glycosylated partner, as a result of the design of a novel co-expression vector. The resulting high yield allowed us, using crude membranes or detergent-solubilized membranes, to measure the formation from [γ-(32)P]ATP of a (32)P-labeled transient phosphoenzyme at the catalytic site of Drs2p. Formation of this phosphoenzyme could be detected only if Cdc50p was co-expressed with Drs2p but was not dependent on full glycosylation of Cdc50p. It was inhibited by orthovanadate and fluoride compounds. In crude membranes, the phosphoenzyme formed at steady state at 4 °C displayed ADP-insensitive but temperature-sensitive decay. Solubilizing concentrations of dodecyl maltoside left this decay rate almost unaltered, whereas several other detergents accelerated it. Unexpectedly, the dephosphorylation rate for the solubilized Drs2p·Cdc50p complex was inhibited by the addition of phosphatidylserine. Phosphatidylserine exerted its anticipated accelerating effect on the dephosphorylation of Drs2p·Cdc50p complex only in the additional presence of phosphatidylinositol-4-phosphate. These results explain why phosphatidylinositol-4-phosphate tightly controls Drs2p-catalyzed lipid transport and establish the functional relevance of the Drs2p·Cdc50p complex overexpressed here. PMID:22351780

  5. Mismatch discrimination of lipidated DNA and LNA-probes (LiNAs) in hybridization-controlled liposome assembly.

    PubMed

    Jakobsen, Ulla; Vogel, Stefan

    2016-08-01

    Assays for mismatch discrimination and detection of single nucleotide variations by hybridization-controlled assembly of liposomes, which do not require tedious surface chemistry, are versatile for both DNA and RNA targets. We report herein a comprehensive study on different DNA and LNA (locked nucleic acids) probe designs, including membrane-anchoring requirements, studies on different probes and target lengths (including overhangs), DNA and RNA targets (including sequences associated with pathogens) for lipidated nucleic acids (LiNAs). Advantages and limitations of the liposome assembly based assay in the context of mismatch discrimination and SNP detection are presented. The advantages of membrane-anchored LiNA-probes compared to chemically attached probes on solid nanoparticles (e.g. gold nanoparticles) are described. Key functionalities such as non-covalent attachment of LiNA probes without the need for long spacers and the inherent mobility of membrane-anchored probes in lipid-bilayer membranes will be described for several different probe designs. PMID:27356098

  6. Stable carbon isotopic compositions of intact polar lipids reveal complex carbon flow patterns among hydrocarbon degrading microbial communities at the Chapopote asphalt volcano

    NASA Astrophysics Data System (ADS)

    Schubotz, Florence; Lipp, Julius S.; Elvert, Marcus; Hinrichs, Kai-Uwe

    2011-08-01

    Seepage of asphalt forms the basis of a cold seep system at 3000 m water depth at the Chapopote Knoll in the southern Gulf of Mexico. Anaerobic microbial communities are stimulated in the oil-impregnated sediments as evidenced by the presence of intact polar membrane lipids (IPLs) derived from archaea and Bacteria at depths up to 7 m below the seafloor. Detailed investigation of stable carbon isotope composition (δ 13C) of alkyl and acyl moieties derived from a range of IPL precursors with distinct polar head groups resolved the complexity of carbon metabolisms and utilization of diverse carbon sources by uncultured microbial communities. In surface sediments most of the polar lipid-derived fatty acids with phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) head groups could be tentatively assigned to autotrophic sulfate-reducing bacteria, with a relatively small proportion involved in the anaerobic oxidation of methane. Derivatives of phosphatidyl-( N)-methylethanolamine (PME) were abundant and could be predominantly assigned to heterotrophic oil-degrading bacteria. Archaeal IPLs with phosphate-based hydroxyarchaeols and diglycosidic glyceroldibiphytanylglyceroltetraethers (GDGTs) were assigned to methanotrophic archaea of the ANME-2 and ANME-1 cluster, respectively, whereas δ 13C values of phosphate-based archaeols and mixed phosphate-based and diglycosidic GDGTs point to methanogenic archaea. At a 7 m deep sulfate-methane transition zone that is linked to the upward movement of gas-laden petroleum, a distinct increase in abundance of archaeal IPLs such as phosphate-based hydroxyarchaeols and diglycosidic archaeol and GDGTs is observed; their δ 13C values are consistent with their origin from both methanotrophic and methanogenic archaea. This study reveals previously hidden, highly complex patterns in the carbon-flow of versatile microbial communities involved in the degradation of heavy oil including hydrocarbon gases

  7. Flow Diode and Method for Controlling Fluid Flow Origin of the Invention

    NASA Technical Reports Server (NTRS)

    Dyson, Rodger W (Inventor)

    2015-01-01

    A flow diode configured to permit fluid flow in a first direction while preventing fluid flow in a second direction opposite the first direction is disclosed. The flow diode prevents fluid flow without use of mechanical closures or moving parts. The flow diode utilizes a bypass flowline whereby all fluid flow in the second direction moves into the bypass flowline having a plurality of tortuous portions providing high fluidic resistance. The portions decrease in diameter such that debris in the fluid is trapped. As fluid only travels in one direction through the portions, the debris remains trapped in the portions.

  8. Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana.

    PubMed

    Li, Tingting; Gargouri, Mahmoud; Feng, Jie; Park, Jeong-Jin; Gao, Difeng; Miao, Chao; Dong, Tao; Gang, David R; Chen, Shulin

    2015-03-01

    Microalgae have attracted growing attention due to their potential in biofuel feedstock production. However, current understanding of the regulatory mechanisms for lipid biosynthesis and storage in microalgae is still limited. This study revealed that the microalga Chlorella sorokiniana showed sequential accumulation of starch and lipids. When nitrogen was replete and/or depleted over a short period, starch was the predominant carbon storage form with basal levels of lipid accumulation. After prolonged nitrogen depletion, lipid accumulation increased considerably, which was partially due to starch degradation, as well as the turnover of primary metabolites. Lipid accumulation is also strongly dependent on the linear electron flow of photosynthesis, peaking at lower light intensities. Collectively, this study reveals a relatively clear regulation pattern of starch and lipid accumulation that is basically controlled by nitrogen levels. The mixotrophic growth of C. sorokiniana shows promise for biofuel production in terms of lipid accumulation in the final biomass. PMID:25616239

  9. Boosting target tracking using particle filter with flow control

    NASA Astrophysics Data System (ADS)

    Moshtagh, Nima; Chan, Moses W.

    2013-05-01

    Target detection and tracking with passive infrared (IR) sensors can be challenging due to significant degradation and corruption of target signature by atmospheric transmission and clutter effects. This paper summarizes our efforts in phenomenology modeling of boosting targets with IR sensors, and developing algorithms for tracking targets in the presence of background clutter. On the phenomenology modeling side, the clutter images are generated using a high fidelity end-to-end simulation testbed. It models atmospheric transmission, structured clutter and solar reflections to create realistic background images. The dynamics and intensity of a boosting target are modeled and injected onto the background scene. Pixel level images are then generated with respect to the sensor characteristics. On the tracking analysis side, a particle filter for tracking targets in a sequence of clutter images is developed. The particle filter is augmented with a mechanism to control particle flow. Specifically, velocity feedback is used to constrain and control the particles. The performance of the developed "adaptive" particle filter is verified with tracking of a boosting target in the presence of clutter and occlusion.

  10. Unveiling astrocytic control of cerebral blood flow with optogenetics

    PubMed Central

    Masamoto, Kazuto; Unekawa, Miyuki; Watanabe, Tatsushi; Toriumi, Haruki; Takuwa, Hiroyuki; Kawaguchi, Hiroshi; Kanno, Iwao; Matsui, Ko; Tanaka, Kenji F.; Tomita, Yutaka; Suzuki, Norihiro

    2015-01-01

    Cortical neural activities lead to changes in the cerebral blood flow (CBF), which involves astrocytic control of cerebrovascular tone. However, the manner in which astrocytic activity specifically leads to vasodilation or vasoconstriction is difficult to determine. Here, cortical astrocytes genetically expressing a light-sensitive cation channel, channelrhodopsin-2 (ChR2), were transcranially activated with a blue laser while the spatiotemporal changes in CBF were noninvasively monitored with laser speckle flowgraphy in the anesthetised mouse cortex. A brief photostimulation induced a fast transient increase in CBF. The average response onset time was 0.7 ± 0.7 sec at the activation foci, and this CBF increase spread widely from the irradiation spot with an apparent propagation speed of 0.8–1.1 mm/sec. The broad increase in the CBF could be due to a propagation of diffusible vasoactive signals derived from the stimulated astrocytes. Pharmacological manipulation showed that topical administration of a K+ channel inhibitor (BaCl2; 0.1–0.5 mM) significantly reduced the photostimulation-induced CBF responses, which indicates that the ChR2-evoked astrocytic activity involves K+ signalling to the vascular smooth muscle cells. These findings demonstrate a unique model for exploring the role of the astrocytes in gliovascular coupling using non-invasive, time-controlled, cell-type specific perturbations. PMID:26076820

  11. Suppression of vortex-induced vibration of a circular cylinder using suction-based flow control

    NASA Astrophysics Data System (ADS)

    Chen, Wen-Li; Xin, Da-Bo; Xu, Feng; Li, Hui; Ou, Jin-Ping; Hu, Hui

    2013-10-01

    In the present study, a flow control method is employed to mitigate vortex-induced vibration (VIV) of a circular cylinder by using a suction flow method. The VIV of a circular cylinder was first reproduced in a wind tunnel by using a spring-mass system. The time evolution of the cylinder oscillation and the time histograms of the surface pressures of 119 taps in four sections of the circular cylinder model were measured during the wind tunnel experiments. Four steady suction flow rates were used to investigate the effectiveness of the suction control method to suppress VIV of the circular cylinder. The vibration responses, the mean and fluctuating pressure coefficients, and the resultant aerodynamic force coefficients of the circular cylinder under the suction flow control are analyzed. The measurement results indicate clearly that the steady suction flow control method exhibits excellent control effectiveness and can distinctly suppress the VIV by dramatically reducing the amplitudes of cylinder vibrations, fluctuating pressure coefficients and lift coefficients of the circular cylinder model. By comparing the test cases with different suction flow rates, it is found that there exists an optimal suction flow rate for the maximum VIV control. The cases with higher suction flow rates do not necessarily behave better than those with lower suction flow rates. With the experimental setting used in the present study, the suction flow control method is found to behave better for VIV suppression when the ratio of the suction flow velocity to the oncoming flow velocity is less than one.

  12. Topics in Transcriptional Control of Lipid Metabolism: from Transcription Factors to Gene-Promoter Polymorphisms

    PubMed Central

    Bergen, Werner G.; Burnett, Derris D.

    2013-01-01

    The central dogma of biology (DNA>>RNA>>Protein) has remained as an extremely useful scaffold to guide the study of molecular regulation of cellular metabolism. Molecular regulation of cellular metabolism has been pursued from an individual enzyme to a global assessment of protein function at the genomic (DNA), transcriptomic (RNA) and translation (Protein) levels. Details of a key role by inhibitory small RNAs and post-translational processing of cellular proteins on a whole cell/global basis are now just emerging. Below we emphasize the role of transcription factors (TF) in regulation of adipogenesis and lipogenesis. Additionally we have also focused on emerging additional TF that may also have hitherto unrecognized roles in adipogenesis and lipogenesis as compared to our present understanding. It is generally recognized that SNPs in structural genes can affect the final structure/function of a given protein. The implications of SNPs located in the non-transcribed promoter region on transcription have not been examined as extensively at this time. Here we have also summarized some emerging results on promoter SNPs for lipid metabolism and related cellular processes. PMID:25031651

  13. Viral infection controlled by a calcium-dependent lipid-binding module in ALIX.

    PubMed

    Bissig, Christin; Lenoir, Marc; Velluz, Marie-Claire; Kufareva, Irina; Abagyan, Ruben; Overduin, Michael; Gruenberg, Jean

    2013-05-28

    ALIX plays a role in nucleocapsid release during viral infection, as does lysobisphosphatidic acid (LBPA). However, the mechanism remains unclear. Here we report that LBPA is recognized within an exposed site in ALIX Bro1 domain predicted by MODA, an algorithm for discovering membrane-docking areas in proteins. LBPA interactions revealed a strict requirement for a structural calcium tightly bound near the lipid interaction site. Unlike other calcium- and phospholipid-binding proteins, the all-helical triangle-shaped fold of the Bro1 domain confers selectivity for LBPA via a pair of hydrophobic residues in a flexible loop, which undergoes a conformational change upon membrane association. Both LBPA and calcium binding are necessary for endosome association and virus infection, as are ALIX ESCRT binding and dimerization capacity. We conclude that LBPA recruits ALIX onto late endosomes via the calcium-bound Bro1 domain, triggering a conformational change in ALIX to mediate the delivery of viral nucleocapsids to the cytosol during infection. PMID:23664863

  14. The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.

    PubMed

    Han, Jinbo; Li, Erwei; Chen, Liqun; Zhang, Yuanyuan; Wei, Fangchao; Liu, Jieyuan; Deng, Haiteng; Wang, Yiguo

    2015-08-13

    Abnormal accumulation of triglycerides in the liver, caused in part by increased de novo lipogenesis, results in non-alcoholic fatty liver disease and insulin resistance. Sterol regulatory element-binding protein 1 (SREBP1), an important transcriptional regulator of lipogenesis, is synthesized as an inactive precursor that binds to the endoplasmic reticulum (ER). In response to insulin signalling, SREBP1 is transported from the ER to the Golgi in a COPII-dependent manner, processed by proteases in the Golgi, and then shuttled to the nucleus to induce lipogenic gene expression; however, the mechanisms underlying enhanced SREBP1 activity in insulin-resistant obesity and diabetes remain unclear. Here we show in mice that CREB regulated transcription coactivator 2 (CRTC2) functions as a mediator of mTOR signalling to modulate COPII-dependent SREBP1 processing. CRTC2 competes with Sec23A, a subunit of the COPII complex, to interact with Sec31A, another COPII subunit, thus disrupting SREBP1 transport. During feeding, mTOR phosphorylates CRTC2 and attenuates its inhibitory effect on COPII-dependent SREBP1 maturation. As hepatic overexpression of an mTOR-defective CRTC2 mutant in obese mice improved the lipogenic program and insulin sensitivity, these results demonstrate how the transcriptional coactivator CRTC2 regulates mTOR-mediated lipid homeostasis in the fed state and in obesity. PMID:26147081

  15. VARIABLE FIRING RATE OIL BURNER USING PULSE FUEL FLOW CONTROL.

    SciTech Connect

    KRISHNA,C.R.; BUTCHER,T.A.; KAMATH,B.R.

    2004-10-01

    The residential oil burner market is currently dominated by the pressure-atomized retention head burner, which has an excellent reputation for reliability and efficiency. In this burner, oil is delivered to a fuel nozzle at pressures from 100 to 150 psi. In addition, to atomizing the fuel, the small, carefully controlled size of the nozzle exit orifice serves to control the burner firing rate. Burners of this type are currently available at firing rates of more than 0.5 gallons-per-hour (70,000 Btu/hr). Nozzles have been made for lower firing rates, but experience has shown that such nozzles suffer rapid fouling of the necessarily small passages, leading to bad spray patterns and poor combustion performance. Also, traditionally burners and the nozzles are oversized to exceed the maximum demand. Typically, this is figured as follows. The heating load of the house on the coldest day for the location is considered to define the maximum heat load. The contractor or installer adds to this to provide a safety margin and for future expansion of the house. If the unit is a boiler that provides domestic hot water through the use of a tankless heating coil, the burner capacity is further increased. On the contrary, for a majority of the time, the heating system is satisfying a much smaller load, as only rarely do all these demands add up. Consequently, the average output of the heating system has to be much less than the design capacity and this is accomplished by start and stop cycling operation of the system so that the time-averaged output equals the demand. However, this has been demonstrated to lead to overall efficiencies lower than the steady-state efficiency. Therefore, the two main reasons for the current practice of using oil burners much larger than necessary for space heating are the unavailability of reliable low firing rate oil burners and the desire to assure adequate input rate for short duration, high draw domestic hot water loads. One approach to solve this

  16. Selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast Rhodosporidium toruloides NCYC 921 using flow cytometry.

    PubMed

    Freitas, Cláudia; Parreira, Teresa Margarida; Roseiro, José; Reis, Alberto; da Silva, Teresa Lopes

    2014-04-01

    The present work studied low-cost carbon sources for carotenoid and lipid production using the yeast Rhodosporidum toruloides NCYC 921. Carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in R. toruloides batch cultivations. Carob pulp syrup containing a total sugar concentration of 75 g L(-1) induced the highest total fatty acid productivity (1.90 g L(-1)h(-1)) and the highest carotenoid productivity (9.79 μg L(-1)h(-1)). Flow cytometric analysis revealed that most of the yeast cells (>60%) grown on carob pulp syrup displayed intact polarised membranes, conversely to the cells grown on sugarcane molasses, wherein a large proportion (>45%) displayed permeabilised cytoplasmic membranes. PMID:24650616

  17. Interaction Between Strategic and Local Traffic Flow Controls

    NASA Technical Reports Server (NTRS)

    Grabbe, Son; Sridhar, Banavar; Mukherjee, Avijit; Morando, Alexander

    2010-01-01

    The loosely coordinated sets of traffic flow management initiatives that are operationally implemented at the national- and local-levels have the potential to under, over, and inconsistently control flights. This study is designed to explore these interactions through fast-time simulations with an emphasis on identifying inequitable situations in which flights receive multiple uncoordinated delays. Two operationally derived scenarios were considered in which flights arriving into the Dallas/Fort Worth International Airport were first controlled at the national-level, either with a Ground Delay Program or a playbook reroute. These flights were subsequently controlled at the local level. The Traffic Management Advisor assigned them arrival scheduling delays. For the Ground Delay Program scenarios, between 51% and 53% of all arrivals experience both pre-departure delays from the Ground Delay Program and arrival scheduling delays from the Traffic Management Advisor. Of the subset of flights that received multiple delays, between 5.7% and 6.4% of the internal departures were first assigned a pre-departure delay by the Ground Delay Program, followed by a second pre-departure delay as a result of the arrival scheduling. For the playbook reroute scenario, Dallas/Fort Worth International Airport arrivals were first assigned pre-departure reroutes based on the MW_2_DALLAS playbook plan, and were subsequently assigned arrival scheduling delays by the Traffic Management Advisor. Since the airport was operating well below capacity when the playbook reroute was in effect, only 7% of the arrivals were observed to receive both rerouting and arrival scheduling delays. Findings from these initial experiments confirm field observations that Ground Delay Programs operated in conjunction with arrival scheduling can result in inequitable situations in which flights receive multiple uncoordinated delays.

  18. Englacial Structures as Indicators of the Controls on Ice Flow

    NASA Astrophysics Data System (ADS)

    Holschuh, N.; Parizek, B. R.; Alley, R. B.; Anandakrishnan, S.

    2015-12-01

    Direct sampling of the subglacial environment is costly, and will therefore never supply the spatial coverage needed to determine the basal boundary conditions required for large-scale ice-sheet modeling. Studies of the West Antarctic Ice Sheet (WAIS) show that the frictional and rheologic properties of the bed are a leading control on the evolution of the system, so developing geophysical methods to help constrain the basal characteristics of WAIS will reduce uncertainty in predictions of the timing and magnitude of future sea-level rise. Radar-imaged structures within the ice are an attractive data set for this pursuit, as they contain information about the flow dynamics that transform the horizontally deposited layers to their modern configuration; however, they can be challenging to interpret, given the number of processes acting to deform the internal layers and the difficulty in automating their analysis. In this study, we move away from the layer-tracing paradigm in favor of an automated slope extraction algorithm. This has several advantages: it does not require feature-continuity, providing a more stable result in regions of intense deformation, and it results in a data product that maps directly to model output. For steady-state features, layer slopes reflect the horizontal and vertical velocity structure, making quantitative comparison of the model and observations simple compared to the more qualitative, particle tracer comparisons done in the past. Using a higher order ice-flow model, we attempt to refine our understanding of basal properties using reflector slope fields at the grounding line of Whillans Ice Stream and the shear margin of the North East Greenland Ice Stream, with the hope of eventually using this method for basin-scale inversions.

  19. CFD-based aero-optical analysis of flow fields over two-dimensional cavities with active flow control

    NASA Astrophysics Data System (ADS)

    Tan, Yan

    Prediction and control of optical wave front distortions and aberrations in a high energy laser beam due to interaction with an unsteady highly non-uniform flow field is of great importance in the development of directed energy weapon systems for Unmanned Air Vehicles (UAV). The unsteady shear layer over the weapons bay cavity is the primary cause of this distortion of the optical wave front. The large scale vortical structure of the shear layer over the cavity can be significantly reduced by employing an active flow control technique combined with passive flow control. This dissertation explores various active and passive control methods to suppress the cavity oscillations and thereby improve the aero-optics of cavity flow. In active flow control technique, a steady or a pulsed jet is applied at the sharp leading edge of cavities of different aspect ratios L/D (=2, 4, 15), where L and D are the width and the depth of a cavity respectively. In the passive flow control approach, the sharp leading or trailing edge of the cavity is modified into a round edge of different radii. Both of these active and passive flow control approaches are studied independently and in combination. Numerical simulations are performed, with and without active flow control for subsonic free stream flow past two-dimensional sharp and round leading or trailing edge cavities using Unsteady Reynolds-Averaged Navier-Stokes (URANS) equations with a two-equation Shear Stress Transport (SST) turbulence model or a hybrid SST/Large Eddy Simulation (LES) model. Aero-optical analysis is developed and applied to all the simulation cases. Index of refraction and Optical Path Difference (OPD) are compared for flow fields without and with active flow control. Root-Mean-Square (RMS) value of OPD is calculated and compared with the experimental data, where available. The effect of steady and pulsed blowing on buffet loading on the downstream face of the cavity is also computed. Using the numerical

  20. The Role of Storage Lipids in the Relation between Fecundity, Locomotor Activity, and Lifespan of Drosophila melanogaster Longevity-Selected and Control Lines

    PubMed Central

    Nasiri Moghadam, Neda; Holmstrup, Martin; Manenti, Tommaso; Brandt Mouridsen, Marie; Pertoldi, Cino; Loeschcke, Volker

    2015-01-01

    The contribution of insect fat body to multiple processes, such as development, metamorphosis, activity, and reproduction results in trade-offs between life history traits. In the present study, age-induced modulation of storage lipid composition in Drosophila melanogaster longevity-selected (L) and non-selected control (C) lines was studied and the correlation between total body fat mass and lifespan assessed. The trade-offs between fecundity, locomotor activity, and lifespan were re-evaluated from a lipid-related metabolic perspective. Fewer storage lipids in the L lines compared to the C lines supports the impact of body fat mass on extended lifespan. The higher rate of fecundity and locomotor activity in the L lines may increase the lipid metabolism and enhance the lipolysis of storage lipids, reducing fat reserves. The correlation between neutral lipid fatty acids and fecundity, as well as locomotor activity, varied across age groups and between the L and C lines. The fatty acids that correlated with egg production were different from the fatty acids that correlated with locomotor activity. The present study suggests that fecundity and locomotor activity may positively affect the lifespan of D. melanogaster through the inhibition of fat accumulation. PMID:26115349