Science.gov

Sample records for conventional mismatch controls

  1. Dynamic control of strand excision during human DNA mismatch repair

    PubMed Central

    Jeon, Yongmoon; Kim, Daehyung; Martín-López, Juana V.; Lee, Ryanggeun; Oh, Jungsic; Hanne, Jeungphill; Fishel, Richard; Lee, Jong-Bong

    2016-01-01

    Mismatch repair (MMR) is activated by evolutionarily conserved MutS homologs (MSH) and MutL homologs (MLH/PMS). MSH recognizes mismatched nucleotides and form extremely stable sliding clamps that may be bound by MLH/PMS to ultimately authorize strand-specific excision starting at a distant 3′- or 5′-DNA scission. The mechanical processes associated with a complete MMR reaction remain enigmatic. The purified human (Homo sapien or Hs) 5′-MMR excision reaction requires the HsMSH2–HsMSH6 heterodimer, the 5′ → 3′ exonuclease HsEXOI, and the single-stranded binding heterotrimer HsRPA. The HsMLH1–HsPMS2 heterodimer substantially influences 5′-MMR excision in cell extracts but is not required in the purified system. Using real-time single-molecule imaging, we show that HsRPA or Escherichia coli EcSSB restricts HsEXOI excision activity on nicked or gapped DNA. HsMSH2–HsMSH6 activates HsEXOI by overcoming HsRPA/EcSSB inhibition and exploits multiple dynamic sliding clamps to increase tract length. Conversely, HsMLH1–HsPMS2 regulates tract length by controlling the number of excision complexes, providing a link to 5′ MMR. PMID:26951673

  2. Decentralized Adaptive Control of Systems with Uncertain Interconnections, Plant-Model Mismatch and Actuator Failures

    NASA Technical Reports Server (NTRS)

    Patre, Parag; Joshi, Suresh M.

    2011-01-01

    Decentralized adaptive control is considered for systems consisting of multiple interconnected subsystems. It is assumed that each subsystem s parameters are uncertain and the interconnection parameters are not known. In addition, mismatch can exist between each subsystem and its reference model. A strictly decentralized adaptive control scheme is developed, wherein each subsystem has access only to its own state but has the knowledge of all reference model states. The mismatch is estimated online for each subsystem and the mismatch estimates are used to adaptively modify the corresponding reference models. The adaptive control scheme is extended to the case with actuator failures in addition to mismatch.

  3. A Direct Adaptive Control Approach in the Presence of Model Mismatch

    NASA Technical Reports Server (NTRS)

    Joshi, Suresh M.; Tao, Gang; Khong, Thuan

    2009-01-01

    This paper considers the problem of direct model reference adaptive control when the plant-model matching conditions are violated due to abnormal changes in the plant or incorrect knowledge of the plant's mathematical structure. The approach consists of direct adaptation of state feedback gains for state tracking, and simultaneous estimation of the plant-model mismatch. Because of the mismatch, the plant can no longer track the state of the original reference model, but may be able to track a new reference model that still provides satisfactory performance. The reference model is updated if the estimated plant-model mismatch exceeds a bound that is determined via robust stability and/or performance criteria. The resulting controller is a hybrid direct-indirect adaptive controller that offers asymptotic state tracking in the presence of plant-model mismatch as well as parameter deviations.

  4. Post-transplantation cyclophosphamide versus conventional graft-versus-host disease prophylaxis in mismatched unrelated donor haematopoietic cell transplantation.

    PubMed

    Mehta, Rohtesh S; Saliba, Rima M; Chen, Julianne; Rondon, Gabriela; Hammerstrom, Aimee E; Alousi, Amin; Qazilbash, Muzaffar; Bashir, Qaiser; Ahmed, Sairah; Popat, Uday; Hosing, Chitra; Khouri, Issa; Shpall, Elizabeth J; Champlin, Richard E; Ciurea, Stefan O

    2016-05-01

    Post-transplantation cyclophosphamide (PTCy) is an effective strategy to prevent graft-versus-host disease (GVHD) after haploidentical haematopoietic cell transplantation (HCT). We determined the efficacy of PTCy-based GVHD prophylaxis in human leucocyte antigen (HLA)-mismatched unrelated donor (MMUD) HCT. We analysed 113 adult patients with high-risk haematological malignancies who underwent one-antigen MMUD transplantation between 2009 and 2013. Of these, 41 patients received PTCy, tacrolimus and mycophenolate mofetil (MMF) for GVHD prophylaxis; 72 patients received conventional prophylaxis with anti-thymocyte globulin, tacrolimus and methotrexate. Graft source was primarily bone marrow (83% PTCy vs. 63% conventional group). Incidence of grade II-IV (37% vs. 36%, P = 0·8) and grade III-IV (17% vs. 12%, P = 0·5) acute GVHD was similar at day 100. However, the incidence of grade II-IV acute GVHD by day 30 was significantly lower in the PTCy group (0% vs. 15%, P = 0·01). Median time to neutrophil (18 days vs. 12 days, P < 0·001) and platelet (25·5 days vs. 18 days, P = 0·05) engraftment was prolonged in PTCy group. Rates of graft failure, chronic GVHD, 2-year non-relapse mortality, relapse, progression-free survival or overall survival were similar. Our results demonstrate that PTCy, tacrolimus and MMF for GVHD prophylaxis is safe and produced similar results as conventional prophylaxis in patients with one antigen HLA-MMUD HCT. PMID:26947769

  5. XTOD to Conventional Facilities Interface Control Document

    SciTech Connect

    McMahon, D

    2005-09-29

    This document describes the interface between the LCLS X-ray Transport and Diagnostics (XTOD) (WBS 1.5) and the LCLS Conventional Facilities (CF) (WBS 1.1). The interface locations ranging from the beam dump to the far experimental hall are identified. Conventional Facilities provides x-ray, beamline and equipment enclosures, mounting surfaces, conventional utilities, compressed (clean, dry) air, process and purge gases, exhaust systems, power, and environmental conditions for the XTOD components and controls.

  6. Chattering free adaptive multivariable sliding mode controller for systems with matched and mismatched uncertainty.

    PubMed

    Mondal, Sanjoy; Mahanta, Chitralekha

    2013-05-01

    In this paper, a chattering free adaptive sliding mode controller (SMC) is proposed for stabilizing a class of multi-input multi-output (MIMO) systems affected by both matched and mismatched types of uncertainties. The proposed controller uses a proportional plus integral sliding surface whose gain is adaptively tuned to prevent overestimation. A vertical take-off and landing (VTOL) aircraft system is simulated to demonstrate the effectiveness of the proposed control scheme. PMID:23357555

  7. Mismatch Negativity (MMN) in Freely-Moving Rats with Several Experimental Controls

    PubMed Central

    Harms, Lauren; Fulham, W. Ross; Todd, Juanita; Budd, Timothy W.; Hunter, Michael; Meehan, Crystal; Penttonen, Markku; Schall, Ulrich; Zavitsanou, Katerina; Hodgson, Deborah M.; Michie, Patricia T.

    2014-01-01

    Mismatch negativity (MMN) is a scalp-recorded electrical potential that occurs in humans in response to an auditory stimulus that defies previously established patterns of regularity. MMN amplitude is reduced in people with schizophrenia. In this study, we aimed to develop a robust and replicable rat model of MMN, as a platform for a more thorough understanding of the neurobiology underlying MMN. One of the major concerns for animal models of MMN is whether the rodent brain is capable of producing a human-like MMN, which is not a consequence of neural adaptation to repetitive stimuli. We therefore tested several methods that have been used to control for adaptation and differential exogenous responses to stimuli within the oddball paradigm. Epidural electroencephalographic electrodes were surgically implanted over different cortical locations in adult rats. Encephalographic data were recorded using wireless telemetry while the freely-moving rats were presented with auditory oddball stimuli to assess mismatch responses. Three control sequences were utilized: the flip-flop control was used to control for differential responses to the physical characteristics of standards and deviants; the many standards control was used to control for differential adaptation, as was the cascade control. Both adaptation and adaptation-independent deviance detection were observed for high frequency (pitch), but not low frequency deviants. In addition, the many standards control method was found to be the optimal method for observing both adaptation effects and adaptation-independent mismatch responses in rats. Inconclusive results arose from the cascade control design as it is not yet clear whether rats can encode the complex pattern present in the control sequence. These data contribute to a growing body of evidence supporting the hypothesis that rat brain is indeed capable of exhibiting human-like MMN, and that the rat model is a viable platform for the further investigation of the

  8. Controllable Mismatched Ligation for Bioluminescence Screening of Known and Unknown Mutations.

    PubMed

    Xu, Qinfeng; Huang, Si-Qiang; Ma, Fei; Tang, Bo; Zhang, Chun-Yang

    2016-02-16

    Single-nucleotide polymorphisms (SNPs) are closely related to human diseases and individual drug responses, and the accurate detection of SNPs is crucial to both clinical diagnosis and development of personalized medicine. Among various SNPs detection methods, ligase detection reaction (LDR) has shown great potential due to its low detection limit and excellent specificity. However, frequent involvement of expensive labels increases the experimental cost and compromises the assay efficiency, and the requirement of careful predesigned probes limits it to only known SNPs assays. In this research, we develop a controllable mismatched ligation for bioluminescence screening of both known and unknown mutations. Especially, the ligation specificity of E. coli ligase is tunable under different experimental conditions. The mismatches locating on the 3'-side of the nick cannot be ligated efficiently by E. coli ligase, whereas all mismatches locating on the 5'-side of the nick can be ligated efficiently by E. coli ligase. We design a 3'-discriminating probe (3'-probe) for the discrimination of known mutation and introduce a T7 Endo I for the detection of unknown mutation. With the integration of bioluminescence monitoring of ligation byproduct adenosine 5'-monophosphate (AMP), both known and unknown SNPs can be easily detected without the involvement of any expensive labels and labor-intensive separation. This method is simple, homogeneous, label-free, and cost-effective and may provide a valuable complement to current sequencing technologies for disease diagnostics, personalized medicine, and biomedical research. PMID:26754165

  9. Robust control of nonlinear MAGLEV suspension system with mismatched uncertainties via DOBC approach.

    PubMed

    Yang, Jun; Zolotas, Argyrios; Chen, Wen-Hua; Michail, Konstantinos; Li, Shihua

    2011-07-01

    Robust control of a class of uncertain systems that have disturbances and uncertainties not satisfying "matching" condition is investigated in this paper via a disturbance observer based control (DOBC) approach. In the context of this paper, "matched" disturbances/uncertainties stand for the disturbances/uncertainties entering the system through the same channels as control inputs. By properly designing a disturbance compensation gain, a novel composite controller is proposed to counteract the "mismatched" lumped disturbances from the output channels. The proposed method significantly extends the applicability of the DOBC methods. Rigorous stability analysis of the closed-loop system with the proposed method is established under mild assumptions. The proposed method is applied to a nonlinear MAGnetic LEViation (MAGLEV) suspension system. Simulation shows that compared to the widely used integral control method, the proposed method provides significantly improved disturbance rejection and robustness against load variation. PMID:21349514

  10. Developing controllable hypermutable Clostridium cells through manipulating its methyl-directed mismatch repair system.

    PubMed

    Luan, Guodong; Cai, Zhen; Gong, Fuyu; Dong, Hongjun; Lin, Zhao; Zhang, Yanping; Li, Yin

    2013-11-01

    Development of controllable hypermutable cells can greatly benefit understanding and harnessing microbial evolution. However, there have not been any similar systems developed for Clostridium, an important bacterial genus. Here we report a novel two-step strategy for developing controllable hypermutable cells of Clostridium acetobutylicum, an important and representative industrial strain. Firstly, the mutS/L operon essential for methyldirected mismatch repair (MMR) activity was inactivated from the genome of C. acetobutylicum to generate hypermutable cells with over 250-fold increased mutation rates. Secondly, a proofreading control system carrying an inducibly expressed mutS/L operon was constructed. The hypermutable cells and the proofreading control system were integrated to form a controllable hypermutable system SMBMutC, of which the mutation rates can be regulated by the concentration of anhydrotetracycline (aTc). Duplication of the miniPthl-tetR module of the proofreading control system further significantly expanded the regulatory space of the mutation rates, demonstrating hypermutable Clostridium cells with controllable mutation rates are generated. The developed C. acetobutylicum strain SMBMutC2 showed higher survival capacities than the control strain facing butanol-stress, indicating greatly increased evolvability and adaptability of the controllable hypermutable cells under environmental challenges. PMID:24214875

  11. Transcription of eucaryotic tRNA1met and 5SRNA genes by RNA polymerase III is blocked by base mismatches in the intragenic control regions.

    PubMed Central

    Sullivan, M A; Folk, W R

    1987-01-01

    We have constructed duplex DNAs containing single G-T or A-C mismatches in the X. laevis tRNA1met gene. Mismatches within regions of this gene which are bound by transcription factor TFIIIC prevent transcription by RNA polymerase III. Homoduplexes with G-C----A-T mutations at some of the same sites, however, are transcribed efficiently in oocytes. Mismatches outside of the tRNA1met gene have no effect upon transcription. A survey of several point mutants in the Syrian hamster 5SRNA gene indicates that mismatches outside the internal control region somewhat reduce transcription, but a mismatch within the internal control region blocks transcription. Thus, the presence of mismatched bases in the region of DNA which interacts with RNA polymerase III transcription factors blocks transcription, perhaps by interfering with DNA renaturation following transit of the RNA polymerase. Images PMID:3645544

  12. Antimony-mediated control of misfit dislocations and strain at the highly lattice mismatched GaSb/GaAs interface.

    PubMed

    Wang, Yi; Ruterana, Pierre; Chen, Jun; Kret, Slawomir; El Kazzi, Salim; Genevois, Cecile; Desplanque, Ludovic; Wallart, Xavier

    2013-10-01

    Determining the atomic structure of misfit dislocations at highly lattice mismatched interface is essential to optimize the quality of the epitaxial layer. Here, with aberration corrected scanning transmission electron microscopy at sub-Angstrom resolution and molecular dynamics simulation, we investigated the atomic structure of misfit dislocations at GaSb/GaAs interface. New types of Lomer misfit dislocation formed on an Sb wetting monolayer were observed, in contrast to a conventional misfit dislocation whose core is located at interface. These Sb-mediated dislocations have highly localized cores and offer more capability to confine the mismatch strain at the interface. The low strain atomic configuration of Sb-mediated dislocations is driven by minimization of the core energy. This unveiled mechanism may pave the way to the growth of high quality hetero-epitaxial layers. PMID:24024581

  13. Artificial mismatch hybridization

    DOEpatents

    Guo, Zhen; Smith, Lloyd M.

    1998-01-01

    An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.

  14. Unconventional digital reactor control without conventional programming

    SciTech Connect

    Edwards, R.M.; Johns, R.M.; Kenney, S.J.

    1995-12-31

    Recent advances in simulation technology have resulted in the capability to design, test, and implement advanced control algorithms without the need for the labor-intensive effort of writing and debugging of computer programs. This technology has been adopted for a program of experimental development of power reactor control, which is jointly sponsored by the National Science Foundation and the Electric Power Research Institute. The experimental reactor control test bed utilizes the General Atomic Mark III TRIGA reactor at the Penn State Breazeale reactor facility. Control experiments are conducted within the movable experiment technical specifications of the TRIGA. A digital controller with an experimental control algorithm is interfaced to a secondary control rod (SCR). The new technology presented in this paper utilizes a UNIX network-compatible microprocessor-based controller operating under the Wind River Systems VxWorks real-time operating system. The controller interfaces with the Math-works MATLAB/SIMULINK development environment and Real-Time Innovations 8 monitoring software remotely operated on a SPARC workstation.

  15. Adaptive Neural Stabilizing Controller for a Class of Mismatched Uncertain Nonlinear Systems by State and Output Feedback.

    PubMed

    Arefi, Mohammad Mehdi; Jahed-Motlagh, Mohammad Reza; Karimi, Hamid Reza

    2015-08-01

    In this paper, first, an adaptive neural network (NN) state-feedback controller for a class of nonlinear systems with mismatched uncertainties is proposed. By using a radial basis function NN (RBFNN), a bound of unknown nonlinear functions is approximated so that no information about the upper bound of mismatched uncertainties is required. Then, an observer-based adaptive controller based on RBFNN is designed to stabilize uncertain nonlinear systems with immeasurable states. The state-feedback and observer-based controllers are based on Lyapunov and strictly positive real-Lyapunov stability theory, respectively, and it is shown that the asymptotic convergence of the closed-loop system to zero is achieved while maintaining bounded states at the same time. The presented methods are more general than the previous approaches, handling systems with no restriction on the dimension of the system and the number of inputs. Simulation results confirm the effectiveness of the proposed methods in the stabilization of mismatched nonlinear systems. PMID:25265641

  16. Energy losses of nanomechanical resonators induced by atomic force microscopy-controlled mechanical impedance mismatching

    PubMed Central

    Rieger, Johannes; Isacsson, Andreas; Seitner, Maximilian J.; Kotthaus, Jörg P.; Weig, Eva M.

    2014-01-01

    Clamping losses are a widely discussed damping mechanism in nanoelectromechanical systems, limiting the performance of these devices. Here we present a method to investigate this dissipation channel. Using an atomic force microscope tip as a local perturbation in the clamping region of a nanoelectromechanical resonator, we increase the energy loss of its flexural modes by at least one order of magnitude. We explain this by a transfer of vibrational energy into the cantilever, which is theoretically described by a reduced mechanical impedance mismatch between the resonator and its environment. A theoretical model for this mismatch, in conjunction with finite element simulations of the evanescent strain field of the mechanical modes in the clamping region, allows us to quantitatively analyse data on position and force dependence of the tip-induced damping. Our experiments yield insights into the damping of nanoelectromechanical systems with the prospect of engineering the energy exchange in resonator networks. PMID:24594876

  17. Origins of the WHO Framework Convention on Tobacco Control

    PubMed Central

    Roemer, Ruth; Taylor, Allyn; Lariviere, Jean

    2005-01-01

    The World Health Organization (WHO) Framework Convention on Tobacco Control originated in 1993 with a decision by Ruth Roemer and Allyn Taylor to apply to tobacco control Taylor’s idea that the WHO should utilize its constitutional authority to develop international conventions to advance global health. In 1995, Taylor and Ruth Roemer proposed various options to WHO, recommending the framework convention-protocol approach conceptualized by Taylor. Despite initial resistance by some WHO officials, this approach gained wide acceptance. In 1996, the World Health Assembly voted to proceed with its development. Negotiations by WHO member states led the World Health Assembly in May 2003 to adopt by consensus the WHO Framework Convention on Tobacco Control—the first international treaty adopted under WHO auspices. The treaty formally entered into force for state parties on February 27, 2005. PMID:15914812

  18. Conflict and metacognitive control: the mismatch-monitoring hypothesis of how others' knowledge states affect recall.

    PubMed

    Fraundorf, Scott H; Benjamin, Aaron S

    2016-09-01

    Information about others' success in remembering is frequently available. For example, students taking an exam may assess its difficulty by monitoring when others turn in their exams. In two experiments, we investigated how rememberers use this information to guide recall. Participants studied paired associates, some semantically related (and thus easier to retrieve) and some unrelated (and thus harder). During a subsequent cued recall test, participants viewed fictive information about an opponent's accuracy on each item. In Experiment 1, participants responded to each cue once before seeing the opponent's performance and once afterwards. Participants reconsidered their responses least often when the opponent's accuracy matched the item difficulty (easy items the opponent recalled, hard items the opponent forgot) and most often when the opponent's accuracy and the item difficulty mismatched. When participants responded only after seeing the opponent's performance (Experiment 2), the same mismatch conditions that led to reconsideration even produced superior recall. These results suggest that rememberers monitor whether others' knowledge states accord or conflict with their own experience, and that this information shifts how they interrogate their memory and what they recall. PMID:26247369

  19. Interfacial Octahedral Rotation Mismatch Control of the Symmetry and Properties of SrRuO3.

    PubMed

    Gao, Ran; Dong, Yongqi; Xu, Han; Zhou, Hua; Yuan, Yakun; Gopalan, Venkatraman; Gao, Chen; Fong, Dillon D; Chen, Zuhuang; Luo, Zhenlin; Martin, Lane W

    2016-06-15

    Epitaxial strain can be used to tune the properties of complex oxides with perovskite structure. Beyond just lattice mismatch, the use of octahedral rotation mismatch at heterointerfaces could also provide an effective route to manipulate material properties. Here, we examine the evolution of the structural motif (i.e., lattice parameters, symmetry, and octahedral rotations) of SrRuO3 films grown on substrates engineered to have the same lattice parameters, but different octahedral rotations. SrRuO3 films grown on SrTiO3 (001) (no octahedral rotations) and GdScO3-buffered SrTiO3 (001) (with octahedral rotations) substrates are found to exhibit monoclinic and tetragonal symmetry, respectively. Electrical transport and magnetic measurements reveal that the tetragonal films exhibit higher resistivity, lower magnetic Curie temperatures, and more isotropic magnetism as compared to those with monoclinic structure. Synchrotron-based quantification of the octahedral rotation network reveals that the tilting pattern in both film variants is the same (albeit with slightly different magnitudes of in-plane rotation angles). The abnormal rotation pattern observed in tetragonal SrRuO3 indicates a possible decoupling between the internal octahedral rotation and lattice symmetry, which could provide new opportunities to engineer thin-film structure and properties. PMID:27219026

  20. Somatostatin Interneurons Control a Key Component of Mismatch Negativity in Mouse Visual Cortex.

    PubMed

    Hamm, Jordan P; Yuste, Rafael

    2016-07-19

    Patients with schizophrenia have deficient sensory processing, undermining how they perceive and relate to a changing environment. This impairment can be captured by the reduced mismatch negativity (MMN) index, an electroencephalographic biomarker of psychosis. The biological factors contributing to MMN are unclear, though mouse research, in which genetic and optical methods could be applied, has given some insight. Using fast two-photon calcium imaging and multielectrode recordings in awake mice, we find that visual cortical circuits display adapted (decreased) responses to repeated stimuli and amplified responses to a deviant stimulus, the key component of human MMN. Moreover, pharmacogenetic silencing of somatostatin-containing interneurons specifically eliminated this amplification, along with its associated theta/alpha-band response, leaving stimulus-specific adaption and related gamma-band modulations intact. Our results validate a mouse model of MMN and suggest that abnormalities in somatostatin-containing interneurons cause sensory deficits underlying MMN and schizophrenia. PMID:27396334

  1. CONTROL OF CRYPTOSPORIDIUM OOCYSTS BY STEADY-STATE CONVENTIONAL TREATMENT

    EPA Science Inventory

    Pilot-scale experiments have been performed to assess the ability of conventional treatment to control Cryptosporidium oocysts under steady-state conditions. The work was performed with a pilot plant that was designed to minimize flow rates and, as a result, the number of oocyst...

  2. Mismatch discrimination of lipidated DNA and LNA-probes (LiNAs) in hybridization-controlled liposome assembly.

    PubMed

    Jakobsen, Ulla; Vogel, Stefan

    2016-08-01

    Assays for mismatch discrimination and detection of single nucleotide variations by hybridization-controlled assembly of liposomes, which do not require tedious surface chemistry, are versatile for both DNA and RNA targets. We report herein a comprehensive study on different DNA and LNA (locked nucleic acids) probe designs, including membrane-anchoring requirements, studies on different probes and target lengths (including overhangs), DNA and RNA targets (including sequences associated with pathogens) for lipidated nucleic acids (LiNAs). Advantages and limitations of the liposome assembly based assay in the context of mismatch discrimination and SNP detection are presented. The advantages of membrane-anchored LiNA-probes compared to chemically attached probes on solid nanoparticles (e.g. gold nanoparticles) are described. Key functionalities such as non-covalent attachment of LiNA probes without the need for long spacers and the inherent mobility of membrane-anchored probes in lipid-bilayer membranes will be described for several different probe designs. PMID:27356098

  3. Distributing flow mismatches in supply-constrained irrigation canals through feedback control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The operation of main irrigation canals is complicated in situations where the operator does not have full control over the canal inflow, or where there are very long transmission distances from the point of supply, or both. Experienced operators are able to control the canal, but often supply error...

  4. Monitoring commercial conventional facilities control with the APS control system: The Metasys-to-EPICS interface

    SciTech Connect

    Nawrocki, G.J.; Seaver, C.L.; Kowalkowski, J.B.

    1995-12-31

    As controls needs at the Advanced Photon Source matured from an installation phase to an operational phase, the need to monitor the existing conventional facilities control system with the EPICS-based accelerator control system was realized. This existing conventional facilities control network is based on a proprietary system from Johnson Controls called Metasys. Initially read-only monitoring of the Metasys parameters will be provided; however, the ability for possible future expansion to full control is available. This paper describes a method of using commercially available hardware and existing EPICS software as a bridge between the Metasys and EPICS control systems.

  5. Conformational arm-wrestling: battles for stereochemical control in benzamides bearing matched and mismatched chiral 2- and 6-substituents.

    PubMed

    Clayden, Jonathan; Foricher, Yann J Y; Helliwell, Madeleine; Johnson, Paul; Mitjans, David; Vinader, Victoria

    2006-02-01

    The orientation of a tertiary amide group adjacent to an aromatic ring may be governed by the stereochemistry of an adjacent chiral substituent. With a chiral substituent in both ortho positions, matched/mismatched pairs of isomers result. Evidence for matched stereochemistry is provided by the clean NMR spectra of single conformers, while mismatching gives poor or unexpected selectivities in the formation of chiral substituents, or mixtures of amide conformers. Attempts to use the match-mismatch effect to select for racemic pairs of enantiomeric substituents, and hence develop a "racemate-sequestering" reagent, are described, along with the use of "matching" to scavenge a single enantiomer of a diamine from material of incomplete enantiomeric purity. PMID:16446801

  6. Metals in combined conventional and vegetated road runoff control systems.

    PubMed

    Pontier, H; Williams, J B; May, E

    2001-01-01

    Combined conventional and vegetated runoff treatment systems were installed on the rural Newbury Bypass in Southern England, which was estimated to carry an average daily traffic of 30,000 vehicles. The system components were arranged in series to give progressive pollutant removal, comprising an oil separator, sediment trap, grassy slope and constructed wetland. In the absence of specific design criteria for road runoff control, the system design was based on available guidance notes. Since the road opened, an 18-month monitoring programme has examined the performance of these systems. Early indications are that the systems, and especially the wetlands, promote removal of suspended solids (>1.2 microm) and effectively trap fine (<63 microm) sediments. The waters were generally oxidising with neutral to basic pH which may have favoured metal partitioning to the solid phase. The distribution pattern for Zn and Cu in fine sediments contrasted in two of the systems studied, indicating the complex behaviour of metals in wetlands. Further work will increase the data set and examine the adsorption behaviour of metals. These results will contribute to the development of future design criteria for the application of wetlands to road runoff treatment. PMID:11804157

  7. DNA Mismatch Repair

    PubMed Central

    MARINUS, M. G.

    2014-01-01

    DNA mismatch repair functions to correct replication errors in newly synthesized DNA and to prevent recombination between related, but not identical (homeologous), DNA sequences. The mechanism of mismatch repair is best understood in Escherichia coli and is the main focus of this review. The early genetic studies of mismatch repair are described as a basis for the subsequent biochemical characterization of the system. The effects of mismatch repair on homologous and homeologous recombination are described. The relationship of mismatch repair to cell toxicity induced by various drugs is included. The VSP (Very Short Patch) repair system is described in detail. PMID:26442827

  8. A new fractional-order sliding mode controller via a nonlinear disturbance observer for a class of dynamical systems with mismatched disturbances.

    PubMed

    Pashaei, Shabnam; Badamchizadeh, Mohammadali

    2016-07-01

    This paper investigates the stabilization and disturbance rejection for a class of fractional-order nonlinear dynamical systems with mismatched disturbances. To fulfill this purpose a new fractional-order sliding mode control (FOSMC) based on a nonlinear disturbance observer is proposed. In order to design the suitable fractional-order sliding mode controller, a proper switching surface is introduced. Afterward, by using the sliding mode theory and Lyapunov stability theory, a robust fractional-order control law via a nonlinear disturbance observer is proposed to assure the existence of the sliding motion in finite time. The proposed fractional-order sliding mode controller exposes better control performance, ensures fast and robust stability of the closed-loop system, eliminates the disturbances and diminishes the chattering problem. Finally, the effectiveness of the proposed fractional-order controller is depicted via numerical simulation results of practical example and is compared with some other controllers. PMID:27108564

  9. Pattern recognition control outperforms conventional myoelectric control in upper limb patients with targeted muscle reinnervation.

    PubMed

    Hargrove, Levi J; Lock, Blair A; Simon, Ann M

    2013-01-01

    Pattern recognition myoelectric control shows great promise as an alternative to conventional amplitude based control to control multiple degree of freedom prosthetic limbs. Many studies have reported pattern recognition classification error performances of less than 10% during offline tests; however, it remains unclear how this translates to real-time control performance. In this contribution, we compare the real-time control performances between pattern recognition and direct myoelectric control (a popular form of conventional amplitude control) for participants who had received targeted muscle reinnervation. The real-time performance was evaluated during three tasks; 1) a box and blocks task, 2) a clothespin relocation task, and 3) a block stacking task. Our results found that pattern recognition significantly outperformed direct control for all three performance tasks. Furthermore, it was found that pattern recognition was configured much quicker. The classification error of the pattern recognition systems used by the patients was found to be 16% ±(1.6%) suggesting that systems with this error rate may still provide excellent control. Finally, patients qualitatively preferred using pattern recognition control and reported the resulting control to be smoother and more consistent. PMID:24110008

  10. FINE PARTICLE CONTROL TECHNOLOGY: CONVENTIONAL AND NOVEL DEVICES

    EPA Science Inventory

    This paper reviews the development of technology in fine particle control. Electrostatic precipitators (ESPs), scrubbers, fabric filters, and novel devices were assessed to determine their efficiency. ESPs were found to be excellent in controlling fine particles: with an efficien...

  11. 40 CFR 450.23 - Effluent limitations reflecting the best conventional pollutant control technology (BCT).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through... best conventional pollutant control technology (BCT). 450.23 Section 450.23 Protection of Environment... pollutant control technology (BCT). The effluent limitations are described at § 450.21....

  12. Altering Hydrophobic Sequence Lengths Shows That Hydrophobic Mismatch Controls Affinity for Ordered Lipid Domains (Rafts) in the Multitransmembrane Strand Protein Perfringolysin O*

    PubMed Central

    Lin, Qingqing; London, Erwin

    2013-01-01

    The hypothesis that mismatch between transmembrane (TM) length and bilayer width controls TM protein affinity for ordered lipid domains (rafts) was tested using perfringolysin O (PFO), a pore-forming cholesterol-dependent cytolysin. PFO forms a multimeric barrel with many TM segments. The properties of PFO mutants with lengthened or shortened TM segments were compared with that of PFO with wild type TM sequences. Both mutant and wild type length PFO exhibited cholesterol-dependent membrane insertion. Maximal PFO-induced pore formation occurred in vesicles with wider bilayers for lengthened TM segments and in thinner bilayers for shortened TM segments. In diC18:0 phosphatidylcholine (PC)/diC14:1 PC/cholesterol vesicles, which form ordered domains with a relatively thick bilayer and disordered domains with a relatively thin bilayer, affinity for ordered domains was greatest with lengthened TM segments and least with shortened TM segments as judged by FRET. Similar results were observed by microscopy in giant vesicles containing sphingomyelin in place of diC18:0 PC. In contrast, in diC16:0 PC/diC14:0 PC/diC20:1 PC/cholesterol vesicles, which should form ordered domains with a relatively thin bilayer and disordered domains with a relatively thick bilayer, relative affinity for ordered domains was greatest with shortened TM segments and least with lengthened TM segments. The inability of multi-TM segment proteins (unlike single TM segment proteins) to adapt to mismatch by tilting may explain the sensitivity of raft affinity to mismatch. The difference in width sensitivity for single and multi-TM helix proteins may link raft affinity to multimeric state and thus control the assembly of multimeric TM complexes in rafts. PMID:23150664

  13. Spread of arbitrary conventions among chimpanzees: a controlled experiment.

    PubMed

    Bonnie, Kristin E; Horner, Victoria; Whiten, Andrew; de Waal, Frans B M

    2007-02-01

    Wild chimpanzees (Pan troglodytes) have a rich cultural repertoire--traditions common in some communities are not present in others. The majority of reports describe functional, material traditions, such as tool use. Arbitrary conventions have received far less attention. In the same way that observations of material culture in wild apes led to experiments to confirm social transmission and identify underlying learning mechanisms, experiments investigating how arbitrary habits or conventions arise and spread within a group are also required. The few relevant experimental studies reported thus far have relied on cross-species (i.e. human-ape) interaction offering limited ecological validity, and no study has successfully generated a tradition not involving tool use in an established group. We seeded one of two rewarded alternative endpoints to a complex sequence of behaviour in each of two chimpanzee groups. Each sequence spread in the group in which it was seeded, with many individuals unambiguously adopting the sequence demonstrated by a group member. In one group, the alternative sequence was discovered by a low ranking female, but was not learned by others. Since the action-sequences lacked meaning before the experiment and had no logical connection with reward, chimpanzees must have extracted both the form and benefits of these sequences through observation of others. PMID:17164200

  14. 40 CFR 434.74 - Effluent limitations attainable by application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Effluent limitations attainable by application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32 and 434.72(b)(2), a pre-existing discharge must comply... application of the best conventional pollutant control technology (BCT). 434.74 Section 434.74 Protection...

  15. 40 CFR 450.23 - Effluent limitations reflecting the best conventional pollutant control technology (BCT).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... limitations reflecting the best conventional pollutant control technology (BCT). Except as provided in 40 CFR... best conventional pollutant control technology (BCT). 450.23 Section 450.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) CONSTRUCTION...

  16. 40 CFR 450.23 - Effluent limitations reflecting the best conventional pollutant control technology (BCT).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... limitations reflecting the best conventional pollutant control technology (BCT). Except as provided in 40 CFR... best conventional pollutant control technology (BCT). 450.23 Section 450.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) CONSTRUCTION...

  17. 40 CFR 450.23 - Effluent limitations reflecting the best conventional pollutant control technology (BCT).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... limitations reflecting the best conventional pollutant control technology (BCT). Except as provided in 40 CFR... best conventional pollutant control technology (BCT). 450.23 Section 450.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) CONSTRUCTION...

  18. 40 CFR 450.23 - Effluent limitations reflecting the best conventional pollutant control technology (BCT).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through... best conventional pollutant control technology (BCT). 450.23 Section 450.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONSTRUCTION AND DEVELOPMENT...

  19. 40 CFR 434.84 - Effluent limitations attainable by application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... application of the best conventional pollutant control technology (BCT). 434.84 Section 434.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) COAL... pollutant control technology (BCT)....

  20. High mobility AlGaN/GaN heterostructures grown on Si substrates using a large lattice-mismatch induced stress control technology

    SciTech Connect

    Cheng, Jianpeng; Yang, Xuelin Sang, Ling; Guo, Lei; Hu, Anqi; Xu, Fujun; Tang, Ning; Wang, Xinqiang; Shen, Bo

    2015-04-06

    A large lattice-mismatch induced stress control technology with a low Al content AlGaN layer has been used to grow high quality GaN layers on 4-in. Si substrates. The use of this technology allows for high mobility AlGaN/GaN heterostructures with electron mobility of 2040 cm{sup 2}/(V·s) at sheet charge density of 8.4 × 10{sup 12 }cm{sup −2}. Strain relaxation and dislocation evolution mechanisms have been investigated. It is demonstrated that the large lattice mismatch between the low Al content AlGaN layer and AlN buffer layer could effectively promote the edge dislocation inclination with relatively large bend angles and therefore significantly reduce the dislocation density in the GaN epilayer. Our results show a great potential for fabrication of low-cost and high performance GaN-on-Si power devices.

  1. Robust integral variable structure controller and pulse-width pulse-frequency modulated input shaper design for flexible spacecraft with mismatched uncertainty/disturbance.

    PubMed

    Hu, Qinglei

    2007-10-01

    This paper presents a dual-stage control system design method for the flexible spacecraft attitude maneuvering control by use of on-off thrusters and active vibration control by input shaper. In this design approach, attitude control system and vibration suppression were designed separately using lower order model. As a stepping stone, an integral variable structure controller with the assumption of knowing the upper bounds of the mismatched lumped perturbation has been designed which ensures exponential convergence of attitude angle and angular velocity in the presence of bounded uncertainty/disturbances. To reconstruct estimates of the system states for use in a full information variable structure control law, an asymptotic variable structure observer is also employed. In addition, the thruster output is modulated in pulse-width pulse-frequency so that the output profile is similar to the continuous control histories. For actively suppressing the induced vibration, the input shaping technique is used to modify the existing command so that less vibration will be caused by the command itself, which only requires information about the vibration frequency and damping of the closed-loop system. The rationale behind this hybrid control scheme is that the integral variable structure controller can achieve good precision pointing, even in the presence of uncertainties/disturbances, whereas the shaped input attenuator is applied to actively suppress the undesirable vibrations excited by the rapid maneuvers. Simulation results for the spacecraft model show precise attitude control and vibration suppression. PMID:17706218

  2. Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output

    PubMed Central

    Wefelmeyer, Winnie; Cattaert, Daniel; Burrone, Juan

    2015-01-01

    The axon initial segment (AIS) is a structure at the start of the axon with a high density of sodium and potassium channels that defines the site of action potential generation. It has recently been shown that this structure is plastic and can change its position along the axon, as well as its length, in a homeostatic manner. Chronic activity-deprivation paradigms in a chick auditory nucleus lead to a lengthening of the AIS and an increase in neuronal excitability. On the other hand, a long-term increase in activity in dissociated rat hippocampal neurons results in an outward movement of the AIS and a decrease in the cell’s excitability. Here, we investigated whether the AIS is capable of undergoing structural plasticity in rat hippocampal organotypic slices, which retain the diversity of neuronal cell types present at postnatal ages, including chandelier cells. These interneurons exclusively target the AIS of pyramidal neurons and form rows of presynaptic boutons along them. Stimulating individual CA1 pyramidal neurons that express channelrhodopsin-2 for 48 h leads to an outward shift of the AIS. Intriguingly, both the pre- and postsynaptic components of the axo-axonic synapses did not change position after AIS relocation. We used computational modeling to explore the functional consequences of this partial mismatch and found that it allows the GABAergic synapses to strongly oppose action potential generation, and thus downregulate pyramidal cell excitability. We propose that this spatial arrangement is the optimal configuration for a homeostatic response to long-term stimulation. PMID:26195803

  3. 78 FR 64014 - Importer of Controlled Substances; Notice of Application; United States Pharmacopeial Convention

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-25

    ..., 40 FR 43745-46, all applicants for registration to import a basic class of any controlled substance... Pharmacopeial Convention Pursuant to Title 21 Code of Federal Regulations 1301.34 (a), this is notice that on July 31, 2013, United States Pharmacopeial Convention, 12601 Twinbrook Parkway, Rockville,...

  4. An economic comparison of biological and conventional control strategies for whiteflies (Homoptera: Aleyrodidae) in greenhouse poinsettias.

    PubMed

    Stevens, T J; Kilmer, R L; Glenn, S J

    2000-06-01

    The objective of this study was to evaluate the costs of biologically controlling infestations of silverleaf whitefly, Bemisia argentifolii Bellows & Perring, in New England greenhouse operations on poinsettia, Euphorbia pulcherrima Wild, ex Koltz, using the parasitic wasp Encarsia formosa Gahan (Nile Delta strain). Partial budget analysis was used to compare costs for conventional verses biological control regimens. Four alternative whitefly control budgets are developed; two conventional chemical-based control budgets formulated with and without the use of imidacloprid, and two biological control budgets which demonstrate the impact of possibly greater pest monitoring efforts necessary to implement this type strategy successfully. The analysis shows that biological whitefly control costs were > 300% greater than conventional chemical-based control strategy costs. Most of this increase is caused by the higher costs of Encarsia formosa as the material control input. If monitoring costs are held constant across different strategies, labor costs actually decline for biological control. This is because of a significant reduction in the number of control applications made and the relatively lower cost of applying E. formosa. If more extensive monitoring efforts are required to implement biological control successfully, labor costs increase by 56% over the conventional pre-imidacloprid regimen. Based on these results, the authors conclude that cheaper and more reliable means of producing E. formosa must be developed before this strategy will become economically viable for commercial poinsettia greenhouse production. PMID:10902307

  5. A Jobs Mismatch. Commentary

    ERIC Educational Resources Information Center

    Marina, Brenda L. H.

    2011-01-01

    In the article "A Jobs Mismatch", Jaschik has compiled the findings of a new report that was released by the Georgetown University Center on Education and the Workforce. The Georgetown University report claims that there is a severe shortage of college graduates in America, and that this shortage has the United States on a "collision course with…

  6. Decreased Functional Diversity and Biological Pest Control in Conventional Compared to Organic Crop Fields

    PubMed Central

    Krauss, Jochen; Gallenberger, Iris; Steffan-Dewenter, Ingolf

    2011-01-01

    Organic farming is one of the most successful agri-environmental schemes, as humans benefit from high quality food, farmers from higher prices for their products and it often successfully protects biodiversity. However there is little knowledge if organic farming also increases ecosystem services like pest control. We assessed 30 triticale fields (15 organic vs. 15 conventional) and recorded vascular plants, pollinators, aphids and their predators. Further, five conventional fields which were treated with insecticides were compared with 10 non-treated conventional fields. Organic fields had five times higher plant species richness and about twenty times higher pollinator species richness compared to conventional fields. Abundance of pollinators was even more than one-hundred times higher on organic fields. In contrast, the abundance of cereal aphids was five times lower in organic fields, while predator abundances were three times higher and predator-prey ratios twenty times higher in organic fields, indicating a significantly higher potential for biological pest control in organic fields. Insecticide treatment in conventional fields had only a short-term effect on aphid densities while later in the season aphid abundances were even higher and predator abundances lower in treated compared to untreated conventional fields. Our data indicate that insecticide treatment kept aphid predators at low abundances throughout the season, thereby significantly reducing top-down control of aphid populations. Plant and pollinator species richness as well as predator abundances and predator-prey ratios were higher at field edges compared to field centres, highlighting the importance of field edges for ecosystem services. In conclusion organic farming increases biodiversity, including important functional groups like plants, pollinators and predators which enhance natural pest control. Preventative insecticide application in conventional fields has only short-term effects on aphid

  7. Conventional forces and arms control: Technology and strategy in a changing world

    SciTech Connect

    Pilat, J.F.; White, P.C.

    1990-06-01

    To address the implications of changes for future roles of conventional forces and to assess the technology implications of future strategies, force requirements, and conventional arms control agreements, the Center for National Security Studies in cooperation with the Defense Research and Applications Directorate of the Los Alamos National Laboratory held a conference on Conventional Forces and Arms Control: Technology and Strategy in a Changing World'' at Los Alamos from September 25--27, 1989. The distinguished participants from government, industry, and academia in the United States and Western Europe addressed such issues as: What are the implications of geopolitical and technological trends for international security and stability How will these global changes affect US and allied strategies and force structure, especially the requirements for conventional, nonnuclear forces What will be the role of and rationale for conventional forces in the context of current and prospective allied security requirements How can the West assure it will have the forces necessary for its security How will technological developments influence the structure of tomorrow's conventional forces What impacts will arms reductions have on future systems and force structures What are the prospects for the development and deployment in weapon systems of future conventional military technologies, in light of existing and potential political, economic, bureaucratic, and other impediments

  8. Civil society and the negotiation of the Framework Convention on Tobacco Control

    PubMed Central

    MAMUDU, H. M.

    2008-01-01

    Tobacco control civil society organisations mobilised to influence countries during the negotiation of the World Health Organization (WHO) Framework Convention on Tobacco Control (FCTC) between 1999 and 2003. Tobacco control civil society organisations and coalitions around the world embraced the idea of an international tobacco control treaty and came together as the Framework Convention Alliance (FCA), becoming an important non-state actor within the international system of tobacco control. Archival documents and interviews demonstrate that the FCA successfully used strategies including publication of a newsletter, shaming, symbolism and media advocacy to influence policy positions of countries during the FCTC negotiation. The FCA became influential in the negotiation process by mobilising tobacco control civil society organisations and resources with the help of the Internet and framing the tobacco control discussion around global public health. PMID:19333806

  9. Nonlinear disturbance observer-based control for multi-input multi-output nonlinear systems subject to mismatching condition

    NASA Astrophysics Data System (ADS)

    Yang, Jun; Li, Shihua; Chen, Wen-Hua

    2012-08-01

    For a multi-input multi-output (MIMO) nonlinear system, the existing disturbance observer-based control (DOBC) only provides solutions to those whose disturbance relative degree (DRD) is higher than or equal to its input relative degree. By designing a novel disturbance compensation gain matrix, a generalised nonlinear DOBC method is proposed in this article to solve the disturbance attenuation problem of the MIMO nonlinear system with arbitrary DRD. It is shown that the disturbances are able to be removed from the output channels by the proposed method with appropriately chosen control parameters. The property of nominal performance recovery, which is the major merit of the DOBCs, is retained with the proposed method. The feasibility and effectiveness of the proposed method are demonstrated by simulation studies of both the numerical and application examples.

  10. Participation of non-conventional energy resources in power system frequency control

    NASA Astrophysics Data System (ADS)

    Aghazadeh Tabrizi, Mehriar

    Frequency control is one of the key issues in designing, planning and reliably operating a power system and is becoming more challenging as new complexities and uncertainties are introduced into the modern power systems. Traditionally, power system frequency has been controlled using conventional generation units' capabilities namely inertial, primary and secondary frequency responses. Limited fossil-based fuel resources, ever-increasing energy consumption and rising public awareness for environmental protection have created growing interest in use of non-conventional energy resources such as Wind Generation Resources (WGRs) and Solar Generation Resources (SGRs) which have unfavorable characteristics in comparison with conventional generation units such as lack of frequency response. The more conventional generation units are replaced by these resources, the more challenges power system operators will face in terms of power system frequency control. These challenges are further compounded due to less system inertia during off-peak hours or within small power systems. This dissertation mainly focuses on participation of SGRs and Interior Permanent Magnet Synchronous Generator (IPMSG) based WGRs in power system frequency control. Detailed information regarding dynamic modeling of power system including conventional generation units, SGRs and IPMSG based WGRs is provided. The frequency response of conventional generation units is compared with that of SGRs and IPMSG based WGRs. The control systems associated with IPMSG based WGR and SGR are modified in order to improve their frequency response capabilities. The effectiveness of the proposed control strategies is evaluated and confirmed via MATLAB based time-domain simulations for different scenarios. Moreover, application of Battery Energy Storage Systems (BESSs) in power system frequency regulation is discussed. The detailed dynamic model of BESSs is utilized to develop a simplified model suitable for Automatic

  11. Integration of Kinetic Control and Lattice Mismatch To Synthesize Pd@AuCu Core-Shell Planar Tetrapods with Size-Dependent Optical Properties.

    PubMed

    Meng, Min; Fang, Zhicheng; Zhang, Chao; Su, Hongyang; He, Rong; Zhang, Renpeng; Li, Hongliang; Li, Zhi-Yuan; Wu, Xiaojun; Ma, Chao; Zeng, Jie

    2016-05-11

    Planar nanocrystals with multiple branches exhibit unique localized surface plasmon resonance properties and great promise in optical applications. Here, we report an aqueous synthesis of Pd@AuCu core-shell planar tetrapods through preferential overgrowth on Pd cubic seeds. The large lattice mismatch between the Pd core and the AuCu shell is the key to induce the formation of branches under sluggish reduction kinetics. Meanwhile, the capping effect of cetyltrimethylammonium chloride on the {100} facets of Pd cubes with an aspect ratio of 1.2 can determine the growth direction of AuCu branches to form a planar structure. Through simply varying the amounts of Pd cubic seeds, the sizes of products can be well-controlled in the range from 33 to 70 nm. With the manipulation of sizes, the peak position of in-plane dipole resonance can be adjusted from visible to near-infrared region. Due to the presence of tips and edges in the branches, planar tetrapods exhibited excellent surface-enhanced Raman scattering performance with an enhancement factor up to 9.0 × 10(3) for 70 nm Pd@AuCu planar tetrapods. PMID:27074129

  12. Simulation of traffic flow and control using conventional, fuzzy, and adaptive methods

    SciTech Connect

    Bisset, K.R.; Kelsey, R.L.

    1992-01-01

    This paper describes the graphical simulation of a traffic environment. The environment includes streets leading to an intersection, the intersection, vehicle traffic, and signal lights in the intersection controlled by different methods. The simulation allows for the study of parameters affecting traffic environments and the study of different control strategies for traffic signal lights, including conventional, fuzzy, and adaptive control methods. Realistic traffic environments are simulated including a cross intersection, with one or more lanes of traffic in each direction, with and without turn lanes. Vehicle traffic patterns are a mixture of cars going straight and making right or left turns. The free velocities of vehicles follow a normal distribution with a mean of the posted'' speed limit. Actual velocities depend on such factors as the proximity and velocity of surrounding traffic, approaches to intersections, and human response time. The simulation proves the be a useful tool for evaluating controller methods. Preliminary results show that larger quantities of traffic are handled'' by fuzzy control methods then by conventional control methods. Also, the average time spent waiting in traffic decreases with the use of fuzzy control versus conventional control.

  13. Simulation of traffic flow and control using conventional, fuzzy, and adaptive methods

    SciTech Connect

    Bisset, K.R.; Kelsey, R.L.

    1992-06-01

    This paper describes the graphical simulation of a traffic environment. The environment includes streets leading to an intersection, the intersection, vehicle traffic, and signal lights in the intersection controlled by different methods. The simulation allows for the study of parameters affecting traffic environments and the study of different control strategies for traffic signal lights, including conventional, fuzzy, and adaptive control methods. Realistic traffic environments are simulated including a cross intersection, with one or more lanes of traffic in each direction, with and without turn lanes. Vehicle traffic patterns are a mixture of cars going straight and making right or left turns. The free velocities of vehicles follow a normal distribution with a mean of the ``posted`` speed limit. Actual velocities depend on such factors as the proximity and velocity of surrounding traffic, approaches to intersections, and human response time. The simulation proves the be a useful tool for evaluating controller methods. Preliminary results show that larger quantities of traffic are ``handled`` by fuzzy control methods then by conventional control methods. Also, the average time spent waiting in traffic decreases with the use of fuzzy control versus conventional control.

  14. Wavelength mismatch effect in electromagnetically induced absorption

    NASA Astrophysics Data System (ADS)

    Bharti, Vineet; Wasan, Ajay; Natarajan, Vasant

    2016-07-01

    We present a theoretical investigation of the phenomenon of electromagnetically induced absorption (EIA) in a 4-level system consisting of vee and ladder subsystems. The four levels are coupled using one weak probe field, and two strong control fields. We consider an experimental realization using energy levels of Rb. This necessitates dealing with different conditions of wavelength mismatch-near-perfect match where all three wavelengths are approximately equal; partial mismatch where the wavelength of one control field is less than the other fields; and complete mismatch where all three wavelengths are unequal. We present probe absorption profiles with Doppler averaging at room temperature to account for experiments in a room temperature Rb vapor cell. Our analysis shows that EIA resonances can be studied using Rydberg states excited with diode lasers.

  15. Cooperative Mmonitoring Center Occasional Paper/5: Propspects of Conventional Arms Control in South Asia

    SciTech Connect

    Gupta, Amit; Kamal, Nazir

    1998-11-01

    The intensely adversarial relationship between India and Pakistan is marked by military rivalry, mutual distrust, and suspicion. The most dividing disagreement has been over the Kashmir region. An inability to discuss the Kashmir issue has prevented discussion on other important issues. Since there is little prospect of detente, at least in the near-term, the question is whether this rivalry can be contained by other means, such as arms control approaches. Conventional arms control has been applied flexibly and successfully in some regions to reduce threat-perceptions and achieve reassuring military stability. Some lessons from other international models might be applied to the India/Pakistan context. This paper discusses the status of conventional arms control in South Asia, the dominant Indian and Pakistani perceptions about arms control, the benefits that could be derived from arms control, as well as the problems and prospects of arms control. It also discusses existing conventional arms control agreements at the regional and global levels as well as the potential role of cooperative monitoring technology.

  16. Ground Truthing the ‘Conventional Wisdom’ of Lead Corrosion Control Using Mineralogical Analysis

    EPA Science Inventory

    For drinking water distribution systems (DWDS) with lead-bearing plumbing materials some form of corrosion control is typically necessary, with the goal of mitigating lead release by forming adherent, stable corrosion scales composed of low-solubility mineral phases. Conventional...

  17. Ground Truthing the 'Conventional Wisdom' of Lead Corrosion Control Using Mineralogical Analysis

    EPA Science Inventory

    For drinking water distribution systems (DWDS) with lead-bearing plumbing materials some form of corrosion control is typically necessary, with the goal of mitigating lead release by forming adherent, stable corrosion scales composed of low-solubility mineral phases. Conventional...

  18. SHIFTS IN VINEYARD WEED SEED BANK COMPOSITION IN REPONSE TO ORGANIC AND CONVENTIONAL WEED CONTROL PRACTICES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The aim of this research was to compare the organic weed control practice, soil cultivation, to the conventional practice, applications of the herbicide, glyphosate, in terms of their effects on weed seed bank in a vineyard system. The experiment was conducted in a commercial winegrape vineyard in t...

  19. 40 CFR 434.74 - Effluent limitations attainable by application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... technology (BCT). Except as provided in 40 CFR 125.30 through 125.32 and 434.72(b)(2), a pre-existing... application of the best conventional pollutant control technology (BCT). 434.74 Section 434.74 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  20. Frontal and temporal sources of mismatch negativity in healthy controls, patients at onset of schizophrenia in adolescence and others at 15 years after onset.

    PubMed

    Oknina, L B; Wild-Wall, N; Oades, R D; Juran, S A; Röpcke, B; Pfueller, U; Weisbrod, M; Chan, E; Chen, E Y H

    2005-07-01

    Mismatch negativity (MMN) is an event-related potential measure of auditory change detection. It is widely reported to be smaller in patients with schizophrenia and may not improve along with otherwise successful clinical treatment. The main aim of this report is to explore ways of measuring and presenting four features of frequency-deviant MMN dipole sources (dipole moment, peak latency, brain location and orientation) and to relate these to the processes of psychopathology and illness progression. Data from early onset patients (EOS) at the start of the illness in adolescence, and others who had their first break in adolescence 15 years ago (S-15Y) were compared with two groups of age-matched healthy controls (C-EOS, C-15Y). A four-source model fitted the MMN waveform recorded from all four groups, whether MMN amplitude was more (EOS) or less (S-15Y) reduced. The locations were in the left superior temporal and anterior cingulate gyri, right superior temporal and inferior/mid frontal cortices. Dipole latencies confirmed a bottom-up sequence of processing and dipole moments were larger in the temporal lobes and on the left. Patients showed small dipole location changes that were more marked in the S-15Y than the EOS group (more rostral for the left anterior cingulate, more caudal for the right mid-frontal dipole) consistent with illness progression. The modelling of MMN dipole sources on brain atlas and anatomical images suggests that there is a degree of dissociation during illness between small progressive anatomical changes and some functional recovery indexed by scalp recordings from patients with an onset in adolescence 15 years before compared to adolescents in their first episode. PMID:15927796

  1. Thermal control of a lidar laser system using a non-conventional ram air heat exchanger

    NASA Technical Reports Server (NTRS)

    Killough, Brian D.; Alexander, William, Jr.; Swofford, Doyle P.

    1990-01-01

    This paper describes the analysis and performance testing of a uniquely designed external heat exchanger. The heat exchanger is attached externally to an aircraft and is used to cool a laser system within the fuselage. Estimates showed insufficient cooling capacity with a conventional staggered tube array in the limited space available. Thus, a non-conventional design wes developed with larger tube and fin area exposed to the ram air to increase the heat transfer performance. The basic design consists of 28 circular finned aluminum tubes arranged in two parallel banks. Wind tunnel tests were performed to simulate air and liquid flight conditions for the non-conventional parallel bank arrangement and the conventional staggered tube arrangement. Performance comparisons of each of the two designs are presented. Test results are used in a computer model of the heat exchanger to predict the operating performance for the entire flight profile. These analyses predict significantly improved performance over the conventional design and show adequate thermal control margins.

  2. ELECTRICAL AND ELECTRONIC INDUSTRIAL CONTROL. A-C CONVENTIONAL MAGNETIC MOTOR CONTROL, PART I, UNIT 5, ASSIGNMENTS.

    ERIC Educational Resources Information Center

    SUTTON, MACK C.

    THIS GUIDE IS FOR INDIVIDUAL STUDENT USE IN STUDYING ALTERNATING CURRENT CONVENTIONAL MAGNETIC MOTOR CONTROL IN ELECTRICAL-ELECTRONIC PROGRAMS. IT WAS DEVELOPED BY AN INSTRUCTIONAL MATERIALS SPECIALIST AND ADVISERS. EACH OF THE 10 ASSIGNMENT SHEETS PROVIDES THE LESSON SUBJECT, PURPOSE, INTRODUCTORY INFORMATION, STUDY REFERENCES, SUPPLEMENTARY…

  3. ELECTRICAL AND ELECTRONIC INDUSTRIAL CONTROL. A-C CONVENTIONAL MAGNETIC MOTOR CONTROL, PART I, UNIT 5, INSTRUCTOR'S GUIDE.

    ERIC Educational Resources Information Center

    SUTTON, MACK C.

    THIS GUIDE IS FOR TEACHER USE IN DIRECTING INDIVIDUAL STUDY OF ALTERNATING CURRENT CONVENTIONAL MAGNETIC MOTOR CONTROL IN ELECTRICAL-ELECTRONIC PROGRAMS. IT WAS DEVELOPED BY AN INSTRUCTIONAL MATERIALS SPECIALIST AND ADVISERS. EACH OF THE 10 INSTRUCTOR'S SHEETS GIVES THE LESSON SUBJECT, PURPOSE, INTRODUCTORY INFORMATION, REFERENCES, AND…

  4. ELECTRICAL AND ELECTRONIC INDUSTRIAL CONTROL. A-C CONVENTIONAL MAGNETIC MOTOR CONTROL, PART II, UNIT 6, INSTRUCTOR'S GUIDE.

    ERIC Educational Resources Information Center

    SUTTON, MACK C.

    THIS GUIDE IS FOR TEACHER USE IN DIRECTING INDIVIDUAL STUDY OF ALTERNATING CURRENT CONVENTIONAL MAGNETIC MOTOR CONTROL IN ELECTRICAL-ELECTRONIC PROGRAMS. IT WAS DEVELOPED BY AN INSTRUCTIONAL MATERIALS SPECIALIST AND ADVISERS. EACH OF THE 10 INSTRUCTOR'S SHEETS GIVES THE LESSON SUBJECT, PURPOSE, INTRODUCTORY INFORMATION, REFERENCES, SUPPLEMENTARY…

  5. ELECTRICAL AND ELECTRONIC INDUSTRIAL CONTROL. A-C CONVENTIONAL MAGNETIC MOTOR CONTROL, PART II, UNIT 6, ASSIGNMENTS.

    ERIC Educational Resources Information Center

    SUTTON, MACK C.

    THIS STUDY GUIDE IS FOR INDIVIDUAL STUDENT USE IN STUDYING ALTERNATING CURRENT CONVENTIONAL MAGNETIC MOTOR CONTROL IN ELECTRICAL-ELECTRONIC PROGRAMS. IT WAS DEVELOPED BY AN INSTRUCTIONAL MATERIALS SPECIALIST AND ADVISERS. EACH OF THE 10 ASSIGNMENT SHEETS PROVIDES THE LESSON SUBJECT, PURPOSE, INTRODUCTORY INFORMATION, STUDY REFERENCES,…

  6. An Experimental Database for Conventional and Alternate Control Concepts on the HSR 1.675% Reference H Model

    NASA Technical Reports Server (NTRS)

    McMillin, Naomi; Allen, Jerry; Erickson, Gary; Campbell, Jim; Mann, Mike; Kubiatko, Paul; Yingling, David; Mason, Charlie

    1999-01-01

    The objective was to experimentally evaluate the longitudinal and lateral-directional stability and control characteristics of the Reference H configuration at supersonic and transonic speeds. A series of conventional and alternate control devices were also evaluated at supersonic and transonic speeds. A database on the conventional and alternate control devices was to be created for use in the HSR program.

  7. 40 CFR 438.13 - Effluent limitations attainable by application of the best control technology for conventional...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... application of the best control technology for conventional pollutants (BCT). Except as provided at 40 CFR 125... of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  8. Conventional Middle East arms control: Impact of the end of the cold war. Study project report

    SciTech Connect

    Johnson, L.L.; Johnsen, W.T.

    1993-03-31

    The end of the Gulf War brought to the forefront concern for dangers posed by unrestrained militarization of the Middle East. In response, on 29 May 1991 President Bush unveiled a comprehensive Middle East arms control policy in a speech at the U.S. Air Force Academy. A key element of the policy banned the sale of the most dangerous conventional weapons to the region. Although the major arms suppliers (which also happen to be the five permanent members of the U.N. Security Council) have held a series of high level meetings to discuss options for restricting sales to the region, all continue conventional arms transfers to the Middle East and are likely to continue to do so. This paper contends that the end of the Cold War put additional economic pressure on the major suppliers to export arms to the Middle East; and, their interests are so compelling that the suppliers are unlikely to support President Bush's proposal. This position is supported by analyzing the interests that influence major arms suppliers to sell arms abroad. The format for this analysis includes an assessment of: each country's interest in selling arms during the Cold War; the impact of the Cold War's end on those interests; and whether the post Cold War interests conflict with President Bush's conventional arms control proposal. The paper concludes with recommendations for US policy in the region.

  9. Composition of transgenic Volvariella volvacea tolerant to cold stress is equivalent to that of conventional control.

    PubMed

    Wang, Jie; Guo, Liqiong; Lin, Junfang

    2009-03-25

    Transgenic Volvariella volvacea strains, which are tolerant to cold stress, were generated by the stable insertion of antifreeze protein gene isolated from budworm into the genome of a conventional variety V23 of V. volvacea. As a part of the safety assessment program, transgenic V. volvacea strains were compared to a nontransgenic near-isogenic V. volvacea strain V23 grown contemporaneously by applying the principle of substantial equivalence. Compositional analyses were conducted by measuring proximates, amino acids, fatty acids, minerals, vitamins, 5'-nucleotides, nucleic acid, and antinutrients such as tannin and cyanide in V. volvacea strains harvested at egg-shaped stage. Results of the comparisons indicate that these transgenic strains are compositionally equivalent to the conventional control. PMID:19245205

  10. 40 CFR 412.44 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... limitations attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 412.44 Section 412.44 Protection...

  11. 40 CFR 442.12 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). 442.12 Section 442.12 Protection...

  12. 40 CFR 439.43 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 439.43 Section 439.43 Protection...

  13. 40 CFR 438.13 - Effluent limitations attainable by application of the best control technology for conventional...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best control technology for conventional pollutants (BCT). Except as provided at 40 CFR 125... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by application of the best control technology for conventional pollutants (BCT). 438.13 Section 438.13...

  14. 40 CFR 442.42 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 442.42 Section 442.42 Protection...

  15. 40 CFR 437.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32 or 437.20(b), any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 437.22 Section 437.22 Protection...

  16. 40 CFR 437.32 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32 or § 437.30(b), any existing point source subject to this subpart... application of the best conventional pollutant control technology (BCT). 437.32 Section 437.32 Protection...

  17. 40 CFR 412.32 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this... application of the best conventional pollutant control technology (BCT). 412.32 Section 412.32 Protection...

  18. 40 CFR 439.33 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). 439.33 Section 439.33 Protection...

  19. 40 CFR 437.12 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32 or 437.10(b), any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 437.12 Section 437.12 Protection...

  20. 40 CFR 442.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this... application of the best conventional pollutant control technology (BCT). 442.22 Section 442.22 Protection...

  1. 40 CFR 445.12 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). 445.12 Section 445.12 Protection...

  2. 40 CFR 439.23 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). 439.23 Section 439.23 Protection...

  3. 40 CFR 439.13 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). 439.13 Section 439.13 Protection...

  4. 40 CFR 445.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 445.22 Section 445.22 Protection...

  5. 40 CFR 444.14 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 444.14 Section 444.14 Protection...

  6. 40 CFR 437.43 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the application of the best conventional pollutant control technology (BCT). (a) Except as provided in 40 CFR 125.30 through 125.32 or 437.40(b), any existing facility subject to this subpart which... application of the best conventional pollutant control technology (BCT). 437.43 Section 437.43 Protection...

  7. 40 CFR 442.12 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any... application of the best conventional pollutant control technology (BCT). 442.12 Section 442.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  8. 40 CFR 442.32 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any... application of the best conventional pollutant control technology (BCT). 442.32 Section 442.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  9. Preharvest treatments with chitosan and other alternatives to conventional fungicides to control postharvest decay of strawberry.

    PubMed

    Feliziani, Erica; Landi, Lucia; Romanazzi, Gianfranco

    2015-11-01

    The effectiveness of the control of postharvest decay of strawberry (Fragaria × ananassa, 'Alba' and 'Romina' cvs.) fruit following field applications of chitosan, laminarin, extracts of Abies spp., Polygonum spp., and Saccharomyces spp., an organic acids and calcium combination, and benzothiadiazole, were compared with a fungicide strategy. These compounds were sprayed every 5 days on the strawberry canopy, from flowering to ripening, in 2012 and 2013. The treatments with alternative compounds provided ∼ 30% reduction in postharvest decay of strawberry compared to the water-treated controls, mainly against gray mold and Rhizopus rot, and without negatively affecting fruit color and firmness. Chitosan and benzothiadiazole were the most effective alternative treatments. Preharvest spraying with these alternative treatments can complement the use of conventional fungicides in the control of postharvest decay of strawberry fruit, especially when disease pressure is low. PMID:26256331

  10. Comparison of microsurgical and conventional open flap debridement: A randomized controlled trial

    PubMed Central

    Perumal, Meena Priya Bagavathy; Ramegowda, Aruna Dunthur; Lingaraju, Avinash Janaki; Raja, James Johnson

    2015-01-01

    Background: Residual calculus exists not only on teeth treated by scaling alone but also on teeth treated by flap surgery. Periodontal microsurgery enables more definite removal of calculus, atraumatic handling of tissues through optical magnification. The purpose of this study was to compare the clinical outcomes of microsurgery with conventional open flap debridement in patients with chronic periodontitis. Materials and Methods: Thirteen chronic periodontitis patients were randomly assigned for test (microsurgical) and control (conventional) open flap debridement in a split mouth design. At baseline, 3, 6 and 9 months the following clinical parameters were recorded: Probing pocket depth, relative attachment level, gingival recession, gingival bleeding index. Postoperative healing at 1-week by early healing index and pain scale for 7 days were assessed. Results: Paired t-test was used to compare means within the groups, and unpaired t-test was applied to compare the means of the two groups. At 3, 6 and 9 months postoperatively there was a significant reduction in gingival bleeding index, probing pocket depth, relative attachment level within both the groups and there was no significant difference between both the groups. Gingival margin level and gingival recession increased in both the groups, but it was not statistically significant. Early healing Index score of 1 was found in 85% of test sites and 28% of control sites. The mean pain scale was 0 in test site and 1.07 ± 0.75 in control site. Conclusions: In open flap debridement procedure, a microsurgical approach can substantially improve the early healing index and induce less postoperative pain compared with applying a conventional macroscopic approach. PMID:26392689

  11. China's position in negotiating the Framework Convention on Tobacco Control and the revised International Health Regulations.

    PubMed

    Huang, Y

    2014-02-01

    This paper examines China's position in the negotiations of the Framework Convention on Tobacco Control and the revised International Health Regulations. In particular, it explores three sets of factors shaping China's attitudes and actions in the negotiations: the aspiration to be a responsible power; concerns about sovereignty; and domestic political economy. In both cases, China demonstrated strong incentives to participate in the negotiation of legally binding international rules. Still, the sovereignty issue was a major, if not the biggest, concern for China when engaging in global health rule making. The two cases also reveal domestic political economy as an important factor in shaping China's position in international health negotiations. PMID:24370173

  12. Conventional vs. Tablet Computer-Based Patient Education following Lung Transplantation – A Randomized Controlled Trial

    PubMed Central

    Suhling, Hendrik; Rademacher, Jessica; Zinowsky, Imke; Fuge, Jan; Greer, Mark; Warnecke, Gregor; Smits, Jacqueline M.; Bertram, Anna; Haverich, Axel; Welte, Tobias; Gottlieb, Jens

    2014-01-01

    Background Accurate immunosuppression is of critical importance in preventing rejection, while avoiding toxicity following lung transplantation. The mainstay immunosuppressants are calcineurin inhibitors, which require regular monitoring due to interactions with other medications and diet. Adherence to immunosuppression and patient knowledge is vital and can be improved through patient education. Education using tablet-computers was investigated. Objective To compare tablet-PC education and conventional education in improving immunosuppression trough levels in target range 6 months after a single education. Secondary parameters were ratio of immunosuppression level measurements divided by per protocol recommended measurements, time and patient satisfaction regarding education. Design Single-centre, open labelled randomised controlled trial. Participants Patients >6 months after lung-transplantation with <50% of calcineurin inhibitor trough levels in target range. Intervention Tablet-pc education versus personal, nurse-led education. Measurements Calcineurin inhibitor levels in target range 6 months after education, level variability, interval adherence, knowledge and adherence was studied. As outcome parameter, renal function was measured and adverse events registered. Results Sixty-four patients were 1:1 randomised for either intervention. Levels of immunosuppression 6 months after education were equal (tablet-PC 58% vs. conventional 48%, p = 0.27), both groups improved in achieving a CNI trough level within target range by either education method (delta tablet-PC 29% vs. conventional 20%). In all patients, level variability decreased (−20.4%), whereas interval adherence remained unchanged. Knowledge about immunosuppression improved by 7% and compliance tests demonstrated universal improvements with no significant difference between groups. Conclusion Education is a simple, effective tool in improving adherence to immunosuppression. Tablet-PC education was

  13. Instrument Display Visual Angles for Conventional Aircraft and the MQ-9 Ground Control Station

    NASA Technical Reports Server (NTRS)

    Bendrick, Gregg A.; Kamine, Tovy Haber

    2008-01-01

    Aircraft instrument panels should be designed such that primary displays are in optimal viewing location to minimize pilot perception and response time. Human Factors engineers define three zones (i.e. "cones") of visual location: 1) "Easy Eye Movement" (foveal vision); 2) "Maximum Eye Movement" (peripheral vision with saccades), and 3) "Head Movement" (head movement required). Instrument display visual angles were measured to determine how well conventional aircraft (T-34, T-38, F- 15B, F-16XL, F/A-18A, U-2D, ER-2, King Air, G-III, B-52H, DC-10, B747-SCA) and the MQ-9 ground control station (GCS) complied with these standards, and how they compared with each other. Methods: Selected instrument parameters included: attitude, pitch, bank, power, airspeed, altitude, vertical speed, heading, turn rate, slip/skid, AOA, flight path, latitude, longitude, course, bearing, range and time. Vertical and horizontal visual angles for each component were measured from the pilot s eye position in each system. Results: The vertical visual angles of displays in conventional aircraft lay within the cone of "Easy Eye Movement" for all but three of the parameters measured, and almost all of the horizontal visual angles fell within this range. All conventional vertical and horizontal visual angles lay within the cone of "Maximum Eye Movement". However, most instrument vertical visual angles of the MQ-9 GCS lay outside the cone of "Easy Eye Movement", though all were within the cone of "Maximum Eye Movement". All the horizontal visual angles for the MQ-9 GCS were within the cone of "Easy Eye Movement". Discussion: Most instrument displays in conventional aircraft lay within the cone of "Easy Eye Movement", though mission-critical instruments sometimes displaced less important instruments outside this area. Many of the MQ-9 GCS systems lay outside this area. Specific training for MQ-9 pilots may be needed to avoid increased response time and potential error during flight.

  14. Optimal control strategies of eradicating invisible glioblastoma cells after conventional surgery.

    PubMed

    de Los Reyes V, Aurelio A; Jung, Eunok; Kim, Yangjin

    2015-05-01

    Glioblastoma, the most aggressive type of brain cancer, has median survival time of 1 year after diagnosis. It is characterized by alternating modes of rapid proliferation and aggressive invasion in response to metabolic stress in the microenvironment. A particular microRNA, miR-451, and its downstream signalling molecules, AMPK complex, are known to be key determinants in switching cell fate. These components form a core control system determining a balance between cell growth and migration which is regulated by fluctuating glucose levels in the microenvironment. An important factor from the treatment point of view is that low levels of glucose affect metabolism and activate cell migration through the miR-451-AMPK control system, creating 'invisible' migratory cells and making them inaccessible by conventional surgery. In this work, we apply optimal control theory to deal with the problem of maintaining upregulated miR-451 levels that prevent cell infiltration to surrounding brain tissue and thus induce localization of these cancer cells at the surgical site. The model also considers the effect of a drug that blocks inhibitive pathways of miR-451 from AMPK complex. Glucose infusion control and drug infusion control are chosen to represent dose rates of glucose and drug intravenous administrations, respectively. The characteristics of optimal control lead us to investigate the structure of optimal intravenous infusion regimen under various circumstances and predict best clinical outcomes with minimum expense possible. PMID:25833239

  15. Optimal control strategies of eradicating invisible glioblastoma cells after conventional surgery

    PubMed Central

    de los Reyes V, Aurelio A.; Jung, Eunok; Kim, Yangjin

    2015-01-01

    Glioblastoma, the most aggressive type of brain cancer, has median survival time of 1 year after diagnosis. It is characterized by alternating modes of rapid proliferation and aggressive invasion in response to metabolic stress in the microenvironment. A particular microRNA, miR-451, and its downstream signalling molecules, AMPK complex, are known to be key determinants in switching cell fate. These components form a core control system determining a balance between cell growth and migration which is regulated by fluctuating glucose levels in the microenvironment. An important factor from the treatment point of view is that low levels of glucose affect metabolism and activate cell migration through the miR-451-AMPK control system, creating ‘invisible’ migratory cells and making them inaccessible by conventional surgery. In this work, we apply optimal control theory to deal with the problem of maintaining upregulated miR-451 levels that prevent cell infiltration to surrounding brain tissue and thus induce localization of these cancer cells at the surgical site. The model also considers the effect of a drug that blocks inhibitive pathways of miR-451 from AMPK complex. Glucose infusion control and drug infusion control are chosen to represent dose rates of glucose and drug intravenous administrations, respectively. The characteristics of optimal control lead us to investigate the structure of optimal intravenous infusion regimen under various circumstances and predict best clinical outcomes with minimum expense possible. PMID:25833239

  16. Status of implementation of Framework Convention on Tobacco Control (FCTC) in Ghana: a qualitative study

    PubMed Central

    2010-01-01

    Background The Framework Convention on Tobacco Control (FCTC), a World Health Organization treaty, has now been ratified by over 165 countries. However there are concerns that implementing the Articles of the treaty may prove difficult, particularly in the developing world. In this study we have used qualitative methods to explore the extent to which the FCTC has been implemented in Ghana, a developing country that was 39th to ratify the FCTC, and identify barriers to effective FCTC implementation in low income countries. Methods Semi-structured interviews with 20 members of the national steering committee for tobacco control in Ghana, the official multi-disciplinary team with responsibility for tobacco control advocacy and policy formulation, were conducted. The Framework method for analysis and NVivo software were used to identify key issues relating to the awareness of the FCTC and the key challenges and achievements in Ghana to date. Results Interviewees had good knowledge of the content of the FCTC, and reported that although Ghana had no explicitly written policy on tobacco control, the Ministry of Health had issued several tobacco control directives before and since ratification. A national tobacco control bill has been drafted but has not been implemented. Challenges identified included the absence of a legal framework for implementing the FCTC, and a lack of adequate resources and prioritisation of tobacco control efforts, leading to slow implementation of the treaty. Conclusion Whilst Ghana has ratified the FCTC, there is an urgent need for action to pass a national tobacco control bill into law to enable it to implement the treaty, sustain tobacco control efforts and prevent Ghana's further involvement in the global tobacco epidemic. PMID:20043862

  17. [The Framework Convention for Tobacco Control (FCTC): a powerful public health instrument].

    PubMed

    Bovet, P; Cornuz, J; Paccaud, F

    2012-07-11

    The Framework Convention on Tobacco Control (FCTC) isa global and comprehensive legal framework for reducing demand for tobacco (e.g. price measures; ban on smoking in enclosed places; contents of tobacco products; packaging and labeling; advertising, promotion and sponsorship; liability, tobacco cessation, etc.) and supply (e.g. illicit trade; sales to/by minors, etc.). Adopted in 2003, the FCTC has been ratified by 174 countries so far. Switzerland has signed the treaty in 2004 but ratification will necessitate the implementation of stronger tobacco control measures at the national level. The FCTC is a priority of any strategy to reduce noncommunicable diseases in populations. Broad implementation of the FCTC has the potential to prevent a substantial proportion of the billion of tobacco-related deaths expected in the 21st century. PMID:22934470

  18. Instrument Display Visual Angles for Conventional Aircraft and the MQ-9 Ground Control Station

    NASA Technical Reports Server (NTRS)

    Kamine, Tovy Haber; Bendrick, Gregg A.

    2008-01-01

    Aircraft instrument panels should be designed such that primary displays are in optimal viewing location to minimize pilot perception and response time. Human Factors engineers define three zones (i.e. cones ) of visual location: 1) "Easy Eye Movement" (foveal vision); 2) "Maximum Eye Movement" (peripheral vision with saccades), and 3) "Head Movement (head movement required). Instrument display visual angles were measured to determine how well conventional aircraft (T-34, T-38, F- 15B, F-16XL, F/A-18A, U-2D, ER-2, King Air, G-III, B-52H, DC-10, B747-SCA) and the MQ-9 ground control station (GCS) complied with these standards, and how they compared with each other. Selected instrument parameters included: attitude, pitch, bank, power, airspeed, altitude, vertical speed, heading, turn rate, slip/skid, AOA, flight path, latitude, longitude, course, bearing, range and time. Vertical and horizontal visual angles for each component were measured from the pilot s eye position in each system. The vertical visual angles of displays in conventional aircraft lay within the cone of "Easy Eye Movement" for all but three of the parameters measured, and almost all of the horizontal visual angles fell within this range. All conventional vertical and horizontal visual angles lay within the cone of Maximum Eye Movement. However, most instrument vertical visual angles of the MQ-9 GCS lay outside the cone of Easy Eye Movement, though all were within the cone of Maximum Eye Movement. All the horizontal visual angles for the MQ-9 GCS were within the cone of "Easy Eye Movement". Most instrument displays in conventional aircraft lay within the cone of Easy Eye Movement, though mission-critical instruments sometimes displaced less important instruments outside this area. Many of the MQ-9 GCS systems lay outside this area. Specific training for MQ-9 pilots may be needed to avoid increased response time and potential error during flight. The learning objectives include: 1) Know three

  19. A comprehensive model to evaluate implementation of the world health organization framework convention of tobacco control

    PubMed Central

    Sarrafzadegan, Nizal; Kelishad, Roya; Rabiei, Katayoun; Abedi, Heidarali; Mohaseli, Khadijeh Fereydoun; Masooleh, Hasan Azaripour; Alavi, Mousa; Heidari, Gholamreza; Ghaffari, Mostafa; O’Loughlin, Jennifer

    2012-01-01

    Background: Iran is one of the countries that has ratified the World Health Organization Framework Convention of Tobacco Control (WHO-FCTC), and has implemented a series of tobacco control interventions including the Comprehensive Tobacco Control Law. Enforcement of this legislation and assessment of its outcome requires a dedicated evaluation system. This study aimed to develop a generic model to evaluate the implementation of the Comprehensive Tobacco Control Law in Iran that was provided based on WHO-FCTC articles. Materials and Methods: Using a grounded theory approach, qualitative data were collected from 265 subjects in individual interviews and focus group discussions with policymakers who designed the legislation, key stakeholders, and members of the target community. In addition, field observations data in supermarkets/shops, restaurants, teahouses and coffee shops were collected. Data were analyzed in two stages through conceptual theoretical coding. Findings: Overall, 617 open codes were extracted from the data into tables; 72 level-3 codes were retained from the level-2 code series. Using a Model Met paradigm, the relationships between the components of each paradigm were depicted graphically. The evaluation model entailed three levels, namely: short-term results, process evaluation and long-term results. Conclusions: Central concept of the process of evaluation is that enforcing the law influences a variety of internal and environmental factors including legislative changes. These factors will be examined during the process evaluation and context evaluation. The current model can be applicable for providing FCTC evaluation tools across other jurisdictions. PMID:23833621

  20. Dynamics of beam halo in mismatched beams

    SciTech Connect

    Wangler, T.P.; Garnett, R.W.; Gray, E.R.; Ryne, R.D.; Wang, T.S.

    1996-09-01

    High-power proton linacs for nuclear materials transmutation and production, and new accelerator-driven neutron spallation sources must be designed to control beam-halo formation, which leads to beam loss. The study of particle-core models is leading to a better understanding of the causes and characteristics of beam halo produced by space-charge forces in rms mismatched beams. Detailed studies of the models have resulted in predictions of the dependence of the maximum amplitude of halo particles on a mismatch parameter and on the space-charge tune-depression ratio. Scaling formulas have been derived which will provide guidance for choosing the aperture radius to contain the halo without loss.

  1. Protein-protein interactions in DNA mismatch repair.

    PubMed

    Friedhoff, Peter; Li, Pingping; Gotthardt, Julia

    2016-02-01

    The principal DNA mismatch repair proteins MutS and MutL are versatile enzymes that couple DNA mismatch or damage recognition to other cellular processes. Besides interaction with their DNA substrates this involves transient interactions with other proteins which is triggered by the DNA mismatch or damage and controlled by conformational changes. Both MutS and MutL proteins have ATPase activity, which adds another level to control their activity and interactions with DNA substrates and other proteins. Here we focus on the protein-protein interactions, protein interaction sites and the different levels of structural knowledge about the protein complexes formed with MutS and MutL during the mismatch repair reaction. PMID:26725162

  2. CONVENTIONAL AND ADVANCED SEWER DESIGN CONCEPTS FOR DUAL PURPOSE FLOOD AND POLLUTION CONTROL. A PRELIMINARY CASE STUDY, ELIZABETH, NEW JERSEY

    EPA Science Inventory

    Alternatives for pollution abatement from combined sewer overflows and stormwater discharges were evaluated. Separate storm and sanitary, conventional combined, and advanced combined systems with varying amounts of in-pipe and/or satellite storage and controlled flow routing were...

  3. 40 CFR 445.12 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... SOURCE CATEGORY RCRA Subtitle C Hazardous Waste Landfill § 445.12 Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR...

  4. 40 CFR 439.13 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30... limitations representing the application of BCT: Limitations for BOD5, TSS and pH are the same as...

  5. 40 CFR 439.33 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30... limitations representing the application of BCT: Limitations for BOD5, TSS and pH are the same as...

  6. 40 CFR 439.23 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... application of the best conventional pollutant control technology (BCT). Except as provided in 40 CFR 125.30... limitations representing the application of BCT: Limitations for BOD5, TSS and pH are the same as...

  7. Project Cerberus: Tobacco Industry Strategy to Create an Alternative to the Framework Convention on Tobacco Control

    PubMed Central

    Mamudu, Hadii M.; Hammond, Ross; Glantz, Stanton A.

    2008-01-01

    Between 1999 and 2001, British American Tobacco, Philip Morris, and Japan Tobacco International executed Project Cerberus to develop a global voluntary regulatory regime as an alternative to the Framework Convention on Tobacco Control (FCTC). They aimed to develop a global voluntary regulatory code to be overseen by an independent audit body and to focus attention on youth smoking prevention. The International Tobacco Products Marketing Standards announced in September 2001, however, did not have the independent audit body. Although the companies did not stop the FCTC, they continue to promote the International Tobacco Products Marketing Standards youth smoking prevention as an alternative to the FCTC. Public health civil society groups should help policymakers and governments understand the importance of not working with the tobacco industry. PMID:18633079

  8. Retracted: Postoperative pain after irrigation with Vibringe versus a conventional needle: a randomized controlled trial.

    PubMed

    Bilgili, D; Yilmaz, S; Dumani, A; Yoldas, O

    2016-08-01

    The following article from International Endodontic Journal, 'Postoperative pain after irrigation with Vibringe versus a conventional needle: a randomized controlled trial' by D. Bilgili, S. Yilmaz, A. Dumani & O. Yoldas, published online on 29 February 2016 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the Journal Editor in Chief, Prof. Paul Dummer, and John Wiley & Sons Ltd. The retraction has been agreed because the corresponding author did not contact the first author who carried out the work before alterations to the article were made prior to submission. This damages the integrity of the work and there are additional concerns over the number of patients and the accuracy of the results and conclusions. PMID:26855328

  9. A Chilling Example? Uruguay, Philip Morris International, and WHO's Framework Convention on Tobacco Control.

    PubMed

    Russell, Andrew; Wainwright, Megan; Mamudu, Hadii

    2015-06-01

    The World Health Organization's Framework Convention on Tobacco Control (FCTC) is the first international public health treaty to address the global spread of tobacco products. Ethnographic research at the fourth meeting of the FCTC's Conference of the Parties in Uruguay highlights the role of the FCTC in recalibrating the relationship between international trade and investment agreements and those of global public health. Specifically, we chart the origins and development of the Punta del Este Declaration, tabled by Uruguay at the conference, to counter a legal request by Philip Morris International, the world's largest tobacco transnational, for arbitration by the International Centre for the Settlement of Investment Disputes over Uruguay's alleged violations of several international trade and investment treaties. We argue that medical anthropologists should give greater consideration to global health governance and diplomacy as a potential counterweight to the 'politics of resignation' associated with corporate capitalism. PMID:25331730

  10. Project Cerberus: tobacco industry strategy to create an alternative to the Framework Convention on Tobacco Control.

    PubMed

    Mamudu, Hadii M; Hammond, Ross; Glantz, Stanton A

    2008-09-01

    Between 1999 and 2001, British American Tobacco, Philip Morris, and Japan Tobacco International executed Project Cerberus to develop a global voluntary regulatory regime as an alternative to the Framework Convention on Tobacco Control (FCTC). They aimed to develop a global voluntary regulatory code to be overseen by an independent audit body and to focus attention on youth smoking prevention. The International Tobacco Products Marketing Standards announced in September 2001, however, did not have the independent audit body. Although the companies did not stop the FCTC, they continue to promote the International Tobacco Products Marketing Standards youth smoking prevention as an alternative to the FCTC. Public health civil society groups should help policymakers and governments understand the importance of not working with the tobacco industry. PMID:18633079

  11. Interpretation of the source-specific substantive control measures of the Minamata Convention on Mercury.

    PubMed

    You, Mingqing

    2015-02-01

    Being persistent, toxic, and bio-accumulative, Mercury (Hg) seriously affects the environment and human health. Due to Hg's attribute of long-range environmental transport across national borders, especially through atmospheric transport, no country can fully protect its environment and human health with its own efforts, without global cooperation. The Minamata Convention on Mercury, which was formally adopted and opened for signature in October 2013, is the only global environmental regime on the control of Hg pollution. Its main substantive control measures are source-specific: its phasing-out, phasing-down, and other main substantive requirements all direct to specific categories of pollution sources through the regulation of specific sectors of the economy and social life. This Convention does not take a national quota approach to quantify the Parties' nationwide total allowable consumption or discharge of Hg or Hg compounds, nor does it quantify their nationwide total reduction requirements. This paper attempts to find the underlying reasons for this source-specific approach and offers two interpretations. One possible interpretation is that Hg might be a non-threshold pollutant, i.e., a pollutant without a risk-free value of concentration. The existence of a reference dose (RfD), reference concentration (RfC), provisional tolerable weekly intake (PTWI), minimal risk level (MRL) or other similar reference values of Hg does not necessarily mean that Hg cannot be regarded as non-threshold because such reference values have scientific uncertainties and may also involve policy considerations. Another interpretation is that Hg lacks a feasibly determinable total allowable quantity. There is evidence that negotiators might have treated Hg as non-threshold, or at least accepted that Hg lacks a feasibly determinable total allowable quantity: (1) The negotiators were informed about the serious situations of the current emissions, releases, and legacy deposition; (2

  12. Hingeless rotor theory and experiment on vibration reduction by periodic variation of conventional controls

    NASA Technical Reports Server (NTRS)

    Sissingh, G. J.; Donham, R. E.

    1974-01-01

    The reduction of the n per rev. pitch-, roll- and vertical vibrations of an n-bladed rotor by n per rev. sinusoidal variations of the collective and cyclic controls is investigated. The numerical results presented refer to a four-bladed, 7.5-foot model and are based on frequency response tests conducted under an Army-sponsored research program. The following subjects are treated: extraction of the rotor transfer functions (.073R hub flapping and model thrust versus servo valve command, amplitude and phase), calculation of servo commands (volts) required to compensate .073R hub flapping (3P and 5P) and model thrust (4P), evaluation of the effect of the vibratory control inputs on blade loads, and theoretical prediction of the root flapbending moments generated by 0 to 5P perturbations of the feathering angle and rotor angle of attack. Five operating conditions are investigated covering advance ratios from approximately 0.2 to 0.85. The feasibility of vibration reduction by periodic variation on conventional controls is evaluated.

  13. [WHO Framework Convention on Tobacco Control (FCTC) Article 11: packaging and labelling of tobacco products].

    PubMed

    Bekki, Kanae; Inaba, Yohei; Kunugita, Naoki

    2015-01-01

    The World Health Organization (WHO) Framework Convention on Tobacco Control (FCTC) requires member countries to implement measures aimed at reducing the demand for tobacco products. FCTC article 11 describes the important forms of health communication and packaging regulations. And this article recommends on large pictorial health warnings and encourages more effective forms of disclosure on constituents and emissions. Furthermore, article 11 recognizes the importance of the package as a promotional vehicle for tobacco companies and requires the removal of potentially misleading packaging information, including the terms "light" and "mild." The Conference of the Parties (COP) adopted guidelines for implementation of article 11 on "Packaging and labelling of Tobacco Products". Some countries, such as Canada, the U.S.A., Australia, EU countries etc. positively promoted tobacco control by implementing countermeasures such as the graphic health warning labels and plain packages. These countermeasures showed the significant effects of decreasing smoking rate and preventing smoking initiation in young people. Furthermore, these warning labels were effective for the literally challenged. However, the Japanese government has not implemented these countermeasures, and only limited texts are shown on Japanese tobacco packaging. Therefore, Japan should emulate approaches taken by other countries, and promote the tobacco control policy in accordance with FCTC. PMID:25744790

  14. Electron energy distribution control by fiat: breaking from the conventional flux ratio scaling rules in etch

    NASA Astrophysics Data System (ADS)

    Ranjan, Alok; Wang, Mingmei; Sherpa, Sonam; Ventzek, Peter

    2015-03-01

    With shrinking critical dimensions, minimizing each of aspect ratio dependent etching (ARDE), bowing, undercut, selectivity, and within die uniformly across a wafer is met by trading off one requirement against another. The problem of trade-offs is especially critical. At the root of the problem is that roles radical flux, ion flux and ion energy play may be both good and bad. Increasing one parameter helps meeting one requirement but hinders meeting the other. Managing process by managing flux ratios and ion energy alone with conventional sources is not adequate because surface chemistry is uncontrollable. At the root of lack of control is that the electron energy distribution function (eedf) has not been controlled. Fortunately the high density surface wave sources control the eedf by fiat. High density surface wave sources are characterized by distinct plasma regions: an active plasma generation region with high electron temperature (Te) and an ionization free but chemistry rich diffusive region (low Te region). Pressure aids is segregating the regions by proving a means for momentum relaxation between the source and downstream region. "Spatial pulsing" allows access to plasma chemistry with reasonably high ion flux, from the active plasma generation region, just above the wafer. Low plasma potential enables precise passivation of surfaces which is critical for atomic layer etch (ALE) or high precision etch where the roles of plasma species can be limited to their purposed roles. High precision etch need not be at the cost of speed and manufacturability. Large ion flux at precisely controlled ion energy with RLSATM realizes fast desorption steps for ALE without compromising process throughput and precision.

  15. Implementation of the Framework Convention on Tobacco Control in Africa: Current Status of Legislation

    PubMed Central

    Tumwine, Jacqueline

    2011-01-01

    Objective To describe, as of July 2011, the status of tobacco control legislation in Africa in three key areas of the Framework Convention on Tobacco Control (FCTC)—(1) Protection from exposure to tobacco smoke, (2) Packaging and labelling of tobacco products, and (3) Tobacco advertising, promotion and sponsorship. Methods Review and analysis of tobacco control legislation in Africa, media reports, journal articles, tobacco industry documents and data published in the 2011 WHO Report on the Global Tobacco Epidemic. Results Modest progress in FCTC implementation in Africa with many countries having legislation or policies on the protection from exposure to tobacco smoke, however, only a handful of countries meet the standards of the FCTC Article 8 and its Guidelines particularly with regards to designated smoking areas. Little progress on packaging and labelling of tobacco products, with few countries having legislation meeting the minimum standards of the FCTC Article 11 and its Guidelines. Mauritius is the only African country with graphic or pictorial health warnings in place and has the largest warning labels in Africa. Slightly better progress in banning tobacco advertising, promotion and sponsorship has been shown by African countries, although the majority of legislation falls short of the standards of the FCTC Article 13 and its Guidelines. Despite their efforts, African countries’ FCTC implementation at national level has not matched the strong regional commitment demonstrated during the FCTC treaty negotiations. Conclusion This study highlights the need for Africa to step up efforts to adopt and implement effective tobacco control legislation that is fully compliant with the FCTC. In order to achieve this, countries should prioritise resources for capacity building for drafting strong FCTC compliant legislation, research to inform policy and boost political will, and countering the tobacco industry which is a major obstacle to FCTC implementation in

  16. [Tobacco smoking and principles of the who framework convention on tobacco control: a review].

    PubMed

    Melkadze, N

    2013-02-01

    The aim of a review is to examine the current state of the relevant publications on tobacco smoking, the Guidelines on Protection from Exposure to Tobacco Smoke, and WHO Framework Convention on Tobacco Control (FCTC), which commits countries to protect the public's health by adopting various measures to reduce demand for tobacco. Georgia ratified the treaty in February 2006. In Georgia the implementation of the WHO FCTC is regulated by the "Law on Tobacco Control" (Law). It went into effect in September 2003. Changes and additions to the Law were approved by the Parliament in December 2008 (N 941 - rs) and in December 2010 (№4059-rs). According to Article 10 of the Law, smoking is prohibited at the educational and childcare institutions, medical and pharmaceutical facilities, at the entire area of petrol, gas and gas-distribution stations, in public transport, indoor areas of work and mass gathering... In spite of the legislation rights of non-smokers are very poorly preserved. With this in mind, the Welfare Foundation, the FCTC and the Tobacco Control Alliance, organized a public discussion on enforcing smoke-free laws in Georgia, in December 2012 at Tbilisi Marriott Courtyard Hotel. In order to make public libraries, educational, cultural institutions «de jure» and «de facto» free from tobacco smoke, the campaign against tobacco, which aims to strengthen implementation of the Tobacco Control Law and Regulation should be held in public libraries - not in the hotels. It is necessary to hang a poster - «Environment free from Smoke» at the entrance to buildings where smoking is prohibited throughout. In Rules and regulations for the use of the library there must be a note: smoking is prohibited in the library. We hope that Georgia in the nearest future will be in the list of countries with smoke-free public and work places. PMID:23482366

  17. Infrequent identity mismatches are frequently undetected

    PubMed Central

    Goldinger, Stephen D.

    2014-01-01

    The ability to quickly and accurately match faces to photographs bears critically on many domains, from controlling purchase of age-restricted goods to law enforcement and airport security. Despite its pervasiveness and importance, research has shown that face matching is surprisingly error prone. The majority of face-matching research is conducted under idealized conditions (e.g., using photographs of individuals taken on the same day) and with equal proportions of match and mismatch trials, a rate that is likely not observed in everyday face matching. In four experiments, we presented observers with photographs of faces taken an average of 1.5 years apart and tested whether face-matching performance is affected by the prevalence of identity mismatches, comparing conditions of low (10 %) and high (50 %) mismatch prevalence. Like the low-prevalence effect in visual search, we observed inflated miss rates under low-prevalence conditions. This effect persisted when participants were allowed to correct their initial responses (Experiment 2), when they had to verify every decision with a certainty judgment (Experiment 3) and when they were permitted “second looks” at face pairs (Experiment 4). These results suggest that, under realistic viewing conditions, the low-prevalence effect in face matching is a large, persistent source of errors. PMID:24500751

  18. Implementation of the WHO Framework Convention on Tobacco Control in mainland China.

    PubMed

    Lv, Jun; Su, Meng; Hong, Zhiheng; Zhang, Ting; Huang, Xuemei; Wang, Bo; Li, Liming

    2011-07-01

    As per China's ratification of the WHO Framework Convention on Tobacco Control (FCTC), it should have implemented effective packaging and labelling measures prior to 9 January 2009 and enacted a comprehensive ban on all tobacco advertising, promotion and sponsorship prior to 9 January 2011. In addition, universal protection against secondhand tobacco smoke should have been implemented before 9 January 2011 by ensuring that all indoor workplaces, all indoor public places, all public transportation and possibly other (outdoor or quasi-outdoor) public places are free of secondhand smoke. The authors conducted a review of various sources of information to determine the current status of FCTC implementation in mainland China. Even though China has made considerable efforts to implement the FCTC, there is still a significant gap between the current state of affairs in China and the requirements of the FCTC. The Chinese tobacco monopoly under which commercial and other vested interests of the tobacco industry are jeopardizing tobacco control efforts is thought to be the most crucial obstacle to the effective implementation of the FCTC across the country. PMID:21493635

  19. Visual mismatch negativity: a predictive coding view.

    PubMed

    Stefanics, Gábor; Kremláček, Jan; Czigler, István

    2014-01-01

    An increasing number of studies investigate the visual mismatch negativity (vMMN) or use the vMMN as a tool to probe various aspects of human cognition. This paper reviews the theoretical underpinnings of vMMN in the light of methodological considerations and provides recommendations for measuring and interpreting the vMMN. The following key issues are discussed from the experimentalist's point of view in a predictive coding framework: (1) experimental protocols and procedures to control "refractoriness" effects; (2) methods to control attention; (3) vMMN and veridical perception. PMID:25278859

  20. Visual mismatch negativity: a predictive coding view

    PubMed Central

    Stefanics, Gábor; Kremláček, Jan; Czigler, István

    2014-01-01

    An increasing number of studies investigate the visual mismatch negativity (vMMN) or use the vMMN as a tool to probe various aspects of human cognition. This paper reviews the theoretical underpinnings of vMMN in the light of methodological considerations and provides recommendations for measuring and interpreting the vMMN. The following key issues are discussed from the experimentalist's point of view in a predictive coding framework: (1) experimental protocols and procedures to control “refractoriness” effects; (2) methods to control attention; (3) vMMN and veridical perception. PMID:25278859

  1. Independent Control of the Magnetization in Ferromagnetic La2/3Sr1/3MnO3/SrTiO3/LaCoO3 Heterostructures Achieved by Epitaxial Lattice Mismatch.

    PubMed

    Rivas-Murias, Beatriz; Lucas, Irene; Jiménez-Cavero, Pilar; Magén, César; Morellón, Luis; Rivadulla, Francisco

    2016-03-01

    We report the effect of interface symmetry-mismatch on the magnetic properties of LaCoO3 (LCO) thin films. Growing epitaxial LCO under tensile strain on top of cubic SrTiO3 (STO) produces a contraction along the c axis and a characteristic ferromagnetic response. However, we report here that ferromagnetism in LCO is completely suppressed when grown on top of a buffer layer of rhombohedral La2/3Sr1/3MnO3 (LSMO), in spite of identical in-plane and out-of-plane lattice deformation. This confirms that it is the lattice symmetry mismatch and not just the total strain, which determines the magnetism of LCO. On the basis of this control over the magnetic properties of LCO, we designed a multilayered structure to achieve independent rotation of the magnetization in ferromagnetic insulating LCO and half-metallic ferromagnet LSMO. This is an important step forward for the design of spin-filtering tunnel barriers based on LCO. PMID:26822394

  2. 78 FR 32458 - Importer of Controlled Substances; Notice of Registration; United States Pharmacopeial Convention

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-30

    ..., 78 FR 17230, United States Pharmacopeial Convention, 12601 Twinbrook Parkway, Rockville, Maryland... powder form from foreign sources for the manufacture of analytical reference standards for sale to...

  3. 77 FR 60144 - Importer of Controlled Substances; Notice of Registration; United States Pharmacopeial Convention

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-02

    ..., 77 FR 40911, United States Pharmacopeial Convention, 12601 Twinbrook Parkway, Rockville, Maryland... (9053) I Heroin (9200) I Morphine-N-oxide (9307) I Amphetamine (1100) II Methamphetamine (1105)...

  4. 40 CFR 442.32 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... pollutant control technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by the application of the best conventional pollutant control technology (BCT). 442.32 Section 442.32 Protection...

  5. Synergistic mercury removal by conventional pollutant control strategies for coal-fired power plants in China.

    PubMed

    Wang, Shuxiao; Zhang, Lei; Wu, Ye; Ancora, Maria Pia; Zhao, Yu; Hao, Jiming

    2010-06-01

    China's 11th 5-yr plan has regulated total sulfur dioxide (SO2) emissions by installing flue gas desulfurization (FGD) devices and shutting down small thermal power units. These control measures will not only significantly reduce the emission of conventional pollutants but also benefit the reduction of mercury emissions from coal-fired power plants. This paper uses the emission factor method to estimate the efficiencies of these measures on mercury emission abatement. From 2005 to 2010, coal consumption in power plants will increase by 59%; however, the mercury emission will only rise from 141 to 155 t, with an increase of 10%. The average emission rate of mercury from coal burning will decrease from 126 mg Hg/t of coal to 87 mg Hg/t of coal. The effects of the three desulfurization measures were assessed and show that wet FGD will play an important role in mercury removal. Mercury emissions in 2015 and 2020 are also projected under different policy scenarios. Under the most probable scenario, the total mercury emission in coal-fired power plants in China will decrease to 130 t by 2020, which will benefit from the rapid installation of fabric filters and selective catalytic reduction. PMID:20564998

  6. Challenges and Potential Solutions for Reducing Climate Control Loads in Conventional and Hybrid Vehicles

    SciTech Connect

    Farrington, R.B., Anderson, R., Blake, D.M., Burch, S.D.; Cuddy, M.R., Keyser, M.A., Rugh, J.P.

    1999-01-01

    The National Renewable Energy Laboratory, a U.S. Department of Energy national laboratory, is collaborating with U.S. automotive manufacturers to develop innovative techniques to reduce national fuel consumption and vehicle tailpipe emissions by reducing vehicle climate control loads. A new U.S. emissions test, the Supplemental Federal Test Procedure (SFTP), will soon begin measuring tailpipe emissions with the air conditioning system operating. Modeled results show that emissions of oxides of nitrogen (NOx) and carbon monoxide (CO) more than double during the air conditioning part of the SFTP. Reducing the transmittance of the glazing can have a greater impact on the cabin soak temperature than ventilating the vehicle during a hot soak. Reducing the amount of outside air can decrease cooling and heating loads but requires that the recirculated air be cleaned. We discuss a photocatalytic oxidation air-cleaning process for removing volatile organic compounds and bioareosols. We conclude with an example of modeling the thermal comfort of the occupants. An auxiliary load increase of only 400 Watts (W) results in a 0.4 km/L (1 mpg) decrease for a conventional 11.9-L/100-km (28-mpg) vehicle. If every vehicle in the United States were to save only 0.4 km/L (1 mpg), $4 billion (U.S. dollars) would be saved annually in gasoline and oil costs. Further information can be found at http://www.ctts.nrel.gov/auxload.html.

  7. Aven-mediated checkpoint kinase control regulates proliferation and resistance to chemotherapy in conventional osteosarcoma.

    PubMed

    Baranski, Zuzanna; Booij, Tijmen H; Cleton-Jansen, Anne-Marie; Price, Leo S; van de Water, Bob; Bovée, Judith V M G; Hogendoorn, Pancras C W; Danen, Erik H J

    2015-07-01

    Conventional high-grade osteosarcoma is the most common primary bone sarcoma, with relatively high incidence in young people. In this study we found that expression of Aven correlates inversely with metastasis-free survival in osteosarcoma patients and is increased in metastases compared to primary tumours. Aven is an adaptor protein that has been implicated in anti-apoptotic signalling and serves as an oncoprotein in acute lymphoblastic leukaemia. In osteosarcoma cells, silencing Aven triggered G2 cell-cycle arrest; Chk1 protein levels were attenuated and ATR-Chk1 DNA damage response signalling in response to chemotherapy was abolished in Aven-depleted osteosarcoma cells, while ATM, Chk2 and p53 activation remained intact. Osteosarcoma is notoriously difficult to treat with standard chemotherapy, and we examined whether pharmacological inhibition of the Aven-controlled ATR-Chk1 response could sensitize osteosarcoma cells to genotoxic compounds. Indeed, pharmacological inhibitors targeting Chk1/Chk2 or those selective for Chk1 synergized with standard chemotherapy in 2D cultures. Likewise, in 3D extracellular matrix-embedded cultures, Chk1 inhibition led to effective sensitization to chemotherapy. Together, these findings implicate Aven in ATR-Chk1 signalling and point towards Chk1 inhibition as a strategy to sensitize human osteosarcomas to chemotherapy. PMID:25757065

  8. Do Iranian tobacco growers support the World Health Organization framework convention on tobacco control?

    PubMed Central

    Sarrafzadegan, Nizal; Rabiei, Katayoun; Mohaseli, Khadijeh Fereydoun; Manzouri, Leila; Roohafza, Hamidreza; Kelishadi, Roya; Abedi, Heidarali; Masooleh, Hasan Azaripour; Alavi, Mousa; Heidari, Gholamreza

    2014-01-01

    Context: Studies on the World Health Organization Frame-work Convention on Tobacco Control (FCTC) are scarce in Iran and the Eastern Mediterranean region (EMR). Aim: This study was conducted in 2007-2008 in Iran to design a practical evaluation model of the implementation of FCTC with the potential to be adopted in the EMR. Given that, the findings of this evaluation can be useful in increasing political and public support for enforcing the implementation of legislations, testing their feasibility, and maintaining sustainability. The viewpoints of tobacco growers as part of stakeholders in this regardwould have an influential role. Settings and Design: This study was a qualitative one to investigate the tobacco growers viewpoints about thestrengths/weaknesses of FCTC implementation in Iran. Materials and Methods: In this study, we conducted semi-structured in-depth individual interviews with 5 tobacco growers. All interviews were carried out with their permissionwere recorded and were assured that their interviews will be kept confidential. All questions were related to different FCTC articles, then written transcripts were prepared and the basic concepts were extrapolated. Statistical Analysis: After transcribing the recorded interviews, we extracted first level codes and main concepts from them. Results: The findings suggested that although tobacco growers agreed with FCTC implementation, however, subjects like the necessity to support tobacco growers and obtaining insurance from the government, the necessity of the enforcement of national tobacco control law and planning to decrease access to tobacco by policy makers were the most key points that tobacco growers pointed to them. Conclusion: Our results showed that tobacco growers agreed with the implementation of FCTC but they worried about their job and the expenses of their daily life. Therefore, it seems that policy makers have to design a plan to support tobacco growers for changing tobacco with a safe

  9. Investment Incentives and the Implementation of the Framework Convention on Tobacco Control: Evidence from Zambia

    PubMed Central

    Drope, Jeffrey; Labonte, Ronald; Zulu, Richard; Goma, Fastone

    2016-01-01

    Purpose Policy misalignment across different sectors of government serves as one of the pivotal barriers to WHO Framework convention on Tobacco Control (FCTC) implementation. This paper examines the logic used by government officials to justify providing investment incentives to increase tobacco processing and manufacturing in the context of FCTC implementation in Zambia. Methods We conducted qualitative semi-structured interviews with key informants from government, civil society and intergovernmental economic organizations (n=23). We supplemented the interview data with an analysis of public documents pertaining to economic development policy in Zambia. Results We found gross misalignments between the policies of the economic sector and efforts to implement the provisions of the FCTC. Our interviews uncovered the rationale used by officials in the economic sector to justify providing economic incentives to bolster tobacco processing and manufacturing in Zambia: 1) tobacco is not consumed by Zambians/tobacco is an export commodity, 2) economic benefits outweigh health costs, and 3) tobacco consumption is a personal choice. Conclusions Much of the struggle Zambia has experienced implementing the FCTC can be attributed to misalignments between the economic and health sectors. Zambia’s development agenda seeks to bolster agricultural processing and manufacturing. Tobacco control proponents must understand and work within this context of economic development in order to foster productive strategies with those working on tobacco supply issues. These findings are broadly applicable to the global analysis on the barriers and facilitators of FCTC implementation. It is important that the Ministry of Health monitors the tobacco policy of other sectors and engages with these sectors to find ways of harmonizing FCTC implementation across sectors. PMID:26135987

  10. Mismatch negativity, social cognition, and functional outcomes in patients after traumatic brain injury

    PubMed Central

    Sun, Hui-yan; Li, Qiang; Chen, Xi-ping; Tao, Lu-yang

    2015-01-01

    Mismatch negativity is generated automatically, and is an early monitoring indicator of neuronal integrity impairment and functional abnormality in patients with brain injury, leading to decline of cognitive function. Antipsychotic medication cannot affect mismatch negativity. The present study aimed to explore the relationships of mismatch negativity with neurocognition, daily life and social functional outcomes in patients after brain injury. Twelve patients with traumatic brain injury and 12 healthy controls were recruited in this study. We examined neurocognition with the Wechsler Adult Intelligence Scale-Revised China, and daily and social functional outcomes with the Activity of Daily Living Scale and Social Disability Screening Schedule, respectively. Mismatch negativity was analyzed from electroencephalogram recording. The results showed that mismatch negativity amplitudes decreased in patients with traumatic brain injury compared with healthy controls. Mismatch negativity amplitude was negatively correlated with measurements of neurocognition and positively correlated with functional outcomes in patients after traumatic brain injury. Further, the most significant positive correlations were found between mismatch negativity in the fronto-central region and measures of functional outcomes. The most significant positive correlations were also found between mismatch negativity at the FCz electrode and daily living function. Mismatch negativity amplitudes were extremely positively associated with Social Disability Screening Schedule scores at the Fz electrode in brain injury patients. These experimental findings suggest that mismatch negativity might efficiently reflect functional outcomes in patients after traumatic brain injury. PMID:26170824

  11. Mismatch negativity, social cognition, and functional outcomes in patients after traumatic brain injury.

    PubMed

    Sun, Hui-Yan; Li, Qiang; Chen, Xi-Ping; Tao, Lu-Yang

    2015-04-01

    Mismatch negativity is generated automatically, and is an early monitoring indicator of neuronal integrity impairment and functional abnormality in patients with brain injury, leading to decline of cognitive function. Antipsychotic medication cannot affect mismatch negativity. The present study aimed to explore the relationships of mismatch negativity with neurocognition, daily life and social functional outcomes in patients after brain injury. Twelve patients with traumatic brain injury and 12 healthy controls were recruited in this study. We examined neurocognition with the Wechsler Adult Intelligence Scale-Revised China, and daily and social functional outcomes with the Activity of Daily Living Scale and Social Disability Screening Schedule, respectively. Mismatch negativity was analyzed from electroencephalogram recording. The results showed that mismatch negativity amplitudes decreased in patients with traumatic brain injury compared with healthy controls. Mismatch negativity amplitude was negatively correlated with measurements of neurocognition and positively correlated with functional outcomes in patients after traumatic brain injury. Further, the most significant positive correlations were found between mismatch negativity in the fronto-central region and measures of functional outcomes. The most significant positive correlations were also found between mismatch negativity at the FCz electrode and daily living function. Mismatch negativity amplitudes were extremely positively associated with Social Disability Screening Schedule scores at the Fz electrode in brain injury patients. These experimental findings suggest that mismatch negativity might efficiently reflect functional outcomes in patients after traumatic brain injury. PMID:26170824

  12. Breaking Free of Control: How Conventional T Cells Overcome Regulatory T Cell Suppression.

    PubMed

    Mercadante, Emily R; Lorenz, Ulrike M

    2016-01-01

    Conventional T (Tcon) cells are crucial in shaping the immune response, whether it is protection against a pathogen, a cytotoxic attack on tumor cells, or an unwanted response to self-antigens in the context of autoimmunity. In each of these immune settings, regulatory T cells (Tregs) can potentially exert control over the Tcon cell response, resulting in either suppression or activation of the Tcon cells. Under physiological conditions, Tcon cells are able to transiently overcome Treg-imposed restraints to mount a protective response against an infectious threat, achieving clonal expansion, differentiation, and effector function. However, evidence has accumulated in recent years to suggest that Tcon cell resistance to Treg-mediated suppression centrally contributes to the pathogenesis of autoimmune disease. Tipping the balance too far in the other direction, cancerous tumors utilize Tregs to establish an overly suppressive microenvironment, preventing antitumor Tcon cell responses. Given the wide-ranging clinical importance of the Tcon/Treg interaction, this review aims to provide a better understanding of what determines whether a Tcon cell is susceptible to Treg-mediated suppression and how perturbations to this finely tuned balance play a role in pathological conditions. Here, we focus in detail on the complex array of factors that confer Tcon cells with resistance to Treg suppression, which we have divided into two categories: (1) extracellular factor-mediated signaling and (2) intracellular signaling molecules. Further, we explore the therapeutic implications of manipulating the phosphatidylinositol-3 kinase (PI3K)/Akt signaling pathway, which is proposed to be the convergence point of signaling pathways that mediate Tcon resistance to suppression. Finally, we address important unresolved questions on the timing and location of acquisition of resistance, and the stability of the "Treg-resistant" phenotype. PMID:27242798

  13. Breaking Free of Control: How Conventional T Cells Overcome Regulatory T Cell Suppression

    PubMed Central

    Mercadante, Emily R.; Lorenz, Ulrike M.

    2016-01-01

    Conventional T (Tcon) cells are crucial in shaping the immune response, whether it is protection against a pathogen, a cytotoxic attack on tumor cells, or an unwanted response to self-antigens in the context of autoimmunity. In each of these immune settings, regulatory T cells (Tregs) can potentially exert control over the Tcon cell response, resulting in either suppression or activation of the Tcon cells. Under physiological conditions, Tcon cells are able to transiently overcome Treg-imposed restraints to mount a protective response against an infectious threat, achieving clonal expansion, differentiation, and effector function. However, evidence has accumulated in recent years to suggest that Tcon cell resistance to Treg-mediated suppression centrally contributes to the pathogenesis of autoimmune disease. Tipping the balance too far in the other direction, cancerous tumors utilize Tregs to establish an overly suppressive microenvironment, preventing antitumor Tcon cell responses. Given the wide-ranging clinical importance of the Tcon/Treg interaction, this review aims to provide a better understanding of what determines whether a Tcon cell is susceptible to Treg-mediated suppression and how perturbations to this finely tuned balance play a role in pathological conditions. Here, we focus in detail on the complex array of factors that confer Tcon cells with resistance to Treg suppression, which we have divided into two categories: (1) extracellular factor-mediated signaling and (2) intracellular signaling molecules. Further, we explore the therapeutic implications of manipulating the phosphatidylinositol-3 kinase (PI3K)/Akt signaling pathway, which is proposed to be the convergence point of signaling pathways that mediate Tcon resistance to suppression. Finally, we address important unresolved questions on the timing and location of acquisition of resistance, and the stability of the “Treg-resistant” phenotype. PMID:27242798

  14. Experimental investigation of piston heat transfer under conventional diesel and reactivity-controlled compression ignition combustion regimes

    SciTech Connect

    Splitter, Derek A; Hendricks, Terry Lee; Ghandhi, Jaal B

    2014-01-01

    The piston of a heavy-duty single-cylinder research engine was instrumented with 11 fast-response surface thermocouples, and a commercial wireless telemetry system was used to transmit the signals from the moving piston. The raw thermocouple data were processed using an inverse heat conduction method that included Tikhonov regularization to recover transient heat flux. By applying symmetry, the data were compiled to provide time-resolved spatial maps of the piston heat flux and surface temperature. A detailed comparison was made between conventional diesel combustion and reactivity-controlled compression ignition combustion operations at matched conditions of load, speed, boost pressure, and combustion phasing. The integrated piston heat transfer was found to be 24% lower, and the mean surface temperature was 25 C lower for reactivity-controlled compression ignition operation as compared to conventional diesel combustion, in spite of the higher peak heat release rate. Lower integrated piston heat transfer for reactivity-controlled compression ignition was found over all the operating conditions tested. The results showed that increasing speed decreased the integrated heat transfer for conventional diesel combustion and reactivity-controlled compression ignition. The effect of the start of injection timing was found to strongly influence conventional diesel combustion heat flux, but had a negligible effect on reactivity-controlled compression ignition heat flux, even in the limit of near top dead center high-reactivity fuel injection timings. These results suggest that the role of the high-reactivity fuel injection does not significantly affect the thermal environment even though it is important for controlling the ignition timing and heat release rate shape. The integrated heat transfer and the dynamic surface heat flux were found to be insensitive to changes in boost pressure for both conventional diesel combustion and reactivity-controlled compression ignition

  15. 78 FR 54913 - Importer of Controlled Substances; Notice of Registration; United States Pharmacopeial Convention

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-06

    ... FR 32457, United States Pharmacopeial Convention, 12601 Twinbrook Parkway, Rockville, Maryland 20852...-oxide (9053) I Difenoxin (9168) I Heroin (9200) I Morphine-N-oxide (9307) I Norlevorphanol (9634)...

  16. Educational Mismatch and Self-Employment

    ERIC Educational Resources Information Center

    Bender, Keith A.; Roche, Kristen

    2013-01-01

    Previous research on educational mismatch concentrates on estimating its labor market consequences but with a focus on wage and salary workers. This paper examines the far less studied influence of mismatch on the self-employed. Using a sample of workers in science and engineering fields, results show larger earnings penalties for mismatch among…

  17. 40 CFR 445.12 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 445.12 Section 445.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  18. 40 CFR 439.23 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 439.23 Section 439.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  19. 40 CFR 437.43 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... provided in 40 CFR 125.30 through 125.32 or 437.40(b), any existing facility subject to this subpart which... application of the best conventional pollutant control technology (BCT). 437.43 Section 437.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  20. 40 CFR 442.42 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 442.42 Section 442.42 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  1. 40 CFR 412.44 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 412.44 Section 412.44 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED...

  2. 40 CFR 412.44 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 412.44 Section 412.44 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CONCENTRATED...

  3. 40 CFR 439.13 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 439.13 Section 439.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  4. 40 CFR 437.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... in 40 CFR 125.30 through 125.32 or 437.20(b), any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 437.22 Section 437.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  5. 40 CFR 437.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... in 40 CFR 125.30 through 125.32 or 437.20(b), any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 437.22 Section 437.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS THE...

  6. 40 CFR 445.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 445.22 Section 445.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  7. 40 CFR 442.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... technology (BCT). Except as provided in 40 CFR 125.30 through 125.32, any existing point source subject to... application of the best conventional pollutant control technology (BCT). 442.22 Section 442.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  8. 40 CFR 437.43 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... provided in 40 CFR 125.30 through 125.32 or 437.40(b), any existing facility subject to this subpart which... application of the best conventional pollutant control technology (BCT). 437.43 Section 437.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  9. 40 CFR 439.23 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 439.23 Section 439.23 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  10. 40 CFR 437.32 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... as provided in 40 CFR 125.30 through 125.32 or § 437.30(b), any existing point source subject to this... application of the best conventional pollutant control technology (BCT). 437.32 Section 437.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  11. 40 CFR 439.43 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 439.43 Section 439.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  12. 40 CFR 437.22 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in 40 CFR 125.30 through 125.32 or 437.20(b), any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 437.22 Section 437.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  13. 40 CFR 439.33 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 439.33 Section 439.33 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  14. 40 CFR 439.13 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must achieve the... application of the best conventional pollutant control technology (BCT). 439.13 Section 439.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS...

  15. 40 CFR 437.32 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... in 40 CFR 125.30 through 125.32 or § 437.30(b), any existing point source subject to this subpart... application of the best conventional pollutant control technology (BCT). 437.32 Section 437.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS THE...

  16. 40 CFR 444.14 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... as provided in 40 CFR 125.30 through 125.32, any existing point source subject to this subpart must... application of the best conventional pollutant control technology (BCT). 444.14 Section 444.14 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  17. 40 CFR 437.32 - Effluent limitations attainable by the application of the best conventional pollutant control...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... as provided in 40 CFR 125.30 through 125.32 or § 437.30(b), any existing point source subject to this... application of the best conventional pollutant control technology (BCT). 437.32 Section 437.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED)...

  18. Pharmacogenetics-Based versus Conventional Dosing of Warfarin: A Meta-Analysis of Randomized Controlled Trials

    PubMed Central

    Shi, Changcheng; Yan, Wei; Wang, Gang; Wang, Fei; Li, Qingyu; Lin, Nengming

    2015-01-01

    Background Recently, using the patient’s genotype to guide warfarin dosing has gained interest; however, whether pharmacogenetics-based dosing (PD) improves clinical outcomes compared to conventional dosing (CD) remains unclear. Thus, we performed a meta-analysis to evaluate these two strategies. Methods The PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure (CNKI), Chinese VIP and Chinese Wan-fang databases were searched. The Cochrane Collaboration’s tool was used to assess the risk of bias in randomized controlled trials (RCTs). The primary outcome was time within the therapeutic range (TTR); the secondary end points were the time to maintenance dose and time to first therapeutic international normalized ratio (INR), an INR greater than 4, adverse events, major bleeding, thromboembolism and death from any cause. Results A total of 11 trials involving 2,678 patients were included in our meta-analysis. The results showed that PD did not improve the TTR compared to CD, although PD significantly shortened the time to maintenance dose (MD = -8.80; 95% CI: -11.99 to -5.60; P<0.00001) and the time to first therapeutic INR (MD = -2.80; 95% CI: -3.45 to -2.15; P<0.00001). Additionally, PD significantly reduced the risk of adverse events (RR = 0.86; 95% CI: 0.75 to 0.99; P = 0.03) and major bleeding (RR = 0.36; 95% CI: 0.15 to 0.89, P = 0.03), although it did not reduce the percentage of INR greater than 4, the risk of thromboembolic events and death from any cause. Subgroup analysis showed that PD resulted in a better improvement in the endpoints of TTR and over-anticoagulation at a fixed initial dosage rather than a non-fixed initial dosage. Conclusions The use of genotype testing in the management of warfarin anticoagulation was associated with significant improvements in INR-related and clinical outcomes. Thus, genotype-based regimens can be considered a reliable and accurate method to determine warfarin dosing and may be preferred over fixed

  19. Comparison between Variable and Conventional Volume-Controlled Ventilation on Cardiorespiratory Parameters in Experimental Emphysema

    PubMed Central

    Henriques, Isabela; Padilha, Gisele A.; Huhle, Robert; Wierzchon, Caio; Miranda, Paulo J. B.; Ramos, Isalira P.; Rocha, Nazareth; Cruz, Fernanda F.; Santos, Raquel S.; de Oliveira, Milena V.; Souza, Sergio A.; Goldenberg, Regina C.; Luiz, Ronir R.; Pelosi, Paolo; de Abreu, Marcelo G.; Silva, Pedro L.; Rocco, Patricia R. M.

    2016-01-01

    Emphysema is characterized by loss of lung tissue elasticity and destruction of structures supporting alveoli and capillaries. The impact of mechanical ventilation strategies on ventilator-induced lung injury (VILI) in emphysema is poorly defined. New ventilator strategies should be developed to minimize VILI in emphysema. The present study was divided into two protocols: (1) characterization of an elastase-induced emphysema model in rats and identification of the time point of greatest cardiorespiratory impairment, defined as a high specific lung elastance associated with large right ventricular end-diastolic area; and (2) comparison between variable (VV) and conventional volume-controlled ventilation (VCV) on lung mechanics and morphometry, biological markers, and cardiac function at that time point. In the first protocol, Wistar rats (n = 62) received saline (SAL) or porcine pancreatic elastase (ELA) intratracheally once weekly for 4 weeks, respectively. Evaluations were performed 1, 3, 5, or 8 weeks after the last intratracheal instillation of saline or elastase. After identifying the time point of greatest cardiorespiratory impairment, an additional 32 Wistar rats were randomized into the SAL and ELA groups and then ventilated with VV or VCV (n = 8/group) [tidal volume (VT) = 6 mL/kg, positive end-expiratory pressure (PEEP) = 3 cmH2O, fraction of inspired oxygen (FiO2) = 0.4] for 2 h. VV was applied on a breath-to-breath basis as a sequence of randomly generated VT values (mean VT = 6 mL/kg), with a 30% coefficient of variation. Non-ventilated (NV) SAL and ELA animals were used for molecular biology analysis. The time point of greatest cardiorespiratory impairment, was observed 5 weeks after the last elastase instillation. At this time point, interleukin (IL)-6, cytokine-induced neutrophil chemoattractant (CINC)-1, amphiregulin, angiopoietin (Ang)-2, and vascular endothelial growth factor (VEGF) mRNA levels were higher in ELA compared to SAL. In ELA animals

  20. Comparison between Variable and Conventional Volume-Controlled Ventilation on Cardiorespiratory Parameters in Experimental Emphysema.

    PubMed

    Henriques, Isabela; Padilha, Gisele A; Huhle, Robert; Wierzchon, Caio; Miranda, Paulo J B; Ramos, Isalira P; Rocha, Nazareth; Cruz, Fernanda F; Santos, Raquel S; de Oliveira, Milena V; Souza, Sergio A; Goldenberg, Regina C; Luiz, Ronir R; Pelosi, Paolo; de Abreu, Marcelo G; Silva, Pedro L; Rocco, Patricia R M

    2016-01-01

    Emphysema is characterized by loss of lung tissue elasticity and destruction of structures supporting alveoli and capillaries. The impact of mechanical ventilation strategies on ventilator-induced lung injury (VILI) in emphysema is poorly defined. New ventilator strategies should be developed to minimize VILI in emphysema. The present study was divided into two protocols: (1) characterization of an elastase-induced emphysema model in rats and identification of the time point of greatest cardiorespiratory impairment, defined as a high specific lung elastance associated with large right ventricular end-diastolic area; and (2) comparison between variable (VV) and conventional volume-controlled ventilation (VCV) on lung mechanics and morphometry, biological markers, and cardiac function at that time point. In the first protocol, Wistar rats (n = 62) received saline (SAL) or porcine pancreatic elastase (ELA) intratracheally once weekly for 4 weeks, respectively. Evaluations were performed 1, 3, 5, or 8 weeks after the last intratracheal instillation of saline or elastase. After identifying the time point of greatest cardiorespiratory impairment, an additional 32 Wistar rats were randomized into the SAL and ELA groups and then ventilated with VV or VCV (n = 8/group) [tidal volume (VT) = 6 mL/kg, positive end-expiratory pressure (PEEP) = 3 cmH2O, fraction of inspired oxygen (FiO2) = 0.4] for 2 h. VV was applied on a breath-to-breath basis as a sequence of randomly generated VT values (mean VT = 6 mL/kg), with a 30% coefficient of variation. Non-ventilated (NV) SAL and ELA animals were used for molecular biology analysis. The time point of greatest cardiorespiratory impairment, was observed 5 weeks after the last elastase instillation. At this time point, interleukin (IL)-6, cytokine-induced neutrophil chemoattractant (CINC)-1, amphiregulin, angiopoietin (Ang)-2, and vascular endothelial growth factor (VEGF) mRNA levels were higher in ELA compared to SAL. In ELA animals

  1. Structural Properties of Mismatched Alloys

    NASA Astrophysics Data System (ADS)

    Mousseau, Normand

    The problem of understanding the local structure of disordered alloys has been around for a long time. In this thesis, I look more specifically at the effect of size-mismatch disorder in binary alloys under many forms: metallic and semiconductor alloys, bulk and surfaces, two and three dimensional systems. I have studied the limitations of a central-force model (CFM) and an embedded-atom potential (EAM) in describing the local structure of binary metallic alloys composed of Ag, Au, Cu, Ni, Pd, or Pt. Although an analytical model developed using the CFM explains qualitatively well the experimental and numerical results, in many cases, it is important to add electronic density effects through a more sophisticated potential like EAM in order to agree quantitatively with experiment. I have also looked at amorphous and crystalline silicon-germanium alloys. It turns out that the effect of size-mismatch is the same on a crystalline and an amorphous lattice. In the latter case, it can be seen as a perturbation of the much larger disorder due to the amorphisation process. However, the analytical predictions differ, for both the crystalline and amorphous alloys, from the experimental results. If one is to believe the data, there is only one possible explanation for this inconsistency: large amounts of hydrogen are present in the samples used for the measurements. Since the data analysis of EXAFS results is not always straightforward, I have proposed some experiments that could shed light on this problem. One of these experiments would be to look at the (111) surface of a Si-Ge alloy with a scanning tunneling microscope. I also present in this thesis the theoretical predictions for the height distribution at the surface as well as some more general structural information about the relaxation in the network as one goes away from the surface. Finally, I have studied the effect of size -mismatch in a purely two dimensional lattice, looking for mismatch-driven phase transitions

  2. Analysis of the computed torque drive method and comparison with conventional position servo for a computer-controlled manipulator

    NASA Technical Reports Server (NTRS)

    Markiewicz, B. R.

    1973-01-01

    A manipulator and its control system (modeled after a Stanford design) is being developed as part of an artificial intelligence project. This development includes an analytical study of the control system software. A comparison is presented of the computed torque method and the conventional position servo. No conclusion is made as to the perference of one system over the other, as it is dependent upon the application and the results of a sampled data analysis.

  3. The structural impact of DNA mismatches.

    PubMed

    Rossetti, Giulia; Dans, Pablo D; Gomez-Pinto, Irene; Ivani, Ivan; Gonzalez, Carlos; Orozco, Modesto

    2015-04-30

    The structure and dynamics of all the transversion and transition mismatches in three different DNA environments have been characterized by molecular dynamics simulations and NMR spectroscopy. We found that the presence of mismatches produced significant local structural alterations, especially in the case of purine transversions. Mismatched pairs often show promiscuous hydrogen bonding patterns, which interchange among each other in the nanosecond time scale. This therefore defines flexible base pairs, where breathing is frequent, and where distortions in helical parameters are strong, resulting in significant alterations in groove dimension. Even if the DNA structure is plastic enough to absorb the structural impact of the mismatch, local structural changes can be propagated far from the mismatch site, following the expected through-backbone and a previously unknown through-space mechanism. The structural changes related to the presence of mismatches help to understand the different susceptibility of mismatches to the action of repairing proteins. PMID:25820425

  4. The structural impact of DNA mismatches

    PubMed Central

    Rossetti, Giulia; Dans, Pablo D.; Gomez-Pinto, Irene; Ivani, Ivan; Gonzalez, Carlos; Orozco, Modesto

    2015-01-01

    The structure and dynamics of all the transversion and transition mismatches in three different DNA environments have been characterized by molecular dynamics simulations and NMR spectroscopy. We found that the presence of mismatches produced significant local structural alterations, especially in the case of purine transversions. Mismatched pairs often show promiscuous hydrogen bonding patterns, which interchange among each other in the nanosecond time scale. This therefore defines flexible base pairs, where breathing is frequent, and where distortions in helical parameters are strong, resulting in significant alterations in groove dimension. Even if the DNA structure is plastic enough to absorb the structural impact of the mismatch, local structural changes can be propagated far from the mismatch site, following the expected through-backbone and a previously unknown through-space mechanism. The structural changes related to the presence of mismatches help to understand the different susceptibility of mismatches to the action of repairing proteins. PMID:25820425

  5. 40 CFR 434.84 - Effluent limitations attainable by application of the best conventional pollutant control...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS COAL MINING POINT SOURCE CATEGORY BPT, BAT, BCT LIMITATIONS AND NEW SOURCE PERFORMANCE STANDARDS Western Alkaline Coal Mining § 434.84 Effluent limitations attainable by application of the best conventional pollutant...

  6. 40 CFR 434.84 - Effluent limitations attainable by application of the best conventional pollutant control...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS COAL MINING POINT... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Effluent limitations attainable by... Mining § 434.84 Effluent limitations attainable by application of the best conventional pollutant...

  7. Chimeric Proteins to Detect DNA Damage and Mismatches

    SciTech Connect

    McCutchen-Maloney, S; Malfatti, M; Robbins, K M

    2002-01-14

    assays in which we looked both at (1) preferential degradation of damaged or mismatched DNA after addition of a chimeric protein, and (2) preferential binding of the chimeric proteins to damaged or mismatched DNA. In our experiments, we used various samples containing damage or mismatches and a control sample containing no damage or mismatches. When a chimeric protein was added to the DNA samples, the damage- or mismatch-recognition portion of the chimeric protein bound the samples containing the DNA damage or mismatch, allowing the nuclease portion to degrade the DNA. Thus, we measured both the preferential binding to the damaged or mismatched DNA and the preferential degradation of these same samples. We showed this in gel-based assays as well as in multiplex flow-cytometry assays. This year, we also focused on a thermophilic protein, MutS, which in nature functions to detect mistakes in DNA replication by its mismatch-detection capability in an organism that lives under extreme thermal conditions (75 C). Because of its thermophilic stability, this protein has great potential as a SNP detection tool. However, the results of our multiplex experiments were unclear-potentially because of interference between samples on different beads. In summary, our results using chimeric proteins and MutS suggest that the proteins and methods developed in this project have application for detecting SNPs and DNA damage as well as for genetic testing.

  8. Opposite Drug Prescription and Cost Trajectories following Integrative and Conventional Care for Pain – A Case-Control Study

    PubMed Central

    Sundberg, Tobias; Petzold, Max; Kohls, Niko; Falkenberg, Torkel

    2014-01-01

    Objectives Pharmacotherapy may have a limited role in long-term pain management. Comparative trajectories of drug prescriptions and costs, two quality-of-care indicators for pain conditions, are largely unknown subsequent to conventional or integrative care (IC) management. The objectives of this study were to compare prescribed defined daily doses (DDD) and cost of first line drugs for pain patients referred to conventional or anthroposophic IC in Stockholm County, Sweden. Methods In this retrospective high quality registry case-control study, IC and conventional care patients were identified through inpatient care registries and matched on pain diagnosis (ICD-10: M79), age, gender and socio-demographics. National drug registry data was used to investigate changes in DDD and costs from 90/180 days before, to 90/180 days after, index visits to IC and conventional care. The primary selected drug category was analgesics, complemented by musculo-skeletal system drugs (e.g. anti-inflammatories, muscle relaxants) and psycholeptics (e.g. hypnotics, sedatives). Results After index care visits, conventional care pain patients (n = 1050) compared to IC patients (n = 213), were prescribed significantly more analgesics. The average (95% CI) group difference was 15.2 (6.0 to 24.3), p = 0.001, DDD/patient after 90 days; and 21.5 (7.4 to 35.6), p = 0.003, DDD/patient after 180 days. The cost of the prescribed and sold analgesics was significantly higher for conventional care after 90 days: euro/patient 10.7 (1.3 to 20.0), p = 0.025. Changes in drug prescription and costs for the other drug categories were not significantly different between groups. Conclusions Drug prescriptions and costs of analgesics increased following conventional care and decreased following IC, indicating potentially fewer adverse drug events and beneficial societal cost savings with IC. PMID:24827981

  9. Mismatch response (MMR) in neonates: Beyond refractoriness.

    PubMed

    Háden, Gábor P; Németh, Renáta; Török, Miklós; Winkler, István

    2016-05-01

    In the adult auditory system, deviant detection and updating the representation of the environment is reflected by the event-related potential (ERP) component termed the mismatch negativity (MMN). MMN is elicited when a rare-pitch deviant stimulus is presented amongst frequent standard pitch stimuli. The same stimuli also elicit a similar discriminative ERP component in sleeping newborn infants (termed the mismatch response: MMR). Both the MMN and the MMR can be confounded by responses generated by differential refractoriness of frequency-selective neural populations. Employing a stimulus paradigm designed to minimize this confounding effect, newborns were presented with sequences of pure tones under two conditions: In the oddball block, rare deviant tones (500Hz; 10%) were delivered amongst frequent standards (700Hz; 90%). In the control block, a comparison tone (500Hz) was presented with the same probability as the deviant (10%) along with the four contextual tones (700Hz, 980Hz, 1372Hz, 1920.8Hz; 22.5% each). The significant difference found between the response elicited by the deviant and the comparison tone showed that the response elicited by the deviant in the oddball sequences cannot be fully explained by frequency-specific refractoriness of the neural generators. This shows that neonates process sounds in a context-dependent manner as well as strengthens the correspondence between the adult MMN and the infant MMR. PMID:26898555

  10. Outline of an on-site inspection regime for conventional arms control in Europe

    SciTech Connect

    Not Available

    1994-10-01

    The complexity of the negotiations on Conventional Forces in Europe (CFE) was emphasized recently by General John R. Galvin, SACEUR, when he stated, {open_quotes}The difficulties of comparing the relative strengths of strategic or intermediate-range nuclear arsenals pale in comparison with the problems of assessing the relative capabilities of opposing conventional forces.{open_quotes} Throughout this process, intensive and rigorous verification measures must be developed and enforced to ensure an acceptable degree of reliability. The eventual agreement will require a complex verification monitoring process covering a vast geographical area. The long-term success of the agreement to a large extent will depend on the level of confidence achieved by the verification process and the effective deployment of technological means will be essential to that process.

  11. Hydrolytic function of Exo1 in mammalian mismatch repair

    PubMed Central

    Shao, Hongbing; Baitinger, Celia; Soderblom, Erik J.; Burdett, Vickers; Modrich, Paul

    2014-01-01

    Genetic and biochemical studies have previously implicated exonuclease 1 (Exo1) in yeast and mammalian mismatch repair, with results suggesting that function of the protein in the reaction depends on both its hydrolytic activity and its ability to interact with other components of the repair system. However, recent analysis of an Exo1-E109K knockin mouse has concluded that Exo1 function in mammalian mismatch repair is restricted to a structural role, a conclusion based on a prior report that N-terminal His-tagged Exo1-E109K is hydrolytically defective. Because Glu-109 is distant from the nuclease hydrolytic center, we have compared the activity of untagged full-length Exo1-E109K with that of wild type Exo1 and the hydrolytically defective active site mutant Exo1-D173A. We show that the activity of Exo1-E109K is comparable to that of wild type enzyme in a conventional exonuclease assay and that in contrast to a D173A active site mutant, Exo1-E109K is fully functional in mismatch-provoked excision and repair. We conclude that the catalytic function of Exo1 is required for its participation in mismatch repair. We also consider the other phenotypes of the Exo1-E109K mouse in the context of Exo1 hydrolytic function. PMID:24829455

  12. Imagery mismatch negativity in musicians.

    PubMed

    Herholz, Sibylle C; Lappe, Claudia; Knief, Arne; Pantev, Christo

    2009-07-01

    The present study investigated musical imagery in musicians and nonmusicians by means of magnetoencephalography (MEG). We used a new paradigm in which subjects had to continue familiar melodies in their mind and then judged if a further presented tone was a correct continuation of the melody. Incorrect tones elicited an imagery mismatch negativity (iMMN) in musicians but not in nonmusicians. This finding suggests that the MMN component can be based on an imagined instead of a sensory memory trace and that imagery of music is modulated by musical expertise. PMID:19673775

  13. Elastic mismatch enhances cell motility

    NASA Astrophysics Data System (ADS)

    Bresler, Yony; Palmieri, Benoit; Grant, Martin

    In recent years, the study of physics phenomena in cancer has drawn considerable attention. In cancer metastasis, a soft cancer cell leaves the tumor, and must pass through the endothelium before reaching the bloodstream. Using a phase-field model we have shown that the elasticity mismatch between cells alone is sufficient to enhance the motility of thesofter cancer cell by means of bursty migration, in agreement with experiment. We will present further characterization of these behaviour, as well as new possible applications for this model.

  14. Costa Rica’s implementation of the Framework Convention on Tobacco Control: Overcoming decades of industry dominance

    PubMed Central

    Crosbie, Eric; Sosa, Patricia; Glantz, Stanton A

    2016-01-01

    Objective To analyze the passage of Costa Rica’s 2012 tobacco control law. Materials and methods Review of legislation, newspaper articles, and key informant interviews. Results Tobacco control advocates, in close collaboration with international health groups, recruited national, regional and international experts to testify in the Legislative Assembly, implemented grassroots advocacy campaigns, and generated media coverage to enact strong legislation in March 2012 consistent with the World Health Organization Framework Convention on Tobacco Control, despite tobacco industry lobbying efforts that for decades blocked effective tobacco control legislation. Conclusion Costa Rica’s experience illustrates how with resources, good strategic planning, aggressive tactics and perseverance tobacco control advocates can overcome tobacco industry opposition in the Legislative Assembly and Executive Branch. This determined approach has positioned Costa Rica to become a regional leader in tobacco control. PMID:26879509

  15. Job Sprawl, Spatial Mismatch, and Black Employment Disadvantage

    ERIC Educational Resources Information Center

    Stoll, Michael A.

    2006-01-01

    This paper examines the relationship between job sprawl and the spatial mismatch between blacks and jobs. Using data from a variety of sources, including the 1990 and 2000 U.S. Census and U.S. Department of Commerce's ZIP Code Business Patterns, I control extensively for metropolitan area characteristics and other factors. In addition, I use…

  16. From public health to international law: possible protocols for inclusion in the Framework Convention on Tobacco Control.

    PubMed Central

    Joossens, L.

    2000-01-01

    Faced with a difficult business environment in the United States and the falling demand for cigarettes in industrialized countries, multinational tobacco companies have been competing fiercely to expand their sales in developing countries. Because of the worldwide threat posed by smoking to health and the emphasis being placed by international tobacco companies on marketing in developing countries, an international regulatory strategy, such as the WHO proposed Framework Convention on Tobacco Control, is needed. This review describes from a public health perspective the possible scope and key considerations of protocols that should be included in the convention. The key international areas that should be considered in tobacco control are: prices, smuggling; tax-free tobacco products; advertising and sponsorship; the Internet; testing methods; package design and labelling; agriculture; and information sharing. PMID:10994267

  17. Multicentre randomised control trial comparing real time teledermatology with conventional outpatient dermatological care: societal cost-benefit analysis

    PubMed Central

    Wootton, R; Bloomer, S E; Corbett, R; Eedy, D J; Hicks, N; Lotery, H E; Mathews, C; Paisley, J; Steele, K; Loane, M A

    2000-01-01

    Objectives Comparison of real time teledermatology with outpatient dermatology in terms of clinical outcomes, cost-benefits, and patient reattendance. Design Randomised controlled trial with a minimum follow up of three months. Setting Four health centres (two urban, two rural) and two regional hospitals. Subjects 204 general practice patients requiring referral to dermatology services; 102 were randomised to teledermatology consultation and 102 to traditional outpatient consultation. Main outcome measures Reported clinical outcome of initial consultation, primary care and outpatient reattendance data, and cost-benefit analysis of both methods of delivering care. Results No major differences were found in the reported clinical outcomes of teledermatology and conventional dermatology. Of patients randomised to teledermatology, 55 (54%) were managed within primary care and 47 (46%) required at least one hospital appointment. Of patients randomised to the conventional hospital outpatient consultation, 46 (45%) required at least one further hospital appointment, 15 (15%) required general practice review, and 40 (39%) no follow up visits. Clinical records showed that 42 (41%) patients seen by teledermatology attended subsequent hospital appointments compared with 41 (40%) patients seen conventionally. The net societal cost of the initial consultation was £132.10 per patient for teledermatology and £48.73 for conventional consultation. Sensitivity analysis revealed that if each health centre had allocated one morning session a week to teledermatology and the average round trip to hospital had been 78 km instead of 26 km, the costs of the two methods of care would have been equal. Conclusions Real time teledermatology was clinically feasible but not cost effective compared with conventional dermatological outpatient care. However, if the equipment were purchased at current prices and the travelling distances greater, teledermatology would be a cost effective alternative

  18. DNA Triplet Repeat Expansion and Mismatch Repair

    PubMed Central

    Iyer, Ravi R.; Pluciennik, Anna; Napierala, Marek; Wells, Robert D.

    2016-01-01

    DNA mismatch repair is a conserved antimutagenic pathway that maintains genomic stability through rectification of DNA replication errors and attenuation of chromosomal rearrangements. Paradoxically, mutagenic action of mismatch repair has been implicated as a cause of triplet repeat expansions that cause neurological diseases such as Huntington disease and myotonic dystrophy. This mutagenic process requires the mismatch recognition factor MutSβ and the MutLα (and/or possibly MutLγ) endonuclease, and is thought to be triggered by the transient formation of unusual DNA structures within the expanded triplet repeat element. This review summarizes the current knowledge of DNA mismatch repair involvement in triplet repeat expansion, which encompasses in vitro biochemical findings, cellular studies, and various in vivo transgenic animal model experiments. We present current mechanistic hypotheses regarding mismatch repair protein function in mediating triplet repeat expansions and discuss potential therapeutic approaches targeting the mismatch repair pathway. PMID:25580529

  19. Development and Clinical Application of a Precise Temperature-Control Device as an Alternate for Conventional Moxibustion Therapy

    PubMed Central

    Takayama, Shin; Takashima, Shigeru; Okajima, Junnosuke; Watanabe, Masashi; Kamiya, Tetsuharu; Seki, Takashi; Yamasaki, Miyako; Yaegashi, Nobuo; Yambe, Tomoyuki; Maruyama, Shigenao

    2012-01-01

    Moxibustion therapy has been used in East Asian medicine for more than a thousand years. However, there are some problems associated with this therapy in clinical practice. These problems include lack of control over the treatment temperature, emission of smoke, and uneven temperature distribution over the treatment region. In order to resolve these problems, we developed a precise temperature-control device for use as an alternate for conventional moxibustion therapy. In this paper, we describe the treatment of a single patient with paralytic ileus that was treated with moxibustion. We also describe an evaluation of temperature distribution on the skin surface after moxibustion therapy, the development of a heat-transfer control device (HTCD), an evaluation of the HTCD, and the clinical effects of treatment using the HTCD. The HTCD we developed can heat the skin of the treatment region uniformly, and its effect may be equivalent to conventional moxibustion, without the emission of smoke and smell. This device can be used to treat ileus, abdominal pain, and coldness of abdomen in place of conventional moxibustion in modern hospitals. PMID:22754583

  20. Helicopter gust alleviation, attitude stabilization, and vibration alleviation using individual-blade-control through a conventional swash plate

    NASA Technical Reports Server (NTRS)

    Ham, N. D.

    1985-01-01

    The novel active control system presented for helicopter rotor aerodynamic and aeroelastic problems involves the individual control of each blade in the rotating frame over a wide range of frequencies (up to the sixth harmonic of rotor speed). This Individual Blade Control (IBC) system controls blade pitch by means of broadband electrohydraulic actuators attached to the swash plate (in the case of three blades) or individually to each blade, using acceleratometer signals to furnish control commands to the actuators. Attention is given to IBC's application to blade lag, flapping, and bending dynamics. It is shown that gust alleviation, attitude stabilization, vibration alleviation, and air/ground resonance suppression, are all achievable with a conventional helicopter swash plate.

  1. Control of Structure in Conventional Friction Stir Welds through a Kinematic Theory of Metal Flow

    NASA Technical Reports Server (NTRS)

    Rubisoff, H.A.; Schneider, J.A.; Nunes, A.C.

    2009-01-01

    In friction stir welding (FSW), a rotating pin is translated along a weld seam so as to stir the sides of the seam together. Metal is prevented from flowing up the pin, which would result in plowing/cutting instead of welding, by a shoulder on the pin. In conventional FSW, the weld metal rests on an "anvil", which supports the heavy "plunge" load on the tool. In this study, both embedded tungsten wires along and copper plating on the faying surfaces were used to trace the flow of AA2219 weld metal around the C-FSW tool. The effect of tool rotational speed, travel speed, plunge load, and pin thread pitch on the resulting weld metal flow was evaluated. Plan, longitudinal, and transverse section x-ray radiographs were examined to trace the metal flow paths. The results are interpreted in terms of a kinematic theory of metal flow in FSW.

  2. Verifying the biological weapons convention: The role of technology in biological arms control

    SciTech Connect

    Schiller, M.J.

    1998-12-01

    The 1972 Biological Weapons Convention prohibits the possession and use of biological agents in war, but does not contain provisions for treaty enforcement. The Protocol to the BWC, an effort to improve enforcement of the treaty, has defined specific mechanisms for verification. This thesis examines the potential for technology to support treaty verification. First, the history of the treaty and efforts to improve it are discussed. Second, the requirements for technical support of verification are examined, and criteria are developed to evaluate potential detection systems. Then, technologies for detection and identification are illustrated, including an evaluation of currently available systems. A procedure for the implementation and support of verification technology is provided. This approach demonstrates a specific process of employing technology to enhance deterrence and verify of compliance with the BWC.

  3. EFFECTS OF ORGANIC AND CONVENTIONAL PRACTICES ON WEED CONTROL IN A PERENNIAL CROPPING SYSTEM

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vineyard weed communities were examined under the influence of an organic weed control practice, soil cultivation with a Clemens cultivator, and applications of the herbicide, glyphosate. Experimental treatments (winter-spring glyphosate, spring cultivation, fall-spring cultivation, fall cultivatio...

  4. Veganism Is a Viable Alternative to Conventional Diet Therapy for Improving Blood Lipids and Glycemic Control.

    PubMed

    Trepanowski, John F; Varady, Krista A

    2015-01-01

    The American Diabetes Association (ADA) and the National Cholesterol Education Program (NCEP) have each outlined a set of dietary recommendations aimed at improving glycemic control and blood lipids, respectively. However, traditional vegan diets (low-fat diets that proscribe animal product consumption) are also effective at improving glycemic control, and dietary portfolios (vegan diets that contain prescribed amounts of plant sterols, viscous fibers, soy protein, and nuts) are also effective at improving blood lipids. The purpose of this review was to compare the effects of traditional vegan diets and dietary portfolios with ADA and NCEP diets on body weight, blood lipids, blood pressure, and glycemic control. The main findings are that traditional vegan diets appear to improve glycemic control better than ADA diets in individuals with type 2 diabetes mellitus (T2DM), while dietary portfolios have been consistently shown to improve blood lipids better than NCEP diets in hypercholesterolemic individuals. PMID:24922183

  5. Mismatch Negativity: Translating the Potential

    PubMed Central

    Todd, Juanita; Harms, Lauren; Schall, Ulrich; Michie, Patricia T.

    2013-01-01

    The mismatch negativity (MMN) component of the auditory event-related potential has become a valuable tool in cognitive neuroscience. Its reduced size in persons with schizophrenia is of unknown origin but theories proposed include links to problems in experience-dependent plasticity reliant on N-methyl-d-aspartate glutamate receptors. In this review we address the utility of this tool in revealing the nature and time course of problems in perceptual inference in this illness together with its potential for use in translational research testing animal models of schizophrenia-related phenotypes. Specifically, we review the reasons for interest in MMN in schizophrenia, issues pertaining to the measurement of MMN, its use as a vulnerability index for the development of schizophrenia, the pharmacological sensitivity of MMN and the progress in developing animal models of MMN. Within this process we highlight the challenges posed by knowledge gaps pertaining to the tool and the pharmacology of the underlying system. PMID:24391602

  6. Numerical simulations of flow fields through conventionally controlled wind turbines & wind farms

    NASA Astrophysics Data System (ADS)

    Emre Yilmaz, Ali; Meyers, Johan

    2014-06-01

    In the current study, an Actuator-Line Model (ALM) is implemented in our in-house pseudo-spectral LES solver SP-WIND, including a turbine controller. Below rated wind speed, turbines are controlled by a standard-torque-controller aiming at maximum power extraction from the wind. Above rated wind speed, the extracted power is limited by a blade pitch controller which is based on a proportional-integral type control algorithm. This model is used to perform a series of single turbine and wind farm simulations using the NREL 5MW turbine. First of all, we focus on below-rated wind speed, and investigate the effect of the farm layout on the controller calibration curves. These calibration curves are expressed in terms of nondimensional torque and rotational speed, using the mean turbine-disk velocity as reference. We show that this normalization leads to calibration curves that are independent of wind speed, but the calibration curves do depend on the farm layout, in particular for tightly spaced farms. Compared to turbines in a lone-standing set-up, turbines in a farm experience a different wind distribution over the rotor due to the farm boundary-layer interaction. We demonstrate this for fully developed wind-farm boundary layers with aligned turbine arrangements at different spacings (5D, 7D, 9D). Further we also compare calibration curves obtained from full farm simulations with calibration curves that can be obtained at a much lower cost using a minimal flow unit.

  7. Composition of transgenic soybean seeds with higher γ-linolenic acid content is equivalent to that of conventional control.

    PubMed

    Qin, Fengyun; Kang, Linzhi; Guo, Liqiong; Lin, Junfang; Song, Jingshen; Zhao, Yinhua

    2012-03-01

    γ-Linolenic acid (GLA) has been used as a general nutraceutical for pharmacologic applications, particularly in the treatment of skin conditions such as eczema. Four transgenic soybean lines that produce GLA at high yields (4.21% of total fatty acids, up to 1002-fold) were generated through the stable insertion of the Delta-6-fatty acid desaturase gene isolated from Borago officinalis into the genome of a conventional soybean cultivar. As part of the safety assessment of genetically engineered crops, the transgenic soybean seeds were compared with their parental soybean seeds (nontransgenic) by applying the principle of substantial equivalence. Compositional analyses were conducted by measuring the fatty acids, proximate analysis (moisture, crude protein, crude fat, carbohydrates, TDF, and ash contents), amino acids, lectins, and trypsin inhibitor activity. The present results showed that the specific transgenic cultivar studied was similar to the conventional control. PMID:22324875

  8. Hingeless rotor theory and experiment on vibration reduction by periodic variation of conventional controls

    NASA Technical Reports Server (NTRS)

    Sissingh, G. J.; Donham, R. E.

    1974-01-01

    A preliminary evaluation is made of the concept of vibration reduction by properly selected oscillatory collective and cyclic control applications. The investigations are based on experimental frequency response data covering advance ratios from approximately 0.2 to 0.85. Because there was no instrumentation for the measurement of the pitch and roll vibrations, these values were obtained by properly adding up the flap-bending moments at 3.3 in. Any other quantity representing pitch/roll vibrations can be compensated for in the same fashion. The calculated control inputs required for vibration reduction stay within acceptable limits. For four of the five conditions tested they are smaller than the values used for the frequency response tests. As to be expected, the compensating controls greatly affect the blade loads, i.e., torsion, flap- and chordwise bending.

  9. Building capacity for implementation of the framework convention for tobacco control in Vietnam: lessons for developing countries

    PubMed Central

    Stillman, Frances A.; David, Annette M.; Kibria, Naseeb; Phan, Hai Thi

    2014-01-01

    Effective implementation of the WHO international Framework Convention on Tobacco Control (FCTC) is the key to controlling the tobacco epidemic. Within countries, strong national tobacco control capacity is the primary determinant for successful implementation of the FCTC. This case study of tobacco control policy describes the experience of building national tobacco control capacity in Vietnam under the Reduce Smoking in Vietnam Partnership project within a national capacity-building framework. In the Vietnam experience, four components of tobacco control capacity emerged as especially important to achieve ‘quality’ outputs and measurable outcomes at the implementation level: (i) organizational structure/infrastructure; (ii) leadership and expertise; (iii) partnerships and networks and (iv) data and evidence from research. The experience gained in this project helps in adapting our tobacco control capacity-building model, and the lessons that emerged from this country case study can provide guidance to global funders, tobacco control technical assistance providers and nations as governments endeavor to meet their commitment to the FCTC. PMID:23411160

  10. Spatial Mismatch: A Third Generation Survey.

    ERIC Educational Resources Information Center

    Eagan, J. Vincent

    1999-01-01

    The spatial mismatch argument hypothesizes that racial discrimination in the housing market, together with the suburbanization of low skilled jobs, contributes significantly to the high unemployment and/or low wages of inner city minority workers. Surveys recent spatial mismatch literature and discusses policy alternatives, focusing on areas…

  11. WEED COMMUNITY COMPOSTION IN RESPONSE TO ORGANIC AND CONVENTIONAL WEED CONTROL PRACTICES IN A CALIFORNIA VINEYARD

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Integrated weed management (IWM) employs multiple tactics to control weed infestations, and can be useful in reducing problematic weeds. IWM in California vineyards typically involves the integration of post-emergence herbicides and pre-emergence herbicides, with less emphasis on incorporation of no...

  12. Cost Comparison of Conventional Gray Combined Sewer Overflow Control Infrastructure versus a Green/Gray Combination

    EPA Science Inventory

    This paper outlines a life-cycle cost analysis comparing a green (rain gardens) and gray (tunnels) infrastructure combination to a gray-only option to control combined sewer overflow in the Turkey Creek Combined Sewer Overflow Basin, in Kansas City, MO. The plan area of this Bas...

  13. PILOT-SCALE ASSESSMENT OF CONVENTIONAL PARTICULATE CONTROL TECHNOLOGY FOR PRESSURIZED FLUIDIZED-BED COMBUSTION EMISSIONS

    EPA Science Inventory

    The report gives results of an evaluation of electrostatic precipitator (ESP) and fabric filter particulate control technology for the EPA/Exxon pressurized fluidized-bed combustion (PFBC) Miniplant in Linden, NJ. EPA's mobile ESP and fabric filter pilot facilities were slipstrea...

  14. Designing and evaluating conventional arms control measures: The case of the Korean peninsula

    SciTech Connect

    Han Yongsup.

    1991-01-01

    As a method of designing and evaluating arms control measures, this study takes a combined approach of qualitative analysis and military simulation. Three qualitative criteria derived from the case studies on the Korean and European arms control are used to examine whether these measures are legally binding, verifiable, and negotiable. One quantifiable criterion is used to test against the base case scenario (a one-day surprise attach by North Korea) whether arms control measures will stabilize or destabilize the military status quo on the Peninsula in terms of North Korea's extent of penetration in the actual war situations. Four alternative measures are derived from the analysis: (1) Confidence and Security Building Measures (CSBMs); (2) Establishment of Asymmetric Non-Deployment Zone (NDZ); (3) Reduction of the joint US-South Korean Team Spirit' exercises and North Korea's Forward Deployed Forces; and, (4) Reduction of South Korean and US forces and North Korean Forces. Findings indicate that establishment of the NDZ and a North Korean unilateral reduction are estimated to best achieve the goal of South Korean arms control.

  15. Translating the WHO Framework Convention on Tobacco Control (FCTC): can we use tobacco control as a model for other non-communicable disease control?

    PubMed

    Lien, G; DeLand, K

    2011-12-01

    Tobacco use is the single most preventable cause of death in the world today. Unchecked, tobacco-related deaths will increase to more than eight million per year by 2030. Galvanized by the seriousness of the threat, the Member States of the World Health Organization (WHO) negotiated the WHO Framework Convention on Tobacco Control (WHO FCTC), which entered into force in 2005. The treaty has enjoyed tremendous global success, with more than 170 Parties, and is often called the most powerful tool in the fight against tobacco-related morbidity and mortality. As the world undergoes the long-predicted transition from communicable to noncommunicable diseases (NCDs) posing the greater health burden, seminal ideas, processes, and outcomes like the WHO FCTC can be used to inform decision-making and policy-making. To help begin such knowledge transfer, this paper first examines how tobacco control evolved to become a reasonable, politically feasible topic for treating in the highly globalized context of public health and NCDs. Next, some of the key achievements and challenges that have occurred over the past six years of WHO FCTC implementation are discussed. Finally, a consideration of how some of the successes and lessons learned in tobacco control appear in other NCD contexts is presented. PMID:22041720

  16. Nonlinear power flow control applications to conventional generator swing equations subject to variable generation.

    SciTech Connect

    Robinett, Rush D., III; Wilson, David Gerald

    2010-05-01

    In this paper, the swing equations for renewable generators are formulated as a natural Hamiltonian system with externally applied non-conservative forces. A two-step process referred to as Hamiltonian Surface Shaping and Power Flow Control (HSSPFC) is used to analyze and design feedback controllers for the renewable generator system. This formulation extends previous results on the analytical verification of the Potential Energy Boundary Surface (PEBS) method to nonlinear control analysis and design and justifies the decomposition of the system into conservative and non-conservative systems to enable a two-step, serial analysis and design procedure. In particular, this approach extends the work done by developing a formulation which applies to a larger set of Hamiltonian Systems that has Nearly Hamiltonian Systems as a subset. The results of this research include the determination of the required performance of a proposed Flexible AC Transmission System (FACTS)/storage device to enable the maximum power output of a wind turbine while meeting the power system constraints on frequency and phase. The FACTS/storage device is required to operate as both a generator and load (energy storage) on the power system in this design. The Second Law of Thermodynamics is applied to the power flow equations to determine the stability boundaries (limit cycles) of the renewable generator system and enable design of feedback controllers that meet stability requirements while maximizing the power generation and flow to the load. Necessary and sufficient conditions for stability of renewable generators systems are determined based on the concepts of Hamiltonian systems, power flow, exergy (the maximum work that can be extracted from an energy flow) rate, and entropy rate.

  17. Linking Global Youth Tobacco Survey (GYTS) data to the WHO framework convention on tobacco control: the case for India.

    PubMed

    Sinha, Dhirendra Narain; Reddy, K Srinath; Rahman, Khalilur; Warren, Charles W; Jones, Nathan R; Asma, Samira

    2006-01-01

    India ratified the WHO Framework Convention on Tobacco Control (WHO FCTC) on February 27, 2005. The WHO FCTC is the world's first public health treaty that aims to promote and protect public health and reduce the devastating health and economic impacts of tobacco. Post ratification, each member state as part of general obligation has agreed to develop, implement, periodically update and review comprehensive multisectoral national tobacco control strategies, plans and programmes in accordance with this Convention and the protocols to which it is a Party. The Global Youth Tobacco Survey (GYTS) was developed to track tobacco use among young people across countries and the GYTS surveillance system intends to enhance the capacity of countries to design, implement, and evaluate tobacco control and prevention programs. The South-East Asia Region of WHO has developed the "Regional Strategy for Utilization of the GYTS" to meet this need for countries in the Region. In 2003, India has passed its national tobacco control legislation (India Tobacco Control Act [ITCA]), which includes provisions designed to reduce tobacco consumption and protect citizens from exposure to second hand smoke. Data in the GYTS (India) report can be used as a baseline measure for future evaluation of the tobacco control programs implemented by the Ministry of Health and Family Welfare, Government of India. India has to upscale some provisions of its National Law to accommodate all of the requirements of FCTC. Using determinants measured by GYTS in India, the government can monitor the impact of enforcing various provisions of the ITCA and the progress made in achieving the goals of the WHO FCTC and the Regional Strategies. Effective enforcement of the provisions of ITCA will show in the receding numbers of tobacco use prevalence figures and reduction in the expenditures associated with tobacco use in India. PMID:17191409

  18. Metamer mismatching in practice versus theory.

    PubMed

    Zhang, Xiandou; Funt, Brian; Mirzaei, Hamidreza

    2016-03-01

    Metamer mismatching (the phenomenon that two objects matching in color under one illuminant may not match under a different illuminant) potentially has important consequences for color perception. Logvinenko et al. [PLoS ONE10, e0135029 (2015)] show that in theory the extent of metamer mismatching can be very significant. This paper examines metamer mismatching in practice by computing the volumes of the empirical metamer mismatch bodies and comparing them to the volumes of the theoretical mismatch bodies. A set of more than 25 million unique reflectance spectra is assembled using datasets from several sources. For a given color signal (e.g., CIE XYZ) recorded under a given first illuminant, its empirical metamer mismatch body for a change to a second illuminant is computed as follows: the reflectances having the same color signal when lit by the first illuminant (i.e., reflect metameric light) are computationally relit by the second illuminant, and the convex hull of the resulting color signals then defines the empirical metamer mismatch body. The volume of these bodies is shown to vary systematically with Munsell value and chroma. The empirical mismatch bodies are compared to the theoretical mismatch bodies computed using the algorithm of Logvinenko et al. [IEEE Trans. Image Process.23, 34 (2014)]. There are three key findings: (1) the empirical bodies are found to be substantially smaller than the theoretical ones; (2) the sizes of both the empirical and theoretical bodies show a systematic variation with Munsell value and chroma; and (3) applied to the problem of color-signal prediction, the centroid of the empirical metamer mismatch body is shown to be a better predictor of what a given color signal might become under a specified illuminant than state-of-the-art methods. PMID:26974929

  19. Indicators Developed to Evaluate the International Framework Convention on Tobacco Control in Iran; A Grounded Theory Study

    PubMed Central

    Sarrafzadegan, Nizal; Rabiei, Katayoun; Abedi, Heidarali; Kelishadi, Roya; Fereydoun Mohaseli, Khadijeh; Alavi, Mousa; Roohafza, Hamidreza

    2014-01-01

    This study aimed to develop indicators for evaluating the implementation of The Framework Convention on Tobacco Control (FCTC) in Iran. We used the “grounded theory” framework. Totally, 265 policy-makers, stakeholders, and community members were recruited by purposeful sampling in 2008. After analyzing the gathered data, 251 indicators, including 82 indicators as “applied indicators”, were derived from second-level codes for three groups. A suitable evaluation questionnaire can be designed based on the extracted indicators for policy makers, stakeholders, and the community to follow the implementation of the FCTC in Iran. PMID:24753645

  20. Non-conventional methods for the control of post-harvest pear diseases.

    PubMed

    Mari, M; Bertolini, P; Pratella, G C

    2003-01-01

    Pears are highly perishable products, especially during the post-harvest phase, when considerable losses can occur. Among the fungal diseases, blue mold caused by Penicillium expansum, grey mould caused by Botrytis cinerea, Mucor rot caused by Mucor piriformis are common on pear fruits. Other (weak) pathogens like Phialophora malorum, Alternaria spp., and Cladosporium herbarum tend to infect wounds and senescent fruits. A post-harvest fungicide treatment can reduce decay but effectiveness decreases with the appearance of resistant strains. There is a clear need to develop new and alternative methods of controlling post-harvest diseases. The emerging technologies for the control of post-harvest fungal diseases are essentially threefold: application of antagonistic microorganisms, application of natural antimicrobial substances and application of sanitizing products. Two biological control products, Aspire (Candida oleophila I-182) (Ecogen, Langhorne, PA, USA) and Bio-Save 110 (Pseudomonas syringae) (EcoScience, Worcester, MA, USA; formerly Bio-Save 11) are currently registered in the USA for post-harvest application to pears. Other potential biocontrol agents have been isolated from fruit and shown to suppress post-harvest decay in pear. It is important that evaluation of these microorganisms be carried out in a product formulation because the formulation may improve or diminish antagonistic efficacy depending on the concentration of chemical product and the duration of exposure to the treatment. Plants produce a large number of secondary metabolites with antimicrobial effects on post-harvest pathogens. Detailed studies have been conducted on aromatic compounds, essential oils, volatile substances and isothiocyanates, with encouraging results. In particular, allyl-isothiocyanate used as a volatile substance, controls blue mould in 'Conference' and 'Kaiser' pear inoculated with a thiabendazole-resistant strain. Sanitizing products such as chlorine dioxide, peracetic

  1. Delayed magnetic mismatch negativity field, but not auditory M100 response, in Specific Language Impairment

    PubMed Central

    Roberts, T.P.L.; Heiken, K.; Kahn, S.Y.; Qasmieh, S.; Blaskey, L.; Solot, C.; Parker, W.A.; Verma, R; Edgar, J.C.

    2012-01-01

    Recent studies show that electrophysiological markers of auditory processing such as the cortical 100ms response (M100) and the mismatch field (MMF), derived from magnetoencephalography (MEG), might be used to identify children with autism spectrum disorders - M100 peak latency - and to stratify children with autism according to the degree of language impairment – mismatch field peak latency. The present study examined the latencies of right superior temporal gyrus M100 and mismatch field in a cohort of children and young adolescents with specific language impairment (n=17), in comparison to age and non-verbal IQ matched typically developing controls (n=21). Neither group showed symptoms associated with autism. Whereas M100 latency (reflecting early auditory processing) did not distinguish controls from children with specific language impairment, the later “change detection” mismatch field response was significantly delayed (by >50ms) in the specific language impairment group. Linear discriminant analysis confirmed the role of mismatch field latency (92%) but not M100 latency (8%) in distinguishing groups. Present results add support to the claim that a delayed M100 is specific to autism spectrum disorders (with relative independence of degree of language impairment) and that a delayed mismatch field reflects an abnormality more generally associated with language impairment, suggesting that mismatch field delay in the present specific language impairment group and previously reported in autistic children with language impairment may be indicative of a common neural system dysfunction. PMID:22551948

  2. Immediate versus conventional loaded single implants in the posterior mandible: a meta-analysis of randomized controlled trials.

    PubMed

    Moraschini, V; Porto Barboza, E

    2016-01-01

    The purpose of this meta-analysis was to compare implant survival, marginal bone loss, and complications between immediate and conventional loading of single implants installed in the posterior mandible. An extensive electronic search was performed of PubMed, Web of Science, and the Cochrane Central Register of Controlled Trials to identify relevant articles published up to January 2015. After the selection process, five studies met the eligibility criteria and were included. The results of the meta-analysis were expressed in terms of the odds ratio (OR) or standardized mean difference (SMD), with a confidence interval (CI) of 95%. Results were pooled according to heterogeneity using the fixed- or random-effects model. There was no statistically significant difference between the two techniques (immediate loading vs. conventional loading) with regard to implant survival (OR 1.71, 95% CI 0.40 to 7.36; P=0.47). There was no statistically significant difference in marginal bone loss (SMD -0.58, 95% CI -1.55 to 0.38; P=0.24). The reported mechanical and biological complications were common to both types of intervention, with the exception of probing depth, which was greater following the immediate loading technique (SMD 0.13, 95% CI -0.19 to 0.44), although this was not statistically significant (P=0.43). PMID:26259980

  3. Biophysical modeling of mismatch repair proteins

    NASA Astrophysics Data System (ADS)

    Salsbury, Freddie

    2009-03-01

    Mismatch repair proteins play a vital role in the bology of cancer due to their dual functions as repair proteins and as sensors of DNA damage. Computational modeling of mismatch repair proteins in conjunction with biological experimentation has demonstrated the role of long-range communication in the functions of these proteins. Furthermore, different conformations have been shown to be associated with different cellular functions, and these differences are being exploited in drug discovery. The latest results in this modeling will be presented.

  4. Pain exposure physical therapy (PEPT) compared to conventional treatment in complex regional pain syndrome type 1: a randomised controlled trial

    PubMed Central

    Barnhoorn, Karlijn J; van de Meent, Henk; van Dongen, Robert T M; Klomp, Frank P; Groenewoud, Hans; Samwel, Han; Nijhuis-van der Sanden, Maria W G; Frölke, Jan Paul M; Staal, J Bart

    2015-01-01

    Objective To compare the effectiveness of pain exposure physical therapy (PEPT) with conventional treatment in patients with complex regional pain syndrome type 1 (CRPS-1) in a randomised controlled trial with a blinded assessor. Setting The study was conducted at a level 1 trauma centre in the Netherlands. Participants 56 adult patients with CRPS-1 participated. Three patients were lost to follow-up. Interventions Patients received either PEPT in a maximum of five treatment sessions, or conventional treatment following the Dutch multidisciplinary guideline. Measurements Outcomes were assessed at baseline and at 3, 6 and 9 months after randomisation. The primary outcome measure was the Impairment level Sum Score—Restricted Version (ISS-RV), consisting of visual analogue scale for pain (VAS-pain), McGill Pain Questionnaire, active range of motion (AROM) and skin temperature. Secondary outcome measures included Pain Disability Index (PDI); muscle strength; Short Form 36 (SF-36); disability of arm, shoulder and hand; Lower Limb Tasks Questionnaire (LLTQ); 10 m walk test; timed up-and-go test (TUG) and EuroQol-5D. Results The intention-to-treat analysis showed a clinically relevant decrease in ISS-RV (6.7 points for PEPT and 6.2 points for conventional treatment), but the between-group difference was not significant (0.96, 95% CI −1.56 to 3.48). Participants allocated to PEPT experienced a greater improvement in AROM (between-group difference 0.51, 95% CI 0.07 to 0.94; p=0.02). The per protocol analysis showed larger and significant between-group effects on ISS-RV, VAS-pain, AROM, PDI, SF-36, LLTQ and TUG. Conclusions We cannot conclude that PEPT is superior to conventional treatment for patients with CRPS-1. Further high-quality research on the effects of PEPT is warranted given the potential effects as indicated by the per protocol analysis. Trial registration numbers NCT00817128 and NTR 2090. PMID:26628523

  5. Monitoring Fine-Grained Sediment in the Colorado River Ecosystem, Arizona - Control Network and Conventional Survey Techniques

    USGS Publications Warehouse

    Hazel, Joseph E., Jr.; Kaplinski, Matt; Parnell, Roderic A.; Kohl, Keith; Schmidt, John C.

    2008-01-01

    In 2002, fine-grained sediment (sand, silt, and clay) monitoring in the Colorado River downstream from Glen Canyon Dam was initiated to survey channel topography at scales previously unobtainable in this canyon setting. This report presents the methods used to establish the high-resolution global positioning system (GPS) control network required for this effort as well as the conventional surveying techniques used in the study. Using simultaneous, dual-frequency GPS vector-based methods, the network points were determined to have positioning accuracies of less than 0.03 meters (m) and ellipsoidal height accuracies of between 0.01 and 0.10 m at a 95-percent degree of confidence. We also assessed network point quality with repeated, electronic (optical) total-station observations at 39 points for a total of 362 measurements; the mean range was 0.022 m in horizontal and 0.13 in vertical at a 95-percent confidence interval. These results indicate that the control network is of sufficient spatial and vertical accuracy for collection of airborne and subaerial remote-sensing technologies and integration of these data in a geographic information system on a repeatable basis without anomalies. The monitoring methods were employed in up to 11 discrete reaches over various time intervals. The reaches varied from 1.3 to 6.4 kilometers in length. Field results from surveys in 2000, 2002, and 2004 are described, during which conventional surveying was used to collect more than 3000 points per day. Ground points were used as checkpoints and to supplement areas just below or above the water surface, where remote-sensing data are not collected or are subject to greater error. An accuracy of +or- 0.05 m was identified as the minimum precision of individual ground points. These results are important for assessing digital elevation model (DEM) quality and identifying detection limits of significant change among surfaces generated from remote-sensing technologies.

  6. Variable versus conventional lung protective mechanical ventilation during open abdominal surgery: study protocol for a randomized controlled trial

    PubMed Central

    2014-01-01

    Background General anesthesia usually requires mechanical ventilation, which is traditionally accomplished with constant tidal volumes in volume- or pressure-controlled modes. Experimental studies suggest that the use of variable tidal volumes (variable ventilation) recruits lung tissue, improves pulmonary function and reduces systemic inflammatory response. However, it is currently not known whether patients undergoing open abdominal surgery might benefit from intraoperative variable ventilation. Methods/Design The PROtective VARiable ventilation trial (‘PROVAR’) is a single center, randomized controlled trial enrolling 50 patients who are planning for open abdominal surgery expected to last longer than 3 hours. PROVAR compares conventional (non-variable) lung protective ventilation (CV) with variable lung protective ventilation (VV) regarding pulmonary function and inflammatory response. The primary endpoint of the study is the forced vital capacity on the first postoperative day. Secondary endpoints include further lung function tests, plasma cytokine levels, spatial distribution of ventilation assessed by means of electrical impedance tomography and postoperative pulmonary complications. Discussion We hypothesize that VV improves lung function and reduces systemic inflammatory response compared to CV in patients receiving mechanical ventilation during general anesthesia for open abdominal surgery longer than 3 hours. PROVAR is the first randomized controlled trial aiming at intra- and postoperative effects of VV on lung function. This study may help to define the role of VV during general anesthesia requiring mechanical ventilation. Trial registration Clinicaltrials.gov NCT01683578 (registered on September 3 3012). PMID:24885921

  7. Mismatched quantum filtering and entropic information

    NASA Astrophysics Data System (ADS)

    Tsang, Mankei

    2014-03-01

    Quantum filtering is a signal processing technique that estimates the posterior state of a quantum system under continuous measurements and has become a standard tool in quantum information processing, with applications in quantum state preparation, quantum metrology, and quantum control. If the filter assumes a wrong model due to assumptions or approximations, however, the estimation accuracy is bound to suffer. In this talk I shall present formulas that relate the error penalty caused by quantum filter mismatch to the relative entropy between the true model and the nominal model, with one formula for Gaussian measurements, such as homodyne detection, and another for Poissonian measurements, such as photon counting. These formulas generalize recent seminal results in classical information theory and provide new operational meanings to relative entropy, mutual information, and channel capacity in the context of quantum experiments. See http://arxiv.org/abs/1310.0291 for details. This work is supported by the Singapore National Research Foundation under NRF Grant No. NRF-NRFF2011-07.

  8. The origins, development, effects, and future of the WHO Framework Convention on Tobacco Control: a personal perspective.

    PubMed

    Yach, Derek

    2014-05-17

    Worldwide, more than 1 billion people use tobacco, resulting in about 6 million deaths per year. The tobacco industry's documented history of subverting control efforts required innovative approaches by WHO--led by Gro Harlem Brundtland--including invocation of its constitutional authority to develop treaties. In 2003, WHO member states adopted the WHO Framework Convention on Tobacco Control (WHO FCTC). In the decade since, 177 countries have ratified and started to implement its full provisions. Success has been tempered by new challenges. Tobacco use has fallen in countries that are members of the Organisation for Economic Co-operation and Development but increased in low-income and middle-income countries, a result in no small part of illicit trade and cheap products from China and other unregulated state monopolies. This review of 50 years of policy development aimed at reducing the burden of disease attributable to tobacco reviews the origins and strategies used in forging the WHO FCTC, from the perspective of one who was there. PMID:24461287

  9. Effect of Conventional and Game-based Teaching on Oral Health Status of Children: A Randomized Controlled Trial

    PubMed Central

    Asokan, Sharath; John, Baby; Gopalan, Thiruvenkadam

    2015-01-01

    ABSTRACT Aim: To compare the effectiveness of conventional and game-based teaching on the level of knowledge and practice regarding oral hygiene among 7 to 10-year-old school children. Materials and methods: A total of 60 children aged 8 to 10 years were randomly divided into two groups: groups A and B. The intervention was started after the pretest evaluation of their knowledge regarding oral health and estimation of Debris Index-simplified (DI-S). Children in group A were given oral health education through flash cards once daily for 7 days. Children in group B were educated through the play-way method, i.e. connect the dots game combined with flash cards. The evaluations, regarding oral hygiene and DI-S were recorded on the 8th day after intervention. A follow-up score was also recorded after 1 and 3 months. Statistical analysis was done using paired t-test and Chi-square test. Results: There was significant increase in oral hygiene scores and decrease in debris scores compared to baseline in both groups at 1 week and 1 month. At 3 months interval, both groups showed a decrease in oral hygiene scores from baseline with group B showing highly significant reduction. The mean increase in knowledge score was also significantly better in group B (p < 0.05). Conclusion: The connect the dots game that includes oral health guidelines including good dental hygiene and dietary habits can thus be an effective intervention aid for teaching the basic oral health concepts among school going children. How to cite this article: Kumar Y, Asokan S, John B, Gopalan T. Effect of Conventional and Game-based Teaching on Oral Health Status of Children: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2015;8(2):123-126. PMID:26379380

  10. Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology.

    PubMed

    Daher, Rana K; Stewart, Gale; Boissinot, Maurice; Boudreau, Dominique K; Bergeron, Michel G

    2015-04-01

    Recombinase polymerase amplification (RPA) technology relies on three major proteins, recombinase proteins, single-strand binding proteins, and polymerases, to specifically amplify nucleic acid sequences in an isothermal format. The performance of RPA with respect to sequence mismatches of closely-related non-target molecules is not well documented and the influence of the number and distribution of mismatches in DNA sequences on RPA amplification reaction is not well understood. We investigated the specificity of RPA by testing closely-related species bearing naturally occurring mismatches for the tuf gene sequence of Pseudomonas aeruginosa and/or Mycobacterium tuberculosis and for the cfb gene sequence of Streptococcus agalactiae. In addition, the impact of the number and distribution of mismatches on RPA efficiency was assessed by synthetically generating 14 types of mismatched forward primers for detecting five bacterial species of high diagnostic relevance such as Clostridium difficile, Staphylococcus aureus, S. agalactiae, P. aeruginosa, and M. tuberculosis as well as Bacillus atropheus subsp. globigii for which we use the spores as internal control in diagnostic assays. A total of 87 mismatched primers were tested in this study. We observed that target specific RPA primers with mismatches (n > 1) at their 3'extrimity hampered RPA reaction. In addition, 3 mismatches covering both extremities and the center of the primer sequence negatively affected RPA yield. We demonstrated that the specificity of RPA was multifactorial. Therefore its application in clinical settings must be selected and validated a priori. We recommend that the selection of a target gene must consider the presence of closely-related non-target genes. It is advisable to choose target regions with a high number of mismatches (≥36%, relative to the size of amplicon) with respect to closely-related species and the best case scenario would be by choosing a unique target gene. PMID:25481659

  11. Functional features of crossmodal mismatch responses.

    PubMed

    Zhao, Chen; Valentini, Elia; Hu, Li

    2015-02-01

    Research on brain mechanisms of deviance detection and sensory memory trace formation, best indexed by the mismatch negativity, mainly relied on the investigation of responses elicited by auditory stimuli. However, comparable less research reported the mismatch negativity elicited by somatosensory stimuli. More importantly, little is known on the functional features of mismatch deviant and standard responses across different sensory modalities. To directly compare different sensory modalities, we adopted a crossmodal roving paradigm and collected event-related potentials elicited by auditory, non-nociceptive somatosensory, and nociceptive trains of stimuli, during Active and Passive attentional conditions. We applied a topographical segmentation analysis to cluster successive scalp topographies with quasi-stable landscape of significant differences to extract crossmodal mismatch responses. We obtained three main findings. First, across different sensory modalities and attentional conditions, the formation of a standard sensory trace became robust mainly after the second stimulus repetition. Second, the neural representation of a modality deviant stimulus was influenced by the preceding sensory modality. Third, the mismatch negativity significantly covaried between Active and Passive attentional conditions within the same sensory modality, but not between different sensory modalities. These findings provide robust evidence that, while different modalities share a similar process of standard trace formation, the process of deviance detection is largely modality dependent. PMID:25398556

  12. Memory-based mismatch response to frequency changes in rats.

    PubMed

    Astikainen, Piia; Stefanics, Gabor; Nokia, Miriam; Lipponen, Arto; Cong, Fengyu; Penttonen, Markku; Ruusuvirta, Timo

    2011-01-01

    Any occasional changes in the acoustic environment are of potential importance for survival. In humans, the preattentive detection of such changes generates the mismatch negativity (MMN) component of event-related brain potentials. MMN is elicited to rare changes ('deviants') in a series of otherwise regularly repeating stimuli ('standards'). Deviant stimuli are detected on the basis of a neural comparison process between the input from the current stimulus and the sensory memory trace of the standard stimuli. It is, however, unclear to what extent animals show a similar comparison process in response to auditory changes. To resolve this issue, epidural potentials were recorded above the primary auditory cortex of urethane-anesthetized rats. In an oddball condition, tone frequency was used to differentiate deviants interspersed randomly among a standard tone. Mismatch responses were observed at 60-100 ms after stimulus onset for frequency increases of 5% and 12.5% but not for similarly descending deviants. The response diminished when the silent inter-stimulus interval was increased from 375 ms to 600 ms for +5% deviants and from 600 ms to 1000 ms for +12.5% deviants. In comparison to the oddball condition the response also diminished in a control condition in which no repetitive standards were presented (equiprobable condition). These findings suggest that the rat mismatch response is similar to the human MMN and indicate that anesthetized rats provide a valuable model for studies of central auditory processing. PMID:21915297

  13. Control of damping-off of organic and conventional cucumber with extracts from a plant-associated bacterium rivals a seed treatment pesticide

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Environmentally friendly control measures are needed for soilborne diseases of crops grown in organic and conventional production systems. We tested ethanol extracts from cultures of Serratia marcescens N4-5 and N2-4, Burkholderia cepacia BC-1 and BC-2, and B. ambifaria BC-F for control of damping-o...

  14. Integrated application of active controls (IAAC) technology to an advanced subsonic transport project. Conventional baseline configuration study

    NASA Technical Reports Server (NTRS)

    1980-01-01

    Characteristics of the U.S. domestic fleet were evaluated to determine the mission characteristics that would have the most impact on U. S. transport fuel use in the future. This resulted in selection of a 197-passenger (plus cargo), about 3710-km (2000 nmi) mission. The existing data base was reviewed and additional analysis was conducted as necessary to complete the technical descriptions. The resulting baseline configuration utilizes a double-lobe, but nearly circular, body with seven-abreast seating. External characteristics feature an 8.71 aspect ratio, 31.5-degree sweep wing, a T-tail empennage, and a dual CF6-6D2, wing-mounted engine arrangement. It provides for 22 LD-2 or 11 LD-3 containers plus bulk cargo in the lower lobe. Passenger/cargo loading, servicing provisions, taxi/takeoff speeds, and field length characteristics are all compatible with accepted airline operations and regulatory provisions. The baseline configuration construction uses conventional aluminum structure except for advanced aluminum alloys and a limited amount of graphite epoxy secondary structure. Modern systems are used, including advanced guidance, navigation, and controls which emphasize application of digital electronics and advanced displays.

  15. [Effects of coated controlled release urea combined with conventional urea on winter wheat growth and soil NO3- -N].

    PubMed

    Yi, Wen-ping; Sun, Zhe; Wu, Liang; Shi, Gui-fang; Zhu, Guo-liang; Li, Ya-xing; Gu, Jia-lin; Xu, Qiu-ming

    2011-03-01

    Field experiments were conducted to study the effects of different dosages coated controlled release urea (PCU60, 60 d release duration) combined with conventional urea (U) used as basal on the winter wheat grain yield, nitrogen (N) recovery rate, and soil NO3- -N content, etc. Five treatments were installed, i.e., U (CK), 10% PCU60+90% U (PU1), 20% PCU60+80% U (PU2), 30% PCU60+70% U (PU3), and 40% PCU60+60% U (PU4). In the meantime, a comparative analysis was also carried out on the PCU60 N release characteristics under field condition and in 25 "C static water. At the same N dosage, all the test indices in treatment PU4 were significantly higher, with the grain yield, N recovery rate, total N accumulation amount, total tiller number and aboveground biomass at ripening stage, and economic benefit increased by 5.6%, 14.6%, 7.2%, 2.6%, 7.5%, and 984.3 yuan x hm(-2), respectively, compared with those in treatment U. The accumulation amount of NO3- -N in 0-100 cm soil layer in all treatments ranged in 39.70-49.93 kg x hm-2, and was the lowest (39.70 kg x hm(-2)) in treatment PU4. The N release pattern of PCU60 under field condition better fitted the N absorption characteristics of winter wheat. PMID:21657025

  16. Novel Noxipoint Therapy versus Conventional Physical Therapy for Chronic Neck and Shoulder Pain: Multicentre Randomised Controlled Trials

    PubMed Central

    Koo, Charles C.; Lin, Ray S.; Wang, Tyng-Guey; Tsauo, Jau-Yih; Yang, Pan-Chyr; Yen, Chen-Tung; Biswal, Sandip

    2015-01-01

    As chronic pain affects 115 million people and costs $600B annually in the US alone, effective noninvasive nonpharmacological remedies are desirable. The purpose of this study was to determine the efficacy and the generalisability of Noxipoint therapy (NT), a novel electrotherapy characterised by site-specific stimulation, intensity-and-submodality-specific settings and a immobilization period, for chronic neck and shoulder pain. Ninety-seven heavily pretreated severe chronic neck/shoulder pain patients were recruited; 34 and 44 patients were randomly allocated to different treatment arms in two patient-and-assessor-blinded, randomised controlled studies. The participants received NT or conventional physical therapy including transcutaneous electrical nerve stimulation (PT-TENS) for three to six 90-minute sessions. In Study One, NT improved chronic pain (−89.6%, Brief Pain Inventory, p < 0.0001, 95% confidence interval), function (+77.4%, range of motion) and quality of life (+88.1%) at follow-up (from 4 weeks to 5 months), whereas PT-TENS resulted in no significant changes in these parameters. Study Two demonstrated similar advantages of NT over PT-TENS and the generalisability of NT. NT-like treatments in a randomised rat study showed a similar reduction in chronic hypersensitivity (−81%, p < 0.01) compared with sham treatments. NT substantially reduces chronic neck and shoulder pain, restores function, and improves quality of life in a sustained manner. PMID:26552835

  17. Higher-Than-Conventional Radiation Doses in Localized Prostate Cancer Treatment: A Meta-analysis of Randomized, Controlled Trials

    SciTech Connect

    Viani, Gustavo Arruda Stefano, Eduardo Jose; Afonso, Sergio Luis

    2009-08-01

    Purpose: To determine in a meta-analysis whether the outcomes in men with localized prostate cancer treated with high-dose radiotherapy (HDRT) are better than those in men treated with conventional-dose radiotherapy (CDRT), by quantifying the effect of the total dose of radiotherapy on biochemical control (BC). Methods and Materials: The MEDLINE, EMBASE, CANCERLIT, and Cochrane Library databases, as well as the proceedings of annual meetings, were systematically searched to identify randomized, controlled studies comparing HDRT with CDRT for localized prostate cancer. To evaluate the dose-response relationship, we conducted a meta-regression analysis of BC ratios by means of weighted linear regression. Results: Seven RCTs with a total patient population of 2812 were identified that met the study criteria. Pooled results from these RCTs showed a significant reduction in the incidence of biochemical failure in those patients with prostate cancer treated with HDRT (p < 0.0001). However, there was no difference in the mortality rate (p = 0.38) and specific prostate cancer mortality rates (p = 0.45) between the groups receiving HDRT and CDRT. However, there were more cases of late Grade >2 gastrointestinal toxicity after HDRT than after CDRT. In the subgroup analysis, patients classified as being at low (p = 0.007), intermediate (p < 0.0001), and high risk (p < 0.0001) of biochemical failure all showed a benefit from HDRT. The meta-regression analysis also detected a linear correlation between the total dose of radiotherapy and biochemical failure (BC = -67.3 + [1.8 x radiotherapy total dose in Gy]; p = 0.04). Conclusions: Our meta-analysis showed that HDRT is superior to CDRT in preventing biochemical failure in low-, intermediate-, and high-risk prostate cancer patients, suggesting that this should be offered as a treatment for all patients, regardless of their risk status.

  18. Research Required for the Effective Implementation of the Framework Convention on Tobacco Control, Articles 9 and 10

    PubMed Central

    2013-01-01

    This paper is part of a series of articles intended to set out the research questions that are relevant to the successful implementation of the various provisions of the Framework Convention on Tobacco Control (FCTC). This paper focuses on issues affecting Articles 9 and 10 of the FCTC. This paper focuses on the research that is most important for most countries, rather than on what is desirable in countries with high levels of research capacity. Articles 9 and 10 of the FCTC address the regulation of contents and emissions of tobacco products and regulation of tobacco product disclosure. Such regulation will be essential if the long-term objective of reducing the danger of tobacco products is to be achieved. There are many components of tobacco and tobacco smoke that are excessively toxic and dangerous to the user. Many of these components are carcinogenic and addictive and can be removed or reduced substantially with current known technology. The fact that these components remain in tobacco and tobacco smoke at levels that are unnecessarily dangerous is precisely the reason why the successful implementation of Articles 9 and 10 of the FCTC is important to tobacco control. This paper discusses the scientific challenges involved in successfully implementing Articles 9 and 10 of the FCTC, which focuses on regulating carcinogens and toxins in tobacco and tobacco smoke, the abuse liability of tobacco products, and the additives and engineering features in tobacco products that make tobacco products appealing to future consumers. The research issues we focus on are those required to support the early stages of regulation. As regulation proceeds, new and more sophisticated research questions will undoubtedly emerge. PMID:23024247

  19. Comparison between Chinese Herbal Medicines and Conventional Therapy in the Treatment of Severe Hand, Foot, and Mouth Disease: A Randomized Controlled Trial

    PubMed Central

    Li, Xiuhui; Zhang, Xi; Ding, Jianbo; Xu, Yi; Wei, Dan; Tian, Yimei; Chen, Wei; Huang, Jihan; Li, Shuangjie

    2014-01-01

    Background. This study was made to evaluate the efficacy of Chinese herbal medicines, Reduning injection, and a traditional Chinese medicine (TCM) granule, in patients with severe hand, foot, and mouth disease (HFMD) by conducting a prospective, controlled, and randomized trial. Methods. 355 severe HFMD patients were randomly assigned to receive conventional therapy alone, Reduning injection plus conventional therapy, or TCM enema plus conventional therapy for 7–10 days. Results. There was no significant difference in the incidence of major complications between the groups. Median time to fever clearance was 20 hours (95% CI: 6.0–25.0) for conventional therapy recipients, 18 hours (95% CI: 4.0–24.0) for Reduning combination-treated patients, and 6 hours (95% CI: 4.0–16.0) for TCM combination-treated patients. Only the difference in time to fever clearance between TCM combination group and conventional group reached statistical significance (P = 0.048). Reduning combination group showed a significant reduction in sedative administration compared with conventional therapy group (P = 0.008). No HFMD-related death and no important adverse events were observed. Conclusions. Reduning injection plus conventional therapy significantly reduced the concomitant use of sedatives, which may help decrease HFMD-related neurologic complications in children. TCM effectively reduced time to fever clearance and may become a complementary therapy for relieving the symptoms of severe HFMD. PMID:24719639

  20. Comparison between Chinese Herbal Medicines and Conventional Therapy in the Treatment of Severe Hand, Foot, and Mouth Disease: A Randomized Controlled Trial.

    PubMed

    Li, Xiuhui; Zhang, Xi; Ding, Jianbo; Xu, Yi; Wei, Dan; Tian, Yimei; Chen, Wei; Huang, Jihan; Wen, Tao; Li, Shuangjie

    2014-01-01

    Background. This study was made to evaluate the efficacy of Chinese herbal medicines, Reduning injection, and a traditional Chinese medicine (TCM) granule, in patients with severe hand, foot, and mouth disease (HFMD) by conducting a prospective, controlled, and randomized trial. Methods. 355 severe HFMD patients were randomly assigned to receive conventional therapy alone, Reduning injection plus conventional therapy, or TCM enema plus conventional therapy for 7-10 days. Results. There was no significant difference in the incidence of major complications between the groups. Median time to fever clearance was 20 hours (95% CI: 6.0-25.0) for conventional therapy recipients, 18 hours (95% CI: 4.0-24.0) for Reduning combination-treated patients, and 6 hours (95% CI: 4.0-16.0) for TCM combination-treated patients. Only the difference in time to fever clearance between TCM combination group and conventional group reached statistical significance (P = 0.048). Reduning combination group showed a significant reduction in sedative administration compared with conventional therapy group (P = 0.008). No HFMD-related death and no important adverse events were observed. Conclusions. Reduning injection plus conventional therapy significantly reduced the concomitant use of sedatives, which may help decrease HFMD-related neurologic complications in children. TCM effectively reduced time to fever clearance and may become a complementary therapy for relieving the symptoms of severe HFMD. PMID:24719639

  1. British American Tobacco on Facebook: undermining article 13 of the global World Health Organization Framework Convention on Tobacco Control

    PubMed Central

    Chapman, Simon

    2010-01-01

    Background The World Health Organization Framework Convention on Tobacco Control (WHO FCTC) bans all forms of tobacco advertising, promotion and sponsorship. The comprehensiveness of this ban has yet to be tested by online social networking media such as Facebook. In this paper, the activities of employees of the transnational tobacco company, British American Tobacco, (BAT) on Facebook and the type of content associated with two globally popular BAT brands (Dunhill and Lucky Strike) are mapped. Methods BAT employees on Facebook were identified and then the term ‘British American Tobacco’ was searched for in the Facebook search engine and results recorded, including titles, descriptions, names and the number of Facebook participants involved for each search result. To further detail any potential promotional activities, a search for two of BAT's global brands, ‘Dunhill’ and ‘Lucky Strike’, was conducted. Results Each of the 3 search terms generated more than 500 items across a variety of Facebook subsections. Discussion Some BAT employees are energetically promoting BAT and BAT brands on Facebook through joining and administrating groups, joining pages as fans and posting photographs of BAT events, products and promotional items. BAT employees undertaking these actions are from countries that have ratified the WHO FCTC, which requires signatories to ban all forms of tobacco advertising, including online and crossborder exposure from countries that are not enforcing advertising restrictions. The results of the present research could be used to test the comprehensiveness of the advertising ban by requesting that governments mandate the removal of this promotional material from Facebook. PMID:20395406

  2. A Prospective Randomized Controlled Study of Endoscopic Sphincterotomy With the Endocut Mode or Conventional Blended Cut Mode

    PubMed Central

    Tanaka, Yoshiki; Tsuchida, Hiroyuki; Mizuide, Masafumi; Yasuoka, Hidetoshi; Ishida, Katsutoshi; Mori, Masatomo; Kusano, Motoyasu; Yamada, Masanobu

    2015-01-01

    Background: Although the potential advantages of the Endocut mode (E-mode) of endoscopic sphincterotomy (EST) over the conventional blended cut mode (C-mode) have been reported, the problems, including the small sample size and retrospective analysis, that occurred in previous studies make it difficult to conclude the advantage of the E-mode regarding the safety and efficacy. We performed a prospective randomized controlled study to compare these modes. Methods: A total of 360 patients with choledocholithiasis or stenosis of the bile duct were randomly assigned to one of the modes. To avoid the technical bias due to multiple operators or institutions, the main operator and the institution were restricted to only one experienced doctor and 3 institutions at his place of employment, respectively. We defined pancreatitis, bleeding, and perforation as complications of EST. Besides, bleeding includes endoscopically evident bleeding that was defined as visible during the procedure of sphincterotomy and temporary slight oozing. Results: The complications occurred in 20 (11.2%) patients from the E-mode group: pancreatitis in 6 (3.4%) and endoscopically evident bleeding in 14 (7.8%). In contrast, the complications occurred in 25 (13.8%) patients from the C-mode group: pancreatitis in 7 (3.9%) and endoscopically evident bleeding in 18 (9.9%), although these findings were not statistically significant. Overall, there were no severe complications. There were no significant differences in completion ratio of EST and the time taken for the sphincterotomy between both groups. Conclusions: The E-mode could not surpass the C-mode in safety and efficacy under the operation by a single endoscopist. PMID:24583745

  3. The role of non-governmental organizations in global health diplomacy: negotiating the Framework Convention on Tobacco Control.

    PubMed

    Lencucha, Raphael; Kothari, Anita; Labonté, Ronald

    2011-09-01

    The Framework Convention on Tobacco Control (FCTC) is an exemplar result of global health diplomacy, based on its global reach (binding on all World Health Organization member nations) and its negotiation process. The FCTC negotiations are one of the first examples of various states and non-state entities coming together to create a legally binding tool to govern global health. They have demonstrated that diplomacy, once consigned to interactions among state officials, has witnessed the dilution of its state-centric origins with the inclusion of non-governmental organizations (NGOs) in the diplomacy process. To engage in the discourse of global health diplomacy, NGO diplomats are immediately presented with two challenges: to convey the interests of larger publics and to contribute to inter-state negotiations in a predominantly state-centric system of governance that are often diluted by pressures from private interests or mercantilist self-interest on the part of the state itself. How do NGOs manage these challenges within the process of global health diplomacy itself? What roles do, and can, they play in achieving new forms of global health diplomacy? This paper addresses these questions through presentation of findings from a study of the roles assumed by one group of non-governmental actors (the Canadian NGOs) in the FCTC negotiations. The findings presented are drawn from a larger grounded theory study. Qualitative data were collected from 34 public documents and 18 in-depth interviews with participants from the Canadian government and Canadian NGOs. This analysis yielded five key activities or roles of the Canadian NGOs during the negotiation of the FCTC: monitoring, lobbying, brokering knowledge, offering technical expertise and fostering inclusion. This discussion begins to address one of the key goals of global health diplomacy, namely 'the challenges facing health diplomacy and how they have been addressed by different groups and at different levels of

  4. Finding needles in haystacks: identity mismatch frequency and facial identity verification.

    PubMed

    Bindemann, Markus; Avetisyan, Meri; Blackwell, Kristy-Ann

    2010-12-01

    Accurate person identification is central to all security, police, and judicial systems. A commonplace method to achieve this is to compare a photo-ID and the face of its purported owner. The critical aspect of this task is to spot cases in which these two instances of a face do not match. Studies of person identification show that these instances often go undetected when mismatches occur regularly in an experiment, but this differs from everyday operations in which identity mismatches are rare. The current study therefore examined whether infrequent identity mismatches are more likely to go undetected by observers. In Experiments 1 and 2, identity mismatches were detected equally under low (2%) and high (50%) mismatch prevalence. This pattern persisted when viewing conditions were optimized for person identification in Experiment 3, by using a card-sorting task in which all face identities could be viewed repeatedly, and also under increased task difficulty, by constraining viewing conditions temporally in Experiment 4. These results imply that the infrequent occurrence of identity mismatches in security settings such as passport control does not impair an observer's ability to detect these important events. PMID:21198254

  5. HLA mismatching as a strategy to reduce relapse after alternative donor transplantation.

    PubMed

    Fleischhauer, Katharina; Beelen, Dietrich W

    2016-04-01

    Human leukocyte antigen (HLA) mismatches are targets of alloreactive T cells, mediators of graft-versus-leukemia (GvL) and graft-versus-host disease (GvHD) after alternative donor transplantation. Exploitation of HLA mismatching in order to reduce relapse is hampered by necessary interventions aimed at controlling GvHD on the one hand, and by the possibility of immune escape through selective loss of mismatched HLA in relapsing leukemia on the other. Retrospective studies reporting the impact of HLA mismatches on post-transplant relapse need to be interpreted with caution, due to many confounding factors, including disease and use of T-cell depletion, and to be constantly updated to the rapidly changing clinical protocols. Current evidence suggests similar relapse rates for 8/8, 7/8 HLA-matched unrelated, T-cell-replete haploidentical and umbilical cord blood transplantation; however, investigations of locus-specific effects are still scarce in the latter two settings. In unrelated transplantation, a specific role for mismatches at HLA-C and HLA-DPB1, and therein of permissive mismatches defined on the basis of T-cell alloreactivity and/or expression levels, in reducing relapse has been demonstrated in independent studies. This observation suggests new approaches to utilize HLA matching in unrelated donor searches, and the need for further research in the field. PMID:27000727

  6. A controlled comparative study of ibuprofen arginate versus conventional ibuprofen in the treatment of postoperative dental pain.

    PubMed

    Mehlisch, Donald R; Ardia, Alfredo; Pallotta, Teresa

    2002-08-01

    The analgesic efficacy of an arginine salt of ibuprofen was compared to one of the commercially available forms of conventional ibuprofen in a 500-patient clinical trial in postoperative dental pain. Patients were administered a single dose of ibuprofen arginate (200 mg or 400 mg), conventional ibuprofen (200 mg or 400 mg), orplacebo in this double-blind, randomized, parallel-group trial. Results demonstrated that ibuprofen arginate was a safe and effective analgesia that was superior to conventional ibuprofen in both the amount of pain relief achieved and the time to onset of pain relief. Onset of analgesia, assessed as the median amount of time to achieve meaningful pain relief, was reached after 32 and 31 minutes with ibuprofen arginate 200 and 400 mg, respectively, and 64 and 58 minutes with conventional ibuprofen 200 and 400 mg, respectively (p < 0.05). Patients treated with ibuprofen arginate rated its overall effectiveness higher than those patients treated with conventional ibuprofen. Adverse event profiles were similar across all treatment groups. PMID:12162473

  7. Improving the efficacy of conventional therapy by adding andrographolide sulfonate in the treatment of severe hand, foot, and mouth disease: a randomized controlled trial.

    PubMed

    Li, Xiuhui; Zhang, Chi; Shi, Qingsheng; Yang, Tong; Zhu, Qingxiong; Tian, Yimei; Lu, Cheng; Zhang, Zhiying; Jiang, Zhongsheng; Zhou, Hongying; Wen, Xiaofeng; Yang, Huasheng; Ding, Xiaorong; Liang, Lanchun; Liu, Yan; Wang, Yongyan; Lu, Aiping

    2013-01-01

    Background. Herb-derived compound andrographolide sulfonate (called Xiyanping injection) recommended control measure for severe hand, foot, and mouth disease (HFMD) by the Ministry of Health (China) during the 2010 epidemic. However, there is a lack of good quality evidence directly comparing the efficacy of Andrographolide Sulfonate combination therapy with conventional therapy. Methods. 230 patients were randomly assigned to 7-10 days of Andrographolide Sulfonate 5-10 mg/Kg/day and conventional therapy, or conventional therapy alone. Results. The major complications occurred less often after Andrographolide Sulfonate (2.6% versus 12.1%; risk difference [RD], 0.94; 95% CI, 0.28-1.61; P = 0.006). Median fever clearance times were 96 hours (CI, 80 to 126) for conventional therapy recipients and 48 hours (CI, 36 to 54) for Andrographolide Sulfonate combination-treated patients (χ(2) = 16.57, P < 0.001). The two groups did not differ in terms of HFMD-cause mortality (P = 1.00) and duration of hospitalization (P = 0.70). There was one death in conventional therapy group. No important adverse event was found in Andrographolide Sulfonate combination therapy group. Conclusions. The addition of Andrographolide Sulfonate to conventional therapy reduced the occurrence of major complications, fever clearance time, and the healing time of typical skin or oral mucosa lesions in children with severe HFMD. PMID:23401711

  8. Improving the Efficacy of Conventional Therapy by Adding Andrographolide Sulfonate in the Treatment of Severe Hand, Foot, and Mouth Disease: A Randomized Controlled Trial

    PubMed Central

    Li, Xiuhui; Zhang, Chi; Shi, Qingsheng; Yang, Tong; Zhu, Qingxiong; Tian, Yimei; Lu, Cheng; Zhang, Zhiying; Jiang, Zhongsheng; Zhou, Hongying; Wen, Xiaofeng; Yang, Huasheng; Ding, Xiaorong; Liang, Lanchun; Liu, Yan; Wang, Yongyan; Lu, Aiping

    2013-01-01

    Background. Herb-derived compound andrographolide sulfonate (called Xiyanping injection) recommended control measure for severe hand, foot, and mouth disease (HFMD) by the Ministry of Health (China) during the 2010 epidemic. However, there is a lack of good quality evidence directly comparing the efficacy of Andrographolide Sulfonate combination therapy with conventional therapy. Methods. 230 patients were randomly assigned to 7–10 days of Andrographolide Sulfonate 5–10 mg/Kg/day and conventional therapy, or conventional therapy alone. Results. The major complications occurred less often after Andrographolide Sulfonate (2.6% versus 12.1%; risk difference [RD], 0.94; 95% CI, 0.28–1.61; P = 0.006). Median fever clearance times were 96 hours (CI, 80 to 126) for conventional therapy recipients and 48 hours (CI, 36 to 54) for Andrographolide Sulfonate combination-treated patients (χ2 = 16.57, P < 0.001). The two groups did not differ in terms of HFMD-cause mortality (P = 1.00) and duration of hospitalization (P = 0.70). There was one death in conventional therapy group. No important adverse event was found in Andrographolide Sulfonate combination therapy group. Conclusions. The addition of Andrographolide Sulfonate to conventional therapy reduced the occurrence of major complications, fever clearance time, and the healing time of typical skin or oral mucosa lesions in children with severe HFMD. PMID:23401711

  9. A Method for Integrating Thrust-Vectoring and Actuated Forebody Strakes with Conventional Aerodynamic Controls on a High-Performance Fighter Airplane

    NASA Technical Reports Server (NTRS)

    Lallman, Frederick J.; Davidson, John B.; Murphy, Patrick C.

    1998-01-01

    A method, called pseudo controls, of integrating several airplane controls to achieve cooperative operation is presented. The method eliminates conflicting control motions, minimizes the number of feedback control gains, and reduces the complication of feedback gain schedules. The method is applied to the lateral/directional controls of a modified high-performance airplane. The airplane has a conventional set of aerodynamic controls, an experimental set of thrust-vectoring controls, and an experimental set of actuated forebody strakes. The experimental controls give the airplane additional control power for enhanced stability and maneuvering capabilities while flying over an expanded envelope, especially at high angles of attack. The flight controls are scheduled to generate independent body-axis control moments. These control moments are coordinated to produce stability-axis angular accelerations. Inertial coupling moments are compensated. Thrust-vectoring controls are engaged according to their effectiveness relative to that of the aerodynamic controls. Vane-relief logic removes steady and slowly varying commands from the thrust-vectoring controls to alleviate heating of the thrust turning devices. The actuated forebody strakes are engaged at high angles of attack. This report presents the forward-loop elements of a flight control system that positions the flight controls according to the desired stability-axis accelerations. This report does not include the generation of the required angular acceleration commands by means of pilot controls or the feedback of sensed airplane motions.

  10. Beam halo in mismatched proton beams.

    SciTech Connect

    Wangler, Thomas P.,; Allen, C. K.; Chan, D.; Colestock, P. L. ,; Crandall, K. R.; Qiang, J.; Garnett, R. W.; Lysenko, W. P.; Gilpatrick, J. D.; Schneider, J. D.; Schulze, M. E.; Sheffield, R. L.; Smith, H. V.

    2002-01-01

    Progress was made during the past decade towards a better understanding of halo formation caused by beam mismatch in high-intensity beams. To test these ideas an experiment was carried out at Los Alamos with proton beams in a 52-quadrupole focusing channel. Rms emittances and beam widths were obtained from measured beam profiles for comparison with the maximum emittance growth predictions of a free-energy model and the maximum haloamplitude predictions of a particle-core model. The experimental results are also compared with multiparticle simulations. In this paper we will present the experimental results and discuss the implications with respect to the validity of both the models and the simulations. Keywords: beam halo, emittance growth, beam profiles, simulations, space charge, mismatch

  11. Grammar, Punctuation, and Spelling: Controlling the Conventions of Written English at Ages 9, 13, and 17. The Nation's Report Card.

    ERIC Educational Resources Information Center

    Applebee, Arthur N.; And Others

    The National Assessment of Educational Progress (NAEP) administered a writing task to nationally representative samples of 9-, 13-, and 17-year-old students in order to assess their abilities to use the conventions of written English. Approximately 2,000 students at each age level completed this task as part of NAEP's 1984 writing assessment.…

  12. Laparoendoscopic single-site surgery (LESS) versus conventional laparoscopic surgery for adnexal preservation: a randomized controlled study

    PubMed Central

    Cho, Yeon Jean; Kim, Mi-La; Lee, Soo Yoon; Lee, Hee Suk; Kim, Joo Myoung; Joo, Kwan Young

    2012-01-01

    Objective To compare the operative outcomes, postoperative pain, and subsequent convalescence after laparoendoscopic single-site surgery (LESS) or conventional laparoscopic surgery for adnexal preservation. Study design From December 2009 to September 2010, 63 patients underwent LESS (n = 33) or a conventional laparoscopic surgery (n = 30) for cyst enucleation. The overall operative outcomes including postoperative pain measurement using the visual analog scale (VAS) were evaluated (time points 6, 24, and 24 hours). The convalescence data included data obtained from questionnaires on the need for analgesics and on patient-reported time to recovery end points. Results The preoperative characteristics did not significantly differ between the two groups. The postoperative hemoglobin drop was higher in the LESS group than in the conventional laparoscopic surgery group (P = 0.048). Postoperative pain at each VAS time point, oral analgesic requirement, intramuscular analgesic requirement, and the number of days until return to work were similar in both groups. Conclusion In adnexa-preserving surgery performed in reproductive-age women, the operative outcomes, including satisfaction of the patients and convalescence after surgery, are comparable for LESS and conventional laparoscopy. LESS may be a feasible and a promising alternative method for scarless abdominal surgery in the treatment of young women with adnexal cysts PMID:22448110

  13. Comparison of autologous serum eye drops with conventional therapy in a randomised controlled crossover trial for ocular surface disease

    PubMed Central

    Noble, B A; Loh, R S K; MacLennan, S; Pesudovs, K; Reynolds, A; Bridges, L R; Burr, J; Stewart, O; Quereshi, S

    2004-01-01

    Aims: To evaluate the efficacy of 50% autologous serum drops against conventional treatment in ocular surface disorders refractory to normal treatments in a prospective randomised crossover trial. Method: Patients fulfilling ophthalmological and haematological entry criteria were randomised to either 3 months of autologous serum 50% followed by 3 months of their conventional treatment, or 3 months of conventional treatment, followed by 3 months of autologous serum. Clinical assessments, including Schirmer’s test, rose Bengal, and fluorescein staining, were carried out on entry and at monthly intervals. Impression cytology was performed at entry, 3 and 6 months. Grading was carried out on degrees of squamous metaplasia and goblet cell density. Subjective comfort was recorded daily using the “faces” scale. These categorical scores were converted to linear measurement using Rasch analysis. Statistical analysis was carried out using Wilcoxon’s signed rank test and ANOVA. Results: 16 patients were recruited with 31 eyes studied. The ocular surface diseases chiefly included Sjögren’s syndrome (n = 6) and keratoconjunctivitis sicca (n = 5). Impression cytology available in 25 of 31 eyes showed significant improvement on serum treatment, p<0.02. Rasch weighted faces scores were statistically significantly better with serum, p<0.01. Conclusion: The results of this randomised study provide further evidence of the beneficial effects of autologous serum in severe ocular surface disorders. For most of these patients, autologous serum was superior to conventional treatment for improving ocular surface health and subjective comfort. PMID:15090417

  14. Footprint mismatch in lumbar total disc arthroplasty.

    PubMed

    Gstoettner, Michaela; Michaela, Gstoettner; Heider, Denise; Denise, Heider; Liebensteiner, Michael; Bach, Christian Michael; Michael, Bach Christian

    2008-11-01

    Lumbar disc arthroplasty has become a popular modality for the treatment of degenerative disc disease. The dimensions of the implants are based on early published geometrical measurements of vertebrae; the majority of these were cadaver studies. The fit of the prosthesis in the intervertebral space is of utmost importance. An undersized implant may lead to subsidence, loosening and biomechanical failure due to an incorrect center of rotation. The aim of the present study was to measure the dimensions of lumbar vertebrae based on CT scans and assess the accuracy of match in currently available lumbar disc prostheses. A total of 240 endplates of 120 vertebrae were included in the study. The sagittal and mediolateral diameter of the upper and lower endplates were measured using a digital measuring system. For the levels L4/L5 and L5/S1, an inappropriate size match was noted in 98.8% (Prodisc L) and 97.6% (Charite) with regard to the anteroposterior diameter. Mismatch in the anterior mediolateral diameter was noted in 79.3% (Prodisc L) and 51.2% (Charite) while mismatch in the posterior mediolateral diameter was observed in 91.5% (Prodisc L) and 78% (Charite) of the endplates. Surgeons and manufacturers should be aware of the size mismatch of currently available lumbar disc prostheses, which may endanger the safety and efficacy of the procedure. Larger footprints of currently available total disc arthroplasties are required. PMID:18791748

  15. Footprint mismatch in lumbar total disc arthroplasty

    PubMed Central

    Michaela, Gstoettner; Denise, Heider; Liebensteiner, Michael

    2008-01-01

    Lumbar disc arthroplasty has become a popular modality for the treatment of degenerative disc disease. The dimensions of the implants are based on early published geometrical measurements of vertebrae; the majority of these were cadaver studies. The fit of the prosthesis in the intervertebral space is of utmost importance. An undersized implant may lead to subsidence, loosening and biomechanical failure due to an incorrect center of rotation. The aim of the present study was to measure the dimensions of lumbar vertebrae based on CT scans and assess the accuracy of match in currently available lumbar disc prostheses. A total of 240 endplates of 120 vertebrae were included in the study. The sagittal and mediolateral diameter of the upper and lower endplates were measured using a digital measuring system. For the levels L4/L5 and L5/S1, an inappropriate size match was noted in 98.8% (Prodisc L) and 97.6% (Charite) with regard to the anteroposterior diameter. Mismatch in the anterior mediolateral diameter was noted in 79.3% (Prodisc L) and 51.2% (Charite) while mismatch in the posterior mediolateral diameter was observed in 91.5% (Prodisc L) and 78% (Charite) of the endplates. Surgeons and manufacturers should be aware of the size mismatch of currently available lumbar disc prostheses, which may endanger the safety and efficacy of the procedure. Larger footprints of currently available total disc arthroplasties are required. PMID:18791748

  16. Proteasome inhibition rescues clinically significant unstable variants of the mismatch repair protein Msh2

    PubMed Central

    Arlow, Tim; Scott, Kristan; Wagenseller, Aubrey; Gammie, Alison

    2013-01-01

    MSH2 is required for DNA mismatch repair recognition in eukaryotes. Deleterious mutations in human MSH2 account for approximately half of the alleles associated with a common hereditary cancer syndrome. Previously, we characterized clinically identified MSH2 missense mutations, using yeast as a model system, and found that the most common cause of defective DNA mismatch repair was low levels of the variant Msh2 proteins. Here, we show that increased protein turnover is responsible for the reduced cellular levels. Increasing gene dosage of more than half of the missense alleles fully restored function. A titration experiment revealed that raising the expression level of one variant to less than wild-type levels restored mismatch repair, suggesting that overexpression is not always required to regain function. We found that the ubiquitin-mediated proteasome degradation pathway is the major mechanism for increased turnover of the Msh2 variants and identified the primary ubiquitin ligase as San1. Deletion of San1 restored protein levels for all but one variant, but did not elevate wild-type Msh2 levels. The unstable variants interacted with San1, whereas wild-type Msh2 did not. Additionally, san1Δ suppressed the mismatch repair defect of unstable variants. Of medical significance, the clinically approved drug Bortezomib partially restored protein levels and mismatch repair function for low-level variants and reversed the resistance to cisplatin, a common chemotherapeutic. Our results provide the foundation for an innovative therapeutic regime for certain mismatch-repair-defective cancers that are refractory to conventional chemotherapies. PMID:23248292

  17. A meta-analysis of randomised controlled trials comparing conventional to minimally invasive approaches for repair of an Achilles tendon rupture.

    PubMed

    McMahon, Samuel E; Smith, Toby O; Hing, Caroline B

    2011-12-01

    Achilles tendon ruptures are a common injury afflicting predominantly the young male occasional sportsman. Previous studies have shown that outcome is better with surgical repair for the young active patient. There is no consensus as to whether there is a difference in outcome between open and percutaneous minimally invasive surgery (MIS). A meta-analysis was undertaken to compare the clinical outcomes of MIS with conventional open surgical repair. Six randomised controlled trials of 277 Achilles tendon repairs were eligible for review. This included 136 minimally invasive repairs and 141 conventional open repairs. On analysis, there was no significant difference between the two surgical approaches in respect to the incidence of re-rupture, tissue adhesion, sural nerve injury, deep infection and deep vein thrombosis (p>0.05). However, MIS had a significantly reduced risk of superficial wound infection, with three times greater patient satisfaction for good to excellent results compared with conventional open surgical approaches. PMID:22017889

  18. A randomised controlled trial of mini incision or conventional incision for saphenous vein harvesting in patients undergoing myocardial revascularization

    PubMed Central

    Gontijo de Deus, Kleber; Diogo Filho, Augusto; Cesar Santos, Paulo

    2016-01-01

    Objective Compare the evolution regarding the complications concerning two types of incision (conventional × mini-incision), for saphenectomy in patients that go under myocardial revascularization or otherwise known as coronary artery bypass surgery. Methods In January 2012 to August 2013, 66 patients were prospectively selected for coronary artery bypass with cardiopulmonary bypass surgery. These were divided into two groups: Conventional and Mini-Incision, with 33 patients in each group chosen in a random fashion and with knowledge of which technique to be used being presented only at the start of the surgery. In the conventional group, the patients received an incision to the lower member of 7–10 cm. The patients in the Mini-Incision group received an incision to the lower member of 3–4 cm, both performed without the use of any special material. Results The groups were similar in terms of clinical data and in the preoperative period. Males made up a greater part of the group with 63.7% and 81.9% in groups C and M, respectively. Among the complications analysed, edema (p = 0.011), hematoma (p = 0.020), dehiscence (p = 0.012) and infection (p = 0.012), were significantly greater in group C when compared to group M. When the matter comes to the variable in relation to the risk of Surgical Site Infections (SSI), no difference was found between the groups. Conclusion Coronary artery bypass surgery with mini-incision for saphenectomy, demonstrated a lower rate for preoperative complications when compared to saphenectomy under conventional incision procedures. PMID:27006766

  19. Controlled trial of a continuous irrigation suction catheter vs conventional intermittent suction catheter in clearing bronchial secretions from ventilated patients.

    PubMed

    Isea, J O; Poyant, D; O'Donnell, C; Faling, L J; Karlinsky, J; Celli, B R

    1993-04-01

    Continuous irrigation-suction catheter (Irri-cath) is a double-lumen device that allows for simultaneous saline solution infusion and aspiration. This system may theoretically be more effective than conventional dry intermittent suction due to its vortex principle. To test this hypothesis, we performed 200 suction maneuvers in 20 ventilated patients. Identically shaped catheters were used in a randomized sequence. For the same individual, we used equal instilled saline solution volume (40 ml), vacuum pressure (-180 cm H2O), and ventilatory parameters. Effectiveness of suction was determined by measuring the total aspirated volume, the dry lyophilized weight of secretion, the corrected dry weight (dry weight-weight of instilled salt), and protein concentration. No difference in heart rate, respiratory frequency, O2 saturation, systemic blood pressure, peak inspiratory pressure, or patient discomfort was found when the two modalities were compared; however, the total volume of secretions collected, the dry weight, the corrected dry weight, and the protein concentration were significantly higher with continuous irrigation suction catheter when compared with the conventional method (p < 0.05). The suction time was shorter with the Irri-cath (p < 0.05). We conclude that the Irri-Cath is more effective than conventional intermittent suction catheter in clearing bronchial secretions in patients on mechanical ventilation. PMID:8131470

  20. Study of charge control and gate tunneling in a ferroelectric-oxide-silicon field effect transistor: Comparison with a conventional metal-oxide-silicon structure

    NASA Astrophysics Data System (ADS)

    Lin, Yih-Yin; Zhang, Yifei; Singh, Jasprit; York, Robert; Mishra, Umesh

    2001-02-01

    It is known that conventional metal-oxide-silicon (MOS) devices will have gate tunneling related problems at very thin oxide thicknesses. Various high-dielectric-constant materials are being examined to suppress the gate currents. In this article we present theoretical results of a charge control and gate tunneling model for a ferroelectric-oxide-silicon field effect transistor and compare them to results for a conventional MOS device. The potential of high polarization charge to induce inversion without doping and high dielectric constant to suppress tunneling current is explored. The model is based on a self-consistent solution of the quantum problem and includes the ferroelectric hysteresis response self-consistently. We show that the polarization charge associated with ferroelectrics can allow greater controllability of the inversion layer charge density. Also the high dielectric constant of ferroelectrics results in greatly suppressed gate current.

  1. Mismatched DNTP Incorporation By DNA Polymerase Beta Does Not Proceed Via Globally Different Conformational Pathways

    SciTech Connect

    Tang, K.-H.; Niebuhr, M.; Tung, C.-S.; Chan, H.-c.; Chou, C.-C.; Tsai, M.-D.

    2009-05-26

    Understanding how DNA polymerases control fidelity requires elucidation of the mechanisms of matched and mismatched dNTP incorporations. Little is known about the latter because mismatched complexes do not crystallize readily. In this report, we employed small-angle X-ray scattering (SAXS) and structural modeling to probe the conformations of different intermediate states of mammalian DNA polymerase {beta} (Pol {beta}) in its wild-type and an error-prone variant, I260Q. Our structural results indicate that the mismatched ternary complex lies in-between the open and the closed forms, but more closely resembles the open form for WT and the closed form for I260Q. On the basis of molecular modeling, this over-stabilization of mismatched ternary complex of I260Q is likely caused by formation of a hydrogen bonding network between the side chains of Gln{sup 260}, Tyr{sup 296}, Glu{sup 295} and Arg{sup 258}, freeing up Asp{sup 192} to coordinate MgdNTP. These results argue against recent reports suggesting that mismatched dNTP incorporations follow a conformational path distinctly different from that of matched dNTP incorporation, or that its conformational closing is a major contributor to fidelity.

  2. Tretinoin Nanogel 0.025% Versus Conventional Gel 0.025% in Patients with Acne Vulgaris: A Randomized, Active Controlled, Multicentre, Parallel Group, Phase IV Clinical Trial

    PubMed Central

    Chandrashekhar, B S; Anitha, M.; Ruparelia, Mukesh; Vaidya, Pradyumna; Aamir, Riyaz; Shah, Sunil; Thilak, S; Aurangabadkar, Sanjeev; Pal, Sandeep; Saraswat, Abir

    2015-01-01

    Background: Conventional topical tretinoin formulation is often associated with local adverse events. Nanogel formulation of tretinoin has good physical stability and enables good penetration of tretinoin into the pilo-sebaceous glands. Aim: The present study was conducted to assess the efficacy and safety of a nanogel formulation of tretinoin as compared to its conventional gel formulation in the treatment of acne vulgaris of the face. Materials and Methods: This randomized, active controlled, multicentric, phase IV clinical trial evaluated the treatment of patients with acne vulgaris of the face by the two gel formulations locally applied once daily at night for 12 wk. Acne lesion counts (inflammatory, non-inflammatory & total) and severity grading were carried out on the monthly scheduled visits along with the tolerability assessments. Results: A total of 207 patients were randomized in the study. Reductions in the total (72.9% vs. 65.0%; p = 0.03) and inflammatory (78.1% vs. 66.9%; p = 0.02) acne lesions were reported to be significantly greater with the nanogel formulation as compared to the conventional gel formulation. Local adverse events were significantly less (p = 0.04) in the nanogel group (13.3%) as compared to the conventional gel group (24.7%). Dryness was the most common adverse event reported in both the treatment groups while peeling of skin, burning sensation and photosensitivity were reported in patients using the conventional gel only. Conclusion: In the treatment of acne vulgaris of the face, tretinoin nanogel formulation appears to be more effective and better tolerated than the conventional gel formulation. PMID:25738069

  3. A low-fat vegan diet and a conventional diabetes diet in the treatment of type 2 diabetes: a randomized, controlled, 74-wk clinical trial1234

    PubMed Central

    Barnard, Neal D; Cohen, Joshua; Jenkins, David JA; Turner-McGrievy, Gabrielle; Gloede, Lise; Green, Amber; Ferdowsian, Hope

    2009-01-01

    Background: Low-fat vegetarian and vegan diets are associated with weight loss, increased insulin sensitivity, and improved cardiovascular health. Objective: We compared the effects of a low-fat vegan diet and conventional diabetes diet recommendations on glycemia, weight, and plasma lipids. Design: Free-living individuals with type 2 diabetes were randomly assigned to a low-fat vegan diet (n = 49) or a diet following 2003 American Diabetes Association guidelines (conventional, n = 50) for 74 wk. Glycated hemoglobin (Hb A1c) and plasma lipids were assessed at weeks 0, 11, 22, 35, 48, 61, and 74. Weight was measured at weeks 0, 22, and 74. Results: Weight loss was significant within each diet group but not significantly different between groups (−4.4 kg in the vegan group and −3.0 kg in the conventional diet group, P = 0.25) and related significantly to Hb A1c changes (r = 0.50, P = 0.001). Hb A1c changes from baseline to 74 wk or last available values were −0.34 and −0.14 for vegan and conventional diets, respectively (P = 0.43). Hb A1c changes from baseline to last available value or last value before any medication adjustment were −0.40 and 0.01 for vegan and conventional diets, respectively (P = 0.03). In analyses before alterations in lipid-lowering medications, total cholesterol decreased by 20.4 and 6.8 mg/dL in the vegan and conventional diet groups, respectively (P = 0.01); LDL cholesterol decreased by 13.5 and 3.4 mg/dL in the vegan and conventional groups, respectively (P = 0.03). Conclusions: Both diets were associated with sustained reductions in weight and plasma lipid concentrations. In an analysis controlling for medication changes, a low-fat vegan diet appeared to improve glycemia and plasma lipids more than did conventional diabetes diet recommendations. Whether the observed differences provide clinical benefit for the macro- or microvascular complications of diabetes remains to be established. This trial was registered at clinicaltrials

  4. Recovery act. Characterizing structural controls of EGS-candidate and conventional geothermal reservoirs in the Great Basin. Developing successful exploration strategies in extended terranes

    SciTech Connect

    Faulds, James

    2015-06-25

    We conducted a comprehensive analysis of the structural controls of geothermal systems within the Great Basin and adjacent regions. Our main objectives were to: 1) Produce a catalogue of favorable structural environments and models for geothermal systems. 2) Improve site-specific targeting of geothermal resources through detailed studies of representative sites, which included innovative techniques of slip tendency analysis of faults and 3D modeling. 3) Compare and contrast the structural controls and models in different tectonic settings. 4) Synthesize data and develop methodologies for enhancement of exploration strategies for conventional and EGS systems, reduction in the risk of drilling non-productive wells, and selecting the best EGS sites.

  5. The Impact of Educational Mismatch on Firm Productivity: Evidence from Linked Panel Data

    ERIC Educational Resources Information Center

    Kampelmann, Stephan; Rycx, Francois

    2012-01-01

    We provide first evidence regarding the direct impact of educational mismatch on firm productivity. To do so, we rely on representative linked employer-employee panel data for Belgium covering the period 1999-2006. Controlling for simultaneity issues, time-invariant unobserved workplace characteristics, cohort effects and dynamics in the…

  6. Use of Green Infrastructure Integrated with Conventional Gray Infrastructure for Combined Sewer Overflow Control: Kansas City, MO

    EPA Science Inventory

    Advanced design concepts such as Low Impact Development (LID) and Green Solutions (or upland runoff control techniques) are currently being encouraged by the United States Environmental Protection Agency (EPA) as a management practice to contain and control stormwater at the lot ...

  7. Mismatch removal via coherent spatial relations

    NASA Astrophysics Data System (ADS)

    Chen, Jun; Ma, Jiayi; Yang, Changcai; Tian, Jinwen

    2014-07-01

    We propose a method for removing mismatches from the given putative point correspondences in image pairs based on "coherent spatial relations." Under the Bayesian framework, we formulate our approach as a maximum likelihood problem and solve a coherent spatial relation between the putative point correspondences using an expectation-maximization (EM) algorithm. Our approach associates each point correspondence with a latent variable indicating it as being either an inlier or an outlier, and alternatively estimates the inlier set and recovers the coherent spatial relation. It can handle not only the case of image pairs with rigid motions but also the case of image pairs with nonrigid motions. To parameterize the coherent spatial relation, we choose two-view geometry and thin-plate spline as models for rigid and nonrigid cases, respectively. The mismatches could be successfully removed via the coherent spatial relations after the EM algorithm converges. The quantitative results on various experimental data demonstrate that our method outperforms many state-of-the-art methods, it is not affected by low initial correct match percentages, and is robust to most geometric transformations including a large viewing angle, image rotation, and affine transformation.

  8. Repair of mismatched basepairs in mammalian DNA

    SciTech Connect

    Taylor, J.H.; Hare, J.T.

    1991-08-01

    We have concentrated on three specific areas of our research plan. Our greatest emphasis is on the role of single strand nicks in influencing template strand selection in mismatch repair. We have found, that the ability of a nick in one strand to influence which strand is repaired is not a simple function of distance from the mismatched site but rather that an hot spot where a nick is more likely to have an influence can exist. The second line was production of single-genotype heteroduplexes in order to examine independently the repair of T/G and A/C mispairs within the same sequence context as in our mixed mispair preparations. We have shown preparations of supercoiled heteroduplex can be prepared that were exclusively T/G or exclusively A/C at the mispair site. The third effort has been to understand the difference in repair bias of different cell lines or different transfection conditions as it may relate to different repair systems in the cell. We have identified some of the sources of variation, including cell cycle position. We hope to continue this work to more precisely identify the phase of the cell cycle.

  9. Reducing measurement scale mismatch to improve surface energy flux estimation

    NASA Astrophysics Data System (ADS)

    Iwema, Joost; Rosolem, Rafael; Rahman, Mostaquimur; Blyth, Eleanor; Wagener, Thorsten

    2016-04-01

    Soil moisture importantly controls land surface processes such as energy and water partitioning. A good understanding of these controls is needed especially when recognizing the challenges in providing accurate hyper-resolution hydrometeorological simulations at sub-kilometre scales. Soil moisture controlling factors can, however, differ at distinct scales. In addition, some parameters in land surface models are still often prescribed based on observations obtained at another scale not necessarily employed by such models (e.g., soil properties obtained from lab samples used in regional simulations). To minimize such effects, parameters can be constrained with local data from Eddy-Covariance (EC) towers (i.e., latent and sensible heat fluxes) and Point Scale (PS) soil moisture observations (e.g., TDR). However, measurement scales represented by EC and PS still differ substantially. Here we use the fact that Cosmic-Ray Neutron Sensors (CRNS) estimate soil moisture at horizontal footprint similar to that of EC fluxes to help answer the following question: Does reduced observation scale mismatch yield better soil moisture - surface fluxes representation in land surface models? To answer this question we analysed soil moisture and surface fluxes measurements from twelve COSMOS-Ameriflux sites in the USA characterized by distinct climate, soils and vegetation types. We calibrated model parameters of the Joint UK Land Environment Simulator (JULES) against PS and CRNS soil moisture data, respectively. We analysed the improvement in soil moisture estimation compared to uncalibrated model simulations and then evaluated the degree of improvement in surface fluxes before and after calibration experiments. Preliminary results suggest that a more accurate representation of soil moisture dynamics is achieved when calibrating against observed soil moisture and further improvement obtained with CRNS relative to PS. However, our results also suggest that a more accurate

  10. Model Mismatch Paradigm for Probe based Nanoscale Imaging

    NASA Astrophysics Data System (ADS)

    Agarwal, Pranav

    deals with real time detection and reduction of spurious areas in the image which are also known as probe-loss areas. These areas become severely detrimental during high speed operations. The detection strategy is based on thresholding of a distance measure, which captures the difference between sensor models in absence and presence of probe-loss. A switching gain control strategy based on the output of a Kalman Filter is used to reduce probe-loss areas in real time. The efficacy of this technique is demonstrated through experimental results showing increased image fidelity at scan rates that are 10 times faster than conventional scan rates. The second contribution of this thesis deals with developing multi-frequency input excitation strategy and deriving a bias compensated adaptive parameter estimation strategy to determine the instantaneous equivalent cantilever model. This is used to address the challenge of quantitative imaging at high bandwidth operation by relating the estimated plant coefficients to conservative and dissipative components of tip-sample interaction. The efficacy of the technique is demonstrated for quantitative material characterization of a polymer sample, resulting in material information not previously obtainable during dynamic mode operation. This information is obtained at speeds which are two orders faster than existing techniques. Quantitative verification strategies for the accuracy of estimated parameters are presented. The third contribution of this thesis deals with developing real time tractable models and characterization methodology for an electrostatically actuated MEMS cantilever with an integrated solid state thermal sensor. Appropriate modeling assumptions are made to delineate various nonlinear forces on the cantilever viz. electrostatic force, tip-sample interaction force and capacitive coupling. Experimental strategy is presented to measure the thermal sensing transfer function from DC-100kHz. A quantitative match between

  11. Quantum noise in parametric amplification under phase-mismatched conditions

    NASA Astrophysics Data System (ADS)

    Inoue, K.

    2016-05-01

    This paper studies quantum noise in parametric amplification under phase-mismatched conditions. The equations of motion of the quantum-mechanical field operators, which include phase mismatch under unsaturated conditions are first derived from the Heisenberg equation. Next, the noise figure is evaluated using the solutions of the derived equations. The results indicate that phase mismatch scarcely affects noise property in phase-insensitive amplification while it has a notable effect in case of phase-sensitive amplification.

  12. Imaging of biomaterials in liquids: a comparison between conventional and Q-controlled amplitude modulation ('tapping mode') atomic force microscopy

    NASA Astrophysics Data System (ADS)

    Ebeling, D.; Hölscher, H.; Fuchs, H.; Anczykowski, B.; Schwarz, U. D.

    2006-04-01

    Lambda phage DNA and DPPC thin films are imaged in liquids by atomic force microscopy applying the amplitude modulation mode ('tapping mode') with active enhancement of the Q-factor by a 'Q-control' electronics. The topography of the resulting images is compared with images obtained without active Q-control. To enable a meaningful comparison, individual scan lines are alternately recorded with and without Q-factor enhancement using scan parameters optimized for each mode separately. As the major finding, significant height differences of topographical features are observed between the two modes. The heights measured with active Q-control are reproducibly higher compared to the ones observed without Q enhancement. This effect is attributed to the reduction of tip-sample forces by Q-control.

  13. Effect of a Brown Rice Based Vegan Diet and Conventional Diabetic Diet on Glycemic Control of Patients with Type 2 Diabetes: A 12-Week Randomized Clinical Trial

    PubMed Central

    Lee, Yu-Mi; Kim, Se-A; Lee, In-Kyu; Kim, Jung-Guk; Park, Keun-Gyu; Jeong, Ji-Yun; Jeon, Jae-Han; Shin, Ji-Yeon; Lee, Duk-Hee

    2016-01-01

    Objective Several intervention studies have suggested that vegetarian or vegan diets have clinical benefits, particularly in terms of glycemic control, in patients with type 2 diabetes (T2D); however, no randomized controlled trial has been conducted in Asians who more commonly depend on plant-based foods, as compared to Western populations. Here, we aimed to compare the effect of a vegan diet and conventional diabetic diet on glycemic control among Korean individuals. Materials and Methods Participants diagnosed with T2D were randomly assigned to follow either a vegan diet (excluding animal-based food including fish; n = 46) or a conventional diet recommended by the Korean Diabetes Association 2011 (n = 47) for 12 weeks. HbA1c levels were measured at weeks 0, 4, and 12, and the primary study endpoint was the change in HbA1c levels over 12 weeks. Results The mean HbA1c levels at weeks 0, 4, and 12 were 7.7%, 7.2%, and 7.1% in the vegan group, and 7.4%, 7.2%, and 7.2% in the conventional group, respectively. Although both groups showed significant reductions in HbA1C levels, the reductions were larger in the vegan group than in the conventional group (-0.5% vs. -0.2%; p-for-interaction = 0.017). When only considering participants with high compliance, the difference in HbA1c level reduction between the groups was found to be larger (-0.9% vs. -0.3%). The beneficial effect of vegan diets was noted even after adjusting for changes in total energy intake or waist circumference over the 12 weeks. Conclusion Both diets led to reductions in HbA1c levels; however, glycemic control was better with the vegan diet than with the conventional diet. Thus, the dietary guidelines for patients with T2D should include a vegan diet for the better management and treatment. However, further studies are needed to evaluate the long-term effects of a vegan diet, and to identify potential explanations of the underlying mechanisms. Trial Registration CRiS KCT0001771 PMID:27253526

  14. Associations of Cognitive Function Scores with Carbon Dioxide, Ventilation, and Volatile Organic Compound Exposures in Office Workers: A Controlled Exposure Study of Green and Conventional Office Environments

    PubMed Central

    Allen, Joseph G.; MacNaughton, Piers; Satish, Usha; Santanam, Suresh; Vallarino, Jose; Spengler, John D.

    2015-01-01

    Background: The indoor built environment plays a critical role in our overall well-being because of both the amount of time we spend indoors (~90%) and the ability of buildings to positively or negatively influence our health. The advent of sustainable design or green building strategies reinvigorated questions regarding the specific factors in buildings that lead to optimized conditions for health and productivity. Objective: We simulated indoor environmental quality (IEQ) conditions in “Green” and “Conventional” buildings and evaluated the impacts on an objective measure of human performance: higher-order cognitive function. Methods: Twenty-four participants spent 6 full work days (0900–1700 hours) in an environmentally controlled office space, blinded to test conditions. On different days, they were exposed to IEQ conditions representative of Conventional [high concentrations of volatile organic compounds (VOCs)] and Green (low concentrations of VOCs) office buildings in the United States. Additional conditions simulated a Green building with a high outdoor air ventilation rate (labeled Green+) and artificially elevated carbon dioxide (CO2) levels independent of ventilation. Results: On average, cognitive scores were 61% higher on the Green building day and 101% higher on the two Green+ building days than on the Conventional building day (p < 0.0001). VOCs and CO2 were independently associated with cognitive scores. Conclusions: Cognitive function scores were significantly better under Green+ building conditions than in the Conventional building conditions for all nine functional domains. These findings have wide-ranging implications because this study was designed to reflect conditions that are commonly encountered every day in many indoor environments. Citation: Allen JG, MacNaughton P, Satish U, Santanam S, Vallarino J, Spengler JD. 2016. Associations of cognitive function scores with carbon dioxide, ventilation, and volatile organic compound

  15. Educational mismatch and health status among foreign-born workers in Sweden.

    PubMed

    Dunlavy, A C; Garcy, A M; Rostila, M

    2016-04-01

    Foreign-born workers have been shown to experience poorer working conditions than native-born workers. Yet relationships between health and educational mismatch have been largely overlooked among foreign-born workers. This study uses objective and self-reported measures of educational mismatch to compare the prevalence of educational mismatch among native (n = 2359) and foreign-born (n = 1789) workers in Sweden and to examine associations between educational mismatch and poor self-rated health. Findings from weighted multivariate logistic regression which controlled for social position and individual-level demographic characteristics suggested that over-educated foreign-born workers had greater odds ratios for poor-self rated health compared to native-born matched workers. This association was particularly evident among men (OR = 2.14, 95% CI: 1.04-4.39) and women (OR = 2.13, 95% CI: 1.12-4.03) from countries outside of Western Europe, North America, and Australia/New Zealand. Associations between under-education and poor-self rated health were also found among women from countries outside of Western Europe, North America, and Australia/New Zealand (OR = 2.02, 95% CI: 1.27-3.18). These findings suggest that educational mismatch may be an important work-related social determinant of health among foreign-born workers. Future studies are needed to examine the effects of long-term versus short-term states of educational mismatch on health and to study relationships over time. PMID:26943012

  16. Probing DNA hybridization efficiency and single base mismatch by X-ray photoelectron spectroscopy.

    PubMed

    Liu, Zheng-Chun; Zhang, Xin; He, Nong-Yue; Lu, Zu-Hong; Chen, Zhen-Cheng

    2009-07-01

    We demonstrated the use of X-ray photoelectron spectroscopy (XPS) to study DNA hybridization. Target DNA labeled with hexachloro-fluorescein (HEX) was hybridized to DNA arrays with four different probes. Each probe dot of the hybridized arrays was detected with XPS. The XPS Cl2p peak areas were found to decrease with an increase in mismatched bases in DNA probes. The Cl2p core-level peak area ratio of a probe perfectly matched to one, two and three base-mismatched probes accorded well with the results of conventional fluorescent imaging, which shows that XPS is a potential tool for analyzing DNA arrays. The DNA arrays' hybridization efficiency was assessed by the molar ratio of chlorine to phosphorus in a DNA strand, which was determined from the relevant XPS Cl2p and P2p core-level peak areas after hybridization. This could provide a new method to detect DNA hybridization efficiency. PMID:19282155

  17. Iterative Robust Capon Beamforming with Adaptively Updated Array Steering Vector Mismatch Levels

    PubMed Central

    Sun, Liguo

    2014-01-01

    The performance of the conventional adaptive beamformer is sensitive to the array steering vector (ASV) mismatch. And the output signal-to interference and noise ratio (SINR) suffers deterioration, especially in the presence of large direction of arrival (DOA) error. To improve the robustness of traditional approach, we propose a new approach to iteratively search the ASV of the desired signal based on the robust capon beamformer (RCB) with adaptively updated uncertainty levels, which are derived in the form of quadratically constrained quadratic programming (QCQP) problem based on the subspace projection theory. The estimated levels in this iterative beamformer present the trend of decreasing. Additionally, other array imperfections also degrade the performance of beamformer in practice. To cover several kinds of mismatches together, the adaptive flat ellipsoid models are introduced in our method as tight as possible. In the simulations, our beamformer is compared with other methods and its excellent performance is demonstrated via the numerical examples. PMID:27355008

  18. Tobacco Industry attempts to counter the World Bank Report Curbing the Epidemic and obstruct the WHO Framework Convention on Tobacco Control

    PubMed Central

    Mamudu, Hadii M; Hammond, Ross

    2008-01-01

    In 1999 the World Bank published a landmark study on the economics of tobacco control, Curbing the Epidemic: Governments and the Economics of Tobacco Control (CTE), which concluded that tobacco control brings unprecedented health benefits without harming economies, threatening the transnational tobacco companies’ ability to use economic arguments to dissuade governments from enacting tobacco control policies and supporting the WHO Framework Convention on Tobacco Control (FCTC). We used tobacco industry documents to analyze how tobacco companies worked to discredit CTE. They hired public relations firms, had academics critique CTE, hired consultants to produce “independent” estimates of the importance of tobacco to national economies, and worked through front groups, particularly the International Tobacco Growers’ Association, to question CTE’s findings. These efforts failed, and the report remains an authoritative economic analysis of global tobacco control during the ongoing FCTC negotiations. The industry’s failure suggests that the World Bank should continue their analytic work on the economics of tobacco control and make tobacco control part of its development agenda. PMID:18950924

  19. Mismatch repair deficiency testing in clinical practice.

    PubMed

    Buza, Natalia; Ziai, James; Hui, Pei

    2016-05-01

    Lynch syndrome, an autosomal dominant inherited disorder, is caused by inactivating mutations involving DNA mismatch repair (MMR) genes. This leads to profound genetic instability, including microsatellite instability (MSI) and increased risk for cancer development, particularly colon and endometrial malignancies. Clinical testing of tumor tissues for the presence of MMR gene deficiency is standard practice in clinical oncology, with immunohistochemistry and PCR-based microsatellite instability analysis used as screening tests to identify potential Lynch syndrome families. The ultimate diagnosis of Lynch syndrome requires documentation of mutation within one of the four MMR genes (MLH1, PMS2, MSH2 and MSH6) or EPCAM, currently achieved by comprehensive sequencing analysis of germline DNA. In this review, the genetic basis of Lynch syndrome, methodologies of MMR deficiency testing, and current diagnostic algorithms in the clinical management of Lynch syndrome, are discussed. PMID:26895074

  20. A multivariate CAR model for mismatched lattices.

    PubMed

    Porter, Aaron T; Oleson, Jacob J

    2014-10-01

    In this paper, we develop a multivariate Gaussian conditional autoregressive model for use on mismatched lattices. Most current multivariate CAR models are designed for each multivariate outcome to utilize the same lattice structure. In many applications, a change of basis will allow different lattices to be utilized, but this is not always the case, because a change of basis is not always desirable or even possible. Our multivariate CAR model allows each outcome to have a different neighborhood structure which can utilize different lattices for each structure. The model is applied in two real data analysis. The first is a Bayesian learning example in mapping the 2006 Iowa Mumps epidemic, which demonstrates the importance of utilizing multiple channels of infection flow in mapping infectious diseases. The second is a multivariate analysis of poverty levels and educational attainment in the American Community Survey. PMID:25457598

  1. Global Health Governance and the Commercial Sector: A Documentary Analysis of Tobacco Company Strategies to Influence the WHO Framework Convention on Tobacco Control

    PubMed Central

    Weishaar, Heide; Collin, Jeff; Smith, Katherine; Grüning, Thilo; Mandal, Sema; Gilmore, Anna

    2012-01-01

    Background In successfully negotiating the Framework Convention on Tobacco Control (FCTC), the World Health Organization (WHO) has led a significant innovation in global health governance, helping to transform international tobacco control. This article provides the first comprehensive review of the diverse campaign initiated by transnational tobacco corporations (TTCs) to try to undermine the proposed convention. Methods and Findings The article is primarily based on an analysis of internal tobacco industry documents made public through litigation, triangulated with data from official documentation relating to the FCTC process and websites of relevant organisations. It is also informed by a comprehensive review of previous studies concerning tobacco industry efforts to influence the FCTC. The findings demonstrate that the industry's strategic response to the proposed WHO convention was two-fold. First, arguments and frames were developed to challenge the FCTC, including: claiming there would be damaging economic consequences; depicting tobacco control as an agenda promoted by high-income countries; alleging the treaty conflicted with trade agreements, “good governance,” and national sovereignty; questioning WHO's mandate; claiming the FCTC would set a precedent for issues beyond tobacco; and presenting corporate social responsibility (CSR) as an alternative. Second, multiple tactics were employed to promote and increase the impact of these arguments, including: directly targeting FCTC delegations and relevant political actors, enlisting diverse allies (e.g., mass media outlets and scientists), and using stakeholder consultation to delay decisions and secure industry participation. Conclusions TTCs' efforts to undermine the FCTC were comprehensive, demonstrating the global application of tactics that TTCs have previously been found to have employed nationally and further included arguments against the FCTC as a key initiative in global health governance

  2. Patient-Specific CT-Based Instrumentation versus Conventional Instrumentation in Total Knee Arthroplasty: A Prospective Randomized Controlled Study on Clinical Outcomes and In-Hospital Data

    PubMed Central

    Kotela, Andrzej; Lorkowski, Jacek; Kucharzewski, Marek; Wilk-Frańczuk, Magdalena; Śliwiński, Zbigniew; Frańczuk, Bogusław; Łęgosz, Paweł  ; Kotela, Ireneusz

    2015-01-01

    Total knee arthroplasty (TKA) is a frequently performed procedure in orthopaedic surgery. Recently, patient-specific instrumentation was introduced to facilitate correct positioning of implants. The aim of this study was to compare the early clinical results of TKA performed with patient-specific CT-based instrumentation and conventional technique. A prospective, randomized controlled trial on 112 patients was performed between January 2011 and December 2011. A group of 112 patients who met the inclusion and exclusion criteria were enrolled in this study and randomly assigned to an experimental or control group. The experimental group comprised 52 patients who received the Signature CT-based implant positioning system, and the control group consisted of 60 patients with conventional instrumentation. Clinical outcomes were evaluated with the KSS scale, WOMAC scale, and VAS scales to assess knee pain severity and patient satisfaction with the surgery. Specified in-hospital data were recorded. Patients were followed up for 12 months. At one year after surgery, there were no statistically significant differences between groups with respect to clinical outcomes and in-hospital data, including operative time, blood loss, hospital length of stay, intraoperative observations, and postoperative complications. Further high-quality investigations of various patient-specific systems and longer follow-up may be helpful in assessing their utility for TKA. PMID:26301241

  3. Evaluation of rotational control and forces generated during first-order archwire deflections: a comparison of self-ligating and conventional brackets.

    PubMed

    Pesce, Robert E; Uribe, Flavio; Janakiraman, Nandakumar; Neace, William P; Peterson, Donald R; Nanda, Ravindra

    2014-06-01

    The purpose of this study was to compare the activation and deactivation forces generated during first-order archwire deflections when different sizes and types of NiTi wires are paired with conventional and self-ligating brackets (SLBs) and to evaluate the rotational control between these same archwire and bracket combinations. Four maxillary premolar SLBs (Damon 3MX, SmartClip, Carriere, and In-Ovation R) and one conventional twin bracket (Victory) were paired with seven archwires [0.014, 0.016, 0.018, 0.016 × 0.022 Ultra Therm (thermal A f 80-90°F), 0.016, 0.018 SPEED Supercable, and 0.017 × 0.025 Turbo]. A cantilever test design was used and 10 trials per bracket/archwire combination were performed. Load/deflection data were captured over 4 mm fi rst-order archwire deflections. Forces generated were compared across all bracket/archwire combinations. Among thermal archwires, for a given deflection, forces increased with increasing archwire size. Supercable archwires displayed less force than their same size thermal counterparts. The Turbo archwire generated force values in between those of 0.016 and 0.018 thermal archwires. Rotational control improved with increasing wire dimensions and for a given archwire size. Rotational control among brackets generally ranked as follows: In-Ovation R > SmartClip > Carriere and Damon 3MX. PMID:22045693

  4. Mismatch Responses in the Awake Rat: Evidence from Epidural Recordings of Auditory Cortical Fields

    PubMed Central

    Jung, Fabienne; Stephan, Klaas Enno; Backes, Heiko; Moran, Rosalyn; Gramer, Markus; Kumagai, Tetsuya; Graf, Rudolf

    2013-01-01

    Detecting sudden environmental changes is crucial for the survival of humans and animals. In the human auditory system the mismatch negativity (MMN), a component of auditory evoked potentials (AEPs), reflects the violation of predictable stimulus regularities, established by the previous auditory sequence. Given the considerable potentiality of the MMN for clinical applications, establishing valid animal models that allow for detailed investigation of its neurophysiological mechanisms is important. Rodent studies, so far almost exclusively under anesthesia, have not provided decisive evidence whether an MMN analogue exists in rats. This may be due to several factors, including the effect of anesthesia. We therefore used epidural recordings in awake black hooded rats, from two auditory cortical areas in both hemispheres, and with bandpass filtered noise stimuli that were optimized in frequency and duration for eliciting MMN in rats. Using a classical oddball paradigm with frequency deviants, we detected mismatch responses at all four electrodes in primary and secondary auditory cortex, with morphological and functional properties similar to those known in humans, i.e., large amplitude biphasic differences that increased in amplitude with decreasing deviant probability. These mismatch responses significantly diminished in a control condition that removed the predictive context while controlling for presentation rate of the deviants. While our present study does not allow for disambiguating precisely the relative contribution of adaptation and prediction error processing to the observed mismatch responses, it demonstrates that MMN-like potentials can be obtained in awake and unrestrained rats. PMID:23646197

  5. Efficacy of adding the kinesio taping method to guideline-endorsed conventional physiotherapy in patients with chronic nonspecific low back pain: a randomised controlled trial

    PubMed Central

    2013-01-01

    Background Chronic nonspecific low back pain is a significant health condition with high prevalence worldwide and it is associated with enormous costs to society. Clinical practice guidelines show that many interventions are available to treat patients with chronic low back pain, but the vast majority of these interventions have a modest effect in reducing pain and disability. An intervention that has been widespread in recent years is the use of elastic bandages called Kinesio Taping. Although Kinesio Taping has been used extensively in clinical practice, current evidence does not support the use of this intervention; however these conclusions are based on a small number of underpowered studies. Therefore, questions remain about the effectiveness of the Kinesio Taping method as an additional treatment to interventions, such as conventional physiotherapy, that have already been recommended by the current clinical practice guidelines in robust and high-quality randomised controlled trials. We aim to determine the effectiveness of the addition of the use of Kinesio Taping in patients with chronic nonspecific low back pain who receive guideline-endorsed conventional physiotherapy. Methods/design One hundred and forty-eight patients will be randomly allocated to receive either conventional physiotherapy, which consists of a combination of manual therapy techniques, general exercises, and specific stabilisation exercises (Guideline-Endorsed Conventional Physiotherapy Group) or to receive conventional physiotherapy with the addition of Kinesio Taping to the lumbar spine (Conventional Physiotherapy plus Kinesio Taping Group) over a period of 5 weeks (10 sessions of treatment). Clinical outcomes (pain intensity, disability and global perceived effect) will be collected at baseline and at 5 weeks, 3 months, and 6 months after randomisation. We will also collect satisfaction with care and adverse effects after treatment. Data will be collected by a blinded assessor. All

  6. Linear-Optical Proposal for Implementation of the Two-Logic-Qubit Controlled Phase Gate in Decoherence-Free Subspace with Conventional Photon Detectors

    NASA Astrophysics Data System (ADS)

    Su, Shi-Lei; Guo, Qi; Wang, Hong-Fu; Zhang, Shou

    2016-01-01

    A linear-optical scheme is proposed to implement the nondeterministic two-qubit controlled phase gate in decoherence-free subspace (DFS) with a certain success probability. The proposed setup requires simple linear-optical elements, two pairs of the two-photon polarization entangled states as auxiliary resource, and the conventional photon detectors that only distinguish the vacuum and nonvacuum Fock number states. A sophisticated single-photon detector distinguishing one or two photon states is unnecessary. This makes the protocol more realizable in experiments.

  7. Randomized controlled clinical trial of oral health-related quality of life in patients wearing conventional and self-ligating brackets

    PubMed Central

    Mansor, Noorhanizar; Saub, Roslan

    2014-01-01

    Objective The aim of this randomized controlled clinical trial was to compare oral health-related quality of life (OHRQoL) of patients treated with conventional, active self-ligating (ASL), and passive self-ligating (PSL) brackets in different therapeutic phases. Methods Sixty patients (mean age 18.3 years; 29 males and 31 females) requiring orthodontic treatment were randomly and equally assigned to receive conventional (Victory Series), ASL (In-Ovation R), or PSL (Damon 3MX) brackets. OHRQoL was measured with a self-administered modified 16-item Malaysian version of the Oral Health Impact Profile for immediate (soon after the visit) and late (just before the subsequent visit) assessments of the bonding and activation phases. Data were analyzed with the Kruskal-Wallis and chi-square tests. Results The PSL and ASL groups showed more immediate and late impacts in the bonding phase, respectively; the conventional group was affected in both the assessments. The first activation phase had similar impacts in the groups. After the second activation, the conventional group showed more immediate impacts, whereas the PSL and ASL groups had more late impacts. The commonly affected domains were "physical disability," "functional limitation," "physical pain," and "psychological discomfort." No significant differences in the prevalence and severity of immediate and late impacts on OHRQoL of the patients were noted in any therapeutic phase. Conclusions No bracket system seems to ensure superior OHRQoL. This information could be useful for explaining the therapeutic phases, especially the initial one, and selecting the optimal bracket system based on the patient's preference. PMID:25133131

  8. Design and analysis of mismatch probes for long oligonucleotide microarrays

    SciTech Connect

    Deng, Ye; He, Zhili; Van Nostrand, Joy D.; Zhou, Jizhong

    2008-08-15

    Nonspecific hybridization is currently a major concern with microarray technology. One of most effective approaches to estimating nonspecific hybridizations in oligonucleotide microarrays is the utilization of mismatch probes; however, this approach has not been used for longer oligonucleotide probes. Here, an oligonucleotide microarray was constructed to evaluate and optimize parameters for 50-mer mismatch probe design. A perfect match (PM) and 28 mismatch (MM) probes were designed for each of ten target genes selected from three microorganisms. The microarrays were hybridized with synthesized complementary oligonucleotide targets at different temperatures (e.g., 42, 45 and 50 C). In general, the probes with evenly distributed mismatches were more distinguishable than those with randomly distributed mismatches. MM probes with 3, 4 and 5 mismatched nucleotides were differentiated for 50-mer oligonucleotide probes hybridized at 50, 45 and 42 C, respectively. Based on the experimental data generated from this study, a modified positional dependent nearest neighbor (MPDNN) model was constructed to adjust the thermodynamic parameters of matched and mismatched dimer nucleotides in the microarray environment. The MM probes with four flexible positional mismatches were designed using the newly established MPDNN model and the experimental results demonstrated that the redesigned MM probes could yield more consistent hybridizations. Conclusions: This study provides guidance on the design of MM probes for long oligonucleotides (e.g., 50 mers). The novel MPDNN model has improved the consistency for long MM probes, and this modeling method can potentially be used for the prediction of oligonucleotide microarray hybridizations.

  9. Effects of Mismatched Pictures on Retention of Illustrated Prose.

    ERIC Educational Resources Information Center

    Peeck, Joan

    A study was conducted to test the findings of two earlier studies (Peeck l974 and Pressley l983) on the effects of occasional mismatches between verbal and pictorial content in children's retention of illustrated prose. While the Peeck study indicated a considerable impact of mismatched pictures, the Pressley study indicated that with some…

  10. Educational Mismatch of Graduates: A Multidimensional and Fuzzy Indicator

    ERIC Educational Resources Information Center

    Betti, Gianni; D'Agostino, Antonella; Neri, Laura

    2011-01-01

    In this paper we attempt to measure the educational mismatch, seen as a problem of overeducation, using a multidimensional and fuzzy methodology. Educational mismatch can be difficult to measure because many factors can converge to its definition and the traditional unidimensional indicators presented in literature can offer a restricted view of…

  11. Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea

    PubMed Central

    Ishino, Sonoko; Nishi, Yuki; Oda, Soichiro; Uemori, Takashi; Sagara, Takehiro; Takatsu, Nariaki; Yamagami, Takeshi; Shirai, Tsuyoshi; Ishino, Yoshizumi

    2016-01-01

    The common mismatch repair system processed by MutS and MutL and their homologs was identified in Bacteria and Eukarya. However, no evidence of a functional MutS/L homolog has been reported for archaeal organisms, and it is not known whether the mismatch repair system is conserved in Archaea. Here, we describe an endonuclease that cleaves double-stranded DNA containing a mismatched base pair, from the hyperthermophilic archaeon Pyrococcus furiosus. The corresponding gene revealed that the activity originates from PF0012, and we named this enzyme Endonuclease MS (EndoMS) as the mismatch-specific Endonuclease. The sequence similarity suggested that EndoMS is the ortholog of NucS isolated from Pyrococcus abyssi, published previously. Biochemical characterizations of the EndoMS homolog from Thermococcus kodakarensis clearly showed that EndoMS specifically cleaves both strands of double-stranded DNA into 5′-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with a more preference for G/T, G/G and T/T, but has very little or no effect on C/C, A/C and A/A mismatches. The discovery of this endonuclease suggests the existence of a novel mismatch repair process, initiated by the double-strand break generated by the EndoMS endonuclease, in Archaea and some Bacteria. PMID:27001046

  12. Mechanisms in E. coli and Human Mismatch Repair (Nobel Lecture).

    PubMed

    Modrich, Paul

    2016-07-18

    DNA molecules are not completely stable, they are subject to chemical or photochemical damage and errors that occur during DNA replication resulting in mismatched base pairs. Through mechanistic studies Paul Modrich showed how replication errors are corrected by strand-directed mismatch repair in Escherichia coli and human cells. PMID:27198632

  13. Robust control for uncertain structures

    NASA Technical Reports Server (NTRS)

    Douglas, Joel; Athans, Michael

    1991-01-01

    Viewgraphs on robust control for uncertain structures are presented. Topics covered include: robust linear quadratic regulator (RLQR) formulas; mismatched LQR design; RLQR design; interpretations of RLQR design; disturbance rejection; and performance comparisons: RLQR vs. mismatched LQR.

  14. Effects of conventional versus multimodal vestibular rehabilitation on functional capacity and balance control in older people with chronic dizziness from vestibular disorders: design of a randomized clinical trial

    PubMed Central

    2012-01-01

    Background There are several protocols designed to treat vestibular disorders that focus on habituation, substitution, adaptation, and compensation exercises. However, protocols that contemplate not only vestibular stimulation but also other components that are essential to the body balance control in older people are rare. This study aims to compare the effectiveness of two vestibular rehabilitation protocols (conventional versus multimodal) on the functional capacity and body balance control of older people with chronic dizziness due to vestibular disorders. Methods/design A randomized, single-blind, controlled clinical trial with a 3 months follow-up period will be performed. The sample will be composed of older individuals with a clinical diagnosis of chronic dizziness resulting from vestibular disorders. The subjects will be evaluated at baseline, post-treatment and follow-up. Primary outcomes will be determined in accordance with the Dizziness Handicap Inventory (functional capacity) and the Dynamic Gait Index (body balance). Secondary outcomes include dizziness features, functional records, body balance control tests, and psychological information. The older individuals (minimum sample n = 68) will be randomized to either the conventional or multimodal Cawthorne&Cooksey protocols. The protocols will be performed during individual 50-minute sessions, twice a week, for 2 months (a total of 16 sessions). The outcomes of both protocols will be compared according to the intention-to-treat analysis. Discussion Vestibular rehabilitation through the Cawthorne&Cooksey protocol has already proved to be effective. However, the addition of other components related to body balance control has been proposed to improve the rehabilitation of older people with chronic dizziness from vestibular disorders. Trial registration ACTRN12610000018011 PMID:23276084

  15. Visual Mismatch Negativity Reveals Automatic Detection of Sequential Regularity Violation

    PubMed Central

    Stefanics, Gábor; Kimura, Motohiro; Czigler, István

    2011-01-01

    Sequential regularities are abstract rules based on repeating sequences of environmental events, which are useful to make predictions about future events. Here, we tested whether the visual system is capable to detect sequential regularity in unattended stimulus sequences. The visual mismatch negativity (vMMN) component of the event-related potentials is sensitive to the violation of complex regularities (e.g., object-related characteristics, temporal patterns). We used the vMMN component as an index of violation of conditional (if, then) regularities. In the first experiment, to investigate emergence of vMMN and other change-related activity to the violation of conditional rules, red and green disk patterns were delivered in pairs. The majority of pairs comprised of disk patterns with identical colors, whereas in deviant pairs the colors were different. The probabilities of the two colors were equal. The second member of the deviant pairs elicited a vMMN with longer latency and more extended spatial distribution to deviants with lower probability (10 vs. 30%). In the second (control) experiment the emergence of vMMN to violation of a simple, feature-related rule was studied using oddball sequences of stimulus pairs where deviant colors were presented with 20% probabilities. Deviant colored patterns elicited a vMMN, and this component was larger for the second member of the pair, i.e., after a shorter inter-stimulus interval. This result corresponds to the SOA/(v)MMN relationship, expected on the basis of a memory-mismatch process. Our results show that the system underlying vMMN is sensitive to abstract, conditional rules. Representation of such rules implicates expectation of a subsequent event, therefore vMMN can be considered as a correlate of violated predictions about the characteristics of environmental events. PMID:21629766

  16. Nucleotide metabolic mismatches in mammalian hearts: implications for transplantation

    PubMed Central

    Yacoub, MH; Smolenski, RT

    2013-01-01

    Introduction Human donor organ shortages have led surgeons and scientists to explore the use of animals as alternative organ sources. Acute thrombovascular rejection (AVR) is the main hurdle in xenotransplantation. Disparities in nucleotide metabolism in the vessels of different species may contribute significantly to the microvascular component of AVR. Methods We evaluated the extent of nucleotide metabolism mismatch in selected organs and endothelial cells of different mammals with particular focus on the changes in activity of ecto-5’-nucleotidase (E5’N) elicited by exposure of porcine hearts or endothelial cells to human blood (ex vivo) or human plasma (in vitro). Results E5’N activity in the rat heart was significantly higher than in other species. We noted a significant difference (p<0.001) in E5’N activity between human and pig endothelial cell lines. Initial pig aortic endothelial E5’N activity decreased in vitro after a three-hour exposure to human and porcine plasma while remaining constant in controls. Ex vivo perfusion with fresh human blood for four hours resulted in a significant decrease of E5’N activity in both wild type and transgenic pig hearts overexpressing human decay accelerating factor (p<0.001). Conclusions This study provides evidence that mismatches in basal mammalian metabolic pathways and humoral immunity interact in a xenogeneic environment. Understanding the role of nucleotide metabolism and signalling in xenotransplantation may identify new targets for genetic modifications and may lead to the development of new therapies extending graft survival. PMID:23317713

  17. Piezoelectric Versus Conventional Rotary Techniques for Impacted Third Molar Extraction: A Meta-analysis of Randomized Controlled Trials.

    PubMed

    Jiang, Qian; Qiu, Yating; Yang, Chi; Yang, Jingyun; Chen, Minjie; Zhang, Zhiyuan

    2015-10-01

    Impacted third molars are frequently encountered in clinical work. Surgical removal of impacted third molars is often required to prevent clinical symptoms. Traditional rotary cutting instruments are potentially injurious, and piezosurgery, as a new osteotomy technique, has been introduced in oral and maxillofacial surgery. No consistent conclusion has been reached regarding whether this new technique is associated with fewer or less severe postoperative sequelae after third molar extraction.The aim of this study was to compare piezosurgery with rotary osteotomy techniques, with regard to surgery time and the severity of postoperative sequelae, including pain, swelling, and trismus.We conducted a systematic literature search in the Cochrane Library, PubMed, Embase, and Google Scholar.The eligibility criteria of this study included the following: the patients were clearly diagnosed as having impacted mandibular third molars; the patients underwent piezosurgery osteotomy, and in the control group rotary osteotomy techniques, for removing impacted third molars; the outcomes of interest include surgery time, trismus, swelling or pain; the studies are randomized controlled trials.We used random-effects models to calculate the difference in the outcomes, and the corresponding 95% confidence interval. We calculated the weighted mean difference if the trials used the same measurement, and a standardized mean difference if otherwise.A total of seven studies met the eligibility criteria and were included in our analysis. Compared with rotary osteotomy, patients undergoing piezosurgery experienced longer surgery time (mean difference 4.13 minutes, 95% confidence interval 2.75-5.52, P < 0.0001). Patients receiving the piezoelectric technique had less swelling at postoperative days 1, 3, 5, and 7 (all Ps ≤0.023). Additionally, there was a trend of less postoperative pain and trismus in the piezosurgery groups.The number of included randomized controlled trials and the

  18. Comparison of Piezosurgery and Conventional Rotary Instruments for Removal of Impacted Mandibular Third Molars: A Randomized Controlled Clinical and Radiographic Trial

    PubMed Central

    Shokry, Mohamed; Aboelsaad, Nayer

    2016-01-01

    The purpose of this study was to test the effect of the surgical removal of impacted mandibular third molars using piezosurgery versus the conventional surgical technique on postoperative sequelae and bone healing. Material and Methods. This study was carried out as a randomized controlled clinical trial: split mouth design. Twenty patients with bilateral mandibular third molar mesioangular impaction class II position B indicated for surgical extraction were treated randomly using either the piezosurgery or the conventional bur technique on each site. Duration of the procedure, postoperative edema, trismus, pain, healing, and bone density and quantity were evaluated up to 6 months postoperatively. Results. Test and control sites were compared using paired t-test. There was statistical significance in reduction of pain and swelling in test sites, where the time of the procedure was statistically increased in test site. For bone quantity and quality, statistical difference was found where test site showed better results. Conclusion. Piezosurgery technique improves quality of patient's life in form of decrease of postoperative pain, trismus, and swelling. Furthermore, it enhances bone quality within the extraction socket and bone quantity along the distal aspect of the mandibular second molar. PMID:27597866

  19. Comparison of Piezosurgery and Conventional Rotary Instruments for Removal of Impacted Mandibular Third Molars: A Randomized Controlled Clinical and Radiographic Trial.

    PubMed

    Arakji, Hani; Shokry, Mohamed; Aboelsaad, Nayer

    2016-01-01

    The purpose of this study was to test the effect of the surgical removal of impacted mandibular third molars using piezosurgery versus the conventional surgical technique on postoperative sequelae and bone healing. Material and Methods. This study was carried out as a randomized controlled clinical trial: split mouth design. Twenty patients with bilateral mandibular third molar mesioangular impaction class II position B indicated for surgical extraction were treated randomly using either the piezosurgery or the conventional bur technique on each site. Duration of the procedure, postoperative edema, trismus, pain, healing, and bone density and quantity were evaluated up to 6 months postoperatively. Results. Test and control sites were compared using paired t-test. There was statistical significance in reduction of pain and swelling in test sites, where the time of the procedure was statistically increased in test site. For bone quantity and quality, statistical difference was found where test site showed better results. Conclusion. Piezosurgery technique improves quality of patient's life in form of decrease of postoperative pain, trismus, and swelling. Furthermore, it enhances bone quality within the extraction socket and bone quantity along the distal aspect of the mandibular second molar. PMID:27597866

  20. Targeting intensive versus conventional glycaemic control for type 1 diabetes mellitus: a systematic review with meta-analyses and trial sequential analyses of randomised clinical trials

    PubMed Central

    Kähler, Pernille; Grevstad, Berit; Almdal, Thomas; Gluud, Christian; Wetterslev, Jørn; Vaag, Allan; Hemmingsen, Bianca

    2014-01-01

    Objective To assess the benefits and harms of targeting intensive versus conventional glycaemic control in patients with type 1 diabetes mellitus. Design A systematic review with meta-analyses and trial sequential analyses of randomised clinical trials. Data sources The Cochrane Library, MEDLINE, EMBASE, Science Citation Index Expanded and LILACS to January 2013. Study selection Randomised clinical trials that prespecified different targets of glycaemic control in participants at any age with type 1 diabetes mellitus were included. Data extraction Two authors independently assessed studies for inclusion and extracted data. Results 18 randomised clinical trials included 2254 participants with type 1 diabetes mellitus. All trials had high risk of bias. There was no statistically significant effect of targeting intensive glycaemic control on all-cause mortality (risk ratio 1.16, 95% CI 0.65 to 2.08) or cardiovascular mortality (0.49, 0.19 to 1.24). Targeting intensive glycaemic control reduced the relative risks for the composite macrovascular outcome (0.63, 0.41 to 0.96; p=0.03), and nephropathy (0.37, 0.27 to 0.50; p<0.00001. The effect estimates of retinopathy, ketoacidosis and retinal photocoagulation were not consistently statistically significant between random and fixed effects models. The risk of severe hypoglycaemia was significantly increased with intensive glycaemic targets (1.40, 1.01 to 1.94). Trial sequential analyses showed that the amount of data needed to demonstrate a relative risk reduction of 10% were, in general, inadequate. Conclusions There was no significant effect towards improved all-cause mortality when targeting intensive glycaemic control compared with conventional glycaemic control. However, there may be beneficial effects of targeting intensive glycaemic control on the composite macrovascular outcome and on nephropathy, and detrimental effects on severe hypoglycaemia. Notably, the data for retinopathy and ketoacidosis were inconsistent

  1. Determination of value of bovine respiratory disease control using a remote early disease identification system compared with conventional methods of metaphylaxis and visual observations.

    PubMed

    White, B J; Amrine, D E; Goehl, D R

    2015-08-01

    Mitigation of the deleterious effects of bovine respiratory disease (BRD) is an important issue in the cattle industry. Conventional management of calves at high risk for BRD often includes mass treatment with antimicrobials at arrival followed by visual observation for individual clinical cases. These methods have proven effective; however, control program efficacy is influenced by the accuracy of visual observation. A remote early disease identification (REDI) system has been described that monitors cattle behavior to identify potential BRD cases. The objective of this research was to compare health and performance outcomes using either traditional BRD control (visual observation and metaphylaxis) or REDI during a 60-d postarrival phase in high-risk beef calves. The randomized controlled clinical trial was performed in 8 replicates at 3 different facilities over a 19-mo period. In each replicate, a single load of calves was randomly allocated to receive either conventional management (CONV; total = 8) or REDI (total = 8) as the method for BRD control. Cattle were monitored with each diagnostic method for the first 30 d on feed and performance variables were collected until approximately 60 d after arrival. Statistical differences ( < 0.10) were not identified in common performance (ADG) or health (morbidity, first treatment success, and mortality risk) among the treatment groups. Calves in the REDI pens had a lower ( < 0.01) average number of days on feed at first treatment (9.1 ± 1.2 d) compared with CONV pens (15.8 ± 1.2 d). There were no statistical differences ( > 0.10) in risk of BRD treatment and REDI calves were not administered antimicrobials at arrival; therefore, REDI calves had a lower ( < 0.01) average number of doses of antimicrobials/calf (0.75 ± 0.1 doses) compared with CONV calves (1.67 ± 0.1 doses). In this trial, the REDI system was comparable to conventional management with the potential advantages of earlier BRD diagnosis and decreased use

  2. Mammalian cells defective in DNA mismatch correction

    SciTech Connect

    Branch, P.; Aquilina, G.; Hess, P.

    1994-12-31

    Mammalian cells counteract the cytotoxicity of methylating agents, including some used in antitumor chemotherapy, by removing the methylated base, O{sup 6}-methylguanine (O{sup 6}-meG) from their DNA. This removal is normally effected by a specific DNA repair enzyme (O{sup 6}-meG-DNA methyltransferase) that is expressed constitutively. In addition, an alternative type of resistance to methylating agents can be acquired after exposure of cells to the drug. This acquired resistance is highly specific for O{sup 6}-meG and is unusual in that alkylation of DNA is normal and there is no increase in the rate of repair of O{sup 6}-meG or any other damaged base. Instead, the cell is able to tolerate the presence of the usually cytotoxic O{sup 6}-meG and to replicate its DNA normally. The ambiguity of base pairing by O{sup 6}-meG and the observation that tolerant cells are also cross-resistant to the structurally similar 6-thioguanine in DNA has led to the suggestion that the cytotoxicity of O{sup 6}-meG (and 6-thioguanine) arises from ineffective attempts at DNA mismatch correction. This model postulates that tolerance arises as a consequence of loss of this important pathway.

  3. The effects of phenological mismatches on demography

    PubMed Central

    Miller-Rushing, Abraham J.; Høye, Toke Thomas; Inouye, David W.; Post, Eric

    2010-01-01

    Climate change is altering the phenology of species across the world, but what are the consequences of these phenological changes for the demography and population dynamics of species? Time-sensitive relationships, such as migration, breeding and predation, may be disrupted or altered, which may in turn alter the rates of reproduction and survival, leading some populations to decline and others to increase in abundance. However, finding evidence for disrupted relationships, or lack thereof, and their demographic effects, is difficult because the necessary detailed observational data are rare. Moreover, we do not know how sensitive species will generally be to phenological mismatches when they occur. Existing long-term studies provide preliminary data for analysing the phenology and demography of species in several locations. In many instances, though, observational protocols may need to be optimized to characterize timing-based multi-trophic interactions. As a basis for future research, we outline some of the key questions and approaches to improving our understanding of the relationships among phenology, demography and climate in a multi-trophic context. There are many challenges associated with this line of research, not the least of which is the need for detailed, long-term data on many organisms in a single system. However, we identify key questions that can be addressed with data that already exist and propose approaches that could guide future research. PMID:20819811

  4. Influence of framework design, contraction mismatch, and thermal history on porcelain checking in fixed partial dentures.

    PubMed

    Anusavice, K J; Gray, A E

    1989-01-01

    The objective of this study was to characterize the relative influence of contraction mismatch, framework design, furnace type, cooling rate, and multiple firings on immediate or delayed checking in fixed partial dentures. Frameworks for 60 anterior bridges (three-unit fixed partial dentures) were cast from a low-expansion Au-Pd alloy (O) and a high-expansion Pd-Ag alloy (J). A high-expansion porcelain (B) was applied to each of three framework designs. Firing was performed at heating rates of 56 degrees C/min and 180 degrees C/min. Specimens were cooled at two rates after each of five glazing cycles. For O-B specimens which exhibited a negative thermal contraction mismatch between 600 degrees C and 25 degrees C, 60% of the bridge specimens failed when they were subjected to slow cooling preceded by either fast or slow heating. When J-B specimens (which exhibited a smaller negative contraction mismatch) were heated and cooled rapidly, no failures occurred through all of the firing cycles. However, cracks were observed in 13.3% of the J-B bridges which were slowly heated and rapidly cooled. Delayed cracks (after the fifth glaze cycle) developed over periods of up to two years only in bridges which were slowly cooled in the furnace chamber. The results of this study suggest that checking in conventional feldspathic porcelains can be promoted by slow cooling rates and an excessive number of firing cycles. PMID:2691298

  5. Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea.

    PubMed

    Ishino, Sonoko; Nishi, Yuki; Oda, Soichiro; Uemori, Takashi; Sagara, Takehiro; Takatsu, Nariaki; Yamagami, Takeshi; Shirai, Tsuyoshi; Ishino, Yoshizumi

    2016-04-20

    The common mismatch repair system processed by MutS and MutL and their homologs was identified in Bacteria and Eukarya. However, no evidence of a functional MutS/L homolog has been reported for archaeal organisms, and it is not known whether the mismatch repair system is conserved in Archaea. Here, we describe an endonuclease that cleaves double-stranded DNA containing a mismatched base pair, from the hyperthermophilic archaeonPyrococcus furiosus The corresponding gene revealed that the activity originates from PF0012, and we named this enzyme Endonuclease MS (EndoMS) as themismatch-specific Endonuclease. The sequence similarity suggested that EndoMS is the ortholog of NucS isolated fromPyrococcus abyssi, published previously. Biochemical characterizations of the EndoMS homolog fromThermococcus kodakarensisclearly showed that EndoMS specifically cleaves both strands of double-stranded DNA into 5'-protruding forms, with the mismatched base pair in the central position. EndoMS cleaves G/T, G/G, T/T, T/C and A/G mismatches, with a more preference for G/T, G/G and T/T, but has very little or no effect on C/C, A/C and A/A mismatches. The discovery of this endonuclease suggests the existence of a novel mismatch repair process, initiated by the double-strand break generated by the EndoMS endonuclease, in Archaea and some Bacteria. PMID:27001046

  6. Heterogenous mismatch-repair status in colorectal cancer

    PubMed Central

    2014-01-01

    Abstract Background Immunohistochemical staining for mismatch repair proteins is efficient and widely used to identify mismatch repair defective tumors. The tumors typically show uniform and widespread loss of MMR protein staining. We identified and characterized colorectal cancers with alternative, heterogenous mismatch repair protein staining in order to delineate expression patterns and underlying mechanisms. Methods Heterogenous staining patterns that affected at least one of the mismatch repair proteins MLH1, PMS2, MSH2 and MSH6 were identified in 14 colorectal cancers. Based on alternative expression patterns macro-dissected and micro-dissected tumor areas were separately analyzed for microsatellite instability and MLH1 promoter methylation. Results Heterogenous retained/lost mismatch repair protein expression could be classified as intraglandular (within or in-between glandular formations), clonal (in whole glands or groups of glands) and compartmental (in larger tumor areas/compartments or in between different tumor blocks). These patterns coexisted in 9/14 tumors and in the majority of the tumors correlated with differences in microsatellite instability/MLH1 methylation status. Conclusions Heterogenous mismatch repair status can be demonstrated in colorectal cancer. Though rare, attention to this phenomenon is recommended since it corresponds to differences in mismatch repair status that are relevant for correct classification. Virtual Slides The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1771940323126788 PMID:24968821

  7. Effects of an integrated Yoga Program on Self-reported Depression Scores in Breast Cancer Patients Undergoing Conventional Treatment: A Randomized Controlled Trial

    PubMed Central

    Rao, Raghavendra Mohan; Raghuram, Nagarathna; Nagendra, HR; Usharani, MR; Gopinath, KS; Diwakar, Ravi B; Patil, Shekar; Bilimagga, Ramesh S; Rao, Nalini

    2015-01-01

    Aim: To compare the effects of yoga program with supportive therapy on self-reported symptoms of depression in breast cancer patients undergoing conventional treatment. Patients and Methods: Ninety-eight breast cancer patients with stage II and III disease from a cancer center were randomly assigned to receive yoga (n = 45) and supportive therapy (n = 53) over a 24-week period during which they underwent surgery followed by adjuvant radiotherapy (RT) or chemotherapy (CT) or both. The study stoppage criteria was progressive disease rendering the patient bedridden or any physical musculoskeletal injury resulting from intervention or less than 60% attendance to yoga intervention. Subjects underwent yoga intervention for 60 min daily with control group undergoing supportive therapy during their hospital visits. Beck's Depression Inventory (BDI) and symptom checklist were assessed at baseline, after surgery, before, during, and after RT and six cycles of CT. We used analysis of covariance (intent-to-treat) to study the effects of intervention on depression scores and Pearson correlation analyses to evaluate the bivariate relationships. Results: A total of 69 participants contributed data to the current analysis (yoga, n = 33, and controls, n = 36). There was 29% attrition in this study. The results suggest an overall decrease in self-reported depression with time in both the groups. There was a significant decrease in depression scores in the yoga group as compared to controls following surgery, RT, and CT (P < 0.01). There was a positive correlation (P < 0.001) between depression scores with symptom severity and distress during surgery, RT, and CT. Conclusion: The results suggest possible antidepressant effects with yoga intervention in breast cancer patients undergoing conventional treatment. PMID:26009671

  8. Modeling of integrated environmental control systems for coal-fired power plants: Conventional froth flotation for the IEC coal cleaning plant model

    SciTech Connect

    Rubin, E.S.

    1989-01-01

    This report describes the addition of a conventional froth flotation circuit into the FORTRAN coal cleaning module of the Integrated Environmental Control (IEC) model. The purpose of this modification is to include froth flotation as an option to clean the coal fines. The current model has three beneficiation: levels (2, 3, and 4) in which different streams are washed by specific gravity equipment. Level 2 washes only the coarse stream. Level 3 washes the coarse and medium streams. Level 4 washes the coarse, medium, and fine streams. This modification adds a fifth level, which uses specific gravity equipment to wash the coarse and medium streams and froth flotation equipment for the fine stream. The specific size fractions in each stream are specified by the model user. As before, the model optimizes the yield of each circuit in order to achieve a target coal quality for the cleaned coal product.

  9. Effectiveness of percutaneous laser disc decompression versus conventional open discectomy in the treatment of lumbar disc herniation; design of a prospective randomized controlled trial

    PubMed Central

    Brouwer, Patrick A; Peul, Wilco C; Brand, Ronald; Arts, Mark P; Koes, Bart W; Berg, Annette A van den; van Buchem, Mark A

    2009-01-01

    Background The usual surgical treatment of refractory sciatica caused by lumbar disc herniation, is open discectomy. Minimally invasive procedures, including percutaneous therapies under local anesthesia, are increasingly gaining attention. One of these treatments is Percutaneous Laser Disc Decompression (PLDD). This treatment can be carried out in an outpatient setting and swift recovery and return to daily routine are suggested. Thus far, no randomized trial into cost-effectiveness of PLDD versus standard surgical procedure has been performed. We present the design of a randomized controlled trial, studying the cost-effectiveness of PLDD versus conventional open discectomy in patients with sciatica from lumbar disc herniation. Methods/design The study is a randomized prospective multi-center trial, in which two treatment strategies are compared in a parallel group design. Patients (age 18–70 years) visiting the neurosurgery department of the participating hospitals, are considered for inclusion in the trial when sciatica due to lumbar disc herniation has lasted more than 8 weeks. Patients with disc herniation smaller than 1/3 of the spinal canal diameter, without concomitant lateral recess stenosis or sequestration, are eligible for participation, and are randomized into one of two treatment arms; either Percutaneous Laser Disc Decompression or conventional discectomy. The functional outcome of the patient, as assessed by the Roland Disability Questionnaire for Sciatica at 8 weeks and 1 year after treatment, is the primary outcome measure. The secondary outcome parameters are recovery as perceived by the patient, leg and back pain, incidence of re-intervention, complications, quality of life, medical consumption, absence of work and secondary costs. Discussion Open discectomy is still considered to be the golden standard in the surgical treatment of lumbar disc herniation. Whether Percutaneous Laser Disc Decompression has at least as much efficacy as the

  10. Policy makers’ viewpoints on implementation of the World Health Organization Framework Convention on Tobacco Control in Iran: A qualitative investigation of program facilitators

    PubMed Central

    Sarrafzadegan, Nizal; Rabiei, Katayoun; Alavi, Mousa; Kelishadi, Roya; Manzouri, Leila; Abedi, Heidarali; Fereydoun-Mohaseli, Khadijeh; Azaripour-Masooleh, Hasan; Roohafza, Hamidreza; Heidari, Gholamreza

    2016-01-01

    BACKGROUND The epidemic of smoking is a great concern of health systems. Moreover, the number of smokers is increasing worldwide and this has led to an escalating trend of morbidity, mortality, and burden of smoking-related diseases. Therefore, monitoring the implementation of tobacco control laws in different countries is of extreme importance. This study aimed to describe policy makers’ experiences and perceptions of the facilitating factors of the implementation of the World Health Organization Framework Convention on Tobacco Control (WHO FCTC) in Iran. METHODS This was a qualitative research in which data were collected through individual interviews. The participants included policy makers who were members of the national assembly for tobacco control. In this study, 13 unstructured interviews of about 45 to 60 minutes duration were conducted in an extrapolative manner. The qualitative content analysis method was applied until extrapolation of basic themes was complete. RESULTS As a result of the analysis, the themes of performance through training, through research, through intersectoral collaboration, and through setting priorities emerged. The emerged themes connote some critical points that have key roles in promoting the effective implementation of the WHO FCTC. Furthermore, the main role of the health sector becomes predominant. CONCLUSION The study findings suggested the managed and coordinated work as one of the main facilitating factors of the implementation of the WHO FCTC at a national level. PMID:27429629

  11. [Effects of applying controlled-release fertilizer blended with conventional nitrogen fertilizer on Chinese cabbage yield and quality as well as nitrogen losses].

    PubMed

    Yang, Jun-gang; Xu, Kai; Tong, Er-jian; Cao, Bing; Ni, Xiao-hui; Xu, Jun-xiang

    2010-12-01

    An open field experiment was conducted to study the effects of applying controlled-release fertilizer blended with rapidly available chemical N fertilizer on Chinese cabbage yield and quality as well as nitrogen losses, including ammonia volatilization and NO3- -N accumulation and leaching in Beijing suburb. The results showed that a combined application of 2:1 controlled-release fertilizer and urea fertilizer (total N rate 150 kg x hm(-2)) did not induce the reduction of Chinese cabbage yield, and decreased the leaf nitrate and organic acid contents significantly, compared with conventional urea N application (300 kg x hm(-2)), and had no significant difference in the cabbage yield and leaf nitrate content, compared with applying 150 kg x hm(-2) of urea N. The combined application of 2:1 controlled-release fertilizer and urea fertilizer improved the N use efficiency of Chinese cabbage, and reduced the ammonia volatilization and NO3- -N leaching. At harvest, the NO3- -N concentrations in 20-40, 60-80 and 80-100 cm soil layers were significantly lower in the combined application treatment than in urea N treatment. PMID:21443002

  12. Polarizing keys prevent mismatch of connector plugs and receptacles

    NASA Technical Reports Server (NTRS)

    Chiapuzio, A.

    1966-01-01

    Keying prevents mismatching of plugs and receptacles in connector patching of instrumentation involving several thousand leads. Each receptacle and plug contains three polarizing keys that must mate in a complementary mode before the connector pins and sockets will engage.

  13. Heterodyne detection with mismatch correction based on array detector

    NASA Astrophysics Data System (ADS)

    Dong, Hongzhou; Li, Guoqiang; Yang, Ruofu; Yang, Chunping; Ao, Mingwu

    2016-07-01

    Based on an array detector, a new heterodyne detection system, which can correct the mismatches of amplitude and phase between signal and local oscillation (LO) beams, is presented in this paper. In the light of the fact that, for a heterodyne signal, there is a certain phase difference between the adjacent two samples of analog-to-digital converter (ADC), we propose to correct the spatial phase mismatch by use of the time-domain phase difference. The corrections can be realized by shifting the output sequences acquired from the detector elements in the array, and the steps of the shifting depend on the quantity of spatial phase mismatch. Numerical calculations of heterodyne efficiency are conducted to confirm the excellent performance of our system. Being different from previous works, our system needs not extra optical devices, so it provides probably an effective means to ease the problem resulted from the mismatches.

  14. Heterodyne detection with mismatch correction base on array detector

    NASA Astrophysics Data System (ADS)

    Hongzhou, Dong; Guoqiang, Li; Ruofu, Yang; Chunping, Yang; Mingwu, Ao

    2016-07-01

    Based on an array detector, a new heterodyne detection system, which can correct the mismatches of amplitude and phase between signal and local oscillation (LO) beams, is presented in this paper. In the light of the fact that, for a heterodyne signal, there is a certain phase difference between the adjacent two samples of analog-to-digital converter (ADC), we propose to correct the spatial phase mismatch by use of the time-domain phase difference. The corrections can be realized by shifting the output sequences acquired from the detector elements in the array, and the steps of the shifting depend on the quantity of spatial phase mismatch. Numerical calculations of heterodyne efficiency are conducted to confirm the excellent performance of our system. Being different from previous works, our system needs not extra optical devices, so it provides probably an effective means to ease the problem resulted from the mismatches.

  15. Elastic-plastic fracture mechanics of strength-mismatching

    SciTech Connect

    Parks, D.M.; Ganti, S.; McClintock, F.A.

    1996-12-31

    Approximate solutions to stress-fields are provided for a strength-mismatched interface crack in small-scale yielding (SSY) for non-hardening and low hardening materials. Variations of local deformation intensities, characterized by a J-type contour integral, are proposed. The softer material experiences a higher deformation intensity level, J{sub S}, while the harder material sees a much lower deformation intensity level, J{sub H}, compared to that obtained from the applied J near the respective homogeneous crack-tips. For a low hardening material, the stress fields are obtained by scaling from an elastic/perfectly-plastic problem, based on an effective mismatch, M{sub eff}, which is a function of mismatch, M, and the hardening exponent, n. Triaxial stress build-up is discussed quantitatively in terms of M. The influence of strength-mismatch on cleavage fracture is discussed using Weibull statistics.

  16. Animal Assisted Therapy (AAT) Program As a Useful Adjunct to Conventional Psychosocial Rehabilitation for Patients with Schizophrenia: Results of a Small-scale Randomized Controlled Trial

    PubMed Central

    Calvo, Paula; Fortuny, Joan R.; Guzmán, Sergio; Macías, Cristina; Bowen, Jonathan; García, María L.; Orejas, Olivia; Molins, Ferran; Tvarijonaviciute, Asta; Cerón, José J.; Bulbena, Antoni; Fatjó, Jaume

    2016-01-01

    Currently, one of the main objectives of human–animal interaction research is to demonstrate the benefits of animal assisted therapy (AAT) for specific profiles of patients or participants. The aim of this study is to assess the effect of an AAT program as an adjunct to a conventional 6–month psychosocial rehabilitation program for people with schizophrenia. Our hypothesis is that the inclusion of AAT into psychosocial rehabilitation would contribute positively to the impact of the overall program on symptomology and quality of life, and that AAT would be a positive experience for patients. To test these hypotheses, we compared pre–program with post–program scores for the Positive and Negative Syndrome Scale (PANSS) and the EuroQoL-5 dimensions questionnaire (EuroQol-5D), pre–session with post–session salivary cortisol and alpha–amylase for the last four AAT sessions, and adherence rates between different elements of the program. We conducted a randomized, controlled study in a psychiatric care center in Spain. Twenty–two institutionalized patients with chronic schizophrenia completed the 6–month rehabilitation program, which included individual psychotherapy, group therapy, a functional program (intended to improve daily functioning), a community program (intended to facilitate community reintegration) and a family program. Each member of the control group (n = 8) participated in one activity from a range of therapeutic activities that were part of the functional program. In place of this functional program activity, the AAT–treatment group (n = 14) participated in twice–weekly 1–h sessions of AAT. All participants received the same weekly total number of hours of rehabilitation. At the end of the program, both groups (control and AAT–treatment) showed significant improvements in positive and overall symptomatology, as measured with PANSS, but only the AAT–treatment group showed a significant improvement in negative symptomatology

  17. Animal Assisted Therapy (AAT) Program As a Useful Adjunct to Conventional Psychosocial Rehabilitation for Patients with Schizophrenia: Results of a Small-scale Randomized Controlled Trial.

    PubMed

    Calvo, Paula; Fortuny, Joan R; Guzmán, Sergio; Macías, Cristina; Bowen, Jonathan; García, María L; Orejas, Olivia; Molins, Ferran; Tvarijonaviciute, Asta; Cerón, José J; Bulbena, Antoni; Fatjó, Jaume

    2016-01-01

    Currently, one of the main objectives of human-animal interaction research is to demonstrate the benefits of animal assisted therapy (AAT) for specific profiles of patients or participants. The aim of this study is to assess the effect of an AAT program as an adjunct to a conventional 6-month psychosocial rehabilitation program for people with schizophrenia. Our hypothesis is that the inclusion of AAT into psychosocial rehabilitation would contribute positively to the impact of the overall program on symptomology and quality of life, and that AAT would be a positive experience for patients. To test these hypotheses, we compared pre-program with post-program scores for the Positive and Negative Syndrome Scale (PANSS) and the EuroQoL-5 dimensions questionnaire (EuroQol-5D), pre-session with post-session salivary cortisol and alpha-amylase for the last four AAT sessions, and adherence rates between different elements of the program. We conducted a randomized, controlled study in a psychiatric care center in Spain. Twenty-two institutionalized patients with chronic schizophrenia completed the 6-month rehabilitation program, which included individual psychotherapy, group therapy, a functional program (intended to improve daily functioning), a community program (intended to facilitate community reintegration) and a family program. Each member of the control group (n = 8) participated in one activity from a range of therapeutic activities that were part of the functional program. In place of this functional program activity, the AAT-treatment group (n = 14) participated in twice-weekly 1-h sessions of AAT. All participants received the same weekly total number of hours of rehabilitation. At the end of the program, both groups (control and AAT-treatment) showed significant improvements in positive and overall symptomatology, as measured with PANSS, but only the AAT-treatment group showed a significant improvement in negative symptomatology. Adherence to the AAT

  18. Interaction between Mismatch Repair and Genetic Recombination in Saccharomyces Cerevisiae

    PubMed Central

    Alani, E.; Reenan, RAG.; Kolodner, R. D.

    1994-01-01

    The yeast Saccharomyces cerevisiae encodes a set of genes that show strong amino acid sequence similarity to MutS and MutL, proteins required for mismatch repair in Escherichia coli. We examined the role of MSH2 and PMS1, yeast homologs of mutS and mutL, respectively, in the repair of base pair mismatches formed during meiotic recombination. By using specifically marked HIS4 and ARG4 alleles, we showed that msh2 mutants displayed a severe defect in the repair of all base pair mismatches as well as 1-, 2- and 4-bp insertion/deletion mispairs. The msh2 and pms1 phenotypes were indistinguishable, suggesting that the wild-type gene products act in the same repair pathway. A comparison of gene conversion events in wild-type and msh2 mutants indicated that mismatch repair plays an important role in genetic recombination. (1) Tetrad analysis at five different loci revealed that, in msh2 mutants, the majority of aberrant segregants displayed a sectored phenotype, consistent with a failure to repair mismatches created during heteroduplex formation. In wild type, base pair mismatches were almost exclusively repaired toward conversion rather than restoration. (2) In msh2 strains 10-19% of the aberrant tetrads were Ab4:4. (3) Polarity gradients at HIS4 and ARG4 were nearly abolished in msh2 mutants. The frequency of gene conversion at the 3' end of these genes was increased and was nearly the frequency observed at the 5' end. (4) Co-conversion studies were consistent with mismatch repair acting to regulate heteroduplex DNA tract length. We favor a model proposing that recombination events occur through the formation and resolution of heteroduplex intermediates and that mismatch repair proteins specifically interact with recombination enzymes to regulate the length of symmetric heteroduplex DNA. PMID:8056309

  19. Penumbra detection using PWI/DWI mismatch MRI in a rat stroke model with and without comorbidity: comparison of methods

    PubMed Central

    Reid, Emma; Graham, Delyth; Lopez-Gonzalez, M Rosario; Holmes, William M; Macrae, I Mhairi; McCabe, Christopher

    2012-01-01

    Perfusion-diffusion (perfusion-weighted imaging (PWI)/diffusion-weighted imaging (DWI)) mismatch is used to identify penumbra in acute stroke. However, limitations in penumbra detection with mismatch are recognized, with a lack of consensus on thresholds, quantification and validation of mismatch. We determined perfusion and diffusion thresholds from final infarct in the clinically relevant spontaneously hypertensive stroke-prone (SHRSP) rat and its normotensive control strain, Wistar-Kyoto (WKY) and compared three methods for penumbra calculation. After permanent middle cerebral artery occlusion (MCAO) (WKY n=12, SHRSP n=15), diffusion-weighted (DWI) and perfusion-weighted (PWI) images were obtained for 4 hours post stroke and final infarct determined at 24 hours on T2 scans. The PWI/DWI mismatch was calculated from volumetric assessment (perfusion deficit volume minus apparent diffusion coefficient (ADC)-defined lesion volume) or spatial assessment of mismatch area on each coronal slice. The ADC-derived lesion growth provided the third, retrospective measure of penumbra. At 1 hour after MCAO, volumetric mismatch detected smaller volumes of penumbra in both strains (SHRSP: 31±50 mm3, WKY: 22±59 mm3, mean±s.d.) compared with spatial assessment (SHRSP: 36±15 mm3, WKY: 43±43 mm3) and ADC lesion expansion (SHRSP: 41±45 mm3, WKY: 65±41 mm3), although these differences were not statistically significant. Spatial assessment appears most informative, using both diffusion and perfusion data, eliminating the influence of negative mismatch and allowing the anatomical location of penumbra to be assessed at given time points after stroke. PMID:22669479

  20. The Effect of Basepair Mismatch on DNA Strand Displacement.

    PubMed

    Broadwater, D W Bo; Kim, Harold D

    2016-04-12

    DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized. Here, we measured toehold-mediated strand displacement kinetics using single-molecule fluorescence in the presence of a single basepair mismatch. The apparent displacement rate varied significantly when the mismatch was introduced in the invading DNA strand. The rate generally decreased as the mismatch in the invader was encountered earlier in displacement. Our data indicate that a single base pair mismatch in the invader stalls branch migration and displacement occurs via direct dissociation of the destabilized incumbent strand from the substrate strand. We combined both branch migration and direct dissociation into a model, which we term the concurrent displacement model, and used the first passage time approach to quantitatively explain the salient features of the observed relationship. We also introduce the concept of splitting probabilities to justify that the concurrent model can be simplified into a three-step sequential model in the presence of an invader mismatch. We expect our model to become a powerful tool to design DNA-based reaction schemes with broad functionality. PMID:27074674

  1. Stress-controlled Pb(Zr0.52Ti0.48)O3 thick films by thermal expansion mismatch between substrate and Pb(Zr0.52Ti0.48)O3 film

    NASA Astrophysics Data System (ADS)

    Han, Guifang; Ryu, Jungho; Yoon, Woon-Ha; Choi, Jong-Jin; Hahn, Byung-Dong; Kim, Jong-Woo; Park, Dong-Soo; Ahn, Cheol-Woo; Priya, Shashank; Jeong, Dae-Yong

    2011-12-01

    Polycrystalline Pb(Zr0.52Ti0.48)O3 (PZT) thick films (thickness ˜10 μm) were successfully fabricated by using a novel aerosol deposition technique on Si wafer, sapphire, and single crystal yitria stabilized zirconia (YSZ) wafer substrates with Pt electrodes and their dielectric, ferroelectric, and piezoelectric properties, and in-plane stresses were investigated. The films with different stress conditions were simply controlled by the coefficient of thermal expansion (CTE) misfit of PZT films and substrates. The results showed that the films bearing in-plane compressive stress deposited on the YSZ and sapphire substrates have superior dielectric, ferroelectric (˜90%), and piezoelectric (>200%) properties over that of the Si wafer. Among these three substrates, YSZ shows superior properties of the PZT films. However, films on Si wafer with tensile stress present lower properties. We believed that in-plane compressive stresses within the films are benefited, the formation of c-domain parallel to the thickness direction resulting in the higher piezoelectric properties. These results suggest that the properties of polycrystalline PZT thick films can be adjusted by simply choosing the substrates with different CTEs.

  2. Conventional Risk Factors and Acute Coronary Syndrome during a Period of Socioeconomic Transition: Population-based Case-control Study in Tirana, Albania

    PubMed Central

    Burazeri, Genc; Goda, Artan; Sulo, Gerhard; Stefa, Jonida; Roshi, Enver; Kark, Jeremy D.

    2007-01-01

    Aim To assess the association between conventional risk factors and acute coronary syndrome in Albania, a transitional country in Southeast Europe. Methods A population-based case-control study was conducted in Tirana in 2003-2006. A total of 467 consecutive patients with nonfatal acute coronary syndrome were recruited. There were 370 men with mean ± standard deviation (SD) age of 59.1 ± 8.7 years and 97 women with mean±SD age of 63.3 ± 7.1 years. The control group comprised a population-representative sample of Tirana residents. In the control group, there were 469 men with mean±SD age of 53.1 ± 10.4 years and 268 women aged 54.0 ± 10.9 years. A structured questionnaire on demographic, socioeconomic, psychosocial factors, and health behaviors was administered. Physical measurements included anthropometrics and blood pressure. Venous blood and adipose tissue aspirations from the gluteal region were frozen-stored for future analysis. Multivariable-adjusted logistic regression was used to assess the independent associations of conventional risk factors with acute coronary syndrome. Results Upon adjustment for covariates, family history of coronary heart disease was found to be a strong predictor of acute coronary syndrome in both men (odds ratio [OR], 3.70; 95% confidence interval [CI], 2.58-5.30) and women (OR, 4.53; 2.40-8.57). Waist-to-hip ratio in men (OR, 4.03; 2.83-5.73) and obesity in women (OR, 3.31; 1.54-7.14) were strongly associated with acute coronary syndrome. In men, but not in women, there was a significant association with hypertension and current smoking (P = 0.011 and P<0.001, respectively). Diabetes was not significantly independently associated in either sex. Conclusion Classical risk factors predicted coronary heart disease in Albania, similarly as in the rest of the world, although associations with family history and anthropometric indices were stronger. These findings are resulting largely from the heterogeneous

  3. Comparative effectiveness of a complex Ayurvedic treatment and conventional standard care in osteoarthritis of the knee – study protocol for a randomized controlled trial

    PubMed Central

    2013-01-01

    Background Traditional Indian Ayurvedic medicine uses complex treatment approaches, including manual therapies, lifestyle and nutritional advice, dietary supplements, medication, yoga, and purification techniques. Ayurvedic strategies are often used to treat osteoarthritis (OA) of the knee; however, no systematic data are available on their effectiveness in comparison with standard care. The aim of this study is to evaluate the effectiveness of complex Ayurvedic treatment in comparison with conventional methods of treating OA symptoms in patients with knee osteoarthritis. Methods and design In a prospective, multicenter, randomized controlled trial, 150 patients between 40 and 70 years, diagnosed with osteoarthritis of the knee, following American College of Rheumatology criteria and an average pain intensity of ≥40 mm on a 100 mm visual analog scale in the affected knee at baseline will be randomized into two groups. In the Ayurveda group, treatment will include tailored combinations of manual treatments, massages, dietary and lifestyle advice, consideration of selected foods, nutritional supplements, yoga posture advice, and knee massage. Patients in the conventional group will receive self-care advice, pain medication, weight-loss advice (if overweight), and physiotherapy following current international guidelines. Both groups will receive 15 treatment sessions over 12 weeks. Outcomes will be evaluated after 6 and 12 weeks and 6 and 12 months. The primary endpoint is a change in the score on the Western Ontario and McMaster University Osteoarthritis Index (WOMAC) after 12 weeks. Secondary outcome measurements will use WOMAC subscales, a pain disability index, a visual analog scale for pain and sleep quality, a pain experience scale, a quality-of-life index, a profile of mood states, and Likert scales for patient satisfaction, patient diaries, and safety. Using an adapted PRECIS scale, the trial was identified as lying mainly in the middle of the efficacy

  4. Efficacy and safety of femtosecond laser-assisted cataract surgery versus conventional phacoemulsification for cataract: a meta-analysis of randomized controlled trials.

    PubMed

    Chen, Xiaoyun; Xiao, Wei; Ye, Shaobi; Chen, Weirong; Liu, Yizhi

    2015-01-01

    The aim of this study was to evaluate the efficacy and safety of femtosecond laser-assisted cataract surgery (FLACS) versus conventional phacoemulsification cataract surgery (CPCS) in the treatment of cataract. Randomized controlled trials (RCTs) were searched in PubMed, Embase and the Cochrane Central Register of Controlled Trials. Nine qualified studies with a total of 989 eyes were included. Compared with CPCS, FLACS significantly reduced mean phaco energy and effective phacoemulsification time (EPT) required in the surgery. Central corneal thickness (CCT) was significantly lower in FLACS at 1 day of follow-up, but CCT and corneal endothelial cells count was comparable at 1 week of follow-up or longer. FLACS achieved a better visual outcome at postoperative 1 week and 6 months, but the difference was not significant at postoperative 1-3 months. Regard to surgical complications, the incidences of intraoperative anterior capsule tear, postoperative macular edema and elevated intraocular pressure were similar. In conclusion, femtosecond laser pretreatment can reduce phaco energy and EPT, which may reduce the heat damage to ocular tissues by ultrasound. This novel technique might be beneficial for patients with dense cataract and/or low preoperative endothelial cell values. Well-designed RCTs with longer follow-up are still necessary to provide more reliable evidence. PMID:26269445

  5. Country tobacco laws and article 11 of the WHO Framework Convention on Tobacco Control: a review of tobacco packaging and labeling regulations of 25 countries

    PubMed Central

    2013-01-01

    Background Urgent, evidence-based tobacco control efforts have been advocated by the WHO through the Framework Convention on Tobacco Control (FCTC) articles and guidelines. The level of implementation of these guidelines varies by country and region. This paper identifies areas of alignment and non-alignment of country tobacco laws with respect to the FCTC’s article 11 requirements, which lists guidelines for regulating tobacco packaging and labeling. Methods Countries from each of the six WHO regions were ranked by number of smokers and 25 countries were selected, representing countries from all WHO regions with the highest number of smokers. A scoring guide based on the FCTC article 11 requirements was created and used to rank country tobacco laws and assess levels of alignment as well as identify common areas of weakness and strength. Results Across the countries examined, laws were generally strong in mandating the display of health warning messages on the front and back of cigarette packs and cartons. However, they were deficient in prohibiting the display of emission yields, and placing warnings at the top of the principal display area, as well as requiring health messages on tobacco’s negative social and economic outcomes. Conclusion Country tobacco packaging and labeling laws can be strengthened by greater compliance with the FCTC article 11 guidelines. PMID:24195752

  6. Design of exceptionally strong and conductive Cu alloys beyond the conventional speculation via the interfacial energy-controlled dispersion of γ-Al2O3 nanoparticles

    PubMed Central

    Zeon Han, Seung; Kim, Kwang Ho; Kang, Joonhee; Joh, Hongrae; Kim, Sang Min; Ahn, Jee Hyuk; Lee, Jehyun; Lim, Sung Hwan; Han, Byungchan

    2015-01-01

    The development of Cu-based alloys with high-mechanical properties (strength, ductility) and electrical conductivity plays a key role over a wide range of industrial applications. Successful design of the materials, however, has been rare due to the improvement of mutually exclusive properties as conventionally speculated. In this paper, we demonstrate that these contradictory material properties can be improved simultaneously if the interfacial energies of heterogeneous interfaces are carefully controlled. We uniformly disperse γ-Al2O3 nanoparticles over Cu matrix, and then we controlled atomic level morphology of the interface γ-Al2O3//Cu by adding Ti solutes. It is shown that the Ti dramatically drives the interfacial phase transformation from very irregular to homogeneous spherical morphologies resulting in substantial enhancement of the mechanical property of Cu matrix. Furthermore, the Ti removes impurities (O and Al) in the Cu matrix by forming oxides leading to recovery of the electrical conductivity of pure Cu. We validate experimental results using TEM and EDX combined with first-principles density functional theory (DFT) calculations, which all consistently poise that our materials are suitable for industrial applications. PMID:26616045

  7. Efficacy and safety of femtosecond laser-assisted cataract surgery versus conventional phacoemulsification for cataract: a meta-analysis of randomized controlled trials

    PubMed Central

    Chen, Xiaoyun; Xiao, Wei; Ye, Shaobi; Chen, Weirong; Liu, Yizhi

    2015-01-01

    The aim of this study was to evaluate the efficacy and safety of femtosecond laser-assisted cataract surgery (FLACS) versus conventional phacoemulsification cataract surgery (CPCS) in the treatment of cataract. Randomized controlled trials (RCTs) were searched in PubMed, Embase and the Cochrane Central Register of Controlled Trials. Nine qualified studies with a total of 989 eyes were included. Compared with CPCS, FLACS significantly reduced mean phaco energy and effective phacoemulsification time (EPT) required in the surgery. Central corneal thickness (CCT) was significantly lower in FLACS at 1 day of follow-up, but CCT and corneal endothelial cells count was comparable at 1 week of follow-up or longer. FLACS achieved a better visual outcome at postoperative 1 week and 6 months, but the difference was not significant at postoperative 1–3 months. Regard to surgical complications, the incidences of intraoperative anterior capsule tear, postoperative macular edema and elevated intraocular pressure were similar. In conclusion, femtosecond laser pretreatment can reduce phaco energy and EPT, which may reduce the heat damage to ocular tissues by ultrasound. This novel technique might be beneficial for patients with dense cataract and/or low preoperative endothelial cell values. Well-designed RCTs with longer follow-up are still necessary to provide more reliable evidence. PMID:26269445

  8. Fast track multi-discipline treatment (FTMDT trial) versus conventional treatment in colorectal cancer--the design of a prospective randomized controlled study

    PubMed Central

    2011-01-01

    Background Laparoscopy-assisted surgery, fast-track perioperative treatment are both increasingly used in colorectal cancer treatment, for their short-time benefits of enhanced recovery and short hospital stays. However, the benefits of the integration of the Laparoscopy-assisted surgery, fast-track perioperative treatment, and even with the Xelox chemotherapy, are still unknown. In this study, the three treatments integration is defined as "Fast Track Multi-Discipline Treatment Model" for colorectal cancer and this model extends the benefits to the whole treatment process of colorectal cancer. The main purpose of the study is to explore the feasibility of "Fast Track Multi-Discipline Treatment" model in treatment of colorectal cancer. Methods The trial is a prospective randomized controlled study with 2 × 2 balanced factorial design. Patients eligible for the study will be randomized to 4 groups: (I) Laparoscopic surgery with fast track perioperative treatment and Xelox chemotherapy; (II) Open surgery with fast track perioperative treatment and Xelox chemotherapy; (III) Laparoscopic surgery with conventional perioperative treatment and mFolfox6 chemotherapy; (IV) Open surgery with conventional perioperative treatment and mFolfox6 chemotherapy. The primary endpoint of this study is the hospital stays. The secondary endpoints are the quality of life, chemotherapy related adverse events, surgical complications and hospitalization costs. Totally, 340 patients will be enrolled with 85 patients in each group. Conclusions The study initiates a new treatment model "Fast Track Multi-Discipline Treatment" for colorectal cancer, and will provide feasibility evidence on the new model "Fast Track Multi-Discipline Treatment" for patients with colorectal cancer. Trial registration ClinicalTrials.gov: NCT01080547 PMID:22111914

  9. Predicting survival in heart failure case and control subjects by use of fully automated methods for deriving nonlinear and conventional indices of heart rate dynamics

    NASA Technical Reports Server (NTRS)

    Ho, K. K.; Moody, G. B.; Peng, C. K.; Mietus, J. E.; Larson, M. G.; Levy, D.; Goldberger, A. L.

    1997-01-01

    BACKGROUND: Despite much recent interest in quantification of heart rate variability (HRV), the prognostic value of conventional measures of HRV and of newer indices based on nonlinear dynamics is not universally accepted. METHODS AND RESULTS: We have designed algorithms for analyzing ambulatory ECG recordings and measuring HRV without human intervention, using robust methods for obtaining time-domain measures (mean and SD of heart rate), frequency-domain measures (power in the bands of 0.001 to 0.01 Hz [VLF], 0.01 to 0.15 Hz [LF], and 0.15 to 0.5 Hz [HF] and total spectral power [TP] over all three of these bands), and measures based on nonlinear dynamics (approximate entropy [ApEn], a measure of complexity, and detrended fluctuation analysis [DFA], a measure of long-term correlations). The study population consisted of chronic congestive heart failure (CHF) case patients and sex- and age-matched control subjects in the Framingham Heart Study. After exclusion of technically inadequate studies and those with atrial fibrillation, we used these algorithms to study HRV in 2-hour ambulatory ECG recordings of 69 participants (mean age, 71.7+/-8.1 years). By use of separate Cox proportional-hazards models, the conventional measures SD (P<.01), LF (P<.01), VLF (P<.05), and TP (P<.01) and the nonlinear measure DFA (P<.05) were predictors of survival over a mean follow-up period of 1.9 years; other measures, including ApEn (P>.3), were not. In multivariable models, DFA was of borderline predictive significance (P=.06) after adjustment for the diagnosis of CHF and SD. CONCLUSIONS: These results demonstrate that HRV analysis of ambulatory ECG recordings based on fully automated methods can have prognostic value in a population-based study and that nonlinear HRV indices may contribute prognostic value to complement traditional HRV measures.

  10. Near-infrared fluorescence cholangiography assisted laparoscopic cholecystectomy versus conventional laparoscopic cholecystectomy (FALCON trial): study protocol for a multicentre randomised controlled trial

    PubMed Central

    van den Bos, Jacqueline; Schols, Rutger M; Luyer, Misha D; van Dam, Ronald M; Vahrmeijer, Alexander L; Meijerink, Wilhelmus J; Gobardhan, Paul D; van Dam, Gooitzen M; Bouvy, Nicole D; Stassen, Laurents P S

    2016-01-01

    Introduction Misidentification of the extrahepatic bile duct anatomy during laparoscopic cholecystectomy (LC) is the main cause of bile duct injury. Easier intraoperative recognition of the biliary anatomy may be accomplished by using near-infrared fluorescence (NIRF) imaging after an intravenous injection of indocyanine green (ICG). Promising results were reported for successful intraoperative identification of the extrahepatic bile ducts compared to conventional laparoscopic imaging. However, routine use of ICG fluorescence laparoscopy has not gained wide clinical acceptance yet due to a lack of high-quality clinical data. Therefore, this multicentre randomised clinical study was designed to assess the potential added value of the NIRF imaging technique during LC. Methods and analysis A multicentre, randomised controlled clinical trial will be carried out to assess the use of NIRF imaging in LC. In total, 308 patients scheduled for an elective LC will be included. These patients will be randomised into a NIRF imaging laparoscopic cholecystectomy (NIRF-LC) group and a conventional laparoscopic cholecystectomy (CLC) group. The primary end point is time to ‘critical view of safety’ (CVS). Secondary end points are ‘time to identification of the cystic duct (CD), of the common bile duct, the transition of CD in the gallbladder and the transition of the cystic artery in the gallbladder, these all during dissection of CVS’; ‘total surgical time’; ‘intraoperative bile leakage from the gallbladder or cystic duct’; ‘bile duct injury’; ‘postoperative length of stay’, ‘complications due to the injected ICG’; ‘conversion to open cholecystectomy’; ‘postoperative complications (until 90 days postoperatively)’ and ‘cost-minimisation’. Ethics and dissemination The protocol has been approved by the Medical Ethical Committee of Maastricht University Medical Center/Maastricht University; the trial has been registered at Clinical

  11. Cost-effectiveness of microendoscopic discectomy versus conventional open discectomy in the treatment of lumbar disc herniation: a prospective randomised controlled trial [ISRCTN51857546

    PubMed Central

    Arts, Mark P; Peul, Wilco C; Brand, Ronald; Koes, Bart W; Thomeer, Ralph TWM

    2006-01-01

    Background Open discectomy is the standard surgical procedure in the treatment of patients with long-lasting sciatica caused by lumbar disc herniation. Minimally invasive approaches such as microendoscopic discectomy have gained attention in recent years. Reduced tissue trauma allows early ambulation, short hospital stay and quick resumption of daily activities. A comparative cost-effectiveness study has not been performed yet. We present the design of a randomised controlled trial on cost-effectiveness of microendoscopic discectomy versus conventional open discectomy in patients with lumbar disc herniation. Methods/Design Patients (age 18–70 years) presenting with sciatica due to lumbar disc herniation lasting more than 6–8 weeks are included. Patients with disc herniation larger than 1/3 of the spinal canal diameter, or disc herniation less than 1/3 of the spinal canal diameter with concomitant lateral recess stenosis or sequestration, are eliglible for participation. Randomisation into microendoscopic discectomy or conventional unilateral transflaval discectomy will take place in the operating room after induction of anesthesia. The length of skin incision is equal in both groups. The primary outcome measure is the functional assessment of the patient, measured by the Roland Disability Questionnaire for Sciatica, at 8 weeks and 1 year after surgery. We will also evaluate several other outcome parameters, including perceived recovery, leg and back pain, incidence of re-operations, complications, serum creatine kinase, quality of life, medical consumption, absenteeism and costs. The study is a randomised prospective multi-institutional trial, in which two surgical techniques are compared in a parallel group design. Patients and research nurses are kept blinded of the allocated treatment during the follow-up period of 2 years. Discussion Currently, open discectomy is the golden standard in the surgical treatment of lumbar disc herniation. Whether

  12. A direct current superconducting quantum interference device gradiometer with a digital signal processor controlled flux-locked loop and comparison with a conventional analog feedback scheme

    SciTech Connect

    Kung, P.J.; Bracht, R.R.; Flynn, E.R.; Lewis, P.S.

    1996-01-01

    A double-washer dc superconducting quantum interference device (SQUID) gradiometer with a flux-locked loop (FLL) based on a digital signal processor (DSP) has been developed for biomagnetic applications. All of the analog electronics in the conventional FLL are replaced and implemented by the DSP except for the low-noise field-effect transistor preamplifier at the front end of the signal recovery components. The DSP performs the signal demodulation by synchronously sampling the recovered signals and applying the appropriate full wave rectification. The signals are then integrated, filtered, and applied to the output. At 4.2 K, the white flux noise of the gradiometer measured in a DSP FLL mode is about 4{mu}{phi}{sub 0}/{radical}Hz and the noise at 1 Hz is 13 {mu}{phi}{sub 0}/{radical}Hz. The corresponding noise levels in the gradiometer operated by the conventional FLL are 1.8 and 3{mu}{phi}{sub 0}/{radical}Hz. The poorer system performance in the DSP FLL compared to the analog FLL is mainly caused by the ambient field noise and interference signals picked up through the connecting cables. Additional noise is also added to the overall noise floor by the instruments employed in the DSP system in the present prototype setup. Further improvement in the noise characteristics and the dynamic behavior of the DSP SQUID gradiometer is expected when a better configuration of DSP with the associated I/O devices is implemented. Additional improvements of the DSP programs are expected by incorporating higher-order integration, adaptive control, and noise reduction schemes. {copyright} {ital 1996 American Institute of Physics.}

  13. High-performance, lattice-mismatched InGaAs/InP monolithic interconnected modules (MIMs)

    SciTech Connect

    Fatemi, Navid S.; Wilt, David M.; Hoffman, Richard W., Jr.; Stan, Mark S.; Weizer, Victor G.; Jenkins, Phillip P.; Khan, Osman S.; Murray, Christopher S.; Scheiman, David; Brinker, David

    1998-10-01

    High performance, lattice-mismatched p/n InGaAs/lnP monolithic interconnected module (MIM) structures were developed for thermophotovoltaic (TPV) applications. A MIM device consists of several individual InGaAs photovoltaic (PV) cells series-connected on a single semi-insulating (S.I.) InP substrate. Both interdigitated and conventional (i.e., non-interdigitated) MIMs were fabricated. The energy bandgap (Eg) for these devices was 0.60 eV. A compositionally step-graded InPAs buffer was used to accommodate a lattice mismatch of 1.1% between the active InGaAs cell structure and the InP substrate. 1x1-cm, 15-cell, 0.60-eV MIMs demonstrated an open-circuit voltage (Voc) of 5.2 V (347 mV per cell) and a fill factor of 68.6% at a short-circuit current density (Jsc) of 2.0 A/cm{sup 2}, under flashlamp testing. The reverse saturation current density (Jo) was 1.6x10{sup {minus}6} A/cm{sup 2}. Jo values as low as 4.1x10{sup {minus}7} A/cm{sup 2} were also observed with a conventional planar cell geometry.

  14. High-performance, lattice-mismatched InGaAs/InP monolithic interconnected modules (MIMs)

    SciTech Connect

    Fatemi, Navid S.; Wilt, David M.; Hoffman, Richard W.; Stan, Mark A.; Weizer, Victor G.; Jenkins, Phillip P.; Khan, Osman S.; Brinker, David Murray, Christopher S.

    1999-03-01

    High performance, lattice-mismatched p/n InGaAs/InP monolithic interconnected module (MIM) structures were developed for thermophotovoltaic (TPV) applications. A MIM device consists of several individual InGaAs photovoltaic (PV) cells series-connected on a single semi-insulating (S.I.) InP substrate. Both interdigitated and conventional (i.e., non-interdigitated) MIMs were fabricated. The energy bandgap (Eg) for these devices was 0.60 eV. A compositionally step-graded InPAs buffer was used to accommodate a lattice mismatch of 1.1{percent} between the active InGaAs cell structure and the InP substrate. 1{times}1-cm, 15-cell, 0.60-eV MIMs demonstrated an open-circuit voltage (Voc) of 5.2 V (347 mV per cell) and a fill factor of 68.6{percent} at a short-circuit current density (Jsc) of 2.0 A/cm{sup 2}, under flashlamp testing. The reverse saturation current density (Jo) was 1.6{times}10{sup {minus}6}&hthinsp;A/cm{sup 2}. Jo values as low as 4.1{times}10{sup {minus}7}&hthinsp;A/cm{sup 2} were also observed with a conventional planar cell geometry. {copyright} {ital 1999 American Institute of Physics.}

  15. Compensating Acoustic Mismatch Using Class-Based Histogram Equalization for Robust Speech Recognition

    NASA Astrophysics Data System (ADS)

    Suh, Youngjoo; Kim, Sungtak; Kim, Hoirin

    2007-12-01

    A new class-based histogram equalization method is proposed for robust speech recognition. The proposed method aims at not only compensating for an acoustic mismatch between training and test environments but also reducing the two fundamental limitations of the conventional histogram equalization method, the discrepancy between the phonetic distributions of training and test speech data, and the nonmonotonic transformation caused by the acoustic mismatch. The algorithm employs multiple class-specific reference and test cumulative distribution functions, classifies noisy test features into their corresponding classes, and equalizes the features by using their corresponding class reference and test distributions. The minimum mean-square error log-spectral amplitude (MMSE-LSA)-based speech enhancement is added just prior to the baseline feature extraction to reduce the corruption by additive noise. The experiments on the Aurora2 database proved the effectiveness of the proposed method by reducing relative errors by[InlineEquation not available: see fulltext.] over the mel-cepstral-based features and by[InlineEquation not available: see fulltext.] over the conventional histogram equalization method, respectively.

  16. The visual mismatch negativity elicited with visual speech stimuli

    PubMed Central

    Files, Benjamin T.; Auer, Edward T.; Bernstein, Lynne E.

    2013-01-01

    The visual mismatch negativity (vMMN), deriving from the brain's response to stimulus deviance, is thought to be generated by the cortex that represents the stimulus. The vMMN response to visual speech stimuli was used in a study of the lateralization of visual speech processing. Previous research suggested that the right posterior temporal cortex has specialization for processing simple non-speech face gestures, and the left posterior temporal cortex has specialization for processing visual speech gestures. Here, visual speech consonant-vowel (CV) stimuli with controlled perceptual dissimilarities were presented in an electroencephalography (EEG) vMMN paradigm. The vMMNs were obtained using the comparison of event-related potentials (ERPs) for separate CVs in their roles as deviant vs. their roles as standard. Four separate vMMN contrasts were tested, two with the perceptually far deviants (i.e., “zha” or “fa”) and two with the near deviants (i.e., “zha” or “ta”). Only far deviants evoked the vMMN response over the left posterior temporal cortex. All four deviants evoked vMMNs over the right posterior temporal cortex. The results are interpreted as evidence that the left posterior temporal cortex represents speech contrasts that are perceived as different consonants, and the right posterior temporal cortex represents face gestures that may not be perceived as different CVs. PMID:23882205

  17. Mismatch Negativity (MMN) as an Index of Cognitive Dysfunction

    PubMed Central

    Näätänen, Risto; Sussman, Elyse S.; Salisbury, Dean; Shafer, Valerie L.

    2014-01-01

    Cognition is often affected in a variety of neuropsychiatric, neurological, and neurodevelopmental disorders. The neural discriminative response, reflected in mismatch negativity (MMN) and its magnetoencephalographic equivalent (MMNm), has been used as a tool to study a variety of disorders involving auditory cognition. MMN/MMNm is an involuntary brain response to auditory change or, more generally, to pattern regularity violation. For a number of disorders, MMN/MMNm amplitude to sound deviance has been shown to be attenuated or the peak-latency of the component prolonged compared to controls. This general finding suggests that while not serving as a specific marker to any particular disorder, MMN may be useful for understanding factors of cognition in various disorders, and has potential to serve as an indicator of risk. This review presents a brief history of the MMN, followed by a description of how MMN has been used to index auditory processing capability in a range of neuropsychiatric, neurological, and neurodevelopmental disorders. Finally, we suggest future directions for research to further enhance our understanding of the neural substrate of deviance detection that could lead to improvements in the use of MMN as a clinical tool. PMID:24838819

  18. Anterior insula coordinates hierarchical processing of tactile mismatch responses

    PubMed Central

    Allen, Micah; Fardo, Francesca; Dietz, Martin J.; Hillebrandt, Hauke; Friston, Karl J.; Rees, Geraint; Roepstorff, Andreas

    2016-01-01

    The body underlies our sense of self, emotion, and agency. Signals arising from the skin convey warmth, social touch, and the physical characteristics of external stimuli. Surprising or unexpected tactile sensations can herald events of motivational salience, including imminent threats (e.g., an insect bite) and hedonic rewards (e.g., a caressing touch). Awareness of such events is thought to depend upon the hierarchical integration of body-related mismatch responses by the anterior insula. To investigate this possibility, we measured brain activity using functional magnetic resonance imaging, while healthy participants performed a roving tactile oddball task. Mass-univariate analysis demonstrated robust activations in limbic, somatosensory, and prefrontal cortical areas previously implicated in tactile deviancy, body awareness, and cognitive control. Dynamic Causal Modelling revealed that unexpected stimuli increased the strength of forward connections along a caudal to rostral hierarchy—projecting from thalamic and somatosensory regions towards insula, cingulate and prefrontal cortices. Within this ascending flow of sensory information, the AIC was the only region to show increased backwards connectivity to the somatosensory cortex, augmenting a reciprocal exchange of neuronal signals. Further, participants who rated stimulus changes as easier to detect showed stronger modulation of descending PFC to AIC connections by deviance. These results suggest that the AIC coordinates hierarchical processing of tactile prediction error. They are interpreted in support of an embodied predictive coding model where AIC mediated body awareness is involved in anchoring a global neuronal workspace. PMID:26584870

  19. Anterior insula coordinates hierarchical processing of tactile mismatch responses.

    PubMed

    Allen, Micah; Fardo, Francesca; Dietz, Martin J; Hillebrandt, Hauke; Friston, Karl J; Rees, Geraint; Roepstorff, Andreas

    2016-02-15

    The body underlies our sense of self, emotion, and agency. Signals arising from the skin convey warmth, social touch, and the physical characteristics of external stimuli. Surprising or unexpected tactile sensations can herald events of motivational salience, including imminent threats (e.g., an insect bite) and hedonic rewards (e.g., a caressing touch). Awareness of such events is thought to depend upon the hierarchical integration of body-related mismatch responses by the anterior insula. To investigate this possibility, we measured brain activity using functional magnetic resonance imaging, while healthy participants performed a roving tactile oddball task. Mass-univariate analysis demonstrated robust activations in limbic, somatosensory, and prefrontal cortical areas previously implicated in tactile deviancy, body awareness, and cognitive control. Dynamic Causal Modelling revealed that unexpected stimuli increased the strength of forward connections along a caudal to rostral hierarchy-projecting from thalamic and somatosensory regions towards insula, cingulate and prefrontal cortices. Within this ascending flow of sensory information, the AIC was the only region to show increased backwards connectivity to the somatosensory cortex, augmenting a reciprocal exchange of neuronal signals. Further, participants who rated stimulus changes as easier to detect showed stronger modulation of descending PFC to AIC connections by deviance. These results suggest that the AIC coordinates hierarchical processing of tactile prediction error. They are interpreted in support of an embodied predictive coding model where AIC mediated body awareness is involved in anchoring a global neuronal workspace. PMID:26584870

  20. Meniscus replacement: Influence of geometrical mismatches on chondroprotective capabilities.

    PubMed

    Párraga Quiroga, J M; Ito, K; van Donkelaar, C C

    2015-06-01

    The chondroprotective success of meniscal transplantation is variable. Poorly controlled factors such as a geometrical mismatch of the implant may be partly responsible. Clinical data, animal studies and cadaver experiments suggest that smaller transplants perform better than oversized, but clear evidence is lacking. The hypothesis of this study is that smaller menisci outperform larger ones because they distribute stresses more effectively at those particular locations that receive the highest loads. Consequently, collagen in the adjacent cartilage is protected from damage due to overstraining. Experimentally it is not possible to measure load distribution and collagen strain inside articular cartilage (AC). Therefore, a numerical model was used to determine the mechanical conditions throughout the depth of the AC. Meniscus implants with different sizes and mechanical properties were evaluated. These were compared with healthy and with meniscectomized joints. To account for the time-dependent behavior 600s of loading was simulated; results were visualized after 1s and 600s. Simulations showed that AC's strains strongly depended on implant size and loading duration. They depended less on the stiffness of the implant material. With an oversized implant, collagen strains were particularly large in the femoral AC initially and further increased upon sustained loading. The severest compressive strains occurred after sustained loading in the meniscectomized joint. Strains with an undersized meniscus were comparable to a perfectly sized implant. In conclusion, these results support the hypothesis that an undersized implant may outperform an oversized one because it distributes stresses better in the most intensely loaded joint area. PMID:25835788

  1. Sympathetic cooling of a mass-mismatched two-ion chain in a double-well trap potential

    SciTech Connect

    Hasegawa, Taro

    2011-05-15

    Sympathetic cooling of two-ion system, in which one is laser-cooled and the other is sympathetically cooled and their masses are mismatched, in a linear rf trap with a double-well potential is proposed. The double-well potential consists of two wells, and there is one ion in each well. The axial frequencies of the two wells and the spatial interval between them are experimentally controllable. By theoretical analysis, the normal modes of the small oscillations around the equilibrium are derived, and a measure of the sympathetic cooling rate is obtained. As a result, it is found that the sympathetic cooling rate is fast when the frequency of the axial motion of the sympathetically cooled ion is close to that of the laser-cooled ion. In the double-well potential, the sympathetic cooling rate of the ion species whose mass is much heavier or lighter than that of the laser-cooled ion can be fast. The sympathetic cooling rate of C{sub 60}{sup +} by the laser-cooled Ba{sup +} in the double-well potential is estimated to be about 80 times faster than in the conventional setup. The double-well potential may be made by the microfabricated electrode configuration or by the optical dipole force trap.

  2. Mismatched dNTP incorporation by DNA polymerase [beta] does not proceed via globally different conformational pathways

    SciTech Connect

    Tang, Kuo-Hsiang; Niebuhr, Marc; Tung, Chang-Shung; Chan, Hsiu-chien; Chou, Chia-Cheng; Tsai, Ming-Daw

    2008-07-07

    Understanding how DNA polymerases control fidelity requires elucidation of the mechanisms of matched and mismatched dNTP incorporations. Little is known about the latter because mismatched complexes do not crystallize readily. In this report, we employed small-angle X-ray scattering (SAXS) and structural modeling to probe the conformations of different intermediate states of mammalian DNA polymerase {beta} (Pol {beta}) in its wild-type and an error-prone variant, I260Q. Our structural results indicate that the mismatched ternary complex lies in-between the open and the closed forms, but more closely resembles the open form for WT and the closed form for I260Q. On the basis of molecular modeling, this over-stabilization of mismatched ternary complex of I260Q is likely caused by formation of a hydrogen bonding network between the side chains of Gln{sup 260}, Tyr{sup 296}, Glu{sup 295} and Arg{sup 258}, freeing up Asp{sup 192} to coordinate MgdNTP. These results argue against recent reports suggesting that mismatched dNTP incorporations follow a conformational path distinctly different from that of matched dNTP incorporation, or that its conformational closing is a major contributor to fidelity.

  3. SEATCA Tobacco Industry Interference Index: a tool for measuring implementation of WHO Framework Convention on Tobacco Control Article 5.3

    PubMed Central

    Assunta, Mary; Dorotheo, E Ulysses

    2016-01-01

    Objective To measure the implementation of WHO Framework Convention on Tobacco Control (FCTC) Article 5.3 at country level using a new Tobacco Industry Interference Index and to report initial results using this index in seven Southeast Asian countries. Methods Score sheet based on WHO FCTC Article 5.3 Guidelines sent to correspondents in seven Southeast Asian countries, using a scoring system designed with the help of tobacco control experts and validated through focused group discussions. Results The seven countries ranked from the lowest level of interference to the highest are Brunei, Thailand, Lao PDR, Cambodia, Philippines, Malaysia and Indonesia. Countries that face high levels of unnecessary interaction with the tobacco industry also face high levels of tobacco industry influence in policy development. Most governments do not allow any tobacco industry representatives on their delegation to sessions of the Conference of the Parties or its subsidiary bodies nor accept their sponsorship for delegates, but most governments still accept or endorse offers of assistance from the tobacco industry in implementing tobacco control policies. Most governments also receive tobacco industry contributions (monetary or in kind) or endorse industry corporate social responsibility activities. Governments do not have a procedure for disclosing interactions with the tobacco industry, but Lao PDR, Philippines and Thailand have instituted measures to prevent or reduce industry interference. Conclusions This Tobacco Industry Interference Index, based on the WHO FCTC Article 5.3 Guidelines, is a useful advocacy tool for identifying both progress and gaps in national efforts at implementing WHO FCTC Article 5.3. PMID:25908597

  4. Effectiveness of Peer Group and Conventional Method (Dentist) of Oral Health Education Programme Among 12-15 year Old School Children - A Randomized Controlled Trial

    PubMed Central

    Jha, Abhishek; Raju, Rekha; Bashyam, Mamtha

    2016-01-01

    Introduction Oral Health Education (OHE) in schools is routinely delivered by the dentist. Another approach which can be cost-effective, easily accessible and equally effective is the trained group of peer students. Aim The objective of the present study was to assess and compare the effectiveness of peer–led and conventional method (dentist-led), OHE on oral health status, oral health knowledge, attitude and practices among 12-15 year old government school children in Bengaluru South Zone-I at baseline, 3 months and 6 months. Materials and Methods The study population comprised of 450 subjects, 150 each in peer, dentist and control group. At baseline, a pre-tested 14 item questionnaire was used to assess the existing oral health knowledge, attitude and oral hygiene practices of the subjects. Clinical examination included recording of plaque index and gingival index, by a pre-calibrated examiner. OHE was provided by the peer group and dentist (using power-point presentation, chalk and talk presentation, using charts, posters, booklets and tooth brushing demonstration models). Data was analyzed using Kruskal Wallis and Chi-square test. Results Both the peer-led and dentist-led OHE intervention were effective in improving oral health knowledge, attitude, oral hygiene practices and oral health status at three and six months when compared to control group. The adolescents in the peer-led group, however, exhibited statistically better oral health behavior than their counterparts in the dentist-led group and control group. Conclusion The two educator-led strategies (peer group and dentist) had a modest effect on the outcome variables included in the study, the results provide some evidence to show that the peer-led strategy may provide a feasible and almost equally effective alternative to the traditional dentist led strategy of oral health education. PMID:27437345

  5. Mismatch-mediated error prone repair at the Immunoglobulin genes

    PubMed Central

    Chahwan, Richard; Edelmann, Winfried; Scharff, Matthew D; Roa, Sergio

    2011-01-01

    The generation of effective antibodies depends upon somatic hypermutation (SHM) and class-switch recombination (CSR) of antibody genes by activation induced cytidine deaminase (AID) and the subsequent recruitment of error prone base excision and mismatch repair. While AID initiates and is required for SHM, more than half of the base changes that accumulate in V regions are not due to the direct deamination of dC to dU by AID, but rather arise through the recruitment of the mismatch repair complex (MMR) to the U:G mismatch created by AID and the subsequent perversion of mismatch repair from a high fidelity process to one that is very error prone. In addition, the generation of double-strand breaks (DSBs) is essential during CSR, and the resolution of AID-generated mismatches by MMR to promote such DSBs is critical for the efficiency of the process. While a great deal has been learned about how AID and MMR cause hypermutations and DSBs, it is still unclear how the error prone aspect of these processes is largely restricted to antibody genes. The use of knockout models and mice expressing mismatch repair proteins with separation-of-function point mutations have been decisive in gaining a better understanding of the roles of each of the major MMR proteins and providing further insight into how mutation and repair are coordinated. Here, we review the cascade of MMR factors and repair signals that are diverted from their canonical error free role and hijacked by B cells to promote genetic diversification of the Ig locus. This error prone process involves AID as the inducer of enzymatically-mediated DNA mismatches, and a plethora of downstream MMR factors acting as sensors, adaptors and effectors of a complex and tightly regulated process from much of which is not yet well understood. PMID:22100214

  6. HLA Haplotype Mismatch Transplants and Posttransplant Cyclophosphamide

    PubMed Central

    Bacigalupo, Andrea; Sica, Simona

    2016-01-01

    The use of high dose posttransplant cyclophosphamide (PT-CY) introduced by the Baltimore group approximately 10 years ago has been rapidly adopted worldwide and is becoming a standard for patients undergoing unmanipulated haploidentical (HAPLO) transplants. PT-CY has been used following nonmyeloablative as well as myeloablative conditioning regimens, for bone marrow or peripheral blood grafts, for patients with malignant and nonmalignant disorders. Retrospective comparisons of HAPLO grafts with conventional sibling and unrelated donor grafts have been published and suggest comparable outcome. The current questions to be answered include the use of PT-CY for sibling and unrelated donors transplant, possibly in the context of prospective randomized trial. PMID:27143973

  7. Instrument-free control of the standard potential of potentiometric solid-contact ion-selective electrodes by short-circuiting with a conventional reference electrode.

    PubMed

    Vanamo, Ulriika; Bobacka, Johan

    2014-11-01

    A simple, instrument-free method to control the standard potential (E°) of potentiometric solid-contact ion-selective electrodes (SC-ISE) is described. In this method, the electrode potential of a SC-ISE is reset by short-circuiting the electrode with a metallic wire to a conventional Ag/AgCl/3 M KCl reference electrode (RE) in a solution containing primary ions. The method is studied experimentally for SC-ISEs where the conducting polymer poly(3,4-ethylenedioxythiophene) doped with the bulky anion poly(sodium 4-styrenesulfonate), PEDOT(PSS), is used as the solid contact. Three different types of ion-selective membranes (ISMs) are studied: two potassium-selective membranes, with and without the lipohilic additive tetradodecylammonium tetrakis(4-chlorophenyl)borate (ETH-500) and a cation-sensitive membrane without an ionophore. When the SC-ISE is short-circuited with the RE, the PEDOT(PSS) layer is oxidized or reduced, thereby shifting the potential of the SC-ISE to the proximity of the potential of the RE so that the potential difference between these two electrodes becomes zero or close to zero. The slope of the calibration curve is preserved after the short-circuit treatment of the SC-ISEs. The short-circuiting method is an important step toward calibration-free potentiometric analysis. PMID:25284311

  8. Enhanced entrainability of genetic oscillators by period mismatch

    PubMed Central

    Hasegawa, Yoshihiko; Arita, Masanori

    2013-01-01

    Biological oscillators coordinate individual cellular components so that they function coherently and collectively. They are typically composed of multiple feedback loops, and period mismatch is unavoidable in biological implementations. We investigated the advantageous effect of this period mismatch in terms of a synchronization response to external stimuli. Specifically, we considered two fundamental models of genetic circuits: smooth and relaxation oscillators. Using phase reduction and Floquet multipliers, we numerically analysed their entrainability under different coupling strengths and period ratios. We found that a period mismatch induces better entrainment in both types of oscillator; the enhancement occurs in the vicinity of the bifurcation on their limit cycles. In the smooth oscillator, the optimal period ratio for the enhancement coincides with the experimentally observed ratio, which suggests biological exploitation of the period mismatch. Although the origin of multiple feedback loops is often explained as a passive mechanism to ensure robustness against perturbation, we study the active benefits of the period mismatch, which include increasing the efficiency of the genetic oscillators. Our findings show a qualitatively different perspective for both the inherent advantages of multiple loops and their essentiality. PMID:23389900

  9. The Effect of Codon Mismatch on the Protein Translation System

    PubMed Central

    Cao, Liaoran; Li, Guohui; Cheng, Hong

    2016-01-01

    Incorrect protein translation, caused by codon mismatch, is an important problem of living cells. In this work, a computational model was introduced to quantify the effects of codon mismatch and the model was used to study the protein translation of Saccharomyces cerevisiae. According to simulation results, the probability of codon mismatch will increase when the supply of amino acids is unbalanced, and the longer is the codon sequence, the larger is the probability for incorrect translation to occur, making the synthesis of long peptide chain difficult. By comparing to simulation results without codon mismatch effects taken into account, the fraction of mRNAs with bound ribosome decrease faster along the mRNAs, making the 5’ ramp phenomenon more obvious. It was also found in our work that the premature mechanism resulted from codon mismatch can reduce the proportion of incorrect translation when the amino acid supply is extremely unbalanced, which is one possible source of high fidelity protein synthesis after peptidyl transfer. PMID:26840415

  10. Differential partial activation phenotype and production of tumour necrosis factor-α by conventional dendritic cells in response to lipopolysaccharide in HIV+ viraemic subjects and HIV+ controllers

    PubMed Central

    Camacho-Sandoval, R; Del Río Estrada, P M; Rivero-Arrieta, A; Reyes-Terán, G; Bonifaz, L C

    2014-01-01

    HIV+ subjects are reported to have increased soluble CD14 (sCD14) in plasma, an indicator of microbial translocation. We evaluated if microbial translocation has a differential impact on the activation and function of conventional dendritic cells (cDC) from viraemic HIV+ subjects and HIV+ controllers (CTs). The HIV+ subjects were classified into two groups according to their plasma viral load (pVL): CT and viraemic. Subjects without HIV were included as controls (HIV–). The frequencies and phenotypes of cDC from these subjects were evaluated by multi-parameter flow cytometry. In addition, peripheral blood mononuclear cells (PBMCs) were stimulated with lipopolysaccharide (LPS) or single-stranded RNA40 (ssRNA40), the phenotype of the cDC and the intracellular production of tumour necrosis factor (TNF)-α by the cDC were evaluated by flow cytometry. We observed a partial activation phenotype for the cDC in the viraemic subjects and CTs ex vivo and after LPS activation, which showed differences in the expression of CD40 and CD86. Furthermore, in response to LPS the cDC from the viraemic subjects produced more TNF-α compared to the cDC from CTs. Interestingly, the percentage of TNF-α+ cDC was found to be correlated positively with the pVL. The partial activation of cDC and the over-production of TNF-α in response to LPS in viraemic HIV+ subjects might be related to the increased chronic activation observed in these subjects. In contrast, cDC from CTs seem to have a regulated response to LPS, indicating that they respond differently to chronic immune activation. These results may have implications in the development of HIV therapies and vaccines using DC. PMID:25130456

  11. Tobacco Packaging and Mass Media Campaigns: Research Needs for Articles 11 and 12 of the WHO Framework Convention on Tobacco Control

    PubMed Central

    2013-01-01

    Introduction: Communicating the health risks of smoking remains a primary objective of tobacco-control policy. Articles 11 and 12 of the World Health Organization’s Framework Convention on Tobacco Control establish standards for two important forms of communication: packaging regulations (Article 11), and mass media campaigns (Article 12). Methods: A narrative review approach was used to identify existing evidence in the areas of package labeling regulations (including health warnings, constituent and emission messages, and prohibitions on misleading information) and communication activities (including mass media campaigns and news media coverage). When available, recent reviews of the literature were used, updated with more recent high-quality studies from published literature. Results: Implementation of Articles 11 and 12 share several important research priorities: (a) identify existing consumer information needs and gaps, (b) research on the message source to identify effective types of content for health warnings and media campaigns, (c) research on how messages are processed and the extent to which the content and form of messages need to be tailored to different cultural and geographic groups, as well as subgroups within countries, and (d) research to identify the most cost-effective mix and best practices for sustaining health communications over time. Conclusion: A unifying theme of effective health communication through tobacco packaging and mass media campaigns is the need to provide salient, timely, and engaging reminders of the consequences of tobacco use in ways that motivate and support tobacco users trying to quit and make tobacco use less appealing for those at risk of taking it up. PMID:23042986

  12. Mismatch Negativity Latency and Cognitive Function in Schizophrenia

    PubMed Central

    Kärgel, Christian; Sartory, Gudrun; Kariofillis, Daniela; Wiltfang, Jens; Müller, Bernhard W.

    2014-01-01

    Background The Mismatch Negativity (MMN) is an event-related potential (ERP) sensitive to early auditory deviance detection and has been shown to be reduced in schizophrenia patients. Moreover, MMN amplitude reduction to duration deviant tones was found to be related to functional outcomes particularly, to neuropsychological (working memory and verbal domains) and psychosocial measures. While MMN amplitude is thought to be correlated with deficits of early sensory processing, the functional significance of MMN latency remains unclear so far. The present study focused on the investigation of MMN in relation to neuropsychological function in schizophrenia. Method Forty schizophrenia patients and 16 healthy controls underwent a passive oddball paradigm (2400 binaural tones; 88% standards [1 kHz, 80 db, 80 ms], 11% frequency deviants [1.2 kHz], 11% duration deviants [40 ms]) and a neuropsychological test-battery. Patients were assessed with regard to clinical symptoms. Results Compared to healthy controls schizophrenia patients showed diminished MMN amplitude and shorter MMN latency to both deviants as well as an impaired neuropsychological test performance. Severity of positive symptoms was related to decreased MMN amplitude to duration deviants. Furthermore, enhanced verbal memory performance was associated with prolonged MMN latency to frequency deviants in patients. Conclusion The present study corroborates previous results of a diminished MMN amplitude and its association with positive symptoms in schizophrenia patients. Both, the findings of a shorter latency to duration and frequency deviants and the relationship of the latter with verbal memory in patients, emphasize the relevance of the temporal aspect of early auditory discrimination processing in schizophrenia. PMID:24740391

  13. Palatal positioned implants in severely atrophic maxillae versus conventional implants to support fixed full-arch prostheses: Controlled retrospective study with 5 years of follow-up

    PubMed Central

    Candel-Marti, Eugenia; Peñarrocha-Oltra, David; Bagán, Leticia; Peñarrocha-Diago, Maria

    2015-01-01

    Background To evaluate soft tissue conditions and bone loss around palatal positioned implants supporting fixed full-arch prostheses to rehabilitate edentulous maxillae with horizontal atrophy and compare them with conventional well-centered implants placed in non-atrophic maxillae after a minimum follow-up of 5 years. Material and Methods A clinical retrospective study was performed of patients that were rehabilitated with full-arch fixed implant-supported maxillary prostheses and had a minimum follow-up of 5 years after implant loading. Patients were divided into 2 groups: patients with class IV maxilla according to Cawood and Howell and treated with palatal positioned implants (test) and with class III maxilla and treated with implants well-centered in the alveolar ridge and completely surrounded by bone (control). The following variables were assessed: age, sex, frequency of tooth brushing, smoking, type of prosthesis, type of implant, implant success, amount of buccal keratinized mucosa, buccal retraction, probing depth, plaque index, modified bleeding index, presence of mucositis or peri-implantitis and peri-implant bone loss. Statistical analysis was performed applying Chi2 Test and Student’s t-test using alpha set at 0.05. Results A total of 57 patients were included: 32 patients with 161 palatal positioned implants (test) and 25 patients with 132 well centered implants (control). No statistically significant differences were found regarding age, sex and smoking, but test group patients reported a significantly higher frequency of daily tooth brushing. Implant success rates were 96.9% for test group implants and 96.0% for control group implants. Peri-implant mucosa retraction was significantly higher in the control group than in the test group (p=0,017). No significant differences were observed either for all the other assessed clinical parameters or for peri-implant bone loss. Conclusions Despite its limitations the outcomes of the present study suggest

  14. Game-Based E-Learning Is More Effective than a Conventional Instructional Method: A Randomized Controlled Trial with Third-Year Medical Students

    PubMed Central

    Boeker, Martin; Andel, Peter; Vach, Werner; Frankenschmidt, Alexander

    2013-01-01

    Background When compared with more traditional instructional methods, Game-based e-learning (GbEl) promises a higher motivation of learners by presenting contents in an interactive, rule-based and competitive way. Most recent systematic reviews and meta-analysis of studies on Game-based learning and GbEl in the medical professions have shown limited effects of these instructional methods. Objectives To compare the effectiveness on the learning outcome of a Game-based e-learning (GbEl) instruction with a conventional script-based instruction in the teaching of phase contrast microscopy urinalysis under routine training conditions of undergraduate medical students. Methods A randomized controlled trial was conducted with 145 medical students in their third year of training in the Department of Urology at the University Medical Center Freiburg, Germany. 82 subjects where allocated for training with an educational adventure-game (GbEl group) and 69 subjects for conventional training with a written script-based approach (script group). Learning outcome was measured with a 34 item single choice test. Students' attitudes were collected by a questionnaire regarding fun with the training, motivation to continue the training and self-assessment of acquired knowledge. Results The students in the GbEl group achieved significantly better results in the cognitive knowledge test than the students in the script group: the mean score was 28.6 for the GbEl group and 26.0 for the script group of a total of 34.0 points with a Cohen's d effect size of 0.71 (ITT analysis). Attitudes towards the recent learning experience were significantly more positive with GbEl. Students reported to have more fun while learning with the game when compared to the script-based approach. Conclusions Game-based e-learning is more effective than a script-based approach for the training of urinalysis in regard to cognitive learning outcome and has a high positive motivational impact on learning. Game

  15. Effects of Blocking D2/D3 Receptors on Mismatch Negativity and P3a Amplitude of Initially Antipsychotic Naïve, First Episode Schizophrenia Patients

    PubMed Central

    Glenthøj, Birte Yding; Oranje, Bob

    2016-01-01

    Background: Reduced mismatch negativity and P3a amplitude have been suggested to be among the core deficits in schizophrenia since the late 1970s. Blockade of dopamine D2 receptors play an important role in the treatment of schizophrenia. In addition, there is some evidence indicating that deficits in mismatch negativity and P3a amplitude are related to increased dopaminergic activity. This is the first study investigating the effect of amisulpride, a potent D2-antagonist, on mismatch negativity and P3a amplitude in a large group of antipsychotic-naïve, first-episode schizophrenia patients. Methods: Fifty-one antipsychotic-naïve, first-episode schizophrenia patients were tested in a mismatch negativity paradigm at baseline and after 6 weeks of treatment with amisulpride. We further examined 48 age- and gender-matched controls in this paradigm. Results: At baseline, the patients showed significantly reduced P3a amplitude compared with healthy controls, but no differences in mismatch negativity. Although the treatment with amisulpride significantly improved the patients’ psychopathological (PANSS) and functional (GAF) scores, it did not influence their mismatch negativity amplitude, while also their reduced P3a amplitude persisted. Conclusion: Our findings show that antipsychotic naïve, first-episode patients with schizophrenia have normal mismatch negativity yet reduced P3a amplitude compared with healthy controls. In spite of the fact that the 6-week amisulpride treatment improved the patients both clinically and functionally, it had no effect on either mismatch negativity or P3a amplitude. This suggests that even though there is a dopaminergic involvement in global functioning and symptomatology in schizophrenia, there is no such involvement in these particular measures of early information processing. PMID:26453696

  16. Forecasting photovoltaic array power production subject to mismatch losses

    SciTech Connect

    Picault, D.; Raison, B.; Bacha, S.; de la Casa, J.; Aguilera, J.

    2010-07-15

    The development of photovoltaic (PV) energy throughout the world this last decade has brought to light the presence of module mismatch losses in most PV applications. Such power losses, mainly occasioned by partial shading of arrays and differences in PV modules, can be reduced by changing module interconnections of a solar array. This paper presents a novel method to forecast existing PV array production in diverse environmental conditions. In this approach, field measurement data is used to identify module parameters once and for all. The proposed method simulates PV arrays with adaptable module interconnection schemes in order to reduce mismatch losses. The model has been validated by experimental results taken on a 2.2 kW{sub p} plant, with three different interconnection schemes, which show reliable power production forecast precision in both partially shaded and normal operating conditions. Field measurements show interest in using alternative plant configurations in PV systems for decreasing module mismatch losses. (author)

  17. Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains

    PubMed Central

    Milovanovic, Dragomir; Honigmann, Alf; Koike, Seiichi; Göttfert, Fabian; Pähler, Gesa; Junius, Meike; Müllar, Stefan; Diederichsen, Ulf; Janshoff, Andreas; Grubmüller, Helmut; Risselada, Herre J.; Eggeling, Christian; Hell, Stefan W.; van den Bogaart, Geert; Jahn, Reinhard

    2015-01-01

    The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic and ionic interactions with membrane lipids as well as of specific protein–protein interactions. Here using plasma membrane-resident SNARE proteins as model, we show that hydrophobic mismatch between the length of transmembrane domains (TMDs) and the thickness of the lipid membrane suffices to induce clustering of proteins. Even when the TMDs differ in length by only a single residue, hydrophobic mismatch can segregate structurally closely homologous membrane proteins in distinct membrane domains. Domain formation is further fine-tuned by interactions with polyanionic phosphoinositides and homo and heterotypic protein interactions. Our findings demonstrate that hydrophobic mismatch contributes to the structural organization of membranes. PMID:25635869

  18. Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains

    NASA Astrophysics Data System (ADS)

    Milovanovic, Dragomir; Honigmann, Alf; Koike, Seiichi; Göttfert, Fabian; Pähler, Gesa; Junius, Meike; Müllar, Stefan; Diederichsen, Ulf; Janshoff, Andreas; Grubmüller, Helmut; Risselada, Herre J.; Eggeling, Christian; Hell, Stefan W.; van den Bogaart, Geert; Jahn, Reinhard

    2015-01-01

    The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic and ionic interactions with membrane lipids as well as of specific protein-protein interactions. Here using plasma membrane-resident SNARE proteins as model, we show that hydrophobic mismatch between the length of transmembrane domains (TMDs) and the thickness of the lipid membrane suffices to induce clustering of proteins. Even when the TMDs differ in length by only a single residue, hydrophobic mismatch can segregate structurally closely homologous membrane proteins in distinct membrane domains. Domain formation is further fine-tuned by interactions with polyanionic phosphoinositides and homo and heterotypic protein interactions. Our findings demonstrate that hydrophobic mismatch contributes to the structural organization of membranes.

  19. Simulation studies of emittance growth in RMS mismatched beams

    SciTech Connect

    Cucchetti, A.; Wangler, T. ); Reiser, M. )

    1991-01-01

    As shown in a separate paper, a charged-particle beam, whose rms size is not matched when injected into a transport channel or accelerator, has excess energy compared with that of a matched beam. If nonlinear space-charge forces are present and the mismatched beam transforms to a matched equilibrium state, rms-emittance growth will occur. The theory yields formulas for the possible rms-emittance growth, but not for the time it takes to achieve this growth. In this paper we present the results of systematic simulation studies for a mismatched 2-D round beam in an ideal transport channel with continuous linear focusing. Emittance growth rates obtained from the simulations for different amounts of mismatch and initial charge will be presented and the emittance growth will be compared with the theory. 6 refs., 7 figs.

  20. High fitness costs of climate change-induced camouflage mismatch.

    PubMed

    Zimova, Marketa; Mills, L Scott; Nowak, J Joshua

    2016-03-01

    Anthropogenic climate change has created myriad stressors that threaten to cause local extinctions if wild populations fail to adapt to novel conditions. We studied individual and population-level fitness costs of a climate change-induced stressor: camouflage mismatch in seasonally colour molting species confronting decreasing snow cover duration. Based on field measurements of radiocollared snowshoe hares, we found strong selection on coat colour molt phenology, such that animals mismatched with the colour of their background experienced weekly survival decreases up to 7%. In the absence of adaptive response, we show that these mortality costs would result in strong population-level declines by the end of the century. However, natural selection acting on wide individual variation in molt phenology might enable evolutionary adaptation to camouflage mismatch. We conclude that evolutionary rescue will be critical for hares and other colour molting species to keep up with climate change. PMID:26799459

  1. Estimation of channel mismatches in time-interleaved analog-to-digital converters based on fractional delay and sine curve fitting.

    PubMed

    Guo, Lianping; Tian, Shulin; Jiang, Jun

    2015-03-01

    This paper proposes an algorithm to estimate the channel mismatches in time-interleaved analog-to-digital converter (TIADC) based on fractional delay (FD) and sine curve fitting. Choose one channel as the reference channel and apply FD to the output samples of reference channel to obtain the ideal samples of non-reference channels with no mismatches. Based on least square method, the sine curves are adopted to fit the ideal and the actual samples of non-reference channels, and then the mismatch parameters can be estimated by comparing the ideal sine curves and the actual ones. The principle of this algorithm is simple and easily understood. Moreover, its implementation needs no extra circuits, lowering the hardware cost. Simulation results show that the estimation accuracy of this algorithm can be controlled within 2%. Finally, the practicability of this algorithm is verified by the measurement results of channel mismatch errors of a two-channel TIADC prototype. PMID:25832264

  2. Does a helicase activity help mismatch repair in eukaryotes?

    PubMed

    Song, Limin; Yuan, Fenghua; Zhang, Yanbin

    2010-07-01

    Mismatch repair (MMR) corrects innate DNA replication infidelities. Many components of eukaryotic MMR have been identified, the molecular mechanism of MMR has been largely demonstrated, and furthermore the nick-directed MMR reactions have been reconstituted with purified human proteins in vitro. However, some fundamental questions still remain to be answered. One such question is whether a DNA helicase activity is required for MMR in eukaryotes. This short review presents an overview of the interactions between eukaryotic DNA helicases and MMR factors, and suggests a possible mechanism for how DNA helicases may be involved in repair of DNA mismatches. PMID:20552646

  3. Coherent phonon modulation by nanoscale acoustically mismatched interface

    NASA Astrophysics Data System (ADS)

    Yu, Shangjie; Ouyang, Min

    2015-03-01

    Precise engineering of phonon spectrum by material design is essential for in-depth understanding of fundamental physical phenomena as well as new technology breakthrough. When phonons propagate through two different constituents, their mismatched interface can coherently modulate phonon spectrum. In this talk, we will demonstrate the phonon characteristics can be precisely tailored through nanoscale interfacial coupling by investigating acoustically mismatched core-shell hetero-nanostructures with ultrafast pump-probe technique. Coherent phonon coupling between core and shell through their interface has been experimentally revealed, which agrees well with theoretical simulation. This interfacial phonon coupling also represents a unique fingerprint of complex nanostructures.

  4. Constitutional mismatch repair deficiency syndrome: Do we know it?

    PubMed Central

    Ramachandra, C.; Challa, Vasu Reddy; Shetty, Rachan

    2014-01-01

    Constitutional mismatch repair deficiency syndrome is a rare autosomal recessive syndrome caused by homozygous mutations in mismatch repair genes. This is characterized by the childhood onset of brain tumors, colorectal cancers, cutaneous manifestations of neurofibromatosis-1 like café au lait spots, hematological malignancies, and occasionally other rare malignancies. Here, we would like to present a family in which the sibling had glioblastoma, and the present case had acute lymphoblastic lymphoma and colorectal cancer. We would like to present this case because of its rarity and would add to literature. PMID:25400351

  5. Thermal expansion mismatch and oxidation in thermal barrier coatings

    NASA Technical Reports Server (NTRS)

    Chang, G. C.; Phucharoen, W.; Miller, R. A.

    1985-01-01

    Thermal barrier coatings (TBC) for advanced gas turbine blades have been under intensive development during the last several years. This investigation is intended to achieve a clearer understanding of the mechanical behavior of plasma sprayed zirconia-yttria TBCs, involving a nickle-chromium-aluminum bond coat. The near term objectives are to study the stress states in a relatively simple model TBC subjected to steady state thermal loading. The resulting thermal expansion mismatch and oxidation have been primary targets for the study. The finite element approach and the effects of thermal mismatch and oxidation are described. A proposed mechanism for oxidation induced coating failure is also presented.

  6. An Integrated Ransac and Graph Based Mismatch Elimination Approach for Wide-Baseline Image Matching

    NASA Astrophysics Data System (ADS)

    Hasheminasab, M.; Ebadi, H.; Sedaghat, A.

    2015-12-01

    In this paper we propose an integrated approach in order to increase the precision of feature point matching. Many different algorithms have been developed as to optimizing the short-baseline image matching while because of illumination differences and viewpoints changes, wide-baseline image matching is so difficult to handle. Fortunately, the recent developments in the automatic extraction of local invariant features make wide-baseline image matching possible. The matching algorithms which are based on local feature similarity principle, using feature descriptor as to establish correspondence between feature point sets. To date, the most remarkable descriptor is the scale-invariant feature transform (SIFT) descriptor , which is invariant to image rotation and scale, and it remains robust across a substantial range of affine distortion, presence of noise, and changes in illumination. The epipolar constraint based on RANSAC (random sample consensus) method is a conventional model for mismatch elimination, particularly in computer vision. Because only the distance from the epipolar line is considered, there are a few false matches in the selected matching results based on epipolar geometry and RANSAC. Aguilariu et al. proposed Graph Transformation Matching (GTM) algorithm to remove outliers which has some difficulties when the mismatched points surrounded by the same local neighbor structure. In this study to overcome these limitations, which mentioned above, a new three step matching scheme is presented where the SIFT algorithm is used to obtain initial corresponding point sets. In the second step, in order to reduce the outliers, RANSAC algorithm is applied. Finally, to remove the remained mismatches, based on the adjacent K-NN graph, the GTM is implemented. Four different close range image datasets with changes in viewpoint are utilized to evaluate the performance of the proposed method and the experimental results indicate its robustness and capability.

  7. Semantic Processing in Deaf and Hard-of-Hearing Children: Large N400 Mismatch Effects in Brain Responses, Despite Poor Semantic Ability

    PubMed Central

    Kallioinen, Petter; Olofsson, Jonas; Nakeva von Mentzer, Cecilia; Lindgren, Magnus; Ors, Marianne; Sahlén, Birgitta S.; Lyxell, Björn; Engström, Elisabet; Uhlén, Inger

    2016-01-01

    Difficulties in auditory and phonological processing affect semantic processing in speech comprehension for deaf and hard-of-hearing (DHH) children. However, little is known about brain responses related to semantic processing in this group. We investigated event-related potentials (ERPs) in DHH children with cochlear implants (CIs) and/or hearing aids (HAs), and in normally hearing controls (NH). We used a semantic priming task with spoken word primes followed by picture targets. In both DHH children and controls, cortical response differences between matching and mismatching targets revealed a typical N400 effect associated with semantic processing. Children with CI had the largest mismatch response despite poor semantic abilities overall; Children with CI also had the largest ERP differentiation between mismatch types, with small effects in within-category mismatch trials (target from same category as prime) and large effects in between-category mismatch trials (where target is from a different category than prime), compared to matching trials. Children with NH and HA had similar responses to both mismatch types. While the large and differentiated ERP responses in the CI group were unexpected and should be interpreted with caution, the results could reflect less precision in semantic processing among children with CI, or a stronger reliance on predictive processing. PMID:27559320

  8. Semantic Processing in Deaf and Hard-of-Hearing Children: Large N400 Mismatch Effects in Brain Responses, Despite Poor Semantic Ability.

    PubMed

    Kallioinen, Petter; Olofsson, Jonas; Nakeva von Mentzer, Cecilia; Lindgren, Magnus; Ors, Marianne; Sahlén, Birgitta S; Lyxell, Björn; Engström, Elisabet; Uhlén, Inger

    2016-01-01

    Difficulties in auditory and phonological processing affect semantic processing in speech comprehension for deaf and hard-of-hearing (DHH) children. However, little is known about brain responses related to semantic processing in this group. We investigated event-related potentials (ERPs) in DHH children with cochlear implants (CIs) and/or hearing aids (HAs), and in normally hearing controls (NH). We used a semantic priming task with spoken word primes followed by picture targets. In both DHH children and controls, cortical response differences between matching and mismatching targets revealed a typical N400 effect associated with semantic processing. Children with CI had the largest mismatch response despite poor semantic abilities overall; Children with CI also had the largest ERP differentiation between mismatch types, with small effects in within-category mismatch trials (target from same category as prime) and large effects in between-category mismatch trials (where target is from a different category than prime), compared to matching trials. Children with NH and HA had similar responses to both mismatch types. While the large and differentiated ERP responses in the CI group were unexpected and should be interpreted with caution, the results could reflect less precision in semantic processing among children with CI, or a stronger reliance on predictive processing. PMID:27559320

  9. The differential effects of core stabilization exercise regime and conventional physiotherapy regime on postural control parameters during perturbation in patients with movement and control impairment chronic low back pain

    PubMed Central

    2010-01-01

    Background The purpose of the present study was to examine the differential effect of core stability exercise training and conventional physiotherapy regime on altered postural control parameters in patients with chronic low back pain (CLBP). As heterogeneity in CLBP population moderates the effect of intervention on outcomes, in this study, interventions approaches were used based on sub-groups of CLBP. Methods This was an allocation concealed, blinded, sequential and pragmatic control trial. Three groups of participants were investigated during postural perturbations: 1) CLBP patients with movement impairment (n = 15, MI group) randomized to conventional physiotherapy regime 2) fifteen CLBP patients with control impairment randomized to core stability group (CI group) and 3) fifteen healthy controls (HC). Results The MI group did not show any significant changes in postural control parameters after the intervention period however they improved significantly in disability scores and fear avoidance belief questionnaire work score (P < 0.05). The CI group showed significant improvements in Fx, Fz, and My variables (p < 0.013, p < 0.006, and p < 0.002 respectively with larger effect sizes: Hedges's g > 0.8) after 8 weeks of core stability exercises for the adjusted p values. Postural control parameters of HC group were analyzed independently with pre and post postural control parameters of CI and MI group. This revealed the significant improvements in postural control parameters in CI group compared to MI group indicating the specific adaptation to the core stability exercises in CI group. Though the disability scores were reduced significantly in CI and MI groups (p < 0.001), the post intervention scores between groups were not found significant (p < 0.288). Twenty percentage absolute risk reduction in flare-up rates during intervention was found in CI group (95% CI: 0.69-0.98). Conclusions In this study core stability exercise group demonstrated significant

  10. Price and Tax Measures and Illicit Trade in the Framework Convention on Tobacco Control: What We Know and What Research Is Required

    PubMed Central

    2013-01-01

    Introduction: Article 6 of the Framework Convention on Tobacco Control commits Parties to use tax and price policies to reduce tobacco use, whereas Article 15 commits Parties to implement measures to eliminate the illicit trade in tobacco products. This paper identifies research gaps/needs, especially in low- and middle-income countries, which, if adequately addressed, would help in implementing Articles 6 and 15. Methods: Based on a recent comprehensive review on the impact of tax and price on tobacco consumption and a summary of reviews and narratives about the illicit tobacco market, research gaps are identified. Results: Countries have highly diverse research needs, depending on the stage of the tobacco epidemic, previous research and data availability, and making a ranking of research needs infeasible. Broad issues for further research are the following: (1) monitoring tobacco consumption, prices, and taxes, (2) assessing the effectiveness of the tax structure in generating revenue and reducing tobacco use, (3) strengthening the tax administration system in order to reduce tax evasion and tax avoidance, (4) improving our understanding of the political economy of tobacco tax policy, and (5) employing a multidisciplinary approach to assessing the magnitude of illicit tobacco trade. Conclusions: At a technical level, the case for increasing excise taxes to improve public health and increase government revenue is easily made, but the political and policy environment is often not supportive. In order to effectively impact policy, the required approach would typically make use of rigorous economic techniques, and be cognizant of the political economy of raising excise taxes. PMID:22987785

  11. Low-volume application by mist-blower compared with conventional compression sprayer treatment of houses with residual pyrethroid to control the malaria vector Anopheles albimanus in Mexico.

    PubMed

    Villarreal, C; Rodriguez, M H; Bown, D N; Arredondo-Jiménez, J I

    1995-04-01

    Village-scale trials were carried out in southern Mexico to compare the efficacy of indoor-spraying of the pyrethroid insecticide lambda-cyhalothrin applied either as low-volume (LV) aqueous emulsion or as wettable-powder (WP) aqueous suspension for residual control of the principal coastal malaria vector Anopheles albimanus. Three indoor spray rounds were conducted at 3-month intervals using back-pack mist-blowers to apply lambda-cyhalothrin 12.5 mg a.i./m2 by LV, whereas the WP was applied by conventional compression sprayer at a mean rate of 26.5 mg a.i./m2. Both treatments caused mosquito mortality indoors and outdoors (collected inside house curtains) as a result of contact with treated surfaces before and after feeding, but had no significant impact on overall population density of An. albimanus resting indoors or assessed by human bait collections. Contact bioassays showed that WP and LV treatments with lambda-cyhalothrin were effective for 12-20 weeks (> 75% mortality) without causing excito-repellency. Compared to the WP treatment (8 houses/man/day), LV treatment (25 houses/man/day) was more than 3 times quicker per house, potentially saving 68% of labour costs. This is offset, however, by the much lower unit price of a compression sprayer (e.g. Hudson 'X-pert' at US$120) than a mist-blower (e.g. 'Super Jolly' at US$350), and higher running costs for LV applications. It was calculated, therefore, that LV becomes more economical than WP after 18.8 treatments/100 houses/10 men at equivalent rates of application, or after 7.6 spray rounds with half-rate LV applications. PMID:7787228

  12. Evaluation of food additives as alternative or complementary chemicals to conventional fungicides for the control of major postharvest diseases of stone fruit.

    PubMed

    Palou, Lluis; Smilanick, Joseph L; Crisosto, Carlos H

    2009-05-01

    To evaluate potential alternatives to conventional fungicides to control decay, more than 20 food additives and generally regarded as safe compounds were tested at three concentrations in in vivo primary screenings with several cultivars of California peaches, nectarines, and plums that had been artificially inoculated with seven major postharvest pathogens: Monilinia fructicola, Botrytis cinerea, Geotrichum candidum, Alternaria alternata, Penicillium expansum, Mucor piriformis, and Rhizopus stolonifer. Overall, the best compounds were 200 mM potassium sorbate (PS), 200 mM sodium benzoate (SB), 200 mM sodium sorbate, 100 mM 2-deoxy-D-glucose, 400 mM sodium carbonate, and 250 mM potassium carbonate. Sodium and ammonium molybdates, acid lactic, and hydrogen peroxide were somewhat effective but were phytotoxic to fruit skin tissues. However, the best compounds lacked effectiveness and persistence when tested against brown rot in small-scale trials of 60-s dips in aqueous solutions at ambient temperatures; PS and SB reduced brown rot incidence by less than 40%. Rinsing treated fruit with tap water reduced the efficacy of the compounds by up to 30%. In contrast, heating the solutions to 55 or 60 degrees C significantly increased treatment efficacy. Brown rot incidence and severity were reduced by 35 and 25%, respectively, on PS-treated peaches after 7 days of incubation at 20 degrees C. However, treatment efficacy was not superior to that with water alone at these temperatures. In semicommercial trials, mixtures of fludioxonil with PS, SB, or 2-deoxy-D-glucose applied as fruit coatings on a packing line were not synergistic in their effect on brown rot, gray mold, and sour rot. PMID:19517732

  13. Xerostomia and quality of life after intensity-modulated radiotherapy vs. conventional radiotherapy for early-stage nasopharyngeal carcinoma: Initial report on a randomized controlled clinical trial

    SciTech Connect

    Pow, Edmond; Kwong, Dora; McMillan, Anne S. . E-mail: annemcmillan@hku.hk; Wong, May; Sham, Jonathan; Leung, Lucullus; Leung, W. Keung

    2006-11-15

    Purpose: To compare directly the effect of intensity-modulated radiotherapy (IMRT) vs. conventional radiotherapy (CRT) on salivary flow and quality of life (QoL) in patients with early-stage nasopharyngeal carcinoma (NPC). Methods and Materials: Fifty-one patients with T2, N0/N1, M0 NPC took part in a randomized controlled clinical study and received IMRT or CRT. Stimulated whole (SWS) and parotid (SPS) saliva flow were measured and Medical Outcomes Short Form 36 (SF-36), European Organization for Research and Treatment of Cancer (EORTC) core quetionnaire, and EORTC head-and-neck module (QLQ-H and N35) were completed at baseline and 2, 6, and 12 months after radiotherapy. Results: Forty-six patients (88%) were in disease remission 12 months after radiotherapy. At 12 months postradiotherapy, 12 (50.0%) and 20 patients (83.3%) in the IMRT group had recovered at least 25% of preradiotherapy SWS and SPS flow respectively, compared with 1 (4.8%) and 2 patients (9.5%), respectively, in the CRT group. Global health scores showed continuous improvement in QoL after both treatments (p < 0.001). However, after 12 months subscale scores for role-physical, bodily pain, and physical function were significantly higher in the IMRT group, indicating a better condition (p < 0.05). Dry mouth and sticky saliva were problems in both groups 2 months after treatment. In the IMRT group, there was consistent improvement over time with xerostomia-related symptoms significantly less common than in the CRT group at 12 months postradiotherapy. Conclusions: IMRT was significantly better than CRT in terms of parotid sparing and improved QoL for early-stage disease. The findings support the case for assessment of health-related QoL in relation to head-and-neck cancer using a site-specific approach.

  14. Is Consumer Response to Plain/Standardised Tobacco Packaging Consistent with Framework Convention on Tobacco Control Guidelines? A Systematic Review of Quantitative Studies

    PubMed Central

    Stead, Martine; Moodie, Crawford; Angus, Kathryn; Bauld, Linda; McNeill, Ann; Thomas, James; Hastings, Gerard; Hinds, Kate; O'Mara-Eves, Alison; Kwan, Irene; Purves, Richard I.; Bryce, Stuart L.

    2013-01-01

    Background and Objectives Standardised or ‘plain’ tobacco packaging was introduced in Australia in December 2012 and is currently being considered in other countries. The primary objective of this systematic review was to locate, assess and synthesise published and grey literature relating to the potential impacts of standardised tobacco packaging as proposed by the guidelines for the international Framework Convention on Tobacco Control: reduced appeal, increased salience and effectiveness of health warnings, and more accurate perceptions of product strength and harm. Methods Electronic databases were searched and researchers in the field were contacted to identify studies. Eligible studies were published or unpublished primary research of any design, issued since 1980 and concerning tobacco packaging. Twenty-five quantitative studies reported relevant outcomes and met the inclusion criteria. A narrative synthesis was conducted. Results Studies that explored the impact of package design on appeal consistently found that standardised packaging reduced the appeal of cigarettes and smoking, and was associated with perceived lower quality, poorer taste and less desirable smoker identities. Although findings were mixed, standardised packs tended to increase the salience and effectiveness of health warnings in terms of recall, attention, believability and seriousness, with effects being mediated by the warning size, type and position on pack. Pack colour was found to influence perceptions of product harm and strength, with darker coloured standardised packs generally perceived as containing stronger tasting and more harmful cigarettes than fully branded packs; lighter coloured standardised packs suggested weaker and less harmful cigarettes. Findings were largely consistent, irrespective of location and sample. Conclusions The evidence strongly suggests that standardised packaging will reduce the appeal of packaging and of smoking in general; that it will go some way

  15. [Ru(Me4phen)2dppz](2+), a Light Switch for DNA Mismatches.

    PubMed

    Boynton, Adam N; Marcélis, Lionel; Barton, Jacqueline K

    2016-04-20

    [Ru(Me4phen)2dppz](2+) serves as a luminescent "light switch" for single base mismatches in DNA. The preferential luminescence enhancement observed with mismatches results from two factors: (i) the complex possesses a 26-fold higher binding affinity toward the mismatch compared to well-matched base pairs, and (ii) the excited state emission lifetime of the ruthenium bound to the DNA mismatch is 160 ns versus 35 ns when bound to a matched site. Results indicate that the complex binds to the mismatch through a metalloinsertion binding mode. Cu(phen)2(2+) quenching experiments show that the complex binds to the mismatch from the minor groove, characteristic of metalloinsertion. Additionally, the luminescence intensity of the complex with DNA containing single base mismatches correlates with the thermodynamic destabilization of the mismatch, also consistent with binding through metalloinsertion. This complex represents a potentially new early cancer diagnostic for detecting deficiencies in mismatch repair. PMID:27068529

  16. Highly Mismatched Alloys for Intermediate Band Solar Cells

    SciTech Connect

    Walukiewicz, W.; Yu, K.M.; Wu, J.; Ager III, J.W.; Shan, W.; Scrapulla, M.A.; Dubon, O.D.; Becla, P.

    2005-03-21

    It has long been recognized that the introduction of a narrow band of states in a semiconductor band gap could be used to achieve improved power conversion efficiency in semiconductor-based solar cells. The intermediate band would serve as a ''stepping stone'' for photons of different energy to excite electrons from the valence to the conduction band. An important advantage of this design is that it requires formation of only a single p-n junction, which is a crucial simplification in comparison to multijunction solar cells. A detailed balance analysis predicts a limiting efficiency of more than 50% for an optimized, single intermediate band solar cell. This is higher than the efficiency of an optimized two junction solar cell. Using ion beam implantation and pulsed laser melting we have synthesized Zn{sub 1-y}Mn{sub y}O{sub x}Te{sub 1-x} alloys with x<0.03. These highly mismatched alloys have a unique electronic structure with a narrow oxygen-derived intermediate band. The width and the location of the band is described by the Band Anticrossing model and can be varied by controlling the oxygen content. This provides a unique opportunity to optimize the absorption of solar photons for best solar cell performance. We have carried out systematic studies of the effects of the intermediate band on the optical and electrical properties of Zn{sub 1-y}Mn{sub y}O{sub x}Te{sub 1-x} alloys. We observe an extension of the photovoltaic response towards lower photon energies, which is a clear indication of optical transitions from the valence to the intermediate band.

  17. Effects of donor/recipient human leukocyte antigen mismatch on human cytomegalovirus replication following liver transplantation

    PubMed Central

    Aldridge, RW; Mattes, FM; Rolando, N; Rolles, K; Smith, C; Shirling, G; Atkinson, C; Burroughs, AK; Milne, RSB; Emery, VC; Griffiths, PD

    2015-01-01

    Background Natural immunity against cytomegalovirus (CMV) can control virus replication after solid organ transplantation; however, it is not known which components of the adaptive immune system mediate this protection. We investigated whether this protection requires human leukocyte antigen (HLA) matching between donor and recipient by exploiting the fact that, unlike transplantation of other solid organs, liver transplantation does not require HLA matching, but some donor and recipient pairs may nevertheless be matched by chance. Methods To further investigate this immune control, we determined whether chance HLA matching between donor (D) and recipient (R) in liver transplants affected a range of viral replication parameters. Results In total, 274 liver transplant recipients were stratified according to matches at the HLA A, HLA B, and HLA DR loci. The incidence of CMV viremia, kinetics of replication, and peak viral load were similar between the HLA matched and mismatched patients in the D+/R+ and D−/R+ transplant groups. D+/R− transplants with 1 or 2 mismatches at the HLA DR locus had a higher incidence of CMV viremia >3000 genomes/mL blood compared to patients matched at this locus (78% vs. 17%; P = 0.01). Evidence was seen that matching at the HLA A locus had a small effect on peak viral loads in D+/R− patients, with median peak loads of 3540 and 14,706 genomes/mL in the 0 and combined (1 and 2) mismatch groups, respectively (P = 0.03). Conclusion Overall, our data indicate that, in the setting of liver transplantation, prevention of CMV infection and control of CMV replication by adaptive immunity is minimally influenced by HLA matching of the donor and recipient. Our data raise questions about immune control of CMV in the liver and also about the cells in which the virus is amplified to give rise to CMV viremia. PMID:25572799

  18. 15 CFR 742.18 - Chemical Weapons Convention (CWC or Convention).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Chemical Weapons Convention (CWC or... Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE EXPORT ADMINISTRATION REGULATIONS CONTROL POLICY-CCL BASED CONTROLS § 742.18 Chemical Weapons Convention (CWC or Convention)....

  19. 15 CFR 742.18 - Chemical Weapons Convention (CWC or Convention).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Chemical Weapons Convention (CWC or... Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE EXPORT ADMINISTRATION REGULATIONS CONTROL POLICY-CCL BASED CONTROLS § 742.18 Chemical Weapons Convention (CWC or Convention)....

  20. 15 CFR 742.18 - Chemical Weapons Convention (CWC or Convention).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Chemical Weapons Convention (CWC or... Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE EXPORT ADMINISTRATION REGULATIONS CONTROL POLICY-CCL BASED CONTROLS § 742.18 Chemical Weapons Convention (CWC or Convention)....

  1. 15 CFR 742.18 - Chemical Weapons Convention (CWC or Convention).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Chemical Weapons Convention (CWC or... Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE EXPORT ADMINISTRATION REGULATIONS CONTROL POLICY-CCL BASED CONTROLS § 742.18 Chemical Weapons Convention (CWC or Convention)....

  2. 15 CFR 742.18 - Chemical Weapons Convention (CWC or Convention).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Chemical Weapons Convention (CWC or... Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE EXPORT ADMINISTRATION REGULATIONS CONTROL POLICY-CCL BASED CONTROLS § 742.18 Chemical Weapons Convention (CWC or Convention)....

  3. Supply and Demand Mismatches in Training: Can Anything Be Done?

    ERIC Educational Resources Information Center

    Castro, Claudio de Moura; de Andrade, Antonio Cabral

    1990-01-01

    Vocational training often fails to provide what employers need and students want. To correct supply/demand mismatches requires improving feedback from employers, increasing the flow of information, bringing schools closer to businesses, rewarding institutions for successful employment of graduates, and providing incentives for entrepreneurs. (SK)

  4. Minority Students and Research Universities: How to Overcome the "Mismatch"

    ERIC Educational Resources Information Center

    Tapia, Richard A.

    2009-01-01

    A controversial theory much in the news lately claims that affirmative action is often unfair to the very students it is intended to help. Called the "mismatch" theory, it suggests that underrepresented minority students are more likely to leave science, math, and engineering when, because of affirmative action, they attend colleges for which they…

  5. Skills Mismatch among University Graduates in the Nigeria Labor Market

    ERIC Educational Resources Information Center

    Pitan, Oluyomi S.; Adedeji, S. O.

    2012-01-01

    University graduates in Nigeria have been reported to be poorly prepared for work in recent years. This has implications on the relevance of university education, the employability and productivity of university graduates. One of the reasons suggested for this condition by previous studies was skill mismatch--a situation where there is a disparity…

  6. Coded aperture design in mismatched compressive spectral imaging.

    PubMed

    Galvis, Laura; Arguello, Henry; Arce, Gonzalo R

    2015-11-20

    Compressive spectral imaging (CSI) senses a scene by using two-dimensional coded projections such that the number of measurements is far less than that used in spectral scanning-type instruments. An architecture that efficiently implements CSI is the coded aperture snapshot spectral imager (CASSI). A physical limitation of the CASSI is the system resolution, which is determined by the lowest resolution element used in the detector and the coded aperture. Although the final resolution of the system is usually given by the detector, in the CASSI, for instance, the use of a low resolution coded aperture implemented using a digital micromirror device (DMD), which induces the grouping of pixels in superpixels in the detector, is decisive to the final resolution. The mismatch occurs by the differences in the pitch size of the DMD mirrors and focal plane array (FPA) pixels. A traditional solution to this mismatch consists of grouping several pixels in square features, which subutilizes the DMD and the detector resolution and, therefore, reduces the spatial and spectral resolution of the reconstructed spectral images. This paper presents a model for CASSI which admits the mismatch and permits exploiting the maximum resolution of the coding element and the FPA sensor. A super-resolution algorithm and a synthetic coded aperture are developed in order to solve the mismatch. The mathematical models are verified using a real implementation of CASSI. The results of the experiments show a significant gain in spatial and spectral imaging quality over the traditional grouping pixel technique. PMID:26836551

  7. A mishmash of methods for mitigating the model mismatch mess

    NASA Astrophysics Data System (ADS)

    Ker, Andrew D.; Pevný, Tomáš

    2014-02-01

    The model mismatch problem occurs in steganalysis when a binary classifier is trained on objects from one cover source and tested on another: an example of domain adaptation. It is highly realistic because a steganalyst would rarely have access to much or any training data from their opponent, and its consequences can be devastating to classifier accuracy. This paper presents an in-depth study of one particular instance of model mismatch, in a set of images from Flickr using one fixed steganography and steganalysis method, attempting to separate different effects of mismatch in feature space and find methods of mitigation where possible. We also propose new benchmarks for accuracy, which are more appropriate than mean error rates when there are multiple actors and multiple images, and consider the case of 3-valued detectors which also output `don't know'. This pilot study demonstrates that some simple feature-centering and ensemble methods can reduce the mismatch penalty considerably, but not completely remove it.

  8. Highly Mismatched, Dislocation-Free SiGe/Si Heterostructures.

    PubMed

    Isa, Fabio; Salvalaglio, Marco; Dasilva, Yadira Arroyo Rojas; Meduňa, Mojmír; Barget, Michael; Jung, Arik; Kreiliger, Thomas; Isella, Giovanni; Erni, Rolf; Pezzoli, Fabio; Bonera, Emiliano; Niedermann, Philippe; Gröning, Pierangelo; Montalenti, Francesco; von Känel, Hans

    2016-02-01

    Defect-free mismatched heterostructures on Si substrates are produced by an innovative strategy. The strain relaxation is engineered to occur elastically rather than plastically by combining suitable substrate patterning and vertical crystal growth with compositional grading. Its validity is proven both experimentally and theoretically for the pivotal case of SiGe/Si(001). PMID:26829168

  9. ABO-Mismatched Allogeneic Hematopoietic Stem Cell Transplantation

    PubMed Central

    Worel, Nina

    2016-01-01

    Summary Allogeneic hematopoietic stem cell transplantation (HSCT) is a curative option for a variety of malignant and non-malignant hematological and congenital diseases. Due to the fact that the human leukocyte antigen system is inherited independently of the blood group system, approximately 40-50% of all HSCTs are performed across the ABO blood group barrier. The expected immune-hematological consequences after transplantation of an ABO-mismatched stem cell graft are immediate and delayed hemolytic complications due to presence of isohemagglutinins or passenger lymphocyte syndrome. The risks of these complications can partially be prevented by graft manipulation and appropriate transfusion support. Dependent on the kind of ABO mismatch, different effects on engraftment have been observed, e.g. delayed red blood cell recovery and pure red cell aplasia. Data on incidence of acute graft-versus-host disease (GVHD), non-relapse mortality, relapse, and overall survival are inconsistent as most studies include limited patient numbers, various graft sources, and different conditioning and GVHD prophylaxis regimens. This makes it difficult to detect a consistent effect of ABO-mismatched transplantation in the literature. However, knowledge of expectable complications and close monitoring of patients helps to detect problems early and to treat patients efficiently, thus reducing the number of fatal or life-threatening events caused by ABO-mismatched HSCT. PMID:27022317

  10. Expectations and Outcomes of Vocational Education: Match or Mismatch.

    ERIC Educational Resources Information Center

    Lotto, Linda S.

    1986-01-01

    This article provides an initial look at the matches and mismatches between expectations held for vocational education programs and empirically observed outcomes. Three types of claims for vocational education were compared with recent research. The article concludes with recommendations for improving the alignment between expectations and…

  11. Mismatch of Vocational Graduates: What Penalty on French Labour Market?

    ERIC Educational Resources Information Center

    Beduwe, Catherine; Giret, Jean-Francois

    2011-01-01

    This study explores individual effects of educational mismatch on wages, job satisfaction and on-the-job-search on French labour market. We distinguish between horizontal matches (job matches with field of studies) and vertical matches (job matches the level of qualification) on the one hand and skills matches (worker's assessment) on the other…

  12. Modeling of channel mismatch in time-interleaved SAR ADC

    NASA Astrophysics Data System (ADS)

    Dengquan, Li; Liang, Zhang; Zhangming, Zhu; Yintang, Yang

    2015-09-01

    In a time-interleaved analog-to-digital converter (TI ADC), several individual ADCs operate in parallel to achieve a higher sampling rate. Low power consumption as well as good linearity can be obtained by applying successive approximation register (SAR) converters as sub-channel ADCs. In spite of the advantages, this structure suffers from three mismatches, which are offset mismatch, gain mismatch, and time skew. This paper focuses on a TI SAR ADC with a number of channels. The mismatch effects in the frequency domain are analyzed and the derived close form formulas are verified based on Matlab. In addition, we clarify that the standard deviation of DNL and INL of an M-channel TI ADC is reduced by a factor of \\sqrt M compared to a single channel ADC. The formulas can be used to derive the corresponding requirements when designing a TI ADC. Our analysis process is able to inform the study of calibration algorithms. Project supported by the National Natural Science Foundation of China (Nos. 61234002, 61322405, 61306044, 61376033) and the National High-Tech Program of China (No. 2013AA014103).

  13. Avalanching mutations in biallelic mismatch repair deficiency syndrome.

    PubMed

    Waterfall, Joshua J; Meltzer, Paul S

    2015-03-01

    Tumors from pediatric patients generally contain relatively few somatic mutations. A new study reports a striking exception in individuals in whom biallelic germline deficiency for mismatch repair is compounded by somatic loss of function in DNA proofreading polymerases, resulting in 'ultra-hypermutated' malignant brain tumors. PMID:25711864

  14. Educational Mismatch and Spatial Flexibility in Italian Local Labour Markets

    ERIC Educational Resources Information Center

    Croce, Giuseppe; Ghignoni, Emanuela

    2015-01-01

    According to recent literature, this paper highlights the relevance of spatial mobility as an explanatory factor of the individual risk of job-education mismatch. To investigate this causal link, we use individual information about daily home-to-work commuting time and choices to relocate in a different local area to get a job. Our model takes…

  15. Comparison of magnetic wire navigation with the conventional wire technique for percutaneous coronary intervention of chronic total occlusions: a randomised, controlled study.

    PubMed

    Roth, Christian; Berger, Rudolf; Scherzer, Sabine; Krenn, Lisa; Gangl, Clemens; Dalos, Daniel; Delle-Karth, Georg; Neunteufl, Thomas

    2016-08-01

    Wire crossing of a chronic total coronary occlusion (CTO) is time consuming and limited by the amount of contrast agent and time of radiation exposure. Magnetic wire navigation (MWN) might accelerate wire crossing by maintaining a coaxial vessel orientation. This study compares MWN with the conventional approach for recanalization of CTOs. Forty symptomatic patients with CTO were randomised to MWN (n = 20) or conventional approach (n = 20) for antegrade crossing of the occlusion. In the intention-to-treat analysis, MWN showed a shorter crossing time (412 versus 1131 s; p = 0.001), and, consequently, lower usage of contrast agent (primary endpoint 42 versus 116 ml; p = 0.01), and lower radiation exposure (dose-area product: 29 versus 80 Gy*cm(2); p = 0.002) during wire crossing compared to the conventional approach. Accordingly, in the per-protocol analysis, the wire-crossing rate was, in trend, higher using the conventional approach (17 of 31) compared to MWN (9 of 28; p = 0.08). The use of MWN for revascularisation of CTOs is feasible and reduces crossing time, use of contrast agent, and radiation exposure. However, due to a broader selection of wires, the conventional approach enables wire crossing in cases failed by MWN and seems to be the more successful choice. PMID:26369660

  16. State-of-art neuroanatomical target analysis of high-definition and conventional tDCS montages used for migraine and pain control.

    PubMed

    DaSilva, Alexandre F; Truong, Dennis Q; DosSantos, Marcos F; Toback, Rebecca L; Datta, Abhishek; Bikson, Marom

    2015-01-01

    Although transcranial direct current stimulation (tDCS) studies promise to modulate cortical regions associated with pain, the electric current produced usually spreads beyond the area of the electrodes' placement. Using a forward-model analysis, this study compared the neuroanatomic location and strength of the predicted electric current peaks, at cortical and subcortical levels, induced by conventional and High-Definition-tDCS (HD-tDCS) montages developed for migraine and other chronic pain disorders. The electrodes were positioned in accordance with the 10-20 or 10-10 electroencephalogram (EEG) landmarks: motor cortex-supraorbital (M1-SO, anode and cathode over C3 and Fp2, respectively), dorsolateral prefrontal cortex (PFC) bilateral (DLPFC, anode over F3, cathode over F4), vertex-occipital cortex (anode over Cz and cathode over Oz), HD-tDCS 4 × 1 (one anode on C3, and four cathodes over Cz, F3, T7, and P3) and HD-tDCS 2 × 2 (two anodes over C3/C5 and two cathodes over FC3/FC5). M1-SO produced a large current flow in the PFC. Peaks of current flow also occurred in deeper brain structures, such as the cingulate cortex, insula, thalamus and brainstem. The same structures received significant amount of current with Cz-Oz and DLPFC tDCS. However, there were differences in the current flow to outer cortical regions. The visual cortex, cingulate and thalamus received the majority of the current flow with the Cz-Oz, while the anterior parts of the superior and middle frontal gyri displayed an intense amount of current with DLPFC montage. HD-tDCS montages enhanced the focality, producing peaks of current in subcortical areas at negligible levels. This study provides novel information regarding the neuroanatomical distribution and strength of the electric current using several tDCS montages applied for migraine and pain control. Such information may help clinicians and researchers in deciding the most appropriate tDCS montage to treat each pain disorder. PMID:26236199

  17. State-of-art neuroanatomical target analysis of high-definition and conventional tDCS montages used for migraine and pain control

    PubMed Central

    DaSilva, Alexandre F.; Truong, Dennis Q.; DosSantos, Marcos F.; Toback, Rebecca L.; Datta, Abhishek; Bikson, Marom

    2015-01-01

    Although transcranial direct current stimulation (tDCS) studies promise to modulate cortical regions associated with pain, the electric current produced usually spreads beyond the area of the electrodes’ placement. Using a forward-model analysis, this study compared the neuroanatomic location and strength of the predicted electric current peaks, at cortical and subcortical levels, induced by conventional and High-Definition-tDCS (HD-tDCS) montages developed for migraine and other chronic pain disorders. The electrodes were positioned in accordance with the 10–20 or 10–10 electroencephalogram (EEG) landmarks: motor cortex-supraorbital (M1-SO, anode and cathode over C3 and Fp2, respectively), dorsolateral prefrontal cortex (PFC) bilateral (DLPFC, anode over F3, cathode over F4), vertex-occipital cortex (anode over Cz and cathode over Oz), HD-tDCS 4 × 1 (one anode on C3, and four cathodes over Cz, F3, T7, and P3) and HD-tDCS 2 × 2 (two anodes over C3/C5 and two cathodes over FC3/FC5). M1-SO produced a large current flow in the PFC. Peaks of current flow also occurred in deeper brain structures, such as the cingulate cortex, insula, thalamus and brainstem. The same structures received significant amount of current with Cz-Oz and DLPFC tDCS. However, there were differences in the current flow to outer cortical regions. The visual cortex, cingulate and thalamus received the majority of the current flow with the Cz-Oz, while the anterior parts of the superior and middle frontal gyri displayed an intense amount of current with DLPFC montage. HD-tDCS montages enhanced the focality, producing peaks of current in subcortical areas at negligible levels. This study provides novel information regarding the neuroanatomical distribution and strength of the electric current using several tDCS montages applied for migraine and pain control. Such information may help clinicians and researchers in deciding the most appropriate tDCS montage to treat each pain disorder. PMID

  18. Human DNA Polymerase Kappa Encircles DNA: Implicatins for Mismatch Extension and Lesion Bypass

    SciTech Connect

    Lone,S.; Townson, S.; Uljon, S.; Johnson, R.; Brahma, A.; Nair, D.; Prakash, S.; Prakash, L.; Aggarwal, A.

    2007-01-01

    Human DNA polymerase (Pol ) is a proficient extender of mispaired primer termini on undamaged DNAs and is implicated in the extension step of lesion bypass. We present here the structure of Pol catalytic core in ternary complex with DNA and an incoming nucleotide. The structure reveals encirclement of the DNA by a unique 'N-clasp' at the N terminus of Pol , which augments the conventional right-handed grip on the DNA by the palm, fingers, and thumb domains and the PAD and provides additional thermodynamic stability. The structure also reveals an active-site cleft that is constrained by the close apposition of the N-clasp and the fingers domain, and therefore can accommodate only a single Watson-Crick base pair. Together, DNA encirclement and other structural features help explain Pol 's ability to extend mismatches and to promote replication through various minor groove DNA lesions, by extending from the nucleotide incorporated opposite the lesion by another polymerase.

  19. Functional Complex Point-Defect Structure in a Huge-Size-Mismatch System

    NASA Astrophysics Data System (ADS)

    Ishikawa, Ryo; Shibata, Naoya; Oba, Fumiyasu; Taniguchi, Takashi; Findlay, Scott D.; Tanaka, Isao; Ikuhara, Yuichi

    2013-02-01

    Cubic boron nitride is a promising system for photonics and optoelectronics. Determining the inclusion mechanisms for dopants with a large size mismatch, such as luminous rare-earth elements, is prerequisite to understanding their functional properties and to effective doping control. Combining evidence from subangstrom resolution scanning transmission electron microscopy, imaging simulations, and first-principles calculations, we show that cationic Ce3+ single dopants are not located at cationic B sites but rather at anionic N sites surrounded by B-site vacancies.

  20. Recent advances on the use of natural and safe alternatives to conventional methods to control postharvest gray mold of table grapes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Gray mold, caused by Botrytis cinerea, is the main postharvest decay of table grapes. It can develop in the vineyard and spread rapidly among berries after harvest, during long distant transport, cold storage and shelf life. In conventional agriculture, bunches are sprayed with fungicides after flow...

  1. Change your conventional practice of qRT-PCR: A simple robust quality control standard for yeast mRNA quantification analysis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Real time quantitative reverse transcription polymerase chain reaction (qRT-PCR) has been realized to be the assay of choice among available techniques for mRNA quantification analysis. For conventional practice, housekeeping genes have been applied as internal reference for data normalization and a...

  2. Coherent piezoelectric strain transfer to thick epitaxial ferromagnetic films with large lattice mismatch.

    PubMed

    Kim, Jang-Yong; Yao, Lide; van Dijken, Sebastiaan

    2013-02-27

    Strain control of epitaxial films using piezoelectric substrates has recently attracted significant scientific interest. Despite its potential as a powerful test bed for strain-related physical phenomena and strain-driven electronic, magnetic, and optical technologies, detailed studies on the efficiency and uniformity of piezoelectric strain transfer are scarce. Here, we demonstrate that full and uniform piezoelectric strain transfer to epitaxial films is not limited to systems with small lattice mismatch or limited film thickness. Detailed transmission electron microscopy (TEM) and x-ray diffraction (XRD) measurements of 100 nm thick CoFe(2)O(4) and La(2/3)Sr(1/3)MnO(3) epitaxial films on piezoelectric 0.72Pb(Mg(1/3)Nb(2/3))O(3)-0.28PbTiO(3) substrates (+4.3% and -3.8% lattice mismatch) indicate that misfit dislocations near the interface do not hamper the transfer of piezoelectric strain. Instead, the epitaxial magnetic oxide films and PMN-PT substrates are strained coherently and their lattice parameters change linearly as a function of applied electric field when their remnant growth-induced strain state is negligible. As a result, ferromagnetic properties such as the coercive field, saturation magnetization, and Curie temperature can be reversibly tuned by electrical means. The observation of efficient piezoelectric strain transfer in large-mismatch heteroepitaxial structures opens up new possibilities for the engineering of strain-controlled physical properties in a broad class of hybrid material systems. PMID:23370268

  3. Mismatch Responses to Lexical Tone, Initial Consonant, and Vowel in Mandarin-Speaking Preschoolers

    ERIC Educational Resources Information Center

    Lee, Chia-Ying; Yen, Huei-ling; Yeh, Pei-wen; Lin, Wan-Hsuan; Cheng, Ying-Ying; Tzeng, Yu-Lin; Wu, Hsin-Chi

    2012-01-01

    The present study investigates how age, phonological saliency, and deviance size affect the presence of mismatch negativity (MMN) and positive mismatch response (P-MMR). This work measured the auditory mismatch responses to Mandarin lexical tones, initial consonants, and vowels in 4- to 6-year-old preschoolers using the multiple-deviant oddball…

  4. Formal Education, Mismatch and Wages after Transition: Assessing the Impact of Unobserved Heterogeneity Using Matching Estimators

    ERIC Educational Resources Information Center

    Lamo, Ana; Messina, Julian

    2010-01-01

    This paper studies the incidence and consequences of the mismatch between formal education and the educational requirements of jobs in Estonia during the years 1997-2003. We find large wage penalties associated with the phenomenon of educational mismatch. Moreover, the incidence and wage penalty of mismatches increase with age. This suggests that…

  5. Compensation of front-end IQ-mismatch in coherent optical receiver

    NASA Astrophysics Data System (ADS)

    Chang, Sun Hyok; Chung, Hwan Seok; Kim, Kwangjoon

    2011-03-01

    Impacts of in-phase/quadrature-mismatch (IQ-mismatch) in polarization division multiplexed-16 level quadrature amplitude modulation (PDM-16QAM) coherent optical receiver are investigated and the IQ-mismatch compensation by digital signal processing is examined. All the results are compared with those of PDM-quadrature phase shift keying (QPSK) receiver.

  6. Interplay between quantum confinement and dielectric mismatch for ultrashallow dopants

    NASA Astrophysics Data System (ADS)

    Mol, J. A.; Salfi, J.; Miwa, J. A.; Simmons, M. Y.; Rogge, S.

    2013-06-01

    Understanding the electronic properties of dopants near an interface is a critical challenge for nanoscale devices. We have determined the effect of dielectric mismatch and quantum confinement on the ionization energy of individual acceptors beneath a hydrogen passivated silicon (100) surface. While dielectric mismatch between the vacuum and the silicon at the interface results in an image charge which enhances the binding energy of subsurface acceptors, quantum confinement is shown to reduce the binding energy. Using scanning tunneling spectroscopy we measure resonant transport through the localized states of individual acceptors. Thermal broadening of the conductance peaks provides a direct measure for the absolute energy scale. Our data unambiguously demonstrates that these two independent effects compete with the result that the ionization energy is less than 5 meV lower than the bulk value for acceptors less than a Bohr radius from the interface.

  7. Enhanced densification of metal powders by transformation-mismatch plasticity

    SciTech Connect

    Schuh, C.; Noel, P.; Dunand, D.C.

    2000-05-11

    The densification of titanium powders is investigated in uniaxial die pressing experiments carried out isothermally at 980 C (in the {beta}-field of titanium) and during thermal cycling between 860 and 980 C (about the {alpha}/{beta} phase transformation of titanium). Thermal cycling is found to enhance densification kinetics through the emergence of transformation-mismatch plasticity (the mechanism responsible for transformation superplasticity) as a densification mechanism. The isothermal hot-pressing data compare favorably with existing models of powder densification, and these models are successfully adapted to the case of transformation-mismatch plasticity during thermal cycling. Similar conclusions are reached for the densification of titanium powders containing 1, 5, or 10 vol.% ZrO{sub 2} particles. However, the addition of ZrO{sub 2} hinders densification by dissolving in the titanium matrix during the hot-pressing procedure.

  8. Experimental study of proton beam halo in mismatched beams

    SciTech Connect

    Allen, C. K.; Chan, K. D.; Colestock, P. L. ,; Garnett, R. W.; Gilpatrick, J. D.; Qiang, J.; Lysenko, W. P.; Smith, H. V.; Schneider, J. D.; Sheffield, R. L.; Wangler, Thomas P.,; Schulze, M. E.; Crandall, K. R.

    2002-01-01

    We report measurements of transverse beam-halo formation in mismatched proton beams in a 52-quadrupole FODO-transport channel following the 6.7 MeV RFQ at the Low-Energy Demonstration Accelerator (LEDA) at Los Alamos. Beam profiles in both transverse planes were measured using a new diagnostic device that consists of a movable carbon filament for measurement of the beam core, and scraper plates for measurement of the outer part of the distributions. The initial results indicate a surprisingly strong growth rate of the rms emittance even for the modest space-charge tune depressions of the experiment. Our results are consistent with the complete transfer of free energy of the mismatched beams into emittance growth within 10 envelope oscillations for both the breathing and the quadrupole modes.

  9. A Computational Model for Biomechanical Effects of Arterial Compliance Mismatch

    PubMed Central

    He, Fan; Hua, Lu; Gao, Li-jian

    2015-01-01

    Background. Compliance mismatch is a negative factor and it needs to be considered in arterial bypass grafting. Objective. A computational model was employed to investigate the effects of arterial compliance mismatch on blood flow, wall stress, and deformation. Methods. The unsteady blood flow was assumed to be laminar, Newtonian, viscous, and incompressible. The vessel wall was assumed to be linear elastic, isotropic, and incompressible. The fluid-wall interaction scheme was constructed using the finite element method. Results. The results show that there are identical wall shear stress waveforms, wall stress, and strain waveforms at different locations. The comparison of the results demonstrates that wall shear stresses and wall strains are higher while wall stresses are lower at the more compliant section. The differences promote the probability of intimal thickening at some locations. Conclusions. The model is effective and gives satisfactory results. It could be extended to all kinds of arteries with complicated geometrical and material factors. PMID:27019580

  10. Effects of butter from mountain-pasture grazing cows on risk markers of the metabolic syndrome compared with conventional Danish butter: a randomized controlled study

    PubMed Central

    2013-01-01

    Background There is considerable interest in dairy products from low-input systems, such as mountain-pasture grazing cows, because these products are believed to be healthier than products from high-input conventional systems. This may be due to a higher content of bioactive components, such as phytanic acid, a PPAR-agonist derived from chlorophyll. However, the effects of such products on human health have been poorly investigated. Objective To compare the effect of milk-fat from mountain-pasture grazing cows (G) and conventionally fed cows (C) on risk markers of the metabolic syndrome. Design In a double-blind, randomized, 12-week, parallel intervention study, 38 healthy subjects replaced part of their habitual dietary fat intake with 39 g fat from test butter made from milk from mountain-pasture grazing cows or from cows fed conventional winter fodder. Glucose-tolerance and circulating risk markers were analysed before and after the intervention. Results No differences in blood lipids, lipoproteins, hsCRP, insulin, glucose or glucose-tolerance were observed. Interestingly, strong correlations between phytanic acid at baseline and total (P<0.0001) and LDL cholesterol (P=0.0001) were observed. Conclusions Lack of effects on blood lipids and inflammation indicates that dairy products from mountain-pasture grazing cows are not healthier than products from high-input conventional systems. Considering the strong correlation between LDL cholesterol and phytanic acid at baseline, it may be suggested that phytanic acid increases total and LDL cholesterol. Trial registration ClinicalTrials.gov, NCT01343589 PMID:23842081

  11. Decomposing Composing Conventions.

    ERIC Educational Resources Information Center

    Beers, Terry

    Recent research has invited critiques of the authoritative descriptions of composing found in many rhetoric textbooks. The concept of "convention" may be especially useful in rethinking the teleological basis of these textbook descriptions. Conventions found in composition textbooks need to be unmasked as arbitrary concepts which serve to…

  12. Quantifying the Displacement of Mismatches in Multiple Sequence Alignment Benchmarks

    PubMed Central

    Bawono, Punto; van der Velde, Arjan; Abeln, Sanne; Heringa, Jaap

    2015-01-01

    Multiple Sequence Alignment (MSA) methods are typically benchmarked on sets of reference alignments. The quality of the alignment can then be represented by the sum-of-pairs (SP) or column (CS) scores, which measure the agreement between a reference and corresponding query alignment. Both the SP and CS scores treat mismatches between a query and reference alignment as equally bad, and do not take the separation into account between two amino acids in the query alignment, that should have been matched according to the reference alignment. This is significant since the magnitude of alignment shifts is often of relevance in biological analyses, including homology modeling and MSA refinement/manual alignment editing. In this study we develop a new alignment benchmark scoring scheme, SPdist, that takes the degree of discordance of mismatches into account by measuring the sequence distance between mismatched residue pairs in the query alignment. Using this new score along with the standard SP score, we investigate the discriminatory behavior of the new score by assessing how well six different MSA methods perform with respect to BAliBASE reference alignments. The SP score and the SPdist score yield very similar outcomes when the reference and query alignments are close. However, for more divergent reference alignments the SPdist score is able to distinguish between methods that keep alignments approximately close to the reference and those exhibiting larger shifts. We observed that by using SPdist together with SP scoring we were able to better delineate the alignment quality difference between alternative MSA methods. With a case study we exemplify why it is important, from a biological perspective, to consider the separation of mismatches. The SPdist scoring scheme has been implemented in the VerAlign web server (http://www.ibi.vu.nl/programs/veralignwww/). The code for calculating SPdist score is also available upon request. PMID:25993129

  13. Nonlinear dynamics of inhomogeneous mismatched charged particle beams

    SciTech Connect

    Nunes, R. P.; Rizzato, F. B.

    2012-08-13

    This work analyzes the transversal dynamics of an inhomogeneous and mismatched charged particle beam. The beam is azimuthally symmetric, initially cold, and evolves in a linear channel permeated by an external constant magnetic field. Based on a Lagrangian approach, a low-dimensional model for the description of the beam dynamics has been obtained. The small set of nonlinear dynamical equations provided results that are in reasonable agreement with that ones observed in full self-consistent N-particle beam numerical simulations.

  14. Automatic prediction regarding the next state of a visual object: Electrophysiological indicators of prediction match and mismatch.

    PubMed

    Kimura, Motohiro; Takeda, Yuji

    2015-11-11

    Behavioral phenomena such as representational momentum suggest that the brain can automatically predict the next state of a visual object, based on sequential rules embedded in its preceding spatiotemporal context. To identify electrophysiological indicators of automatic visual prediction in terms of prediction match and mismatch, we recorded event-related brain potentials (ERPs) while participants passively viewed three types of task-irrelevant sequences of a bar stimulus: (1) an oddball sequence, which contained a sequential rule defined by stimulus repetition, providing repetition-rule-conforming (standard) and -violating (deviant) stimuli; (2) a rotating-oddball sequence, which contained a sequential rule defined by stimulus change (i.e., rotation), providing change-rule-conforming (regular) and -violating (irregular) stimuli; and (3) a random sequence, which did not contain a sequential rule, providing a neutral (control) stimulus. This protocol allowed us to expect that (1) an ERP effect that reflects a prediction-mismatch process should be exclusively observed in both the deviant-minus-control and irregular-minus-control comparisons and (2) an ERP effect that reflects a prediction-match process should be exclusively observed in both the standard-minus-control and regular-minus-control comparisons. The results showed that the ERP effect that met the criterion for prediction mismatch was an occipito-temporal negative deflection at around 170-300ms (visual mismatch negativity), while the ERP effect that met the criterion for prediction match was a frontal/central negative deflection at around 150-270ms (probably, the reduction of P2). These two contrasting ERP effects support a hypothetical view that automatic visual prediction would involve both an increase in the neural response to prediction-incongruent (i.e., novel) events and a decrease in the neural response to prediction-congruent (i.e., redundant) events. This article is part of a Special Issue entitled

  15. Feature-specific transition from positive mismatch response to mismatch negativity in early infancy: mismatch responses to vowels and initial consonants.

    PubMed

    Cheng, Ying-Ying; Wu, Hsin-Chi; Tzeng, Yu-Lin; Yang, Ming-Tao; Zhao, Lu-Lu; Lee, Chia-Ying

    2015-05-01

    This study investigated how phonological saliency, deviance size, and maturation affect mismatch responses (MMRs) in early infancy. MMRs to Mandarin vowels and initial consonants were measured using a multi-deviant oddball paradigm in adults, newborns, and 6-month-olds. The vowel condition consisted of Mandarin syllable da as the standard, du as the large deviant and di as small deviant. As for initial consonant condition, we took syllable ba as standard, ga as large deviant, and ba as small deviant. While adults showed typical mismatch negativities (MMNs), newborns demonstrated broad positive MMRs (P-MMRs) to both initial consonants and vowels. For 6-month-olds, deviance size affected the polarity of MMRs to vowels. The large deviant du/da contrast elicited an adult-like MMN, while the small deviant di/da contrast elicited a P-MMR. Initial consonant changes elicited only P-MMRs, regardless of deviance size. In summary, MMRs to vowels switched from P-MMR at birth to MMN at 6 months. However, the polarity transition was not found for MMRs to initial consonants. The developmental trajectories of MMRs to vowels and initial consonants further support the phonological saliency hypothesis. PMID:25819712

  16. Chromosomal directionality of DNA mismatch repair in Escherichia coli

    PubMed Central

    Hasan, A. M. Mahedi; Leach, David R. F.

    2015-01-01

    Defects in DNA mismatch repair (MMR) result in elevated mutagenesis and in cancer predisposition. This disease burden arises because MMR is required to correct errors made in the copying of DNA. MMR is bidirectional at the level of DNA strand polarity as it operates equally well in the 5′ to 3′ and the 3′ to 5′ directions. However, the directionality of MMR with respect to the chromosome, which comprises parental DNA strands of opposite polarity, has been unknown. Here, we show that MMR in Escherichia coli is unidirectional with respect to the chromosome. Our data demonstrate that, following the recognition of a 3-bp insertion-deletion loop mismatch, the MMR machinery searches for the first hemimethylated GATC site located on its origin-distal side, toward the replication fork, and that resection then proceeds back toward the mismatch and away from the replication fork. This study provides support for a tight coupling between MMR and DNA replication. PMID:26170312

  17. Towards automatic identification of mismatched image pairs through loop constraints

    NASA Astrophysics Data System (ADS)

    Elibol, Armagan; Kim, Jinwhan; Gracias, Nuno; Garcia, Rafael

    2013-12-01

    Obtaining image sequences has become easier and easier thanks to the rapid progress on optical sensors and robotic platforms. Processing of image sequences (e.g., mapping, 3D reconstruction, Simultaneous Localisation and Mapping (SLAM)) usually requires 2D image registration. Recently, image registration is accomplished by detecting salient points in two images and nextmatching their descriptors. To eliminate outliers and to compute a planar transformation (homography) between the coordinate frames of images, robust methods (such as Random Sample Consensus (RANSAC) and Least Median of Squares (LMedS)) are employed. However, image registration pipeline can sometimes provide sufficient number of inliers within the error bounds even when images do not overlap. Such mismatches occur especially when the scene has repetitive texture and shows structural similarity. In this study, we present a method to identify the mismatches using closed-loop (cycle) constraints. The method exploits the fact that images forming a cycle should have identity mapping when all the homographies between images in the cycle multiplied. Cycles appear when the camera revisits an area that was imaged before, which is a common practice especially for mapping purposes. Our proposal extracts several cycles to obtain error statistics for each matched image pair. Then, it searches for image pairs that have extreme error histogram comparing to the other pairs. We present experimental results with artificially added mismatched image pairs on real underwater image sequences.

  18. Review: Clinical aspects of hereditary DNA Mismatch repair gene mutations.

    PubMed

    Sijmons, Rolf H; Hofstra, Robert M W

    2016-02-01

    Inherited mutations of the DNA Mismatch repair genes MLH1, MSH2, MSH6 and PMS2 can result in two hereditary tumor syndromes: the adult-onset autosomal dominant Lynch syndrome, previously referred to as Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the childhood-onset autosomal recessive Constitutional Mismatch Repair Deficiency syndrome. Both conditions are important to recognize clinically as their identification has direct consequences for clinical management and allows targeted preventive actions in mutation carriers. Lynch syndrome is one of the more common adult-onset hereditary tumor syndromes, with thousands of patients reported to date. Its tumor spectrum is well established and includes colorectal cancer, endometrial cancer and a range of other cancer types. However, surveillance for cancers other than colorectal cancer is still of uncertain value. Prophylactic surgery, especially for the uterus and its adnexa is an option in female mutation carriers. Chemoprevention of colorectal cancer with aspirin is actively being investigated in this syndrome and shows promising results. In contrast, the Constitutional Mismatch Repair Deficiency syndrome is rare, features a wide spectrum of childhood onset cancers, many of which are brain tumors with high mortality rates. Future studies are very much needed to improve the care for patients with this severe disorder. PMID:26746812

  19. JPEG quantization table mismatched steganalysis via robust discriminative feature transformation

    NASA Astrophysics Data System (ADS)

    Zeng, Likai; Kong, Xiangwei; Li, Ming; Guo, Yanqing

    2015-03-01

    The cover source mismatch is a common problem in steganalysis, which may result in the degradation of detection accuracy. In this paper, we present a novel method to mitigate the problem of JPEG quantization table mismatch, named as Robust Discriminative Feature Transformation (RDFT). RDFT transforms original features to new feature representations based on a non-linear transformation matrix. It can improve the statistical consistency of the training samples and testing samples and learn new matched feature representations from original features by minimizing feature distribution difference while preserving the classification ability of training data. The comparison to prior arts reveals that the detection accuracy of the proposed RDFT algorithm can significantly outperform traditional steganalyzers under mismatched conditions and it is close to that of matched scenario. RDFT has several appealing advantages: 1) it can improve the statistical consistency of the training and testing data; 2) it can reduce the distribution difference between the training features and testing features; 3) it can preserve the classification ability of the training data; 4) it is robust to parameters and can achieve a good performance under a wide range of parameter values.

  20. Cation-size-mismatch tuning of photoluminescence in oxynitride phosphors.

    PubMed

    Chen, Wei-Ting; Sheu, Hwo-Shuenn; Liu, Ru-Shi; Attfield, J Paul

    2012-05-16

    Red or yellow phosphors excited by a blue light-emitting diode are an efficient source of white light for everyday applications. Many solid oxides and nitrides, particularly silicon nitride-based materials such as M(2)Si(5)N(8) and MSi(2)O(2)N(2) (M = Ca, Sr, Ba), CaAlSiN(3), and SiAlON, are useful phosphor hosts with good thermal stabilities. Both oxide/nitride and various cation substitutions are commonly used to shift the emission spectrum and optimize luminescent properties, but the underlying mechanisms are not always clear. Here we show that size-mismatch between host and dopant cations tunes photoluminescence shifts systematically in M(1.95)Eu(0.05)Si(5-x)Al(x)N(8-x)O(x) lattices, leading to a red shift when the M = Ba and Sr host cations are larger than the Eu(2+) dopant, but a blue shift when the M = Ca host is smaller. Size-mismatch tuning of thermal quenching is also observed. A local anion clustering mechanism in which Eu(2+) gains excess nitride coordination in the M = Ba and Sr structures, but excess oxide in the Ca analogues, is proposed for these mismatch effects. This mechanism is predicted to be general to oxynitride materials and will be useful in tuning optical and other properties that are sensitive to local coordination environments. PMID:22534019

  1. Investigating interaural frequency-place mismatches via bimodal vowel integration.

    PubMed

    Guérit, François; Santurette, Sébastien; Chalupper, Josef; Dau, Torsten

    2014-01-01

    For patients having residual hearing in one ear and a cochlear implant (CI) in the opposite ear, interaural place-pitch mismatches might be partly responsible for the large variability in individual benefit. Behavioral pitch-matching between the two ears has been suggested as a way to individualize the fitting of the frequency-to-electrode map but is rather tedious and unreliable. Here, an alternative method using two-formant vowels was developed and tested. The interaural spectral shift was inferred by comparing vowel spaces, measured by presenting the first formant (F1) to the nonimplanted ear and the second (F2) on either side. The method was first evaluated with eight normal-hearing listeners and vocoder simulations, before being tested with 11 CI users. Average vowel distributions across subjects showed a similar pattern when presenting F2 on either side, suggesting acclimatization to the frequency map. However, individual vowel spaces with F2 presented to the implant did not allow a reliable estimation of the interaural mismatch. These results suggest that interaural frequency-place mismatches can be derived from such vowel spaces. However, the method remains limited by difficulties in bimodal fusion of the two formants. PMID:25421087

  2. Semiblind Hyperspectral Unmixing in the Presence of Spectral Library Mismatches

    NASA Astrophysics Data System (ADS)

    Fu, Xiao; Ma, Wing-Kin; Bioucas-Dias, Jose M.; Chan, Tsung-Han

    2016-09-01

    The dictionary-aided sparse regression (SR) approach has recently emerged as a promising alternative to hyperspectral unmixing (HU) in remote sensing. By using an available spectral library as a dictionary, the SR approach identifies the underlying materials in a given hyperspectral image by selecting a small subset of spectral samples in the dictionary to represent the whole image. A drawback with the current SR developments is that an actual spectral signature in the scene is often assumed to have zero mismatch with its corresponding dictionary sample, and such an assumption is considered too ideal in practice. In this paper, we tackle the spectral signature mismatch problem by proposing a dictionary-adjusted nonconvex sparsity-encouraging regression (DANSER) framework. The main idea is to incorporate dictionary correcting variables in an SR formulation. A simple and low per-iteration complexity algorithm is tailor-designed for practical realization of DANSER. Using the same dictionary correcting idea, we also propose a robust subspace solution for dictionary pruning. Extensive simulations and real-data experiments show that the proposed method is effective in mitigating the undesirable spectral signature mismatch effects.

  3. Halo formation from mismatched beam-beam interactions

    SciTech Connect

    Qiang, Ji

    2003-05-23

    In this paper, we report on the halo formation and emittance growth driven by a parametric resonance during mismatched beam-beam collisions. In the regime of the weak-strong beam-beam interaction, if two beams have the same machine tunes, on-axis head-on collisions between a mismatched strong beam and a weak beam will not cause the formation of halo. However, if the two beams collide with an initial offset, the beam-beam force from the mismatched strong beam can cause halo formation and emittance growth in the weak beam. Meanwhile, if two beams have different machine tunes, for opposite charged colliding beams, when the machine tune of the weak beam is smaller than that of strong beam, there is emittance growth in the weak beam. When the machine tune of the weak beam is larger than that of the strong beam, there is little emittance growth. In the regime of strong-strong beam-beam interaction, halo is formed in both beams even when the two beams collide head-on on the axis with equal machine tunes. This puts a strong requirement for a good beam match during the injection to colliders in order to avoid the emittance growth.

  4. Infra-red parametric generation: Phase mismatch condition

    SciTech Connect

    Ghosh, S.; Dubey, Swati; Jain, Kamal

    2015-07-31

    An analytical investigation is made for the Infrared parametric generation in doped semiconductor plasma under phase mismatch condition. Theoretical formulations are undertaken to determine induced polarization and threshold pump field for the onset of parametric generation in semiconductor plasma medium. The origin of this nonlinear interaction lies in the second order optical susceptibility arising due to the induced nonlinear current density in piezoelectric medium. Numerical estimations are made for n- type InSb at 77 K duly irradiated by a pulsed 10.6µm CO{sub 2} laser. It is very difficult to attain exact phase matching in experimental frame so we have considered a tolerable small phase mismatch in order to attain a new result. Its effect on the Infrared parametric generation in compound semiconductor is examined through induced polarization. Transmitted intensity is determined to have an idea about conversion efficiency of the said process. Phase mismatch tends to raise the required pump field to stimulate the parametric generation. Transmitted intensity is found to decrease with coherence length lc and increase carrier concentration n{sub 0}, which is favorable for improved conversion efficiency.

  5. Investigating Interaural Frequency-Place Mismatches via Bimodal Vowel Integration

    PubMed Central

    Santurette, Sébastien; Chalupper, Josef; Dau, Torsten

    2014-01-01

    For patients having residual hearing in one ear and a cochlear implant (CI) in the opposite ear, interaural place-pitch mismatches might be partly responsible for the large variability in individual benefit. Behavioral pitch-matching between the two ears has been suggested as a way to individualize the fitting of the frequency-to-electrode map but is rather tedious and unreliable. Here, an alternative method using two-formant vowels was developed and tested. The interaural spectral shift was inferred by comparing vowel spaces, measured by presenting the first formant (F1) to the nonimplanted ear and the second (F2) on either side. The method was first evaluated with eight normal-hearing listeners and vocoder simulations, before being tested with 11 CI users. Average vowel distributions across subjects showed a similar pattern when presenting F2 on either side, suggesting acclimatization to the frequency map. However, individual vowel spaces with F2 presented to the implant did not allow a reliable estimation of the interaural mismatch. These results suggest that interaural frequency-place mismatches can be derived from such vowel spaces. However, the method remains limited by difficulties in bimodal fusion of the two formants. PMID:25421087

  6. Method for reducing or eliminating interface defects in mismatched semiconductor epilayers

    DOEpatents

    Fitzgerald, E.A. Jr.; Ast, D.G.

    1992-10-20

    The present invention and process relates to crystal lattice mismatched semiconductor composite having a first semiconductor layer and a second semiconductor growth layer deposited thereon to form an interface wherein the growth layer can be deposited at thicknesses in excess of the critical thickness, even up to about 10[times] critical thickness. Such composite has an interface which is substantially free of interface defects. For example, the size of the growth areas in a mismatched In[sub 0.05]Ga[sub 0.95]As/(001)GaAs interface was controlled by fabricating 2-[mu]m high pillars of various lateral geometries and lateral dimensions before the epitaxial deposition of 3500 [angstrom] of In[sub 0.05]Ga[sub 0.95]As. The linear dislocation density at the interface was reduced from >5000 dislocations/cm to about zero for 25-[mu]m lateral dimensions and to less than 800 dislocations/cm for lateral dimensions as large as 100 [mu]m. The fabricated pillars control the lateral dimensions of the growth layer and block the glide of misfit dislocations with the resultant decrease in dislocation density. 7 figs.

  7. Method for reducing or eliminating interface defects in mismatched semiconductor epilayers

    DOEpatents

    Fitzgerald, Jr., Eugene A.; Ast, Dieter G.

    1992-01-01

    The present invention and process relates to crystal lattice mismatched semiconductor composite having a first semiconductor layer and a second semiconductor growth layer deposited thereon to form an interface wherein the growth layer can be deposited at thicknesses in excess of the critical thickness, even up to about 10.times. critical thickness. Such composite has an interface which is substantially free of interface defects. For example, the size of the growth areas in a mismatched In.sub.0.05 Ga.sub.0.95 As/(001)GaAs interface was controlled by fabricating 2-.mu.m high pillars of various lateral geometries and lateral dimensions before the epitaxial deposition of 3500.ANG. of In.sub.0.05 Ga.sub.0.95 As. The linear dislocation density at the interface was reduced from >5000 dislocations/cm to about zero for 25-.mu.m lateral dimensions and to less than 800 dislocations/cm for lateral dimensions as large as 100 .mu.m. The fabricated pillars control the lateral dimensions of the growth layer and block the glide of misfit dislocations with the resultant decrease in dislocation density.

  8. Method for reducing or eliminating interface defects in mismatched semiconductor eiplayers

    DOEpatents

    Fitzgerald, Jr., Eugene A.; Ast, Dieter G.

    1991-01-01

    The present invention and process relates to crystal lattice mismatched semiconductor composite having a first semiconductor layer and a second semiconductor growth layer deposited thereon to form an interface wherein the growth layer can be deposited at thicknesses in excess of the critical thickness, even up to about 10x critical thickness. Such composite has an interface which is substantially free of interface defects. For example, the size of the growth areas in a mismatched In.sub.0.05 Ga.sub.0.95 As/(001)GaAs interface was controlled by fabricating 2-.mu.m high pillars of various lateral geometries and lateral dimensions before the epitaxial deposition of 3500.ANG. of In.sub.0.05 Ga.sub.0.95 As. The linear dislocation density at the interface was reduced from >5000 dislocations/cm to about zero for 25-.mu.m lateral dimensions and to less than 800 dislocations/cm for lateral dimensions as large as 100 .mu.m. The fabricated pillars control the lateral dimensions of the growth layer and block the glide of misfit dislocations with the resultant decrease in dislocation density.

  9. Insertion and Deletion Mismatches Distant from the Target Position Improve Gene Correction with a Tailed Duplex.

    PubMed

    Kamiya, Hiroyuki; Nishigaki, Natsuki; Ikeda, Akihiro; Yukawa, Seiya; Morita, Yukiko; Nakatsu, Yoshimichi; Tsuzuki, Teruhisa; Harashima, Hideyoshi

    2016-07-01

    A 5'-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches ∼330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment. PMID:27253876

  10. Tokamak coordinate conventions: COCOS

    NASA Astrophysics Data System (ADS)

    Sauter, O.; Medvedev, S. Yu.

    2013-02-01

    Dealing with electromagnetic fields, in particular current and related magnetic fields, yields "natural" physical vector relations in 3-D. However, when it comes to choosing local coordinate systems, the "usual" right-handed systems are not necessarily the best choices, which means that there are several options being chosen. In the magnetic fusion community such a difficulty exists for the choices of the cylindrical and of the toroidal coordinate systems. In addition many codes depend on knowledge of an equilibrium. In particular, the Grad-Shafranov axisymmetric equilibrium solution for tokamak plasmas, ψ, does not depend on the sign of the plasma current Ip nor that of the magnetic field B0. This often results in ill-defined conventions. Moreover the sign, amplitude and offset of ψ are of less importance, since the free sources in the equation depend on the normalized radial coordinate. The signs of the free sources, dp/dψ and dF2/dψ (p being the pressure, ψ the poloidal magnetic flux and F=RBφ), must be consistent to generate the current density profile. For example, RF and CD calculations (Radio Frequency heating and Current Drive) require an exact sign convention in order to calculate a co- or counter-CD component. It is shown that there are over 16 different coordinate conventions. This paper proposes a unique identifier, the COCOS convention, to distinguish between the 16 most-commonly used options. Given the present worldwide efforts towards code integration, the proposed new index COCOS defining uniquely the COordinate COnventionS required as input by a given code or module is particularly useful. As codes use different conventions, it is useful to allow different sign conventions for equilibrium code input and output, equilibrium being at the core of any calculations in magnetic fusion. Additionally, given two different COCOS conventions, it becomes simple to transform between them. The relevant transformations are described in detail.

  11. A Quiet Convention.

    ERIC Educational Resources Information Center

    Suggs, Welch

    2003-01-01

    Describes how discussion of governance and academic standards dominated the proceedings at the first NCAA convention of Myles Brand's presidency. The new president also offered a qualified endorsement of Title IX. (EV)

  12. Cincinnati; Our Convention City

    ERIC Educational Resources Information Center

    Borchin, Anna

    1970-01-01

    During Easter week, 1971, Cincinnati will be the hostess of the 50th anniversary convention of the Catholic Library Association. Items of historical interest concerning the city are briefly described. (NH)

  13. Mandibular changes during initial alignment with SmartClip self-ligating and conventional brackets: A single-center prospective randomized controlled clinical trial

    PubMed Central

    Bayram, Mehmet; Nur, Metin; Kilkis, Dogan

    2015-01-01

    Objective To test the null hypothesis that SmartClip self-ligating brackets are more effective than conventional brackets for initial mandibular alignment and identify influential factors. Methods Fifty patients were randomly allocated to two equal treatment groups by using an online randomization program: self-ligating group (SmartClip brackets) and conventional group (Gemini brackets). The archwire sequence was standardized. Changes in anterior irregularity index, intercanine width, and intermolar width were assessed on plaster models at 8th and 16th weeks. Changes in incisor position and inclination were assessed on lateral cephalometric radiographs at 16 weeks. Intragroup and intergroup comparisons were performed with paired t-test and Student's t-test, respectively. Multiple linear regression was performed to identify variables affecting improvement in anterior ambiguity. Results Data of 46 patients were analyzed; those missing an appointment (n = 2) or showing bracket breakage (n = 2) were excluded. Incisor inclination (p < 0.05), intercanine width (p < 0.05), and intermolar width (p > 0.05) increased at 8 and 16 weeks in both the groups; no significant intergroup differences were noted (p > 0.05). Initial anterior irregularity index and intercanine width change were significantly associated with improvement in anterior irregularity (p < 0.001). Conclusions The null hypothesis was rejected. Bracket type has little effect on improvement in anterior ambiguity during initial mandibular alignment. PMID:25798415

  14. Comparison of Tubal Sterilization Procedures Performed by Keyless Abdominal Rope-Lifting Surgery and Conventional CO2 Laparoscopy: A Case Controlled Clinical Study

    PubMed Central

    Hüseyinoğlu, Ürfettin

    2013-01-01

    Objective. To evaluate the safety and efficacy of Keyless Abdominal Rope-Lifting Surgery (KARS), for tubal sterilization procedures in comparison with the conventional CO2 laparoscopy. Material and Methods. During a one-year period, 71 women underwent tubal ligation surgery. Conventional laparoscopy (N = 38) and KARS (N = 33) were used for tubal sterilization. In KARS, an abdominal access pathway through a single intra-abdominal incision was used to place transabdominal sutures that elevated the abdominal wall, and the operations were performed through the intraumbilical entry without the use of trocars. In CO2 laparoscopy, following the creation of the CO2 pneumoperitoneum a 10 mm trocar and two 5 mm trocars were introduced into the abdominal cavity. Tubal sterilizations were performed following the creation of the abdominal access pathways in both groups. The groups were compared with each other. Results. All operations could be performed by KARS without conversion to CO2 laparoscopy or laparotomy. The mean operative time of the two groups was not significantly different (P > 0.05). Intra- and postoperative findings including complications, bleeding, and hospital stay time did not differ between groups (P > 0.05). Conclusion. KARS for tubal sterilization seems safe and effective in terms of cosmesis, postoperative pain, and early hospital discharge. PMID:24453932

  15. Cryoplasty Versus Conventional Balloon Angioplasty of the Femoropopliteal Artery in Diabetic Patients: Long-Term Results from a Prospective Randomized Single-Center Controlled Trial

    SciTech Connect

    Spiliopoulos, Stavros Katsanos, Konstantinos; Karnabatidis, Dimitris; Diamantopoulos, Athanasios; Kagadis, George C.; Christeas, Nikolaos; Siablis, Dimitris

    2010-10-15

    The purpose of this study was to investigate the immediate and long-term results of cryoplasty versus conventional balloon angioplasty in the femoropopliteal artery of diabetic patients. Fifty diabetic patients (41 men, mean age 68 years) were randomized to cryoplasty (group CRYO; 24 patients with 31 lesions) or conventional balloon angioplasty (group COBA; 26 patients with 34 lesions) of the femoropopliteal artery. Technical success was defined as <30% residual stenosis without any adjunctive stenting. Primary end points included technical success, primary patency, binary in-lesion restenosis (>50%), and freedom from target lesion recanalization. Cox proportional hazards regression analysis was performed to adjust for confounding factors of heterogeneity. In total, 61.3% (19 of 31) in group CRYO and 52.9% (18 of 34) in group COBA were de novo lesions. More than 70% of the lesions were Transatlantic Inter-Society Consensus (TASC) B and C in both groups, and 41.4% of the patients in group CRYO and 38.7% in group COBA suffered from critical limb ischemia. Immediate technical success rate was 58.0% in group CRYO versus 64.0% in group COBA (p = 0.29). According to 3-year Kaplan-Meier estimates, there were no significant differences with regard to patient survival (86.8% in group CRYO vs. 87.0% in group COBA, p = 0.54) and limb salvage (95.8 vs. 92.1% in groups CRYO and COBA, respectively, p = 0.60). There was a nonsignificant trend of increased binary restenosis in group CRYO (hazard ratio [HR] 1.3; 95% CI 0.6-2.6, p = 0.45). Primary patency was significantly lower in group CRYO compared with group COBA (HR 2.2; 95% CI 1.1-4.3, p = 0.02). Significantly more repeat intervention events because of recurrent symptoms were required in group CRYO (HR 2.5; 95% CI 1.2-5.3, p = 0.01). Cryoplasty was associated with lower primary patency and more clinically driven repeat procedures after long-term follow-up compared with conventional balloon angioplasty.

  16. Digital Full-Scope Simulation of a Conventional Nuclear Power Plant Control Room, Phase 2: Installation of a Reconfigurable Simulator to Support Nuclear Plant Sustainability

    SciTech Connect

    Ronald L. Boring; Vivek Agarwal; Kirk Fitzgerald; Jacques Hugo; Bruce Hallbert

    2013-03-01

    The U.S. Department of Energy’s Light Water Reactor Sustainability program has developed a control room simulator in support of control room modernization at nuclear power plants in the U.S. This report highlights the recent completion of this reconfigurable, full-scale, full-scope control room simulator buildout at the Idaho National Laboratory. The simulator is fully reconfigurable, meaning it supports multiple plant models developed by different simulator vendors. The simulator is full-scale, using glasstop virtual panels to display the analog control boards found at current plants. The present installation features 15 glasstop panels, uniquely achieving a complete control room representation. The simulator is also full-scope, meaning it uses the same plant models used for training simulators at actual plants. Unlike in the plant training simulators, the deployment on glasstop panels allows a high degree of customization of the panels, allowing the simulator to be used for research on the design of new digital control systems for control room modernization. This report includes separate sections discussing the glasstop panels, their layout to mimic control rooms at actual plants, technical details on creating a multi-plant and multi-vendor reconfigurable simulator, and current efforts to support control room modernization at U.S. utilities. The glasstop simulator provides an ideal testbed for prototyping and validating new control room concepts. Equally importantly, it is helping create a standardized and vetted human factors engineering process that can be used across the nuclear industry to ensure control room upgrades maintain and even improve current reliability and safety.

  17. Design and numerical evaluation of full-authority flight control systems for conventional and thruster-augmented helicopters employed in NOE operations

    NASA Technical Reports Server (NTRS)

    Perri, Todd A.; Mckillip, R. M., Jr.; Curtiss, H. C., Jr.

    1987-01-01

    The development and methodology is presented for development of full-authority implicit model-following and explicit model-following optimal controllers for use on helicopters operating in the Nap-of-the Earth (NOE) environment. Pole placement, input-output frequency response, and step input response were used to evaluate handling qualities performance. The pilot was equipped with velocity-command inputs. A mathematical/computational trajectory optimization method was employed to evaluate the ability of each controller to fly NOE maneuvers. The method determines the optimal swashplate and thruster input histories from the helicopter's dynamics and the prescribed geometry and desired flying qualities of the maneuver. Three maneuvers were investigated for both the implicit and explicit controllers with and without auxiliary propulsion installed: pop-up/dash/descent, bob-up at 40 knots, and glideslope. The explicit controller proved to be superior to the implicit controller in performance and ease of design.

  18. Effect of HLA-Matching Recipients to Donor Noninherited Maternal Antigens on Outcomes after Mismatched Umbilical Cord Blood Transplantation for Hematologic Malignancy

    PubMed Central

    Rocha, Vanderson; Spellman, Stephen; Zhang, Mei-Jie; Ruggeri, Annalisa; Purtill, Duncan; Brady, Colleen; Baxter-Lowe, Lee Ann; Baudoux, Etienne; Bergamaschi, Paola; Chow, Robert; Freed, Brian; Koegler, Gesine; Kurtzberg, Joanne; Larghero, Jerome; Lecchi, Lucilla; Nagler, Arnon; Navarrette, Cristina; Prasad, Vinod; Pouthier, Fabienne; Price, Thomas; Ratanatharathorn, Voravit; van Rood, Jon J.; Horowitz, Mary M.; Gluckman, Eliane; Eapen, Mary

    2013-01-01

    Transplantation-related mortality (TRM) is high after HLA-mismatched umbilical cord blood (UCB) transplantation (UCBT). In utero, exposure to noninherited maternal antigen (NIMA) is recognized by the fetus, which induces Tregulator cells to that haplotype. It is plausible that UCBTs in which recipients are matched to donor NIMAs may alleviate some of the excess mortality associated with this treatment. To explore this concept, we used marginal matched-pair Cox regression analysis to compare outcomes in 48 NIMA-matched UCBTs (ie, the NIMA of the donor UCB unit matched to the patient) and in 116 non–NIMA-matched UCBTs. All patients had a hematologic malignancy and received a single UCB unit. Cases and controls were matched on age, disease, disease status, transplantation-conditioning regimen, HLA match, and infused cell dose. TRM was lower after NIMA-matched UCBTs compared with NIMA-mismatched UCBTs (relative risk, 0.48; P=.05; 18% versus 32% at 5 years posttransplantation). Consequently, overall survival was higher after NIMA-matched UCBT. The 5-year probability of overall survival was 55% after NIMA-matched UCBTs versus 38% after NIMA-mismatched UCBTs (P=.04). When faced with the choice of multiple HLA-mismatched UCB units containing adequate cell doses, selecting an NIMA-matched UCB unit may improve survival after mismatched UCBT. PMID:22814031

  19. Effect of HLA-matching recipients to donor noninherited maternal antigens on outcomes after mismatched umbilical cord blood transplantation for hematologic malignancy.

    PubMed

    Rocha, Vanderson; Spellman, Stephen; Zhang, Mei-Jie; Ruggeri, Annalisa; Purtill, Duncan; Brady, Colleen; Baxter-Lowe, Lee Ann; Baudoux, Etienne; Bergamaschi, Paola; Chow, Robert; Freed, Brian; Koegler, Gesine; Kurtzberg, Joanne; Larghero, Jerome; Lecchi, Lucilla; Nagler, Arnon; Navarrette, Cristina; Prasad, Vinod; Pouthier, Fabienne; Price, Thomas; Ratanatharathorn, Voravit; van Rood, Jon J; Horowitz, Mary M; Gluckman, Eliane; Eapen, Mary

    2012-12-01

    Transplantation-related mortality (TRM) is high after HLA-mismatched umbilical cord blood (UCB) transplantation (UCBT). In utero, exposure to noninherited maternal antigen (NIMA) is recognized by the fetus, which induces T regulator cells to that haplotype. It is plausible that UCBTs in which recipients are matched to donor NIMAs may alleviate some of the excess mortality associated with this treatment. To explore this concept, we used marginal matched-pair Cox regression analysis to compare outcomes in 48 NIMA-matched UCBTs (ie, the NIMA of the donor UCB unit matched to the patient) and in 116 non-NIMA-matched UCBTs. All patients had a hematologic malignancy and received a single UCB unit. Cases and controls were matched on age, disease, disease status, transplantation-conditioning regimen, HLA match, and infused cell dose. TRM was lower after NIMA-matched UCBTs compared with NIMA-mismatched UCBTs (relative risk, 0.48; P = .05; 18% versus 32% at 5 years posttransplantation). Consequently, overall survival was higher after NIMA-matched UCBT. The 5-year probability of overall survival was 55% after NIMA-matched UCBTs versus 38% after NIMA-mismatched UCBTs (P = .04). When faced with the choice of multiple HLA-mismatched UCB units containing adequate cell doses, selecting an NIMA-matched UCB unit may improve survival after mismatched UCBT. PMID:22814031

  20. Validation of doubled haploid plants by enzymatic mismatch cleavage

    PubMed Central

    2013-01-01

    Background Doubled haploidy is a fundamental tool in plant breeding as it provides the fastest way to generate populations of meiotic recombinants in a genetically fixed state. A wide range of methods has been developed to produce doubled haploid (DH) plants and recent advances promise efficient DH production in otherwise recalcitrant species. Since the cellular origin of the plants produced is not always certain, rapid screening techniques are needed to validate that the produced individuals are indeed homozygous and genetically distinct from each other. Ideal methods are easily implemented across species and in crops where whole genome sequence and marker resources are limited. Results We have adapted enzymatic mismatch cleavage techniques commonly used for TILLING (Targeting Induced Local Lesions IN Genomes) for the evaluation of heterozygosity in parental, F1 and putative DH plants. We used barley as a model crop and tested 26 amplicons previously developed for TILLING. Experiments were performed using self-extracted single-strand-specific nuclease and standard native agarose gels. Eleven of the twenty-six tested primers allowed unambiguous assignment of heterozygosity in material from F1 crosses and loss of heterozygosity in the DH plants. Through parallel testing of previously developed Simple Sequence Repeat (SSR) markers, we show that 3/32 SSR markers were suitable for screening. This suggests that enzymatic mismatch cleavage approaches can be more efficient than SSR based screening, even in species with well-developed markers. Conclusions Enzymatic mismatch cleavage has been applied for mutation discovery in many plant species, including those with little or no available genomic DNA sequence information. Here, we show that the same methods provide an efficient system to screen for the production of DH material without the need of specialized equipment. This gene target based approach further allows discovery of novel nucleotide polymorphisms in candidate

  1. Triple helix structures: sequence dependence, flexibility and mismatch effects.

    PubMed

    Sun, J S; Mergny, J L; Lavery, R; Montenay-Garestier, T; Hélène, C

    1991-12-01

    By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine. homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen-bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption. PMID:1815635

  2. Shells on lattice-mismatched colloidal spheres, cubes, and peanuts

    NASA Astrophysics Data System (ADS)

    Sindoro, Melinda; Granick, Steve

    2015-03-01

    Cavities form spontaneously due to geometrical frustration when crystalline shells is gradually grown on non-linear surfaces. This we conclude experimentally from growing lattice mismatched shells on colloidal spheres, cubes, and peanuts, all of them providing different local curvature. According to the core shape, the underlying interfacial curvature promotes different cavity formation which we can follow over time. The resulting spatio-temporal heterogeneity adds up to a propagation of an increasingly strong mechanical stress at the core-shell interface, inducing core-shells transformation to yolk-shells.

  3. Halo formation in mismatched, space-charge-dominated beams

    SciTech Connect

    Bohn, C.L.; Delayen, J.R.

    1993-08-01

    A semianalytic formalism was recently developed for investigating the transverse dynamics of a mismatched, space-charge-dominated beam propagating through a focusing channel. It uses the Fokker-Planck equation to account for the rapid evolution of the coarse-grained distribution function in the phase space of a single beam particle. A simple model of dynamical friction and diffusion represents the effects of turbulence resulting from charge redistribution. The initial application was to sheet beams. In this paper, the formalism is generalized to fully two-dimensional beams.

  4. HLA-C expression levels define permissible mismatches in hematopoietic cell transplantation.

    PubMed

    Petersdorf, Effie W; Gooley, Theodore A; Malkki, Mari; Bacigalupo, Andrea P; Cesbron, Anne; Du Toit, Ernette; Ehninger, Gerhard; Egeland, Torstein; Fischer, Gottfried F; Gervais, Thibaut; Haagenson, Michael D; Horowitz, Mary M; Hsu, Katharine; Jindra, Pavel; Madrigal, Alejandro; Oudshoorn, Machteld; Ringdén, Olle; Schroeder, Marlis L; Spellman, Stephen R; Tiercy, Jean-Marie; Velardi, Andrea; Witt, Campbell S; O'Huigin, Colm; Apps, Richard; Carrington, Mary

    2014-12-18

    Life-threatening graft-versus-host disease (GVHD) limits the use of HLA-C-mismatched unrelated donors in transplantation. Clinicians lack criteria for donor selection when HLA-C-mismatched donors are a patient's only option for cure. We examined the role for HLA-C expression levels to identify permissible HLA-C mismatches. The median fluorescence intensity, a proxy of HLA-C expression, was assigned to each HLA-C allotype in 1975 patients and their HLA-C-mismatched unrelated transplant donors. The association of outcome with the level of expression of patients' and donors' HLA-C allotypes was evaluated in multivariable models. Increasing expression level of the patient's mismatched HLA-C allotype was associated with increased risks of grades III to IV acute GVHD, nonrelapse mortality, and mortality. Increasing expression level among HLA-C mismatches with residue 116 or residue 77/80 mismatching was associated with increased nonrelapse mortality. The immunogenicity of HLA-C mismatches in unrelated donor transplantation is influenced by the expression level of the patient's mismatched HLA-C allotype. HLA-C expression levels provide new information on mismatches that should be avoided and extend understanding of HLA-C-mediated immune responses in human disease. PMID:25323824

  5. HLA-C expression levels define permissible mismatches in hematopoietic cell transplantation

    PubMed Central

    Gooley, Theodore A.; Malkki, Mari; Bacigalupo, Andrea P.; Cesbron, Anne; Du Toit, Ernette; Ehninger, Gerhard; Egeland, Torstein; Fischer, Gottfried F.; Gervais, Thibaut; Haagenson, Michael D.; Horowitz, Mary M.; Hsu, Katharine; Jindra, Pavel; Madrigal, Alejandro; Oudshoorn, Machteld; Ringdén, Olle; Schroeder, Marlis L.; Spellman, Stephen R.; Tiercy, Jean-Marie; Velardi, Andrea; Witt, Campbell S.; O’Huigin, Colm; Apps, Richard; Carrington, Mary

    2014-01-01

    Life-threatening graft-versus-host disease (GVHD) limits the use of HLA-C-mismatched unrelated donors in transplantation. Clinicians lack criteria for donor selection when HLA-C-mismatched donors are a patient’s only option for cure. We examined the role for HLA-C expression levels to identify permissible HLA-C mismatches. The median fluorescence intensity, a proxy of HLA-C expression, was assigned to each HLA-C allotype in 1975 patients and their HLA-C-mismatched unrelated transplant donors. The association of outcome with the level of expression of patients’ and donors’ HLA-C allotypes was evaluated in multivariable models. Increasing expression level of the patient’s mismatched HLA-C allotype was associated with increased risks of grades III to IV acute GVHD, nonrelapse mortality, and mortality. Increasing expression level among HLA-C mismatches with residue 116 or residue 77/80 mismatching was associated with increased nonrelapse mortality. The immunogenicity of HLA-C mismatches in unrelated donor transplantation is influenced by the expression level of the patient’s mismatched HLA-C allotype. HLA-C expression levels provide new information on mismatches that should be avoided and extend understanding of HLA-C-mediated immune responses in human disease. PMID:25323824

  6. Mismatch in microbial food webs: predators but not prey perform better in their local biotic and abiotic conditions.

    PubMed

    Parain, Elodie C; Gravel, Dominique; Rohr, Rudolf P; Bersier, Louis-Félix; Gray, Sarah M

    2016-07-01

    Understanding how trophic levels respond to changes in abiotic and biotic conditions is key for predicting how food webs will react to environmental perturbations. Different trophic levels may respond disproportionately to change, with lower levels more likely to react faster, as they typically consist of smaller-bodied species with higher reproductive rates. This response could cause a mismatch between trophic levels, in which predators and prey will respond differently to changing abiotic or biotic conditions. This mismatch between trophic levels could result in altered top-down and bottom-up control and changes in interaction strength. To determine the possibility of a mismatch, we conducted a reciprocal-transplant experiment involving Sarracenia purpurea food webs consisting of bacterial communities as prey and a subset of six morphologically similar protozoans as predators. We used a factorial design with four temperatures, four bacteria and protozoan biogeographic origins, replicated four times. This design allowed us to determine how predator and prey dynamics were altered by abiotic (temperature) conditions and biotic (predators paired with prey from either their local or non-local biogeographic origin) conditions. We found that prey reached higher densities in warmer temperature regardless of their temperature of origin. Conversely, predators achieved higher densities in the temperature condition and with the prey from their origin. These results confirm that predators perform better in abiotic and biotic conditions of their origin while their prey do not. This mismatch between trophic levels may be especially significant under climate change, potentially disrupting ecosystem functioning by disproportionately affecting top-down and bottom-up control. PMID:27547320

  7. Lower Loading Dose of Prasugrel Compared with Conventional Loading Doses of Clopidogrel and Prasugrel in Korean Patients Undergoing Elective Coronary Angiography: A Randomized Controlled Study Evaluating Pharmacodynamic Efficacy

    PubMed Central

    Lee, Dong Hyun; Guo, Long Zhe; Park, Min Kyu; Yi, So Jeong

    2014-01-01

    Background and Objectives Although prasugrel allows for rapid and potent platelet inhibition, the efficacy and safety of lower doses of prasugrel for patients of East Asian ethnicity has not yet been investigated. We compared the effect of a lower loading dose (LD) of prasugrel with conventional LDs of clopidogrel and prasugrel in Korean patients. Subjects and Methods Forty-three Korean patients undergoing coronary angiography were enrolled in the study. Participants were randomly administered LDs of clopidogrel 600 mg, prasugrel 30 mg or prasugrel 60 mg prior to coronary angiography. Platelet reactivity was assessed at baseline and at the time of peak platelet inhibition using light transmission aggregometry (LTA), the VerifyNow assay, and multiple electrode aggregometry. Results Although baseline platelet reactivity between the groups showed no significant differences, at the time of peak platelet inhibition, the prasugrel 30 mg (18.9±10.0%) and 60 mg groups (13.8±10.8%) showed significantly more potent platelet inhibition than the clopidogrel 600 mg group (52.9±15.8%; p<0.001) by LTA. However, there were no significant differences between the prasugrel 30 mg and 60 mg groups (p=0.549). Conclusion The loading effect of prasugrel 30 mg was more potent than clopidogrel 600 mg and was not significantly different from prasugrel 60 mg. PMID:25469140

  8. Mismatch repair proficiency is not required for radioenhancement by gemcitabine

    SciTech Connect

    Bree, Chris van . E-mail: c.vanbree@amc.uva.nl; Rodermond, Hans M.; Vos, Judith de; Haveman, Jaap; Franken, Nicolaas

    2005-08-01

    Purpose: Mismatch repair (MMR) proficiency has been reported to either increase or decrease radioenhancement by 24-h incubations with gemcitabine. This study aimed to establish the importance of MMR for radioenhancement by gemcitabine after short-exposure, high-dose treatment and long-exposure, low-dose treatment. Methods and Materials: Survival of MMR-deficient HCT116 and MMR-proficient HCT116 + 3 cells was analyzed by clonogenic assays. Mild, equitoxic gemcitabine treatments (4 h, 0.1 {mu}M vs. 24 h, 6 nM) were combined with {gamma}-irradiation to determine the radioenhancement with or without recovery. Gemcitabine metabolism and cell-cycle effects were evaluated by high-performance liquid chromatography analysis and bivariate flow cytometry. Results: Radioenhancement after 4 h of 0.1 {mu}M of gemcitabine was similar in both cell lines, but the radioenhancement after 24 h of 6 nM of gemcitabine was reduced in MMR-proficient cells. No significant differences between both cell lines were observed in the gemcitabine metabolism or cell-cycle effects after these treatments. Gemcitabine radioenhancement after recovery was also lower in MMR-proficient cells than in MMR-deficient cells. Conclusion: Mismatch repair proficiency decreases radioenhancement by long incubations of gemcitabine but does not affect radioenhancement by short exposures to a clinically relevant gemcitabine dose. Our data suggest that MMR contributes to the recovery from gemcitabine treatment.

  9. APC mutations in colorectal tumors with mismatch repair deficiency.

    PubMed Central

    Huang, J; Papadopoulos, N; McKinley, A J; Farrington, S M; Curtis, L J; Wyllie, A H; Zheng, S; Willson, J K; Markowitz, S D; Morin, P; Kinzler, K W; Vogelstein, B; Dunlop, M G

    1996-01-01

    We have investigated the influence of genetic instability [replication error (RER) phenotype] on APC (adenomatous polyposis coli), a gene thought to initiate colorectal tumorigenesis. The prevalence of APC mutations was similar in RER and non-RER tumors, indicating that both tumor types share this step in neoplastic transformation. However, in a total of 101 sequenced mutations, we noted a substantial excess of APC frameshift mutations in the RER cases (70% in RER tumors versus 47% in non-RER tumors, P < 0.04). These frameshifts were characteristic of mutations arising in cells deficient in DNA mismatch repair, with a predilection for mononucleotide repeats in the RER tumors (P < 0.0002), particularly (A)n tracts (P < 0.00007). These findings suggest that the genetic instability that is reflected by the RER phenotype precedes, and is responsible for, APC mutation in RER large bowel tumors and have important implications for understanding the very earliest stages of neoplasia in patients with tumors deficient in mismatch repair. Images Fig. 2 PMID:8799152

  10. Visualization of mismatch repair complexes using fluorescence microscopy.

    PubMed

    Schmidt, Tobias T; Hombauer, Hans

    2016-02-01

    DNA mismatch repair (MMR) is a surveillance mechanism present in most living organisms, which repairs errors introduced by DNA polymerases. Importantly, loss of MMR function due to inactivating mutations and/or epigenetic silencing results in the accumulation of mutations and as consequence increased cancer susceptibility, as observed in Lynch syndrome patients. During the past decades important progress has been made in the MMR field resulting in the identification and characterization of essential MMR components, culminating in the in vitro reconstitution of 5' and 3' nick-directed MMR. However, several mechanistic aspects of the MMR reaction remain not fully understood, therefore alternative approaches and further investigations are needed. Recently, the use of imaging techniques and, more specifically, visualization of MMR components in living cells, has broadened our mechanistic understanding of the repair reaction providing more detailed information about the spatio-temporal organization of MMR in vivo. In this review we would like to comment on mechanistic aspects of the MMR reaction in light of these and other recent findings. Moreover, we will discuss the current limitations and provide future perspectives regarding imaging of mismatch repair components in diverse organisms. PMID:26725956

  11. Predictable patterns of trait mismatches between interacting plants and insects

    PubMed Central

    2010-01-01

    Background There are few predictions about the directionality or extent of morphological trait (mis)matches between interacting organisms. We review and analyse studies on morphological trait complementarity (e.g. floral tube length versus insect mouthpart length) at the population and species level. Results Plants have consistently more exaggerated morphological traits than insects at high trait magnitudes and in some cases less exaggerated traits than insects at smaller trait magnitudes. This result held at the population level, as well as for phylogenetically adjusted analyses at the species-level and for both pollination and host-parasite interactions, perhaps suggesting a general pattern. Across communities, the degree of trait mismatch between one specialist plant and its more generalized pollinator was related to the level of pollinator specialization at each site; the observed pattern supports the "life-dinner principle" of selection acting more strongly on species with more at stake in the interaction. Similarly, plant mating system also affected the degree of trait correspondence because selfing reduces the reliance on pollinators and is analogous to pollination generalization. Conclusions Our analyses suggest that there are predictable "winners" and "losers" of evolutionary arms races and the results of this study highlight the fact that breeding system and the degree of specialization can influence the outcome. PMID:20604973

  12. Mismatch characteristics of optical parametric chirped pulse amplification

    NASA Astrophysics Data System (ADS)

    Novák, O.; Turčičová, H.; Divoký, M.; Huynh, J.; Straka, P.

    2014-02-01

    The stability of an optical parametric chirped pulse amplifier (OPCPA) is influenced by time and the angular matching of the input beams. We derived the Gaussian dependence of the monochromatic signal gain on the small mismatch between the signal and pump beams. Gain characteristics were also calculated for polychromatic amplification and the impact of different beam mismatches and interaction geometries was explained. The asymmetry of the energy gain, and the square root dependence of the phase matched wavelength on beam angles were found. The predicted dependences were verified in a noncollinear OPCPA system with LBO and KDP crystal amplifying pulses of a Ti:sapphire laser around a central wavelength of 800 nm, pumped by the third harmonic frequency of an iodine gas laser at a wavelength of 438 nm. The widths of the gain curves in the dependence on both the pump-signal or the phase matching angles varied from several tenths to a few milliradians. The gain curve widths dependent on the pump-signal pulse delay were about two thirds of the pump pulse width for moderate pumping and about a half of the pump pulse width for pumping on the order of GW cm-2. A stable gain output is achieved if angular and temporal fluctuations are fractions of the measured gain curve widths, and when the signal direction is between the pump and the crystal principal axis (i.e. in the psz geometry).

  13. Halo Formation of a Mismatched Beam in an Induction LINAC

    NASA Astrophysics Data System (ADS)

    Chan, Chun Fai

    1996-11-01

    Beam halo formation and emittance growth due to initial envelope mismatch are studied with particle-in-cell simulation for a planned high current experiment with a 2 MeV, 0.8 amp K^+ beam. Transport over 15 m is provided by 55 sets of electrostatic quadrupoles and acceleration could be incorporated with induction modules at drift spaces. The tool used in the study is a (2+1)d code HIBEAM. Clearly visible beam halos are formed in the phase space and real space plots beyond 6 m, if the beam is initially mismatched in the envelope radius and angle by about 20% or more. Furthermore, we present the argument that the physical reason for the effect is due to successive aberration, described earlier by J.D. Lawson.(J.D. Lawson, The Physics of Charged-Particle Beams, p. 200, 1977.) Existing experimental data(M.G. Tiefenback, Proceedings of the 1987 Particle Accelerator Conference, p.1046, 1987.) of halo formation supports this interpretation.

  14. Predicting χ for polymers with stiffness mismatch from simulations

    NASA Astrophysics Data System (ADS)

    Kozuch, Daniel; Zhang, Wenlin; Gomez, Enrique; Milner, Scott

    The Flory-Huggins χ parameter describes the excess free energy of mixing and governs phase behavior for polymer blends and block copolymers. For chemically distinct polymers, the value of χ is dominated by the mismatch in cohesive energy densities of the monomers. For blends of chemically similar polymers, the entropic portion of χ, arising from non-ideal local packing, becomes more significant. Using polymer field theory, Fredrickson, Liu, and Bates predict that a difference in backbone stiffness can result in a positive χ for chains consisting of chemically identical monomers. To quantitatively investigate this phenomenon, we perform molecular dynamic (MD) simulations for bead-spring chains which differ only in stiffness. From the simulations, we apply a novel thermodynamic integration to extract χ as low as 10-3 per monomer for blends with mild stiffness mismatch. By introducing a standardized effective monomer, we map real polymers to our bead-spring chains and show that the predicted entropic portion of χ are consistent with experimental data.

  15. Delivery performance of conventional aircraft by terminal-area, time-based air traffic control: A real-time simulation evaluation

    NASA Technical Reports Server (NTRS)

    Credeur, Leonard; Houck, Jacob A.; Capron, William R.; Lohr, Gary W.

    1990-01-01

    A description and results are presented of a study to measure the performance and reaction of airline flight crews, in a full workload DC-9 cockpit, flying in a real-time simulation of an air traffic control (ATC) concept called Traffic Intelligence for the Management of Efficient Runway-scheduling (TIMER). Experimental objectives were to verify earlier fast-time TIMER time-delivery precision results and obtain data for the validation or refinement of existing computer models of pilot/airborne performance. Experimental data indicated a runway threshold, interarrival-time-error standard deviation in the range of 10.4 to 14.1 seconds. Other real-time system performance parameters measured include approach speeds, response time to controller turn instructions, bank angles employed, and ATC controller message delivery-time errors.

  16. Convention Problems - 1787.

    ERIC Educational Resources Information Center

    Hanson, Deroy L.

    Designed to motivate eighth-grade civics students in the study of the United States Constitution, this game is intended to simulate the basic problems faced by the delegates to the Philadelphia Convention of 1787. The four parts of the game introduce the governmental concepts of the bicameral legislature, the executive branch, the judicial branch,…

  17. Recombinant activated factor VII (rFVIIa) as a hemostatic agent in liver disease: a break from convention in need of controlled trials.

    PubMed

    Caldwell, Stephen H; Chang, Charissa; Macik, B Gail

    2004-03-01

    The management of coagulopathy in patients with acute and chronic liver disease has undergone little change in many years despite advances in our understanding of the pathogenesis of this problem. In general, deficiency of clotting factors as a result of poor hepatic synthetic function accounts for most of the coagulopathy. However, other processes such as disseminated intravascular coagulation (DIC), hyperfibrinolysis, dysfibrinogenemia, hemolysis, and a decrease in number or function of platelets may be present and thus add to the complexity of the problem. Coexisting portal hypertension and the associated risks of volume expansion, renal failure, and endothelial dysfunction add even more difficulty to the management of these patients. The clinician's despair is only exacerbated by uncertainty regarding the significance of laboratory indices of coagulation and the lack of agreement between health care providers regarding how to use these indices. Simple, conventional interventions such as vitamin K or plasma administration often produce only limited amelioration, and the latter carries the potential disadvantage of volume overexpansion as well as the risk of infection and transfusion reactions. Into this complex and uncertain clinical situation has arrived the antihemophilic agent recombinant activated factor VII (rFVIIa). Its development has led to a fundamental re-evaluation of the classic understanding of the normal clotting cascade. Moreover, use of this product in liver disease patients is increasing despite the lack of definitive studies or literature to guide therapy. Herein we review the mechanism of action of this agent, report the clinical applications in patients with liver disease, address the limitations and risks associated with the drug, and discuss the issue of its cost-effectiveness. PMID:14999675

  18. Marrow Ablative and Immunosuppressive Effects of I-131-anti-CD45 Antibody in Congenic and H2-Mismatched Murine Transplant Models

    SciTech Connect

    Matthews, D. C.; Martin, P J.; Nourigat, C.; Appelbaum, F. R.; Fisher, Darrell R. ); Bernstein, I. D.

    1998-12-01

    Targeted hematopoietic irradiation delivered by I-131-anti-CD45 antibody has been combined with conventional marrow transplant preparative regimens in an effort to decrease relapse. Before increasing the proportion of therapy delivered by radiolabeled antibody, the myeloablative and immunosuppressive effects of such low dose rate irradiation must be quantitated. We have examined the ability of I-131-anti-CD45 antibody to facilitate engraftment in Ly5-congenic and H2-mismatched murine marrow transplant models. Recipient B6-Ly5-a mice were treated with 30F11 antibody labeled with 0.1 to 1.5 mCi I-131 and/or total body irradiation (TBI), followed by T-cell-depleted marrow from Ly5-b-congenic (C57BL/6) or H2-mismatched (BALB/c) donors. Engraftment was achieved readily in the Ly5-congenic setting, with greater than 80% donor granulocytes and T cells after 0.5 mCi I-131 (estimated 17 Gy to marrow) or 8 Gy TBI. A higher TBI dose (14 Gy) was required to achieve engraftment of H2-mismatched mar row, and engraftment occurred in only 3 of 11 mice receiving 1.5 mCi I-131 delivered by anti-CD45 antibody. Engraftment of H2-mismatched marrow was achieved in 22 of 23 animals receiving 0.75 mCi I-131 delivered by anti-CD45 antibody combined with 8 Gy TBI. Thus, targeted radiation delivered via I-131-anti-CD45 antibody can enable engraftment of congenic marrow and can partially replace TBI when transplanting T-cell-depleted H2-mismatched marrow.

  19. A technique to consider mismatches between fMRI and EEG/MEG sources for fMRI-constrained EEG/MEG source imaging: a preliminary simulation study

    NASA Astrophysics Data System (ADS)

    Im, Chang-Hwan; Lee, Soo Yeol

    2006-12-01

    fMRI-constrained EEG/MEG source imaging can be a powerful tool in studying human brain functions with enhanced spatial and temporal resolutions. Recent studies on the combination of fMRI and EEG/MEG have suggested that fMRI prior information could be readily implemented by simply imposing different weighting factors to cortical sources overlapping with the fMRI activations. It has been also reported, however, that such a hard constraint may cause severe distortions or elimination of meaningful EEG/MEG sources when there are distinct mismatches between the fMRI activations and the EEG/MEG sources. If one wants to obtain the actual EEG/MEG source locations and uses the fMRI prior information as just an auxiliary tool to enhance focality of the distributed EEG/MEG sources, it is reasonable to weaken the strength of fMRI constraint when severe mismatches between fMRI and EEG/MEG sources are observed. The present study suggests an efficient technique to automatically adjust the strength of fMRI constraint according to the mismatch level. The use of the proposed technique rarely affects the results of conventional fMRI-constrained EEG/MEG source imaging if no major mismatch between the two modalities is detected; while the new results become similar to those of typical EEG/MEG source imaging without fMRI constraint if the mismatch level is significant. A preliminary simulation study using realistic EEG signals demonstrated that the proposed technique can be a promising tool to selectively apply fMRI prior information to EEG/MEG source imaging.

  20. Key Rate Available from Mismatched Measurements in the BB84 Protocol and the Uncertainty Principle

    NASA Astrophysics Data System (ADS)

    Matsumoto, Ryutaroh; Watanabe, Shun

    We consider the mismatched measurements in the BB84 quantum key distribution protocol, in which measuring bases are different from transmitting bases. We give a lower bound on the amount of a secret key that can be extracted from the mismatched measurements. Our lower bound shows that we can extract a secret key from the mismatched measurements with certain quantum channels, such as the channel over which the Hadamard matrix is applied to each qubit with high probability. Moreover, the entropic uncertainty principle implies that one cannot extract the secret key from both matched measurements and mismatched ones simultaneously, when we use the standard information reconciliation and privacy amplification procedure.

  1. Kinetics and binding of the thymine-DNA mismatch glycosylase, Mig-Mth, with mismatch-containing DNA substrates.

    PubMed

    Begley, Thomas J; Haas, Brian J; Morales, Juan C; Kool, Eric T; Cunningham, Richard P

    2003-01-01

    We have examined the removal of thymine residues from T-G mismatches in DNA by the thymine-DNA mismatch glycosylase from Methanobacterium thermoautrophicum (Mig-Mth), within the context of the base excision repair (BER) pathway, to investigate why this glycosylase has such low activity in vitro. Using single-turnover kinetics and steady-state kinetics, we calculated the catalytic and product dissociation rate constants for Mig-Mth, and determined that Mig-Mth is inhibited by product apyrimidinic (AP) sites in DNA. Electrophoretic mobility shift assays (EMSA) provide evidence that the specificity of product binding is dependent upon the base opposite the AP site. The binding of Mig-Mth to DNA containing the non-cleavable substrate analogue difluorotoluene (F) was also analyzed to determine the effect of the opposite base on Mig-Mth binding specificity for substrate-like duplex DNA. The results of these experiments support the idea that opposite strand interactions play roles in determining substrate specificity. Endonuclease IV, which cleaves AP sites in the next step of the BER pathway, was used to analyze the effect of product removal on the overall rate of thymine hydrolysis by Mig-Mth. Our results support the hypothesis that endonuclease IV increases the apparent activity of Mig-Mth significantly under steady-state conditions by preventing reassociation of enzyme to product. PMID:12509271

  2. Temporal characterization of protein production levels from baculovirus vectors coding for GFP and RFP genes under non-conventional promoter control.

    PubMed

    George, Steve; Jauhar, Altamash M; Mackenzie, Jennifer; Kieβlich, Sascha; Aucoin, Marc G

    2015-09-01

    The ease of use and versatility of the Baculovirus Expression Vector System (BEVS) has made it one of the most widely used systems for recombinant protein production However, co-expression systems currently in use mainly make use of the very strong very late p10 and polyhedron (polh) promoters to drive expression of foreign genes, which does not provide much scope for tailoring expression ratios within the cell. This work demonstrates the use of different Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) promoters to control the timing and expression of two easily traceable fluorescent proteins, the enhanced green fluorescent protein (eGFP), and a red fluorescent protein (DsRed2) in a BEVS co-expression system. Our results show that gene expression levels can easily be controlled using this strategy, and also that modulating the expression level of one protein can influence the level of expression of the other protein within the system, thus confirming the concept of genes "competing" for limited cellular resources. Plots of "expression ratios" of the two model genes over time were obtained, and may be used in future work to tightly control timing and levels of foreign gene expression in an insect cell co-expression system. PMID:25850946

  3. The INCH-Trial: a multicentre randomized controlled trial comparing the efficacy of conventional open surgery and laparoscopic surgery for incisional hernia repair

    PubMed Central

    2013-01-01

    Background Annually approximately 100.000 patients undergo a laparotomy in the Netherlands. About 15,000 of these patients will develop an incisional hernia. Both open and laparoscopic surgical repair have been proven to be safe. However, the most effective treatment of incisional hernias remains unclear. This study, the ‘INCH-trial’, comparing cost-effectiveness of open and laparoscopic incisional hernia repair, is therefore needed. Methods/Design A randomized multi-center clinical trial comparing cost-effectiveness of open and laparoscopic repair of incisional hernias. Patients with a symptomatic incisional hernia, eligible for laparoscopic and open incisional hernia repair. Only surgeons, experienced in both open and laparoscopic incisional hernia repair, will participate in the INCH trial. During incisional hernia repair, a mesh is placed under or on top of the fascia, with a minimal overlap of 5 cm. Primary endpoint is length of hospital stay after an incisional hernia repair. Secondary endpoints are time to full recovery within three months after index surgery, post-operative complications, recurrences, mortality and quality of life. Our hypothesis is that laparoscopic incisional hernia repair comes with a significant shorter hospital stay compared to open incisional hernia repair. A difference of two days is considered significant. One-hunderd-and-thirty-five patients are enrolled in each treatment arm. The economic evaluation will be performed from a societal perspective. Primary outcomes are costs per patient related to time-to-recovery and quality of life. The main goal of the trial is to establish whether laparoscopic incisional hernia repair is superior to conventional open incisional hernia repair in terms of cost-effectiveness. This is measured through length of hospital stay and quality of life. Secondary endpoints are re-operation rate due to post-operative complications or recurrences, mortality and quality of life. Discussion The difference

  4. Atypical mismatch negativity in response to emotional voices in people with autism spectrum conditions.

    PubMed

    Fan, Yang-Teng; Cheng, Yawei

    2014-01-01

    Autism Spectrum Conditions (ASC) are characterized by heterogeneous impairments of social reciprocity and sensory processing. Voices, similar to faces, convey socially relevant information. Whether voice processing is selectively impaired remains undetermined. This study involved recording mismatch negativity (MMN) while presenting emotionally spoken syllables dada and acoustically matched nonvocal sounds to 20 subjects with ASC and 20 healthy matched controls. The people with ASC exhibited no MMN response to emotional syllables and reduced MMN to nonvocal sounds, indicating general impairments of affective voice and acoustic discrimination. Weaker angry MMN amplitudes were associated with more autistic traits. Receiver operator characteristic analysis revealed that angry MMN amplitudes yielded a value of 0.88 (p<.001). The results suggest that people with ASC may process emotional voices in an atypical fashion already at the automatic stage. This processing abnormality can facilitate diagnosing ASC and enable social deficits in people with ASC to be predicted. PMID:25036143

  5. Lattice-Mismatched III-V Epilayers for High-Efficiency Photovoltaics

    SciTech Connect

    Ahrenkiel, Scott Phillip

    2013-06-30

    The project focused on development of new approaches and materials combinations to expand and improve the quality and versatility of lattice-mismatched (LMM) III-V semiconductor epilayers for use in high-efficiency multijunction photovoltaic (PV) devices. To address these goals, new capabilities for materials synthesis and characterization were established at SDSM&T that have applications in modern opto- and nano-electronics, including epitaxial crystal growth and transmission electron microscopy. Advances were made in analyzing and controlling the strain profiles and quality of compositional grades used for these technologies. In particular, quaternary compositional grades were demonstrated, and a quantitative method for characteristic X-ray analysis was developed. The project allowed enhanced collaboration between scientists at NREL and SDSM&T to address closely related research goals, including materials exchange and characterization.

  6. Lattice-mismatch-induced twinning for seeded growth of anisotropic nanostructures.

    PubMed

    Wang, Zhenni; Chen, Zhengzheng; Zhang, Hui; Zhang, Zhaorui; Wu, Haijun; Jin, Mingshang; Wu, Chao; Yang, Deren; Yin, Yadong

    2015-03-24

    Synthesis of anisotropic nanostructures from materials with isotropic crystal structures often requires the use of seeds containing twin planes to break the crystalline symmetry and promote the preferential anisotropic growth. Controlling twinning in seeds is therefore critically important for high-yield synthesis of many anisotropic nanostructures. Here, we demonstrate a unique strategy to induce twinning in metal nanostructures for anisotropic growth by taking advantage of the large lattice mismatch between two metals. By using Au-Cu as an example, we show, both theoretically and experimentally, that deposition of Cu to the surface of single-crystalline Au seeds can build up strain energy, which effectively induces the formation of twin planes. Subsequent seeded growth allows the production of Cu nanorods with high shape anisotropy that is unachievable without the use of Au seeds. This work provides an effective strategy for the preparation of anisotropic metal nanostructures. PMID:25744113

  7. Somatic aphasia: Mismatch of body sensations with autonomic stress reactivity in psychopathy

    PubMed Central

    Gao, Yu; Raine, Adrian; Schug, Robert A.

    2012-01-01

    Background Although one of the main characteristics of psychopaths is a deficit in emotion, it is unknown whether they show a fundamental impairment in appropriately recognizing their own body sensations during an emotion-inducing task. Method Skin conductance and heart rate were recorded in 138 males during a social stressor together with subjective reports of body sensations. Psychopathic traits were assessed using the Psychopathy Checklist – Revised (PCL-R) 2nd edition (Hare, 2003). Results Nonpsychopathic controls who reported higher body sensations showed higher heart rate reactivity, but this verbal-autonomic consistency was not found in psychopathic individuals. This mind-body disconnection is particularly associated with the interpersonal-affective factor of psychopathy. Conclusions Findings are the first to document this body sensations– autonomic mismatch in psychopaths, and suggest that somatic aphasia the inaccurate identification and recognition of one‘s own somatic states may partly underlie the interpersonal-affective features of psychopaths. PMID:22490763

  8. Is it time to move mismatch negativity into the clinic?

    PubMed

    Schall, Ulrich

    2016-04-01

    Since its inception in the 1970s, the mismatch negativity (MMN) event-related potential has improved our understanding of pre-attentive detection of rule violations, which is a fundamental cognitive process considered by some a form of "primitive intelligence". The body of research to date ranges from animal studies (i.e. when investigating the neural mechanisms and pharmacological properties of MMN generation) to researching the psychophysiological nature of human consciousness. MMN therefore offers the possibility to detect abnormal functioning in the neural system involved in MMN generation, such as it occurs in some neurodevelopmental disorders or patients in vegetative state. While the clinical research data holds considerable promise for translation into clinical practice, standardization and normative data of an optimized (i.e. disorder-specific) MMN recording algorithm is needed in order for MMN to become a valuable clinical investigation tool. PMID:26342995

  9. DNA mismatch repair: molecular mechanisms and biological function.

    PubMed

    Schofield, Mark J; Hsieh, Peggy

    2003-01-01

    DNA mismatch repair (MMR) guards the integrity of the genome in virtually all cells. It contributes about 1000-fold to the overall fidelity of replication and targets mispaired bases that arise through replication errors, during homologous recombination, and as a result of DNA damage. Cells deficient in MMR have a mutator phenotype in which the rate of spontaneous mutation is greatly elevated, and they frequently exhibit microsatellite instability at mono- and dinucleotide repeats. The importance of MMR in mutation avoidance is highlighted by the finding that defects in MMR predispose individuals to hereditary nonpolyposis colorectal cancer. In addition to its role in postreplication repair, the MMR machinery serves to police homologous recombination events and acts as a barrier to genetic exchange between species. PMID:14527292

  10. The mismatch negativity: a review of underlying mechanisms.

    PubMed

    Garrido, Marta I; Kilner, James M; Stephan, Klaas E; Friston, Karl J

    2009-03-01

    The mismatch negativity (MMN) is a brain response to violations of a rule, established by a sequence of sensory stimuli (typically in the auditory domain) [Näätänen R. Attention and brain function. Hillsdale, NJ: Lawrence Erlbaum; 1992]. The MMN reflects the brain's ability to perform automatic comparisons between consecutive stimuli and provides an electrophysiological index of sensory learning and perceptual accuracy. Although the MMN has been studied extensively, the neurophysiological mechanisms underlying the MMN are not well understood. Several hypotheses have been put forward to explain the generation of the MMN; amongst these accounts, the "adaptation hypothesis" and the "model adjustment hypothesis" have received the most attention. This paper presents a review of studies that focus on neuronal mechanisms underlying the MMN generation, discusses the two major explanatory hypotheses, and proposes predictive coding as a general framework that attempts to unify both. PMID:19181570

  11. Mismatch Negativity with Visual-only and Audiovisual Speech

    PubMed Central

    Ponton, Curtis W.; Bernstein, Lynne E.; Auer, Edward T.

    2009-01-01

    The functional organization of cortical speech processing is thought to be hierarchical, increasing in complexity and proceeding from primary sensory areas centrifugally. The current study used the mismatch negativity (MMN) obtained with electrophysiology (EEG) to investigate the early latency period of visual speech processing under both visual-only (VO) and audiovisual (AV) conditions. Current density reconstruction (CDR) methods were used to model the cortical MMN generator locations. MMNs were obtained with VO and AV speech stimuli at early latencies (approximately 82-87 ms peak in time waveforms relative to the acoustic onset) and in regions of the right lateral temporal and parietal cortices. Latencies were consistent with bottom-up processing of the visible stimuli. We suggest that a visual pathway extracts phonetic cues from visible speech, and that previously reported effects of AV speech in classical early auditory areas, given later reported latencies, could be attributable to modulatory feedback from visual phonetic processing. PMID:19404730

  12. Deterministic coherence resonance in coupled chaotic oscillators with frequency mismatch

    NASA Astrophysics Data System (ADS)

    Pisarchik, A. N.; Jaimes-Reátegui, R.

    2015-11-01

    A small mismatch between natural frequencies of unidirectionally coupled chaotic oscillators can induce coherence resonance in the slave oscillator for a certain coupling strength. This surprising phenomenon resembles "stabilization of chaos by chaos," i.e., the chaotic driving applied to the chaotic system makes its dynamics more regular when the natural frequency of the slave oscillator is a little different than the natural frequency of the master oscillator. The coherence is characterized with the dominant component in the power spectrum of the slave oscillator, normalized standard deviations of both the peak amplitude and the interpeak interval, and Lyapunov exponents. The enhanced coherence is associated with increasing negative both the third and the fourth Lyapunov exponents, while the first and second exponents are always positive and zero, respectively.

  13. Deterministic coherence resonance in coupled chaotic oscillators with frequency mismatch.

    PubMed

    Pisarchik, A N; Jaimes-Reátegui, R

    2015-11-01

    A small mismatch between natural frequencies of unidirectionally coupled chaotic oscillators can induce coherence resonance in the slave oscillator for a certain coupling strength. This surprising phenomenon resembles "stabilization of chaos by chaos," i.e., the chaotic driving applied to the chaotic system makes its dynamics more regular when the natural frequency of the slave oscillator is a little different than the natural frequency of the master oscillator. The coherence is characterized with the dominant component in the power spectrum of the slave oscillator, normalized standard deviations of both the peak amplitude and the interpeak interval, and Lyapunov exponents. The enhanced coherence is associated with increasing negative both the third and the fourth Lyapunov exponents, while the first and second exponents are always positive and zero, respectively. PMID:26651632

  14. Mismatch Repair and Colon Cancer: Mechanisms and Therapies Explored.

    PubMed

    Li, Stephen K H; Martin, Alberto

    2016-04-01

    Colorectal cancer (CRC) remains one of the most prevalent cancers worldwide. In sporadic CRC, mutations frequently occur in the DNA mismatch repair (MMR) pathway. In addition, germline MMR mutations have been linked to Lynch syndrome, the most common form of hereditary CRC. Although genetic mutations, diet, inflammation, and the gut microbiota can influence CRC, it is unclear how MMR deficiency relates to these factors to modulate disease. In this review, the association of MMR to the etiology of CRC is examined, particularly in the context of microRNAs (miRNAs), inflammation, and the microbiome. We also discuss the most current targeted therapies, methods of prevention, and molecular biomarkers against MMR-deficient CRC, all of which are encouraging advancements in the field. PMID:26970951

  15. Effect of material mismatch on dynamic failure of interfaces

    SciTech Connect

    Singh, R.P.; Shukla, A.

    1995-12-31

    An experimental study has been conducted to study the effect of mismatch of material properties on the dynamic failure of interfaces. The bimaterial specimens are fabricated using various material systems with different elastic properties. The specimen is then loaded dynamically by impacting it with a projectile fired from a pressurized gas gun. This impact results in a compressive wave which traverses the width of the specimen and reflects from the free surface as a tensile stress wave pulse. The tensile loading of the crack tip results in fracture initiation and subsequent crack growth along the interface. This entire event is observed using dynamic photoelasticity in conjunction with high speed photography. The isochromatic fringe patterns obtained using dynamic photoelasticity provide full field information about the dynamic fracture process. Location of the crack tip as a function of time is determined directly from these isochromatic fringes. These data are differentiated with respect to time to provide the velocity of the dynamically propagating interface crack. Typically, the moving crack tip attains very high velocities, which exceed the shear wave velocity of the more compliant material comprising the interface. This exceeding of the shear wave velocity results in the formation of shock patterns ahead of the crack tip. In addition to determining the crack tip location, the isochromatic fringes also provide full field information about the stress field surrounding the dynamically propagating crack tip. The photoelastic information is analyzed to determine various fracture parameters including the complex stress intensity factor and the non-singular terms. The analysis is based on the higher order asymptotic field equations. The variation in the fracture parameters as a function of crack velocity, crack position and mismatch of material properties will be presented and discussed at the meeting.

  16. Single-base-pair discrimination of terminal mismatches by using oligonucleotide microarrays and neural network analyses

    NASA Technical Reports Server (NTRS)

    Urakawa, Hidetoshi; Noble, Peter A.; El Fantroussi, Said; Kelly, John J.; Stahl, David A.

    2002-01-01

    The effects of single-base-pair near-terminal and terminal mismatches on the dissociation temperature (T(d)) and signal intensity of short DNA duplexes were determined by using oligonucleotide microarrays and neural network (NN) analyses. Two perfect-match probes and 29 probes having a single-base-pair mismatch at positions 1 to 5 from the 5' terminus of the probe were designed to target one of two short sequences representing 16S rRNA. Nonequilibrium dissociation rates (i.e., melting profiles) of all probe-target duplexes were determined simultaneously. Analysis of variance revealed that position of the mismatch, type of mismatch, and formamide concentration significantly affected the T(d) and signal intensity. Increasing the concentration of formamide in the washing buffer decreased the T(d) and signal intensity, and it decreased the variability of the signal. Although T(d)s of probe-target duplexes with mismatches in the first or second position were not significantly different from one another, duplexes with mismatches in the third to fifth positions had significantly lower T(d)s than those with mismatches in the first or second position. The trained NNs predicted the T(d) with high accuracies (R(2) = 0.93). However, the NNs predicted the signal intensity only moderately accurately (R(2) = 0.67), presumably due to increased noise in the signal intensity at low formamide concentrations. Sensitivity analysis revealed that the concentration of formamide explained most (75%) of the variability in T(d)s, followed by position of the mismatch (19%) and type of mismatch (6%). The results suggest that position of the mismatch at or near the 5' terminus plays a greater role in determining the T(d) and signal intensity of duplexes than the type of mismatch.

  17. Detection and Quantification of Plectosphaerella cucumerina, a Potential Biological Control Agent of Potato Cyst Nematodes, by Using Conventional PCR, Real-Time PCR, Selective Media, and Baiting

    PubMed Central

    Atkins, S. D.; Clark, I. M.; Sosnowska, D.; Hirsch, P. R.; Kerry, B. R.

    2003-01-01

    Potato cyst nematodes (PCN) are serious pests in commercial potato production, causing yield losses valued at approximately $300 million in the European Community. The nematophagous fungus Plectosphaerella cucumerina has demonstrated its potential as a biological control agent against PCN populations by reducing field populations by up to 60% in trials. The use of biological control agents in the field requires the development of specific techniques to monitor the release, population size, spread or decline, and pathogenicity against its host. A range of methods have therefore been developed to monitor P. cucumerina. A species-specific PCR primer set (PcCF1-PcCR1) was designed that was able to detect the presence of P. cucumerina in soil, root, and nematode samples. PCR was combined with a bait method to identify P. cucumerina from infected nematode eggs, confirming the parasitic ability of the fungus. A selective medium was adapted to isolate the fungus from root and soil samples and was used to quantify the fungus from field sites. A second P. cucumerina-specific primer set (PcRTF1-PcRTR1) and a Taqman probe (PcRTP1) were designed for real-time PCR quantification of the fungus and provided a very sensitive means of detecting the fungus from soil. PCR, bait, and culture methods were combined to investigate the presence and abundance of P. cucumerina from two field sites in the United Kingdom where PCN populations were naturally declining. All methods enabled differences in the activity of P. cucumerina to be detected, and the results demonstrated the importance of using a combination of methods to investigate population size and activity of fungi. PMID:12902272

  18. Conventional treatments for ankylosing spondylitis

    PubMed Central

    Dougados, M; Dijkmans, B; Khan, M; Maksymowych, W; van der Linden, S.; Brandt, J

    2002-01-01

    Management of ankylosing spondylitis (AS) is challenged by the progressive nature of the disease. To date, no intervention is available that alters the underlying mechanism of inflammation in AS. Currently available conventional treatments are palliative at best, and often fail to control symptoms in the long term. Current drug treatment may perhaps induce a spurious state of "disease remission," which is merely a low level of disease activity. Non-steroidal anti-inflammatory drugs are first line treatment, but over time, the disease often becomes refractory to these agents. Disease modifying antirheumatic drugs are second line treatment and may offer some clinical benefit. However, conclusive evidence of the efficacy of these drugs from large placebo controlled trials is lacking. Additionally, these drugs can cause treatment-limiting adverse effects. Intra-articular corticosteroid injection guided by arthrography, computed tomography, or magnetic resonance imaging is an effective means of reducing inflammatory back pain, but controlled studies are lacking. A controlled study has confirmed moderate but significant efficacy of intravenous bisphosphonate (pamidronate) treatment in patients with AS; further evaluation of bisphosphonate treatment is warranted. Physical therapy and exercise are necessary adjuncts to pharmacotherapy; however, the paucity of controlled data makes it difficult to identify the best way to administer these interventions. Surgical intervention may be required to support severe structural damage. Thus, for patients with AS, the future of successful treatment lies in the development of pharmacological agents capable of both altering the disease course through intervention at sites of disease pathogenesis, and controlling symptoms. PMID:12381510

  19. Changes proposed to improve leak resistance of casing due to mismatching thread leads

    SciTech Connect

    Blose, T.L.

    1984-01-23

    The leak resistance of the API buttress casing connection can be improved by mismatching the thread leads between coupling and casing members. These modifications can be made such that the improved product will meet all present API standards and will not affect interchangeability with present products or require changes in gages or tooling. The buttress thread casing connection is one of the most widely used oil well casing connections. It is now an American Petroleum Institute (API) standard connection. It is listed in API Standard 5B and titled ''Buttress Thread Casing''. This connection was patented by Samuel Webb in 1956, assigned to United States Steel. The purpose of the invention was to develop a threaded tubular connection as strong as the pipe body in axial tension without having to upset the tube ends. The inventor also recognized that a connection needed to be leak tight even when carrying high tensile loads. The patent states, ''There has also been a tendency for conventional joints to leak when subjected to high pressures when bearing heavy loads and it is imperative that the casing joints do not leak under such conditions''. This latter point is the subject of this article.

  20. Valve-in-valve transcatheter aortic valve implantation: the new playground for prosthesis-patient mismatch.

    PubMed

    Faerber, Gloria; Schleger, Simone; Diab, Mahmoud; Breuer, Martin; Figulla, Hans R; Eichinger, Walter B; Doenst, Torsten

    2014-06-01

    Transcatheter aortic valve implantation (TAVI) has become an established procedure for patients with aortic valve stenosis and significant comorbidities. One option offered by this technique is the implantation of a transcatheter valve inside a surgically implanted bioprosthesis. Many reports address the feasibility but also the pitfalls of these valve-in-valve (VIV) procedures. Review articles provide tables listing which valve sizes are appropriate based on the size of the initially implanted bioprosthesis. However, we previously argued that the hemodynamic performance of a prosthetic tissue valve is in large part a result of the dimensions of the bioprosthesis in relation to the patient's aortic outflow dimensions. Thus, the decision if a VIV TAVI procedure is likely to be associated with a favorable hemodynamic result cannot safely be made by looking at premade sizing tables that do not include patient dimensions and do not inquire about the primary cause for bioprosthetic valve stenosis. Prosthesis-patient mismatch (PPM) may therefore be more frequent than expected after conventional aortic valve replacement. Importantly, it may be masked by a potentially flawed method assessing its relevance. Such PPM may therefore impact significantly on hemodynamic outcome after VIV TAVI. Fifteen percent of currently published VIV procedures show only a minimal reduction of pressure gradients. We will address potential pitfalls in the current determination of PPM, outline the missing links for reliable determination of PPM, and present a simplified algorithm to guide decision making for VIV TAVI. PMID:24612128

  1. Group velocity mismatch-absent nonlinear frequency conversions for mid-infrared femtosecond pulses generation

    PubMed Central

    Zhong, Haizhe; Zhang, Lifu; Li, Ying; Fan, Dianyuan

    2015-01-01

    A novel group velocity mismatch (GVM) absent scheme for nonlinear optical parametric procedure in mid-infrared was developed with type-I quasi phase matching by use of an off-digital nonlinear optical coefficient d31. This was achieved by matching of the group velocities of the pump and the signal waves, while the phase velocities were quasi phase matched. The system employs MgO-doped periodically poled LiNbO3 as the nonlinear medium. Desired group-velocity dispersion would be obtained via appropriately temperature regulation. To demonstrate its potential applications in ultrafast mid-infrared pulses generation, aiming at a typical mid-infrared wavelength of ~3.2 μm, design examples of two basic nonlinear frequency conversion procedures are studied for both the narrow-band seeding mid-IR optical parametric amplification (OPA) and the synchronously pumped femtosecond optical parametric oscillation (SPOPO). Compared with the conventional scheme of type-0 QPM, the quantum-efficiency can be more than doubled with nearly unlimited bandwidth. The proposed GVM- absent phase matching design may provide a promising route to efficient and broadband sub-100 fs mid-infrared ultrafast pulses generation without group-velocity walk-off. PMID:26099837

  2. Single-molecule detection and mismatch discrimination of unlabeled DNA targets.

    PubMed

    Gunnarsson, Anders; Jönsson, Peter; Marie, Rodolphe; Tegenfeldt, Jonas O; Höök, Fredrik

    2008-01-01

    We report on a single-molecule readout scheme on total internal reflection fluorescence microscopy (TIRFM) demonstrating a detection limit in the low fM regime for short (30-mer) unlabeled DNA strands. Detection of unlabeled DNA targets is accomplished by letting them mediate the binding of suspended fluorescently labeled DNA-modified small unilamellar vesicles (Ø approximately 100 nm) to a DNA-modified substrate. On top of rapid and sensitive detection, the technique is also shown capable of extracting kinetics data from statistics of the residence time of the binding reaction in equilibrium, that is, without following neither the rate of binding upon injection nor release upon rinsing. The potential of this feature is demonstrated by discriminating a single mismatch from a fully complementary sequence. The success of the method is critically dependent on a surface modification that provides sufficiently low background. This was achieved through self-assembly of a biotinylated copolymer, Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) on a silicon dioxide surface, followed by subsequent addition of streptavidin and biotinylated DNA. The proposed detection scheme is particularly appealing due to the simplicity of the sensor, which relies on self-assembly principles and conventional TIRFM. Therefore, we foresee a great potential of the concept to serve as an important component in future multiplexed sensing schemes. This holds in particular true in cases when information about binding kinetics is valuable, such as in single nucleotide polymorphism diagnostics. PMID:18088151

  3. Global Adult Tobacco Survey data as a tool to monitor the WHO Framework Convention on Tobacco Control (WHO FCTC) implementation: the Brazilian case.

    PubMed

    Almeida, Liz; Szklo, André; Sampaio, Mariana; Souza, Mirian; Martins, Luís Felipe; Szklo, Moysés; Malta, Deborah; Caixeta, Roberta

    2012-07-01

    The Global Adult Tobacco Survey (GATS) was conducted in Brazil to provide data on tobacco use in order to monitor the WHO FCTC implementation in the country. It was carried out in 2008 using an international standardized methodology. The instrument included questions about tobacco use prevalence, cessation, secondhand smoke, knowledge, attitudes, media and advertising. Weighted analysis was used to obtain estimates. A total of 39,425 interviews were conducted. The prevalence of current tobacco use was 17.5%, (22.0%, men; 13.3%, women). The majority of users were smokers (17.2%) and their percentage was higher in rural areas (20.4%) than in urban areas (16.6%). About 20% of individuals reported having been exposed to tobacco smoke in public places. Over 70% of respondents said they had noticed anti-smoking information in several media and around 65% of smokers said they had considered quitting because of warning labels. About 30% of respondents had noticed cigarette advertising at selling points and 96% recognized tobacco use as a risk factor for serious diseases. Data in this report can be used as baseline for evaluation of new tobacco control approaches in Brazil, vis-à-vis WHO FCTC demand reduction measures. PMID:22851957

  4. Global Adult Tobacco Survey Data as a Tool to Monitor the WHO Framework Convention on Tobacco Control (WHO FCTC) Implementation: The Brazilian Case

    PubMed Central

    Almeida, Liz; Szklo, André; Sampaio, Mariana; Souza, Mirian; Martins, Luís Felipe; Szklo, Moysés; Malta, Deborah; Caixeta, Roberta

    2012-01-01

    The Global Adult Tobacco Survey (GATS) was conducted in Brazil to provide data on tobacco use in order to monitor the WHO FCTC implementation in the country. It was carried out in 2008 using an international standardized methodology. The instrument included questions about tobacco use prevalence, cessation, secondhand smoke, knowledge, attitudes, media and advertising. Weighted analysis was used to obtain estimates. A total of 39,425 interviews were conducted. The prevalence of current tobacco use was 17.5%, (22.0%, men; 13.3%, women). The majority of users were smokers (17.2%) and their percentage was higher in rural areas (20.4%) than in urban areas (16.6%). About 20% of individuals reported having been exposed to tobacco smoke in public places. Over 70% of respondents said they had noticed anti-smoking information in several media and around 65% of smokers said they had considered quitting because of warning labels. About 30% of respondents had noticed cigarette advertising at selling points and 96% recognized tobacco use as a risk factor for serious diseases. Data in this report can be used as baseline for evaluation of new tobacco control approaches in Brazil, vis-à-vis WHO FCTC demand reduction measures. PMID:22851957

  5. Quality of Survival and Growth in Children and Young Adults in the PNET4 European Controlled Trial of Hyperfractionated Versus Conventional Radiation Therapy for Standard-Risk Medulloblastoma

    SciTech Connect

    Kennedy, Colin; Bull, Kim; Chevignard, Mathilde; Culliford, David; Dörr, Helmuth G.; Doz, François; Kortmann, Rolf-Dieter; Lannering, Birgitta; Massimino, Maura; Navajas Gutiérrez, Aurora; Rutkowski, Stefan; Spoudeas, Helen A.; Calaminus, Gabriele

    2014-02-01

    Purpose: To compare quality of survival in “standard-risk” medulloblastoma after hyperfractionated radiation therapy of the central nervous system with that after standard radiation therapy, combined with a chemotherapy regimen common to both treatment arms, in the PNET4 randomised controlled trial. Methods and Materials: Participants in the PNET4 trial and their parents/caregivers in 7 participating anonymized countries completed standardized questionnaires in their own language on executive function, health status, behavior, health-related quality of life, and medical, educational, employment, and social information. Pre- and postoperative neurologic status and serial heights and weights were also recorded. Results: Data were provided by 151 of 244 eligible survivors (62%) at a median age at assessment of 15.2 years and median interval from diagnosis of 5.8 years. Compared with standard radiation therapy, hyperfractionated radiation therapy was associated with lower (ie, better) z-scores for executive function in all participants (mean intergroup difference 0.48 SDs, 95% confidence interval 0.16-0.81, P=.004), but health status, behavioral difficulties, and health-related quality of life z-scores were similar in the 2 treatment arms. Data on hearing impairment were equivocal. Hyperfractionated radiation therapy was also associated with greater decrement in height z-scores (mean intergroup difference 0.43 SDs, 95% confidence interval 0.10-0.76, P=.011). Conclusions: Hyperfractionated radiation therapy was associated with better executive function and worse growth but without accompanying change in health status, behavior, or quality of life.

  6. Long-Term Monitoring of Waterborne Pathogens and Microbial Source Tracking Markers in Paired Agricultural Watersheds under Controlled and Conventional Tile Drainage Management

    PubMed Central

    Wilkes, Graham; Brassard, Julie; Edge, Thomas A.; Gannon, Victor; Gottschall, Natalie; Jokinen, Cassandra C.; Jones, Tineke H.; Khan, Izhar U. H.; Marti, Romain; Sunohara, Mark D.; Topp, Edward

    2014-01-01

    Surface waters from paired agricultural watersheds under controlled tile drainage (CTD) and uncontrolled tile drainage (UCTD) were monitored over 7 years in order to determine if there was an effect of CTD (imposed during the growing season) on occurrences and loadings of bacterial and viral pathogens, coliphages, and microbial source tracking markers. There were significantly lower occurrences of human, ruminant, and livestock (ruminant plus pig) Bacteroidales markers in the CTD watershed in relation to the UCTD watershed. As for pathogens, there were significantly lower occurrences of Salmonella spp. and Arcobacter spp. in the CTD watershed. There were no instances where there were significantly higher quantitative loadings of any microbial target in the CTD watershed, except for F-specific DNA (F-DNA) and F-RNA coliphages, perhaps as a result of fecal inputs from a hobby farm independent of the drainage practice treatments. There was lower loading of the ruminant marker in the CTD watershed in relation to the UCTD system, and results were significant at the level P = 0.06. The odds of Salmonella spp. occurring increased when a ruminant marker was present relative to when the ruminant marker was absent, yet for Arcobacter spp., the odds of this pathogen occurring significantly decreased when a ruminant marker was present relative to when the ruminant marker was absent (but increased when a wildlife marker was present relative to when the wildlife marker was absent). Interestingly, the odds of norovirus GII (associated with human and swine) occurring in water increased significantly when a ruminant marker was present relative to when a ruminant marker was absent. Overall, this study suggests that fecal pollution from tile-drained fields to stream could be reduced by CTD utilization. PMID:24727274

  7. Educational Mismatch between Graduates' Possessed Skills and Market Demands in Pakistan

    ERIC Educational Resources Information Center

    Uzair-ul-Hassan, Muhammad; Noreen, Zahida

    2013-01-01

    Educational mismatch in skills that graduates possess and market requires creates barriers for organizations as well as for job seekers. The study was conducted to find out the educational mismatch between graduates possessed skills and market demands. Convenient sampling was carried out and data were collected from 200 graduates of economics…

  8. On the Mismatch between Multicultural Education and Its Subjects in the Field

    ERIC Educational Resources Information Center

    Mizrachi, Nissim

    2012-01-01

    This article draws attention to the growing evidence of a mismatch between sociological categorization and actors' worlds of meaning as expressed in the classroom. The mismatch is especially blatant in cases where students from disadvantaged groups are introduced to what educators and theorists presume to be the liberating discourse of…

  9. Fast Kids, Slow Kids, Lazy Kids: Framing the Mismatch Problem in Mathematics Teachers' Conversations

    ERIC Educational Resources Information Center

    Horn, Ilana Seidel

    2007-01-01

    This article examines the social nature of teachers' conceptions by showing how teachers frame the "mismatch" of students' perceived abilities and the intended school curriculum through conversational category systems. This study compares the conversations of 2 groups of high school mathematics teachers addressing the Mismatch Problem when…

  10. Novel DNA mismatch repair activity involving YB-1 in human mitochondria

    PubMed Central

    de Souza-Pinto, Nadja C.; Mason, Penelope A.; Hashiguchi, Kazunari; Weissman, Lior; Tian, Jingyan; Guay, David; Lebel, Michel; Stevnsner, Tinna V.; Rasmussen, Lene Juel; Bohr, Vilhelm A.

    2009-01-01

    Maintenance of the mitochondrial genome (mtDNA) is essential for proper cellular function. The accumulation of damage and mutations in the mtDNA leads to diseases, cancer, and aging. Mammalian mitochondria have proficient base excision repair, but the existence of other DNA repair pathways is still unclear. Deficiencies in DNA mismatch repair (MMR), which corrects base mismatches and small loops, are associated with DNA microsatellite instability, accumulation of mutations, and cancer. MMR proteins have been identified in yeast and coral mitochondria; however, MMR proteins and function have not yet been detected in human mitochondria. Here we show that human mitochondria have a robust mismatch-repair activity, which is distinct from nuclear MMR. Key nuclear MMR factors were not detected in mitochondria, and similar mismatch-binding activity was observed in mitochondrial extracts from cells lacking MSH2, suggesting distinctive pathways for nuclear and mitochondrial MMR. We identified the repair factor YB-1 as a key candidate for a mitochondrial mismatch-binding protein. This protein localizes to mitochondria in human cells, and contributes significantly to the mismatch-binding and mismatch-repair activity detected in HeLa mitochondrial extracts, which are significantly decreased when the intracellular levels of YB-1 are diminished. Moreover, YB-1 depletion in cells increases mitochondrial DNA mutagenesis. Our results show that human mitochondria contain a functional MMR repair pathway in which YB-1 participates, likely in the mismatch binding and recognition steps. PMID:19272840

  11. T cell-depleted partial matched unrelated donor transplant for advanced myeloid malignancy: KIR ligand mismatch and outcome.

    PubMed

    Weisdorf, Daniel; Cooley, Sarah; Devine, Steven; Fehniger, Todd A; DiPersio, John; Anasetti, Claudio; Waller, Edmund K; Porter, David; Farag, Sherif; Drobyski, William; Defor, Todd; Haagenson, Michael; Curtsinger, Julie; Miller, Jeffrey

    2012-06-01

    To evaluate the applicability of high-dose conditioning, CD34 selection, and enhanced natural killer (NK) cell alloreactivity reported as promising after haploidentical transplantation, we tested the same strategy for patients with advanced/high-risk myeloid leukemia lacking either related or well-matched unrelated donors (URD). In a prospective multicenter clinical trial using pretransplantation conditioning of thiotepa (5 mg/kg/day × 2), fludarabine (40 mg/mg/M(2)/day × 5), and total body radiation (800 cGy) plus thymoglobulin (2.5 mg/kg/day × 2), as well as a CD34 selected filgrastim stimulated peripheral blood graft from a partial matched URD, we treated 24 patients. The patients (median age 40 [range: 22-61]) were mismatched at 1-3 of 10 HLA loci with their donors; all were mismatched at HLA-C. Thirty-seven percent were ethnic or racial minorities. Twenty-one of 24 engrafted promptly with 1 primary graft failure and 2 early deaths. The cumulative incidence of grade II-IV acute graft-versus-host disease (aGVHD) (34%, 95% confidence interval [CI], 14-54%), chronic GVHD (20%, 95% CI, 2%-38%), and relapse (26%, 95% CI, 8%-84%) were unaffected by KIR ligand donor:recipient mismatch (n = 5) versus KIR ligand match (n = 19). Only 3 (12%) had grade III-IV GVHD. Nonrelapse occurred in 17% (95% CI, 30%-31%) by 100 days and in 35% (95% CI, 15%-55%) by 1 year. Two-year survival and leukemia-free survival were each 40% (95% CI, 21%-59%) and was similar in KIR ligand matched or mismatched patients. Infections, mostly in the first 2 months, were frequent, and were the cause of death in 5 patients (35% of deaths). T cell recovery and NK cell proliferation and functional maturation were not altered by KIR ligand match or mismatch status. For these high-risk patients, this high intensity regimen and T depleted approach yielded satisfactory outcomes, but logistical difficulties in arranging URD grafts for patients with high-risk, unstable leukemia limited accrual

  12. Conventional magnetic superconductors

    DOE PAGESBeta

    Wolowiec, C. T.; White, B. D.; Maple, M. B.

    2015-07-01

    We discuss several classes of conventional magnetic superconductors including the ternary rhodium borides and molybdenum chalcogenides (or Chevrel phases), and the quaternary nickel-borocarbides. These materials exhibit some exotic phenomena related to the interplay between superconductivity and long-range magnetic order including: the coexistence of superconductivity and antiferromagnetic order; reentrant and double reentrant superconductivity, magnetic field induced superconductivity, and the formation of a sinusoidally-modulated magnetic state that coexists with superconductivity. We introduce the article with a discussion of the binary and pseudobinary superconducting materials containing magnetic impurities which at best exhibit short-range “glassy” magnetic order. Early experiments on these materials led tomore » the idea of a magnetic exchange interaction between the localized spins of magnetic impurity ions and the spins of the conduction electrons which plays an important role in understanding conventional magnetic superconductors. Furthermore, these advances provide a natural foundation for investigating unconventional superconductivity in heavy-fermion compounds, cuprates, and other classes of materials in which superconductivity coexists with, or is in proximity to, a magnetically-ordered phase.« less

  13. Conventional magnetic superconductors

    SciTech Connect

    Wolowiec, C. T.; White, B. D.; Maple, M. B.

    2015-07-01

    We discuss several classes of conventional magnetic superconductors including the ternary rhodium borides and molybdenum chalcogenides (or Chevrel phases), and the quaternary nickel-borocarbides. These materials exhibit some exotic phenomena related to the interplay between superconductivity and long-range magnetic order including: the coexistence of superconductivity and antiferromagnetic order; reentrant and double reentrant superconductivity, magnetic field induced superconductivity, and the formation of a sinusoidally-modulated magnetic state that coexists with superconductivity. We introduce the article with a discussion of the binary and pseudobinary superconducting materials containing magnetic impurities which at best exhibit short-range “glassy” magnetic order. Early experiments on these materials led to the idea of a magnetic exchange interaction between the localized spins of magnetic impurity ions and the spins of the conduction electrons which plays an important role in understanding conventional magnetic superconductors. Furthermore, these advances provide a natural foundation for investigating unconventional superconductivity in heavy-fermion compounds, cuprates, and other classes of materials in which superconductivity coexists with, or is in proximity to, a magnetically-ordered phase.

  14. Electrochemical Investigation of Interaction between a Bifunctional Probe and GG Mismatch Duplex.

    PubMed

    Li, Jiao; He, Hanping; Peng, Xiaoqian; Huang, Min; Zhang, Xiuhua; Wang, Shengfu

    2015-01-01

    A bifunctional probe (FecNC), containing a recognition part and an electrochemical active center, was applied to electrochemical detection of GG mismatch duplexes. The preparation of gold electrodes modified by mismatch and complementatry duplexes was characterized by electrochemical impedance spectroscopy (EIS) and optimized for better detection in terms of self-assembly time, hybridization time, and incubation time. The interaction between FecNC and DNA duplexes modified on the surface of a gold electrode was explored by square wave voltammetry (SWV) and EIS. The results showed that the DNA duplexes with GG mismatch on the surface of a gold electrode was easily detected by the largest electrochemical signal of the bifunctional probe because of its selective binding to GG mismatches. The bifunctional probe could offer a simple, effective electrochemical detection of GG mismatches, and theoretical bases for development of electrochemical biosensors. Further, the method would be favorable for diagnosis of genetic diseases. PMID:26165289

  15. Compensation of IQ mismatch in optical PDM-OFDM coherent receivers

    NASA Astrophysics Data System (ADS)

    Chung, Hwan Seok; Chang, Sun Hyok; Kim, Kwangjoon

    2010-10-01

    The performance enhancements based on Gram-Schmidt orthogonalization procedure (GSOP) for compensating IQ mismatch in coherent optical orthogonal frequency division multiplexing (CO-OFDM) systems are investigated. We analytically explore IQ mismatch in optical OFDM systems and investigate the impacts of phase and amplitude IQ mismatch on required optical signal-to-noise ratio (OSNR) for the different values of data mapping and polarization multiplexing. The impacts of analog-to-digital converter (ADC) resolution and the number of samples in GSOP are also evaluated. The results show that the GSOP operation efficiently compensate IQ mismatch induced performance degradations regardless of the amount of IQ phase mismatch, density of data mapping, and polarization multiplexing.

  16. DNA Mismatch Repair and Oxidative DNA Damage: Implications for Cancer Biology and Treatment

    PubMed Central

    Bridge, Gemma; Rashid, Sukaina; Martin, Sarah A.

    2014-01-01

    Many components of the cell, including lipids, proteins and both nuclear and mitochondrial DNA, are vulnerable to deleterious modifications caused by reactive oxygen species. If not repaired, oxidative DNA damage can lead to disease-causing mutations, such as in cancer. Base excision repair and nucleotide excision repair are the two DNA repair pathways believed to orchestrate the removal of oxidative lesions. However, recent findings suggest that the mismatch repair pathway may also be important for the response to oxidative DNA damage. This is particularly relevant in cancer where mismatch repair genes are frequently mutated or epigenetically silenced. In this review we explore how the regulation of oxidative DNA damage by mismatch repair proteins may impact on carcinogenesis. We discuss recent studies that identify potential new treatments for mismatch repair deficient tumours, which exploit this non-canonical role of mismatch repair using synthetic lethal targeting. PMID:25099886

  17. Wavefront control algorithms and analysis for a dense adaptive optics system

    SciTech Connect

    Milman, M.; Fijany, A.; Redding, D.

    1994-12-31

    This paper presents the development and analysis of a wavefront control strategy for the Space Laser Electric Energy (SELENE) power being system. SELENE represents a substantial departure from most conventional adaptive optics systems in that the deformable element is the segmented primary mirror and the signal that is fed back includes both the local wavefront tilt and the relative edge mismatch between adjacent segments. The major challenge in designing the wavefront control system is the large number of subapertures that must be commanded. A fast and near optimal algorithm based on the local slope and edge measurements is defined for this system.

  18. Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.

    PubMed Central

    Kwok, S; Kellogg, D E; McKinney, N; Spasic, D; Goda, L; Levenson, C; Sninsky, J J

    1990-01-01

    We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C:C mismatches reduced overall PCR product yield about 100-fold, A:A mismatches about 20-fold. All other 3'-terminal mismatches were efficiently amplified, although the G:G mismatches appeared to be more sensitive to sequence context and dNTP concentrations than other mismatches. It should be noted that mismatches of T with either G, C, or T had a minimal effect on PCR product yield. Double mismatches within the last four bases of a primer-template duplex where one of the mismatches is at the 3' terminal nucleotide, in general, reduced PCR product yield dramatically. The presence of a mismatched T at the 3'-terminus, however, allowed significant amplification even when coupled with an adjacent mismatch. Furthermore, even two mismatched Ts at the 3'-terminus allowed efficient amplification. Images PMID:2179874

  19. Effects of refractive index mismatch in optical CT imaging of polymer gel dosimeters

    SciTech Connect

    Manjappa, Rakesh; Makki S, Sharath; Kanhirodan, Rajan; Kumar, Rajesh

    2015-02-15

    Purpose: Proposing an image reconstruction technique, algebraic reconstruction technique-refraction correction (ART-rc). The proposed method takes care of refractive index mismatches present in gel dosimeter scanner at the boundary, and also corrects for the interior ray refraction. Polymer gel dosimeters with high dose regions have higher refractive index and optical density compared to the background medium, these changes in refractive index at high dose results in interior ray bending. Methods: The inclusion of the effects of refraction is an important step in reconstruction of optical density in gel dosimeters. The proposed ray tracing algorithm models the interior multiple refraction at the inhomogeneities. Jacob’s ray tracing algorithm has been modified to calculate the pathlengths of the ray that traverses through the higher dose regions. The algorithm computes the length of the ray in each pixel along its path and is used as the weight matrix. Algebraic reconstruction technique and pixel based reconstruction algorithms are used for solving the reconstruction problem. The proposed method is tested with numerical phantoms for various noise levels. The experimental dosimetric results are also presented. Results: The results show that the proposed scheme ART-rc is able to reconstruct optical density inside the dosimeter better than the results obtained using filtered backprojection and conventional algebraic reconstruction approaches. The quantitative improvement using ART-rc is evaluated using gamma-index. The refraction errors due to regions of different refractive indices are discussed. The effects of modeling of interior refraction in the dose region are presented. Conclusions: The errors propagated due to multiple refraction effects have been modeled and the improvements in reconstruction using proposed model is presented. The refractive index of the dosimeter has a mismatch with the surrounding medium (for dry air or water scanning). The algorithm

  20. How Tolerant are Membrane Simulations with Mismatch in Area per Lipid between Leaflets?

    PubMed

    Park, Soohyung; Beaven, Andrew H; Klauda, Jeffery B; Im, Wonpil

    2015-07-14

    Difficulties in estimating the correct number of lipids in each leaflet of complex bilayer membrane simulation systems make it inevitable to introduce a mismatch in lipid packing (i.e., area per lipid) and thus alter the lateral pressure of each leaflet. To investigate potential impacts of such mismatch on simulation results, we performed molecular dynamics simulations of saturated and monounsaturated lipid bilayers with and without gramicidin A or WALP23 at various mismatches by adjusting the number of lipids in the lower leaflet from no mismatch to a 25% reduction compared to that in the upper leaflet. All simulations were stable under the constant pressure barostat, but the mismatch induces asymmetric lipid packing between the leaflets, so that the upper leaflet becomes more ordered, and the lower leaflet becomes less ordered. The mismatch impacts on various bilayer properties are mild up to 5-10% mismatch, and bilayers with fully saturated chains appear to be more prone to these impacts than those with unsaturated tails. The nonvanishing leaflet surface tensions and the free energy derivatives with respect to the bilayer curvature indicate that the bilayer would be energetically unstable in the presence of mismatch. We propose a quantitative criterion for allowable mismatch based on the energetics derived from a continuum elastic model, which grows as a square root of the number of the lipids in the system. On the basis of this criterion, we infer that the area per lipid mismatch up to 5% would be tolerable in various membrane simulations of reasonable all-atom system sizes (40-160 lipids per leaflet). PMID:26575780

  1. Pseudotumour incidence, cobalt levels and clinical outcome after large head metal-on-metal and conventional metal-on-polyethylene total hip arthroplasty: mid-term results of a randomised controlled trial.

    PubMed

    van der Veen, H C; Reininga, I H F; Zijlstra, W P; Boomsma, M F; Bulstra, S K; van Raay, J J A M

    2015-11-01

    We compared the incidence of pseudotumours after large head metal-on-metal (MoM) total hip arthroplasty (THA) with that after conventional metal-on-polyethylene (MoP) THA and assessed the predisposing factors to pseudotumour formation. From a previous randomised controlled trial which compared large head (38 mm to 60 mm) cementless MoM THA with conventional head (28 mm) cementless MoP THA, 93 patients (96 THAs: 41 MoM (21 males, 20 females, mean age of 64 years, standard deviation (sd) 4) and 55 MoP (25 males, 30 females, mean age of 65 years, sd 5) were recruited after a mean follow-up of 50 months (36 to 64). The incidence of pseudotumours, measured using a standardised CT protocol was 22 (53.7%) after MoM THA and 12 (21.8%) after MoP THA. Women with a MoM THA were more likely to develop a pseudotumour than those with a MoP THA (15 vs 7, odds ratio (OR) = 13.4, p < 0.001). There was a similar incidence of pseudotumours in men with MoM THAs and those with MoP THAs (7 vs 5, OR = 2.1, p = 0.30). Elevated cobalt levels (≥ 5 microgram/L) were only associated with pseudotumours in women with a MoM THA. There was no difference in mean Oxford and Harris hip scores between patients with a pseudotumour and those without. Contrary to popular belief, pseudotumours occur frequently around MoP THAs. Women with a MoM THA and an elevated cobalt level are at greatest risk. In this study, pseudotumours had no effect on the functional outcome after either large head MoM or conventional MoP THA. PMID:26530649

  2. Increasing the sensitivity and single-base mismatch selectivity of the molecular beacon using graphene oxide as the "nanoquencher".

    PubMed

    Lu, Chun-Hua; Li, Juan; Liu, Jing-Jing; Yang, Huang-Hao; Chen, Xi; Chen, Guo-Nan

    2010-04-26

    Here, we report a novel, highly sensitive, selective and economical molecular beacon using graphene oxide as the "nanoquencher". This novel molecular beacon system contains a hairpin-structured fluorophore-labeled oligonucleotide and a graphene oxide sheet. The strong interaction between hairpin-structured oligonucleotide and graphene oxide keep them in close proximity, facilitating the fluorescence quenching of the fluorophore by graphene oxide. In the presence of a complementary target DNA, the binding between hairpin-structured oligonucleotide and target DNA will disturb the interaction between hairpin-structured oligonucleotide and graphene oxide, and release the oligonucleotide from graphene oxide, resulting in restoration of fluorophore fluorescence. In the present study, we show that this novel graphene oxide quenched molecular beacon can be used to detect target DNA with higher sensitivity and single-base mismatch selectivity compared to the conventional molecular beacon. PMID:20301144

  3. Analysis of the effects of mismatched errors on coherent beam combining based on a self-imaging waveguide

    NASA Astrophysics Data System (ADS)

    Tao, R.; Wang, X.; Zhou, Pu; Si, Lei

    2016-01-01

    A theoretical model of coherent beam combining (CBC) based on a self-imaging waveguide (SIW) is built and the effects of mismatched errors on SIW-based CBC are simulated and analysed numerically. With the combination of the theoretical model and the finite difference beam propagation method, two main categories of errors, assembly and nonassembly errors, are numerically studied to investigate their effect on the beam quality by using the M2 factor. The optimisation of the SIW and error control principle of the system is briefly discussed. The generalised methodology offers a good reference for investigating waveguide-based high-power coherent combining of fibre lasers in a comprehensive way.

  4. Biodiesel from conventional feedstocks.

    PubMed

    Du, Wei; Liu, De-Hua

    2012-01-01

    At present, traditional fossil fuels are used predominantly in China, presenting the country with challenges that include sustainable energy supply, energy efficiency improvement, and reduction of greenhouse gas emissions. In 2007, China issued The Strategic Plan of the Mid-and-Long Term Development of Renewable Energy, which aims to increase the share of clean energy in the country's energy consumption to 15% by 2020 from only 7.5% in 2005. Biodiesel, an important renewable fuel with significant advantages over fossil diesel, has attracted great attention in the USA and European countries. However, biodiesel is still in its infancy in China, although its future is promising. This chapter reviews biodiesel production from conventional feedstocks in the country, including feedstock supply and state of the art technologies for the transesterification reaction through which biodiesel is made, particularly the enzymatic catalytic process developed by Chinese scientists. Finally, the constraints and perspectives for China's biodiesel development are highlighted. PMID:22085921

  5. Conventional therapies for psoriasis.

    PubMed

    Rebora, A

    2007-01-01

    Conventional treatments of psoriasis include topical and systemic drugs. For sake of brevity, the presentation will deal only with systemic therapy. Three drugs are presently available in Italy: methotrexate, acitretin and cyclosporin A. Their efficacy is almost identical, all of them achieving PASI 75 in about 60% of cases in 12 weeks The indications (which, in Italy, do not include psoriasis for methotrexate), the contraindications, the interactions, the adverse effects and the precautions in their use will be discussed. Methotrexate side effects account for more than 10% of cases and include nausea and vomiting and chiefly increase of blood levels of liver enzymes. Acitretin side effects are numerous and varied, the most severe being increase of liver enzymes and blood lipids, renal impairment, and teratogenicity. Cyclosporin side effects are chiefly hypertension and renal failure. The Author concludes that cyclosporin is the drug with the best efficacy/side effect ratio, though it should be used in selected cases. PMID:17828351

  6. Epitaxial Growth of Lattice-Mismatched Core-Shell TiO2 @MoS2 for Enhanced Lithium-Ion Storage.

    PubMed

    Dai, Rui; Zhang, Anqi; Pan, Zhichang; Al-Enizi, Abdullah M; Elzatahry, Ahmed A; Hu, Linfeng; Zheng, Gengfeng

    2016-05-01

    Core-shell structured nanohybrids are currently of significant interest due to their synergetic properties and enhanced performances. However, the restriction of lattice mismatch remains a severe obstacle for heterogrowth of various core-shells with two distinct crystal structures. Herein, a controlled synthesis of lattice-mismatched core-shell TiO2 @MoS2 nano-onion heterostructures is successfully developed, using unilamellar Ti0.87 O2 nanosheets as the starting material and the subsequent epitaxial growth of MoS2 on TiO2 . The formation of these core-shell nano-onions is attributed to an amorphous layer-induced heterogrowth mechanism. The number of MoS2 layers can be well tuned from few to over ten layers, enabling layer-dependent synergistic effects. The core-shell TiO2 @MoS2 nano-onion heterostructures exhibit significantly enhanced energy storage performance as lithium-ion battery anodes. The approach has also been extended to other lattice-mismatched systems such as TiO2 @MoSe2 , thus suggesting a new strategy for the growth of well-designed lattice-mismatched core-shell structures. PMID:27062267

  7. Effectiveness of conventional versus virtual reality based vestibular rehabilitation in the treatment of dizziness, gait and balance impairment in adults with unilateral peripheral vestibular loss: a randomised controlled trial

    PubMed Central

    2012-01-01

    Background Unilateral peripheral vestibular loss results in gait and balance impairment, dizziness and oscillopsia. Vestibular rehabilitation benefits patients but optimal treatment remains unkown. Virtual reality is an emerging tool in rehabilitation and provides opportunities to improve both outcomes and patient satisfaction with treatment. The Nintendo Wii Fit Plus® (NWFP) is a low cost virtual reality system that challenges balance and provides visual and auditory feedback. It may augment the motor learning that is required to improve balance and gait, but no trials to date have investigated efficacy. Methods/Design In a single (assessor) blind, two centre randomised controlled superiority trial, 80 patients with unilateral peripheral vestibular loss will be randomised to either conventional or virtual reality based (NWFP) vestibular rehabilitation for 6 weeks. The primary outcome measure is gait speed (measured with three dimensional gait analysis). Secondary outcomes include computerised posturography, dynamic visual acuity, and validated questionnaires on dizziness, confidence and anxiety/depression. Outcome will be assessed post treatment (8 weeks) and at 6 months. Discussion Advances in the gaming industry have allowed mass production of highly sophisticated low cost virtual reality systems that incorporate technology previously not accessible to most therapists and patients. Importantly, they are not confined to rehabilitation departments, can be used at home and provide an accurate record of adherence to exercise. The benefits of providing augmented feedback, increasing intensity of exercise and accurately measuring adherence may improve conventional vestibular rehabilitation but efficacy must first be demonstrated. Trial registration Clinical trials.gov identifier: NCT01442623 PMID:22449224

  8. Deficient mismatch repair: Read all about it (Review)

    PubMed Central

    RICHMAN, SUSAN

    2015-01-01

    Defects in the DNA mismatch repair (MMR) proteins, result in a phenotype called microsatellite instability (MSI), occurring in up to 15% of sporadic colorectal cancers. Approximately one quarter of colon cancers with deficient MMR (dMMR) develop as a result of an inherited predisposition syndrome, Lynch syndrome (formerly known as HNPCC). It is essential to identify patients who potentially have Lynch syndrome, as not only they, but also family members, may require screening and monitoring. Diagnostic criteria have been developed, based primarily on Western populations, and several methodologies are available to identify dMMR tumours, including immunohistochemistry and microsatellite testing. These criteria have provided evidence supporting the introduction of reflex testing. Yet, it is becoming increasingly clear that tests have a limited sensitivity and specificity and may yet be superseded by next generation sequencing. In this review, the limitations of diagnostic criteria are discussed, and current and emerging screening technologies explained. There is now useful evidence supporting the prognostic and predictive value of dMMR status in colorectal tumours, but much less is known about their value in extracolonic tumours, that may also feature in Lynch syndrome. This review assesses current literature relating to dMMR in endometrial, ovarian, gastric and melanoma cancers, which it would seem, may benefit from large-scale clinical trials in order to further close the gap in knowledge between colorectal and extracolonic tumours. PMID:26315971

  9. A frontal attention mechanism in the visual mismatch negativity

    PubMed Central

    Hedge, Craig; Stothart, George; Todd Jones, Jenna; Rojas Frías, Priscila; Magee, Kristopher Lundy; Brooks, Jonathan C.W.

    2015-01-01

    Automatic detection of environmental change is a core component of attention. The mismatch negativity (MMN), an electrophysiological marker of this mechanism, has been studied prominently in the auditory domain, with cortical generators identified in temporal and frontal regions. Here, we combined electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) to assess whether the underlying frontal regions associated with auditory change detection also play a role in visual change detection. Twenty healthy young adults completed a visual MMN task in separate EEG and fMRI sessions. Region of interest analyses were conducted on left and right middle frontal (MFG) and inferior frontal (IFG) gyri, i.e., the frontal areas identified as potential auditory MMN generators. A significant increase in activation was observed in the left IFG and MFG in response to blocks containing deviant stimuli. These findings suggest that a frontal mechanism is involved in the detection of change in the visual MMN. Our results support the notion that frontal mechanisms underlie attention switching, as measured via MMN, across multiple modalities. PMID:26183650

  10. A frontal attention mechanism in the visual mismatch negativity.

    PubMed

    Hedge, Craig; Stothart, George; Todd Jones, Jenna; Rojas Frías, Priscila; Magee, Kristopher Lundy; Brooks, Jonathan C W

    2015-10-15

    Automatic detection of environmental change is a core component of attention. The mismatch negativity (MMN), an electrophysiological marker of this mechanism, has been studied prominently in the auditory domain, with cortical generators identified in temporal and frontal regions. Here, we combined electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) to assess whether the underlying frontal regions associated with auditory change detection also play a role in visual change detection. Twenty healthy young adults completed a visual MMN task in separate EEG and fMRI sessions. Region of interest analyses were conducted on left and right middle frontal (MFG) and inferior frontal (IFG) gyri, i.e., the frontal areas identified as potential auditory MMN generators. A significant increase in activation was observed in the left IFG and MFG in response to blocks containing deviant stimuli. These findings suggest that a frontal mechanism is involved in the detection of change in the visual MMN. Our results support the notion that frontal mechanisms underlie attention switching, as measured via MMN, across multiple modalities. PMID:26183650

  11. MCM9 Is Required for Mammalian DNA Mismatch Repair.

    PubMed

    Traver, Sabine; Coulombe, Philippe; Peiffer, Isabelle; Hutchins, James R A; Kitzmann, Magali; Latreille, Daniel; Méchali, Marcel

    2015-09-01

    DNA mismatch repair (MMR) is an evolutionarily conserved process that corrects DNA polymerase errors during replication to maintain genomic integrity. In E. coli, the DNA helicase UvrD is implicated in MMR, yet an analogous helicase activity has not been identified in eukaryotes. Here, we show that mammalian MCM9, a protein involved in replication and homologous recombination, forms a complex with MMR initiation proteins (MSH2, MSH3, MLH1, PMS1, and the clamp loader RFC) and is essential for MMR. Mcm9-/- cells display microsatellite instability and MMR deficiency. The MCM9 complex has a helicase activity that is required for efficient MMR since wild-type but not helicase-dead MCM9 restores MMR activity in Mcm9-/- cells. Moreover, MCM9 loading onto chromatin is MSH2-dependent, and in turn MCM9 stimulates the recruitment of MLH1 to chromatin. Our results reveal a role for MCM9 and its helicase activity in mammalian MMR. PMID:26300262

  12. Responses of prefrontal multisensory neurons to mismatching faces and vocalizations.

    PubMed

    Diehl, Maria M; Romanski, Lizabeth M

    2014-08-20

    Social communication relies on the integration of auditory and visual information, which are present in faces and vocalizations. Evidence suggests that the integration of information from multiple sources enhances perception compared with the processing of a unimodal stimulus. Our previous studies demonstrated that single neurons in the ventrolateral prefrontal cortex (VLPFC) of the rhesus monkey (Macaca mulatta) respond to and integrate conspecific vocalizations and their accompanying facial gestures. We were therefore interested in how VLPFC neurons respond differentially to matching (congruent) and mismatching (incongruent) faces and vocalizations. We recorded VLPFC neurons during the presentation of movies with congruent or incongruent species-specific facial gestures and vocalizations as well as their unimodal components. Recordings showed that while many VLPFC units are multisensory and respond to faces, vocalizations, or their combination, a subset of neurons showed a significant change in neuronal activity in response to incongruent versus congruent vocalization movies. Among these neurons, we typically observed incongruent suppression during the early stimulus period and incongruent enhancement during the late stimulus period. Incongruent-responsive VLPFC neurons were both bimodal and nonlinear multisensory, fostering their ability to respond to changes in either modality of a face-vocalization stimulus. These results demonstrate that ventral prefrontal neurons respond to changes in either modality of an audiovisual stimulus, which is important in identity processing and for the integration of multisensory communication information. PMID:25143605

  13. Modeling of integrated environmental control systems for coal-fired power plants: Conventional froth flotation for the IEC coal cleaning plant model. Quarterly progress report, [October 1, 1988--December 31, 1988

    SciTech Connect

    Rubin, E.S.

    1989-01-01

    This report describes the addition of a conventional froth flotation circuit into the FORTRAN coal cleaning module of the Integrated Environmental Control (IEC) model. The purpose of this modification is to include froth flotation as an option to clean the coal fines. The current model has three beneficiation: levels (2, 3, and 4) in which different streams are washed by specific gravity equipment. Level 2 washes only the coarse stream. Level 3 washes the coarse and medium streams. Level 4 washes the coarse, medium, and fine streams. This modification adds a fifth level, which uses specific gravity equipment to wash the coarse and medium streams and froth flotation equipment for the fine stream. The specific size fractions in each stream are specified by the model user. As before, the model optimizes the yield of each circuit in order to achieve a target coal quality for the cleaned coal product.

  14. A 12-bit 3.7-Msample/s Pipelined A/D Converter Based on the Novel Capacitor Mismatch Calibration Technique

    NASA Astrophysics Data System (ADS)

    Wang, Shuaiqi; Li, Fule; Inoue, Yasuaki

    This paper proposes a 12-bit 3.7-MS/s pipelined A/D Converter based on the novel capacitor mismatch calibration technique. The conventional stage is improved to an algorithmic circuit involving charge summing, capacitors' exchange and charge redistribution, simply through introducing some extra switches into the analog circuit. This proposed ADC obtains the linearity beyond the accuracy of the capacitor match and verifies the validity of reducing the nonlinear error from the capacitor mismatch to the second order without additional power dissipation through the novel capacitor mismatch calibration technique. It is processed in 0.5μm CMOS technology. The transistor-level simulation results show that 72.6dB SNDR, 78.5dB SFDR are obtained for a 2V Vpp 159.144kHz sine input sampled at 3.7MS/s. The whole power dissipation of this ADC is 33.4mW at the power supply of 5V.

  15. Ventromedial prefrontal cortex drives hippocampal theta oscillations induced by mismatch computations.

    PubMed

    Garrido, Marta I; Barnes, Gareth R; Kumaran, Dharshan; Maguire, Eleanor A; Dolan, Raymond J

    2015-10-15

    Detecting environmental change is fundamental for adaptive behavior in an uncertain world. Previous work indicates the hippocampus supports the generation of novelty signals via implementation of a match-mismatch detector that signals when an incoming sensory input violates expectations based on past experience. While existing work has emphasized the particular contribution of the hippocampus, here we ask which other brain structures also contribute to match-mismatch detection. Furthermore, we leverage the fine-grained temporal resolution of magnetoencephalography (MEG) to investigate whether mismatch computations are spectrally confined to the theta range, based on the prominence of this range of oscillations in models of hippocampal function. By recording MEG activity while human subjects perform a task that incorporates conditions of match-mismatch novelty we show that mismatch signals are confined to the theta band and are expressed in both the hippocampus and ventromedial prefrontal cortex (vmPFC). Effective connectivity analyses (dynamic causal modeling) show that the hippocampus and vmPFC work as a functional circuit during mismatch detection. Surprisingly, our results suggest that the vmPFC drives the hippocampus during the generation and processing of mismatch signals. Our findings provide new evidence that the hippocampal-vmPFC circuit is engaged during novelty processing, which has implications for emerging theories regarding the role of vmPFC in memory. PMID:26187453

  16. Snowshoe hares display limited phenotypic plasticity to mismatch in seasonal camouflage

    PubMed Central

    Zimova, Marketa; Mills, L. Scott; Lukacs, Paul M.; Mitchell, Michael S.

    2014-01-01

    As duration of snow cover decreases owing to climate change, species undergoing seasonal colour moults can become colour mismatched with their background. The immediate adaptive solution to this mismatch is phenotypic plasticity, either in phenology of seasonal colour moults or in behaviours that reduce mismatch or its consequences. We observed nearly 200 snowshoe hares across a wide range of snow conditions and two study sites in Montana, USA, and found minimal plasticity in response to mismatch between coat colour and background. We found that moult phenology varied between study sites, likely due to differences in photoperiod and climate, but was largely fixed within study sites with only minimal plasticity to snow conditions during the spring white-to-brown moult. We also found no evidence that hares modify their behaviour in response to colour mismatch. Hiding and fleeing behaviours and resting spot preference of hares were more affected by variables related to season, site and concealment by vegetation, than by colour mismatch. We conclude that plasticity in moult phenology and behaviours in snowshoe hares is insufficient for adaptation to camouflage mismatch, suggesting that any future adaptation to climate change will require natural selection on moult phenology or behaviour. PMID:24619446

  17. Ventromedial prefrontal cortex drives hippocampal theta oscillations induced by mismatch computations

    PubMed Central

    Garrido, Marta I.; Barnes, Gareth R.; Kumaran, Dharshan; Maguire, Eleanor A.; Dolan, Raymond J.

    2015-01-01

    Detecting environmental change is fundamental for adaptive behavior in an uncertain world. Previous work indicates the hippocampus supports the generation of novelty signals via implementation of a match–mismatch detector that signals when an incoming sensory input violates expectations based on past experience. While existing work has emphasized the particular contribution of the hippocampus, here we ask which other brain structures also contribute to match–mismatch detection. Furthermore, we leverage the fine-grained temporal resolution of magnetoencephalography (MEG) to investigate whether mismatch computations are spectrally confined to the theta range, based on the prominence of this range of oscillations in models of hippocampal function. By recording MEG activity while human subjects perform a task that incorporates conditions of match–mismatch novelty we show that mismatch signals are confined to the theta band and are expressed in both the hippocampus and ventromedial prefrontal cortex (vmPFC). Effective connectivity analyses (dynamic causal modeling) show that the hippocampus and vmPFC work as a functional circuit during mismatch detection. Surprisingly, our results suggest that the vmPFC drives the hippocampus during the generation and processing of mismatch signals. Our findings provide new evidence that the hippocampal–vmPFC circuit is engaged during novelty processing, which has implications for emerging theories regarding the role of vmPFC in memory. PMID:26187453

  18. Snowshoe hares display limited phenotypic plasticity to mismatch in seasonal camouflage

    USGS Publications Warehouse

    Zimova, Marketa; Mills, L. Scott; Lukacs, Paul M.; Mitchell, Michael S.

    2014-01-01

    As duration of snow cover decreases owing to climate change, species undergoing seasonal colour moults can become colour mismatched with their background. The immediate adaptive solution to this mismatch is phenotypic plasticity, either in phenology of seasonal colour moults or in behaviours that reduce mismatch or its consequences. We observed nearly 200 snowshoe hares across a wide range of snow conditions and two study sites in Montana, USA, and found minimal plasticity in response to mismatch between coat colour and background. We found that moult phenology varied between study sites, likely due to differences in photoperiod and climate, but was largely fixed within study sites with only minimal plasticity to snow conditions during the spring white-to-brown moult. We also found no evidence that hares modify their behaviour in response to colour mismatch. Hiding and fleeing behaviours and resting spot preference of hares were more affected by variables related to season, site and concealment by vegetation, than by colour mismatch. We conclude that plasticity in moult phenology and behaviours in snowshoe hares is insufficient for adaptation to camouflage mismatch, suggesting that any future adaptation to climate change will require natural selection on moult phenology or behaviour.

  19. Comparison of the Efficacy and Safety of a Pharmacokinetic Model-Based Dosing Scheme Versus a Conventional Fentanyl Dosing Regimen For Patient-Controlled Analgesia Immediately Following Robot-Assisted Laparoscopic Prostatectomy: A Randomized Clinical Trial.

    PubMed

    Jin, Seok-Joon; Lim, Hyeong-Seok; Kwon, Youn-Ju; Park, Se-Ung; Yi, Jung-Min; Chin, Ji-Hyun; Hwang, Jai-Hyun; Kim, Young-Kug

    2016-01-01

    Conventional, intravenous, patient-controlled analgesia, which is only administered by demand bolus without basal continuous infusion, is closely associated with inappropriate analgesia. Pharmacokinetic model-based dosing schemes can quantitatively describe the time course of drug effects and achieve optimal drug therapy. We compared the efficacy and safety of a conventional dosing regimen for intravenous patient-controlled analgesia that was administered by demand bolus without basal continuous infusion (group A) versus a pharmacokinetic model-based dosing scheme performed by decreasing the dosage of basal continuous infusion according to the model-based simulation used to achieve a targeted concentration (group B) following robot-assisted laparoscopic prostatectomy.In total, 70 patients were analyzed: 34 patients in group A and 36 patients in group B. The postoperative opioid requirements, pain scores assessed by the visual analog scale, and adverse events (eg, nausea, vomiting, pruritis, respiratory depression, desaturation, sedation, confusion, and urinary retention) were compared on admission to the postanesthesia care unit and at 0.5, 1, 4, 24, and 48 h after surgery between the 2 groups. All patients were kept for close observation in the postanesthesia care unit for 1 h, and then transferred to the general ward.The fentanyl requirements in the postanesthesia care unit for groups A and B were 110.0 ± 46.4 μg and 77.5 ± 35.3 μg, respectively. The pain scores assessed by visual analog scale at 0.5, 1, 4, and 24 h after surgery in group B were significantly lower than in group A (all P < 0.05). There were no differences in the adverse events between the 2 groups.We found that the pharmacokinetic model-based dosing scheme resulted in lower opioid requirements, lower pain scores, and no significant adverse events in the postanesthesia care unit following robot-assisted laparoscopic prostatectomy in comparison with conventional dosing

  20. A randomised controlled trial and cost-effectiveness analysis of high-frequency oscillatory ventilation against conventional artificial ventilation for adults with acute respiratory distress syndrome. The OSCAR (OSCillation in ARDS) study.

    PubMed Central

    Lall, Ranjit; Hamilton, Patrick; Young, Duncan; Hulme, Claire; Hall, Peter; Shah, Sanjoy; MacKenzie, Iain; Tunnicliffe, William; Rowan, Kathy; Cuthbertson, Brian; McCabe, Chris; Lamb, Sallie

    2015-01-01

    BACKGROUND Patients with the acute respiratory distress syndrome (ARDS) require artificial ventilation but this treatment may produce secondary lung damage. High-frequency oscillatory ventilation (HFOV) may reduce this damage. OBJECTIVES To determine the clinical benefit and cost-effectiveness of HFOV in patients with ARDS compared with standard mechanical ventilation. DESIGN A parallel, randomised, unblinded clinical trial. SETTING UK intensive care units. PARTICIPANTS Mechanically ventilated patients with a partial pressure of oxygen in arterial blood/fractional concentration of inspired oxygen (P : F) ratio of 26.7 kPa (200 mmHg) or less and an expected duration of ventilation of at least 2 days at recruitment. INTERVENTIONS Treatment arm HFOV using a Novalung R100(®) ventilator (Metran Co. Ltd, Saitama, Japan) ventilator until the start of weaning. Control arm Conventional mechanical ventilation using the devices available in the participating centres. MAIN OUTCOME MEASURES The primary clinical outcome was all-cause mortality at 30 days after randomisation. The primary health economic outcome was the cost per quality-adjusted life-year (QALY) gained. RESULTS One hundred and sixty-six of 398 patients (41.7%) randomised to the HFOV group and 163 of 397 patients (41.1%) randomised to the conventional mechanical ventilation group died within 30 days of randomisation (p = 0.85), for an absolute difference of 0.6% [95% confidence interval (CI) -6.1% to 7.5%]. After adjustment for study centre, sex, Acute Physiology and Chronic Health Evaluation II score, and the initial P : F ratio, the odds ratio for survival in the conventional ventilation group was 1.03 (95% CI 0.75 to 1.40; p = 0.87 logistic regression). Survival analysis showed no difference in the probability of survival up to 12 months after randomisation. The average QALY at 1 year in the HFOV group was 0.302 compared to 0.246. This gives an incremental cost-effectiveness ratio (ICER) for the cost to