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Sample records for copper ii sulfate

  1. Decomposition of Copper (II) Sulfate Pentahydrate: A Sequential Gravimetric Analysis.

    ERIC Educational Resources Information Center

    Harris, Arlo D.; Kalbus, Lee H.

    1979-01-01

    Describes an improved experiment of the thermal dehydration of copper (II) sulfate pentahydrate. The improvements described here are control of the temperature environment and a quantitative study of the decomposition reaction to a thermally stable oxide. Data will suffice to show sequential gravimetric analysis. (Author/SA)

  2. Spectroscopic study of copper(II) complexes with carboxymethyl dextran and dextran sulfate

    NASA Astrophysics Data System (ADS)

    Glišić, S.; Nikolić, G.; Cakić, M.; Trutić, N.

    2015-07-01

    The copper(II) ion complexes with carboxymethyl dextran (CMD) and dextran sulfate (DS) were studied by different methods. Content of copper was determined by atomic absorption spectroscopy. It was found that copper : ligand mole ratio (Cu : CMD) is 1 : 2, and Cu : DS is 1 : 1 by mole ratio method. Spectrophotometric parameters of synthesized compounds are characteristic for Cu(II) ion in octahedral ( O h ) coordination. Analyzing of FTIR spectra in ν(C=O) vibration region has showed that -COOH group acts as bidentate ligand, while the compounds of Cu(II) with DS copper ions are in the region of four oxygen atoms of two adjacent sulfo groups. The presence of crystalline water was determined by isotopic substitution of H2O molecules with D2O molecules. Comparison of spectra recorded at room (RT) and liquid nitrogen temperature (LNT) has enabled detection bands of water molecules libration indicating that they are coordinated complementing coordination sphere of Cu(II) ions to six. The complexes are of Cu(II) · (CMD)2 · (H2O)2 or Cu(II) · DS · (H2O)2 type. The similarities of the γ(C-H) range in a part of FTIR spectra indicate that there is no difference in the conformation of the 4 C 1 glucopyranose (Glc) unit CMD and DS synthesized Cu(II) complexes.

  3. Sulfate-bridged dimeric trinuclear copper(II)-pyrazolate complex with three different terminal ligands.

    PubMed

    Mezei, Gellert

    2016-08-01

    The reaction of CuSO4·5H2O, 4-chloro-pyrazole (4-Cl-pzH) and tri-ethyl-amine (Et3N) in di-methyl-formamide (DMF) produced crystals of di-aqua-hexa-kis-(μ-4-chloro-pyrazolato-κ(2) N:N')bis-(N,N-di-methyl-formamide)di-μ3-hydroxido-bis-(μ4-sulfato-κ(4) O:O':O'':O'')hexa-copper(II) N,N-di-methyl-formamide tetra-solvate dihydrate, [Cu3(OH)(SO4)(C3H2ClN2)3(C3H7NO)(H2O)]2·4C3H7NO·2H2O. The centrosymmetric dimeric molecule consists of two trinuclear copper-pyrazolate units bridged by two sulfate ions. The title compound provides the first example of a trinuclear copper-pyrazolate complex with three different terminal ligands on the Cu atoms, and also the first example of such complex with a strongly binding basal sulfate ion. Within each trinuclear unit, the Cu(II) atoms are bridged by μ-pyrazolate groups and a central μ3-OH group, and are coordinated by terminal sulfate, H2O and DMF ligands, respectively. Moreover, the sulfate O atoms coordinate at the apical position to the Cu atoms of the symmetry-related unit, providing square-pyramidal coordination geometry around each copper cation. The metal complex and solvent mol-ecules are involved in O-H⋯O hydrogen bonds, leading to a two-dimensional network parallel to (10-1). PMID:27536383

  4. Sulfate-bridged dimeric trinuclear copper(II)–pyrazolate complex with three different terminal ligands

    PubMed Central

    Mezei, Gellert

    2016-01-01

    The reaction of CuSO4·5H2O, 4-chloro­pyrazole (4-Cl-pzH) and tri­ethyl­amine (Et3N) in di­methyl­formamide (DMF) produced crystals of di­aqua­hexa­kis­(μ-4-chloro­pyrazolato-κ2 N:N′)bis­(N,N-di­methyl­formamide)di-μ3-hydroxido-bis­(μ4-sulfato-κ4 O:O′:O′′:O′′)hexa­copper(II) N,N-di­methyl­formamide tetra­solvate dihydrate, [Cu3(OH)(SO4)(C3H2ClN2)3(C3H7NO)(H2O)]2·4C3H7NO·2H2O. The centrosymmetric dimeric molecule consists of two trinuclear copper–pyrazolate units bridged by two sulfate ions. The title compound provides the first example of a trinuclear copper–pyrazolate complex with three different terminal ligands on the Cu atoms, and also the first example of such complex with a strongly binding basal sulfate ion. Within each trinuclear unit, the CuII atoms are bridged by μ-pyrazolate groups and a central μ3-OH group, and are coordinated by terminal sulfate, H2O and DMF ligands, respectively. Moreover, the sulfate O atoms coordinate at the apical position to the Cu atoms of the symmetry-related unit, providing square–pyramidal coordination geometry around each copper cation. The metal complex and solvent mol­ecules are involved in O—H⋯O hydrogen bonds, leading to a two-dimensional network parallel to (10-1). PMID:27536383

  5. Tris(2,2′-bipyridyl-κ2 N,N′)copper(II) sulfate 7.5-hydrate

    PubMed Central

    Xu, Feng; You, Wei; Huang, Wei

    2009-01-01

    The title compound, [Cu(C10H8N2)3]SO4·7.5H2O, is a six-coordinate copper(II) complex with a slightly elongated octa­hedral coordination geometry. The pyridyl rings of the three bipyridyl ligands are not coplanar, making dihedral angels of 9.5 (5), 5.2 (4) and 5.8 (5)°. In the crystal, several O—H⋯O and C—H⋯O hydrogen-bonding inter­actions are observed due to the existance of a large number of water mol­ecules and the sulfate dianions. PMID:21581747

  6. 21 CFR 184.1261 - Copper sulfate.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Copper sulfate. 184.1261 Section 184.1261 Food and... Substances Affirmed as GRAS § 184.1261 Copper sulfate. (a) Copper sulfate (cupric sulfate, CuSO4·5H2O, CAS... the reaction of sulfuric acid with cupric oxide or with copper metal. (b) The ingredient must be of...

  7. 21 CFR 184.1261 - Copper sulfate.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Copper sulfate. 184.1261 Section 184.1261 Food and... Substances Affirmed as GRAS § 184.1261 Copper sulfate. (a) Copper sulfate (cupric sulfate, CuSO4·5H2O, CAS... the reaction of sulfuric acid with cupric oxide or with copper metal. (b) The ingredient must be of...

  8. 21 CFR 184.1261 - Copper sulfate.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Copper sulfate. 184.1261 Section 184.1261 Food and... Substances Affirmed as GRAS § 184.1261 Copper sulfate. (a) Copper sulfate (cupric sulfate, CuSO4·5 H2O, CAS... the reaction of sulfuric acid with cupric oxide or with copper metal. (b) The ingredient must be of...

  9. 21 CFR 184.1261 - Copper sulfate.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Copper sulfate. 184.1261 Section 184.1261 Food and... Substances Affirmed as GRAS § 184.1261 Copper sulfate. (a) Copper sulfate (cupric sulfate, CuSO4·5 H2O, CAS... the reaction of sulfuric acid with cupric oxide or with copper metal. (b) The ingredient must be of...

  10. 21 CFR 184.1261 - Copper sulfate.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Copper sulfate. 184.1261 Section 184.1261 Food and....1261 Copper sulfate. (a) Copper sulfate (cupric sulfate, CuSO4·5 H2O, CAS Reg. No. 7758-99-8) usually... sulfuric acid with cupric oxide or with copper metal. (b) The ingredient must be of a purity suitable...

  11. Status of copper sulfate - 2008

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This is brief overview of the Technical Sections completed and being worked on for the New Animal Drug Application (NADA) for copper sulfate. Initial Label Claim (Ich on catfish): 1) Human Food Safety - Complete for all fin fish – February 2004. This includes human intestinal microflora issues,...

  12. Status of Copper Sulfate - 2010

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This is brief overview of the Technical Sections completed and being worked on for the New Animal Drug Application (NADA) for copper sulfate. Initial Label Claim (Ich on catfish): 1) Human Food Safety - Complete for all fin fish - February 2004. This includes human intestinal microflora issues,...

  13. Catalytic-Oxidative Leaching of Low-Grade Complex Zinc Ore by Cu (II) Ions Produced from Copper Ore in Ammonia-Ammonium Sulfate Solution

    NASA Astrophysics Data System (ADS)

    Liu, Zhi Xiong; Yin, Zhou Lan; Hu, Hui Ping; Chen, Qi Yuan

    2012-10-01

    The catalytic-oxidative leaching of a mixed ore, which consists of low-grade oxide copper ore and oxide zinc ore containing ZnS, was investigated in ammonia-ammonium sulfate solution. The effect of the main parameters, such as mass ratio of copper ore to zinc ore, liquid-to-solid ratio, concentration of lixivant, leaching time, and temperature, was studied. The optimal leaching conditions with a maximum extraction of Cu 92.6 pct and Zn 85.5 pct were determined as follows: the mass ratio of copper ore to zinc ore 4/10 g/g, temperature 323.15 K (50 °C), leaching time 6 hours, stirring speed 500 r/min, liquid-to-solid ratio 3.6/1 cm3/g, concentration of lixivant including ammonia 2.0 mol/dm3, ammonium sulfate 1.0 mol/dm3, and ammonium persulfate 0.3 mol/dm3. It was found that ZnS in the oxide zinc ore could be extracted with Cu(II) ion, which was produced from copper ore and was used as the catalyst in the presence of ammonium persulfate.

  14. A new copper(II) chelate complex with tridentate ligand: Synthesis, crystal and molecular electronic structure of aqua-(diethylenetriamine-N, N‧, N‧‧)-copper(II) sulfate monohydrate and its fire retardant properties

    NASA Astrophysics Data System (ADS)

    Lavrenyuk, H.; Mykhalichko, O.; Zarychta, B.; Olijnyk, V.; Mykhalichko, B.

    2015-09-01

    The crystals of a new aqua-(diethylenetriamine-N, N‧, N‧‧)-copper(II) sulfate monohydrate have been synthesized by direct interaction of solid copper(II) sulfate pentahydrate with diethylenetriamine (deta). The crystal structure of [Cu(deta)H2O]SO4ṡH2O (1) has been determined by X-ray diffraction methods at 100 K and characterized using X-ray powder diffraction pattern: space group P 1 bar, a = 7.2819(4), b = 8.4669(4), c = 8.7020(3) Å, α = 83.590(3), β = 89.620(4), γ = 84.946(4)°, Z = 2. The environment of the Cu(II) atom is a distorted, elongated square pyramid which consists of three nitrogen atoms of the deta molecule and oxygen atom of the water molecule in the basal plane of the square pyramid (the average lengths of the in-plane Cu-N and Cu-O bonds are 2.00 Å). The apical position of the coordination polyhedron is occupied by complementary oxygen atom of the sulfate anion (the length of the axial Cu-O bond is 2.421(1) Å). The crystal packing is governed by strong hydrogen bonds of O-H⋯O and N-H⋯O types. The ab initio quantum-chemical calculations have been performed by the restricted Hartree-Fock method with a basis set 6-31∗G using the structural data of [Cu(deta)H2O]SO4ṡH2O. It has been ascertained that the degenerate d-orbitals of the Cu2+ ion split under the co-action of both the square-pyramidal coordination and the chelation. It is significant that visually observed crystals color (blue-violet) of the [Cu(deta)H2O]SO4ṡH2O complex is in good agreement with the calculated value of wavelength of visible light (λ = 5735 Å) which is closely related to the energy of the absorbed photon (Δ = 2.161 eV). Furthermore, the stereo-chemical aspect of influence of the CuSO4 upon combustibility of modified epoxy-amine polymers has been scrutinized.

  15. Status of copper sulfate research at SNARC

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An overview of the Technical Sections completed and being worked on for the New Animal Drug Application (NADA) for copper sulfate will be given. The change in Sponsorship will also be discussed. The Initial label claim will be “For the treatment of ichthyophthiriasis (Ichthyophthirius multifiliis)...

  16. Minnows get columnaris too; copper sulfate works!

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A study was conducted to compare the therapeutic effects of copper sulfate (CuSO4), when delivered in either a flow-through or static system, on the survival of golden shiner (Notemigonus crysoleucas; Fig. 1A) and fathead minnow (Pimephales promelas; Fig. 1B) infected with Flavobacterium columnare (...

  17. Copper sulfate: Liquid or crystals?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two separate experiments were conducted to evaluate copper toxicity to channel catfish and free-swimming Ichthyophthirius multifiliis or Ich (the stage of Ich that can be treated); the compounds we used were CuSO4 crystals and a non-chelated liquid CuSO4 product. In 96 hr tests conducted in aquaria...

  18. Comparative studies of the effects of copper sulfate and zinc sulfate on serum albumins

    NASA Astrophysics Data System (ADS)

    Plotnikova, O. A.; Melnikov, G. V.; Melnikov, A. G.; Kovalenko, A. V.

    2016-04-01

    The work is devoted to the study of the interaction of heavy metals with bovine serum albumin (BSA) and human serum albumin (HSA), by quenching of the intrinsic fluorescence of proteins and fluorescent probe pyrene by heavy metal ions. Sulfates of copper and zinc (CuSO4, ZnSO4) were taken as the metal salts. The value of the Stern-Volmer constants of quenching of intrinsic fluorescence of proteins and fluorescence probe pyrene reduced from Cu (II) to the Zn (II). It was experimentally found that the copper ions have a greater ability to fluorescence quenching, which is probably associated with the greater availability of protein chromophore groups to copper ions and with adsorbed fluorescent probe pyrene in the protein globule.

  19. Uncovering the Terahertz Spectrum of Copper Sulfate Pentahydrate.

    PubMed

    Ruggiero, Michael T; Korter, Timothy M

    2016-01-21

    Terahertz vibrational spectroscopy has evolved into a powerful tool for the detection and characterization of transition metal sulfate compounds, specifically for its ability to differentiate between various hydrated forms with high specificity. Copper(II) sulfate is one such system where multiple crystalline hydrates have had their terahertz spectra fully assigned, and the unique spectral fingerprints of the forms allows for characterization of multicomponent systems with relative ease. Yet the most commonly occurring form, copper(II) sulfate pentahydrate (CuSO4·5H2O), has proven elusive due to the presence of a broad absorption across much of the terahertz region, making the unambiguous identification of its spectral signature difficult. Here, it is shown that the sub-100 cm(-1) spectrum of CuSO4·5H2O is obscured by absorption from adsorbed water and that controlled drying reveals sharp underlying features. The crystalline composition of the samples was monitored in parallel by X-ray diffraction as a function of drying time, supporting the spectroscopic results. Finally, the terahertz spectrum of CuSO4·5H2O was fully assigned using solid-state density functional theory simulations, helping attribute the additional absorptions that appear after excessive drying to formation of CuSO4·3H2O. PMID:26730508

  20. STATUS OF COPPER SULFATE INITIAL LABEL CLAIM - 2006

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A brief overview of the Technical Sections completed and being worked on for the New Animal Drug Application (NADA) for copper sulfate will be presented. Various aspects of these technical sections will be open for discussion. Copper Sulfate NADA: 1) Human Food Safety - Complete for all fin fis...

  1. The safety of copper sulfate to channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is an economical treatment to control fungus (Saprolegnia spp.) on channel catfish eggs and is widely used by the industry. The purpose of this study was to determine the safety of copper sulfate to channel catfish eggs when treated at the therapeutic rate (10 mg/L), and also...

  2. A Copper-Sulfate-Based Inorganic Chemistry Laboratory for First-Year University Students That Teaches Basic Operations and Concepts.

    ERIC Educational Resources Information Center

    Rodriguez, Emilio; Vicente, Miguel Angel

    2002-01-01

    Presents a 10-hour chemistry experiment using copper sulfate that has three steps: (1) purification of an ore containing copper sulfate and insoluble basic copper sulfates; (2) determination of the number of water molecules in hydrated copper sulfate; and (3) recovery of metallic copper from copper sulfate. (Author/YDS)

  3. Copper Sulfate Foot Baths on Dairies and Crop Toxicities

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A rising concern with the application of dairy wastes to agricultural fields is the accumulation of copper (Cu) in the soil. Copper sulfate (CuSO4) from cattle foot baths are washed out of dairy barns and into wastewater lagoons. The addition of CuSO4 baths has been reported to increase Cu concent...

  4. Changes in copper sulfate crystal habit during cooling crystallization

    NASA Astrophysics Data System (ADS)

    Giulietti, M.; Seckler, M. M.; Derenzo, S.; Valarelli, J. V.

    1996-09-01

    The morphology of technical grade copper(II) sulfate pentahydrate crystals produced from batch cooling experiments in the temperature range of 70 to 30°C is described and correlated with the process conditions. A slow linear cooling rate (batch time of 90 min) predominantly caused the appearance of well-formed crystals. Exponential cooling (120 min) resulted in the additional formation of agglomerates and twins. The presence of seeds for both cooling modes led to round crystals, agglomerates and twins. Fast linear cooling (15 min) gave rise to a mixture of the former types. Broken crystals and adhering fragments were often found. Growth zoning was pronounced in seeded and linear cooling experiments. Fluid inclusions were always found and were more pronounced for larger particles. The occurrence of twinning, zoning and fluid inclusions was qualitatively explained in terms of fundamental principles.

  5. Analysis of Saprolegnia parasitica Transcriptome following Treatment with Copper Sulfate

    PubMed Central

    Ye, Xin; Sun, Qi; Yuan, Hai-Lan; Liang, Nan; Fang, Wen-Hong; Li, Hao-Ran; Yang, Xian-Le

    2016-01-01

    Background Massive infection caused by oomycete fungus Saprolegnia parasitica is detrimental to freshwater fish. Recently, we showed that copper sulfate demonstrated good efficacy for controlling S. parasitica infection in grass carp. In this study, we investigated the mechanism of inhibition of S. parasitica growth by copper sulfate by analyzing the transcriptome of copper sulfate—treated S. parasitica. To examine the mechanism of copper sulfate inhibiting S. parasitica, we utilized RNA-seq technology to compare differential gene expression in S. parasitica treated with or without copper sulfate. Results The total mapped rates of the reads with the reference genome were 90.50% in the control group and 73.50% in the experimental group. In the control group, annotated splice junctions, partial novel splice junctions and complete novel splice junctions were about 83%, 3% and 14%, respectively. In the treatment group, the corresponding values were about 75%, 6% and 19%. Following copper sulfate treatment, a total 310 genes were markedly upregulated and 556 genes were markedly downregulated in S. parasitica. Material metabolism related GO terms including cofactor binding (33 genes), 1,3-beta-D-glucan synthase complex (4 genes), carboxylic acid metabolic process (40 genes) were the most significantly enriched. KEGG pathway analysis also determined that the metabolism-related biological pathways were significantly enriched, including the metabolic pathways (98 genes), biosynthesis of secondary metabolites pathways (42 genes), fatty acid metabolism (13 genes), phenylalanine metabolism (7 genes), starch and sucrose metabolism pathway (12 genes). The qRT-PCR results were largely consistent with the RNA-Seq results. Conclusion Our results indicate that copper sulfate inhibits S. parasitica growth by affecting multiple biological functions, including protein synthesis, energy biogenesis, and metabolism. PMID:26895329

  6. Synthesis and characterization of bis-(2-cyano-1-methyl-3-{2- {{(5-methylimidazol-4-yl)methyl}thio}ethyl)guanidine copper(II) sulfate tetrahydrate

    NASA Astrophysics Data System (ADS)

    Rahardjo, Sentot B.; Endah Saraswati, Teguh; Pramono, Edy; Fitriana, Nur

    2016-02-01

    Complex of copper(II) with 2-cyano-1-methyl-3-{2-{{(5-methylimidazol-4- yl)methyl}thio}ethyl)guanidin(xepamet) had been synthesized in 1 : 4 mole ratio of metal to the ligand in methanol. The complex was characterized by metal analysis, thermal gravimetry/differential thermal analyzer (TG/DTA), molar conductivity meter, (Fourier transform infrared spectroscopy) FT-IR, UV-Vis spectroscopy, and magnetic susceptibility balance. The molar conductivity measurement shows that the complex was 2: 1 for electrolyte and SO42- which was acting as a counter ion. The thermal analysis by Thermogravimetric (TG) indicates that the complex contained four molecules of H2O. The Infrared spectral data indicates that functional groups of (C=N) imidazole and (C-S) are coordinated to the center ion Cu2+. Magnetic moment measurement shows that the complex is paramagnetic with peff = 1.78 ± 0.01 BM. Electronic spectra of the complex show a broad band at 608 nm (16447.23 cm-1) are due to Eg→T2g transition. Based on those of characteristics, The complex formula was estimated as [Cu(xepamet)2]SO4.4H2O. The structure of [Cu(xepamet)2]SO4.4H2O complex is probably square planar.

  7. Safety of copper sulfate to channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is commonly used in the catfish industry to control saprolegniasis (caused by watermolds) on eggs. This study was designed to establish the safety of CuSO4 when applied to hatching troughs containing channel catfish eggs in 26 degrees C flow-through well water at 10, 30, and ...

  8. Copper Sulfate Research at Stuttgart National Aquaculture Research Center

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This is brief overview of the Technical Sections completed and being worked on for the New Animal Drug Application (NADA) for copper sulfate. The Initial label claim will be 'For the treatment of ichthyophthiriasis (Ichthyophthirius multifiliis) on Ictalurid catfish cultured in earthen ponds.' The...

  9. Using copper sulfate to control fungus on fish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is widely used by the catfish industry as an economical treatment to control fungus (Saprolegnia spp.) on catfish eggs. This is an overview of our effectiveness and safety studies. Channel catfish spawns were 24 - 48 hrs old. Comparable portions of a single spawn were place...

  10. Practical use of copper sulfate in catfish production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate has been used in aquaculture for many years to control weeds, algae, snails (which carry catfish trematode), and ecto-parasitic organisms in catfish production. Our research has also shown it to be safe and effective to treat fungus on various fish eggs (catfish, hybrid striped bass,...

  11. Dietary copper sulfate for control of gastrointestinal nematodes in goats

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Prevalence of anthelmintic resistance in goats has necessitated studies for alternative means of gastrointestinal nematode (GIN) control. The objective was to determine the effectiveness of dietary copper sulfate for control of GIN in meat goats. Naturally infected buck kids received 0 (LC), 78 (M...

  12. Environmental assessment of copper sulfate use in channel catfish ponds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is used worldwide as a waterborne disease therapeutant in commercial and ornamental fish culture. However, it is not approved by the U.S. Food and Drug Administration (FDA) for therapeutic use in aquaculture; regulatory action on the use of CuSO4 has been deferred pending the...

  13. Control of Saprolegniasis on sunshine bass eggs with copper sulfate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major obstacle to successful hatchery production is water-mould (Saprolegniasis) growth on eggs. Copper sulfate (CuSO4) is commonly used to control Saprolegnia species in channel catfish hatcheries that use troughs, but the effectiveness of it on fish eggs hatched using different systems was not ...

  14. Pretreating channel catfish with copper sulfate affects susceptibility to columnaris

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Columnaris disease is a costly bacterial disease of commercially grown channel catfish. Three experiments were conducted to evaluate the effects of pre-exposing channel catfish fingerlings to 0, 1/2, 1, or 2 times the recommended copper sulfate rate for 24 hours on their susceptibility to columnaris...

  15. Copper sulfate is an effective, economical, multi-use compound for fish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate has a long and storied history of use in aquaculture. Copper sulfate also has a wide range of uses including aquatic weed control, snail control, and treating fish diseases. A number of studies have been conducted to show copper sulfate efficacy against diseases including columnaris d...

  16. 21 CFR 864.9320 - Copper sulfate solution for specific gravity determinations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Copper sulfate solution for specific gravity... Establishments That Manufacture Blood and Blood Products § 864.9320 Copper sulfate solution for specific gravity determinations. (a) Identification. A copper sulfate solution for specific gravity determinations is a...

  17. 21 CFR 864.9320 - Copper sulfate solution for specific gravity determinations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Copper sulfate solution for specific gravity... Establishments That Manufacture Blood and Blood Products § 864.9320 Copper sulfate solution for specific gravity determinations. (a) Identification. A copper sulfate solution for specific gravity determinations is a...

  18. 21 CFR 864.9320 - Copper sulfate solution for specific gravity determinations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Copper sulfate solution for specific gravity... Establishments That Manufacture Blood and Blood Products § 864.9320 Copper sulfate solution for specific gravity determinations. (a) Identification. A copper sulfate solution for specific gravity determinations is a...

  19. 21 CFR 864.9320 - Copper sulfate solution for specific gravity determinations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Copper sulfate solution for specific gravity... Establishments That Manufacture Blood and Blood Products § 864.9320 Copper sulfate solution for specific gravity determinations. (a) Identification. A copper sulfate solution for specific gravity determinations is a...

  20. 21 CFR 864.9320 - Copper sulfate solution for specific gravity determinations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Copper sulfate solution for specific gravity... Establishments That Manufacture Blood and Blood Products § 864.9320 Copper sulfate solution for specific gravity determinations. (a) Identification. A copper sulfate solution for specific gravity determinations is a...

  1. Bone sialoprotein II synthesized by cultured osteoblasts contains tyrosine sulfate

    SciTech Connect

    Ecarot-Charrier, B.; Bouchard, F.; Delloye, C. )

    1989-11-25

    Isolated mouse osteoblasts that retain their osteogenic activity in culture were incubated with (35S) sulfate. Two radiolabeled proteins, in addition to proteoglycans, were extracted from the calcified matrix of osteoblast cultures. All the sulfate label in both proteins was in the form of tyrosine sulfate as assessed by amino acid analysis and thin layer chromatography following alkaline hydrolysis. The elution behavior on DEAE-Sephacel of the major sulfated protein and the apparent Mr on sodium dodecyl sulfate gels were characteristic of bone sialoprotein II extracted from rat. This protein was shown to cross-react with an antiserum raised against bovine bone sialoprotein II, indicating that bone sialoprotein II synthesized by cultured mouse osteoblasts is a tyrosine-sulfated protein. The minor sulfated protein was tentatively identified as bone sialoprotein I or osteopontin based on its elution properties on DEAE-Sephacel and anomalous behavior on sodium dodecyl sulfate gels similar to those reported for rat bone sialoprotein I.

  2. A hybrid water-splitting cycle using copper sulfate and mixed copper oxides

    NASA Technical Reports Server (NTRS)

    Schreiber, J. D.; Remick, R. J.; Foh, S. E.; Mazumder, M. M.

    1980-01-01

    The Institute of Gas Technology has derived and developed a hybrid thermochemical water-splitting cycle based on mixed copper oxides and copper sulfate. Similar to other metal oxide-metal sulfate cycles that use a metal oxide to 'concentrate' electrolytically produced sulfuric acid, this cycle offers the advantage of producing oxygen (to be vented) and sulfur dioxide (to be recycled) in separate steps, thereby eliminating the need of another step to separate these gases. The conceptual process flow-sheet efficiency of the cycle promises to exceed 50%. It has been completely demonstrated in the laboratory with recycled materials. Research in the electrochemical oxidation of sulfur dioxide to produce sulfuric acid and hydrogen performed at IGT indicates that the cell performance goals of 200 mA/sq cm at 0.5 V will be attainable using relatively inexpensive electrode materials.

  3. Susceptibility of channel catfish, Ictalurus punctatus, to Edwardsiella ictaluri challange following copper sulfate exposure

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channel catfish, Ictalurus punctatus (Rafinesque) with or without a preliminary 24h exposure to 2 mg copper sulfate L-1, were challenged with 7.5 X 106 colony forming units L-1 of Edwardsiella ictaluri to determine the effect of copper sulfate on disease resistance. Catfish previously exposed to co...

  4. Are you keeping all your eggs in one basket? Think copper sulfate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    SNARC has been involved with research on copper sulfate for several proposed label claims; this presentation will be about one of the claims, fungus on catfish eggs. A major obstacle in fish hatcheries is the inevitable fungal growth on eggs. Copper sulfate is commonly used for fungus control in c...

  5. Electroforming copper targets for RTNS-II

    SciTech Connect

    Kelley, W.K.; Dini, J.W.; Logan, C.M.

    1981-02-06

    Copper targets used in RTNS II, which is the world's most intense 14-MeV neutron source, contain water cooling channels for temperature control. There are two methods for fabricating these targets: (1) diffusion bonding a copper panel containing photoetched channels to another copper panel, and (2) an electroforming technique which involves filling the photoetched channels with wax, plating thick copper to seal over the channels and then removing the wax. Development of this latter process and results obtained with it are described.

  6. DNA damage in leukocytes of mice treated with copper sulfate.

    PubMed

    Saleha Banu, B; Ishaq, Mohd; Danadevi, K; Padmavathi, P; Ahuja, Y R

    2004-12-01

    Single stranded DNA breaks induced by copper sulfate (CuSO(4)) in mice has been studied in vivo using Alkaline Single Cell Gel Electrophoresis (Comet assay). Mice were administered orally with doses of 0, 1.25, 2.50, 5.00, 7.50, 10.00 and 12.50 mg/kg body weight (b.wt.) of CuSO4 respectively. The samples of whole blood were collected at 24, 48, 72 h, first week and second week post-treatment and the assay was carried out to determine single strand DNA breaks as represented by comet tail-length. In addition, the sample was used to study the repair efficiency by incubating the samples with RPMI medium for 2 h. Results indicated a significant DNA damage at all the doses after treatment with CuSO4 when compared to controls showing a clear dose-dependent response (p < 0.05). A gradual decrease in the tail-lengths from 48 h post-treatment was observed and by second week, the values returned to control levels at all doses. The study on the repair efficiency indicated that mice treated with all the doses of CuSO4 showed decrease in mean comet tail-length indicating repair efficiency capacity but less when compared to those of controls. The study also reveals that comet assay is a sensitive and rapid method for detecting DNA damage caused by trace metals such as copper (Cu). PMID:15500930

  7. Copper(II) binding properties of hepcidin.

    PubMed

    Kulprachakarn, Kanokwan; Chen, Yu-Lin; Kong, Xiaole; Arno, Maria C; Hider, Robert C; Srichairatanakool, Somdet; Bansal, Sukhvinder S

    2016-06-01

    Hepcidin is a peptide hormone that regulates the homeostasis of iron metabolism. The N-terminal domain of hepcidin is conserved amongst a range of species and is capable of binding Cu(II) and Ni(II) through the amino terminal copper-nickel binding motif (ATCUN). It has been suggested that the binding of copper to hepcidin may have biological relevance. In this study we have investigated the binding of Cu(II) with model peptides containing the ATCUN motif, fluorescently labelled hepcidin and hepcidin using MALDI-TOF mass spectrometry. As with albumin, it was found that tetrapeptide models of hepcidin possessed a higher affinity for Cu(II) than that of native hepcidin. The log K 1 value of hepcidin for Cu(II) was determined as 7.7. Cu(II) binds to albumin more tightly than hepcidin (log K 1 = 12) and in view of the serum concentration difference of albumin and hepcidin, the bulk of kinetically labile Cu(II) present in blood will be bound to albumin. It is estimated that the concentration of Cu(II)-hepcidin will be less than one femtomolar in normal serum and thus the binding of copper to hepcidin is unlikely to play a role in iron homeostasis. As with albumin, small tri and tetra peptides are poor models for the metal binding properties of hepcidin. PMID:26883683

  8. Copper sulfate foot baths on dairies and crop toxicities – What are the risks?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A rising concern with the application of dairy wastes to agricultural fields is the accumulation of copper (Cu) in the soil. Copper sulfate (CuSO4) from cattle foot baths are washed out of dairy barns and into wastewater lagoons. The addition of CuSO4 baths has been reported to increase Cu concent...

  9. Copper Sulfate Foot Baths on Dairies and Crop Toxicities – What are the Risks?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A rising concern with the application of dairy wastes to agricultural fields is the accumulation of copper (Cu) in the soil. Copper sulfate (CuSO4) from cattle foot baths are washed out of dairy barns and into wastewater lagoons. The addition of CuSO4 baths has been reported to increase Cu concent...

  10. SPECIES SENSITIVITY TO COPPER SULFATE: CHANNEL CATFISH AND HYBRID-STRIPED BASS.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate is used extensively in aquaculture as an algicide and a therapeutant for protozoan parasites in commercial and recreational fish ponds. The acute toxicity of copper to many species has been studied however there is no data for hybrid striped bass (female white bass Morone chrysops x ...

  11. Induction of phase II enzymes and hsp70 genes by copper sulfate through the electrophile-responsive element (EpRE): insights obtained from a transgenic zebrafish model carrying an orthologous EpRE sequence of mammalian origin.

    PubMed

    Almeida, Daniela Volcan; Nornberg, Bruna Félix da Silva; Geracitano, Laura A; Barros, Daniela Martí; Monserrat, José Maria; Marins, Luis Fernando

    2010-09-01

    We have evaluated the homology of the electrophile-responsive element (EpRE) core sequence, a binding site for the Nrf2 transcription factor, in the proximal promoters of the mouse and zebrafish glutathione-S-transferase (gst), glutamate cysteine ligase catalytic subunit (gclc) and heat shock protein 70 (hsp70) genes. The EpRE sites identified for both species in the three analyzed genes showed a high similarity with the putative EpRE core sequence. We also produced a transgenic zebrafish model carrying a transgene comprised of the luciferase (luc) reporter gene under transcriptional control of a mouse EpRE sequence. This transgenic model was exposed to copper sulfate, and the reporter gene was significantly activated. The endogenous gst, gclc and hsp70 zebrafish genes were analyzed in the EpRE-Luc transgenic zebrafish and showed an expression pattern similar to that of the reporter transgene used. Our results demonstrate that EpRE is conserved between mouse and zebrafish for detoxification-related genes and that the development of genetically modified models using this responsive element to drive the expression of reporter genes can be an important tool in understanding the action mechanism of aquatic pollutants. PMID:19116768

  12. Optimization and validation of a method for the determination of the refractive index of milk serum based on the reaction between milk and copper(II) sulfate to detect milk dilutions.

    PubMed

    Rezende, Patrícia Sueli; do Carmo, Geraldo Paulo; Esteves, Eduardo Gonçalves

    2015-06-01

    We report the use of a method to determine the refractive index of copper(II) serum (RICS) in milk as a tool to detect the fraudulent addition of water. This practice is highly profitable, unlawful, and difficult to deter. The method was optimized and validated and is simple, fast and robust. The optimized method yielded statistically equivalent results compared to the reference method with an accuracy of 0.4% and quadrupled analytical throughput. Trueness, precision (repeatability and intermediate precision) and ruggedness are determined to be satisfactory at a 95.45% confidence level. The expanded uncertainty of the measurement was ±0.38°Zeiss at the 95.45% confidence level (k=3.30), corresponding to 1.03% of the minimum measurement expected in adequate samples (>37.00°Zeiss). PMID:25863391

  13. Effect of copper sulfate upon gill secretory cell nature, mucin production and mucin composition in Mytilus edulis with notes on Modiolus modiolus

    SciTech Connect

    Clements, J.R.

    1983-01-01

    Mussels secrete increased amounts of mucin upon exposure to copper sulfate concentrations equal to or greater than 3 ppm. The threshold level of copper necessary to elicit this response is higher if the animals are exposed to copper sulfate in filtered rather than in unfiltered seawater. At high levels of copper sulfate there is an increase in the relative hydration of the gill tissue.

  14. Toxicity of copper sulfate and rotenone to Chinese mystery snail (Bellamya chinensis)

    USGS Publications Warehouse

    Haak, Danielle M.; Stephen, Bruce J.; Kill, Robert A.; Smeenk, Nicholas A.; Allen, Craig R.; Pope, Kevin L.

    2014-01-01

    The Chinese mystery snail (Bellamya chinensis) is a freshwater snail native to Southeast Asia, Japan, and Russia and is currently classified as an invasive species in at least 27 states in the USA. The species tolerates a wide range of environmental conditions, making management of established populations difficult. We tested the efficacy of two traditional chemical treatments, rotenone and copper sulfate, on the elimination of adult Chinese mystery snails in laboratory experiments. All snails (N=50) survived 72-hour exposure to rotenone-treated lake water, and 96% (N=25) survived 72-hour exposure to pre-determined rotenone concentrations of 0.25, 2.5, and 25.0 mg/L. All snails (N=10) survived exposure to 1.25 mg/L copper sulfate solution, 90% (N=10) survived exposure to 2.50 mg/L copper sulfate solution, and 80% (N=5) survived exposure to 5.0 mg/L copper sulfate solution. Neither rotenone nor copper sulfate effectively killed adult Chinese mystery snails in laboratory experiments, most likely due to their relatively large size, thick shell, and operculum. Therefore, it appears that populations will be very difficult to control once established, and management should focus on preventing additional spread or introductions of this species.

  15. Anodonta imbecillis copper sulfate reference toxicant test, Clinch River - Environmental Restoration Program (CR-ERP)

    SciTech Connect

    Simbeck, D.J.

    1997-06-01

    Reference toxicant testing using juvenile freshwater mussels was conducted as part of the CR-ERP biomonitoring study of Clinch River sediments to assess the sensitivity of test organisms and the overall performance of the test. Tests were conducted using moderately hard synthetic water spiked with known concentrations of copper as copper sulfate. Toxicity testing of copper sulfate reference toxicant was conducted from May 12-21, 1993. The organisms used for testing were juvenile fresh-water mussels (Anodonta imbecillis). Results from this test showed an LC{sub 50} value of 1.12 mg Cu/L which is lower than the value of 2.02 mg Cu/L obtained in a previous test. Too few tests have been conducted with copper as the toxicant to determine a normal range of values.

  16. Nickel, manganese and copper removal by a mixed consortium of sulfate reducing bacteria at a high COD/sulfate ratio.

    PubMed

    Barbosa, L P; Costa, P F; Bertolino, S M; Silva, J C C; Guerra-Sá, R; Leão, V A; Teixeira, M C

    2014-08-01

    The use of sulfate-reducing bacteria (SRB) in passive treatments of acidic effluents containing heavy metals has become an attractive alternative biotechnology. Treatment efficiency may be linked with the effluent conditions (pH and metal concentration) and also to the amount and nature of the organic substrate. Variations on organic substrate and sulfate ratios clearly interfere with the biological removal of this ion by mixed cultures of SRB. This study aimed to cultivate a mixed culture of SRB using different lactate concentrations at pH 7.0 in the presence of Ni, Mn and Cu. The highest sulfate removal efficiency obtained was 98 %, at a COD/sulfate ratio of 2.0. The organic acid analyses indicated an acetate accumulation as a consequence of lactate degradation. Different concentrations of metals were added to the system at neutral pH conditions. Cell proliferation and sulfate consumption in the presence of nickel (4, 20 and 50 mg l(-1)), manganese (1.5, 10 and 25 mg l(-1)) and copper (1.5, 10 and 25 mg l(-1)) were measured. The presence of metals interfered in the sulfate biological removal however the concentration of sulfide produced was high enough to remove over 90 % of the metals in the environment. The molecular characterization of the bacterial consortium based on dsrB gene sequencing indicated the presence of Desulfovibrio desulfuricans, Desulfomonas pigra and Desulfobulbus sp. The results here presented indicate that this SRB culture may be employed for mine effluent bioremediation due to its potential for removing sulfate and metals, simultaneously. PMID:24710619

  17. Effects of copper sulfate supplement on growth, tissue concentration, and ruminal solubilities of molybdenum and copper in sheep fed low and high molybdenum diets

    SciTech Connect

    Ivan, M.; Veira, D.M.

    1985-01-01

    Each of four groups of six wethers were fed one of a low molybdenum, high molybdenum, high molybdenum plus copper sulfate, or high molybdenum plus copper sulfate corn silage-based diet for ad libitum intake for 221 days. Average daily gains and ratios of feed/gain were depressed for the high molybdenum diet as compared with the low molybdenum diet suggesting molybdenum toxicity in sheep fed the high molybdenum diet. This was alleviated partly by the copper sulfate supplement. The supplement also decreased solubility of both copper and molybdenum in the rumen but had no effect on copper concentration in blood plasma. Concentration of molybdenum was higher in both liver and kidney in sheep fed high-molybdenum diets as compared with low-molybdenum diets. Copper concentration was higher in kidneys of sheep fed high-molybdenum diets, but no difference was significant in liver copper between sheep fed diets high or low in molybdenum.

  18. The effect of copper sulfate, potassium permanganate, and peracetic acid on Ichthyobodo necator in channel catfish

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ichthyobodo necator is a single celled biflagellate that can cause significant mortalities in fish, particularly young, tank-reared fish. Copper sulfate (CuSO4), potassium permanganate (KMnO4) and peracetic acid (PAA) were evaluated for effectiveness against Ichthybodosis in juvenile channel catfis...

  19. Effectiveness of copper sulfate and potassium permanganate on channel catfish infected with Flavobacterium columnare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were evaluated for their effectiveness to curtail mortality and decrease bacterial load in fish tissues and water in channel catfish Ictalurus punctatus naturally infected with Flavobacterium columnare, the causative agent of columnaris. Fis...

  20. Copper sulfate toxicity to two isolates of Ichthyophthirius multifiliis relative to alkalinity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Theronts from two different strains of Ichthyophthirius multifiliis (AR1 and G5) were exposed to copper sulfate in waters of different total alkalinities and observed for 4 h to determine relative toxicity and kinetics of parasite mortality. Consistent with the known solubility properties of the me...

  1. Comparing copper sulfate, diquat bromide, formalin, and hydrogen peroxide treatments on channel catfish eggs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Reduced fish egg survival is often associated with fungal infestation (Saprolegnia spp.) of the eggs. Chemical treatments have been used to limit these infestations on fish eggs and increase survival. The effect of copper sulfate (10 mg/L), diquat bromide (25 mg/L diquat cation), formalin (433 mg/...

  2. Treating a natural outbreak of columnaris in channel catfish with copper sulfate and potassium permanganate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An F. Columnare-exclusive epizootic occurred in fingerling channel catfish (Ictalurus punctatus) during normal tank culture practices at SNARC. Fish were transferred to the ultra low-flow system and 2.1 mg/L copper sulfate or 3 mg/L potassium permanganate was administered; an untreated control was ...

  3. The use of copper sulfate in catfish hatcheries: safety and effectiveness

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is cheap, safe to the applicator and effective for controlling fungus on catfish eggs. Several studies were designed to determine the effectiveness and safety of CuSO4 to channel catfish eggs in pursuit of an FDA approval. In an effectiveness study for the FDA, we found that ...

  4. The use of copper sulfate in channel catfish hatcheries: Safety and effectiveness

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is an economical treatment to control fungus (Saprolegnia spp.) on channel catfish eggs and is widely used by the industry. Several studies were designed to determine the effectiveness and safety of CuSO4 to channel catfish eggs in pursuit of an FDA-approval. Effectiveness:...

  5. Using a computer model to calculate copper sulfate treatments for Ich

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is often used to control infestations of Ichthyophthirius multifiliis (Ich) in pond aquaculture in the United States. In this study, we determined the acute toxicity of CuSO4 to the free-swimming theronts of Ich in reconstituted waters. Water chemistry characteristics, inclu...

  6. Egg saprolegniasis in a commercial sunshine bass hatchery: Control regime developed using copper sulfate

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An obstacle to sunshine bass (female white bass Morone chrysops × male striped bass M. saxatilis) larval production is fungal growth on eggs caused by the water-mold Saprolegnia spp. Copper sulfate (CuSO4) is commonly used for fungus control in troughs of catfish hatcheries, but the effectiveness o...

  7. Using copper sulfate to control egg fungus at Keo Fish Farm

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Keo Fish Farm is the biggest producer of hybrid striped bass fry in the world. The hatchery manager asked about treatments to control fungus on eggs which occurred fairly often. Our lab has been working on gaining FDA-approval to use copper sulfate to control fungus on catfish eggs, so we were con...

  8. Using copper sulfate to control fungus on eggs in catfish hatcheries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Copper sulfate (CuSO4) is used by the catfish industry for controlling parasites and as an economical treatment to control fungus (Saprolegnia spp.) on channel catfish eggs. Several studies were designed at SNARC to evaluate the effectiveness and safety of CuSO4 to channel catfish eggs in pursuit o...

  9. DEVELOPMENTAL TOXICITY OF COPPER SULFATE AND METHYLENE CHLORIDE TO SHRIMP EMBRYOS

    EPA Science Inventory

    The embryos of the grass shrimp (Palaemonetes pugio) have shown sensitivity to the water-soluble fraction of Number 2 fuel oil which indicates they may be a useful test species in estuarine developmental toxicity tests. Detailed concentration-response curves for copper sulfate an...

  10. Treating sunshine bass eggs with copper sulfate controls fungus and increases survival

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major obstacle to sunshine bass production is fungal growth on eggs. Copper sulfate (CuSO4) is commonly used for fungus control in channel catfish hatcheries that use troughs, but the effectiveness of it on fish eggs hatched using different systems was not known. Female white bass Morone chrysop...

  11. Using copper sulfate on hybrid striped bass eggs to control fungus and increase survival

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major obstacle in fish hatcheries is the inevitable fungal growth on eggs. Copper sulfate (CuSO4) is commonly used for fungus control in channel catfish hatcheries that use troughs, but effectiveness on fish eggs hatched using different systems has only recently been investigated. Fish were spawn...

  12. Copper sulfate controls fungus on sunshine bass eggs and increases survival

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major obstacle to sunshine bass production is fungal growth on eggs. Copper sulfate (CuSO4) is commonly used for fungus control in channel catfish hatcheries that use troughs, but the effectiveness of it on fish eggs hatched using different systems was not known. Female white bass Morone chrysop...

  13. Use of copper sulfate to control Saprolegniasis at a commercial sunshine bass hatchery

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An obstacle to sunshine bass (female white bass Morone chrysops × male striped bass M. saxatilis) larval production is fungal growth on eggs caused by the water-mold Saprolegnia spp. Copper sulfate (CuSO4) is commonly used for fungus control in troughs of catfish hatcheries, but the effectiveness o...

  14. Using copper sulfate on hybrid striped bass eggs to control fungus and increase survival

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A major obstacle in fish hatcheries is reduced hatch rates due to fungal growth on eggs. Copper sulfate (CuSO4) is commonly used for fungus control in catfish hatcheries that use troughs, but effectiveness on other species of fish eggs in different hatching systems has only recently been investigat...

  15. [Molluscacide activity of a mixture of 6-n-alkyl salicylic acids (anacardic acid) and 2 of its complexes with copper (II) and lead (II)].

    PubMed

    Mendes, N M; de Oliveira, A B; Guimarães, J E; Pereira, J P; Katz, N

    1990-01-01

    The molluscicide activity of hexanic extract from Anacardium occidentale L. (cashew) nut shell, of copper (II) complex, of lead (II) complex and anacardic acid has been compared in the laboratory in an attempt to obtain better stability than anacardic acid. This was obtained from the hexanic extract of the cashew nut shell by precipitation with lead (II) hydroxide or cupric sulfate plus sodium hydroxide or (II) cupric hydroxide followed by treatment of lead (II) complex with a diluted solution of sulfuric acid. Ten products of the mixture obtained were tested on adults snails of Biomphalaria glabrata at 1 to 10 ppm. The most active products were copper (II) complex, obtained by cupric sulfate plus sodium hydroxide, and anacardic acid (sodium hydroxide) which presented activity at 4 ppm. The anacardic acid's lead content was above the limits accepted by the United States standards. PMID:2133588

  16. Novel copper (II) alginate hydrogels and their potential for use as anti-bacterial wound dressings.

    PubMed

    Klinkajon, Wimonwan; Supaphol, Pitt

    2014-08-01

    The incorporation of a metal ion, with antimicrobial activity, into an alginate dressing is an attractive approach to minimize infection in a wound. In this work, copper (II) cross-linked alginate hydrogels were successfully prepared using a two-step cross-linking procedure. In the first step, solid alginate films were prepared using a solvent-casting method from soft gels of alginate solutions that had been lightly cross-linked using a copper (II) (Cu(2+)) sulfate solution. In the second step, the films were further cross-linked in a corresponding Cu(2+) sulfate solution using a dipping method to further improve their dimensional stability. Alginate solution (at 2%w/v) and Cu(2+) sulfate solution (at 2%w/v) in acetate buffer at a low pH provided soft films with excellent swelling behavior. An increase in either Cu(2+) ion concentration or cross-linking time led to hydrogels with more densely-cross-linked networks that limited water absorption. The hydrogels clearly showed antibacterial activity against Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis and Streptococcus pyogenes, which was proportional to the Cu(2+) ion concentration. Blood coagulation studies showed that the tested copper (II) cross-linked alginate hydrogels had a tendency to coagulate fibrin, and possibly had an effect on pro-thrombotic coagulation and platelet activation. Conclusively, the prepared films are likely candidates as antibacterial wound dressings. PMID:25029588

  17. Comparison of the toxicity of two chelated copper algaecides and copper sulfate to non-target fish.

    PubMed

    Closson, K R; Paul, E A

    2014-12-01

    New pesticide products are reviewed by the United States Environmental Protection Agency for possible effects to non-target aquatic organisms. The required toxicity data are for the active ingredient only, and fail to include toxicity of the mixture of other ingredients found in these pesticides. These ingredients may increase the toxicity of the active ingredient to non-target organisms. Our study compares the toxicity of two formulations of chelated copper algaecides with each other, and to a copper sulfate algaecide. We were particularly interested in the effects of a surfactant that is present in one of the formulations. We found that copper becomes less toxic to fish (e.g. fathead minnow 48-h LC50 = 0.90 mg/L) when it is chelated, providing an additional margin of safety to non-target fish compared to copper sulfate. However, inclusion of a surfactant to the formulation resulted in increased toxicity (e.g. fathead minnow 48-h LC50 = 0.30 mg/L). PMID:25283368

  18. Effects of copper sulfate on seedlings of Prosopis pubescens (screwbean mesquite).

    PubMed

    Zappala, Marian N; Ellzey, Joanne T; Bader, Julia; Peralta-Videa, Jose R; Gardea-Torresdey, Jorge

    2014-01-01

    Phytoextraction is an established method of removal of heavy metals from contaminated soils worldwide. Phytoextraction is most efficient if local plants are used in the contaminated site. We propose that Prosopis pubescens (Screw bean mesquite) would be a successful phytoextractor of copper in our local soils. In order to determine the feasibility of using Screw bean mesquite, we utilized inductively-coupled plasma-optical emission spectroscopy (ICP-OES) and elemental analysis to observe the uptake of copper and the effects on macro and micro nutrients within laboratory-grown seedlings. We have previously shown that P. pubescens is a hyperaccumulator of copper in soil-grown seedlings. Light and transmission electron microscopy demonstrated death of root cells and ultrastructural changes due to the presence of copper from 50 mg/L - 600 mg/L. Ultrastructural changes included plasmolysis, starch accumulation, increased vacuolation and swollen chloroplasts with disarranged thylakoid membranes in cotyledons. Inductively coupled plasma-optical emission spectroscopy analyses of macro- and micro-nutrients revealed that the presence of copper sulfate in the growth medium of Petri-dish grown Prosopis pubescens seedlings resulted in dramatic decreases of magnesium, potassium and phosphorus. At 500-600 mg/L of copper sulfate, a substantial increase of sulfur was present in roots. PMID:24933900

  19. Copper-Sulfate Pentahydrate as a Product of the Waste Sulfuric Acid Solution Treatment

    NASA Astrophysics Data System (ADS)

    Marković, Radmila; Stevanović, Jasmina; Avramović, Ljiljana; Nedeljković, Dragutin; Jugović, Branimir; Stajić-Trošić, Jasna; Gvozdenović, Milica

    2012-12-01

    The aim of this study is synthesis of copper-sulfate pentahydrate from the waste sulfuric acid solution-mother liquor generated during the regeneration process of copper bleed solution. Copper is removed from the mother liquor solution in the process of the electrolytic treatment using the insoluble lead anodes alloyed with 6 mass pct of antimony on the industrial-scale equipment. As the result of the decopperization process, copper is removed in the form of the cathode sludge and is precipitated at the bottom of the electrolytic cell. By this procedure, the content of copper could be reduced to the 20 mass pct of the initial value. Chemical characterization of the sludge has shown that it contains about 90 mass pct of copper. During the decopperization process, the very strong poison, arsine, can be formed, and the process is in that case terminated. The copper leaching degree of 82 mass pct is obtained using H2SO4 aqueous solution with the oxygen addition during the cathode sludge chemical treatment at 80 °C ± 5 °C. Obtained copper salt satisfies the requirements of the Serbian Standard for Pesticide, SRPS H.P1. 058. Therefore, the treatment of waste sulfuric acid solutions is of great economic and environmental interest.

  20. Relative bioavailability of copper in tribasic copper chloride to copper in copper sulfate for laying hens based on egg yolk and feather copper concentrations.

    PubMed

    Kim, J W; Kim, J H; Shin, J E; Kil, D Y

    2016-07-01

    This experiment was conducted to determine the relative bioavailability (RBV) of Cu in tribasic copper chloride (TBCC) to Cu in copper sulfate (monohydrate form; CuSO4·H2O) for layer diets based on egg yolk and feather Cu concentrations. A total of 252, 72-wk-old Hy-Line Brown laying hens were allotted to 1 of 7 treatments with 6 replicates consisting of 6 hens per replicate in a completely randomized design. Hens were fed corn-soybean meal-based basal diets supplemented with 0 (basal), 100, 200, or 300 mg/kg Cu from CuSO4 or TBCC for 4 wk. Results indicated that egg production, egg weight, and egg mass were not affected by dietary treatments. However, increasing inclusion levels of Cu in diets from CuSO4 decreased (P < 0.05) feed conversion ratio (FCR), whereas increasing inclusion levels of Cu in diets from TBCC did not affect FCR, indicating significant interaction (P < 0.05). Increasing inclusion levels of Cu from TBCC or CuSO4 increased (P < 0.05) Cu concentrations of egg yolk and feathers. Feather Cu concentrations were greater (P < 0.01) for hens fed diets containing CuSO4 than for hens fed diets containing TBCC. The values for the RBV of Cu in TBCC to Cu in CuSO4 based on log10 transformed egg yolk and feather Cu concentrations were 107.4% and 69.5%, respectively. These values for the RBV of Cu in TBCC did not differ from Cu in CuSO4 (100%). The RBV measured in egg yolk did not differ from the RBV measured in feather. In conclusion, the RBV of Cu in TBCC to Cu in CuSO4 can be determined using Cu concentrations of egg yolk and feathers although the values depend largely on target tissues of laying hens. For a practical application, however, the RBV value of Cu in TBCC to Cu in CuSO4 could be 88.5% when the RBV values determined using egg yolk and feather Cu concentrations were averaged. PMID:26944968

  1. Pharmacokinetic study of copper (II) acetylsalicylate.

    PubMed

    Iqbal, Mohammad S; Sher, Muhammad; Pervez, Humayun; Saeed, Maryiam

    2008-09-01

    This study was aimed at determination of pharmacokinetic parameters of copper (II) acetylsalicylate (CAS). Ten volunteers received a 60-mg dose of CAS. Blood samples were collected just before and after 0.25, 0.5, 0.75, 1.0, 1.5, 2.5, 3.0, 3.5, 4.0, 4.5, 5.5, 7.0, 10, and 12.0 h of administration of the drug. The plasma samples were analyzed for CAS and its metabolites by a validated high-performance liquid chromatography method having a suitable lower limit of quantification. The dose of 60 mg was well tolerated without any adverse effect. The maximum plasma concentration of CAS was found to be 0.38 mg L(-1) with t (max) of 0.72 h. The plasma half-life, clearance, and volume of distribution of CAS were 8.67 h, 66.30 L h(-1) and 829 L kg(-1), respectively. The elimination of CAS, acetylsalicylic acid, copper salicylate, and salicylic acid follows the first order kinetics with r (2) 0.979, 0.880, 0.991, and 0.998, respectively. The study provided for the first time the pharmacokinetic data for CAS after oral administration of CAS. The data were found to be useful in understanding the claimed enhanced anti-inflammatory activity of the drug as compared with that of acetylsalicylic acid. PMID:18478192

  2. Resistance of pulmonate snail populations to repeated treatments of copper sulfate

    NASA Astrophysics Data System (ADS)

    Blankespoor, Harvey D.; Cameron, Stephen C.; Cairns, John

    1985-09-01

    Two species of pulmonate snails, Lymnaea cat-ascopium and Physa integra, were collected from Douglas and Houghton Lakes. Snail populations from the former lake (pristine) had never been exposed to copper sulfate, whereas those from the latter one (treated) had been subjected to the molluscicide for more than 40 years. Molluscs from the treated lake were more resistant to the copper at concentrations of 0.5 and 1.0 ppm than those from the pristine lake. Furthermore, larger lymnaeids had a higher survival rate than smaller ones.

  3. Crystal structures of copper(II) nitrate, copper(II) chloride, and copper(II) perchlorate complexes with 2-formylpyridine semicarbazone

    SciTech Connect

    Chumakov, Yu. M.; Tsapkov, V. I.; Antosyak, B. Ya.; Bairac, N. N.; Simonov, Yu. A.; Bocelli, G.; Pahontu, E.; Gulea, A. P.

    2009-05-15

    Compounds dinitrato(2-formylpyridinesemicarbazone)copper (I), dichloro(2-formylpyridinesemicarbazone) copper hemihydrate (II), and bis(2-formylpyridinesemicarbazone)copper(2+) perchlorate hydrate (III) are synthesized and their crystal structures are determined. In compounds I-III, the neutral 2-formylpyridine semicarbazone molecule (L) is tridentately attached to the copper atom via the N,N,O set of donor atoms. In compounds I and II, the Cu: L ratio is equal to 1: 1, whereas, in III, it is 1: 2. In complex I, the coordination sphere of the copper atom includes two nitrate ions with different structural functions in addition to the L ligand. The structure is built as a one-dimensional polymer in which the NO{sub 3} bidentate group fulfills a bridging function. The coordination polyhedron of the copper(2+) atom can be considered a distorted tetragonal bipyramid (4 + 1 + 1). Compound II has an ionic structure in which the main element is the [CuLCl{sub 2} . Cu(H{sub 2}O)LCl]{sup +} dimer. In the dimer, the copper atoms are linked via one of the {mu}{sub 2}-bridging chlorine atoms. The coordination polyhedra of the central atoms of the Cu(H{sub 2})LCl and CuLCl{sub 2} complex fragments are tetragonal bipyramid and tetragonal pyramid, respectively. In compound III, the copper atom is octahedrally surrounded by two L ligands in the mer configuration.

  4. Stimulation of sulfated glycosaminoglycan synthesis by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+.

    PubMed

    Wegrowski, Y; Maquart, F X; Borel, J P

    1992-01-01

    Glycyl-L-histidyl-L-lysine-copper (II) complex (GHK-Cu) is a naturally occurring tripeptide with potential healing properties. We studied the effect of GHK-Cu on the synthesis of glycosaminoglycans (GAGs) by normal human fibroblasts in culture. Cells were incubated with 3H glucosamine and 35S sulfate and the radioactivity of isolated GAGs was determined. GHK-Cu induced a dose-dependent increase of the synthesis of total GAGs secreted into the culture medium and those associated with the cell layer. The effect of GHK-Cu was biphasic with a maximal stimulation at 10(-9) to 10(-8) M. At higher concentrations, the rate of synthesis returned progressively to that of control cultures. Electrophoretic analysis of the different GAG populations showed that GHK-Cu preferentially stimulated the synthesis of extracellular dermatan sulfate and cell layer associated heparan sulfate. No influence of GHK-Cu on the synthesis of hyaluronic acid was observed. GHK-Cu stimulation of GAG synthesis may be one of the phenomenons implicated in the wound healing properties of the peptide. PMID:1522753

  5. Removal of copper in an integrated sulfate reducing bioreactor-crystallization reactor system.

    PubMed

    Sierra-Alvarez, Reyes; Hollingsworth, Jeremy; Zhou, Michael S

    2007-02-15

    Removal of copper was investigated using an innovative water treatment system integrating a sulfidogenic bioreactor with a fluidized-bed crystallization reactor containing fine sand to facilitate the recovery of copper as a purified copper-sulfide mineral. The performance of the system was tested using a simulated semiconductor manufacturing wastewater containing high levels of Cu2+ (4-66 mg/L), sulfate, and a mixture of citrate, isopropanol, and polyethylene glycol (Mn 300). Soluble copper removal efficiencies exceeding 99% and effluent copper concentrations averaging 89 micog/L were demonstrated in the two-stage system, with near complete metal removal occurring in the crystallizer. Copper crystals deposited on sand grains were identified as covellite (CuS). The removal of organic constituents did not exceed 70% of the initial chemical oxygen demand due to incomplete degradation of isopropanol and its breakdown product (acetone). Taken as a whole, these results indicate the potential of this novel reactor configuration for the simultaneous removal of heavy metals and organic constituents. The ability of this process to recover heavy metals in a purified form makes it particularly attractive for the treatment of contaminated aqueous streams, including industrial wastewaters and acid mine drainage. PMID:17593752

  6. X-ray photoelectron spectroscopy of copper(II), copper(I), and mixed valence systems.

    PubMed

    Rupp, H; Weser, U

    1976-01-01

    X-ray photoelectron spectroscopy using copper(II), copper(I) and the mixed valence Cu(II)/Cu(I) compounds was employed as a means of studying electron transfer reactions in copper proteins. The X-ray photoelectron spectra of copper(II) compounds display characteristic satellites of both variable size and resolution. Some of these satellites could be assigned to specific ligand interactions. Unlike electron paramagnetic resonance spectroscopy, the X-ray photoelectron spectroscopic measurements of copper(I) compounds allowed the unequivocal assignment of this oxidation state. No satellites at all could be detected in the Cu(I) spectra. Furthermore, established mixed valence Cu(II)/Cu(I) complexes including Cu2SO3-CuSO3-2H2O and Cu4Cl5 (ethylenediamine)2 proved essentially a mixture of distinct portions of Cu(I) and Cu(II). This indicates that both oxidation states of copper survive in such complexes. In contrast, all Cu X-ray photoelectron signals of the more tentatively described mixed valence complexes Na2Cu3S3 and the mineral covellite, CuI4CuII2(S2)2S2, could be attributed exclusively to Cu(I). In view of the known binding of copper with sulfur in many copper proteins, it was of utmost importance to study the copper-sulfur interactions. We have demonstrated the absence of Cu(II) in CuS. This indicates strong metal-induced polarization of sulfur resulting in electron transfer to copper to yield Cu(I). PMID:953045

  7. Investigation of copper(II) tetrafluoroborate catalysed epoxide opening

    PubMed Central

    Capes, Amy S.; Crossman, Arthur T.; Webster, Lauren A.; Ferguson, Michael A.J.; Gilbert, Ian H.

    2011-01-01

    We report the extension of the copper(II) tetrafluoroborate catalysed opening of epoxides with alcohols to include a wider variety of alcohols, a range of solvents and a method to purify the products from the reaction. PMID:22505782

  8. Pyridine-copper(ii) formates for the generation of high conductivity copper films at low temperatures.

    PubMed

    Paquet, C; Lacelle, T; Deore, B; Kell, A J; Liu, X; Korobkov, I; Malenfant, P R L

    2016-02-11

    Pyridine derivatives coordinated to copper(ii) formates are shown to have lower decomposition temperatures than the alkylamine analogues. Using heating profiles compatible with low temperature substrates, deposited inks made from these compounds are transformed into copper traces with a resistivity value of 14 μΩ cm when sintered at 135 °C in <5 minutes. PMID:26750775

  9. Electrochemical treatment of acidic aqueous ferrous sulfate and copper sulfate as models for acid mine drainage.

    PubMed

    Bunce, N J; Chartrand, M; Keech, P

    2001-12-01

    Acid mine drainage (AMD) is a serious environmental problem in the mining industry. The present work describes electrolytic reduction of solutions of synthetic AMD, comprising FeSO4/H2SO4 and CuSO4/H2SO4, in flow-through cells whose anode and cathode compartments were separated using ion exchange membranes. In the case of FeSO4/H2SO4 at constant flow rate, the pH of the effluent from the catholyte increased progressively with current at a variety of cathodes, due to electrolytic reduction of H+ ions to elemental hydrogen. Near-quantitative removal of iron was achieved by sparging air into the catholyte effluent, thereby precipitating iron outside the electrochemical cell, and avoiding fouling of the electrodes. The anode reaction was the oxidation of water to O2, a proton-releasing process. Using cation exchange membranes and sodium sulfate as the supporting electrolyte in the anode compartment, the efficiency of the process was compromised at high currents by transport of H+ competitively with Na+ from the anode to the cathode compartments. Higher efficiencies were obtained when anion exchange membranes were used, and in this case no additional supporting electrolyte other than dilute H2SO4 was needed, the net reaction being the electrochemically driven transfer of the elements of H2SO4 from the cathode to the anode compartments. Current efficiencies approximately 50% were achieved, the loss of efficiency being accounted for by ohmic heating of the solutions. In the case of CuSO4/H2SO4 and anion exchange membranes at high currents, reduction of Cu2+ and H+ ions and transport of SO4(2-) ions out of the catholyte caused unacceptably high potentials to be generated. PMID:11763043

  10. A Hexameric Cationic Copper(II) Metallacrown as a Pertechnetate and Perrhenate Scavenger.

    PubMed

    Rodríguez-Hermida, Sabina; Lago, Ana Belén; Pino-Cuevas, Arantxa; Hagenbach, Adelheid; Cañadillas-Delgado, Laura; Carballo, Rosa; Abram, Ulrich; Vázquez-López, Ezequiel M

    2016-01-26

    Materials based on the cationic copper(II) hexanuclear 18-membered metallacrown [18-MCCuII-N(2ph)-6](6+) (2phH=2-piconyl hydrazide) and tetrafluoroborate, perchlorate, nitrate, sulfate, and perrhenate anions were prepared by an easy method in aqueous medium. Single-crystal X-ray characterization of six members of this new family of complexes showed that the anions are attached to the metallacrown by direct coordination to a copper cation or by hydrogen-bonding interaction with the center of the hexamer. The stable cationic nature of the complexes and their ability to bind different anions allows them to adsorb and immobilize environmentally relevant anions such as MO4(-) (M=Tc, Re). The MO4(-) trapping capacities suggest that these materials would be useful in the treatment of oxoanionic contaminants in water. PMID:26671639

  11. Water soluble sodium sulfate nanorods as a versatile template for the designing of copper sulfide nanotubes.

    PubMed

    Das, Gautam; Kakati, Nitul; Lee, Seok Hee; Karak, Niranjan; Yoon, Young Soo

    2014-06-01

    The present study reports the use of water soluble sodium sulfate (Na2SO4) nanorods as a versatile template for generation of tubular copper sulfide (CuS) nanostructures. The Na2SO4 nanorods were synthesized from ammonium sulfate (NH4)2SO4 and sodium hydroxide (NaOH), under refluxing condition. The shape and morphology control of the Na2SO4 nanorods were studied with respect to nature of surfactant used and reactant mole ratio. While, PVP mole ratio was important to obtain homogeneous nanorods. Uniform and stable nanotubes of CuS were than obtained by the dissolution of the nanorods in water. The use of simple chemicals for synthesis of such nanotube templates opens the prospect for wide scale downstream applications. PMID:24738412

  12. Effect of dietary copper sulfate, Aureo SP250, or clinoptilolite on ureolytic bacteria found in the pig large intestine.

    PubMed Central

    Varel, V H; Robinson, I M; Pond, W G

    1987-01-01

    The predominant ureolytic bacteria in the pig large intestine were determined while growing pigs were fed a basal diet or basal diet plus copper sulfate, Aureo SP250, or clinoptilolite. Fecal samples were collected from four pigs fed each diet at 3, 9, and 14 weeks and analyzed for total colony counts and percent ureolytic bacteria. Fecal urease activity, ammonia nitrogen, and identity of the ureolytic bacteria were determined at 14 weeks. Copper sulfate and Aureo SP250 reduced the number of ureolytic organisms, with a marked decrease occurring in the Streptococcus spp., which made up 74% of the ureolytic isolates from the pigs on the basal diet. Other ureolytic species detected at lower concentrations were Staphylococcus spp., Selenomonas ruminantium, Bacteroides multiacidus, and Eubacterium limosum. Copper sulfate also reduced fecal urease activity (P less than 0.10). Fecal ammonia concentrations were not different between pigs fed the various diets. These data suggest that the streptococci are the most numerous ureolytic species in the pig intestinal tract and are significantly reduced by copper sulfate and Aureo SP250; however, only copper sulfate reduced intestinal urease activity. PMID:2823707

  13. Effect of dietary copper sulfate, Aureo SP250, or clinoptilolite on ureolytic bacteria found in the pig large intestine.

    PubMed

    Varel, V H; Robinson, I M; Pond, W G

    1987-09-01

    The predominant ureolytic bacteria in the pig large intestine were determined while growing pigs were fed a basal diet or basal diet plus copper sulfate, Aureo SP250, or clinoptilolite. Fecal samples were collected from four pigs fed each diet at 3, 9, and 14 weeks and analyzed for total colony counts and percent ureolytic bacteria. Fecal urease activity, ammonia nitrogen, and identity of the ureolytic bacteria were determined at 14 weeks. Copper sulfate and Aureo SP250 reduced the number of ureolytic organisms, with a marked decrease occurring in the Streptococcus spp., which made up 74% of the ureolytic isolates from the pigs on the basal diet. Other ureolytic species detected at lower concentrations were Staphylococcus spp., Selenomonas ruminantium, Bacteroides multiacidus, and Eubacterium limosum. Copper sulfate also reduced fecal urease activity (P less than 0.10). Fecal ammonia concentrations were not different between pigs fed the various diets. These data suggest that the streptococci are the most numerous ureolytic species in the pig intestinal tract and are significantly reduced by copper sulfate and Aureo SP250; however, only copper sulfate reduced intestinal urease activity. PMID:2823707

  14. Crystal structures of copper(II) chloride, copper(II) bromide, and copper(II) nitrate complexes with pyridine-2-carbaldehyde thiosemicarbazone

    SciTech Connect

    Chumakov, Yu. M.; Tsapkov, V. I.; Jeanneau, E.; Bairac, N. N.; Bocelli, G.; Poirier, D.; Roy, J.; Gulea, A. P.

    2008-09-15

    The crystal structures of chloro-(2-formylpyridinethiosemicarbazono)copper dimethyl sulfoxide solvate (I), bromo-(2-formylpyridinethiosemicarbazono)copper (II), and (2-formylpyridinethiosemicarbazono)copper(II) nitrate dimethyl sulfoxide solvate (III) are determined using X-ray diffraction. In the crystals, complexes I and II form centrosymmetric dimers in which the thiosemicarbazone sulfur atom serves as a bridge and occupies the fifth coordination site of the copper atom of the neighboring complex related to the initial complex through the center of symmetry. In both cases, the coordination polyhedron of the complexing ion is a distorted tetragonal bipyramid. Complex III in the crystal structure forms polymer chains in which the copper atom of one complex forms the coordination bond with the thicarbamide nitrogen atom of the neighboring complex. In this structure, the coordination polyhedron of the central atom is an elongated tetragonal bipyramid. It is established that complexes I-III at a concentration of 10{sup -5} mol/l selectively inhibit the growth of 60 to 90 percent of the cancer tumor cells of the human myeloid leukemia (HL-60).

  15. Crystal structures of copper(II) chloride, copper(II) bromide, and copper(II) nitrate complexes with pyridine-2-carbaldehyde thiosemicarbazone

    NASA Astrophysics Data System (ADS)

    Chumakov, Yu. M.; Tsapkov, V. I.; Jeanneau, E.; Bairac, N. N.; Bocelli, G.; Poirier, D.; Roy, J.; Gulea, A. P.

    2008-09-01

    The crystal structures of chloro-(2-formylpyridinethiosemicarbazono)copper dimethyl sulfoxide solvate ( I), bromo-(2-formylpyridinethiosemicarbazono)copper ( II), and (2-formylpyridinethiosemicarbazono)copper(II) nitrate dimethyl sulfoxide solvate ( III) are determined using X-ray diffraction. In the crystals, complexes I and II form centrosymmetric dimers in which the thiosemicarbazone sulfur atom serves as a bridge and occupies the fifth coordination site of the copper atom of the neighboring complex related to the initial complex through the center of symmetry. In both cases, the coordination polyhedron of the complexing ion is a distorted tetragonal bipyramid. Complex III in the crystal structure forms polymer chains in which the copper atom of one complex forms the coordination bond with the thicarbamide nitrogen atom of the neighboring complex. In this structure, the coordination polyhedron of the central atom is an elongated tetragonal bipyramid. It is established that complexes I III at a concentration of 10-5 mol/l selectively inhibit the growth of 60 to 90 percent of the cancer tumor cells of the human myeloid leukemia (HL-60).

  16. Practical method to make a discrete memristor based on the aqueous solution of copper sulfate

    NASA Astrophysics Data System (ADS)

    Merrikh-Bayat, Farshad; Parvizi, Meysam

    2016-06-01

    A new method to realize a discrete memristor is proposed. The device under study consists of a tube filled of aqueous saturated solution of copper sulfate which can be electrolyzed by using two asymmetric copper electrodes, one of which has a considerably smaller cross-sectional area than to the other one. It is shown both theoretically and experimentally that this device has exactly the properties of a memristor if it is designed such that the electrical field and the current density on the thinner electrode when it acts as anode are sufficiently large. Different aspects of the proposed discrete memristor, including pinched hysteresis loop, on-off resistance ratio and memory volatilization, are studied and experimental results are presented.

  17. Effects of low molecular weight chondroitin sulfate on type II collagen-induced arthritis in DBA/1J mice.

    PubMed

    Cho, So Yean; Sim, Joon-Soo; Jeong, Choon Sik; Chang, Seung Yeup; Choi, Don Woong; Toida, Toshihiko; Kim, Yeong Shik

    2004-01-01

    In order to evaluate the improvement in the treatment of chronic arthritis, we investigated chondroitin sulfate depolymerization product (low molecular weight chondroitin sulfate, LMWCS) and intact chondroitin sulfate (CS) in vitro and in vivo. LMWCS was prepared by a chemical depolymerization process induced by hydrogen peroxide in the presence of copper salts. LMWCS (300 mg/kg) and CS (1200 mg/kg) were orally administered to DBA/1J mice once daily for 14 d prior to initial immunization with type II collagen. Their elastase activities and the production of cytokines in sera were examined on type II collagen-induced arthritis in DBA/1J mice. We also compared the paracellular transport of LMWCS and CS across Caco-2 cell monolayers and examined the inhibitory effects on elastase activities. LMWCS inhibited elastase activity slightly, but CS did not show inhibition. Hind paw edema was significantly decreased by LMWCS treatment. Levels of anti-type II collagen antibody and tumor necrosis factor-alpha (TNF-alpha) in sera were also reduced by LMWCS treatment but not in case of CS, although no significant difference was observed between LMWCS and CS on interleukin-6 (IL-6) induction. The LMWCS preparation showed preventive effects on the type II collagen-induced arthritis in DBA/1J mice and better permeability through Caco-2 cells. PMID:14709897

  18. The amyloid precursor protein of Alzheimer's disease in the reduction of copper(II) to copper(I)

    PubMed

    Multhaup, G; Schlicksupp, A; Hesse, L; Beher, D; Ruppert, T; Masters, C L; Beyreuther, K

    1996-03-01

    The transition metal ion copper(II) has a critical role in chronic neurologic diseases. The amyloid precursor protein (APP) of Alzheimer's disease or a synthetic peptide representing its copper-binding site reduced bound copper(II) to copper(I). This copper ion-mediated redox reaction led to disulfide bond formation in APP, which indicated that free sulfhydryl groups of APP were involved. Neither superoxide nor hydrogen peroxide had an effect on the kinetics of copper(II) reduction. The reduction of copper(II) to copper(I) by APP involves an electron-transfer reaction and could enhance the production of hydroxyl radicals, which could then attack nearby sites. Thus, copper-mediated toxicity may contribute to neurodegeneration in Alzheimer's disease. PMID:8596911

  19. Copper(II)-catalyzed reactions of activated aromatics.

    PubMed

    Puzari, A; Baruah, J B

    2000-04-21

    The catalytic reaction of cis-bisglycinato copper(II) monohydrate in the presence of hydrogen peroxide leads to hydroxylation of phenol to give catechol and hydroquinone (1:1.2 ratio) in good yield. 2,6-Dimethylphenol can be hydroxylated by hydrogen peroxide and a catalytic amount of cis-bisglycinato copper(II) monohydrate to give an aggregate of 1,4-dihydroxy-2,6-dimethylbenzene and 2,6-dimethylphenol. A similar reaction of o-cresol gives 2,5-dihydroxytoluene. The reactivity of cis-bisglycinato copper(II) monohydrate in hydrogen peroxide with o-cresol is 4.5 times faster than that of a similar reaction by trans-bisglycinato copper(II) monohydrate. A catalytic reaction of cis-bisglycinato copper(II) monohydrate with aniline in aqueous hydrogen peroxide gives polyanilines in the form of pernigraniline with different amounts of Cu(OH)2 attached to them. The two major components of polyanilines obtained have Mn values of 1040 and 1500, respectively. Resistance of films of these polyanilines increases with temperatures from 40 degrees C to a maximum value at 103 degrees C and then decreases in the region of 103-150 degrees C, showing the property of a thermolectric switch. The aggregate prepared from hydroxylation of 2,6-dimethylphenol shows a similar property in the region of 30-180 degrees C. PMID:10789445

  20. Histopathological and bacterial study of Persian sturgeon fry, Acipenser persicus (Borodin, 1897) exposed to copper sulfate and potassium permanganate.

    PubMed

    Moshtaghi, Batol; Khara, Hossein; Pazhan, Zabiyollah; Shenavar, Alireza

    2016-09-01

    Persian sturgeon frys were exposed to different concentrations of copper sulfate and potassium permanganate in order to the evaluation of their impacts on bacterial load of skin, gill and surrounding water and also the histopathological alternations of gill tissue. For this purpose, the sublethal doses were determined after a pre-test and then the experiment was done in 4 (for copper sulfate: 0.07, 0.14, 026 and 0.5 mg/l) and 5 (for potassium permanganate: 0.07, 0.14, 026, 0.5 and 1 mg/l) treatments with three replicates inside the glass aquaria. Also, one group without disinfecting drug was considered as control for each experiment. The microbial and histopathological investigations were done after 96 h exposure. According to our results, a range of histopathological alternations were observed in gills tissue including mucus coagulation and secretion, hyperplasia, lamellar necrosis, hyperplasia, lamellar adhesion, haemorrhage, thickening of secondary lamellae, hypertrophy of supporter cartilage, clubbing of gill lamellae and sliming of primary lamellae. The severity of these alternations increased with increasing of the doses of the copper sulfate and potassium permanganate. The bacterial load (CFU/g) of gill, skin and surrounding water was lower in 0.07 mg/l copper sulfate treatment and 1 mg/l potassium permanganate treatment (P < 0.05) than in other treatments. In conclusion, our results showed that the certain doses of the copper sulfate and potassium permanganate have disinfecting effects on bacterial load of gill, skin and surrounding water, although this is along with some histopathological alternations. Also, it seems that the copper sulfate has higher disinfecting power than potassium permanganate. PMID:27605784

  1. The Thermal Decomposition of Basic Copper(II) Sulfate.

    ERIC Educational Resources Information Center

    Tanaka, Haruhiko; Koga, Nobuyoshi

    1990-01-01

    Discussed is the preparation of synthetic brochantite from solution and a thermogravimetric-differential thermal analysis study of the thermal decomposition of this compound. Other analyses included are chemical analysis and IR spectroscopy. Experimental procedures and results are presented. (CW)

  2. Copper(I) and copper(II) uranyl heterometallic hybrid materials.

    PubMed

    Weng, Zhehui; Zhang, Zhi-hui; Olds, Travis; Sterniczuk, Marcin; Burns, Peter C

    2014-08-01

    Two copper-uranium heterometallic compounds, [(UO2)3Cu(II)O2(C6NO2)5] (1) and [(UO2)Cu(I)(C6NO2)3] (2), have been synthesized by the reaction of uranyl acetate with copper salts in the presence of isonicotinic acid. Both compounds have been characterized by single-crystal X-ray diffraction, IR, Raman, and UV-vis spectroscopy. In compound 1, interactions between copper and uranium centers occur and result in a three-dimensional pillar layered structure. Compound 1 is also the first example of a heterometallic uranyl organic framework with a trinuclear U3O18 building block. Compound 2 is the first uranyl organic framework that contains monovalent copper, which arises from the reaction of Cu(II) chloride and is assumed to be due to the oxidation of chloride at low pH. PMID:25029287

  3. Oligosaccharides derived from bovine articular cartilage keratan sulfates after keratanase II digestion: implications for keratan sulfate structural fingerprinting.

    PubMed

    Brown, G M; Huckerby, T N; Morris, H G; Abram, B L; Nieduszynski, I A

    1994-04-26

    Keratan sulfate chains were isolated from bovine articular cartilage (6-8-year-old animals) and digested with keratanase II, an endo-beta-N-acetylglucosaminidase [Nakazawa, K., Ito, M., Yamagata, T., & Suzuki, S. (1989) in Keratan Sulphate: Chemistry, Biology and Chemical Pathology (Greiling, H., & Scott, J. E., Eds.) pp 99-110, The Biochemical Society, London]. Twenty-five borohydride-reduced oligosaccharides were purified chromatographically and characterized by one- and two-dimensional NMR spectroscopy. From the structures of these oligosaccharides the following conclusions can be drawn about the mode of action of keratanase II: (1) The enzyme cleaves the beta (1-->3)-glycosidic bond between 6-O-sulfated N-acetyl-glucosamine and galactose, the major products being mono- and disulfated disaccharides. (2) Larger oligosaccharides containing keratanase II susceptible bonds are produced which are resistant to further degradation, e.g., tetrasaccharides from the sulfated poly(N-acetyllactosamine) repeat sequence, fucose-containing penta- and hexasaccharides, and hexa- and heptasaccharides from the linkage region. (3) The enzyme cleaves the beta (1-->3)-glycosidic bond of a fucosylated 6-O-sulfated N-acetylglucosamine. (4) Sialic acid-containing capping fragments are always recovered as pentasaccharides, despite the presence of an apparently susceptible bond. Two new elements of skeletal keratan sulfate structure, namely, the highly sulfated cap NeuAc alpha 2-3Gal(6S) beta 1-4GlcNAc (6S) beta 1-3Gal(6S) beta 1-4GlcNAc (6S)-ol and the difucosylated sequence Gal beta 1-4(Fuc alpha 1-3)GlcNAc(6S)beta 1-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc(6S)-ol, have been identified. A structural model for articular cartilage keratan sulfate is proposed. The potential of the enzyme keratanase II for the structural fingerprinting of subnanogram quantities both of keratan sulfates and of sulfated oligosaccharide selectin ligands is discussed. PMID:8161543

  4. Copper(II)-mediated oxidative transformation of vic-dioxime to furoxan: evidence for a copper(II)-dinitrosoalkene intermediate.

    PubMed

    Das, Oindrila; Paria, Sayantan; Zangrando, Ennio; Paine, Tapan Kanti

    2011-11-21

    The mononuclear copper(II) complex [Cu(H(2)L(1))(2)(H(2)O)](ClO(4))(2) (1) (where H(2)L(1) = 1,10-phenanthroline-5,6-dioxime) reacts with copper(II) perchlorate in acetonitrile at ambient conditions in the presence of triethylamine to afford a copper(II) complex, [Cu(L(3))(2)(H(2)O)](ClO(4))(2) (2a), of 1,10-phenanthroline furoxan. A similar complex [Cu(L(3))(2)Cl](ClO(4)) (2) is isolated from the reaction of H(2)L(1) with copper(II) chloride, triethylamine, and sodium perchlorate in acetonitrile. The two-electron oxidation of the vic-dioxime to furoxan is confirmed from the X-ray single crystal structure of 2. An intermediate species, showing an absorption band at 608 nm, is observed at -20 °C during the conversion of 1 to 2a. A similar blue intermediate is formed during the reaction of [Cu(HDMG)(2)] (H(2)DMG = dimethylglyoxime) with ceric ammonium nitrate, but H(2)DMG treated with ceric ammonium nitrate does not form any intermediate. This suggests the involvement of a copper(II) complex in the intermediate step. The intermediate species is also observed during the two-electron oxidation of other vic-dioximes. On the basis of the spectroscopic evidence and the nature of the final products, the intermediate is proposed to be a mononuclear copper(II) complex ligated by a vic-dioxime and a dinitrosoalkene. The dinitrosoalkene is generated upon two-electron oxidation of the dioxime. The transient blue color of the dioxime-copper(II)-dinitrosoalkene complex may be attributed to the ligand-to-ligand charge transfer transition. The intermediate species slowly decays to the corresponding two-electron oxidized form of vic-dioxime, i.e. furoxan and [Cu(CH(3)CN)(4)](ClO(4)). The formation of two isomeric furoxans derived from the reaction of an asymmetric vic-dioxime, hexane-2,3-dioxime, and copper(II) perchlorate supports the involvement of a dinitrosoalkene species in the intermediate step. In addition, the oxidation of 2,9-dimethyl-1,10-phenanthroline-5,6-dioxime (H(2)L

  5. Copper avoidance and mortality of juvenile brown trout (Salmo trutta) in tests with copper-sulfate-treated water from West Branch Reservoir, Putnam County, New York

    USGS Publications Warehouse

    Baldigo, Barry P.; Baudanza, T.P.

    2001-01-01

    Copper-avoidance tests and acute-toxicity (mortality) tests on hatchery-reared, young-of- the-year brown trout (salmo trutta) were conducted with water from West Branch Reservoir to assess the avoidance response to copper sulfate treatment, which is used occasionally by New York City Department of Environmental Protection to decrease phytoplankton populations in the reservoir. Avoidance-test results indicate that juvenile brown trout tend to avoid dissolved copper concentrations greater than about 55 ?g/L (micrograms per liter), which is the approximate avoidance-response threshold. The mean net avoidance response of brown trout to dissolved copper concentrations of 70 and 100 ?g/L, and possibly 80 ?g/L, was significantly different (at a = 0.1) from the mean net avoidance response of fish to control (untreated) water and to treated water at most other tested concentrations. Mortality-test results indicate that the 96-hr median lethal concentration (LC50) of dissolved copper was 61.5 ?g/L. All (100 percent) of the brown trout died at a dissolved copper concentration of 85 ?g/L, many died at concentrations of 62 ?g/L and 70 ?g/L, and none died in the control waters (7 ?g/L) or at concentrations of 10, 20, or 45 ?g/L. The estimated concentration of dissolved copper that caused fish mortality (threshold) was 53.5 ?g/L, virtually equivalent to the avoidance-response threshold. Additional factors that could affect the copper-avoidance and mortality response of individual brown trout and their populations in West Branch Reservoir include seasonal variations in certain water-quality parameters, copper-treatment regimes, natural fish distributions during treatment, and increased tolerance due to acclimation. These warrant additional study before the findings from this study can be used to predict the effects that copper sulfate treatments have on resident fish populations in New York City reservoirs.

  6. Dose-confirmation of copper sulfate for treating fungus on channel catfish eggs at a commercial hatchery

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study at a commercial hatchery was required by the U.S. Food and Drug Administration to provide independent substantiation of the results of a previous laboratory dose confirmation study to control fungus (Saprolegnia spp.) on channel catfish Ictalurus punctatus eggs with copper sulfate (CuSO4)...

  7. Optimizing copper sulfate treatments for fungus control on channel catfish eggs in high alkalinity/moderate hardness water

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Interest in the use of copper sulfate (CuSO4) for fungus (Saprolegnia sp.) control in catfish hatcheries has developed over the past few years. A range-finding study was designed to determine the optimum concentration needed for fungus control on channel catfish eggs in 23.5°C well water at the Stu...

  8. Comparative effects of copper sulfate or potassium permanganate on channel catfish concurrently infected with Flavobacterium columnare and Ichthyobodo necator

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An opportunistic study was conducted to determine the effects of two chemical therapeutants on channel catfish (CCF) Ictalurus punctatus concurrently infected Flavobacterium columnare and Ichthyobodo necator. Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were investigated for their abil...

  9. The Synthesis of Copper(II) Carboxylates Revisited

    ERIC Educational Resources Information Center

    Kushner, Kevin; Spangler, Robert E.; Salazar, Ralph A., Jr.; Lagowski, J. J.

    2006-01-01

    An electrochemical synthesis of copper(II) carboxylates has been developed and used in the general chemistry laboratory course for chemistry majors. This synthesis, using nonaqueous solutions, supplements the strategy of providing experiences in synthetic chemistry described by Yoder et al. ("J. Chem. Educ." 1995, 72, 267). (Contains 1 table.)

  10. Sub-lethal effects of a copper sulfate fungicide on development and reproduction in three coccinellid species.

    PubMed Central

    Michaud, J.P.; Angela K., Grant

    2003-01-01

    Copper-based fungicides reliably control various foliar diseases in citrus production, although they are suspected to exacerbate mite problems through various mechanisms. Studies have shown negative effects of various copper formulations on entomopathogenic fungi, nematodes, and parasitoids, but few have sought to measure its impact on the biology of predatory insects. We exposed the larvae of three species of ladybeetle (Coleoptera: Coccinellidae) to field rates of copper sulfate in combination with petroleum oil, a formulation commonly applied in Florida citrus. First instar larvae of Curinus coeruleus Mulsant, Harmonia axyridis Pallas, and Olla v-nigrum Mulsant received a 24 h exposure to residues on Petri dishes, and another 24 h exposure in the third instar. Treated larvae of all three species survived to adulthood at the same rate as control larvae, but larvae of O. v-nigrum experienced a significant increase in developmental time. Female adults of C. coeruleus and H. axyridis receiving copper sulfate exposures as larvae did not differ from control adults in pre-reproductive period, fecundity or fertility over ten days of reproduction. Treated O. v-nigrum females had significantly longer pre-reproductive periods than control females and laid significantly fewer eggs, although egg fertility was equivalent. We conclude that copper-sulfate fungicides are unlikely to disrupt biological control processes in citrus groves that are mediated by these coccinellid beetles. PMID:15841232

  11. Thermal Decomposition of Copper (II) Calcium (II) Formate

    NASA Astrophysics Data System (ADS)

    Leyva, A. G.; Polla, G.; de Perazzo, P. K.; Lanza, H.; de Benyacar, M. A. R.

    1996-05-01

    The presence of different stages in the thermal decomposition process of CuCa(HCOO) 4has been established by means of TGA at different heating rates, X-ray powder diffraction of quenched samples, and DSC methods. During the first stage, decomposition of one of the two copper formate structural units contained in the unit cell takes place. The presence of CuCa 2(HCOO) 6has been detected. Calcium formate structural units break down at higher temperatures; the last decomposition peak corresponds to the appearance of different calcium-copper oxides.

  12. Phosphate effects on copper(II) and lead(II) sorption to ferrihydrite

    NASA Astrophysics Data System (ADS)

    Tiberg, Charlotta; Sjöstedt, Carin; Persson, Ingmar; Gustafsson, Jon Petter

    2013-11-01

    Transport of lead(II) and copper(II) ions in soil is affected by the soil phosphorus status. Part of the explanation may be that phosphate increases the adsorption of copper(II) and lead(II) to iron (hydr)oxides in soil, but the details of these interactions are poorly known. Knowledge about such mechanisms is important, for example, in risk assessments of contaminated sites and development of remediation methods. We used a combination of batch experiments, extended X-ray absorption fine structure (EXAFS) spectroscopy and surface complexation modeling with the three-plane CD-MUSIC model to study the effect of phosphate on sorption of copper(II) and lead(II) to ferrihydrite. The aim was to identify the surface complexes formed and to derive constants for the surface complexation reactions. In the batch experiments phosphate greatly enhanced the adsorption of copper(II) and lead(II) to ferrihydrite at pH < 6. The largest effects were seen for lead(II).

  13. [Washing copper (II)-contaminated soil using surfactant solutions].

    PubMed

    Zhao, Bao-wei; Wu, Yong-qi; Ma, Chan-Yuan; Zhu, Rui-jia

    2009-10-15

    The batch equilibrium washing of copper (II) in the soil matrix by anionic surfactant, sodium dodecylbenzyl sulfonate (SDBS), nonionic surfactant, octylphenoxypolyethoxyethanol (TX100), and their mixture (SDBS-TX100), was studied and compared. The influences of surfactant concentrations, washing time, pH values of solutions, ratios of soil to water and inorganic salts on washing efficiency were investigated. It was shown that the washing efficiency differed with the kinds of surfactants. Given the initial surfactant concentrations, the washing of copper (II) by single SDBS was greater than those by single TX100 and the mixed SDBS-TX100. The washing efficiency by 6 000 mg x L(-1) of SDBS was up to 46.3%, which was 5.8, 10.8, 10.8 and 19.3 times as those by SDBS-TX100 (3:1), SDBS-TX100 (1:1), SDBS-TX100 (1:3) and single TX100 respectively. When the ratio of soil to water was 1 to 10 and washing time reached 24 h, the washing efficiency achieved the maximum. pH values of solutions had obvious effect on the washing of copper (II). The washing efficiency of copper decreased sharply with the increase of pH. At the high acidity (pH = 1.50), the washing efficiency of copper (II) was up to 95%. The smaller the ratios of soil to water were, the higher the washing efficiencies would be. The existence of inorganic salts with the certain concentrations, such as Na+, Ca2+ and Mg2+, could not influence the washing capacity of surfactants, but the excessive Mg2+ (more than 500 mg x L(-1)) could resulted in the precipitation of SDBS. The results will make an implication for surfactant-enhanced remediation of soils contaminated with heavy metals. PMID:19968132

  14. Two unprecedented 1D coordination polymer chains based on tetranuclear copper(II) building blocks

    SciTech Connect

    Li Gaijuan; Xing Yan Song Shuyan; Xu Ning; Liu Xianchun; Su Zhongmin

    2008-09-15

    The reaction of copper(II) sulfate with pyridine in DMF or methanol yield two unprecedented Cu(II) coordination polymers {l_brace}[Cu{sub 4}({mu}{sub 4}-O)(py){sub 4}(SO{sub 4}){sub 4}][{mu}-Cu(py)(DMF){sub 2}]{r_brace}{sub n}(1) and {l_brace}[Cu{sub 4}({mu}{sub 4}-O)(py){sub 4}(SO{sub 4}){sub 4}][{mu}-Cu(py){sub 4}]{r_brace}{sub n}(2), respectively. Single-crystal X-ray diffraction indicated that compound 1 crystallizes in the monoclinic system, space group p2(1)/n, a=14.542(5) A, b=16.359(5) A, c=18.951(5) A, {beta}=92.047(5){sup o}, V=4505(2) A{sup 3}, Z=4 while 2 is monoclinic C2/c, a=23.078(5) A, b=10.214(5) A, c=23.142(5) A, {beta}=115.471(5){sup o}, V=4925(3) A{sup 3}, Z=4. Both of the two compounds consist of tetrahedral tetranuclear [Cu{sub 4}({mu}{sub 4}-O)(py){sub 4}(SO{sub 4}){sub 4}] clusters that are bridged by pentacoordinated Cu atom for 1 or hexacoordinated Cu atoms for 2 through the sulfate oxygen to form the infinite one-dimensional polymer chains. - Graphical abstract: Two unprecedented Cu(II) coordination polymers have been prepared by using solvothermal method; they consist of tetrahedral tetranuclear clusters that are bridged by unique Cu(II) atom through the sulfate oxygen to form the infinite one-dimensional polymer chains (a) for complex 1 and (b) for complex 2.

  15. Chemical speciation and bioavailability of Cu(II). Study of the ionic copper(II) and bis(glycinate)-copper(II) accumulation by Lemna species

    SciTech Connect

    Benda, F.; Kouba, J. )

    1991-03-01

    In this paper, the authors examined the accumulation of copper(II) in, and its toxic effect on, duckweed, a plant which exhibits extremely high concentration factors. The effect of copper(II) was investigated by adding it to the minimal medium in two forms: CuSO{sub 4} and (Cu(Gly){sub 2}). The neutral (2:1) tetracoordinated bis(glycinate)-copper(II) complex is constituted by two five-membered rings bonded to the central copper atom with the cis configuration. This complex was chosen to model the function of a neutral species (eliminating the charge effect) involving a nontoxic ligand, for which - in contrast to the hydrated Cu{sup 2+} species - direct permeation through the cell wall is conceivable.

  16. Determining the Amount of Copper(II) Ions in a Solution Using a Smartphone

    ERIC Educational Resources Information Center

    Montangero, Marc

    2015-01-01

    When dissolving copper in nitric acid, copper(II) ions produce a blue-colored solution. It is possible to determine the concentration of copper(II) ions, focusing on the hue of the color, using a smartphone camera. A free app can be used to measure the hue of the solution, and with the help of standard copper(II) solutions, one can graph a…

  17. Synthesis, spectral characterization and biological evaluation of copper(II) and nickel(II) complexes with thiosemicarbazones derived from a bidentate Schiff base

    NASA Astrophysics Data System (ADS)

    Chandra, Sulekh; Bargujar, Savita; Nirwal, Rita; Yadav, Neesha

    2013-04-01

    Complexes of copper(II) and nickel(II) of general composition M(L)2X2, have been synthesized with the ligand 1-Tetralone thiosemicarbazone (where L = 1-Tetralone thiosemicarbazone and X=Cl,1/2SO42-). The molar conductance of the complexes in fresh solution of DMSO lies in the range of 10-20 Ω-1 cm2 mol-1 indicating their non-electrolytic behavior. Thus, the complexes may be formulated as [M(L2)X2]. Ligand was characterized by mass, NMR, IR and single crystallographic studies. All the complexes were characterized by elemental analyses, magnetic moments, IR, electronic and EPR spectral studies. The IR spectral data of ligand indicated the involvement of sulfur and azomethine nitrogen in coordination to the central metal ion. The copper(II) and nickel(II) complexes were found to have magnetic moments1.93-1.96 BM and 2.91-2.94 BM corresponding to one and two unpaired electrons respectively. On the basis of molar conductance, EPR, electronic and infrared spectral studies, a tetragonal geometry has been assigned for Cu(II) chloride complex and trigonal bipyramidal to Cu(II) sulfate complex but an octahedral geometry for Ni(II) complexes. Newly synthesized ligand and its Cu(II) and Ni(II) complexes have also been screened against different bacterial and fungal species.

  18. Thermal Decomposition of Copper (II) Dicalcium (II) Formate

    NASA Astrophysics Data System (ADS)

    de Perazzo, P. K.; Leyva, A. G.; Polla, G.; Parisi, F.; de Benyacar, M. A. R.; Smichowski, P.; Lanza, H.

    1997-09-01

    The unit cell obtained through X-ray single crystal analysis of the synthetized CuCa 2(HCOO) 6crystals corresponds to a supercell of the basic structure described by M. Sanchis et al.( Inorg. Chem.31, 2915 (1992)). Thermal decomposition of this sample shows two stages up to 300°C; the first can be related to the superstructure, and the second corresponds to the breaking down of the remaining copper formate structural units and the simultaneous decomposition of the sample.

  19. 63Cu NQR in copper (II) compounds

    NASA Astrophysics Data System (ADS)

    Bastow, T. J.; Campbell, I. D.; Whitfield, H. J.

    1980-01-01

    We report observations of 63Cu NQR in CuF 2, KCuF 3, and RbCuF 3 in the paramagnetic state, NQR line widths of 63Cu in CuF 2 and CuBr 2 and of 81Br in CuBr 2, SnBr 2 and ZnBr 2. The NQR resonances of certain Cu (II) paramagnetic compounds are exchange-narrowed to values commensurate with linewidths of the diamagnetic infinite-lattice compounds.

  20. Structure of catabolite activator protein with cobalt(II) and sulfate

    SciTech Connect

    Rao, Ramya R.; Lawson, Catherine L.

    2014-04-15

    The crystal structure of E. coli catabolite activator protein with bound cobalt(II) and sulfate ions at 1.97 Å resolution is reported. The crystal structure of cyclic AMP–catabolite activator protein (CAP) from Escherichia coli containing cobalt(II) chloride and ammonium sulfate is reported at 1.97 Å resolution. Each of the two CAP subunits in the asymmetric unit binds one cobalt(II) ion, in each case coordinated by N-terminal domain residues His19, His21 and Glu96 plus an additional acidic residue contributed via a crystal contact. The three identified N-terminal domain cobalt-binding residues are part of a region of CAP that is important for transcription activation at class II CAP-dependent promoters. Sulfate anions mediate additional crystal lattice contacts and occupy sites corresponding to DNA backbone phosphate positions in CAP–DNA complex structures.

  1. Responses of phtyoplankton photosynthesis and phosphorus kinetics to resuspended sediments in copper sulfate-treated ponds

    SciTech Connect

    Nalewajko, C.; Prepas, E.E.

    1996-01-01

    Six farm ponds (dugouts) and one lake that differ in the history of copper sulfate (CuSO{sub 4}) treatment were selected for studies of effects of sediments resuspension on phytoplankton. All sites are located within 50 km of Peace River, Alberta, and are shallow, hardwater, and eutrophic. Effects of sediment resuspension on phytoplankton photosynthesis were assessed by changes in the photosynthesis-irradiance P-D curve parameters, Pmax and {alpha}, after addition of sediment at 2% v/v to lakewater samples; the effects on phytoplankton P-state were assessed by changes in {sup 32}PO{sub 4} turnover time. Copper concentrations in sediments of Gour No. 4, the dugout that had received the largest dosage of CuSO{sub 4}, were 60-times greater than untreated sites but were only 1.5 to 3-times greater at the other treated sites. Changes of Pmax and {alpha} were not correlated with Cu concentrations in the sediments. Instead, the prevailing P-state in lakewater could better explain the observed trends in Pmax after sediment addition. Pmax values decreased at sites where phytoplankton were P-limited ({sup 32} P-PO{sub 4} turnover time <63 min) and increased at more P-sufficient sites ({sup 32}P-PO{sub 4} turnover time >63 min). Stimulation of Pmax and increase in {sup 32}P-PO{sub 4} turnover time were positively correlated. With the exception of Gour No. 4, values of a increased in all treatments. Similar changes in Pmax and a in response to sediment addition occurred in laboratory experiments with P-sufficient cultures of Anabaena flos-aquae. We suggest that, with the exception of grossly Cu-polluted sediments, resuspension of sediments in waters previously treated with CuSO{sub 4} will enhance phytoplankton photosynthesis by increasing P availability, and possibly by supplying Cu at trace metal levels. 25 refs., 4 figs., 6 tabs.

  2. Characterization of the Copper(II) Binding Sites in Human Carbonic Anhydrase II

    PubMed Central

    Nettles, Whitnee L.; Song, He; Farquhar, Erik R.; Fitzkee, Nicholas C.; Emerson, Joseph P.

    2015-01-01

    Human carbonic anhydrase (CA) is a well-studied, robust, mononuclear Zn-containing metalloprotein that serves as an excellent biological ligand system to study the thermodynamics associated with metal ion coordination chemistry in aqueous solution. The apo-form of human carbonic anhydrase II (CA) binds two equivalents of copper(II) with high affinity. The Cu2+ ions bind independently forming two non-coupled type-II copper centers in CA (CuA and CuB). However, the location and coordination mode of the CuA site in solution is unclear, compared to the CuB site that has been well characterized. Using paramagnetic NMR techniques and X-ray absorption spectroscopy we have identified an N-terminal Cu2+ binding location and collected information on the coordination mode of the CuA site in CA, which is consistent with a four to five coordinate N-terminal Cu2+ binding site reminiscent to a number of N-terminal copper(II) binding sites including the copper(II)-ATCUN and copper(II)-beta-amyloid complexes. Additionally, we report a more detailed analysis of the thermodynamics associated with copper(II) binding to CA. Although we are still unable to fully deconvolute Cu2+ binding data to the high-affinity CuA site, we have derived pH- and buffer-independent values for the thermodynamics parameters K and ΔH associated with Cu2+ binding to the CuB site of CA to be 2 × 109 and −17.4 kcal/mol, respectively. PMID:26010488

  3. Interaction of Fe(II) with phosphate and sulfate on iron oxide surfaces

    NASA Astrophysics Data System (ADS)

    Hinkle, Margaret A. G.; Wang, Zimeng; Giammar, Daniel E.; Catalano, Jeffrey G.

    2015-06-01

    Sulfate and phosphate, oxoanions common in natural systems, affect iron oxide growth and dissolution processes, the adsorption behavior of divalent cations, and iron oxide phase transformations. These oxoanions may thus influence Fe(II) adsorption behavior and subsequently alter the mechanisms and products of Fe(II)-catalyzed Fe(III) oxide recrystallization processes, such as trace metal repartitioning. In this study, the macroscopic and molecular-scale effects of the coadsorption of Fe(II) and sulfate or phosphate onto Fe(III) oxide surfaces were investigated. Macroscopic adsorption edges show that both sulfate and phosphate increase Fe(II) adsorption and that Fe(II) increases sulfate and phosphate adsorption. Attenuated total reflectance Fourier transform infrared spectroscopy shows that the cooperative adsorption behavior of oxoanions and aqueous Fe(II) likely results from a combination of ternary complexation and electrostatic interactions. Surface complexation modeling requires the inclusion of ternary complexes to simulate all conditions of the macroscopic data, further suggesting that these oxoanions and Fe(II) form ternary complexes on Fe(III) oxide surfaces. Despite clear evidence in previous research for Fe(II) oxidation upon adsorption on iron oxide surfaces, this work shows that Fe(II) also displays macroscopic and molecular-scale behaviors associated with divalent (i.e., non-oxidative) cation adsorption. Prior work has shown that metal release from iron oxides caused by ET-AE reactions is directly proportional to the macroscopically-determined Fe(II) surface coverage. Predicting the effects of sulfate and phosphate on processes controlled by ET-AE reactions at redox interfaces, such as mineral phase transformations and trace element repartitioning, may thus not require the explicit consideration of electron transfer processes.

  4. Copper sulfate improves pullulan production by bioconversion using whole cells of Aureobasidium pullulans as the catalyst.

    PubMed

    Wang, Dahui; Ju, Xiaomin; Zhang, Gaochuan; Wang, Donghua; Wei, Gongyuan

    2016-10-01

    The effects of mineral salts on pullulan production by bioconversion using whole cells of Aureobasidium pullulans CCTCC M 2012259 as the catalyst were investigated. Copper sulfate (CuSO4) improved pullulan production by 36.2% and 42.3% when added at the optimum concentration of 0.2mg/L to the bioconversion broth or seed medium, respectively, as compared with controls without CuSO4 addition. Pullulan production was further enhanced when CuSO4 was added to both seed medium and bioconversion broth simultaneously. In order to probe the mechanism of CuSO4 improvement, cell viability, membrane integrity, intracellular adenosine triphosphate (ATP) levels and the activities of key enzymes involved in pullulan biosynthesis were determined. As a result, CuSO4 increased the activities of key biosynthetic enzymes, maintained intracellular ATP at a higher level, and accelerated the rate of pullulan secretion, all of which contributed to improved pullulan production by bioconversion. PMID:27312631

  5. Effects of various polyoxyethylene sorbitan monooils (Tweens) and sodium dodecyl sulfate on reflux synthesis of copper nanoparticles

    SciTech Connect

    Zhang Xifeng; Yin Hengbo . E-mail: yin@ujs.edu.cn; Cheng Xiaonong; Hu Huifeng; Yu Qi; Wang Aili

    2006-11-09

    Size-controlled synthesis of phase pure Cu nanoparticles was carried out by using copper sulfate pentahydrate as a precursor, ascorbic acid as a reductant, Tweens and sodium dodecyl sulfate (SDS) as modifiers in an aqueous solution at 80 deg. C. The as-prepared Cu nanoparticles were characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and Fourier transform infrared (FT-IR). The stabilizing effects of SDS and Tweens on the Cu nanoparticles should be through the coordination between Cu nanoparticles and the respective sulfate group and oxygen-containing bond. The synergic effect of the composite SDS and Tweens on Cu nanoparticles was different from those arising from the individuals.

  6. Anodonta imbecillis copper sulfate reference toxicant/food test, Clinch River - Environmental Restoration Program (CR-ERP)

    SciTech Connect

    Simbeck, D.J.

    1997-06-01

    Reference toxicant testing using juvenile freshwater mussels was conducted as part of the CR-ERP biomonitoring study of Clinch River sediments to assess the sensitivity of test organisms and the overall performance of the test. Tests were conducted using moderately hard synthetic water spiked with known concentrations of copper as copper sulfate. Two different foods, phytoplankton and YCT-Selenastrum (YCT-S), were tested in side by side tests to compare food quality. Toxicity testing of copper sulfate reference toxicant was conducted from July 6-15, 1993. The organisms used for testing were juvenile fresh-water mussels (Anodonta imbecillis). Results from this test showed LC{sub 50} values of 0.97 and 0.84 mg Cu/L for phytoplankton and YCT-S, respectively. Previously obtained values for phytoplankton tests are 2.02 and 1.12 mg Cu/L. Too few tests have been conducted with copper as the toxicant to determine a normal range of values. Although significant reduction in growth, compared to the phytoplankton control, was seen in all treatments, including the YCT-S Control, the consequence of this observation has not been established. Ninety-day testing of juvenile mussels exhibited large variations in growth within treatment and replicate groups.

  7. Desorption measurements of copper and copper alloys for PEP-II

    SciTech Connect

    Foerster, C.L.; Halama, H.; Korn, G.; Calderon, M.; Barletta, W.

    1992-12-31

    PEP-II will be a meson factory circulating asymmetric beams of 9 GeV and 3.1 GeV having maximum currents of 3.0A. Copper beam chambers and absorbers will intercept resulting synchrotron radiation and it is critical that the dynamic gas load from photo stimulated desorption (PSD) and thermal outgassing is below 2 {times} 10{sup {minus}1} molecules per photon. An experiment was set up to measure PSD from 1m long bar samples and a chamber sample, fabricated from selected copper and copper alloys then exposed to white light with a critical energy of 500 eV, on beamline U10B at the VUV ring of the National Synchrotron Light Source (NSLS). Based on U10B results a prototype chamber was built and will be exposed to white light with higher critical energies of up to 5 KeV, on beamline X-28A at the X-ray Ring of the NSLS. This paper presents the measurements of H{sub 2}, CO, CO{sub 2} and CH{sub 4} desorption yields as function of accumulated photon flux, angle of incidence, sample material, and surface condition. The results are compared with that of previous work on similar materials and with that of others for copper.

  8. Genome Sequence of Desulfovibrio sp. A2, a Highly Copper Resistant, Sulfate-Reducing Bacterium Isolated from Effluents of a Zinc Smelter at the Urals

    PubMed Central

    Mancini, Stefano; Abicht, Helge K.; Karnachuk, Olga V.; Solioz, Marc

    2011-01-01

    Desulfovibrio sp. A2 is an anaerobic Gram-negative sulfate-reducing bacterium with remarkable tolerance to copper. It was isolated from wastewater effluents of a zinc smelter at the Urals. Here, we report the 4.2-Mb draft genome sequence of Desulfovibrio sp. A2 and identify potential copper resistance mechanisms. PMID:22072648

  9. Modeling of copper(II) and zinc(II) extraction from chloride media with Kelex 100

    SciTech Connect

    Bogacki, M.B.; Zhivkova, S.; Kyuchoukov, G.; Szymanowski, J.

    2000-03-01

    The extraction of copper(II) and zinc(II) from acidic chloride solutions with protonated Kelex 100 (HL) was studied and the extraction isotherms were determined for systems containing individual metal ions and their mixtures. A chemical model was proposed and verified. It considers the coextraction of the following species: MCl{sub 4}(H{sub 2}L){sub 2}, MCl{sub 4}(H{sub 2}L){sub 2}{center_dot}HCl, MCl{sub 3}(H{sub 2}L), ML{sub 2}, and H{sub 2}L{center_dot}HCl. Zinc(II) is extracted as the metal ion pairs, while copper(II) can be extracted as the metal ion pair and the chelate. The model can be used to predict the effect of experimental conditions on extraction and coextraction of the metal ions considered.

  10. Homeopathic Preparations of Quartz, Sulfur and Copper Sulfate Assessed by UV-Spectroscopy

    PubMed Central

    Wolf, Ursula; Wolf, Martin; Heusser, Peter; Thurneysen, André; Baumgartner, Stephan

    2011-01-01

    Homeopathic preparations are used in homeopathy and anthroposophic medicine. Although there is evidence of effectiveness in several clinical studies, including double-blinded randomized controlled trials, their nature and mode of action could not be explained with current scientific approaches yet. Several physical methods have already been applied to investigate homeopathic preparations but it is yet unclear which methods are best suited to identify characteristic physicochemical properties of homeopathic preparations. The aim of this study was to investigate homeopathic preparations with UV-spectroscopy. In a blinded, randomized, controlled experiment homeopathic preparations of copper sulfate (CuSO4; 11c–30c), quartz (SiO2; 10c–30c, i.e., centesimal dilution steps) and sulfur (S; 11×–30×, i.e., decimal dilution steps) and controls (one-time succussed diluent) were investigated using UV-spectroscopy and tested for contamination by inductively coupled plasma mass spectrometry (ICP-MS). The UV transmission for homeopathic preparations of CuSO4 preparations was significantly lower than in controls. The transmission seemed to be also lower for both SiO2 and S, but not significant. The mean effect size (95% confidence interval) was similar for the homeopathic preparations: CuSO4 (pooled data) 0.0544% (0.0260–0.0827%), SiO2 0.0323% (–0.0064% to 0.0710%) and S 0.0281% (–0.0520% to 0.1082%). UV transmission values of homeopathic preparations had a significantly higher variability compared to controls. In none of the samples the concentration of any element analyzed by ICP-MS exceeded 100 ppb. Lower transmission of UV light may indicate that homeopathic preparations are less structured or more dynamic than their succussed pure solvent. PMID:19474239

  11. Aromatic C-nitrosation by a copper(II)-nitrosyl complex.

    PubMed

    Rout, Kanhu Charan; Mondal, Biplab

    2015-01-28

    Copper(II) complex of 4-amino-3-hydroxy-1-sulphonic acid was synthesized and characterized. Upon addition of nitric oxide, the copper(II) center of the complex in methanol was found to undergo reduction through an unstable copper(II)-nitrosyl intermediate. The formation of the intermediate was confirmed by UV-visible and FT-IR spectroscopy. The reduction of the copper(II) center was accompanied with a simultaneous C-nitrosation of the aromatic ring of the ligand. The C-nitrosation product was isolated and characterized by various spectroscopic analyses. PMID:25476484

  12. EXAFS study of mercury(II) sorption to Fe- and Al-(hydr)oxides - II. Effects of chloride and sulfate

    USGS Publications Warehouse

    Kim, C.S.; Rytuba, J.J.; Brown, Gordon E., Jr.

    2004-01-01

    Common complexing ligands such as chloride and sulfate can significantly impact the sorption of Hg(II) to particle surfaces in aqueous environmental systems. To examine the effects of these ligands on Hg(II) sorption to mineral sorbents, macroscopic Hg(II) uptake measurements were conducted at pH 6 and [Hg]i=0.5 mM on goethite (??-FeOOH), ??-alumina (??-Al2O3), and bayerite (??-Al(OH)3) in the presence of chloride or sulfate, and the sorption products were characterized by extended X-ray absorption fine structure (EXAFS) spectroscopy. The presence of chloride resulted in reduced uptake of Hg(II) on all three substrates over the Cl- concentration ([Cl-]) range 10-5 to 10-2 M, lowering Hg surface coverages on goethite, ??-alumina, and bayerite from 0.42 to 0.07 ??mol/m2, 0.06 to 0.006 ??mol/m2, and 0.55 to 0.39 ??mol/m2 ([Cl -]=10-5 to 10-3 M only), respectively. This reduction in Hg(II) uptake is primarily a result of the formation of stable, nonsorbing aqueous HgCl2 complexes in solution, limiting the amount of free Hg(II) available to sorb. At higher [Cl-] beam reduction of Hg(II) to Hg(I) was observed, resulting in the possible formation of aqueous Hg2Cl2 species and the precipitation of calomel, Hg 2Cl2(s). The presence of sulfate caused enhanced Hg(II) uptake over the sulfate concentration ([SO42-]) range 10-5 to 0.9 M, increasing Hg surface coverages on goethite, ??-alumina, and bayerite from 0.39 to 0.45 ??mol/m2, 0.11 to 0.38 ??mol/m2, and 0.36 to 3.33 ??mol/m2, respectively. This effect is likely due to the direct sorption or accumulation of sulfate ions at the substrate interface, effectively reducing the positive surface charge that electrostatically inhibits Hg(II) sorption. Spectroscopic evidence for ternary surface complexation was observed in isolated cases, specifically in the Hg-goethite-sulfate system at high [SO42-] and in the Hg-goethite-chloride system. ?? 2003 Elsevier Inc. All rights reserved.

  13. New Heteroleptic Copper(II) Complexes as MOCVD Precursors

    NASA Astrophysics Data System (ADS)

    Krisyuk, V. V.; Sysoev, S. V.; Rumyantsev, Y. M.; Prokhorova, S. A.; Maximovskiy, E. V.; Kosinova, M. L.; Igumenov, I. K.

    New volatile heteroleptic copper(II) complexes having beta-ketoiminate (O,N) and diketonate (O,O) ligands in one molecule were tested as precursors for LPCVD of copper films. Saturated vapor pressure was measured and compared for new compounds Cu(ki)(hfa) and Cu(dpk)(hfa), where ki = pentane-2-imino-4-onato, hfa = 1,1,1,5,5,5-hexafluoro-pentane- 2,4-dionato, dpk= 2,2,6,6-tetramethyl-3-iminoheptane-5-onato. The precursors are air stable and non hygroscopic compounds with long shelf life. It was demonstrated that copper metal films can be selectively deposited on metallic surfaces in the presence of hydrogen as a gas-reactant at temperatures of 250, 300, 350 °C and pressure of 20 Torr. Si(100), SiO2 (melted quartz), stainless steel, and Cu, Al, RuO2, Ru and Ta sublayers on Si(100) were tested as substrate materials. Deposited films were analyzed and characterized by X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and scanning electron microscopy (SEM).

  14. Static renewal tests using Anodonta imbecillus (freshwater mussels). Anodonta imbecillis copper sulfate reference toxicant test, Clinch River-Environmental Restoration Program (CR-ERP)

    SciTech Connect

    Simbeck, D.J.

    1993-12-31

    Reference toxicant testing using juvenile freshwater mussels was conducted as part of the CR-ERP biomonitoring study of Clinch River sediments to assess the sensitivity of test organisms and the overall performance of the test. Tests were conducted using moderately hard synthetic water spiked with known concentrations of copper as copper sulfate. Toxicity testing of copper sulfate reference toxicant was conducted from May 12--21, 1993. The organisms used for testing were juvenile fresh-water mussels (Anodonta imbecillis). Results from this test showed an LC{sub 50} value of 1.12 mg Cu/L which is lower than the value of 2.02 mg Cu/L obtained in a previous test. Too few tests have been conducted with copper as the toxicant to determine a normal range of values. Attachments to this report include: Toxicity test bench sheets and statistical analyses; Copper analysis request and results; and Personnel training documentation.

  15. Efficient electron-induced removal of oxalate ions and formation of copper nanoparticles from copper(II) oxalate precursor layers

    PubMed Central

    Rückriem, Kai; Grotheer, Sarah; Vieker, Henning; Penner, Paul; Beyer, André; Gölzhäuser, Armin

    2016-01-01

    Summary Copper(II) oxalate grown on carboxy-terminated self-assembled monolayers (SAM) using a step-by-step approach was used as precursor for the electron-induced synthesis of surface-supported copper nanoparticles. The precursor material was deposited by dipping the surfaces alternately in ethanolic solutions of copper(II) acetate and oxalic acid with intermediate thorough rinsing steps. The deposition of copper(II) oxalate and the efficient electron-induced removal of the oxalate ions was monitored by reflection absorption infrared spectroscopy (RAIRS). Helium ion microscopy (HIM) reveals the formation of spherical nanoparticles with well-defined size and X-ray photoelectron spectroscopy (XPS) confirms their metallic nature. Continued irradiation after depletion of oxalate does not lead to further particle growth giving evidence that nanoparticle formation is primarily controlled by the available amount of precursor. PMID:27547602

  16. Efficient electron-induced removal of oxalate ions and formation of copper nanoparticles from copper(II) oxalate precursor layers.

    PubMed

    Rückriem, Kai; Grotheer, Sarah; Vieker, Henning; Penner, Paul; Beyer, André; Gölzhäuser, Armin; Swiderek, Petra

    2016-01-01

    Copper(II) oxalate grown on carboxy-terminated self-assembled monolayers (SAM) using a step-by-step approach was used as precursor for the electron-induced synthesis of surface-supported copper nanoparticles. The precursor material was deposited by dipping the surfaces alternately in ethanolic solutions of copper(II) acetate and oxalic acid with intermediate thorough rinsing steps. The deposition of copper(II) oxalate and the efficient electron-induced removal of the oxalate ions was monitored by reflection absorption infrared spectroscopy (RAIRS). Helium ion microscopy (HIM) reveals the formation of spherical nanoparticles with well-defined size and X-ray photoelectron spectroscopy (XPS) confirms their metallic nature. Continued irradiation after depletion of oxalate does not lead to further particle growth giving evidence that nanoparticle formation is primarily controlled by the available amount of precursor. PMID:27547602

  17. Multiple Sulfate Isotopic Evidence on the Formation of Oxide Copper Ore at Spence, Atacama Desert, Northern Chile

    NASA Astrophysics Data System (ADS)

    Sun, T.; Bao, H.; Reich, M.; Palacios, C.

    2007-12-01

    In the Atacama Desert of northern Chile, one of the world's richest metallogenic provinces, porphyry copper deposits are characterized by the unique occurrence of atacamite in their oxidized zones. The origin and formation of the oxide zone of these copper deposits is, however, controversial. It was proposed that Cl-rich deep formation water pumping-up events along faults by earthquakes, after onset of the hyperaridity, were required (Cameron et al., 2007). Their model would imply that supplies of saline deep formation water from fractures to the surface should have left behind a homogeneous or fracture-controlled salt profile from surface down to the oxide zone. While no excluding the deep formation water model in other deposit, here we propose that, in our sampling region, the alternative saline source, which is critical for atacamite formation, could be locally evaporated groundwater, Cl-rich salts leached from arid surface by meteoric water, or brines from eastern salar basins at a time when the climate in northern Chile was changing from arid to hyperarid. At this climate transition, arid- requiring minerals such as atacamite in the oxide zone were formed and, more importantly, preserved upon evaporation beneath the surface alluvial deposits. Since salt accumulation at the surface remain active during hyperarid condition, our model would predict that water-soluble salt profile from surface to the oxide zone should have a characteristic pattern: salts with an atmospheric component on the surface gradually transitioning to salts of the oxide ore zone on the bottom and a mixing zone in between. To test these two alternative models, we focus on sulfate salts, one of the common water-soluble salts in arid environments. An added advantage is that sulfate accumulated on desert surface has a secondary atmospheric component that bears a unique triple oxygen isotope signature, easily distinguishable from sulfate formed by the oxidation of sulfide minerals at the oxide

  18. Persistent endosulfan sulfate is found with highest abundance among endosulfan I, II, and sulfate in German forest soils.

    PubMed

    Bussian, Bernd M; Pandelova, Marchela; Lehnik-Habrink, Petra; Aichner, Bernhard; Henkelmann, Bernhard; Schramm, Karl-Werner

    2015-11-01

    Endosulfan - an agricultural insecticide and banned by Stockholm Convention - is produced as a 2:1 to 7:3 mixture of isomers endosulfan I (ESI) and endosulfan II (ESII). Endosulfan is transformed under aerobic conditions into endosulfan sulfate (ESS). The study shows for 76 sampling locations in German forests that endosulfan is abundant in all samples with an opposite ratio between the ESI and ESII than the technical product, where the main metabolite ESS is found with even higher abundance. The ratio between ESI/ESII and ESS show clear dependence on the type of stands (coniferous vs. deciduous) and humus type and increases from deciduous via mixed to coniferous forest stands. The study argues for a systematic monitoring of ESI, ESII, and ESS and underlines the need for further research, specifically on the fate of endosulfan including biomagnifications and bioaccumulation in soil. PMID:26319511

  19. Proteomic analysis of acute responses to copper sulfate stress in larvae of the brine shrimp, Artemia sinica

    NASA Astrophysics Data System (ADS)

    Zhou, Qian; Wu, Changgong; Dong, Bo; Li, Fuhua; Liu, Fengqi; Xiang, Jianhai

    2010-03-01

    Proteomics was used to reveal the differential protein expression profiles of acute responses to copper sulfate exposure in larvae of Artemia sinica. Fourteen differentially displayed protein spots were detected and seven of them were identified. Three spots were up-expressed and identified: actin, heat shock protein 70, and chaperone subunit 1; three down-regulated proteins were identified: arginine kinase, elongation factor-2, and glycine-rich protein; and a newly expressed protein was identified as peroxiredoxin. The study indicates the involvement of all the differentially expressed proteins in the early responses of protein expression, and in the survival of A. sinica in the presence of copper and other heavy metals; the findings improve understanding of the organism’s adaptive responses and resistance.

  20. Adiabatic polymerization of acrylamide in water under the effect of the potassium persulfate-sodium metabisulfite-copper sulfate system

    SciTech Connect

    Kurenkov, V.F.; Baiburdov, T.A.; Stupen'kova, L.L.

    1988-04-10

    Since adiabatic polymerization of acrylamide (AA) has been studied very little and the information on the effect of copper ions on polymerization of AA prepared in dilute aqueous solutions is very limited, the features of adiabatic polymerization of AA in concentrated aqueous solutions in the presence of the potassium persulfate-sodium metabisulfite-copper sulfate redox initiating system were investigated in this study. The empirical equation for the overall rate of adiabatic polymerization of acrylamide in concentrated aqueous solutions was found, and the effective total activation energy, which decreases with an increase in the concentration of CuSO/sub 4/, was determined. An increase in the molecular weight of the polymer with an increase in the concentration of the monomer and a decrease in the concentration of the components of the initiating system was demonstrated.

  1. Copper(II) complexes with heterocyclic hydroxyimino-containing ligands

    SciTech Connect

    Kogan, V.A.; Burlov, A.S.; Popov, L.D.; Lukov, V.V.; Koshchienko, Yu.V.; Tsupak, E.B.; Barchan, G.P.; Chigarenko, G.G.; Bolotnikov, V.S.

    1988-05-01

    The reaction of oximes (R = Ph (L'), C=N (L'')) with the copper(II) salts CuA/sub 2/ in methanol has given the complexes CuL/sub 2/ ' x H/sub 2/O and CuL/sub 2//sup ''/ x 2H/sub 2/O (I) (A = Acet/sup -/), CuHLCl/sub 2/ x H/sub 2/O (II) (A = Cl/sup -/), CuLOH(ClO/sub 4/)/sub 2/ x 2H/sub 2/O (III) (A = ClO/sub 4//sup -/) and the complexes Cu/sub 3/L/sub 3//sup '/OH(NO/sub 3/)/sub 2/ and Cu/sub 3/L/sub 3//sup ''/(OH)/sub 2/NO/sub 3/ (IV) (A = NO/sub 3//sup -/). Their physicochemical properties have been studied by the methods of IR spectroscopy and magnetochemistry. It has been shown that complexes I have a chelate structure and that their magnetic moments are not dependent on the temperature. An anti-ferromagnetic exchange interaction takes place in complexes II-IV. On the basis of magnetochemical measurements over a broad temperature range and data calculated in the framework of the Heisenberg-Dirac-Van Vleck model of isotropic exchange interactions, a dimeric structure has been proposed for the complexes of type II, and a trinuclear cluster structure has been proposed for complexes III and IV.

  2. Sequestration of chelated copper by structural Fe(II): Reductive decomplexation and transformation of Cu(II)-EDTA.

    PubMed

    He, Hongping; Wu, Deli; Zhao, Linghui; Luo, Cong; Dai, Chaomeng; Zhang, Yalei

    2016-05-15

    Chelated coppers, such as Cu(II)-EDTA, are characteristically refractory and difficult to break down because of their high stability and solubility. Cu(II)-EDTA sequestration by structural Fe(II) (Fe(II)) was investigated intensively in this study. Up to 101.21mgCu(II)/gFe(II) was obtained by Fe(II) in chelated copper sequestration under near neutral pH condition (pH 7.70). The mechanism of Cu(II)-EDTA sequestration by Fe(II) was concluded as follows: 3Cu(II)-EDTA+7Fe(II)+9H2O → Cu(0)↓+ Cu2O↓(the major product)+2Fe2O3·H2O↓+3Fe(II)-EDTA +14H(+) Novel results strongly indicate that Cu(II) reductive transformation induced by surface Fe(II) was mainly responsible for chelated copper sequestration. Cu(0) generation was initially facilitated, and subsequent reduction of Cu(II) into Cu(I) was closely combined with the gradual increase of ORP (Oxidation-Reduction Potential). Cu-containing products were inherently stable, but Cu2O would be reoxidized to Cu(II) with extra-aeration, resulting in the release of copper, which was beneficial to Cu reclamation. Concentration diminution of Cu(II)-EDTA within the electric double layer and competitive adsorption were responsible for the negative effects of Ca(2+), Mg(2+). By generating vivianite, PO4(3-) was found to decrease surface Fe(II) content. This study is among the first ones to identify the indispensible role of reductive decomplexation in chelated copper sequestration. Given the high feasibility and reactivity, Fe(II) may provide a potential alternative in chelated metals pollution controlling. PMID:26878707

  3. The antimicrobial and antibiofilm activities of copper(II) complexes.

    PubMed

    Beeton, Michael L; Aldrich-Wright, Janice R; Bolhuis, Albert

    2014-11-01

    Biofilm-related bacterial infections pose a significant problem, as they are generally more tolerant to antibiotics and the immune system. Development of novel compounds with antibiofilm activity is therefore paramount. In this study we have analysed metal complexes of the general structure [M(IL)(AL)](2+) (where IL represents functionalised 1,10-phenanthrolines and AL represents 1S,2S- or 1R,2R-diaminocyclohexane) and [Cu(IL)3](2+). Antimicrobial activity was tested on a number of bacterial strains, showing that copper(II) compounds were active against both Gram-positive and Gram-negative bacteria, albeit that activity was generally higher for the former. The antibiofilm activity was then determined against a clinical isolate of meticillin-resistant Staphylococcus aureus (MRSA). Strikingly, the copper complexes tested showed significant activity against biofilms, and were better in the removal of biofilms than vancomycin, an antibiotic that is currently used in the treatment of MRSA infections. PMID:25124857

  4. Antifungal cobalt(II), copper(II), nickel(II) and zinc(II) complexes of furanyl-,thiophenyl-, pyrrolyl-, salicylyl- and pyridyl-derived cephalexins.

    PubMed

    Chohan, Zahid H; Pervez, Humayun; Khan, Khalid M; Rauf, A; Maharvi, Ghulam M; Supuran, Claudiu T

    2004-02-01

    Some novel cephalexin-derived furanyl, thiophenyl, pyrrolyl, salicylyl and pyridyl Schiff's bases and their cobalt (II), copper (II), nickel (II) and zinc (II) complexes have been synthesized and studied for their antifungal properties against Trichophyton longifusus, Candida albicans, Aspergillus flavus, Microsporum canis, Fusarium solani and Candida glaberata. The presence of metal ions in the investigated Schiff's base complexes reported here lead to significant antifungal activity, whereas the parent ligands were generally less active. PMID:15202498

  5. Differences in Median Ultraviolet Light Transmissions of Serial Homeopathic Dilutions of Copper Sulfate, Hypericum perforatum, and Sulfur

    PubMed Central

    Klein, Sabine D.; Sandig, Annegret; Baumgartner, Stephan; Wolf, Ursula

    2013-01-01

    Homeopathic remedies are produced by potentising, that is, the serial logarithmic dilution and succussion of a mother tincture. Techniques like ultraviolet spectroscopy, nuclear magnetic resonance, calorimetry, or thermoluminescence have been used to investigate their physical properties. In this study, homeopathic centesimal (c) potencies (6c to 30c) of copper sulfate, Hypericum perforatum, and sulfur as well as succussed water controls were prepared. Samples of these preparations were exposed to external physical factors like heat, pressure, ultraviolet radiation, or electromagnetic fields to mimic possible everyday storage conditions. The median transmissions from 190 nm to 340 nm and 220 nm to 340 nm were determined by ultraviolet light spectroscopy on five measurement days distributed over several months. Transmissions of controls and potencies of sulfur differed significantly on two of five measurement days and after exposure to physical factors. Transmissions of potencies exposed to ultraviolet light and unexposed potencies of copper sulfate and Hypericum perforatum differed significantly. Potency levels 6c to 30c were also compared, and wavelike patterns of higher and lower transmissions were found. The Kruskal-Wallis test yielded significant differences for the potency levels of all three substances. Aiming at understanding the physical properties of homeopathic preparations, this study confirmed and expanded the findings of previous studies. PMID:23401712

  6. Electrochemistry of Ferrous Sulfate-Sodium Thiosulphate and Copper Sulfate-Sodium Thiosulphate Systems for Template-Assisted Nanowire Synthesis

    NASA Astrophysics Data System (ADS)

    Brogan, Lee Jeffery

    Two related series of investigations are presented in this dissertation. First, two candidate systems for electrochemical deposition of metal sulfides for photovoltaic applications have been characterized. Secondly, a general electrochemical synthesis method allowing countable numbers of wires embedded in porous anodic alumina arrays to be measured using macroscopic contacts was developed. Electrochemical studies of the FeSO4-Na2S 2O3 system and the CuSO4-Na2S 2O3 system were undertaken to evaluate their suitability as electrodeposition baths for FeS2 and CuxS, respectively. Each solution system was studied extensively using cyclic voltammetry to characterize electrochemical processes at various concentrations. The iron sulfide / thiosulfate system was found to be unsuitable for the synthesis of FeS2 due to the preferential formation of FeS. The copper sulfide / thiosulfate system was found to be suitable for the synthesis of Cu2S, with thiosulfate concentration being the most important parameter due to the high complexation of Cu(I) by thiosulfate. Investigations into the electrochemical synthesis of metal wires in porous anodic alumina templates revealed an interesting synthesis mechanism wherein sparse, isolated wires are created in a very small fraction of the available pores. These wires are nucleated through the reduction of metal from the deposition bath by aluminum at the base of the alumina pores. This reduction causes a localized increase in acidity, accelerating the dissolution of the alumina barrier layer and allowing more typical wire deposition to occur. The sparse nucleation is exaggerated by the increasing rate of wire deposition as the wires lengthen and the swift rate of overgrowth formation at the surface of the template, resulting in domes of overgrowth attached to countable numbers of nanowires. This geometry has been exploited to obtain in situ measurements of known numbers of nanowires.

  7. Synthesis, superoxide dismutase, nuclease, and anticancer activities of copper(II) complexes incorporating bis(2-picolyl)amine with different counter anions

    NASA Astrophysics Data System (ADS)

    Ibrahim, Mohamed M.; Ramadan, Abdel-Motaleb M.; Mersal, Gaber A. M.; El-Shazly, Samir A.

    2011-07-01

    Interaction of the tridentate ligand bis(2-picolyl)amine L with copper(II) salts gave a series of copper(II) complexes with the formula types: [ LCu(X) 2] (X = Cl -1, = Br -2), [( LCu (H 2O)(μ-SO 4)( LCu(H 2O)]SO 43, [ LCu(OAc)](OAc )H 2O 4, [ LCu(H 2O) 2](Y) 2 (Y = NO3-5, = ClO4-6). Their structures and properties were characterized by elemental analysis, thermal analysis (TGA), IR, UV-vis and ESR spectroscopy, electrochemical measurements including cyclic voltammetry and electrical molar conductivity, and magnetic moment measurements. A square pyramidal geometry is proposed for the halogeno complexes 1 and 2 in monomeric structures. For sulfate complex, the sulfate group bridged two copper(II) ions of the two [N 3O] donor units to give the dimeric complex molecule 3 in square pyramidal environment around the copper(II) ions. In the case of complexes 4- 6, square planar stereochemistries in monomeric structures are suggested. The SOD biomimetic catalytic activity of the obtained complexes was assessed for their ability to inhibit the reduction of nitroblue tetrazolium (NBT). The catalytic efficiency of O2- scavenging by complexes depends on the nature of the particular acidic anion radical incorporated in the complex molecule and follows the order: NO3- > ClO4- > Br - ⩾ Cl - > SO4- > AcO -. A probable mechanistic implications for the catalytic dismutation of O2- by copper(II) complexes are proposed. Furthermore, complex 1 exhibits significant hydrolytic cleavage of the genomic DNA in the absence of any external additives. In addition, the in vitro study of cytotoxicity of complex 1 on colon cancer cell line (Caco-2) indicates that the complex has the potential to act as an effective anticancer drug with IC 50 value of 156 ± 0.35 μM.

  8. Development of Novel DNA Cleavage Systems Based on Copper Complexes. Synthesis and Characterisation of Cu(II) Complexes of Hydroxyflavones

    PubMed Central

    el Amrani, F. Ben-Allal; Perelló, L.; Torres, L.

    2000-01-01

    Copper(II) complexes of several hydroxyflavones were prepared and characterised through their physico-chemical properties. The nuclease activity of three synthesised complexes is reported. These copper(II) complexes present more nuclease activity than the ligands and the copper(II) ion. PMID:18475969

  9. Chelation of poly(methacryloylpiperidine) with copper (II) ions in aqueous solution

    SciTech Connect

    Zhaimina, G.M.; Babaev, T.M.; Bekturov, E.A.; Kudaibergenov, S.E.; Musaev, U.N.

    1985-04-20

    These authors use potentiometric and conductometric titration, spectroscopy, and viscosimetry to study interaction of copper ions with poly(methacryloylpiperidine) (PMAP) in aqueous solution. Charts present curves for potentiometric titration of PMAP hydrochloride in absence and in presence of copper(II) ions and for formation of PMAP/Cu/sup 2 +/, and for the dependence of optical density and conductivity on the Cu/sup 2 +//PMAP ratio. The authors conclude that copper(II) ions coordinate with two nitrogen atoms of piperidine units of the polyligand. The close proximity of donor groups in the principal macromolecular chain creates steric hindrance to the formation of the electronic configuration required for the copper(II) ions. They succeed in formation of intramolecular crosslinked structures in which copper(II) ions act as bridges.

  10. Effect of acute copper sulfate exposure on olfactory responses to amino acids and pheromones in goldfish (Carassius auratus).

    PubMed

    Kolmakov, Nikolay N; Hubbard, Peter C; Lopes, Orlando; Canario, Adelino V M

    2009-11-01

    Exposure of olfactory epithelium to environmentally relevant concentrations of copper disrupts olfaction in fish. To examine the dynamics of recovery at both functional and morphological levels after acute copper exposure, unilateral exposure of goldfish olfactory epithelia to 100 microM CuSO(4) (10 min) was followed by electro-olfactogram (EOG) recording and scanning electron microscopy. Sensitivity to amino acids (l-arginine and l-serine), generally considered food-related odorants, recovered most rapidly (three days), followed by that to catecholamines (3-O-methoxytyramine), bile acids (taurolithocholic acid) and the steroid pheromone, 17,20beta-dihydroxy-4-pregnen-3-one 20-sulfate, which took 28 days to reach full recovery. Sensitivity to the postovulatory pheromone prostaglandin F(2alpha) had not fully recovered even at 28 days. These changes in sensitivity were correlated with changes in the recovery of ciliated and microvillous receptor cell types. Microvillous cells appeared largely unaffected by CuSO(4) treatment. Cilia in ciliated receptor neurones, however, appeared damaged one day post-treatment and were virtually absent after three days but had begun to recover after 14 days. Together, these results support the hypothesis that microvillous receptor neurones detect amino acids whereas ciliated receptor neurones were not functional and are responsible for detection of social stimuli (bile acids and pheromones). Furthermore, differences in sensitivity to copper may be due to different transduction pathways in the different cell types. PMID:19924975

  11. Copper(II) interactions with nonsteroidal antiinflammatory agents. I. Salicylic acid and acetylsalicylic acid.

    PubMed

    Brumas, V; Brumas, B; Berthon, G

    1995-02-15

    Recently a growing body of evidence has accumulated on the beneficial effects of copper compounds toward various models of inflammation, and copper complexes of nonsteroidal antiinflammatory drugs (NSAIDs) have been shown to be more effective in this respect than the parent agents. However, the origin of this activity remains unclear: The ability of NSAIDs to influence copper metabolism is still questionable, and apart from the claimed SOD-like activity of copper salts in vivo, relatively little is known about how copper-NSAID interactions may help regulate the inflammatory process. Before the potential role of copper-NSAID complexes versus inflammation can be elucidated, speciation studies are necessary (i) to analyze the overall influence of these drugs on copper metabolism and (ii) to discriminate the individual complexes likely to represent the active form of the drug in vivo. In this paper, copper(II) complex equilibria with salicylic and acetylsalicylic acids--and benzoic acid used as a reference--as well as the mixed-ligand complex equilibria generated by these binary systems and L-histidine [main low-molar-mass ligand of copper(II) in blood plasma] have been investigated under physiological conditions (37 degrees C; 0.15-M NaCl). Confirming previous observations by others, resulting simulated plasma copper distributions virtually rule out any quantitative influence of salicylate on copper tissue diffusion at therapeutic levels. Even though, as is presently shown, both salicylate and acetylsalicylate may favor the gastrointestinal absorption of copper, it seems unlikely that salicylate can exert its antinflammatory activity predominantly through copper complexation. The assertion that copper-NSAID complexes represent the active forms of NSAIDs therefore seems to be of limited significance for salicylate. PMID:7876837

  12. Solvent effects on the stability of nifuroxazide complexes with cobalt(II), nickel(II) and copper(II) in alcohols.

    PubMed

    Khan, Mustayeen A; Ali, S Kauser; Bouet, Gilles M

    2002-05-21

    A spectrophotometric study of the complexation of nifuroxazide with cobalt(II), nickel(II) and copper(II) was carried out in different alcohols. The formation of a complex in each case is reported and their stability constants have been calculated. For a given solvent, the stability of the complexes increases from cobalt to copper. In the case of copper(II), the stability varies as an inverse function of the dielectric constant of the solvent. A possible structure of the complex is proposed. PMID:12009257

  13. Highly sensitive and selective fluorescence detection of copper (II) ion based on multi-ligand metal chelation.

    PubMed

    Zhang, Shan; Yu, Tao; Sun, Mingtai; Yu, Huan; Zhang, Zhongping; Wang, Suhua; Jiang, Hui

    2014-08-01

    A fluorescent probe was synthesized and demonstrated to be highly selective and sensitive in the reaction with copper (II) ion, generating a large variation of the fluorescence intensity in a dose-response manner. The probe contains a dansyl moiety as fluorophore and a multidentate ligand for copper (II) ion recognition. The reaction of the molecular probe with copper (II) ion proceeds rapidly and irreversibly in a 1 to 1 stoichiometric way, leading to the production of stable copper (II) complex, which subsequently results in the quenching of fluorescence. The detection limit for copper (II) ion was measured to be about 2ppb. It was also shown that the probe has high selectivity for copper (II) ion and good anti-interference ability against other transition metal ions. The herein reported very simple and reliable fluorescence probe could be employed for copper (II) ion detection in many aspects. PMID:24881551

  14. Effectiveness of copper sulfate, potassium permanganate, and peracetic acid to reduce mortality and infestation of Ichthyobodo nector in channel catfish Ictalurus punctatus (Rafinesque 1818)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ichthyobodo necator is a single celled bi-flagellate parasite, and in high density can causes significant mortality in young fish. Copper sulfate (CuSO4), potassium permanganate (KMnO4) and peracetic acid (PAA) were evaluated for effectiveness against ichthyobodosis. Treatments were: untreated con...

  15. Comparison of percent hatch and fungal infestation in channel catfish eggs after copper sulfate, diquat bromide, formalin, and hydrogen peroxide treatment

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Reduced survival is often a result of fungal (Saprolegnia spp.) infestation of fish eggs. However, timely chemical treatments often limit these infestations and increase survival. The effect of copper sulfate pentahydrate (CSP - 10 mg/L), diquat bromide (25 mg/L diquat cation), formalin (433 mg/L)...

  16. Comparing the efficacy of four immersion flush and static hydorgen peroxide and copper sulfate treatments on channel catfish eggs infected with water molds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Water mold infestations on channel catfish eggs lower the hatch rate (egg survival) and ultimately the number of catfish fry available for stocking in production ponds. This study compared the potential of two hydrogen peroxide (HP) and two copper sulfate pentahydrate (CSP) treatments to increase c...

  17. Efficacy of bath treatments of formalin and copper sulfate on cultured white bass, Morone chrysops, concurrently infected by Onchocleidus mimus and Ichthyophthirius multifiliis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Treatment efficacy of repeated 1 h baths of formalin (100 mg/L) or copper sulfate (CuSO4) (2.1 mg/L) were evaluated against concurrent infections of Onchocleidus mimus (Mueller, 1936; Monogenoidea: Ancyrocephalidae) and Ichthyophthirius multifiliis (Fouquet, 1876; Hymenostomatida: Ichthyopththiriida...

  18. The effectiveness of repeated tank treatments of copper sulfate and formalin on gill flukes and Ich in naturally infested white bass

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ectoparasites infesting the gills of juvenile fish reared in tanks can cause serious fish losses. Multiple treatments of formalin and copper sulfate (CuSO4) were evaluated for effectiveness against a natural infestation of gill flukes Onchocleidus mimus (Monogenoidea: Ancyrocephalidae) and Ichthyop...

  19. Assessment of Aquaflor (c), copper sulfate and potassium permanganate for control of Aeromonas hydrophila and Flavobacterium columnare infection in sunshine bass, Morone chrysops female x Morone saxatilis male

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two experiments were conducted to assess different therapeutants against a mixed infection of Aeromonas hydrophila and Flavobacterium columnare in sunshine bass (SB) (Morone chrysops female x Morone saxatilis male). Experiment 1 assessed the efficacy of copper sulfate (CuSO4), florfenicol-medicated...

  20. The effectiveness of flow-through and/or static copper sulfate treatments on the survival of Golden Shiners and Fathead Minnows infected with Flavobacterium columare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Four studies were done comparing various treatments of copper sulfate (CuSO4) on the survival of golden shiner and fathead minnow (both economically important baitfish) that had columnaris disease. The treatments were observed for their ability to reduce mortality in well water. In experiment 1 and ...

  1. The effectiveness of flow-through and/or static copper sulfate treatments on the survival of Golden Shiners and Fathead Minnows infected with Flavobacterium columare

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Four studies were done comparing various treatments of copper sulfate (CuSO4) on the survival of golden shiner and fathead minnow (both economically important baitfish) that had columnaris disease. The treatments were observed for their ability to reduce mortality in well water. In experiment 1 an...

  2. Growth and characterization of Cu (II) doped negatively soluble lithium sulfate monohydrate crystals

    NASA Astrophysics Data System (ADS)

    Boopathi, K.; Ramasamy, P.; Bhagavannarayana, G.

    2014-01-01

    Single crystals of pure and Cu (II) doped negatively soluble lithium sulfate monohydrate have been grown by slow evaporation solution technique. In the present work, to improve the crystalline quality of lithium sulfate monohydrate crystal, metal dopant was incorporated into the pure crystals. The as grown crystals are clear, transparent and the sizes of the crystals were up to 18×12×3 mm3 and 50×15×5 mm3. The presence of metal dopant has been confirmed by energy dispersive spectroscopy, atomic absorption spectroscopy analysis. Single crystal and powder X-ray diffraction studies were carried out to ascertain lattice parameters and identify different phase nature. Optical transmission spectrum of the grown crystals was recorded. FT-IR and thermal analysis were carried out to investigate the functional group and thermal behavior of the grown crystals respectively. The grown crystal was subjected to Vickers micro hardness, HRXRD, piezoelectric, laser damage threshold measurements and second harmonic generation efficiency studies.

  3. Spectral studies of copper(II) complexes of 6-(3-thienyl) pyridine-2-thiosemicarbazone

    NASA Astrophysics Data System (ADS)

    Mahjoub, Omima Abdalla; Farina, Yang

    2014-09-01

    Two novel copper(II) complexes [Cu(HL)Cl]Cl˙H2O (1) and [Cu(L)NO3]˙H2O (2) of the three NNS donor thiosemicarbazone ligand 6-(3-thienyl) pyridine-2-thiosemicarbazone have been synthesized. The ligand and its copper(II) complexes were characterized by elemental analysis (C, H, N, and S), FT-IR, UV-visible, magnetic susceptibility and molar conductance. The thiosemicarbazone is present either as the thione form in complex 1 or as thiol form in complex 2 and is coordinated to copper(II) atom via the pyridine nitrogen atom, the azomethine nitrogen atom and the sulfur atom. The physicochemical and spectral data suggest square planar geometry for copper(II) atoms.

  4. A microcalorimetric study of the sorption of copper(II) on KB-4 carboxyl cationite

    NASA Astrophysics Data System (ADS)

    Zauer, E. A.

    2008-08-01

    The thermokinetics of sorption of copper(II) ions on KB-4 carboxyl cationite in the salt and hydrogen forms was studied microcalorimetrically. The total heat effect of sorption and its dependence on the degree of ionite filling were determined.

  5. Spectral studies of copper(II) complexes of 6-(3-thienyl) pyridine-2-thiosemicarbazone

    SciTech Connect

    Mahjoub, Omima Abdalla; Farina, Yang

    2014-09-03

    Two novel copper(II) complexes [Cu(HL)Cl]Cl.H{sub 2}O (1) and [Cu(L)NO{sub 3}]Ðœ‡H{sub 2}O (2) of the three NNS donor thiosemicarbazone ligand 6-(3-thienyl) pyridine-2-thiosemicarbazone have been synthesized. The ligand and its copper(II) complexes were characterized by elemental analysis (C, H, N, and S), FT-IR, UV-visible, magnetic susceptibility and molar conductance. The thiosemicarbazone is present either as the thione form in complex 1 or as thiol form in complex 2 and is coordinated to copper(II) atom via the pyridine nitrogen atom, the azomethine nitrogen atom and the sulfur atom. The physicochemical and spectral data suggest square planar geometry for copper(II) atoms.

  6. Histopathological and bacterial study of skin and gill of grass carp, Ceteopharyngodon idella, (Valenciennes 1844) exposed to copper sulfate and potassium permanganate.

    PubMed

    Jooyandeh, Fatemeh; Sadeghpour, Ali; Khara, Hossein; Pajand, Zabihollah

    2016-09-01

    The gill histology and bacterial load of skin of the grass carp juveniles were investigated in relation to various concentrations of copper sulfate and potassium permanganate. For this purpose, the sublethal doses were determined after a pre-test and then the experiment was done in five treatments (for copper sulfate: 1, 1.94, 3.71, 7.07 and 15 mg/l and for potassium permanganate: 0.25, 0.52, 1.91, 2.27 and 5 mg/l) with three replicates inside the glass aquaria. Also, one group without disinfecting product was considered as control for each experiment. The microbial and histopathological investigations were done after 96 h exposure. According to results, the lowest bacterial load (CFU/g) of skin was observed in 15 mg/l copper sulfate treatment and 0.25 mg/l potassium permanganate treatment (P < 0.05). Also, the histological investigation showed a range of histopathological alternations in gills tissue including lamellar necrosis, hyperplasia, lamellar adhesion, haemorrhage, clubbing of gill lamellae. The severity of these alternations increased with increasing of the doses of the copper sulfate and potassium permanganate. In this regard, the highest histological damages were observed in 15 mg/l copper sulfate and 5 mg/l potassium permanganate respectively. Our results showed that low dosage of potassium permanganate has best effect on reducing of bacterial load of skin with lowest adverse effects on gill tissue. PMID:27605829

  7. 78 FR 78727 - Copper Sulfate Pentahydrate; Exemption From the Requirement of a Tolerance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-27

    ..., 2013 (78 FR 43115) (FRL-9392- 9), EPA issued a document pursuant to FFDCA section 408, 21 U.S.C. 346a... final rule published in the Federal Register of August 11, 2006 (71 FR 46106) (FRL-8085-3). Copper is... review under Executive Order 12866, entitled ``Regulatory Planning and Review'' (58 FR 51735, October...

  8. Spectroscopic characterization of copper(II) complexes of indoxyl N(4)-methyl thiosemicarbazone

    NASA Astrophysics Data System (ADS)

    Chandra, Sulekh; Kumar, Umendra

    2004-10-01

    New copper(II) complexes of indoxyl thiosemicarbazone (ITSC) of general composition CuL 2X 2 (where L: ITSC; X: Cl -, NO 3-, ClO 4-, NCS -) have been synthesized and characterized by elemental analysis, molar conductance, magnetic susceptibility measurements and spectral (electronic, IR, EPR, 1H NMR , Mass) studies. Cyclic voltammetry measurements show quasi-reversible Cu 2+/Cu 1+ couple. Various physico-chemical techniques suggest a tetragonal structure for these copper(II) complexes.

  9. [Treating Cr(VI)-containing wastewater by a consortium of sulfate reducing bacteria and copper-iron bimetallic particles].

    PubMed

    He, Qi-Zhi; Chen, Hui; Wang, Dan; Li, Hua; Ding, Xing-Hu; Deng, Le

    2011-07-01

    Copper-iron bimetallic particles were prepared by chemical precipitation technique. Under the help of the particles Cr(VI)-containing wastewater was well treated by a consortium of sulfate reducing bacteria, which were enriched from industrial wastewater and acclimatized to tolerant to high concentrations of Cr(VI). SRB-Cu/Fe system, traditional SRB system and Cu/Fe system were experimented in the batch bioreactor, respectively. It is demonstrated that SRB-Cu/Fe bimetallic system perform much better than traditional SRB system or copper-iron bimetallic. The acclimation period of SRB was significantly reduced and the inhibiting concentration of Cr(VI) of SRB was also greatly increased by approximately 200% (from 100 mg x L(-1) to 300 mg x L(-1)). Under the conditions of Cr(VI) 300 mg x L(-1), Q(Cu)/Fe 7.5%, pH 5.0-8.0, the concentration of total chromium was less than 0.512 mg/L, Cr(VI) less than 0.071 mg, Cu next to zero after 48 h treatment. Having biological, chemical advantages and high efficiency, the novel SRB-Cu/Fe system should have the broad application prospects in industrial wastewater. PMID:21922821

  10. The role of copper(II) in the aggregation of human amylin.

    PubMed

    Sinopoli, Alessandro; Magrì, Antonio; Milardi, Danilo; Pappalardo, Matteo; Pucci, Pietro; Flagiello, Angela; Titman, Jeremy J; Nicoletti, Vincenzo Giuseppe; Caruso, Giuseppe; Pappalardo, Giuseppe; Grasso, Giuseppe

    2014-10-01

    Amylin is a 37-residue peptide hormone produced by the islet β-cells of pancreas and the formation of amylin aggregates is strongly associated with β-cell degeneration in type 2 diabetes, as demonstrated by more than 95% of patients exhibiting amylin amyloid upon autopsy. It is widely recognized that metal ions such as copper(II) have been implicated in the aggregation process of amyloidogenic peptides such as Aβ and α-synuclein and there is evidence that amylin self-assembly is also largely affected by copper(II). For this reason, in this work, the role of copper(II) in the aggregation of amylin has been investigated by several different experimental approaches. Mass spectrometric investigations show that copper(II) induces significant changes in the amylin structure, which decrease the protein fibrillogenesis as observed by ThT measurements. Accordingly, solid-state NMR experiments together with computational analysis carried out on a model amylin fragment confirmed the non-fibrillogenic nature of the copper(II) induced aggregated structure. Finally, the presence of copper(II) is also shown to have a major influence on amylin proneness to be degraded by proteases and cytotoxicity studies on different cell cultures are reported. PMID:25080969

  11. Synthesis, spectroscopic and thermal studies of the copper(II) aspartame chloride complex

    NASA Astrophysics Data System (ADS)

    Çakır, S.; Coşkun, E.; Naumov, P.; Biçer, E.; Bulut, İ.; İçbudak, H.; Çakır, O.

    2002-08-01

    Aspartame adduct of copper(II) chloride Cu(Asp) 2Cl 2·2H 2O (Asp=aspartame) is synthesized and characterized by elemental analysis, FT IR, UV/vis, ESR spectroscopies, TG, DTG, DTA measurements and molecular mechanics calculations. Aqueous solution of the green solid absorbs strongly at 774 and 367 nm. According to the FT IR spectra, the aspartame moiety coordinates to the copper(II) ion via its carboxylate ends, whereas the ammonium terminal groups give rise to hydrogen bonding network with the water, the chloride ions or neighboring carboxylate groups. The results suggest tetragonally distorted octahedral environment of the copper ions.

  12. Diffusion behavior of copper atoms under Cu(II) reduction in Cucurbit[8]uril cavity at elevated temperatures

    SciTech Connect

    Bakovets, Vladimir V.; Nadolinnii, Vladimir A.; Kovalenko, Ekaterina A.; Plyusnin, Pavel E.; Dolgovesova, Irina P.; Zaikovskii, Vladimir I.

    2015-01-15

    In this paper we describe copper clusters and nanoparticles formation by the reduction of copper (II) ions inside cavities of macrocycle molecules using supramolecular compound [Cu(Cyclen)(H{sub 2}O)@CB[8

  13. Structural Diversity of Copper(II) Complexes with 9-Deazahypoxanthine and Their in Vitro SOD-Like Activity

    PubMed Central

    Gáliková, Jana; Trávníček, Zdeněk

    2015-01-01

    Two structurally different copper(II) complexes of the compositions [{Cu(9dhx)(H2O)3}2(µ-SO4)2] (1) and [Cu(9dhx)2(H2O)2(NO3)2]·H2O (2), involving 9-deazahypoxanthine (9dhx; 6-oxo-9-deazapurine; 9-deazahypoxanthine), have been prepared and characterized by elemental analysis, infrared and electronic spectroscopy, electrospray ionisation (ESI) mass spectrometry, thermogravimetric (TG) and differential thermal (DTA) analyses, and cyclic voltammetry. The X-ray structures of complexes 1 and [Cu(9dhx)2(H2O)2(NO3)2] (2a) revealed the distorted octahedral geometry in the vicinity of the copper(II) atoms, with the NO5 and N2O4 donor set, respectively. In the dimeric compound 1, the {Cu(9dhx)(H2O)3}2 units are bridged by sulfate groups with the Cu···Cu separation being 5.3446(2) Å. In both structures the 9dhx ligands are coordinated through the N3 atoms of the pyrimidine moieties. The SOD-like activity of complexes 1 and 2 was evaluated in vitro showing moderate effect, with the IC50 values equal to 18.20, and 53.33 μM, respectively. PMID:26184182

  14. Structural Diversity of Copper(II) Complexes with 9-Deazahypoxanthine and Their in Vitro SOD-Like Activity.

    PubMed

    Gáliková, Jana; Trávníček, Zdeněk

    2015-01-01

    Two structurally different copper(II) complexes of the compositions [{Cu(9dhx)(H2O)3}2(µ-SO4)2] (1) and [Cu(9dhx)2(H2O)2(NO3)2]·H2O (2), involving 9-deazahypoxanthine (9dhx; 6-oxo-9-deazapurine; 9-deazahypoxanthine), have been prepared and characterized by elemental analysis, infrared and electronic spectroscopy, electrospray ionisation (ESI) mass spectrometry, thermogravimetric (TG) and differential thermal (DTA) analyses, and cyclic voltammetry. The X-ray structures of complexes 1 and [Cu(9dhx)2(H2O)2(NO3)2] (2a) revealed the distorted octahedral geometry in the vicinity of the copper(II) atoms, with the NO5 and N2O4 donor set, respectively. In the dimeric compound 1, the {Cu(9dhx)(H2O)3}2 units are bridged by sulfate groups with the Cu···Cu separation being 5.3446(2) Å. In both structures the 9dhx ligands are coordinated through the N3 atoms of the pyrimidine moieties. The SOD-like activity of complexes 1 and 2 was evaluated in vitro showing moderate effect, with the IC50 values equal to 18.20, and 53.33 μM, respectively. PMID:26184182

  15. Iron versus Copper II. Principles and Applications in Bioinorganic Chemistry.

    ERIC Educational Resources Information Center

    Ochiai, Ei-Ichiro

    1986-01-01

    Discusses the differences between iron and copper. Describes various aspects of the behaviors of these two elements, including those of biological and environmental significance. Addresses the evolution of the atmosphere and sedimentary ore formation, the phylogeny of iron and copper, and some anthropological notes regarding the use of the metals.…

  16. Pyrrolidine dithiocarbamate-zinc(II) and -copper(II) complexes induce apoptosis in tumor cells by inhibiting the proteasomal activity

    SciTech Connect

    Milacic, Vesna; Chen Di; Giovagnini, Lorena; Diez, Alejandro; Fregona, Dolores; Dou, Q. Ping

    2008-08-15

    Zinc and copper are trace elements essential for proper folding, stabilization and catalytic activity of many metalloenzymes in living organisms. However, disturbed zinc and copper homeostasis is reported in many types of cancer. We have previously demonstrated that copper complexes induced proteasome inhibition and apoptosis in cultured human cancer cells. In the current study we hypothesized that zinc complexes could also inhibit the proteasomal chymotrypsin-like activity responsible for subsequent apoptosis induction. We first showed that zinc(II) chloride was able to inhibit the chymotrypsin-like activity of a purified 20S proteasome with an IC{sub 50} value of 13.8 {mu}M, which was less potent than copper(II) chloride (IC{sub 50} 5.3 {mu}M). We then compared the potencies of a pyrrolidine dithiocarbamate (PyDT)-zinc(II) complex and a PyDT-copper(II) complex to inhibit cellular proteasomal activity, suppress proliferation and induce apoptosis in various human breast and prostate cancer cell lines. Consistently, zinc complex was less potent than copper complex in inhibiting the proteasome and inducing apoptosis. Additionally, zinc and copper complexes appear to use somewhat different mechanisms to kill tumor cells. Zinc complexes were able to activate calpain-, but not caspase-3-dependent pathway, while copper complexes were able to induce activation of both proteases. Furthermore, the potencies of these PyDT-metal complexes depend on the nature of metals and also on the ratio of PyDT to the metal ion within the complex, which probably affects their stability and availability for interacting with and inhibiting the proteasome in tumor cells.

  17. Pyrrolidine dithiocarbamate-zinc(II) and -copper(II) complexes induce apoptosis in tumor cells by inhibiting the proteasomal activity☆

    PubMed Central

    Milacic, Vesna; Chen, Di; Giovagnini, Lorena; Diez, Alejandro; Fregona, Dolores; Dou, Q. Ping

    2013-01-01

    Zinc and copper are trace elements essential for proper folding, stabilization and catalytic activity of many metalloenzymes in living organisms. However, disturbed zinc and copper homeostasis is reported in many types of cancer. We have previously demonstrated that copper complexes induced proteasome inhibition and apoptosis in cultured human cancer cells. In the current study we hypothesized that zinc complexes could also inhibit the proteasomal chymotrypsin-like activity responsible for subsequent apoptosis induction. We first showed that zinc(II) chloride was able to inhibit the chymotrypsin-like activity of a purified 20S proteasome with an IC50 value of 13.8 μM, which was less potent than copper(II) chloride (IC50 5.3 μM). We then compared the potencies of a pyrrolidine dithiocarbamate (PyDT)-zinc(II) complex and a PyDT-copper(II) complex to inhibit cellular proteasomal activity, suppress proliferation and induce apoptosis in various human breast and prostate cancer cell lines. Consistently, zinc complex was less potent than copper complex in inhibiting the proteasome and inducing apoptosis. Additionally, zinc and copper complexes appear to use somewhat different mechanisms to kill tumor cells. Zinc complexes were able to activate calpain-, but not caspase-3-dependent pathway, while copper complexes were able to induce activation of both proteases. Furthermore, the potencies of these PyDT-metal complexes depend on the nature of metals and also on the ratio of PyDT to the metal ion within the complex, which probably affects their stability and availability for interacting with and inhibiting the proteasome in tumor cells. PMID:18501397

  18. Synthesis and crystal structure of thiosemicarbazide complexes of nickel(II) and copper(II)

    NASA Astrophysics Data System (ADS)

    Sadikov, G. G.; Antsyshkina, A. S.; Koksharova, T. V.; Sergienko, V. S.; Kurando, S. V.; Gritsenko, I. S.

    2012-07-01

    Thiosemicarbazide complexes of nickel(II) [Ni( TSC)2](H Sal)2 ( I) and copper(II) [Cu( TSC)2](H Sal)2 ( Ia) ( TSC is thiosemicarbazide and H Sal is a salycilate anion), as well as complexes [Ni( TSC)2](SO4) · 2H2O ( II) and [Ni( TSC)3]Cl2 · H2O ( III), are synthesized and characterized by IR spectroscopy and X-ray diffraction. Monoclinic crystals I and Ia are isostructural; space group P21/ n, Z = 2. Crystals II are monoclinic, space group P21/ m, Z = 2. Crystals III are orthorhombic, space group Pbca, Z = 8. In I and Ia, two planar salycilate anions sandwich a planar centrosymmetric [Ni( TSC)2]2+ cation to form a supermolecule. The cation and anions are additionally bound by hydrogen bonds. Other hydrogen bonds connect supermolecules into planar layers. In structure II, centrosymmetric [Ni( TSC)2]2+ cations are connected by π-stacking interactions into supramolecular ensembles of a specific type. The ensembles, water molecules, and (SO4)2- anions are bound in the crystal via hydrogen bonds. In the [Ni( TSC)3]2+ cation of structure III, ligands coordinate the Ni atom by the bidentate chelate pattern with the formation of five-membered metallocycles. These metallocycles have an envelope conformation unlike those in I and II, which are planar. In III (unlike in analogous complexes), a meridional isomer of the coordination octahedron of the Ni atom is formed. Together with Cl1- and Cl2- anions, cations form supermolecules, which are packed into planar layers with a square-cellular structure. The layers are linked by hydrogen bonds formed by crystallization water molecules that are located between the layers.

  19. Mercury bioaccumulation and bioaccumulation factors for Everglades mosquitofish as related to sulfate: a re-analysis of Julian II (2013).

    PubMed

    Pollman, Curtis D; Axelrad, Donald M

    2014-11-01

    The Everglades, an ecosystem of international significance, has elevated biota mercury levels representing risk to human and wildlife consumers of fish. Given the critical role of sulfate in the methylation of mercury, and because there is a significant agricultural contribution, one potential means of reducing these mercury levels is reducing Everglades sulfate inputs. Julian II (Bull Environ Contam Toxicol 90:329-332, 2013) conducted regression modeling of the relationship between surface water sulfate concentrations and Gambusia spp. mercury bioconcentration factors across the major hydrologic subunits of the Everglades, and used those results to draw conclusions about the role of sulfate in the cycling of mercury in the Everglades. We however demonstrate a number of fundamental problems with the analysis, interpretation and conclusions. As a result, we strongly caution against using the results of Julian II (Bull Environ Contam Toxicol 90:329-332, 2013) to formulate management decisions regarding mitigation of the Everglades mercury problem. PMID:25260994

  20. Iron(II) sulfate release from drop-formed lipophilic matrices developed by special hot-melt technology.

    PubMed

    Pallagi, E; Vass, K; Pintye-Hódi, K; Kása, P; Falkay, G; Eros, I; Szabó-Révész, P

    2004-03-01

    Iron(II) sulfate-containing lipophilic matrices were developed by a special hot-melt technology (melt solidification in drops), using stearin, white wax and their mixture as conventional bed materials. The special technology resulted in spherical particles which can be filled directly into capsules; these store iron as a depot and ensure a slow and uniform release, whereby the irritation of the gastric mucosa by the iron can be decreased. The rates of dissolution of the iron(II) sulfate from the various lipophilic matrices were different, but fundamentally low. Kinetic calculations demonstrated that the rate of dissolution of the iron(II) sulfate was of approximately zero kinetic order. The results of in vivo experiments on rabbits correlated well with the in vitro data. The plasma curves for the animals treated with the iron(II) sulfate preparations varied with the excipients in the depot products. The properties and ratio of the bed materials influenced the release of the iron(II) sulfate. In all probability, the release of the active agent can be regulated through the use of a melt of stearin and white wax in different ratios. The development products functioned as a sustained-release system and ensured elimination of the irritation of the gastric mucosa. At the same time, the results justified the applicability of the special hot-melt technology in the development of the solid dosage form. PMID:15018986

  1. Aminopropyl-modified mesoporous silica SBA-15 as recovery agents of Cu(II)-sulfate solutions: Adsorption efficiency, functional stability and reusability aspects.

    PubMed

    Lombardo, M V; Videla, M; Calvo, A; Requejo, F G; Soler-Illia, G J A A

    2012-07-15

    Hybrid mesoporous materials are potentially useful for metal ion scavenging and retrieval because of their high surface areas, controlled accessibility and tailored functionalization. Some aspects that are linked to the performance of HMM include pore accessibility, stability of the organic functions and reusability. Knowledge of these aspects is critical in the design of adsorption-desorption protocols. In this work we produce and characterize propylamino-substituted large pore silica (SBA-15-N), which is submitted to Cu(II) adsorption from copper sulfate solutions, followed by desorption in acid media and material regeneration. We find that the hybrid material is an efficient adsorbent (1.15-1.75mmol Cu(II)g(-1)), although a fraction of the organic groups is lost during the adsorption process. An X-ray photoelectron spectroscopy (XPS) study demonstrates that the contents of amino groups are higher in the material surface, leading to different behaviors in Cu(II) complexation along the material. These materials can be regenerated by exposure to acidic media. Thermal processing of the hybrid materials leads to better durability in aqueous solutions during reprocessing, due to enhanced polycondensation of the inorganic framework. Thermally treated samples, once regenerated, are efficient adsorbents in a second step of Cu(II) adsorption. We discuss the materials processing factors involved in the improved adsorption of Cu(II), its quantitative release and reusability of the material. PMID:22595542

  2. Highly enantioselective Henry reactions of aromatic aldehydes catalyzed by an amino alcohol-copper(II) complex.

    PubMed

    Qin, Dan-Dan; Lai, Wen-Han; Hu, Di; Chen, Zheng; Wu, An-An; Ruan, Yuan-Ping; Zhou, Zhao-Hui; Chen, Hong-Bin

    2012-08-20

    Amino alcohol-Cu(II) catalyst: Highly enantioselective Henry reactions between aromatic aldehydes and nitromethane have been developed. The reactions were catalyzed by an easily available and operationally simple amino alcohol-copper(II) catalyst. In total, 38 substrates were tested and the R-configured products were obtained in good yields with excellent enantioselectivities. PMID:22791567

  3. Structural characterization of some oxalato-bridged copper(II) and nickel(II) complexes

    NASA Astrophysics Data System (ADS)

    Mautner, Franz A.; Louka, Febee R.; Massoud, Salah S.

    2009-03-01

    A new series of dinuclear oxalato-bridged copper(II) and nickel(II) complexes derived from tridentate amines: [Cu 2(Medpt) 2(μ-C 2O 4)](ClO 4) 2 ( 1), [Cu 2(pmedien) 2(μ-C 2O 4)](ClO 4) 2 ( 2), [Cu 2(DPA) 2(μ-C 2O 4)(ClO 4) 2]·2H 2O (3), and [Ni 2(Et 2dien) 2(μ-C 2O 4)(H 2O) 2](ClO 4) 2·2H 2O ( 4) (C 2O 42- = oxalate dianion, Medpt = 3,3'-diamino- N-methyldipropylmine, pmedien = N, N, N', N'', N''-pentamethyldiethylenetriamine, DPA = di(2-pyridylmethyl)amine and Et 2dien = N, N-diethyldiethylenetriamine) were synthesized and structurally characterized by X-ray crystallography. The spectral and structural characterizations of these complexes are reported. In this series, structures consist of the C 2O 42- bridging the two M(II) centers in a bis-bidentate bonding mode. The Cu 2+ centers are coordinated to the tridentate amines Medpt or pmedien in distorted SP geometry in 1 and 2, respectively, with the ClO 4- groups as counter ions. In the other two complexes, distorted octahedral geometries were achieved by the three nitrogen donors of the DPA and by an oxygen atom from coordinated perchlorate ion in 3 and by the Et 2dien and one water molecule in 4.

  4. Effect of phosphate and sulfate on Ni repartitioning during Fe(II)-catalyzed Fe(III) oxide mineral recrystallization

    NASA Astrophysics Data System (ADS)

    Hinkle, Margaret A. G.; Catalano, Jeffrey G.

    2015-09-01

    Dissolved Fe(II) activates coupled oxidative growth and reductive dissolution of Fe(III) oxide minerals, causing recrystallization and the repartitioning of structurally-compatible trace metals. Phosphate and sulfate, two ligands common to natural aquatic systems, alter Fe(II) adsorption onto Fe(III) oxides and affect Fe(III) oxide dissolution and precipitation. However, the effect of these oxoanions on trace metal repartitioning during Fe(II)-catalyzed Fe(III) oxide recrystallization is unclear. The effects of phosphate and sulfate on Ni adsorption and Ni repartitioning during Fe(II)-catalyzed Fe(III) oxide recrystallization were investigated as such repartitioning may be affected by both Fe(II)-oxoanion and metal-oxoanion interactions. In most systems examined, phosphate alters Ni repartitioning during Fe(II)-catalyzed recrystallization to a larger extent than sulfate. Phosphate substantially enhances Ni adsorption onto hematite but decreases (nearly inhibiting) Fe(II)-catalyzed Ni incorporation into and release from this mineral. In the goethite system, however, phosphate suppresses Ni release but enhances Ni incorporation in the presence of aqueous Fe(II). In contrast, sulfate has little effect on macroscopic Ni adsorption and release of Ni from Fe(III) oxides, but substantially enhances Ni incorporation into goethite. This demonstrates that phosphate and sulfate have unique, mineral-specific interactions with Ni during Fe(II)-catalyzed Fe(III) oxide recrystallization. This research suggests that micronutrient bioavailability at redox interfaces in hematite-dominated systems may be especially suppressed by phosphate, while both oxoanions likely have limited effects in goethite-rich soils or sediments. Phosphate may also exert a large control on contaminant fate at redox interfaces, increasing Ni retention on iron oxide surfaces. These results further indicate that trace metal retention by iron oxides during lithification and later repartitioning during

  5. Precipitation and Characterization of Arsenate Phases from Calcium-Copper-Iron-Arsenic Oxide-Sulfate Hydrothermal Systems

    NASA Astrophysics Data System (ADS)

    Gomez, Mario Alberto

    The scope of this thesis is the study of three Fe(III)-As(V) hydrothermal systems. The first one is the Fe(III)-AsO4-SO 4 system and crystalline phases that are produced under high temperature (150-225°C); this was studied to clear up previous contradicting information on this system in relation to industrial arsenic products that are formed during the autoclave processing of arsenical sulphide gold feedstocks and asses their arsenic stability. The second system studied was Cu(II)-Fe(III)-AsO 4-SO4 system at 150°C; this was investigated due to its relevance to industrial pressure leaching of copper concentrates. This system was studied in order to examine the possible effect of copper on the precipitation of scorodite. Finally, the structural and molecular examination of two members of the Ca(II)-Fe(III)-AsO4 system, namely yukonite (synthetic and natural and arseniosiderite was undertaken due to their relatively unknown nature and the potential role play in controlling arsenic release in tailings.

  6. Near-infrared surface-enhanced Raman spectroscopy. Part II: Copper and gold colloids

    SciTech Connect

    Angel, S. M.; Katz, L. F.; Archibald, D. D.; Honigs, D. E.

    1989-03-01

    Near-infrared (NIR) surface-enhanced Raman spectra (SERS) on copper and gold metal colloids were obtained with a Fourier transform Raman spectrometer using a Nd:YAG laser (1.064 ..mu..m) for excitation. Enhanced spectra were observed for pyridine and 3-chloropyridine (CP) on copper colloids and for tris(orthophenanthroline)ruthenium(II), Ru(o-phen)/sup 2 +//sub 3/, on copper and gold colloids. The copper-colloid surface-enhanced Raman spectra of pyridine and CP were compared with spectra measured for these molecules on copper electrodes. NIR-SERS enhancements on the metal colloids were at least as large as for visible-wavelength excited SERS. Good-quality spectra of Ru(o-phen)/sup 2 +//sub 3/ were obtained at solution concentrations as low as 0.025 mM.

  7. DNA/RNA binding and anticancer/antimicrobial activities of polymer-copper(II) complexes

    NASA Astrophysics Data System (ADS)

    Lakshmipraba, Jagadeesan; Arunachalam, Sankaralingam; Riyasdeen, Anvarbatcha; Dhivya, Rajakumar; Vignesh, Sivanandham; Akbarsha, Mohammad Abdulkader; James, Rathinam Arthur

    2013-05-01

    Water soluble polymer-copper(II) complexes with various degrees of coordination in the polymer chain were synthesized and characterized by elemental analysis, IR, UV-visible and EPR spectra. The DNA/RNA binding behavior of these polymer-copper(II) complexes was examined by UV-visible absorption, emission and circular dichroism spectroscopic methods, and cyclic voltammetry techniques. The binding of the polymer-copper(II) complexes with DNA/RNA was mainly through intercalation but some amount of electrostatic interaction was also observed. This binding capacity increased with the degree of coordination of the complexes. The polymer-copper(II) complex having the highest degree of coordination was subjected to analysis of cytotoxic and antimicrobial properties. The cytotoxicity study indicated that the polymer-copper(II) complexes affected the viability of MCF-7 mammary carcinoma cells, and the cells responded to the treatment with mostly through apoptosis although a few cells succumbed to necrosis. The antimicrobial screening showed activity against some human pathogens.

  8. Sodium lauryl sulfate-ruthenium(II) interactions: Photogalvanic and photophysical behavior of Ru(II)-diimine complexes

    SciTech Connect

    Akasheh, T.S.; Al-Rawashdeh, N.A.F. )

    1990-11-15

    The photogalvanic and photophysical behavior of a number of mixed-ligand ruthenium(II) complexes of 2,2{prime}-bipyridine (bpy), 3,3{prime}-bipyradzine (bpd), 2-(2{prime}-pyridyl)quinoline (pyrq), 4,4{prime}-dimethyl-2,2{prime}-bipyridine (dmbpy), and 2,3-bis(2{prime}-pyridyl)pyrazine (dpp) is reported both in water and in sodium lauryl sulfate (SDS) solutions. The effect of SDS on the photogalvanic experiment is predominantly an enhancement and/or modification of the photochemical and electrochemical processes. Luminescence, lifetime measurements, and photochemical behavior are affected by SDS and are used to predict possible modes of micelle-complex interactions.

  9. Dialkyl pyridinedicarboxylates` extraction ability toward copper(II) from chloride solutions and its modification with alcohols

    SciTech Connect

    Bogacki, M.B.; Jakubiak, A.; Szymanowski, J.; Cote, G.

    1997-03-01

    Dipentyl pyridinedicarboxylates (denoted hereafter as L) with different positions of the ester groups were synthesized and used for copper(II) extraction from chloride solutions containing up to 10 mol/L Cl{sup {minus}}. The effect of decanol addition on copper extraction was studied. A molecular modeling technique was used to estimate the structures of extractants, copper complexes, and associates with alcohol. It was found that the ability of pyridinecarboxylates to extract copper depends on the aqueous phase composition and the position of the ester groups in the pyridine ring. All the investigated compounds except dipentyl pyridine-2,6-dicarboxylate extract copper(II) by formation of CuCl{sub 2}L{sub 2} complexes. Dipentyl pyridine-2,6-dicarboxylate forms another type of complex, probably CuCl{sub 2}L. However, this compound is not suitable for copper extraction as its copper complex precipitates. Dipentyl pyridine-3,5-dicarboxylate was found to be the most suitable extractant among the various compounds listed. Finally it is shown that the possibilities to modify the extraction ability of pyridinecarboxylates with a hydrophobic alcohol such as decanol are relatively weak. Some enhancement was, however, observed when 20% of decanol was added to the organic phase containing dipentyl pyridine-3,5-dicarboxylate.

  10. Assessment of the Polychlorinated Biphenyl (PCB) Occurrence in Copper Sulfates and the Influential Role of PCB Levels on Grapes

    PubMed Central

    Li, Xiaomin; Su, Xiaoou

    2015-01-01

    Copper sulfates (CuSO4) are widely used as the primary component of fungicides in the grape industry. The agricultural-grade CuSO4 that we collected from Chinese nationwide markets were found to be contaminated by polychlorinated dibenzo-p-dioxins and dibenzofurans and high levels of polychlorinated biphenyls (Σ19PCBs: 0.32~9.51 ng/g). In the following research, we studied the impact of CuSO4 application on PCB levels in grape products through a field experiment, and conducted a national survey to speculate the role that CuSO4 played on the occurrence of PCB in grapes. In the field experiment, an obvious increase of PCBs in grape leaves (from 174 to 250 pg/g fw) was observed after Bordeaux mixture (the main component of which is CuSO4) application. As to the main PCB congener in CuSO4, the most toxic CB 126 (toxic equivalency factor = 0.1) also increased in grape peels (from 1.66 to 2.93 pg/g fw) after pesticide spray. Both the correlation study and the principal component analysis indicated that environmental factors were dominant PCB contributors to grapes, and grapes from e-waste dismantling area containing the highest PCBs also proved the notion. It is worth noting that this report describes the first research examining PCBs in CuSO4 and its influence on agricultural products to date. PMID:26658158

  11. Assessment of the Polychlorinated Biphenyl (PCB) Occurrence in Copper Sulfates and the Influential Role of PCB Levels on Grapes.

    PubMed

    Li, Xiaomin; Su, Xiaoou

    2015-01-01

    Copper sulfates (CuSO4) are widely used as the primary component of fungicides in the grape industry. The agricultural-grade CuSO4 that we collected from Chinese nationwide markets were found to be contaminated by polychlorinated dibenzo-p-dioxins and dibenzofurans and high levels of polychlorinated biphenyls (Σ19PCBs: 0.32~9.51 ng/g). In the following research, we studied the impact of CuSO4 application on PCB levels in grape products through a field experiment, and conducted a national survey to speculate the role that CuSO4 played on the occurrence of PCB in grapes. In the field experiment, an obvious increase of PCBs in grape leaves (from 174 to 250 pg/g fw) was observed after Bordeaux mixture (the main component of which is CuSO4) application. As to the main PCB congener in CuSO4, the most toxic CB 126 (toxic equivalency factor = 0.1) also increased in grape peels (from 1.66 to 2.93 pg/g fw) after pesticide spray. Both the correlation study and the principal component analysis indicated that environmental factors were dominant PCB contributors to grapes, and grapes from e-waste dismantling area containing the highest PCBs also proved the notion. It is worth noting that this report describes the first research examining PCBs in CuSO4 and its influence on agricultural products to date. PMID:26658158

  12. Real-time cell analysis: sensitivity of different vertebrate cell cultures to copper sulfate measured by xCELLigence(®).

    PubMed

    Rakers, S; Imse, F; Gebert, M

    2014-10-01

    In this study, we report the use of a real-time cell analysis (RTCA) test system, the xCELLigence(®) RTCA, as efficient tool for a fast cytotoxicity analysis and comparison of four different vertebrate cell cultures. This new dynamic real-time monitoring and impedance-based assay allows for a combined measurement of cell adhesion, spreading and proliferation. Cell cultures were obtained from mouse, rat, human and fish, all displaying a fibroblast-like phenotype. The measured impedance values could be correlated to characteristic cell culture behaviours. In parallel, relative cytotoxicity of a commonly used but due to its very good water solubility highly hazardous pesticide, copper sulfate, was evaluated under in vitro conditions through measurements of cell viability by classical end-point based assays MTT and PrestoBlue(®). Cell line responses in terms of viability as measured by these three methods were variable between the fish skin cells and cells from higher vertebrates and also between the three methods. The advantage of impedance-based measurements is mainly based on the continuous monitoring of cell responses for a broad range of different cells, including fish cells. PMID:25001081

  13. 1D μ-glycine-briged copper (II) chain in complex [Cu(μ-Gly)Im(ClO4)]n and ferromagnetic interactions among copper (II)

    NASA Astrophysics Data System (ADS)

    Pan, Lu; Lv, Xue-Chuan; Luo, Guan-Hua; Gao, Xiao-Han; Tan, Zhi-Cheng

    2016-12-01

    Complex [Cu(μ-Gly)Im(ClO4)]n(Im = imidazole, and Gly = glycine) with μ-glycine-briged copper (II) chain, containing six-coordination distorted elongated octahedron, was synthesized and characterized. The complex belongs to space group P 21/c measured by X-ray single crystal diffraction. In the cluster, each Cu2+ ion are six-coordination by one nitrogen atom and two oxygen atoms of glycine, one nitrogen atoms of imidazole, and two of oxygen atoms of two perchlorate. Each Cu2+ ion has an N2O4 donor set, which forms the distorted elongated octahedron due to the Jahn-Teller (JT) effect. The magnetic and thermodynamic properties were researched. Magnetic susceptibilities of the complex showed that ferromagnetic interactions occurred between the Cu (II) atoms. The Curie-Weiss constant C = 0.565 cm3 K·mol-1 and the Weiss constant θ = 1.0585 K were given by the Curie-Weiss law The ferromagnetic nature of the interaction could be deduced as the exchange pathway of Cusbnd Osbnd Csbnd Osbnd Cu, which involved an equatorial position at one copper (II) ion and an axial position of the nearest copper (II). The complex decomposed from 511 to 538 K as two steps.

  14. Toxic effects of copper on photosystem II of spinach chloroplasts

    SciTech Connect

    Hsu, Bandar; Lee, Jeeyau )

    1988-05-01

    The room temperature fluorescence induction of chloroplasts was utilized as a probe to locate the site of inhibition on PSII by copper. It was found that, while the initial fluorescence yield was hardly affected, the variable fluorescence yield was lowered without significant change in its kinetics. Addition of DCMU, or abolishing oxygen evolution capability by Tris treatment, did not alter this basic inhibition pattern. Copper was also found to lower the fluorescence yield of chloroplasts treated with linolenic acid which inhibited the secondary electron transport on both oxidizing and reducing sides of PSII. The data indicate that copper adversely affects the primary change separation at the PSII reaction center. We suggest that the inhibition is due to creation of a lesion close to the reaction center, leading to increased dissipation of incoming excitation energy to heat.

  15. Selenium isotope fractionation during reduction by Fe(II)-Fe(III) hydroxide-sulfate (green rust)

    USGS Publications Warehouse

    Johnson, T.M.; Bullen, T.D.

    2003-01-01

    We have determined the extent of Se isotope fractionation induced by reduction of selenate by sulfate interlayered green rust (GRSO4), a Fe(II)-Fe(III) hydroxide-sulfate. This compound is known to reduce selenate to Se(0), and it is the only naturally relevant abiotic selenate reduction pathway documented to date. Se reduction reactions, when they occur in nature, greatly reduce Se mobility and bioavailability. Se stable isotope analysis shows promise as an indicator of Se reduction, and Se isotope fractionation by various Se reactions must be known in order to refine this tool. We measured the increase in the 80Se/76Se ratio of dissolved selenate as lighter isotopes were preferentially consumed during reduction by GRSO4. Six different experiments that used GRSO4 made by two methods, with varying solution compositions and pH, yielded identical isotopic fractionations. Regression of all the data yielded an instantaneous isotope fractionation of 7.36 ?? 0.24???. Selenate reduction by GRSO4 induces much greater isotopic fractionation than does bacterial selenate reduction. If selenate reduction by GRSO4 occurs in nature, it may be identifiable on the basis of its relatively large isotopic fractionation. ?? 2003 Elsevier Science Ltd.

  16. Tris(oxamide dioxime-κN,N')nickel(II) sulfate penta-hydrate.

    PubMed

    Belombe, Michel M; Nenwa, Justin; Mbiangue, Yves A; T Fokwa, Boniface P; Dronskowski, Richard

    2008-01-01

    The asymmetric unit of the title compound, [Ni(C(2)H(6)N(4)O(2))(3)]SO(4)·5H(2)O, contains two complex cations, two sulfate anions and ten lattice water mol-ecules. In both independent cations, the central Ni(II) ion adopts a distorted octa-hedral coordination involving six imino N atoms of three bidentate oxamide dioxime ligands. The bulk structure is achieved by a three-dimensional network of O-H⋯O and N-H⋯O hydrogen bonds which inter-link the ionic partners and some water mol-ecules in such a manner that the lattice framework thus formed defines channels parallel to [100]. The other water mol-ecules are lodged inside these channels. Two of the ten water mol-ecules in the asymmetric unit are disordered over three sites, in 0.356 (3):0.324 (5):0.320 (5) and 0.247 (3):0.293 (6):0.460 (6) occupancy ratios, and one O atom of a sulfate ion is also disordered over two sites, with occupancies of 0.621 (5) and 0.379 (5). PMID:21580881

  17. Synthesis and magnetic studies of μ-oxalato-bridged copper(II)-M(III)-copper(II) (M=Cr and Fe) heterotrinuclear complexes

    NASA Astrophysics Data System (ADS)

    Li, Yan-Tuan; Yan, Cui-Wei; Lou, Jian-Fang; Guan, Hua-Shi

    2004-10-01

    Two new μ-oxalato-bridged Cu II2M III-type (M=Cr and Fe) heterotrinuclear complexes have been prepared and identified as [Cu 2Cr(ox) 3(tmen) 2]ClO 4(1) and [Cu 2Fe(ox) 3(tmen) 2]ClO 4(2), where ox represents the oxalato dianions; tmen stands for N,N,N',N'-tetramethylethylenediamine. The two complexes have not yet been isolated in crystalline form suitable for X-ray structure analysis, but based on elemental analyses, molar conductivity and magnetic moment (at room-temperature) measurements, IR, ESR and electronic spectra studies, it is proposed that these complexes have oxalato-bridged structure consisting of two copper(II) ions and a chromium(III) or an iron(III) ion, in which the chromium(III) or iron(III) ion has an octahedral environment, and the two copper(II) ions have a square-planar environment. The two heterotrinuclear complexes were further characterized by variable temperature magnetic susceptibility (4.2-300 K) measurements and the magnetic data have been also used to deduce the indicated heterotrinuclear structure. The results derived from least-squares fit of the experimental data have confirmed that the adjacent copper(II) and chromium(III) ions through the oxalato-bridge in complex (1) are ferromagnetically coupled with the exchange integral J(Cu-Cr)=+10.2 cm -1, while an intramolecular antiferromagnetic coupling is detected between the copper(II) and iron(III) ions through the oxalato-bridge in complex (2) with the exchange integral J(Cu-Fe)=-11.6 cm -1, on the basis of the spin Hamiltonian operator, Ĥ=-2J( ŜCu1· ŜM+ ŜCu2· ŜM) (M=Cr 3+ or Fe 3+).

  18. Human topoisomerase IB is a target of a thiosemicarbazone copper(II) complex.

    PubMed

    Vutey, Venn; Castelli, Silvia; D'Annessa, Ilda; Sâmia, Luciana B P; Souza-Fagundes, Elaine M; Beraldo, Heloisa; Desideri, Alessandro

    2016-09-15

    The human topoisomerase IB inhibition and the antiproliferative activity of 3-(4-bromophenyl)-1-pyridin-2-ylprop-2-en-1-one thiosemicarbazone HPyCT4BrPh alone and its copper(II) complex [Cu(PyCT4BrPh)Cl] was investigated. [Cu(PyCT4BrPh)Cl] inhibits both the DNA cleavage and religation step of the enzyme, whilst the ligand alone does not display any effect. In addition we show that coordination to copper(II) improves the cytotoxicity of HPyCT4BrPh against THP-1 leukemia and MCF-7 breast cancer cells. The data indicate that the copper(II) thiosemicarbazone complex may hit human topoisomerase IB and that metal coordination can be useful to improve cytotoxicity of this versatile class of compounds. PMID:27431056

  19. BSA binding and antimicrobial studies of branched polyethyleneimine-copper(II)bipyridine/phenanthroline complexes

    NASA Astrophysics Data System (ADS)

    Vignesh, Gopalaswamy; Arunachalam, Sankaralingam; Vignesh, Sivanandham; James, Rathinam Arthur

    2012-10-01

    The interaction of two water soluble branched polyethyleneimine-copper(II) complexes containing bipyridine/phenanthroline with bovine serum albumin (BSA) was studied by, UV-Visible absorption, fluorescence, lifetime measurements and circular dichroism spectroscopic techniques. The polymer-copper(II) complexes strongly quench the intrinsic fluorescence of BSA is the static quenching mechanism through hydrogen bonds and van der Waal's attraction. The distance r, between the BSA and the complexes seems to be less than 2 nm indicating that the energy transfer between the donor and acceptor occurs with high probability. Synchronous fluorescence studies indicate the binding of polymer-copper(II) complexes with BSA mostly changes the polarity around tryptophan residues rather than tyrosine residues. The circular dichroism studies indicate that the binding has induced considerable amount of conformational changes in the protein. The complexes also show some antibacterial and antifungal properties.

  20. Synthesis, spectral, photolysis and electrochemical studies of mononuclear copper(II) complex with a new asymmetric tetradentate ligand: Application as copper nanoparticle precursor

    NASA Astrophysics Data System (ADS)

    Habibi, Mohammad Hossein; Mikhak, Maryam

    2012-10-01

    A copper(II) complex with asymmetric tetradentate Schiff base ligand, obtained by the single condensation of 1,2-diaminopropane with 2-hydroxy-5-methoxy benzaldehyde was prepared. The ligand and complex were characterized by their IR, UV-Vis, FT-IR, NMR spectra and CV. Crystal structures of the mononuclear copper complex have been obtained by X-ray diffraction studies which revealed to be distorted square planner coordination geometry. The spectral data confirm coordination of ligand to copper ion center. The redox properties of complex at different scan rates exhibit grossly similar features consisting of an electrochemically pseudo-reversible Cu(II)/Cu(I) reduction at ca. -0.97 V and pseudo-reversible Cu(I)/Cu(II) oxidation at ca. -0.81 V. The copper nanoparticles with average size of 73 nm were formed by thermal reduction of copper complex in the presence of triphenylphosphine.

  1. Calorimetric and spectroscopic studies of solutions of copper(II) and nickel(II) nitrates in water-carbamide mixtures at 298.15 K

    NASA Astrophysics Data System (ADS)

    Vandyshev, V. N.

    2008-06-01

    The heat effects of mixing aqueous solutions of copper(II) and nickel(II) nitrates with water-carbamide mixtures were measured on an isothermic-shell calorimeter in the amide concentration region. An increase in the concentration of carbamide strengthened solvation in copper(II) salt solutions to a greater extent. The electronic absorption spectra were recorded at fixed concentrations of the electrolytes to find that the solvation of copper(II) and nickel(II) nitrates in water-carbamide mixtures involved intrasphere and predominantly outer-sphere cation-solvent interactions, respectively.

  2. Static renewal tests using Anodonta imbecillis (freshwater mussels). Anodonta imbecillis copper sulfate reference toxicant/food test, Clinch River-Environmental Restoration Program (CR-ERP)

    SciTech Connect

    Simbeck, D.J.

    1993-12-31

    Reference toxicant testing using juvenile freshwater mussels was conducted as part of the CR-ERP biomonitoring study of Clinch River sediments to assess the sensitivity of test organisms and the overall performance of the test. Tests were conducted using moderately hard synthetic water spiked with known concentrations of copper as copper sulfate. Two different foods, phytoplankton and YCT-Selenastrum (YCT-S), were tested in side by side tests to compare food quality. Toxicity testing of copper sulfate reference toxicant was conducted from July 6--15, 1993. The organisms used for testing were juvenile fresh-water mussels (Anodonta imbecillis). Although significant reduction in growth, compared to the phytoplankton control, was seen in all treatments, including the YCT-S Control, the consequence of this observation has not been established. Ninety-day testing of juvenile mussels exhibited large variations in growth within treatment and replicate groups. Attachments to this report include: Toxicity test bench sheets and statistical analyses; and Copper analysis request and results.

  3. COPPER

    EPA Science Inventory

    The report is a review of current knowledge of the distribution of copper in the environment and living things. Metabolism and the effects of copper in the biosphere are also considered. Copper compounds are common and widely distributed in nature. They are also extensively mined...

  4. The effect of copper on the mRNA expression profile of xenobiotic-metabolizing enzymes in cultured rat H4-II-E cells.

    PubMed

    Darwish, Wageh Sobhy; Ikenaka, Yoshinori; Nakayama, Shouta; Ishizuka, Mayumi

    2014-05-01

    Copper (Cu(2+)) is an essential element that plays important roles in physiological functions of the body. However, high Cu(2+) levels can have toxic implications. This study aims to investigate the constitutive response to Cu(2+) exposure of xenobiotic-metabolizing enzymes in cultured rat liver (H4-II-E) cell lines. Rat cells were exposed to copper sulfate (0-500 μM) for 24 h. The effects of Cu(2+) on the messenger RNA (mRNA) expressions of phase I and II enzymes and regulatory elements were examined using real-time PCR. Metallothionein mRNA expression was induced in a dose-dependent manner after treatment with Cu(2+). mRNA expressions of phase I enzymes such as cytochrome P450 1A1 and 1A2 (CYP1A1 and CYP1A2) were slightly induced after exposure to low concentrations of Cu(2+); however, CYP1A1 and CYP1A2 mRNA expressions were significantly downregulated at higher Cu(2+) concentrations. These effects corresponded with expression of aryl hydrocarbon receptor mRNA. The mRNA expressions of phase II enzymes were reduced upon exposure to Cu(2+). In conclusion, phase I and II enzyme expressions were significantly modulated upon Cu(2+) exposure. These results indicated that Cu(2+) exposure had toxicological implications for cultured H4-II-E cells. PMID:24599699

  5. Structural specifics of light-induced metastable states in copper(II)-nitroxide molecular magnets.

    PubMed

    Barskaya, I Yu; Veber, S L; Fokin, S V; Tretyakov, E V; Bagryanskaya, E G; Ovcharenko, V I; Fedin, M V

    2015-12-28

    Although light-induced magnetostructural switching in copper(II)-nitroxide molecular magnets Cu(hfac)2L(R) has been known for several years, structural characterization of metastable photoinduced states has not yet been accomplished due to significant technical demands. In this work we apply, for the first time, variable-temperature FTIR spectroscopy with photoexcitation to investigate the structural specifics of light-induced states in the Cu(hfac)2L(R) family represented by (i) Cu(hfac)2L(Me) comprising two-spin copper(II)-nitroxide clusters, and (ii) Cu(hfac)2L(Pr) comprising three-spin nitroxide-copper(II)-nitroxide clusters. The light-induced state of Cu(hfac)2L(Me) manifests the same set of vibrational bands as the corresponding thermally-induced state, implying their similar structures. For the second compound Cu(hfac)2L(Pr), the coordination environment of copper(II) is similar in light- and thermally-induced states, but distinct differences are found for packing of the peripheral n-propyl substituent of nitroxide. Thus, generally the structures of the corresponding thermally- and light-induced states in molecular magnets Cu(hfac)2L(R) might differ, and FTIR spectroscopy provides a useful approach for revealing and elucidating such differences. PMID:26571045

  6. Heparan Sulfate Proteoglycans Promote Telomerase Internalization and MHC Class II Presentation on Dendritic Cells.

    PubMed

    Galaine, Jeanne; Kellermann, Guillaume; Guillaume, Yves; Boidot, Romain; Picard, Emilie; Loyon, Romain; Queiroz, Lise; Boullerot, Laura; Beziaud, Laurent; Jary, Marine; Mansi, Laura; André, Claire; Lethier, Lydie; Ségal-Bendirdjian, Evelyne; Borg, Christophe; Godet, Yann; Adotévi, Olivier

    2016-09-01

    Telomerase is a prototype-shared tumor Ag and represents an attractive target for anticancer immunotherapy. We have previously described promiscuous and immunogenic HLA-DR-restricted peptides derived from human telomerase reverse transcriptase (hTERT) and referred as universal cancer peptide (UCP). In nonsmall cell lung cancer, the presence of spontaneous UCP-specific CD4 T cell responses increases the survival of chemotherapy-responding patients. However, the precise mechanisms of hTERT's uptake, processing, and presentation on MHC-II molecules to stimulate CD4 T cells are poorly understood. In this work, by using well-characterized UCP-specific CD4 T cell clones, we showed that hTERT processing and presentation on MHC-II involve both classical endolysosomal and nonclassical cytosolic pathways. Furthermore, to our knowledge, we demonstrated for the first time that hTERT's internalization by dendritic cells requires its interaction with surface heparan sulfate proteoglycans. Altogether, our findings provide a novel mechanism of tumor-specific CD4 T cell activation and will be useful for the development of novel cancer immunotherapies that harness CD4 T cells. PMID:27481844

  7. Delineation of vagal emetic pathways: intragastric copper sulfate-induced emesis and viral tract tracing in musk shrews.

    PubMed

    Horn, Charles C; Meyers, Kelly; Lim, Audrey; Dye, Matthew; Pak, Diana; Rinaman, Linda; Yates, Bill J

    2014-03-01

    Signals from the vestibular system, area postrema, and forebrain elicit nausea and vomiting, but gastrointestinal (GI) vagal afferent input arguably plays the most prominent role in defense against food poisoning. It is difficult to determine the contribution of GI vagal afferent input on emesis because various agents (e.g., chemotherapy) often act on multiple sensory pathways. Intragastric copper sulfate (CuSO4) potentially provides a specific vagal emetic stimulus, but its actions are not well defined in musk shrews (Suncus murinus), a primary small animal model used to study emesis. The aims of the current study were 1) to investigate the effects of subdiaphragmatic vagotomy on CuSO4-induced emesis and 2) to conduct preliminary transneuronal tracing of the GI-brain pathways in musk shrews. Vagotomy failed to inhibit the number of emetic episodes produced by optimal emetic doses of CuSO4 (60 and 120 mg/kg ig), but the effects of lower doses were dependent on an intact vagus (20 and 40 mg/kg). Vagotomy also failed to affect emesis produced by motion (1 Hz, 10 min) or nicotine administration (5 mg/kg sc). Anterograde transport of the H129 strain of herpes simplex virus-1 from the ventral stomach wall identified the following brain regions as receiving inputs from vagal afferents: the nucleus of the solitary tract, area postrema, and lateral parabrachial nucleus. These data indicate that the contribution of vagal pathways to intragastric CuSO4-induced emesis is dose dependent in musk shrews. Furthermore, the current neural tracing data suggest brain stem anatomical circuits that are activated by GI signaling in the musk shrew. PMID:24430885

  8. The Unusual Colour of Copper Deposited on a Graphite Electrode in an Aqueous Solution of CuSO[subscript 4

    ERIC Educational Resources Information Center

    Petrusevski, Vladimir M.; Stojanovska, Marina

    2010-01-01

    The colour of the copper layer deposited on a graphite electrode during electrolysis of an aqueous solution of copper(II) sulfate looks whitish-grey when inspected in situ. Taking the electrode out of the solution reveals the familiar orange-red colour of deposited copper. The explanation is found in terms of the almost ideal complementary colours…

  9. Spectroscopic characterization, antioxidant and antitumour studies of novel bromo substituted thiosemicarbazone and its copper(II), nickel(II) and palladium(II) complexes.

    PubMed

    Jagadeesh, M; Lavanya, M; Kalangi, Suresh K; Sarala, Y; Ramachandraiah, C; Varada Reddy, A

    2015-01-25

    A new, slightly distorted octahedral complex of copper(II), square planar complexes of nickel(II) and palladium(II) with 2,4'-dibromoacetophenone thiosemicarbazone (DBAPTSC) are synthesized. The ligand and the complexes are characterized by FT-IR, FT-Raman, powder X-ray diffraction studies. The IR and Raman data are correlated for the presence of the functional groups which specifically helped in the confirmation of the compounds. In addition, the free ligand is unambiguously characterized by (1)H and (13)C NMR spectroscopy while the copper(II) complex is characterized by electron paramagnetic resonance spectroscopy (EPR). The g values for the same are found to be 2.246 (g1), 2.012 (g2) and 2.005 (g3) which suggested rhombic distortions. The HOMO-LUMO band gap calculations for these compounds are found to be in between 0.5 and 4.0 eV and these compounds are identified as semiconducting materials. The synthesized ligand and its copper(II), nickel(II) and palladium(II) complexes are subjected to antitumour activity against the HepG2 human hepatoblastoma cell lines. Among all the compounds, nickel(II) complex is found to exert better antitumour activity with 57.6% of cytotoxicity. PMID:25064500

  10. Spectroscopic characterization, antioxidant and antitumour studies of novel bromo substituted thiosemicarbazone and its copper(II), nickel(II) and palladium(II) complexes

    NASA Astrophysics Data System (ADS)

    Jagadeesh, M.; Lavanya, M.; Kalangi, Suresh K.; Sarala, Y.; Ramachandraiah, C.; Varada Reddy, A.

    2015-01-01

    A new, slightly distorted octahedral complex of copper(II), square planar complexes of nickel(II) and palladium(II) with 2,4‧-dibromoacetophenone thiosemicarbazone (DBAPTSC) are synthesized. The ligand and the complexes are characterized by FT-IR, FT-Raman, powder X-ray diffraction studies. The IR and Raman data are correlated for the presence of the functional groups which specifically helped in the confirmation of the compounds. In addition, the free ligand is unambiguously characterized by 1H and 13C NMR spectroscopy while the copper(II) complex is characterized by electron paramagnetic resonance spectroscopy (EPR). The g values for the same are found to be 2.246 (g1), 2.012 (g2) and 2.005 (g3) which suggested rhombic distortions. The HOMO-LUMO band gap calculations for these compounds are found to be in between 0.5 and 4.0 eV and these compounds are identified as semiconducting materials. The synthesized ligand and its copper(II), nickel(II) and palladium(II) complexes are subjected to antitumour activity against the HepG2 human hepatoblastoma cell lines. Among all the compounds, nickel(II) complex is found to exert better antitumour activity with 57.6% of cytotoxicity.

  11. PEP-II vacuum system - joining SS flanges to copper beam chambers

    SciTech Connect

    Fetzko, S.; Hoyt, E.; Cummings, U.

    1994-06-01

    Various methods of joining stainless steel flanges to the copper PEP-II high-energy ring vacuum chambers was investigated with regard to metallurgical soundness, reliability, complexity, and cost. The most promising method appears to be direct electron-beam welding.

  12. Redox Activity of Copper(II) Complexes with NSFRY Pentapeptide and Its Analogues

    PubMed Central

    Wiloch, Magdalena Zofia; Wawrzyniak, Urszula Elżbieta; Ufnalska, Iwona; Piotrowski, Grzegorz; Bonna, Arkadiusz; Wróblewski, Wojciech

    2016-01-01

    The influence of cation-π interactions on the electrochemical properties of copper(II) complexes with synthesized pentapeptide C-terminal fragment of Atrial Natriuretic Factor (ANF) hormone was studied in this work. Molecular modeling performed for Cu(II)-NSFRY-NH2 complex indicated that the cation-π interactions between Tyr and Cu(II), and also between Phe-Arg led to specific conformation defined as peptide box, in which the metal cation is isolated from the solvent by peptide ligand. Voltammetry experiments enabled to compare the redox properties and stability of copper(II) complexes with NSFRY-NH2 and its analogues (namely: NSFRA-NH2, NSFRF-NH2, NSAAY-NH2, NSAAA-NH2, AAAAA-NH2) as well as to evaluate the contribution of individual amino acid residues to these properties. The obtained results led to the conclusion, that cation-π interactions play a crucial role in the effective stabilization of copper(II) complexes with the fragments of ANF peptide hormone and therefore could control the redox processes in other metalloproteins. PMID:27517864

  13. Photochemical oxidation of coals and some selected model compounds by using copper(II) chloride

    SciTech Connect

    Yilmaz, M.

    1999-12-01

    The H-donor ability of different rank coals was examined by using a copper(II)chloride-acetonitrile system as the dehydrogenator. A bituminous coal and two lignites were irradiated in the UV in the presence of copper(II)chloride in acetonitrile. The coal was dehydrogenated while the Cu(II) was reduced to CU(I). Considerable amounts of aliphatic or alicyclic hydrogen were removed from the coals. In the process, while the oxygen contents of coals do not increase, more condensed aromatic products occur. It was concluded that lignites are better reducing agents than bituminous coals. A photooxidation mechanism is proposed on the basis of the model compound reaction. Photooxidation of alcohols (ethanol, 2-propanol, benzyl alcohol, 4-hydroxybenzyl alcohol, and diphenyl carbinol), a hydroaromatic compound (tetrahydronaphthalene), and an aromatic ether (dibenzyl ether) was performed under similar reaction conditions.

  14. Reduction of phenanthroline complexes of copper (II) by alcohols in alkaline aqueous media

    SciTech Connect

    Chudaev, V.V.; Rudakov, E.S.; Tret'ya, V.P.

    1986-09-01

    The method of electron spectrophotometry has been used to investigate the kinetics of reducing phenanthroline complexes of copper (II) by alcohols in aqeous alkaline solutions in the absence of oxygen at 365/sup 0/K. The reaction is first order with respect to Cu(II) and second order with respect to alcohol. The rate of reduction of Cu(II) by alcohols increases sharply in the presence of the /ETA/ atom acceptor 2,2,6,6-tetramethylpiperidene-nitroxyl radical. The kinetic isotropic effect (k /SUB H/ /k /SUB D/ approx. = 2) indicates rupture of the C - H bond at the ..cap alpha..-carbon atom. The formation of a copper complex with the alkoxyl radical as an intermediate particle detaching an /ETA/ atom from the alcohol molecule has been investigated.

  15. Immobilization of Hg(II) by Coprecipitation in Sulfate-Cement Systems

    PubMed Central

    2012-01-01

    Uptake and molecular speciation of dissolved Hg during formation of Al- or Fe-ettringite-type and high-pH phases were investigated in coprecipitation and sorption experiments of sulfate-cement treatments used for soil and sediment remediation. Ettringite and minor gypsum were identified by XRD as primary phases in Al systems, whereas gypsum and ferrihydrite were the main products in Hg–Fe precipitates. Characterization of Hg–Al solids by bulk Hg EXAFS, electron microprobe, and microfocused-XRF mapping indicated coordination of Hg by Cl ligands, multiple Hg and Cl backscattering atoms, and concentration of Hg as small particles. Thermodynamic predictions agreed with experimental observations for bulk phases, but Hg speciation indicated lack of equilibration with the final solution. Results suggest physical encapsulation of Hg as a polynuclear chloromercury(II) salt in ettringite as the primary immobilization mechanism. In Hg–Fe solids, structural characterization indicated Hg coordination by O atoms only and Fe backscattering atoms that is consistent with inner-sphere complexation of Hg(OH)20 coprecipitated with ferrihydrite. Precipitation of ferrihydrite removed Hg from solution, but the resulting solid was sufficiently hydrated to allow equilibration of sorbed Hg species with the aqueous solution. Electron microprobe XRF characterization of sorption samples with low Hg concentration reacted with cement and FeSO4 amendment indicated correlation of Hg and Fe, supporting the interpretation of Hg removal by precipitation of an Fe(III) oxide phase. PMID:22594782

  16. Immobilization of Hg(II) by coprecipitation in sulfate-cement systems.

    PubMed

    Serrano, Susana; Vlassopoulos, Dimitri; Bessinger, Brad; O'Day, Peggy A

    2012-06-19

    Uptake and molecular speciation of dissolved Hg during formation of Al- or Fe-ettringite-type and high-pH phases were investigated in coprecipitation and sorption experiments of sulfate-cement treatments used for soil and sediment remediation. Ettringite and minor gypsum were identified by XRD as primary phases in Al systems, whereas gypsum and ferrihydrite were the main products in Hg-Fe precipitates. Characterization of Hg-Al solids by bulk Hg EXAFS, electron microprobe, and microfocused-XRF mapping indicated coordination of Hg by Cl ligands, multiple Hg and Cl backscattering atoms, and concentration of Hg as small particles. Thermodynamic predictions agreed with experimental observations for bulk phases, but Hg speciation indicated lack of equilibration with the final solution. Results suggest physical encapsulation of Hg as a polynuclear chloromercury(II) salt in ettringite as the primary immobilization mechanism. In Hg-Fe solids, structural characterization indicated Hg coordination by O atoms only and Fe backscattering atoms that is consistent with inner-sphere complexation of Hg(OH)(2)(0) coprecipitated with ferrihydrite. Precipitation of ferrihydrite removed Hg from solution, but the resulting solid was sufficiently hydrated to allow equilibration of sorbed Hg species with the aqueous solution. Electron microprobe XRF characterization of sorption samples with low Hg concentration reacted with cement and FeSO(4) amendment indicated correlation of Hg and Fe, supporting the interpretation of Hg removal by precipitation of an Fe(III) oxide phase. PMID:22594782

  17. Copper(II) ions and the Alzheimer's amyloid-β peptide: Affinity and stoichiometry of binding

    NASA Astrophysics Data System (ADS)

    Tõugu, Vello; Friedemann, Merlin; Tiiman, Ann; Palumaa, Peep

    2014-10-01

    Deposition of amyloid beta (Aβ) peptides into amyloid plaques is the hallmark of Alzheimer's disease. According to the amyloid cascade hypothesis this deposition is an early event and primary cause of the disease, however, the mechanisms that cause this deposition remain elusive. An increasing amount of evidence shows that the interactions of biometals can contribute to the fibrillization and amyloid formation by amyloidogenic peptides. From different anions the copper ions deserve the most attention since it can contribute not only toamyloid formation but also to its toxicity due to the generation of ROS. In this thesis we focus on the affinity and stoichiometry of copper(II) binding to the Aβ molecule.

  18. Antitubercular and fluorescence studies of copper(II) complexes with quinolone family member, ciprofloxacin

    NASA Astrophysics Data System (ADS)

    Kharadi, G. J.

    2011-09-01

    Four new mixed-ligand complexes of Cu(II) with ciprofloxacin (Cip) and uninegative bidentate ligands have been synthesized and characterized. The structure of mixed-ligand complexes was investigated using spectroscopic method, physicochemical and elemental analyses. The fluorescence spectra of complexes show red shift, which may be due to the chelation by the ligands to the metal ion. It enhances ligand ability to accept electrons and decreases the electron transition energy. Antimycobacterial screening of ligand and its copper compound against Mycobacterium tuberculosis shows clear enhancement in the antitubercular activity upon copper complexation.

  19. Experimental investigation on the mechanism of chelation-assisted, copper(II) acetate-accelerated azide-alkyne cycloaddition.

    PubMed

    Kuang, Gui-Chao; Guha, Pampa M; Brotherton, Wendy S; Simmons, J Tyler; Stankee, Lisa A; Nguyen, Brian T; Clark, Ronald J; Zhu, Lei

    2011-09-01

    A mechanistic model is formulated to account for the high reactivity of chelating azides (organic azides capable of chelation-assisted metal coordination at the alkylated azido nitrogen position) and copper(II) acetate (Cu(OAc)(2)) in copper(II)-mediated azide-alkyne cycloaddition (AAC) reactions. Fluorescence and (1)H NMR assays are developed for monitoring the reaction progress in two different solvents, methanol and acetonitrile. Solvent kinetic isotopic effect and premixing experiments give credence to the proposed different induction reactions for converting copper(II) to catalytic copper(I) species in methanol (methanol oxidation) and acetonitrile (alkyne oxidative homocoupling), respectively. The kinetic orders of individual components in a chelation-assisted, copper(II)-accelerated AAC reaction are determined in both methanol and acetonitrile. Key conclusions resulting from the kinetic studies include (1) the interaction between copper ion (either in +1 or +2 oxidation state) and a chelating azide occurs in a fast, pre-equilibrium step prior to the formation of the in-cycle copper(I)-acetylide, (2) alkyne deprotonation is involved in several kinetically significant steps, and (3) consistent with prior experimental and computational results by other groups, two copper centers are involved in the catalysis. The X-ray crystal structures of chelating azides with Cu(OAc)(2) suggest a mechanistic synergy between alkyne oxidative homocoupling and copper(II)-accelerated AAC reactions, in which both a bimetallic catalytic pathway and a base are involved. The different roles of the two copper centers (a Lewis acid to enhance the electrophilicity of the azido group and a two-electron reducing agent in oxidative metallacycle formation, respectively) in the proposed catalytic cycle suggest that a mixed valency (+2 and +1) dinuclear copper species be a highly efficient catalyst. This proposition is supported by the higher activity of the partially reduced Cu(OAc)(2) in

  20. Polynuclear and mixed-ligand complexes of copper(II) and nickel(II) with (1-hydroxyethylidene)bisphosphonic acid

    SciTech Connect

    Amirov, R.R.; Saprykova, Z.A.

    1987-12-20

    The compositions and stabilities of heteronuclear and mixed-ligand copper(II) and nickel(II) (1-hydroxyethylidene)bisphosphonates were determined. Parameters of the compatibility of the ligands and central ions in the complexes were calculated. It was shown that the monoprotonated anion of (1-hydroxyethylidene)bisphosphonic acid is capable of terdentate coordination with the participation of the alcoholic hydroxy group. The acidities of the solutions were determined on a pH-673 meter. The spin-lattice relaxation time was measured on a pulse NMR spectrometer.

  1. Subneurotoxic copper(II)-induced NF-κB-dependent microglial activation is associated with mitochondrial ROS.

    PubMed

    Hu, Zhuqin; Yu, Fengxiang; Gong, Ping; Qiu, Yu; Zhou, Wei; Cui, Yongyao; Li, Juan; Chen, Hongzhuan

    2014-04-15

    Microglia-mediated neuroinflammation and the associated neuronal damage play critical roles in the pathogenesis of neurodegenerative disorders. Evidence shows an elevated concentration of extracellular copper(II) in the brains of these disorders, which may contribute to neuronal death through direct neurotoxicity. Here we explored whether extracellular copper(II) triggers microglial activation. Primary rat microglia and murine microglial cell line BV-2 cells were cultured and treated with copper(II). The content of tumor necrosis factor-α (TNF-α) and nitric oxide in the medium was determined. Extracellular hydrogen peroxide was quantified by a fluorometric assay with Amplex Red. Mitochondrial superoxide was measured by MitoSOX oxidation. At subneurotoxic concentrations, copper(II) treatment induced a dose- and time-dependent release of TNF-α and nitric oxide from microglial cells, and caused an indirect, microglia-mediated neurotoxicity that was blocked by inhibition of TNF-α and nitric oxide production. Copper(II)-initiated microglial activation was accompanied with reduced IкB-α expression as well as phosphorylation and translocation of nuclear factor-κB (NF-κB) p65 and was blocked by NF-κB inhibitors (BAY11-7082 and SC-514). Moreover, copper(II) treatment evoked a rapid release of hydrogen peroxide from microglial cells, an effect that was not affected by NADPH oxidase inhibitors. N-acetyl-cysteine, a scavenger of reactive oxygen species (ROS), abrogated copper(II)-elicited microglial release of TNF-α and nitric oxide and subsequent neurotoxicity. Importantly, mitochondrial production of superoxide, paralleled to extracellular release of hydrogen peroxide, was induced after copper(II) stimulation. Our findings suggest that extracellular copper(II) at subneurotoxic concentrations could trigger NF-κB-dependent microglial activation and subsequent neurotoxicity. NADPH oxidase-independent, mitochondria-derived ROS may be involved in this activation. PMID

  2. Effects of the interrelationship between zinc oxide and copper sulfate on growth performance of early-weaned pigs.

    PubMed

    Smith, J W; Tokach, M D; Goodband, R D; Nelssen, J L; Richert, B T

    1997-07-01

    We conducted four experiments to examine the effects of adding zinc oxide (ZnO) and(or) copper sulfate (CuSO4) to diets for weanling pigs. In Exp. 1 and 2, weanling pigs (initially 5.3 kg and 19 +/- 2 d of age) were fed diets containing 250 ppm of added Cu (CuSO4) and either 110 or 3,110 ppm of added. Zn (ZnO). No differences (P > .10) were observed in either experiment for ADG, ADFI, or feed efficiency (G:F). In Exp. 3,240 pigs (initially 4.45 kg and 15 +/- 2 d of age) were used to determine the interactive effects of added dietary ZnO and(or) CuSO4. Dietary treatments were in a 2 x 2 factorial arrangement; Zn (165 or 3,000 ppm) and Cu (16.5 or 250 ppm) were the main effects. Pigs were fed a high nutrient dense diet from d 0 to 14 after weaning and a less complex diet from d 14 to 28 after weaning, both containing the same mineral fortifications. From d 0 to 14, pigs fed 3,000 ppm Zn, with or without 250 ppm Cu, had improved ADG (P < .01) compared with pigs fed the control (16.5 ppm Cu and 165 ppm Zn) or diets with only added Cu. From d 14 to 28, pigs fed the diet containing 3,000 ppm added Zn, without 250 ppm Cu, had greater ADG than pigs fed the other diets (Zn x Cu interaction, P < .01). In Exp. 4, 264 pigs (initially 4.17 kg and 12 +/- 3 d of age) were fed a high nutrient dense diet supplemented with 3,000 ppm of Zn (ZnO) from d 0 to 14 after weaning. On d 14, pigs were switched to the diets containing experimental mineral levels identical to those of Exp. 3. From d 14 to 28 after weaning, added Zn improved ADG but not when the diet contained 250 ppm Cu (Zn x Cu interaction, P < .05). Feeding 3,000 ppm of Zn from ZnO is a viable means of improving nursery pig performance, but additive responses to growth-promotant levels of CuSO4 (250 ppm Cu) were not observed. PMID:9222843

  3. Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion.

    PubMed

    Holder, Patrick G; Jones, Lesley C; Drake, Penelope M; Barfield, Robyn M; Bañas, Stefanie; de Hart, Gregory W; Baker, Jeanne; Rabuka, David

    2015-06-19

    To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of aerobic formylglycine-generating enzyme (FGE) in good yield. We then optimized the FGE biocatalytic reaction conditions for conversion of cysteine to formylglycine in aldehyde tags on intact monoclonal antibodies. During the development of these conditions, we discovered that pretreating FGE with copper(II) is required for high turnover rates and yields. After further investigation, we confirmed that both aerobic prokaryotic (Streptomyces coelicolor) and eukaryotic (Homo sapiens) FGEs contain a copper cofactor. The complete kinetic parameters for both forms of FGE are described, along with a proposed mechanism for FGE catalysis that accounts for the copper-dependent activity. PMID:25931126

  4. Catalytic wet oxidation of thiocyanate with homogeneous copper(II) sulphate catalyst.

    PubMed

    Collado, Sergio; Laca, Adriana; Díaz, Mario

    2010-05-15

    The wet oxidation of thiocyanate has been investigated in a semi-batch reactor at temperatures between 423 and 473 K and pressures between 6.1 x 10(3) and 1.0 x 10(4)kPa in the presence of copper(II) sulphate as catalyst. The effects of copper concentration, initial thiocyanate concentration, pressure and temperature on the reaction rate were analyzed and the main products of reaction were identified. A kinetic model for the Cu-catalyzed reaction is here proposed, including temperature, oxygen concentration, and the reduction of Cu(2+) to Cu(+) that gives an accurate prediction of the oxidation process under the assayed conditions. A mechanistic model based on the formation of a transition complex between a copper cation and two thiocyanate anions has been proposed for the catalytic wet oxidation. PMID:20045245

  5. Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion

    PubMed Central

    Holder, Patrick G.; Jones, Lesley C.; Drake, Penelope M.; Barfield, Robyn M.; Bañas, Stefanie; de Hart, Gregory W.; Baker, Jeanne; Rabuka, David

    2015-01-01

    To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of aerobic formylglycine-generating enzyme (FGE) in good yield. We then optimized the FGE biocatalytic reaction conditions for conversion of cysteine to formylglycine in aldehyde tags on intact monoclonal antibodies. During the development of these conditions, we discovered that pretreating FGE with copper(II) is required for high turnover rates and yields. After further investigation, we confirmed that both aerobic prokaryotic (Streptomyces coelicolor) and eukaryotic (Homo sapiens) FGEs contain a copper cofactor. The complete kinetic parameters for both forms of FGE are described, along with a proposed mechanism for FGE catalysis that accounts for the copper-dependent activity. PMID:25931126

  6. Copper(II)-chelating homocarnosine glycoconjugate as a new multifunctional compound.

    PubMed

    Grasso, Giuseppa I; Arena, Giuseppe; Bellia, Francesco; Rizzarelli, Enrico; Vecchio, Graziella

    2014-02-01

    Homocarnosine is an endogenous dipeptide distributed in cerebral regions and cerebrospinal fluid. Homocarnosine may serve as an antioxidant, free radical scavenger, neurotransmitter, buffering system and metal chelating agent, especially for copper(II) and zinc(II). The homeostasis of homocarnosine is regulated by carnosinases; the serum-circulating isoform of these metallodipeptidases partially hydrolyses homocarnosine in the blood. The enzyme activity is also inhibited by homocarnosine itself in a dose-dependent manner. We synthesized a new multifunctional homocarnosine derivative with trehalose, a disaccharide that possesses several beneficial properties, among which the inhibition of protein aggregation (i.e. Aβ amyloid and polyglutamine proteins) involved in widespread neurodegenerative disorders. We studied the copper(II) binding features of the new conjugate by means of potentiometric and spectroscopic techniques (UV-visible and circular dichroism) and the superoxide dismutase-like activity of the copper(II) complexes with homocarnosine and its trehalose conjugate was evaluated. The inhibitory effect of the new homocarnosine derivative on the carnosinase activity and its effects on Aβ aggregation were also investigated. PMID:24246303

  7. The Tachykinin Peptide Neurokinin B Binds Copper Forming an Unusual [CuII(NKB)2] Complex and Inhibits Copper Uptake into 1321N1 Astrocytoma Cells

    PubMed Central

    2013-01-01

    Neurokinin B (NKB) is a member of the tachykinin family of neuropeptides that have neuroinflammatory, neuroimmunological, and neuroprotective functions. In a neuroprotective role, tachykinins can help protect cells against the neurotoxic processes observed in Alzheimer’s disease. A change in copper homeostasis is a clear feature of Alzheimer’s disease, and the dysregulation may be a contributory factor in toxicity. Copper has recently been shown to interact with neurokinin A and neuropeptide γ and can lead to generation of reactive oxygen species and peptide degradation, which suggests that copper may have a place in tachykinin function and potentially misfunction. To explore this, we have utilized a range of spectroscopic techniques to show that NKB, but not substance P, can bind CuII in an unusual [CuII(NKB)2] neutral complex that utilizes two N-terminal amine and two imidazole nitrogen ligands (from each molecule of NKB) and the binding substantially alters the structure of the peptide. Using 1321N1 astrocytoma cells, we show that copper can enter the cells and subsequently open plasma membrane calcium channels but when bound to neurokinin B copper ion uptake is inhibited. This data suggests a novel role for neurokinin B in protecting cells against copper-induced calcium changes and implicates the peptide in synaptic copper homeostasis. PMID:23875773

  8. The structure of amorphous bulk and silica-supported copper(II) hydroxides

    SciTech Connect

    Kriventsov, V.V.; Kochubey, D.I.; Elizarova, G.L.; Matvienko, L.G.; Parmon, V.N.

    1999-07-01

    Determination of the structure of surface hydroxocompounds is one of the most delicate areas of environmental chemistry, geochemistry, and catalysis. In nature, these compounds are formed everywhere, mostly by absorption of multicharged metal cations on different soil constitutents from water solutions. The data obtained show that at pH 7 copper(II) ions are adsorbed on a SiO{sub 2} surface as polymeric species of hydroxide nature. The structure of these species is similar to that of the bulk amorphous copper hydroxide. The amorphous state of supported Cu(OH){sub 2} is caused by a small (ca. 11 {angstrom}) size of the surface particles. In contrast, the overstoichiometric water molecules seem to act as ``amorphizers`` of the bulk copper hydroxide. The structures of the bulk and dispersed amorphous copper(II) hydroxide were determined. The amorphous Cu(OH){sub 2} has a layered structure close to the structure of the crystalline hydroxide, but the layers in the amorphous hydroxide are shifted toward one another approximately for {1/4} of the c period of the lattice.

  9. Intercalation of organic molecules in 2D copper (II) nitroprusside: Intermolecular interactions and magnetic properties

    SciTech Connect

    Osiry, H.; Cano, A.; Lemus-Santana, A.A.; Rodríguez, A.; Carbonio, R.E.; Reguera, E.

    2015-10-15

    This contribution discusses the intercalation of imidazole and its 2-ethyl derivative, and pyridine in 2D copper nitroprusside. In the interlayer region, neighboring molecules remain interacting throu gh their dipole and quadrupole moments, which supports the solid 3D crystal structure. The crystal structure of this series of intercalation compounds was solved and refined from powder X-ray diffraction patterns complemented with spectroscopic information. The intermolecular interactions were studied from the refined crystal structures and low temperature magnetic measurements. Due to strong attractive forces between neighboring molecules, the resulting π–π cloud overlapping enables the ferromagnetic coupling between metal centers on neighboring layers, which was actually observed for the solids containing imidazole and pyridine as intercalated molecules. For these two solids, the magnetic data were properly described with a model of six neighbors. For the solid containing 2-ethylimidazole and for 2D copper nitroprusside, a model of four neighbors in a plane is sufficient to obtain a reliable data fitting. - Highlights: • Intercalation of organic molecules in 2D copper (II) nitroprusside. • Molecular properties of intercalation compounds of 2D copper (II) nitroprusside. • Magnetic properties of hybrid inorganic–organic solids. • Hybrid inorganic–organic 3D framework.

  10. The effect of copper(II), iron(II) sulphate, and vitamin C combinations on the weak antimicrobial activity of (+)-catechin against Staphylococcus aureus and other microbes.

    PubMed

    Holloway, Andrew C; Mueller-Harvey, Irene; Gould, Simon W J; Fielder, Mark D; Naughton, Declan P; Kelly, Alison F

    2012-12-01

    Few attempts have been made to improve the activity of plant compounds with low antimicrobial efficacy. (+)-Catechin, a weak antimicrobial tea flavanol, was combined with putative adjuncts and tested against different species of bacteria. Copper(II) sulphate enhanced (+)-catechin activity against Pseudomonas aeruginosa but not Staphylococcus aureus, Proteus mirabilis or Escherichia coli. Attempts to raise the activity of (+)-catechin against two unresponsive species, S. aureus and E. coli, with iron(II) sulphate, iron(III) chloride, and vitamin C, showed that iron(II) enhanced (+)-catechin against S. aureus, but not E. coli; neither iron(III) nor combined iron(II) and copper(II), enhanced (+)-catechin activity against either species. Vitamin C enhanced copper(II) containing combinations against both species in the absence of iron(II). Catalase or EDTA added to active samples removed viability effects suggesting that active mixtures had produced H(2)O(2)via the action of added metal(II) ions. H(2)O(2) generation by (+)-catechin plus copper(II) mixtures and copper(II) alone could account for the principal effect of bacterial growth inhibition following 30 minute exposures as well as the antimicrobial effect of (+)-catechin-iron(II) against S. aureus. These novel findings about a weak antimicrobial flavanol contrast with previous knowledge of more active flavanols with transition metal combinations. Weak antimicrobial compounds like (+)-catechin within enhancement mixtures may therefore be used as efficacious agents. (+)-Catechin may provide a means of lowering copper(II) or iron(II) contents in certain crop protection and other products. PMID:23138340

  11. Electron-nuclear double resonance on copper (II) tetraimidazole

    NASA Astrophysics Data System (ADS)

    Van Camp, Harlan L.; Sands, Richard H.; Fee, James A.

    1981-09-01

    We have investigated the electron-nuclear double resonance (ENDOR) from frozen aqueous solutions of 65Cu++(imidazole)4, 65Cu++ (imidazole-15N)4, and 65Cu++(imidazole-Dn)4, where n = 1, 2, 3, and 4 for selectively deuterated imidazole. We have observed ENDOR associated with the imidazole protons and the two imidazole nitrogens. The selective deuteration has allowed us to attempt identification of the weakly coupled protons responsible for the ENDOR spectrum, and a comparison of the overall line shape of that spectrum taken at two extreme points of the EPR spectrum suggests that some of the imidazole planes are tilted with respect to the plane of the complex. The ENDOR arising from the nitrogen nearest the copper is primarily isotropic with A(g⊥) = 41.6±1.5 MHz and A(g∥) = 39.8±1.5 MHz. The resonance shows little structure and seems consistent with a picture that requires some inequivalence among the various imidazoles. The remote nitrogen ENDOR reveals both hyperfine and quadrupole effects with approximately isotropic A(14N) = 1.79 MHz, Qz'z'?0.360 MHz, and Qx'x'y'x'?0.349 MHz. These values are in agreement with the results of the nuclear modulation effect [J. Chem. Phys. 69, 4921 (1978)]. The values for the quadrupole constants are thought to be accurate within 10% and are the same as are found in free imidazole. It is also demonstrated that, in this instance, ENDOR and the nuclear modulation effect are complementary in that they have each provided different parts of the same hyperfine spectrum.

  12. Structure determination of picolinato copper(II)-amine complexes

    NASA Astrophysics Data System (ADS)

    Mautner, Franz A.; Massoud, Salah S.

    2007-12-01

    Two series of Cu(II)-picolinato complexes of 1:1 and 3:2 Cu(II)-amine/picolinate namely [Cu(L 1)(pic)]ClO 4 and [Cu 3(L 2) 3(pic) 2(H 2O)](ClO 4) 4· xH 2O or [Cu 3(dpt) 3(pic) 2](ClO 4) 4, where pic = picolinate anion, L 1 = dien (diethylenetriamine), Et 2dien ( N, N-diethyldiethylenetriamine), Medpt (3,3'-diamino- N-methyldipropylamine), L 2 = pmedien ( N, N, N', N″, N″-pentamethyl-diethylenetriamine), TPA (tris(2-pyridylmethyl)amine), and dpt = dipropylenetriamine were synthesized and structurally characterized by electronic and IR spectroscopy. Single crystal X-ray diffraction analysis of the complex [Cu(dien)(pic)]ClO 4 ( 1) reveals its monomeric nature whereas for [Cu 3(pmedien) 3(pic) 2(H 2O)](ClO 4) 4·2H 2O ( 4), it was shown that the complex consists of two subunits of the mononuclear [Cu(pmedien)(pic)] + and the dinuclear [Cu 2(pmedien) 2(pic)(H 2O)] 3+ cations with the perchlorate as counter ions and lattice water molecules. In the mononuclear complexes of 1 and 4 the picolinato anions act as N, O-chelating ligands, whereas N, O, O'-picolinato bridges are observed in the dinuclear [Cu 2(pmedien) 2(pic)(H 2O)] 3+ cations of 4. The aqueous visible spectra of the complexes 1- 6 are consistent with five-coordinate Cu(II) species where distorted square pyramidal geometry (SP) was assigned for complexes 2- 5, trigonal bipyramidal geometry (TBP) for 6 and an intermediate geometry between SP and TBP for 1.

  13. Application of copper sulfate pentahydrate as an ammonia removal reagent for the determination of trace impurities in ammonia by gas chromatography.

    PubMed

    Aomura, Yoko; Kobayashi, Yoshihiko; Miyazawa, Yuzuru; Shimizu, Hideharu

    2010-03-12

    Rapid analysis of trace permanent gas impurities in high purity ammonia gas for the microelectronics industry is described, using a gas chromatograph equipped with a phtoionization detector. Our system incorporates a reactive precolumn in combination with the analytical column to remove the ammonia matrix peak that otherwise would complicate the measurements due to baseline fluctuations and loss of analytes. The performance of 21 precolumn candidate materials was evaluated. Copper sulfate pentahydrate (CuSO(4).5H(2)O) was shown to selectively react with ammonia at room temperature and atmospheric column pressures, without affecting the hydrogen, oxygen, nitrogen, methane or carbon monoxide peak areas. To prevent loss of trace carbon dioxide, an additional boron trioxide reactant layer was inserted above the copper sulfate pentahydrate bed in the reactive precolumn. Using the combined materials, calibration curves for carbon dioxide proved to be equivalent in both ammonia and helium matrix gases. These curves were equivalent in both matrix gases. The quantitative performance of the system was also evaluated. Peak repeatabilities, based on eight injections, were in the range of 4.1-8.2% relative standard deviation; and detection limits were 6.9 ppb for H(2), 1.8 ppb for O(2), 1.6 ppb for N(2), 6.4 ppb for CH(4), 13 ppb for CO, and 5.4 ppb for CO(2). PMID:20132934

  14. Sodium dodecyl sulfate monomers induce XAO peptide polyproline II to α-helix transition.

    PubMed

    Hong, Zhenmin; Damodaran, Krishnan; Asher, Sanford A

    2014-09-11

    XAO peptide (Ac-X2A7O2-NH2; X: diaminobutyric acid side chain, -CH2CH2NH3(+); O: ornithine side chain, -CH2CH2CH2NH3(+)) in aqueous solution shows a predominantly polyproline II (PPII) conformation without any detectable α-helix-like conformations. Here we demonstrate by using circular dichroism (CD), ultraviolet resonance Raman (UVRR) and nuclear magnetic resonance (NMR) spectroscopy that sodium dodecyl sulfate (SDS) monomers bind to XAO and induce formation of α-helix-like conformations. The stoichiometry and the association constants of SDS and XAO were determined from the XAO-SDS diffusion coefficients measured by pulsed field gradient NMR. We developed a model for the formation of XAO-SDS aggregate α-helix-like conformations. Using UVRR spectroscopy, we calculated the Ramachandran ψ angle distributions of aggregated XAO peptides. We resolved α-, π- and 3(10)-helical conformations and a turn conformation. XAO nucleates SDS aggregation at SDS concentrations below the SDS critical micelle concentration. The XAO4-SDS16 aggregates have four SDS molecules bound to each XAO to neutralize the four side chain cationic charges. We propose that the SDS alkyl chains partition into a hydrophobic core to minimize the hydrophobic area exposed to water. Neutralization of the flanking XAO charges enables α-helix formation. Four XAO-SDS4 aggregates form a complex with an SDS alkyl chain-dominated hydrophobic core and a more hydrophilic shell where one face of the α-helix peptide contacts the water environment. PMID:25121643

  15. Sodium Dodecyl Sulfate Monomers Induce XAO Peptide Polyproline II to α-Helix Transition

    PubMed Central

    2015-01-01

    XAO peptide (Ac–X2A7O2–NH2; X: diaminobutyric acid side chain, −CH2CH2NH3+; O: ornithine side chain, −CH2CH2CH2NH3+) in aqueous solution shows a predominantly polyproline II (PPII) conformation without any detectable α-helix-like conformations. Here we demonstrate by using circular dichroism (CD), ultraviolet resonance Raman (UVRR) and nuclear magnetic resonance (NMR) spectroscopy that sodium dodecyl sulfate (SDS) monomers bind to XAO and induce formation of α-helix-like conformations. The stoichiometry and the association constants of SDS and XAO were determined from the XAO–SDS diffusion coefficients measured by pulsed field gradient NMR. We developed a model for the formation of XAO–SDS aggregate α-helix-like conformations. Using UVRR spectroscopy, we calculated the Ramachandran ψ angle distributions of aggregated XAO peptides. We resolved α-, π- and 310- helical conformations and a turn conformation. XAO nucleates SDS aggregation at SDS concentrations below the SDS critical micelle concentration. The XAO4–SDS16 aggregates have four SDS molecules bound to each XAO to neutralize the four side chain cationic charges. We propose that the SDS alkyl chains partition into a hydrophobic core to minimize the hydrophobic area exposed to water. Neutralization of the flanking XAO charges enables α-helix formation. Four XAO–SDS4 aggregates form a complex with an SDS alkyl chain-dominated hydrophobic core and a more hydrophilic shell where one face of the α-helix peptide contacts the water environment. PMID:25121643

  16. Synthesis of four binuclear copper(II) complexes: Structure, anticancer properties and anticancer mechanism.

    PubMed

    Qi, Jinxu; Liang, Shichu; Gou, Yi; Zhang, Zhenlei; Zhou, Zuping; Yang, Feng; Liang, Hong

    2015-01-01

    Copper (Cu) compounds are a promising candidate for next generation metal anticancer drugs and have been extensively studied. Therefore, four binuclear copper(II) compounds derived from Schiff base thiosemicarbazones (L1-L4), namely [CuCl(L1)]2 (C1), [CuNO3(L2)]2 (C2), [Cu(NCS) (L3)]2 (C3) and [Cu(CH3COO) (L4)]2 (C4) were synthesized and characterized. Four of these compounds showed very high cytotoxicity to cancer cell lines in vitro. These Cu(II) compounds strongly promoted the apoptosis of BEL-7404 cells. The formation of reactive oxygen species (ROS), change in mitochondrial membrane potential and western blot analysis revealed that Cu compounds could induce cancer cell apoptosis through the intrinsic ROS-mediated mitochondrial pathway accompanied by the regulation of Bcl-2 family proteins. PMID:25899339

  17. Processing of O.F.E. copper beam chambers for PEP-II high energy ring

    SciTech Connect

    Hoyt, E.; Hoyt, M.; Kirby, R.; Perkins, C.; Wright, D.; Farvid, A.

    1995-08-01

    Using laboratory scale and full size PEP-II vacuum chambers, chemical cleaning, glow discharge and thermal process effects were evaluated using surface analysis by x-ray photoelectron spectroscopy (XPS). These processes were optimized to reduce surface carbon and thereby minimize photodesorption gas loads. The relation of surface carbon to ion dose was investigated and compared for pure argon, 5% oxygen in argon, and pure hydrogen plasmas. Argon incorporation was noted only when the copper was oxidized in the mixed gas. Surfaces, stable in ambient atmosphere, were obtained having surface carbon values less than 10%. These optimized recipes will be used in processing copper vacuum chambers for the PEP-II B-Factory.

  18. Potential complex of rhodamine B and copper (II) for dye sensitizer on solar cell

    NASA Astrophysics Data System (ADS)

    Setyawati, Harsasi; Purwaningsih, Aning; Darmokoesoemo, Handoko; Hamami, Rochman, Faidur; Permana, Ahmadi Jaya

    2016-03-01

    A complex from copper(II) and rhodamine B as ligand was synthesized, characterized and applied as potential dye sensitizer on solar cell. A complex was synthesized from the reaction of copper(II) salts and rhodamine B with mole ratio 1:3. A complex showing Metal Ligand Charge Transfer (MLCT) phenomenon at 260 nm. Metal-ligand bonding through carbonyl (CO) groups at 617.22 cm-1 and methoxy (CH3O) groups at 339.47 cm-1. Electrical conductivity analysis confirms that the complex was ionic compound. The complex was applied as potential dye sensitizer with open circuit voltage 0.48775 V, short circuit current 0.01025 mA/cm2 and efficiency 0.0039 %.

  19. Antifouling bastadin congeners target mussel phenoloxidase and complex copper(II) ions.

    PubMed

    Bayer, Mirko; Hellio, Claire; Maréchal, Jean-Philippe; Frank, Walter; Lin, Wenhan; Weber, Horst; Proksch, Peter

    2011-12-01

    Synthetically prepared congeners of sponge-derived bastadin derivatives such as 5,5'-dibromohemibastadin-1 (DBHB) that suppress the settling of barnacle larvae were identified in this study as strong inhibitors of blue mussel phenoloxidase that is involved in the firm attachment of mussels to a given substrate. The IC₅₀ value of DBHB as the most active enzyme inhibitor encountered in this study amounts to 0.84 μM. Inhibition of phenoloxidase by DBHB is likely due to complexation of copper(II) ions from the catalytic centre of the enzyme by the α-oxo-oxime moiety of the compound as shown here for the first time by structure activity studies and by X-ray structure determination of a copper(II) complex of DBHB. PMID:21547349

  20. A new dioxime ligand and its trinuclear copper(II) complex: Synthesis, characterization and optical properties

    NASA Astrophysics Data System (ADS)

    Karipcin, Fatma; Dede, Bülent; Caglar, Yasemin; Hür, Deniz; Ilican, Saliha; Caglar, Mujdat; Şahin, Yücel

    2007-04-01

    The synthesis and characterization of a new dioxime ligand, (H2L), (III) and its trinuclear copper(II) complex, [Cu3L2(H2O)2](ClO4)2, (V) is described. IR spectra show that the ligand acts in a tetradentate manner and coordinates N4 donor groups of H2L to copper(II) ion. The structure of the metal chelate is clarified with the help of elemental analysis, magnetic moment, conductometric and spectroscopic measurements. The optical absorption studies reveal that the transition is direct with band gap energy values are calculated. The optical constants such as refractive index and dielectric constant of the compound were determined. The refractive index dispersion curves of the films obey the single-oscillator model and oscillator parameters. Optical dispersion parameters E0 and Ed developed by Wemple-DiDomenico were calculated.

  1. The computed distribution of copper(II) and zinc(II) ions among seventeen amino acids present in human blood plasma

    PubMed Central

    Hallman, P. S.; Perrin, D. D.; Watt, Ann E.

    1971-01-01

    The equilibrium distribution of copper(II) and zinc(II) ions among a mixture of 17 amino acids has been computed from stability-constant and blood-plasma-composition data. At pH7.4, 98% of the copper(II) in the simulated plasma solution is co-ordinated to histidine and cystine, predominantly as the mixed-ligand complexes [Cu·His·Cystine]− and [Cu·H·His·Cystine]. Approximately half of the zinc(II) is co-ordinated to cysteine and histidine, but appreciable complex-formation occurs with most of the other amino acids. Stability constants are given for copper(II) and zinc(II) amino acid complexes, including some mixed-ligand species, at 37°C and I=0.15m. PMID:5119792

  2. The effect of chain length on the thermal stability of 2-alkylimidazoles on copper and 2-alkylimidazolato copper(II) complexes

    NASA Astrophysics Data System (ADS)

    Yoshida, Shuji; Ishida, Hatsuo

    1985-02-01

    The molecular structure and thermal stability of imidazole, 2-methylimidazole, 2-ethylimidazole, 2-propylimidazole, 2-undecylimidazole, and 2-heptadecylimidazole on copper are studied using FT-IR reflection—absorption spectroscopy. As model compounds of these complexes formed on copper, corresponding imidazole copper(II) complexes are synthesized in solution. The complexes appear to be the semicrystalline or crystalline structure. The series of imidazoles employed on copper indicates the same oxidation and degradation process at high temperatures even though imidazole, 2-methylimidazole and 2-ethylimidazole possess better thermal stability below 150°C. The synthesized complexes indicate poor thermal stability as the chain length of the alkyl substituent increases and the enhanced oxidation by the addition of copper powder. The difference in oxidation between imidazoles on copper and synthesized imidazole complexes are due to the presence of copper ion migrating from the copper substrate to catalyze oxidation and different morphological structure. The difference in oxidation among the imidazole derivatives depend on the melting points and the fraction of the imidazole ring which occupies the molecule.

  3. Infrared spectroscopic study of copper(II) complexes with N-substituted tetrazoles1

    NASA Astrophysics Data System (ADS)

    Degtyarik, M. M.; Gaponik, P. N.; Naumenko, V. N.; Lesnikovich, A. I.; Nikanovich, M. V.

    The i.r. spectra (4000-200 cm -1) of N-alkyltetrazoles and their complexes with copper(II) salts CuCl 2 and Cu(NCS) 2 have been measured. Spectral criteria in the middle and far regions for the identification of tetrazole isomers and their complexes are proposed. The site of the coordination in a tetrazole molecule and supposed structure of complexes are being discussed.

  4. Complexes of sulfur-containing ligands. I. Factors influencing complex formation between D-penicillamine and copper (II) ion.

    PubMed

    Gergely, A; Sóvágó, I

    1978-07-01

    Complex formation and redox reactions between copper (II) ion and D-penicillamine were studied in detail as functions of the metal/-ligand ratio and the concentration of halide ions. It was established that a copper (I)- D-penicillamine polymeric complex of amphoteric character is formed when excess D-penicillamine is present. When the D-penicillamine/copper (II) ratio = 1.45 in the starting reaction mixture, a mixed valence complex with an intense red-violet color is formed. The formation of this compound, which contains 44% copper (II) ion, is greatly influenced by the experimental conditions, primarily by the concentration of halide ions. The main chemical and physical characteristics of the mixed valence complex were determined via magnetic and spectroscopic measurements. It was further established that a very intense blue complex is formed when the D-penicillamine/copper (II) ratio = 2 and halide ions are present. On the basis of the nature of the products formed under various conditions it was concluded that the copper (II)-D-penicillamine system may serve as a good model for studying the binding sites of copper-containing proteins. PMID:210846

  5. An unusual 3D interdigitated architecture assembled from Keggin polyoxometalates and dinuclear copper(II) complexes

    SciTech Connect

    Pang, Haijun; Yang, Ming; Kang, Lu; Ma, Huiyuan; Liu, Bo; Li, Shaobin; Liu, Heng

    2013-02-15

    A novel organic-inorganic hybrid compound, [Cu{sub 2}(bipy){sub 3}({mu}{sub 1}-H{sub 2}O){sub 2}({mu}{sub 2}-H{sub 2}O)({mu}{sub 2}-OH)(H{sub 2}BW{sub 12}O{sub 40})]{center_dot}4 H{sub 2}O (1) (bipy=4,4 Prime -bipy), has been synthesized in hydrothermal condition and characterized by elemental analysis, IR spectrum, TG analysis and single-crystal X-ray diffraction. Compound 1 possesses poly-pendant layered motifs composed of 12-tungstoborates and dinuclear copper(II) complexes, in which the mono-coordinated bipy molecules are orderly appended to both sides of the layer, respectively. Adjacent layers mutually engage in a zipper-like pattern to result in a novel 3D interdigitated architecture. The variable-temperature magnetic susceptibility of 1 showed that there existed weak antiferromagnetic interaction in 1. Toward the reduction of hydrogen peroxide, 1 has good electrocatalytic activity and remarkable stability. - A new compound has been obtained, which represents the first interdigitated architecture assembled by POMs and dinuclear copper(II) complexes. Highlights: Black-Right-Pointing-Pointer The first example of interdigitated architecture assembled by POMs and dinuclear copper(II) complexes is observed. Black-Right-Pointing-Pointer A zipper-like pattern is observed in the structure. Black-Right-Pointing-Pointer The IR, TG, XRPD, magnetism and electrochemical property of the title compound were studied.

  6. STUDIES ON BIOSORPTION OF ZINC(II) AND COPPER(II) ON DESULFOVIBRIO DESULFURICANS

    EPA Science Inventory

    The objectives of thes studies are to determine the equilibrium concentration and kinetics of metal sorption on sulfate-reducing bacteria (SRB) isolates. Adsorption establishes the net reversible cellular metal uptake and is related to SRB metal toxicity and the effects of enviro...

  7. Kinetic and Spectroscopic Studies of Aerobic Copper(II)-Catalyzed Methoxylation of Arylboronic Esters and Insights into Aryl Transmetalation to Copper(II).

    PubMed

    King, Amanda E; Ryland, Bradford L; Brunold, Thomas C; Stahl, Shannon S

    2012-11-26

    We previously reported a preliminary mechanistic study of aerobic Cu(OAc)(2)-catalyzed methoxylation of 4-tolylboronic ester (King, et al. J. Am. Chem. Soc., 2009, 131, 5044-5045), which revealed that aryl transmetalation from the boronic ester to Cu(II) is the turnover-limiting step. In the present study, more-thorough kinetic and spectroscopic studies provide additional insights into transmetalation pathway and the identity of the Cu(II) catalyst resting state(s). EPR spectroscopic studies show that at least two copper(II) species are present under catalytic conditions and their relative populations vary as a function of reaction time and acidity of the arylboronic ester, and are influenced by addition of acetic acid or acetate to the reaction mixture. Analysis of kinetic data and (11)B NMR and EPR spectra under diverse reaction conditions suggests that aryl transmetalation occurs from a tetracoordinate, anionic boronate to a cationic Cu(II) species, mediated by a methoxide-bridge. PMID:23204631

  8. Nucleophilic reactivity of a copper(II)-superoxide complex.

    PubMed

    Pirovano, Paolo; Magherusan, Adriana M; McGlynn, Ciara; Ure, Andrew; Lynes, Amy; McDonald, Aidan R

    2014-06-01

    Metal-bound superoxide intermediates are often implicated as electrophilic oxidants in dioxygen-activating metalloenzymes. In the nonheme iron α-ketoglutarate dependent oxygenases and pterin-dependent hydroxylases, however, Fe(III)-superoxide intermediates are postulated to react by nucleophilic attack on electrophilic carbon atoms. By reacting a Cu(II)-superoxide complex (1) with acyl chloride substrates, we have found that a metal-superoxide complex can be a very reactive nucleophile. Furthermore, 1 was found to be an efficient nucleophilic deformylating reagent, capable of Baeyer-Villiger oxidation of a number of aldehyde substrates. The observed nucleophilic chemistry represents a new domain for metal-superoxide reactivity. Our observations provide support for the postulated role of metal-superoxide intermediates in nonheme iron α-ketoglutarate dependent and pterin-dependent enzymes. PMID:24753290

  9. EPR, mass, IR, electronic, and magnetic studies on copper(II) complexes of semicarbazones and thiosemicarbazones

    NASA Astrophysics Data System (ADS)

    Chandra, Sulekh; Gupta, Lokesh Kumar

    2005-01-01

    Copper(II) complexes having the general composition Cu(L) 2X 2 [where L = isopropyl methyl ketone semicarbazone (LLA), isopropyl methyl ketone thiosemicarbazone (LLB), 4-aminoacetophenone semicarbazone (LLC), and 4-aminoacetophenone thiosemicarbazone (LLD) and X = Cl -, 1/2SO 42-] have been synthesized. All the Cu(II) complexes reported here have been characterized by elemental analyses, molar conductance, magnetic moment susceptibility, EI mass, 1H NMR, IR, EPR, and electronic spectral studies. All the complexes were found to have magnetic moments corresponding to one unpaired electrons. The possible geometries of the complexes were assigned on the basis of EPR, electronic, and infrared spectral studies.

  10. A new probe of solvent accessibility of bound photosensitizers. 1. Ruthenium(II) and osmium(II) photosensitizers in sodium lauryl sulfate micelles

    SciTech Connect

    Hauenstein, B.L. Jr.; Dressick, W.J.; Buell, S.L.; Demas, J.N.; DeGraff, B.A.

    1983-06-29

    A new method of measuring solvent accessibility of photosensitizers bound to organized media is presented. In particular, the solvent accessibility of a series of ruthenium(II) and osmium(II) photosensitizers bound to sodium lauryl sulfate micelles has been determined. The method takes advantage of the large solvent deuterium effect on the excited-state lifetimes of these complexes. The solvent accessibility of the bound complexes correlates with the hydrophobicity of the ligands. The potential application of this method to a variety of other systems is mentioned.