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Sample records for corn streak virus

  1. Ipomoviruses: Squash vein yellowing virus, Cucumber vein yellowing virus, Cassava brown streak virus, and Ugandan cassava brown streak virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ipomoviruses including Squash vein yellowing virus, Cucumber vein yellowing virus and Cassava brown streak virus are currently causing significant economic impact on crop production in several regions of the world. Only recently have results of detailed characterization of their whitefly transmissi...

  2. Comparative analysis of Panicum streak virus and Maize streak virus diversity, recombination patterns and phylogeography

    PubMed Central

    2009-01-01

    Background Panicum streak virus (PanSV; Family Geminiviridae; Genus Mastrevirus) is a close relative of Maize streak virus (MSV), the most serious viral threat to maize production in Africa. PanSV and MSV have the same leafhopper vector species, largely overlapping natural host ranges and similar geographical distributions across Africa and its associated Indian Ocean Islands. Unlike MSV, however, PanSV has no known economic relevance. Results Here we report on 16 new PanSV full genome sequences sampled throughout Africa and use these together with others in public databases to reveal that PanSV and MSV populations in general share very similar patterns of genetic exchange and geographically structured diversity. A potentially important difference between the species, however, is that the movement of MSV strains throughout Africa is apparently less constrained than that of PanSV strains. Interestingly the MSV-A strain which causes maize streak disease is apparently the most mobile of all the PanSV and MSV strains investigated. Conclusion We therefore hypothesize that the generally increased mobility of MSV relative to other closely related species such as PanSV, may have been an important evolutionary step in the eventual emergence of MSV-A as a serious agricultural pathogen. The GenBank accession numbers for the sequences reported in this paper are GQ415386-GQ415401 PMID:19903330

  3. Inducible Resistance to Maize Streak Virus

    PubMed Central

    Shepherd, Dionne N.; Dugdale, Benjamin; Martin, Darren P.; Varsani, Arvind; Lakay, Francisco M.; Bezuidenhout, Marion E.; Monjane, Adérito L.; Thomson, Jennifer A.; Dale, James; Rybicki, Edward P.

    2014-01-01

    Maize streak virus (MSV), which causes maize streak disease (MSD), is the major viral pathogenic constraint on maize production in Africa. Type member of the Mastrevirus genus in the family Geminiviridae, MSV has a 2.7 kb, single-stranded circular DNA genome encoding a coat protein, movement protein, and the two replication-associated proteins Rep and RepA. While we have previously developed MSV-resistant transgenic maize lines constitutively expressing “dominant negative mutant” versions of the MSV Rep, the only transgenes we could use were those that caused no developmental defects during the regeneration of plants in tissue culture. A better transgene expression system would be an inducible one, where resistance-conferring transgenes are expressed only in MSV-infected cells. However, most known inducible transgene expression systems are hampered by background or “leaky” expression in the absence of the inducer. Here we describe an adaptation of the recently developed INPACT system to express MSV-derived resistance genes in cell culture. Split gene cassette constructs (SGCs) were developed containing three different transgenes in combination with three different promoter sequences. In each SGC, the transgene was split such that it would be translatable only in the presence of an infecting MSV’s replication associated protein. We used a quantitative real-time PCR assay to show that one of these SGCs (pSPLITrepIII-Rb-Ubi) inducibly inhibits MSV replication as efficiently as does a constitutively expressed transgene that has previously proven effective in protecting transgenic maize from MSV. In addition, in our cell-culture based assay pSPLITrepIII-Rb-Ubi inhibited replication of diverse MSV strains, and even, albeit to a lesser extent, of a different mastrevirus species. The application of this new technology to MSV resistance in maize could allow a better, more acceptable product. PMID:25166274

  4. Wheat streak mosaic virus-Structural parameters for a Potyvirus

    SciTech Connect

    Parker, Lauren; Kendall, Amy; Berger, P.H.; Shiel, P.J.; Stubbs, Gerald . E-mail: gerald.stubbs@vanderbilt.edu

    2005-09-15

    Wheat streak mosaic virus is a Tritimovirus, a member of the Potyviridae family, which includes the very large Potyvirus genus. We have examined wheat streak mosaic virus by electron microscopy and fiber diffraction from partially oriented sols, and analyzed the results to estimate the symmetry and structural parameters of the viral helix. The virions have an apparent radius of 63 {+-} 5 A. The viral helix has a pitch of 33.4 A {+-} 0.6 A. There appear to be 6.9 subunits per turn of the helix, although we cannot completely eliminate values of 5.9 or 7.9 for this parameter.

  5. Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants

    PubMed Central

    Lee, Siwon; Kim, Jin-Ho; Choi, Ji-Young; Jang, Won-Cheoul

    2015-01-01

    We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections. PMID:26674930

  6. Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants.

    PubMed

    Lee, Siwon; Kim, Jin-Ho; Choi, Ji-Young; Jang, Won-Cheoul

    2015-12-01

    We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections. PMID:26674930

  7. Characterization of Brown Streak Virus-Resistant Cassava.

    PubMed

    Anjanappa, Ravi B; Mehta, Devang; Maruthi, M N; Kanju, Edward; Gruissem, Wilhelm; Vanderschuren, Hervé

    2016-07-01

    Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies, as the disease is progressing westwards to new geographical areas. A stringent infection method based on top cleft-grafting combined with precise virus titer quantitation was utilized to screen 14 cassava cultivars and elite breeding lines. When inoculated with mixed infections of Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the scions of elite breeding lines KBH 2006/18 and KBH 2006/26 remained symptom-free during a 16-week period of virus graft inoculation, while susceptible varieties displayed typical CBSD infection symptoms at 4 weeks after grafting. The identified CBSD resistance was stable under the coinoculation of CBSV and UCBSV with cassava geminiviruses. Double-grafting experiments revealed that transmission of CBSV and UCBSV to CBSD-susceptible top scions was delayed when using intermediate scions of elite breeding lines KBH 2006/18 and KBH 2006/26. Nonetheless, comparison of virus systemic movement using scions from KBH2006/18 and a transgenic CBSD resistant 60444 line (60444-Hp9 line) showed that both CBSV and UCBSV move at undetectable levels through the stems. Further, protoplast-based assays of virus titers showed that the replication of CBSV is inhibited in the resistant line KBH2006/18, suggesting that the identified CBSD resistance is at least partially based on inhibition of virus replication. Our molecular characterization of CBSD resistance in cassava offers a robust virus-host system to further investigate the molecular determinants of CBSD resistance. PMID:27070326

  8. Development of a multiplexed PCR detection method for Barley and Cereal Yellow Dwarf Viruses, Wheat Spindle Streak Virus, Wheat Streak Mosaic Virus and Soil-Borne Wheat Mosaic Virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Barley and Cereal Yellow Dwarf Viruses (B/CYDVs), Wheat Spindle Streak Mosaic (WSSMV), Soil-Borne Wheat Mosaic (SBWMV) Mosaic Virus and Wheat Streak Mosaic Virus (WSMV) constitute the most economically important group of wheat viruses. In this paper, a multiplex reverse transcription polymerase chai...

  9. Multiplex Real Time PCR For Detection of Wheat Streak Mosaic Virus and Triticum Mosaic Virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TRIMV) are widespread throughout the southwestern Great Plains states. Using conventional diagnostics such as Enzyme-Linked Immunosorbent Assays (ELISA), these two viruses are commonly found together in infected wheat samples. Methods for m...

  10. Complete genome sequence of a novel badnavirus, banana streak IM virus.

    PubMed

    Geering, Andrew D W; Parry, Judith N; Thomas, John E

    2011-04-01

    In 1999, banana streak disease outbreaks occurred at two locations in Australia in new banana hybrids that were being screened for fusarium wilt resistance. Two different badnaviruses, banana streak GF virus and a newly discovered virus called banana streak IM virus (BSIMV), were detected in these plants. The complete nucleotide sequence of the BSIMV genome was determined and comprised 7768 nt. Three open reading frames were detected, the first beginning with a non-conventional start codon (CUG). A 55-nt repetition in the putative pregenomic RNA promoter was also identified. Phylogenetic analysis suggests that BSIMV is most closely related to banana streak VN virus. PMID:21347843

  11. Cloning and sequence analysis of banana streak virus DNA.

    PubMed

    Harper, G; Hull, R

    1998-01-01

    Banana streak virus (BSV), a member of the Badnavirus group of plant viruses, causes severe problems in banana cultivation, reducing fruit yield and restricting plant breeding and the movement of germplasm. Current detection methods are relatively insensitive. In order to develop a PCR-based diagnostic method that is both reliable and sensitive, the genome of a Nigerian isolate of BSV has been sequenced and shown to comprise 7389 bp and to be organized in a manner characteristic of badnaviruses. Comparison of this sequence with those of other badnaviruses showed that BSV is a distinct virus. PCR with primers based on sequence data indicated that BSV sequences are present in the banana genome. PMID:9926402

  12. Analyses of Twelve New Whole Genome Sequences of Cassava Brown Streak Viruses and Ugandan Cassava Brown Streak Viruses from East Africa: Diversity, Supercomputing and Evidence for Further Speciation.

    PubMed

    Ndunguru, Joseph; Sseruwagi, Peter; Tairo, Fred; Stomeo, Francesca; Maina, Solomon; Djikeng, Appolinaire; Djinkeng, Appolinaire; Kehoe, Monica; Boykin, Laura M

    2015-01-01

    Cassava brown streak disease is caused by two devastating viruses, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) which are frequently found infecting cassava, one of sub-Saharan Africa's most important staple food crops. Each year these viruses cause losses of up to $100 million USD and can leave entire families without their primary food source, for an entire year. Twelve new whole genomes, including seven of CBSV and five of UCBSV were uncovered in this research, doubling the genomic sequences available in the public domain for these viruses. These new sequences disprove the assumption that the viruses are limited by agro-ecological zones, show that current diagnostic primers are insufficient to provide confident diagnosis of these viruses and give rise to the possibility that there may be as many as four distinct species of virus. Utilizing NGS sequencing technologies and proper phylogenetic practices will rapidly increase the solution to sustainable cassava production. PMID:26439260

  13. Analyses of Twelve New Whole Genome Sequences of Cassava Brown Streak Viruses and Ugandan Cassava Brown Streak Viruses from East Africa: Diversity, Supercomputing and Evidence for Further Speciation

    PubMed Central

    Ndunguru, Joseph; Sseruwagi, Peter; Tairo, Fred; Stomeo, Francesca; Maina, Solomon; Djinkeng, Appolinaire; Kehoe, Monica; Boykin, Laura M.

    2015-01-01

    Cassava brown streak disease is caused by two devastating viruses, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) which are frequently found infecting cassava, one of sub-Saharan Africa’s most important staple food crops. Each year these viruses cause losses of up to $100 million USD and can leave entire families without their primary food source, for an entire year. Twelve new whole genomes, including seven of CBSV and five of UCBSV were uncovered in this research, doubling the genomic sequences available in the public domain for these viruses. These new sequences disprove the assumption that the viruses are limited by agro-ecological zones, show that current diagnostic primers are insufficient to provide confident diagnosis of these viruses and give rise to the possibility that there may be as many as four distinct species of virus. Utilizing NGS sequencing technologies and proper phylogenetic practices will rapidly increase the solution to sustainable cassava production. PMID:26439260

  14. Quantification of yield loss caused by Triticum mosaic virus and Wheat streak mosaic virus in winter wheat under field conditions

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Triticum mosaic virus (TriMV) and Wheat streak mosaic virus (WSMV) infect winter wheat in the Great Plains region of the United States. The two viruses are transmitted by wheat curl mites, which also transmit High Plains virus. In a field study conducted in 2011 and 2012, winter wheat cultivars Mi...

  15. Resistance to wheat streak mosaic virus and Triticum mosaic virus in wheat lines carrying Wsm1 and Wsm3

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are important viruses of wheat (Triticum aestivum L.) in the Great Plains of United States. In addition to agronomic practices to prevent damage from these viruses, temperature sensitive resistance genes Wsm1, Wsm2 and Wsm3, have bee...

  16. Role of wheat streak mosaic virus-encoded proteins in disease development

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Eriophyid mite-transmitted wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are the type species of Tritimovirus and Poacevirus genera, respectively, in the family Potyviridae. TriMV and WSMV exhibit differential symptom phenotypes on wheat: TriMV elicits mild mosaic and mottling ...

  17. Nuclear import of Maize fine streak virus proteins in Drosophila S2 cells

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize fine streak virus (MFSV) is a member of the genus Nucleorhabdovirus, family Rhabdoviridae and is transmitted by the leafhopper Graminella nigrifons. The virus replicates in both its plant host and in its insect vector. Nucleorhabdoviruses replicate in the nucleus and assemble at the inner nu...

  18. Efficient inoculation of rice black-streaked dwarf virus to maize using Laodelphax striatellus Fallen

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize rough dwarf disease (MRDD) caused by Rice black-streaked dwarf virus (RBSDV) is the most important viral disease of maize in China. Although deploying disease resistant hybrids would be the most effective way to control the disease, development of resistant hybrids has been limited by virus t...

  19. Economic impact of wheat streak mosaic virus in the Texas High Plains

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV), vectored by the wheat curl mite Aceria tosichella Keifer, is a major limiting factor in wheat production in the Texas Panhandle. It is the most frequently encountered virus in the region, affecting both shoot and root biomass, and consequently it can drastically red...

  20. Plant host range and leafhopper transmission of Maize fine streak virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize fine streak virus (MFSV), an emerging rhabdovirus species in the genus Nucleorhabdovirus, is persistently transmitted by the black-faced leafhopper, Graminella nigrifrons (Forbes). MFSV was transmitted to maize, wheat, oats, rye, barley, foxtail, annual ryegrass and quackgrass by G. nigrifron...

  1. Chromatin Structure of Wheat Breeding Lines Resistant to Wheat Streak Mosaic Virus.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat Streak Mosaic Virus (WSMV) is an important disease limiting wheat production, however no WSMV resistance effective above 18°C is present within the primary genetic pool of wheat (Triticum aestivum L.). In contrast, the wild relative Thinopyrum intermedium (2n=6x=42) shows good resistance to WS...

  2. Physiological responses of hard red winter wheat to infection by wheat streak mosaic virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV) causes significant yield loss in hard red winter wheat in the U.S. Southern High Plains. Despite the prevalence of this pathogen, little is known about the physiological response of wheat to WSMV infection. A 2-year study was initiated to (i) investigate the effect o...

  3. Complete genome sequence of an emerging genotype of tobacco streak virus in the U.S.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We report the complete genome sequence of an emerging genotype of Tobacco streak virus (TSV) infecting zucchini squash in Florida (TSV_FL13-07), through deep sequencing of sRNAs and validation by Sanger sequencing. TSV_FL13-07 only shares less than 90% sequence identity in three genomic ribonucleic...

  4. Diverse soybean accessions identified with temperature-sensitive resistance to Tobacco streak virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tobacco streak virus (TSV) is a pathogen that has been reported in soybean in Brazil and the United States, for which resistance has only been reported in the “hay-type” soybean cultivar Tanner. To find additional resistant soybean genotypes, over 1000 soybean accessions from the USDA Soybean Germpl...

  5. Incidence of Wheat streak mosaic virus, Triticum mosaic virus, and Wheat mosaic virus in wheat curl mites recovered from maturing winter wheat spikes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat curl mites (WCM; Aceria tosichella) transmit Wheat streak mosaic virus (WSMV), Triticum mosaic virus (TriMV), and Wheat mosaic virus (WMoV) to wheat (Triticum aestivum L.) in the Great Plains region of the United States. These viruses can be detected in single, double, or triple combinations i...

  6. A compensating wheat-Thinopyrum intermedium Robertsonian translocation conferring resistance to wheat streak mosaic virus and Triticum mosaic virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV), is a potentially devastating disease of common wheat in the Great Plains of North America. So far, two genes conferring resistance to WSMV have been named and used in cultivar improvement. Here we report a new source of resistance that was derived from a wheat-Th. i...

  7. Identification of distinct functions of Wheat streak mosaic virus coat protein in virion assembly and virus movement

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV) is the type member of Tritimovirus genus of the family Potyviridae. The WSMV coat protein (CP) was subjected to point and deletion mutation analyses. WSMV mutants changing aspartic acid residues at amino acid (aa) positions 289, 290, 326, 333, and 334 to alanine elic...

  8. Complete genome sequence of an emerging genotype of tobacco streak virus in the United States.

    PubMed

    Padmanabhan, Chellappan; Gao, Shan; Li, Rugang; Zhang, Shouan; Fei, Zhangjun; Ling, Kai-Shu

    2014-01-01

    We report here the complete genome sequence of an emerging genotype of tobacco streak virus (TSV) infecting zucchini squash in Florida (TSV_FL13-07), obtained using deep sequencing of short RNAs (sRNAs) and validation by Sanger sequencing. TSV_FL13-07 shares only <90% sequence identity in all three genomic RNAs to several known U.S. isolates. PMID:25377714

  9. Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time.

    PubMed

    Hajano, Jamal-U-Ddin; Wang, Biao; Ren, Yingdang; Lu, Chuantao; Wang, Xifeng

    2015-10-01

    Southern rice black streaked dwarf virus (SRBSDV) and rice black streaked dwarf virus (RBSDV) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. In this study, we quantitatively compared the spatial distribution of SRBSDV and RBSDV contents over time in their vector and nonvector insects using real time-PCR. Genome equivalent copies (GEC) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period (AAP) on infected plants. Results revealed 293.2±21.6 to 404.1±46.4 SRBSDV GEC/ng total RNA in whole body of white-backed planthopper (WBPH, Sogatella furcifera) at day 0 and 12 and 513.5±88.4 to 816.8±110.7 RBSDV GEC/ng total RNA in the whole body of small brown planthopper (SBPH, Laodelphax striatellus) at day 0 and 14, respectively, after 3-days AAP. Highest GEC of both viruses were found in the gut of their respective vectors. Although SRBSDV was detected in the gut of SBPH, it did not spread into the hemolymph or other organs. After an 8-day latent period, the transmission efficiency of SRBSDV and RBSDV by their respective vectors was significantly positively correlated with GEC in the salivary gland (r(2)=0.7808, P=0.0036 and r(2)=0.9351, P<0.0001, respectively, at α=0.05). Together, these results confirm that accumulation of >200 SRBSDV or RBSDV GEC/ng total RNA in the gut of vector, indicated threshold for further spread and the virus content in the salivary gland was significantly correlated with transmission efficiency by their respective vectors. PMID:26116274

  10. Winter wheat cultivars with temperature sensitive resistance to wheat streak mosaic virus do not recover from early season infections

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV), Triticum mosaic virus, and Wheat mosaic virus, all vectored by the wheat curl mite Aceria tosichella Keifer, frequently cause devastating losses to winter wheat production throughout the central and western Great Plains. Resistant 'Mace' and 'RonL' are commercially ...

  11. The Symptom and Genetic Diversity of Cassava Brown Streak Viruses Infecting Cassava in East Africa

    PubMed Central

    Mohammed, I. U.; Abarshi, M. M.; Muli, B.; Hillocks, R. J.; Maruthi, M. N.

    2012-01-01

    The genetic and symptom diversity of six virus isolates causing cassava brown streak disease (CBSD) in the endemic (Kenya, Mozambique, and Tanzania) and the recently affected epidemic areas (Uganda) of eastern Africa was studied. Five cassava varieties; Albert, Colombian, Ebwanateraka, TMS60444 (all susceptible) and Kiroba (tolerant) were graft inoculated with each isolate. Based on a number of parameters including the severity of leaf and root symptoms, and the extent of virus transmission by grafting, the viruses were classified as either severe or relatively mild. These results were further confirmed by the mechanical inoculation of 13 herbaceous hosts in which the virulent isolates caused plant death in Nicotiana clevelandii and N. benthamiana whereas the milder isolates did not. Phylogenetic analysis of complete coat protein gene sequences of these isolates together with sequences obtained from 14 other field-collected samples from Kenya and Zanzibar, and reference sequences grouped them into two distinct clusters, representing the two species of cassava brown streak viruses. Put together, these results did not suggest the association of a hypervirulent form of the virus with the current CBSD epidemic in Uganda. Identification of the severe and milder isolates, however, has further implications for disease management and quarantine requirements. PMID:22454639

  12. RNAi-mediated resistance to Cassava brown streak Uganda virus in transgenic cassava.

    PubMed

    Yadav, Jitender S; Ogwok, Emmanuel; Wagaba, Henry; Patil, Basavaprabhu L; Bagewadi, Basavaraj; Alicai, Titus; Gaitan-Solis, Eliana; Taylor, Nigel J; Fauquet, Claude M

    2011-09-01

    Cassava brown streak disease (CBSD), caused by Cassava brown streak Uganda virus (CBSUV) and Cassava brown streak virus (CBSV), is of new epidemic importance to cassava (Manihot esculenta Crantz) production in East Africa, and an emerging threat to the crop in Central and West Africa. This study demonstrates that at least one of these two ipomoviruses, CBSUV, can be efficiently controlled using RNA interference (RNAi) technology in cassava. An RNAi construct targeting the near full-length coat protein (FL-CP) of CBSUV was expressed constitutively as a hairpin construct in cassava. Transgenic cassava lines expressing small interfering RNAs (siRNAs) against this sequence showed 100% resistance to CBSUV across replicated graft inoculation experiments. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed the presence of CBSUV in leaves and some tuberous roots from challenged controls, but not in the same tissues from transgenic plants. This is the first demonstration of RNAi-mediated resistance to the ipomovirus CBSUV in cassava. PMID:21726367

  13. Effects of single and double infections of winter wheat by Triticum mosaic virus and Wheat streak mosaic virus on yield determinants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat (Triticum aestivum L.) in the Great Plains region of the United States. It is transmitted by wheat curl mites (Aceria tosichella Keifer) which also transmit Wheat streak mosaic virus (WSMV) and Wheat mosaic virus. In a gree...

  14. Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR

    PubMed Central

    2013-01-01

    Background The diseases caused by Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) have been occurring epidemically in China and southeastern Asia in recent years. A sensitive, reliable and quantitative method is required to detect and distinguish for RBSDV and SRBSDV in rice and vector insects. Results We developed a sensitive and lineage-specific duplex real time RT-qPCR for detection of RBSDV and SRBSDV in a single or/and double infection in rice samples. The duplex RT-qPCR was optimized using standard samples transcribed by T7 Large Scale RNA Production System in vitro. We developed a reliable system for duplex RT-qPCR, in which its co-efficiency of RBSDV and SRBSDV, were 91.6% and 90.7%, respectively. The coefficient of determination was more than 0.990; the slope of linear equation was −3.542, and −3.567, respectively. Out of 30 samples collected in North and Central China, which were suspected to be infected with these two viruses, 10 samples were detected RBSDV positive by RT-PCR and 12 samples by RT-qPCR. No mixed infections were found. Simultaneously, out of total 60 samples collected from Southern China, which were also suspected to be infected with these two viruses, 41 samples were determined SRBSDV positive by RT-PCR and 47 samples by RT-qPCR. Also in this case no mixed infections were found. The rice genes eEF-1a and UBQ5 were selected as internal controls for quantification assay also performed as good expression stability. Conclusion The duplex RT-qPCR assay provided as a sufficiently sensitive, specific, accurate, reproducible and rapid tool for the detection and differentiation of RBSDV and SRBSDV. The RT-qPCR assay can be used in routine diagnostic of these two viruses in order to study the disease epidemiology in rice crops. PMID:23331990

  15. Comparative analysis of virus-derived small RNAs within cassava (Manihot esculenta Crantz) infected with cassava brown streak viruses.

    PubMed

    Ogwok, Emmanuel; Ilyas, Muhammad; Alicai, Titus; Rey, Marie E C; Taylor, Nigel J

    2016-04-01

    Infection of plant cells by viral pathogens triggers RNA silencing, an innate antiviral defense mechanism. In response to infection, small RNAs (sRNAs) are produced that associate with Argonaute (AGO)-containing silencing complexes which act to inactivate viral genomes by posttranscriptional gene silencing (PTGS). Deep sequencing was used to compare virus-derived small RNAs (vsRNAs) in cassava genotypes NASE 3, TME 204 and 60444 infected with the positive sense single-stranded RNA (+ssRNA) viruses cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the causal agents of cassava brown streak disease (CBSD). An abundance of 21-24nt vsRNAs was detected and mapped, covering the entire CBSV and UCBSV genomes. The 21nt vsRNAs were most predominant, followed by the 22 nt class with a slight bias toward sense compared to antisense polarity, and a bias for adenine and uracil bases present at the 5'-terminus. Distribution and frequency of vsRNAs differed between cassava genotypes and viral genomes. In susceptible genotypes TME 204 and 60444, CBSV-derived sRNAs were seen in greater abundance than UCBSV-derived sRNAs. NASE 3, known to be resistant to UCBSV, accumulated negligible UCBSV-derived sRNAs but high populations of CBSV-derived sRNAs. Transcript levels of cassava homologues of AGO2, DCL2 and DCL4, which are central to the gene-silencing complex, were found to be differentially regulated in CBSV- and UCBSV-infected plants across genotypes, suggesting these proteins play a role in antiviral defense. Irrespective of genotype or viral pathogen, maximum populations of vsRNAs mapped to the cytoplasmic inclusion, P1 and P3 protein-encoding regions. Our results indicate disparity between CBSV and UCBSV host-virus interaction mechanisms, and provide insight into the role of virus-induced gene silencing as a mechanism of resistance to CBSD. PMID:26811902

  16. Factors Influencing the Production of MFSV Full-Length Clone: Maize Fine Streak Virus Proteins in Drosophila S2 Cells

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize fine streak virus (MFSV) is negative-sense RNA virus member of the genus Nucleorhabdovirus. Our goal is to determine whether Drosophila S2 cells can support the production of a full-length clone of MFSV. We have previously demonstrated that the full-length MFSV nucleoprotein (N) and phosphopro...

  17. Effect of temperature on wheat streak mosaic virus replication and movement in resistant and susceptible wheat cultivars

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV) is an economically important virus causing annual average yield losses of ~2-3% in winter wheat across the Great Plains. Temperature is one of the most important environmental factors that influences disease development and severity. The objective of this study was t...

  18. A possible scenario for the evolution of Banana streak virus in banana.

    PubMed

    Iskra-Caruana, Marie-Line; Chabannes, Matthieu; Duroy, Pierre-Olivier; Muller, Emmanuelle

    2014-06-24

    Outbreaks of Banana streak virus (BSV) have been recorded worldwide where Musa spp. is grown during the last 20 years with no convincing evidence of epidemics. Epidemics were previously reported in Uganda where BSV is currently endemic. BSV is a plant pararetrovirus of the family Caulimoviridae, genus Badnavirus it causes chlorosis leaf streak disease. The information currently available on banana streak disease makes it possible to identify a complex of distinct BSV species each causing the same disease. BSV exists in two states: one as an episomal form, infecting plant cells; the other as viral DNA integrated within the B genome of banana (endogenous BSV-eBSV) forming a viral genome for de novo viral particles. Both forms can be infectious in banana plants. The BSV phylogeny is polyphyletic with BSV distributed in two clades. Clade 1 clusters BSV species that occur worldwide and may have an eBSV counterpart, whereas Clade 3 only comprises BSV species from Uganda. Clearly, two distinct origins explain such BSV diversity. However, the epidemiology/outbreaks of BSV remains unclear and the role of eBSV needs to be clarified. In this review, the biodiversity of BSV is explained and discussed in the light of field and molecular epidemiology data. A scheme is proposed for the co-evolution of BSV and banana based on old or recent infection hypotheses related to African domestication sites and banana dissemination to explain the disease context. PMID:24457073

  19. Quantification of Maize Fine Streak Virus Genomic and Positive-sense RNAs in Infected Maize Reveals High Level Accumulation of ORF 3 and 4 MFSV Transcripts

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Quantification of Maize fine streak virus genomic and positive-sense RNAs in infected maize reveals high level accumulation of ORF 3 and 4 MFSV transcripts. We improved methods to analyze RNA produced by Maize fine streak virus (MVSF) within infected maize tissue using real-time RT-qPCR. We designe...

  20. RNAi-mediated resistance to diverse isolates belonging to two virus species involved in Cassava brown streak disease.

    PubMed

    Patil, Basavaprabhu L; Ogwok, Emmanuel; Wagaba, Henry; Mohammed, Ibrahim U; Yadav, Jitender S; Bagewadi, Basavaraj; Taylor, Nigel J; Kreuze, Jan F; Maruthi, M N; Alicai, Titus; Fauquet, Claude M

    2011-01-01

    Cassava brown streak disease (CBSD) is emerging as one of the most important viral diseases of cassava (Manihot esculenta) and is considered today as the biggest threat to cassava cultivation in East Africa. The disease is caused by isolates of at least two phylogenetically distinct species of single-stranded RNA viruses belonging to the family Potyviridae, genus Ipomovirus. The two species are present predominantly in the coastal lowland [Cassava brown streak virus (CBSV); Tanzania and Mozambique] and highland [Cassava brown streak Uganda virus (CBSUV); Lake Victoria Basin, Uganda, Kenya and Malawi] in East Africa. In this study, we demonstrate that CBSD can be efficiently controlled using RNA interference (RNAi). Three RNAi constructs targeting the highland species were generated, consisting of the full-length (FL; 894 nucleotides), 397-nucleotide N-terminal and 491-nucleotide C-terminal portions of the coat protein (CP) gene of a Ugandan isolate of CBSUV (CBSUV-[UG:Nam:04]), and expressed constitutively in Nicotiana benthamiana. After challenge with CBSUV-[UG:Nam:04], plants homozygous for FL-CP showed the highest resistance, followed by the N-terminal and C-terminal lines with similar resistance. In the case of FL, approximately 85% of the transgenic plant lines produced were completely resistant. Some transgenic lines were also challenged with six distinct isolates representing both species: CBSV and CBSUV. In addition to nearly complete resistance to the homologous virus, two FL plant lines showed 100% resistance and two C-terminal lines expressed 50-100% resistance, whereas the N-terminal lines succumbed to the nonhomologous CBSV isolates. Northern blotting revealed a positive correlation between the level of transgene-specific small interfering RNAs detected in transgenic plants and the level of virus resistance. This is the first demonstration of RNAi-mediated resistance to CBSD and protection across very distant isolates (more than 25% in nucleotide

  1. Mapping the distribution of maize streak virus genotypes across the forest and transition zones of Ghana.

    PubMed

    Oppong, Allen; Offei, Samuel K; Ofori, Kwadwo; Adu-Dapaah, Hans; Lamptey, Joseph N L; Kurenbach, Brigitta; Walters, Matthew; Shepherd, Dionne N; Martin, Darren P; Varsani, Arvind

    2015-02-01

    Throughout sub-Saharan Africa, maize streak virus strain A (MSV-A), the causal agent of maize streak disease (MSD), is an important biological constraint on maize production. In November/December 2010, an MSD survey was carried out in the forest and transition zones of Ghana in order to obtain MSV-A virulence sources for the development of MSD-resistant maize genotypes with agronomic properties suitable for these regions. In 79 well-distributed maize fields, the mean MSD incidence was 18.544 % and the symptom severity score was 2.956 (1 = no symptoms and 5 = extremely severe). We detected no correlation between these two variables. Phylogenetic analysis of cloned MSV-A isolates that were fully sequenced from samples collected in 51 of these fields, together with those sampled from various other parts of Africa, indicated that all of the Ghanaian isolates occurred within a broader cluster of West African isolates, all belonging to the highly virulent MSV-A1 subtype. Besides being the first report of a systematic MSV survey in Ghana, this study is the first to characterize the full-genome sequences of Ghanaian MSV isolates. The 51 genome sequences determined here will additionally be a valuable resource for the rational selection of representative MSV-A variant panels for MSD resistance screening. PMID:25344899

  2. Comparative analysis of virus-derived small RNAs within cassava (Manihot esculenta Crantz) infected with cassava brown streak viruses

    PubMed Central

    Ogwok, Emmanuel; Ilyas, Muhammad; Alicai, Titus; Rey, Marie E.C.; Taylor, Nigel J.

    2016-01-01

    Infection of plant cells by viral pathogens triggers RNA silencing, an innate antiviral defense mechanism. In response to infection, small RNAs (sRNAs) are produced that associate with Argonaute (AGO)-containing silencing complexes which act to inactivate viral genomes by posttranscriptional gene silencing (PTGS). Deep sequencing was used to compare virus-derived small RNAs (vsRNAs) in cassava genotypes NASE 3, TME 204 and 60444 infected with the positive sense single-stranded RNA (+ssRNA) viruses cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the causal agents of cassava brown streak disease (CBSD). An abundance of 21–24 nt vsRNAs was detected and mapped, covering the entire CBSV and UCBSV genomes. The 21 nt vsRNAs were most predominant, followed by the 22 nt class with a slight bias toward sense compared to antisense polarity, and a bias for adenine and uracil bases present at the 5′-terminus. Distribution and frequency of vsRNAs differed between cassava genotypes and viral genomes. In susceptible genotypes TME 204 and 60444, CBSV-derived sRNAs were seen in greater abundance than UCBSV-derived sRNAs. NASE 3, known to be resistant to UCBSV, accumulated negligible UCBSV-derived sRNAs but high populations of CBSV-derived sRNAs. Transcript levels of cassava homologues of AGO2, DCL2 and DCL4, which are central to the gene-silencing complex, were found to be differentially regulated in CBSV- and UCBSV-infected plants across genotypes, suggesting these proteins play a role in antiviral defense. Irrespective of genotype or viral pathogen, maximum populations of vsRNAs mapped to the cytoplasmic inclusion, P1 and P3 protein-encoding regions. Our results indicate disparity between CBSV and UCBSV host-virus interaction mechanisms, and provide insight into the role of virus-induced gene silencing as a mechanism of resistance to CBSD. PMID:26811902

  3. Transcriptome of the plant virus vector Graminella nigrifrons, and the molecular interactions of Maize fine streak rhabdovirus transmission

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: Leafhoppers (Hemiptera:Cicadellidae) are plant-phloem feeders that are known for their ability to vector plant pathogens. The black-faced leafhopper (Graminella nigrifrons) has been identified as the only known vector for the Maize fine streak virus (MFSV), an emerging plant pathogen in...

  4. Genomic and biological characterization of Tomato necrotic streak virus, a novel subgroup 2 ilarvirus infecting tomato in Florida

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tomato necrotic streak virus (TomNSV) is a recently described ilarvirus that was detected in tomato in Florida. The full TomNSV genome sequence revealed it to be a novel subgroup 2 ilarvirus with little nucleotide identity to other previously reported tomato-infecting ilarviruses. Experimental hos...

  5. Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pyramiding of alien-derived Wheat streak mosaic virus (WSMV) resistance and resistance enhancing genes in wheat is a costeffective and environmentally safe strategy for disease control. PCR-based markers and cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chrom...

  6. Effects of wheat streak mosaic virus on root development and water-use efficiency of hard red winter wheat

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Greenhouse and field studies were conducted to determine the effects of Wheat streak mosaic virus (WSMV), a member of the family Potyviridae, on root development and water-use efficiency (WUE) of two hard red winter wheat (Triticum aestivum) cultivars, one susceptible and one resistant to WSMV. In t...

  7. Deletions in the coat protein cistron of wheat streak mosaic virus induced more severe symptoms than the wild-type virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV), an economically important virus in the Great Plains region, is the type member of Tritimovirus genus of the family Potyviridae. The role of coat protein (CP) in WSMV biology was examined by introducing a series of deletions covering SGSGS motifs (aa 36-40, 43-47 an...

  8. Hairpin RNA Targeting Multiple Viral Genes Confers Strong Resistance to Rice Black-Streaked Dwarf Virus

    PubMed Central

    Wang, Fangquan; Li, Wenqi; Zhu, Jinyan; Fan, Fangjun; Wang, Jun; Zhong, Weigong; Wang, Ming-Bo; Liu, Qing; Zhu, Qian-Hao; Zhou, Tong; Lan, Ying; Zhou, Yijun; Yang, Jie

    2016-01-01

    Rice black-streaked dwarf virus (RBSDV) belongs to the genus Fijivirus in the family of Reoviridae and causes severe yield loss in rice-producing areas in Asia. RNA silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering virus resistance in plants, including rice. In this study, we generated transgenic rice lines harbouring a hairpin RNA (hpRNA) construct targeting four RBSDV genes, S1, S2, S6 and S10, encoding the RNA-dependent RNA polymerase, the putative core protein, the RNA silencing suppressor and the outer capsid protein, respectively. Both field nursery and artificial inoculation assays of three generations of the transgenic lines showed that they had strong resistance to RBSDV infection. The RBSDV resistance in the segregating transgenic populations correlated perfectly with the presence of the hpRNA transgene. Furthermore, the hpRNA transgene was expressed in the highly resistant transgenic lines, giving rise to abundant levels of 21–24 nt small interfering RNA (siRNA). By small RNA deep sequencing, the RBSDV-resistant transgenic lines detected siRNAs from all four viral gene sequences in the hpRNA transgene, indicating that the whole chimeric fusion sequence can be efficiently processed by Dicer into siRNAs. Taken together, our results suggest that long hpRNA targeting multiple viral genes can be used to generate stable and durable virus resistance in rice, as well as other plant species. PMID:27187354

  9. Hairpin RNA Targeting Multiple Viral Genes Confers Strong Resistance to Rice Black-Streaked Dwarf Virus.

    PubMed

    Wang, Fangquan; Li, Wenqi; Zhu, Jinyan; Fan, Fangjun; Wang, Jun; Zhong, Weigong; Wang, Ming-Bo; Liu, Qing; Zhu, Qian-Hao; Zhou, Tong; Lan, Ying; Zhou, Yijun; Yang, Jie

    2016-01-01

    Rice black-streaked dwarf virus (RBSDV) belongs to the genus Fijivirus in the family of Reoviridae and causes severe yield loss in rice-producing areas in Asia. RNA silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering virus resistance in plants, including rice. In this study, we generated transgenic rice lines harbouring a hairpin RNA (hpRNA) construct targeting four RBSDV genes, S1, S2, S6 and S10, encoding the RNA-dependent RNA polymerase, the putative core protein, the RNA silencing suppressor and the outer capsid protein, respectively. Both field nursery and artificial inoculation assays of three generations of the transgenic lines showed that they had strong resistance to RBSDV infection. The RBSDV resistance in the segregating transgenic populations correlated perfectly with the presence of the hpRNA transgene. Furthermore, the hpRNA transgene was expressed in the highly resistant transgenic lines, giving rise to abundant levels of 21-24 nt small interfering RNA (siRNA). By small RNA deep sequencing, the RBSDV-resistant transgenic lines detected siRNAs from all four viral gene sequences in the hpRNA transgene, indicating that the whole chimeric fusion sequence can be efficiently processed by Dicer into siRNAs. Taken together, our results suggest that long hpRNA targeting multiple viral genes can be used to generate stable and durable virus resistance in rice, as well as other plant species. PMID:27187354

  10. The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus).

    PubMed

    Su, Li; Li, Zhengnan; Bernardy, Mike; Wiersma, Paul A; Cheng, Zhihui; Xiang, Yu

    2015-10-01

    Pea streak virus (PeSV) is a member of the genus Carlavirus in the family Betaflexiviridae. Here, the first complete genome sequence of PeSV was determined by deep sequencing of a cDNA library constructed from dsRNA extracted from a PeSV-infected sample and Rapid Amplification of cDNA Ends (RACE) PCR. The PeSV genome consists of 8041 nucleotides excluding the poly(A) tail and contains six open reading frames (ORFs). The putative peptide encoded by the PeSV ORF6 has an estimated molecular mass of 6.6 kDa and shows no similarity to any known proteins. This differs from typical carlaviruses, whose ORF6 encodes a 12- to 18-kDa cysteine-rich nucleic-acid-binding protein. PMID:26092422

  11. Detection, discrimination and discovery of a new Tobacco streak virus strain.

    PubMed

    Dutta, M; Ali, A; Melcher, U

    2015-09-01

    Soybean plants that exhibited symptoms of virus infection were sampled from different counties of Oklahoma. These plants were tested serologically for 15 viruses known to infect soybean plants. Fifty-seven samples that exhibited typical virus-like symptoms did not test positive for any of the 15 viruses used in a dot-immunobinding assay (DIBA). Four samples were pooled and used for next generation sequencing using the 454-Roche protocol. Sequence and phylogenetic analysis of the sequences obtained revealed infection with a distinct strain of Tobacco streak virus (TSV). TSV was one of the 15 viruses initially tested for using DIBA and had tested negative. TSV belongs to the genus Ilarvirus and has been reported as a causal agent of bud blight in soybean crops in Brazil and the United States. Out of 10 reported primer pairs for TSV reverse transcription-polymerase chain reaction (RT-PCR), only two had the potential, based on sequence similarity, to amplify part of the genome of the distinct strain of TSV found in Oklahoma and only one was actually able to amplify the region. In this study, a new primer pair, specific to all known TSV and capable of amplifying the Oklahoma strain (TSV-OK), was designed from a highly conserved region of coat protein (CP) sequences and end-point PCR and quantitative RT-PCR detection methods were developed and their sensitivity assayed. This is the first report of specific primers designed from this highly conserved region in the CP of TSV for detection of TSV. Twenty-three of the 57 DIBA soybean samples that initially tested negative were retested with the new specific end-point PCR method and found positive for TSV infection. PMID:25907471

  12. Comparing the regional epidemiology of the cassava mosaic and cassava brown streak virus pandemics in Africa.

    PubMed

    Legg, J P; Jeremiah, S C; Obiero, H M; Maruthi, M N; Ndyetabula, I; Okao-Okuja, G; Bouwmeester, H; Bigirimana, S; Tata-Hangy, W; Gashaka, G; Mkamilo, G; Alicai, T; Lava Kumar, P

    2011-08-01

    The rapid geographical expansion of the cassava mosaic disease (CMD) pandemic, caused by cassava mosaic geminiviruses, has devastated cassava crops in 12 countries of East and Central Africa since the late 1980s. Region-level surveys have revealed a continuing pattern of annual spread westward and southward along a contiguous 'front'. More recently, outbreaks of cassava brown streak disease (CBSD) were reported from Uganda and other parts of East Africa that had been hitherto unaffected by the disease. Recent survey data reveal several significant contrasts between the regional epidemiology of these two pandemics: (i) severe CMD radiates out from an initial centre of origin, whilst CBSD seems to be spreading from independent 'hot-spots'; (ii) the severe CMD pandemic has arisen from recombination and synergy between virus species, whilst the CBSD pandemic seems to be a 'new encounter' situation between host and pathogen; (iii) CMD pandemic spread has been tightly linked with the appearance of super-abundant Bemisia tabaci whitefly vector populations, in contrast to CBSD, where outbreaks have occurred 3-12 years after whitefly population increases; (iv) the CMGs causing CMD are transmitted in a persistent manner, whilst the two cassava brown streak viruses appear to be semi-persistently transmitted; and (v) different patterns of symptom expression mean that phytosanitary measures could be implemented easily for CMD but have limited effectiveness, whereas similar measures are difficult to apply for CBSD but are potentially very effective. An important similarity between the pandemics is that the viruses occurring in pandemic-affected areas are also found elsewhere, indicating that contrary to earlier published conclusions, the viruses per se are unlikely to be the key factors driving the two pandemics. A diagrammatic representation illustrates the temporal relationship between B. tabaci abundance and changing incidences of both CMD and CBSD in the Great Lakes region

  13. Structural studies on tobacco streak virus coat protein: Insights into the pleomorphic nature of ilarviruses.

    PubMed

    Gulati, Ashutosh; Alapati, Kavitha; Murthy, Abhinandan; Savithri, H S; Murthy, M R N

    2016-02-01

    Tobacco streak virus (TSV), the type member of Ilarvirus genus, is a major plant pathogen. TSV purified from infected plants consists of a ss-RNA genome encapsidated in spheroidal particles with diameters of 27, 30 and 33nm constructed from multiple copies of a single species of coat protein (CP) subunits. Apart from protecting the viral genome, CPs of ilarviruses play several key roles in the life cycle of these viruses. Unlike the related bromo and cucumoviruses, ilarvirus particles are labile and pleomorphic, which has posed difficulties in their crystallization and structure determination. In the current study, a truncated TSV-CP was crystallized in two distinct forms and their structures were determined at resolutions of 2.4Å and 2.1Å, respectively. The core of TSV CP was found to possess the canonical β-barrel jelly roll tertiary structure observed in several other viruses. Dimers of CP with swapped C-terminal arms (C-arm) were observed in both the crystal forms. The C-arm was found to be flexible and is likely to be responsible for the polymorphic and pleomorphic nature of TSV capsids. Consistent with this observation, mutations in the hinge region of the C-arm that reduce the flexibility resulted in the formation of more uniform particles. TSV CP was found to be structurally similar to that of Alfalfa mosaic virus (AMV) accounting for similar mechanism of genome activation in alfamo and ilar viruses. This communication represents the first report on the structure of the CP from an ilarvirus. PMID:26706030

  14. Computer analysis between nucleotide and amino acid sequences of bean golden mosaic virus and those of maize streak, wheat dwarf, chloris striate mosaic, and beet curly top viruses.

    PubMed

    Ikegami, M

    1989-01-01

    Bean golden mosaic virus (BGMV) DNA 1 and 2 have little sequence homology with maize streak virus (MSV), wheat dwarf virus (WDV), and chloris striate mosaic virus (CSMV) DNAs. BGMV DNA 1 and beet curly top virus (BCTV) DNA are closely related, whereas BGMV DNA 2 and BCTV DNA are not related. Direct amino acid homologies of predicted proteins between BGMV ORFs and MSV ORFs, WDV ORFs or CSMV ORFs were 40-50%. BGMV 1L1 and BCTV L1, and BGMV IL3 and BCTV L4 were highly conserved. The sequence TAATATTAC was detected in the loops of hairpin structures of 5 gemini-viruses. PMID:2615677

  15. The c-terminus of wheat streak mosaic virus coat protein is involved in differential infection of wheat and maize through host-specific long-distance transport

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Multifunctional viral coat proteins (CPs) play important roles in the virus life-cycle. The CP determinants and mechanisms involved in extension of host range of monocot-infecting viruses are poorly understood. The role of the C-terminal region of Wheat streak mosaic virus (WSMV) CP in virus transpo...

  16. Improved detection of episomal Banana streak viruses by multiplex immunocapture PCR.

    PubMed

    Le Provost, Grégoire; Iskra-Caruana, Marie-Line; Acina, Isabelle; Teycheney, Pierre-Yves

    2006-10-01

    Banana streak viruses (BSV) are currently the main viral constraint to Musa germplasm movement, genetic improvement and mass propagation. Therefore, it is necessary to develop and implement BSV detection strategies that are both reliable and sensitive, such as PCR-based techniques. Unfortunately, BSV endogenous pararetrovirus sequences (BSV EPRVs) are present in the genome of Musa balbisiana. They interfere with PCR-based detection of episomal BSV in infected banana and plantain, such as immunocapture PCR. Therefore, a multiplex, immunocapture PCR (M-IC-PCR) was developed for the detection of BSV. Musa sequence tagged microsatellite site (STMS) primers were selected and used in combination with BSV species-specific primers in order to monitor possible contamination by Musa genomic DNA, using multiplex PCR. Furthermore, immunocapture conditions were optimized in order to prevent Musa DNA from interfering with episomal BSV DNA during the PCR step. This improved detection method successfully allowed the accurate, specific and sensitive detection of episomal DNA only from distinct BSV species. Its implementation should benefit PCR-based detection of viruses for which homologous sequences are present in the genome of their hosts, including transgenic plants expressing viral sequences. PMID:16857272

  17. Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus

    PubMed Central

    2012-01-01

    Background Single-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus). Results Although both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV

  18. Comparative reaction of popular high yielding spanish and virginia bunch groundnut varieties to Tobacco streak virus.

    PubMed

    Vemana, K; Jain, R K

    2013-09-01

    Reaction of five spanish (JL 24, TMV 2, Kadiri 6, Kadiri 9 and Anantha) and a virginia (Kadiri 7 Bold) groundnut cultivars was studied against Tobacco streak virus (TSV) by sap inoculation using different age group of plants [7-84 days after sowing (DAS)]. Among different cultivars, incubation period varied from 4 to 28 days and high incubation period recorded in Kadiri 7 Bold. The percent infection decreased with increase in the age of the plants in all the cultivars as it ranged up to 100 % in both pre-flowering (7-21 DAS) and flowering stage (28-63 DAS) plants followed by 50-100 % in maturity stage plants (70-84 DAS) except Kadiri 7 Bold. Similarly, 100 % wilting was observed in pre flowering stage plants against no wilting in maturity stage plants. However, cultivars differed in per cent wilting of flowering stage plants by recording maximum wilting (100 %) in JL24, Kadiri 6 and minimum (25.0 %) in Kadiri 7 Bold. Both localized (necrotic spots, veinal necrosis) and systemic (petiole necrosis, necrotic spots on young leaves, top growing bud and stem necrosis, axillary shoot proliferation, stunting, peg necrosis, pod necrosis, wilting of plant) symptoms induced by TSV were similar among all cultivars without any new symptoms. Prolonged stage of axillary shoot proliferation was observed for the first time in all the cultivars. In maturity stage plants of Kadiri 7 Bold, Kadiri 9 and Anantha, systemic symptoms restricted to leaf and petiole necrosis only. Virus titer varied significantly with the age of plants and inoculum harvest at days post inoculation and least virus titer recorded by Kadiri 7 Bold at all stages of infection. Among different cultivars, Kadiri 7 Bold was least susceptible/tolerant to TSV by registering higher incubation period with less per cent infection, wilt and titer. PMID:24426278

  19. Amino acid substitutions of cysteine residues near the amino terminus of Wheat streak mosaic virus HC-Pro abolishes virus transmission by the wheat curl mite

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The amino-terminal half of HC-Pro of Wheat streak mosaic virus (WSMV) is required for semi-persistent transmission by the wheat curl mite (Aceria tosichella Keifer). The amino-proximal region of WSMV HC-Pro is cysteine-rich with a zinc finger-like motif. Amino acid substitutions were made in this re...

  20. Substitution of conserved cysteine residues in Wheat streak mosaic virus HC-Pro abolishes virus transmission by the wheat curl mite

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Substitutions in the amino-terminal region of Wheat streak mosaic virus (WSMV) HC-Pro were evaluated for effects on transmission by the wheat curl mite (Aceria tosichella Keifer). Alanine substitution at cysteine residues 16, 46 and 49 abolished vector transmission. Although alanine substitution a...

  1. New Experimental Hosts of Tobacco streak virus and Absence of True Seed Transmission in Leguminous Hosts.

    PubMed

    Vemana, K; Jain, R K

    2010-10-01

    Of 70 plant species tested, 50 species were susceptible to Tobacco streak virus (TSV) on sap inoculation. Both localized (necrotic and chlorotic spots) and systemic (necrotic spots, axillary shoot proliferation, stunting, total necrosis and wilt) symptoms are observed by majority of plant species. Eleven new experimental hosts were identified viz., Amaranthus blitum var. oleracea (Chaulai sag), Celosia cristata (Cocks comb), Beta vulgaris var. bengalensis (Palak/Indian spinach), Calendula officinalis (Pot marigold), Chrysanthemum indicum, Cosmos sulphurens (Yellow cosmos), Citrullus lunatus (Watermelon), Lagenaria siceraria (Bottle gourd), Coriandrum sativum (Coriander), Hibiscus subderiffa var. subderiffa (Roselle) and Portulaca oleraceae (Little hogweed). Detected groundnut seed infection with TSV for the first time by Direct antigen coated immunosorbent assay (DAC-ELISA) using whole seed. The seed infection ranged from 18.9 to 28.9% among the seeds collected from naturally infected and sap inoculated groundnut varieties (JL 24, TMV 2, Prasuna, Kadiri 6, Kadiri 9, Anantha and Kadiri 7 Bold) belonging to spanish and virginia types. Further, TSV was detected both in pod shell and seed testa and none of the samples showed the presence of TSV either in cotyledon or embryo. Grow-out and bio-assay tests proved the absence of seed transmission in groundnut and other legume crops. Hence, TSV isolate was not a true seed transmission case under Indian conditions in legumes. PMID:23637490

  2. Replication and encapsidation of the viroid-like satellite RNA of lucerne transient streak virus are supported in divergent hosts by cocksfoot mottle virus and turnip rosette virus.

    PubMed

    Sehgal, O P; Sinha, R C; Gellatly, D L; Ivanov, I; AbouHaidar, M G

    1993-04-01

    Cocksfoot mottle sobemovirus supports replication and encapsidation of the viroid-like satellite RNA (sat-RNA) of lucerne transient streak virus (LTSV) in two monocotyledonous species, Triticum aestivum and Dactylis glomerata. Additionally, LTSV sat-RNA replicates effectively in the presence of turnip rosette sobemovirus in Brassica rapa, Raphanus raphanistrum and Sinapsis arvensis, but not in Thlaspi arvense or Nicotiana bigelovii, indicating that host species markedly influence this interaction. Previous reports of the association between LTSV sat-RNA and helper sobemoviruses were limited to dicotyledonous hosts. Our results demonstrate that the biological interaction between these two entities spans divergent dicotyledonous and monocotyledonous species. PMID:7682254

  3. Method: a single nucleotide polymorphism genotyping method for Wheat streak mosaic virus

    PubMed Central

    2012-01-01

    Background The September 11, 2001 attacks on the World Trade Center and the Pentagon increased the concern about the potential for terrorist attacks on many vulnerable sectors of the US, including agriculture. The concentrated nature of crops, easily obtainable biological agents, and highly detrimental impacts make agroterrorism a potential threat. Although procedures for an effective criminal investigation and attribution following such an attack are available, important enhancements are still needed, one of which is the capability for fine discrimination among pathogen strains. The purpose of this study was to develop a molecular typing assay for use in a forensic investigation, using Wheat streak mosaic virus (WSMV) as a model plant virus. Method This genotyping technique utilizes single base primer extension to generate a genetic fingerprint. Fifteen single nucleotide polymorphisms (SNPs) within the coat protein and helper component-protease genes were selected as the genetic markers for this assay. Assay optimization and sensitivity testing was conducted using synthetic targets. WSMV strains and field isolates were collected from regions around the world and used to evaluate the assay for discrimination. The assay specificity was tested against a panel of near-neighbors consisting of genetic and environmental near-neighbors. Result Each WSMV strain or field isolate tested produced a unique SNP fingerprint, with the exception of three isolates collected within the same geographic location that produced indistinguishable fingerprints. The results were consistent among replicates, demonstrating the reproducibility of the assay. No SNP fingerprints were generated from organisms included in the near-neighbor panel, suggesting the assay is specific for WSMV. Using synthetic targets, a complete profile could be generated from as low as 7.15 fmoles of cDNA. Conclusion The molecular typing method presented is one tool that could be incorporated into the forensic

  4. Physiological Responses of Hard Red Winter Wheat to Infection by Wheat streak mosaic virus.

    PubMed

    Pradhan, Gautam P; Xue, Qingwu; Jessup, Kirk E; Hao, Baozhen; Price, Jacob A; Rush, Charles M

    2015-05-01

    Wheat streak mosaic virus (WSMV) causes significant yield loss in hard red winter wheat in the U.S. Southern High Plains. Despite the prevalence of this pathogen, little is known about the physiological response of wheat to WSMV infection. A 2-year study was initiated to (i) investigate the effect of WSMV, inoculated at different development stages, on shoot and root growth, water use, water use efficiency (WUE), and photosynthesis and (ii) understand the relationships between yield and photosynthetic parameters during WSMV infection. Two greenhouse experiments were conducted with two wheat cultivars mechanically inoculated with WSMV at different developmental stages, from three-leaf to booting. WSMV inoculated early, at three- to five-leaf stage, resulted in a significant reduction in shoot biomass, root dry weight, and yield compared with wheat infected at the jointing and booting stages. However, even when inoculated as late as jointing, WSMV still reduced grain yield by at least 53%. Reduced tillers, shoot biomass, root dry weight, water use, and WUE contributed to yield loss under WSMV infection. However, infection by WSMV did not affect rooting depth and the number of seminal roots but reduced the number of nodal roots. Leaf photosynthetic parameters (chlorophyll [SPAD], net photosynthetic rate [Pn], stomatal conductance [Gs], intercellular CO2 concentration [Ci], and transpiration rate [Tr]) were reduced when infected by WSMV, and early infection reduced parameters more than late infection. Photosynthetic parameters had a linear relationship with grain yield and shoot biomass. The reduced Pn under WSMV infection was mainly in response to decreased Gs, Ci, and SPAD. The results of this study indicated that leaf chlorophyll and gas exchange parameters can be used to quantify WSMV effects on biomass and grain yield in wheat. PMID:25901871

  5. Subpopulation level variation of banana streak viruses in India and common evolution of banana and sugarcane badnaviruses.

    PubMed

    Sharma, Susheel Kumar; Vignesh Kumar, P; Geetanjali, A Swapna; Pun, Khem Bahadur; Baranwal, Virendra Kumar

    2015-06-01

    Genome sequences of three episomal Banana streak MY virus (BSMYV) isolates sampled from triploid banana hybrids (Chini Champa: AAB; Malbhog: AAB and Monthan: ABB), grown in North-East and South India are reported in this study by sequence-independent improved rolling circle amplification (RCA). RCA coupled with restriction fragment length polymorphism revealed diverse restriction profiles of five BSMYV isolates. Nucleotide substitution rates of BSMYV subpopulation and Banana streak OL virus subpopulation was 7.13 × 10(-3) to 1.59 × 10(-2) and 2.65 × 10(-3) to 5.49 × 10(-3), respectively, for the different coding regions. Analysis of the genetic diversity of banana and sugarcane badnaviruses revealed a total of 32 unique recombination events among banana and sugarcane badnaviruses (inter BSV-SCBV), in addition to the extensive recombination with in banana streak viruses and sugarcane bacilliform viruses (intra-BSV and intra-SCBV). Many unique fragments were shown to contain similar ruminant sequence fragments which indicated the possibility that the two groups of badnaviruses or their ancestors to colonise same host before making the host shift. The distribution of recombination events, hot-spots (intergenic region and C-terminal of ORF3) as well as cold-spots (distributed in ORF3) displayed the mirroring of recombination traces in both group of badnaviruses. These results support the hypothesis of relatedness of banana and sugarcane badnaviruses and the host and geographical shifts that followed the fixation of the species complex appear to be a recent event. PMID:25672291

  6. Complete genome sequence of nine isolates of canna yellow streak virus reveals its relationship to the sugarcane mosaic virus (SCMV) subgroup of potyviruses.

    PubMed

    Chauhan, Ravendra P; Rajakaruna, Punsasi; Verchot, Jeanmarie

    2015-03-01

    Complete genome sequences were obtained from nine isolates of canna yellow streak virus (CaYSV). CaYSV belongs to the sugarcane mosaic virus (SCMV) subgroup of potyviruses with johnsongrass mosaic virus (JGMV) as its closest relative. Multiple sequence alignments showed a pattern of amino acid substitutions in the CP sequences, which enabled us to relate these isolates to South East Asian or European isolates. Biological characterization of CaYSV identified Nicotiana benthamiana, Chenopodium quinoa and Phaseolus vulgaris as experimental hosts. Given the popularity and global trade of cannas, a clear picture of the genetic diversity of CaYSV is critical to disease management. PMID:25567205

  7. Genomic variability and molecular evolution of Asian isolates of sugarcane streak mosaic virus.

    PubMed

    Liang, Shan-Shan; Alabi, Olufemi J; Damaj, Mona B; Fu, Wei-Lin; Sun, Sheng-Ren; Fu, Hua-Ying; Chen, Ru-Kai; Mirkov, T Erik; Gao, San-Ji

    2016-06-01

    Sugarcane streak mosaic virus (SCSMV), an economically important causal agent of mosaic disease of sugarcane, is a member of the newly created genus Poacevirus in the family Potyviridae. In this study, we report the molecular characterization of three new SCSMV isolates from China (YN-YZ211 and HN-YZ49) and Myanmar (MYA-Formosa) and their genetic variation and phylogenetic relationship to SCSMV isolates from Asia and the type members of the family Potyviridae. The complete genome of each of the three isolates was determined to be 9781 nucleotides (nt) in size, excluding the 3' poly(A) tail. Phylogenetic analysis of the complete polyprotein amino acid (aa) sequences (3130 aa) revealed that all SCSMV isolates clustered into a phylogroup specific to the genus Poacevirus and formed two distinct clades designated as group I and group II. Isolates YN-YZ211, HN-YZ49 and MYA-Formosa clustered into group I, sharing 96.8-99.5 % and 98.9-99.6 % nt (at the complete genomic level) and aa (at the polyprotein level) identity, respectively, among themselves and 81.2-98.8 % and 94.0-99.6 % nt (at the complete genomic level) and aa (at the polyprotein level) identity, respectively, with the corresponding sequences of seven Asian SCSMV isolates. Population genetic analysis revealed greater between-group (0.190 ± 0.004) than within-group (group I = 0.025 ± 0.001 and group II = 0.071 ± 0.003) evolutionary divergence values, further supporting the results of the phylogenetic analysis. Further analysis indicated that natural selection might have contributed to the evolution of isolates belonging to the two identified SCSMV clades, with infrequent genetic exchanges occurring between them over time. These findings provide a comprehensive analysis of the population genetic structure and driving forces for the evolution of SCSMV with implications for global exchange of sugarcane germplasm. PMID:26973230

  8. Quantitative detection of relative expression levels of the whole genome of Southern rice black-streaked dwarf virus and its replication in different hosts

    PubMed Central

    2013-01-01

    Background In recent years, a disease caused by Southern rice black-streaked dwarf virus (SRBSDV) has resulted in significant loss in rice production in Southern China and has spread quickly throughout East and Southeast Asia. This virus is transmitted by an insect vector, white-backed planthopper (WBPH) Sogatella furcifera (Hemiptera: Delphacidae), in a persistent propagative manner. Aside from rice, SRBSDV can also infect numerous Poaceae plants. However, the molecular mechanism of interaction between SRBSDV and its plant or insect vector remains unclear. In order to address this, we investigated the whole viral genome relative mRNA expression level in distinct hosts and monitored their expression level in real-time in rice plants. Methods In this study, a reliable, rapid, and sensitive method for detecting viral gene expression transcripts is reported. A SYBR Green I based real-time polymerase chain reaction (PCR) method was adopted for the quantitative detection of SRBSDV gene expression in different hosts and real-time changes in gene expression in rice. Results Compared to the relative mRNA expression level of the whole genome of SRBSDV, P3, P7-1, and P9-2 were dominantly expressed in rice and WBPH. Similarly, these genes also exhibited high expression levels in corn, suggesting that they have more important functions than other viral genes in the interaction between SRBSDV and hosts, and that they could be used as molecular detection target genes of SRBSDV. In contrast, the levels of P6 and P10 were relative low. Western blotting analysis partially was also verified our qPCR results at the level of protein expression. Analysis of the real-time changes in SRBSDV-infected rice plants revealed four distinct temporal expression patterns of the thirteen genes. Moreover, expression levels of P1 and other genes were significantly down-regulated on days 14 and 20, respectively. Conclusion SRBSDV genes showed similar expression patterns in distinct hosts (rice, corn

  9. Strand-specific real-time RT-PCR quantitation of Maize fine streak virus genomic and positive-sense RNAs using high temperature reverse transcription

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Efforts to analyze the replicative RNA produced by Maize fine streak virus (MFSV) within maize tissue was complicated by the lack of specificity during cDNA generation using standard reverse transcriptase protocols. Real-time qRT-PCR using cDNA generated by priming with random hexamers does not dist...

  10. Strand-specific real-time RT-PCR quantitation of Maize fine streak virus genomic and positive-sense RNAs using high temperature reverse transcription

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Efforts to analyze the replicative RNA produced by Maize fine streak virus (MVSF) within maize tissue was complicated by the lack of specificity during cDNA generation using standard reverse transcriptase protocols. Real-time qRT-PCR using cDNA generated by priming with random hexamers does not dist...

  11. Low Temperature Storage of Southern Rice Black-Streaked Dwarf Virus-Infected Rice Plants Cannot Sustain Virus Transmission by the Vector.

    PubMed

    Liu, Danfeng; Li, Pei; Han, Yongqiang; Lei, Wenbin; Hou, Maolin

    2016-02-01

    Southern rice black-streaked dwarf virus (SRBSDV) is a novel virus transmitted by white-backed planthopper Sogatella furcifera (Hováth) (Hemiptera: Delphacidae). Due to low virus transmission efficiency by the planthopper, researchers are frequently confronted with shortage of viruliferous vectors or infected rice plants, especially in winter and the following spring. To find new ways to maintain virus-infected materials, viral rice plants were stored at -80°C for 45 or 140 d and evaluated as virus sources in virus transmission by the vector. SRBSDV virions were not degraded during storage at -80°C as indicated by reverse transcription-polymerase chain reaction and reverse transcription real-time PCR detection. The planthopper nymphs fed on the infected thawed plants for 48 h survived at about 40% and showed positive detection of SRBSDV, but they lost the virus after feeding for another 20 d (the circulative transmission period) on noninfected plants. Transmission electron microscope images indicated broken capsid of virions in infected thawed leaves in contrast to integrity capsid of virions in infected fresh leaves. These results show that low temperature storage of SRBSDV-infected rice plants cannot sustain virus transmission by white-backed planthopper. PMID:26405060

  12. Characterization of the Triticum Mosaic Virus Genome and Interactions between Triticum Mosaic Virus and Wheat Streak Mosaic Virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The complete genome sequence of Triticum mosaic virus (TriMV) has been determined to be 10,266 nucleotides encoding a large polyprotein of 3,112 amino acids. The proteins of TriMV possess only 33-44% (with NIb protein) and 15-29% (with P1 protein) amino acid identity with the reported members of Pot...

  13. Efficient and stable expression of GFP through Wheat streak mosaic virus-based vectors in cereal hosts using a range of cleavage sites: Formation of dense fluorescent aggregates for sensitive virus tracking

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A series of Wheat streak mosaic virus (WSMV)-based expression vectors were developed by engineering cycle 3 GFP (GFP) cistron between P1 and HC-Pro cistrons with several catalytic/cleavage peptides at the C-terminus of GFP. WSMV-GFP vectors with the Foot-and-mouth disease virus 1D/2A or 2A catalytic...

  14. [Corn.

    ERIC Educational Resources Information Center

    Iowa History for Young People, 1993

    1993-01-01

    This theme issue focuses on corn. Iowa is the number one corn producing state in the United States. The featured articles in the issue concern, among other topics, Iowa children who live on farms, facts and statistics about corn, the Mesquakie Indians and corn shelling, corn hybrids, a short story, and the corn palaces of Sioux City. Activities,…

  15. Genetic insights into Graminella nigrifrons competence for Maize fine streak virus infection and transmission

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insects are critical for the spread of most plant virus diseases, with >75% of plant viruses depending on an insects for transmission to new, uninfected hosts. However, little is known about the molecular and cellular factors in the insect that are important for virus transmission. The black-faced l...

  16. Analysis of the distribution and structure of integrated Banana streak virus DNA in a range of Musa cultivars.

    PubMed

    Geering, A D; Olszewski, N E; Dahal, G; Thomas, J E; Lockhart, B E

    2001-07-01

    Summary Banana streak virus strain OL (BSV-OL) commonly infects new Musa hybrids, and this infection is thought to arise de novo from integrated virus sequences present in the nuclear genome of the plant. Integrated DNA (Musa6+8 sequence) containing the whole genome of the virus has previously been cloned from cv. Obino l'Ewai (Musa AAB group), a parent of many of the hybrids. Using a Southern blot hybridization assay, we have examined the distribution and structure of integrated BSV-OL sequences in a range of Musa cultivars. For cv. Obino l'Ewai, almost every restriction fragment hybridizing to BSV-OL was predicted from the Musa6+8 sequence, suggesting that this is the predominant type of BSV-OL integrant in the genome. Furthermore, since only two junction fragments of Musa/BSV sequence were detected, and the Musa6+8 sequence is believed to be integrated as multiple copies in a tandem array, then the internal Musa spacer sequences must be highly conserved. Similarly sized restriction fragments were detected in four BB group cultivars, but not in six AA or AAA group cultivars, suggesting that the BSV-OL sequences are linked to the B-genome of Musa. We also provide evidence that cv. Williams (Musa AAA group) contains a distinct badnavirus integrant that is closely related to the 'dead' virus integrant previously characterized from Calcutta 4 (Musa acuminata ssp. burmannicoides). Our results suggest that the virus integrant from cv. Williams is linked to the A-genome, and the complexity of the hybridization patterns suggest multiple sites of integration and/or variation in sequence and structure of the integrants. PMID:20573008

  17. Recombination, decreased host specificity and increased mobility may have driven the emergence of maize streak virus as an agricultural pathogen.

    PubMed

    Varsani, Arvind; Shepherd, Dionne N; Monjane, Adérito L; Owor, Betty E; Erdmann, Julia B; Rybicki, Edward P; Peterschmitt, Michel; Briddon, Rob W; Markham, Peter G; Oluwafemi, Sunday; Windram, Oliver P; Lefeuvre, Pierre; Lett, Jean-Michel; Martin, Darren P

    2008-09-01

    Maize streak virus (MSV; family Geminiviridae, genus Mastrevirus), the causal agent of maize streak disease, ranks amongst the most serious biological threats to food security in subSaharan Africa. Although five distinct MSV strains have been currently described, only one of these - MSV-A - causes severe disease in maize. Due primarily to their not being an obvious threat to agriculture, very little is known about the 'grass-adapted' MSV strains, MSV-B, -C, -D and -E. Since comparing the genetic diversities, geographical distributions and natural host ranges of MSV-A with the other MSV strains could provide valuable information on the epidemiology, evolution and emergence of MSV-A, we carried out a phylogeographical analysis of MSVs found in uncultivated indigenous African grasses. Amongst the 83 new MSV genomes presented here, we report the discovery of six new MSV strains (MSV-F to -K). The non-random recombination breakpoint distributions detectable with these and other available mastrevirus sequences partially mirror those seen in begomoviruses, implying that the forces shaping these breakpoint patterns have been largely conserved since the earliest geminivirus ancestors. We present evidence that the ancestor of all MSV-A variants was the recombinant progeny of ancestral MSV-B and MSV-G/-F variants. While it remains unknown whether recombination influenced the emergence of MSV-A in maize, our discovery that MSV-A variants may both move between and become established in different regions of Africa with greater ease, and infect more grass species than other MSV strains, goes some way towards explaining why MSV-A is such a successful maize pathogen. PMID:18753214

  18. Whole-genome expression analysis of Rice black-streaked dwarf virus in different plant hosts and small brown planthopper.

    PubMed

    Xu, Qiufang; Ni, Haiping; Zhang, Jinfeng; Lan, Ying; Ren, Chunmei; Zhou, Yijun

    2015-11-10

    Rice black-streaked dwarf virus (RBSDV) can infect a number of gramineous plants and cause severe crop yield losses in southeast Asian countries. The virus is transmitted by small brown planthopper (SBPH) in a persistent circulative manner. The interactions between RBSDV and its different hosts remain unknown. Besides, how the virus adjusts itself to infect different hosts is unclear. In the present study, the relative RNA levels of the thirteen RBSDV genes in rice, maize, wheat, and SBPH were measured by real-time quantitative PCR. P7-1 and P10 genes were predominantly expressed whereas P8 and P7-2 genes were expressed at low levels in plant hosts. Similar to the expression in rice, P7-1 was the most abundantly expressed gene and P8 was expressed at the lowest level in SBPH, indicating that RBSDV adopts the same strategy to infect distinct hosts. The high expression levels of the P7-1 gene in both plants and insect suggest that it can be used as the target gene for disease diagnostics. However, the expression levels of some genes varied from host to host. P5-1, P6 and P9-1, the components of the RBSDV viroplasm, are differentially expressed in different hosts. Moreover, western blot analysis showed that the quantity of the P9-1 protein was more abundant in SBPH than in plant hosts. These data indicate that the virus may adjust its own gene expression to replicate in different hosts. Analysis of time course of gene expression revealed that P7-1 stands out as the only gene highly expressed at the earliest time point and its expression precedes all others throughout infection from 8 to 24days post-inoculation. The high expression levels of the P7-1 gene suggest that it plays a significant role in RBSDV-host interactions. PMID:26149652

  19. GFP is Efficiently Expressed by Wheat Streak Mosaic Virus Using a Range of Tritimovirus NIa Cleavage Sites and Forms Dense Aggregates in Cereal Hosts

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wheat streak mosaic virus (WSMV)-based transient expression vector was developed to express GFP as a marker protein. The GFP cistron was engineered between the P1 and HC-Pro cistrons in an infectious cDNA clone of WSMV. The cleavage sites, P3/6KI, 6KI/CI, NIa/NIb, or NIb/CP, from WSMV were fused to ...

  20. Chryse Streak

    NASA Technical Reports Server (NTRS)

    2006-01-01

    6 May 2006 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a wind streak created in the lee -- the downwind side -- of a crater in far eastern Chryse Planitia. The winds responsible for the formation of the streak blew from the upper right (northeast) to the lower left (southwest).

    Location near: 21.5oN, 27.4oW Image width: 3 km (1.9 mi) Illumination from: lower left Season: Northern Winter

  1. Daedalia Streak

    NASA Technical Reports Server (NTRS)

    2005-01-01

    1 December 2005 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a dark wind streak formed in the lee of a crater in Daedalia Planum. The winds responsible for the streak blew from right (east) to left (west).

    Location near: 11.7oS, 136.4oW Image width: width: 3 km (1.9 mi) Illumination from: lower left Season: Southern Summer

  2. Exploiting the combination of natural and genetically engineered resistance to cassava mosaic and cassava brown streak viruses impacting cassava production in Africa.

    PubMed

    Vanderschuren, Hervé; Moreno, Isabel; Anjanappa, Ravi B; Zainuddin, Ima M; Gruissem, Wilhelm

    2012-01-01

    Cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) are currently two major viral diseases that severely reduce cassava production in large areas of Sub-Saharan Africa. Natural resistance has so far only been reported for CMD in cassava. CBSD is caused by two virus species, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). A sequence of the CBSV coat protein (CP) highly conserved between the two virus species was used to demonstrate that a CBSV-CP hairpin construct sufficed to generate immunity against both viral species in the cassava model cultivar (cv. 60444). Most of the transgenic lines showed high levels of resistance under increasing viral loads using a stringent top-grafting method of inoculation. No viral replication was observed in the resistant transgenic lines and they remained free of typical CBSD root symptoms 7 month post-infection. To generate transgenic cassava lines combining resistance to both CBSD and CMD the hairpin construct was transferred to a CMD-resistant farmer-preferred Nigerian landrace TME 7 (Oko-Iyawo). An adapted protocol allowed the efficient Agrobacterium-based transformation of TME 7 and the regeneration of transgenic lines with high levels of CBSV-CP hairpin-derived small RNAs. All transgenic TME 7 lines were immune to both CBSV and UCBSV infections. Further evaluation of the transgenic TME 7 lines revealed that CBSD resistance was maintained when plants were co-inoculated with East African cassava mosaic virus (EACMV), a geminivirus causing CMD. The innovative combination of natural and engineered virus resistance in farmer-preferred landraces will be particularly important to reducing the increasing impact of cassava viral diseases in Africa. PMID:23049780

  3. Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance.

    PubMed

    Ali, N; Heslop-Harrison, Js Pat; Ahmad, H; Graybosch, R A; Hein, G L; Schwarzacher, T

    2016-08-01

    Pyramiding of alien-derived Wheat streak mosaic virus (WSMV) resistance and resistance enhancing genes in wheat is a cost-effective and environmentally safe strategy for disease control. PCR-based markers and cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin in four genetically diverse populations of wheat (Triticum aestivum) lines incorporating chromosome segments from Thinopyrum intermedium and Secale cereale (rye). Out of 20 experimental lines, 10 carried Th. intermedium chromatin as T4DL*4Ai#2S translocations, while, unexpectedly, 7 lines were positive for alien chromatin (Th. intermedium or rye) on chromosome 1B. The newly described rye 1RS chromatin, transmitted from early in the pedigree, was associated with enhanced WSMV resistance. Under field conditions, the 1RS chromatin alone showed some resistance, while together with the Th. intermedium 4Ai#2S offered superior resistance to that demonstrated by the known resistant cultivar Mace. Most alien wheat lines carry whole chromosome arms, and it is notable that these lines showed intra-arm recombination within the 1BS arm. The translocation breakpoints between 1BS and alien chromatin fell in three categories: (i) at or near to the centromere, (ii) intercalary between markers UL-Thin5 and Xgwm1130 and (iii) towards the telomere between Xgwm0911 and Xbarc194. Labelled genomic Th. intermedium DNA hybridised to the rye 1RS chromatin under high stringency conditions, indicating the presence of shared tandem repeats among the cereals. The novel small alien fragments may explain the difficulty in developing well-adapted lines carrying Wsm1 despite improved tolerance to the virus. The results will facilitate directed chromosome engineering producing agronomically desirable WSMV-resistant germplasm. PMID:27245423

  4. Molecular Genetic Analysis and Evolution of Segment 7 in Rice Black-Streaked Dwarf Virus in China

    PubMed Central

    Chen, Yanping; Wu, Jirong; Meng, Qingchang; Han, Xiaohua; Hao, Zhuanfang; Li, Mingshun; Yong, Hongjun; Zhang, Degui; Zhang, Shihuang; Li, Xinhai

    2015-01-01

    Rice black-streaked dwarf virus (RBSDV) causes maize rough dwarf disease or rice black-streaked dwarf disease and can lead to severe yield losses in maize and rice. To analyse RBSDV evolution, codon usage bias and genetic structure were investigated in 111 maize and rice RBSDV isolates from eight geographic locations in 2013 and 2014. The linear dsRNA S7 is A+U rich, with overall codon usage biased toward codons ending with A (A3s, S7-1: 32.64%, S7-2: 29.95%) or U (U3s, S7-1: 44.18%, S7-2: 46.06%). Effective number of codons (Nc) values of 45.63 in S7-1 (the first open reading frame of S7) and 39.96 in S7-2 (the second open reading frame of S7) indicate low degrees of RBSDV-S7 codon usage bias, likely driven by mutational bias regardless of year, host, or geographical origin. Twelve optimal codons were detected in S7. The nucleotide diversity (π) of S7 sequences in 2013 isolates (0.0307) was significantly higher than in 2014 isolates (0.0244, P = 0.0226). The nucleotide diversity (π) of S7 sequences in isolates from Jinan (0.0391) was higher than that from the other seven locations (P < 0.01). Only one S7 recombinant was detected in Baoding. RBSDV isolates could be phylogenetically classified into two groups according to S7 sequences, and further classified into two subgroups. S7-1 and S7-2 were under negative and purifying selection, with respective Ka/Ks ratios of 0.0179 and 0.0537. These RBSDV populations were expanding (P < 0.01) as indicated by negative values for Tajima's D, Fu and Li's D, and Fu and Li's F. Genetic differentiation was detected in six RBSDV subpopulations (P < 0.05). Absolute Fst (0.0790) and Nm (65.12) between 2013 and 2014, absolute Fst (0.1720) and Nm (38.49) between maize and rice, and absolute Fst values of 0.0085-0.3069 and Nm values of 0.56-29.61 among these eight geographic locations revealed frequent gene flow between subpopulations. Gene flow between 2013 and 2014 was the most frequent. PMID:26121638

  5. [Angioid streaks].

    PubMed

    Matonti, F; Conrath, J

    2012-12-01

    Angioid streaks represent breaks in Bruch's membrane, appearing as dark or reddish radial streaks eminating from the optic disc. Usually asymptomatic, these streaks may develop neovascularisation and lead to a maculopathy with marked loss of vision. Some associations with systemic disease are classically described, especially pseudoxanthoma elasticum. This condition may involve cardiovascular complications. A mutation has been found in the ABCC6 gene, which encodes for a membrane transport protein involved in the synthesis of the extracellular matrix. Imaging allows for visualization of the extent of the streaks, and autofluorescence is particularly informative. Spectral domain OCT may also demonstrate early breaks in Bruch's membrane. Neovascular complications, previously responsible for inevitable visual impairment at some point after their occurrence, are now managed by intravitreal injections of anti-VEGFs with clear efficacity. The ophthalmologist must be aware of this condition, in order to guide the patient towards a systemic work-up if necessary, and also to insure quick and targeted treatment in the case of neovascular complications. PMID:23046745

  6. Wind Streaks

    NASA Technical Reports Server (NTRS)

    2003-01-01

    [figure removed for brevity, see original site]

    Released 17 September 2003

    Bright wind streaks are present in the lee of craters and other obstacles in this image, located in Sinus Sabaeus, near the Martian equator. These streaks indicate that the local winds blow from the northeast (upper right in the image). The brightness of the streaks indicates that either bright material has been deposited in the lee of the craters, or that the surface has eroded preferentially in the lee of craters, exposing an underlying bright material. Because the streaks are bright regardless of the surrounding surface brightness, the first hypothesis most likely. The streaks probably all represent deposits of the same bright material that settled out of the atmosphere in the wind shelter provided by topographic peaks.

    Image information: VIS instrument. Latitude -6.3, Longitude 14.1 East (345.9 West). 19 meter/pixel resolution.

    Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time.

    NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the

  7. NAC transcription factor family genes are differentially expressed in rice during infections with Rice dwarf virus, Rice black-streaked dwarf virus, Rice grassy stunt virus, Rice ragged stunt virus, and Rice transitory yellowing virus

    PubMed Central

    Nuruzzaman, Mohammed; Sharoni, Akhter M.; Satoh, Kouji; Karim, Mohammad Rezaul; Harikrishna, Jennifer A.; Shimizu, Takumi; Sasaya, Takahide; Omura, Toshihiro; Haque, Mohammad A.; Hasan, Sayed M. Z.; Ahmad, Aziz; Kikuchi, Shoshi

    2015-01-01

    Expression levels of the NAC gene family were studied in rice infected with Rice dwarf virus (RDV), Rice black-streaked dwarf virus (RBSDV), Rice grassy stunt virus (RGSV), Rice ragged stunt virus (RRSV), and Rice transitory yellowing virus (RTYV). Microarray analysis showed that 75 (68%) OsNAC genes were differentially regulated during infection with RDV, RBSDV, RGSV, and RRSV compared with the control. The number of OsNAC genes up-regulated was highest during RGSV infection, while the lowest number was found during RTYV infection. These phenomena correlate with the severity of the syndromes induced by the virus infections. Most of the genes in the NAC subgroups NAC22, SND, ONAC2, ANAC34, and ONAC3 were down-regulated for all virus infections. These OsNAC genes might be related to the health stage maintenance of the host plants. Interestingly, most of the genes in the subgroups TIP and SNAC were more highly expressed during RBSDV and RGSV infections. These results suggested that OsNAC genes might be related to the responses induced by the virus infection. All of the genes assigned to the TIP subgroups were highly expressed during RGSV infection when compared with the control. For RDV infection, the number of activated genes was greatest during infection with the S-strain, followed by the D84-strain and the O-strain, with seven OsNAC genes up-regulated during infection by all three strains. The Os12g03050 and Os11g05614 genes showed higher expression during infection with four of the five viruses, and Os11g03310, Os11g03370, and Os07g37920 genes showed high expression during at least three viral infections. We identified some duplicate genes that are classified as neofunctional and subfunctional according to their expression levels in different viral infections. A number of putative cis-elements were identified, which may help to clarify the function of these key genes in network pathways. PMID:26442000

  8. Corn

    MedlinePlus

    ... composed of a dense core that presses on sensory nerves, causing extreme pain. Soft corns occur between ... a benign condition and may not require medical evaluation. However, if corns become very painful, evaluation should ...

  9. Dual transcriptome analysis reveals insights into the response to Rice black-streaked dwarf virus in maize.

    PubMed

    Zhou, Yu; Xu, Zhennan; Duan, Canxing; Chen, Yanping; Meng, Qingchang; Wu, Jirong; Hao, Zhuanfang; Wang, Zhenhua; Li, Mingshun; Yong, Hongjun; Zhang, Degui; Zhang, Shihuang; Weng, Jianfeng; Li, Xinhai

    2016-08-01

    Maize rough dwarf disease (MRDD) is a viral infection that results in heavy yield losses in maize worldwide, particularly in the summer maize-growing regions of China. MRDD is caused by the Rice black-streaked dwarf virus (RBSDV). In the present study, analyses of microRNAs (miRNAs), the degradome, and transcriptome sequences were used to elucidate the RBSDV-responsive pathway(s) in maize. Genomic analysis indicated that the expression of three non-conserved and 28 conserved miRNAs, representing 17 known miRNA families and 14 novel miRNAs, were significantly altered in response to RBSDV when maize was inoculated at the V3 (third leaf) stage. A total of 99 target transcripts from 48 genes of 10 known miRNAs were found to be responsive to RBSDV infection. The annotations of these target genes include a SQUAMOSA promoter binding (SPB) protein, a P450 reductase, an oxidoreductase, and a ubiquitin-related gene, among others. Characterization of the entire transcriptome suggested that a total of 28 and 1085 differentially expressed genes (DEGs) were detected at 1.5 and 3.0 d, respectively, after artificial inoculation with RBSDV. The expression patterns of cell wall- and chloroplast-related genes, and disease resistance- and stress-related genes changed significantly in response to RBSDV infection. The negatively regulated genes GRMZM2G069316 and GRMZM2G031169, which are the target genes for miR169i-p5 and miR8155, were identified as a nucleolin and a NAD(P)-binding Rossmann-fold superfamily protein in maize, respectively. The gene ontology term GO:0003824, including GRMZM2G031169 and other 51 DEGs, was designated as responsive to RBSDV. PMID:27493226

  10. Dual transcriptome analysis reveals insights into the response to Rice black-streaked dwarf virus in maize

    PubMed Central

    Zhou, Yu; Xu, Zhennan; Duan, Canxing; Chen, Yanping; Meng, Qingchang; Wu, Jirong; Hao, Zhuanfang; Wang, Zhenhua; Li, Mingshun; Yong, Hongjun; Zhang, Degui; Zhang, Shihuang; Weng, Jianfeng; Li, Xinhai

    2016-01-01

    Maize rough dwarf disease (MRDD) is a viral infection that results in heavy yield losses in maize worldwide, particularly in the summer maize-growing regions of China. MRDD is caused by the Rice black-streaked dwarf virus (RBSDV). In the present study, analyses of microRNAs (miRNAs), the degradome, and transcriptome sequences were used to elucidate the RBSDV-responsive pathway(s) in maize. Genomic analysis indicated that the expression of three non-conserved and 28 conserved miRNAs, representing 17 known miRNA families and 14 novel miRNAs, were significantly altered in response to RBSDV when maize was inoculated at the V3 (third leaf) stage. A total of 99 target transcripts from 48 genes of 10 known miRNAs were found to be responsive to RBSDV infection. The annotations of these target genes include a SQUAMOSA promoter binding (SPB) protein, a P450 reductase, an oxidoreductase, and a ubiquitin-related gene, among others. Characterization of the entire transcriptome suggested that a total of 28 and 1085 differentially expressed genes (DEGs) were detected at 1.5 and 3.0 d, respectively, after artificial inoculation with RBSDV. The expression patterns of cell wall- and chloroplast-related genes, and disease resistance- and stress-related genes changed significantly in response to RBSDV infection. The negatively regulated genes GRMZM2G069316 and GRMZM2G031169, which are the target genes for miR169i-p5 and miR8155, were identified as a nucleolin and a NAD(P)-binding Rossmann-fold superfamily protein in maize, respectively. The gene ontology term GO:0003824, including GRMZM2G031169 and other 51 DEGs, was designated as responsive to RBSDV. PMID:27493226

  11. Reconstructing the History of Maize Streak Virus Strain A Dispersal To Reveal Diversification Hot Spots and Its Origin in Southern Africa ▿ †

    PubMed Central

    Monjane, Adérito L.; Harkins, Gordon W.; Martin, Darren P.; Lemey, Philippe; Lefeuvre, Pierre; Shepherd, Dionne N.; Oluwafemi, Sunday; Simuyandi, Michelo; Zinga, Innocent; Komba, Ephrem K.; Lakoutene, Didier P.; Mandakombo, Noella; Mboukoulida, Joseph; Semballa, Silla; Tagne, Appolinaire; Tiendrébéogo, Fidèle; Erdmann, Julia B.; van Antwerpen, Tania; Owor, Betty E.; Flett, Bradley; Ramusi, Moses; Windram, Oliver P.; Syed, Rizwan; Lett, Jean-Michel; Briddon, Rob W.; Markham, Peter G.; Rybicki, Edward P.; Varsani, Arvind

    2011-01-01

    Maize streak virus strain A (MSV-A), the causal agent of maize streak disease, is today one of the most serious biotic threats to African food security. Determining where MSV-A originated and how it spread transcontinentally could yield valuable insights into its historical emergence as a crop pathogen. Similarly, determining where the major extant MSV-A lineages arose could identify geographical hot spots of MSV evolution. Here, we use model-based phylogeographic analyses of 353 fully sequenced MSV-A isolates to reconstruct a plausible history of MSV-A movements over the past 150 years. We show that since the probable emergence of MSV-A in southern Africa around 1863, the virus spread transcontinentally at an average rate of 32.5 km/year (95% highest probability density interval, 15.6 to 51.6 km/year). Using distinctive patterns of nucleotide variation caused by 20 unique intra-MSV-A recombination events, we tentatively classified the MSV-A isolates into 24 easily discernible lineages. Despite many of these lineages displaying distinct geographical distributions, it is apparent that almost all have emerged within the past 4 decades from either southern or east-central Africa. Collectively, our results suggest that regular analysis of MSV-A genomes within these diversification hot spots could be used to monitor the emergence of future MSV-A lineages that could affect maize cultivation in Africa. PMID:21715477

  12. Micropropagation by tissue culture triggers differential expression of infectious endogenous Banana streak virus sequences (eBSV) present in the B genome of natural and synthetic interspecific banana plantains.

    PubMed

    Côte, François X; Galzi, Serge; Folliot, Michel; Lamagnère, Yannick; Teycheney, Pierre-Yves; Iskra-Caruana, Marie-Line

    2010-01-01

    The genome of Musa balbisiana spp. contains several infectious endogenous sequences of Banana streak virus (eBSV). We have shown previously that in vitro micropropagation triggers the activation of infectious eBSOLV (endogenous sequences of Banana streak Obino l'Ewai virus) in the synthetic tetraploid interspecific hybrid FHIA21 (AAAB). In this work, we show that another synthetic tetraploid (AAAB) hybrid and two natural triploid (AAB) plantains are equally prone to the activation of infectious eBSOLV during tissue culture. These results are a strong indication that such activation is a general phenomenon in interspecific Musa cultivars, whether synthetic or natural. We also report the first in-depth study of the correlation between the duration of tissue culture and the level of activation of infectious eBSOLV, and show that specific and common activation patterns exist in these banana plants. We hypothesize that these patterns result from the concomitant activation of infectious eBSOLV and a decrease in the virus titre in neoformed plantlets, resulting from cell multiplication outcompeting virus replication. We provide experimental data supporting this hypothesis. No activation of infectious eBSGFV (endogenous sequences of Banana streak Goldfinger virus) by tissue culture was observed in the two natural AAB plantain cultivars studied here, whereas such activation occurred in the AAAB synthetic hybrid studied. We demonstrate that this differential activation does not result from differences in the structure of eBSGFV, as all banana genomes harbour eaBSGFV-7. PMID:20078782

  13. Transcriptome and Comparative Gene Expression Analysis of Sogatella furcifera (Horváth) in Response to Southern Rice Black-Streaked Dwarf Virus

    PubMed Central

    Xu, Yi; Zhou, Wenwu; Zhou, Yijun; Wu, Jianxiang; Zhou, Xueping

    2012-01-01

    Background The white backed planthopper (WBPH), Sogatella furcifera (Horváth), causes great damage to many crops by direct feeding or transmitting plant viruses. Southern rice black-streaked dwarf virus (SRBSDV), transmitted by WBPH, has become a great threat to rice production in East Asia. Methodology/Principal Findings By de novo transcriptome assembling and massive parallel pyrosequencing, we constructed two transcriptomes of WBPH and profiled the alternation of gene expression in response to SRBSDV infection in transcriptional level. Over 25 million reads of high-quality DNA sequences and 81388 different unigenes were generated using Illumina technology from both viruliferous and non-viruliferous WBPH. WBPH has a very similar gene ontological distribution to other two closely related rice planthoppers, Nilaparvata lugens and Laodelphax striatellus. 7291 microsatellite loci were also predicted which could be useful for further evolutionary analysis. Furthermore, comparative analysis of the two transcriptomes generated from viruliferous and non-viruliferous WBPH provided a list of candidate transcripts that potentially were elicited as a response to viral infection. Pathway analyses of a subset of these transcripts indicated that SRBSDV infection may perturb primary metabolism and the ubiquitin-proteasome pathways. In addition, 5.5% (181 out of 3315) of the genes in cell cytoskeleton organization pathway showed obvious changes. Our data also demonstrated that SRBSDV infection activated the immunity regulatory systems of WBPH, such as RNA interference, autophagy and antimicrobial peptide production. Conclusions/Significance We employed massively parallel pyrosequencing to collect ESTs from viruliferous and non-viruliferous samples of WBPH. 81388 different unigenes have been obtained. We for the first time described the direct effects of a Reoviridae family plant virus on global gene expression profiles of its insect vector using high-throughput sequencing. Our

  14. Identification and profiling of conserved and novel microRNAs in Laodelphax striatellus in response to rice black-streaked dwarf virus (RBSDV) infection

    PubMed Central

    Li, Jun-Min; Zhou, Yan-Ru; Sun, Zong-Tao; Wang, Xu; Xie, Li; Chen, Jian-Ping

    2014-01-01

    MicroRNAs (miRNAs) are small non-coding endogenous RNA molecules that play important roles in various biological processes. This study examined microRNA profiles of Laodelphax striatellus using the small RNA libraries derived from virus free (VF) and rice black-streaked dwarf virus (RBSDV) infected (RB) insects. A total of 59 mature miRNAs (46 miRNA families) were identified as conserved insect miRNAs in both VF and RB libraries. Among these conserved miRNAs, 24 were derived from the two arms of 12 miRNA precursors. Nine conserved L. striatellus miRNAs were up-regulated and 12 were down-regulated in response to RBSDV infection. In addition, a total of 20 potential novel miRNA candidates were predicted in the VF and RB libraries. The miRNA transcriptome profiles and the identification of L. striatellus miRNAs differentially expressed in response to RBSDV infection will contribute to future studies to elucidate the complex miRNA-mediated regulatory network activated by pathogen challenge in insect vectors. PMID:26484150

  15. Southern rice black-streaked dwarf virus (SRBSDV) directly affects the feeding and reproduction behavior of its vector, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

    PubMed Central

    2014-01-01

    Background Southern rice black-streaked dwarf virus (SRBSDV) is a recently discovered member of the genus Fijivirus and it is transmitted by the rice whitebacked planthopper (WBPH), Sogatella furcifera (Horváth). It was found that SRBSDV infected vectors might contribute negatively to the WBPH population, although the longer nymphal period might benefit viral acquisition, transmission and increase infection rate. The interaction between SRBSDV and its vector need to be further explored to gain better understanding of the dispersal of WBPH and the spread of virus disease, in particular the feeding and reproduction behavior of viruliferous WBPH. Methods Newly hatched nymphs of WBPH were fed on healthy rice plant after feeding on SRBSDV-infected rice plants for 2 h, and newly emerged adults were numbered and tested. Feeding behaviors of WBPH adults were monitored electronically within a Faraday cage using a Giga-4 DC EPG amplifier. The newly emerged adults were paired, and the fecundity and egg hatchability were investigated. WBPH was molecularly identified for SRBSDV when they dead. According to the identification results, data on viruliferous and non-viruliferous WBPH were collected and analyzed. Results Feeding behavior of viruliferous WBPH was different from those of non-viruliferous WBPH. Frequency of phloem sap ingestion of viruliferous WBPH increased significantly, however the total feeding duration did not increase markedly. When both WBPH parents were infected with SRBSDV, their fecundity and hatchability of the eggs produced were significant lower than those of normal WBPH parents. However, if only one of the parents was viruliferous, fecundity and egg hatchability were only slightly affected. Conclusions Viruliferous WBPH fed on the phloem more frequently than non-viruliferous WBPH and can thus contribute to virus transmission. When both vector parents are viruliferous fecundity and hatchability of the eggs were significantly reduced. However when only

  16. Interaction Research on the Antiviral Molecule Dufulin Targeting on Southern Rice Black Streaked Dwarf Virus P9-1 Nonstructural Protein

    PubMed Central

    Wang, Zhenchao; Li, Xiangyang; Wang, Wenli; Zhang, Weiying; Yu, Lu; Hu, Deyu; Song, Baoan

    2015-01-01

    Southern rice black streaked dwarf virus (SRBSDV) causes severe harm to rice production. Unfortunately, studies on effective antiviral drugs against SRBSDV and interaction mechanism of antiviral molecule targeting on SRBSDV have not been reported. This study found dufulin (DFL), an ideal anti-SRBSDV molecule, and investigated the interactions of DFL targeting on the nonstructural protein P9-1. The biological sequence information and bonding characterization of DFL to four kinds of P9-1 protein were described with fluorescence titration (FT) and microscale thermophoresis (MST) assays. The sequence analysis indicated that P9-1 had highly-conserved C- and N-terminal amino acid residues and a hypervariable region that differed from 131 aa to 160 aa. Consequently, wild-type (WT-His-P9-1), 23 C-terminal residues truncated (TR-ΔC23-His-P9-1), 6 N-terminal residues truncated (TR-ΔN6-His-P9-1), and Ser138 site-directed (MU-138-His-P9-1) mutant proteins were expressed. The FT and MST assay results indicated that DFL bounded to WT-His-P9-1 with micromole affinity and the 23 C-terminal amino acids were the potential targeting site. This system, which combines a complete sequence analysis, mutant protein expression, and binding action evaluating system, could further advance the understanding of the interaction abilities between antiviral drugs and their targets. PMID:25807053

  17. Population structure within lineages of Wheat streak mosaic virus derived from a common founding event exhibits stochastic variation inconsistent with the deterministic quasi-species model

    SciTech Connect

    French, Roy; Stenger, Drake C. . E-mail: dstenger@unlnotes.unl.edu

    2005-12-20

    Structure of Wheat streak mosaic virus (WSMV) populations derived from a common founding event and subjected to serial passage at high multiplicity of infection (MOI) was evaluated. The founding population was generated by limiting dilution inoculation. Lineages of known pedigree were sampled at passage 9 (two populations) and at passage 15, with (three populations) or without mixing (four populations) of lineages at passage 10. Polymorphism within each population was assessed by sequencing 17-21 clones containing a 1371 nt region (WSMV-Sidney 81 nts 8001-9371) encompassing the entire coat protein cistron and flanking regions. Mutation frequency averaged {approx}5.0 x 10{sup -4}/nt across all populations and ranged from 2.4 to 11.6 x 10{sup -4}/nt within populations, but did not consistently increase or decrease with the number of passages removed from the founding population. Shared substitutions (19 nonsynonymous, 10 synonymous, and 3 noncoding) occurred at 32 sites among 44 haplotypes. Only four substitutions became fixed (frequency = 100%) within a population and nearly one third (10/32) never achieved a frequency of 10% or greater in any sampled population. Shared substitutions were randomly distributed with respect to genome position, with transitions outnumbering transversions 5.4:1 and a clear bias for A to G and U to C substitutions. Haplotype composition of each population was unique with complexity of each population varying unpredictably, in that the number and frequency of haplotypes within a lineage were not correlated with number of passages removed from the founding population or whether the population was derived from a single or mixed lineage. The simplest explanation is that plant virus lineages, even those propagated at high MOI, are subject to frequent, narrow genetic bottlenecks during systemic movement that result in low effective population size and stochastic changes in population structure upon serial passage.

  18. Heritable, de novo resistance to leaf rust and other novel traits in selfed descendants of wheat responding to inoculation with wheat streak mosaic virus.

    PubMed

    Seifers, Dallas L; Haber, Steve; Martin, Terry J; McCallum, Brent D

    2014-01-01

    Stable resistance to infection with Wheat streak mosaic virus (WSMV) can be evolved de novo in selfing bread wheat lines subjected to cycles of WSMV inoculation and selection of best-performing plants or tillers. To learn whether this phenomenon might be applied to evolve resistance de novo to pathogens unrelated to WSMV, we examined the responses to leaf rust of succeeding generations of the rust- and WSMV-susceptible cultivar 'Lakin' following WSMV inoculation and derived rust-resistant sublines. After three cycles of the iterative protocol five plants, in contrast to all others, expressed resistance to leaf and stripe rust. A subset of descendant sublines of one of these, 'R1', heritably and uniformly expressed the new trait of resistance to leaf rust. Such sublines, into which no genes from a known source of resistance had been introgressed, conferred resistance to progeny of crosses with susceptible parents. The F1 populations produced from crosses between, respectively, susceptible and resistant 'Lakin' sublines 4-3-3 and 4-12-3 were not all uniform in their response to seedling inoculation with race TDBG. In seedling tests against TDBG and MKPS races the F2s from F1 populations that were uniformly resistant had 3∶1 ratios of resistant to susceptible individuals but the F2s from susceptible F1 progenitors were uniformly susceptible. True-breeding lines derived from resistant individuals in F2 populations were resistant to natural stripe and leaf rust inoculum in the field, while the 'Lakin' progenitor was susceptible. The next generation of six of the 'Lakin'-derived lines exhibited moderate to strong de novo resistance to stem rust races TPMK, QFCS and RKQQ in seedling tests while the 'Lakin' progenitor was susceptible. These apparently epigenetic effects in response to virus infection may help researchers fashion a new tool that expands the range of genetic resources already available in adapted germplasm. PMID:24497941

  19. Microchannel plate streak camera

    DOEpatents

    Wang, Ching L.

    1989-01-01

    An improved streak camera in which a microchannel plate electron multiplier is used in place of or in combination with the photocathode used in prior streak cameras. The improved streak camera is far more sensitive to photons (UV to gamma-rays) than the conventional x-ray streak camera which uses a photocathode. The improved streak camera offers gamma-ray detection with high temporal resolution. It also offers low-energy x-ray detection without attenuation inside the cathode. Using the microchannel plate in the improved camera has resulted in a time resolution of about 150 ps, and has provided a sensitivity sufficient for 1000 KeV x-rays.

  20. Microchannel plate streak camera

    DOEpatents

    Wang, C.L.

    1989-03-21

    An improved streak camera in which a microchannel plate electron multiplier is used in place of or in combination with the photocathode used in prior streak cameras is disclosed. The improved streak camera is far more sensitive to photons (UV to gamma-rays) than the conventional x-ray streak camera which uses a photocathode. The improved streak camera offers gamma-ray detection with high temporal resolution. It also offers low-energy x-ray detection without attenuation inside the cathode. Using the microchannel plate in the improved camera has resulted in a time resolution of about 150 ps, and has provided a sensitivity sufficient for 1,000 KeV x-rays. 3 figs.

  1. Microchannel plate streak camera

    DOEpatents

    Wang, C.L.

    1984-09-28

    An improved streak camera in which a microchannel plate electron multiplier is used in place of or in combination with the photocathode used in prior streak cameras. The improved streak camera is far more sensitive to photons (uv to gamma-rays) than the conventional x-ray streak camera which uses a photocathode. The improved streak camera offers gamma-ray detection with high temporal resolution. It also offers low-energy x-ray detection without attenuation inside the cathode. Using the microchannel plate in the improved camera has resulted in a time resolution of about 150 ps, and has provided a sensitivity sufficient for 1000 keV x-rays.

  2. Behavioral responses of the leafhopper, Cicadulina storeyi China, a major vector of maize streak virus, to volatile cues from intact and leafhopper-damaged maize.

    PubMed

    Oluwafemi, Sunday; Bruce, Toby J A; Pickett, John A; Ton, Jurriaan; Birkett, Michael A

    2011-01-01

    The chemical ecology of the leafhopper, Cicadulina storeyi China (Homoptera: Cicadellidae), an important vector of Maize Streak Virus (MSV), was studied with a view to developing novel leafhopper control strategies in sub-Saharan Africa. Choice tests using a Y-tube olfactometer revealed that odors from uninfested maize seedlings (Zea mays cv. Delprim) were significantly more attractive to C. storeyi than odors from C. storeyi-infested seedlings. Headspace samples of volatile organic compounds (VOCs) collected from 10 to 12 day-old uninfested seedlings were more attractive than those collected from infested seedlings. While VOCs collected from uninfested maize seedlings were attractive, VOCs collected from C. storeyi-infested seedlings were significantly repellent. Analysis of the collected VOCs by gas chromatography (GC) and coupled GC-mass spectrometry (GC-MS) led to the identification of myrcene, linalool, (E)-2-decen-1-ol, and decanal from uninfested seedlings, and (Z)-3-hexenyl acetate, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), methyl salicylate, benzyl acetate, indole, geranyl acetate, (E)-caryophyllene, α-bergamotene, (E)-β-farnesene, β-sesquiphellandrene, and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) from infested seedlings. Of these, methyl salicylate, (E)-caryophyllene, (E)-β-farnesene, and TMTT were identified previously as volatile semiochemicals involved in plant defense against other sucking insect pests. When tested individually for behavioral activity, all compounds were repellent for C. storeyi. Moreover, when these induced VOCs were added to the blend of VOCs from uninfested maize seedlings, a shift from attraction to repellency was observed. Addition of methyl salicylate, (E)-β-farnesene, or TMTT resulted in a choice for the solvent control (i.e., repulsion), whereas addition of (E)-caryophyllene resulted in no reduction in host VOC attractiveness. These results show that VOCs induced in maize have the potential to be exploited

  3. Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines.

    PubMed

    Nair, Sudha K; Babu, Raman; Magorokosho, Cosmos; Mahuku, George; Semagn, Kassa; Beyene, Yoseph; Das, Biswanath; Makumbi, Dan; Lava Kumar, P; Olsen, Michael; Boddupalli, Prasanna M

    2015-09-01

    Msv1 , the major QTL for MSV resistance was delimited to an interval of 0.87 cM on chromosome 1 at 87 Mb and production markers with high prediction accuracy were developed. Maize streak virus (MSV) disease is a devastating disease in the Sub-Saharan Africa (SSA), which causes significant yield loss in maize. Resistance to MSV has previously been mapped to a major QTL (Msv1) on chromosome 1 that is germplasm and environment independent and to several minor loci elsewhere in the genome. In this study, Msv1 was fine-mapped through QTL isogenic recombinant strategy using a large F 2 population of CML206 × CML312 to an interval of 0.87 cM on chromosome 1. Genome-wide association study was conducted in the DTMA (Drought Tolerant Maize for Africa)-Association mapping panel with 278 tropical/sub-tropical breeding lines from CIMMYT using the high-density genotyping-by-sequencing (GBS) markers. This study identified 19 SNPs in the region between 82 and 93 Mb on chromosome 1(B73 RefGen_V2) at a P < 1.00E-04, which coincided with the fine-mapped region of Msv1. Haplotype trend regression identified a haplotype block significantly associated with response to MSV. Three SNPs in this haplotype block at 87 Mb on chromosome 1 had an accuracy of 0.94 in predicting the disease reaction in a collection of breeding lines with known responses to MSV infection. In two biparental populations, selection for resistant Msv1 haplotype demonstrated a reduction of 1.03-1.39 units on a rating scale of 1-5, compared to the susceptible haplotype. High-throughput KASP assays have been developed for these three SNPs to enable routine marker screening in the breeding pipeline for MSV resistance. PMID:26081946

  4. Heritable, De Novo Resistance to Leaf Rust and Other Novel Traits in Selfed Descendants of Wheat Responding to Inoculation with Wheat Streak Mosaic Virus

    PubMed Central

    Seifers, Dallas L.; Haber, Steve; Martin, Terry J.; McCallum, Brent D.

    2014-01-01

    Stable resistance to infection with Wheat streak mosaic virus (WSMV) can be evolved de novo in selfing bread wheat lines subjected to cycles of WSMV inoculation and selection of best-performing plants or tillers. To learn whether this phenomenon might be applied to evolve resistance de novo to pathogens unrelated to WSMV, we examined the responses to leaf rust of succeeding generations of the rust- and WSMV-susceptible cultivar ‘Lakin’ following WSMV inoculation and derived rust-resistant sublines. After three cycles of the iterative protocol five plants, in contrast to all others, expressed resistance to leaf and stripe rust. A subset of descendant sublines of one of these, ‘R1’, heritably and uniformly expressed the new trait of resistance to leaf rust. Such sublines, into which no genes from a known source of resistance had been introgressed, conferred resistance to progeny of crosses with susceptible parents. The F1 populations produced from crosses between, respectively, susceptible and resistant ‘Lakin’ sublines 4-3-3 and 4-12-3 were not all uniform in their response to seedling inoculation with race TDBG. In seedling tests against TDBG and MKPS races the F2s from F1 populations that were uniformly resistant had 3∶1 ratios of resistant to susceptible individuals but the F2s from susceptible F1 progenitors were uniformly susceptible. True-breeding lines derived from resistant individuals in F2 populations were resistant to natural stripe and leaf rust inoculum in the field, while the ‘Lakin’ progenitor was susceptible. The next generation of six of the ‘Lakin’-derived lines exhibited moderate to strong de novo resistance to stem rust races TPMK, QFCS and RKQQ in seedling tests while the ‘Lakin’ progenitor was susceptible. These apparently epigenetic effects in response to virus infection may help researchers fashion a new tool that expands the range of genetic resources already available in adapted germplasm. PMID:24497941

  5. Crater and Wind Streak

    NASA Technical Reports Server (NTRS)

    2003-01-01

    MGS MOC Release No. MOC2-461, 23 August 2003

    This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a crater with a bright wind streak in southern Acidalia Planitia. The streak is mostly likely a very thin coating of dust. The orientation of the streak indicates that the winds responsible for its formation and maintenance came from the northeast (upper right) and blew toward the lower left (southwest). The crater is located near 24.8oN, 39.1oW. The image covers an area 3 km (1.9 mi) wide. Sunlight illuminates the scene from the lower left.

  6. Crater with Streak

    NASA Technical Reports Server (NTRS)

    2005-01-01

    20 June 2005 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a crater in the Memnonia region of Mars, around which has formed a wind streak. The bright streak is in the lee of the crater -- that is, it is on the crater's down-wind side. Thus, the winds responsible for the streak blew from the southeast (lower right).

    Location near: 6.7oS, 141.4oW Image width: 3 km (1.9 mi) Illumination from: upper left Season: Southern Spring

  7. STREAK damping. Technical report

    SciTech Connect

    Baker, J.; Peyton, S.; Freiberg, H.

    1989-12-01

    This report documents a study aimed at improving the damping in STREAK. A form and value for an artificial viscosity is recommended which appears to control ringing and overshoots without overdamping.

  8. Wind Streak Changes

    NASA Technical Reports Server (NTRS)

    2004-01-01

    2 September 2004 This pair of Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) images shows changes in dark wind streak patterns that occurred between 5 April 1999 (image M00-00534) and 17 August 2004 (image R20-00901). Unlike the spaghetti-like streak patterns made by dust devils, these streaks all begin on their upwind ends as tapered forms that fan outward in the downwind direction, and they all indicate winds that blew from the same direction. In both cases, winds blew from the southeast (lower right) toward the northwest (upper left). These streaks and the small pedestal craters found among them occur in the Memnonia region of Mars near 5.9oS, 162.2oW. The 400 meter scale bar is about 437 yards long. Sunlight illuminates each scene from the upper left.

  9. Daedalia Wind Streak

    NASA Technical Reports Server (NTRS)

    2004-01-01

    21 January 2004 This January 2004 Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a dark wind streak in the lee of a small meteor impact crater in Daedalia Planum. The dominant winds responsible for this streak blew from the east (right). This picture is located near 17.1oS, 138.8oW, and covers an area 3 km (1.9 mi) wide. Sunlight illuminates the scene from the left.

  10. Wind Streak and Crater

    NASA Technical Reports Server (NTRS)

    2004-01-01

    23 February 2004 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a wind streak developed in the lee of a meteor impact crater in western Daedalia Planum. The dominant winds responsible for the streak blew from the bottom/lower right (southeast). The image is located near 9.9oS, 144.9oW. Sunlight illuminates the scene from the lower left; the picture covers an area 3 km (1.9 mi) wide.

  11. Frosty Wind Streaks

    NASA Technical Reports Server (NTRS)

    2003-01-01

    MGS MOC Release No. MOC2-532, 2 November 2003

    As seasonal polar frosts sublime away each spring, winds may re-distribute some of the frost or move sediment exposed from beneath the frost. This action creates ephemeral wind streaks that can be used by scientists seeking to study the local circulation of the martian [missing text] surveyor (MGS) Mars Orbiter Camera (MOC) image shows a suite of wind streaks created in subliming carbon dioxide frost. These dark streaks appear to conform to the shape of the slopes on which they occur, suggesting that slope winds play a dominant role in creating and orienting these streaks. This picture is located near 73.8oS, 305.7oW. The image is illuminated by sunlight from the upper left and covers an area 3 km (1.9 mi) wide. Winds responsible for the streaks generally blew from the bottom/right (south/southeast) toward the top/upper left (north/northwest).

  12. Huygens Wind Streak

    NASA Technical Reports Server (NTRS)

    2005-01-01

    19 September 2005 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows the results of wind action on the floor of the giant martian impact basin, Huygens. The large crater in this image has a wind streak on its lee side, pointing toward the lower right (southeast). Usually, a light-toned wind streak behind a crater on Mars will be composed of a thin veneer of dust that the wind was not able to erode because it was protected by the presence of the crater's raised rims. In this case, the streak is caused by something different -- by the fact that dark, windblown sand has not been able to accumulate behind the crater.

    Location near: 13.0oS, 303.7oW Image width: width: 3 km (1.9 mi) Illumination from: lower left Season: Southern Spring

  13. Elysium Mons Wind Streak

    NASA Technical Reports Server (NTRS)

    2003-01-01

    MGS MOC Release No. MOC2-556, 26 November 2003

    This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a wind streak formed behind a meteor impact crater on the lower north flank of the volcano, Elysium Mons. Winds blow down the volcano slope, toward the northeast (toward upper right), causing a tail of uneroded dust to be captured behind the crater. Thin, filamentary dark streaks (resembling pencil scratches in this image) can be seen on the surface of the bright wind streak; these may have formed by disruption of surface dust by passing dust devils. This picture is located near 27.7oN, 212.7oW. The image covers an area 3 km (1.9 mi) wide, and is illuminated by sunlight from the left.

  14. Polar Dust Devil Streaks

    NASA Technical Reports Server (NTRS)

    2004-01-01

    30 June 2004 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image of dunes in the martian north polar region is important because it shows one of the highest northern latitude views of streaks thought to be made by passing dust devils. The dark, thin, filamentary streaks on the dunes and on the adjacent plains were probably formed by dust devils. The dunes occur near 76.6oN, 62.7oW. Dust devil streaks are observed on Mars at very high latitudes, such as this, all the way down to the equator. They are also seen at all elevations, from the deepest parts of the Hellas Basin to the summit of Olympus Mons. This picture covers an area about 3 km (1.9 mi) wide. Sunlight illuminates the scene from the lower left.

  15. Wind Streaks Among Flows

    NASA Technical Reports Server (NTRS)

    2004-01-01

    17 December 2004 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows dark wind streaks formed by removal of a thin veneer of bright dust covering small craters and lava flow surfacesnorthwest of Olympus Mons near 28.4oN, 129.8oW. Streak orientations indicate that the responsible winds blew from the east/southeast (right/lower right) toward the west/northwest (left/upper left). The image covers an area about 3 km (1.9 mi) wide. Sunlight illuminates thescene from the lower left.

  16. Devil-Streaked Plain

    NASA Technical Reports Server (NTRS)

    2006-01-01

    19 February 2006 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows dark streaks on a plain south of the giant impact basin, Hellas Planitia. The streaks map the routes traveled by dozens of individual southern spring and early summer dust devils.

    Location near: 68.4oS, 296.1oW Image width: 3 km (1.9 mi) Illumination from: upper left Season: Southern Summer

  17. Streak camera receiver definition study

    NASA Technical Reports Server (NTRS)

    Johnson, C. B.; Hunkler, L. T., Sr.; Letzring, S. A.; Jaanimagi, P.

    1990-01-01

    Detailed streak camera definition studies were made as a first step toward full flight qualification of a dual channel picosecond resolution streak camera receiver for the Geoscience Laser Altimeter and Ranging System (GLRS). The streak camera receiver requirements are discussed as they pertain specifically to the GLRS system, and estimates of the characteristics of the streak camera are given, based upon existing and near-term technological capabilities. Important problem areas are highlighted, and possible corresponding solutions are discussed.

  18. Craters and Streaks

    NASA Technical Reports Server (NTRS)

    2006-01-01

    1 April 2006 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows two impact craters of nearly equal size, plus their associated wind streaks. These occur in far eastern Chryse Planitia. The wind streaks point toward the southwest (lower left), indicating that the responsible winds blew from the northeast. One of the two craters is shallower than the other, and has a suite of large, windblown ripples on its floor. The shallower crater with the ripples is probably older than the other, deeper crater.

    Location near: 20.6oN, 30.1oW Image width: 3 km (1.9 mi) Illumination from: lower left Season: Northern Winter

  19. Dust Devil Streaks

    NASA Technical Reports Server (NTRS)

    2005-01-01

    27 November 2005 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a plethora of dark streaks created by spring and summer dust devil activity in Argyre Planitia.

    Location near: 64.9oS, 8.3oW Image width: width: 3 km (1.9 mi) Illumination from: upper left Season: Southern Summer

  20. Triton's streaks as windblown dust

    NASA Technical Reports Server (NTRS)

    Sagan, Carl; Chyba, Christopher

    1990-01-01

    Explanations for the surface streaks observed by Voyager 2 on Triton's southern hemisphere are discussed. It is shown that, despite Triton's tenuous atmosphere, low-cohesion dust trains with diameters of about 5 micron or less may be carried into suspension by aeolian surface shear stress, given expected geostrophic wind speeds of about 10 m/s. For geyser-like erupting dust plumes, it is shown that dust-settling time scales and expected wind velocities can produce streaks with length scales in good agreement with those of the streaks. Thus, both geyserlike eruptions or direct lifting by surface winds appear to be viable mechanisms for the origin of the streaks.

  1. Thrips-transmitted Viruses Infect a Number of Florida Crops

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The ilarviruses Tomato necrotic streak virus and Tobacco streak virus are present in south Florida. Both species cause economically significant disease in vegetable crop. Control of these viruses makes use of integrated pest management approaches....

  2. Daedalia Planum Wind Streak

    NASA Technical Reports Server (NTRS)

    2003-01-01

    MGS MOC Release No. MOC2-332, 16 April 2003

    This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows a complex streak formed by deposition and erosion of sediment by wind in the lee of an impact crater in western Daedalia Planum. The winds needed to create this feature blew from the southeast (from the lower right). The picture covers an area 3 km (1.9 mi) wide near 10.1oS, 133.7oW. Sunlight illuminates the scene from the upper left.

  3. Bright Devil Streaks

    NASA Technical Reports Server (NTRS)

    2005-01-01

    4 March 2005 In honor of Giovanni V. Schiaparelli's 170th birthday, we present this Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image showing light-toned dust devil streaks on the southern floor of Schiaparelli Crater.

    Location near: 5.3oS, 343.3oW Image width: 3 km (1.9 mi) Illumination from: upper left Season: Southern Autumn

  4. Bright Streak on Amalthea

    NASA Technical Reports Server (NTRS)

    2000-01-01

    These two images of Jupiter's small, irregularly shaped moon Amalthea, obtained by the camera onboard NASA's Galileo spacecraft in August 1999(left) and November 1999 (right), form a 'stereo pair' that helps scientists determine this moon's shape and the topography of its surface features. Features as small as 3.8 kilometers (2.4 miles) across can be resolved in these images, making them among the highest-resolution images ever taken of Amalthea.

    The large impact crater visible in both images, near the right-hand edge of Amalthea's disk, is about 40 kilometers (about 29 miles) across; two ridges, tall enough to cast shadows, extend from the top of the crater in a V-shape reminiscent of a 'rabbit ears' television antenna. To the left of these ridges, in the top center portion of Amalthea's disk, is a second large impact crater similar in size to the first crater. To the left of this second crater is a linear 'streak' of relatively bright material about 50 kilometers (31 miles) long. In previous spacecraft images of Amalthea taken from other viewing directions, this bright feature was thought to be a small, round, bright 'spot' and was given the name Ida. These new images reveal for the first time that Ida is actually a long, linear 'streak.' This bright streak may represent material ejected during the formation of the adjacent impact crater, or it may just mark the crest of a local ridge. Other patches of relatively bright material can be seen elsewhere on Amalthea's disk, although none of these other bright spots has Ida's linear shape.

    In both images, sunlight is coming from the left and north is approximately up. Note that the north pole of Amalthea is missing in the right-hand image (it was cut off by the edge of the camera frame). The bright streak, Ida, is on the side of the moon that faces permanently away from Jupiter, and the crater near the right-hand edge of the disk is in the center of Amalthea's leading side (the side of the moon that 'leads

  5. Streaking into Middle School Science: The Dell Streak Pilot Project

    ERIC Educational Resources Information Center

    Austin, Susan Eudy

    2012-01-01

    A case study is conducted implementing the Dell Streak seven-inch android device into eighth grade science classes of one teacher in a rural middle school in the Piedmont region of North Carolina. The purpose of the study is to determine if the use of the Dell Streaks would increase student achievement on standardized subject testing, if the…

  6. 9. VIEW OF 'BLUE STREAK' HAMMER MILL (Prater Pulverizer Co., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW OF 'BLUE STREAK' HAMMER MILL (Prater Pulverizer Co., Chicago, Illinois), LOCATED IN THE SOUTHEAST CORNER OF THE BASEMENT, WAS ADDED IN THE EARLY 1930s. THIS WAS THE MILL'S FIRST ELECTRIC-POWERED MACHINERY. THE HAMMER MILL WAS USED TO PULVERIZE OATS, ALFALFA MEAL, AND CORN. Photographer: Louise Taft Cawood, July 1986 - Alexander's Grist Mill, Lock 37 on Ohio & Erie Canal, South of Cleveland, Valley View, Cuyahoga County, OH

  7. Light and Dark Slope Streaks

    NASA Technical Reports Server (NTRS)

    2004-01-01

    21 July 2004 Dark slope streaks are a common feature on slopes thickly-mantled by dust, especially in the Tharsis, Arabia, and western Amazonis regions of Mars. Less common are light-toned slope streaks, which often occur in the same area as dark streaks. They are most common in Arabia Terra, and some are shown in this Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image. Slope streaks are probably the result of sudden avalanches of extremely dry dust. The behavior of the avalanching dust is somewhat fluid-like, and new streaks have been observed to form over intervals of a few months to a Mars year. This image is located near 13.4oN, 340.3oW, and covers an area about 3 km (1.9 mi) wide. Sunlight illuminates the scene from the lower left.

  8. Cerberus Wind Streaks

    NASA Technical Reports Server (NTRS)

    2002-01-01

    (Released 6 May 2002) The Science Cerberus is a dark region on Mars that has shrunk down from a continuous length of about 1000 km to roughly three discontinuous spots a few 100 kms in length in less than 20 years. There are two competing processes at work in the Cerberus region that produce the bright and dark features seen in this THEMIS image. Bright dust settles out of the atmosphere, especially after global dust storms, depositing a layer just thick enough to brighten the dark surfaces. Deposition occurs preferentially in the low wind 'shadow zones' within craters and downwind of crater rims, producing the bright streaks. The direction of the streaks clearly indicates that the dominant winds come from the northeast. Dust deposition would completely blot out the dark areas if it were not for the action of wind-blown sand grains scouring the surface and lifting the dust back into the atmosphere. Again, the shadow zones are protected from the blowing sand, preserving the bright layer of dust. Also visible in this image are lava flow features extending from the flanks of the huge Elysium volcanoes to the northwest. Two shallow channels and a raised flow lobe are just barely discernable. The lava channel in the middle of the image crosses the boundary of the bright and dark surfaces without any obvious change in its morphology. This demonstrates that the bright dust layer is very thin in this location, perhaps as little as a few millimeters. The Story Mars is an ever-changing land of spectacular contrasts. This THEMIS image shows the Cerberus region of Mars, a dark area located near the Elysium volcanoes and fittingly named after the three-headed, dragon-tailed dog who guards the door of the underworld. Two opposing processes are at work here: a thin layer of dust falling from the atmosphere and/or dust storms creating brighter surface areas (e.g. the top left portion of this image) and dust being scoured away by the action of the Martian wind disturbing the sand

  9. Wheat streak mosaic virus P1, not HC-Pro, facilitates disease synergism and suppression of post-transcriptional gene silencing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Potyvirus HC-Pro is a suppressor of post-transcriptional gene silencing (PTGS). One consequence of PTGS suppression is pathogenicity enhancement, resulting in disease synergism upon mixed infection of a potyvirus and an unrelated virus. Tritimoviruses also encode HC-Pro sharing limited sequence i...

  10. Comparison of Streak Tube Performance

    SciTech Connect

    Lerche, R A; Andrews, D S; Bell, P M; Griffiths, R L; Huey, A W; McDonald, J W; de Dios, G V

    2004-11-04

    The performance of four streak tubes in six streak camera configurations is reported. Evaluations were made as part of a search for a streak tube to replace the obsolete RCA C73435 used in the ICF Program's optical streak cameras. Characteristics measured include linearity, spatial and temporal resolution, line-spread function, contrast transfer ratio (CTR), and dynamic range. Tubes evaluated are the RCA C73435, Photonis P510, Photek ST-Y, and Hamamatsu N8059. The RCA C73435 was evaluated in three camera configurations: large format CCD coupled directly to the streak tube, CCD directly coupled to an image intensifier tube (IIT), and the original configuration with a smaller CCD lens coupled to the IIT output. The Photonis and Photek tubes were characterized in configurations where they were directly coupled to large format CCDs. Optimum spatial resolution is achieved when the IIT is removed. Maximum dynamic range requires a configuration where a single photoelectron from the photocathode produces a signal that is {approx}5 times the CCD read noise. The Photonis P510 tube with the E2V CCD forms a well-optimized streak camera system.

  11. Characterization of Dark Streaks on Martian Slopes

    NASA Astrophysics Data System (ADS)

    Fenton, L. K.; Danielson, G. E.; Albee, A.; MGS MOC Science Team

    1998-09-01

    The narrow angle MOC images from the Mars Global Surveyor (MGS) show dozens of examples of dark streaks on Martian hillsides that may be indicative of fluid flow. In many cases multiple streaks seem to initiate from a narrow layer near the tops of steep slopes. They appear from these layers as narrow features that quickly broaden and then either remain the same width downhill or narrow and disappear. The largest streak observed to date is approximately 2 km in length and 100 m in width. MOLA data is not yet available to determine the slope down the length of the streaks. The lack of variation in brightness downhill and across the streaks shows that the formation of each streak occurred quickly. The streaks are widely distributed globally, but seem to be confined to layered materials. The streaks' characteristics show not only that the material forming them moved rapidly downslope but also that the streak material originated within the nearby bedrock. Change in brightness from one streak to another provides relative streak ages, assuming that dust infall causes an increase in streak albedo. The dark streaks are caused by a process that appears similar to fluid flow at the resolution of the MOC images. Possible causes of the streaks are water flow, lava flow, and debris flow. Our results are not yet conclusive as to the origins of the dark streaks, but we narrow the range of possible formation processes.

  12. Streak camera dynamic range optimization

    SciTech Connect

    Wiedwald, J.D.; Lerche, R.A.

    1987-09-01

    The LLNL optical streak camera is used by the Laser Fusion Program in a wide range of applications. Many of these applications require a large recorded dynamic range. Recent work has focused on maximizing the dynamic range of the streak camera recording system. For our streak cameras, image intensifier saturation limits the upper end of the dynamic range. We have developed procedures to set the image intensifier gain such that the system dynamic range is maximized. Specifically, the gain is set such that a single streak tube photoelectron is recorded with an exposure of about five times the recording system noise. This ensures detection of single photoelectrons, while not consuming intensifier or recording system dynamic range through excessive intensifier gain. The optimum intensifier gain has been determined for two types of film and for a lens-coupled CCD camera. We have determined that by recording the streak camera image with a CCD camera, the system is shot-noise limited up to the onset of image intensifier nonlinearity. When recording on film, the film determines the noise at high exposure levels. There is discussion of the effects of slit width and image intensifier saturation on dynamic range. 8 refs.

  13. The Rochester Optical Streak System

    NASA Astrophysics Data System (ADS)

    Jaanimagi, P. A.; Boni, R.; Keck, R. L.; Donaldson, W. R.; Meyerhofer, D. D.

    2004-11-01

    A modern, self-calibrating, remotely controlled streak camera platform capable of accepting a variety of different streak tubes has been developed. Our current systems include P510, P820, and PJX streak tubes with both S-1 and S-20 photocathodes. The input may be either fiber delivery or free-space propagation and is relayed to the photocathode with an achromatic Offner triplet. Calibration functions include autofocus of the input and electron optics, geometric distortion and flat-field correction, system gain, and linearity. The four remotely selectable sweep speeds are calibrated with an on-board optical comb generator. The recording system is a fiber-coupled back-illuminated CCD camera. We will present data illustrating the many features of the ROSS camera as applied to laser and plasma diagnostics. This work was supported by the U.S. Department of Energy Office of Inertial Confinement Fusion under Cooperative Agreement No. DE-FC52-92SF19460.

  14. Circular Scan Streak Tube Development

    NASA Technical Reports Server (NTRS)

    Nevin, S.

    1980-01-01

    A streak tube having circular scan was designed, built and tested. Continuous circular scan, easily derived from out of phase sine waves applied to the conventional deflection plates, permits the timing of pulses traveling long baselines. At the tube's output a circular array of 720 elements is scanned to provide 30 to 40 picosecond resolution. Initial difficulties with electron bombarded silicon arrays were circumvented by using microchannel plates within the streak tube to provide the needed electronic amplification and digital sensitivity and coupling the 720 element arrays to the electron beam by means of a phosphor on a fiber optics. Two ceramic body tubes with S-20 photocathodes were tested and delivered.

  15. Streaking into middle school science: The Dell Streak pilot project

    NASA Astrophysics Data System (ADS)

    Austin, Susan Eudy

    A case study is conducted implementing the Dell Streak seven-inch android device into eighth grade science classes of one teacher in a rural middle school in the Piedmont region of North Carolina. The purpose of the study is to determine if the use of the Dell Streaks would increase student achievement on standardized subject testing, if the Streak could be used as an effective instructional tool, and if it could be considered an effective instructional resource for reviewing and preparing for the science assessments. A mixed method research design was used for the study to analyze both quantitative and qualitative results to determine if the Dell Streaks' utilization could achieve the following: 1. instructional strategies would change, 2. it would be an effective instructional tool, and 3. a comparison of the students' test scores and benchmark assessments' scores would provide statistically significant difference. Through the use of an ANOVA it was determined a statistically significant difference had occurred. A Post Hoc analysis was conducted to identify where the difference occurred. Finally a T-test determined was there was no statistically significance difference between the mean End-of-Grade tests and four quarterly benchmark scores of the control and the experimental groups. Qualitative research methods were used to gather results to determine if the Streaks were an effective instructional tool. Classroom observations identified that the teacher's teaching styles and new instructional strategies were implemented throughout the pilot project. Students had an opportunity to complete a questionnaire three times during the pilot project. Results revealed what the students liked about using the devices and the challenges they were facing. The teacher completed a reflective questionnaire throughout the pilot project and offered valuable reflections about the use of the devices in an educational setting. The reflection data supporting the case study was drawn

  16. Slope Streaks in Terra Sabaea

    NASA Technical Reports Server (NTRS)

    2007-01-01

    [figure removed for brevity, see original site] Figure 1 Click on image for larger version

    This HiRISE image shows the rim of a crater in the region of Terra Sabaea in the northern hemisphere of Mars.

    The subimage (figure 1) is a close-up view of the crater rim revealing dark and light-toned slope streaks. Slope streak formation is among the few known processes currently active on Mars. While their mechanism of formation and triggering is debated, they are most commonly believed to form by downslope movement of extremely dry sand or very fine-grained dust in an almost fluidlike manner (analogous to a terrestrial snow avalanche) exposing darker underlying material.

    Other ideas include the triggering of slope streak formation by possible concentrations of near-surface ice or scouring of the surface by running water from aquifers intercepting slope faces, spring discharge (perhaps brines), and/or hydrothermal activity.

    Several of the slope streaks in the subimage, particularly the three longest darker streaks, show evidence that downslope movement is being diverted around obstacles such as large boulders. Several streaks also appear to originate at boulders or clumps of rocky material.

    In general, the slope streaks do not have large deposits of displaced material at their downslope ends and do not run out onto the crater floor suggesting that they have little reserve kinetic energy. The darkest slope streaks are youngest and can be seen to cross cut and superpose older and lighter-toned streaks. The lighter-toned streaks are believed to be dark streaks that have lightened with time as new dust is deposited on their surface.

    Observation Geometry Image PSP_001808_1875 was taken by the High Resolution Imaging Science Experiment (HiRISE) camera onboard the Mars Reconnaissance Orbiter spacecraft on 15-Dec-2006. The complete image is centered at 7.4 degrees latitude, 47.0 degrees East longitude. The range to the target site was 272.1 km

  17. Dark streaks on talus slopes, Mars

    NASA Astrophysics Data System (ADS)

    Ferguson, H. M.; Lucchitta, B. K.

    1984-04-01

    High-resolution pictures of talus slopes on Mars show small, dark streaks that characteristically widen downward. These streaks are different from the thin and even streaks of various albedos that stream from cliffs on talus slopes, but gradations between the two streak types occur and not all streaks can be classified with confidence. In order to study the nature and origin of the small, widening, dark streaks, all Viking pictures with a resolution of less than 100 m/pixel were surveyed. To date several hundred streaks were located, but only few are of high enough resolution to be confidently identified as widening downwards. The approximate dimensions of the streaks were measured and their shapes, numbers, position, and spacing on slopes were noted. They were plotted on a topographic map, and their relation to topography, geologic units, and regions of distinct thermal inertia and albedo were studied. Also noted was the season at which images containing streaks were acquired and the direction of illumination. Albedo measurements are in progress. Several streaks can be seen stereoscopically, but none are observed on color images. The observation of small dark streaks on talus slopes on Mars is compatible with an interpretation of their origin as eruptions of small masses of wet debris in places where steep walls intersect aquifers or where seasonal equatorial warming permits the local melting of ground ice.

  18. Wind vs. Dust Devil Streaks

    NASA Technical Reports Server (NTRS)

    2004-01-01

    22 February 2004 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image presents a fine illustration of the difference between streaks made by dust devils and streaks made by wind gusts. Dust devils are usually solitary, spinning vortices. They resemble a tornado, or the swirling motion of a familiar, Tasmanian cartoon character. Wind gusts, on the other hand, can cover a larger area and affect more terrain at the same time. The dark, straight, and parallel features resembling scrape marks near the right/center of this image are thought to have been formed by a singular gust of wind, whereas the more haphazard dark streaks that crisscross the scene were formed by dozens of individual dust devils, acting at different times. This southern summer image is located in Noachis Terra near 67.0oS, 316.2oW. Sunlight illuminates the scene from the upper left; the picture covers an area 3 km (1.9 mi) wide.

  19. Raspberry (Rubus spp.)-Viruses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There are several important virus diseases of raspberry and black raspberry in the Pacific Northwest. Pollen-borne viruses include Raspberry bushy dwarf virus and Strawberry necrotic shock virus (aka Tobacco streak virus –Rubus isolate or Black raspberry latent virus). Strawberry necrotic shock viru...

  20. A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia.

    PubMed

    Hassani-Mehraban, Afshin; Botermans, Marleen; Verhoeven, J Th J; Meekes, Ellis; Saaijer, Janneke; Peters, Dick; Goldbach, Rob; Kormelink, Richard

    2010-03-01

    A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5' untranslated region and a part of the intergenic region 3' of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV. PMID:20151164

  1. Cassava brown streak disease: a threat to food security in Africa.

    PubMed

    Patil, Basavaprabhu L; Legg, James P; Kanju, Edward; Fauquet, Claude M

    2015-05-01

    Cassava brown streak disease (CBSD) has emerged as the most important viral disease of cassava (Manihot esculenta) in Africa and is a major threat to food security. CBSD is caused by two distinct species of ipomoviruses, Cassava brown streak virus and Ugandan cassava brown streak virus, belonging to the family Potyviridae. Previously, CBSD was reported only from the coastal lowlands of East Africa, but recently it has begun to spread as an epidemic throughout the Great Lakes region of East and Central Africa. This new spread represents a major threat to the cassava-growing regions of West Africa. CBSD-resistant cassava cultivars are being developed through breeding, and transgenic RNA interference-derived field resistance to CBSD has also been demonstrated. This review aims to provide a summary of the most important studies on the aetiology, epidemiology and control of CBSD and to highlight key research areas that need prioritization. PMID:26015320

  2. Black streak root rot of lentil

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Black streak root rot of lentil is caused by the soil borne fungus Thielaviopsis basicola. The pathogen is widespread. The disease shows symptoms of black streaking on root, and stunted plants. The disease is favored by cool and moist weather. Management of the disease rely on avoiding fields wi...

  3. Earth and planetary aeolian streaks: A review

    NASA Astrophysics Data System (ADS)

    Cohen-Zada, Aviv Lee; Blumberg, Dan Gabriel; Maman, Shimrit

    2016-03-01

    Wind streaks are abundant aeolian features that have been observed on planetary surfaces by remote sensing means. They have been widely studied, particularly on Mars and Venus and to a much lesser extent on Earth. In imagery, these streaks appear as elongated features that are easily distinguishable from their surroundings. Geomorphologically, these streaks have, thus far, been interpreted as the presence or absence of small loose particles on the surface, deposited or eroded, respectively, by wind. However, the use of different (optical and radar) remote-sensing tools to study wind streaks has led to uncertain interpretations of these features and has hindered their geomorphological definition. Since wind streaks indicate the prevailing wind direction at the time of their formation, they may be used to map near-surface winds and to estimate atmospheric circulation patterns. The aim of this article is to review the main studies focusing on wind streaks and to present the most up-to-date knowledge on this topic. Moreover, a new perspective for wind streak research is suggested: As 'wind streak' is a collective term for a variety of aeolian features that when viewed from above appear as distinctive albedo surface patterns, we suggest that the term should not be used to refer to a geomorphological feature. Since the definition of wind streaks is constrained to remote sensing rather than to geomorphology and is affected by the inherent biases of remote sensing methods, we suggest that 'wind streaks' should be used as a collective term for aeolian surfaces that are discernable from above as bright and dark patterns due to alterations in the characteristics of the surface or to the presence of bedforms. To better understand the mechanisms, time-frames, climate compatibility of wind streaks and the influences of remote sensing on their appearance, we have compiled a new database containing more than 2,900 Earth wind streaks. A comprehensive study of these Earth wind

  4. Wind Streaks on Earth; Exploration and Interpretation

    NASA Astrophysics Data System (ADS)

    Cohen-Zada, Aviv Lee; Blumberg, Dan G.; Maman, Shimrit

    2015-04-01

    Wind streaks, one of the most common aeolian features on planetary surfaces, are observable on the surface of the planets Earth, Mars and Venus. Due to their reflectance properties, wind streaks are distinguishable from their surroundings, and they have thus been widely studied by remote sensing since the early 1970s, particularly on Mars. In imagery, these streaks are interpreted as the presence - or lack thereof - of small loose particles on the surface deposited or eroded by wind. The existence of wind streaks serves as evidence for past or present active aeolian processes. Therefore, wind streaks are thought to represent integrative climate processes. As opposed to the comprehensive and global studies of wind streaks on Mars and Venus, wind streaks on Earth are understudied and poorly investigated, both geomorphologically and by remote sensing. The aim of this study is, thus, to fill the knowledge gap about the wind streaks on Earth by: generating a global map of Earth wind streaks from modern high-resolution remotely sensed imagery; incorporating the streaks in a geographic information system (GIS); and overlaying the GIS layers with boundary layer wind data from general circulation models (GCMs) and data from the ECMWF Reanalysis Interim project. The study defines wind streaks (and thereby distinguishes them from other aeolian features) based not only on their appearance in imagery but more importantly on their surface appearance. This effort is complemented by a focused field investigation to study wind streaks on the ground and from a variety of remotely sensed images (both optical and radar). In this way, we provide a better definition of the physical and geomorphic characteristics of wind streaks and acquire a deeper knowledge of terrestrial wind streaks as a means to better understand global and planetary climate and climate change. In a preliminary study, we detected and mapped over 2,900 wind streaks in the desert regions of Earth distributed in

  5. Orbital objects detection algorithm using faint streaks

    NASA Astrophysics Data System (ADS)

    Tagawa, Makoto; Yanagisawa, Toshifumi; Kurosaki, Hirohisa; Oda, Hiroshi; Hanada, Toshiya

    2016-02-01

    This study proposes an algorithm to detect orbital objects that are small or moving at high apparent velocities from optical images by utilizing their faint streaks. In the conventional object-detection algorithm, a high signal-to-noise-ratio (e.g., 3 or more) is required, whereas in our proposed algorithm, the signals are summed along the streak direction to improve object-detection sensitivity. Lower signal-to-noise ratio objects were detected by applying the algorithm to a time series of images. The algorithm comprises the following steps: (1) image skewing, (2) image compression along the vertical axis, (3) detection and determination of streak position, (4) searching for object candidates using the time-series streak-position data, and (5) selecting the candidate with the best linearity and reliability. Our algorithm's ability to detect streaks with signals weaker than the background noise was confirmed using images from the Australia Remote Observatory.

  6. Bright Streaks and Dark Fans

    NASA Technical Reports Server (NTRS)

    2007-01-01

    The south polar region of Mars is covered every year by a layer of carbon dioxide ice. In a region called the 'cryptic terrain,' the ice is translucent and sunlight can penetrate through the ice to warm the surface below.

    The ice layer sublimates (evaporates) from the bottom. The dark fans of dust seen in this image come from the surface below the layer of ice, carried to the top by gas venting from below. The translucent ice is 'visible' by virtue of the effect it has on the tone of the surface below, which would otherwise have the same color and reflectivity as the fans.

    Bright streaks in this image are fresh frost. The CRISM team has identified the composition of these streaks to be carbon dioxide.

    Observation Geometry Image PSP_003113_0940 was taken by the High Resolution Imaging Science Experiment (HiRISE) camera onboard the Mars Reconnaissance Orbiter spacecraft on 26-Mar-2007. The complete image is centered at -85.8 degrees latitude, 106.0 degrees East longitude. The range to the target site was 244.9 km (153.0 miles). At this distance the image scale is 49.0 cm/pixel (with 2 x 2 binning) so objects 147 cm across are resolved. The image shown here has been map-projected to 50 cm/pixel . The image was taken at a local Mars time of 06:20 PM and the scene is illuminated from the west with a solar incidence angle of 79 degrees, thus the sun was about 11 degrees above the horizon. At a solar longitude of 207.6 degrees, the season on Mars is Northern Autumn.

  7. Electro-optic Phase Grating Streak Spectrometer

    SciTech Connect

    Goldin, F. J.

    2012-08-02

    The electro-optic phase grating streak spectrometer (EOPGSS) generates a time-resolved spectra equivalent to that obtained with a conventional spectrometer/streak camera combination, but without using a streak camera (by far the more expensive and problematic component of the conventional system). The EOPGSS is based on a phase, rather than an amplitude grating. Further, this grating is fabricated of electro-optic material such as, for example, KD*P, by either etching grooves into an E-O slab, or by depositing lines of the E-O material onto an optical flat. An electric field normal to the grating alters the material’s index of refraction and thus affects a shift (in angle) of the output spectrum. Ramping the voltage streaks the spectrum correspondingly. The streak and dispersion directions are the same, so a second (static, conventional) grating disperses the spectrum in the orthogonal direction to prevent different wavelengths from “overwriting” each other. Because the streaking is done by the grating, the streaked output spectrum is recorded with a time-integrating device, such as a CCD. System model, typical design, and performance expectations will be presented.

  8. Compact Optical Technique for Streak Camera Calibration

    SciTech Connect

    Curt Allen; Terence Davies; Frans Janson; Ronald Justin; Bruce Marshall; Oliver Sweningsen; Perry Bell; Roger Griffith; Karla Hagans; Richard Lerche

    2004-04-01

    The National Ignition Facility is under construction at the Lawrence Livermore National Laboratory for the U.S. Department of Energy Stockpile Stewardship Program. Optical streak cameras are an integral part of the experimental diagnostics instrumentation. To accurately reduce data from the streak cameras a temporal calibration is required. This article describes a technique for generating trains of precisely timed short-duration optical pulses that are suitable for temporal calibrations.

  9. Performance comparison of streak camera recording systems

    SciTech Connect

    Derzon, M.; Barber, T.

    1995-07-01

    Streak camera based diagnostics are vital to the inertial confinement fusion program at Sandia National Laboratories. Performance characteristics of various readout systems coupled to an EGG-AVO streak camera were analyzed and compared to scaling estimates. The purpose of the work was to determine the limits of the streak camera performance and the optimal fielding conditions for the Amador Valley Operations (AVO) streak camera systems. The authors measured streak camera limitations in spatial resolution and sensitivity. Streak camera limits on spatial resolution are greater than 18 lp/mm at 4% contrast. However, it will be difficult to make use of any resolution greater than this because of high spatial frequency variation in the photocathode sensitivity. They have measured a signal to noise of 3,000 with 0.3 mW/cm{sup 2} of 830 nm light at a 10 ns/mm sweep speed. They have compared lens coupling systems with and without micro-channel plate intensifiers and systems using film or charge coupled device (CCD) readout. There were no conditions where film was found to be an improvement over the CCD readout. Systems utilizing a CCD readout without an intensifier have comparable resolution, for these source sizes and at a nominal cost in signal to noise of 3, over those with an intensifier. Estimates of the signal-to-noise for different light coupling methods show how performance can be improved.

  10. CORN FLAVOR

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn is a large part of the modern diet through sweeteners, oil, processed foods, and animal-derived foods. In addition, corn is eaten directly in bread and cereal-type foods, snack foods, and foods made from masa flour. Corn gluten meal is a byproduct of grain processed by wet milling. Although pri...

  11. Update on the Rochester Optical Streak System

    NASA Astrophysics Data System (ADS)

    Jaanimagi, P. A.

    2005-10-01

    The Rochester Optical Streak System (ROSS) is a modern, self-calibrating, remotely controlled streak camera platform capable of accepting a variety of different streak tubes. The optical calibration module (OCM) for the ROSS camera has been completed and integrated with the main streak tube housing. The OCM incorporates an achromatic Offner triplet that allows fiber-delivered input and free-space propagated signals to be simultaneously relayed to the photocathode. It also encloses of the light sources and reticles required to accomplish a full suite of system calibrations including: autofocus of the input and electron optics, geometric distortion and flat-field correction, time base, system gain, and linearity. We will present data illustrating the system capabilities and our latest results comparing the dynamic performance of the P510 and P820 streak tubes. This work was supported by the U.S. Department of Energy Office of Inertial Confinement Fusion under Cooperative Agreement No. DE-FC52-92SF19460.

  12. Hot streak characterization in serpentine exhaust nozzles

    NASA Astrophysics Data System (ADS)

    Crowe, Darrell S.

    Modern aircraft of the United States Air Force face increasingly demanding cost, weight, and survivability requirements. Serpentine exhaust nozzles within an embedded engine allow a weapon system to fulfill mission survivability requirements by providing denial of direct line-of-sight into the high-temperature components of the engine. Recently, aircraft have experienced material degradation and failure along the aft deck due to extreme thermal loading. Failure has occurred in specific regions along the aft deck where concentrations of hot gas have come in contact with the surface causing hot streaks. The prevention of these failures will be aided by the accurate prediction of hot streaks. Additionally, hot streak prediction will improve future designs by identifying areas of the nozzle and aft deck surfaces that require thermal management. To this end, the goal of this research is to observe and characterize the underlying flow physics of hot streak phenomena. The goal is accomplished by applying computational fluid dynamics to determine how hot streak phenomena is affected by changes in nozzle geometry. The present research first validates the computational methods using serpentine inlet experimental and computational studies. A design methodology is then established for creating six serpentine exhaust nozzles investigated in this research. A grid independent solution is obtained on a nozzle using several figures of merit and the grid-convergence index method. An investigation into the application of a second-order closure turbulence model is accomplished. Simulations are performed for all serpentine nozzles at two flow conditions. The research introduces a set of characterization and performance parameters based on the temperature distribution and flow conditions at the nozzle throat and exit. Examination of the temperature distribution on the upper and lower nozzle surfaces reveals critical information concerning changes in hot streak phenomena due to changes

  13. Frost streaks in the south polar cap of Mars

    NASA Technical Reports Server (NTRS)

    Thomas, P.; Veverka, J.; Campos-Marquetti, R.

    1979-01-01

    Viking Orbiter images of the annual south polar cap on Mars exhibit elongated bright features that are associated with craters and resemble wind streaks observed elsewhere on Mars. The study focuses on the well-documented frost streaks. The discussion covers the morphology of frost streaks, occurrence, seasonal behavior, thickness of frost in streak deposits, wind patterns inferred from frost streaks and other eolian features in the south polar region, formation of frost streaks, and other locales of preferential frost accumulation. The form and seasonal behavior of the bright elongated albedo markings which extend from the rims of many craters in the south polar cap suggest that they are accumulations of CO2 frost in the lee of craters. The frost streaks appear in the fall, increasing in length but not changing in direction during fall and winter. The frost streaks indicate a prograde circulation pattern of near-surface winds around the pole. Other details are also presented.

  14. Corn Insect Pests

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Historically, the major corn insect pests have been corn rootworms (northern and western), European corn borer, and black cutworm. Bt-corn hybrids are effective against most of these pests. However, Bt-corn hybrids are not effective against corn leaf aphid, corn root aphid, sap beetles, corn rootwor...

  15. ASTRiDE: Automated Streak Detection for Astronomical Images

    NASA Astrophysics Data System (ADS)

    Kim, Dae-Won

    2016-05-01

    ASTRiDE detects streaks in astronomical images using a "border" of each object (i.e. "boundary-tracing" or "contour-tracing") and their morphological parameters. Fast moving objects such as meteors, satellites, near-Earth objects (NEOs), or even cosmic rays can leave streak-like traces in the images; ASTRiDE can detect not only long streaks but also relatively short or curved streaks.

  16. Corn transformed

    SciTech Connect

    Moffat, A.S.

    1990-08-10

    Researchers have produced fertile corn transformed with a foreign gene that makes the plants resistant to the herbicide bialaphos. This achievement, is the first report of fertile transgenic corn in the reviewed literature, and it is the capstone of almost a decade's efforts to genetically engineer this country's most important crop. The only other major crop to be so manipulated is rice. The ability produce transgenic corn gives biologists a valuable tool to probe the whys and hows of gene expression and regulation. It may also give plant breeders a way to develop new corn varieties with a speed and predictability that would be impossible with classical breeding techniques.

  17. The first satellite laser echoes recorded on the streak camera

    NASA Technical Reports Server (NTRS)

    Hamal, Karel; Prochazka, Ivan; Kirchner, Georg; Koidl, F.

    1993-01-01

    The application of the streak camera with the circular sweep for the satellite laser ranging is described. The Modular Streak Camera system employing the circular sweep option was integrated into the conventional Satellite Laser System. The experimental satellite tracking and ranging has been performed. The first satellite laser echo streak camera records are presented.

  18. Perception of Randomness: On the Time of Streaks

    ERIC Educational Resources Information Center

    Sun, Yanlong; Wang, Hongbin

    2010-01-01

    People tend to think that streaks in random sequential events are rare and remarkable. When they actually encounter streaks, they tend to consider the underlying process as non-random. The present paper examines the time of pattern occurrences in sequences of Bernoulli trials, and shows that among all patterns of the same length, a streak is the…

  19. Dark Streaks Over-riding Inactive Dunes

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Not all sand dunes on Mars are active in the modern martian environment. This example from the Lycus Sulci (Olympus Mons'aureole') region shows a case where small windblown dunes at the base of a slope have been over-ridden by more recent dark streaks (arrows). The dark streaks are most likely caused by what geologists call mass wasting or mass movement (landslides and avalanches are mass movements). Dark slope streaks such as these are common in dustier regions of Mars, and they appear to result from movement of extremely dry dust or sand in an almost fluidlike manner down a slope. This movement disrupts the bright dust coating on the surface and thus appears darker than the surrounding terrain.

    In this case, the dark slope streaks have moved up and over the dunes at the bottom of the slope, indicating that the process that moves sediment down the slope is more active (that is, it has occurred more recently and hence is more likely to occur) in the modern environment than is the movement of dunes and ripples at this location on Mars. The dunes, in fact, are probably mantled by dust. This October 1997 Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) picture is illuminated from the left and located near 31.6oN, 134.0oW.

  20. Bichromatic particle streak velocimetry bPSV

    NASA Astrophysics Data System (ADS)

    Voss, Björn; Stapf, Julian; Berthe, André; Garbe, Christoph S.

    2012-11-01

    We propose a novel technique for three-dimensional three-component (3D3C) interfacial flow measurement. It is based on the particle streak velocimetry principle. A relatively long integration time of the camera is used for capturing the movement of tracer particles as streaks on the sensor. The velocity along these streaks is extracted by periodically changing the illumination using a known pattern. A dye with different absorption characteristics in two distinct wavelengths is used to color the fluid. The depth of particles relative to the fluid interface can then be computed from their intensities when illuminated with light sources at those two different wavelengths. Hence, from our approach, a bichromatic, periodical illumination together with an image processing routine for precisely extracting particle streak features is used for measuring 3D3C fluid flow with a single camera. The technique is applied to measuring turbulent Rayleigh-Bénard convection at the free air--water interface. Using Lagrangian statistics, we are able to demonstrate a clear transition from the Batchelor regime to the Richardson regime, both of which were postulated for isotropic turbulence. The relative error of the velocity extraction of our new technique was found to be below 0.5 %.

  1. Artificial microRNA-derived resistance to Cassava brown streak disease

    PubMed Central

    Wagaba, Henry; Patil, Basavaprabhu L.; Mukasa, Settumba; Alicai, Titus; Fauquet, Claude M.; Taylor, Nigel J.

    2016-01-01

    Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3′-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20–60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava. PMID:26912232

  2. Artificial microRNA-derived resistance to Cassava brown streak disease.

    PubMed

    Wagaba, Henry; Patil, Basavaprabhu L; Mukasa, Settumba; Alicai, Titus; Fauquet, Claude M; Taylor, Nigel J

    2016-05-01

    Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3'-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20-60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava. PMID:26912232

  3. Sweet Corn Hybrid Disease Nursery - 2009

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This report summarizes the reactions of 387 sweet corn hybrids to Stewart’s wilt, common rust, maize dwarf mosaic virus, Southern leaf blight, and Northern leaf blight based on their performance in the University of Illinois sweet corn disease nursery in 2009. The reactions of these hybrids to three...

  4. Sweet Corn Hybrid Disease Nursery - 2010

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This report summarizes the reactions of 439 sweet corn hybrids to Stewart’s wilt, common rust, maize dwarf mosaic virus, Southern leaf blight, and Northern leaf blight based on their performance in the University of Illinois sweet corn disease nursery in 2010. The reactions of these hybrids to two h...

  5. Wind Streaks on Venus: Clues to Atmospheric Circulation

    NASA Technical Reports Server (NTRS)

    Greeley, Ronald; Schubert, Gerald; Limonadi, Daniel; Bender, Kelly C.; Newman, William I.; Thomas, Peggy E.; Weitz, Catherine M.; Wall, Stephen D.

    1994-01-01

    Magellan images reveal surface features on Venus attributed to wind processes. Sand dunes, wind-sculpted hills, and more than 5830 wind streaks have been identified. The streaks serve as local "wind vanes," representing wind direction at the time of streak formation and allowing the first global mapping of near-surface wind patterns on Venus. Wind streaks are oriented both toward the equator and toward the west. When streaks associated with local transient events, such as impact cratering, are deleted, the westward component is mostly lost but the equatorward component remains. This pattern is consistent with a Hadley circulation of the lower atmosphere.

  6. Photonic streaking of attosecond pulse trains

    NASA Astrophysics Data System (ADS)

    Kim, Kyung Taec; Zhang, Chunmei; Ruchon, Thierry; Hergott, Jean-François; Auguste, Thierry; Villeneuve, D. M.; Corkum, P. B.; Quéré, F.

    2013-08-01

    High harmonic radiation, produced when intense laser pulses interact with matter, is composed of a train of attosecond pulses. Individual pulses in this train carry information on ultrafast dynamics that vary from one half-optical-cycle to the next. Here, we demonstrate an all-optical photonic streaking measurement that provides direct experimental access to each attosecond pulse by mapping emission time onto propagation angle. This is achieved by inducing an ultrafast rotation of the instantaneous laser wavefront at the focus. We thus time-resolve attosecond pulse train generation, and hence the dynamics in the nonlinear medium itself. We apply photonic streaking to harmonic generation in gases and directly observe, for the first time, the influence of non-adiabatic electron dynamics and plasma formation on the generated attosecond pulse train. These experimental and numerical results also provide the first evidence of the generation of attosecond lighthouses in gases, which constitute ideal sources for attosecond pump-probe spectroscopy.

  7. Understanding baseball team standings and streaks

    NASA Astrophysics Data System (ADS)

    Sire, C.; Redner, S.

    2009-02-01

    Can one understand the statistics of wins and losses of baseball teams? Are their consecutive-game winning and losing streaks self-reinforcing or can they be described statistically? We apply the Bradley-Terry model, which incorporates the heterogeneity of team strengths in a minimalist way, to answer these questions. Excellent agreement is found between the predictions of the Bradley-Terry model and the rank dependence of the average number team wins and losses in major-league baseball over the past century when the distribution of team strengths is taken to be uniformly distributed over a finite range. Using this uniform strength distribution, we also find very good agreement between model predictions and the observed distribution of consecutive-game team winning and losing streaks over the last half-century; however, the agreement is less good for the previous half-century. The behavior of the last half-century supports the hypothesis that long streaks are primarily statistical in origin with little self-reinforcing component. The data further show that the past half-century of baseball has been more competitive than the preceding half-century.

  8. Particle Streak Velocimetry of Supersonic Nozzle Flows

    NASA Technical Reports Server (NTRS)

    Willits, J. D.; Pourpoint, T. L.

    2016-01-01

    A novel velocimetry technique to probe the exhaust flow of a laboratory scale combustor is being developed. The technique combines the advantages of standard particle velocimetry techniques and the ultra-fast imaging capabilities of a streak camera to probe high speed flows near continuously with improved spatial and velocity resolution. This "Particle Streak Velocimetry" technique tracks laser illuminated seed particles at up to 236 picosecond temporal resolution allowing time-resolved measurement of one-dimensional flows exceeding 2000 m/s as are found in rocket nozzles and many other applications. Developmental tests with cold nitrogen have been performed to validate and troubleshoot the technique with supersonic flows of much lower velocity and without background noise due to combusting flow. Flow velocities on the order of 500 m/s have been probed with titanium dioxide particles and a continuous-wave laser diode. Single frame images containing multiple streaks are analyzed to find the average slope of all incident particles corresponding to the centerline axial flow velocity. Long term objectives for these tests are correlation of specific impulse to theoretical combustion predictions and direct comparisons between candidate green fuels and the industry standard, monomethylhydrazine, each tested under identical conditions.

  9. Blisters, Calluses, and Corns

    MedlinePlus

    ... Help White House Lunch Recipes Blisters, Calluses, and Corns KidsHealth > For Kids > Blisters, Calluses, and Corns Print ... used to all of that stress. What's a Corn? Like calluses, corns are also areas of hard, ...

  10. Corns and calluses

    MedlinePlus

    Calluses and corns ... Corns and calluses are caused by pressure or friction on skin. A corn is thickened skin on the top or side ... the bunion because it rubs against the shoe. Corns and calluses are not serious problems.

  11. Signal averaging x-ray streak camera with picosecond jitter

    NASA Astrophysics Data System (ADS)

    Maksimchuk, A.; Kim, M.; Workman, J.; Korn, G.; Squier, J.; Du, D.; Umstadter, D.; Mourou, G.; Bouvier, M.

    1996-03-01

    We have developed an averaging picosecond x-ray streak camera using a dc-biased photoconductive switch as a generator of a high-voltage ramp. The streak camera is operated at a sweep speed of up to 8 ps/mm, shot-to-shot jitter is less than ±1 ps. The streak camera has been used to measure the time history of broadband x-ray emission from an ultrashort pulse laser-produced plasma. Accumulation of the streaked x-ray signals significantly improved the signal-to-noise ratio of the data obtained.

  12. Compressible laminar streaks with wall suction

    NASA Astrophysics Data System (ADS)

    Ricco, Pierre; Shah, Daniel; Hicks, Peter D.

    2013-05-01

    The response of a compressible laminar boundary layer subject to free-stream vortical disturbances and steady mean-flow wall suction is studied. The theoretical frameworks of Leib et al. [J. Fluid Mech. 380, 169-203 (1999), 10.1017/S0022112098003504] and Ricco and Wu [J. Fluid Mech. 587, 97-138 (2007), 10.1017/S0022112007007070], based on the linearized unsteady boundary-region equations, are adopted to study the influence of suction on the kinematic and thermal streaks arising through the interaction between the free-stream vortical perturbations and the boundary layer. In the asymptotic limit of small spanwise wavelength compared with the boundary layer thickness, i.e., when the disturbance flow is conveniently described by the steady compressible boundary region equations, the effect of suction is mild on the velocity fluctuations and negligible on the temperature fluctuations. When the spanwise wavelength is comparable with the boundary layer thickness, small suction values intensify the supersonic streaks, while higher transpiration levels always stabilize the disturbances at all Mach numbers. At larger spanwise wavelengths, very small amplitudes of wall transpiration have a dramatic stabilizing effect on all boundary layer fluctuations, which can take the form of transiently growing thermal streaks, large amplitude streamwise oscillations, or oblique exponentially growing Tollmien-Schlichting waves, depending on the Mach number and the wavelengths. The range of wavenumbers for which the exponential growth occurs becomes narrower and the location of instability is significantly shifted downstream by mild suction, indicating that wall transpiration can be a suitable vehicle for delaying transition when the laminar breakdown is promoted by these unstable disturbances. The typical streamwise wavelength of these disturbances is instead not influenced by suction, and asymptotic triple deck theory predicts the strong changes in growth rate and the very mild

  13. X-ray streak crystal spectography

    SciTech Connect

    Kauffman, R.L.; Brown, T.; Medecki, H.

    1983-07-01

    We have built an x-ray streaked crystal spectrograph for making time-resolved x-ray spectral measurements. This instrument can access Bragg angles from 11/sup 0/ to 38/sup 0/ and x-ray spectra from 200 eV to greater than 10 keV. We have demonstrated resolving powers, E/..delta..E > 200 at 1 keV and time resolution less than 20 psec. A description of the instrument and an example of the data is given.

  14. Mass movement slope streaks imaged by the Mars Orbiter Camera

    NASA Astrophysics Data System (ADS)

    Sullivan, Robert; Thomas, Peter; Veverka, Joseph; Malin, Michael; Edgett, Kenneth S.

    2001-10-01

    Narrow, fan-shaped dark streaks on steep Martian slopes were originally observed in Viking Orbiter images, but a definitive explanation was not possible because of resolution limitations. Pictures acquired by the Mars Orbiter Camera (MOC) aboard the Mars Global Surveyor (MGS) spacecraft show innumerable examples of dark slope streaks distributed widely, but not uniformly, across the brighter equatorial regions, as well as individual details of these features that were not visible in Viking Orbiter data. Dark slope streaks (as well as much rarer bright slope streaks) represent one of the most widespread and easily recognized styles of mass movement currently affecting the Martian surface. New dark streaks have formed since Viking and even during the MGS mission, confirming earlier suppositions that higher contrast dark streaks are younger, and fade (brighten) with time. The darkest slope streaks represent ~10% contrast with surrounding slope materials. No small outcrops supplying dark material (or bright material, for bright streaks) have been found at streak apexes. Digitate downslope ends indicate slope streak formation involves a ground-hugging flow subject to deflection by minor topographic obstacles. The model we favor explains most dark slope streaks as scars from dust avalanches following oversteepening of air fall deposits. This process is analogous to terrestrial avalanches of oversteepened dry, loose snow which produce shallow avalanche scars with similar morphologies. Low angles of internal friction typically 10-30¡ for terrestrial loess and clay materials suggest that mass movement of (low-cohesion) Martian dusty air fall is possible on a wide range of gradients. Martian gravity, presumed low density of the air fall deposits, and thin (unresolved by MOC) failed layer depths imply extremely low cohesive strength at time of failure, consistent with expectations for an air fall deposit of dust particles. As speed increases during a dust avalanche, a

  15. SDOSS: A spatially discriminating, optical streaked spectrograph

    SciTech Connect

    Cobble, J.; Evans, S.; Fernandez, J.; Oertel, J.; Watt, R.; Wilde, B.

    1995-05-01

    SDOSS is employed to study broadband laser scattering encompassing SBS, SRS, and the 3/2-{omega} signature of two plasmon decay for ns-scale laser-plasma experiments with 351 or 527-nm drive. It uses a Cassegrain telescope to image scattered light from a laser plasma onto a field stop. The telescope magnification and the stop aperture provide spatial discrimination of target plane scatter. A UV lens relays the image to a 0.25-m spectrograph which is lens coupled to a streak camera with an S-1 photocathode. The streak output is imaged onto a CCD camera. In its 512 x 480 pixel array, the CCD covers a spectral range from 200 to 800 nm with 4-nm resolution and can be adjusted to look from 350 to 1,060 nm. The sweep speed is variable with full window values of 30, 12, 6 ns, and faster. An optical fiducial provides a spectral and temporal marker. On the Livermore Nova laser, SDOSS has been used to determine spatial density in gas-filled hohlraums from SRS signals. At Trident in Los Alamos, it has been employed for similar measurements with long scale length plasmas in SBS and SRS seeding experiments. It has proven to be a versatile tool for studying the physics of laser-generated plasmas.

  16. Radar-visible wind streaks in the Altiplano of Bolivia

    NASA Technical Reports Server (NTRS)

    Greeley, R.; Christensen, P.

    1984-01-01

    Isolated knobs that are erosional remnants of central volcanoes or of folded rocks occur in several areas of the Altiplano are visible on both optical and images. The optically visible streaks occur in the immediate lee of the knobs, whereas the radar visible streaks occur in the zone downwind between the knobs. Aerial reconnaissance and field studies showed that the optically visible streaks consist of a series of small ( 100 m wide) barchan and barchanoid dunes, intradune sand sheets, and sand hummocks (large shrub coppice dunes) up to 15 m across and 5 m high. On LANDSAT images these features are poorly resolved but combine to form a bright streak. On the radar image, this area also appears brighter than the zone of the radar dark streak; evidently, the dunes and hummocks serve as radar reflectors. The radar dark streak consists of a relatively flat, smooth sand sheet which lacks organized aerolian bedforms, other than occasional ripples. Wind velocity profiles show a greater U value in the optically bright streak zone than in the radar dark streak.

  17. Corn kernel oil and corn fiber oil

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Unlike most edible plant oils that are obtained directly from oil-rich seeds by either pressing or solvent extraction, corn seeds (kernels) have low levels of oil (4%) and commercial corn oil is obtained from the corn germ (embryo) which is an oil-rich portion of the kernel. Commercial corn oil cou...

  18. Efficient transmission of Cassava brown streak disease viral pathogens by chip bud grafting

    PubMed Central

    2013-01-01

    Background Techniques to study plant viral diseases under controlled growth conditions are required to fully understand their biology and investigate host resistance. Cassava brown streak disease (CBSD) presents a major threat to cassava production in East Africa. No infectious clones of the causal viruses, Cassava brown streak virus (CBSV) or Ugandan cassava brown streak virus (UCBSV) are available, and mechanical transmission to cassava is not effective. An improved method for transmission of the viruses, both singly and as co-infections has been developed using bud grafts. Findings Axillary buds from CBSD symptomatic plants infected with virulent isolates of CBSV and UCBSV were excised and grafted onto 6–8 week old greenhouse-grown, disease-free cassava plants of cultivars Ebwanateraka, TME204 and 60444. Plants were assessed visually for development of CBSD symptoms and by RT-PCR for presence of the viruses in leaf and storage root tissues. Across replicated experiments, 70-100% of plants inoculated with CBSV developed CBSD leaf and stem symptoms 2–6 weeks after bud grafting. Infected plants showed typical, severe necrotic lesions in storage roots at harvest 12–14 weeks after graft inoculation. Sequential grafting of buds from plants infected with UCBSV followed 10–14 days later by buds carrying CBSV, onto the same test plant, resulted in 100% of the rootstocks becoming co-infected with both pathogens. This dual transmission rate was greater than that achieved by simultaneous grafting with UCBSV and CBSV (67%), or when grafting first with CBSV followed by UCBSV (17%). Conclusions The bud grafting method described presents an improved tool for screening cassava germplasm for resistance to CBSD causal viruses, and for studying pathogenicity of this important disease. Bud grafting provides new opportunities compared to previously reported top and side grafting systems. Test plants can be inoculated as young, uniform plants of a size easily handled in a

  19. Streak interactions and breakdown in boundary layer flows

    NASA Astrophysics Data System (ADS)

    Brandt, Luca; de Lange, H. C.

    2008-02-01

    The objective of this paper is to show that the interaction of streamwise velocity streaks of finite length can lead to turbulent breakdown in the flat-plate boundary layer flow. The work is motivated by previous numerical and experimental studies of transitional flows where the high-frequency oscillations leading to turbulence are seen to form in the region of strongest shear induced by streaks in relative motion. Therefore, a model for the interaction of steady and unsteady (i.e., slowly moving in the spanwise direction) spanwise periodic streaks is proposed. The interaction of two subsequent streaks is investigated for varying collision parameters. In particular, the relative spanwise position and angle are considered. The results show that the interaction is able to produce both a symmetric and asymmetric breakdown without the need for additional random noise from the main stream. Velocity structures characteristic of both scenarios are analyzed. Hairpin and Λ vortices are found in the case of symmetric collision between a low-speed region and an incoming high-speed streak, when a region of strong wall-normal shear is induced. Alternatively, when the incoming high-momentum fluid is misaligned with the low-speed streak in front, single quasi-streamwise vortices are identified. Despite the different symmetry at the breakdown, the detrimental interaction involves for both cases the tail of a low-speed region and the head of a high-speed streak. Further, the breakdown appears in both scenarios as an instability of three-dimensional shear layers formed between the two streaks. The streak interaction scenario is suggested to be of relevance for turbulence production in wall-bounded flows.

  20. High Performance Imaging Streak Camera for the National Ignition Facility

    SciTech Connect

    Opachich, Y. P.; Kalantar, D.; MacPhee, A.; Holder, J.; Kimbrough, J.; Bell, P. M.; Bradley, D.; Hatch, B.; Brown, C.; Landen, O.; Perfect, B. H.; Guidry, B.; Mead, A.; Charest, M.; Palmer, N.; Homoelle, D.; Browning, D.; Silbernagel, C.; Brienza-Larsen, G.; Griffin, M.; Lee, J. J.; Haugh, M. J.

    2012-01-01

    An x-ray streak camera platform has been characterized and implemented for use at the National Ignition Facility. The camera has been modified to meet the experiment requirements of the National Ignition Campaign and to perform reliably in conditions that produce high EMI. A train of temporal UV timing markers has been added to the diagnostic in order to calibrate the temporal axis of the instrument and the detector efficiency of the streak camera was improved by using a CsI photocathode. The performance of the streak camera has been characterized and is summarized in this paper. The detector efficiency and cathode measurements are also presented.

  1. Temporal Contrast Changes in Dark Slope Streaks on Mars

    NASA Astrophysics Data System (ADS)

    Chilton, H.; Phillips, C. B.; Fenton, L. K.; Brown, A. J.

    2012-12-01

    Dark slope streaks on Mars, first observed in Viking images, provide insight into one of the most active and dynamic processes observed on the planet's surface. While various formation models have been suggested [1][2][3], dust avalanches seem to best explain streak origin and characteristics[4][5]. New dark streaks are observed to have the greatest contrast to their surroundings while older streaks have lower contrast, suggesting that streaks fade over time. One theory for this is atmospheric dust fallout slowly raising the albedo of the surface exposed by the dust avalanche, resulting in increased streak albedo over time until the streak becomes indistinguishable from the surrounding surface. In this study, we attempt an initial evaluation of changes in streak brightness relative to surroundings, with a first order correction for incidence angle[6] based on MOLA data. CRISM images were first identified for spatial and temporal overlap, then further selected for those image sets with well-matched viewing geometries. Locations included Nicholson Crater (CRISM images: frt0000c287_07_de165l, hrl0000d0f1_7_de165l, frt00018c69_07_de165l) and South of Nestus Valles (CRISM images: hrl00004a5e_07_de181l, hrl0000812a_07_de182l) as well as Naktong Vallis (CRISM images: hrl0000898d_07_de182l, hrl00005337_07_de182l) and an area in Lycus Sulci (CRISM images: hrl0000a52a_07_de166l, hrl0000ce5f_07_if175l). We focused on 1 micron wavelength CRISM images in order to reduce atmosphric interference. From here, brightness (observed radiance divided by solar irradiance at Mars divided by pi) values were collected along individual streaks, with measurements at multiple locations along the streak length and alongside at points of similar elevation to streak measurements to establish an average contrast ratio. Both on-streak and off-streak values were divided by the cosine of their respective local MOLA incidence angles to correct for brightness variation due to solar flux and

  2. Transgenic RNA interference (RNAi)-derived field resistance to cassava brown streak disease.

    PubMed

    Ogwok, Emmanuel; Odipio, John; Halsey, Mark; Gaitán-Solís, Eliana; Bua, Anton; Taylor, Nigel J; Fauquet, Claude M; Alicai, Titus

    2012-12-01

    Cassava brown streak disease (CBSD), caused by the Ipomoviruses Cassava brown streak virus (CBSV) and Ugandan Cassava brown streak virus (UCBSV), is considered to be an imminent threat to food security in tropical Africa. Cassava plants were transgenically modified to generate small interfering RNAs (siRNAs) from truncated full-length (894-bp) and N-terminal (402-bp) portions of the UCBSV coat protein (ΔCP) sequence. Seven siRNA-producing lines from each gene construct were tested under confined field trials at Namulonge, Uganda. All nontransgenic control plants (n = 60) developed CBSD symptoms on aerial tissues by 6 months after planting, whereas plants transgenic for the full-length ΔCP sequence showed a 3-month delay in disease development, with 98% of clonal replicates within line 718-001 remaining symptom free over the 11-month trial. Reverse transcriptase-polymerase chain reaction (RT-PCR) diagnostics indicated the presence of UCBSV within the leaves of 57% of the nontransgenic controls, but in only two of 413 plants tested (0.5%) across the 14 transgenic lines. All transgenic plants showing CBSD were PCR positive for the presence of CBSV, except for line 781-001, in which 93% of plants were confirmed to be free of both pathogens. At harvest, 90% of storage roots from nontransgenic plants were severely affected by CBSD-induced necrosis. However, transgenic lines 718-005 and 718-001 showed significant suppression of disease, with 95% of roots from the latter line remaining free from necrosis and RT-PCR negative for the presence of both viral pathogens. Cross-protection against CBSV by siRNAs generated from the full-length UCBSV ΔCP confirms a previous report in tobacco. The information presented provides proof of principle for the control of CBSD by RNA interference-mediated technology, and progress towards the potential control of this damaging disease. PMID:22845735

  3. Active Processes: Bright Streaks and Dark Fans

    NASA Technical Reports Server (NTRS)

    2007-01-01

    [figure removed for brevity, see original site] [figure removed for brevity, see original site] Figure 1Figure 2

    In a region of the south pole known informally as 'Ithaca' numerous fans of dark frost form every spring. HiRISE collected a time lapse series of these images, starting at Ls = 185 and culminating at Ls = 294. 'Ls' is the way we measure time on Mars: at Ls = 180 the sun passes the equator on its way south; at Ls = 270 it reaches its maximum subsolar latitude and summer begins.

    In the earliest image (figure 1) fans are dark, but small narrow bright streaks can be detected. In the next image (figure 2), acquired at Ls = 187, just 106 hours later, dramatic differences are apparent. The dark fans are larger and the bright fans are more pronounced and easily detectable. The third image in the sequence shows no bright fans at all.

    We believe that the bright streaks are fine frost condensed from the gas exiting the vent. The conditions must be just right for the bright frost to condense.

    Observation Geometry Image PSP_002622_0945 was taken by the High Resolution Imaging Science Experiment (HiRISE) camera onboard the Mars Reconnaissance Orbiter spacecraft on 16-Feb-2007. The complete image is centered at -85.2 degrees latitude, 181.5 degrees East longitude. The range to the target site was 246.9 km (154.3 miles). At this distance the image scale is 49.4 cm/pixel (with 2 x 2 binning) so objects 148 cm across are resolved. The image shown here has been map-projected to 50 cm/pixel . The image was taken at a local Mars time of 05:46 PM and the scene is illuminated from the west with a solar incidence angle of 88 degrees, thus the sun was about 2 degrees above the horizon. At a solar longitude of 185.1 degrees, the season on Mars is Northern Autumn.

  4. Elimination of five sugarcane viruses from sugarcane using in vitro culture of axillary bud and apical meristem

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Procedures were developed for the in vitro elimination of Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Sugarcane streak mosaic virus (SCSMV), Sugarcane yellow leaf virus (SCYLV) and Fiji disease virus (FDV) from infected sugarcane. In vitro shoot regeneration, elongation and virus el...

  5. Temporal Contrast Changes in Dark Slope Streaks on Mars

    NASA Astrophysics Data System (ADS)

    Chilton, Heather; Phillips, C. B.; Brown, A.; Fenton, L.

    2013-01-01

    Dark slope streaks on Mars are among the most active and dynamic processes observed on the planet's surface. While various formation models have been suggested [1][2][3], dust avalanches seem to best explain streak origin and characteristics[4][5]. New streaks have the highest contrast suggesting they fade over time. One theory for this is atmospheric dust fallout slowly raising the albedo of the surface exposed by the dust avalanche, progressively increasing streak albedo until the streak becomes indistinguishable from the surrounding surface. In this study, we attempt an initial evaluation of changes in streak brightness relative to surroundings with a first order correction for incidence angle[6]. CRISM images were identified for spatial overlap and temporal range, then further selected for image sets with well-matched viewing geometries. The 1 micron wavelength band was used to reduce atmospheric interference and brightness values then collected at multiple locations within and alongside individual streaks, with on-streak/off-streak pairs at points of similar elevation. Values were then divided by the cosine of the corresponding MOLA incidence angle for brightness variation correction due to topography. Measurements for overlapping images established local and overall averages for the rate of change in contrast ratio. Results showed a range of trends including streaks that darkened, brightened, brightened then darkened or vice versa. We continue to explore the possibility of non-linear brightening as well as streak reactivation and localized events, surface characteristics, and topography. Further study will focus on these and other morphological changes observed from vast data sets of other instruments including the Mars Reconnaissance Orbter HiRISE and CTX, Mars Express HRSC, Mars Odyssey THEMIS (visible), and the Mars Global Surveyor MOC. [1] Morris (1982) JGR, 87, 1164-1178. [2] Ferguson and Lucchita (1984) NASA Tech. Memo., TM-86246, 188-190. [3] Miyamoto

  6. The genome organization of lucerne transient streak and turnip rosette sobemoviruses revisited.

    PubMed

    Sõmera, Merike; Truve, Erkki

    2013-03-01

    Unlike other sobemoviruses, lucerne transient streak virus (LTSV) and turnip rosette virus (TRoV) have been reported to contain two successive ORF1s (denoted as ORF1a and ORF1b) instead of a single ORF1. Also, their next ORF (ORF2a/2a2b) has been mapped to a region ca. 200 nucleotides downstream from that of other sobemoviruses, leading to the lack of transmembrane segments at the N-termini of P2a/2a2b. In the current study, we resequenced this region for TRoV and LTSV. The hypothetical beginning of ORF1b was mapped as the beginning of ORF2a/2a2b for both TRoV and LTSV. Computional analysis revealed transmembrane segments at the N-termini of the TRoV and LTSV polyproteins. PMID:23111554

  7. Semiclassical model for attosecond angular streaking.

    PubMed

    Smolarski, M; Eckle, P; Keller, U; Dörner, R

    2010-08-16

    Attosecond angular streaking is a new technique to achieve unsurpassed time accuracy of only a few attoseconds. Recently this has been successfully used to set an upper limit on the electron tunneling delay time in strong laser field ionization. The measurement technique can be modeled with either the time-dependent Schrödinger equation (TDSE) or a more simple semiclassical approach that describes the process in two steps in analogy to the three-step model in high harmonic generation (HHG): step one is the tunnel ionization and step two is the classical motion in the strong laser field. Here we describe in detail a semiclassical model which is based on the ADK theory for the tunneling step, with subsequent classical propagation of the electron in the laser field. We take into account different ellipticities of the laser field and a possible wavelength-dependent ellipticity that is typically observed for pulses in the two-optical-cycle regime. This semiclassical model shows excellent agreement with the experimental result. PMID:20721150

  8. Simulation of FEL pulse length calculation with THz streaking method.

    PubMed

    Gorgisyan, I; Ischebeck, R; Prat, E; Reiche, S; Rivkin, L; Juranić, P

    2016-05-01

    Having accurate and comprehensive photon diagnostics for the X-ray pulses delivered by free-electron laser (FEL) facilities is of utmost importance. Along with various parameters of the photon beam (such as photon energy, beam intensity, etc.), the pulse length measurements are particularly useful both for the machine operators to measure the beam parameters and monitor the stability of the machine performance, and for the users carrying out pump-probe experiments at such facilities to better understand their measurement results. One of the most promising pulse length measurement techniques used for photon diagnostics is the THz streak camera which is capable of simultaneously measuring the lengths of the photon pulses and their arrival times with respect to the pump laser. This work presents simulations of a THz streak camera performance. The simulation procedure utilizes FEL pulses with two different photon energies in hard and soft X-ray regions, respectively. It recreates the energy spectra of the photoelectrons produced by the photon pulses and streaks them by a single-cycle THz pulse. Following the pulse-retrieval procedure of the THz streak camera, the lengths were calculated from the streaked spectra. To validate the pulse length calculation procedure, the precision and the accuracy of the method were estimated for streaking configuration corresponding to previously performed experiments. The obtained results show that for the discussed setup the method is capable of measuring FEL pulses with about a femtosecond accuracy and precision. PMID:27140142

  9. Simulation of FEL pulse length calculation with THz streaking method

    PubMed Central

    Gorgisyan, I.; Ischebeck, R.; Prat, E.; Reiche, S.; Rivkin, L.; Juranić, P.

    2016-01-01

    Having accurate and comprehensive photon diagnostics for the X-ray pulses delivered by free-electron laser (FEL) facilities is of utmost importance. Along with various parameters of the photon beam (such as photon energy, beam intensity, etc.), the pulse length measurements are particularly useful both for the machine operators to measure the beam parameters and monitor the stability of the machine performance, and for the users carrying out pump–probe experiments at such facilities to better understand their measurement results. One of the most promising pulse length measurement techniques used for photon diagnostics is the THz streak camera which is capable of simultaneously measuring the lengths of the photon pulses and their arrival times with respect to the pump laser. This work presents simulations of a THz streak camera performance. The simulation procedure utilizes FEL pulses with two different photon energies in hard and soft X-ray regions, respectively. It recreates the energy spectra of the photoelectrons produced by the photon pulses and streaks them by a single-cycle THz pulse. Following the pulse-retrieval procedure of the THz streak camera, the lengths were calculated from the streaked spectra. To validate the pulse length calculation procedure, the precision and the accuracy of the method were estimated for streaking configuration corresponding to previously performed experiments. The obtained results show that for the discussed setup the method is capable of measuring FEL pulses with about a femtosecond accuracy and precision. PMID:27140142

  10. PHYLOGENETIC STUDIES OF CORN AND RICE STRAINS OF ACIDOVORAX AVENAE SUBSP. AVENAE BY DNA/DNA HYBRIDIZATION

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Acidovorax avenae subsp. avenae (Aaa) is the causal agent of diseases of several important economic crops, including bacterial streak of corn (Zea mays) and bacterial stripe of rice (Oryza sativa). To determine the phylogenetic relationship of these two pathogens, a highly reproducible S1 exonuclea...

  11. Study of Geometric Parameters of Slope Streaks on Mars.

    NASA Astrophysics Data System (ADS)

    Brusnikin, Eugene; Kreslavsky, Mikhail; Karachevtseva, Irina; Zubarev, Anatoliy; Patratiy, Vyacheslav

    2015-04-01

    Slope streaks are a unique active phenomenon observed in low-latitude dusty regions on Mars. They are dark markings formed by an unknown type of run-away downslope propagation of surface disturbance. There are two kinds of hypotheses of their formation mechanism: "dry", involving granular follow, in particular, dust avalanche, and "wet", involving liquid flow, in particular, percolation of concentrated brines in shallow subsurface (1). Study of geometric characteristics of the slope streaks, especially their slopes, is a way to decipher their origin. We are carrying out an extensive set of measurements of geometric parameters of the slope streaks. We use stereo pairs of images obtained by High Resolution Imaging Science Experiment (HiRISE) onboard MRO orbital mission to Mars. These stereo pairs potentially allow geometric measurements (both horizontal and vertical) with accuracy on an order of a meter. Unfortunately, the digital terrain model is currently released for only one stereo pair in the regions of slope streak occurrence, and we have to work with raw, unprocessed stereo pairs. We perform direct photogrammetric measurements using PHOTOMOD software complex (http://www.racurs.ru/). We use our custom software to import "raw" HiRISE imgas (EDRs) and supplementary geometric information from SPICE into PHOTOMOD (2). We select tens to a hundred meters long segments in the beginning and the end of selected streaks and register length, azimuth, and slope of each segment. We also search for anomalously gentle parts of streaks. We analyze the obtained results by means of ESRI ArcGIS software. Our survey is in progress. So far we registered over a hundred of streaks. We found that the extent of the streaks varies from several meters to hundreds of meters. The streaks are formed in locales with a slope from 17 to 37 degrees. The lower boundary indicates that the streaks can propagate on slopes that are significantly gentler than the static angle of repose. Distal

  12. Streak detection and analysis pipeline for optical images

    NASA Astrophysics Data System (ADS)

    Virtanen, J.; Granvik, M.; Torppa, J.; Muinonen, K.; Poikonen, J.; Lehti, J.; Säntti, T.; Komulainen, T.; Flohrer, T.

    2014-07-01

    We describe a novel data processing and analysis pipeline for optical observations of moving objects, either of natural (asteroids, meteors) or artificial origin (satellites, space debris). The monitoring of the space object populations requires reliable acquisition of observational data to support the development and validation of population models, and to build and maintain catalogues of orbital elements. The orbital catalogues are, in turn, needed for the assessment of close approaches (for asteroids, with the Earth; for satellites, with each other) and for the support of contingency situations or launches. For both types of populations, there is also increasing interest to detect fainter objects corresponding to the small end of the size distribution. We focus on the low signal-to-noise (SNR) detection of objects with high angular velocities, resulting in long and faint object trails, or streaks, in the optical images. The currently available, mature image processing algorithms for detection and astrometric reduction of optical data cover objects that cross the sensor field-of-view comparably slowly, and, particularly for satellites, within a rather narrow, predefined range of angular velocities. By applying specific tracking techniques, the objects appear point-like or as short trails in the exposures. However, the general survey scenario is always a 'track-before-detect' problem, resulting in streaks of arbitrary lengths. Although some considerations for low-SNR processing of streak-like features are available in the current image processing and computer vision literature, algorithms are not readily available yet. In the ESA-funded StreakDet (Streak detection and astrometric reduction) project, we develop and evaluate an automated processing pipeline applicable to single images (as compared to consecutive frames of the same field) obtained with any observing scenario, including space-based surveys and both low- and high-altitude populations. The algorithmic

  13. Hitting Is Contagious in Baseball: Evidence from Long Hitting Streaks

    PubMed Central

    Bock, Joel R.; Maewal, Akhilesh; Gough, David A.

    2012-01-01

    Data analysis is used to test the hypothesis that “hitting is contagious”. A statistical model is described to study the effect of a hot hitter upon his teammates’ batting during a consecutive game hitting streak. Box score data for entire seasons comprising streaks of length games, including a total observations were compiled. Treatment and control sample groups () were constructed from core lineups of players on the streaking batter’s team. The percentile method bootstrap was used to calculate confidence intervals for statistics representing differences in the mean distributions of two batting statistics between groups. Batters in the treatment group (hot streak active) showed statistically significant improvements in hitting performance, as compared against the control. Mean for the treatment group was found to be to percentage points higher during hot streaks (mean difference increased points), while the batting heat index introduced here was observed to increase by points. For each performance statistic, the null hypothesis was rejected at the significance level. We conclude that the evidence suggests the potential existence of a “statistical contagion effect”. Psychological mechanisms essential to the empirical results are suggested, as several studies from the scientific literature lend credence to contagious phenomena in sports. Causal inference from these results is difficult, but we suggest and discuss several latent variables that may contribute to the observed results, and offer possible directions for future research. PMID:23251507

  14. Design and Field Test of a Galvanometer Deflected Streak Camera

    SciTech Connect

    Lai, C C; Goosman, D R; Wade, J T; Avara, R

    2002-11-08

    We have developed a compact fieldable optically-deflected streak camera first reported in the 20th HSPP Congress. Using a triggerable galvanometer that scans the optical signal, the imaging and streaking function is an all-optical process without incurring any photon-electron-photon conversion or photoelectronic deflection. As such, the achievable imaging quality is limited mainly only by optical design, rather than by multiple conversions of signal carrier and high voltage electron-optics effect. All core elements of the camera are packaged into a 12 inch x 24 inch footprint box, a size similar to that of a conventional electronic streak camera. At LLNL's Site-300 Test Site, we have conducted a Fabry-Perot interferometer measurement of fast object velocity using this all-optical camera side-by-side with an intensified electronic streak camera. These two cameras are configured as two independent instruments for recording synchronously each branch of the 50/50 splits from one incoming signal. Given the same signal characteristics, the test result has undisputedly demonstrated superior imaging performance for the all-optical streak camera. It produces higher signal sensitivity, wider linear dynamic range, better spatial contrast, finer temporal resolution, and larger data capacity as compared with that of the electronic counterpart. The camera had also demonstrated its structural robustness and functional consistence to be well compatible with field environment. This paper presents the camera design and the test results in both pictorial records and post-process graphic summaries.

  15. The Dellistrique 2S - A 200 Channel Streak Camera

    NASA Astrophysics Data System (ADS)

    Majumdar, S.

    1985-02-01

    Despite a great deal of effort to improve time resolution of streak cameras, the best reported time resolution remained just under one picosecond for more than a decade. However, the sensitivity and resolution of these devices have improved substantially over this time. In this paper the design of a very high performance streak camera, capable of resolving one picosecond on 200 parallel channels with single photo-electron detection capability: the Dellistrique 2S. The system comprises of a streak tube type Picotron 200 with a photocathode resolving 200 spatial channels along a slit and with an extraction field near the photocathode of greater than 16 KV per cm and with a non saturating phosphor screen as the main component. There is post streak tube intensification of 30,000 at the streak tube output wavelength which increases both the sensitivity of the system. The intensifier is coupled to a CCD readout device fibre-optically for producing a two dimensional image with a dynamic range of greater than 500. The camera can be operated at a repetition rate of between 80 MHz and 240 MHz using R.F. scanning methods. Upto 20 parallel channels have been tried with parallel information recording, which is well below the tube's capability of recording 200 independent channels. The image is virtually distortion free across the whole usable screen length of 45 mm for a large screen tube.

  16. A time-resolved image sensor for tubeless streak cameras

    NASA Astrophysics Data System (ADS)

    Yasutomi, Keita; Han, SangMan; Seo, Min-Woong; Takasawa, Taishi; Kagawa, Keiichiro; Kawahito, Shoji

    2014-03-01

    This paper presents a time-resolved CMOS image sensor with draining-only modulation (DOM) pixels for tube-less streak cameras. Although the conventional streak camera has high time resolution, the device requires high voltage and bulky system due to the structure with a vacuum tube. The proposed time-resolved imager with a simple optics realize a streak camera without any vacuum tubes. The proposed image sensor has DOM pixels, a delay-based pulse generator, and a readout circuitry. The delay-based pulse generator in combination with an in-pixel logic allows us to create and to provide a short gating clock to the pixel array. A prototype time-resolved CMOS image sensor with the proposed pixel is designed and implemented using 0.11um CMOS image sensor technology. The image array has 30(Vertical) x 128(Memory length) pixels with the pixel pitch of 22.4um. .

  17. Attosecond streaking in a nano-plasmonic field

    NASA Astrophysics Data System (ADS)

    Kelkensberg, F.; Koenderink, A. F.; Vrakking, M. J. J.

    2012-09-01

    A theoretical study of the application of attosecond streaking spectroscopy to time-resolved studies of the plasmonic fields surrounding isolated, resonantly excited spherical nanoparticles is presented. A classification of the different regimes in attosecond streaking is proposed and identified in our results that are derived from Mie calculations of plasmon fields, coupled to classical electron trajectory simulations. It is shown that in an attosecond streaking experiment, the electrons are almost exclusively sensitive to the component of the field parallel to the direction in which they are detected. This allows one to probe the different components of the field individually by resolving the angle of emission of the electrons. Finally, simulations based on fields calculated by finite-difference time-domain (FDTD) are compared with the results obtained using Mie fields. The two are found to be in good agreement with each other, supporting the notion that FDTD methods can be used to reliably investigate non-spherical structures.

  18. Mead Crater, Venus - Aerodynamic roughness of wind streaks

    NASA Astrophysics Data System (ADS)

    Williams, K. K.; Greeley, R.

    1997-03-01

    Radar backscatter images of Venus returned by the Magellan spacecraft revealed many aeolian features on the planet's surface. While much work has focused on terrestrial wind streaks, the harsh environment of Venus limits direct measurement of surface properties, such as aerodynamic roughness, that affect aeolian features on that planet. However, a correlation between radar backscatter and aerodynamic roughness (Z0) for the S-band radar system on Magellan can be used to study the aerodynamic roughnesses of areas in which Venusian wind streaks occur. The aerodynamic roughness of areas with both radar-bright and radar-dark wind streaks near Mead crater are calculated and compared to z0 values measured on Earth in order to compare the surface of Venus with known terrestrial surface textures.

  19. Triticum mosaic virus isolates in the southern Great Plains

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In 2006, a Wheat streak mosaic virus (WSMV)-resistant wheat variety RonL was found to have mosaic symptoms similar to WSMV. The virus inducing the symptoms was determined to be previously unknown and given the name Triticum mosaic virus (TriMV). Since, TriMV has been found in plant samples isolate...

  20. Complete genome sequence of the alfalfa latent virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Alfalfa latent virus (ALV) is a member of the carlavirus group and occurs symptomlessly in alfalfa (Medicago sativa). In the US it is prevalent in Nebraska and Wisconsin. The virus is recognized as a strain of Pea streak virus (PeSV) So far, no complete genomic sequence of PSV or ALV is availab...

  1. An Experimental Host Range of Triticum Mosaic Virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Triticum mosaic virus (TriMV) is a newly discovered virus isolated from wheat. This study was conducted to determine an experimental host range for TriMV and identify species that could serve as differential hosts for isolating TriMV from Wheat streak mosaic virus (WSMV). Plants tested were mechan...

  2. Streak Camera Performance with Large-Format CCD Readout

    SciTech Connect

    Lerche, R A; Andrews, D S; Bell, P M; Griffith, R L; McDonald, J W; Torres, P III; Vergel de Dios, G

    2003-07-08

    The ICF program at Livermore has a large inventory of optical streak cameras that were built in the 1970s and 1980s. The cameras include micro-channel plate image-intensifier tubes (IIT) that provide signal amplification and early lens-coupled CCD readouts. Today, these cameras are still very functional, but some replacement parts such as the original streak tube, CCD, and IIT are scarce and obsolete. This article describes recent efforts to improve the performance of these cameras using today's advanced CCD readout technologies. Very sensitive, large-format CCD arrays with efficient fiber-optic input faceplates are now available for direct coupling with the streak tube. Measurements of camera performance characteristics including linearity, spatial and temporal resolution, line-spread function, contrast transfer ratio (CTR), and dynamic range have been made for several different camera configurations: CCD coupled directly to the streak tube, CCD directly coupled to the IIT, and the original configuration with a smaller CCD lens coupled to the IIT output. Spatial resolution (limiting visual) with and without the IIT is 8 and 20 lp/mm, respectively, for photocathode current density up to 25% of the Child-Langmuir (C-L) space-charge limit. Temporal resolution (fwhm) deteriorates by about 20% when the cathode current density reaches 10% of the C-L space charge limit. Streak tube operation with large average tube current was observed by illuminating the entire slit region through a Ronchi ruling and measuring the CTR. Sensitivity (CCD electrons per streak tube photoelectron) for the various configurations ranged from 7.5 to 2,700 with read noise of 7.5 to 10.5 electrons. Optimum spatial resolution is achieved when the IIT is removed. Maximum dynamic range requires a configuration where a single photoelectron from the photocathode produces a signal that is 3 to 5 times the read noise.

  3. Delaying corn rootworm resistance to Bt corn.

    PubMed

    Tabashnik, Bruce E; Gould, Fred

    2012-06-01

    Transgenic crops producing Bacillus thuringiensis (Bt) toxins for insect control have been successful, but their efficacy is reduced when pests evolve resistance. To delay pest resistance to Bt crops, the U.S. Environmental Protection Agency (EPA) has required refuges of host plants that do not produce Bt toxins to promote survival of susceptible pests. Such refuges are expected to be most effective if the Bt plants deliver a dose of toxin high enough to kill nearly all hybrid progeny produced by matings between resistant and susceptible pests. In 2003, the EPA first registered corn, Zea mays L., producing a Bt toxin (Cry3Bb1) that kills western corn rootworm, Diabrotica virgifera virgifera LeConte, one of the most economically important crop pests in the United States. The EPA requires minimum refuges of 20% for Cry3Bb1 corn and 5% for corn producing two Bt toxins active against corn rootworms. We conclude that the current refuge requirements are not adequate, because Bt corn hybrids active against corn rootworms do not meet the high-dose standard, and western corn rootworm has rapidly evolved resistance to Cry3Bb1 corn in the laboratory, greenhouse, and field. Accordingly, we recommend increasing the minimum refuge for Bt corn targeting corn rootworms to 50% for plants producing one toxin active against these pests and to 20% for plants producing two toxins active against these pests. Increasing the minimum refuge percentage can help to delay pest resistance, encourage integrated pest management, and promote more sustainable crop protection. PMID:22812111

  4. Our Mother Corn.

    ERIC Educational Resources Information Center

    Mathers, Sherry; And Others

    Developed to provide an understanding of the magnitude of the role of corn, referred to as Mother Corn in the cultures of the Seneca, Pawnee, and Hopi tribes, the student text provides information on the tribes' basic lifestyles and the way they grew and used corn in three different parts of the United States. The section on the origin of corn…

  5. Hot streaks and phantom cooling in a turbine rotor passage. I - Separate effects

    NASA Technical Reports Server (NTRS)

    Roback, Richard J.; Dring, Robert P.

    1992-01-01

    Experimental documentation and analytical correlations demonstrating the effects of hot streak accumulation and phantom cooling on turbine rotor airfoil surface temperature. Test results are shown for a range of controlling variables to identify where streak accumulation and phantom cooling are most likely to be strongest. These variables include streak injection location, streak-to-free stream density ratio and coolant-to-free stream density and velocity ratios.

  6. Processing Particle-Streak Imagery On A Personal Computer

    NASA Technical Reports Server (NTRS)

    Cho, Y. C.; Mclachlan, B. G.

    1991-01-01

    Report describes simple flow experiment in which streak images of particles suspended in fluid and illuminated for short times processed into maps of velocity vectors. In experiment, semiautomatic data-reduction scheme used which recovers significant image data more efficiently than currently available automatic scheme.

  7. Soft x-ray streak camera for laser fusion applications

    SciTech Connect

    Stradling, G.L.

    1981-04-01

    This thesis reviews the development and significance of the soft x-ray streak camera (SXRSC) in the context of inertial confinement fusion energy development. A brief introduction of laser fusion and laser fusion diagnostics is presented. The need for a soft x-ray streak camera as a laser fusion diagnostic is shown. Basic x-ray streak camera characteristics, design, and operation are reviewed. The SXRSC design criteria, the requirement for a subkilovolt x-ray transmitting window, and the resulting camera design are explained. Theory and design of reflector-filter pair combinations for three subkilovolt channels centered at 220 eV, 460 eV, and 620 eV are also presented. Calibration experiments are explained and data showing a dynamic range of 1000 and a sweep speed of 134 psec/mm are presented. Sensitivity modifications to the soft x-ray streak camera for a high-power target shot are described. A preliminary investigation, using a stepped cathode, of the thickness dependence of the gold photocathode response is discussed. Data from a typical Argus laser gold-disk target experiment are shown.

  8. Silent polypoidal choroidal vasculopathy in a patient with angioid streaks.

    PubMed

    Cebeci, Zafer; Bayraktar, Serife; Oray, Merih; Kir, Nur

    2016-01-01

    We present a case of silent polypoidal choroidal vasculopathy (PCV) in a patient with angioid streaks. PCV was detected during a routine ophthalmic examination and confirmed by fluorescein angiography, indocyanine green angiography, and optical coherence tomography. After 2 years of follow-up, the PCV remained silent without any complications. We report this rare coexistence and review literature on this topic. PMID:27463636

  9. Improved approach to characterizing and presenting streak camera performance

    SciTech Connect

    Wiedwald, J.D.; Jones, B.A.

    1985-07-15

    The performance of a streak camera recording system is strongly linked to the technique used to amplify, detect and quantify the streaked image. At the Lawrence Livermore National Laboratory (LLNL) streak camera images have been recorded both on film and by fiber-optically coupling to charge-coupled devices (CCD's). During the development of a new process for recording these images (lens coupling the image onto a cooled CCD) the definitions of important performance characteristics such as resolution and dynamic range were re-examined. As a result of this development, these performance characteristics are now presented to the streak camera user in a more useful format than in the past. This paper describes how these techniques are used within the Laser Fusion Program at LLNL. The system resolution is presented as a modulation transfer function, including the seldom reported effects that flare and light scattering have at low spatial frequencies. Data are presented such that a user can adjust image intensifier gain and pixel averaging to optimize the useful dynamic range in any particular application.

  10. Analysis of hot streak effects on turbine rotor heat load

    SciTech Connect

    Shang, T.; Epstein, A.H.

    1997-07-01

    The influence of inlet hot streak temperature distortion on turbine blade heat load was explored on a transonic axial flow turbine stage test article using a three-dimensional, multiblade row unsteady Euler code. The turbine geometry was the same as that used for a recently reported testing of hot streak influence. Emphasis was placed on elucidating the physical and mechanisms by which hot streaks affect turbine durability. It was found that temperature distortion significantly increases both blade surface heat load nonuniformity and total blade heat load by as much as 10--30% (mainly in the pressure surface), and that the severity of this influence is a strong function of turbine geometry and flow conditions. Three physical mechanisms were identified that drive the heat load nonuniformity: buoyancy, wake convection (the Kerrebrock-Mikolajczak effect), and rotor-stator interactions. The latter can generate significant nonuniformity of the time-averaged relative frame rotor inlet temperature distribution. Dependence of these effects on turbine design variables was investigated to shed light on the design space, which minimizes the adverse effects of hot streaks.

  11. Attosecond time-resolved streaked photoemission from solids

    NASA Astrophysics Data System (ADS)

    Liao, Qing; Thumm, Uwe

    2015-05-01

    We established a quantum-mechanical model for infrared (IR) laser streaked photoelectron (PE) emission from metal solids by an ultrashort extreme ultraviolet (XUV) pulse. Special emphasis was laid on the influence of the energy dispersion of PEs inside the solids on the photoemission time delay. We first applied this model to Mg(0001) surfaces, assuming free-electron dispersion and found good agreement with measured streaked PE spectra and streaking time delays. Next, we investigate W(110) surfaces for which non-free PE dispersion must be included in order to reproduce the measured photoemission delays at different XUV central photon energies. Our model reproduces a series of measured streaked spectrograms and photoemission delays for different metal solids, including clean Mg(0001) and W(110) surfaces and Mg-covered W(110) surfaces. It incorporates modeling of the target band structure, electron mean free paths, energy dispersion, and screening of the IR laser field on the surface. Supported by the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy under Grant No. DE-FG02-86ER13491 and NSF Grant PHY-1068752.

  12. Reliable and Repeatable Characterization of Optical Streak Cameras

    SciTech Connect

    Michael Charest Jr., Peter Torres III, Christopher Silbernagel, and Daniel Kalantar

    2008-10-31

    Optical streak cameras are used as primary diagnostics for a wide range of physics and laser experiments at facilities such as the National Ignition Facility (NIF). To meet the strict accuracy requirements needed for these experiments, the systematic nonlinearities of the streak cameras (attributed to nonlinearities in the optical and electrical components that make up the streak camera system) must be characterized. In some cases the characterization information is used as a guide to help determine how experiment data should be taken. In other cases, the characterization data are applied to the raw data images to correct for the nonlinearities. In order to characterize an optical streak camera, a specific set of data is collected, where the response to defined inputs are recorded. A set of analysis software routines has been developed to extract information such as spatial resolution, dynamic range, and temporal resolution from this data set. The routines are highly automated, requiring very little user input and thus provide very reliable and repeatable results that are not subject to interpretation. An emphasis on quality control has been placed on these routines due to the high importance of the camera characterization information.

  13. Reliable and Repeatable Characterication of Optical Streak Cameras

    SciTech Connect

    Kalantar, D; Charest, M; Torres III, P; Charest, M

    2008-05-06

    Optical streak cameras are used as primary diagnostics for a wide range of physics and laser experiments at facilities such as the National Ignition Facility (NIF). To meet the strict accuracy requirements needed for these experiments, the systematic nonlinearities of the streak cameras (attributed to nonlinearities in the optical and electrical components that make up the streak camera system) must be characterized. In some cases the characterization information is used as a guide to help determine how experiment data should be taken. In other cases, the characterization data are applied to the raw data images to correct for the nonlinearities. In order to characterize an optical streak camera, a specific set of data is collected, where the response to defined inputs are recorded. A set of analysis software routines has been developed to extract information such as spatial resolution, dynamic range, and temporal resolution from this data set. The routines are highly automated, requiring very little user input and thus provide very reliable and repeatable results that are not subject to interpretation. An emphasis on quality control has been placed on these routines due to the high importance of the camera characterization information.

  14. Viruses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Lytic bacteriophages, viruses which infect and lyse bacterial cells, can provide a natural method to reduce bacterial pathogens on produce commodities. The use of multi-phage cocktails is most likely to be effective against bacterial pathogens on produce commodities, and minimize the development of...

  15. Hitting is contagious in baseball: evidence from long hitting streaks.

    PubMed

    Bock, Joel R; Maewal, Akhilesh; Gough, David A

    2012-01-01

    Data analysis is used to test the hypothesis that "hitting is contagious". A statistical model is described to study the effect of a hot hitter upon his teammates' batting during a consecutive game hitting streak. Box score data for entire seasons comprising [Formula: see text] streaks of length [Formula: see text] games, including a total [Formula: see text] observations were compiled. Treatment and control sample groups ([Formula: see text]) were constructed from core lineups of players on the streaking batter's team. The percentile method bootstrap was used to calculate [Formula: see text] confidence intervals for statistics representing differences in the mean distributions of two batting statistics between groups. Batters in the treatment group (hot streak active) showed statistically significant improvements in hitting performance, as compared against the control. Mean [Formula: see text] for the treatment group was found to be [Formula: see text] to [Formula: see text] percentage points higher during hot streaks (mean difference increased [Formula: see text] points), while the batting heat index [Formula: see text] introduced here was observed to increase by [Formula: see text] points. For each performance statistic, the null hypothesis was rejected at the [Formula: see text] significance level. We conclude that the evidence suggests the potential existence of a "statistical contagion effect". Psychological mechanisms essential to the empirical results are suggested, as several studies from the scientific literature lend credence to contagious phenomena in sports. Causal inference from these results is difficult, but we suggest and discuss several latent variables that may contribute to the observed results, and offer possible directions for future research. PMID:23251507

  16. Monitoring Nonadiabatic Electron-Nuclear Dynamics in Molecules by Attosecond Streaking of Photoelectrons.

    PubMed

    Kowalewski, Markus; Bennett, Kochise; Rouxel, Jérémy R; Mukamel, Shaul

    2016-07-22

    Streaking of photoelectrons has long been used for the temporal characterization of attosecond extreme ultraviolet pulses. When the time-resolved photoelectrons originate from a coherent superposition of electronic states, they carry additional phase information, which can be retrieved by the streaking technique. In this contribution we extend the streaking formalism to include coupled electron and nuclear dynamics in molecules as well as initial coherences. We demonstrate how streaked photoelectrons offer a novel tool for monitoring nonadiabatic dynamics as it occurs in the vicinity of conical intersections and avoided crossings. Streaking can provide high time resolution direct signatures of electronic coherences, which affect many primary photochemical and biological events. PMID:27494470

  17. Monitoring Nonadiabatic Electron-Nuclear Dynamics in Molecules by Attosecond Streaking of Photoelectrons

    NASA Astrophysics Data System (ADS)

    Kowalewski, Markus; Bennett, Kochise; Rouxel, Jérémy R.; Mukamel, Shaul

    2016-07-01

    Streaking of photoelectrons has long been used for the temporal characterization of attosecond extreme ultraviolet pulses. When the time-resolved photoelectrons originate from a coherent superposition of electronic states, they carry additional phase information, which can be retrieved by the streaking technique. In this contribution we extend the streaking formalism to include coupled electron and nuclear dynamics in molecules as well as initial coherences. We demonstrate how streaked photoelectrons offer a novel tool for monitoring nonadiabatic dynamics as it occurs in the vicinity of conical intersections and avoided crossings. Streaking can provide high time resolution direct signatures of electronic coherences, which affect many primary photochemical and biological events.

  18. Corn rootworms and Bt resistance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn rootworms have been a major pest of corn for many years. As their name suggests, corn rootworms damage corn plants by feeding on the roots. Western and northern corn rootworms have overcome practices farmers use to keep their population numbers down, such as insecticides and crop rotation. Cor...

  19. Genetic analysis and molecular mapping of QTLs for resistance to rice black-streaked dwarf disease in rice

    PubMed Central

    Zhou, Tong; Du, Linlin; Wang, Lijiao; Wang, Ying; Gao, Cunyi; Lan, Ying; Sun, Feng; Fan, Yongjian; Wang, Guoliang; Zhou, Yijun

    2015-01-01

    Rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (RBSDV), is transmitted by small brown planthoppers (Laodelphax striatellus Fallén, SBPH) and causes severe yield loss in epidemic years in China and other East Asian countries. Breeding for resistance to RBSDV is a promising strategy to control the disease. We identified Tetep that showed resistance to RBSDV using a field test and artificial inoculation test. An evaluation of the resistance mechanism revealed that Tetep was resistant to RBSDV but not to SBPH. Genetic analysis showed that the resistance of Tetep to RBSDV was controlled by quantitative trait loci (QTLs). Three new QTLs for RBSDV resistance were identified in this study, i.e., qRBSDV-3, qRBSDV-10 and qRBSDV-11. The LOD scores of qRBSDV-3, qRBSDV-10 and qRBSDV-11 were 4.07, 2.24 and 2.21, accounting for 17.5%, 0.3% and 12.4% of the total phenotypic variation, respectively. All the resistance loci identified in this study were associated with virus resistance genes. The alleles for enhancing resistance on chromosomes 3 and 11 originated from Tetep, whereas the other allele on chromosome 10 originated from a susceptible parent. The identified new resistance QTLs in this study are useful resources for efficiently breeding resistant rice cultivars to RBSDV. PMID:26198760

  20. Identifying resistance in corn to southwestern corn borer, fall armyworm, and corn earworm

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Southwestern corn borer, Diatraea grandiosella Dyar; fall armyworm, Spodoptera frugiperda (J.E. Smith); and corn earworm, Helicoverpa zea Boddie, are major insect pests of corn, Zea mays L., in the southern United States. Corn germplasm with resistance to leaf feeding by southwestern corn borer a...

  1. Development of streak camera with anisotropic focusing electron optical system

    NASA Astrophysics Data System (ADS)

    Tian, J.; Ding, Y.; Cao, X.; Liu, S.; Xu, X.; Hu, X.; Wen, W.; Wang, J.; Wang, C.; Liu, H.; Dong, G.; Zhang, T.; Lu, Y.; Wang, Xi.; Liu, J.

    2013-05-01

    In this paper, the anisotropic focusing technique is used to make a novel streak tube. The salient features are the introduction of both temporally focusing electrodes and spatially focusing electric quadrupole lens. The simulation showed that physical temporal dispersion of 0.38 ps and edge spatial resolution of 56 lp/mm can be achieved. The Nd:YLF 8ps pulse laser was used to calibrate the performance index of streak camera. The static and dynamic spatial resolutions are 35 lp/mm and 25 lp/mm respectively. The dynamic range more than 950:1 and time resolution 8ps can be reached. Furthermore, the magnifications in slit and scanning direction can be adjusted respectively, so it is very convenient to select amplification needed when it is coupled with KB microscope.

  2. Megahertz streak-mode Fourier domain optical coherence tomography

    PubMed Central

    Wang, Rui; Yun, Julie X.; Yuan, Xiaocong; Goodwin, Richard; Markwald, Roger R.; Gao, Bruce Z.

    2011-01-01

    Here we present an ultrahigh-speed Fourier-domain optical coherence tomography (OCT) that records the OCT spectrum in streak mode with a high-speed area scan camera, which allows higher OCT imaging speed than can be achieved with a line-scan camera. Unlike parallel OCT techniques that also use area scan cameras, the conventional single-mode fiber-based point-scanning mechanism is retained to provide a confocal gate that rejects multiply scattered photons from the sample. When using a 1000 Hz resonant scanner as the streak scanner, 1,016,000 A-scans have been obtained in 1 s. This method's effectiveness has been demonstrated by recording in vivo OCT-image sequences of embryonic chick hearts at 1000 frames/s. In addition, 2-megahertz OCT data have been obtained with another high speed camera. PMID:21721817

  3. Streak cameras for soft x-ray and optical radiation

    SciTech Connect

    Medecki, H.

    1983-09-01

    The principal component of a streak camera is the image converter tube. A slit-shaped photocathode transforms the radiation into a proportional emission of electrons. An electron - optics arrangement accelerates the electrons and projects them into a phosphor screen creating the image of the slit. A pair of deflection plates deflects the electronic beam along a direction perpendicular to the main dimension of the slit. Different portions of the phosphor screen show the instantaneous image of the slit with brightness proportional to the number of emitted electrons and, consequently, to the intensity of the radiation. For our x-ray streak cameras, we use the RCA C73435A image conventer tube intended for the measurement of the radiation of light and modified to have an x-ray sensitive photocathode. Practical considerations lead to the use of transparent rather than reflecting photocathodes. Several of these camera tubes are briefly described.

  4. rf streak camera based ultrafast relativistic electron diffraction.

    PubMed

    Musumeci, P; Moody, J T; Scoby, C M; Gutierrez, M S; Tran, T

    2009-01-01

    We theoretically and experimentally investigate the possibility of using a rf streak camera to time resolve in a single shot structural changes at the sub-100 fs time scale via relativistic electron diffraction. We experimentally tested this novel concept at the UCLA Pegasus rf photoinjector. Time-resolved diffraction patterns from thin Al foil are recorded. Averaging over 50 shots is required in order to get statistics sufficient to uncover a variation in time of the diffraction patterns. In the absence of an external pump laser, this is explained as due to the energy chirp on the beam out of the electron gun. With further improvements to the electron source, rf streak camera based ultrafast electron diffraction has the potential to yield truly single shot measurements of ultrafast processes. PMID:19191429

  5. Angioid streaks - a rare cause of neovascular glaucoma. Case report.

    PubMed

    Ungureanu, E; Geamanu, A; Careba, I; Grecescu, M; Gradinaru, S

    2014-01-01

    Rationale. Neovascular glaucoma is the type of glaucoma most refractory to treatment. The most frequent causes are those associated with retinal hypoxia, such as proliferative diabetic retinopathy, central retinal vein occlusion, branch retinal vein occlusion, central retinal arterial occlusion, ischemic ocular syndrome etc. Rare causes of neovascular glaucoma are multiple and are due to VEGF synthesis associated with chorioretinal inflammations or degenerations. We present a case with neovascular glaucoma associated with an extremely rare cause, angioid streaks Objective. The objective of our prsentation was to asses efficacy of the 5-FU associated trabeculectomy following bevacizumab intravitreal administration Methods and results. Case report of a 48 years old female patient which presented at the emergency room with painful red left eye. At presentation best corrected left eye visual acuity was 1/10, intraocular pressure was 36 mm Hg. Examination established the diagnosis of Neovascular glaucoma associated with angioid streaks. After intravenous Manitol, oral Acetazolamide and topical treatment with fixed combination timolol-brinzolamide, topical steroid and mydriatic intraocular pressure decreased. Intravitreal bevacizumab injection was performed, followed after 3 weeks by trabeculectomy. Discussion. Angioid streaks are an extremely rare cause of neovascular glaucoma. The treatment is similar to the treatment for other causes of neovascular glaucoma. PMID:27057253

  6. Automatic coil selection for streak artifact reduction in radial MRI.

    PubMed

    Xue, Yiqun; Yu, Jiangsheng; Kang, Hyun Seon; Englander, Sarah; Rosen, Mark A; Song, Hee Kwon

    2012-02-01

    In radial MR imaging, streaking artifacts contaminating the entire field of view can arise from regions at the outer edges of the prescribed field of view. This can occur even when the Nyquist criterion is satisfied within the desired field of view. These artifacts become exacerbated when parts of the object lie in the superior/inferior regions of the scanner where the gradient strengths become weakened. When multiple coil arrays are used for signal reception, coils at the outer edges can be disabled before data acquisition to reduce the artifact levels. However, as the weakened gradient strengths near the edges often distort the object, causing the signal to become highly concentrated into a small region, the streaks are often not completely removed. Data from certain coils can also be excluded during reconstruction by visually inspecting the individual coil images, but this is impractical for routine use. In this work, a postprocessing method is proposed to automatically identify those coils whose images contain high levels of streaking for subsequent exclusion during reconstruction. The proposed method was demonstrated in vivo dynamic contrast enhanced MRI datasets acquired using a three-dimensional hybrid radial sequence. The results demonstrate that the proposed strategy substantially improves the image quality and show excellent agreement with images reconstructed with manually determined coil selection. PMID:21656562

  7. Streak artifact reduction in cardiac cone beam CT

    NASA Astrophysics Data System (ADS)

    Shechter, Gilad; Naveh, Galit; Lessick, Jonathan; Altman, Ami

    2005-04-01

    Cone beam reconstructed cardiac CT images suffer from characteristic streak artifacts that affect the quality of coronary artery imaging. These artifacts arise from inhomogeneous distribution of noise. While in non-tagged reconstruction inhomogeneity of noise distribution is mainly due to anisotropy of the attenuation of the scanned object (e.g. shoulders), in cardiac imaging it is largely influenced by the non-uniform distribution of the acquired data used for reconstructing the heart at a given phase. We use a cardiac adaptive filter to reduce these streaks. In difference to previous methods of adaptive filtering that locally smooth data points on the basis of their attenuation values, our filter is applied as a function of the noise distribution of the data as it is used in the phase selective reconstruction. We have reconstructed trans-axial images without adaptive filtering, with a regular adaptive filter and with the cardiac adaptive filter. With the cardiac adaptive filter significant reduction of streaks is achieved, and thus image quality is improved. The coronary vessel is much more pronounced in the cardiac adaptive filtered images, in slab MIP the main coronary artery branches are more visible, and non-calcified plaque is better differentiated from vessel wall. This improvement is accomplished without altering significantly the border definition of calcified plaques.

  8. Angioid streaks - a rare cause of neovascular glaucoma. Case report.

    PubMed Central

    Ungureanu, E; Geamanu, A; Careba, I; Grecescu, M; Gradinaru, S

    2014-01-01

    Rationale. Neovascular glaucoma is the type of glaucoma most refractory to treatment. The most frequent causes are those associated with retinal hypoxia, such as proliferative diabetic retinopathy, central retinal vein occlusion, branch retinal vein occlusion, central retinal arterial occlusion, ischemic ocular syndrome etc. Rare causes of neovascular glaucoma are multiple and are due to VEGF synthesis associated with chorioretinal inflammations or degenerations. We present a case with neovascular glaucoma associated with an extremely rare cause, angioid streaks Objective. The objective of our prsentation was to asses efficacy of the 5-FU associated trabeculectomy following bevacizumab intravitreal administration Methods and results. Case report of a 48 years old female patient which presented at the emergency room with painful red left eye. At presentation best corrected left eye visual acuity was 1/10, intraocular pressure was 36 mm Hg. Examination established the diagnosis of Neovascular glaucoma associated with angioid streaks. After intravenous Manitol, oral Acetazolamide and topical treatment with fixed combination timolol-brinzolamide, topical steroid and mydriatic intraocular pressure decreased. Intravitreal bevacizumab injection was performed, followed after 3 weeks by trabeculectomy. Discussion. Angioid streaks are an extremely rare cause of neovascular glaucoma. The treatment is similar to the treatment for other causes of neovascular glaucoma PMID:27057253

  9. Infrared imagery of streak formation in a breaking wave

    NASA Astrophysics Data System (ADS)

    Handler, Robert A.; Savelyev, Ivan; Lindsey, Michael

    2012-12-01

    High resolution infrared imagery of breaking waves in a wave-tank free of wind shear or current reveals the production of a "streaky," quasi-periodic thermal pattern produced during the breaking process. The streaks, or elongated patterns of warm and cold fluid, are found to form only when surface turbulence is present before wave breaking occurs. This suggests that wave-turbulence interaction is one mechanism that can lead to streak formation in breaking wave systems. More specifically, the streaky structures observed in these experiments may be caused by an intense, rapid tilting, and stretching of pre-existing vertical vorticity by the Stokes drift generated at or near the breaking wave crests, thereby generating a coherent system of counter-rotating vortices. We attempt to relate our observations to the recent theory of Teixeira and Belcher [J. Fluid Mech. 458, 229-267 (2002), 10.1017/S0022112002007838]. Some properties of the streaks, such as the dependence of their lifetimes and spanwise scale on wave amplitude, are presented.

  10. Design of microcontroller based system for automation of streak camera

    SciTech Connect

    Joshi, M. J.; Upadhyay, J.; Deshpande, P. P.; Sharma, M. L.; Navathe, C. P.

    2010-08-15

    A microcontroller based system has been developed for automation of the S-20 optical streak camera, which is used as a diagnostic tool to measure ultrafast light phenomenon. An 8 bit MCS family microcontroller is employed to generate all control signals for the streak camera. All biasing voltages required for various electrodes of the tubes are generated using dc-to-dc converters. A high voltage ramp signal is generated through a step generator unit followed by an integrator circuit and is applied to the camera's deflecting plates. The slope of the ramp can be changed by varying values of the capacitor and inductor. A programmable digital delay generator has been developed for synchronization of ramp signal with the optical signal. An independent hardwired interlock circuit has been developed for machine safety. A LABVIEW based graphical user interface has been developed which enables the user to program the settings of the camera and capture the image. The image is displayed with intensity profiles along horizontal and vertical axes. The streak camera was calibrated using nanosecond and femtosecond lasers.

  11. Resolution limitations and optimization of the LLNL streak camera focus

    SciTech Connect

    Lerche, R.A.; Griffith, R.L.

    1987-09-01

    The RCA C73435 image tube is biased at voltages far from its original design in the LLNL ultrafast (10 ps) streak camera. Its output resolution at streak camera operating potentials has been measured as a function of input slit width, incident-light wavelength, and focus-grid voltage. The temporal resolution is insensitive to focus-grid voltage for a narrow (100 ..mu..m) input slit, but is very sensitive to focus-grid voltage for a wide (2 mm) input slit. At the optimum wide-slit focus voltage, temporal resolution is insensitive to slit width. Spatial resolution is nearly independent of focus-grid voltage for values that give good temporal resolution. Both temporal and spatial resolution depend on the incident-light wavelength. Data for 1.06-..mu..m light show significantly better focusing than for 0.53-..mu..m light. Streak camera operation is simulated with a computer program that calculates photoelectron trajectories. Electron ray tracing describes all of the observed effects of slit width, incident-light wavelength, and focus-grid voltage on output resolution. 7 refs.

  12. Slope streaks on Mars: A new “wet” mechanism

    NASA Astrophysics Data System (ADS)

    Kreslavsky, Mikhail A.; Head, James W.

    2009-06-01

    Slope steaks are one of the most intriguing modern phenomena observed on Mars. They have been mostly interpreted as some specific type of granular flow. We propose another mechanism for slope streak formation on Mars. It involves natural seasonal formation of a modest amount of highly concentrated chloride brines within a seasonal thermal skin, and runaway propagation of percolation fronts. Given the current state of knowledge of temperature regimes and the composition and structure of the surface layer in the slope streak regions, this mechanism is consistent with the observational constraints; it requires an assumption that a significant part of the observed chlorine to be in form of calcium and ferric chloride, and a small part of the observed hydrogen to be in form of water ice. This "wet" mechanism has a number of appealing advantages in comparison to the widely accepted "dry" granular flow mechanism. Potential tests for the "wet" mechanism include better modeling of the temperature regime and observations of the seasonality of streak formation.

  13. Design of microcontroller based system for automation of streak camera

    NASA Astrophysics Data System (ADS)

    Joshi, M. J.; Upadhyay, J.; Deshpande, P. P.; Sharma, M. L.; Navathe, C. P.

    2010-08-01

    A microcontroller based system has been developed for automation of the S-20 optical streak camera, which is used as a diagnostic tool to measure ultrafast light phenomenon. An 8 bit MCS family microcontroller is employed to generate all control signals for the streak camera. All biasing voltages required for various electrodes of the tubes are generated using dc-to-dc converters. A high voltage ramp signal is generated through a step generator unit followed by an integrator circuit and is applied to the camera's deflecting plates. The slope of the ramp can be changed by varying values of the capacitor and inductor. A programmable digital delay generator has been developed for synchronization of ramp signal with the optical signal. An independent hardwired interlock circuit has been developed for machine safety. A LABVIEW based graphical user interface has been developed which enables the user to program the settings of the camera and capture the image. The image is displayed with intensity profiles along horizontal and vertical axes. The streak camera was calibrated using nanosecond and femtosecond lasers.

  14. Stabilization of boundary layer streaks by plasma actuators

    NASA Astrophysics Data System (ADS)

    Riherd, Mark; Roy, Subrata

    2014-03-01

    A flow's transition from laminar to turbulent leads to increased levels of skin friction. In recent years, dielectric barrier discharge actuators have been shown to be able to delay the onset of turbulence in boundary layers. While the laminar to turbulent transition process can be initiated by several different instability mechanisms, so far, only stabilization of the Tollmien-Schlichting path to transition has received significant attention, leaving the stabilization of other transition paths using these actuators less explored. To fill that void, a bi-global stability analysis is used here to examine the stabilization of boundary layer streaks in a laminar boundary layer. These streaks, which are important to both transient and by-pass instability mechanisms, are damped by the addition of a flow-wise oriented plasma body force to the boundary layer. Depending on the magnitude of the plasma actuation, this damping can be up to 25% of the perturbation's kinetic energy. The damping mechanism appears to be due to highly localized effects in the immediate vicinity of the body force, and when examined using a linearized Reynolds-averaged Navier-Stokes energy balance, indicate negative production of the perturbation's kinetic energy. Parametric studies of the stabilization have also been performed, varying the magnitude of the plasma actuator's body force and the spanwise wavenumber of the actuation. Based on these parametric studies, the damping of the boundary layer streaks appears to be linear with respect to the total amount of body force applied to the flow.

  15. Analysis of extraembryonic mesodermal structure formation in the absence of morphological primitive streak.

    PubMed

    Jin, Jiu-Zhen; Zhu, Yuanqi; Warner, Dennis; Ding, Jixiang

    2016-08-01

    During mouse gastrulation, the primitive streak is formed on the posterior side of the embryo. Cells migrate out of the primitive streak to form the future mesoderm and endoderm. Fate mapping studies revealed a group of cell migrate through the proximal end of the primitive streak and give rise to the extraembryonic mesoderm tissues such as the yolk sac blood islands and allantois. However, it is not clear whether the formation of a morphological primitive streak is required for the development of these extraembryonic mesodermal tissues. Loss of the Cripto gene in mice dramatically reduces, but does not completely abolish, Nodal activity leading to the absence of a morphological primitive streak. However, embryonic erythrocytes are still formed and assembled into the blood islands. In addition, Cripto mutant embryos form allantoic buds. However, Drap1 mutant embryos have excessive Nodal activity in the epiblast cells before gastrulation and form an expanded primitive streak, but no yolk sac blood islands or allantoic bud formation. Lefty2 embryos also have elevated levels of Nodal activity in the primitive streak during gastrulation, and undergo normal blood island and allantois formation. We therefore speculate that low level of Nodal activity disrupts the formation of morphological primitive streak on the posterior side, but still allows the formation of primitive streak cells on the proximal side, which give rise to the extraembryonic mesodermal tissues formation. Excessive Nodal activity in the epiblast at pre-gastrulation stage, but not in the primitive streak cells during gastrulation, disrupts extraembryonic mesoderm development. PMID:27273137

  16. Complete Genome Sequence of the Alfalfa latent virus.

    PubMed

    Nemchinov, Lev G; Shao, Jonathan; Postnikova, Olga A

    2015-01-01

    The first complete genome sequence of the Alfalfa latent carlavirus (ALV) was obtained by primer walking and Illumina RNA sequencing. The virus differs substantially from the Czech ALV isolate and the Pea streak virus isolate from Wisconsin. The absence of a clear nucleic acid-binding protein indicates ALV divergence from other carlaviruses. PMID:25883281

  17. Complete Genome Sequence of the Alfalfa latent virus

    PubMed Central

    Shao, Jonathan; Postnikova, Olga A.

    2015-01-01

    The first complete genome sequence of the Alfalfa latent carlavirus (ALV) was obtained by primer walking and Illumina RNA sequencing. The virus differs substantially from the Czech ALV isolate and the Pea streak virus isolate from Wisconsin. The absence of a clear nucleic acid-binding protein indicates ALV divergence from other carlaviruses. PMID:25883281

  18. The Nucleotide Sequence of Blackberry chlorotic ringspot and Strawberry necrotic shock viruses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Small fruit crops were thought to be infected with distinct stains of Tobacco streak virus (TSV) for more than 50 years. Two novel ilarviruses, Blackberry chlorotic ringspot virus (BCRV) and Strawberry necrotic shock virus (SNSV) were found to infect small fruit crops. Using TSV specific molecular ...

  19. Corn blight watch experiment

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The corn blight problem is briefly described how the experiment was organized and conducted, the effect of the blight on the 1971 crop, and some conclusions that may be drawn as a result of the experiment. The information is based on preliminary reports of the Corn Blight Watch Steering Committee and incorporates much illustrative material conceived at Purdue University.

  20. PRODUCING HIGH CORN YIELDS.

    ERIC Educational Resources Information Center

    Illinois Univ., Urbana. Coll. of Agriculture.

    RESOURCE MATERIAL ON CORN PRODUCTION FOR HIGH SCHOOL VOCATIONAL AGRICULTURE AND ADULT FARMER CLASSES WAS DESIGNED BY A STATE LEVEL GROUP OF SUBJECT MATTER SPECIALISTS, TEACHER EDUCATORS, SUPERVISORS, AND TEACHERS TO HELP SOLVE PROBLEMS THAT CONFRONT CORN PRODUCERS AT PLANTING TIME. THE SUBJECT MATTER CONCERNS PLANTING TIME, DEPTH, ROW WIDTH,…

  1. On the nature and visibility of crater-associated streaks on Mars

    NASA Technical Reports Server (NTRS)

    Veverka, J.; Thomas, P.; Sagan, C.

    1978-01-01

    The paper considers Mariner 9 and Viking data that contradict Kuzmin's (1975) hypothesis that all crater-associated wind streaks on Mars are depositional and consist of unresolved barchan-like dunes. According to Kuzmin's hypothesis, any streak can appear either bright or dark relative to its surroundings depending on the sun's position. The spacecraft images, however, show examples of dark and light streaks visible at the same azimuth angle of the sun. Evidence that bright and dark streaks differ both in morphology and in character is considered. It is suggested that the common ragged dark streaks are probably erosion scars while most bright streaks probably represent accumulations of bright dust-storm fallout.

  2. Hot streaks and phantom cooling in a turbine rotor passage. II - Combined effects and analytical modelling

    NASA Technical Reports Server (NTRS)

    Roback, Richard J.; Dring, Robert P.

    1992-01-01

    Experimental documentation and analytical correlations demonstrating the effects of hot streak accumulation and phantom cooling on turbine rotor airfoil surface temperature are presented. Results are shown which quantify the impact of a nonuniform temperature profile at the entrance of a turbine due to combustor-generated hot and cold streaks, and cooling air discharged from the trailing edge of the upstream stator. Experimental results are shown for a range of controlling variables to identify where streak accumulation and phantom cooling were most likely to be strongest. These variables include streak-to-free stream density ratio, streak injection location, and coolant-to-free stream density and velocity ratios. Experimental results are shown for the combined effects of hot streak and stator coolant on the adiabatic recovery temperature of the rotor.

  3. Surface properties of the Pettit wind streak on Mars Implications for sediment transport

    NASA Astrophysics Data System (ADS)

    Zimbelman, J. R.

    1986-04-01

    Satellite imagery of the Pettit streak on Amazonis Planitia are discussed in terms of the details of wind streaks and their associated sediments. Increasingly more detailed blow-ups of the available imagery demonstrate that the Pettit streak holds both a Type I bright streak (at the crater rim) and a Type II dark streak beginning at the dark patch within the crater. The lowest albedos measured, 0.20-0.22, are associated with the highest thermal inertia, indicative of grain diameters similar to medium sand, i.e., ranging from 250-350 microns. The brightest portions of the streak have albedos over 0.26 and a low thermal inertia, in the range 3-4, which implies the presence of fine-grained sand, diameters from 50-100 microns. The particle grains are less than 50 microns diameter in the surrounding plains, which have an approximately uniform albedo (0.27) and a thermal inertia of 2.5, characteristics typical of silt or clay. Current streak models describe the dark streak well, but do not account for the bright streak, which may be optically thick patches of very fine dust.

  4. Defrosting Polar Dunes--Dark Spots and Wind Streaks

    NASA Technical Reports Server (NTRS)

    1999-01-01

    The first time that the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC)team saw dark spots on defrosting dune surfaces was in August and September of 1998. At that time, it was the north polar seasonal frost cap that was subliming away (more recent images from 1999 have shown the south polar frosts). This picture (above) shows a small portion of the giant dune field that surrounds the north polar region, as it appeared on August 23, 1998. At the time, it was early northern spring and the dunes were still covered with winter frost.

    Dark spots had appeared on the north polar dunes, and many of them exhibited a radial or semi-radial pattern of dark streaks and streamers. At first, there was speculation that the streaks indicated that the defrosting process might somehow involve explosions! The dark spots seemed to resemble small craters with dark, radial ejecta. It seemed possible that frozen carbon dioxide trapped beneath water ice might somehow heat up, turn to gas, expand, and then 'explode' in either a small blast or at least a 'puff' of air similar to that which comes from the blowhole of a surfacing whale or seal.

    The image shown here changed the earlier impression. The dark spots and streaks do not result from explosions. The spots--though not well understood--represent the earliest stages of defrosting on the sand dunes. The streaks, instead of being caused by small explosions, are instead the result of wind. In this picture, the fine, dark streaks show essentially identical orientations from spot to spot (e.g., compare the spots seen in boxes (a) and (b)). Each ray of dark material must result from wind blowing from a particular direction--for example, all of the spots in this picture exhibit a ray that points toward the upper left corner of the image, and each of these rays indicates the same wind regime. Each spot also has a ray pointing toward the lower right and top/upper-right. These, too, must indicate periods when the wind was strong

  5. Streaked radiography measurements of convergent ablator performance (invited)

    SciTech Connect

    Hicks, D. G.; Spears, B. K.; Braun, D. G.; Olson, R. E.; Sorce, C. M.; Celliers, P. M.; Collins, G. W.; Landen, O. L.

    2010-10-15

    The velocity and remaining ablator mass of an imploding capsule are critical metrics for assessing the progress toward ignition of an inertially confined fusion experiment. These and other ablator rocket parameters have been measured using a single streaked x-ray radiograph. A regularization technique has been used to determine the ablator density profile {rho}(r) at each time step; moments of {rho}(r) then provide the areal density, average radius, and mass of the unablated, or remaining, ablator material, with the velocity determined from the time derivative of the average radius. The technique has been implemented on experiments at the OMEGA laser facility.

  6. Microprocessor-controlled, wide-range streak camera

    SciTech Connect

    Amy E. Lewis, Craig Hollabaugh

    2006-09-01

    Bechtel Nevada/NSTec recently announced deployment of their fifth generation streak camera. This camera incorporates many advanced features beyond those currently available for streak cameras. The arc-resistant driver includes a trigger lockout mechanism, actively monitors input trigger levels, and incorporates a high-voltage fault interrupter for user safety and tube protection. The camera is completely modular and may deflect over a variable full-sweep time of 15 nanoseconds to 500 microseconds. The camera design is compatible with both large- and small-format commercial tubes from several vendors. The embedded microprocessor offers Ethernet connectivity, and XML [extensible markup language]-based configuration management with non-volatile parameter storage using flash-based storage media. The camera’s user interface is platform-independent (Microsoft Windows, Unix, Linux, Macintosh OSX) and is accessible using an AJAX [asynchronous Javascript and XML]-equipped modem browser, such as Internet Explorer 6, Firefox, or Safari. User interface operation requires no installation of client software or browser plug-in technology. Automation software can also access the camera configuration and control using HTTP [hypertext transfer protocol]. The software architecture supports multiple-simultaneous clients, multiple cameras, and multiple module access with a standard browser. The entire user interface can be customized.

  7. Range accuracy analysis of streak tube imaging lidar systems

    NASA Astrophysics Data System (ADS)

    Ye, Guangchao; Fan, Rongwei; Chen, Zhaodong; Yuan, Wei; Chen, Deying; He, Ping

    2016-02-01

    Streak tube imaging lidar (STIL) is an active imaging system that has a high range accuracy and a wide range gate with the use of a pulsed laser transmitter and streak tube receiver to produce 3D range images. This work investigates the range accuracy performance of STIL systems based on a peak detection algorithm, taking into account the effects of blurring of the image. A theoretical model of the time-resolved signal distribution, including the static blurring width in addition to the laser pulse width, is presented, resulting in a modified range accuracy analysis. The model indicates that the static blurring width has a significant effect on the range accuracy, which is validated by both the simulation and experimental results. By using the optimal static blurring width, the range accuracies are enhanced in both indoor and outdoor experiments, with a stand-off distance of 10 m and 1700 m, respectively, and corresponding, best range errors of 0.06 m and 0.25 m were achieved in a daylight environment.

  8. [Further development of a streak retinoscope with calibrated collimator].

    PubMed

    Rohrschneider, K; Koch, H R

    1992-08-01

    Development and rationale of a new streak retinoscope have been discussed in an earlier paper. In contrast to classical retinoscopy this retinoscope determines the refraction by forming the narrowest streak in the test person's pupil with a calibrated collimator. In addition to the first prototype the extension of the lamp according to variation of the refraction is measured electronically and the data are transposed into a computer. This allows to freely choose a working distance in a range from about 30 to 120 cm and therefore it is possible to examine restless patients. Accuracy of the measurement was less than or equal to 0.25 D for Ametropia from -0.5 to 5.0 D in a working distance of 30 cm or from -4.0 to -1.5 D in 100 cm. In most persons it is not necessary to use additional glasses during refraction by choosing the adequate distance. Therefore this method can help to refract kids and other persons which could not be refracted by use of glasses. In addition this method excludes the accommodation of the patient as apposed to regular refractometers. PMID:1434382

  9. Kepler Corn Maze

    NASA Video Gallery

    The Dell'Osso Family Farm, located on the outskirts of Lathrop, California held the grand opening of their corn maze that was designed with a NASA theme. The maze is part of a nation-wide group of ...

  10. TQM at Corning.

    PubMed

    Houghton, J

    1992-01-01

    Houghton has been with Corning for 30 years, up through the ranks. Now, as chairman and CEO, his individual leadership stamp is TQM. It's a major turn-around story with Total Quality at its center. PMID:10117839

  11. Colony establishment and maintenance of the eriophyid wheat curl mite Aceria tosichella for controlled transmission studies on a new virus-like pathogen.

    PubMed

    Skare, J M; Wijkamp, I; Rezende, J; Michels, G; Rush, C; Scholthof, K-B G; Scholthof, H B

    2003-03-01

    High plains disease (HPD) is of serious economic concern for wheat and corn production, but little is known about the virus-like causal agent. In the field, HPD is often associated with Wheat streak mosaic virus (WSMV) and both pathogens are transmitted by the same eriophyid wheat curl mite, Aceria tosichella Keifer. The objective of this study was to develop methods for establishing and maintaining HPD-transmitting wheat curl mite colonies for their use in studies on HPD. Towards this goal, mite colonies from a mixed infection source were separated into colonies either (i). not viruliferous; (ii). only transmitting WSMV; or (iii). only transmitting HPD. Maintenance of these colonies required strictly separated incubator facilities and adaptation of mite-suitable transfer techniques to permit frequent passages of mites to healthy plants. The established colonies provided reliable sources of infective material to study the progression of HPD and/or WSMV in plants using sensitive immuno-detection assays. In conclusion, we have developed reliable methods with a poorly studied arthropod vector to examine the biology and properties of a new virus-like disease. PMID:12565164

  12. Argentina corn yield model

    NASA Technical Reports Server (NTRS)

    Callis, S. L.; Sakamoto, C.

    1984-01-01

    A model based on multiple regression was developed to estimate corn yields for the country of Argentina. A meteorological data set was obtained for the country by averaging data for stations within the corn-growing area. Predictor variables for the model were derived from monthly total precipitation, average monthly mean temperature, and average monthly maximum temperature. A trend variable was included for the years 1965 to 1980 since an increasing trend in yields due to technology was observed between these years.

  13. THEMIS-IR Emissivity Spectrum of a Large "Dark Streak" near Olympus Mons

    NASA Astrophysics Data System (ADS)

    Brumby, S. P.

    2004-03-01

    "Dark streaks" are unusual transient surface features found on Mars. We have obtained an infrared emissivity spectrum of a large dark streak on the north western edge of Olympus Mons, using imagery from the THEMIS instrument on the Mars Odyssey 2001 spacecraft.

  14. X-ray streak camera diagnostics of picosecond laser-plasma interactions

    SciTech Connect

    Cobble, J.A.; Fulton, R.D.; Jones, L.A.; Kyrala, G.A.; Schappert, G.T.; Taylor, A.J.; Wahlin, E.K.

    1992-05-01

    An x-ray streak camera is used to diagnose a laser-produced Al plasma with time resolution of {approximately}10 ps. A streak record of filtered emission and a time-integrated transmission grating spectrum reveal that the plasma radiation is dominated by emission from He- and H-like resonance lines. 11 refs.

  15. X-ray streak camera diagnostics of picosecond laser-plasma interactions

    SciTech Connect

    Cobble, J.A.; Fulton, R.D.; Jones, L.A.; Kyrala, G.A.; Schappert, G.T.; Taylor, A.J.; Wahlin, E.K.

    1992-01-01

    An x-ray streak camera is used to diagnose a laser-produced Al plasma with time resolution of {approximately}10 ps. A streak record of filtered emission and a time-integrated transmission grating spectrum reveal that the plasma radiation is dominated by emission from He- and H-like resonance lines. 11 refs.

  16. Averaging picosecond streak camera for time resolved x-ray and XUV spectroscopy of ultrashort plasmas

    NASA Astrophysics Data System (ADS)

    Maksimchuk, A.; Nantel, M.; Workman, J.; Umstadter, D.; Mourou, G.; Pikuz, S.

    1996-11-01

    We have developed an averaging picosecond x-ray streak camera that uses a DC-biased semi-insulating GaAs photoconductive switch as a generator of a high-voltage ramp for the deflection plates of the streak camera. The streak camera is operated at a sweep speed of up to 8 ps/mm, with a shot-to-shot jitter of 1 ps. The streak camera driven by this switch is absolutely synhronized with the femtosecond laser pulse and allows the direct accumulation of the x-ray temporal signals at a 10 Hz repetition rate. The streak camera has been used for the investigation of temporal behavior of keV x-ray lines and spectrally resolved XUV emission from solid target at the laser intensity of 10^17 W/cm^2. This work is supported by NSF under Grant STC PHY 8920108.

  17. Satellite Observations of Plume-like Streaks in a Cloud Field in Canada

    NASA Astrophysics Data System (ADS)

    Grasso, Lewis; Lindsey, Daniel T.; Seaman, Curtis J.; Stocks, Brian; Rabin, Robert M.

    2015-04-01

    On the afternoon of 28 October 2013, plume-like streaks were detected by geostationary and polar orbiting satellites over eastern Ontario, Canada. These streaks were characterized by enhanced reflectivity in the visible bands and warmer brightness temperatures at 3.9 µm. These streaks were part of a low-level liquid water cloud layer. Due to the similarity of the streaks to plume-like features in marine stratocumulus caused by smoke from the stacks of ships, so-called ship tracks, a local source of emitted aerosols was suspected and subsequently identified as the burning of logging residue. This event provides further support for the ability of locally enhanced aerosol loading to alter microphysical characteristics of clouds. Ship tracks, pollution plumes from industrial burning, and pyro-cumulus are known examples of this type of interaction. In addition, the plume-like streaks could be used indirectly to identify the location of the source of the emitted particles.

  18. Sub-picosecond streak camera measurements at LLNL: From IR to x-rays

    SciTech Connect

    Kuba, J; Shepherd, R; Booth, R; Steward, R; Lee, E W; Cross, R R; Springer, P T

    2003-12-21

    An ultra fast, sub-picosecond resolution streak camera has been recently developed at the LLNL. The camera is a versatile instrument with a wide operating wavelength range. The temporal resolution of up to 300 fs can be achieved, with routine operation at 500 fs. The streak camera has been operated in a wide wavelength range from IR to x-rays up to 2 keV. In this paper we briefly review the main design features that result in the unique properties of the streak camera and present its several scientific applications: (1) Streak camera characterization using a Michelson interferometer in visible range, (2) temporally resolved study of a transient x-ray laser at 14.7 nm, which enabled us to vary the x-ray laser pulse duration from {approx}2-6 ps by changing the pump laser parameters, and (3) an example of a time-resolved spectroscopy experiment with the streak camera.

  19. Betting Decision Under Break-Streak Pattern: Evidence from Casino Gaming.

    PubMed

    Fong, Lawrence Hoc Nang; So, Amy Siu Ian; Law, Rob

    2016-03-01

    Cognitive bias is prevalent among gamblers, especially those with gambling problems. Grounded in the heuristics theories, this study contributes to the literature by examining a cognitive bias triggered by the break streak pattern in the casino setting. We postulate that gamblers tend to bet on the latest outcome when there is a break-streak pattern. Moreover, three determinants of the betting decision under break-streak pattern, including the streak length of the alternative outcome, the frequency of the latest outcome, and gender, were identified and examined in this study. A non-participatory observational study was conducted among the Cussec gamblers in a casino in Macao. An analysis of 1229 bets confirms our postulation, particularly when the streak of the alternative outcome is long, the latest outcome is frequent, and the gamblers are females. The findings provide meaningful implications for casino management and public policymakers regarding the minimization of gambling harm. PMID:25967106

  20. Characterization of X-ray streak cameras for use on Nova

    SciTech Connect

    Kalantar, D.H.; Bell, P.M.; Costa, R.L.; Hammel, B.A.; Landen, O.L.; Orzechowski, T.J.; Hares, J.D.; Dymoke-Bradshaw, A.K.L.

    1996-09-01

    There are many different types of measurements that require a continuous time history of x-ray emission that can be provided with an x-ray streak camera. In order to properly analyze the images that are recorded with the x-ray streak cameras operated on Nova, it is important to account for the streak characterization of each camera. We have performed a number of calibrations of the streak cameras both on the bench as well as with Nova disk target shots where we use a time modulated laser intensity profile (self-beating of the laser) on the target to generate an x-ray comb. We have measured the streak camera sweep direction and spatial offset, curvature of the electron optics, sweep rate, and magnification and resolution of the electron optics.

  1. Characterization of X-ray streak cameras for use on Nova

    SciTech Connect

    Kalantar, D.H.; Bell, P.M.; Costa, R.L.; Hammel, B.A.; Landen, O.L.; Orzechowski, T.J.; Hares, J.D.; Dymoke-Bradshaw, A.K.L.

    1996-10-01

    There are many different types of measurements that require a continuous time history of x-ray emission that can be provided with an x-ray streak camera. In order to properly analyze the images that are recorded with the x-ray streak cameras operated on Nova, it is important to account for the streak characterization of each camera. We have performed a number of calibrations of the streak cameras both on the bench as well as with Nova disk target shots where we use a time modulated laser intensity profile (self-beating of the laser) an the target to generate an x-ray comb. We have measured the streak camera sweep direction and spatial offset, curvature of the electron optics, sweep rate, and magnification and resolution of the electron optics.

  2. 21 CFR 184.1321 - Corn gluten.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... glutelin. Corn gluten is a byproduct of the wet milling of corn for starch. The gluten fraction is washed... conversion of the starch in whole or various fractions of dry milled corn to corn syrups. (b) The...

  3. 21 CFR 184.1321 - Corn gluten.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... glutelin. Corn gluten is a byproduct of the wet milling of corn for starch. The gluten fraction is washed... conversion of the starch in whole or various fractions of dry milled corn to corn syrups. (b) The...

  4. 21 CFR 184.1321 - Corn gluten.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... glutelin. Corn gluten is a byproduct of the wet milling of corn for starch. The gluten fraction is washed... conversion of the starch in whole or various fractions of dry milled corn to corn syrups. (b) The...

  5. 21 CFR 184.1321 - Corn gluten.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... glutelin. Corn gluten is a byproduct of the wet milling of corn for starch. The gluten fraction is washed... conversion of the starch in whole or various fractions of dry milled corn to corn syrups. (b) The...

  6. On the convectively unstable nature of optimal streaks in boundary layers

    NASA Astrophysics Data System (ADS)

    Brandt, Luca; Cossu, Carlo; Chomaz, Jean-Marc; Huerre, Patrick; Henningson, Dan S.

    2003-06-01

    The objective of the study is to determine the absolute/convective nature of the secondary instability experienced by finite-amplitude streaks in the flat-plate boundary layer. A family of parallel streaky base flows is defined by extracting velocity profiles from direct numerical simulations of nonlinearly saturated optimal streaks. The computed impulse response of the streaky base flows is then determined as a function of streak amplitude and streamwise station. Both the temporal and spatio-temporal instability properties are directly retrieved from the impulse response wave packet, without solving the dispersion relation or applying the pinching point criterion in the complex wavenumber plane. The instability of optimal streaks is found to be unambiguously convective for all streak amplitudes and streamwise stations. It is more convective than the Blasius boundary layer in the absence of streaks; the trailing edge-velocity of a Tollmien Schlichting wave packet in the Blasius boundary layer is around 35% of the free-stream velocity, while that of the wave packet riding on the streaky base flow is around 70%. This is because the streak instability is primarily induced by the spanwise shear and the associated Reynolds stress production term is located further away from the wall, in a larger velocity region, than for the Tollmien Schlichting instability. The streak impulse response consists of the sinuous mode of instability triggered by the spanwise wake-like profile, as confirmed by comparing the numerical results with the absolute/convective instability properties of the family of two-dimensional wakes introduced by Monkewitz (1988). The convective nature of the secondary streak instability implies that the type of bypass transition studied here involves streaks that behave as amplifiers of external noise.

  7. High-speed TV cameras for streak tube readout

    SciTech Connect

    Yates, G.J.; Gallegos, R.A.; Holmes, V.H. ); Turko, B.T. )

    1991-01-01

    Two fast framing TV cameras have been characterized and compared as readout media for imaging of 40 mm diameter streak tube (P-11) phosphor screens. One camera is based upon a Focus-Projection-Scan (FPS) high-speed electrostatic deflected vidicon with 30-mm-diameter PbO target. The other uses an interline transfer charge-coupled device (CCD) with 8.8 {times} 11.4 mm rectangular Si target. The field-of-view (FOV), resolution, responsivity, and dynamic range provided by both cameras when exposed to short duration ({approx} 10 {mu} full width at half maximum (FWHM)) transient illumination followed by a single field readout period of {lt}3 ms are presented. 11 refs., 8 figs., 3 tabs.

  8. Interference effects in angular streaking with a rotating terahertz field

    NASA Astrophysics Data System (ADS)

    Kazansky, A. K.; Bozhevolnov, A. V.; Sazhina, I. P.; Kabachnik, N. M.

    2016-01-01

    A method of angular streaking with a rotating terahertz electric field for photoelectrons produced by femtosecond extreme ultraviolet pulses is suggested and theoretically analyzed. The method can be used for free electron laser (FEL) pulse characterization on a shot-to-shot basis. It is shown that in related measurements an interesting phenomenon appears: formation of very bright and sharp features in the angular resolved electron spectra measured in the plane perpendicular to the collinear beam direction. These features are similar to the conventional caustics in the wave propagation. The caustics are accompanied by a well-developed interference structure. The intensity distribution along the caustic is determined by the envelope of the FEL pulse.

  9. Steering continuum electron dynamics by low-energy attosecond streaking

    NASA Astrophysics Data System (ADS)

    Geng, Ji-Wei; Xiong, Wei-Hao; Xiao, Xiang-Ru; Gong, Qihuang; Peng, Liang-You

    2016-08-01

    A semiclassical model is developed to understand the electronic dynamics in the low-energy attosecond streaking. Under a relatively strong infrared (IR) pulse, the low-energy part of photoelectrons initialized by a single attosecond pulse (SAP) can either rescatter with the ionic core and induce interferences structures in the momentum spectra of the ionized electrons or be recaptured into the Rydberg states. The Coulomb potential plays essential roles in both the electron rescattering and recapturing processes. We find that by changing the time delay between the SAP and the IR pulse, the photoelectrons yield or the population of the Rydberg states can be effectively controlled. The present study demonstrates a fascinating way to steer the electron motion in the continuum.

  10. StreakDet data processing and analysis pipeline for space debris optical observations

    NASA Astrophysics Data System (ADS)

    Virtanen, Jenni; Flohrer, Tim; Muinonen, Karri; Granvik, Mikael; Torppa, Johanna; Poikonen, Jonne; Lehti, Jussi; Santti, Tero; Komulainen, Tuomo; Naranen, Jyri

    We describe a novel data processing and analysis pipeline for optical observations of space debris. The monitoring of space object populations requires reliable acquisition of observational data, to support the development and validation of space debris environment models, the build-up and maintenance of a catalogue of orbital elements. In addition, data is needed for the assessment of conjunction events and for the support of contingency situations or launches. The currently available, mature image processing algorithms for detection and astrometric reduction of optical data cover objects that cross the sensor field-of-view comparably slowly, and within a rather narrow, predefined range of angular velocities. By applying specific tracking techniques, the objects appear point-like or as short trails in the exposures. However, the general survey scenario is always a “track before detect” problem, resulting in streaks, i.e., object trails of arbitrary lengths, in the images. The scope of the ESA-funded StreakDet (Streak detection and astrometric reduction) project is to investigate solutions for detecting and reducing streaks from optical images, particularly in the low signal-to-noise ratio (SNR) domain, where algorithms are not readily available yet. For long streaks, the challenge is to extract precise position information and related registered epochs with sufficient precision. Although some considerations for low-SNR processing of streak-like features are available in the current image processing and computer vision literature, there is a need to discuss and compare these approaches for space debris analysis, in order to develop and evaluate prototype implementations. In the StreakDet project, we develop algorithms applicable to single images (as compared to consecutive frames of the same field) obtained with any observing scenario, including space-based surveys and both low- and high-altitude populations. The proposed processing pipeline starts from the

  11. Illusory Streaks from Corners and Their Perceptual Integration

    PubMed Central

    Roncato, Sergio; Guidi, Stefano; Parlangeli, Oronzo; Battaglini, Luca

    2016-01-01

    Perceptual grouping appears both as organized forms of real figural units and as illusory or “phantom” figures. The phenomenon is visible in the Hermann grid and in configurations which generate color spreading, e.g., “neon effects.” These configurations, generally regular repetitive patterns, appear to be crossed by illusory bands filled with a brighter shade or a colored tinge connecting the various loci of illusory effects. In this work, we explore a particular new illusion showing a grouping effect. It manifests as illusory streaks irradiating from the vertexes of angular contours and connecting pairs of figures nearby. It is only clearly visible when more than one figure is shown, and takes the shape of a net crossing their corners. Although the grouping effect is vivid, the local source of the illusion is completely hidden. Theories explaining this effect as due to the irradiation of illusory streaks (mainly that of Grossberg and Mingolla, 1985a,b) do not fully explain the figural patterns presented here. Illusory effects have already been documented at the angles of various figures, causing them to alter in amplitude and brightness; however, the figure illustrated here appears to have different features and location. Phenomenological observations and an experiment were conducted to assess the role played by geometric and photometric parameters in this illusion. Results showed that sharp angles, in low contrast with the surround, are the main source of the illusion which, however, only becomes visible when at least two figures are close together. These findings are discussed with respect to theories of contour processing and perceptual grouping, and in relation to other illusions. PMID:27445922

  12. Illusory Streaks from Corners and Their Perceptual Integration.

    PubMed

    Roncato, Sergio; Guidi, Stefano; Parlangeli, Oronzo; Battaglini, Luca

    2016-01-01

    Perceptual grouping appears both as organized forms of real figural units and as illusory or "phantom" figures. The phenomenon is visible in the Hermann grid and in configurations which generate color spreading, e.g., "neon effects." These configurations, generally regular repetitive patterns, appear to be crossed by illusory bands filled with a brighter shade or a colored tinge connecting the various loci of illusory effects. In this work, we explore a particular new illusion showing a grouping effect. It manifests as illusory streaks irradiating from the vertexes of angular contours and connecting pairs of figures nearby. It is only clearly visible when more than one figure is shown, and takes the shape of a net crossing their corners. Although the grouping effect is vivid, the local source of the illusion is completely hidden. Theories explaining this effect as due to the irradiation of illusory streaks (mainly that of Grossberg and Mingolla, 1985a,b) do not fully explain the figural patterns presented here. Illusory effects have already been documented at the angles of various figures, causing them to alter in amplitude and brightness; however, the figure illustrated here appears to have different features and location. Phenomenological observations and an experiment were conducted to assess the role played by geometric and photometric parameters in this illusion. Results showed that sharp angles, in low contrast with the surround, are the main source of the illusion which, however, only becomes visible when at least two figures are close together. These findings are discussed with respect to theories of contour processing and perceptual grouping, and in relation to other illusions. PMID:27445922

  13. Dust Devils Seen Streaking Across Mars: PART 1--What Are These?

    NASA Technical Reports Server (NTRS)

    2000-01-01

    PIA02376 [figure removed for brevity, see original site]

    Dark streaks, everywhere! Many Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) images of the middle latitudes of the northern and southern hemispheres of Mars show wild patterns of criss-crossing dark streaks. Many of these streaks are straight and narrow, others exhibit curly arcs, twists, and loops. They often cross over hills, run straight across dunes and ripples, and go through fields of house-sized boulders. The two examples shown above were acquired in the last three months. Both pictures are illuminated by sunlight from the upper left. The first picture (left), showing dark streaks on the rippled flats of Argyre Planitia, covers an area 3 km by 5 km (1.9 by 3.1 miles) at a latitude of 51oS. The second picture (right) shows an area approximately 3 km by 5 km in Promethei Terra at a latitude of 58oS.

    For many months the MOC science team was seeing streaks such as these, but were uncertain how they formed. One speculation was that they might result from the passage of dust devils. Each dust devil would leave a dark streak by removing bright dust from the terrain in its path, revealing a darker surface underneath. An image described by the MOC team in July 1998 showed examples of streaks that were, at the time, speculated to be caused by dust devils.

  14. Flat-field response and geometric distortion measurements of optical streak cameras

    SciTech Connect

    Montgomery, D.S.; Drake, R.P.; Jones, B.A.; Wiedwald, J.D.

    1987-08-01

    To accurately measure pulse amplitude, shape, and relative time histories of optical signals with an optical streak camera, it is necessary to correct each recorded image for spatially-dependent gain nonuniformity and geometric distortion. Gain nonuniformities arise from sensitivity variations in the streak-tube photocathode, phosphor screen, image-intensifier tube, and image recording system. These nonuniformities may be severe, and have been observed to be on the order of 100% for some LLNL optical streak cameras. Geometric distortion due to optical couplings, electron-optics, and sweep nonlinearity not only affects pulse position and timing measurements, but affects pulse amplitude and shape measurements as well. By using a 1.053-..mu..m, long-pulse, high-power laser to generate a spatially and temporally uniform source as input to the streak camera, the combined effects of flat-field response and geometric distortion can be measured under the normal dynamic operation of cameras with S-1 photocathodes. Additionally, by using the same laser system to generate a train of short pulses that can be spatially modulated at the input of the streak camera, we can effectively create a two-dimensional grid of equally-spaced pulses. This allows a dynamic measurement of the geometric distortion of the streak camera. We will discuss the techniques involved in performing these calibrations, will present some of the measured results for LLNL optical streak cameras, and will discuss software methods to correct for these effects. 6 refs., 6 figs.

  15. Stabilization of the hypersonic boundary layer by finite-amplitude streaks

    NASA Astrophysics Data System (ADS)

    Ren, Jie; Fu, Song; Hanifi, Ardeshir

    2016-02-01

    Stabilization of two-dimensional disturbances in hypersonic boundary layer flows by finite-amplitude streaks is investigated using nonlinear parabolized stability equations. The boundary-layer flows at Mach numbers 4.5 and 6.0 are studied in which both first and second modes are supported. The streaks considered here are driven either by the so-called optimal perturbations (Klebanoff-type) or the centrifugal instability (Görtler-type). When the streak amplitude is in an appropriate range, i.e., large enough to modulate the laminar boundary layer but low enough to not trigger secondary instability, both first and second modes can effectively be suppressed.

  16. Improvements in Off-Center Focusing in an X-ray Streak Camera

    SciTech Connect

    McDonald, J W; Weber, F; Holder, J P; Bell, P M

    2003-07-17

    Due to the planar construction of present x-ray streak tubes significant off-center defocusing is observed in both static and dynamic images taken with one-dimensional resolution slits. Based on the streak tube geometry curved photocathodes with radii of curvature ranging from 3.5 to 18 inches have been fabricated. We report initial off-center focusing performance data on the evaluation of these ''improved'' photocathodes in an X-ray streak camera and an update on the theoretical simulations to predict the optimum cathode curvature.

  17. Ultra Fast X-ray Streak Camera for TIM Based Platforms

    SciTech Connect

    Marley, E; Shepherd, R; Fulkerson, E S; James, L; Emig, J; Norman, D

    2012-05-02

    Ultra fast x-ray streak cameras are a staple for time resolved x-ray measurements. There is a need for a ten inch manipulator (TIM) based streak camera that can be fielded in a newer large scale laser facility. The LLNL ultra fast streak camera's drive electronics have been upgraded and redesigned to fit inside a TIM tube. The camera also has a new user interface that allows for remote control and data acquisition. The system has been outfitted with a new sensor package that gives the user more operational awareness and control.

  18. Emissivity spectrum of a large "dark streak" from themis infrared imagery

    SciTech Connect

    Brumby, Steven P.; Vaniman, D. T.; Bish, D. L.

    2003-01-01

    'Dark streaks', also known as 'slope streaks', are unusual surface features found on Mars that are known to appear and fade away on timescales of years. Various explanations have been proposed for their origin and composition, including dry avalanches and wet debris or precipitates from brines. Previous investigations have been based on analysis of panchromatic imagery and altimetry from Viking and Mars Global Surveyor missions. We have obtained an infrared emissivity spectrum of a large dark streak on the north western edge of Olympus Mons, using imagery from the THEMIS instrument on the Mars Odyssey 2001 spacecraft.

  19. Complete Genomic Sequence of Maize Rough Dwarf Virus, a Fijivirus Transmitted by the Small Brown Planthopper.

    PubMed

    Lv, Mingfang; Xie, Li; Yang, Jian; Chen, Jianping; Zhang, Heng-Mu

    2016-01-01

    The nucleotide sequences of the 10 genomic segments of an Italian isolate of maize rough dwarf virus (MRDV) were determined. This first complete genomic sequence of MRDV will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. PMID:26847903

  20. Indiana Corn Dry Mill

    SciTech Connect

    2006-09-01

    The goal of this project is to perform engineering, project design, and permitting for the creation and commercial demonstration of a corn dry mill biorefinery that will produce fuel-grade ethanol, distillers dry grain for animal feed, and carbon dioxide for industrial use.

  1. Foliar diseases of corn

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Leaf blights and spots caused by fungi are some of the most destructive diseases of corn in the US and around the world. Correct identification of the disease is very important in determining the best means of control. For example, gray leaf spot of maize can be caused by one of at least two species...

  2. Streak camera recording of shock wave transit times at large distances using laser illumination

    NASA Astrophysics Data System (ADS)

    Tyburczy, J. A.; Blayney, J. L.; Miller, W. F.; Ahrens, T. J.

    1984-09-01

    A pulsed laser illumination system for streak camera recording of impact-induced shock wave transit times (˜1 μs) during impact experiments is described. Laser illumination of centimeter-sized subjects offers many advantages over diffuse illumination techniques for streak photography. Source-to-sample and sample-to-camera distances of ˜100 to 102 m can be employed. Light filtering, and simultaneous recording of both the impact event and the camera streak rate calibration, can be carried out easily. For use in such a system we describe a Pockels cell controller in which the reference 10-MHz oscillator signal is synchronously divided down to 38 Hz to provide a trigger signal for laser and streak camera testing.

  3. Effect of Process Variables on the Formation of Streak Defects on Anodized Aluminum Extrusions: An Overview

    NASA Astrophysics Data System (ADS)

    Zhu, Hanliang; Couper, Malcolm J.; Dahle, Arne K.

    2012-04-01

    Streak defects are often present on anodized extrusions of 6xxx series aluminum alloys, increasing the fabrication cost of these products. Moreover, streaking often only becomes visible after etching and anodizing treatments, rather than in the as-extruded condition, making it difficult to identify the original causes and influencing factors of these defects. In this paper, various process variables that influence the formation of streak defects on anodized aluminium extrusions are reviewed on the basis of a literature review, industrial practice and experimental results. The influencing factors involved in various processing steps such as billet quality, extrusion process, die design and etching process are considered. Effective measures for preventing the formation of streak defects in industrial extrusion products are discussed.

  4. Standard design for National Ignition Facility x-ray streak and framing cameras

    SciTech Connect

    Kimbrough, J. R.; Bell, P. M.; Bradley, D. K.; Holder, J. P.; Kalantar, D. K.; MacPhee, A. G.; Telford, S.

    2010-10-15

    The x-ray streak camera and x-ray framing camera for the National Ignition Facility were redesigned to improve electromagnetic pulse hardening, protect high voltage circuits from pressure transients, and maximize the use of common parts and operational software. Both instruments use the same PC104 based controller, interface, power supply, charge coupled device camera, protective hermetically sealed housing, and mechanical interfaces. Communication is over fiber optics with identical facility hardware for both instruments. Each has three triggers that can be either fiber optic or coax. High voltage protection consists of a vacuum sensor to enable the high voltage and pulsed microchannel plate phosphor voltage. In the streak camera, the high voltage is removed after the sweep. Both rely on the hardened aluminum box and a custom power supply to reduce electromagnetic pulse/electromagnetic interference (EMP/EMI) getting into the electronics. In addition, the streak camera has an EMP/EMI shield enclosing the front of the streak tube.

  5. Standard design for National Ignition Facility x-ray streak and framing camerasa)

    NASA Astrophysics Data System (ADS)

    Kimbrough, J. R.; Bell, P. M.; Bradley, D. K.; Holder, J. P.; Kalantar, D. K.; MacPhee, A. G.; Telford, S.

    2010-10-01

    The x-ray streak camera and x-ray framing camera for the National Ignition Facility were redesigned to improve electromagnetic pulse hardening, protect high voltage circuits from pressure transients, and maximize the use of common parts and operational software. Both instruments use the same PC104 based controller, interface, power supply, charge coupled device camera, protective hermetically sealed housing, and mechanical interfaces. Communication is over fiber optics with identical facility hardware for both instruments. Each has three triggers that can be either fiber optic or coax. High voltage protection consists of a vacuum sensor to enable the high voltage and pulsed microchannel plate phosphor voltage. In the streak camera, the high voltage is removed after the sweep. Both rely on the hardened aluminum box and a custom power supply to reduce electromagnetic pulse/electromagnetic interference (EMP/EMI) getting into the electronics. In addition, the streak camera has an EMP/EMI shield enclosing the front of the streak tube.

  6. Split ring resonator based THz-driven electron streak camera featuring femtosecond resolution

    PubMed Central

    Fabiańska, Justyna; Kassier, Günther; Feurer, Thomas

    2014-01-01

    Through combined three-dimensional electromagnetic and particle tracking simulations we demonstrate a THz driven electron streak camera featuring a temporal resolution on the order of a femtosecond. The ultrafast streaking field is generated in a resonant THz sub-wavelength antenna which is illuminated by an intense single-cycle THz pulse. Since electron bunches and THz pulses are generated with parts of the same laser system, synchronization between the two is inherently guaranteed. PMID:25010060

  7. Streak detection and analysis pipeline for space-debris optical images

    NASA Astrophysics Data System (ADS)

    Virtanen, Jenni; Poikonen, Jonne; Säntti, Tero; Komulainen, Tuomo; Torppa, Johanna; Granvik, Mikael; Muinonen, Karri; Pentikäinen, Hanna; Martikainen, Julia; Näränen, Jyri; Lehti, Jussi; Flohrer, Tim

    2016-04-01

    We describe a novel data-processing and analysis pipeline for optical observations of moving objects, either of natural (asteroids, meteors) or artificial origin (satellites, space debris). The monitoring of the space object populations requires reliable acquisition of observational data, to support the development and validation of population models and to build and maintain catalogues of orbital elements. The orbital catalogues are, in turn, needed for the assessment of close approaches (for asteroids, with the Earth; for satellites, with each other) and for the support of contingency situations or launches. For both types of populations, there is also increasing interest to detect fainter objects corresponding to the small end of the size distribution. The ESA-funded StreakDet (streak detection and astrometric reduction) activity has aimed at formulating and discussing suitable approaches for the detection and astrometric reduction of object trails, or streaks, in optical observations. Our two main focuses are objects in lower altitudes and space-based observations (i.e., high angular velocities), resulting in long (potentially curved) and faint streaks in the optical images. In particular, we concentrate on single-image (as compared to consecutive frames of the same field) and low-SNR detection of objects. Particular attention has been paid to the process of extraction of all necessary information from one image (segmentation), and subsequently, to efficient reduction of the extracted data (classification). We have developed an automated streak detection and processing pipeline and demonstrated its performance with an extensive database of semisynthetic images simulating streak observations both from ground-based and space-based observing platforms. The average processing time per image is about 13 s for a typical 2k-by-2k image. For long streaks (length >100 pixels), primary targets of the pipeline, the detection sensitivity (true positives) is about 90% for

  8. Characterization results from several commercial soft x-ray streak cameras

    SciTech Connect

    Stradling, G.L.; Studebaker, J.K.; Cavailler, C.; Launspach, J.; Planes, J.

    1986-01-01

    The spatio-temporal performance of four soft x-ray streak cameras has been characterized. The objective in evaluating the performance capability of these instruments is to enable us to optimize experiment designs, to encourage quantitative analysis of streak data and to educate the ultra high speed photography and photonics community about the x-ray detector performance which is available. These measurements have been made collaboratively over the space of two years at the Forge pulsed x-ray source at Los Alamos and at the Ketjak laser facility an CEA Limeil-Valenton. The x-ray pulse lengths used for these measurements at these facilities were 150 psec and 50 psec respectively. The results are presented as dynamically-measured modulation transfer functions. Limiting temporal resolution values were also calculated. Emphasis is placed upon shot noise statistical limitations in the analysis of the data. Space charge repulsion in the streak tube limits the peak flux at ultra short experiments duration times. This limit results in a reduction of total signal and a decrease in signal to noise ratio in the streak image. The four cameras perform well with 20 1p/mm resolution discernable in data from the French C650X, the Hadland X-Chron 540 and the Hamamatsu C1936X streak cameras. The Kentech x-ray streak camera has lower modulation and does not resolve below 10 1p/mm but has a longer photocathode. The C650X bi-lamellar design shows uniform high fidelity recording across both spatial and temporal dimensions. The other three streak cameras show resolution degradation off axis. This must be weighed against a 10X lower streak tube throughput for the C650X.

  9. Measurement of width and intensity of particle streaks in turbulent flows

    NASA Astrophysics Data System (ADS)

    Håkansson, Karl M. O.; Kvick, Mathias; Lundell, Fredrik; Prahl Wittberg, Lisa; Söderberg, L. Daniel

    2013-06-01

    Fibre streaks are observed in experiments with fibre suspensions in a turbulent half-channel flow. The preferential concentration methods, most commonly used to quantify preferential particle concentration, are in one dimension found to be concentration dependent. Two different new streak quantification methods are evaluated, one based on Voronoi analysis and the other based on artificial particles with an assigned fixed width. The width of the particle streaks and a measure of the intensity of the streaks, i.e. streakiness, are sought. Both methods are based on the auto-correlation of a signal, generated by summing images in the direction of the streaks. Common for both methods is a severe concentration dependency, verified in experiments keeping the flow conditions constant while the (very dilute) concentration of fibres is altered. The fixed width method is shown to be the most suitable method, being more robust and less computationally expensive. By assuming the concentration dependence to be related to random noise, an expression is derived, which is shown to make the streak width and the streakiness independent of the concentration even at as low concentrations as 0.05 particles per pixel column in an image. The streakiness is obtained by applying an artificial particle width equal to 20 % of the streak width. This artificial particle width is in this study found to be large enough to smoothen the correlation without altering the streakiness nor the streak width. It is concluded that in order to make quantitative comparisons between different experiments or simulations, the evaluation has to be performed with care and be very well documented.

  10. Intial synchroscan streak camera imaging at the A0 photoinjector

    SciTech Connect

    Lumpkin, A.H.; Ruan, J.; /Fermilab

    2008-04-01

    At the Fermilab A0 photoinjector facility, bunch-length measurements of the laser micropulse and the e-beam micropulse have been done in the past with a single-sweep module of the Hamamatsu C5680 streak camera with an intrinsic shot-to-shot trigger jitter of 10 to 20 ps. We have upgraded the camera system with the synchroscan module tuned to 81.25 MHz to provide synchronous summing capability with less than 1.5-ps FWHM trigger jitter and a phase-locked delay box to provide phase stability of {approx}1 ps over 10s of minutes. This allowed us to measure both the UV laser pulse train at 244 nm and the e-beam via optical transition radiation (OTR). Due to the low electron beam energies and OTR signals, we typically summed over 50 micropulses with 1 nC per micropulse. We also did electron beam bunch length vs. micropulse charge measurements to identify a significant e-beam micropulse elongation from 10 to 30 ps (FWHM) for charges from 1 to 4.6 nC. This effect is attributed to space-charge effects in the PC gun as reproduced by ASTRA calculations. Chromatic temporal dispersion effects in the optics were also characterized and will be reported.

  11. An Optical Streaking Method for Measuring Femtosecond Electron Bunches

    SciTech Connect

    Ding, Yuantao; Bane, Karl L.F.; Huang, Zhirong; /SLAC

    2011-12-14

    The measurement of the ultra-short electron bunch length on the femtosecond time scale constitutes a very challenging problem. In the x-ray free electron laser facilities such as the Linac Coherent Light Source, generation of a sub-ten femtoseconds electron beam with 20pC charge is possible, but direct measurements are very difficult due to the resolution limit of the present diagnostics. We propose a new method here based on the measurement of the electron beam energy modulation induced from laser-electron interaction in a short wiggler. A typical optical streaking method requires a laser wavelength much longer than the electron bunch length. In this paper a laser with its wavelength shorter than the electron bunch length has been adopted, while the slope on the laser intensity envelope is used to distinguish the different periods. With this technique it is possible to reconstruct the bunch longitudinal profile from a single shot measurement. Generation of ultrashort x-ray pulses at femtoseconds (fs) scale is of great interest within synchrotron radiation and free electron laser (FEL) user community. One of the simple methods is to operate the FEL facility at low charge. At the Linac Coherent Light Source (LCLS), we have demonstrated the capability of generating ultrashort electron-beam (e-beam) with a duration of less than 10 fs fwhm using 20 pC charge. The x-ray pulses have been delivered to the x-ray users with a similar or even shorter pulse duration. However, The measurement of such short electron or x-ray pulse length at the fs time-scale constitutes a challenging problem. A standard method using an S-band radio-frequency (rf) transverse deflector has been established at LCLS, which works like a streak camera for electrons and is capable of resolving bunch lengths as short as 25 fs fwhm. With this device, the electrons are transversely deflected by the high-frequency time-variation of the deflecting fields. Increasing the deflecting voltage and rf frequency

  12. Black leaf streak disease affects starch metabolism in banana fruit.

    PubMed

    Saraiva, Lorenzo de Amorim; Castelan, Florence Polegato; Shitakubo, Renata; Hassimotto, Neuza Mariko Aymoto; Purgatto, Eduardo; Chillet, Marc; Cordenunsi, Beatriz Rosana

    2013-06-12

    Black leaf streak disease (BLSD), also known as black sigatoka, represents the main foliar disease in Brazilian banana plantations. In addition to photosynthetic leaf area losses and yield losses, this disease causes an alteration in the pre- and postharvest behavior of the fruit. The aim of this work was to investigate the starch metabolism of fruits during fruit ripening from plants infected with BLSD by evaluating carbohydrate content (i.e., starch, soluble sugars, oligosaccharides, amylose), phenolic compound content, phytohormones, enzymatic activities (i.e., starch phosphorylases, α- and β-amylase), and starch granules. The results indicated that the starch metabolism in banana fruit ripening is affected by BLSD infection. Fruit from infested plots contained unusual amounts of soluble sugars in the green stage and smaller starch granules and showed a different pattern of superficial degradation. Enzymatic activities linked to starch degradation were also altered by the disease. Moreover, the levels of indole-acetic acid and phenolic compounds indicated an advanced fruit physiological age for fruits from infested plots. PMID:23692371

  13. Polar Dunes In Summer Exhibit Frost Patches, Wind Streaks

    NASA Technical Reports Server (NTRS)

    1999-01-01

    Mars Global Surveyor passes over the north polar region of the red planet twelve times each day, offering many opportunities to observe how the polar cap frosts and dunes are changing as the days goby. Right now it is summer in the north. This picture, taken the second week of April 1999, shows darks and dunes and remnant patches of bright frost left over from the winter that ended in July 1998. Dark streaks indicate recent movement of sand. The picture covers an area only 1.4 kilometers (0.9 miles)across and is illuminated from the upper right.

    Malin Space Science Systems and the California Institute of Technology built the MOC using spare hardware from the Mars Observer mission. MSSS operates the camera from its facilities in San Diego, CA. The Jet Propulsion Laboratory's Mars Surveyor Operations Project operates the Mars Global Surveyor spacecraft with its industrial partner, Lockheed Martin Astronautics, from facilities in Pasadena, CA and Denver, CO.

  14. Optimizing Data Recording for the NIF Core Diagnostic X-ray Streak Camera

    SciTech Connect

    Kalantar, D H; Bell, P M; Perry, T S; Sewall, N; Diamond, C; Piston, K

    2000-06-13

    The x-ray streak camera is an important instrument for recording a continuous time history of x-ray emitted from laser target experiments. X-ray streak cameras were used to diagnose experiments in inertially confined fusion and high energy density sciences on the Nova laser. These streak cameras are now used for similar experiments conducted at the OMEGA laser facility, and cameras of this type will be used for experiments on the National Ignition Facility (NIF). The Nova x-ray streak cameras (SSCs) used a sealed optical image intensifier coupled to film to record the streaked x-ray data. In order to develop the core x-ray streak camera for NIF (ref Kimbrough) using a CCD based recording system, we evaluate the performance of the SSCs under a variety of detector configurations. We performed laboratory bench characterization tests of the SSCs to measure the spatial resolution and to evaluate the dynamic range and signal to noise for different configurations of the SSC. We present results of these tests here.

  15. Effects of streaking laser intensity on the characterization of isolated attosecond pulses

    NASA Astrophysics Data System (ADS)

    Wang, He; Khan, Sabih; Chini, Michael; Chen, Shouyuan; Chang, Zenghu

    2009-05-01

    Single isolated attosecond extreme ultraviolet (XUV) pulses can be characterized by streaking photoelectrons using a near infrared (NIR) laser field. Classically, the streaking resolution is determined by the Rayleigh criterion, which requires the minimum NIR intensity of 5.5x10^13 W/cm^2 to resolve 90 as XUV pulses. Under such high NIR intensity, the electrons generated from multi-photon processes overlap with the streaked electrons in the spectrogram, which unavoidably introduces errors in the final XUV reconstruction. When the FROG-CRAB (Frequency-Resolved Optical Gating for Complete Reconstruction of Attosecond Bursts) technique is used to reconstruct the XUV pulses from the spectrogram, it was found that the minimum streaking intensity needed to resolve single attosecond pulses is dependent on the maximum count of the spectrogram. With a peak count of 100 in the spectrogram, chirped attosecond pulses with spectral bandwidth supporting 90-as transform limited pulse durations can be retrieved from the spectrogram with streaking intensity two orders of magnitude smaller than that derived from the Rayleigh criterion. Such low streaking field intensity is desirable to suppress the ATI background, which is important for the characterization of even shorter XUV attosecond pulses because it significantly reduces the intensity constraints on the experiments.

  16. New observations of Bolivian wind streaks by JPL Airborne SAR: Preliminary results

    NASA Technical Reports Server (NTRS)

    Blumberg, Dan G.; Greeley, Ronald

    1995-01-01

    In 1993 NASA's Jet Propulsion Laboratory Airborne Synthetic Aperture Radar system (AIRSAR) was deployed to South America to collect multi-parameter radar data over pre-selected targets. Among the sites targeted was a series of wind streaks located in the Altiplano of Bolivia. The objective of this investigation is to study the effect of wavelength, polarization, and incidence angle on the visibility of wind streaks in radar data. Because this is a preliminary evaluation of the recently acquired data we will focus on one scene and, thus, only on the effects of wavelength and polarization. Wind streaks provide information on the near-surface prevailing winds and on the abundance of winderodible material, such as sand. The potential for a free-flyer radar system that could provide global radar images in multiple wavelengths, polarizations, and incidence angles requires definition of system parameters for mission planning. Furthermore, thousands of wind streaks were mapped from Magellan radar images of Venus; their interpretation requires an understanding of the interaction of radar with wind streaks and the surrounding terrain. Our experiment was conducted on wind streaks in the Altiplano of Bolivia to address these issues.

  17. On the origin and evolution of streaks in polymeric shear flows

    NASA Astrophysics Data System (ADS)

    Page, Jacob; Zaki, Tamer

    2013-11-01

    Streaks are a ubiquitous feature in transitional shear flows of both Newtonian and complex fluids. A model problem is formulated where streaks are generated in response to forcing by a decaying streamwise vortex in an Oldroyd-B fluid, and the effects of inertia and elasticity are examined. The dynamics are found to be largely governed by a single parameter: the ratio of the solvent diffusion to the polymer relaxation timescales. When the time scales are disparate, the ``quasi-Newtonian'' and ``elastic'' dynamics can be distinguished. The ``quasi-Newtonian'' evolution of the streaks in the polymeric flow matches the Newtonian equivalent at the same total (solvent) Reynolds number when polymer relaxation is very fast (slow). The ``elastic'' response is significant, when the polymer relaxation time is long, and leads to significant streak amplification even with very weak inertia. When the diffusion and polymer relaxation timescales are commensurate, the streaks are re-energised in a periodic cycle. This behaviour is enhanced in the instantaneously elastic limit where the governing equation reduces to a wave equation with harmonic forcing. The streak re-energisation is demonstrated to be a superposition of trapped inertio-elastic shear waves.

  18. Spatial relationships among the cellular tapetum, visual streak and rod density in dogs

    PubMed Central

    YAMAUE, Yasuhiro; HOSAKA, Yoshinao Z.; UEHARA, Masato

    2014-01-01

    The dog visual system is well suited to dim light conditions due to rod-dominated retina and the reflective tapetum. The topographical distributions of rods and thickness of the tapetum of the dog were quantified in retinal whole mounts stained with thionine, and spatial relationships among the tapetum, rod density and visual streak of high ganglion cell density were elucidated. The relationship between the retina and tapetum was analyzed in parasagittal sections stained with thionine or hematoxylin-eosin. The tapetum was thick in its center, and the thickest part consisted of 9 to 12 tapetal cell layers. Rod density ranged from 200,000 to 540,000/mm2. Maximum rod density was found in the area dorsal to the visual streak, and the density in that area was significantly higher than the rod density in the visual streak and accorded spatially with the thickest part of the tapetum. The horizontal visual streak was found over the horizontal line through the optic disc in the temporal half and extended slightly into the nasal half. The central area of the highest density of ganglion cells was approximately located midway between the nasal and temporal ends of the visual streak. The visual streak was located within the tapetal area, but ventrally to the thick part of the tapetum. PMID:25728250

  19. Seasonal and secular variation of wind streaks on Mars - An analysis of Mariner 9 and Viking data

    NASA Technical Reports Server (NTRS)

    Thomas, P.; Veverka, J.

    1979-01-01

    Viking orbiter observations extending over 1 Martian year have been used in conjunction with Mariner 9 data obtained in 1971-1972 to study the seasonal and secular behavior of several kinds of wind streaks. Most bright streaks, inferred to consist of dust storm fallout in the lees of obstacles, have changed very little in form or orientation over a period of 3 Martian years. Some are extremely stable and have experienced no effective eolian action over the 3 years. A few bright streaks changed rapidly during global dust storms; these streaks are located in areas subject to both global and topographic winds. Viking images have shown for the first time that dark, erosional streaks are stable from the time of their formation after major dust storms until the onset of the next episode of major storm activity. Available evidence shows that the large, dark streaks in Oxia Palus consist of material deflated from dune fields within the associated craters. These streaks lengthened secularly since 1972; changes appear to occur episodically during southern summer. The great majority of all streaks reflect winds during the period from late southern spring to early southern fall, although some changes occur throughout the year. The global pattern of wind streaks and the variability of the streaks thus depend strongly upon the current south-north asymmetry of seasons on Mars.

  20. Effects of Hot Streak Shape on Rotor Heating in a High-Subsonic Single-Stage Turbine

    NASA Technical Reports Server (NTRS)

    Dorney, Daniel J.; Gundy-Burlet, Karen L.; Norvig, Peter (Technical Monitor)

    1999-01-01

    Experimental data have shown that combustor temperature non-uniformities can lead to the excessive heating of first-stage rotor blades in turbines. This heating of the rotor blades can lead to thermal fatigue and degrade turbine performance. The results of recent studies have shown that variations in the circumferential location (clocking) of the hot streak relative to the first-stage vane airfoils can be used to minimize the adverse effects of the hot streak. The effects of the hot streak/airfoil count ratio on the heating patterns of turbine airfoils have also been evaluated. In the present investigation, three-dimensional unsteady Navier-Stokes simulations have been performed for a single-stage high-pressure turbine operating in high subsonic flow. In addition to a simulation of the baseline turbine, simulations have been performed for circular and elliptical hot streaks of varying sizes in an effort to represent different combustor designs. The predicted results for the baseline simulation show good agreement with the available experimental data. The results of the hot streak simulations indicate: that a) elliptical hot streaks mix more rapidly than circular hot streaks, b) for small hot streak surface area the average rotor temperature is not a strong function of hot streak temperature ratio or shape, and c) hot streaks with larger surface area interact with the secondary flows at the rotor hub endwall, generating an additional high temperature region.

  1. Bean pod mottle virus movement in insect feeding resistant soybeans

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize fine streak virus (MFSV) is a plant-infecting nucleorhabdovirus that is transmitted by the black-faced leafhopper (Graminella nigrifrons [Forbes] Hemiptera: Cicadellidae). Plant-infecting rhabdoviruses are transmitted in a persistent propagative manner, meaning that the vector is also a host ...

  2. 21 CFR 184.1262 - Corn silk and corn silk extract.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Corn silk and corn silk extract. 184.1262 Section... Affirmed as GRAS § 184.1262 Corn silk and corn silk extract. (a) Corn silk is the fresh styles and stigmas of Zea mays L. collected when the corn is in milk. The filaments are extracted with dilute ethanol...

  3. Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms.

    PubMed

    Moellenbeck, D J; Peters, M L; Bing, J W; Rouse, J R; Higgins, L S; Sims, L; Nevshemal, T; Marshall, L; Ellis, R T; Bystrak, P G; Lang, B A; Stewart, J L; Kouba, K; Sondag, V; Gustafson, V; Nour, K; Xu, D; Swenson, J; Zhang, J; Czapla, T; Schwab, G; Jayne, S; Stockhoff, B A; Narva, K; Schnepf, H E; Stelman, S J; Poutre, C; Koziel, M; Duck, N

    2001-07-01

    Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crystals during the sporulation phase of the growth cycle, are encoded by a single operon, and have molecular masses of 14 kDa and 44 kDa. Corn rootworm larvae fed on corn roots expressing the proteins showed histopathological symptoms in the midgut epithelium. PMID:11433280

  4. Callosities, corns, and calluses.

    PubMed Central

    Singh, D.; Bentley, G.; Trevino, S. G.

    1996-01-01

    Inappropriate shoes, abnormal foot mechanics, and high levels of activity produce pressure and friction that lead to corns and calluses. Most lesions can be managed conservatively by proper footwear, orthoses, and, if necessary, regular paring. The lesions usually disappear when the causative mechanical forces are removed. Surgery is rarely indicated and should be specifically aimed at correcting the abnormal mechanical stresses. Images Fig 2 Fig 3 Fig 4 Fig 6 Fig 7 PMID:8646101

  5. Using Maize chlorotic dwarf virus to explore future frontiers in plant virology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize chlorotic dwarf virus (MCDV) causes a chlorosis and stunting disease of corn throughout the Midwest United States. It is a waikavirus transmitted by the leafhopper Graminella nigrifrons. Although waikaviruses are economically important viruses in corn and rice, little is known about the viru...

  6. Chiral oily streaks in a smectic-A liquid crystal.

    PubMed

    Nemitz, Ian R; Ferris, Andrew J; Lacaze, Emmanuelle; Rosenblatt, Charles

    2016-08-21

    The liquid crystal octylcyanobiphenyl (8CB) was doped with the chiral agent CB15 and spin-coated onto a substrate treated for planar alignment of the director, resulting in a film of thickness several hundred nm in the smectic-A phase. In both doped and undoped samples, the competing boundary conditions - planar alignment at the substrate and vertical alignment at the free surface - cause the liquid crystal to break into a series of flattened hemicylinders to satisfy the boundary conditions. When viewed under an optical microscope with crossed polarizers, this structure results in a series of dark and light stripes ("oily streaks") of period ∼1 μm. In the absence of chiral dopant the stripes run perpendicular to the substrate's easy axis. However, when doped with chiral CB15 at concentrations up to c = 4 wt%, the stripe orientation rotates by a temperature-dependent angle φ with respect to the c = 0 stripe orientation, where φ increases monotonically with c. φ is largest just below the nematic - smectic-A transition temperature TNA and decreases with decreasing temperature. As the temperature is lowered, φ relaxes to a steady-state orientation close to zero within ∼1 °C of TNA. We suggest that the rotation phenomenon is a manifestation of the surface electroclinic effect: The rotation is due to the weak smectic order parameter and resulting large director tilt susceptibility with respect to the smectic layer normal near TNA, in conjunction with an effective surface electric field due to polar interactions between the liquid crystal and substrate. PMID:27426740

  7. 21 CFR 184.1321 - Corn gluten.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... principal protein component of corn endosperm. It consists mainly of zein and glutelin. Corn gluten is a... soluble proteins. Corn gluten is also produced as a byproduct during the conversion of the starch in...

  8. Mars Eolian Geology at Airphoto Scales: The Large Wind Streaks of Western Arabia Terra

    NASA Technical Reports Server (NTRS)

    Edgett, Kenneth S.

    2001-01-01

    More than 27,000 pictures at aerial photograph scales (1.5-12 m/pixel) have been acquired by the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) since September 1997. The pictures are valuable for testing hypotheses about geologic history and processes of Mars. Of particular interest are eolian features connected to surface albedo patterns. This work is focused on low-albedo wind streaks, some over 100 km long, in western Arabia Terra. Each streak is widest where it originates at an impact crater (typically 25-150 km diameter). The streaks taper downwind. Within the associated craters there is a lower-albedo surface that, in nearly all observed cases, includes barchan dunes indicative of transport in the same direction as the wind streaks. Upwind of the dunes there is usually an outcrop of layered material that might have served as a source for dune sand. MOC images show that the west Arabia streaks consist of a smooth-surfaced, multiple-meters-thick, mantle (smooth at 1.5 m/pixel) that appears to be superposed on local surfaces. No dunes are present, indicating that down-streak transport of sediment via saltation and traction have not occurred. Two models might explain the observed properties: (1) the streaks consist of dark silt- and clay-sized grains deflated from the adjacent crater interiors and deposited from suspension or (2) they are remnants (protected in the lee of impact crater rims) of a formerly much larger, regional covering of low albedo, smooth-surfaced mantle. The latter hypothesis is based on observation of low albedo mantled surfaces occurring south of west Arabia in Terra Meridiani. For reasons yet unknown, a large fraction of the martian equatorial regions are covered by low albedo, mesa-forming material that lies unconformably atop eroded layered and cratered terrain. Both hypotheses are being explored via continued selective targeting of new MOC images as well as analyses of the new data.

  9. Dust Devils Seen Streaking Across Mars: PART 1--What Are These?

    NASA Technical Reports Server (NTRS)

    2000-01-01

    PIA02376 [figure removed for brevity, see original site]

    PIA02377 [figure removed for brevity, see original site]

    Dark streaks, everywhere! Many Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) images of the middle latitudes of the northern and southern hemispheres of Mars show wild patterns of criss-crossing dark streaks. Many of these streaks are straight and narrow, others exhibit curly arcs, twists, and loops. They often cross over hills, run straight across dunes and ripples, and go through fields of house-sized boulders. The two examples shown above were acquired in the last three months. Both pictures are illuminated by sunlight from the upper left. The first picture (left), showing dark streaks on the rippled flats of Argyre Planitia, covers an area 3 km by 5 km (1.9 by 3.1 miles) at a latitude of 51oS. The second picture (right) shows an area approximately 3 km by 5 km in Promethei Terra at a latitude of 58oS.

    For many months the MOC science team was seeing streaks such as these, but were uncertain how they formed. One speculation was that they might result from the passage of dust devils. Each dust devil would leave a dark streak by removing bright dust from the terrain in its path, revealing a darker surface underneath. An image described by the MOC team in July 1998 showed examples of streaks that were, at the time, speculated to be caused by dust devils.

  10. Cassava virus diseases: biology, epidemiology, and management.

    PubMed

    Legg, James P; Lava Kumar, P; Makeshkumar, T; Tripathi, Leena; Ferguson, Morag; Kanju, Edward; Ntawuruhunga, Pheneas; Cuellar, Wilmer

    2015-01-01

    Cassava (Manihot esculenta Crantz.) is the most important vegetatively propagated food staple in Africa and a prominent industrial crop in Latin America and Asia. Its vegetative propagation through stem cuttings has many advantages, but deleteriously it means that pathogens are passed from one generation to the next and can easily accumulate, threatening cassava production. Cassava-growing continents are characterized by specific suites of viruses that affect cassava and pose particular threats. Of major concern, causing large and increasing economic impact in Africa and Asia are the cassava mosaic geminiviruses that cause cassava mosaic disease in Africa and Asia and cassava brown streak viruses causing cassava brown streak disease in Africa. Latin America, the center of origin and domestication of the crop, hosts a diverse set of virus species, of which the most economically important give rise to cassava frog skin disease syndrome. Here, we review current knowledge on the biology, epidemiology, and control of the most economically important groups of viruses in relation to both farming and cultural practices. Components of virus control strategies examined include: diagnostics and surveillance, prevention and control of infection using phytosanitation, and control of disease through the breeding and promotion of varieties that inhibit virus replication and/or movement. We highlight areas that need further research attention and conclude by examining the likely future global outlook for virus disease management in cassava. PMID:25591878

  11. The sedimentology and dynamics of crater-affiliated wind streaks in western Arabia Terra, Mars and Patagonia, Argentina

    USGS Publications Warehouse

    Rodriguez, J.A.P.; Tanaka, K.L.; Yamamoto, A.; Berman, D.C.; Zimbelman, J.R.; Kargel, J.S.; Sasaki, S.; Jinguo, Y.; Miyamoto, H.

    2010-01-01

    Wind streaks comprise recent aeolian deposits that have been extensively documented on Venus, Earth and Mars. Martian wind streaks are among the most abundant surface features on the planet and commonly extend from the downwind margins of impact craters. Previous studies of wind streaks emerging from crater interior deposits suggested that the mode of emplacement was primarily related to the deposition of silt-sized particles as these settled from plumes. We have performed geologic investigations of two wind streaks clusters; one situated in western Arabia Terra, a region in the northern hemisphere of Mars, and another in an analogous terrestrial site located in southern Patagonia, Argentina, where occurrences of wind streaks emanate from playas within maar craters. In both these regions we have identified bedforms in sedimentary deposits on crater floors, along wind-facing interior crater margins, and along wind streaks. These observations indicate that these deposits contain sand-sized particles and that sediment migration has occurred via saltation from crater interior deposits to wind streaks. In Arabia Terra and in Patagonia wind streaks initiate from crater floors that contain lithic and evaporitic sedimentary deposits, suggesting that the composition of wind streak source materials has played an important role in development. Spatial and topographic analyses suggest that regional clustering of wind streaks in the studied regions directly correlates to the areal density of craters with interior deposits, the degree of proximity of these deposits, and the craters' rim-to-floor depths. In addition, some (but not all) wind streaks within the studied clusters have propagated at comparable yearly (Earth years) rates. Extensive saltation is inferred to have been involved in its propagation based on the studied terrestrial wind streak that shows ripples and dunes on its surface and the Martian counterpart changes orientation toward the downslope direction where it

  12. Ets2-dependent trophoblast signalling is required for gastrulation progression after primitive streak initiation.

    PubMed

    Polydorou, Christiana; Georgiades, Pantelis

    2013-01-01

    Although extraembryonic ectoderm trophoblast signals the embryo for primitive streak initiation, a prerequisite for gastrulation, it is unknown whether it also signals for the progression of gastrulation after primitive streak initiation. Here, using Ets2-/- mice, we show that trophoblast signalling is also required in vivo for primitive streak elongation, completion of intraembryonic mesoderm epithelial-mesenchymal transition and the development of anterior primitive streak derivatives such as the node. We show that Ets2-dependent trophoblast signalling is required for the maintenance of high levels of Nodal and Wnt3 expression in the epiblast and for the induction of Snail expression in the primitive streak, between embryonic day 6.3 and 6.7. Within extraembryonic ectoderm trophoblast, Ets2 maintains the expression of the transcription factors Elf5, Cdx2 and Eomes, and that of the signalling molecule Bmp4. We propose a model that provides a genetic explanation as to how Ets2 in trophoblast mediates the progression of gastrulation within the epiblast. PMID:23552073

  13. Molecular ecology and emergence of tropical plant viruses.

    PubMed

    Fargette, D; Konaté, G; Fauquet, C; Muller, E; Peterschmitt, M; Thresh, J M

    2006-01-01

    An appreciation of the risks caused by emergent plant viruses is critical in tropical areas that rely heavily on agriculture for subsistence and rural livelihood. Molecular ecology, within 10 years, has unraveled the factors responsible for the emergence of several of the economically most important tropical plant viruses: Rice yellow mottle virus (RYMV), Cassava mosaic geminiviruses (CMGs), Maize streak virus (MSV), and Banana streak virus (BSV). A large range of mechanisms--most unsuspected until recently--were involved: recombination and synergism between virus species, new vector biotypes, genome integration of the virus, host adaptation, and long-distance dispersal. A complex chain of molecular and ecological events resulted in novel virus-vector-plant-environment interactions that led to virus emergence. It invariably involved a major agricultural change: crop introduction, cultural intensification, germplasm movement, and new genotypes. A current challenge is now to complement the analysis of the causes by an assessment of the risks of emergence. Recent attempts to assess the risks of emergence of virulent virus strains are described. PMID:16784403

  14. Intravitreal ranibizumab for bilateral choroidal neovascularisation in a patient with angioid streaks

    PubMed Central

    Yilmaz, Ihsan; Ozkaya, Abdullah; Alkin, Zeynep; Yazici, Ahmet Taylan

    2014-01-01

    Angioid streaks are described as irregular lines deep into the retina, configured in a radiating fashion which results from breaks in Bruch’s membrane. Optic nerve head drusen are acellular, globular deposits located within the optic nerve head. Angioid streaks and optic nerve head drusen may coexist in patients with pseudoxanthoma elasticum. Both disorders may cause choroidal neovascularisation. In this case report we aimed to present a 48-year-old man with pseudoxanthoma elasticum. The patient had bilateral choroidal neovascularisation secondary to angioid streaks and was treated with intravitreal ranibizumab injections. Visual acuity was increased and maintained at the same level during the follow-up time. There was no complication related to the injection. PMID:25073527

  15. Effects of diabatic heating on the ageostrophic circulation of an upper tropospheric jet streak

    NASA Technical Reports Server (NTRS)

    Keyser, D. A.; Johnson, D. R.

    1984-01-01

    Higher temporal resolution data from NASA's fourth Atmospheric Variability Experiment (April 24-25, 1975) are used to study the role of diabatic heating within the convective region in altering the ageostrophic mass circulation in the entrance region of the jet streak. The modifying effect this diabatic heating in the mesoscale storm exerts on the synoptic-scale environmental flow through dynamical mechanisms which link the mass circulation of these two scales is examined. The effect of diabatic heating on the ageostrophic motion within the thermally direct circulation of the jet streak and the mesoscale convective complexes is investigated. Diabatic modes of ageostrophic motion that intensify the upper tropospheric jet streak downstream are identified.

  16. Critical evaluation of attosecond time delays retrieved from photoelectron streaking measurements

    NASA Astrophysics Data System (ADS)

    Wei, Hui; Morishita, Toru; Lin, C. D.

    2016-05-01

    A photoelectron streaking experiment which was conceived as a means to extract the electron wave packet of single-photon ionization has also been employed to retrieve time delays in the fundamental photoemission processes. The discrepancies between the time delays thus measured and those from many sophisticated theoretical calculations have generated a great deal of controversy in recent years. Here we present a careful examination of the methods that were used to retrieve the time delays and demonstrate the difficulty of achieving an accuracy of the retrieved time delays of a few to tens of attoseconds in typical streaking measurements. The difficulty owes more to the lower sensitivity of the streaking spectra to the phase of the photoionization transition dipole than to the spectral phase of the attosecond light pulse in the experiment. The retrieved time delay contains extra errors when the attochirp of the attosecond pulse is large so that the dipole phase becomes negligible compared to it.

  17. Impact of laser phase and amplitude noises on streak camera temporal resolution

    SciTech Connect

    Wlotzko, V.; Uhring, W.; Summ, P.

    2015-09-15

    Streak cameras are now reaching sub-picosecond temporal resolution. In cumulative acquisition mode, this resolution does not entirely rely on the electronic or the vacuum tube performances but also on the light source characteristics. The light source, usually an actively mode-locked laser, is affected by phase and amplitude noises. In this paper, the theoretical effects of such noises on the synchronization of the streak system are studied in synchroscan and triggered modes. More precisely, the contribution of band-pass filters, delays, and time walk is ascertained. Methods to compute the resulting synchronization jitter are depicted. The results are verified by measurement with a streak camera combined with a Ti:Al{sub 2}O{sub 3} solid state laser oscillator and also a fiber oscillator.

  18. Unsteady hot streak simulation through a 1-1/2 stage turbine engine

    NASA Astrophysics Data System (ADS)

    Takahashi, R. K.; Ni, R. H.

    1991-06-01

    The temperature redistribution process in a 1-1/2 stage turbine (consisting of a first stator, first rotor, and second stator) was analyzed using an unsteady 3D Euler flow solver. The study concentrated on tracking a hot streak from the inlet of the first stator to the exit of the second stator. The redistribution of the hot streak in the second stator passage was very different from that in the rotor passage, with no signs of temperature segregation in the second stator passage, and with rotor-generated vortices which persist through the second stator passage and partake in redistributing the remains of the hot streak. The unsteady code predicts different time-averaged temperatures and secondary flow in the second stator passage than in the steady multistage code, although the steady code may be sufficient for predicting time-averaged pressure loadings on both rotor and second stator airfoils, and time-averaged secondary flow vortices in the rotor passage.

  19. Imaging plasmonic fields near gold nanospheres in attosecond time-resolved streaked photoelectron spectra

    NASA Astrophysics Data System (ADS)

    Li, Jianxiong; Thumm, Uwe

    2016-05-01

    To study time-resolved photoemission from gold nanospheres, we introduce a quantum-mechanical approach, including the plasmonic near-field-enhancement of the streaking field at the surface of the nanosphere. We use Mie theory to calculate the plasmonically enhanced fields near 10 to 200 nm gold nanospheres, driven by incident near infrared (NIR) or visible laser pulses. We model the gold conduction band in terms of a spherical square well potential. Our simulated streaked photoelectron spectra reveal a plasmonic amplitude enhancement and phase shift related to calculations that exclude the induced plasmonic field. The phase shift is due to the plasma resonance. This suggests the use of streaked photoelectron spectroscopy for imaging the dielectric response and plasmonic field near nanoparticles. Supported by the NSD-EPSCoR program, NSF, and the USDoE.

  20. Absolute calibration method for fast-streaked, fiber optic light collection, spectroscopy systems.

    SciTech Connect

    Johnston, Mark D.; Frogget, Brent; Oliver, Bryan Velten; Maron, Yitzhak; Droemer, Darryl W.; Crain, Marlon D.

    2010-04-01

    This report outlines a convenient method to calibrate fast (<1ns resolution) streaked, fiber optic light collection, spectroscopy systems. Such a system is used to collect spectral data on plasmas generated in the A-K gap of electron beam diodes fielded on the RITS-6 accelerator (8-12MV, 140-200kA). On RITS, light is collected through a small diameter (200 micron) optical fiber and recorded on a fast streak camera at the output of 1 meter Czerny-Turner monochromator (F/7 optics). To calibrate such a system, it is necessary to efficiently couple light from a spectral lamp into a 200 micron diameter fiber, split it into its spectral components, with 10 Angstroms or less resolution, and record it on a streak camera with 1ns or less temporal resolution.

  1. Performances of a solid streak camera based on conventional CCD with nanosecond time resolution

    NASA Astrophysics Data System (ADS)

    Wang, Bo; Bai, Yonglin; Zhu, Bingli; Gou, Yongsheng; Xu, Peng; Bai, XiaoHong; Liu, Baiyu; Qin, Junjun

    2015-02-01

    Imaging systems with high temporal resolution are needed to study rapid physical phenomena ranging from shock waves, including extracorporeal shock waves used for surgery, to diagnostics of laser fusion and fuel injection in internal combustion engines. However, conventional streak cameras use a vacuum tube making thus fragile, cumbersome and expensive. Here we report an CMOS streak camera project consists in reproducing completely this streak camera functionality with a single CMOS chip. By changing the mode of charge transfer of CMOS image sensor, fast photoelectric diagnostics of single point with linear CMOS and high-speed line scanning with array CMOS sensor can be achieved respectively. A fast photoelectric diagnostics system has been designed and fabricated to investigate the feasibility of this method. Finally, the dynamic operation of the sensors is exposed. Measurements show a sample time of 500 ps and a time resolution better than 2 ns.

  2. System for photometric calibration of optoelectronic imaging devices especially streak cameras

    DOEpatents

    Boni, Robert; Jaanimagi, Paul

    2003-11-04

    A system for the photometric calibration of streak cameras and similar imaging devices provides a precise knowledge of the camera's flat-field response as well as a mapping of the geometric distortions. The system provides the flat-field response, representing the spatial variations in the sensitivity of the recorded output, with a signal-to-noise ratio (SNR) greater than can be achieved in a single submicrosecond streak record. The measurement of the flat-field response is carried out by illuminating the input slit of the streak camera with a signal that is uniform in space and constant in time. This signal is generated by passing a continuous wave source through an optical homogenizer made up of a light pipe or pipes in which the illumination typically makes several bounces before exiting as a spatially uniform source field. The rectangular cross-section of the homogenizer is matched to the usable photocathode area of the streak tube. The flat-field data set is obtained by using a slow streak ramp that may have a period from one millisecond (ms) to ten seconds (s), but may be nominally one second in duration. The system also provides a mapping of the geometric distortions, by spatially and temporarily modulating the output of the homogenizer and obtaining a data set using the slow streak ramps. All data sets are acquired using a CCD camera and stored on a computer, which is used to calculate all relevant corrections to the signal data sets. The signal and flat-field data sets are both corrected for geometric distortions prior to applying the flat-field correction. Absolute photometric calibration is obtained by measuring the output fluence of the homogenizer with a "standard-traceable" meter and relating that to the CCD pixel values for a self-corrected flat-field data set.

  3. A grazing incidence x-ray streak camera for ultrafast, single-shot measurements

    SciTech Connect

    Feng, Jun; Engelhorn, K.; Cho, B.I.; Lee, H.J.; Greaves, M.; Weber, C.P.; Falcone, R.W.; Padmore, H. A.; Heimann, P.A.

    2010-02-18

    An ultrafast x-ray streak camera has been realized using a grazing incidence reflection photocathode. X-rays are incident on a gold photocathode at a grazing angle of 20 degree and photoemitted electrons are focused by a large aperture magnetic solenoid lens. The streak camera has high quantum efficiency, 600fs temporal resolution, and 6mm imaging length in the spectral direction. Its single shot capability eliminates temporal smearing due to sweep jitter, and allows recording of the ultrafast dynamics of samples that undergo non-reversible changes.

  4. Linear stability of optimal streaks in the log-layer of turbulent channel flows

    NASA Astrophysics Data System (ADS)

    Alizard, Frédéric

    2015-10-01

    The importance of secondary instability of streaks for the generation of vortical structures attached to the wall in the logarithmic region of turbulent channels is studied. The streaks and their linear instability are computed by solving equations associated with the organized motion that include an eddy-viscosity modeling the effect of incoherent fluctuations. Three friction Reynolds numbers, Reτ = 2000, 3000, and 5000, are investigated. For all flow cases, optimal streamwise vortices (i.e., having the highest potential for linear transient energy amplification) are used as initial conditions. Due to the lift-up mechanism, these optimal perturbations lead to the nonlinear growth of streaks. Based on a Floquet theory along the spanwise direction, we observe the onset of streak secondary instability for a wide range of spanwise wavelengths when the streak amplitude exceeds a critical value. Under neutral conditions, it is shown that streak instability modes have their energy mainly concentrated in the overlap layer and propagate with a phase velocity equal to the mean streamwise velocity of the log-layer. These neutral log-layer modes exhibit a sinuous pattern and have characteristic sizes that are proportional to the wall distance in both streamwise and spanwise directions, in agreement with the Townsend's attached eddy hypothesis (A. Townsend, the structure of turbulent shear flow, Cambridge university press, 1976 2nd edition). In particular, for a distance from the wall varying from y+ ≈ 100 (in wall units) to y ≈ 0.3h, where h is half the height of the channel, the neutral log-layer modes are self-similar with a spanwise width of λz ≈ y/0.3 and a streamwise length of λx ≈ 3λz, independently of the Reynolds number. Based on this observation, it is suggested that compact vortical structures attached to the wall can be ascribed to streak secondary instabilities. In addition, spatial distributions of fluctuating vorticity components show that the onset

  5. Management of corn leafhopper (Homoptera: Cicadellidae) and corn stunt disease in sweet corn using reflective mulch.

    PubMed

    Summers, C G; Stapleton, J J

    2002-04-01

    Plastic reflective mulches significantly reduced populations of corn leafhopper, Dalbulus maidis (DeLong & Wolcott), adults and the incidence of corn stunt disease caused by Spiroplasma kunkelii (CSS) in late planted sweet corn (Zea mays L.). The reflective mulches were more effective than were either foliar or soil applied insecticides in managing both the leafhopper and the pathogen it transmits. Yields of marketable ears were 1.5 to 2 times greater in reflective mulch plots than from fallow plots. This was due to larger ears (individual ear weight and length) rather than an increase in the number of ears. The use of reflective mulches provides an alternative strategy to insecticides in the management of both D. maidis and corn stunt disease. Such a strategy may prove useful to growers in Latin America and to limited resource growers and organic growers in the United States who wish to grow corn without the use of insecticides. PMID:12020008

  6. Aflatoxin in corn hybrids infested at different growth stages with southwestern corn borer (Lepidoptera: Crambidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Aflatoxin is a potent toxin produced by the fungus Aspergillus flavus. Contamination of corn, Zea mays L., with aflatoxin greatly reduces the value of corn grain and is a major impediment to profitable corn production in the South. Infestation of developing corn ears with southwestern corn borer, D...

  7. Screening for corn rootworm (Coleoptera: Chrysomelidae) resistance to transgenic Bt corn in North Dakota

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Western (WCR), Diabrotica virgifera virgifera LeConte, and northern corn rootworms (NCR), D. barberi Smith & Lawrence, are major economic pests of corn in much of the U.S. Corn Belt. Western corn rootworm resistance to transgenic corn expressing Bt (Bacillus thuringiensis) endotoxins has been confi...

  8. 9 CFR 319.102 - Corned beef round and other corned beef cuts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... beef cuts. 319.102 Section 319.102 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... Meats, Unsmoked and Smoked § 319.102 Corned beef round and other corned beef cuts. In preparing “Corned Beef Round” and other corned beef cuts, except “Corned Beef Briskets,” the curing solution shall...

  9. 9 CFR 319.102 - Corned beef round and other corned beef cuts.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... beef cuts. 319.102 Section 319.102 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... Meats, Unsmoked and Smoked § 319.102 Corned beef round and other corned beef cuts. In preparing “Corned Beef Round” and other corned beef cuts, except “Corned Beef Briskets,” the curing solution shall...

  10. Characterization and Functionality of Corn Germ Proteins

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was conducted to evaluate the functional properties of protein extracted from wet-milled corn germ and identify potential applications of the recovered protein. Corn germ comprises 12% of the total weight of normal dent corn and about 29% of the corn protein (moisture-free and oil- free ...

  11. Diapause in northern corn rootworm (Coleoptera: Chrysomelidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Diabroticite corn rootworms are prominent pests of maize and have adapted to both cultural and chemical management methods. In response to a widely used corn-soybean crop rotation in the U.S. Corn Belt over several years, northern corn rootworm (NCR) populations adapted by increasing the proportion ...

  12. 21 CFR 184.1865 - Corn syrup.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Corn syrup. 184.1865 Section 184.1865 Food and....1865 Corn syrup. (a) Corn syrup, commonly called “glucose sirup” or “glucose syrup,” is obtained by... dehydrated form (dried glucose sirup). Depending on the degree of hydrolysis, corn syrup may contain,...

  13. Corn Culture: A Story of Intelligent Design

    ERIC Educational Resources Information Center

    Todd, Jude

    2008-01-01

    Scientists are not sure of how corn was created. There were two competing genetic theories about how corn came to be. One theory maintains that corn had been teased out of a wheatlike grass called teosinte (genus Zea), and the other contends that one now-extinct ancestor of corn had crossed with another grass, "Tripsacum," several millennia ago.…

  14. Characterization of Corn Grains for Ethanol Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Objectives of this study were to understand how the composition of corn kernels and starch structure affect enzyme hydrolysis of starch in dry-grind corn and ethanol yield from yeast fermentation. Four selected corn inbred lines were used in this study. Starch in uncooked dry-grind corn samples sh...

  15. 78 FR 24199 - Streak Products, Inc. v. UTi, United States, Inc.; Notice of Filing of Complaint and Assignment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-24

    ... Streak Products, Inc. v. UTi, United States, Inc.; Notice of Filing of Complaint and Assignment Notice is... Products, Inc. (``Streak''), hereinafter ``Complainant,'' against UTi, United States, Inc. (``UTi...). Complainant also alleges that ``UTi engaged in an unfair or unjustly discriminatory practice in violation...

  16. Effects of Pyramided Bt Corn and Blended Refuges on Western Corn Rootworm and Northern Corn Rootworm (Coleoptera: Chrysomelidae).

    PubMed

    Keweshan, Ryan S; Head, Graham P; Gassmann, Aaron J

    2015-04-01

    The western corn rootworm, Diabrotica virgifera virgifera LeConte, and the northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), are major pests of corn (Zea mays L). Several transgenic corn events producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) kill corn rootworm larvae and reduce injury to corn roots. However, planting of Bt corn imposes selection on rootworm populations to evolve Bt resistance. The refuge strategy and pyramiding of multiple Bt toxins can delay resistance to Bt crops. In this study, we assessed the impact of four treatments--1) non-Bt corn, 2) Cry3Bb1 corn, 3) corn pyramided with Cry3Bb1 and Cry34/35Ab1, and 4) pyramided corn with a blended refuge--on survival, time of adult emergence, and size of western and northern corn rootworm. All treatments with Bt corn led to significant reductions in the number of adults that emerged per plot. However, at one location, we identified Cry3Bb1-resistant western corn rootworm. In some cases Bt treatments reduced size of adults and delayed time of adult emergence, with effects most pronounced for pyramided corn. For both species, the number of adults that emerged from pyramided corn with a blended refuge was significantly lower than expected, based solely on emergence from pure stands of pyramided corn and non-Bt corn. The results of this study indicate that pyramided corn with a blended refuge substantially reduces survival of both western and northern corn rootworm, and as such, should be a useful tool within the context of a broader integrated pest management strategy. PMID:26470183

  17. Isotopic streaks suggest a stripy plume under the Marquesas

    NASA Astrophysics Data System (ADS)

    Chauvel, C.; Blais, S.; Maury, R.; Lewin, E.

    2009-12-01

    underlying the southern and the northern islands is the same. We believe that the stripes are mantle plume characteristics. Their presence and persistent occurrence for the 5 Ma of Marquesas volcanism suggest that isotopically distinct mantle sources cohabitate but do not mix at distances less than 100 km. Furthermore, the isotopic range observed at the island scale is also very large, suggesting melting of extremely heterogeneous plume material. To account for all these observations, as well as the geophysical constraints and the age distribution of the islands, we suggest a model like that of Davaille (Nature, 1999) for general plume structures, and Farnetani and Hofmann (EPSL, 2009) for the internal structure of the Hawaiian plume: several small conduits that originated in a single deep mantle plume bring magma to the surface at different locations at the same time. The material melting in the plume is highly heterogeneous at all scales (10 to 100 km) but because systematic trends occur, an organized pattern must persist through time. The inference is that the heterogeneities in the plume were streaked out vertically and parallel to the direction of plume ascent.

  18. Ethanol extraction of phytosterols from corn fiber

    DOEpatents

    Abbas, Charles; Beery, Kyle E.; Binder, Thomas P.; Rammelsberg, Anne M.

    2010-11-16

    The present invention provides a process for extracting sterols from a high solids, thermochemically hydrolyzed corn fiber using ethanol as the extractant. The process includes obtaining a corn fiber slurry having a moisture content from about 20 weight percent to about 50 weight percent solids (high solids content), thermochemically processing the corn fiber slurry having high solids content of 20 to 50% to produce a hydrolyzed corn fiber slurry, dewatering the hydrolyzed corn fiber slurry to achieve a residual corn fiber having a moisture content from about 30 to 80 weight percent solids, washing the residual corn fiber, dewatering the washed, hydrolyzed corn fiber slurry to achieve a residual corn fiber having a moisture content from about 30 to 80 weight percent solids, and extracting the residual corn fiber with ethanol and separating at least one sterol.

  19. Numerical analysis of three-dimensional unsteady hot streak migration and shock interaction in a turbine stage

    SciTech Connect

    Saxer, A.P.; Felici, H.M.

    1996-04-01

    A three-dimensional unsteady flow computation has been performed for a transonic first turbine stage under the influence of streaks of hot gas exiting the combustion chamber. Realistic flow conditions are obtained by using an unequal stator-to-rotor pitch, a single-streak/multistator channel configuration, and periodic boundary conditions. The resulting unsteady shock wave system and the hot streak migration as well as the shock wave/streak interaction are presented and discussed. In addition, the time average of the periodic unsteady solution is analyzed and compared with a steady-state computation. The steady-state solution matches the time-averaged one in terms of the pressure field and the maximum stagnation temperature on the rotor blade surface. However, the rotor blade temperature patterns are different with a stronger radial secondary flow present in the time-averaged solution due to the retention of the circumferential streak variations at the stator/rotor interface.

  20. Isolation and expression analysis of peanut chlorotic streak caulimovirus (PClSV) full-length transcript (FLt) promoter in transgenic plants.

    PubMed

    Maiti, I B; Shepherd, R J

    1998-03-17

    A promoter fragment from peanut chlorotic streak caulimovirus (PClSV) full-length transcript (FLt) was identified and later modified to have duplicated enhancer domain. The FLt promoter with its single or double enhancer domains, fused with the GUS reporter gene to form chimeric gene constructs, showed a high level of expression of these genes in cells and transgenic plants. The FLt promoter with its double enhancer domain gives an average threefold greater expression of genes compared to the FLt promoter with its single enhancer domain in transgenic plants. In young seedlings the expression was in the order root > leaf > stem. The histochemical GUS assay in young seedlings showed more activity in root tips and leaf midribs, veins, and other vascular tissues. The expression from the PClSV FLt promoter was compared with that from the figwort mosaic virus promoter in transgenic plants. These constitutive promoters were comparable in respect to GUS expression level. PMID:9514942

  1. Diagravitropism in corn roots

    NASA Technical Reports Server (NTRS)

    Leopold, A. C.; Wettlaufer, S. H.

    1988-01-01

    The diagravitropic behavior of Merit corn (Zea mays L.) roots grown in darkness provides an opportunity for comparison of two qualitatively different gravitropic systems. As with positive gravitropism, diagravitropism is shown to require the presence of the root cap, have a similar time course for the onset of curvature, and a similar presentation time. In contrast with positive gravitropism, diagravitropism appears to have a more limited requirement for calcium, for it is insensitive to the elution of calcium by EGTA and insensitive to the subsequent addition of a calcium/EGTA complex. These results are interpreted as indicating that whereas the same sensing system is shared by the two types of gravitropism, separate transductive systems are involved, one for diagravitropism, which is relatively independent of calcium, and one for positive gravitropism, which is markedly dependent on calcium.

  2. Rice Responses and Resistance to Planthopper-Borne Viruses at Transcriptomic and Proteomic Levels.

    PubMed

    Cui, Feng; Zhao, Wan; Luo, Lan; Kang, Le

    2016-01-01

    Rice (Oryza sativa) is one of the most important cereal crops in the world, especially in Asian areas. Rice virus diseases are considered as the most serious threat to rice yields. Most rice viruses are transmitted by hemipteran insects such as planthoppers and leafhoppers. In Asia five rice viruses are transmitted mainly by three planthopper species in a persistent manner: Rice stripe virus, Rice black-streaked dwarf virus, Rice ragged stunt virus, Rice grassy stunt virus, and Southern rice black-streaked dwarf virus. In rice antivirus studies, several individual genes have been shown to function in rice resistance to viruses. Since plant responses to viral infection are complex, system-level omic studies are required to fully understand the responses. Recently more and more omic studies have appeared in the literatures on relationships between planthoppers and viruses, employing microarray, RNA-Seq, small RNA deep sequencing, degradome sequencing, and proteomic analysis. In this paper, we review the current knowledge and progress of omic studies in rice plant responses and resistance to four planthopper-borned viruses. We also discuss progress in the omic study of the interactions of planthoppers and rice viruses. Future research directions and translational applications of fundamental knowledge of virus-vector-rice interactions are proposed. PMID:26363817

  3. Optical Comb Generation for Streak Camera Calibration for Inertial Confinement Fusion Experiments

    SciTech Connect

    Ronald Justin, Terence Davies, Frans Janson, Bruce Marshall, Perry Bell, Daniel Kalantar, Joseph Kimbrough, Stephen Vernon, Oliver Sweningsen

    2008-09-18

    The National Ignition Facility (NIF) at Lawrence Livermore National Laboratory (LLNL) is coming on-line to support physics experimentation for the U.S. Department of Energy (DOE) programs in Inertial Confinement Fusion (ICF) and Stockpile Stewardship (SS). Optical streak cameras are an integral part of the experimental diagnostics instrumentation at NIF. To accurately reduce streak camera data a highly accurate temporal calibration is required. This article describes a technique for simultaneously generating a precise +/- 2 ps optical marker pulse (fiducial reference) and trains of precisely timed, short-duration optical pulses (so-called “comb” pulse trains) that are suitable for the timing calibrations. These optical pulse generators are used with the LLNL optical streak cameras. They are small, portable light sources that, in the comb mode, produce a series of temporally short, uniformly spaced optical pulses, using a laser diode source. Comb generators have been produced with pulse-train repetition rates up to 10 GHz at 780 nm, and somewhat lower frequencies at 664 nm. Individual pulses can be as short as 25-ps FWHM. Signal output is via a fiber-optic connector on the front panel of the generator box. The optical signal is transported from comb generator to streak camera through multi-mode, graded-index optical fiber.

  4. Evaluation of banana hybrids for tolerance to black leaf streak (Mycosphaerella fijiensis Morelet) in Puerto Rico

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In Puerto Rico, bananas (including plantains) are important agricultural commodities; their combined production totaled 133,500 tons in 2008. Black leaf streak (BLS) and Sigatoka leaf spot diseases, caused by Mycosphaerella fijiensis and M. musicola, respectively, are responsible for significant los...

  5. Pedicle streaking: A novel and simple aid in pedicle positioning in free tissue transfer

    PubMed Central

    Aggarwal, Aditya; Singh, Hardeep; Mahendru, Sanjay; Brajesh, Vimalendu; Singh, Sukhdeep; Khare, Ashish; Kothari, Umang; Khazanchi, Rakesh Kumar

    2015-01-01

    Introduction: The pedicle positioning in free tissue transfer is critical to its success. Long thin pedicles are especially prone to this complication where even a slight twist in the perforator can result in flap loss. Pedicles passing through the long tunnels are similarly at risk. Streaking the pedicle with methylene blue is a simple and safe method which increases the safety of free tissue transfer. Materials and Methods: Once the flap is islanded on the pedicle and the vascularity of the flap is confirmed, the pedicle is streaked with methylene blue dye at a distance of 6-7 mm. The streaking starts from the origin of the vessels and continued distally on to the under surface of flap to mark the complete course of the pedicle in alignment. The presence of streaking in some parts and not in rest indicates twist in the pedicle. Observation and Results: Four hundred and sixty five free flaps have been done at our centre in the last 5 years. The overall success rate of free flaps is 95.3% (22 free flap failures). There has not been a single case of pedicle twist leading to flap congestion and failure. Conclusion: This simple and novel method is very reliable for pedicle positioning avoiding any twist necessary for successful free tissue transfer. PMID:26933280

  6. Simultaneous velocity interferometry and electronic streak photography of laser-launched plates

    SciTech Connect

    Paisley, D.L.; Stahl, D.B.; Garcia, I.A.

    1991-01-01

    Laser-launched, miniature, pseudo-one-dimensional flyer plates are evaluated by three distinct optical techniques that may be incorporated into an optical diagnostic system to give a complete understanding of the plate performance. These techniques are: velocity interferometry, streak photography, and pulsed laser stereo photography. 2 refs., 5 figs.

  7. Standard design for National Ignition Facility x-ray streak and framing cameras.

    PubMed

    Kimbrough, J R; Bell, P M; Bradley, D K; Holder, J P; Kalantar, D K; MacPhee, A G; Telford, S

    2010-10-01

    The x-ray streak camera and x-ray framing camera for the National Ignition Facility were redesigned to improve electromagnetic pulse hardening, protect high voltage circuits from pressure transients, and maximize the use of common parts and operational software. Both instruments use the same PC104 based controller, interface, power supply, charge coupled device camera, protective hermetically sealed housing, and mechanical interfaces. Communication is over fiber optics with identical facility hardware for both instruments. Each has three triggers that can be either fiber optic or coax. High voltage protection consists of a vacuum sensor to enable the high voltage and pulsed microchannel plate phosphor voltage. In the streak camera, the high voltage is removed after the sweep. Both rely on the hardened aluminum box and a custom power supply to reduce electromagnetic pulse/electromagnetic interference (EMP/EMI) getting into the electronics. In addition, the streak camera has an EMP/EMI shield enclosing the front of the streak tube. PMID:21034058

  8. Laboratory Simulations and Spectral Analyses of Recurring Slope Streaks on Mars

    NASA Astrophysics Data System (ADS)

    Wing, B.; Irvin, B.; Hibbitts, C.; Mushkin, A.

    2014-12-01

    Low albedo streaks on Martian slopes have been cited as possible evidence for present-day intermittent and repeated surface flow of water, or brine (Mushkin et al., 2010). Also termed as Recurring Slope Lineae (1), such streaks can grow, fade, and recur repeatedly on the same slopes. Although distinguishable by being darker than surrounding terrain slope streaks have no diagnostic spectral absorption features (2). A leading hypothesis is formation by multiple wetting and drying events. Laboratory investigations have previously explored this possibility (e.g 3). When wetted with brines, soils darken, but as the sample dries, it brightens again. Wetting also results in absorption bands near 1.5 and 2 microns, which are not detected in spectra of slope streaks. Additionally, dried brines of most salts such as MgSO4, or other sulfates and many chlorides are brighter than Martian soils. However, iron chlorides are a salt that have lower albedo than most other salts and may present a mechanism for darkening slope streaks without inducing a spectral absorption feature. To explore this hypothesis, we have begun to conduct experiments investigating the spectra of iron chloride chloride solutions wetting palagonite and subsequently drying under Martian atmospheric conditions. Preliminary experiments demonstrate that FeIII chloride dried onto palagonite has no absorption features in the NIR and SWIR and remains dark and red. However, these chlorides will oxidize under terrestrial conditions forming Fe2O3 as they dry. We have constructed an environmental chamber that mimics Martian oxygen fugacity though a combination of vacuum and N2 purging, allowing for sample wetting and drying while concurrently taking spectra from 0.4 to 2.4 microns. Results from this experimental setup under Martian atmospheric conditions will be presented. References: (1) McEwen et al., (2011) Science, 333, 740-743, (2) Mushkin et al., (2010) Geophys. Res. Lett., 37, L22201, doi: 10.1029/2010GL044535

  9. NON-UNIFORM PATTERN OF SEQUENCE DIVERGENCE BETWEEN OAT NECROTIC MOTTLE VIRUS AND WHEAT STREAK MOSAIC VIRUS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sequence of the 3'-terminal 1729 nucleotides (nts) of two ONMV isolates were determined and compared with sequences of WSMV isolates Czech Republic, Hungary, Iran, Mexico, Russia, Turkey and the U.S.A. The WSMV sequences shared 73-74% (nt) and 79¿81% (amino acid [aa]) identity with ONMV. In cont...

  10. 21 CFR 155.131 - Canned field corn.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Canned field corn. 155.131 Section 155.131 Food... Canned field corn. (a) Identity. (1) Canned field corn conforms to the definition and standard of... corn by § 155.130(a), except that the corn ingredient consists of succulent field corn or a mixture...

  11. 21 CFR 155.131 - Canned field corn.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Canned field corn. 155.131 Section 155.131 Food... Canned field corn. (a) Identity. (1) Canned field corn conforms to the definition and standard of... corn by § 155.130(a), except that the corn ingredient consists of succulent field corn or a mixture...

  12. 21 CFR 155.131 - Canned field corn.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Canned field corn. 155.131 Section 155.131 Food... Canned field corn. (a) Identity. (1) Canned field corn conforms to the definition and standard of... corn by § 155.130(a), except that the corn ingredient consists of succulent field corn or a mixture...

  13. 21 CFR 155.131 - Canned field corn.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Canned field corn. 155.131 Section 155.131 Food... Canned field corn. (a) Identity. (1) Canned field corn conforms to the definition and standard of... corn by § 155.130(a), except that the corn ingredient consists of succulent field corn or a mixture...

  14. 21 CFR 155.131 - Canned field corn.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Canned field corn. 155.131 Section 155.131 Food... Canned field corn. (a) Identity. (1) Canned field corn conforms to the definition and standard of... corn by § 155.130(a), except that the corn ingredient consists of succulent field corn or a mixture...

  15. Field-based assessment of resistance to Bt Corn by Western Corn Rootworm

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Western corn rootworm, Diabrotica virgifera virgifera LeConte, is a serious pest of corn and is managed with Bt corn that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). Beginning in 2009, severe injury to Bt corn producing Cry3Bb1 was observed in some cornfields ...

  16. Utilisation of Corn (Zea mays) Bran and Corn Fiber in the Production of Food Components

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Over the past decade, the demand for ethanol has increased dramatically. Demand for other products of corn milling, such as starches and sweeteners, is also expected to increase. With the increase in demand for industrial and food use of corn, the production of byproducts, such as corn fiber, corn...

  17. 77 FR 10617 - Wellsboro & Corning Railroad, LLC-Acquisition and Operation Exemption-Wellsboro & Corning...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-22

    ... Surface Transportation Board Wellsboro & Corning Railroad, LLC--Acquisition and Operation Exemption--Wellsboro & Corning Railroad Company Wellsboro & Corning Railroad, LLC (WCLLC), a noncarrier, has filed a verified notice of exemption under 49 CFR 1150.31 to acquire from Wellsboro & Corning Railroad Company...

  18. Resistance to Bt corn by western corn rootworm (Coleoptera: Chrysomelidae) in the U.S. corn belt

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Transgenic Bt corn hybrids that produce insecticidal proteins from the bacterium Bacillus thuringiensis Berliner have become the standard insect management tactic across the United States Corn Belt. Widespread planting of Bt corn creates intense selection pressure for target insects to develop resis...

  19. Corn-in-chip: Mesofluidic Device for Corn Root

    NASA Astrophysics Data System (ADS)

    Kreis, Kevin; Ryu, Sangjin

    2015-03-01

    Plants have a collection of beneficial microorganisms in a region surrounding their roots called the rhizosphere. Although rhizosphere management could increase crop yield, little is known about the interaction between plant roots and their associated microorganisms. Thus we aim to simulate the rhizosphere and monitor root-microbe interactions in the lab environment, and have chosen corn as a model plant because of its economic significance. Here we present our preliminary study to develop a transparent mesofluidic device accommodating the root of corn seedlings into its channel and allowing further growth of the root.

  20. Optical streak camera images of wire-array z-pinches on the 1-MA COBRA pulsed power generator

    NASA Astrophysics Data System (ADS)

    McBride, Ryan; Pikuz, Sergei; Blesener, Isaac; Zhao, Yu Tao; Greenly, John; Hammer, David; Kusse, Bruce

    2006-10-01

    Initial optical streak camera imaging experiments of wire array z-pinches on the 1 MA COBRA pulsed power generator are presented. The imaging system makes use of a Hamamatsu C7700 streak unit, which is coupled to a V7669-06 image intensifier with an MCP, and a C4742-98 CCD camera. A long focal length optical system is employed to relay the z-pinch produced light from the experiment chamber to the input slit of the streak camera -- a total transmission distance of approximately 14 m. The optical streak camera images produced, along with data from other supporting diagnostics, are presented for z-pinch implosions of various wire array geometries and materials.

  1. Initial tests of the dual-sweep streak camera system planned for APS particle-beam diagnostics

    SciTech Connect

    Lumpkin, A.; Yang, B.; Gai, W.; Cieslik, W.

    1995-07-01

    Initial tests of a dual-sweep streak system planned for use on the Advanced Photon Source (APS) have been performed using assets of the Argonne Wakefield Accelerator (AWA) facility. The short light pulses from the photoelectric injector drive laser in both the visible ({lambda}=496 nm, {Delta}t{approximately}1.5 ps (FWHM)), and the ultraviolet ({lambda}=248 nm, {Delta}t{approximately}5 ps (FWHM)) were used. Both a UV-visible S20 photocathode streak tube and a UV-to-x-ray Au photocathode streak tube were tested. Calibration data with an etalon were also obtained. A sample of dual-sweep streak data using optical synchrotron radiation on the APS injector synchrotron is also presented.

  2. Streaked radiography of an irradiated foam sample on the National Ignition Facility

    SciTech Connect

    Cooper, A. B. R.; Schneider, M. B.; MacLaren, S. A.; Young, P. E.; Hsing, W. W.; Seugling, R.; Foord, M. E.; Sain, J. D.; May, M. J.; Marrs, R. E.; Maddox, B. R.; Lu, K.; Dodson, K.; Smalyuk, V.; Moore, A. S.; Graham, P.; Foster, J. M.; Back, C. A.; Hund, J. F.

    2013-03-15

    Streaked x-ray radiography images of annular patterns in an evolving tantalum oxide foam under the influence of a driven, subsonic radiation wave were obtained on the National Ignition Facility. This is the first successful radiography measurement of the evolution of well-defined foam features under a driven, subsonic wave in the diffusive regime. A continuous record of the evolution was recorded on an x-ray streak camera, using a slot-apertured point-projection backlighter with an 8 ns nickel source (7.9 keV). Radiography images were obtained for four different annular patterns, which were corrected using a source-dependent flat-field image. The evolution of the foam features was well-modeled using the 3D KULL radiation hydrodynamics code. This experimental and modeling platform can be modified for scaled high-energy-density laboratory astrophysics experiments.

  3. Effects of diabatic heating on the ageostrophic circulation of an upper tropospheric jet streak

    NASA Technical Reports Server (NTRS)

    Keyser, D. A.; Johnson, D. R.

    1982-01-01

    Interaction between the mass circulation within a mesoscale convective complex (MCC) and a direct mass circulation in the entrance region of an upper tropospheric polar jet streak was examined within the isentropic structure to investigate mechanisms responsible for linking these two scales of motion. The results establish that latent heating in the MCC modifies the direct mass circulation in the jet streak entrance region through the diabatically induced components of ageostrophic motion analyzed within isentropic coordinates. Within the strong mesoscale mass circulation of each MCC, strong horizontal mass flux convergence into the MCC at low levels is balanced by strong horizontal mass flux divergence away from the convergence at upper levels. Locations of large diabatic heating rates correspond well to the MCC position for each case; diabatic heating forces the upward vertical branch for the mesoscale mass circulation.

  4. Time-Resolved Spectra of Dense Plasma Focus Using Spectrometer, Streak Camera, CCD Combination

    SciTech Connect

    F. J. Goldin, B. T. Meehan, E. C. Hagen, P. R. Wilkins

    2010-10-01

    A time-resolving spectrographic instrument has been assembled with the primary components of a spectrometer, image-converting streak camera, and CCD recording camera, for the primary purpose of diagnosing highly dynamic plasmas. A collection lens defines the sampled region and couples light from the plasma into a step index, multimode fiber which leads to the spectrometer. The output spectrum is focused onto the photocathode of the streak camera, the output of which is proximity-coupled to the CCD. The spectrometer configuration is essentially Czerny–Turner, but off-the-shelf Nikon refraction lenses, rather than mirrors, are used for practicality and flexibility. Only recently assembled, the instrument requires significant refinement, but has now taken data on both bridge wire and dense plasma focus experiments.

  5. Time-resolved spectra of dense plasma focus using spectrometer, streak camera, and CCD combination

    SciTech Connect

    Goldin, F. J.; Meehan, B. T.; Hagen, E. C.; Wilkins, P. R.

    2010-10-15

    A time-resolving spectrographic instrument has been assembled with the primary components of a spectrometer, image-converting streak camera, and CCD recording camera, for the primary purpose of diagnosing highly dynamic plasmas. A collection lens defines the sampled region and couples light from the plasma into a step index, multimode fiber which leads to the spectrometer. The output spectrum is focused onto the photocathode of the streak camera, the output of which is proximity-coupled to the CCD. The spectrometer configuration is essentially Czerny-Turner, but off-the-shelf Nikon refraction lenses, rather than mirrors, are used for practicality and flexibility. Only recently assembled, the instrument requires significant refinement, but has now taken data on both bridge wire and dense plasma focus experiments.

  6. Protocol for observing molecular dipole excitations by attosecond self-streaking

    NASA Astrophysics Data System (ADS)

    Wachter, Georg; Nagele, Stefan; Sato, Shunsuke A.; Pazourek, Renate; Wais, Michael; Lemell, Christoph; Tong, Xiao-Min; Yabana, Kazuhiro; Burgdörfer, Joachim

    2015-12-01

    We propose a protocol to probe the ultrafast evolution and dephasing of coherent electronic excitation in molecules in the time domain by the intrinsic streaking field generated by the molecule itself. Coherent electronic motion in the endohedral fullerene Ne@C 60 is initiated by a moderately intense femtosecond UV-visible pulse leading to coherent oscillations of the molecular dipole moment that persist after the end of the laser pulse. The resulting time-dependent molecular near field is probed through the momentum modulation of photoemission from the central neon atom by a time-delayed attosecond XUV pulse. Our ab initio time-dependent density functional theory and classical trajectory simulations predict that this self-streaking signal accurately traces the molecular dipole oscillations in real time. We discuss the underlying processes and give an analytical model that captures the essence of our ab initio simulations.

  7. Extra-embryonic Wnt3 regulates the establishment of the primitive streak in mice

    PubMed Central

    Yoon, Yeonsoo; Huang, Tingting; Tortelote, Giovane G.; Wakamiya, Maki; Hadjantonakis, Anna-Katerina; Behringer, Richard R.; Rivera-Pérez, Jaime A.

    2015-01-01

    The establishment of the head to tail axis at early stages of development is a fundamental aspect of vertebrate embryogenesis. In mice, experimental embryology, genetics and expression studies have suggested that the visceral endoderm, an extra-embryonic tissue, plays an important role in anteroposterior axial development. Here we show that absence of Wnt3 in the posterior visceral endoderm leads to delayed formation of the primitive streak and that interplay between anterior and posterior visceral endoderm restricts the position of the primitive streak. Embryos lacking Wnt3 in the visceral endoderm, however, appear normal by E9.5. Our results suggest a model for axial development in which multiple signals are required for anteroposterior axial development in mammals. PMID:25907228

  8. Recording the synchrotron radiation by a picosecond streak camera for bunch diagnostics in cyclic accelerators

    NASA Astrophysics Data System (ADS)

    Vereshchagin, A. K.; Vorob'ev, N. S.; Gornostaev, P. B.; Dorokhov, V. L.; Kryukov, S. S.; Lozovoi, V. I.; Meshkov, O. I.; Nikiforov, D. A.; Smirnov, A. V.; Shashkov, E. V.; Schelev, M. Ya

    2016-02-01

    A PS-1/S1 picosecond streak camera with a linear sweep is used to measure temporal characteristics of synchrotron radiation pulses on a damping ring (DR) at the Budker Institute of Nuclear Physics (BINP) of the Siberian Branch of the Russian Academy of Sciences (Novosibirsk). The data obtained allow a conclusion as to the formation processes of electron bunches and their 'quality' in the DR after injection from the linear accelerator. The expediency of employing the streak camera as a part of an optical diagnostic accelerator complex for adjusting the injection from a linear accelerator is shown. Discussed is the issue of designing a new-generation dissector with a time resolution up to a few picoseconds, which would allow implementation of a continuous bunch monitoring in the DR during mutual work with the electron-positron colliders at the BINP.

  9. Route to One Atomic Unit of Time: Development of a Broadband Attosecond Streak Camera

    NASA Astrophysics Data System (ADS)

    Zhao, Kun; Zhang, Qi; Chini, Michael; Chang, Zenghu

    A new attosecond streak camera based on a three-meter-long magnetic-bottle time-of-flight electron spectrometer (MBES) is developed. The temporal resolution of the photoelectron detection system is measured to be better than 250 ps, which is sufficient to achieve an energy resolution of 0.5 eV at 150 eV photoelectron energy. In preliminary experiments, a 94-as isolated XUV pulse was generated and characterized. With a new algorithm to retrieve the amplitude and phase of XUV pulses (PROOF—phase retrieval by omega oscillation filtering), the attosecond streak camera will be able to characterize isolated attosecond pulses as short as one atomic unit of time (25 as).

  10. Single-shot visualization of evolving laser wakefields using an all-optical streak camera.

    PubMed

    Li, Zhengyan; Tsai, Hai-En; Zhang, Xi; Pai, Chih-Hao; Chang, Yen-Yu; Zgadzaj, Rafal; Wang, Xiaoming; Khudik, V; Shvets, G; Downer, M C

    2014-08-22

    We visualize ps-time-scale evolution of an electron density bubble--a wake structure created in atmospheric density plasma by an intense ultrashort laser pulse--from the phase "streak" that the bubble imprints onto a probe pulse that crosses its path obliquely. Phase streaks, recovered in one shot using frequency-domain interferometric techniques, reveal the formation, propagation, and coalescence of the bubble within a 3 mm long ionized helium gas target. 3D particle-in-cell simulations validate the observed density-dependent bubble evolution, and correlate it with the generation of a quasimonoenergetic ∼ 100 MeV electron beam. The results provide a basis for understanding optimized electron acceleration at a plasma density n(e) ≈ 2 × 10(19) cm(-3), inefficient acceleration at lower density, and dephasing limits at higher density. PMID:25192102

  11. Temporal resolution limit estimation of x-ray streak cameras using a CsI photocathode

    SciTech Connect

    Li, Xiang; Gu, Li; Zong, Fangke; Zhang, Jingjin; Yang, Qinlao

    2015-08-28

    A Monte Carlo model is developed and implemented to calculate the characteristics of x-ray induced secondary electron (SE) emission from a CsI photocathode used in an x-ray streak camera. Time distributions of emitted SEs are investigated with an incident x-ray energy range from 1 to 30 keV and a CsI thickness range from 100 to 1000 nm. Simulation results indicate that SE time distribution curves have little dependence on the incident x-ray energy and CsI thickness. The calculated time dispersion within the CsI photocathode is about 70 fs, which should be the temporal resolution limit of x-ray streak cameras that use CsI as the photocathode material.

  12. A novel compact high speed x-ray streak camera (invited)

    SciTech Connect

    Hares, J. D.; Dymoke-Bradshaw, A. K. L.

    2008-10-15

    Conventional in-line high speed streak cameras have fundamental issues when their performance is extended below a picosecond. The transit time spread caused by both the spread in the photoelectron (PE) ''birth'' energy and space charge effects causes significant electron pulse broadening along the axis of the streak camera and limits the time resolution. Also it is difficult to generate a sufficiently large sweep speed. This paper describes a new instrument in which the extraction electrostatic field at the photocathode increases with time, converting time to PE energy. A uniform magnetic field is used to measure the PE energy, and thus time, and also focuses in one dimension. Design calculations are presented for the factors limiting the time resolution. With our design, subpicosecond resolution with high dynamic range is expected.

  13. Mach-zehnder based optical marker/comb generator for streak camera calibration

    SciTech Connect

    Miller, Edward Kirk

    2015-03-03

    This disclosure is directed to a method and apparatus for generating marker and comb indicia in an optical environment using a Mach-Zehnder (M-Z) modulator. High speed recording devices are configured to record image or other data defining a high speed event. To calibrate and establish time reference, the markers or combs are indicia which serve as timing pulses (markers) or a constant-frequency train of optical pulses (comb) to be imaged on a streak camera for accurate time based calibration and time reference. The system includes a camera, an optic signal generator which provides an optic signal to an M-Z modulator and biasing and modulation signal generators configured to provide input to the M-Z modulator. An optical reference signal is provided to the M-Z modulator. The M-Z modulator modulates the reference signal to a higher frequency optical signal which is output through a fiber coupled link to the streak camera.

  14. The 1971 corn blight watch experiment

    NASA Technical Reports Server (NTRS)

    Clifton, J. W.

    1972-01-01

    The successful fulfillment of the objectives for the 1971 corn blight watch experiment is reported. The objectives were: (1) detect the development and spread of corn blight during the growing season across the Corn Belt; (2) assess different levels of infection in the Corn Belt; (3) amplify data acquired by ground observations to better appraise current blight status and the probable impact on crop production; and (4) estimate through extrapolation the applicability of these techniques to similar situations occurring in the future.

  15. 21 CFR 184.1865 - Corn syrup.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Corn syrup. 184.1865 Section 184.1865 Food and... Substances Affirmed as GRAS § 184.1865 Corn syrup. (a) Corn syrup, commonly called “glucose sirup” or “glucose syrup,” is obtained by partial hydrolysis of corn starch with safe and suitable acids or...

  16. 21 CFR 184.1865 - Corn syrup.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Corn syrup. 184.1865 Section 184.1865 Food and... Substances Affirmed as GRAS § 184.1865 Corn syrup. (a) Corn syrup, commonly called “glucose sirup” or “glucose syrup,” is obtained by partial hydrolysis of corn starch with safe and suitable acids or...

  17. EXPRESSION OF THE MAIZE MOSAIC VIRUS GLYCOPROTEIN IN INSECT CELLS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize mosaic virus (genus Nucleorhabdovirus, family Rhabdoviridae) is transmitted in a persistent-propagative manner by Peregrinus maidis, the corn planthopper. Like other rhabdoviruses, the MMV genome encodes a surface glycoprotein that is likely involved in virus attachment and entry into host ce...

  18. High-Speed Observer: Automated Streak Detection for the Aerospike Engine

    NASA Technical Reports Server (NTRS)

    Rieckhoff, T. J.; Covan, M. A.; OFarrell, J. M.

    2001-01-01

    A high-frame-rate digital video camera, installed on test stands at Stennis Space Center (SSC), has been used to capture images of the aerospike engine plume during test. These plume images are processed in real time to detect and differentiate anomalous plume events. Results indicate that the High-Speed Observer (HSO) system can detect anomalous plume streaking events that are indicative of aerospike engine malfunction.

  19. Molecular organization of the cholesteryl ester droplets in the fatty streaks of human aorta.

    PubMed Central

    Engelman, D M; Hillman, G M

    1976-01-01

    X-ray diffraction patterns from human arterial specimens containing atherosclerotic fatty streak lesions exhibited a single sharp reflection, corresponding to a structural spacing of about 35 A. Specimens without lesions did not. When specimens with fatty streaks were heated, an order-to-disorder phase transition was revealed by the disappearance of the sharp reflection. The transition was thermally reversible and its temperature varied from aorta to aorta over a range from 28 degrees to 42 degrees C. Since cholesteryl ester droplets are a major component of fatty streaks, comparison studies were made of the diffraction behavior from pure cholesteryl esters. We found that the diffraction patterns of the fatty streak material could be accounted for by the organization of the cholesteryl esters into a liquid-crystalline smectic phase that melts from the smectic to a less ordered phase upon heating. When combined with the conclusions of others from polarized light microscopy, our study shows that a droplet in the smectic phase has well-defined concentric layers of lipid molecules. In each layer, the long axes of the molecules have a net radial orientation with respect to the droplet, but the side-to-side organization is disordered. We suggest that the accessibility of portions of the lipids for specific binding to enzymes or transport proteins may be restricted when they are in the smectic state, and that exchange of lipids with surrounding membranes or other potential binding sites may likewise be inhibited. The restriction in the smectic phase should be greater than in the less ordered phases that exist at higher temperatures. Images PMID:965500

  20. 2-ps Hard X-Ray Streak Camera Measurements at Sector 7 Beamline of the Advanced Photon Source

    SciTech Connect

    Chollet, M.; Ahr, B.; Walko, D.A.; Rose-Petruck, C.; Adams, B.

    2011-08-02

    A hard X-ray streak camera capable of 2-ps time resolution is in operation at the Sector 7 beamline of the Advanced Photon Source. It is used for laser-pump, X-ray probe experiments using the Ti:Sapphire femtosecond laser system installed on the beamline. This streak camera, combined with standardized and prealigned experimental setups, can perform time-resolved liquid-phase absorption spectroscopy, reflectivity, and diffraction experiments.

  1. "King Corn": Teaching the Food Crisis

    ERIC Educational Resources Information Center

    Swinehart, Tim

    2012-01-01

    "King Corn" is in so many ways the story of how government food policy has entirely remade the food landscape in the United States over the last 40 years. From the massive expansion of the number of acres of corn grown across the country, to the ever-increasing ways that corn is incorporated into the food production process, to the industrial…

  2. 9 CFR 319.100 - Corned beef.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... permitting that use in this subchapter or 9 CFR Chapter III, Subchapter E, or in 21 CFR Chapter I, Subchapter... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Corned beef. 319.100 Section 319.100... Corned beef. “Corned Beef” shall be prepared from beef briskets, navels, clods, middle ribs,...

  3. Geographic information systems in corn rootworm management

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn rootworms (Diabrotica spp. Coleoptera: Chrysomelidae) are serious pests of corn (Zea mays) in the United States and Europe. Control measures for corn rootworms (CRW) were historically based upon chemical pesticides and crop rotation. Pesticide use created environmental and economic concerns. In...

  4. Does Bt Corn Really Produce Tougher Residues

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bt corn hybrids produce insecticidal proteins that are derived from a bacterium, Bacillus thuringiensis. There have been concerns that Bt corn hybrids produce residues that are relatively resistant to decomposition. We conducted four experiments that examined the decomposition of corn residues und...

  5. 21 CFR 184.1865 - Corn syrup.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Substances Affirmed as GRAS § 184.1865 Corn syrup. (a) Corn syrup, commonly called “glucose sirup” or “glucose syrup,” is obtained by partial hydrolysis of corn starch with safe and suitable acids or enzymes. It may also occur in the dehydrated form (dried glucose sirup). Depending on the degree of...

  6. 21 CFR 184.1865 - Corn syrup.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Substances Affirmed as GRAS § 184.1865 Corn syrup. (a) Corn syrup, commonly called “glucose sirup” or “glucose syrup,” is obtained by partial hydrolysis of corn starch with safe and suitable acids or enzymes. It may also occur in the dehydrated form (dried glucose sirup). Depending on the degree of...

  7. Alfalfa: A Companion Crop with Corn

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn grain-based ethanol is the major form of biofuel production in the USA. Corn is an attractive biofuel crop because it is easy to manage, process, and ship; is high yielding; and has significant industry support and research. However, there are concerns about growing corn in rotation with soyb...

  8. Development of intelligent control system for X-ray streak camera in diagnostic instrument manipulator

    NASA Astrophysics Data System (ADS)

    Pei, Chengquan; Wu, Shengli; Tian, Jinshou; Liu, Zhen; Fang, Yuman; Gao, Guilong; Liang, Lingliang; Wen, Wenlong

    2015-11-01

    An intelligent control system for an X ray streak camera in a diagnostic instrument manipulator (DIM) is proposed and implemented, which can control time delay, electric focusing, image gain adjustment, switch of sweep voltage, acquiring environment parameters etc. The system consists of 16 A/D converters and 16 D/A converters, a 32-channel general purpose input/output (GPIO) and two sensors. An isolated DC/DC converter with multi-outputs and a single mode fiber were adopted to reduce the interference generated by the common ground among the A/D, D/A and I/O. The software was designed using graphical programming language and can remotely access the corresponding instrument from a website. The entire intelligent control system can acquire the desirable data at a speed of 30 Mb/s and store it for later analysis. The intelligent system was implemented on a streak camera in a DIM and it shows a temporal resolution of 11.25 ps, spatial distortion of less than 10% and dynamic range of 279:1. The intelligent control system has been successfully used in a streak camera to verify the synchronization of multi-channel laser on the Inertial Confinement Fusion Facility.

  9. High-Throughput Single-Cell Cultivation on Microfluidic Streak Plates.

    PubMed

    Jiang, Cheng-Ying; Dong, Libing; Zhao, Jian-Kang; Hu, Xiaofang; Shen, Chaohua; Qiao, Yuxin; Zhang, Xinyue; Wang, Yapei; Ismagilov, Rustem F; Liu, Shuang-Jiang; Du, Wenbin

    2016-04-01

    This paper describes the microfluidic streak plate (MSP), a facile method for high-throughput microbial cell separation and cultivation in nanoliter sessile droplets. The MSP method builds upon the conventional streak plate technique by using microfluidic devices to generate nanoliter droplets that can be streaked manually or robotically onto petri dishes prefilled with carrier oil for cultivation of single cells. In addition, chemical gradients could be encoded in the droplet array for comprehensive dose-response analysis. The MSP method was validated by using single-cell isolation of Escherichia coli and antimicrobial susceptibility testing of Pseudomonas aeruginosa PAO1. The robustness of the MSP work flow was demonstrated by cultivating a soil community that degrades polycyclic aromatic hydrocarbons. Cultivation in droplets enabled detection of the richest species diversity with better coverage of rare species. Moreover, isolation and cultivation of bacterial strains by MSP led to the discovery of several species with high degradation efficiency, including four Mycobacterium isolates and a previously unknown fluoranthene-degrading Blastococcus species. PMID:26850294

  10. Attosecond streaking of molecules in the low-energy region studied by a wavefunction splitting scheme

    NASA Astrophysics Data System (ADS)

    Hou, Xue-Feng; Peng, Liang-You; Ning, Qi-Cheng; Gong, Qihuang

    2012-04-01

    We present a theoretical study of the low-energy photoelectron spectra of hydrogen molecular ion generated by a single attosecond pulse in the presence of an infrared (IR) laser field. In order to investigate this type of attosecond streaking of molecules, we developed a very efficient grid-based numerical method to solve the two-centre time-dependent Schrödinger equation (TDSE) in the prolate spheroidal coordinates. Specifically, the radial coordinate is discretized with the finite-element discrete variable representation (FE-DVR) for easy parallel computation and the angular coordinate with the usual DVR. A wavefunction splitting scheme is utilized to reduce the demanding requirement of the computational resource to solve the corresponding TDSE when an IR field is present. After verification of the accuracy and efficiency of our method, we then apply it to investigate the attosecond streaking spectra of H+2 in the low-energy region. In contrast to the usual attosecond streaking in the high-energy region, part of the low-energy electrons may be driven back to rescatter with the residual two-centre core. Very interesting interference structures are present in the low-energy region. When the internuclear distance is small, they are very similar to what we have recently observed in the atomic case.

  11. Optical fiducial timing system for X-ray streak cameras with aluminum coated optical fiber ends

    DOEpatents

    Nilson, David G.; Campbell, E. Michael; MacGowan, Brian J.; Medecki, Hector

    1988-01-01

    An optical fiducial timing system is provided for use with interdependent groups of X-ray streak cameras (18). The aluminum coated (80) ends of optical fibers (78) are positioned with the photocathodes (20, 60, 70) of the X-ray streak cameras (18). The other ends of the optical fibers (78) are placed together in a bundled array (90). A fiducial optical signal (96), that is comprised of 2.omega. or 1.omega. laser light, after introduction to the bundled array (90), travels to the aluminum coated (82) optical fiber ends and ejects quantities of electrons (84) that are recorded on the data recording media (52) of the X-ray streak cameras (18). Since both 2.omega. and 1.omega. laser light can travel long distances in optical fiber with only a slight attenuation, the initial arial power density of the fiducial optical signal (96) is well below the damage threshold of the fused silica or other material that comprises the optical fibers (78, 90). Thus the fiducial timing system can be repeatably used over long durations of time.

  12. Altitude Starting Characteristics of an Afterburner with Autoignition and Hot-streak Ignition

    NASA Technical Reports Server (NTRS)

    Renas, P E; Jansen, E T; Harvey, R W , Sr

    1953-01-01

    An investigation was conducted in an altitude test chamber at the NACA Lewis Laboratory to determine the altitude starting characteristics of an afterburner with autoignition and with hot-streak ignition. Transient afterburner ignition data were obtained over a range of altitudes from 30,000 to 50,000 feet at a flight Mach number of 0.60. Afterburner ignition with a torch igniter located axially at approximately the midpoint of the combustion chamber was possible over the entire range, but ignition ignition with a torch igniter located in the transition section 1 5/8 inches upstream of the turbine stators proved unsatisfactory at an altitude of 50,000 feet due to the inability to obtain flame through the turbine. Increasing the afterburner-inlet total pressure at a constant afterburner fuel-air ratio decreased the afterburner ignition time. Hot-streak ignition was possible within 2 seconds after the time required to obtain the preset, normal afterburner fuel pressure, whereas autoignition required 4 to 7 seconds for the range of altitudes investigated. Following the ignition there was a period of oscillatory operation existing in the engine-afterburner before steady-state operation was attained. The time required for steady-state stable operation decreased as afterburner inlet total pressure increased. The duration of oscillations also decreased with hot-streak ignition because the fuel-air mixture was ignited before a large volume of combustible mixture was accumulated in the afterburner.

  13. Unsteady numerical simulation of hot streak/blades interaction and film cooling

    NASA Astrophysics Data System (ADS)

    Yi, Weilin; Ji, Lucheng; Xiao, Yunhan

    2010-10-01

    Deeply research on management and application of hot streak is an important way to breakthrough technique obstacle of aero engine hot components. Numerical method is a useful instrument to investigate the correlative problems. Firstly the paper developed independently three dimensional unsteady parallel computational code-MpiTurbo based on Fortran 90 and MPI at Linux operating system. Then unsteady numerical simulation was carried out to investigate impacts of the factors, which included circumferential locations of hot streak and clocking positions of blade rows, on the thermal environment of a 1+1 counter-rotating turbine. The results clearly indicated that clocking positions of hot streak/blade row and blade row/blade row had great influence on the time-averaged temperature distribution of the third blade row. Therefore, it can be effective for improving thermal environment of turbine to optimize blade parameters and clocking positions. Lastly film cooling layout was designed by the repetitious steady simulation based on source term method. And the flow structure detail was given by the unsteady simulation.

  14. Laser Activated Streak Camera for Measurement of Electron Pulses with Femtosecond Resolution

    NASA Astrophysics Data System (ADS)

    Zandi, Omid; Desimone, Alice; Wilkin, Kyle; Yang, Jie; Centurion, Martin

    2015-05-01

    The duration of femtosecond electron pulses used in time-resolved diffraction and microscopy experiments is challenging to measure in-situ. To overcome this problem, we have fabricated a streak camera that uses the time-varying electric field of a discharging parallel plate capacitor. The capacitor is discharged using a laser-activated GaAs photoswitch, resulting in a damped oscillation of the electric field. The delay time between the laser pulse and electron pulse is set so that the front and back halves of the bunch encounter opposite electric fields of the capacitor and are deflected in opposite directions. Thus, the electron bunch appears streaked on the detector with a length proportional to its duration. The temporal resolution of the streak camera is proportional to the maximum value of the electric field and the frequency of the discharge oscillation. The capacitor is charged by high voltage short pulses to achieve a high electric field and prevent breakdown. We have achieved an oscillation frequency in the GHz range by reducing the circuit size and hence its inductance. The camera was used to measure 100 keV electron pulses with up to a million electrons that are compressed transversely by magnetic lenses and longitudinally by an RF cavity. This work was supported mainly by the Air Force Office of Scientific Research, Ultrashort Pulse Laser Matter Interaction program, under grant # FA9550-12-1-0149.

  15. The Dynamics and Environmental Influence on Interactions Between Cassava Brown Streak Disease and the Whitefly, Bemisia tabaci.

    PubMed

    Jeremiah, S C; Ndyetabula, I L; Mkamilo, G S; Haji, S; Muhanna, M M; Chuwa, C; Kasele, S; Bouwmeester, H; Ijumba, J N; Legg, J P

    2015-05-01

    Cassava brown streak disease (CBSD) is currently the most significant virus disease phenomenon affecting African agriculture. In this study, we report results from the most extensive set of field data so far presented for CBSD in Africa. From assessments of 515 farmers' plantings of cassava, incidence in the Coastal Zone of Tanzania (46.5% of plants; 87% of fields affected) was higher than in the Lake Zone (22%; 34%), but incidences for both zones were greater than previous published records. The whitefly vector, Bemisia tabaci, was more abundant in the Lake Zone than the Coastal Zone, the reverse of the situation reported previously, and increased B. tabaci abundance is driving CBSD spread in the Lake Zone. The altitudinal "ceiling" previously thought to restrict the occurrence of CBSD to regions <1,000 masl has been broken as a consequence of the greatly increased abundance of B. tabaci in mid-altitude areas. Among environmental variables analyzed, minimum temperature was the strongest determinant of CBSD incidence. B. tabaci in the Coastal and Lake Zones responded differently to environmental variables examined, highlighting the biological differences between B. tabaci genotypes occurring in these regions and the superior adaptation of B. tabaci in the Great Lakes region both to cassava and low temperature conditions. Regression analyses using multi-country data sets could be used to determine the potential environmental limits of CBSD. Approaches such as this offer potential for use in the development of predictive models for CBSD, which could strengthen country- and continent-level CBSD pandemic mitigation strategies. PMID:25585059

  16. Low Albedo Surfaces and Eolian Sediment: Mars Orbiter Camera Views of Western Arabia Terra Craters and Wind Streaks

    NASA Technical Reports Server (NTRS)

    Edgett, Kenneth S.

    2001-01-01

    High spatial resolution (1.5 to 12 m/pixel) Mars Global Surveyor Mars Orbiter Camera images obtained September 1997 through June 2001 indicate that the large, dark wind streaks of western Arabia Terra each originate at a barchan dune field on a crater floor. The streaks consist of a relatively thin coating of sediment deflated from the dune fields and their vicinity. This sediment drapes a previous mantle that more thickly covers nearly all of western Arabia Terra. No dunes or eolian bedforms are found within the dark wind streaks, nor do any of the intracrater dunes climb up crater walls to provide sand to the wind streaks. The relations between dunes, wind streak, and subjacent terrain imply that dark-toned grains finer than those which comprise the dunes are lifted into suspension and carried out of the craters to be deposited on the adjacent terrain. Such grains are most likely in the silt size range (3.9-62.5 micrometers). The streaks change in terms of extent, relative albedo, and surface pattern over periods measured in years, but very little evidence for recent eolian activity (dust plumes, storms, dune movement) has been observed.

  17. Effects of replacing conventional corn silage with BMR corn silage

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Previous research has shown that the (lignin reducing) brown mid-rib mutation in corn silage, which increases in vitro fiber digestibility, does not always improve fiber digestibility when fed as part of a TMR; however, feed intake and milk production are increased. The objectives of this experiment...

  18. Dispersal behavior of neonate European corn borer (Lepidoptera: Crambidae) on Bt corn.

    PubMed

    Razze, J M; Mason, C E

    2012-08-01

    European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), has historically been a significant economically important insect pest of corn (Zea mays L.) in the United States and Canada. The development in the 1990s of genetically modified corn expressing genes derived from Bacillus thuringiensis (Bt) that encodes insecticidal crystalline (Cry) proteins has proven to be effective in controlling this insect as well as other corn pests. The purpose of this study was to assess the movement and dispersal behavior of neonate European corn borer on Bt corn. We examined differences in neonate European corn borer dispersal behavior for the first 4 h after eclosion in the field among a stacked pyramid (Cry1F X Cry1Ab X Cry34/35Ab1) Bt corn, a Cry1F Bt corn, and a non-Bt sweet corn; and in the laboratory among a Bt corn hybrid containing Cry1F, a hybrid containing Cry1Ab, a pyramid combining these two hybrids (Cry1F X Cry1Ab), and a non-Bt near isoline corn. In field experiments, we found that dispersal was significantly higher on Bt corn compared with sweet corn. In laboratory experiments, dispersal was significantly higher on Cry1Ab Bt corn and Cry1F X Cry1Ab Bt corn than on non-Bt near isoline corn. Results indicated that neonate dispersal may be significantly greater in Bt cornfields compared with non-Bt cornfields. The findings on dispersal behavior in this study will be useful in evaluating the efficacy of a blended seed refuge system for managing European corn borer resistance in Bt corn. PMID:22928300

  19. Compatibility with corn: N credits

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Productive and efficient short rotations of alfalfa and corn are needed to reduce energy inputs, produce food, feed, and energy, and yield the environmental quality benefits from the perennial legume. After decades of research, however, farmers and their advisors still question how much fertility ...

  20. Sustainable Corn Stover Harvest Strategies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn stover has been identified as an important initial source of biomass for conversion to ethanol and other biofuels. This poster presentation outlines on-going cooperative research being conducted near Ames, IA. Our university partner is responsible for developing the one-pass harvester and our I...

  1. Corn gluten meal application equipment

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Previous research has determined that corn gluten meal (CGM) produces an inhibitory effect and reduces root formation in several weed species. One limitation to further evaluation of CGM in field vegetable production is the difficulty in achieving a uniform application to the soil surface. The use o...

  2. Improved corn protein based articles

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Developing higher value uses for zein (corn protein), a potential major co-product of the bio-ethanol industry, will improve the economics of this business. Historically, zein was predominantly used in the textile fiber industry. Unfortunately the techniques used at that time to modify the zein cann...

  3. Our Mother Corn. Teacher's Guide.

    ERIC Educational Resources Information Center

    Mathers, Sherry; And Others

    Designed to accompany the preceding student text (which deals with the role of corn in the Seneca, Pawnee, and Hopi tribes), the teaching guide contains a suggested sequence of activities and needed supplementary information along with an indication of the student text they follow. Sections include: farming notes; basic needs activities; house…

  4. Influence of corn oil recovery on life-cycle greenhouse gas emissions of corn ethanol and corn oil biodiesel

    DOE PAGESBeta

    Wang, Zhichao; Dunn, Jennifer B.; Han, Jeongwoo; Wang, Michael

    2015-11-04

    Corn oil recovery and conversion to biodiesel has been widely adopted at corn ethanol plants recently. The US EPA has projected 2.6 billion liters of biodiesel will be produced from corn oil in 2022. Corn oil biodiesel may qualify for federal renewable identification number (RIN) credits under the Renewable Fuel Standard, as well as for low greenhouse gas (GHG) emission intensity credits under California’s Low Carbon Fuel Standard. Because multiple products [ethanol, biodiesel, and distiller’s grain with solubles (DGS)] are produced from one feedstock (corn), however, a careful co-product treatment approach is required to accurately estimate GHG intensities of bothmore » ethanol and corn oil biodiesel and to avoid double counting of benefits associated with corn oil biodiesel production. This study develops four co-product treatment methods: (1) displacement, (2) marginal, (3) hybrid allocation, and (4) process-level energy allocation. Life-cycle GHG emissions for corn oil biodiesel were more sensitive to the choice of co-product allocation method because significantly less corn oil biodiesel is produced than corn ethanol at a dry mill. Corn ethanol life-cycle GHG emissions with the displacement, marginal, and hybrid allocation approaches are similar (61, 62, and 59 g CO2e/MJ, respectively). Although corn ethanol and DGS share upstream farming and conversion burdens in both the hybrid and process-level energy allocation methods, DGS bears a higher burden in the latter because it has lower energy content per selling price as compared to corn ethanol. As a result, with the process-level allocation approach, ethanol’s life-cycle GHG emissions are lower at 46 g CO2e/MJ. Corn oil biodiesel life-cycle GHG emissions from the marginal, hybrid allocation, and process-level energy allocation methods were 14, 59, and 45 g CO2e/MJ, respectively. Sensitivity analyses were conducted to investigate the influence corn oil yield, soy biodiesel, and defatted DGS displacement

  5. Influence of corn oil recovery on life-cycle greenhouse gas emissions of corn ethanol and corn oil biodiesel

    SciTech Connect

    Wang, Zhichao; Dunn, Jennifer B.; Han, Jeongwoo; Wang, Michael

    2015-11-04

    Corn oil recovery and conversion to biodiesel has been widely adopted at corn ethanol plants recently. The US EPA has projected 2.6 billion liters of biodiesel will be produced from corn oil in 2022. Corn oil biodiesel may qualify for federal renewable identification number (RIN) credits under the Renewable Fuel Standard, as well as for low greenhouse gas (GHG) emission intensity credits under California’s Low Carbon Fuel Standard. Because multiple products [ethanol, biodiesel, and distiller’s grain with solubles (DGS)] are produced from one feedstock (corn), however, a careful co-product treatment approach is required to accurately estimate GHG intensities of both ethanol and corn oil biodiesel and to avoid double counting of benefits associated with corn oil biodiesel production. This study develops four co-product treatment methods: (1) displacement, (2) marginal, (3) hybrid allocation, and (4) process-level energy allocation. Life-cycle GHG emissions for corn oil biodiesel were more sensitive to the choice of co-product allocation method because significantly less corn oil biodiesel is produced than corn ethanol at a dry mill. Corn ethanol life-cycle GHG emissions with the displacement, marginal, and hybrid allocation approaches are similar (61, 62, and 59 g CO2e/MJ, respectively). Although corn ethanol and DGS share upstream farming and conversion burdens in both the hybrid and process-level energy allocation methods, DGS bears a higher burden in the latter because it has lower energy content per selling price as compared to corn ethanol. As a result, with the process-level allocation approach, ethanol’s life-cycle GHG emissions are lower at 46 g CO2e/MJ. Corn oil biodiesel life-cycle GHG emissions from the marginal, hybrid allocation, and process-level energy allocation methods were 14, 59, and 45 g CO2e/MJ, respectively. Sensitivity analyses were conducted to investigate the influence corn oil yield, soy biodiesel, and

  6. Measuring 8–250 ps short pulses using a high-speed streak camera on kilojoule, petawatt-class laser systems

    SciTech Connect

    Qiao, J.; Jaanimagi, P. A.; Boni, R.; Bromage, J.; Hill, E.

    2013-07-15

    Short-pulse measurements using a streak camera are sensitive to space-charge broadening, which depends on the pulse duration and shape, and on the uniformity of photocathode illumination. An anamorphic-diffuser-based beam-homogenizing system and a space-charge-broadening calibration method were developed to accurately measure short pulses using an optical streak camera. This approach provides a more-uniform streak image and enables one to characterize space-charge-induced pulse-broadening effects.

  7. Synchronous time-resolved optical and x-ray emission from simultaneous optical and x-ray streak cameras driven by a master ramp generator

    SciTech Connect

    Balmer, J.E.; Lampert, W.; Roschger, E.; Hares, J.D.; Kilkenny, J.D.

    1985-05-01

    An optical and an x-ray streak camera have been synchronized by driving the deflection plates of both cameras from the same ramp generator. The relative timing of the two cameras was calibrated by running UV light onto the x-ray streak camera. The x-ray streak camera was then used to measure the time of the x-ray emission from a laser plasma with respect to the laser pulse.

  8. A generic method to identify plant viruses by high-resolution tandem mass spectrometry of their coat proteins.

    PubMed

    Blouin, Arnaud G; Greenwood, David R; Chavan, Ramesh R; Pearson, Michael N; Clover, Gerard R G; MacDiarmid, Robin M; Cohen, Daniel

    2010-01-01

    Although a number of protocols have been developed for detection of viruses at the genus or family level, universal approaches to detect and identify unknown viruses are still required. High-resolution tandem mass spectrometry was used to identify accurately peptide masses and their constituent sequences from partially purified plant virus preparations. Analysis of the peptide fragment masses against a virus database using pattern-matching algorithms identified sequences with homology to known virus peptides and also predicted peptides using de novo sequence analysis. This method provided sufficient information to confirm the identity of two known viruses that were included as controls (Cucumber mosaic virus and Tomato spotted wilt virus) and to identify unknown viruses in six viral isolates. The unknown viruses have been identified as four common viruses (Alfalfa mosaic virus, Tobacco streak virus, Citrus leaf blotch virus and Ribgrass mosaic virus), and two novel viruses (a potexvirus and a vitivirus). The identification of viruses from five distinct families by the tandem mass spectrometric determination of their coat protein demonstrates that this is a useful method for initial virus identification. This method, complemented with molecular or immunological procedures, provides a rapid and convenient way to identify both known and novel plant viruses. PMID:19712699

  9. 21 CFR 137.215 - Yellow corn flour.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Yellow corn flour. 137.215 Section 137.215 Food... Flours and Related Products § 137.215 Yellow corn flour. Yellow corn flour conforms to the definition and standard of identity prescribed by § 137.211 for white corn flour except that cleaned yellow corn is...

  10. 7 CFR 810.401 - Definition of corn.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Definition of corn. 810.401 Section 810.401... GRAIN United States Standards for Corn Terms Defined § 810.401 Definition of corn. Grain that consists of 50 percent or more of whole kernels of shelled dent corn and/or shelled flint corn (Zea mays...

  11. 21 CFR 137.275 - Yellow corn meal.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Yellow corn meal. 137.275 Section 137.275 Food and... Related Products § 137.275 Yellow corn meal. Yellow corn meal conforms to the definition and standard of identity prescribed by § 137.250 for white corn meal except that cleaned yellow corn is used instead...

  12. 21 CFR 137.275 - Yellow corn meal.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Yellow corn meal. 137.275 Section 137.275 Food and... Related Products § 137.275 Yellow corn meal. Yellow corn meal conforms to the definition and standard of identity prescribed by § 137.250 for white corn meal except that cleaned yellow corn is used instead...

  13. 21 CFR 137.265 - Degerminated white corn meal.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Degerminated white corn meal. 137.265 Section 137... Cereal Flours and Related Products § 137.265 Degerminated white corn meal. (a) Degerminated white corn meal, degermed white corn meal, is the food prepared by grinding cleaned white corn and removing...

  14. 7 CFR 810.401 - Definition of corn.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Definition of corn. 810.401 Section 810.401... GRAIN United States Standards for Corn Terms Defined § 810.401 Definition of corn. Grain that consists of 50 percent or more of whole kernels of shelled dent corn and/or shelled flint corn (Zea mays...

  15. 21 CFR 137.265 - Degerminated white corn meal.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Degerminated white corn meal. 137.265 Section 137... Cereal Flours and Related Products § 137.265 Degerminated white corn meal. (a) Degerminated white corn meal, degermed white corn meal, is the food prepared by grinding cleaned white corn and removing...

  16. 21 CFR 137.215 - Yellow corn flour.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Yellow corn flour. 137.215 Section 137.215 Food... Flours and Related Products § 137.215 Yellow corn flour. Yellow corn flour conforms to the definition and standard of identity prescribed by § 137.211 for white corn flour except that cleaned yellow corn is...

  17. 7 CFR 810.401 - Definition of corn.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Definition of corn. 810.401 Section 810.401... GRAIN United States Standards for Corn Terms Defined § 810.401 Definition of corn. Grain that consists of 50 percent or more of whole kernels of shelled dent corn and/or shelled flint corn (Zea mays...

  18. 21 CFR 137.265 - Degerminated white corn meal.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Degerminated white corn meal. 137.265 Section 137... Cereal Flours and Related Products § 137.265 Degerminated white corn meal. (a) Degerminated white corn meal, degermed white corn meal, is the food prepared by grinding cleaned white corn and removing...

  19. 21 CFR 137.275 - Yellow corn meal.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Yellow corn meal. 137.275 Section 137.275 Food and... Related Products § 137.275 Yellow corn meal. Yellow corn meal conforms to the definition and standard of identity prescribed by § 137.250 for white corn meal except that cleaned yellow corn is used instead...

  20. 21 CFR 137.215 - Yellow corn flour.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Yellow corn flour. 137.215 Section 137.215 Food... Flours and Related Products § 137.215 Yellow corn flour. Yellow corn flour conforms to the definition and standard of identity prescribed by § 137.211 for white corn flour except that cleaned yellow corn is...

  1. 21 CFR 137.265 - Degerminated white corn meal.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Degerminated white corn meal. 137.265 Section 137... Cereal Flours and Related Products § 137.265 Degerminated white corn meal. (a) Degerminated white corn meal, degermed white corn meal, is the food prepared by grinding cleaned white corn and removing...

  2. 7 CFR 810.401 - Definition of corn.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 7 2013-01-01 2013-01-01 false Definition of corn. 810.401 Section 810.401... GRAIN United States Standards for Corn Terms Defined § 810.401 Definition of corn. Grain that consists of 50 percent or more of whole kernels of shelled dent corn and/or shelled flint corn (Zea mays...

  3. 21 CFR 137.265 - Degerminated white corn meal.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Degerminated white corn meal. 137.265 Section 137... Cereal Flours and Related Products § 137.265 Degerminated white corn meal. (a) Degerminated white corn meal, degermed white corn meal, is the food prepared by grinding cleaned white corn and removing...

  4. 21 CFR 137.215 - Yellow corn flour.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Yellow corn flour. 137.215 Section 137.215 Food... Flours and Related Products § 137.215 Yellow corn flour. Yellow corn flour conforms to the definition and standard of identity prescribed by § 137.211 for white corn flour except that cleaned yellow corn is...

  5. 21 CFR 137.275 - Yellow corn meal.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Yellow corn meal. 137.275 Section 137.275 Food and... Related Products § 137.275 Yellow corn meal. Yellow corn meal conforms to the definition and standard of identity prescribed by § 137.250 for white corn meal except that cleaned yellow corn is used instead...

  6. 21 CFR 137.215 - Yellow corn flour.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Yellow corn flour. 137.215 Section 137.215 Food... Flours and Related Products § 137.215 Yellow corn flour. Yellow corn flour conforms to the definition and standard of identity prescribed by § 137.211 for white corn flour except that cleaned yellow corn is...

  7. 21 CFR 137.275 - Yellow corn meal.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Yellow corn meal. 137.275 Section 137.275 Food and... Related Products § 137.275 Yellow corn meal. Yellow corn meal conforms to the definition and standard of identity prescribed by § 137.250 for white corn meal except that cleaned yellow corn is used instead...

  8. 7 CFR 810.401 - Definition of corn.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Definition of corn. 810.401 Section 810.401... GRAIN United States Standards for Corn Terms Defined § 810.401 Definition of corn. Grain that consists of 50 percent or more of whole kernels of shelled dent corn and/or shelled flint corn (Zea mays...

  9. Pest Control in Corn and Soybeans: Weeds - Insects - Diseases.

    ERIC Educational Resources Information Center

    Doersch, R. E.; And Others

    This document gives the characteristics and application rates for herbicides used to control annual weeds in corn, annual and perennial broadleaf weeds in corn, quackgrass and yellow nutsedge in corn, and annual weeds in soybeans. It also gives insecticide use information for corn and soybeans. A brief discussion of disease control in corn and…

  10. 75 FR 48321 - Corning Natural Gas Corporation; Notice of Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-10

    ... Energy Regulatory Commission Corning Natural Gas Corporation; Notice of Application August 4, 2010. Take notice that on July 26, 2010, Corning Natural Gas Corporation (Corning), 330 W. William Street, Corning... Natural Gas Act (NGA) requesting the determination of a service area with which Corning may,...

  11. Absolute calibration method for nanosecond-resolved, time-streaked, fiber optic light collection, spectroscopy systems

    SciTech Connect

    Johnston, Mark D.; Oliver, Bryan V.; Droemer, Darryl W.; Frogget, Brent; Crain, Marlon D.; Maron, Yitzhak

    2012-08-15

    This paper describes a convenient and accurate method to calibrate fast (<1 ns resolution) streaked, fiber optic light collection, spectroscopy systems. Such systems are inherently difficult to calibrate due to the lack of sufficiently intense, calibrated light sources. Such a system is used to collect spectral data on plasmas generated in electron beam diodes fielded on the RITS-6 accelerator (8-12MV, 140-200kA) at Sandia National Laboratories. On RITS, plasma light is collected through a small diameter (200 {mu}m) optical fiber and recorded on a fast streak camera at the output of a 1 meter Czerny-Turner monochromator. For this paper, a 300 W xenon short arc lamp (Oriel Model 6258) was used as the calibration source. Since the radiance of the xenon arc varies from cathode to anode, just the area around the tip of the cathode ('hotspot') was imaged onto the fiber, to produce the highest intensity output. To compensate for chromatic aberrations, the signal was optimized at each wavelength measured. Output power was measured using 10 nm bandpass interference filters and a calibrated photodetector. These measurements give power at discrete wavelengths across the spectrum, and when linearly interpolated, provide a calibration curve for the lamp. The shape of the spectrum is determined by the collective response of the optics, monochromator, and streak tube across the spectral region of interest. The ratio of the spectral curve to the measured bandpass filter curve at each wavelength produces a correction factor (Q) curve. This curve is then applied to the experimental data and the resultant spectra are given in absolute intensity units (photons/sec/cm{sup 2}/steradian/nm). Error analysis shows this method to be accurate to within +/- 20%, which represents a high level of accuracy for this type of measurement.

  12. Significant seismic streaks corresponding to lithological contrasts in Mesozoic and Paleozoic accretion units, southwest Japan

    NASA Astrophysics Data System (ADS)

    Maeda, S.; Toda, S.; Katao, H.

    2014-12-01

    While high-density seismic streaks are commonly seen in aftershocks of a strike-slip earthquake, significant linear clusters in background seismicity are rare. A typical example of such sustained streaks is observed along the creeping section of the San Andreas fault, in which strain localization associated with frictional heterogeneity takes a responsibility. Here we show other examples from Tamba and Wakayama regions, around Osaka-Kyoto district, where a number of several-to-20-km-long seismic streaks are observed. We explore the role of geologic heterogeneity into the seismicity comparing spatial distribution of earthquakes with geologic structure in both regions, where a significant high background rate of seismicity has been continuously recorded since the mid-1900. Epicenters of numerous small earthquakes are located mainly on the Mesozoic metamorphic rocks and Mesozoic-Paleozoic accretion units, whereas low seismicity is characterized in granite and ultra-mafic rocks. Within the Wakayama seismic zone, in particular, we observe many E-W and ENE-WSW trending dense seismic clusters in hypocenters of Japan Meteorological Agency (JMA) data relocated with the hypo DD algorithm of Waldhauser and Ellsworth (2000). Most of the E-W trending seismic clusters possibly correspond to the E-W trending local scale geologic faults, folds, bedding planes, and schistosity. However, well-determined fault plane solutions by JMA and the National Research Institute for Earth Science and Disaster Prevention (NIED) show NS-trending reverse faults corresponding to EW compression. We further sought the focal mechanisms for smaller earthquakes using waveform data recorded in the SATARN seismic network system of DPRI, Kyoto University. As a result, among the many reverse fault mechanisms, we found some amounts of strike-slip ones, which may associate with the visible EW-trending seismic clusters.

  13. Absolute calibration method for nanosecond-resolved, time-streaked, fiber optic light collection, spectroscopy systems

    NASA Astrophysics Data System (ADS)

    Johnston, Mark D.; Oliver, Bryan V.; Droemer, Darryl W.; Frogget, Brent; Crain, Marlon D.; Maron, Yitzhak

    2012-08-01

    This paper describes a convenient and accurate method to calibrate fast (<1 ns resolution) streaked, fiber optic light collection, spectroscopy systems. Such systems are inherently difficult to calibrate due to the lack of sufficiently intense, calibrated light sources. Such a system is used to collect spectral data on plasmas generated in electron beam diodes fielded on the RITS-6 accelerator (8-12MV, 140-200kA) at Sandia National Laboratories. On RITS, plasma light is collected through a small diameter (200 μm) optical fiber and recorded on a fast streak camera at the output of a 1 meter Czerny-Turner monochromator. For this paper, a 300 W xenon short arc lamp (Oriel Model 6258) was used as the calibration source. Since the radiance of the xenon arc varies from cathode to anode, just the area around the tip of the cathode ("hotspot") was imaged onto the fiber, to produce the highest intensity output. To compensate for chromatic aberrations, the signal was optimized at each wavelength measured. Output power was measured using 10 nm bandpass interference filters and a calibrated photodetector. These measurements give power at discrete wavelengths across the spectrum, and when linearly interpolated, provide a calibration curve for the lamp. The shape of the spectrum is determined by the collective response of the optics, monochromator, and streak tube across the spectral region of interest. The ratio of the spectral curve to the measured bandpass filter curve at each wavelength produces a correction factor (Q) curve. This curve is then applied to the experimental data and the resultant spectra are given in absolute intensity units (photons/sec/cm2/steradian/nm). Error analysis shows this method to be accurate to within +/- 20%, which represents a high level of accuracy for this type of measurement.

  14. A Dust Devil Making a Streak and Climbing a Crater Wall

    NASA Technical Reports Server (NTRS)

    2002-01-01

    MGS MOC Release No. MOC2-318, 8 August 2002 [figure removed for brevity, see original site] One of the key elements of the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) Extended Mission is to look for and monitor changes taking place on the planet over the course of a second--and, eventually, a third--martian year. MGS is now well into its second Mars year, which will draw to a close in December 2002. Among the changes the MOC has observed are streaks believed to be caused by the passage of dust devils. Thousands of MOC images show these streaks, dozens show that they change over time, but far fewer images have actually captured a dust devil in the act of creating a streak. At the center right of this image (above left) is a dust devil that, on May 21, 2002, was seen climbing the wall of a crater at 4.1oS, 9.5oW. This crater (above right) is in western Terra Meridiani. The dust devil was moving toward the northeast (upper right), leaving behind a dark trail where a thin coating of surficial dust was removed or disrupted as the dust devil advanced. Dust devils most commonly form after noon on days when the martian air is still (that is, when there isn't even a faint breeze). On such days, the ground is better able to heat up the air immediately above the surface. As the warmed near-surface air begins to rise, it also begins to spin, creating a vortex. The spinning column then moves across the surface and picks up loose dust (if any is present). The dust makes the vortex visible and gives it a tornado-like appearance. The dust devil in this image has a very short, dark shadow cast to the right of the bright column; this shadow is short because the sun was nearly overhead.

  15. Effect of Amirkabiria odorastissima mozaffarian on the development and progression of fatty streaks in hypercholesterolemic rabbits.

    PubMed

    Asgary, S; Naderi, Gh; Dashti, Gh; Paknahad, Z

    2004-05-01

    New studies have introduced atherosclerosis as an inflammatory disease. Different factors affect the development of inflammation that can be linked to free radicals, hypertention, diabetes mellitus, genetic changes, hypercholesterolemia and some of the microorganisms. Using anti-inflammatory drugs can be useful in preventing atherosclerosis. Finding drugs from natural sources and without side-effects can be useful. Amirkabiria odoratissima (umbelliferae) is from Iranian fl ora and is used traditionally as an anti-inflammatory drug. This study was designed to assess the effects of this plant on the development and progression of fatty streaks. Male rabbits were fed: (a) rabbit chow; (b) 1% cholesterol diet; (c) cholesterol diet supplemented with the plant; (d) normal diet supplemented with the plant. After 12 weeks, the animals were sacrificed and the aorta, right and left branches of coronary arteries were dissected and histologically processed. Before and after the end of the study, biochemical factors were measured. The amounts of cholesterol, LDL, HDL, triglyceride and fasting blood sugar (FBS) were determined by enzymatic methods, quantitative CRP was determined by turbidimetric methods, malondialdehyde (MDA) and antioxidant capacity were determined by speceterophotometric methods. The results indicate that there is a significant difference between the groups supplemented with herbal medicine and others (p < 0.05) in the mean grade of fatty streak of right and left coronary artery and aorta. At the end of the 12-week period cholesterol, LDL and CRP were significantly reduced in the groups which received herbal medicine (p < 0.05). The data suggests that Amirkabiria odoratissima Mozaffarian has beneficial effects to prevent development of fatty streak; however, further studies are needed to understand the mechanisms whereby this plant exerts its anti-atherosclerotic effects. PMID:15173995

  16. Direct Shock-Timing Measurements in CH Using Streaked X-Ray Radiography

    NASA Astrophysics Data System (ADS)

    Nilson, P. M.; Lafon, M.; Stillman, C. R.; Mileham, C.; Boni, R.; Boehly, T. R.; Froula, D. H.; Meyerhofer, D. D.

    2014-10-01

    One-dimensional streaked x-ray radiography is used to measure shock coalescence in multishocked plastic. A two-shock system was generated using a ramped drive on the OMEGA EP Laser System. The data show the first shock wave propagating into solid material, followed 2 ns later by the second shock wave. The measured shock trajectories were used to track the system dynamics and determine the shock-coalescence times for different initial shock strengths. The measured shock timings are compared to radiation-hydrodynamic model predictions. This material is based upon work supported by the Department of Energy National Nuclear Security Administration under Award Number DE-NA0001944.

  17. Physics and roller coasters-The Blue Streak at Cedar Point

    NASA Astrophysics Data System (ADS)

    Speers, Robert R.

    1991-06-01

    The use of a roller coaster for external classroom studies of kinematics, forces, dynamics, and energy conservation is discussed. Experimental accelerometer measurements of the vertical forces acting on riders of the Blue Streak at Cedar Point in Sandusky, Ohio are presented. Theoretically, the track profile is used to calculate/predict the vertical forces acting on a rider at the hilltops, valley bottoms, and several other points of interest along the track. Finally the experimental results and theoretical predictions are compared in the context of a rider's experiences.

  18. Capsule Ablator Inflight Performance Measurements Via Streaked Radiography Of ICF Implosions On The NIF*

    NASA Astrophysics Data System (ADS)

    Dewald, E. L.; Tommasini, R.; Mackinnon, A.; MacPhee, A.; Meezan, N.; Olson, R.; Hicks, D.; LePape, S.; Izumi, N.; Fournier, K.; Barrios, M. A.; Ross, S.; Pak, A.; Döppner, T.; Kalantar, D.; Opachich, K.; Rygg, R.; Bradley, D.; Bell, P.; Hamza, A.; Dzenitis, B.; Landen, O. L.; MacGowan, B.; LaFortune, K.; Widmayer, C.; Van Wonterghem, B.; Kilkenny, J.; Edwards, M. J.; Atherton, J.; Moses, E. I.

    2016-03-01

    Streaked 1-dimensional (slit imaging) radiography of 1.1 mm radius capsules in ignition hohlraums was recently introduced on the National Ignition Facility (NIF) and gives an inflight continuous record of capsule ablator implosion velocities, shell thickness and remaining mass in the last 3-5 ns before peak implosion time. The high quality data delivers good accuracy in implosion metrics that meets our requirements for ignition and agrees with recently introduced 2-dimensional pinhole radiography. Calculations match measured trajectory across various capsule designs and laser drives when the peak laser power is reduced by 20%. Furthermore, calculations matching measured trajectories give also good agreement in ablator shell thickness and remaining mass.

  19. Temporal Characterization of Electron Beam Bunches with a Fast Streak Camera at the JLab FEL Facility

    SciTech Connect

    S. Zhang; S.V. Benson; D. Douglas; D. Hardy; C. Hernandez-Garcia; K. Jordan; G. Neil; Michelle D. Shinn

    2005-08-21

    The design and construction of an optical transport that brings synchrotron radiation from electron bunches to a fast streak camera in a remote area has become a useful tool for online observation of bunch length and stability. This paper will report on the temporal measurements we have done, comparison with simulations, and the on-going work for another imaging optical transport system that will make possible the direct measurement of the longitudinal phase space by measuring the bunch length as a function of energy

  20. Transient electric fields in laser plasmas observed by proton streak deflectometry

    SciTech Connect

    Sokollik, T.; Schnuerer, M.; Ter-Avetisyan, S.; Nickles, P. V.; Risse, E.; Kalashnikov, M.; Sandner, W.; Priebe, G.; Amin, M.; Toncian, T.; Willi, O.; Andreev, A. A.

    2008-03-03

    A novel proton imaging technique was applied which allows a continuous temporal record of electric fields within a time window of several nanoseconds. This 'proton streak deflectometry' was used to investigate transient electric fields of intense ({approx}10{sup 17} W/cm{sup 2}) laser irradiated foils. We found out that these fields with an absolute peak of up to 10{sup 8} V/m extend over millimeter lateral extension and decay at nanosecond duration. Hence, they last much longer than the (approximately picosecond) laser excitation and extend much beyond the laser irradiation focus.

  1. Fuel ethanol from raw corn

    SciTech Connect

    Weller, C.L.; Rodda, E.D.; Steinberg, M.P.

    1983-06-01

    Crude amylase preparations were produced by growing Aspergillus awamori and A. niger on raw ground whole corn. These Koji preparations were used to hydrolyze the starch of raw ground whole corn to sugars during simultaneous fermentation of the sugars to ethanol by distillers active dry yeast. Ethanol concentrations of the fermentation beers were determined with gas-chromatography. These fermentations yielded an average of 89.6% theoretical ethanol compared to control, conventional, fermentations that had an average of 89.8%. Carbon dioxide evolutions were determined with use of Alwood valves. Both the Koji and conventional fermentations produced an average of 0.48 gram of carbon dioxide per gram of dry substrate starch within 72 hours. However, initially the conventional fermentation rate was greater. Koji dehydrated at 41/sup 0/C had no apparent detrimental effects on theoretical ethanol yield.

  2. Fuel ethanol from raw corn

    SciTech Connect

    Weller, C.L.; Rodda, E.D.; Steinberg, M.P.

    1983-06-01

    Crude amylase preparations were produced by growing Aspergillus awamori and A. niger on raw ground whole corn. These Koji preparations were used to hydrolyze the starch of raw ground whole corn to sugars during simultaneous fermentation of the sugars to ethanol by distillers active dry yeast. Ethanol concentrations of the fermentation beers were determined with gas-chromatography. These fermentations yielded an average of 89.6% theoretical ethanol compared to control, conventional, fermentations that had an average of 89.8%. Carbon dioxide evolutions were determined with use of Alwood valves. Both the Koji and conventional fermentations produced an average of 0.48 gram of carbon dioxide per gram of dry substrate starch within 72 hours. However, initially the conventional fermentation rate was greater. Koji dehydrated at 41 degrees C had no apparent detrimental effects on theoretical ethanol yield.

  3. Effect of transgenic corn hybrids and a soil insecticide on corn rootworm (Coleoptera: Chrysomelidae) beetle emergence in North Dakota

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Northern, Diabrotica barberi Smith & Lawrence, and western corn rootworms, D. virgifera virgifera LeConte, are economic pests of corn, Zea mays L. (Poaceae) in North Dakota. Many area corn growers rely on transgenic Bt (Bacillus thuringiensis) corn hybrids to manage corn rootworms. Our objective was...

  4. Wind Streaks

    NASA Technical Reports Server (NTRS)

    2004-01-01

    [figure removed for brevity, see original site]

    Released 12 July 2004 The atmosphere of Mars is a dynamic system. Water-ice clouds, fog, and hazes can make imaging the surface from space difficult. Dust storms can grow from local disturbances to global sizes, through which imaging is impossible. Seasonal temperature changes are the usual drivers in cloud and dust storm development and growth.

    Eons of atmospheric dust storm activity has left its mark on the surface of Mars. Dust carried aloft by the wind has settled out on every available surface; sand dunes have been created and moved by centuries of wind; and the effect of continual sand-blasting has modified many regions of Mars, creating yardangs and other unusual surface forms.

    Windstreaks are features caused by the interaction of wind and topographic landforms. The raised rims and bowls of impact craters causes a complex interaction such that the wind vortex in the lee of the crater can both scour away the surface dust and deposit it back in the center of the lee. If you look closely, you will see evidence of this in a darker 'rim' enclosing a brighter interior.

    Image information: VIS instrument. Latitude 6.9, Longitude 69.4 East (290.6 West). 19 meter/pixel resolution.

    Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time.

    NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in Pasadena.

  5. Production and analysis of citrinin in corn.

    PubMed Central

    Jackson, L K; Ciegler, A

    1978-01-01

    A convenient method for the production and analysis of citrinin in corn is described. Up to 2.964 g of citrinin can be produced by Penicillium citrinum per kg of corn by harvesting on day 21 or later. The analysis method has a lower detection limit of 0.25 ppm. Heating citrinin-contaminated corn destroys citrinin but may produce another toxin instead. PMID:727774

  6. Prevalence of a gene conferring sensitivity to nicosulfuron and mesotrione in sweet corn and field corn

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In previous research, a single gene in a herbicide-sensitive sweet corn inbred, Cr1, conditioned sensitivity to nicosulfuron, mesotrione and other postemergence herbicides. Many other sweet corn hybrids and inbreds and certain field corn inbreds also have been noted as being sensitive to certain po...

  7. The development of a "Green" aqueous enzymatic process to extract corn oil from corn germ

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Approximately 2.4 million tons of commercial corn oil were produced worldwide in 2012, compared to 2012 world production of palm oil (53.3 MT) and soybean oil (43.1 MT) according to FAS, USDA. Most commercial corn oil (~90%) is produced from corn germ that is expeller pressed and/or hexane extracte...

  8. Exploring Management Options for Increasing Corn Land on NY Farms Affected by Rising Corn Prices

    Technology Transfer Automated Retrieval System (TEKTRAN)

    New York dairy farms use their most fertile land to produce corn silage, an important component of their production system. Increasing demand for corn by ethanol producers is driving up corn grain prices. This is introducing a major shift into the NY dairy farm system by prompting farmers to place m...

  9. Potential Harvestable Corn Cob Biomass in Several Production Systems in the Western Corn Belt

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The proposed use of corn residues for biofuel production has increased interest in how much and what components of residue should/can be removed. One component of corn residue that is already being handled (corn cobs) might be an easily harvestable product that could be used for biofuel production. ...

  10. EFFECT OF SAMPLE PROCESSING PROCEDURES ON MEASUREMENT OF STARCH IN CORN SILAGE AND CORN GRAIN

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Methods for processing feedstuffs before analysis can affect analytical results. The effects of drying temperature and grinding method on starch analysis of corn silage and of grinding method on corn grain were evaluated. Corn silage samples dried at 55°C or 105°C and grain samples dried at 55°C w...

  11. Understanding successful resistance management: The European corn borer and Bt corn in the United States

    Technology Transfer Automated Retrieval System (TEKTRAN)

    European corn borer, Ostrinia nubilalis Hubner (Lepidoptera: Crambidae) has been a major pest of corn and other crops in North America since its accidental introduction nearly a hundred years ago. Wide adoption of transgenic corn that expresses toxins from Bacillus thuringiensis, referred to as Bt c...

  12. Proteins induced in corn (Zea mays) in response to the European corn borer (Ostrinia nubilalis)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The European corn borer (ECB, Ostrinia nubilalis) is a major pest of corn. ECB begin by feeding in the whorl tissue and then eventually tunnel into the stalk of the corn where they cause most of the damage. Tunneling can disrupt the transport of water and nutrients in the plant and it provides sites...

  13. Localized Search Cues in Corn Roots for Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Host-recognition cues that elicit a unique “tight-turning” behavior by larvae of the western corn rootworm, Diabrotica virgifera virgifera LeConte, were extracted from living corn roots with acetone. In behavioral bioassays, an acetone extract of corn roots had activity in the tight-turning bioassa...

  14. Evaluation of Elevated Dietary Corn Fiber from Corn Germ Meal in Growing Female Pigs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To evaluate the effects of high dietary corn fiber on growth and metabolic measures, female pigs (n= 48; initial body weight of 30.8 kg) were fed diets containing 0 to 38.6% solvent-extracted corn germ meal for 28 days. Increasing the level of dietary corn fiber had no impact on average daily gain o...

  15. Impact of corn variety on potential predators of larval corn rootworms (Coleoptera: Diabrotica spp.)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn rootworms (Coleoptera: Diabrotica spp.) are serious pests of maize around the world. Larvae feed on corn roots, which reduces uptake of water and nutrients and lowers plant yield. Current control practices rely on soil insecticides or genetically modified corn varieties. Although some researche...

  16. 3. Interior view of corn crib showing heavytimber framing, railed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Interior view of corn crib showing heavy-timber framing, railed trackway and corn car at upper level. - Laurel Valley Sugar Plantation, Corn Crib, 2 miles South of Thibodaux on State Route 308, Thibodaux, Lafourche Parish, LA

  17. Preliminary Performance Measurements for a Streak Camera with a Large-Format Direct-Coupled CCD Readout

    SciTech Connect

    Lerche, R A; McDonald, J W; Griffith, R L; de Dios, G V; Andrews, D S; Huey, A W; Bell, P M; Landen, O L; Jaanimagi, P A; Boni, R

    2004-04-13

    Livermore's ICF Program has a large inventory of optical streak cameras built in the 1970s and 1980s. The cameras are still very functional, but difficult to maintain because many of their parts are obsolete including the original streak tube and image-intensifier tube. The University of Rochester's Laboratory for Laser Energetics is leading an effort to develop a fully automated, large-format streak camera that incorporates modern technology. Preliminary characterization of a prototype camera shows spatial resolution better than 20 lp/mm, temporal resolution of 12 ps, line-spread function of 40 {micro}m (fwhm), contrast transfer ratio (CTR) of 60% at 10 lp/mm, and system sensitivity of 16 CCD electrons per photoelectron. A dynamic range of 60 for a 2 ns window is determined from system noise, linearity and sensitivity measurements.

  18. Frequency-Domain Streak Camera and Tomography for Ultrafast Imaging of Evolving and Channeled Plasma Accelerator Structures

    NASA Astrophysics Data System (ADS)

    Li, Zhengyan; Zgadzaj, Rafal; Wang, Xiaoming; Reed, Stephen; Dong, Peng; Downer, Michael C.

    2010-11-01

    We demonstrate a prototype Frequency Domain Streak Camera (FDSC) that can capture the picosecond time evolution of the plasma accelerator structure in a single shot. In our prototype Frequency-Domain Streak Camera, a probe pulse propagates obliquely to a sub-picosecond pump pulse that creates an evolving nonlinear index "bubble" in fused silica glass, supplementing a conventional Frequency Domain Holographic (FDH) probe-reference pair that co-propagates with the "bubble". Frequency Domain Tomography (FDT) generalizes Frequency-Domain Streak Camera by probing the "bubble" from multiple angles and reconstructing its morphology and evolution using algorithms similar to those used in medical CAT scans. Multiplexing methods (Temporal Multiplexing and Angular Multiplexing) improve data storage and processing capability, demonstrating a compact Frequency Domain Tomography system with a single spectrometer.

  19. Picosecond-resolved X-ray absorption spectroscopy at low signal contrast using a hard X-ray streak camera

    SciTech Connect

    Adams, Bernhard W.; Rose-Petruck, Christoph; Jiao, Yishuo

    2015-06-24

    A picosecond-resolving hard-X-ray streak camera has been in operation for several years at Sector 7 of the Advanced Photon Source (APS). Several upgrades have been implemented over the past few years to optimize integration into the beamline, reduce the timing jitter, and improve the signal-to-noise ratio. These include the development of X-ray optics for focusing the X-rays into the sample and the entrance slit of the streak camera, and measures to minimize the amount of laser light needed to generate the deflection-voltage ramp. For the latter, the photoconductive switch generating the deflection ramp was replaced with microwave power electronics. With these, the streak camera operates routinely at 88 MHz repetition rate, thus making it compatible with all of the APS fill patterns including use of all the X-rays in the 324-bunch mode. Sample data are shown to demonstrate the performance.

  20. Slit-mounted LED fiducial system for rotating mirror streak cameras

    SciTech Connect

    Shaw, L.L.; Muelder, S.A.; Rivera, A.T.

    1991-01-01

    We have developed a fiducial system for rotating mirror streak cameras that utilizes light emitting diodes mounted at the slit position of the camera. The diodes are driven to the required high brightness by a unique pulse power circuit designed to provide high voltage, high current pulses 18 nanoseconds in length at a frequency of up to 2.5 megahertz. The availability of super bright light emitting diodes with a wavelength of 630 to 640 nanometers allows us to record fiducial pulses, at streaking speeds in excess of 20mm per microsecond, on all the black and white films commonly used in high speed photography. The time marks on the film record are referenced to the real time of the experiment from a clock-driver that controls the start and frequency of the fiducial pulse train and by three adjustable and discreet blanked fiducials. This paper discusses the development of this system and describes the full setup as used at LLNL. 6 refs., 4 figs.

  1. Synchroscan streak camera imaging at a 15-MeV photoinjector with emittance exchange

    NASA Astrophysics Data System (ADS)

    Lumpkin, A. H.; Ruan, J.; Thurman-Keup, R.

    2012-09-01

    At the Fermilab A0 photoinjector facility, bunch-length measurements of the laser micropulse and the e-beam micropulse have been done in the past with a fast single-sweep module of the Hamamatsu C5680 streak camera with an intrinsic shot-to-shot trigger jitter of 10-20 ps. We have upgraded the camera system with the synchroscan module tuned to 81.25 MHz to provide synchronous summing capability with less than 1.5 ps FWHM trigger jitter and a phase-locked delay box to provide phase stability of ˜1 ps over 10 s of minutes. These steps allowed us to measure both the UV laser pulse train at 263 nm and the e-beam via optical transition radiation (OTR). Due to the low electron beam energies and OTR signals, we typically summed over 50 micropulses with 0.25-1 nC per micropulse. The phase-locked delay box allowed us to assess chromatic temporal effects and instigated another upgrade to an all-mirror input optics barrel. In addition, we added a slow sweep horizontal deflection plug-in unit to provide dual-sweep capability for the streak camera. We report on a series of measurements made during the commissioning of these upgrades including bunch-length and phase effects using the emittance exchange beamline and simultaneous imaging of a UV drive laser component, OTR, and the 800 nm diagnostics laser.

  2. An Optical Streak Diagnostic for Observing Anode-Cathode Plasmas for Radiographic Source Development

    SciTech Connect

    Droemer, Darryl W.; Crain, Marlon D.; Lare, Gregory A.; Bennett, Nichelle L.; Johnston, Mark D.

    2013-06-13

    National Security Technologies, LLC, and Sandia National Laboratories are collaborating in the development of pulsed power–driven flash x-ray radiographic sources that utilize high-intensity electron beam diodes. The RITS 6 (Radiographic Integrated Test Stand) accelerator at Sandia is used to drive a self magnetic pinch diode to produce a Bremsstrahlung x-ray source. The high electric fields and current densities associated with these short A-K gap pinch beam diodes present many challenges in diode development. Plasmas generated at both the anode and cathode affect the diode performance, which is manifested in varying spot (source) sizes, total dose output, and impedance profiles. Understanding the nature of these plasmas including closure rates and densities is important in modeling their behavior and providing insight into their mitigation. In this paper we describe a streak camera–based optical diagnostic that is capable of observing and measuring plasma evolution within the A-K gap. By imaging a region of interest onto the input slit of a streak camera, we are able to produce a time-resolved one-dimensional image of the evolving plasma. Typical data are presented.

  3. Nonlinear response of the photocathode of an x-ray streak camera to UV light

    SciTech Connect

    Kyrala, G.A.; Oro, D.M.; Studebaker, J.K.; Wood, W.M.; Schappert, G.T.; Watts, S.; Fulton, R.D.

    1994-09-01

    We have found that a potassium-iodide photocathode of an x-ray streak camera responds to UV light at {lambda}=308 nm. The photocathode surface work function, 6.5 eV, is larger than the 4 eV energy of the UV photon, hence the source of the response is interesting. We will present results on the response of a transmission type potassium-iodide photocathode to the UV light from a {lambda}308 nm, subpicosecond XeCl laser and from a {lambda}=326 nm HeCd laser. We will test for the nonlinearity of the yield to measure of the number of photons that are needed to be absorbed before a signal is recorded. We will present data on the effect of the UV irradiance on the yield, as well as on the temporal width of the recorded signal. We will give an explanation of the observation and its effect on the dynamic-range response of the streak-camera. We will show that the response is linear with the incident irradiance, up to an incident irradiance of 10{sup 8} W/cm{sup 2} and we will explain the observation.

  4. Automatic Reacquisition of Satellite Positions by Detecting Their Expected Streaks in Astronomical Images

    NASA Astrophysics Data System (ADS)

    Levesque, M.

    Artificial satellites, and particularly space junk, drift continuously from their known orbits. In the surveillance-of-space context, they must be observed frequently to ensure that the corresponding orbital parameter database entries are up-to-date. Autonomous ground-based optical systems are periodically tasked to observe these objects, calculate the difference between their predicted and real positions and update object orbital parameters. The real satellite positions are provided by the detection of the satellite streaks in the astronomical images specifically acquired for this purpose. This paper presents the image processing techniques used to detect and extract the satellite positions. The methodology includes several processing steps including: image background estimation and removal, star detection and removal, an iterative matched filter for streak detection, and finally false alarm rejection algorithms. This detection methodology is able to detect very faint objects. Simulated data were used to evaluate the methodology's performance and determine the sensitivity limits where the algorithm can perform detection without false alarm, which is essential to avoid corruption of the orbital parameter database.

  5. PS-1/S1 picosecond streak camera application for multichannel laser system diagnostics

    SciTech Connect

    Garanin, S G; Bel'kov, S A; Rogozhnikov, G S; Rukavishnikov, N N; Romanov, V V; Voronich, I N; Vorob'ev, N S; Gornostaev, P B; Lozovoi, V I; Shchelev, M Ya

    2014-08-31

    A PS-1/S1 picosecond image-tube streak camera (ITSC) with slit scan (streak camera), developed and manufactured at the General Physics Institute RAS, has been used to measure the spatiotemporal characteristics of ultrashort laser pulses generated by a petawatt-power laser installation 'FEMTO' at the Institute of Laser Physics Research in Sarov. It is found that such a camera is suitable for measuring the spatial and temporal parameters of single laser pulses with an accuracy of about one picosecond. It is shown that the intensity time profile of a train of picosecond pulses may be precisely defined for the pulses separated in time by a few picoseconds. The camera allows the contrast of radiation to be determined with a high (no less than 10{sup 3}) accuracy; spatial distribution of the laser pulses can be measured with an accuracy of tens of microns, and the temporal separation of single laser pulses can be identified with an accuracy of 1 – 1.5 ps. (extreme light fields and their applications)

  6. Attosecond photoelectron streaking with enhanced energy resolution for small-bandgap materials.

    PubMed

    Guggenmos, Alexander; Akil, Ayman; Ossiander, Marcus; Schäffer, Martin; Azzeer, Abdallah Mohammed; Boehm, Gerhard; Amann, Markus-Christian; Kienberger, Reinhard; Schultze, Martin; Kleineberg, Ulf

    2016-08-15

    Attosecond photoelectron streaking spectroscopy allows time-resolved electron dynamics with a temporal resolution approaching the atomic unit of time. Studies have been performed in numerous systems, including atoms, molecules, and surfaces, and the quest for ever higher temporal resolution called for ever wider spectral extent of the attosecond pulses. For typical experiments relying on attosecond pulses with a duration of 200 as, the time-bandwidth limitation for a Gaussian pulse implies a minimal spectral bandwidth larger than 9 eV translating to a corresponding spread of the detected photoelectron kinetic energies. Here, by utilizing a specially tailored narrowband reflective XUV multilayer mirror, we explore experimentally the minimal spectral width compatible with attosecond time-resolved photoelectron spectroscopy while obtaining the highest possible spectral resolution. The validity of the concept is proven by recording attosecond electron streaking traces from the direct semiconductor gallium arsenide (GaAs), with a nominal bandgap of 1.42 eV at room temperature, proving the potential of the approach for tracking charge dynamics also in these technologically highly relevant materials that previously have been inaccessible to attosecond science. PMID:27519070

  7. Attosecond time-resolved streaked photoemission from Mg-covered W(110) surfaces

    NASA Astrophysics Data System (ADS)

    Liao, Qing; Thumm, Uwe

    2015-09-01

    We formulate a quantum-mechanical model for infrared-streaked photoelectron (PE) emission by ultrashort extreme ultraviolet (XUV) pulses from an adsorbate-covered metal surface, exposing the influence of microscopic PE dispersion in substrate and adsorbate on the interpretation of streaked photoemission spectra and photoemission time delays. We validate this numerical model first by reproducing measured relative photoemission delays (a) between valence-band and 2 p -core-level (CL) PEs emitted from clean Mg(0001) surfaces and (b) between conduction-band (CB) and 4 f -CL PEs from clean W(110) surfaces at two XUV-pulse central photon energies. Next, applying this model to ultrathin Mg adsorbate layers on W(110) substrates, we reproduce (i) the measured nonmonotonic dependence of relative photoemission delays between CB and Mg (2 p ) PEs and (ii) the monotonic dependence of relative delays between W (4 f ) and Mg (2 p ) PEs in a recent experiment [S. Neppl et al., Nature (London) 517, 342 (2015), 10.1038/nature14094].

  8. Single-shot visualization of evolving laser wakefields using an all-optical streak camera

    NASA Astrophysics Data System (ADS)

    Li, Zhengyan; Tsai, Hai-En; Zhang, Xi; Pai, Chih-Hao; Chang, Yen-Yu; Zgadzaj, Rafal; Wang, Xiaoming; Khudik, Vladimir; Shvets, Gennady; Downer, Michael

    2014-10-01

    We visualize ps-time-scale evolution of an electron density bubble, a wake structure created in atmospheric density plasma by an intense ultrashort laser pulse, from the phase ``streak'' that the bubble imprints onto a probe pulse that crosses its path obliquely. Phase streaks, recovered in one shot using frequency-domain interferometry, reveal formation, propagation and coalescence of the bubble within a 3 mm long ionized helium gas target. 3D particle-in-cell (PIC) simulations validate the observed density-dependent bubble evolution, and correlate it with generation of a quasi-monoenergetic ~100 MeV electron beam. The results provide a basis for understanding optimized electron acceleration at plasma density ne ~2e19 cm-3, at which the bubble formed and persisted until the jet exit, enabling acceleration over a distance slightly exceeding the dephasing length. In contrast, at lower density, electrons accelerated inefficiently due to weak laser self-focusing and late bubble formation. At higher density, overly strong self-focusing also led to low quality electrons due to early bubble formation and strong dephasing. Bubble coalescence due to beam loading further degraded electron acceleration. This work was supported by DOE grants DESC0011617 and DE-SC0007889 and by the NSF-DOE Partnership in Plasma Science program.

  9. A Method for Analysing High Resolution, Time Domain, Streak Camera Calibration Data

    SciTech Connect

    Silbernagel, C

    2004-07-02

    Many experiments that require a highly accurate continuous time history of photon emission incorporate streak cameras into their setup. Nonlinear recordings both in time and spatial displacement are inherent to streak camera measurements. These nonlinearities can be attributed to sweep rate electronics, curvature of the electron optics, the magnification, and resolution of the electron optics. These nonlinearities are systematic; it has been shown that a short pulse laser source, an air-spaced etalon of known separation, and a defined spatial resolution mask can provide the proper image information to correct for the resulting distortion. A set of Interactive Data Language (IDL){sup 1} software routines were developed to take a series of calibration images showing temporally and spatially displaced points, and map these points from a nonlinear to a linear space-time resultant function. This correction function, in combination with standardized image correction techniques, can be applied to experiment data to minimize systematic errors and improve temporal and spatial resolution measurements.

  10. PS-1/S1 picosecond streak camera application for multichannel laser system diagnostics

    NASA Astrophysics Data System (ADS)

    Garanin, S. G.; Bel'kov, S. A.; Rogozhnikov, G. S.; Rukavishnikov, N. N.; Romanov, V. V.; Voronich, I. N.; Vorob'ev, N. S.; Gornostaev, P. B.; Lozovoi, V. I.; Shchelev, M. Ya

    2014-08-01

    A PS-1/S1 picosecond image-tube streak camera (ITSC) with slit scan (streak camera), developed and manufactured at the General Physics Institute RAS, has been used to measure the spatiotemporal characteristics of ultrashort laser pulses generated by a petawatt-power laser installation 'FEMTO' at the Institute of Laser Physics Research in Sarov. It is found that such a camera is suitable for measuring the spatial and temporal parameters of single laser pulses with an accuracy of about one picosecond. It is shown that the intensity time profile of a train of picosecond pulses may be precisely defined for the pulses separated in time by a few picoseconds. The camera allows the contrast of radiation to be determined with a high (no less than 103) accuracy; spatial distribution of the laser pulses can be measured with an accuracy of tens of microns, and the temporal separation of single laser pulses can be identified with an accuracy of 1 - 1.5 ps.

  11. Gas-phase lifetimes of nucleobase analogues by picosecond pumpionization and streak techniques.

    PubMed

    Blaser, Susan; Frey, Hans-Martin; Heid, Cornelia G; Leutwyler, Samuel

    2014-01-01

    The picosecond (ps) timescale is relevant for the investigation of many molecular dynamical processes such as fluorescence, nonradiative relaxation, intramolecular vibrational relaxation, molecular rotation and intermolecular energy transfer, to name a few. While investigations of ultrafast (femtosecond) processes of biological molecules, e.g. nucleobases and their analogues in the gas phase are available, there are few investigations on the ps time scale. We have constructed a ps pump-ionization setup and a ps streak camera fluorescence apparatus for the determination of lifetimes of supersonic jet-cooled and isolated molecules and clusters. The ps pump-ionization setup was used to determine the lifetimes of the nucleobase analogue 2-aminopurine (2AP) and of two 2AP˙(H2O)n water cluster isomers with n=1 and 2. Their lifetimes lie between 150 ps and 3 ns and are strongly cluster-size dependent. The ps streak camera setup was used to determine accurate fluorescence lifetimes of the uracil analogue 2-pyridone (2PY), its self-dimer (2PY)2, two isomers of its trimer (2PY)3 and its tetramer (2PY)4, which lie in the 7-12 ns range. PMID:24983611

  12. The dynamic nature and spectral characteristics of low-albedo slope streaks on Mars and their possible hydrologic implications

    NASA Astrophysics Data System (ADS)

    Mushkin, A.; Stillman, D. E.; Gillespie, A. R.; Montgomery, D. R.; Schreiber, B. C.; Hibbitts, C.

    2014-12-01

    Low-albedo down-slope streaks that form repeatedly within weekly time-scales and subsequently fade over seasonal to decadal periods are commonly observed in the tropical and mid-latitudes of Mars. 'Dry' mass-wasting processes vs. 'wet' modification of the surface by aqueous phases are the mechanisms typically considered to explain their formation. Recently, high frequency HiRISE image time-series of seasonal recurrence, incremental growth and fading of small (meter-decameter scale) slope streaks, also termed 'recurring slope lineae' (RSL), have been presented in support of a 'wet' origin likely associated with brine seepage. Here, we present new results that demonstrate comparable recurrence, incremental growth and fading characteristics over yearly time-scales for decameter-kilometer scale low-albedo slope-streaks in Lycus Sulci, Amazonis Planitia and Arabia Terra. These dynamic characteristics support the previous association of low-albedo slope streaks with brine seepage based on their geomorphic and spectral relations with surrounding unaffected slopes. Low-albedo slope streaks are typically not associated with detectable erosion or terminal, down-slope depositional activity at the resolution of 25 cm/pixel HiRISE images. CRISM observations consistently indicate that darkened slope-streak surfaces are spectrally enriched in FeOx and are void of detectable water/ice spectral absorption bands. Thus, the liquid seeps considered for the formation of meter to kilometer scale slope streaks are likely low-volume transient events that evaporate and/or freeze and sublime leaving behind a meta-stable dry precipitate that 'stains' the surface dark and may provide insights into the possible composition of such brines. Slope streak formation through a 'wet' brine seepage mechanism supports the possible presence of pressurized sub-surface aquifers that may be released via faults or cracks able to produce recurring transient discharge events during favorably warm daily

  13. Viruses in maize and johnsongrass in southern ohio.

    PubMed

    Stewart, L R; Teplier, R; Todd, J C; Jones, M W; Cassone, B J; Wijeratne, S; Wijeratne, A; Redinbaugh, M G

    2014-12-01

    ABSTRACT The two major U.S. maize viruses, Maize dwarf mosaic virus (MDMV) and Maize chlorotic dwarf virus (MCDV), emerged in southern Ohio and surrounding regions in the 1960s and caused significant losses. Planting resistant varieties and changing cultural practices has dramatically reduced virus impact in subsequent decades. Current information on the distribution, diversity, and impact of known and potential U.S. maize disease-causing viruses is lacking. To assess the current reservoir of viruses present at the sites of past disease emergence, we used a combination of serological testing and next-generation RNA sequencing approaches. Here, we report enzyme-linked immunosorbent assay and RNA-Seq data from samples collected over 2 years to assess the presence of viruses in cultivated maize and an important weedy reservoir, Johnsongrass (Sorghum halepense). Results revealed a persistent reservoir of MDMV and two strains of MCDV in Ohio Johnsongrass. We identified sequences of several other grass-infecting viruses and confirmed the presence of Wheat mosaic virus in Ohio maize. Together, these results provide important data for managing virus disease in field corn and sweet corn maize crops, and identifying potential future virus threats. PMID:24918609

  14. Corn silage from corn treated with foliar fungicide and performance of Holstein cows.

    PubMed

    Haerr, K J; Lopes, N M; Pereira, M N; Fellows, G M; Cardoso, F C

    2015-12-01

    Foliar fungicide application to corn plants is used in corn aimed for corn silage in the dairy industry, but questions regarding frequency of application and its effect on corn silage quality and feed conversion when fed to dairy cows remain prevalent. The objective of this study was to evaluate the effects of various foliar fungicide applications to corn on dry matter intake (DMI), milk production, and milk composition when fed to dairy cows. Sixty-four Holstein cows with parity 2.5±1.5, 653±80kg of body weight, and 161±51d in milk were blocked and randomly assigned to 1 of 4 corn silage treatments (total mixed ration with 35% of the dry matter as corn silage). Treatments were as follows: control (CON), corn silage with no applications of foliar fungicide; treatment 1 (1X), corn silage from corn that received 1 application of pyraclostrobin (PYR) foliar fungicide (Headline; BASF Corp.) at corn vegetative stage 5; treatment 2 (2X), corn silage from corn that received the same application as 1X plus another application of a mixture of PYR and metconazole (Headline AMP; BASF Corp.) at corn reproductive stage 1 ("silking"); and treatment 3 (3X), corn silage from corn that received the same applications as 2X as well as a third application of PYR and metconazole at reproductive stage 3 ("milky kernel"). Corn was harvested at about 32% dry matter and 3/4 milk line stage of kernel development and ensiled for 200d. Treatments were fed to cows for 5wk, with the last week being used for statistical inferences. Week -1 was used as a covariate in the statistical analysis. Dry matter intake tended to be lower for cows fed corn silage treated with fungicide than CON (23.8, 23.0, 19.5, and 21.3kg for CON, 1X, 2X, and 3X, respectively). A linear treatment effect for DMI was observed, with DMI decreasing as foliar fungicide applications increased. Treatments CON, 1X, 2X, and 3X did not differ for milk yield (34.5, 34.5, 34.2, and 34.4kg/d, respectively); however, a trend for

  15. Responses of Maize (Zea mays L.) near isogenic lines carrying Wsm1, Wsm2 and Wsm3 to three viruses in the Potyviridae

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three independent genes controlling resistance to Wheat streak mosaic virus (WSMV; family Potyviridae; genus Tritimovirus) were previously identified on chromosomes six (Wsm1), three (Wsm2) and ten (Wsm3) in the resistant maize (Zea mays L.) inbred line Pa405. Near isogenic lines (NIL) carrying eac...

  16. Behavioral responses of western corn rootworm larvae to volatile semiochemicals from corn seedlings.

    PubMed

    Hibbard, B E; Bjostad, L B

    1988-06-01

    Corn seedling volatiles collected cryogenically are highly attractive to western corn rootworm larvae,Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), in a laboratory bioassay. Carbon dioxide is known as an attractant for western corn rootworm larvae, and the amount of carbon dioxide in the cryogenic collections was measured with an infrared gas analyzer. In a choice test between a source containing carbon dioxide alone and a source containing corn seedling volatiles with an equal amount of carbon dioxide (verified by infrared gas analysis), western corn rootworm larvae chose the corn volatile source significantly more often than the side with carbon dioxide alone. This indicates that carbon dioxide is only one of the volatiles from corn seedlings that is behaviorally important and that other compounds of behavioral importance are present as well. PMID:24276403

  17. Starlink corn: a risk analysis.

    PubMed Central

    Bucchini, Luca; Goldman, Lynn R

    2002-01-01

    Modern biotechnology has dramatically increased our ability to alter the agronomic traits of plants. Among the novel traits that biotechnology has made available, an important group includes Bacillus thuringiensis-derived insect resistance. This technology has been applied to potatoes, cotton, and corn. Benefits of Bt crops, and biotechnology generally, can be realized only if risks are assessed and managed properly. The case of Starlink corn, a plant modified with a gene that encodes the Bt protein Cry9c, was a severe test of U.S. regulatory agencies. The U.S. Environmental Protection Agency had restricted its use to animal feed due to concern about the potential for allergenicity. However, Starlink corn was later found throughout the human food supply, resulting in food recalls by the Food and Drug Administration and significant disruption of the food supply. Here we examine the regulatory history of Starlink, the assessment framework employed by the U.S. government, assumptions and information gaps, and the key elements of government efforts to manage the product. We explore the impacts on regulations, science, and society and conclude that only significant advances in our understanding of food allergies and improvements in monitoring and enforcement will avoid similar events in the future. Specifically, we need to develop a stronger fundamental basis for predicting allergic sensitization and reactions if novel proteins are to be introduced in this fashion. Mechanisms are needed to assure that worker and community aeroallergen risks are considered. Requirements are needed for the development of valid assays so that enforcement and post market surveillance activities can be conducted. PMID:11781159

  18. Integrated Corn-Based Bio-Refinery

    SciTech Connect

    2006-04-01

    The Integrated Corn-Based Bio-Refinery (ICBR) process will use new technology to convert corn grain and stover into fermentable sugars for the parallel production of value-added chemicals such as 1,3-propanediol (PDO) and fuel ethanol.

  19. Climate forecasts for corn producer decision making

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn is the most widely grown crop in the Americas, with annual production in the United States of approximately 332 million metric tons. Improved climate forecasts, together with climate-related decision tools for corn producers based on these improved forecasts, could substantially reduce uncertai...

  20. Corn fiber utilization for production of Schizophyllan

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn fiber is an abundant coproduct of the corn wet milling process, primarily composed of the seed pericarp and adherent starch. Schizophyllan is a biopolymer composed entirely of glucose, with a ß-1,3-linked backbone and single ß-1,6-linked glucose side chains at every third residue, produced by t...

  1. INTACT OR UNIT-KERNEL SWEET CORN

    EPA Science Inventory

    This report evaluates process and product modifications in canned and frozen sweet corn manufacture with the objective of reducing the total effluent produced in processing. In particular it evaluates the proposed replacement of process steps that yield cut or whole kernel corn w...

  2. The corn blight problem: 1970 and 1971

    NASA Technical Reports Server (NTRS)

    Bauer, M. E.

    1972-01-01

    Southern corn leaf blight is caused by the fungus, Helminthosporium maydis. Race T of H maydis adapted itself to the Texas male sterile cytoplasm corn. The problems caused by this variety of the blight in 1970 and 1971 are discussed, as well as the symptoms and development of the disease.

  3. Critical Precipitation Period for Dryland Corn Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Grain yields for dryland corn (Zea mays L.) production in the semi-arid Great Plains of the United States can be very unpredictable because of the erratic nature of growing season precipitation. Because inputs costs for corn production can be very high, farmers need to have a tool that will help the...

  4. Corn production with Spray, LEPA, and SDI

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corn, a major irrigated crop in the U.S. Great Plains, has a large irrigation requirement making efficient, effective irrigation technology important. The objective of this paper was to compare corn productivity for different irrigation methods and irrigation rates in 2009 and 2010 at Bushland, Texa...

  5. Active sensor assessment of corn nitrogen status

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Use of active sensor measurements of in-season corn (Zea mays L.) nitrogen (N) status for directing spatially-variable N applications has been advocated to improve N use efficiency. However, first it is necessary to confirm that active sensors can reliably assess corn N status. Our research goals we...

  6. CONVERSION OF CORN FIBROUS MATERIAL INTO ETHANOL

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Over 1.7 billion gallons of fuel ethanol were produced in the U.S. (2001), over 90% of which was produced from corn. Corn is prepared for ethanol fermentation by either wet milling or dry grinding in approximately equal volumes. In both processes, the fibrous components of the kernel are folded in...

  7. Erraticity of sprinkler irrigated corn in 2011

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sprinkler non-uniformity effects on corn are accentuated in a drought. The lack of rainfall during the 2011 corn growing season in Colby, Kansas illustrated many fields irrigated by center pivot sprinklers that had poor crops due to non-uniform irrigation. Many of these effects were due to use of ...

  8. Analysis of Dark Slope Streaks on Mars based on Multitemporal HRSC Data

    NASA Astrophysics Data System (ADS)

    Schreiner, Björn; van Gasselt, Stephan; Jan-Peter, Muller

    2016-04-01

    Recurring slope lineae (RSL) on Mars are dark and narrow downhill oriented surface features found in equatorial regions (1) associated with water or hydrated salt flows (2). On the other hand there are Dark Slope Streaks which seem to be dry avalanches on dust covered slopes (3). The origin of both ist still under discussion. We found linear features in eastern Noctis Labyrinthus region (6°S, 265°E) with lengths of up to several kilometres and lateral extensions of 20-30 metres. As described by (4), RSL fade and recur in the same location over multiple Mars years. Similarily, Dark Slope Streaks form on at least annual to decade-long timescales (5). During 10 years of HRSC observation time (2005-2015) several linear features in Noctis Labyrinthus changed in visibility. Slope parameters and seasonal illumination conditions are investigated based on a digital elevation model derived from HRSC data. For large datasets a feature identification is presented which involves spatial filtering in conjunction with elevation data analysis. The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under iMars grant agreement n° 607379. (1) McEwen, A.S., et al. (2014): Recurring slope lineae in equatorial regions of Mars. Nat. Geosci 7: 53-58. (2) Ojha, L. et al. (2015): Spectral evidence for hydrated salts in recurring slope linear on Mars. Nat. Geosci, DOI:10.1038/NGEO2546. (3) Sullivan, R. et al. (2001). Mass Movement Slope Streaks Imaged by the Mars Orbiter Camera. J. Geophys. Res., 106(E10), 23,607-23,633. (4) McEwen, A.S., et al. (2011): Seasonal Flows on Warm Martian Slopes. Science, Vol. 333, Issue 6043, pp. 740-743. (5) Malin, M.C.; Edgett, K.S. (2001). Mars Global Surveyor Mars Orbiter Camera: Interplanetary cruise through primary mission. J. Geophys. Res., 106(E10), 23,429-23,570.

  9. GPU-accelerated Faint Streak Detection for Uncued Surveillance of LEO

    NASA Astrophysics Data System (ADS)

    Zimmer, P.; Ackermann, M.; McGraw, J. T.

    2013-09-01

    By astronomical standards, small objects (<10cm) in LEO illuminated by the Sun under terminator conditions are quite bright, depositing 100's to 1000's of photons per second into small telescope apertures (< 1m diameter). The challenge in discovering these objects with no a priori knowledge of their orbit (i.e. uncued surveillance) is that their relative motion with respect to a ground-based telescope makes them appear to have large angular rates of motion, up to and exceeding 1 degree per second. Thus in even a short exposure, the signal from the object is smeared out in a streak with low signal-to-noise per pixel. Go Green Termite (GGT), Inc. of Gilroy, CA, in collaboration with the University of New Mexico (UNM), is building two proof-of-concept wide-field imaging systems to test, develop and prove a novel streak detection technique. The imaging systems are built from off-the-shelf optics and detectors resulting in a 350mm aperture and a 6 square degree field of view. For streak detection, field of view is of critical importance because the maximum exposure time on the object is limited by its crossing time. In this way, wider fields of view impact surveys for LEO objects both by increasing the survey volume and increasing sensitivity. Using our newly GPU-accelerated detection scheme, the proof-of-concept systems are expected to be able to detect objects fainter than 12th magnitude moving at 1 degree per second and possibly as faint as 13th magnitude for slower moving objects. Meter-class optical systems using these techniques should be able to detect objects fainter than 14th magnitude, which is roughly equivalent to a golf ball at 1000km altitude. The goal of this work is to demonstrate a scalable system for near real time detection of fast moving objects that can be then handed off to other instruments capable of tracking and characterizing them. The two proof-of-concept systems, separated by ~30km, work together by taking simultaneous images of the same

  10. Corn plant locating by image processing

    NASA Astrophysics Data System (ADS)

    Jia, Jiancheng; Krutz, Gary W.; Gibson, Harry W.

    1991-02-01

    The feasibility investigation of using machine vision technology to locate corn plants is an important issue for field production automation in the agricultural industry. This paper presents an approach which was developed to locate the center of a corn plant using image processing techniques. Corn plants were first identified using a main vein detection algorithm by detecting a local feature of corn leaves leaf main veins based on the spectral difference between mains and leaves then the center of the plant could be located using a center locating algorithm by tracing and extending each detected vein line and evaluating the center of the plant from intersection points of those lines. The experimental results show the usefulness of the algorithm for machine vision applications related to corn plant identification. Such a technique can be used for pre. cisc spraying of pesticides or biotech chemicals. 1.

  11. Wheat streak mosaic: A classic case of plant disease impact on soil water content and crop water-use efficiency

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this article, we describe the relationship between wheat streak mosaic (WSM) severity and soil water content as a prime example of the effect of a plant disease on soil water status and its implications for irrigated agriculture. The present study was part of a larger investigation which included...

  12. Environmental factors influencing the development of black leaf streak (Mycosphaerella fijiensis Morelet) on bananas in Puerto Rico.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effects of environmental factors on the development of black leaf streak (BLS) were studied in Puerto Rico under field conditions. Environmental factors evaluated included temperature, relative humidity, rainfall and solar radiation. Their effect on BLS was determined by recording the youngest...

  13. Evaluation of triticale accessions for resistance to wheat bacterial leaf streak caused by Xanthomonas translucens pv. undulosa

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The bacterium Xanthomonas translucens pv. undulosa (Xtu) causes bacterial leaf streak (BLS) on wheat and other small grains. Several triticale accessions were reported to possess high levels of resistance to wheat Xtu strains. In this study, we evaluated a worldwide collection of 502 triticale acces...

  14. Particle streak velocimetry-optical coherence tomography: a novel method for multidimensional imaging of microscale fluid flows

    PubMed Central

    Zhou, Kevin C.; Huang, Brendan K.; Gamm, Ute A.; Bhandari, Vineet; Khokha, Mustafa K.; Choma, Michael A.

    2016-01-01

    We present a new OCT method for flow speed quantification and directional velocimetry: particle streak velocimetry-OCT (PSV-OCT). PSV-OCT generates two-dimensional, 2.5-vector component (vx,|vy|,vz) maps of microscale flow velocity fields. Knowledge of 2.5-vector components also enables the estimation of total flow speed. The enabling insight behind PSV-OCT is that tracer particles in sparsely-seeded fluid flow trace out streaks in (x,z,t)-space. The streak orientations in x-t and z-t yield vx and vz, respectively. The in-plane (x-z plane) residence time yields the out-of-plane speed |vy|. Vector component values are generated by fitting streaks to a model of image formation that incorporates equations of motion in 3D space. We demonstrate cross-sectional estimation of (vx,|vy|,vz) in two important animal models in ciliary biology: Xenopus embryos (tadpoles) and mouse trachea. PMID:27375926

  15. Modified ecometric technique (four-quadrant sequential streak) to evaluate Campylobacter enrichment broth proficiency in suppressing background microflora

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ecometric technique is a semi-quantitative scoring method used for quality control of culture media in microbiological laboratories. The technique involves inoculation with defined populations of specific culture onto solid media via a standardized chronological streaking technique, leading to ever-...

  16. First report of bacterial streak of fennel (Foeniculum vulgare) in California caused by Pseudomonas syringae pv. apii

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A new bacterial streak disease appeared on fennel leaves, stems and bulbs grown in Salinas California production fields. Initial symptoms consisted of small black lesions on stems that spread down the stem to the bulbs and up the stem to leaves as the disease progressed. The disease rendered the pl...

  17. Wheat Streak Mosaic: A classic case of plant disease impact on soil water content and crop water-use efficiency

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this article, we describe the relationship between wheat streak mosaic (WSM) severity and soil water content as a prime example of the effect of a plant disease on soil water status and its implications for irrigated agriculture. The present study was part of a larger investigation which included...

  18. Resolution limitations and optimization of the ITT F4157 streak tube focus for fast (10 ps) operation

    SciTech Connect

    Lerche, R.A.; Grasz, E.L.; Griffith, R.L.; Simpson, R.A.; Posey, R.

    1987-11-01

    The ITT F4157 image tube is biased at voltages far from the original design for operation in an ultrafast (10 ps) streak camera. Its output resolution at streak camera operating potentials has been measured as a function of input slit width, incident-light wavelength, and focus-grid voltage. The results are similar to those reported for the RCA C73435 streak tube. Indeed, the two tubes can be substituted for each other with minor mechanical modifications. The temporal resolution is insensitive to focus-grid voltage for a narrow (50 ..mu..m) input slit, but is very sensitive to focus-grid voltage for a wide (500 ..mu..m) input slit. Spatial resolution is nearly independent of focus-grid voltage for values that give good temporal resolution. Both temporal and spatial resolution depend on the incident-light wavelength. Streak camera operation is simulated with a computer program that calculates photoelectron trajectories. Electron ray tracing describes the observed effects of slit width, incident-light wavelength, and focus-grid voltage on the output resolution. 6 refs., 10 figs., 1 tab.

  19. Particle streak velocimetry-optical coherence tomography: a novel method for multidimensional imaging of microscale fluid flows.

    PubMed

    Zhou, Kevin C; Huang, Brendan K; Gamm, Ute A; Bhandari, Vineet; Khokha, Mustafa K; Choma, Michael A

    2016-04-01

    We present a new OCT method for flow speed quantification and directional velocimetry: particle streak velocimetry-OCT (PSV-OCT). PSV-OCT generates two-dimensional, 2.5-vector component (vx ,|vy |,vz ) maps of microscale flow velocity fields. Knowledge of 2.5-vector components also enables the estimation of total flow speed. The enabling insight behind PSV-OCT is that tracer particles in sparsely-seeded fluid flow trace out streaks in (x,z,t)-space. The streak orientations in x-t and z-t yield vx and vz , respectively. The in-plane (x-z plane) residence time yields the out-of-plane speed |vy |. Vector component values are generated by fitting streaks to a model of image formation that incorporates equations of motion in 3D space. We demonstrate cross-sectional estimation of (vx ,|vy |,vz ) in two important animal models in ciliary biology: Xenopus embryos (tadpoles) and mouse trachea. PMID:27375926

  20. Association mapping of quantitative trait loci responsible for resistance to Bacterial Leaf Streak and Spot Blotch in spring wheat landraces

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bacterial leaf streak (BLS), caused by Xanthomonas translucens pv. undulosa, and spot blotch (SB), caused by Cochliobolus sativus are two major diseases of wheat (Triticum aestivum L.). Planting resistant cultivars is the best approach to manage these diseases and identifying new sources of resistan...