Sample records for cv africana para

  1. Africana Digitization Project

    NSDL National Science Digital Library

    Produced by the Digital Content Group at the University of Wisconsin Library, the Africana Digitization Project provides a excellent template for further projects to make works dealing with Africa more accessible to researchers and other interested parties. Currently, there are eight works available for browsing on the site. These important resources include Andre Alvares Almada's _Brief Treatise on the rivers of Guinea_ from 1594, P.E.H. Hair's _Barbot's West African vocabularies of 1680_ from 1992, and Manuel Alvares's account of travels through modern-day Ethiopia from 1615. All of the works here are in English, and can be searched individually using the online search engine provided. For persons doing work in the field of African history, or those interested in reading travel narratives from Europeans, this repository will prove to be a helpful find. [KMG

  2. Cytotoxic glucosphingolipid from Celtis Africana

    PubMed Central

    Perveen, Shagufta; Al-Taweel, Areej Mohammad; Fawzy, Ghada Ahmed; El-Shafae, Azza Muhammed; Khan, Afsar; Proksch, Peter

    2015-01-01

    Background: Literature survey proved the use of the powdered sun-dried bark and roots of Celtis africana for the treatment of cancer in South Africa. Objective: The aim of this study was to do further isolation work on the ethyl acetate fraction and to investigate the cytotoxic activities of the various fractions and isolated compound. Materials and Methods: Cytotoxicity of petroleum ether, chloroform, ethyl acetate, n-butanol fractions and compound 1 were tested on mouse lymphoma cell line L5178Y using the microculture tetrazolium assay. Results: One new glucosphingolipid 1 was isolated from the aerial parts of C. africana. The structure of the new compound was determined by extensive analysis by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and mass spectrometry. The ethyl acetate fraction and compound 1 showed strong cytotoxic activity with an EC50 value of 8.3 ?g/mL and 7.8 ?g/mL, respectively, compared with Kahalalide F positive control (6.3 ?g/mL). Conclusion: This is the first report of the occurrence of a cytotoxic glucosphingolipid in family Ulmaceae. PMID:26109753

  3. Africana.com: The Digital Bridge

    NSDL National Science Digital Library

    Africana.com was founded in 1999 to present "information and commentary reflecting the diverse concerns of people of African descent." Designed by Harvard Square Netcasting, with Harvard University professors Henry Louis Gates and Anthony Appiah on the its Board of Directors, Africana.com provides a broad spectrum of information from scholarship to lifestyle articles and headline news. Several features boost the interactivity of the site: a free email service for registrants, a quiz (AfriQuest), and Radio Africana, listings of radio stations worldwide webcasting Black music. There are also links to related Web resources, a media center (primarily books and CDs for purchase), and the Adopt-A-School philanthropy program.

  4. Brain of the African Elephant (Loxodonta africana): Neuroanatomy

    E-print Network

    Allman, John M.

    Brain of the African Elephant (Loxodonta africana): Neuroanatomy From Magnetic Resonance Images images of the brain of an adult Afri- can elephant, Loxodonta africana, in the axial and parasagittal ) and of the neocortical and cerebellar gray and white matter. The white matter-to-gray matter ratio in the elephant neo

  5. Rules of habitat use by elephants Loxodonta africana in southern Africa: insights for regional management

    E-print Network

    Pretoria, University of

    Rules of habitat use by elephants Loxodonta africana in southern Africa: insights for regional in southern Africa are concerned that continually increasing elephant populations will degrade ecosystems africana, Maputo Elephant Reserve, optimal foraging, southern Africa, Tembe Elephant Park. This paper

  6. Extraction and utilization of breadfruit seed oil ( Treculia africana)

    Microsoft Academic Search

    V. I. E. Ajiwe; C. A. Okeke; H. U. Agbo

    1995-01-01

    Oil was extracted from crushed breadfruit (Treculia africana) seeds by Soxhlet. The yield of the oil was 20·83 ± 0·57%. Tests showed the oil to be a semi-drying oil which is unsaturated, with a high saponification value, acidic and requires purification. The oil could be used for making soap, hair shampoo and alkyd resin.

  7. Fermentation of Agave tequilana juice by Kloeckera africana: influence of amino-acid supplementations.

    PubMed

    Valle-Rodríguez, Juan Octavio; Hernández-Cortés, Guillermo; Córdova, Jesús; Estarrón-Espinosa, Mirna; Díaz-Montaño, Dulce María

    2012-02-01

    This study aimed to improve the fermentation efficiency of Kloeckera africana K1, in tequila fermentations. We investigated organic and inorganic nitrogen source requirements in continuous K. africana fermentations fed with Agave tequilana juice. The addition of a mixture of 20 amino-acids greatly improved the fermentation efficiency of this yeast, increasing the consumption of reducing sugars and production of ethanol, compared with fermentations supplemented with ammonium sulfate. The preference of K. africana for each of the 20 amino-acids was further determined in batch fermentations and we found that asparagine supplementation increased K. africana biomass production, reducing sugar consumption and ethanol production (by 30, 36.7 and 45%, respectively) over fermentations supplemented with ammonium sulfate. Therefore, asparagine appears to overcome K. africana nutritional limitation in Agave juice. Surprisingly, K. africana produced a high concentration of ethanol. This contrasts to poor ethanol productivities reported for other non-Saccharomyces yeasts indicating a relatively high ethanol tolerance for the K. africana K1 strain. Kloeckera spp. strains are known to synthesize a wide variety of volatile compounds and we have shown that amino-acid supplements influenced the synthesis by K. africana of important metabolites involved in the bouquet of tequila. The findings of this study have revealed important nutritional limitations of non-Saccharomyces yeasts fermenting Agave tequilana juice, and have highlighted the potential of K. africana in tequila production processes. PMID:21761236

  8. How to Write a CV

    NSDL National Science Digital Library

    2011-04-14

    This resource provides suggestions on how to write a CV (curriculum vitae). This resource provides suggestions on how to focus your CV for different positions one may apply to in academia. The resource also provides an example CV.

  9. Antioxidant and urease inhibitory C-glycosylflavonoids from Celtis africana

    Microsoft Academic Search

    Shagufta Perveen; Azza Muhammed El-Shafae; Areej Al-Taweel; Ghada Ahmed Fawzy; Abdul Malik; Nighat Afza; Mehreen Latif; Lubna Iqbal

    2011-01-01

    Two new C-glycosylflavonoids celtisides A (1) and B (2) have been isolated from n-butanol-soluble fraction of Celtis africana, along with five known C-glycosylflavonoids vitexin (3), orientin (4), isoswertiajaponin (5), isoswertisin (6), and 2?-O-rhamnosyl vitexin (7) reported for the first time from this species. Their structures were assigned from 1D and 2D NMR spectra. These compounds were investigated for biological activities

  10. Reproductive strategies of the kangaroo leech, Marsupiobdella africana (Glossiphoniidae)

    PubMed Central

    Kruger, Natasha; Du Preez, Louis

    2015-01-01

    The Kangaroo Leech, Marsupiobdella africana, is a hermaphroditic organism, with insemination taking place by the planting of a spermatophore on another leech. Spermatophores are mostly planted on the anterior of the recipient leech, but not always. Several spermatophores may be planted by different leeches on a single recipient. The spermatophore consists of two side by side lobes. Within minutes from planting of the spermatophore, the contents are squeezed out and into the body of the recipient. Sperm are believed to find the way to the ova by following chemical cues. Kangaroo Leeches display advanced parental care by transferring fertilized eggs from the reproductive opening to a brood pouch on the ventral side. Fully developed leeches may copulate after detaching from the amphibian host Xenopus laevis, or from the Cape River Crab Potamonautes perlatus with which it maintains a phoretic association. PMID:25830114

  11. Year Action IB # Jig HAL CP HAL CV Trawl CP Trawl CV

    E-print Network

    Year Action IB # Jig Pot Gear (CP and CV) HAL CP HAL CV Trawl CP Trawl CV TOTAL TAC ABC OFL 396 5,841 Year Action IB # Jig Pot Gear (CV and CP) HAL CP HAL CV 50 HAL CV CP Trawl = Overfishing Level CV = Catcher Vessel CP = Catcher Processor Rockfish Program Cooperatives this allocation

  12. Segmentação, Indexação e Recuperação de Vídeo Utilizando OpenCV

    Microsoft Academic Search

    Thiago Teixeira Santos; Carlos Hitoshi Morimoto

    Resumo: Os constantes avanços em poder de processamento, capacidade de armazenamento, e largura de banda tornaram possível ao usuário de com- putadores o acesso, manipulação e edição de imagens e vídeos digitais. Nesse tutorial, vamos descrever alguns algoritmos de visão computacional para a seg- mentação, indexação e recuperação de vídeo utilizando o OpenCV para que, ao final do tutorial, você

  13. CV-990 LSRA

    NASA Technical Reports Server (NTRS)

    1992-01-01

    A NASA CV-990, modified as a Landing Systems Research Aircraft (LSRA), is serviced on the ramp at NASA's Dryden Flight Research Center, Edwards, California, before a test of the space shuttle landing gear system. The space shuttle landing gear test unit, operated by a high-pressure hydraulic system, allowed engineers to assess and document the performance of space shuttle main and nose landing gear systems, tires and wheel assemblies, plus braking and nose wheel steering performance. The series of 155 test missions for the space shuttle program provided extensive data about the life and endurance of the shuttle tire systems and helped raise the shuttle crosswind landing limits at Kennedy.

  14. The influence of culture conditions on mycorrhiza formation between the ectomycorrhizal fungus Pisolithus sp. and Afzelia africana Sm. seedlings

    Microsoft Academic Search

    A. M. Bâ; J. Garbaye; J. Dexheimer

    1994-01-01

    Interactions between an isolate of the ectomycorrhizal fungus Pisolithus sp. and Afzelia africana Sm. seedlings were studied at the structural and ultrastructural levels. Several different conditions were tested with or without sugar and in a sterile or nonsterile medium. In the growth cabinet, the A. africana\\/Pisolithus sp. interactions did not produce ectomycorrhizas. A fungal sheath was formed but no Hartig

  15. CV 100--Still Going Strong

    ERIC Educational Resources Information Center

    Abrahams, A. L.

    1976-01-01

    Describes results of a study that used CV 100, a fuel additive for use in oil-fired heating systems, on a trial basis in 12 Ontario schools. The test showed an average 12 percent reduction in fuel costs in the schools using CV 100. (JG)

  16. Solutions for elephant Loxodonta africana crop raiding in northern Botswana: moving away from symptomatic approaches

    E-print Network

    Pretoria, University of

    Solutions for elephant Loxodonta africana crop raiding in northern Botswana: moving away from Conflict between people and elephants in Africa is widespread yet many solutions target the symptoms of the problem need to be examined. Here we examine factors underlying spatial use by elephants and people along

  17. Effect of storage temperature and sphacellum age on C. africana conidia survival

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sorghum ergot, caused by Claviceps africana Frederickson, Mantle & de Milliano, is a disease that poses a serious treat to sorghum, especially in hybrid seed production. The initial sign of the disease is called a sphacelium. Sphacelia containing macroconidia could play a role in the survival of t...

  18. A review of the status of forest elephants Loxodonta africana in Central Africa

    Microsoft Academic Search

    R. F. W. Barnes; A. Blom; M. P. T. Alers

    1995-01-01

    The status of elephants Loxodonta africana in the central African forests in 1989 is reviewed. These elephants accounted for about one-third of the continental total. However, there are few data on numbers, for only one of the six countries in the region has been censused. The estimates for the other countries came from a limited set of dung-counts or a

  19. Cultural and Agenda Conflicts in Academia: Critical Issues for Africana Women's Studies.

    ERIC Educational Resources Information Center

    Hudson-Weems, Clenora

    1989-01-01

    Suggests that women of African descent should name and define their unique movement "Africana Womanism" to distinguish it from limitations implicit in the notion of Black feminism. The movement advocates the rejection of all vestiges of White patriarchy and seeks to combat the triple oppressions of racism, classism, and sexism. (AF)

  20. The Center for Africana Studies at The Johns Hopkins University Invites Applications for the

    E-print Network

    Niebur, Ernst

    The Center for Africana Studies at The Johns Hopkins University Invites Applications for the 2011 University in conjunction with the Zanvyl Krieger School of Arts and Sciences is pleased to announce a one in other parts of the globe (i.e. the African Diaspora) are encouraged to apply. The field

  1. Male African elephants (Loxodonta africana) queue when the stakes are high

    Microsoft Academic Search

    C. E. OConnell-Rodwell; J. D. Wood; C. Kinzley; T. C. Rodwell; C. Alarcon; S. K. Wasser; R. Sapolsky

    2011-01-01

    Linear dominance hierarchies are thought to form within groups of social animals to minimize conflict over access to resources. Dominance in both male and female African elephants (Loxodonta africana) is based mostly on intrinsic factors relating to age, and dominance hierarchies have been described within and between family groups of females. Very little is reported about male elephant social structure

  2. Antiphonal vocal bouts associated with departures in free-ranging African elephant family groups (Loxodonta africana)

    Microsoft Academic Search

    C. E. OConnell-Rodwell; J. D. Wood; M. Wyman; S. Redfield; S. Puria; L. A. Hart

    2012-01-01

    We describe the interactive nature of vocalizations emitted by African elephant (Loxodonta africana) family groups while visiting waterholes. Despite being in close visual contact with each other, individuals called interactively within a series of overlapping or antiphonal vocal bouts that increased significantly while departing from the waterhole. After initiating departure from the waterhole, elephants in this study increased their calling

  3. Performance of African breadfruit ( Treculia africana Decne) seed flour in bread making

    Microsoft Academic Search

    Sunday Y. Giami; Tamunosaki Amasisi

    2003-01-01

    Replacing wheat flour (WHF) with flour (ABF) from roasted African breadfruit (Treculia africana Decne) seeds at levels of 0-25% was investigated for its effect on bread making properties. Resistance to extension (R), extensibility (E) and mechanical work for deformation (W) of doughs prepared from the blends were studied using a Chopin alveograph. Loaves were prepared using the straight-dough bread baking

  4. Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC.

    PubMed Central

    Agyare, Christian; Dwobeng, Anita Serwaa; Agyepong, Nicholas; Boakye, Yaw Duah; Mensah, Kwesi Boadu; Ayande, Patrick George; Adarkwa-Yiadom, Martin

    2013-01-01

    Microbial infections of various types of wounds are a challenge to the treatment of wounds and wound healing. The study was to investigate antimicrobial and antioxidant properties of methanol leaf and stem bark extracts of Kigelia africana and methanol leaf and root extracts of Strophanthus hispidus and also to determine wound healing properties of the extracts. The antimicrobial activities of the methanol extracts were determined against two Gram-positive and two Gram-negative bacteria and a fungus using agar diffusion and micro-dilution methods. The antioxidant activity was determined using 1,1-diphenyl-2-picryl–hydrazyl (DPPH) method. The influence of the extracts on rate of wound closure was investigated using the excision wound model and histopathological investigation of treated and untreated wound tissues performed. The MICs of leaf extract of K. africana against test organisms were 2.5–7.5?mg/mL and stem bark extract were 2.25–7.5?mg/mL. The leaf extract of S. hispidus had MIC range of 2.5–7.5?mg/mL and 2.5–10?mg/mL for root extract. The IC50 of leaf and stem bark extracts of K. africana were 56.9 and 13.7??g/mL, respectively and leaf and root of S. hispidus were 49.8 and 45.1??g/mL, respectively. K. africana extracts (7.5% w/w) showed significant (P < 0.05) wound contraction at day 7 with 72% of wound closure whiles significant (P < 0.05) wound contractions were observed on day 11 for stem bark of K. africana, leaf and root extracts of S. hispidus. Wound tissues treated with the extracts showed improved collagenation, re-epitheliazition and rapid granulation formation compared with untreated wound tissues. The extracts were found to contain alkaloids, saponins, tannins, flavonoids, carbohydrates, and sapogenetic glycosides. The HPLC finger-printing of the extracts were developed. The leaf, stem bark and root extracts of K. africana and S. hispidus exhibited antimicrobial, antioxidant, and enhanced wound healing properties and these may justify the medicinal uses of the plants for treatment of microbial infections and wounds. PMID:23662099

  5. Medicinal Plants Used in Wound Care: A Study of Prosopis africana (Fabaceae) Stem Bark

    PubMed Central

    Ezike, A. C.; Akah, P. A.; Okoli, C. O.; Udegbunam, S.; Okwume, N.; Okeke, C.; Iloani, O.

    2010-01-01

    The effects of the methanol extract of the stem bark of Prosopis africana (Guill., Perrott. and Rich.) Taubert (Fabaceae) on bleeding/clotting and coagulation time, excision and dead space wounds were studied in rats. Also, the extract was subjected to antibacterial, and acute toxicity and lethality (LD50) tests. The extract significantly (P<0.05) reduced bleeding/clotting and coagulation time in rats. It also reduced epithelialization period of excision wounds in rats and inhibited the growth of laboratory strains of Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Pseudomonas aeruginosa and Klebsiella pneumoniae to varying extents. Acute toxicity and lethality (LD50) test on the extract established an LD50 of 774 mg/kg (i.p) in mice while phytochemical analysis gave positive reactions for alkaloids, saponins, tannins, flavonoids, steroids, terpenoids and carbohydrates. The results of this study demonstrate the beneficial effects of the stem bark of P. africana in wound care. PMID:21188042

  6. [Chemical and DNA analyses for the products of a psychoactive plant, Voacanga africana].

    PubMed

    Kikura-Hanajiri, Ruri; Maruyama, Takuro; Miyashita, Akinori; Goda, Yukihiro

    2009-08-01

    Voacanga africana (Apocynaceae) is a small tropical African tree. The root bark and seeds of this tree contain a number of alkaloids, including ibogaine (a hallucinogenic/aphrodisiac compound in bark), tabersonine (a major constituteent of seeds) and other voacanga alkaloids, traditionally used in Africa for religious purposes. Recently, some kinds of products containing this plant (root bark and seeds) have been distributed in the drug market in expectation of its hallucinogenic/aphrodisiac effects. There has been no report that has discussed quantitative analyses of these alkaloids in the products and their botanical origins. In this study, to investigate the trend of such a non-controlled psychotropic plant of abuse, a simultaneous analytical method was developed using LC/MS for the voacanga alkaloids including ibogaine and tabersonine in the commercial products of V. africana. Moreover, the botanical origins of these products were investigated by DNA analyses. As a result of the LC/MS analyses, the products were classified into two chemical types; an ibogaine-type and a tabersonine-type. The samples of the ibogaine-type contain ibogaine (0.05-0.6%) and other voacanga alkaloids; voacamine, voacamidine and voacangine, while those of the tabersonine-type mainly contain tabersonine (0.6-1.6%). The sequence analyses of chloroplast DNA, trnL-F region suggested that most of the products were derived from V. africana or closely related plants. They were classified into four genotypes based on nucleotide sequence of the trnL-F IGS region. The proposed methods of chemical and DNA analyses would be useful for investigating the trend in the distribution of the products of V. africana. PMID:19652504

  7. Introduction to OpenCV David Stavens

    E-print Network

    Thrun, Sebastian

    1 Introduction to OpenCV David Stavens Stanford Artificial Intelligence Lab Tonight we'll code extensively in the 223b competition.) Plan OpenCV Basics What is it? How do you get started with it? Feature Finding and Optical Flow A brief mathematical discussion. OpenCV Implementation of Optical Flow Step

  8. Open CV intro Aside: Histogram Equalization

    E-print Network

    Kosecka, Jana

    : A fully functional sparse optical flow algorithm! Plan ·! OpenCV Basics ­! What is it? ­! How do you get started with it? ·! Feature Finding and Optical Flow ­! A brief mathematical discussion. ·! OpenCV Implementation of Optical Flow ­! Step by step. #12;What is OpenCV? ·! Created/Maintained by Intel/Willow Garage

  9. Nutrient Composition of African Breadfruit (Treculia africana) Seed Hull and its Use in Diets for the African Giant Land Snail, Archachatina marginata

    Microsoft Academic Search

    2007-01-01

    A feeding trial was conducted to evaluate the use of Treculia africana seed hull meal (TASHM) as replacement for maize in diets for the African giant land snail, Archachatina marginata. Seeds of T. africana were parboiled, dehulled, milled and used to replace maize at 0%, 15%, 30% and 45% (diets I, II, III and IV, respectively) in isoproteic diets (20%

  10. CV

    E-print Network

    Graduate Students ... 9. Non-doubling Ahlfors measures, perimeter measures, and the characterization of .... Exciting Science @ Purdue (School of Science recruitment event), Organizer, April 2003 and April 2004. ... Strategic Planning Oversight Committee (2009-10) ... University Grade Appeal Committee (2012-

  11. CV

    E-print Network

    2015-04-13

    Interests. Linear Algebra, Combinatorics, and Theoretical Computer Science. Education. Rutgers, The State University of New Jersey–New Brunswick, NJ ... Thesis Topic: Computational Aspects of the Combinatorial Nullstellensatz Method.

  12. Mac Strelioff: CV 1 Mac Strelioff

    E-print Network

    Loudon, Catherine

    Mac Strelioff: CV 1 Mac Strelioff 1100 Olive Drive, Davis, CA 95616 Cell: 1 (619) 699-9845 Email: MacStrelioff@UCDavis.edu Education University of California at Davis, Davis, CA August 2012 ­ June affiliate member of Association for Psychological Science (APS). #12;Mac Strelioff: CV 2 Research; Poster

  13. Bass CV, page 1 Curriculum Vitae

    E-print Network

    Bass, Brenda L.

    Bass CV, page 1 Curriculum Vitae Brenda L. Bass Personal Address: Department of Biochemistry-5650 Telephone: (801) 581-4884 Email: bbass@biochem.utah.edu Website: http://www.biochem.utah.edu/bass Colorado Springs, Colorado #12;Bass CV, page 2 Honors 2011 AAAS Fellow (elected) 2011 NIH Director

  14. Two jacalin-related lectins from seeds of the African breadfruit (Treculia africana L.).

    PubMed

    Shimokawa, Michiko; Nsimba-Lubaki, Shadrack Makuta; Hayashi, Namiko; Minami, Yuji; Yagi, Fumio; Hiemori, Keiko; Tateno, Hiroaki; Hirabayashi, Jun

    2014-01-01

    Two jacalin-related lectins (JRLs) were purified by mannose-agarose and melibiose-agarose from seeds of Treculia africana. One is galactose-recognizing JRL (gJRL), named T. africana agglutinin-G (TAA-G), and another one is mannose-recognizing JRL (mJRL), TAA-M. The yields of the two lectins from the seed flour were approximately 7.0 mg/g for gJRL and 7.2 mg/g for mJRL. The primary structure of TAA-G was determined by protein sequencing of lysyl endopeptic peptides and chymotryptic peptides. The sequence identity of TAA-G to other gJRLs was around 70%. Two-residue insertion was found around the sugar-binding sites, compared with the sequences of other gJRLs. Crystallographic studies on other gJRLs have shown that the primary sugar-binding site of gJRLs can accommodate Gal, GalNAc, and GalNAc residue of T-antigen (Gal?1-3GalNAc?-). However, hemagglutination inhibition and glycan array showed that TAA-G did not recognize GalNAc itself and T-antigen. TAA-G preferred melibiose and core 3 O-glycan. PMID:25155899

  15. Mortality trends in the African cherry ( Prunus africana) and the implications for colobus monkeys ( Colobus guereza) in Kakamega Forest, Kenya

    Microsoft Academic Search

    Peter J. Fashing

    2004-01-01

    Prunus africana (Hook.f.) Kalkm. is a secondary forest canopy tree species that has been declining over much of its geographical range in sub-Saharan Africa during recent decades due to unsustainable harvesting of its bark for the international medicinal plant trade. One of the locations where the species is experiencing rapid mortality is Isecheno study site in the Kakamega Forest, Kenya

  16. Growth rates, biomass and distribution of selected woody plant roots in Burkea africana-Ochna pulchra savanna

    Microsoft Academic Search

    M. C. Rutherford

    1983-01-01

    Woody plants in an African Burkea africana-Ochna pulchra savanna on deep sandy soil were found to have characteristically bimorphic root systems. The shallow lateral root component was often well developed and roots extended up to seven times the extent of the plant canopy in several species. Exponential tapering of lateral roots was found in Terminalia sericea. The wide-ranging roots, together

  17. Characterisation of the sympathetic nervous system of Asian ( Elephas maximus) and African ( Loxodonta africana) elephants based on urinary catecholamine analyses

    Microsoft Academic Search

    M. Dehnhard

    2007-01-01

    Assessing the welfare status of captive animals using non-invasive measurements of hormones is of growing interest because this can serve as an effective tool to facilitate the optimization of environmental and husbandry conditions. Both the African elephant (Loxodonta africana) and the Asian elephant (Elephas maximus) exhibit extremely low breeding success in captivity, and because elevated levels of stress may negatively

  18. Endocrinology of the ovarian cycle and pregnancy in the Asian ( Elephas maximus) and African ( Loxodonta africana) elephant

    Microsoft Academic Search

    J. K Hodges

    1998-01-01

    In reviewing the literature, this paper assesses the current level of understanding of the hormonal control of the ovarian cycle and pregnancy in the elephant. Data are compiled from two genera, Elephas maximus and Loxodonta africana, recognizing differences where known to exist. Measurements of various steroid and peptide hormones, but most notably immunoreactive progesterone (iP), indicate an overall cycle length

  19. Effect of heat processing on in vitro protein digestibility and some chemical properties of African breadfruit ( Treculia africana Decne) seeds

    Microsoft Academic Search

    Sunday Y. Giami; Matthew N. Adindu; Alexandria D. Hart; Emmanuel O. Denenu

    2001-01-01

    The effects of dry heat (roasting) and moist heat (boiling) on in vitro protein digestibility, protein fractions and other chemical properties of African breadfruit (Treculia africana Decne) seed that affect their utilization as a source of human food were investigated. Chemical analyses showed that the crude protein and fat contents of the unprocessed (raw) seeds were 20.1% and 13.7%, respectively.

  20. Small-plot studies of Desmodium intortum (Mill.) Urb. cv. ‘Greenleaf’, D. uncinatum (Jacq.) DC. cv. ‘Silverleaf’, and Lotononis bainesii Baker cv. ‘Miles’, in Northland, New Zealand

    Microsoft Academic Search

    G. J. Goold

    1978-01-01

    Establishment and subsequent dry matter productivity of three subtropical legume species Desmodium intortum cv. ‘Greenleaf’, D. uncinatum cv. ‘Silverleaf’, and Lotononis bainesii cv. ‘Miles’ were compared with that of Lotus pendunculatus cv. ‘Commercial’ at two sites in Northland from 1971–74. Late December sowings resulted in successful establishment of both Desmodium species at the two sites. The establishment of Lotononis and

  1. Conservation Priorities for Prunus africana Defined with the Aid of Spatial Analysis of Genetic Data and Climatic Variables

    PubMed Central

    Vinceti, Barbara; Loo, Judy; Gaisberger, Hannes; van Zonneveld, Maarten J.; Schueler, Silvio; Konrad, Heino; Kadu, Caroline A. C.; Geburek, Thomas

    2013-01-01

    Conservation priorities for Prunus africana, a tree species found across Afromontane regions, which is of great commercial interest internationally and of local value for rural communities, were defined with the aid of spatial analyses applied to a set of georeferenced molecular marker data (chloroplast and nuclear microsatellites) from 32 populations in 9 African countries. Two approaches for the selection of priority populations for conservation were used, differing in the way they optimize representation of intra-specific diversity of P. africana across a minimum number of populations. The first method (S1) was aimed at maximizing genetic diversity of the conservation units and their distinctiveness with regard to climatic conditions, the second method (S2) at optimizing representativeness of the genetic diversity found throughout the species’ range. Populations in East African countries (especially Kenya and Tanzania) were found to be of great conservation value, as suggested by previous findings. These populations are complemented by those in Madagascar and Cameroon. The combination of the two methods for prioritization led to the identification of a set of 6 priority populations. The potential distribution of P. africana was then modeled based on a dataset of 1,500 georeferenced observations. This enabled an assessment of whether the priority populations identified are exposed to threats from agricultural expansion and climate change, and whether they are located within the boundaries of protected areas. The range of the species has been affected by past climate change and the modeled distribution of P. africana indicates that the species is likely to be negatively affected in future, with an expected decrease in distribution by 2050. Based on these insights, further research at the regional and national scale is recommended, in order to strengthen P. africana conservation efforts. PMID:23544118

  2. FATAL ENCEPHALOMYOCARDITIS VIRUS INFECTION IN AN AFRICAN SAVANNA ELEPHANT (LOXODONTA AFRICANA) IN A FRENCH ZOO.

    PubMed

    Lamglait, Benjamin; Joris, Antoine; Romey, Aurore; Bakkali-Kassimi, Labib; Lemberger, Karin

    2015-06-01

    A fatal case of encephalomyocarditis virus (EMCV) involving an African elephant ( Loxodonta africana ) occurred in November 2013 at the Réserve Africaine de Sigean, France. An adult female was found dead without any preliminary symptoms. Gross pathologic changes consisted of petechiae and hemorrhages on mucosae and internal organs, abundant transudate in the abdominal and pericardial cavities, and myocarditis. Histopathologic examination showed extensive degeneration and necrosis of ventricular cardiomyocytes with concurrent lymphoplasmocytic and eosinophilic infiltrate. An EMCV was isolated from several organs and considered the causative agent of the myocarditis. The same strain of virus was also isolated in rodents captured on zoo premises and considered to be the reservoir of the virus. To the authors' knowledge, this is the first EMCV case in a captive African elephant in Europe. PMID:26056902

  3. Strangulating intestinal obstructions in four captive elephants (Elephas maximus and Loxodonta africana).

    PubMed

    Wiedner, Ellen B; Peddie, James; Peddie, Linda Reeve; Abou-Madi, Noha; Kollias, George V; Doyle, Charles; Lindsay, William A; Isaza, Ramiro; Terrell, Scott; Lynch, Tim M; Johnson, Kari; Johnson, Gary; Sammut, Charlie; Daft, Barbara; Uzal, Francisco

    2012-03-01

    Three captive-born (5-day-old, 8-day-old, and 4-yr-old) Asian elephants (Elephas maximus) and one captive-born 22-yr-old African elephant (Loxodonta africana) from three private elephant facilities and one zoo in the United States presented with depression, anorexia, and tachycardia as well as gastrointestinal signs of disease including abdominal distention, decreased borborygmi, tenesmus, hematochezia, or diarrhea. All elephants showed some evidence of discomfort including agitation, vocalization, or postural changes. One animal had abnormal rectal findings. Nonmotile bowel loops were seen on transabdominal ultrasound in another case. Duration of signs ranged from 6 to 36 hr. All elephants received analgesics and were given oral or rectal fluids. Other treatments included warm-water enemas or walking. One elephant underwent exploratory celiotomy. Three animals died, and the elephant taken to surgery was euthanized prior to anesthetic recovery. At necropsy, all animals had severe, strangulating intestinal lesions. PMID:22448519

  4. Oligosaccharides produced by submerged cultures of Claviceps africana and Claviceps sorghi.

    PubMed

    Flieger, M; Kantorová, M; Halada, P; Kuzma, M; Pazoutová, S; Stod?lková, E; Kolínská, R

    2005-01-01

    Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains. PMID:16295657

  5. CV Medina, page 1 EDEN MEDINA

    E-print Network

    Menczer, Filippo

    for Social Informatics Adjunct Associate Professor of History, College of ArtsCV Medina, page 1 EDEN MEDINA SCHOOL OF INFORMATICS AND COMPUTING INDIANA: (812) 856-1871 FAX: (812) 856-1995 EDENM@INDIANA.EDU HTTP://INFORMATICS

  6. Tutorials on Matlab, OpenCV, and Computer Science Department

    E-print Network

    Jiang, Hao

    Tutorials on Matlab, OpenCV, and SDL Computer Science Department Hao Jiang #12;Matlab · Matlab. · Rewrite bottleneck procedures using c. ­ System(`c_exe'); ­ Compile the function using mex. #12;OpenCV #12;OpenCV · OpenCV is a C library for image processing and computer vision. http://www.intel.com/technology/computing/opencv

  7. Anti-spasmodic action of crude methanolic extract and a new compound isolated from the aerial parts of Myrsine africana

    PubMed Central

    2011-01-01

    Background Myrsine africana is an herbaceous plant that is traditionally used as appetizer and carminative. Locally, it is used for the treatment of pulmonary tuberculosis, rheumatism and diarrhea by healers. The aims of the current study were to screen the crude methanol extract obtained from the aerial parts (leaves and stem) of M. africana, for antispasmodic actions on isolated tissues and further to subject the ethyl acetate (EtOAc) fraction of plant to column chromatography for isolation of pure compounds. Methods The antispasmodic action of the crude methanol extract was measured on the spontaneous rabbit's jejunum preparations at concentration 0.01, 0.03, 0.1, 0.3, 1.0, 5.0 and 10.0 mg/ml. The crude extract was also applied, in similar concentrations, on KCl (80 mM) induced contractions to explain its possible mode of action. Results A new compound Myrsigenin was isolated from the EtOAc fraction of M. africana. The structure of the compound was identified with the help of 13C-NMR, 1H-NMR, HMBC, HMQC, NOESY and COSY. The plant crude methanol extract showed a significant antispasmodic action on rabbit jejunum and abolished the tissue contraction completely at concentration of 5.0 mg/ml. Conclusion The study concludes that the methanol crude extract of aerial parts of M. africana has antispasmodic action possibly through the calcium channel blocking mechanisms. A new compound Myrsigenin was isolated from the EtOAc fraction of the plant. PMID:21733176

  8. Comparisons of State and Likelihood of Performing Chemosensory Event Behaviors in Two Populations of African Elephants ( Loxodonta africana )

    Microsoft Academic Search

    Bruce A. Schulte; Kathryn R. Bagley; Matthew Groover; Helen Loizi; Christen Merte; Jordana M. Meyer; Erek Napora; Lauren Stanley; Dhaval K. Vyas; Kimberly Wollett; Thomas E. Goodwin; L. E. L. Rasmussen

    The demonstration of a species-level chemical signal assumes that the same chemical signal serves a similar purpose across\\u000a the range of the species. Yet, the response to putative chemical signals varies with social setting, environmental conditions,\\u000a age, sex, and reproductive status of the individuals. Through observations and biological assays with African elephants (Loxodonta africana), we evaluated variation in state behaviors

  9. Effect of alkaline treatment (‘akanwu’) and supplementary value of corn or crayfish on the protein quality of breadfruit (Treculia Africana)

    Microsoft Academic Search

    Ngozi Rose Nzomiwu

    1990-01-01

    The effect of treatment and supplementary value of corn (C) or crayfish (CR) on the protein quality of breadfruit (Treculia Africana) flours were studied in eighteen young rats. The 70?30 or 70?15?15 (Protein basis) combinations of breadfruit flours and corn or crayfish or both provided 1.6g N\\/100 g diet for the 35 day study. The addition of ‘akanwu’ to the

  10. Compositional, functional and storage properties of flours from raw and heat processed African breadfruit ( Treculia africana Decne) seeds

    Microsoft Academic Search

    Sunday Y. Giami; Matthew N. Adindu; Monday O. Akusu; Juliet N. T. Emelike

    2000-01-01

    African breadfruit (Treculia africana Decne) seeds were either boiled or roasted and then milled into flour. Chemical composition, functional properties and storage characteristics of raw and treated flours and the effect of partial proteolysis on selected functional properties of the raw flour were determined. Raw flour contained 20.1% crude protein, 2.5% total ash and 13.7% fat. Heat processing significantly (pp<0.05)

  11. 1/21/14 CV -Deines Research https://sites.google.com/site/deineslab/cv 1/6

    E-print Network

    1/21/14 CV - Deines Research https://sites.google.com/site/deineslab/cv 1/6 Andrew M. Deines and Systematics. 43:-- #12;1/21/14 CV - Deines Research https://sites.google.com/site/deineslab/cv 2/6 2005 Deines, Lodge DM. Influence of fishery effort and #12;1/21/14 CV - Deines Research https://sites.google.com

  12. Prunus africana (Hook.f.) Kalkman: the overexploitation of a medicinal plant species and its legal context.

    PubMed

    Bodeker, Gerard; van 't Klooster, Charlotte; Weisbord, Emma

    2014-11-01

    The linkage between herbal medicines and the sustainability of medical plants from which they are manufactured is increasingly being understood and receiving attention through international accords and trade labeling systems. However, little attention is paid to the fair trade aspects of this sector, including the issue of benefit-sharing agreements with traditional societies whose knowledge and resources are being exploited for commercial herbal medicine development and production. This article examines the case of Prunus africana (Hook.f.) Kalkman, from equatorial Africa. While the conservation and cultivation dimension of the trade in P. africana has been much discussed in literature, no research appears to have focused on the traditional resource rights and related ethical dimensions of this trade in traditional medicine of Africa. Serving as a cautionary tale for the unbridled exploitation of medicinal plants, the history of P. africana extraction is considered here in the context of relevant treaties and agreements existing today. These include the Nagoya Protocol, a supplementary agreement to the Convention on Biological Diversity, the Trade-Related Aspects of Intellectual Property Rights agreement from the World Trade Organization, and two African regional frameworks: the Swakopmund Protocol and the Organisation Africaine de la Propriété Intellectuelle Initiative. In the context of strengthening medicinal plant research in Africa, a novel international capacity-building project on traditional medicines for better public health in Africa will be discussed, illustrating how access and benefit sharing principles might be incorporated in future projects on traditional medicines. PMID:25225776

  13. Prunus africana (Hook.f.) Kalkman: The Overexploitation of a Medicinal Plant Species and Its Legal Context

    PubMed Central

    Bodeker, Gerard; Weisbord, Emma

    2014-01-01

    Abstract The linkage between herbal medicines and the sustainability of medical plants from which they are manufactured is increasingly being understood and receiving attention through international accords and trade labeling systems. However, little attention is paid to the fair trade aspects of this sector, including the issue of benefit-sharing agreements with traditional societies whose knowledge and resources are being exploited for commercial herbal medicine development and production. This article examines the case of Prunus africana (Hook.f.) Kalkman, from equatorial Africa. While the conservation and cultivation dimension of the trade in P. africana has been much discussed in literature, no research appears to have focused on the traditional resource rights and related ethical dimensions of this trade in traditional medicine of Africa. Serving as a cautionary tale for the unbridled exploitation of medicinal plants, the history of P. africana extraction is considered here in the context of relevant treaties and agreements existing today. These include the Nagoya Protocol, a supplementary agreement to the Convention on Biological Diversity, the Trade-Related Aspects of Intellectual Property Rights agreement from the World Trade Organization, and two African regional frameworks: the Swakopmund Protocol and the Organisation Africaine de la Propriété Intellectuelle Initiative. In the context of strengthening medicinal plant research in Africa, a novel international capacity-building project on traditional medicines for better public health in Africa will be discussed, illustrating how access and benefit sharing principles might be incorporated in future projects on traditional medicines. PMID:25225776

  14. The relationship between social behaviour and habitat familiarity in African elephants (Loxodonta africana).

    PubMed

    Pinter-Wollman, Noa; Isbell, Lynne A; Hart, Lynette A

    2009-03-22

    Social associations with conspecifics can expedite animals' acclimation to novel environments. However, the benefits gained from sociality may change as the habitat becomes familiar. Furthermore, the particular individuals with whom animals associate upon arrival at a new place, familiar conspecifics or knowledgeable unfamiliar residents, may influence the type of information they acquire about their new home. To examine animals' social dynamics in novel habitats, we studied the social behaviour of African elephants (Loxodonta africana) translocated into a novel environment. We found that the translocated elephants' association with conspecifics decreased over time supporting our hypothesis that sociality provides added benefits in novel environments. In addition, we found a positive correlation between body condition and social association, suggesting that elephants gain direct benefits from sociality. Furthermore, the translocated elephants associated significantly less than expected with the local residents and more than expected with familiar, but not necessarily genetically related, translocated elephants. The social segregation between the translocated and resident elephants declined over time, suggesting that elephants can integrate into an existing social setting. Knowledge of the relationship between sociality and habitat familiarity is highly important in our constantly changing world to both conservation practice and our understanding of animals' behaviour in novel environments. PMID:19129113

  15. The relationship between social behaviour and habitat familiarity in African elephants (Loxodonta africana)

    PubMed Central

    Pinter-Wollman, Noa; Isbell, Lynne A.; Hart, Lynette A.

    2008-01-01

    Social associations with conspecifics can expedite animals' acclimation to novel environments. However, the benefits gained from sociality may change as the habitat becomes familiar. Furthermore, the particular individuals with whom animals associate upon arrival at a new place, familiar conspecifics or knowledgeable unfamiliar residents, may influence the type of information they acquire about their new home. To examine animals' social dynamics in novel habitats, we studied the social behaviour of African elephants (Loxodonta africana) translocated into a novel environment. We found that the translocated elephants' association with conspecifics decreased over time supporting our hypothesis that sociality provides added benefits in novel environments. In addition, we found a positive correlation between body condition and social association, suggesting that elephants gain direct benefits from sociality. Furthermore, the translocated elephants associated significantly less than expected with the local residents and more than expected with familiar, but not necessarily genetically related, translocated elephants. The social segregation between the translocated and resident elephants declined over time, suggesting that elephants can integrate into an existing social setting. Knowledge of the relationship between sociality and habitat familiarity is highly important in our constantly changing world to both conservation practice and our understanding of animals' behaviour in novel environments. PMID:19129113

  16. Renaut bodies in nerves of the trunk of the African elephant, Loxodonta africana.

    PubMed

    Witter, Kirsti; Egger, Gunter F; Boeck, Peter

    2007-05-01

    Renaut bodies are loosely textured, cell-sparse structures in the subperineurial space of peripheral nerves, frequently found at sites of nerve entrapment. The trunk of the elephant is a mobile, richly innervated organ, which serves for food gathering, object grasping and as a tactile organ. These functions of the trunk lead to distortion and mechanical compression of its nerves, which can therefore be expected to contain numerous Renaut bodies. Samples of the trunk wall of an adult African elephant (Loxodonta africana) were examined histologically using conventional staining methods, immunohistochemistry, and lectin histochemistry. Architecture of nerve plexuses and occurrence of Renaut bodies in the elephant trunk were compared with those in tissues surrounding the nasal vestibule of the pig. Prominent nerve plexuses were found in all layers of the elephant trunk. Almost all (81%) nerve profiles contained Renaut bodies, a basophilic, discrete subperineurial layer resembling cushions around the nerve core. In contrast, Renaut bodies were seen in only 15% of nerve profiles in the porcine nasal vestibule. Within Renaut bodies, fusiform fibroblasts and round, ruff-like cells were placed into a matrix of acidic glycosaminoglycans with delicate collagen and very few reticular fibers. The turgor of this matrix is thought to protect nerves against compression and shearing strain. Renaut bodies are readily stained with alcian blue (pH 2.5) favorably in combination with immunohistochemical markers of nerve fibers. They should be regarded as a physiological response to repeated mechanical insults and are distinct from pathological alterations. alterations. PMID:17390335

  17. A Preliminary Analysis of the Immunoglobulin Genes in the African Elephant (Loxodonta africana)

    PubMed Central

    Guo, Yongchen; Bao, Yonghua; Wang, Hui; Hu, Xiaoxiang; Zhao, Zhihui; Li, Ning; Zhao, Yaofeng

    2011-01-01

    The genomic organization of the IgH (Immunoglobulin heavy chain), Ig? (Immunoglobulin kappa chain), and Ig? (Immunoglobulin lambda chain) loci in the African elephant (Loxodonta africana) was annotated using available genome data. The elephant IgH locus on scaffold 57 spans over 2,974 kb, and consists of at least 112 VH gene segments, 87 DH gene segments (the largest number in mammals examined so far), six JH gene segments, a single ?, a ? remnant, and eight ? genes (? and ? genes are missing, most likely due to sequence gaps). The Ig? locus, found on three scaffolds (202, 50 and 86), contains a total of 153 V? gene segments, three J? segments, and a single C? gene. Two different transcriptional orientations were determined for these V? gene segments. In contrast, the Ig? locus on scaffold 68 includes 15 V? gene segments, all with the same transcriptional polarity as the downstream J?-C? cluster. These data suggest that the elephant immunoglobulin gene repertoire is highly diverse and complex. Our results provide insights into the immunoglobulin genes in a placental mammal that is evolutionarily distant from humans, mice, and domestic animals. PMID:21364892

  18. A preliminary analysis of the immunoglobulin genes in the African elephant (Loxodonta africana).

    PubMed

    Guo, Yongchen; Bao, Yonghua; Wang, Hui; Hu, Xiaoxiang; Zhao, Zhihui; Li, Ning; Zhao, Yaofeng

    2011-01-01

    The genomic organization of the IgH (Immunoglobulin heavy chain), Ig? (Immunoglobulin kappa chain), and Ig? (Immunoglobulin lambda chain) loci in the African elephant (Loxodonta africana) was annotated using available genome data. The elephant IgH locus on scaffold 57 spans over 2,974 kb, and consists of at least 112 V(H) gene segments, 87 D(H) gene segments (the largest number in mammals examined so far), six J(H) gene segments, a single ?, a ? remnant, and eight ? genes (? and ? genes are missing, most likely due to sequence gaps). The Ig? locus, found on three scaffolds (202, 50 and 86), contains a total of 153 V(?) gene segments, three J(?) segments, and a single C(?) gene. Two different transcriptional orientations were determined for these V(?) gene segments. In contrast, the Ig? locus on scaffold 68 includes 15 V(?) gene segments, all with the same transcriptional polarity as the downstream J(?)-C(?) cluster. These data suggest that the elephant immunoglobulin gene repertoire is highly diverse and complex. Our results provide insights into the immunoglobulin genes in a placental mammal that is evolutionarily distant from humans, mice, and domestic animals. PMID:21364892

  19. Wild African elephants (Loxodonta africana) discriminate between familiar and unfamiliar conspecific seismic alarm calls.

    PubMed

    O'Connell-Rodwell, Caitlin E; Wood, Jason D; Kinzley, Colleen; Rodwell, Timothy C; Poole, Joyce H; Puria, Sunil

    2007-08-01

    The ability to discriminate between call types and callers as well as more subtle information about the importance of a call has been documented in a range of species. This type of discrimination is also important in the vibrotactile environment for species that communicate via vibrations. It has recently been shown that African elephants (Loxodonta africana) can detect seismic cues, but it is not known whether they discriminate seismic information from noise. In a series of experiments, familiar and unfamiliar alarm calls were transmitted seismically to wild African elephant family groups. Elephants respond significantly to the alarm calls of familiar herds (p=0.004) but not to the unfamiliar calls and two different controls, thus demonstrating the ability of elephants to discriminate subtle differences between seismic calls given in the same context. If elephants use the seismic environment to detect and discriminate between conspecific calls, based on the familiarity of the caller or some other physical property, they may be using the ground as a very sophisticated sounding board. PMID:17672633

  20. Nocardia africana sp. nov., a New Pathogen Isolated from Patients with Pulmonary Infections

    PubMed Central

    Hamid, Mohamed E.; Maldonado, Luis; Sharaf Eldin, Ghada S.; Mohamed, Maha F.; Saeed, Nageeb S.; Goodfellow, Michael

    2001-01-01

    Eight actinomycete strains, isolated from 8 out of 400 sputum samples examined, taken from patients with pulmonary diseases at the Chest Unit of Khartoum Teaching Hospital in the Sudan, were provisionally assigned to the genus Nocardia according to morphological criteria. These isolates were studied further in order to establish their taxonomic status. They were found to have morphological and chemical properties typical of nocardiae and formed a monophyletic clade in the 16S ribosomal DNA tree together with Nocardia vaccinii. The strains showed a unique pattern of phenotypic properties that distinguished them from representatives of recognized Nocardia species, including Nocardia vaccinii. The strains were considered to merit species status and were designated Nocardia africana sp. nov. The findings of the present study are consistent with the view that pulmonary nocardiosis may occur in a substantial proportion of patients who exhibit chronic lung diseases in African countries. It is important, therefore, that clinicians in such countries consider this condition, especially when patients with respiratory infections fail to respond to antitubercular therapy. PMID:11158119

  1. JEREMY HEIS CV, updated October 2010

    E-print Network

    Stanford, Kyle

    JEREMY HEIS CV, updated October 2010 Logic and Philosophy of Science Phone: 949-824-5442 University://www.lps.uci.edu/heis Employment 2007-present. Assistant Professor, Department of Logic and Philosophy of Science, University of Specialization History and Philosophy of Mathematics and Logic; Kant (theoretical philosophy); Early Analytic

  2. CV: J. Heintz. -1-James Heintz

    E-print Network

    Massachusetts at Amherst, University of

    CV: J. Heintz. -1- James Heintz Research Professor Political Economy Research Institute 418 N-413-577-0261 Date of birth: 25 March 1968 Current Position Research Professor (since Sept. 2011) Political Economy Economies 20: 67-103 (supplement 2). "The Structure of Employment, Globalization, and Economic Crises

  3. CV (Mercado). February 2005 NORMAN MERCADO SILVA

    E-print Network

    Mercado-Silva, Norman

    CV (Mercado). February 2005 NORMAN MERCADO SILVA Center for Limnology University of Wisconsin - Madison; 2001. PhD: Zoology and Limnology, Department of Zoology, University of Wisconsin ­ Madison, 2005 - Postdoctoral Associate. Center for Limnology (University of Wisconsin - Madison) 2004 - 05 Research Assistant

  4. Schultz CV page 1 STEPHEN SCHULTZ

    E-print Network

    Wirthlin, Michael J.

    communications for air-to-air, air-to-satellite, and air-to-ground. · Analyzed atmospheric propagation of lasers City, UT #12;Schultz CV page 2 · Analyzed the advantages and disadvantages of free space laser and stray light analysis on various passive infrared imaging missile seekers and active laser radar systems

  5. McGuire CV Page 1 of 5

    E-print Network

    McGuire, Jeff

    McGuire ­ CV Page 1 of 5 Jeffrey Joseph McGuire Associate Scientist, Department of Geology to their relatively simple geometrical and geological structure and the #12;McGuire ­ CV Page 2 of 5 preponderance

  6. McGuire CV Page 1 of 8

    E-print Network

    McGuire, Jeff

    McGuire ­ CV Page 1 of 8 Jeffrey Joseph McGuire Associate Scientist, Department of Geology to their relatively simple geometrical and geological structure and the #12;McGuire ­ CV Page 2 of 8 preponderance

  7. The OpenCV Library: Computing Optical Flow

    E-print Network

    Thrun, Sebastian

    1 The OpenCV Library: Computing Optical Flow David Stavens Stanford Artificial Intelligence Lab I optical flow algorithm! Plan OpenCV Basics What is it? How do we get started? Feature Finding and Optical Flow A brief mathematical discussion. OpenCV Implementation of Optical Flow Step by step. #12;3 What

  8. SeeingWithOpenCV FollowThatFace!

    E-print Network

    Oh, Paul

    CVtotrackataceonce you'vedetectedit. FaceTrackingin OpenCV Trackinga face is more drfficult thantrackinga strongly face by locatingit over and over In every frame, using the Haar detector "How OpenCV's FaceTrackerWorks," explainsthis algo- FIGURE1. OpenCV's face tracker in action, It's able to follow a face as it tilts to one

  9. What is a CV? Keeping it up to date Templates and tips Job applications Homework Preparing an Academic CV

    E-print Network

    Stockie, John

    it up to date Templates and tips Job applications Homework Required categories in a CV Complete name (noWhat is a CV? Keeping it up to date Templates and tips Job applications Homework Preparing Group, SFU 1/43 #12;What is a CV? Keeping it up to date Templates and tips Job applications Homework

  10. The spatial structure of hunter access determines the local abundance of forest elephants (Loxodonta africana cyclotis).

    PubMed

    Yackulic, Charles B; Strindberg, Samantha; Maisels, Fiona; Blake, Stephen

    2011-06-01

    In many previously remote regions in the world, increasing and often unregulated access is leading to dramatic increases in hunting pressure and declines in the densities of prey species, sometimes to the point of local extinction. Not surprisingly, numerous studies have found a correlation between the distance to the closest access point and prey densities. Here we hypothesized that, for many wide-ranging species, local abundances are reduced by hunting associated with multiple access points as opposed to just the closest access points. We also hypothesized that the distribution of hunter access determines both patterns of occupancy and abundance in occupied areas and that these two patterns (occupancy and abundance) respond to access at different spatial scales. Using data on the distribution of abundances of African forest elephant (Loxodonta africana cyclotis) in and around five national parks in Central Africa, we tested these hypotheses using a model comparison framework. We found that models including an index based on the distance to multiple roads outperformed models including other access-based covariates, including a model based on distance to the closest road only. We also found that models that allowed us to model occupancy and abundance separately outperformed simpler models. Occupancy responds to access at the same scale as previous estimates of average maximum displacement in the subspecies, while the scale of the response of abundance is more ambiguous, but appears to be greater. Lastly, we show that incorporating indices based on multiple access points and modeling abundance and occupancy has important practical consequences for our understanding of overall regional abundances and the distribution of abundances within regions. PMID:21774431

  11. Lack of spatial and behavioral responses to immunocontraception application in African elephants (Loxodonta africana).

    PubMed

    Delsink, Audrey K; Kirkpatrick, Jay; van Altena, J J; Bertschinger, Henk J; Ferreira, Sam M; Slotow, Robert

    2013-12-01

    Opinions are divided as to whether human intervention to control elephant (Loxodonta africana) population growth is desirable, partly because of elephant welfare concerns. Female contraception through immunization with porcine zona pellucida (PZP) proteins is viable. The effects of sustained use and application of the PZP vaccine on elephant behavioral and spatial responses were examined by evaluating herd ranging, fission-fusion dynamics, association patterns, and reproductive and sexual behaviors. Minimal change was anticipated as a result of long calf dependence on and association with cows, a reduced but not indefinite 0% growth rate and the known mechanism of action of PZP vaccines, and minimal expected change in resource requirements necessitating behavioral or spatial use adaptations. Although behavioral effects identified in previous hormonal contraceptive trials were evident, it was demonstrated that immunocontraception caused no prolonged behavioral, social, or spatial changes over the 11-yr study period. Individually identified elephants were monitored from 1999 to 2011. Minimal, short-term social disruption, with temporary changes to the herds' core ranges, was observed during the annual treatment events, particularly in the first three treatment years, when vaccinations were conducted exclusively from the ground. Thereafter, when vaccinations were conducted aerially, minor disruptions were confined to the morning of administration only. Despite sustained treatments resulting in demographic changes of fewer calves being born, treatments did not alter spatial range use, and no adverse interherd-intraherd relations were observed. Similarly, resource requirements did not change as calving still occurred, although in fewer numbers. It was concluded that PZP immunocontraception has no detectable behavioral or social consequences in elephants over the course of 11 yr, providing a convincing argument for the use of sustained immunocontraception in the medium to long term as an important tool for elephant management. Behavioral consequences of alternative management approaches should all receive similar scrutiny to enable managers to make informed decisions when weighing management interventions. PMID:24437086

  12. Liquid semen storage in elephants (Elephas maximus and Loxodonta africana): species differences and storage optimization.

    PubMed

    Kiso, Wendy K; Brown, Janine L; Siewerdt, Frank; Schmitt, Dennis L; Olson, Deborah; Crichton, Elizabeth G; Pukazhenthi, Budhan S

    2011-01-01

    Artificial insemination plays a key role in the genetic management of elephants in zoos. Because freshly extended semen is typically used for artificial insemination in elephants, it has become imperative to optimize conditions for liquid storage and semen transport. The objectives of this study were to examine the interactions between different extenders and storage temperatures on sperm total motility, progressive motility, and acrosomal integrity in Asian (Elephas maximus) and African (Loxodonta africana) elephants. Ejaculates were collected by rectal massage, diluted using a split-sample technique in 5 semen extenders: TL-Hepes (HEP), Modena (MOD), Biladyl (BIL), TEST refrigeration medium (TES), and INRA96 (INR), maintained at 35°C, 22°C, or 4°C. At 0, 4, 6, 12, and 24 hours, aliquots were removed and assessed for sperm total motility, progressive motility, and acrosomal integrity. After 24 hours of storage, African elephant spermatozoa exhibited greater longevity and higher values in sperm quality parameters compared with those of Asian elephants. In both species, semen storage at 35°C resulted in a sharp decline in all sperm quality parameters after 4 hours of storage, whereas storage at 22°C and 4°C facilitated sperm survival. In Asian elephants, MOD and HEP were most detrimental, whereas BIL, TES, and INR maintained motility up to 12 hours when spermatozoa were cooled to 22°Cor4°C. In African elephants, there were no differences among extenders. All media maintained good sperm quality parameters at 22°C or 4°C. However, although MOD, BIL, and INR were most effective at lower temperatures, HEP and TES maintained sperm motility at all storage temperatures. This study demonstrated sperm sensitivity to components of various semen extenders and storage temperatures and offers recommendations for semen extender choices for liquid semen storage for both Asian and African elephants. PMID:21127305

  13. Evaluation of Prosopis africana Seed Gum as an Extended Release Polymer for Tablet Formulation.

    PubMed

    Nadaf, Sameer; Nnamani, Petra; Jadhav, Namdeo

    2015-06-01

    In the present work, an attempt has been made to screen Prosopis africana seed gum (PG), anionic polymer for extended release tablet formulation. Different categories of drugs (charge basis) like diclofenac sodium (DS), chlorpheniramine maleate (CPM), and ibuprofen (IB) were compacted with PG and compared with different polymers (charge basis) like xanthan gum (XG), hydroxypropyl methyl cellulose (HPMC-K100M), and chitosan (CP). For each drug, 12 batches of tablets were prepared by wet granulation technique, and granules were evaluated for flow properties, compressibility, and compactibility by Heckel and Leuenberger analysis, swelling index, in vitro dissolution studies, etc. It has been observed that granules of all batches showed acceptable flowability. According to Heckel and Leuenberger analysis, granules of PG-containing compacts showed similar and satisfactory compressibility and compactibility compared to granules of other polymers. PG showed significant swelling (P?

  14. Molecular characterization of adipose tissue in the African elephant (Loxodonta africana).

    PubMed

    Nilsson, Emeli M; Fainberg, Hernan P; Choong, Siew S; Giles, Thomas C; Sells, James; May, Sean; Stansfield, Fiona J; Allen, William R; Emes, Richard D; Mostyn, Alison; Mongan, Nigel P; Yon, Lisa

    2014-01-01

    Adipose tissue (AT) is a dynamic and flexible organ with regulatory roles in physiological functions including metabolism, reproduction and inflammation; secreted adipokines, including leptin, and fatty acids facilitate many of these roles. The African elephant (Loxodonta africana) is experiencing serious challenges to optimal reproduction in captivity. The physiological and molecular basis of this impaired fertility remains unknown. AT production of leptin is a crucial molecular link between nutritional status, adiposity and fertility in many species. We propose that leptin has a similar function in the African elephant. African elephant visceral and subcutaneous adipose tissue (AT) was obtained from both sexes and a range of ages including females with known pregnancy status. RNA was extracted and histological sections created and analyzed by microarray, PCR and immunohistochemistry respectively. Gas-chromatography was used to determine the fatty acid composition of AT. Microarray expression profiling was used to compare gene expression profiles of AT from pre-pubertal versus reproductively competent adult African elephants. This study demonstrates, for the first time, leptin mRNA and protein expression in African elephant AT. The derived protein sequence of the elephant leptin protein was exploited to determine its relationship within the class I helical cytokine superfamily, which indicates that elephant leptin is most closely related to the leptin orthologs of Oryctolagus cuniculus (European rabbit), Lepus oiostolus (woolly hare), and members of the Ochotonidae (Pika). Immunohistological analysis identified considerable leptin staining within the cytoplasm of adipocytes. Significant differences in fatty acid profiles between pregnant and non-pregnant animals were revealed, most notably a reduction in both linoleic and ? linoleic acid in pregnant animals. This report forms the basis for future studies to address the effect of nutrient composition and body condition on reproduction in captive and wild elephants. PMID:24633017

  15. SPECIALISED USE OF WORKING MEMORY BY PORTIA AFRICANA, A SPIDER-EATING SALTICID

    PubMed Central

    Jackson, Robert R.

    2013-01-01

    Using expectancy-violation methods, we investigated the role of working memory in the predatory strategy of Portia africana, a salticid spider from Kenya that preys by preference on other spiders. One of this predator’s tactics is to launch opportunistic leaping attacks on to other spiders in their webs. Focussing on this particular tactic, our experiments began with a test spider on a ramp facing a lure (dead prey-spider mounted on a cork disc) that could be reached by leaping. After the test spider faced the lure for 30 s, we blocked the test spider’s view of the lure by lowering an opaque shutter before the spider leapt. When the shutter was raised 90 s later, either the same lure came into view again (control) or a different lure came into view (experimental: different prey type in same orientation or same prey type in different orientation). We recorded attack frequency (number of test spiders that leapt at the lure) and attack latency (time elapsing between shutter being raised and spiders initiating a leap). Attack latencies in control trials were not significantly different from attack latencies in experimental trials, regardless of whether it was prey type or prey orientation that changed in the experimental trials. However, compared with test spiders in the no-change control trials, significantly fewer test spiders leapt when prey type changed. There was no significant effect on attack frequency when prey orientation changed. These findings suggest that this predator represents prey type independently of prey orientation. PMID:23982622

  16. Molecular Characterization of Adipose Tissue in the African Elephant (Loxodonta africana)

    PubMed Central

    Choong, Siew S.; Giles, Thomas C.; Sells, James; May, Sean; Stansfield, Fiona J.; Allen, William R.; Emes, Richard D.; Mostyn, Alison; Mongan, Nigel P.; Yon, Lisa

    2014-01-01

    Adipose tissue (AT) is a dynamic and flexible organ with regulatory roles in physiological functions including metabolism, reproduction and inflammation; secreted adipokines, including leptin, and fatty acids facilitate many of these roles. The African elephant (Loxodonta africana) is experiencing serious challenges to optimal reproduction in captivity. The physiological and molecular basis of this impaired fertility remains unknown. AT production of leptin is a crucial molecular link between nutritional status, adiposity and fertility in many species. We propose that leptin has a similar function in the African elephant. African elephant visceral and subcutaneous adipose tissue (AT) was obtained from both sexes and a range of ages including females with known pregnancy status. RNA was extracted and histological sections created and analyzed by microarray, PCR and immunohistochemistry respectively. Gas-chromatography was used to determine the fatty acid composition of AT. Microarray expression profiling was used to compare gene expression profiles of AT from pre-pubertal versus reproductively competent adult African elephants. This study demonstrates, for the first time, leptin mRNA and protein expression in African elephant AT. The derived protein sequence of the elephant leptin protein was exploited to determine its relationship within the class I helical cytokine superfamily, which indicates that elephant leptin is most closely related to the leptin orthologs of Oryctolagus cuniculus (European rabbit), Lepus oiostolus (woolly hare), and members of the Ochotonidae (Pika). Immunohistological analysis identified considerable leptin staining within the cytoplasm of adipocytes. Significant differences in fatty acid profiles between pregnant and non-pregnant animals were revealed, most notably a reduction in both linoleic and ? linoleic acid in pregnant animals. This report forms the basis for future studies to address the effect of nutrient composition and body condition on reproduction in captive and wild elephants. PMID:24633017

  17. Video Encryption Based on OpenCV

    Microsoft Academic Search

    Cunyan Meng; Xinghui Zhang

    2010-01-01

    This paper uses the open source computer vision library (OpenCV for short) as basic library and calls library functions to achieve real-time video capture and output. We narrate the process of making the matrix interpretable as an image through analyzing the data structure of IplImage and meaning of its member variable. The library functions to read video take the video

  18. Afritoxinones A and B, dihydrofuropyran-2-ones produced by Diplodia africana the causal agent of branch dieback on Juniperus phoenicea.

    PubMed

    Evidente, Antonio; Masi, Marco; Linaldeddu, Benedetto T; Franceschini, Antonio; Scanu, Bruno; Cimmino, Alessio; Andolfi, Anna; Motta, Andrea; Maddau, Lucia

    2012-05-01

    Two phytotoxic dihydrofuropyran-2-ones, named afritoxinones A and B, were isolated from liquid culture of Diplodia africana, a fungal pathogen responsible for branch dieback of Phoenicean juniper in Italy. Additionally, six others known metabolites were isolated and characterized: oxysporone, sphaeropsidin A, epi-sphaeropsidone, R-(-)-mellein, (3R,4R)-4-hydroxymellein and (3R,4S)-4-hydroxymellein. The structures of afritoxinones A and B were established by spectroscopic and optical methods and determined to be as (3aS(*),6R(*),7aS)-6-methoxy-3a,7a-dihydro-3H,6H-furo[2,3-b]pyran-2-one and (3aR(*),6R(*),7aS)-6-methoxy-3a,7a-dihydro-3H,6H-furo[2,3-b]pyran-2-one, respectively. The phytotoxic activity of afritoxinones A and B and oxysporone was evaluated on host (Phoenicean juniper) and non-host plant (holm oak, cork oak and tomato) by cutting and leaf puncture assay. Oxysporone proved to be the most phytotoxic compound. This study represents the first report of secondary metabolites produced by D. africana. In addition, the taxonomic implications of secondary metabolites in Botryosphaeriaceae family studies are discussed. PMID:22326509

  19. Potential infection of Claviceps africana Frederickson, Mantle and de Milliano in sorghum [Sorghum bicolor (L.) Moench.] in Mexico and the United States of America

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Experiments were at College Station, USA, and Rio Bravo and Celaya, Mexico, under irrigated conditions during 2002 and 2003. Six sorghum hybrids and three male-sterile lines were planted at each location. Claviceps africana isolates were applied every other morning. Panicles were inoculated using...

  20. Disease severity and susceptibility of sorghum [Sorghum bicolor (L.)] to infection by Claviceps africana Frederickson, Mantle and de Milliano in Mexico and the United States of America

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Experiments were at College Station, USA, and Rio Bravo and Celaya, Mexico, under irrigated conditions during 2002 and 2003. Six sorghum hybrids and three male-sterile lines were planted at each location. Claviceps africana isolates were applied every other morning. Panicles were inoculated using...

  1. THE CASE OF PRUNUS AFRICANA (J.D. HOOK) KALKMAN—LESSONS FROM A NON-TIMBER FOREST PRODUCT FROM MOUNT CAMEROON

    Microsoft Academic Search

    J.-M. SCHRÖDER

    2000-01-01

    Prunus africana trees' bark from moist forests on Mt. Cameroon produce a medicinal extractive that is traded internationally with high economic significance. Because sustainable bark harvesting was widely abandoned since the early 1990s and because the economic status of the rural population has deteriorated in the area, illegal and destructive debarking became common. Under the present form of exploitation this

  2. The sexually active states of free-ranging male African elephants (Loxodonta africana): defining musth and non-musth using endocrinology physical signals and behavior

    Microsoft Academic Search

    Andre Ganswindt; Henrik B. Rasmussen; Michael Heistermann; J. Keith Hodges

    Abstract Musth in male African elephants, Loxodonta africana, is associated with increased aggressive behavior, continuous discharge of urine, copious secretions from the swollen temporal glands, and elevated androgen levels. During musth, bulls actively seek out and are preferred by estrous females although sexual activity is not restricted to the musth condition. The present study combines,recently established methods of fecal hormone,analysis

  3. In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana

    PubMed Central

    Akanni, Olubukola Oyebimpe; Owumi, Solomon Eduviere; Adaramoye, Oluwatosin Adekunle

    2014-01-01

    Objective To justify the use of Artocarpus altilis (A. altilis), Ficus exasperata (F. exasperata) and Kigelia africana (K. africana) in ethnomedicine for the treatment of several ailments and to evaluate the in vitro antioxidant, radical scavenging and arginase inhibitory potentials of these herbs and compared with catechin (Standard). Methods Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide (H2O2) and hydroxyl (OH) radicals scavenging methods. The flavonoids and phenolics content, inhibition of arginase activity, Fe2+/ascorbate-induced lipid peroxidation (LPO) and reducing power were also determined. Results The A. altilis, F. exasperata and K. africana showed dose-dependent and significant scavenging of DPPH, H2O2 and OH radicals in vitro relative to catechin. The A. altilis and F. exasperata effectively scavenged DPPH radical with IC50 of 593 and 635 µg/mL and, OH radical with IC50 of 487 and 514 µg/mL, respectively. The DPPH and OH radicals scavenging activities followed the order A. altilis>F. exasperata>K. africana. In addition, A. altilis and F. exasperata significantly (P<0.05) inhibited LPO in a dose-dependent manner. The A. altilis extract had the most potent inhibitory activity against LPO with 79% relative to catechin (28%) at 750 µg/mL. The reducing power followed the order: A. altilis>Catechin>F. exasperata>K. africana at 1?000 µg/mL. The A. altilis at 500 and 750 µg/mL significantly (P<0.05) inhibited arginase activity by 63% and 67%, respectively. The flavonoids contents were found to be highest in A. altilis. Conclusions Extracts of A. altilis and F. exasperata are potent antioxidative agents with strong radical scavenging activity and inhibition of lipid peroxidation. PMID:25183137

  4. Ch OpenCV for interactive open architecture computer vision

    Microsoft Academic Search

    Qingcang Yu; Harry H. Cheng; Wayne W. Cheng; Xiaodong Zhou

    2004-01-01

    In this paper, design and implementation of an interactive open architecture computer vision software package called Ch OpenCV is presented. Benefiting from both Ch and OpenCV, Ch OpenCV has many salient features. It is interactive, capable of interface with binary static or dynamical C\\/C++ libraries, integrated with advanced numerical features and embeddable. It is especially suitable for rapid prototyping, web-based

  5. Divergent pattern of nuclear genetic diversity across the range of the Afromontane Prunus africana mirrors variable climate of African highlands

    PubMed Central

    Kadu, Caroline A. C.; Konrad, Heino; Schueler, Silvio; Muluvi, Geoffrey M.; Eyog-Matig, Oscar; Muchugi, Alice; Williams, Vivienne L.; Ramamonjisoa, Lolona; Kapinga, Consolatha; Foahom, Bernard; Katsvanga, Cuthbert; Hafashimana, David; Obama, Crisantos; Geburek, Thomas

    2013-01-01

    Background and Aims Afromontane forest ecosystems share a high similarity of plant and animal biodiversity, although they occur mainly on isolated mountain massifs throughout the continent. This resemblance has long provoked questions on former wider distribution of Afromontane forests. In this study Prunus africana (one of the character trees of Afromontane forests) is used as a model for understanding the biogeography of this vegetation zone. Methods Thirty natural populations from nine African countries covering a large part of Afromontane regions were analysed using six nuclear microsatellites. Standard population genetic analysis as well as Bayesian and maximum likelihood models were used to infer genetic diversity, population differentiation, barriers to gene flow, and recent and all migration among populations. Key Results Prunus africana exhibits strong divergence among five main Afromontane regions: West Africa, East Africa west of the Eastern Rift Valley (ERV), East Africa east of the ERV, southern Africa and Madagascar. The strongest divergence was evident between Madagascar and continental Africa. Populations from West Africa showed high similarity with East African populations west of the ERV, whereas populations east of the ERV are closely related to populations of southern Africa, respectively. Conclusions The observed patterns indicate divergent population history across the continent most likely associated to Pleistocene changes in climatic conditions. The high genetic similarity between populations of West Africa with population of East Africa west of the ERV is in agreement with faunistic and floristic patterns and provides further evidence for a historical migration route. Contrasting estimates of recent and historical gene flow indicate a shift of the main barrier to gene flow from the Lake Victoria basin to the ERV, highlighting the dynamic environmental and evolutionary history of the region. PMID:23250908

  6. SeeingWithOPenCV A Computer-VisionLibrary

    E-print Network

    Oh, Paul

    SeeingWithOPenCV A Computer-VisionLibrary ,lPenCV - Intel'sfree,oPen-source t lcomputerCVinterfaces. .!r OpenCVOverview OpenCVis a free, oPen-source computervision libraryfor C/C++ programmers.You can,facetrack- ing,facerecognition,Kalmanfiltering, anda varietyof artificial-intelligence(Al) methods- inready

  7. Rachel E. Pepper CV 1 of 4 Rachel E. Pepper

    E-print Network

    Koehl, Mimi

    Rachel E. Pepper ­ CV ­ 1 of 4 Rachel E. Pepper University of California at Berkeley, 1005 Valley://spot.colorado.edu/~pepperr · e-mail: rachel.pepper@berkeley.edu EDUCATION Harvard University (Cambridge, MA) Ph.D., Physics University, 2005-2009 Graduate Researcher Advisor: Howard A. Stone #12;Rachel E. Pepper ­ CV ­ 2 of 4 Thesis

  8. Properties of NAC and CV[NAC] for Energy Models 

    E-print Network

    Ruch, D. K.

    1993-01-01

    . If these conditions do hold, then the NAC estimate will be more reliable than the intercept estimate. Theorem 2.1. Suppose that the model E = a + bT is fit to the data set {Tj, Ei}. Then \\CV(d)\\ >\\CV{NAC)\\ if Proof. Using the standard definition of variance (see any...

  9. BRIEF C.V. DR JOHN C.V. ("JACK") PEZZEY, as at 14 August 2014 http://people.anu.edu.au/jack.pezzey EDUCATION AND EMPLOYMENT

    E-print Network

    Pezzey, Jack

    BRIEF C.V. DR JOHN C.V. ("JACK") PEZZEY, as at 14 August 2014 http citations, H-index = 25) Pezzey, John C.V. and Paul J. Burke (2014). "Towards a more inclusive and precautionary indicator of global sustainability." Ecological Economics, 106, 141-154. Pezzey, John C.V. (2014

  10. Characterization of a protein tyrosine phosphatase gene CvBV202 from Cotesia vestalis polydnavirus (CvBV).

    PubMed

    Shi, Min; Chen, Ya-Feng; Yao, Yuan; Huang, Fang; Chen, Xue-Xin

    2008-06-01

    Cotesia vestalis (Haliday) (Hymenoptera: Braconidae) is an endoparasitoid of the larval stage of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) and injects a polydnavirus (CvBV) into its host during oviposition. In this paper we characterize CvBV202 and its product. CvBV202 is located on segment S2 of CvBV genome; it has a size of 984 bp and encodes a putative protein of 328 amino acids, including protein phosphatase domain and tyrosine-specific protein phosphatase domain. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected for 6 days. Tissue-specific patterns of this gene expression showed that CvBV202 had a close relationship with the host's physiological alternations including immunosuppression, modulation of hormone titer, and nutrition metabolism. The protein was detected in the parasitized hosts at 12 h p.p. using western blot assay. The product of CvBV202 was found to be around 59 kDa, much larger than the predicted molecular weight of 37.8 kDa, suggesting that post-translational modification of CvBV202 occurs in host cells, corresponding with the existence of many post-translational modification sites. Immunofluorescence staining and confocal laser scanning microscopy revealed that CvBV202 and the fused protein eGFP-CvBV202 were observed both in the nuclear region and cytoplasm of the hemocytes of the naturally parasitized host larvae and rBac-eGFP-CvBV202-infected Tn-5B1-4 cells, respectively. PMID:18425572

  11. Cactodera eremica n. sp., Afenestrata africana (Luc et al., 1973) n. gen., n. comb., and an Emended Diagnosis of Sarisodera Wouts and Sher, 1971 (Heteroderidae)

    PubMed Central

    Baldwin, J. G.; Bell, A. H.

    1985-01-01

    Systematic contributions to Heteroderidae include description of Cactodera eremica n. sp., an emended diagnosis of Sarisodera Wouts and Sher, 1971, and proposal of a new genus and new combination, Afenestrata africana (synonym Sarisodera africana Luc et al., 1973). Cactodera eremica, from the roots of shadscale in Utah, most closely resembles Cactodera thornei (Golden and Raski, 1977) but differs by the presence of a finely striated cuticle, a fine surface pattern on eggs, a shorter female stylet, distance of the DGO from the stylet, vulval slit, and smaller diameter of circumfenestra, as well as a shorter tail in second-stage juveniles. The response of the host to C. eremica is similar to other Heterodera sensu lato including a large syncytium with wall ingrowths. The diagnosis of Sarisodera is emended to exclude cysts, which do not form in the type species, S. hydrophila. Afenestrata africana differs from S. hydrophila by the formation of cysts, the dorsal position of the anus in females, the shorter stylet, and a pore-like phasmid opening in second-stage juveniles. In addition, the lip pattern of males and juveniles is characterized by a greater degree of fusion of lip parts, the host response is a syncytium (versus a single uninucleate giant cell in S. hydrophila), and the cuticle is thinner and lacks a D layer. Unlike Heterodera, the cyst of Afenestrata lacks fenestrae. PMID:19294081

  12. ES4 NPP-FM5 Ed1-CV

    Atmospheric Science Data Center

    2015-06-17

    ... Data Products Catalog:  DPC ES4 R7V1  (PDF) Validation Graphics Readme Files:  Readme R6V1-894 ... Data:  Note:  Edition1-CV is for instrument validation purposes only and not suited for science publications. ...

  13. SeeingWithOPenCV FindingFacesin lmages

    E-print Network

    Oh, Paul

    severalscales. The classifierusesdata storedin an XMLfileto decidehow to classify each image location.The OPenCVClassifier,Anyhow?"explains want it to use.The one l'll be usingiscalledhaarcascade-frontal face-default.xml.In OPenCV version1Pn'icv-noor]is the Path to your OpenCVinstallation.For example,if you'reonWindowsXP FIGURE1.Facedetectionwith OpenCV

  14. Ch OpenCV for interactive open architecture computer vision Qingcang Yua

    E-print Network

    Cheng, Harry H.

    Ch OpenCV for interactive open architecture computer vision Qingcang Yua , Harry H. Chenga,*, Wayne and implementation of an interactive open architecture computer vision software package called Ch OpenCV is presented. Benefiting from both Ch and OpenCV, Ch OpenCV has many salient features. It is interactive, capable

  15. Barium sulphate in a Saharan CV chondrite

    NASA Astrophysics Data System (ADS)

    Ash, R. D.

    1994-07-01

    Acfer 086 (CV3) was recovered from the Sahara in 1989. A thin section was studied for weathering products. During routine microprobe analysis a phase was found containing Ba and S in approximate 1:1 atomic ratio. The only other element detected at more than trace level was Fe (less than 2%), possibly from surrounding Fe-rich phases. It is concluded that the only likely mineral with this chemistry is barytes, BaSO4. The barytes occurs as isolated, 1-10-micron, irregularly shaped grains. It is present exclusively in inclusions, both metal/C-rich and chondrules and chondrule fragments. None were observed in the matrix. At its highest the barytes density reached greater than 50 grains in an area measuring 300 x 200 microns. At present, whether the barytes is terrestrial or meteoritic remains unclear. Reasons for both cases are given and discussed. While it is an intriguing possibility that the barytes in Acfer 086 may be a primary meteoritic mineral, and considerable evidence would support this view, it must be treated with great caution. Although texturally it is unlike a secondary phase and there are difficulties with the introduction of barytes by groundwater, this cannot be dismissed. The high and variable Ba contents of the Acfer/El Djouf Saharan CR chondrites are strong evidence for the formation of secondary barytes during residence on the desert floor. If terrestrial, the presence and distribution pattern of barytes in Acfer 086 has potentially important consequences for chemical and isotopic analyses of many elements in both bulk and inclusions of meteorite finds from the deserts of the world.

  16. Activation and inhibition of thermosensitive TRP channels by voacangine, an alkaloid present in Voacanga africana, an African tree.

    PubMed

    Terada, Yuko; Horie, Syunji; Takayama, Hiromitsu; Uchida, Kunitoshi; Tominaga, Makoto; Watanabe, Tatsuo

    2014-02-28

    Voacangine (1) is an alkaloid found in the root bark of Voacanga africana. Our previous work has suggested that 1 is a novel transient receptor potential vanilloid type 1 (TRPV1) antagonist. In this study, the agonist and antagonist activities of 1 were examined against thermosensitive TRP channels. Channel activity was evaluated mainly using TRP channel-expressing HEK cells and calcium imaging. Herein, it was shown that 1 acts as an antagonist for TRPV1 and TRPM8 but as an agonist for TRPA1 (EC50, 8 ?M). The compound competitively blocked capsaicin binding to TRPV1 (IC50, 50 ?M). Voacangine (1) competitively inhibited the binding of menthol to TRPM8 (IC50, 9 ?M), but it showed noncompetitive inhibition against icilin (IC50, 7 ?M). Moreover, the compound selectively abrogated chemical agonist-induced TRPM8 activation and did not affect cold-induced activation. Among these effects, the TRPM8 inhibition profile is unique and noteworthy, because to date no studies have reported a menthol competitive inhibitor of TRPM8 derived from a natural source. Furthermore, this is the first report of a stimulus-selective TRPM8 antagonist. Accordingly, 1 may contribute to the development of a novel class of stimulus-selective TRPM8 blockers. PMID:24484240

  17. Phoretic interaction between the kangaroo leech Marsupiobdella africana (Hirudinea: Glossiphoniidae) and the cape river crab Potamonautes perlatus (Decapoda: Potamonautidae)?

    PubMed Central

    Badets, Mathieu; Preez, Louis Du

    2013-01-01

    The South African leech Marsupiobdella africana is a temporary ectoparasite of the amphibian Xenopus laevis, has a phoretic association with a freshwater crab Potamonautes perlatus, and exhibits advanced parental care by incubating its offspring in a brood pouch. Because phoretic associations are usually regarded to favor the phoront’s dispersion, its occurrence within the biology of a parasitic species reflects an intimate context of interactions. In addition to phoresy, attachment to the crab may confer other advantages pertaining to offspring development and predator avoidance, dispersion and the parasitic life cycle. Two ponds where amphibian and crab hosts co-occur were sampled twice a month for a period of 1 year. The population dynamics of the leeches and their use of specific microhabitats as attachment sites on the crabs were also investigated. Results indicate a direct relationship between intra-specific variation in the sex ratio among captured crab hosts and the number of leeches recruited over time. The attachments to specific microhabitats on the hard surfaces of the host suggest a proximal proximate anti-predatory strategy. Finally, the importance of oxygen accessibility for the offspring development has been investigated experimentally. Results revealed a remarkable network of interactions linking all partners of this system raising the question as to whether the crabs merely act as a vehicle or play a role within the parasitic life cycle. PMID:24918071

  18. Evaluation of antitranspirants on creeping bentgrass (Agrostis palustris Huds., cv. 'Penncros') and bermudagrass (Cynodon dactylon (L.) Pers. X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway')

    E-print Network

    Stahnke, Gwen Kristine

    1981-01-01

    bentgrass (Agrostis palustris Huds. , cv. 'Penncross') and bermudagrass (Cynodon dactylon (L. ) Pers. X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway'), two turfgrasses commonly used as sports turfs, were used to evaluate antitranspirants in this study...

  19. The Social and Ecological Integration of Captive-Raised Adolescent Male African Elephants (Loxodonta africana) into a Wild Population

    PubMed Central

    Evans, Kate; Moore, Randall; Harris, Stephen

    2013-01-01

    Background A rapid rise in the number of captive African elephants (Loxodonta africana) used in the tourism industry in southern Africa and orphaned elephants in human care has led to concerns about their long-term management, particularly males. One solution is to release them into the wild at adolescence, when young males naturally leave their herd. However, this raises significant welfare concerns: little is known about how well released elephants integrate into wild populations and whether they pose a greater threat to humans than wild elephants. We document the release of three captive-raised adolescent male African elephants in the Okavango Delta, Botswana. Methodology/Principal Findings Despite having been part of a herd of working elephants for at least eight years, the three males progressively integrated into the complex fission-fusion society of wild bull elephants. In the three years following release, they showed no tendency to be closer to human habitation, and there were no significant differences between wild and captive-raised adolescent males in the total number of social interactions, size of ranges and habitat use. However, the captive-raised elephants sparred less and vocalised more, and spent more time alone and in smaller social groups. Thereafter the released elephants continued to expand their ranges and interact with both mixed-sex herds and males. One male was shot by farmers 94 months after release, along with ten wild elephants, on a ranch outside the protected area. Conclusions/Significance We show that captive-raised adolescent male elephants can integrate into a wild population. Long-term studies are required to determine the longevity, breeding success, and eventual fate of released male elephants, but we identified no significant short-term welfare problems for the released elephants or recipient population. Release of captive-raised mammals with complex social systems is a husbandry option that should be explored further. PMID:23437076

  20. Properties of NAC and CV[NAC] for Energy Models

    E-print Network

    Ruch, D. K.

    1993-01-01

    . If these conditions do hold, then the NAC estimate will be more reliable than the intercept estimate. Theorem 2.1. Suppose that the model E = a + bT is fit to the data set {Tj, Ei}. Then \\CV(d)\\ >\\CV{NAC)\\ if Proof. Using the standard definition of variance (see any... the energy use is zero. Thus for most data sets used to model energy use, \\T^ ? TQ\\ will be quite large in comparison to TL ? T . The condition " \\TL ? To\\ > \\/2- sy" is not a strong assumption, for this means that the spread of the data, as measured by sT, a...

  1. Endothelium/Nitric Oxide Mediates the Vasorelaxant and Antihypertensive Effects of the Aqueous Extract from the Stem Bark of Mammea africana Sabine (Guttiferae)

    PubMed Central

    Nguelefack-Mbuyo, Elvine Pami; Dongmo, Alain Bertrand; Nguelefack, Télesphore Benoît; Kamanyi, Albert; Kamtchouing, Pierre; Dimo, Théophile

    2012-01-01

    This study evaluates the vasorelaxant and antihypertensive effects of the aqueous extract from the stem bark of M. africana (AEMA). AEMA was tested in vitro on intact or endothelium-denuded rats' aorta rings precontracted with KCl or norepinephrine in absence or in presence of L-NAME or glibenclamide. The effect of a single concentration (300??g/mL) of AEMA was also examined on the concentration-response curve of KCl. In vivo, the antihypertensive effects of AEMA (200?mg/kg/day) were evaluated in male Wistar rats treated with L-NAME (40?mg/kg/day) for 4 weeks. AEMA relaxed aorta rings precontracted with NE or KCl with respective EC50 values of 0.36??g/mL and 197.60??g/mL. The destruction of endothelium or pretreatment of aorta rings with L-NAME shifted the EC50 of AEMA from 0.36??g/mL to 40.65??g/mL and 20.20??g/mL, respectively. The vasorelaxant activity of M. africana was significantly inhibited in presence of glibenclamide. AEMA also significantly inhibited the concentration-response curve of KCl. Administered orally, AEMA induced acute and chronic antihypertensive effects and normalized renal NO level. These results show that the vasorelaxant activity of AEMA might be mediated by the activation of the NO-cGMP-ATP-dependent potassium channels pathway and might predominantly account for its antihypertensive effect. PMID:23008745

  2. Rachel E. Pepper CV 1 of 5 Rachel E. Pepper

    E-print Network

    Koehl, Mimi

    Rachel E. Pepper ­ CV ­ 1 of 5 Rachel E. Pepper University of California Berkeley, 1005 Valley Life Sciences Bldg #3140, Berkeley CA 94720-3140 cell: 617-272-5914 · web: ib.berkeley.edu/labs/koehl/pepper/pepper.html · e-mail: rachel.pepper@berkeley.edu EDUCATION Harvard University (Cambridge, MA) Ph.D., Physics

  3. Rachel E. Pepper CV 1 of 5 Rachel E. Pepper

    E-print Network

    Koehl, Mimi

    Rachel E. Pepper ­ CV ­ 1 of 5 Rachel E. Pepper University of California at Berkeley, 1005 Valley Life Sciences Bldg #3140, Berkeley CA 94720-3140 cell: 617-272-5914 · web: ib.berkeley.edu/labs/koehl/pepper/pepper.html · e-mail: rachel.pepper@berkeley.edu EDUCATION Harvard University (Cambridge, MA) Ph.D., Physics

  4. FRIABLE AND COMPACT CALLUS OF LILIUM LONGIFLORUM CV. NELLIE WHITE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two types of callus, a fast-growing friable callus and a slow-growing compact callus, were initiated from bulb scales of Lilium longiflorum cv. Nellie White. Sucrose affected the induction of friable callus. Friable callus was initiated from bulb scales cultured on Murashige and Skoog’s basal salt...

  5. The Implementation of Lane Detective Based on OpenCV

    Microsoft Academic Search

    Wu Ye; Shan Yuetian; Xu Yunhe; Wang Shu; Zhuang Yuchen

    2010-01-01

    In intelligent vehicle systems, lane detection is one of the most important parts. This paper presents a lane detection algorithm that based on Hough transform. Principle of the algorithm and the implementation base on OpenCV are discussed in detail. The algorithm was verified at the end of this paper.

  6. Visitor face tracking system using OpenCV library

    Microsoft Academic Search

    Yong Kok Ching; A. S. Prabuwono; R. Sulaiman

    2009-01-01

    This paper presents the development of visitor face tracking system using OpenCV library. This system can be used for security purpose to record the visitor face. The user can identify who had been visiting him\\/her when away from the place. Visitors just need to appear in front of the web camera for at least 10 second, their image will be

  7. CV Marianne Espeland Marianne Espeland, PhD

    E-print Network

    Espeland, Marianne

    CV Marianne Espeland Marianne Espeland, PhD Museum of Comparative Zoology, Room 401c, Harvard@fas.harvard.edu Current position Harvard University. Museum of Comparative Zoology and Department of Organismic and Evolutionary biology. Postdoctoral fellow Education Oct. 2010 Doctor of Philosophy (PhD) in systematic zoology

  8. Morphological analyses of spring wheat (CIMMYT cv. PCYT-10) somaclones

    Microsoft Academic Search

    Z. N. Hashim; W. F. Campbell; J. G. Carman

    1990-01-01

    The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige & Skoog's inorganic salts. Embryos cultured

  9. Progressive alteration in CV3 chondrites: More evidence for asteroidal alteration

    Microsoft Academic Search

    Alexander N. Krot; Michael I. Petaev; Edward R. D. Scott; Byeon-Gak Choi; Michael E. Zolensky; Klaus Keil

    1998-01-01

    The oxidized CV3 chondrites can be divided into two major subgroups or lithologies, Bali-like (CV3oxB) and Allende-like (CV3oxA), in which chondrules, CAIs and matrices show characteristic alteration features (Weisberg et al., 1997; Krot et al., 1997d; Kimura and Ikeda, 1997). The CV3oxB lithology is present in Bali, Kaba, parts of the Mokoia breccia and, possibly, in Grosnaja and ALH85006. It

  10. GpuCV: an opensource GPU-accelerated framework forimage processing and computer vision

    Microsoft Academic Search

    Yannick Allusse; Patrick Horain; Ankit Agarwal; Cindula Saipriyadarshan

    2008-01-01

    This paper presents GpuCV, an open source multi-platform library for easily developing GPU-accelerated image processing and Computer Vision operators and applications. It is meant for computer vision scientist not familiar with GPU technologies. It is designed to be compatible with Intel's OpenCV library by offering GPU-accelerated operators that can be integrated into native OpenCV applications. The GpuCV framework transparently manages

  11. An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection

    E-print Network

    Rose, Jonathan

    An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection for the Acceleration of OpenCV Object Detection Braiden Brousseau Master of Applied Science Graduate Department . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.4 OpenCV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.5 Multi

  12. An Intensive HST/COS Study of 31 CV WDs

    NASA Astrophysics Data System (ADS)

    Pala, A. F.; Gänsicke, B. T.; Beuermann, K.; Bildsten, L.; De Martino, D.; Godon, P.; Henden, A. A.; Hubeny, I.; Knigge, C.; Long, K. S.; Marsh, T. R.; Patterson, J.; Schreiber, M. R.; Sion, E. M.; Szkody, P.; Townsley, D.; Zorotovic, M.

    2015-06-01

    Accretion is the key ingredient in the evolution of white dwarfs (WDs) in cataclysmic variables (CVs), with important implications for their potential as progenitors for Type Ia Supernovae. The effective temperature (Teff) gives a direct measure of the average accretion rate and we report here the Teff measurement for 31 CV WDs, observed as a part of a 122 orbit HST program. The HST spectra alone cannot resolve the degeneracy between Teff and the surface gravity log g, and we will use phase-resolved X-Shooter observations to (i) measure the mass ratio of the CVs from the reflex motion of both the WD and the donor star (ii) to establish the spectral energy distribution (SED), which constrains both Teff and log g. Combining the X-Shooter and HST data, we can measure the WD masses to a few percent, and will be able to answer the question whether accreting CV WDs grow in mass.

  13. A camera calibration technique based on OpenCV

    Microsoft Academic Search

    Y. M. Wang; Y. Li; J. B. Zheng

    2010-01-01

    Camera calibration is very improtant in 3D computer vision systems. In this paper, the camera model in OpenCV (open source computer vision library) is discussed, the non-linear distortion of the tangential and radial distortion aberration are considered. Based on these, we introduce the corner extraction of the Camera Calibration Toolbox for Matlab, and an arithmetic of camera calibration based on

  14. Organizational Chart Africana Studies

    E-print Network

    Kidd, William S. F.

    . Neely Economics B. Daniel Art & Art History R. Dressler LACS M. Lifchitz Sociology N. Denton Atm. & Env. Sci. C. Thorncroft English R. Craig Math & Stats K. Zhu Theatre A. Lyons Biological Sciences R. Zitomer Geog. & Planning C. Lawson Music R. Hoyt W, Gen & Sex St. V. Ng Philosophy P. Magnus Communication

  15. Antimicrobial activity and probable mechanisms of action of medicinal plants of Kenya: Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus.

    PubMed

    Mwitari, Peter G; Ayeka, Peter A; Ondicho, Joyce; Matu, Esther N; Bii, Christine C

    2013-01-01

    Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus are used traditionally in Kenya for treatment of microbial infections and cancer. Information on their use is available, but scientific data on their bioactivity, safety and mechanisms of action is still scanty. A study was conducted on the effect of organic extracts of these plants on both bacterial and fungal strains, and their mechanisms of action. Extracts were evaluated through the disc diffusion assay. Bacteria and yeast test strains were cultured on Mueller-Hinton agar and on Sabouraud dextrose agar for the filamentous fungi. A 0.5 McFarland standard suspension was prepared. Sterile paper discs 6 mm in diameter impregnated with 10 µl of the test extract (100 mg/ml) were aseptically placed onto the surface of the inoculated media. Chloramphenicol (30 µg) and fluconazole (25 µg) were used as standards. Discs impregnated with dissolution medium were used as controls. Activity of the extracts was expressed according to zone of inhibition diameter. MIC was determined at 0.78-100 mg/ml. Safety studies were carried using Cell Counting Kit 8 cell proliferation assay protocol. To evaluate extracts mechanisms of action, IEC-6 cells and RT-PCR technique was employed in vitro to evaluate Interleukin 7 cytokine. Investigated plants extracts have both bactericidal and fungicidal activity. W. ugandensis is cytotoxic at IC50<50 µg/ml with MIC values of less than 0.78 mg/ml. Prunus africana shuts down expression of IL 7 mRNA at 50 µg/ml. W. somnifera has the best antimicrobial (1.5625 mg/ml), immunopotentiation (2 times IL 7 mRNA expression) and safety level (IC50>200 µg/ml). Fractions from W. ugandensis and W. somnifera too demonstrated antimicrobial activity. Mechanisms of action can largely be attributed to cytotoxicity, Gene silencing and immunopotentiation. Use of medicinal plants in traditional medicine has been justified and possible mechanisms of action demonstrated. Studies to isolate and characterize the bioactive constituents continue. PMID:23785437

  16. Antimicrobial Activity and Probable Mechanisms of Action of Medicinal Plants of Kenya: Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus

    PubMed Central

    Mwitari, Peter G.; Ayeka, Peter A.; Ondicho, Joyce; Matu, Esther N.; Bii, Christine C.

    2013-01-01

    Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus are used traditionally in Kenya for treatment of microbial infections and cancer. Information on their use is available, but scientific data on their bioactivity, safety and mechanisms of action is still scanty. A study was conducted on the effect of organic extracts of these plants on both bacterial and fungal strains, and their mechanisms of action. Extracts were evaluated through the disc diffusion assay. Bacteria and yeast test strains were cultured on Mueller-Hinton agar and on Sabouraud dextrose agar for the filamentous fungi. A 0.5 McFarland standard suspension was prepared. Sterile paper discs 6 mm in diameter impregnated with 10 µl of the test extract (100 mg/ml) were aseptically placed onto the surface of the inoculated media. Chloramphenicol (30 µg) and fluconazole (25 µg) were used as standards. Discs impregnated with dissolution medium were used as controls. Activity of the extracts was expressed according to zone of inhibition diameter. MIC was determined at 0.78–100 mg/ml. Safety studies were carried using Cell Counting Kit 8 cell proliferation assay protocol. To evaluate extracts mechanisms of action, IEC-6 cells and RT-PCR technique was employed in vitro to evaluate Interleukin 7 cytokine. Investigated plants extracts have both bactericidal and fungicidal activity. W. ugandensis is cytotoxic at IC50<50 µg/ml with MIC values of less than 0.78 mg/ml. Prunus africana shuts down expression of IL 7 mRNA at 50 µg/ml. W. somnifera has the best antimicrobial (1.5625 mg/ml), immunopotentiation (2 times IL 7 mRNA expression) and safety level (IC50>200 µg/ml). Fractions from W. ugandensis and W. somnifera too demonstrated antimicrobial activity. Mechanisms of action can largely be attributed to cytotoxicity, Gene silencing and immunopotentiation. Use of medicinal plants in traditional medicine has been justified and possible mechanisms of action demonstrated. Studies to isolate and characterize the bioactive constituents continue. PMID:23785437

  17. Sara Gran Mitchell CV, Page 1 of 6 SARA GRAN MITCHELL

    E-print Network

    Sara Gran Mitchell CV, Page 1 of 6 SARA GRAN MITCHELL Curriculum Vitae Department of Biology PO Box Gran Mitchell CV, Page 2 of 6 EXPERIENCE 2006 - present: Assistant Professor, Department of Biology technician, Minnesota Geological Survey, St. Paul, Minnesota. AWARDS AND HONORS · Howard A. Coombs Fellowship

  18. Research and improvement of face detection algorithm based on the OpenCV

    Microsoft Academic Search

    Min Zuo; Guangping Zeng; Xuyan Tu

    2010-01-01

    This article describes functions and advantages of the OpenCV library, and explains the meaning, development status, applications, and difficulties of face detection technology, analysis the idea of Adaboost classifier algorithm, and achieves the detection procedures for face using OpenCV, and proposes the improved method based on the the original algorithm.

  19. A Preliminary Investigation on Computer Vision for Telemedicine Systems Using OpenCV

    Microsoft Academic Search

    Zenon Chaczko; LaReine A. Yeoh; Venkatesh Mahadevan

    2010-01-01

    OpenCV is typically, an open source vision library suitable for computer vision programs. In this paper, we present some of our preliminary investigation experiences of developing Computer Vision programs using OpenCV for robotic telemedicine cluster system, within the practice based ICTD subject within the undergraduate Software Engineering Program at the Faculty of Engineering, University of Technology Sydney (UTS). Firstly, it

  20. Fabric defect detection based on open source computer vision library OpenCV

    Microsoft Academic Search

    Xiaojun Jia

    2010-01-01

    A method for fabric defect detection based on OpenCV with rich computer vision and image processing algorithms and functions is presented. Firstly, OpenCV image processing functions implement fabric image preprocessing. We use morphological opening and closing operations to segment image because of their blur defects. Secondly, “seed filling” algorithm is applied to connect broke lines to keep defect edge smoothing.

  1. In Situ Measurement of Oxygen Isotope Compositions of Magnetite in the Allende CV3 Chondrite

    Microsoft Academic Search

    B.-G. Choi; C. D. Coath; L. Leshin; J. Wang; K. D. McKeegan; J. T. Wasson

    1995-01-01

    Magnetite is relatively abundant in the oxidized CV chondrites. This study was undertaken to examine possible origins in nebular or in parent-body processes. Magnetite in Allende has several distinct characteristics compared to that of the other CV chondrites. Unlike Mokoia and Vigarano, magnetite in Allende is almost exclusively found in chondrules, where it typically forms spherical nodules often associated with

  2. 75 FR 54887 - Determination of Regulatory Review Period for Purposes of Patent Extension; REPEL-CV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-09

    ...FDA-2009-E-0414] Determination of Regulatory Review Period for Purposes of Patent Extension; REPEL-CV...has determined the regulatory review period for REPEL-CV and is publishing this...provide that a patent may be extended for a period of up to 5 years so long as the...

  3. NASA/ESA CV-990 airborne simulation of Spacelab

    NASA Technical Reports Server (NTRS)

    Mulholland, D.; Neel, C.; De Waard, J.; Lovelett, R.; Weaver, L.; Parker, R.

    1975-01-01

    The paper describes the joint NASA/ESA extensive Spacelab simulation using the NASA CV-990 airborne laboratory. The scientific payload was selected to conduct studies in upper atmospheric physics and infrared astronomy. Two experiment operators from Europe and two from the U.S. were selected to live aboard the aircraft along with a mission manager for a six-day period and operate the experiments in behalf of the principal scientists. The mission was successful and provided extensive data relevant to Spacelab objectives on overall management of a complex international payload; experiment preparation, testing, and integration; training for proxy operation in space; data handling; multiexperimenter use of common experimenter facilities (telescopes); and schedule requirements to prepare for such a Spacelab mission.

  4. Bioactive phthalides from Ligusticum sinense Oliv cv. Chaxiong.

    PubMed

    Wei, Qian; Yang, Jianbo; Ren, Jin; Wang, Aiguo; Ji, Tengfei; Su, Yalun

    2014-03-01

    Five new phthalides (1-4, 6), two new natural products (5, 7) and five known phthalides (8-12) were isolated from the aerial parts of Ligusticum sinense Oliv cv. Chaxiong. Their structures were elucidated by HR-ESI-MS, UV, IR, 1D and 2D NMR (HSQC, HMBC, (1)H-(1)H COSY, NOESY) methods. The absolute configurations were established by the circular dichroism (CD) spectrum and the modified Mosher's method. Compounds 1-8 were tested against SK-N-SH cell depriving oxygen and glucose and showed different degrees of increasing the cell survival, among which compounds 1, 4 and 8 (10 ?M) showed higher cell survival than Ginsenoside Rg1. PMID:24456660

  5. The sexually active states of free-ranging male African elephants (Loxodonta africana): defining musth and non-musth using endocrinology, physical signals, and behavior.

    PubMed

    Ganswindt, André; Rasmussen, Henrik B; Heistermann, Michael; Hodges, J Keith

    2005-01-01

    Musth in male African elephants, Loxodonta africana, is associated with increased aggressive behavior, continuous discharge of urine, copious secretions from the swollen temporal glands, and elevated androgen levels. During musth, bulls actively seek out and are preferred by estrous females although sexual activity is not restricted to the musth condition. The present study combines recently established methods of fecal hormone analysis with long-term observations on male-female associations as well as the presence and intensity of physical signals to provide a more detailed picture about the physical, physiological, and behavioral characteristics of different states of sexual activity in free-ranging African elephants. Based on quantitative shifts in individual bull association patterns, the presence of different physical signals, and significant differences in androgen levels, a total of three potential sub-categories for sexually active bulls could be established. The results demonstrate that elevations in androgen levels are only observed in sexually active animals showing temporal gland secretion and/or urine dribbling, but are not related to the age of the individual. Further, none of the sexually active states showed elevated glucocorticoid output indicating that musth does not represent an HPA-mediated stress condition. On the basis of these results, we suggest that the term "musth" should be exclusively used for the competitive state in sexually active male elephants and that the presence of urine dribbling should be the physical signal used for defining this state. PMID:15579269

  6. Petrofabrics in Fine-Grained Rims of Vigarano CV3 Chondrite: Insights from EBSD and AFM Studies

    NASA Astrophysics Data System (ADS)

    Soulié, C.; Ildefonse, B.; Libourel, G.

    2013-09-01

    EBSD and AFM analyses were used to characterize the petrofabric of fayalitic olivines in the matrix and chondrule fine-grained rims of Vigarano CV3 chondrite and to compare them with other CV chondrites.

  7. Cardiovascular (CV) responsivity and recovery to acute stress and future CV functioning in youth with family histories of CV disease: a 4-year longitudinal study.

    PubMed

    Treiber, F A; Musante, L; Kapuku, G; Davis, C; Litaker, M; Davis, H

    2001-05-01

    Blood pressure (BP) and heart rate (HR) data obtained during supine rest, in response to and recovery from four laboratory stressors in a baseline year were used to predict supine resting BP and HR values obtained during each of four consecutive annual follow-up evaluations. Subjects were 385 normotensive youth [95 African American (AA) males, 106 AA females, 92 European American (EA) males, 92 EA females] (mean age 12.7+/-2.6 at baseline year) with a positive family history of cardiovascular disease (CVD). During the baseline evaluation subjects were presented with four laboratory stressors (namely, postural change, video game challenge, social competence interview, and parent--child conflict discussion). The BP and HR values taken during each of the laboratory stressors and during the post stressor recovery periods were converted to z-scores which were averaged to yield aggregate measures for systolic and diastolic BP and HR responsivity and recovery. The data obtained during the baseline evaluation were subsequently used to predict the follow-up values of supine resting BP and HR. The prediction models were fairly consistent across each of the 4 follow-up years. Responsivity or recovery accounted for up to 6% of the total variance after accounting for baseline values. Within the prediction models responsivity or recovery accounted for 4--56% of the variance. The predictive value of the derived models did not decline from one annual evaluation to the next over the length of the study. CV recovery may supplement resting and responsivity in the prediction of future development of CVD PMID:11239698

  8. Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual Machine

    E-print Network

    California at Santa Barbara, University of

    Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual jblynch@ucsd.edu Abstract The OpenCV framework is an open source project released under the BSD license will focus on the OpenCV framework, an open source project that furnishes developers with a series

  9. Processing in a transitional environment of CV and CK chondrites' parent bodies in the light of mineralogical and petrological analysis of NWA 1465 CV3 meteorite

    NASA Astrophysics Data System (ADS)

    Kereszturi, A.; Ormandi, Sz.; Jozsa, S.

    2015-05-01

    CV and CK chondrites might came from the same parent body from different depth values. To better constrain the conditions there, the composition, structure and chronology of mineral changes were analyzed in NWA 1465 CV3 chondrite meteorite. This sample represents an interesting part of the proposed ancient parent body where opposite to the generally poor altered state of CV3 chondrites, strong mechanical deformation produced elongated chondrules. All of the ingredients in the sample were flattened and fractured. After the chondrules settled down glass formation, opaque crystallization, fracturing and a second opaque generation followed by carbonate crystallization happened. The lack of fragments along the veins suggests no shearing movement happened, and the small number of weak shock signatures points to S2 level, and the oriented pressure in the parent body might come from burial induced pressure. The meteorite might came from a location in the CV-CK parent body where melting, and weak impact related fracturing and vein filling happened. In this an environment alteration of the primitive material started, possibly from a zone transitional between the shallow, less processed CV, and the deeper, more processed CK chondrites.

  10. Absolute properties of the spotted eclipsing binary star CV Bootis

    E-print Network

    Torres, Guillermo; Lacy, Claud H Sandberg

    2008-01-01

    We present new V-band differential brightness measurements as well as new radial-velocity measurements of the detached, circular, 0.84-day period, double-lined eclipsing binary system CV Boo. These data along with other observations from the literature are combined to derive improved absolute dimensions of the stars for the purpose of testing various aspects of theoretical modeling. Despite complications from intrinsic variability we detect in the system, and despite the rapid rotation of the components, we are able to determine the absolute masses and radii to better than 1.3% and 2%, respectively. We obtain M(A) = 1.032 +/- 0.013 M(Sun) and R(B) = 1.262 +/- 0.023 R(Sun) for the hotter, larger, and more massive primary (star A), and M(B) = 0.968 +/- 0.012 M(Sun) and R(B) = 1.173 +/- 0.023 R(Sun) for the secondary. The estimated effective temperatures are 5760 +/- 150 K and 5670 +/- 150 K. The intrinsic variability with a period about 1% shorter than the orbital period is interpreted as being due to modulatio...

  11. Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) Somaclones

    NASA Technical Reports Server (NTRS)

    Campbell, W. F.; Carman, J. G.; Hashim, Z. N.

    1990-01-01

    The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.

  12. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-05-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  13. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  14. [Characteristics of Papaver somniferum L. cv. ikkanshu cultivated in Izu].

    PubMed

    Iida, O; Sekine, T; Inoue, O; Yoshimatsu, K; Shimomura, K

    2000-01-01

    The seeds of Papaver somniferum L. cv. Ikkanshu were sown in November (Autumn sowing: AS) and March (Spring sowing: SS) in a field at Izu Experimental Station for Medicinal Plants of National Institute of Health Sciencs, and both AS and SS plants were cultivated to investigate their growth, opium yield and alkaloid content in the opium. Growing periods from the sowing to the opium harvest were approximately six months for AS plants and three months for SS plants. Sizes of plants and capsules in AS were bigger than those in SS, reflecting their growth period. Opium yields per an are in AS and SS were 212.09 g and 142.03 g, respectively. The opium was able to be collected four times in the AS plants though the SS plants ceased to exude opium after the second incision. Therefore higher yield of opium in AS plants seems to be attributed to an amount of opium in the third and fourth incision. Average morphine content in the total opium was 15.61% in AS plants and 15.04% in SS plants, and the estimated amounts of morphine per an are in AS and SS plants were 33.16 g and 21.38 g, respectively. PMID:11534131

  15. Impact-Induced Compaction in CV Chondrites: Exploring a Hidden Record with EBSD

    NASA Astrophysics Data System (ADS)

    Forman, L. V.; Bland, P. A.; Timms, N. E.; Benedix, G. K.

    2014-09-01

    We present impact-induced textural features found within the previously S1-classified CV, Allende. Features include high amounts of intragrain strain within the fine matrix grains, and moderate strain towards chondrule edges.

  16. Limited geographic distribution of the novel cyclovirus CyCV-VN

    PubMed Central

    Tan, Le Van; de Jong, Menno D.; Van Kinh, Nguyen; Trung, Nguyen Vu; Taylor, Walter; Wertheim, Heiman F. L.; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Uyen, Nguyen Hanh; Giri, Abhishek; Thi Thuy Chinh BKrong, Nguyen; Nghia, Ho Dang Trung; Farrar, Jeremy; Bryant, Juliet E.; Hien, Tran Tinh; Van Vinh Chau, Nguyen; van Doorn, H. Rogier

    2014-01-01

    A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600?CSF specimens from patients with suspected CNS infections in northern Vietnam, Cambodia, Nepal and The Netherlands were screened for the presence of CyCV-VN but all were negative. Sequence comparison and phylogenetic analysis between CyCV-VN and another novel cyclovirus recently identified in CSF from Malawian patients indicated that these represent distinct cycloviral species, albeit phylogenetically closely related. The data suggest that CyCV-VN has a limited geographic distribution within southern and central Vietnam. Further research is needed to determine the global distribution and diversity of cycloviruses and importantly their possible association with human disease. PMID:24495921

  17. THE BANANA PROJECT. V. MISALIGNED AND PRECESSING STELLAR ROTATION AXES IN CV VELORUM

    E-print Network

    Albrecht, Simon

    As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find ...

  18. Synergistic Degradation of CV1144-O Due to Ultraviolet Radiation and Heat

    Microsoft Academic Search

    JOSEPH E. HAFFKE; JOHN A. WOOLLAM

    Nu-Sil’s CV-1144-O is a silicone-based co-polymer used on spacecraft in LEO. CV-1144 has been spin-coated onto platinum sputter coated silicon wafers, and simulated LEO experiments made to study degradation. First, samples were exposed to ultraviolet radiation from a deuterium lamp with no additional heating. Next, samples were heated to 100°C during which there was no radiation exposure. Finally, samples were

  19. The comparison of CPU time consumption for image processing algorithm in Matlab and OpenCV

    Microsoft Academic Search

    Slavomir Matuska; Robert Hudec; Miroslav Benco

    2012-01-01

    In order to fill gap of growing demand for high efficient image and video processing, open source computer vision library (OpenCv) is way to deals with this task. Hence, this paper is about basic algorithm for image processing and their CPU time consumption in Matlab comparing with OpenCv. Algorithms are tested on images with resolution 3264×2448, 1920×1080, 1024×768 and 220×260.

  20. GpuCV: A GPU-Accelerated Framework for Image Processing and Computer Vision

    Microsoft Academic Search

    Yannick Allusse; Patrick Horain; Ankit Agarwal; Cindula Saipriyadarshan

    2008-01-01

    This paper presents briefly the state of the art of accelerating image processing with graphics hardware (GPU) and discusses\\u000a some of its caveats. Then it describes GpuCV, an open source multi-platform library for GPU-accelerated image processing and\\u000a Computer Vision operators and applications. It is meant for computer vision scientist not familiar with GPU technologies.\\u000a GpuCV is designed to be compatible

  1. Multi-camera calibration based on openCV and multi-view registration

    Microsoft Academic Search

    Xiao-Ming Deng; Xiong Wan; Zhi-Min Zhang; Bi-Yan Leng; Ning-Ning Lou; Shuai He

    2010-01-01

    For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the

  2. Detoxification of microcystin-LR in water by Portulaca oleracea cv.

    PubMed

    Isobe, Takatoshi; Okuhata, Hiroshi; Miyasaka, Hitoshi; Jeon, Bong-Seok; Park, Ho-Dong

    2014-03-01

    Microcystin-LR (0.02 ?g/ml) in the hydroculture medium of Portulaca oleracea cv., became below the detection level (<0.0001 ?g/ml) by HPLC analysis after 7 days. The toxicity of microcystin estimated with protein phosphatase inhibition assay, however, remained at 37% of the initial level, indicating that microcystin-LR was transformed by P. oleracea cv. into unknown compound(s) of lower toxicity. PMID:23999063

  3. Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV).

    PubMed

    Shi, Min; Chen, Ya-Feng; Huang, Fang; Liu, Peng-Cheng; Zhou, Xue-Ping; Chen, Xue-Xin

    2008-06-01

    Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with IkappaB-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy. PMID:18353418

  4. 76 FR 67209 - United States v. Grupo Bimbo S.A.B. de C.V., et al.; Proposed Final Judgment and Competitive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ...v. Grupo Bimbo S.A.B. de C.V., et al.; Proposed Final Judgment and Competitive...v. Grupo Bimbo S.A.B. de C.V., et al., Civil Action No. 1:11-cv-01857...Grupo Bimbo, S.A.B. de C.V., et al., Defendants. Case:...

  5. Absolute properties of the spotted eclipsing binary star CV Bootis

    E-print Network

    Guillermo Torres; Luiz Paulo R. Vaz; Claud H. Sandberg Lacy

    2008-09-12

    We present new V-band differential brightness measurements as well as new radial-velocity measurements of the detached, circular, 0.84-day period, double-lined eclipsing binary system CV Boo. These data along with other observations from the literature are combined to derive improved absolute dimensions of the stars for the purpose of testing various aspects of theoretical modeling. Despite complications from intrinsic variability we detect in the system, and despite the rapid rotation of the components, we are able to determine the absolute masses and radii to better than 1.3% and 2%, respectively. We obtain M(A) = 1.032 +/- 0.013 M(Sun) and R(B) = 1.262 +/- 0.023 R(Sun) for the hotter, larger, and more massive primary (star A), and M(B) = 0.968 +/- 0.012 M(Sun) and R(B) = 1.173 +/- 0.023 R(Sun) for the secondary. The estimated effective temperatures are 5760 +/- 150 K and 5670 +/- 150 K. The intrinsic variability with a period about 1% shorter than the orbital period is interpreted as being due to modulation by spots on one or both components. This implies that the spotted star(s) must be rotating faster than the synchronous rate, which disagrees with predictions from current tidal evolution models according to which both stars should be synchronized. We also find that the radius of the secondary is larger than expected from stellar evolution calculations by about 10%, a discrepancy also seen in other (mostly lower-mass and active) eclipsing binaries. We estimate the age of the system to be approximately 9 Gyr. Both components are near the end of their main-sequence phase, and the primary may have started the shell hydrogen-burning stage.

  6. Segmentation of kidney using C-V model and anatomy priors

    NASA Astrophysics Data System (ADS)

    Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

    2007-12-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

  7. [Image segmentation in tumor CT based on the improved C-V model].

    PubMed

    Zhang, Jianguo; Zhang, Rongguo; Xue, Fei; Liu, Kun

    2012-04-01

    Aiming at the shortcomings of slow convergence and inaccuracy segmentation in non-homogeneous images, improvements were made on the traditional C-V model in two aspects. Firstly, using a novel model based on local gradient, the initial contour of the C-V model was quickly moved near the target border, greatly reducing the evolution time. Secondly, combining the characteristics of GVF model from two directions to the target border, an adaptive velocity reconciling item was added for velocity equation of the C-V model to make the model converge to the true border. The segmentation experiments for liver tumors in CT showed that the proposed method could be effective. PMID:22616187

  8. Learning OpenCV---Computer Vision with the OpenCV Library (Bradski, G.R. et al.; 2008)[On the Shelf

    Microsoft Academic Search

    Alex Zelinsky

    2009-01-01

    This is an introductory textbook for teachers, students, professionals, and hobbyists who want to learn the basics of computer vision. The book is completely based around the OpenCV library, an open source project that started in 1999 by the computer-vision community. The authors of the text are among the principal contributors to this real-time library that has developed in C\\/C++

  9. VAX-FASTLINK: a high performance link between DEC and CV

    SciTech Connect

    Blackwell, K.O.; Corey, I.R.; Kucala, J.M.; Shimamoto, F.Y.

    1985-06-10

    The environment at LLNL requires a communications capability between our network of DEC computers (VAX and PDP11) and CV CADDS 4X. We will describe a communications link which meets our requirements using standard CV communications products and a suite of hardware and software on the DEC side. The data flow and functional control provided by this link will be described. There will also be a discussion of our implementation of this project. There will be some performance data presented also. Finally, we will describe the CAE/CAD/CAM environment that this link operates in at LLNL.

  10. Heterogeneity in host-parasitoid interactions: 'Aggregation of risk' and the 'CV(2) > 1 Rule'.

    PubMed

    Taylor, A D

    1993-11-01

    In host-parasitoid interactions the coefficient of variation (CV) of the risk of parasitism measures, at least approximately, the stabilizing influence of many forms of heterogeneity. This realization emphasizes the underlying similarity of these heterogeneities, and should end two decades of confusion about how to study them in nature. The first applications of this approach suggest that roughly one-third of systems studied show substantial measurable heterogeneity. Proper use of the CV measure, however, requires that the density-dependent processes linking this static phenomenon to temporal population dynamics also be examined; this has yet to be done for any system. PMID:21236211

  11. The chemical composition of suberin in apoplastic barriers affects radial hydraulic conductivity differently in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix)

    Microsoft Academic Search

    Lukas Schreiber; Rochus Franke; Klaus-Dieter Hartmann; Kosala Ranathunge; Ernst Steudle

    2005-01-01

    Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degrada- tion (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were ana- lysed quantitatively by gas chromatography and mass spectrometry. In corn, suberin was determined for isolated endodermal (ECW) and rhizo-hypodermal (RHCW) cell

  12. Africana Video Collection Africana Documentaries..................page 1

    E-print Network

    , people in government -- "We are celebrating 100 years since the abolition of slavery in Brazil, what does the abolition of slavery mean to you?" THE ACADEMY, WINDHOEK, NAMIBIA CALL NUMBER: 378.6881 A168a vhs 53 minutes

  13. Accepted Manuscript REE and Hf distribution among mineral phases in the CV-CK clan: a way to

    E-print Network

    Claeys, Philippe

    Accepted Manuscript REE and Hf distribution among mineral phases in the CV-CK clan: a way., REE and Hf distribution among mineral phases in the CV-CK clan: a way to explain present-day Hf the content, and all legal disclaimers that apply to the journal pertain. #12;REE and Hf distribution among

  14. Abstract--There are two types of drivers in production machine systems: constant velocity (CV) motor and servo-motor.

    E-print Network

    Zhang, WJ "Chris"

    ) motor and servo-motor. If a system contains two drivers or more, among which some are of the CV motor while the other are the servo-motor, the system has the so-called hybrid driver architecture and servo-motor. If a system contains two or more drivers, among which some are of the CV motor while

  15. Electronic Journal of Africana Bibliography

    NSDL National Science Digital Library

    "EJAB is a refereed online journal of bibliographies on any aspect of Africa, its peoples, their homes, cities, towns, districts, states, countries, regions, including social, economic sustainable development, creative literature, the arts, and the Diaspora." The site currently has three bibliographies: Guides, Collections and Ancillary Materials to African Archival Resources in the US; Foreign Periodicals on Africa; and Medical/Health Periodicals and Books on Africa. Combined, they contain over 2100 entries. Anyone studying Africa will undoubtedly find numerous important resources.

  16. Modeling the structure of CV formation and expectations : The commercial retail real estate sector

    Microsoft Academic Search

    2007-01-01

    Purpose – The paper aims to form system dynamics modeling in introduced in conjunction with econometric analysis and planned scenario analysis which will uniquely structure the process whereby the ex ante capital values of the prime retail real estate sector. Design\\/methodology\\/approach – The integrated system dynamics model investigates the structural factors affecting a unique expectation-centered capital value (CV) formation of

  17. Correlation Between the Size of Three-Dimensional Nanoclusters and C-V Characteristics

    NASA Astrophysics Data System (ADS)

    Kovalevskii, A.; Strogova, A.; Strogova, N.; Babushkina, N.

    2013-05-01

    We have considered size effects of silicon/germanium nanoclusters on the hysteresis of C-V characteristics of metal-insulator-semiconductor structures where silicon nitride doped by lanthanum is uses as a dielectric. Critical size of 70 nm is suggested for nanoclusters for efficient performance of non-volatile memory.

  18. The influence of nitrate nutrition on H + efflux by young rape plants ( Brassica napus cv. emerald)

    Microsoft Academic Search

    H. Moorby; P. H. Nye; R. E. White

    1985-01-01

    Changes in pH around the roots of young rape plants (Brassica napus cv. emerald) were studied using a nutrient film technique that allowed part or whole of the root system to be subjected to specific nutrient treatments. The rapidity and direction of pH change was assessed by imbedding absorbing roots in a thin film of agar containing bromocresol purple. When

  19. Updated 9/24/12 Marx CV 1 DAVID M. MARX

    E-print Network

    Gallo, Linda C.

    Updated 9/24/12 Marx CV 1 DAVID M. MARX SDSU Department of Psychology 5500 Campanile Drive San: dmarx@sciences.sdsu.edu EDUCATION · Ph.D. (2001) Harvard University, Social Psychology (1998-01) Dissertation: Minority role models: Improving minority students' test performance in the face of negative

  20. Replacement of soya in pig diets with white lupine cv. Butan

    Microsoft Academic Search

    Z. Zralý; B. Písa?íková; M. Tr?ková; M. Doležal; J. Thiemel; J. Simeonovová; M. J?zl

    The purpose of the present study was to evaluate the effect of soya replacement (50 or 100%) with whole (WL) or dehulled seeds (DL) of white lupine cv. Butan in the diets for market pigs. The experiment was performed on 50 pigs in equal numbers of barrows and gilts with the initial mean body weight (BW) of 18.3 ± 2.1

  1. EST-SSR MARKERS FROM FRAGARIA VESCA L. CV. YELLOW WONDER

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fourteen microsatellite primer pairs were developed from a cDNA library of Fragaria vesca cv. Yellow Wonder. Transferability to 13 species of Fragaria ranged from 71% in diploid species F. gracilis Losinsk., F. iinumae Makino, F. nilgerrensis Schltdl. ex J. Gay and F. nipponica Makino to 100% in the...

  2. CV dtaill et rapport d'activit de Lapo BOSCHI Lapo BOSCHI

    E-print Network

    Boschi, Lapo

    CV détaillé et rapport d'activité de Lapo BOSCHI 1 Lapo BOSCHI Maitre de conférences Laboratoire iSTeP, UMR 7193 UPMC-CNRS Université Pierre et Marie Curie 4, place Jussieu Case 129, T.46-00, Et.2 75252 Paris Cedex 05 Téléphone : +33(0)144275243 Email : lapo

  3. Phenotypical, Sanitary and Ampelometric Variability within the Population of cv. Plavac Mali (Vitis vinifera L.)

    Microsoft Academic Search

    Jasminka KAROGLAN; Ivan PEZO

    Summary One hundred and sixty individual vines representative of the entire growing region for cv. Plavac Mali (Middle and Southern Dalmatia) were ampelographically analyzed in situ during a period of four years. High phenotypical variability was determined in: the maturity period (35.6% \\

  4. State of Alaska v. Lubchenco, et al. Case No. 3:10-CV-00271-TMB

    E-print Network

    Fisheries Service (NMFS) has met the first deadline to date in the U. S. District Court's March 5, 2012 DISTRICT COURT FOR THE DISTRICT OF ALASKA STATE OF ALASKA, ) ) CASE NO. 3:10-CV-00271-TMB Plaintiffs to the Court's March 5, 2012 Order (Docket 142) at 13, submit the following status report. The National Marine

  5. Sarah E. Hill CV 1 S A R A H E. H I L L

    E-print Network

    Cooper, Brenton G.

    .D., Psychology: December, 2006 o Dissertation Title: Dissatisfied by Design: The Evolution of Discontent oSarah E. Hill ­ CV 1 S A R A H E. H I L L OFFICE · TEXAS CHRISTIAN UNIVERSITY · PSYCHOLOGY DEPT Psychology Minor Concentration(s): Evolutionary Biology / Quantitative Methods The University of Wisconsin

  6. Automatic tracking of red blood cells in micro channels using OpenCV

    NASA Astrophysics Data System (ADS)

    Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

    2013-10-01

    The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

  7. OpenCV compatible real time processor for background foreground identification

    Microsoft Academic Search

    M. Genovese; E. Napoli; N. Petra

    2010-01-01

    The background identification methods are used in many fields like video surveillance and traffic monitoring. In this paper we propose a hardware implementation of the Gaussian Mixture Model algorithm able to perform background identification on HD images. The proposed circuit is based on the OpenCV implementation, particularly suited to improve the initial background learning phase. Bit-width has been optimized in

  8. Registered Localization Method-based on OpenCV for Augmented Reality

    Microsoft Academic Search

    Jun Dai; Li-fen Zhang

    2011-01-01

    Augmented Reality is a kind of technique combining a virtual world with a real environment. This paper tells that OpenCV is applied to the realization of Registration Technique which is key to Augmented Reality. It studies the implementation of the camera calibration, the extraction of characteristic target and its recognition. This paper improved the efficiency of the exploitation and laid

  9. Teaching image processing and pattern recognition with the Intel OpenCV library

    Microsoft Academic Search

    Adam Kozlowski; Aleksandra Królak

    2009-01-01

    In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image

  10. Recognition assisted dynamic surveillance system based on OSGi and OpenCV

    Microsoft Academic Search

    Jenq-Shiou Leu; Wei-Hsiang Lin; Min-Chieh Yu; Hung-Jie Tzeng

    2011-01-01

    Thanks to the Internet development, a surveillance user can monitor the distant events via a browser on a computer. These events can be even viewed on a mobile phone with the assistance from modern mobile networks. Besides, two well-known platforms - OSGi and OpenCV are widely used for application construction. OSGi is constructed to provide a service platform with high

  11. Phytoremediation of 4,4'-thiodiphenol (TDP) and other bisphenol derivatives by Portulaca oleracea cv.

    PubMed

    Okuhata, Hiroshi; Ninagawa, Masahiko; Takemoto, Naomichi; Ji, Hezhe; Miyasaka, Hitoshi; Iwamoto, Ai; Nagae, Masaki; Ishibashi, Yasuhiro; Arizono, Koji

    2013-01-01

    4,4'-Thiodiphenol (TDP) is a bisphenol derivative, and there has been no report on TDP removal by any plants or pure bacterial cultures. The removal of TDP by Portulaca oleracea cv., a floricultural herbal plant, was examined with a hydroculture system, and 97% of TDP was removed after 4 days culture. PMID:23040992

  12. PHYSICAL CHEMISTRY OF REFRACTORY INCLUSIONS IN CV3 CHONDRITES. L. Grossman, Dept. Geophys. Sci. and

    E-print Network

    Grossman, Lawrence

    + anorthite) inclusions in CV3 chondrites match those of the highest temperature condensates (e.g., 1500-1450K, melilite, anorthite and fassaite. Melilite is a solid solution of gehlenite, Ge (Ca2Al2SiO7) and åkermanite in Type Bs show that cooling rates were fast enough, ~ 0.5 to anorthite crystallized after

  13. Safe, Effective and Easily Reproducible Fusion Technique for CV Junction Instability

    PubMed Central

    Sannegowda, Raghavendra Bakki

    2015-01-01

    Introduction: The Craniovertebral junction (CVJ) refers to a bony enclosure where the occipital bone surrounds the foramen magnum, the atlas and the axis vertebrae. Because of the complexity of structures, CVJ instability is associated with diagnostic and therapeutic problems. Posterior CV fusion procedures have evolved a lot over the last couple of decades. There has been a lookout for one such surgical procedure which is inherently safe, simple, easily reproducible and biomechanically sound. In our study, we present the initial experience the cases of CV junction instrumentation using O-C1-C2 screw & rod construct operated by the author. Aims and Objectives: The current study is a descriptive analysis of the cases of CVJ instability treated by us with instrumentation using O-C1-C2 screw and rod construct fusion technique. Materials and Methods: It is a retrospective, analytical study in which cases of CV junction instability operated by the author between January 2010 to March 2014 were analysed using various clinical, radiological and outcome parameters. Conclusion: CV junction instrumentation using O-C1-C2 screw and rod construct fusion technique proved to be safe, effective, easily reproducible and biomechanically sound technique which can be adopted by all surgeons who may be at any stage of their learning curve. PMID:25954660

  14. THE VALUE OF LUPINUS ALBUS L. CV. AU HOMER AS A WINTER COVER CROP FOR COTTON

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Successful cotton (Gossypium hirsutum L.) management in the southeastern USA with conservation tillage requires the utilization of winter cover crops to increase organic matter in the top 5 cm of the soil. The objective of our research was to test the newly-developed bitter white lupin cv. `AU Home...

  15. Hydraulic architecture of plants of Helianthus annuus L. cv. Margot: evidence for plant segmentation in herbs

    Microsoft Academic Search

    M. A. Lo Gullo; L. Castro Noval; S. Salleo; A. Nardini

    2004-01-01

    The hydraulic architecture of sunflower (Helianthus annuus L. cv. Margot) was studied in terms of the partitioning of the hydraulic conductance (Kleaf )o f leaves inserted at progressively more apical nodes both in growing plants (GP) and in plants at full anthesis (mature plants, MP). Leaf conductance to water vapour (gL), leaf water potential (WL), leaf water potential at zero

  16. Indirect measurements of Brachiaria brizantha cv. Marandu fermentable cell wall sugars for second generation biofuels production.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Results of a study conducted to evaluate the possibility of using IVDMD values of B. brizantha cv. Marandu to predict cell wall sugars that would be available in a biorefinery for ethanol production are reported. The study was conducted based on the similarity between rumen enzymes and those used i...

  17. Chemical changes during the development and ripening of the fruit of Cucumis melo (Cv. Makdimon)

    Microsoft Academic Search

    Youming Wang; S. Grant Wyllie; David N. Leach

    1996-01-01

    Changes in the aroma volatiles, free amino acids, sugars, principal acids, and soluble minerals were studied during the development and ripening of the fruit of Cucumis melo L. Reticulatis group cv. Makdimon. Ethyl acetate was the most abundant volatile produced during the final growth stage, but ethanol was the major volatile found in immature fruit. Most of the free amino

  18. M. Bahrami Fluid Mechanics (S 09) Integral Relations for CV 8 The angular momentum

    E-print Network

    Bahrami, Majid

    M. Bahrami Fluid Mechanics (S 09) Integral Relations for CV 8 The angular momentum A control volume analysis can be applied to the angular momentum, by letting B equal to angular momentum vector H. If O is the point about which moments are desired, the angular moment about O

  19. Effect of plant age on element concentrations in parts of Desmodium intortum cv. Greenleaf

    Microsoft Academic Search

    C. Johansen

    1978-01-01

    The distribution of some nutrient elements in parts of Desmodium intortum cv. Greenleaf plants of different age was studied in order to determine the most appropriate sampling technique for chemical analysis of nutrient status. When expressed on a dry weight basis, concentrations of N, P, K, S, Ca, Mg, Al, B and Mn in plant parts of similar physiological age

  20. Improved plant regeneration from shed microspore culture in barley ( Hordeum vulgare L.) cv. igri

    Microsoft Academic Search

    A. Ziauddin; E. Simion; K. J. Kasha

    1990-01-01

    This report describes rapid regeneration of green plants from microspores of the barley cultivar Igri. Use of 0.3 M mannitol during maceration and isolation was essential for response from mechanically isolated microspores of barley cv. Igri grown under our conditions. A shed microspore culture system proved to be simple and gave a fast response; plants were obtained as early as

  1. EXPERIMENTAL DEMONSTRATION OF RESET CONTROL DESIGN1 C.V. Hollot,4

    E-print Network

    Massachusetts at Amherst, University of

    of Massachusetts, Amherst; Presently, a Servo Design Engineer, NeoMagic Corporation, Santa Clara, CA 95054; yzhengEXPERIMENTAL DEMONSTRATION OF RESET CONTROL DESIGN1 Y. Zheng,2 Y. Chait,3 C.V. Hollot,4 M/phase margins. To the best of our knowledge, this is the first experimental demonstration of reset control

  2. OpenCV WebCam Applications in an Arduino-based Rover

    E-print Network

    Boyer, Edmond

    OpenCV WebCam Applications in an Arduino-based Rover Valeria Loscr´i1 , Nathalie Mitton1 , Emilio Arduino-based Rovers with characteristics of re-programmability, modularity in terms of type and number them. In this paper, we build an Arduino-based platform, that presents some im- portant characteristics

  3. Green beans (Phaseolus vulgaris cv. Gallatin 50): Effects of plant population density on yield and quality

    Microsoft Academic Search

    R. J. Field; S. Nkumbula

    1986-01-01

    The yield and quality of green beans (Phaseolus vulgaris L. cv. Gallatin 50) was determined after sowing the crop at 150, 300, 380, and 450 mm between-row distances and within-row distances of 70 or 140 mm. Total pod yield and the yield of processable pods ( > 50 mm in length) showed significant quadratic responses to increasing plant population density.

  4. Isolation and Characterization of CvIV4: A Pain Inducing ?- Scorpion Toxin

    PubMed Central

    Rowe, Ashlee H.; Xiao, Yucheng; Scales, Joseph; Linse, Klaus D.; Rowe, Matthew P.; Cummins, Theodore R.; Zakon, Harold H.

    2011-01-01

    Background Among scorpion species, the Buthidae produce the most deadly and painful venoms. However, little is known regarding the venom components that cause pain and their mechanism of action. Using a paw-licking assay (Mus musculus), this study compared the pain-inducing capabilities of venoms from two species of New World scorpion (Centruroides vittatus, C. exilicauda) belonging to the neurotoxin-producing family Buthidae with one species of non-neurotoxin producing scorpion (Vaejovis spinigerus) in the family Vaejovidae. A pain-inducing ?-toxin (CvIV4) was isolated from the venom of C. vittatus and tested on five Na+ channel isoforms. Principal Findings C. vittatus and C. exilicauda venoms produced significantly more paw licking in Mus than V. spinigerus venom. CvIV4 produced paw licking in Mus equivalent to the effects of whole venom. CvIV4 slowed the fast inactivation of Nav1.7, a Na+ channel expressed in peripheral pain-pathway neurons (nociceptors), but did not affect the Nav1.8-based sodium currents of these neurons. CvIV4 also slowed the fast inactivation of Nav1.2, Nav1.3 and Nav1.4. The effects of CvIV4 are similar to Old World ?-toxins that target Nav1.7 (AahII, BmK MI, LqhIII, OD1), however the primary structure of CvIV4 is not similar to these toxins. Mutant Nav1.7 channels (D1586A and E1589Q, DIV S3–S4 linker) reduced but did not abolish the effects of CvIV4. Conclusions This study: 1) agrees with anecdotal evidence suggesting that buthid venom is significantly more painful than non-neurotoxic venom; 2) demonstrates that New World buthids inflict painful stings via toxins that modulate Na+ channels expressed in nociceptors; 3) reveals that Old and New World buthids employ similar mechanisms to produce pain. Old and New World ?-toxins that target Nav1.7 have diverged in sequence, but the activity of these toxins is similar. Pain-inducing toxins may have evolved in a common ancestor. Alternatively, these toxins may be the product of convergent evolution. PMID:21887265

  5. 77 FR 5574 - United States v. Grupo Bimbo, S.A.B. de C.V., et al.; Public Comment and Response on Proposed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ...Grupo Bimbo, S.A.B. de C.V., et al.; Public Comment and Response on Proposed...Grupo Bimbo, S.A.B. de C. V., et al., Civil Action No. 1:11-cv-01857...Grupo Bimbo, S.A.B. de C.V., et al. Defendants. CASE NO.:...

  6. All regimes mobility extraction using split C-V technique enhanced with charge-sheet model

    NASA Astrophysics Data System (ADS)

    Hubert, Q.; Carmona, M.; Rebuffat, B.; Innocenti, J.; Masson, P.; Masoero, L.; Julien, F.; Lopez, L.; Chiquet, P.

    2015-09-01

    In this paper, carrier effective mobility is accurately extracted from weak to strong inversion and from ohmic to saturation regimes by pairing the split C-V technique with charge-sheet model. In weak inversion, both electron and hole effective mobility are found to be constant and VDS independent. Moreover, effective mobility extracted by this new method is modeled in all regimes using already published models extended up to the saturation regime.

  7. Calcium Isotopic Ratios and Rare Earth Element Abundances in Refractory Inclusions from Allende CV3 Chondrite

    Microsoft Academic Search

    Shichun Huang; Juraj Farkaš; Gang Yu; Michail I. Petaev; Stein B. Jacobsen

    Refractory inclusions in primitive meteorites are the oldest objects formed in the Solar System. They exhibit large mass-dependent isotopic effects in many elements, including Si, Mg and Ca, indicative of their complex origin. We report data for both mass-dependent (?) and mass-independent (?) Ca isotopic effects, and rare earth element (REE) abundances in six refractory inclusions from the Allende CV3

  8. About the determination of the Schottky barrier height with the CV method

    Microsoft Academic Search

    V. G. Bozhkov; N. A. Torkhov; A. V. Shmargunov

    2011-01-01

    On the basis of general notions about Schottky barrier contacts (SBC) with the insulating layer and interface states (ISs) communicating with semiconductor and metal (when their influence results in the linear bias-dependence of the barrier height and the ideality factor n=const), it is shown that the barrier height determined with C-V method is defined with a simple expression: ?bc=n?b0-(n-1)(?s+V2), practically

  9. About the determination of the Schottky barrier height with the CV method

    Microsoft Academic Search

    V. G. Bozhkov; N. A. Torkhov; A. V. Shmargunov

    2011-01-01

    On the basis of general notions about Schottky barrier contacts (SBC) with the insulating layer and interface states (ISs) communicating with semiconductor and metal (when their influence results in the linear bias-dependence of the barrier height and the ideality factor n=const), it is shown that the barrier height determined with C-V method is defined with a simple expression: phibc=nphib0-(n-1)(phis+V2), practically

  10. Nano-silver pulse treatments improve water relations of cut rose cv. Movie Star flowers

    Microsoft Academic Search

    Peitao Lü; Jinping Cao; Shenggen He; Jiping Liu; Hongmei Li; Guiping Cheng; Yuelian Ding; Daryl C. Joyce

    2010-01-01

    Effects of pulse treatments with nano-silver (NS) particle (2–5nm diam) solutions on the vase life of cut rose cv. Movie Star flowers were investigated. Pulse treatments of NS at 50, 100 and 250mgL?1 were for 1h. Stems were then transferred to deionized water (DI) and evaluated daily for vase life and quality. The 250mgL?1 NS pulse treatment was phytotoxic. However,

  11. Nano-silver pulse treatments inhibit stem-end bacteria on cut gerbera cv. Ruikou flowers

    Microsoft Academic Search

    Jiping Liu; Shenggen He; Zhaoqi Zhang; Jinping Cao; Peitao Lv; Sudan He; Guiping Cheng; Daryl C. Joyce

    2009-01-01

    Nanometer-sized silver (Ag+) particles (NS) are used in various applications as an anti-microbial. Effects of NS (2–5nm diam.) pulse solution treatments on vase life of cut gerbera (Gerbera jamesonii) cv. Ruikou flowers were investigated. Compared with the control [pulsed with deionised water (DI) and subsequently held in DI] pulsing for 24h with 5mg\\/L NS solution followed by holding in DI

  12. Impact of salinity on the fruit yield of mango ( Mangifera indica L. cv. ‘Osteen’)

    Microsoft Academic Search

    V. H. Durán Zuazo; A. Mart??nez Raya; J. Aguilar Ruiz

    2004-01-01

    The impact of saline irrigation water of different chloride and sodium concentrations on the fruit yield of field-grown mango (Mangifera indica L. cv. ‘Osteen’) was evaluated for 4 years (1996–1999). Tree performance was evaluated for two rootstocks (Gomera-1 or Gomera-3) and at water salinities having an electrical conductivity of 1.02, 1.50, 2.00 or 2.50dSm?1. The results demonstrated that Gomera-1 was

  13. The Influence of Fungicide Sprays on Infection Of Apple cv. Bramley's Seedling By Nectria galligena

    Microsoft Academic Search

    Louise R. Cooke

    1999-01-01

    In 1990, a long-term trial was initiated by planting young apple trees, cv. Bramley's Seedling, inoculated, at single sites in the leader shoots, with Nectria galligena. The effect of spring–summer and autumn fungicide spray programmes, applied during 1991–1993, on the development of new cankers was assessed up to May 1994. Spring–summer fungicide programmes, applied as for the control of apple

  14. Organelle genome stability in anther-derived doubled haploids of wheat ( Triticum aestivum L., cv. ‘Moisson’)

    Microsoft Academic Search

    A. Rode; C. Hartmann; M. Dron; E. Picard; F. Ouetier

    1985-01-01

    Chloroplast and mitochondrial compartments of a parental line of wheat (Triticum aestivum L., cv. ‘Moisson’) and its anther-derived doubled haploid lines have been analyzed and compared on the basis of their DNA restriction patterns. The results obtained show that no noticeable difference can be detected between doubled haploid lines and parental line at the level of ctDNA and mtDNA organization.

  15. Chelator-Enhanced Lead Accumulation in Agropyron Elongatum cv. Szarvasi-1 in Hydroponic Culture

    Microsoft Academic Search

    Ildikó Vashegyi; László Lévai; Ferenc Fodor

    2011-01-01

    Hydroponic culture was applied to compare the efficiency of K2EDTA and citrate in mobilizing Pb for accumulation in Agropyron elongatum cv. Szarvasi-1 and their effects on some physiological characteristics of the plants. The plants were grown in nutrient solutions containing 0, 10, and 100 ?M Pb(NO3)2 combined with chelating agents added to the nutrient solutions after 21 days of growth,

  16. Extremely Na and Cl-rich chondrule from the CV3 carbonaceous chondrite Allende

    Microsoft Academic Search

    G. J. Wasserburg; I. D. Hutcheon; J. Aléon; E. C. Ramon; A. N. Krot; K. Nagashima; A. J. Brearley

    2011-01-01

    We report on a study of Al3509, a large Na- and Cl-rich, radially-zoned object from the oxidized CV carbonaceous chondrite Allende. Al3509 consists of fine-grained ferroan olivine, ferroan Al-diopside, nepheline, sodalite, and andradite, and is crosscut by numerous veins of nepheline, sodalite, and ferroan Al-diopside. Some poorly-characterized phases of fine-grained material are also present; these phases contain no significant H2O.

  17. Study on on-line measurement system of stone slabs based on OpenCV

    Microsoft Academic Search

    Zhao Min; Na Lihong

    2010-01-01

    An on-line measurement system of stone slabs based on CCD technology is constituted by using the CCD camera, image acquisition card, computer and so on. The on-line measurement system for the stone slab is realized by applying camera calibration and image processing algorithms. Under VC++6.0 condition, camera calibration is realized by calling OpenCV1.0 library function and stone slab contour is

  18. Research of Driver Eye Features Detection Algorithm Based on OpenCV

    Microsoft Academic Search

    Shifeng Hu; Zuhua Fang; Jie Tang; Hongbing Xu; Ying Sun

    2010-01-01

    This paper proposes a driver fatigue eye features detection algorithm based on OpenCV image processing and computer vision development platform. This algorithm localizes eye-area and detects its state based on rough to accurate thought, and can localize eye pupils in eye-open state accurately, which has significance to decrease traffic accidents. The experiment shows this algorithm can detect drivers' eye states

  19. The calibration of a binocular intersection VMD measurement system based on OpenCV

    Microsoft Academic Search

    Yang Zhenhua; He Lingsong; Bei Lei

    2010-01-01

    In this paper we present a two-step stereo calibration method of binocular intersection VMD measurement system based on OpenCV. First, calibrate the two cameras separately, and get the camera's intrinsic parameters. Second, solve the extrinsic parameters from the stereo image pairs and the camera's intrinsic parameters. The experiment shows that this two-step calibration method for binocular intersection VMD measurement system

  20. Highly optimized implementation of OpenCV for the Cell Broadband Engine

    Microsoft Academic Search

    Hiroki Sugano; Ryusuke Miyamoto

    2010-01-01

    Recently, real-time processing of image recognition is required for embedded applications such as automotive applications, robotics, entertainment, and so on. To realize real-time processing of image recognition on such systems we need optimized libraries for embedded processors. OpenCV is one of the most widely used libraries for computer vision applications and has many functions optimized for Intel processors, but no

  1. Isolation and culture of Lens culinaris Medik. cv. Eston epicotyl protoplasts to calli

    Microsoft Academic Search

    Kevin L. Rozwadowski; Praveen K. Saxena; J. King

    1990-01-01

    Protoplasts of Lens culinaris Medik. cv. Eston were isolated from epicotyl tissues of seedlings grown on Murashige & Skoog basal medium. For isolating the protoplasts, epicotyl tissues were digested for 12–14 h at 25°C in an isolation mixture (pH 5.7) containing 1% Cellulase RS, 0.5% Driselase, 0.25% Pectolyase Y23, 0.2M calcium chloride, 10 mM mannitol and 10 mM MES. Protoplasts

  2. Agrobacterium -mediated transformation using embryogenic calli in Satsuma mandarin ( Citrus unshiu Marc.) cv. Miyagawa wase

    Microsoft Academic Search

    Seong Beom Jin; Jeong Won Park; Hyeon Jin Sun; Su Hyun Yun; Hyo Yeon Lee; Dong Sun Lee; Quan Chun Hong; Yong Woo Kim; Key Zung Riu; Jae Hoon Kim

    2011-01-01

    Agrobacterium-mediated transformation in Satsuma mandarin (Citrus unshiu Marc.) cv. Miyagawa wase was achieved with reasonable transformation efficiency of about 22%, which was the percentage of\\u000a transgenic plantlets confirmed by genomic PCR (37 plantlets\\/168 hygromycin-resistant calli). Embryogenic calli of Miyagawa\\u000a wase were infected with Agrobacterium tumefaciens strain EHA105 harboring binary vector pCAMBIA1300 that contained miraculin gene (a taste-modifying protein) and hygromycin

  3. How to solve nonlinear operator equation A(v2)+Cv=f

    Microsoft Academic Search

    Cui Minggen; Chen Zhong

    2004-01-01

    In this paper, we discuss the problems of how to solve linear operator equation in Hilbert spaces and a kind of nonlinear operator equation A(v2)+Cv=f in reproducing kernel space W21[a,b]. For linear operator equation, we obtain a criterion about the existence of solutions. If it has solutions, we get the analytic representation of its minimum norm solution and the structure

  4. Genetic transformation of Cavendish banana ( Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment

    Microsoft Academic Search

    D. K. Becker; B. Dugdale; M. K. Smith; R. M. Harding; J. L. Dale

    2000-01-01

    An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature\\u000a male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or

  5. Alkaloid Production in Tissue Cultures of Papaver somniferum L. cv. Office95

    Microsoft Academic Search

    Esin Akçam Oluk

    2006-01-01

    The capacity of alkaloid synthesis was examined in embryogenic callus tissues of Turkish opium poppy, Papaver somniferum L cv. office-95. Eight months old cultures grown in hormone-free MS were examined for alkaloid content. They were found to produce codeine at a level of 1.2 × 10 -2 , the baine 2.5 × 10 -3 , noscapine 2.2 × 10-3, morphine

  6. SALT spectral observations of a new, bright, southern CV: MASTER OT J142023.5-485540

    NASA Astrophysics Data System (ADS)

    Gulbis, A. A. S.; Kotze, M. M.; Kotze, E. J.; Worters, H. L.; Buckley, D. A. H.; O'Donoghue, H.; Shara, M.

    2013-07-01

    On 2013-06-24.063 UT, the Southern African Large Telescope (SALT; Buckley et al. 2006; O’Donoghue et al. 2006) observed MASTER OT J142023.5-485540 (RA=14h 20m 23.5s; Dec=-48d 55m 40s). This outbursting CV was discovered by MASTER-ICATE on 2013-06-08.048 UT and followup observations were requested in ATEL #5144.

  7. Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO

    Microsoft Academic Search

    Andreas Albert; Vipaporn Sareedenchai; Werner Heller; Harald K. Seidlitz; Christian Zidorn

    2009-01-01

    Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent\\u000a field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity\\u000a of the tissues involved. Factors causing these variations were investigated by climate

  8. In vitro selection of endosulfan-tolerant strains of Brassica compestris (cv. Brown Sarson)

    Microsoft Academic Search

    K. R. Arya; R. C. Gupta; B. D. Semwal; M. U. Beg

    1994-01-01

    Endosulfan tolerant lines of mustard (Brassica campestris cv. Brown Sarson) have been developed through tissue culture methods. Cotyledonary expiants excised from eight day old in vitro grown seedlings were used for inducing callus. Fast growing friable callus was then transferred to MS medium containing (0.1–2.0 ugl-1) endosulfan for selection. Five alternating exposures with and without endosulfan containing medium yielded an

  9. Numerical C-V calculations of CdTe/CdS thin films solar cell

    NASA Astrophysics Data System (ADS)

    Castillo-Alvarado, F. L.; Avendaño-Lopez, J.; Contreras-Puente, G.; Vigil, O.; Vidal, J.; de Gante, P.; Rojas-Hernandez, E.; Hernandez-Contreras, H.

    2000-03-01

    We have calculated the band discontinuites and interface charge density of the heterojunction CdTe/CdS, the calculations are based in the C-V matching method. Our solar cells are builted with thin films done by close spaced sublimation. The significance of the simultaneous determinations of the band discontinuites and interface charges of these solar cells is discussed. Work partially supported by CONACYT, Mexico, proyect G27713A

  10. Phylogenetic position of Mesorhizobium huakuii subsp. rengei, a Symbiont of Astragalus sinicus cv. Japan

    Microsoft Academic Search

    Sukma Nuswantara; Makoto Fujie; Takashi Yamada; Wanda Malek; Masaki Inaba; Yoshinobu Kaneko; Yoshikatsu Murooka

    1999-01-01

    The phylogenetic position of Rhizobium huakuii bv. renge, a symbiont of Astragalus sinicus cv. Japan (renge-sou), was studied. The following phylogenetic approaches were used: restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR) analysis of a full-length 16S rDNA fragment, 16S rDNA analysis of the first 300-bp sequence, bacteriophage typing, and amplification of the genomic region by random primer. All the

  11. Heavily metamorphosed clasts from the CV chondrite breccias Mokoia and Yamato-86009

    NASA Astrophysics Data System (ADS)

    Jogo, Kaori; Nagashima, Kazuhide; Hutcheon, Ian D.; Krot, Alexander N.; Nakamura, Tomoki

    2012-12-01

    Abstract- Metamorphosed clasts in the CV carbonaceous chondrite breccias Mokoia and Yamato-86009 (Y-86009) are coarse-grained, granular, polymineralic rocks composed of Ca-bearing (up to 0.6 wt% CaO) ferroan olivine (Fa34-39), ferroan Al-diopside (Fs9-13Wo47-50, approximately 2-7 wt% Al2O3), plagioclase (An37-84Ab63-17), Cr-spinel (Cr/(Cr + Al) = 0.19-0.45, Fe/(Fe + Mg) = 0.60-0.79), nepheline, pyrrhotite, pentlandite, Ca-phosphate, and rare grains of Ni-rich taenite; low-Ca pyroxene is absent. Most clasts have triple junctions between silicate grains, indicative of prolonged thermal annealing. Based on the olivine-spinel and pyroxene thermometry, the estimated metamorphic temperature recorded by the clasts is approximately 1100 K. Few clasts experienced thermal metamorphism to a lower degree and preserved chondrule-like textures. The Mokoia and Y-86009 clasts are mineralogically unique and different from metamorphosed chondrites of known groups (H, L, LL, R, EH, EL, CO, CK) and primitive achondrites (acapulcoites, brachinites, lodranites). On a three-isotope oxygen diagram, compositions of olivine in the clasts plot along carbonaceous chondrite anhydrous mineral line and the Allende mass-fractionation line, and overlap with those of the CV chondrule olivines; the ?17O values of the clasts range from about -4.3‰ to -3.0‰. We suggest that the clasts represent fragments of the CV-like material that experienced metasomatic alteration, high-temperature metamorphism, and possibly melting in the interior of the CV parent asteroid. The lack of low-Ca pyroxene in the clasts could be due to its replacement by ferroan olivine during iron-alkali metasomatic alteration or by high-Ca ferroan pyroxene during melting under oxidizing conditions.

  12. Antibacterial and Antioxidant Activities of Banana (Musa, AAA cv. Cavendish) Fruits Peel

    Microsoft Academic Search

    Matook Saif Mokbel; Fumio Hashinaga

    The fresh green and yellow banana peel of, (Musa, cv. Cavendish) fruits were treated with 70% acetone, which were partitioned with chloroform (CHCl3) and ethyl acetate (EtOAc), sequentially. The antioxidant activities of the extracts were evaluated by using the thiocyanate method, ß-carotene bleaching method and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical elimination. While, antimicrobial activities of the extracts and isolated components were

  13. Effect of sowing dates and vernalization on Beta vulgaris L. cv. Univers C-leaf structure

    Microsoft Academic Search

    Mohammed M. Sakr; Omar A. Almaghrabi

    2011-01-01

    This research was conducted to study the effect of three different sowing dates (15th October, 15th November and 15th December) and two vernalization treatments (5°C and ?20°C) on leaf structure of Betavulgaris L. cv. Univers. The obtained data are summarized as follows:The maximum values of the most studied parameters; lower epidermis+spongy tissue thickness, midrib, mesophyll tissue, vascular bundle, collenchymatous tissue

  14. Teaching image processing and pattern recognition with the Intel OpenCV library

    NASA Astrophysics Data System (ADS)

    Koz?owski, Adam; Królak, Aleksandra

    2009-06-01

    In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image processing and pattern recognition with the most up-to-date solutions available. In the Institute of Electronics at the Technical University of Lodz we facilitate the teaching process in this subject with the OpenCV library, which is an open-source set of classes, functions and procedures that can be used in programming efficient and innovative algorithms for various purposes. The topics of student projects completed with the help of the OpenCV library range from automatic correction of image quality parameters or creation of panoramic images from video to pedestrian tracking in surveillance camera video sequences or head-movement-based mouse cursor control for the motorically impaired.

  15. Mechanisms of poron Adsorption on Metal Oxides. C.V. TONER, IV and D.l. SPARKS, Univ. of Delaware.

    E-print Network

    Sparks, Donald L.

    Mechanisms of poron Adsorption on Metal Oxides. C.V. TONER, IV· and D.l. SPARKS, Univ. of Delaware. It is widely known that boron reactions with oxides present in soils often regulate the amount of boron

  16. A New Platelet-Activating Factor Antagonist (CV6209) in Preservation of Heart and Lung for Transplantation

    Microsoft Academic Search

    Karim A. Qayumi; John C. English; Elizabeth J. E. Feeley; Ahmed Poostizadeh; Maryam Nikbakht-Sangari

    1997-01-01

    The objective of this experimental protocol was to evaluate theprotective effect of a new, potent platelet-activating factor (PAF)antagonist CV-6209 and the use of this compound in combination withallopurinol on ischemia-reperfusion injury in a swine model of heart-lungtransplantation. Forty-two swine were divided into three groups, with sevendonors and seven recipients in each. In group A, the PAF antagonist CV-6209was administered in

  17. Technology Assessment of Through-Silicon Via by Using $C$–$V$ and $C$–$t$ Measurements

    Microsoft Academic Search

    Guruprasad Katti; Michele Stucchi; Dimitrios Velenis; Sarasvathi Thangaraju; Kristin De Meyer; Wim Dehaene; Eric Beyne

    2011-01-01

    C-V characteristics of through-silicon vias (TSVs) manufactured in two different processing lines are compared to demonstrate the reproducibility of the TSV process module in terms of the minimum TSV depletion capacitance in the operating voltage region. TSV C-V and C-t measurements before and after thermocycling are employed for assessing the oxide liner and Ta barrier integrity of the TSV under

  18. Defective secretion of mucilage is the cellular basis for agravitropism in primary roots of Zea mays cv. Ageotropic

    NASA Technical Reports Server (NTRS)

    Miller, I.; Moore, R.

    1990-01-01

    Root caps of primary, secondary, and seminal roots of Z. mays cv. Kys secrete large amounts of mucilage and are in close contact with the root all along the root apex. These roots are strongly graviresponsive. Secondary and seminal roots of Z. mays cv. Ageotropic are also strongly graviresponsive. Similarly, their caps secrete mucilage and closely appress the root all along the root apex. However, primary roots of Z. mays cv. Ageotropic are non-responsive to gravity. Their caps secrete negligible amounts of mucilage and contact the root only at the extreme apex of the root along the calyptrogen. These roots become graviresponsive when their tips are coated with mucilage or mucilage-like materials. Peripheral cells of root caps of roots of Z. mays cv. Kys contain many dictyosomes associated with vesicles that migrate to and fuse with the plasmalemma. Root-cap cells of secondary and seminal (i.e. graviresponsive) roots of Z. mays cv. Ageotropic are similar to those of primary roots of Z. mays cv. Kys. However, root-cap cells of primary (i.e. non-graviresponsive) roots of Z. mays cv. Ageotropic have distended dictyosomal cisternae filled with an electron-dense, granular material. Large vesicles full of this material populate the cells and apparently do not fuse with the plasmalemma. Taken together, these results suggest that non-graviresponsiveness of primary roots of Z. mays cv. Ageotropic results from the lack of apoplastic continuity between the root and the periphery of the root cap. This is a result of negligible secretion of mucilage by cells along the edge of the root cap which, in turn, appears to be due to the malfunctioning of dictyosomes in these cells.

  19. Scheduling drip irrigation for fall tomatoes (Lycopersicon esculentum Mill. cv. Sunny) in the Lower Rio Grande Valley

    E-print Network

    Vargas Prieto, Alberto Miguel

    1985-01-01

    SCHEDULING DRIP IRRIGATION FOR FALL TOMATOES (, Lycapersi con escvientum Mill. cv. Sunny) IN THE LOWER RIO GRANDE VALLEY A Thesis ALBERTO MIGUEL VARGAS PRIETO Submitted to the Graduate College of Texas ALM University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE August 1985 Major Subject: Horticulture SCHEDULING DRIP IRRIGATION POR PALL TOMATOES (Iycopersi con esculentum Mill. cv. Sunny) IN TBE LOWER RIO GRANDE VALLEY A Thesis ALBERTO MIGUEL VARGAS PRIETO...

  20. Gryllotalpicola gen. nov., with descriptions of Gryllotalpicola koreensis sp. nov., Gryllotalpicola daejeonensis sp. nov. and Gryllotalpicola kribbensis sp. nov. from the gut of the African mole cricket, Gryllotalpa africana, and reclassification of Curtobacterium ginsengisoli as Gryllotalpicola ginsengisoli comb. nov.

    PubMed

    Kim, Hyangmi; Park, Doo-Sang; Oh, Hyun-Woo; Lee, Kang Hyun; Chung, Dong-Ho; Park, Ho-Yong; Park, Hee-Moon; Bae, Kyung Sook

    2012-10-01

    Strains RU-16(T), RU-28, RU-04(T) and PU-02(T) were isolated from the gut of the African mole cricket, Gryllotalpa africana. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strains belonged to the family Microbacteriaceae. All four strains were most closely related to Curtobacterium ginsengisoli DCY26(T) (below 97% 16S rRNA gene sequence similarity). These isolates were Gram-stain-positive, motile (by gliding), rod-shaped and exhibited ivory-coloured colonies. Their chemotaxonomic properties included MK-11 as the major respiratory quinone, ornithine as the cell-wall diamino acid, acetyl as the acyl type of the peptidoglycan, cyclohexyl-C(17:0) as the major fatty acid and phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, we propose a new genus in the family Microbacteriaceae, Gryllotalpicola gen. nov., with three novel species, Gryllotalpicola daejeonensis sp. nov. (type strain RU-04(T) ?=KCTC 13809(T) ?=JCM 17590(T)), Gryllotalpicola koreensis sp. nov. (type strain RU-16(T) ?=?KCTC 13810(T) ?=JCM 17591(T)) and Gryllotalpicola kribbensis sp. nov. (type strain PU-02(T) ?=KCTC 13808(T) ?=JCM 17593(T)). Gryllotalpicola koreensis is the type species of the genus. Additionally, we propose that Curtobacterium ginsengisoli should be reclassified in the genus as Gryllotalpicola ginsengisoli comb. nov. (type strain DCY26(T) ?=KCTC 13163(T) ?=?JCM 14773(T)). PMID:22140167

  1. Uso de granos secos con sol Ubles ( ddgs ) provenientes de la destilería del maíz en s Uplementos para v acas lactantes en pastoreo de estrella africana ( Cynodon nlemfluensis)1

    Microsoft Academic Search

    Sofía Macaya-Quirós; Augusto Rojas-Bourrillón

    Use of corn dry distillers grains plus solubles ( ddgs ) in supplements for lactating cows grazing african s tar grass (Cynodon nlemfluensis). A study was carried out with dry corn distillers grains plus solubles (DDGS), to evaluate the effect of including different levels of this ingredient in the diet of lactating dairy cows grazing African Star grass, on milk

  2. Dark inclusions in Allende, Leoville, and Vigarano - Evidence for nebular oxidation of CV3 constituents

    NASA Astrophysics Data System (ADS)

    Johnson, C. A.; Prinz, M.; Weisberg, M. K.; Clayton, R. N.; Mayeda, T. K.

    1990-03-01

    The origin and the history of dark inclusions (DIs) are investigated using petrologic, chemical, and oxygen isotopic data on ten DI samples from Allende, Leoville, and Vigarano. These data indicate that the DIs of the Leoville and Vigarano are closely similar to those of Allende. The inclusions appear to be fragments of CV3 parent bodies which were processed to different degrees prior to their incorporation as clasts into the Allende, Leoville, and Vigarano chondrites. The processing homogenized the olivine compositions, presumably through heating, and also involved oxygen exchange with O-16-poorer surroundings.

  3. Biological and phytochemical investigations of Dianthus barbatus cv. "China Doll" (Caryophyllaceae).

    PubMed

    Cordell, G A; Lyon, R L; Fong, H H; Benoit, P S; Farnsworth, N R

    1977-01-01

    From the aerial parts of Dianthus barbatus cv. "China Doll", two saponins (barbatosides A and B) were isolated and shown to have analgesic and anti-inflammatory activities. The aglycone of each saponin was identified as quillaic acid. The glycone of barbatoside A consisted of rhamnose, arabinose, fucose, xylose, galactose, glucose and one unidentified sugar; whereas the glycone of barbatoside B contained arabinose, fucose, xylose, mannose, galactose, glucose and three unidentified sugars. Astragalin, kaempferol-3-O-beta-D-sophoroside, D-pinitol and L-leucine were also isolated. PMID:895396

  4. Plant regeneration via somatic embryogenesis of Elymus sibiricus cv . ‘ chuancao No. 2 ’

    Microsoft Academic Search

    Daxu Li; Jie Zhang; Jian Zhao; Yi Zhang; Fei Chen; Jingqiu Zhu; Shujun Liu; Zhirong Yang

    2006-01-01

    The mature seeds, mesocotyls, and young leaf tips of Elymus  sibiricus L. cv. ‘chuancao No. 2’ were cultured on Murashige and Skoog (MS) medium supplemented with 5.0 mg\\/L 2,4-dichlorophenoxyacetic acid (2,4-d) and 0.05 mg\\/L kinetin in the dark at 26°C, the calluses were produced. The rate of callus regeneration depended on the explants\\u000a source and plant growth regulators. Plants regenerated from whitish-yellow-coloured

  5. Reproduction of Globodera rostochiensis on Transformed Roots of Solanum tuberosum cv. Desiree

    PubMed Central

    Kumar, A.; Forrest, J. M. S.

    1990-01-01

    Transformed roots of the susceptible potato Solanum tuberosum L. cv. Desiree were inoculated with second-stage juveniles (J2) of Globodera rostochiensis pathotype Rol. Adult males emerged after 3-4 weeks and matings with females occurred. After 8 weeks gentle pressure on the eggs of maturing females released the J2 which were viable. Because this technique enables the production of vigorously growing roots with numerous laterals, it may be suitable for obtaining a high yield of sterile potato cyst nematodes. PMID:19287735

  6. Influence of some herbicides on the reproduction of tobacco mosaic virus in Nicotiana tabacum cv. ‘Samsun’

    Microsoft Academic Search

    L. Šindelá?; Olga Makovcová

    1975-01-01

    The application of 10?3 g 1?1 of 2,4 dichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic acid, 3-amino-1,2,4-triazole and 6-chloro-2-ethylamino-4-isopropyl-amino-1,3,5-triazine\\u000a toNicotiana tabacum cv. ‘Samsun’ plants inoculated with tobacco mosaic virus results in an increase in the content of this virus in the tissues.\\u000a When whole plants are used, TMV content increases by 20% after herbicide application; when leaf dises are used, the amount\\u000a of the

  7. New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

    Microsoft Academic Search

    Budi Winarto; F. Rachmawati

    A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and\\u000a Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1–1.0 mg\\/l), ?-naphthalene\\u000a acetic acid (0.01–0.2 mg\\/l), thidiazuron (0.5–2.0 mg\\/l), 6-benzylaminopurine (0.5–1.0 mg\\/l), and kinetin (0.5–1.0 mg\\/l)] were\\u000a tested for their ability

  8. Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis

    Microsoft Academic Search

    Mya Thuzar; Apichart Vanavichit; Somvong Tragoonrung; Chatchawan Jantasuriyarat

    2011-01-01

    An efficient and rapid plant regeneration system through somatic embryogenesis was developed using 13-week-old zygotic embryos\\u000a of oil palm (Elaeis guineensis Jacq.) cv. ‘Tenera’. Zygotic embryos were cultured on MS and N6 media supplemented with 2.0 mg L?1 picloram, 2,4-D and dicamba. The highest embryogenic callus formation (32%) was observed on N6 medium with 2,4-D after 3 month\\u000a culture on callus induction

  9. The Parent Interview for Autism-Clinical Version (PIA-CV)A Measure of Behavioral Change for Young Children with Autism

    Microsoft Academic Search

    Wendy L. Stone; Elaine E. Coonrod; Stacie L. Pozdol; Lauren M. Turner

    2003-01-01

    The Parent Interview for Autism-Clinical Version (PIA-CV) was developed to measure autism symptom severity across a wide range of behavioral domains. Two studies were conducted to examine the psychometric properties of the PIA-CV for a sample of children under 3 years old. Results of study 1 revealed adequate internal consistency for nine of the 11 PIA-CV dimensions, as well as

  10. The Glycine max cv. Enrei Genome for Improvement of Japanese Soybean Cultivars

    PubMed Central

    Shimomura, Michihiko; Kanamori, Hiroyuki; Komatsu, Setsuko; Namiki, Nobukazu; Mukai, Yoshiyuki; Kurita, Kanako; Kamatsuki, Kaori; Ikawa, Hiroshi; Yano, Ryoichi; Ishimoto, Masao; Kaga, Akito; Katayose, Yuichi

    2015-01-01

    We elucidated the genome sequence of Glycine max cv. Enrei to provide a reference for characterization of Japanese domestic soybean cultivars. The whole genome sequence obtained using a next-generation sequencer was used for reference mapping into the current genome assembly of G. max cv. Williams 82 obtained by the Soybean Genome Sequencing Consortium in the USA. After sequencing and assembling the whole genome shotgun reads, we obtained a data set with about 928?Mbs total bases and 60,838 gene models. Phylogenetic analysis provided glimpses into the ancestral relationships of both cultivars and their divergence from the complex that include the wild relatives of soybean. The gene models were analyzed in relation to traits associated with anthocyanin and flavonoid biosynthesis and an overall profile of the proteome. The sequence data are made available in DAIZUbase in order to provide a comprehensive informatics resource for comparative genomics of a wide range of soybean cultivars in Japan and a reference tool for improvement of soybean cultivars worldwide. PMID:26199933

  11. Heterogeneous compute in computer vision: OpenCL in OpenCV

    NASA Astrophysics Data System (ADS)

    Gasparakis, Harris

    2014-02-01

    We explore the relevance of Heterogeneous System Architecture (HSA) in Computer Vision, both as a long term vision, and as a near term emerging reality via the recently ratified OpenCL 2.0 Khronos standard. After a brief review of OpenCL 1.2 and 2.0, including HSA features such as Shared Virtual Memory (SVM) and platform atomics, we identify what genres of Computer Vision workloads stand to benefit by leveraging those features, and we suggest a new mental framework that replaces GPU compute with hybrid HSA APU compute. As a case in point, we discuss, in some detail, popular object recognition algorithms (part-based models), emphasizing the interplay and concurrent collaboration between the GPU and CPU. We conclude by describing how OpenCL has been incorporated in OpenCV, a popular open source computer vision library, emphasizing recent work on the Transparent API, to appear in OpenCV 3.0, which unifies the native CPU and OpenCL execution paths under a single API, allowing the same code to execute either on CPU or on a OpenCL enabled device, without even recompiling.

  12. Multi-camera calibration based on openCV and multi-view registration

    NASA Astrophysics Data System (ADS)

    Deng, Xiao-ming; Wan, Xiong; Zhang, Zhi-min; Leng, Bi-yan; Lou, Ning-ning; He, Shuai

    2010-10-01

    For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the corresponding relationship between each camera view, the computation of the rotation matrix and translation matrix is formulated as a constrained optimization problem. According to the Kuhn-Tucker theorem and the properties on the derivative of the matrix-valued function, the formulae of rotation matrix and translation matrix are deduced by using singular value decomposition algorithm. Afterwards an iterative method is utilized to get the entire coordinate transformation of pair-wise views, thus the precise multi-view registration can be conveniently achieved and then can get the relative positions in them(the camera outside the parameters).Experimental results show that the method is practical in multi-camera calibration .

  13. Electroacupuncture Stimulation at CV12 Inhibits Gastric Motility via TRPV1 Receptor

    PubMed Central

    Yu, Zhi; Cao, Xin; Xia, Youbing; Ren, Binbin; Feng, Hong; Wang, Yali; Jiang, Jingfeng; Xu, Bin

    2013-01-01

    Gastric dysmotility is one of the major pathophysiological factors in functional gastrointestinal disorders. Acupuncture, as one of the alternative approaches, is efficacious in the treatment of gastrointestinal motility disorders; however, the mechanism underlying its action is unclear. In the present study, we used both capsazepine, a TRPV1 antagonist, and TRPV1 knockout mice. Animals were divided into wild-type group (WT), capsazepine injection group (CZP, 0.5?mg/kg, i.p.), and TRPV1 knockout mice group (TRPV1?/?). Each of these three groups was divided into three subgroups, which were subjected to EA stimulation at acupoint Zhongwan (CV12) at a different intensity (1, 2, or 4?mA). We demonstrated that electroacupuncture at Zhongwan (CV12) markedly inhibited gastric motility at 2 and 4?mA in an intensity-dependent manner in wild-type mice. The inhibitory effect was also observed in capsazepine-injected and TRPV1?/? mice but was no longer intensity dependent, indicating that TRPV1 is partially involved in the electroacupuncture-mediated modulation of gastric motility. PMID:24106521

  14. Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO.

    PubMed

    Albert, Andreas; Sareedenchai, Vipaporn; Heller, Werner; Seidlitz, Harald K; Zidorn, Christian

    2009-05-01

    Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity of the tissues involved. Factors causing these variations were investigated by climate chamber (CC) experiments focusing on temperature and ultraviolet (UV)-B radiation. Plants of A. montana L. cv. ARBO were grown in CCs under realistic climatic and radiation regimes. Key factors temperature and UV-B radiation were altered between different groups of plants. Subsequently, flowering heads were analyzed by HPLC for their contents of flavonoids and caffeic acid derivatives. Surprisingly, increased UV-B radiation did not trigger any change in phenolic metabolites in Arnica. In contrast, a pronounced increase in the ratio of B-ring ortho-diphenolic (quercetin) compared to B-ring monophenolic (kaempferol) flavonols resulted from a decrease in temperature by 5 degrees C in the applied climate regime. In conclusion, enhanced UV-B radiation is probably not the key factor triggering shifts in the phenolic composition in Arnica grown at higher altitudes but rather temperature, which decreases with altitude. PMID:19194724

  15. Mechanisms of HIV-1 subtype C resistance to GRFT, CV-N and SVN

    PubMed Central

    Alexandre, Kabamba B.; Moore, Penny L.; Nonyane, Molati; Gray, Elin S.; Ranchobe, Nthabeleng; Chakauya, Ereck; McMahon, James B.; O’Keefe, Barry R.; Chikwamba, Rachel; Morris, Lynn

    2013-01-01

    We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. Four primary HIV-1 strains cultured under escalating concentrations of these lectins became increasingly resistant tolerating 2 to 12 times their 50% inhibitory concentrations. Sequence analysis of gp120 showed that most had deletions of 1 to 5 mannose-rich glycans. Glycosylation sites at positions 230, 234, 241, 289 located in the C2 region and 339, 392 and 448 in the C3-C4 region were affected. Furthermore, deletions and insertions of up to 5 amino acids in the V4 region were observed in 3 of the 4 isolates. These data suggest that loss of glycosylation sites on gp120 as well as rearrangement of glycans in V4 are mechanisms involved in HIV-1 subtype C escape from GRFT, CV-N and SVN. PMID:24074568

  16. In Situ Measurement of Oxygen Isotope Compositions of Magnetite in the Allende CV3 Chondrite

    NASA Astrophysics Data System (ADS)

    Choi, B.-G.; Coath, C. D.; Leshin, L.; Wang, J.; McKeegan, K. D.; Wasson, J. T.

    1995-09-01

    Magnetite is relatively abundant in the oxidized CV chondrites. This study was undertaken to examine possible origins in nebular or in parent-body processes. Magnetite in Allende has several distinct characteristics compared to that of the other CV chondrites. Unlike Mokoia and Vigarano, magnetite in Allende is almost exclusively found in chondrules, where it typically forms spherical nodules often associated with metal and/or sulfides. In addition, the Ni contents of metal in most CV chondrites seldom exceed 50 wt%, while in Allende nearly all of metal grains are Ni rich (64-71 wt% Ni [e.g., 1]), which we designate awaruite even though the Ni range is below that of awaruite. Rare kamacite grains are present in chondrules [2]. A few rare Allende nodules have euhedral to subhedral awaruite grains [e.g., 3]. Nodules of magnetite + sulfides, magnetite + metal, and metal + sulfide exhibit different textures implying that magnetite grains are not simply pseudomorphs of preexisting metal grains. Tiny magnetite grains (< 1 micrometer) are found in Ni-rich metal ; in turn, the host metal is surrounded by magnetite. The textural evidence is not sufficient to distinguish between a high temperature and a low temperature origin for the magnetite. Six magnetite nodules, which had relatively clean areas of ~ 25 micrometers diameter, from 4 Allende PO chondrules were selected for in situ oxygen isotope study using the UCLA Cameca ims 1270, a high resolution - high sensitivity ion microprobe. A ~0.3 nA primary Cs+ beam was defocused to a 20-25 micrometers spot and a field aperture inserted into the secondary ion beam to restrict the analyzed area to the central 10-12 micrometers of each nodule. Negative ions were collected at a mass resolving power of ~6500, sufficient to eliminate hydride interferences. A normal incidence electron gun was employed to compensate possible sample charging. To monitor the instrumental mass fractionation (-11.2+/-0.5 per mil/amu) analyses of terrestrial magnetite LP204a [4] were made interspersed amongst the chondrule analyses. Chondrule data corrected for mass fractionation and normalized to the SMOW scale are shown in Figure 1. Oxygen isotope ratios from inclusion and crack free regions in four magnetite nodules are tightly clustered below the terrestrial mass fractionation (TF) line and are distinct from oxygen isotope fields observed in magnetite from CI and Essebi chondrites [5] and from isolated grains in CI meteorites [6]. The data plot near the ^(16)O mixing line of refractory minerals in Allende (CCAM), but the spread in the ratios is too low relative to the precision of the measurements to define any linear trend for these samples. In 2 chondrules, magnetite nodules with cracks or Ni-Fe metal have very different oxygen compositions; they plot near the TF line at low delta^(18)O (-10 per mil). At this time, it is not clear if this difference could be due to a sputtering artifact, or if it reflects alteration or inclusion of another phase in these "magnetite" nodules. The question of the origin of CV magnetite has not yet been resolved. Data on additional samples including other CV chondrites will help clarify this point. References: [1] McSween H. Y. Jr. (1977) GCA, 41, 1777-1790. [2] Fuchs L. H. and Olsen E. (1973) EPSL, 18, 379-384. [3] Rubin A. E. (1991) Am. Mineral., 76, 1356-1362. [4] Valley J. W. and Graham G. M. (1991) Contrib. Mineral. Petrol., 109, 38-52. [5] Rowe M. W. et al. (1994) GCA, 58, 5341-5347. [6] Hyman M. et al. (1991) Meteoritics, 26, 350.

  17. Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves.

    PubMed

    Jung, Kwonil; Scheuer, Kelly A; Zhang, Zhenwen; Wang, Qiuhong; Saif, Linda J

    2014-01-10

    The pathogenesis of GIII.2 bovine norovirus (BoNoV) is not well understood. Our study demonstrated persisting diarrhea and prolonged fecal shedding, but with a lack of significant intestinal lesions in gnotobiotic (Gn) calves infected with GIII.2 BoNoV, CV186-OH/00/US strain. Nine 4 to 7-day-old Angus/Jersey crossbred Gn calves were orally inoculated with 10.0-11.9 log10 genomic equivalents (GE)/calf of CV186-OH (n=7) or mock (n=2). Calves were euthanized at post-inoculation day (PID) 1 (n=1) when moderate to severe lethargy was observed and at PIDs 2-6 (n=4) after lethargy had subsided. Two calves were kept longer term (until PID 30) for monitoring fecal shedding patterns by TaqMan real-time RT-PCR (qRT-PCR). Most infected calves exhibited two clinical signs: (i) acute but persisting diarrhea and (ii) acute moderate to severe lethargy. The two infected calves, followed longer-term, had prolonged fecal viral RNA shedding [peak average titer of 11.8 (± 0.2) log10GE/ml] at least until PID 20. By qRT-PCR, 5 infected calves had low viral RNA titers in serum, ranging from 4.0 to 5.8 log10GE/ml, at PIDs 1-5, but not (<2.7 log10GE/ml) at PIDs 6-30. The latter observation coincided with the presence of serum IgG antibody to BoNoV at PIDs 8-30. Collectively, the GIII.2 BoNoV strain CV186-OH induced only mild enteropathogenicity, evident by the lack of significant intestinal lesions, but it led to persisting mild diarrhea and prolonged fecal virus shedding in Gn calves. The prolonged fecal shedding of GIII.2 BoNoV might partially explain how this virus is maintained as endemic infections in cattle. PMID:24309403

  18. EFECTO DEL ENVASE DE CARTON CORRUGADO Y EMBALAJE EN LA CONSERVACION DE LA CALIDAD DE FRUTA DE AGUACATE CV HASS EFFECT OF SINGLEWALL CORRUGATED FIBREBOARD CONTAINER AND PACKAGING FOR TO THE CONSERVATION OF AVOCADO FRUIT QUALITY CV HASS

    Microsoft Academic Search

    Luis López López; Jacques F. Cajuste Bontemps

    Avocado fruits (Persea americana Mill.) cv Hass were harvested at physiological maturity and it was packed in four different types of carton boxes of 4 kg capacity for to evaluate the postharvest effect to environmental trials like under storage during 20 days using one temperature of 5±1º C, at environmental trials were not differences, meanwhile under storage we founded the

  19. Use of nootkatone as a senescence indicator for Rouge La Toma Cv. grapefruit (Citrus paradisi Macf.).

    PubMed

    Biolatto, Andrea; Sancho, Ana M; Cantet, Rodolfo J C; Güemes, Daniel R; Pensel, Norma A

    2002-08-14

    The objective of this research was to study the usefulness of nootkatone as a senescence indicator for Rouge La Toma cv. grapefruit (Citrus paradisi Macf.), simulating different treatments that included the normal postharvest handling of citrus fruits: temperature conditioning, cold storage, shipment periods to overseas markets such as Japan and the U.S., marketing conditions, and storage at nonchilling temperature (control treatments). The highest nootkatone levels, determined by GLC-MS analyses, were detected in fruits subjected to control treatments. No significant differences were observed in nootkatone levels between treatments either with or without temperature conditioning prior to the start of the cold storage. Levels of nootkatone increased throughout time for all assayed treatments. The linear regressions of nootkatone levels showed correlation coefficients of 0.80 and 0.83 with storage time (29 and 42 days, respectively). Therefore, nootkatone appears to be a good indicator of senescence for Rouge La Toma grapefruit. PMID:12166965

  20. Inducing gravitropic curvature of primary roots of Zea mays cv Ageotropic

    NASA Technical Reports Server (NTRS)

    Moore, R.; Evans, M. L.; Fondren, W. M.

    1990-01-01

    Primary roots of the mutant 'Ageotropic' cultivar of Zea mays are nonresponsive to gravity. Their root caps secrete little or no mucilage and touch the root only at the extreme apex. A gap separates the cap and root at the periphery of the cap. Applying mucilage from normal roots or substances with a consistency similar to that of mucilage to tips of mutant roots causes these roots to become strongly graviresponsive. Gravicurvature stops when these substances are removed. Caps of some mutants secrete small amounts of mucilage and are graviresponsive. These results indicate that (a) the lack of graviresponsiveness in the mutant results from disrupting the transport pathway between the cap and root, (b) movement of the growth-modifying signal from the cap to the root occurs via an apoplastic pathway, and (c) mucilage is necessary for normal communication between the root cap and root in Zea mays cv Ageotropic.

  1. Influence of different drying methods on carotenoids and capsaicinoids of paprika (Cv., Jalapeno).

    PubMed

    Topuz, Ayhan; Dincer, Cuneyt; Ozdemir, Kubra Sultan; Feng, Hao; Kushad, Mosbah

    2011-12-01

    Influence of Refractance Window™ Drying (RWD), a novel contact drying method, on carotenoids, capsaicinoids, Retinol Activity Equivalent (RAE) and Scoville Heat Unit (SHU) of paprika (Cv., Jalapeno) was investigated in comparison with freeze drying (FD), oven drying (OD), and natural convective drying (NCD) methods. Eight carotenoids (capsanthin, capsorubin, capsolutein, ?-carotene, ?-cryptoxanthin, mutatoxanthin, violaxanthin and zeaxanthin) and five capsaicinoid analogues (capsaicin, dihydrocapsaicin, homodihydrocapsaicin, isodihydrocapsaicin, nordihydrocapsaicin) were identified in paprika. All these components were significantly (P<0.05) decreased by the RWD, FD and OD methods. However, due to ongoing synthesis, the NCD method resulted in higher carotenoids, except violaxanthin and mutatoxanthin, and capsaicinoids content than those of the others, even puree. Mutatoxanthin, naturally occurring pigment in red pepper, could only be detected in FD paprika. The highest RAE and SHU values, which were derived from the data of carotenoids and capsaicinoids, respectively, were also determined in NCD paprika. PMID:25212310

  2. The fractionation of noble gases in diamonds of CV3 Efremovka chondrite

    NASA Technical Reports Server (NTRS)

    Fisenko, A. V.; Verchovsky, A. B.; Semjonova, L. F.; Shukolyukov, Yu. A.

    1993-01-01

    It was shown that in diamonds of Efremovka CV3 the noble gases with normal isotopic compositions are fractionated in different degree while the correlation of isotopic anomalous components is nearly constant. Some data for noble gases in DE-4 sample of Efremovka chondrite are considered. In contrast to DE-2 sample the DE-4 was treated except conc. HClO4, 220 C in addition with mixture of conc. H2SO4+H3PO4 (1:1), 220 C, twice. Noble gases analysis were performed in Germany at Max Plank Institute fur Chemie. Noble gases were released by oxidation of samples at stepped heating from 420 C to 810 C and by pyrolysis at 580, 590, and 680 C.

  3. Defect Detection in Superconducting Radiofrequency Cavity Surface Using C + + and OpenCV

    NASA Astrophysics Data System (ADS)

    Oswald, Samantha; Thomas Jefferson National Accelerator Facility Collaboration

    2014-03-01

    Thomas Jefferson National Accelerator Facility (TJNAF) uses superconducting radiofrequency (SRF) cavities to accelerate an electron beam. If theses cavities have a small particle or defect, it can degrade the performance of the cavity. The problem at hand is inspecting the cavity for defects, little bubbles of niobium on the surface of the cavity. Thousands of pictures have to be taken of a single cavity and then looked through to see how many defects were found. A C + + program with Open Source Computer Vision (OpenCV) was constructed to reduce the number of hours searching through the images and finds all the defects. Using this code, the SRF group is now able to use the code to identify defects in on-going tests of SRF cavities. Real time detection is the next step so that instead of taking pictures when looking at the cavity, the camera will detect all the defects.

  4. Bioresorbable adhesion barrier for reducing the severity of postoperative cardiac adhesions: Focus on REPEL-CV®

    PubMed Central

    Haensig, Martin; Mohr, Friedrich Wilhelm; Rastan, Ardawan Julian

    2011-01-01

    Treatment of a number of congenital heart defects often necessitates staged surgical intervention. In addition, substantial improvements in postoperative cardiac care and more liberal use of biological valve substitutes have resulted in many adult patients surviving to become potential candidates for reoperations to repair or replace valves or to undergo additional revascularization procedures. In all these scenarios, surgeons are confronted with cardiac adhesions, leading to an increased surgical risk. Thus, bioresorbable adhesion barriers had become of increasing interest because they are easy to use, and safe and effective. This review focuses on the mechanisms by which REPEL-CV® prevents adhesive processes, as well as the development, design, and materials used, and also summarizes efficacy studies, clinical data, safety, and current role in therapy. PMID:22915926

  5. Changes in color and phenolic compounds during the raisining of grape cv. Pedro Ximenez.

    PubMed

    Serratosa, María P; Lopez-Toledano, Azahara; Merida, Julieta; Medina, Manuel

    2008-04-23

    Changes in color parameters and phenolic compounds during the sun-drying grape raisining of cv. Pedro Ximenez to obtain sweet wines are studied. Browning increases during the process as a result of the contribution to a greater extent of the low and medium molecular size polymers than the high molecular size polymers. Raisining decreases hue and lightness and increases chroma, all measured as CIELab parameters, indicating a color change to dark reddish hues that is also preferentially due to low and medium molecular size polymers. Most of the phenols studied increase in concentration during raisining, essentially through the concentration effect resulting from the loss of water in the grapes. The concentration changes, however, are comparatively small for hydroxycinnamic esters and flavan-3-ol derivatives, suggesting that these phenolic fractions undergo predominantly oxidative degradation reactions by enzymatic pathways, contributing strongly to the browning of grapes. PMID:18345634

  6. Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”

    PubMed Central

    Sun, Jian; Wang, Hexiang; Ng, Tzi Bun

    2010-01-01

    A purification protocol that comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75 was complied to isolate two trypsin inhibitors from Phaseolus vulgaris cv “White Cloud Bean”. Both trypsin inhibitors exhibited a molecular mass of 16?kDa and reduced the activity of trypsin with an IC50 value of about 0.6??M. Dithiothreitol attenuated the trypsin inhibitory activity, signifying that an intact disulfide bond is indispensable to the activity. [Methyl-3H] thymidine incorporation by leukemia L1210 cells was inhibited with an IC50 value of 28.8??M and 21.5??M, respectively. They were lacking in activity toward lymphoma MBL2 cells and inhibitory effect on HIV-1 reverse transcriptase and fungal growth when tested up to 100??M. PMID:20617140

  7. Overseas trip report, CV 990 underflight mission. [Norwegian Sea, Greenland ice sheet, and Alaska

    NASA Technical Reports Server (NTRS)

    Gloersen, P.; Crawford, J.; Hardis, L.

    1980-01-01

    The scanning microwave radiometer-7 simulator, the ocean temperature scanner, and an imaging scatterometer/altimeter operating at 14 GHz were carried onboard the NASA CV-990 over open oceans, sea ice, and continental ice sheets to gather surface truth information. Data flights were conducted over the Norwegian Sea to map the ocean polar front south and west of Bear Island and to transect several Nimbus-7 footprints in a rectangular pattern parallel to the northern shoreline of Norway. Additional flights were conducted to obtain correlative data on the cryosphere parameters and characteristics of the Greenland ice sheet, and study the frozen lakes near Barrow. The weather conditions and flight path way points for each of the nineteen flights are presented in tables and maps.

  8. [Models for estimating foliar Fe and Mn Concentration of Armeniaca vulgaris cv. Luntaibaixing using spectral reflectance].

    PubMed

    Hu, Zhen-Zhu; Pan, Cun-De; Wang, Shi-Wei; Guo, Zhi-Chao; Wang, Qing-Tao; Ding, Fan; Li, Yuan

    2014-09-01

    Aimed at providing technology for a rapid nutrition diagnosis system of micronutrients in Armeniaca vulgaris cv. Luntaibaixing, we established an element concentration estimation model for its foliar ferrum (Fe) and manganese (Mn) concentration based on spectrum analysis. The foliar spectrum reflectance at various phenological periods of fruit development under different soil fertility conditions was measured by Unispec-SC spectrometer. By analyzing the correlation of foliar Fe, Mn concentration at various phenological periods of fruit development, the spectrum reflectance R? and its first-order differential f' (R?), we filtered out its sensitive bands. And we established an element concentration estimation model for its foliar Fe and Mn at various phenological periods of fruit development with the linear regression model. The results showed that the spectral sensitive bands of foliar Fe in fruit setting period were 873 and 874 nm, 375 and 437 nm in fruit core-hardening period, 836 and 837 nm in maturity period and 325 and 1 054 nm in post-harvest period. However, the spectral sensitive bands of Mn were 913 and 1 129 nm, 425 and 970 nm, 390 and 466 nm, 423 and 424 nm, respectively. The Fe and Mn concentration of A. vulgaris cv. Luntaibaixing leaves were the most relevant to the first-order differential f' (RD) of its spectrum reflectance, whose linear spectrum estimation model fitting degree was the highest and reached to a significant or highly significant level. It showed that the spectral sensitive bands of Fe and Mn element varied with different phenological periods of fruit development. The spectrum estimation models for its foliar Fe and Mn concentration could be established with linear model according to its first-order differential f' (R?). PMID:25532350

  9. Economic valuation of the Seto Inland Sea by using an Internet CV survey.

    PubMed

    Tsuge, Takahiro; Washida, Toyoaki

    2003-01-01

    We estimate the economic value of the natural environment damaged in the Seto Inland Sea after the introduction of the Law on Temporary Measures for the Environmental Conservation of the Seto Inland Sea (Setouchi Law) and the value of the natural environment that survived, using a Contingent Valuation (CV) survey on an Internet web site. The CV survey contains three plans. Plan 1 is to restore 4 ha of reclaimed land. By estimating the Willingness To Pay (WTP) for plan 1, we can appraise the value of the natural environment that was damaged as a result of the original reclamation. Plan 2 is to transplant Zostera (eel-grass) into an area of 10 ha offshore. Plan 3 is to preserve the shore area, a natural habitat for rare animal species, under the National Trust Program. From the WTP for plans 2 and 3, we can estimate the value of the shore area and the areas a little farther offshore. The value of the natural environment damaged in the Seto Inland Sea as a result of reclaiming projects after the introduction of the Setouchi Law and the value of the existing natural environment of the Seto Inland Sea from the WTP for the plans were estimated to about 172 trillion yen (1.46 trillion dollars) and about 424 trillion yen (3.60 trillion dollars), respectively. The results indicate that in the 25 years since the introduction of the Setouchi Law, we have degraded every year about 6.88 trillion yen (58.5 billion dollars) worth of the natural environment by reclaiming. Some seaweed farms and natural shore areas, natural habitats to rare marine life-forms like the horseshoe crab and the fiddler crab have survived, but their value amounts to about 80% of Japan's GDP. PMID:12787625

  10. PII S0016-7037(99)00046-0 Origin of compact type A refractory inclusions from CV3 carbonaceous chondrites

    E-print Network

    Grossman, Lawrence

    PII S0016-7037(99)00046-0 Origin of compact type A refractory inclusions from CV3 carbonaceous types of coarse-grained refractory inclusions found in carbonaceous chondrites. They have not been of the two main types of coarse- grained, refractory inclusions recognized by Grossman (1975) in a study

  11. Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pera

    Microsoft Academic Search

    Rubia P. Purcino; Camilo Lazaro Medina; Daniel Martins de Souza; Flavia Vischi Winck; Eduardo Caruso Machado; JoseCamilo Novello; Marcos Antonio Machado; Paulo Mazzafera

    2007-01-01

    Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pera' (C. sinensis L.

  12. Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg

    E-print Network

    Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg GRECO Cvo UNI OL/ Nds.PV-Symp. 06-2009 semiconductor physics / quantum solar energy conversion;Semiconductor Physics and Quantum Solar Energy Conversion Semiconductor Physics and Quantum Solar Energy

  13. Effect of Cutting Heights on Productivity and Quality of King Napier Grass (Pennisetum purpureum cv. King Grass) under Irrigation

    Microsoft Academic Search

    Sumran Wijitphan; Pornchai Lorwilai; Chutipong Arkaseang

    2009-01-01

    2 Abstract: An experiment to study the effect of cutting heights on yield and nutritive values of King napier grass (Pennisetum purpureum cv. King grass) was conducted on sandy loam, Korat soil series (Oxic Paleustults) under irrigation during June 2006 to November 2007 at Khon Kaen Animal Nutrition Development Research Center, Thailand. Four treatments of cutting height at 0 (T1),

  14. Aerial Growth and Dry Matter Production of Potato (Solanum tuberosum L.) cv. Desiree in Relation to Phosphorus Application

    Microsoft Academic Search

    HAKOOMAT ALI; MUHAMMAD AKBAR ANJUM

    The growth of potato cv. Desiree in relation to phosphorus fertilizer application was studied at the Morfa Mawr Field Research Station, University of Wales, Aberystwyth, UK during 1995. The sprouted seed tubers were planted on ridges prepared 70 cm apart, at a distance of 28 cm. Six different levels of phosphorus fertilizer (i.e. 0, 50, 100, 200, 300 and 400

  15. Water Deficit Effect on Ratio of Seed to Berry Fresh Weight and Berry Weight Uniformity in Winegrape cv. Merlot

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Field-grown grapevines cv. Merlot (Vitis vinifera L.) were differentially irrigated in a randomized block design during two growing seasons to maintain a high or low level of vine water stress between fruit set and harvest. Detached berries from clusters harvested at maturity were individually weig...

  16. Variation in structure and plant regeneration of Agrobacterium rhizogenes transformed and control roots of the potato cv. Bintje

    Microsoft Academic Search

    M. P. Ottaviani; J. H. N. Schel; Ch. H. Hänisch ten Cate

    1990-01-01

    Agrobacterium rhizogenes transformed and control roots of the tetraploid potato cv. Bintje were compared. Transformed roots were obtained after infection by A. rhizogenes 15834 or 1855. Both in leaf and stem segments, more roots were formed at the basal side of the segments, indicative for a polarity in root formation. As compared to control roots the transformed roots are characterized

  17. Abscisic acid controls embryo growth potential and endosperm cap weakening during coffee ( Coffea arabica cv. Rubi) seed germination

    Microsoft Academic Search

    E. A. Amaral da Silva; Peter E. Toorop; Adriaan C. van Aelst; Henk W. M. Hilhorst

    2004-01-01

    The mechanism and regulation of coffee seed germination were studied in Coffea arabica L. cv. Rubi. The coffee embryo grew inside the endosperm prior to radicle protrusion and abscisic acid (ABA) inhibited the increase in its pressure potential. There were two steps of endosperm cap weakening. An increase in cellulase activity coincided with the first step and an increase in

  18. Confirmation By QTL mapping Of The Malus Robusta (Cv. Robusta 5) derived powdery mildew resistance gene Pl1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Geneva® apple rootstock breeding program has made extensive use of Malus robusta cv. Robusta 5 as a source of resistance to fire blight. Robusta 5 has also been used as the source of powdery mildew resistance by other breeding programs and a single locus Pl1 has been associated with this resist...

  19. Microbiological and biochemical profile of cv. Conservolea naturally black olives during controlled fermentation with selected strains of lactic acid bacteria

    Microsoft Academic Search

    Efstathios Z. Panagou; Ulrich Schillinger; Charles M. A. P. Franz; George-John E. Nychas

    2008-01-01

    The effect of controlled fermentation processes on the microbial association and biochemical profile of cv. Conservolea naturally black olives processed by the traditional anaerobic method was studied. The different treatments included (a) inoculation with a commercial starter culture of Lactobacillus pentosus, (b) inoculation with a strain of Lactobacillus plantarum isolated from a fermented cassava product and (c) uninoculated spontaneous process.

  20. CV Diana Krull I was born 1930 in Tallinn, Estonia and came to Sweden after having completed my

    E-print Network

    , German, Estonian, General Linguistics and Phonetics at Stockholm University. While working part timeCV Diana Krull I was born 1930 in Tallinn, Estonia and came to Sweden after having completed my and in the Swedish radio. At the same time, I worked as a music teacher. In the 1970-ies, I studied Spanish, English

  1. VALIDACIÓN CLINICA DE UN GUANTE CON INDICADOR DE PH (VAGITEST®) PARA LA APROXIMACIÓN DIAGNÓSTICA DE LAS INFECCIONES VAGINALES CLINICAL VALIDATION OF A GLOVE WITH PH INDICATOR (VAGITEST®) IN DIAGNOSIS APPROACH OF VAGINAL INFECTION

    Microsoft Academic Search

    Miguel Tilli; Adrián Orsini; María Marta Alvarez; Marisa Almuzara; Elena Gallardo; Juan O Mormandi

    o de agosto de 2006 e 30 de abril de 2007. Todas as pacientes incluídas utilizaram o Vagitest® antes de realizar a rotina microbiológica para infecções CV, que incluiu: exame clínico com especuloscopia, determinação do pH vaginal mediante o uso de fitas reativas tra- dicionais, teste de aminas (OHK 10%) e exame microscópico a fresco e após a coloração de

  2. ¿Crecer para exportar o exportar para crecer?

    Microsoft Academic Search

    Julio César Alonso; Carlos Patiño

    2005-01-01

    Este documento investiga la validez de la hipótesis de crecimiento económico basado en el incremento de las exportaciones empleando datos anuales para el Valle del Cauca durante el periodo 1960 – 2000. No se encuentra evidencia suficiente para validar la relación causal que supone la hipótesis de crecimiento económico basado en exportaciones. Se emplea un modelo VAR que incluye la

  3. Microtextures of Phyllosilicates in the Mokoia CV Chondrite: Evidence for Aqueous Alteration Prior to Consolidation

    NASA Astrophysics Data System (ADS)

    Tomeoka, K.

    1993-07-01

    The CV and CO chondrites were less affected by aqueous alteration than the CI and CM chondrites; thus they may serve as important indicators of the earliest stages of carbonaceous chondrite formation. Mokoia is one of the rare CV chondrites that contains considerable amounts of phyllosilicates [1,2]. They occur in CAIs, some chondrules, and matrix; they also occur abundantly in some chondrule rims. Tomeoka and Buseck [2] showed that the phyllosilicates in Mokoia are saponite and Na-rich phlogopite and were formed by aqueous alteration of olivine, pyroxene, anorthite, and glass. However, the mineralogy and occurrence of phyllosilicates in Mokoia are different from those in CI and CM chondrites, suggesting that the alteration of Mokoia occurred in a condition distinct from those for CI and CM chondrites. Most phyllosilicates in CI and CM chondrites probably resulted from alteration of olivine and pyroxene by the activity of liquid water on the meteorite parent bodies (e.g., [3]). Major questions are (1) What were the conditions responsible for the alteration in Mokoia? (2) Were these conditions different from those experienced by the CI and CM chondrites? (3) Where did the aqueous alteration in Mokoia occur? To address these questions, I performed extensive observations of the Mokoia CV chondrite by using a scanning electron microscope equipped with an EDS analysis system. The Mokoia chondrite is a breccia composed of submillimeter to millimeter clasts. Its matrix consists largely of fine grains (<0.1 to 10 micrometers in diameter) of Fe-rich olivine. Phyllosilicates occur in narrow interstices (<1 micrometer) between the olivine grains. The abundance of phyllosilicates in matrix differs within a clast and among clasts. Some clasts that are rich in phyllosilicates are adjacent to phyllosilicate-poor or -free clasts; the evidence suggests that aqueous alteration of the matrix preceded some of the brecciation events that produced the clasts from earlier matrix. Phyllosilicates are much more abundant in some chondrules and CAIs than in matrix in Mokoia; in some chondrules, phyllosilicates extend over an area of 100 x 100 micrometers. Chondrules and inclusions that were altered in various degrees occur together with those that remain unaltered within the same clasts Because of small sizes, olivines in the Mokoia matrix are more permeable to fluids and thus more reactive to aqueous alteration than the coarser olivines and pyroxenes in chondrules. Therefore, if alteration.of chondrules occurred in situ in the parent body (after consolidation), the matrix olivines should have been altered preferentially to those in chondrules, which is just the opposite of what I observe. These observations suggest that some chondrules and inclusions experienced a distinct environment in terms of hydration from that of the matrix materials. Mineralogy itself does not provide precise constraints on whether the alteration occurred via a solar nebular gas or aqueous solutions. However, based on the present observations, it is likely that some chondrules, inclusions, and portions of matrix in Mokoia have experienced aqueous alteration prior to consolidation into the present configuration. References: [1] Cohen R. E. et al. (1983) GCA, 47, 1739-1757. [2] Tomeoka K. and Buseck P. R. (1990) GCA, 54, 1745-1754. [3] Tomeoka K. (1990) Nature, 345, 138-140.

  4. Spectroscopy of Pre-CV Candidates in the Open Cluster M 67

    NASA Astrophysics Data System (ADS)

    Balaguer-Núñez, L.; Galadí-Enríquez, D.; Jordi, C.; Sánchez, S.

    The systematic study of selected open clusters by our team has lead to the production of the best set of Strömgren photometry ever obtained of the old open cluster M 67. Its analysis has shown a previously unknown clump of more than 50 stars in the HR diagram, located below the cluster MS. The spatial distribution of these stars indicates that most of them could be cluster members. Two alternative hypothesis would explain their photometry: (1) if members, they would be binary systems composed by a white dwarf and a red dwarf, i.e. pre-cataclysmic variable systems; (2) if non-members, they would constitute a stream of G-type stars placed behind the cluster. Medium dispersion spectra taken by our team using the PMAS/PPAK at 3.5 m telescope in Calar Alto1 will show the composite or single nature of the objects, and will allow to deblend the spectral contributions from the white and the red dwarfs, if the pre-CV hypothesis would turn out to be true. Also good spectrophotometric calibration will allow to determine precise spectral types, luminosities, surface temperatures and gravities, thus providing a preliminary astrophysical characterization of these systems. The same spectrophotometric calibration will yield separate and accurate synthetic photometry for both components, if present, in different broad and intermediate band systems.

  5. Acylated anthocyanins from sprouts of Raphanus sativus cv. Sango: isolation, structure elucidation and antioxidant activity.

    PubMed

    Matera, Riccardo; Gabbanini, Simone; Berretti, Serena; Amorati, Riccardo; De Nicola, Gina Rosalinda; Iori, Renato; Valgimigli, Luca

    2015-01-01

    Little is known on structure-activity relationships of antioxidant anthocyanins. Raphanus sativus cv Sango sprouts are among the richest sources (270 mg/100 g fresh weight). We isolated from sprouts' juice 9 acylated anthocyanins, including 4 new compounds. All comprise a cyanidin core bearing 3-4 glucose units, multiply acylated with malonic and phenolic acids (ferulic and sinapic). All compounds were equally effective in inhibiting the autoxidation of linoleic acid in aqueous micelles, with rate constant for trapping peroxyl radicals kinh=(3.8 ± 0.7) × 10(4)M(-1)s(-1) at 37 °C. In acetonitrile solution kinh varied with acylation: (0.9-2.1) × 10(5)M(-1)s(-1) at 30 °C. Each molecule trapped a number n of peroxyl radicals ranging from 4 to 7. Anthocyanins bearing sinapic acid were more effective than those bearing the ferulic moiety. Under identical settings, deacylated cyanin, ferulic and sinapic acids had kinh of 0.4 × 10(5), 0.3 × 10(5) and 1.6 × 10(5)M(-1)s(-1) respectively, with n ranging 2-3. Results show the major role of acylation on antioxidant performance. PMID:25053073

  6. Demonstration of REE fractionation among individual chondrules from the allende (CV3) chondrite

    SciTech Connect

    Misawa, K; Nakamura, N. (Kobe Univ. (Japan))

    1988-06-01

    Abundances of REE, Ba, Sr, Rb, K, Mg and Ca were determined by precise mass spectrometric isotope dilution techniques for 24 chondrules from the Allende (CV3) chondrite. The REE abundances are 2.5-10 {times} CI for barred olivine chondrules, 2-8 {times} CI for porphyritic and nonporphyritic pyroxene chondrules, 0.15-4 {times} CI for porphyritic olivine and porphyritic olivine-pyroxene chondrules and are more or less similarly fractionated. General REE fractionations and large (up to 170%) anomalies of Ce, Eu and Yb occur in all chondrule types, particularly for barred olivine and pyroxene-rich chondrules. Positive correlations of REE with the moderately volatile elements, K and Rb, as well as other refractory elements, Ca, Sr and Ba, are independent of textural type and major chemical compositions. Each type of chondrule has large and systematic abundance variations of K and Rb, but shows a constant K/Rb ratio close to that of CIs. From the these results, the following constraints on the chemical characteristics of precursors and chondrule-forming events are suggested: (1) vaporization loss of alkalis accompanied by K/Rb fractionation did not occur during chondrule-formation melting events, (2) elemental abundances were basically established prior to melting events by accretion of alkali-free component(s) and alkali-bearing refractory precursors with fractionated REE, (3) gas/solid (or liquid) processes yielding REE fractionations took place during the formation of refractory precursors.

  7. Irrigation effects on quality, phenolic composition, and selected volatiles of virgin olive oils cv. Leccino.

    PubMed

    Servili, Maurizio; Esposto, Sonia; Lodolini, Enrico; Selvaggini, Roberto; Taticchi, Agnese; Urbani, Stefania; Montedoro, Gianfrancesco; Serravalle, Matteo; Gucci, Riccardo

    2007-08-01

    Field-grown olive trees (Olea europaea L. cv. Leccino) were used over two growing seasons to determine the effect of deficit irrigation regimes on virgin olive oil (VOO) quality. Drip irrigation was managed to maintain a predawn leaf water potential (PLWP): (a) higher than -1.1 MPa (full irrigation: FI); (b) between -1.0 and -3.3 MPa (deficit irrigation: DI); (c) higher than -4.2 MPa (severe deficit irrigation: SI). The fruit yield and oil yield of DI trees were over 90% of those of FI treatments in both years, respectively, whereas yields of SI trees ranged from 61 to 76%. The irrigation regime had minor effects on the free acidity, peroxide value, and fatty acid composition of VOO. The concentrations of phenols and o-diphenols in VOO were negatively correlated with PLWP. The concentrations of the dialdehydic form of decarboxymethyl elenolic acid linked to (3,4-dihydroxyphenyl)ethanol (3,4-DHPEA-EDA), the isomer of the oleuropein aglycon (3,4-DHPEA-EA), and the dialdehydic form of decarboxymethyl elenolic acid linked to (p-hydroxyphenyl)ethanol (p-HPEA-EDA) were lower in FI than in SI treatments. The concentrations of lignans (+)-1-acetoxipinoresinol and (+)-1-pinoresinol were unaffected by the irrigation regime. The tree water status had a marked effect on the concentration of volatile compounds, such as the C(6)-saturated and unsaturated aldehydes, alcohols, and esters. PMID:17636938

  8. Physiology of Movements in Stems of Seedling Pisum sativum L. cv Alaska 1

    PubMed Central

    Britz, Steven J.; Galston, Arthur W.

    1982-01-01

    The relationship between the apical hook and stem nutation in etiolated Alaska pea (Pisum sativum L. cv Alaska) seedlings was explored. The hook and maximum nutational displacement have the same plane of symmetry, and both are affected by light acting through phytochrome. However, the two processes do not appear to be obligatorily coupled. Light effects on nutation involve at least two components, an increase in amplitude as well as an increase in frequency. These components can be separated from one another on the basis of developmental time course or red light fluence. Excision of the plumule, leaving the hook attached to the stem, inhibits photostimulated nutation. This inhibition can be overcome by application of indole-3-acetic acid to the remaining stem. If the hook is also excised, then nutation in the stem cannot be restored by indole-3-acetic acid. It is possible, although not yet proven, that the oscillatory process regulating nutation in the stem is itself localized in the hook and that rhythms in the transport of indole-3-acetic acid are involved. PMID:16662687

  9. Physiology of Movements in the Stems of Seedling Pisum sativum L. cv Alaska 1

    PubMed Central

    Britz, Steven J.; Galston, Arthur W.

    1983-01-01

    Phototropic response in etiolated pea (Pisum sativum L. cv Alaska) seedlings is poor. However, the curvature induced by unilateral blue light can be hastened and increased in magnitude by a previously administered red light pulse followed by several hours of darkness. Phytochrome is involved in the red light effect. Phototropic response was almost completely inhibited by removal of the apical bud and hook, but it was restored if exogenous indole-3-acetic acid was applied apically to the cut stump. Therefore, the stem contains both the phototropic photoreceptor and response mechanism. Perception of gravity and gravitropic response were also localized in the stem, but gravitropism was scarcely inhibited by decapitation. It was also observed that the kinetics and curvature pattern of gravitropism differed greatly from those of phototropism. Like phototropism, stem nutation required auxin and was promoted by red light. Unlike phototropism, photoenhanced nutational curvature required the apical hook and was propagated as a wave down the stem. Naphthylphthalamic acid inhibited, in order of decreasing effect, nutation, phototropism/gravitropism, and growth. Phototropism, gravitropism, and nutation appear to represent distinct forms of stem movement with fundamental differences in the mechanisms of curvature development. Images Fig. 3 PMID:16662824

  10. Role of Ethylene in Lactuca sativa cv `Grand Rapids' Seed Germination

    PubMed Central

    Abeles, Fred B.

    1986-01-01

    Promotion of thermoinhibited (30°C) lettuce (Lactuca sativa cv `Grand Rapids') seed germination by ethylene is similar to the action of the gas in other hormonal systems. Ethylene was more active than propylene and ethane was inactive. An inhibitor of ethylene production, aminoethoxy-vinylglycine, reduced ethylene evolution and germination. Inhibitors of ethylene action such as, 5-methyl-7-chloro-4-ethoxycarbonylmethoxy-2,1,3-benzothiadiazole, 2,5-norbornadiene, and silver thiosulfate inhibited germination and the effect was reversed by the addition of ethylene to the gas phase. The action of ethylene appears to be due to the promotion of radial cell expansion in the embryonic hypocotyl. The action of N6-benzyladenine and fusiccocin, which also overcome thermoinhibition, appears to be due to a promotion of hypocotyl elongation. None of the germination promoters studied appeared to function by lowering the mechanical resistance of the endosperm to embryonic growth. Data presented here are consistent with the view that ethylene plays a role in lettuce seed germination under thermoinhibited and normal conditions. PMID:16664902

  11. Role of Ethylene in Lactuca sativa cv ;Grand Rapids' Seed Germination.

    PubMed

    Abeles, F B

    1986-07-01

    Promotion of thermoinhibited (30 degrees C) lettuce (Lactuca sativa cv ;Grand Rapids') seed germination by ethylene is similar to the action of the gas in other hormonal systems. Ethylene was more active than propylene and ethane was inactive. An inhibitor of ethylene production, aminoethoxy-vinylglycine, reduced ethylene evolution and germination. Inhibitors of ethylene action such as, 5-methyl-7-chloro-4-ethoxycarbonylmethoxy-2,1,3-benzothiadiazole, 2,5-norbornadiene, and silver thiosulfate inhibited germination and the effect was reversed by the addition of ethylene to the gas phase. The action of ethylene appears to be due to the promotion of radial cell expansion in the embryonic hypocotyl. The action of N6-benzyladenine and fusiccocin, which also overcome thermoinhibition, appears to be due to a promotion of hypocotyl elongation. None of the germination promoters studied appeared to function by lowering the mechanical resistance of the endosperm to embryonic growth. Data presented here are consistent with the view that ethylene plays a role in lettuce seed germination under thermoinhibited and normal conditions. PMID:16664902

  12. Folate levels and polyglutamylation profiles of papaya (Carica papaya cv. Maradol) during fruit development and ripening.

    PubMed

    Ramos-Parra, Perla A; García-Salinas, Carolina; Hernández-Brenes, Carmen; de la Garza, Rocío I Díaz

    2013-04-24

    Folates are essential micronutrients for humans, and their deficiency causes several detrimental effects on human health. Papaya fruit is an important natural source of some micronutrients. This paper presents a first complete characterization of folate derivatives accumulated in cv. Maradol papaya during fruit development and ripening processes. During postharvest ripening, the fruit accumulated up to 24.5% of the daily folate recommended dietary allowance (RDA) for an adult in a 1 cup (145 g) portion. Tetrahydrofolate (THF) and 5-methyl-THF were the predominant folate classes observed. Surprisingly, an unusually long polyglutamylation profile of tentatively up to 17 glutamates linked to 5-methyl-THF was detected; to the authors' knowledge, this very long polyglutamyl tail has not been reported for any organism, and it is probably characteristic of this plant species. This polyglutamylation degree changed throughout fruit development and ripening, showing the largest differences at the onset of ripening. This work raises questions about the functional role of folate derivatives in fruit development. PMID:23574547

  13. Priming and temperature limits for germination of dispersal units of Urochloa brizantha (Stapf) Webster cv. basilisk.

    PubMed

    Nakao, Ea; Cardoso, Vjm

    2015-01-01

    The objective of this study was to evaluate the effect of priming treatments on the upper and lower thermal limits for germination of Urochloa brizantha cv. basilisk, and testing the hypothesis that pré-imbibition affect thermal parameters of the germination. Pre-imbibed seeds both in distilled water (0 MPa) and PEG 6000 solution (-0.5 MPa) were put to germinate in different temperatures. It is suggested that U. brizantha seeds have low response to priming when they were placed to germinate in medium where water is not limiting. The response of U. brizantha seeds to priming is dependent on the temperature and water potential conditions at which the seeds are pre-imbibed, as well as on the germination temperature. The optimum temperature for germination of U. brizantha shift toward warmer temperatures in primed seeds. Priming effect was more pronounced at temperatures closer to the upper and lower limit for germination, but probably that response cannot be accounted for changes in the thermal time constant (?T(g)) and ceiling temperature (Tc(g)). Otherwise, a decrease in the base temperature (Tb) was observed in primed seeds, suggesting that the Tb distribution in U. brizantha seeds is influenced by priming. PMID:25945643

  14. Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)

    PubMed Central

    Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng

    2009-01-01

    A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 ?M, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 ?M. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172

  15. The BANANA Project. V. Misaligned and Precessing Stellar Rotation Axes in CV Velorum

    NASA Astrophysics Data System (ADS)

    Albrecht, Simon; Winn, Joshua N.; Torres, Guillermo; Fabrycky, Daniel C.; Setiawan, Johny; Gillon, Michaël; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

    2014-04-01

    As part of the Binaries Are Not Always Neatly Aligned project (BANANA), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of ?p = -52° ± 6° and ?s = 3° ± 7° for the primary and secondary stars (B2.5V + B2.5V, P = 6.9 days). We combine this information with several measurements of changing projected stellar rotation speeds (vsin i sstarf) over the last 30 yr, leading to a model in which the primary star's obliquity is ?65°, and its spin axis precesses around the total angular momentum vector with a period of about 140 yr. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the vsin i sstarf. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronger misalignment similar to DI Herculis, a younger but otherwise comparable binary. Based on observations made with ESOs 2.2 m Telescopes at the La Silla Paranal Observatory under programme ID 084.C-1008 and under MPIA guaranteed time.

  16. In vitro antioxidant activity of banana (Musa spp. ABB cv. Pisang Awak).

    PubMed

    Darsini, D Teepica Priya; Maheshu, V; Vishnupriya, M; Sasikumar, J M

    2012-04-01

    The methanolic extract of Musa ABB cv Pisang Awak was investigated for the polyphenolic contents and antioxidant activity. The total phenol and flavonoid contents of the fruit extract were found to be 120 mg gallic acid equivalents (GAE) and 440 mg quercetin equivalents (QE)/100 g of sample dry weight, respectively. The antioxidant activity of the Pisang Awak methanol extract (PAME) (20-500 microg/ml) was determined using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing capacity, 2-2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) radical cation decolourization and hydroxyl radical scavenging capacity (OH*). The EC50 values of DPPH, ABTS and OH* activities of the PAME and butylated hydroxy toluene (BHT) were found to be 65 and 9 microg/ml, 29 and 6 microg/ml, 36 and 42 microg/ml respectively. The reducing capacity increased with increasing concentration (31.5-1000 mg/ml) of the fruit extract and the activity was comparable with the standard BHT. The high performance thin layer chromatography (HPTLC) analysis of the extract revealed the presence of polyphenols. The strong and positive correlations were obtained between total phenol/flavonoid contents (R2 = 0.693-1.0) and free radical scavenging ability was attributed to the polyphenols as the major antioxidants. PMID:22650010

  17. [In vitro and in vivo effects of mango pulp (Mangifera indica cv. Azucar) in colon carcinogenesis].

    PubMed

    Corrales-Bernal, Andrea; Amparo Urango, Luz; Rojano, Benjamín; Maldonado, Maria Elena

    2014-03-01

    Mango pulp contains ascorbic acid, carotenoids, polyphenols, terpenoids and fiber which are healthy and could protect against colon cancer. The aim of this study was to evaluate the antiproliferative and preventive capacity of an aqueous extract of Mangifera indica cv. Azúcar on a human colon adenocarcinoma cell line (SW480) and in a rodent model of colorectal cancer, respectively. The content of total phenolics, flavonoids and carotenoids were also analyzed in the extract. SW480 cell growth was inhibited in a dose and time dependent manner by 22.3% after a 72h exposure to the extract (200 µg/ mL). Colon carcinogenesis was initiated in Balb/c mice by two intra-peritoneal injections of azoxymethane (AOM) at the third and fourth week of giving mango in drinking water (0.3%, 0.6%, 1.25%). After 10 weeks of treatment, in the colon of mice receiving 0.3% mango, aberrant crypt foci formation was inhibited more than 60% (p=0,05) and the inhibition was dose-dependent when compared with controls receiving water. These results show that mango pulp, a natural food, non toxic, part of human being diet, contains bioactive compounds able to reduce growth of tumor cells and to prevent the appearance of precancerous lesions in colon during carcinogenesis initiation. PMID:25796713

  18. Diurnal cycles of embolism formation and repair in petioles of grapevine (Vitis vinifera cv. Chasselas)

    PubMed Central

    Zufferey, V.; Cochard, H.; Ameglio, T.; Spring, J.-L.; Viret, O.

    2011-01-01

    The impact of water deficit on stomatal conductance (gs), petiole hydraulic conductance (Kpetiole), and vulnerability to cavitation (PLC, percentage loss of hydraulic conductivity) in leaf petioles has been observed on field-grown vines (Vitis vinifera L. cv. Chasselas). Petioles were highly vulnerable to cavitation, with a 50% loss of hydraulic conductivity at a stem xylem water potential (?x) of –0.95?MPa, and up to 90% loss of conductivity at a ?x of –1.5?MPa. Kpetiole described a daily cycle, decreasing during the day as water stress and evapotranspiration increased, then rising again in the early evening up to the previous morning's Kpetiole levels. In water-stressed vines, PLC increased sharply during the daytime and reached maximum values (70–90%) in the middle of the afternoon. Embolism repair occurred in petioles from the end of the day through the night. Indeed, PLC decreased in darkness in water-stressed vines. PLC variation in irrigated plants showed the same tendency, but with a smaller amplitude. The Chasselas cultivar appears to develop hydraulic segmentation, in which petiole cavitation plays an important role as a ‘hydraulic fuse’, thereby limiting leaf transpiration and the propagation of embolism and preserving the integrity of other organs (shoots and roots) during water stress. In the present study, progressive stomatal closure responded to a decrease in Kpetiole and an increase in cavitation events. Almost total closure of stomata (90%) was measured when PLC in petioles reached >90%. PMID:21447755

  19. Visualisation and quantification of CV chondrite petrography using micro-tomography

    NASA Astrophysics Data System (ADS)

    Hezel, Dominik C.; Elangovan, Premkumar; Viehmann, Sebastian; Howard, Lauren; Abel, Richard L.; Armstrong, Robin

    2013-09-01

    Micro-computed tomography is a non-destructive technique that allows the study of 3D meteorite petrography. The technique produces a unique and instructive visualisation of the meteorite for quantifying its components. We studied the overall petrography of the two CV chondrites Allende and Mokoia to constrain their formation histories. A set of movies and stereographic images detail the 3D petrography. Component modal abundances agree with previous reports and modal abundance differences between Allende and Mokoia support the chondrule-matrix complementarity and that chondrules and matrix formed from the same chemical reservoir. We identified two types of chondrules, a normal type and one where a normal type I or II chondrule is almost completely encapsulated by an opaque-rich layer. This layer was probably acquired during a late stage condensation process. The appearance of opaques in chondrules and matrix is different, not supporting a genetic relationships between these. Low abundances of compound chondrules (1.75 vol% in Allende and 2.50 vol% in Mokoia) indicate low chondrule densities and/or low relative component velocities in chondrule formation regions. Porosities on a scale <10-20 ?m allowed for only local aqueous alteration processes on the meteorite parent bodies.

  20. Induction of Flavonoid Synthesizing Enzymes by Light in Etiolated Pea (Pisum sativum cv. Midfreezer) Seedlings

    PubMed Central

    Hrazdina, Geza; Parsons, Grace F.

    1982-01-01

    Etiolated pea (Pisum sativum cv. Midfreezer) seedlings respond to illumination with white light by changes in the activity of phenylpropanoid and flavonoid synthesizing enzymes. Unlike in cell cultures, changes in enzyme activity in pea seedlings are not concerted. Phenylalanine ammonia-lyase (EC 4.3.1.5) activity peaked approximately 18 hours after onset of illumination. The phenylacetate path did not interfere with the measurement of phenylalanine ammonia-lyase activity. Activity of cinnamic acid 4-hydroxylase (EC 1.14.13.11) showed an early peak after 8 hours illumination, declined thereafter sharply, then gradually increased during the remainder of the experiment. Activities of chalcone synthase and UDP glucose:flavonol 3-O-glucosyltransferase (EC 2.4.1.91) increased steadily and reached a plateau after approximately 70 hours illumination time. Activity of 4-hydroxycinnamate:coenzyme A ligase (EC 6.2.1.12) remained relatively unchanged, whereas that of chalcone isomerase (EC 5.5.1.6) declined steadily during the course of the experiment. The relative in vitro enzyme activities suggest that the rate-limiting step for the phenylpropanoid path is the cinnamic acid 4-hydroxylase, that of the flavonoid pathway is the chalcone synthase. Integration of enzyme activity curves, however, show that only the curve deriving from phenylanine ammonia-lyase activity matches closely the production of the flavonol glycosides. PMID:16662524

  1. The BANANA project. V. Misaligned and precessing stellar rotation axes in CV Velorum

    E-print Network

    Albrecht, Simon; Torres, Guillermo; Fabrycky, Daniel C; Setiawan, Johny; Gillon, Michael; Jehin, Emmanuel; Triaud, Amaury; Queloz, Didier; Snellen, Ignas; Eggleton, Peter

    2014-01-01

    As part of the BANANA project (Binaries Are Not Always Neatly Aligned), we have found that the eclipsing binary CV Velorum has misaligned rotation axes. Based on our analysis of the Rossiter-McLaughlin effect, we find sky-projected spin-orbit angles of $\\beta_{\\rm p} = -52\\pm6^{\\circ}$ and $\\beta_{\\rm s}= 3\\pm7^{\\circ}$ for the primary and secondary stars (B2.5V $+$ B2.5V, $P=6.9$ d). We combine this information with several measurements of changing projected stellar rotation speeds ($v \\sin i_{\\star}$) over the last $30$ years, leading to a model in which the primary star's obliquity is $\\approx65^{\\circ}$, and its spin axis precesses around the total angular momentum vector with a period of about $140$ years. The geometry of the secondary star is less clear, although a significant obliquity is also implicated by the observed time variations in the $v \\sin i_{\\star}$. By integrating the secular tidal evolution equations backward in time, we find that the system could have evolved from a state of even stronge...

  2. Using MOST to reveal the secrets of the mischievous Wolf-Rayet binary CV Ser

    NASA Astrophysics Data System (ADS)

    David-Uraz, Alexandre; Moffat, Anthony F. J.; Chené, André-Nicolas; Rowe, Jason F.; Lange, Nicholas; Guenther, David B.; Kuschnig, Rainer; Matthews, Jaymie M.; Rucinski, Slavek M.; Sasselov, Dimitar; Weiss, Werner W.

    2012-11-01

    The Wolf-Rayet (WR) binary CV Serpentis (= WR113, WC8d + O8-9IV) has been a source of mystery since it was shown that its atmospheric eclipses change with time over decades, in addition to its sporadic dust production. The first high-precision time-dependent photometric observations obtained with the Microvariability and Oscillations of STars (MOST) space telescope in 2009 show two consecutive eclipses over the 29-d orbit, with varying depths. A subsequent MOST run in 2010 showed a seemingly asymmetric eclipse profile. In order to help make sense of these observations, parallel optical spectroscopy was obtained from the Mont Megantic Observatory (2009, 2010) and from the Dominion Astrophysical Observatory (2009). Assuming these depth variations are entirely due to electron scattering in a ?-law wind, an unprecedented 62 per cent increase in M? is observed over one orbital period. Alternatively, no change in mass-loss rate would be required if a relatively small fraction of the carbon ions in the wind globally recombined and coaggulated to form carbon dust grains. However, it remains a mystery as to how this could occur. There also seems to be evidence for the presence of corotating interaction regions (CIR) in the WR wind: a CIR-like signature is found in the light curves, implying a potential rotation period for the WR star of 1.6 d. Finally, a new circular orbit is derived, along with constraints for the wind collision.

  3. Effect of chemical pretreatment on dehulling parameters of flaxseed (cv. Garima).

    PubMed

    Mridula, D; Barnwal, P; Gurumayum, Sushma; Singh, K K

    2014-09-01

    In the present study, effects of chemical (ethanol, HCl and sulphuric acid) pretreatment on various dehulling parameters of flaxseed (cv. Garima) including yield, hull, hullability, extraction rate and embryo (dehulled flaxseed) recovery were studied. Pretreated flaxseed, at 3.1 to 3.6 % moisture range (p?>?0.05) were dehulled for 60 s in a laboratory model rice polisher/dehulling machine at 2,000 rpm followed by aspiration (hull separation) using a laboratory model aspirator. The study revealed that chemical pre-dehulling treatment of flaxseed plays a significant role in the embryo recovery of flaxseed. Both ethanol and HCl pre-dehulling treatment enhanced but sulphuric acid pretreatment reduced the embryo recovery of flaxseed. Moreover, HCL and Sulphuric acid deteriorated the quality of hull obtained during dehulling, hence may not be considered for flaxseed dehulling. The study showed the maximum embryo recovery from ethanol pretreated flaxseed, hence ethanol pre-dehulling treatment with 2 h tempering time may be considered for effective flaxseed dehulling. PMID:25190888

  4. Case study of chondrule alteration with IR spectroscopy in NWA 2086 CV3 meteorite

    NASA Astrophysics Data System (ADS)

    Kereszturi, A.; Gyollai, I.; Szabó, M.

    2015-02-01

    Analyzing the alteration in an olivine chondrule of the NWA 2086 CV3 meteorite, infrared spectral, electron microprobe and optical microscopic observations were correlated to each other. The intensity and wavelength positions of olivine peaks changed characteristically with the progression of alteration and related Fe/Mg substitution inward of the chondrule. Moderate to good correlations were identified between Fo% composition and positions of 830 and 860 cm-1 IR peaks. The disappearance of 1020 cm-1 peak by structural change happens already at a low level alteration without changing the optical appearance of the mineral. The existence of the 980 cm-1 peak is found to be an indicator of the intact phase of olivine. While profiles perpendicular to the chondrule's perimeter showed that the alteration progressed 15-20 ?m distance inward without observable fractures (probablly by some diffusion related process), the "alteration distance" from various obvious fractures inside the chondrule was only 3-5 ?m distance. These observations suggest that the substitution was more effective close to the matrix, and also related to some fluids that although were able to circulate along the large internal fractures too, did not produce such strong substitution there, like what happened close to the matrix. It was also demonstrated that the poorly exploited contact mode observations with ATR based reflection method in infrared spectroscopy provide a useful tool to analyze the alteration at micrometer scale without much sample preparation, and enable identifying alterations already at such a low level where the olivines still look optically intact.

  5. Nutraceutical properties and polyphenolic profile of berry skin and wine of Vitis vinifera L. (cv. Aglianico).

    PubMed

    De Nisco, Mauro; Manfra, Michele; Bolognese, Adele; Sofo, Adriano; Scopa, Antonio; Tenore, Gian Carlo; Pagano, Francesco; Milite, Ciro; Russo, Maria Teresa

    2013-10-15

    Red grapes are rich in phenolics, flavonoids, anthocyanins and resveratrol, all substances which have been suggested as having nutraceutical and health benefits. The berry skin and wine of grape cultivar Vitis vinifera L. (cv. Aglianico), grown in Basilicata (Southern Italy) were examined to determinate the presence of the above mentioned compounds as well as to establish the inorganic cation profile. HPLC analysis coupled with LC-ESI/MS/MS detected high contents of total flavonols and anthocyanins in berry skin and wine. The wine made with the same grape used for berry skin assays showed a notable presence of quercetin-3-O-glucoside (39.4% of total flavonols), and malvidin and petunidin derivatives (63.9% and 10.8% of total anthocyanins, respectively). The strong antioxidant ROS-scavenging activity, determined by both DPPH and FRAP assays, and the high resveratrol content confer high sensory characteristics resulted to be associated with positive nutraceutical properties of these grapes and wine. The level of cis-resveratrol was lower than trans-resveratrol in both berry skin and wine reaching 44.1mg/kg and 0.3mg/l, respectively. The cation profile presents low levels of Ca, Cu, K, Fe, Zn and Cd compared to numerous, important red wines, such as Monastrell and Tempranillo. PMID:23692745

  6. Biomonitoring of air pollution with mercury in Croatia by using moss species and CV-AAS.

    PubMed

    Spiri?, Zdravko; Vu?kovi?, Ivana; Stafilov, Traj?e; Kušan, Vladimir; Ba?eva, Katerina

    2014-07-01

    Moss samples from four dominant species (Hypnum cupressiforme, Pleurozium schreberi, Homalothecium sericeum and Brachythecium rutabulum) were collected during the summer and autumn of 2010 from 121 sampling sites evenly distributed over the territory of Croatia. Samples were totally digested by using microwave digestion system, whilst mercury was analysed by using cold vapour atomic absorption spectrometry (CV-AAS). Descriptive statistics were done from analyses of mercury in all moss samples. The content of mercury ranged from 0.010 to 0.145 mg kg(-1) with a median value of 0.043 mg kg(-1). Hg distribution map shows the sites of the country with higher levels of this element. High contents of Hg were found in moss samples collected from the regions of Podravina and Istria as a result of anthropogenic pollution. Comparison of median values and ranges with those found in moss samples in 2006 shows slight reduction of mercury air pollution. When compared to the results obtained from recent studies conducted in Slovenia, Macedonia and especially in Norway-which serves as a reference considering the fact that it is a pristine area-mercury air pollution in Croatia is insignificant. PMID:24733436

  7. Effect of sowing dates and vernalization on Beta vulgaris L. cv. Univers C-leaf structure.

    PubMed

    Sakr, Mohammed M; Almaghrabi, Omar A

    2011-07-01

    This research was conducted to study the effect of three different sowing dates (15th October, 15th November and 15th December) and two vernalization treatments (5 °C and -20 °C) on leaf structure of Beta vulgaris L. cv. Univers. The obtained data are summarized as follows: The maximum values of the most studied parameters; lower epidermis + spongy tissue thickness, midrib, mesophyll tissue, vascular bundle, collenchymatous tissue and number of xylem vessels per arm were found as a result of 15th October sowing date treatment compared with the two other sowing dates. Furthermore, effect of the cooling treatments varied according to the recorded character, sowing date and cooling degree. Most of the vernalization treatments at early sowing dates increased the mesophyll tissue, midrib, number of vascular bundles per transverse section, vascular bundle thickness and number of xylem arms per transverse section. The two studied cooling treatments at 15th October sowing date increased both stomatal index and average number of stomata: average number of epidermis cells compared with the control. Furthermore, 15th October under -20 °C treatment led to small epidermal cells and stomata formation, straight epidermal cell walls and closed stomata in comparison to the control. PMID:23961134

  8. The mineral chemistry and origin of inclusion matrix and meteorite matrix in the Allende CV3 chondrite

    NASA Technical Reports Server (NTRS)

    Kornacki, A. S.; Wood, J. A.

    1984-01-01

    The mineralogy and mineral chemistry of the inclusion and meteorite matrices in the Allende CV3 chondrite are described, and the physical and chemical parameters of the conventional equilibrium condensation model of the origin of chondrite meteorites are evaluated. An alternative model of the origin of the mafic constituent of Allende inclusions is presented, on the basis of a new model of chondrule petrogenesis and the physical evolution of the primitive solar nebula. The model shows that the mineral chemistry of the olivine matrix in Allende CV3 seems to preserve a good record of nebular and planetary processes, including: (1) vapor-to-solid condensation under relatively oxidizing nonequilibrium conditions; (2) Fe/Mg equilibration in the meteorite parent body; and (3) recrystallization and incipient melting in the solar nebula.

  9. Xanthine Oxidase Inhibitor Activity of Terpenoid and Pyrrole Compounds Isolated from Snake Fruit (Salacca edulis Reinw.) cv. Bongkok

    NASA Astrophysics Data System (ADS)

    Herliani Afrianti Priyatno, Leni; Yulinah Sukandar, Elin; Ibrahim, Slamet; Ketut Adnyana, I.

    The compound of 3β-hydroxy-sitosterol (1) and 2-metylester-1-H-pyrrole-4-carboxilyc acid were isolated from ethyl acetate extract of snake fruit (Salacca edulis Reinw) cv. Bongkok, (2). Inhibition of xanthine oxidase by the two compounds were evaluated against enzyme of xanthine oxidase. Compound 1 could be regarded as inactive, while compound 2 was found to be active with IC50 value of 48.86 μg mL-1.

  10. Plant Regeneration from Immature Inflorescence Culture and Genetic Transformation of Wheatgrass ( Agropyron cristatum × A. desertorum cv. Hycrest-Mengnong)

    Microsoft Academic Search

    Xiu-wen HUO; Jian-hua WEI; Chun-bo XU; Fu-gui MI; Jin-feng YUN

    2006-01-01

    The plants of hybrid wheatgrass (A. cristatum × A. desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on improved MS medium containing 2.0-3.0 mg L?1 2,4-D to regenerate callus. The calli were then transferred to hormone-free MS medium for differentiation and 1\\/2 MS medium for rooting. Results showed that callus initiation

  11. Interpreting the nonideal reverse bias CV characteristics and importance of the dependence of Schottky barrier height on applied voltage

    Microsoft Academic Search

    Abdulmecit Turut; Mustafa Saglam; Hasan Efeoglu; Necati Yalcin; Muhammed Yildirim; Bahattin Abay

    1995-01-01

    This work presents an attempt related to the charging behaviour of interface states to the nonideal forward bias current-voltage (I-V) and the reverse bias capacitance-voltage (C-V) characteristics of Al?nSi Schottky barrier diodes. The diode showed nonideal I-V behaviour with an ideality factor of 1.50 and was thought to have a metal-interface layer-semiconductor configuration. Considering that the interface states localized at

  12. Effect of plant growth regulators on seed yield in velvet grass (Holcus lanatus L) cv. Massey Basyn

    Microsoft Academic Search

    J. G. Hampton; M. S. Tolentino; M. J. Hill

    1992-01-01

    Paclobutrazol applied at spikelet initiation at rates of 1.0 and 2.0 kg a.i.\\/ha increased seed yield of a first year crop of velvet grass cv. Massey Basyn from 55.9 g\\/m to 83.5 and 74.4 g\\/m respectively. Although stem length was reduced and lodging prevented, the seed yield response came from increased fertile tiller production, as other yield components did not

  13. Origin of magnetite in oxidized CV chondrites: in situ measurement of oxygen isotope compositions of Allende magnetite and olivine

    Microsoft Academic Search

    Byeon-Gak Choi; Kevin D. McKeegan; Laurie A. Leshin; John T. Wasson

    1997-01-01

    Magnetite in the oxidized CV chondrite Allende mainly occurs as spherical nodules in porphyritic-olivine (PO) chondrules, where it is associated with Ni-rich metal and\\/or sulfides. To help constrain the origin of the magnetite, we measured oxygen isotopic compositions of magnetite and coexisting olivine grains in PO chondrules of Allende by an in situ ion microprobe technique. Five magnetite nodules form

  14. Photosynthetic responses of pea plants (Pisum sativum L. cv. Little marvel) exposed to climate change in Riyadh city, KSA

    Microsoft Academic Search

    Akram Ali; Ahmed Alfarhan; Ibrahim Aldjain; Nagat Bokhari; Khaled Al-Rasheid; Saleh Al-Quraishi

    Pots study was conducted to determine interactive effects of climate change (NO2, SO2 and O3) on photosynthetic responses in pea. The seeds of pea plants (Pisum sativum L. cv. Little marvel) were grown full-season in three pots arranged at four localities in Riyadh city, KSA. Photosynthetic rates (Pn) were measured three times during vegetative and reproductive stages with portable gas

  15. The Indirect Keyboard Control System by Using the Gaze Tracing Based on Haar Classifier in OpenCV

    Microsoft Academic Search

    Chang-Zheng Li; Chung-Kyue Kim; Jong-Seung Park

    2009-01-01

    This paper describes on the indirect interface system in which general users assign computer instructions just through the gaze tracing, without mouse or keyboard. We use the Web camera to replace the computer-input system. The face region and the eye region was extracted based on Haar classifier implemented on open source computer vision library (OpenCV). It controls mouse-moving by automatically

  16. OpenCV C# wrapper based video enhancement using different optical flow methods in the super-resolution

    Microsoft Academic Search

    A. Nagy; Z. Vamossy

    2008-01-01

    This article presents a simple method on how to implement a super-resolution based video enhancement technique in .NET using the functions of the OpenCV library. First, we outline the goal of this project and after that, a short review of the steps of super-resolution technique is given. As a part of the discussion about the concrete implementation, firstly the general

  17. A new technique to determine the average low-field electron mobility in MESFET using CV measurement

    Microsoft Academic Search

    Byung-jong Moon; Max J. Helix; Seongheam Lee

    1992-01-01

    A method to determine the average low-field mobility using the number of electrons available for the conduction based on C-V measurement is proposed. This technique requires neither information of the doping profile in the channel, nor the exact value of the threshold voltage. For a D-mode MESFET, the average electron mobility magnitude is compared with that of the C. Chen

  18. Characterization of bisphenol A metabolites produced by Portulaca oleracea cv. by liquid chromatography coupled with tandem mass spectrometry.

    PubMed

    Watanabe, Ippei; Harada, Kazuo; Matsui, Takeshi; Miyasaka, Hitoshi; Okuhata, Hiroshi; Tanaka, Satoshi; Nakayama, Hideki; Kato, Ko; Bamba, Takeshi; Hirata, Kazumasa

    2012-01-01

    The garden plant portulaca (Portulaca oleracea cv.) efficiently removes bisphenol A (BPA), an endocrine-disrupting chemical, from a hydroponic solution, but the molecular mechanisms underlying BPA metabolism by portulaca remain unclear. In this study, BPA metabolites converted by portulaca were analyzed by liquid chromatography coupled with tandem mass spectrometry. We observed the hydroxylation of BPA and the oxidization of it to quinone. Polyphenol oxidases are likely to contribute to BPA degradation by portulaca. PMID:22738977

  19. Chromium uptake and its effects on certain plant nutrients in maize (Zea mays L. cv. Ganga 5)

    Microsoft Academic Search

    D. C. Sharma; R. C. Pant

    1994-01-01

    The effects of chromium at 0.05,0.1,0.25,0.5 and 1.0 mM on iron, manganese, zinc and copper in maize (Zea mays L. cv. Ganga 5) were studied in controlled glass house conditions at ambient temperature after 56 days growth when plants started showing vein?clearing and papery appearence in the leaves. No clear pattern of iron concentration and in general, the reductions in

  20. Biotransformation of C 13 -norisoprenoids and monoterpenes by a cell suspension culture of cv. Gamay ( Vitis vinifera )

    Microsoft Academic Search

    Sandrine Mathieu; Jérémie Wirth; François-Xavier Sauvage; Jean-Paul Lepoutre; Raymond Baumes; Ziya Gunata

    2009-01-01

    A cell suspension culture of cv. Gamay was studied for its ability to metabolize two different C13-norisoprenoidic volatiles, ?-ionone and dehydrovomifoliol, together with monoterpenes, geraniol and linalool, biogenetically\\u000a common pathways sharing compounds. ?-Ionone was totally metabolized leading to fourteen norisoprenoidic volatiles oxygenated\\u000a mainly at carbons 3 or 4 of the cyclohexane ring or reduced at side chain. The biotransformation of

  1. An efficient protoplast-to-plant system for the hybrid ornamental shrub, Weigela × florida cv. Bristol Ruby ( Caprifoliaceae )

    Microsoft Academic Search

    Sergio J. Ochatt

    1993-01-01

    Strategies were developed for the successful isolation of large numbers of highly viable protoplasts from the leaves, stems\\u000a and roots of axenic plants of the hybrid ornamental shrubWeigela florida cv Bristol Ruby. Protoplasts, of all sources, were cultured on different media, leading to the establishment of sustained\\u000a divisions, and coupled with the production of multi-celled (>50 cells) colonies. However, those

  2. Altitudinal Variation of Phenolic Contents in Flowering Heads of Arnica montana cv. ARBO: a 3Year Comparison

    Microsoft Academic Search

    Renate Spitaler; Andrea Winkler; Isabella Lins; Sema Yanar; Hermann Stuppner; Christian Zidorn

    2008-01-01

    In continuation of our studies of altitudinal effects on secondary metabolite profile of flowering heads from taxa of the\\u000a Asteraceae, we investigated phenolic contents and radical scavenging potential from cultivated plants of Arnica montana cv. ARBO during the growing seasons 2003, 2004, and 2005. By conducting experiments on potted plants, we excluded that differences\\u000a in phenolic contents from plants grown

  3. Structural analysis of wheat wax ( Triticum aestivum, c.v . ‘Naturastar’ L.): from the molecular level to three dimensional crystals

    Microsoft Academic Search

    K. Koch; W. Barthlott; S. Koch; A. Hommes; K. Wandelt; W. Mamdouh; S. De-Feyter; P. Broekmann

    2006-01-01

    In order to elucidate the self assembly process of plant epicuticular waxes, and the molecular arrangement within the crystals,\\u000a re-crystallisation of wax platelets was studied on biological and non-biological surfaces. Wax platelets were extracted from\\u000a the leaf blades of wheat (Triticum aestivum L., c.v. ‘Naturastar’, Poaceae). Waxes were analysed by gas chromatography (GC) and mass spectrometry (MS). Octacosan-1-ol was found

  4. Effects of Colonization of the Roots of Domestic Rice (Oryza sativa L. cv. Amaroo) by Burkholderia pseudomallei.

    PubMed

    Prasertsincharoen, Noppadol; Constantinoiu, Constantin; Gardiner, Christopher; Warner, Jeffrey; Elliman, Jennifer

    2015-07-01

    Burkholderia pseudomallei is a saprophytic bacterium that causes melioidosis and is often isolated from rice fields in Southeast Asia, where the infection incidence is high among rice field workers. The aim of this study was to investigate the relationship between this bacterium and rice through growth experiments where the effect of colonization of domestic rice (Oryza sativa L. cv Amaroo) roots by B. pseudomallei could be observed. When B. pseudomallei was exposed to surface-sterilized seeds, the growth of both the root and the aerosphere was retarded compared to that in controls. The organism was found to localize in the root hairs and endodermis of the plant. A biofilm formed around the root and root structures that were colonized. Growth experiments with a wild rice species (Oryza meridionalis) produced similar retardation of growth, while another domestic cultivar (O. sativa L. cv Koshihikari) did not show retarded growth. Here we report B. pseudomallei infection and inhibition of O. sativa L. cv Amaroo, which might provide insights into plant interactions with this important human pathogen. PMID:25911477

  5. A Phase I Trial of CV706, a Replication-competent, PSA Selective Oncolytic Adenovirus, for the Treatment of Locally Recurrent Prostate Cancer following Radiation Therapy1

    Microsoft Academic Search

    Theodore L. DeWeese; Henk van der Poel; Shidong Li; Bahar Mikhak; Marti Goemann; Ulrike Hamper; Robert DeJong; Nicholas Detorie; Ronald Rodriguez; Thomas Haulk; Angelo M. DeMarzo; Steven Piantadosi; D. C. Yu; Yu Chen; Daniel R. Henderson; Michael A. Carducci; William G. Nelson; Jonathan W. Simons

    2001-01-01

    CV706 is a prostate-specific antigen (PSA)-selective, replication- competent adenovirus that has been shown to selectively kill human prostate cancer xenografts in preclinical models. To study the safety and activity of intraprostatic delivery of CV706, a Phase I dose-ranging study for the treatment of patients with locally recurrent prostate cancer after radiation therapy was conducted. Twenty patients in five groups were

  6. CV Characteristics of MOS Structures Fabricated of Al-Doped p-Type 3C-SiC Epilayers Grown on Si by Chemical Vapor Deposition

    Microsoft Academic Search

    Mikiya Shinohara; Mitsugu Yamanaka; Shunji Misawa; Hajime Okumura; Sadafumi Yoshida

    1991-01-01

    MOS structures were fabricated from Al-doped p-type 3C-SiC epilayers grown on Si by chemical vapor deposition. Their C-V characteristics were studied for the first time at 400 Hz, 10 kHz and 100 kHz. Accumulation, depletion, deep depletion and inversion regions were observed in the C-V curves. The increase of capacitance in the inversion region was observed for reverse sweep of

  7. Physiology of Movements in Stems of Seedling Pisum sativum L. cv. Alaska 12

    PubMed Central

    Britz, Steven J.; Galston, Arthur W.

    1982-01-01

    Gravitropism and nutation in the stems of dark-grown, seedling peas (Pisum sativum L. cv. Alaska) were recorded on time-lapse photographs made with photomorphogenetically inactive light. Although gravitropism and nutation have been connected by several different theories in the past, our experiments indicate that the two processes are in fact dissociable. The evidence is as follows: (a) Nutational patterns are asymmetric. There is much greater amplitude of oscillation in the plane parallel (?) to the plane of the apical hook than in the plane perpendicular (?), yet the average gravitropic response is equal in these two planes. (b) Brief red light irradiation given 16 to 24 hours before observation greatly increases the amplitude of nutation in the ?-plane, but has no influence on the kinetics of gravitropic response. (c) An inhibitor of auxin transport, ?-naphthylphthalamic acid, strongly inhibits nutation at 5 micromolar but affects gravitropism only at higher concentrations. (d) Nutation is also strongly inhibited by removal of the apical bud, but gravitropism is unaffected. (e) The period of nutation does not exhibit a constant relationship to the response time of gravitropism. The above evidence is inconsistent with theories that gravitropism is an asymmetrically modified nutation or, alternatively, that nutational oscillations result in a simple fashion from gravitropic overshoots. The evidence is consistent with, although not proof of, autonomous factors such as an endogenous rhythm of growth as the cause of nutation in pea stems. However, gravity and nutation do interact. Nutation in a population of seedlings can be synchronized and brought into phase by a single gravitropic induction. Furthermore, the response time and initial rate of gravitropic curvature depend to some extent on the phase of nutational curvature at which gravitropic induction is begun. PMID:16662458

  8. Nature and origin of interstellar diamond from the Allende CV3 meteorite

    NASA Technical Reports Server (NTRS)

    Blake, David; Freund, Friedemann; Bunch, Ted; Krishnan, Kannan; Stampfer, Mitch; Chang, Sherwood; Tielens, Alexander G. G. M.

    1990-01-01

    Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende CV3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on 3 mm EM grids. The AEM was performed on transmission-thin fragments of the material which overlay holes in the film, to eliminate interference from the substrate. Electron Spectroscopy for Chemical Analysis (ESCA) was performed on a large aliquot of C. Diamond was identified by selected area electron diffraction. Scanning Transmission Electron Microscope / Energy Dispersive X-ray (STEM-EDS) microanalyses of the C delta diamond, using a light-element detector, show that oxygen and possibly nitrogen are the only impurities consistently present. ESCA spectra from bulk C delta material confirm the presence of N at a level of 0.35 percent or less. Under UV irradiation a yellow-red fluorescence is observed, consistent with that of natural diamonds containing substitutional N. Electron Energy Loss Spectra (EELS) were recorded at 2 eV resolution from the C delta diamond, high pressure synthetic diamond, a diamond film produced in a low pressure plasma by chemical vapor deposition (CVD) on a heated silicon substrate (Roy, 1987), graphite, and amorphous arc sputtered carbon. Comparison of the carbon K edge shape and fine structure shows the Allende C delta phase to be largely diamond, but with a significant pre-edge absorption feature indicative of transitions of C 1s electrons into pi asterisk orbitals which are absent in the purely sp(3)-bonded diamond but present in graphite and amorphous carbon.

  9. The toxic effects of mature flatpea (Lathyrus sylvestris L cv Lathco) on sheep.

    PubMed

    Rowe, L D; Ivie, G W; DeLoach, J R; Foster, J G

    1993-04-01

    The toxic effects of mature, seed-bearing flatpea (Lathyrus sylvestris L cv Lathco) hay on sheep was studied in a feeding trial with 25 adult ewes. Five ewes were barren; 20 were in the last 10 w of pregnancy. The ewes were blocked by weight and reproductive status for assignment to treatment groups. Four groups of 5 ewes each were allotted to 1 of the following diets: 0% (controls), 35.0%, 52.5% or 70.0% flatpea (FP); and 1 group of 5 ewes was allotted to a diet of increasing levels of 17.5, 35.0, 52.5 and 70.0% FP. Flatpea hay was harvested in the mature, seed-bearing stage, pelleted, reground and incorporated into a basal (control) diet of 30% corn + 70% alfalfa by substitution of an appropriate amount of FP for alfalfa. The resulting diets were fed for up to 42 days. The toxic nature of FP was expressed by the following dose-related responses: Fluctuating reductions in feed intake upon initiation of diets containing 35% FP and above; occurrence of a potentially fatal, acute central nervous system syndrome at FP levels as low as 35%; and reduced weight gain at FP levels of 52.5% and above. A wasting condition was observed in 1 ewe fed 52.5% FP. Four of 5 ewes and 1 lamb fed increasing levels of FP developed acute poisoning. One of these ewes developed acute neurotoxicosis when consuming 52.5% FP; 3 other ewes and 1 40-d-old orphaned lamb developed neurotoxicosis upon raising the FP level to 70.0%.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:8470354

  10. Structural and functional characterization of proteinase inhibitors from seeds of Cajanus cajan (cv. ICP 7118).

    PubMed

    Swathi, Marri; Lokya, Vadthya; Swaroop, Vanka; Mallikarjuna, Nalini; Kannan, Monica; Dutta-Gupta, Aparna; Padmasree, Kollipara

    2014-10-01

    Proteinase inhibitors (C11PI) from mature dry seeds of Cajanus cajan (cv. ICP 7118) were purified by chromatography which resulted in 87-fold purification and 7.9% yield. SDS-PAGE, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF/TOF) mass spectrum and two-dimensional (2-D) gel electrophoresis together resolved that C11PI possessed molecular mass of 8385.682 Da and existed as isoinhibitors. However, several of these isoinhibitors exhibited self association tendency to form small oligomers. All the isoinhibitors resolved in Native-PAGE and 2-D gel electrophoresis showed inhibitory activity against bovine pancreatic trypsin and chymotrypsin as well as Achaea janata midgut trypsin-like proteases (AjPs), a devastating pest of castor plant. Partial sequences of isoinhibitor (pI 6.0) obtained from MALDI-TOF/TOF analysis and N-terminal sequencing showed 100% homology to Bowman-Birk Inhibitors (BBIs) of leguminous plants. C11PI showed non-competitive inhibition against trypsin and chymotrypsin. A marginal loss (<15%) in C11PI activity against trypsin at 80 (°)C and basic pH (12.0) was associated with concurrent changes in its far-UV CD spectra. Further, in vitro assays demonstrated that C11PI possessed significant inhibitory potential (IC50 of 78 ng) against AjPs. On the other hand, in vivo leaf coating assays demonstrated that C11PI caused significant mortality rate with concomitant reduction in body weight of both larvae and pupae, prolonged the duration of transition from larva to pupa along with formation of abnormal larval-pupal and pupal-adult intermediates. Being smaller peptides, it is possible to express C11PI in castor to protect them against its devastating pest A. janata. PMID:25093261

  11. Africana Studies 45. . . . . . . . . . . . . . . . . . . American Studies 51. . . . . . . . . . . . . . . . . .

    E-print Network

    Aalberts, Daniel P.

    . . . . . . . . . . Mathematics and Statistics 230. . . . . . . . . . Music 239. . . . . . . . . . Williams in New York 338. . . . . . . . . . . . . . History of the College 3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . The Curriculum 7

  12. Certificate Program AFRICANA STUDIeS

    E-print Network

    Dennehy, John

    spectrum of disciplines including Anthropology, Art, English, Film Studies, History, Music, Political institutions. Among the research resources at their disposal are the New York Public Library, the Mina Rees

  13. College of Arts and Letters Africana Studies

    E-print Network

    Gallo, Linda C.

    Security and Conflict Resolution Center for Latin American Studies Lesbian, Gay, Bisexual & Transgender) School of Nursing School of Social Work Gerontology Social Work o Social Work and Juris Doctorate

  14. A secondary origin of chondrule magnetization in the Allende CV carbonaceous chondrite

    NASA Astrophysics Data System (ADS)

    Carporzen, L.; Fu, R.; Andrade Lima, E.; Weiss, B. P.

    2011-12-01

    Magnetic fields in the solar nebula may have played a key role in the radial transport of angular momentum and mass during the early accretional phase of the solar system. Chondrules and many calcium aluminum inclusions (CAIs), millimeter sized silicate objects found in most chondritic meteorites, were heated to high temperatures and cooled in the nebula and therefore may have recorded a thermoremanent magnetization (TRM) from the nebula field. Additionally, primitive magnetization in chondrules and CAIs may yield constraints about their mode of formation. However, any such primary magnetization may have been significantly altered during subsequent metamorphism and aqueous alteration on the parent asteroid. We performed two tests to determine the nebular origins of remanent magnetization in chondrules and refractory inclusions in the Allende CV3 carbonaceous chondrite: 1) a classic paleomagnetic conglomerate test to identify post-accretional remagnetization events and 2) a unidirectionality test of subsamples taken from individual chondrules and CAIs. We conducted individual measurements of mutually oriented chondrules, CAIs, and matrix as well as SQUID microscope maps of the magnetic fields of 30 ?m thin sections. All samples and thin sections were mutually oriented to within 5°. Our results confirm previous findings that all subsamples of the meteorite carry a unidirectional overprint blocked up to 260°-290°C (MT component). Chondrules and CAIs also carry a higher temperature (HT) remanence oriented in scattered directions unrelated to the direction of the MT overprint. We have confirmed that this HT magnetization is not an artifact of the demagnetization procedure but is a preterrestrial component. Measurements of subsamples of single chondrules and CAIs show that the HT magnetization is not unidirectional within each inclusion. Petrographic data suggests that most magnetic minerals in Allende were the product of parent body alteration. These facts suggest that the magnetization in Allende chondrules and CAIs is not a preaccretional TRM and is unlikely to record the nebular field. We suggest that preaccretional remanence in Allende material was destroyed by aqueous alteration and metasomatism, which was followed or coincident with metamorphism that introduced the MT overprint. The HT component we detect may represent a chemical remanent magnetization (CRM) acquired during aqueous alteration.

  15. Phylogenetic position of Mesorhizobium huakuii subsp. rengei, a symbiont of Astragalus sinicus cv. Japan.

    PubMed

    Nuswantara, S; Fujie, M; Yamada, T; Malek, W; Inaba, M; Kaneko, Y; Murooka, Y

    1999-01-01

    The phylogenetic position of Rhizobium huakuii bv. renge, a symbiont of Astragalus sinicus cv. Japan (renge-sou), was studied. The following phylogenetic approaches were used: restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR) analysis of a full-length 16S rDNA fragment, 16S rDNA analysis of the first 300-bp sequence, bacteriophage typing, and amplification of the genomic region by random primer. All the data suggest that strains of R. huakuii bv. renge should be classified into subspecies of the new genus Mesorhizobium (Jarvis et al., Inter. J. System. Bacteriol., 47, 895-898, 1997) and renamed M. huakuii subsp. rengei. All the strains fell into a tight cluster which included M. loti and M. huakuii. The strains isolated from root nodules on A. sinicus were divided into three groups as follows: group I, M. huakuii subsp. rengei B3, M. huakuii subsp. rengei My6, M. huakuii subsp. rengei My7, M. huakuii subsp. rengei My3, and M. huakuii subsp. rengei OUT30020; group II, M. huakuii subsp. huakuii CCBAU103(T), M. huakuii subsp. huakuii ACCC13005, M. huakuii subsp. huakuii 7653R, and Mesorhizobium sp. N-1; group III, Mesorhizobium sp. OUT30019. All the strains isolated in Japan except strains N-1 and OUT30019 were classified into group I. Strains in group I were sensitive to bacteriophage H1 which was isolated from rice-paddy soil in Japan. Strains in groups II and III except for M. huakuii subsp. huakuii 7653R were resistant to phage H1. Rhizobium sp. ACMP18, a native symbiont of Astragalus cicer that forms nodules on A. sinicus, showed close similarity to M. huakuii subsp. huakuii CCBAU103(T), and should thus be classified as a Mesorhizobium sp. Taken together, the results of the analyses indicate that M. huakuii subsp. rengei forms a subgroup which is distinct from M. huakuii subsp. huakuii strains isolated in China and that strain B3 is the type strain. PMID:16232424

  16. Effects of gamma irradiation on long-storage seeds of Oryza sativa ( cv. 2233) and their surface infecting fungal diversity

    NASA Astrophysics Data System (ADS)

    Maity, J. P.; Kar, S.; Banerjee, S.; Chakraborty, A.; Santra, S. C.

    2009-11-01

    We have reported an effective 60Co gamma irradiation method for the removal and long-time prevention of contaminating fungi of Oryza sativa cv-2233, without the losses of seed viabilities. The fungal growth and their population on gamma treated seeds was found to decrease significantly ( p<0.05) with the increasing irradiation dose and storage time. Immediately after irradiation, the depletion of fungal population on rice seed was noticed (>50%) at 2 kGy, whereas total inhibition was noticed at 3 kGy after 1.5 month.

  17. Chromatic Characteristics and Optically Derived Compositional Descriptors of Micro-oxygenated Wines from Vitis vinifera cv. Merlot and Cabernet Sauvignon

    Microsoft Academic Search

    Sierra Rayne; Stacey Sheppard; Tom Di Bello; Nigel J. Eggers

    2011-01-01

    A 6-month time series of chromatic characteristics and optically derived compositional descriptors was obtained for replicate\\u000a full-scale commercial micro-oxygenated trials and control wines from two varieties, Vitis vinifera cv. Merlot and Cabernet Sauvignon. Two-week post-fermentation micro-oxygenation treatments resulted in a suite of optical\\u000a properties and compositional descriptors significantly different from corresponding replicate non-micro-oxygenated control\\u000a wines. After several months of barrel

  18. Some regulatory aspects of [ 14 C]methylamine influx into Pisum sativum L. cv. Feltham First seedlings

    Microsoft Academic Search

    C. E. Deane-Drummond

    1986-01-01

    [14C]Methylamine influx intoPisum sativum L. cv. Feltham First seedlings showed Michaelis-Menten-type kinetics with apparentVmax=49.2 µmol·g-1 FW·h-1 and apparentKm=0.51 mM. The competitive interactions between ammonium and methylamine were most obvious when biphasic kinetics were assumed with saturation of the first phase at ~0.05 mM. The inhibitor constant for ammonium (Ki)=0.027 mM. When [14C]methylamine was used in trace amounts with ammonium added

  19. Problemas para dormir

    Cancer.gov

    Dormir bien es importante para su salud física y mental. Un buen sueño en la noche no solo le ayuda a pensar claramente, también le baja su presión arterial, ayuda su apetito y fortalece su sistema inmunitario.

  20. Anomalous C-V response correlated to relaxation processes in TiO2 thin film based-metal-insulator-metal capacitor: Effect of titanium and oxygen defects

    NASA Astrophysics Data System (ADS)

    Kahouli, A.; Marichy, C.; Sylvestre, A.; Pinna, N.

    2015-04-01

    Capacitance-voltage (C-V) and capacitance-frequency (C-f) measurements are performed on atomic layer deposited TiO2 thin films with top and bottom Au and Pt electrodes, respectively, over a large temperature and frequency range. A sharp capacitance peak/discontinuity (C-V anomalous) is observed in the C-V characteristics at various temperatures and voltages. It is demonstrated that this phenomenon is directly associated with oxygen vacancies. The C-V peak irreversibility and dissymmetry at the reversal dc voltage are attributed to difference between the Schottky contacts at the metal/TiO2 interfaces. Dielectric analyses reveal two relaxation processes with degeneration of the activation energy. The low trap level of 0.60-0.65 eV is associated with the first ionized oxygen vacancy at low temperature, while the deep trap level of 1.05 eV is associated to the second ionized oxygen vacancy at high temperature. The DC conductivity of the films exhibits a transition temperature at 200 °C, suggesting a transition from a conduction regime governed by ionized oxygen vacancies to one governed by interstitial Ti3+ ions. Both the C-V anomalous and relaxation processes in TiO2 arise from oxygen vacancies, while the conduction mechanism at high temperature is governed by interstitial titanium ions.

  1. [Transformation of embryogenic Calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) mediated by agrobacterium].

    PubMed

    Li, Da-Xu; Zhang, Jie; Zhao, Jian; Zhang, Yi; Li, Li; Liu, Su-Jun; Chen, Fei; Yang, Zhi-Rong

    2006-02-01

    Formation of embryogenic calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) was induced from mature seeds as explants, and proliferated on MS medium containing 2,4-D 5.0 mg/L and KT 0.05 mg/L. An effective and stable callus regeneration system was established by optimizing the culture conditions (Tables 1, 2 and Fig.2). After the calli were subcultured 8 weeks, selected the whitish-yellow-coloured compact nodular calli that transformed with plasmid pCAMBIA1304 carrying hygromycin resistance gene (hptII) and Pseudomonas pseudoalcaligenes insecticidal protein gene (ppIP), which was mediated by an Agrobacterium strain EHA105. Resistant plants were obtained after hygromycin selection (Figs.3, 4). Some important factors that affect the transformation efficiency were studied, which included selection pressure, time of embryogenic calli proliferation, OD value of Agrobacterium suspension, temperature, medium and time of co-cultivation, and concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformation plant regeneration. This research is the first successful genetic transformation of Elymus sibiricus L. cv. Chuancao No.2 mediated by Agrobacterium. PMID:16477130

  2. Interpretation of F106B and CV580 in-flight lightning data and form factor determination

    NASA Technical Reports Server (NTRS)

    Rudolph, T.; Horembala, J.; Eriksen, F. J.; Weigel, H. S.; Elliott, J. R.; Parker, S. L.; Perala, R. A.

    1989-01-01

    Two topics of in-flight aircraft/lightning interaction are addressed. The first is the analysis of measured data from the NASA F106B Thunderstorm Research Aircraft and the CV580 research program run by the FAA and Wright-Patterson Air Force Base. The CV580 data was investigated in a mostly qualitative sense, while the F106B data was subjected to both statistical and quantitative analysis using linear triggered lightning finite difference models. The second main topic is the analysis of field mill data and the calibration of the field mill systems. The calibration of the F106B field mill system was investigated using an improved finite difference model of the aircraft having a spatial resolution of one-quarter meter. The calibration was applied to measured field mill data acquired during the 1985 thunderstorm season. The experimental determination of form factors useful for field mill calibration was also investigated both experimentally and analytically. The experimental effort involved the use of conducting scale models and an electrolytic tank. An analytic technique was developed to aid in the understanding of the experimental results.

  3. Processor core for real time background identification of HD video based on OpenCV Gaussian mixture model algorithm

    NASA Astrophysics Data System (ADS)

    Genovese, Mariangela; Napoli, Ettore

    2013-05-01

    The identification of moving objects is a fundamental step in computer vision processing chains. The development of low cost and lightweight smart cameras steadily increases the request of efficient and high performance circuits able to process high definition video in real time. The paper proposes two processor cores aimed to perform the real time background identification on High Definition (HD, 1920 1080 pixel) video streams. The implemented algorithm is the OpenCV version of the Gaussian Mixture Model (GMM), an high performance probabilistic algorithm for the segmentation of the background that is however computationally intensive and impossible to implement on general purpose CPU with the constraint of real time processing. In the proposed paper, the equations of the OpenCV GMM algorithm are optimized in such a way that a lightweight and low power implementation of the algorithm is obtained. The reported performances are also the result of the use of state of the art truncated binary multipliers and ROM compression techniques for the implementation of the non-linear functions. The first circuit has commercial FPGA devices as a target and provides speed and logic resource occupation that overcome previously proposed implementations. The second circuit is oriented to an ASIC (UMC-90nm) standard cell implementation. Both implementations are able to process more than 60 frames per second in 1080p format, a frame rate compatible with HD television.

  4. In vitro selection and field responses of somaclonal variant plants of rice cv PR113 for drought tolerance

    PubMed Central

    Verma, Dipti; Ansari, Mohammad Wahid; Agrawal, Ganesh Kumar; Rakwal, Randeep; Shukla, Alok; Tuteja, Narendra

    2013-01-01

    Drought is the major environmental stress that limits rice productivity worldwide. In vitro somaclonal variation using different selection agents has been used for crop improvement. Here, rice plants of cv PR113 were selected in vitro on 30, 50 and 70 g L-1 polyethylene glycol 6,000 (PEG). Callus growth, proliferation, calli volume (first and second culture) and plantlet regeneration (third culture) were found to be decreased upto a certain level to acquire tolerance to PEG-induced drought. From the field data, 30 g L-1 PEG lines showed higher vegetative growth (plant height, tiller number, leaf number, shoot weight and root growth) as compared with 50 g L-1 PEG selected somaclone lines under limited irrigation. The yield parameters-panicle length, panicle weight, grains per panicle, 1,000-grain weight, grain yield per plant, harvest index and grain straw ratio were also higher in 30 g L-1 PEG lines as compared with 50 g L-1 PEG lines. The results, therefore indicate that 30 g L-1 PEG selected somaclone lines were more suited than 50 g L-1 PEG selected somaclone lines under stress as compared with WT. The finding suggests that rice cv PR113 somaclones generated on PEG are found to be drought tolerant under field condition with better yield. PMID:23333982

  5. Preharvest salicylic acid treatments to improve quality and postharvest life of table grapes (Vitis vinifera L.) cv. Flame Seedless.

    PubMed

    Champa, W A Harindra; Gill, M I S; Mahajan, B V C; Arora, N K

    2015-06-01

    Significance of preharvest salicylic acid (SA) treatments on maturity, quality and postharvest life of grape cv. Flame Seedless were studied during two years. The experiment was performed on 12-year old own rooted, grapevines planted at 3 m?×?3 m spacing trained on overhead system. Vines were treated with aqueous solutions of SA (0.0, 1.0, 1.5 and 2.0 mM) at pea stage and at veraison. After harvesting, clusters were divided into two lots in which one was subjected to initial quality evaluation, while the other was stored in cold room (3-4 °C, 90-95 % RH) for evaluation of postharvest quality. SA at the dose of 1.5 and 2.0 mM hastened berry maturity by 3 to 5 days, produced less compact bunches alongside larger berries in contrast to control and the lowest dose. The same doses effectively maintained peel colour, higher firmness, lower pectin methyl esterase activity and electrolyte leakage alongside suppressing degradation of TSS and TA during cold storage. These two doses also exhibited higher efficacy on maintaining anthocyanins, phenols and organoleptic properties while reducing weight loss, rachis browning and decay incidence. Correlation analysis demonstrated that many quality parameters are interdependent. In conclusion, preharvest spray of 1.5 mM SA proved to be an effective means of improving quality and extending postharvest life of grape cv. Flame Seedless. PMID:26028743

  6. Application of Five Light-Response Models in the Photosynthesis of Populus?×?Euramericana cv. 'Zhonglin46' Leaves.

    PubMed

    Fang, Lidong; Zhang, Shuyong; Zhang, Guangcan; Liu, Xia; Xia, Xuanxuan; Zhang, Songsong; Xing, Wei; Fang, Xiaochen

    2015-05-01

    The light-response curve of photosynthesis is an important tool used to study plant ecophysiology and can provide a scientific basis for the response of plant photosynthetic characteristics to environmental factors. At present, there are five common light-response models of photosynthesis. To gain deeper insight into the applicability of different light-response models of photosynthesis and the photosynthetic physiological characteristics of Populus euramericana cv. 'Zhonglin46', two typical light-response curves of photosynthesis in P. euramericana cv. 'Zhonglin46' leaves, one under drought stress and the other under control conditions, were measured using a CIRAS-2 portable photosynthesis system. The light-response data were divided into two groups: one set of data was used to fit light-response curves, and the other set of data was used to test them. The accuracy of the fitting and the predictions of the different models were evaluated by mean square error and mean absolute error. The results showed that the light-response curves of P. euramericana cv. 'Zhonglin46' under drought stress matched the light-saturated inhibition type and that those under the control condition matched the approaching light-saturation type. The two new models (i.e., the modified rectangular hyperbola model and modified exponential model) fit the two light-response curves and their characteristic parameters well, and the fitting results of the two models were similar. Conversely, the three traditional models (i.e., the rectangular hyperbola model, nonrectangular hyperbola model, and exponential model) did not fit the two light-response curves well; in particular, they overestimated the maximum net photosynthetic rate, underestimated the light saturation point (LSP), and did not fit the net photosynthetic rate during the light-saturated stage. The LSP calculated by the "linear method" combined with the traditional models was significantly lower than the measured values; additionally, the appropriate value of the proportional coefficient was difficult to determine, and the assumed value (empirical value) could easily lead to unreliable results by the "coefficient method". The "estimation method" based on the measured light-response data was still a relatively accurate, simple, and practical way to determine LSP. In addition, the nonrectangular hyperbolic model also had good accuracy and applicability in fitting the approaching light saturation curve on the basis of the "estimation method" to determine LSP. PMID:25832179

  7. The Anatomy and Bulk Composition of CAI Rims in the Vigarano (CV3) Chondrite

    NASA Astrophysics Data System (ADS)

    Ruzicka, A.; Boynton, W. V.

    1993-07-01

    A striking feature of Ca,Al-rich inclusions (CAIs) in chondrites is the presence of mineralogical layers that typically form rim sequences up to 50 micrometers thick [1]. Many ideas regarding the origin of CAI rims have been proposed, but none are entirely satisfactory. The detailed mineralogy and bulk compositions of relatively unaltered CAI rims in the Vigarano (CV3) chondrite described here provide constraints on hypotheses of rim formation. Rim Mineralogy: CAIs in Vigarano consist of melilite (mel)- and spinel (sp)- rich varieties, both of which are rimmed [2]. Around mel-rich objects, the layer sequence is CAI interior --> sp-rich layer (sometimes absent) --> mel/anorthite (anor) layer --> Ti-Al-rich clinopyroxene (Tpx) layer --> Al- diopside (Al-diop) layer --> olivine (ol) +/- Al-diop layer --> host matrix. The sequence around sp-rich objects differs from this in that the mel/anor layer is absent. Both the sp-rich layer around mel-cored CAIs and the cores of sp-rich CAIs in Vigarano are largely comprised of a fine-grained (<=1 micrometer) intergrowth of sp, Tpx, and minor mel and perovskite. These intergrowths are typically so fine grained that little internal texture is discernible. Mixing calculations suggest the presence of ~10 vol% Tpx in the sp-rich layer of two mel-cored CAIs, and the presence of ~35 vol% Tpx within one sp-cored CAI. The mel/anor layer is sometimes monomineralic, consisting of mel alone, or bimineralic, consisting of both mel and anor. Where bimineralic, anor typically occurs in the outer part of the layer. In places, anor (An(sub)99-100) has partially altered to nepheline and voids. Rim mel is systematically less gehlenitic than mel in the CAI interiors, especially compared to mel in the interior adjacent to the rims. The Tpx layer (>2 and up to 15 wt% TiO2) and Al-diop layer (<2 wt% TiO2) are monomineralic and show chemical zoning trends radial to the CAIs. Moving outward, TiO2 and Al2O3 generally decrease, while SiO2 and MgO increase, although Al2O3 shows a small concentration maximum in the Al-diop layer. High-quality EMPA data suggest that Ti^3+/Ti^4+ decreases outward in the Tpx layer, and that Fe^3+ is present in the Al-diop layer, implying that a steep gradient in oxidation state occurs across these two layers. The ol layer is comprised of individually zoned grains (<=5 micrometers across) that have forsteritic cores and thin (<=1 micrometer) rims of more ferrous ol. The ol grains often form triple-grain junctions and occasionally form clusters that are enclosed by Al-diop. The texture of this polycrystalline layer suggests that it formed by the attachment of preexisting ol grains onto the surfaces of CAIs, and the triple-junctions and steep zoning profiles of the ol grains suggest that they were annealed in a short-lived heating event. Bulk Rim Composition: Bulk rim compositions for several mel-rich CAIs were determined by using EMPA traverses across representative portions of the rims. These compositions plot within the sp + forsterite (fo) field of the gehlenite-anorthite-forsterite ternary diagram of Stolper [3], and are unattainable by the igneous crystallization of a mel-rich CAI composition. Moreover, a hypothetical melt with the composition of the rims has a predicted crystallization sequence (sp --> sp + fo --> sp + fo + anor or mel or Tpx) that does not correspond to observed rim sequences. It thus appears that (1) the rim region did not form through crystallization of molten CAIs; and (2) rim layers did not originate solely by the crystallization of a melt layer present on a solid CAI core [4,5]. References: [1] Wark D. A. and Lovering J. F. (1977) Proc. LSC 8th, 95-112. [2] Ruzicka A. and Boynton W. V. (1991) Meteoritics, 26, 390-391. [3] Stolper E. (1982) GCA, 46, 2159-2180. [4] Korina M. I. et al. (1982) LPS XIII, 399- 400. [5] Bunch T. E. and Chang S. (1980) Meteoritics, 15, 270- 271.

  8. 53Mn-53Cr dating of fayalite formation in the CV3 chondrite Mokoia: evidence for asteroidal alteration.

    PubMed

    Hutcheon, I D; Krot, A N; Keil, K; Phinney, D L; Scott, E R

    1998-12-01

    Fayalite grains in chondrules in the oxidized, aqueously altered CV3 chondrite Mokoia have large excesses of radiogenic chromium-53. These excesses indicate the in situ decay of short-lived manganese-53 (half-life = 3.7 million years) and define an initial 53Mn/55Mn ratio of 2.32 (+/-0.18) x 10(-6). This ratio is comparable to values for carbonates in CI and CM chondrites and for several classes of differentiated meteorites. Mokoia fayalites formed 7 to 16 million years after Allende calcium-aluminum-rich inclusions, during hydrothermal activity on a geologically active asteroid after chondritic components had ceased forming in the solar nebula. PMID:9836631

  9. Complete genome sequence of a banana bract mosaic virus isolate infecting the French plantain cv. Nendran in India.

    PubMed

    Balasubramanian, V; Selvarajan, R

    2012-02-01

    The first complete genome sequence of an Indian isolate (TRY) of Banana bract mosaic virus (BBrMV) was determined following virus RNA extraction from the French plantain cv. Nendran (AAB). The complete genome was 9711 nucleotides excluding the poly(A) tail and had a genome organization similar to that of a Philippine (PHI) isolate characterized earlier. When compared to BBrMV-PHI, the complete genome sequence of BBrMV-TRY was 94% identical at the nucleotide level and its ten mature proteins had amino acid sequence identities ranging from 88 to 98%. Phylogenetic analysis suggests that the BBrMV-TRY isolate is closely related to the BBrMV-PHI isolate. PMID:22134527

  10. Physiological and biochemical changes associated with flower development and senescence in so far unexplored Helleborus orientalis Lam. cv. Olympicus.

    PubMed

    Shahri, Waseem; Tahir, Inayatullah; Islam, Sheikh Tajamul; Bhat, Mushtaq Ahmad

    2011-03-01

    The so far unexplored H. Orientalis cv. Olympicus exhibits a unique pattern of flower senescence, involving re-greening of creamy white petaloid sepals at the later stages. The greenish sepals become photosynthetically competent immediately after pollination and persist until the seeds are set. After the seed set, the entire (green) flower abscises from the plant. Flower development of Helleborus orientalis cv. Olympicus growing in the open was divided into six stages (I-VI) from tight bud stage to the senescent stage. The average life span of an individual flower after it is fully open is about 6 days. Membrane permeability of sepal tissues estimated as electrical conductivity of leachates increased during senescence. The content of sugars and soluble proteins in the sepal tissues increased during flower opening and declined thereafter during senescence. The protease activity increased as the flower progressed towards senescence. From the present study, it becomes evident that decline in the sugar status and elevation in specific protease activity leading to degradation of proteins are the important factors regulating development and senescence in H. orientalis flowers. Although the tissue content of soluble proteins registered an overall quantitative decrease but SDS-PAGE of protein extract from sepal tissues suggested a decrease in the expression of high molecular weight proteins and an increase in low molecular weight proteins during flower development and senescence. At this stage it is not known whether the polypeptides that increased during senescence play an important role in the senescence of Helleborus orientalis flowers. The increase in these polypeptides during flower senescence is of particular interest because they may be linked to flower longevity. Understanding the nature of these proteins can provide new insights into the pathways that execute senescence and the post-transcriptional regulation of senescence in this flower system. PMID:23572993

  11. Draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi).

    PubMed

    Kolton, Max; Green, Stefan J; Harel, Yael Meller; Sela, Noa; Elad, Yigal; Cytryn, Eddie

    2012-10-01

    Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

  12. Survey of 42,000 Gossypium hirsutum cv. Maxxa BAC-End Sequences and Frequency, Type, and Annotation of BAC-derived SSRs.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The quest for more molecular markers is a major initiative in cotton, which lags behind crops such as soybean, maize, and rice in this type of research. In an effort to increase the number of microsatellite markers in Gossypium, BAC-end sequences from a publicly available Gossypium hirsutum cv. Maxx...

  13. The Hewlett-Packard HP-41CV Hand-Held Computer as a Medium for Teaching Mathematics to Fire Control Systems Repairers. Research Report 1408.

    ERIC Educational Resources Information Center

    Boldovici, John A.; Scott, Thomas D.

    A study compared the benefits of using the Hewlett-Packard HP-41CV hand-held computer, as opposed to conventional training without computers, in teaching mathematics to fire control systems repairers. Thirty soldiers in a course to train fire control systems repairers received training in technical mathematics using the hand-held computer, whereas…

  14. Calculating inverse cv, skew and pwm functions for pearson3, log-normal, extreme-value and log-logistic distributions

    Microsoft Academic Search

    R. W. Donaldson

    1996-01-01

    Equations are presented to calculate inverse CV, skew and PWM functions for the Pearson-3, log-normal, extreme-value and log-logistic distributions. Such inverse functions are used for moment and PWM estimates. Close numerical approximations are derived for the inverse functions that do not exist explicitly. This is intended to overcome the intractable nature of moment and PWM estimates.

  15. USE OF REPEATED BRONCHOALVEOLAR LAVAGE IN RABBITS TO ASSESS POLLUTANT-INDUCED LUNG CHANGES IN AN ANIMAL MODEL OF CARDIOVASCULAR (CV) DISEASE.

    EPA Science Inventory

    Animal models of coronary heart disease (e.g., hyperlipidemic rabbits) are being used to investigate epidemiologic associations between higher levels of air pollution and adverse CV consequences. Mechanisms by which pollutant-induced lung or systemic inflammation leads to acute C...

  16. Phytochemicals and antioxidant capacity of orange ( Citrus sinensis (l.) Osbeck cv. Salustiana) juice produced under organic and integrated farming system in Greece

    Microsoft Academic Search

    Peter A. Roussos

    2011-01-01

    Organically and integrated produced orange (Citrus sinensis (l.) Osbeck cv. Salustiana) fruits were assayed in terms of fruit characteristics and juice phytochemicals over a period of two years. Fruit size and juice volume were higher under organic farming system. There were not any significant differences concerning either the carbohydrates’ or organic acids’ concentrations of the juice. Similar results were obtained

  17. Free radical scavenging activity and phenolic content in achenes and thalamus from Fragaria chiloensis ssp. chiloensis, F. vesca and F. x ananassa cv. Chandler

    Microsoft Academic Search

    José Cheel; Cristina Theoduloz; Jaime A. Rodríguez; Peter D. S. Caligari; Guillermo Schmeda-Hirschmann

    2007-01-01

    The total phenolic, flavonoid and anthocyanin content of achenes (true fruit) and thalamus (receptacle) from the native South American Fragaria chiloensis ssp. chiloensis (f. patagonica and f. chiloensis), Fragaria vesca and Fragaria x ananassa cv. Chandler was determined by spectrophotometric means. Highest phenolic content was found in F. vesca while lowest content was measured for white strawberry (F. chiloensis ssp.

  18. Dietary advice combined with a salicylate, mineral and vitamin supplement (CV247) has some tumour static properties: a phase II study

    Microsoft Academic Search

    Robert Thomas; Mabel Blades; Madeleine Williams; Sara Godward

    2005-01-01

    Purpose – This pilot study seeks to evaluate whether dietary intervention combined with an oral formulation of sodium salicylate, mineral and vitamin supplements (CV247) altered the rate of progression in patients with malignant disease. Design\\/methodology\\/approach – A total of 37 patients were recruited from March 2001 to November 2001 and ran to September 2004. All patients had progressive malignancy at

  19. The effects of fertilizer rate on vegetative growth, yield and fruit quality, with special respect to pigments, in black chokeberry ( Aronia melanocarpa) cv. `Viking'

    Microsoft Academic Search

    Niklas Jeppsson

    2000-01-01

    The effect of fertilizer rate on fruit quality parameters in the black chokeberry (Aronia melanocarpa [Michx] Ell.) cv. `Viking' was studied over three years. A combined N–P–K fertilizer was applied in different rates. Plant height, yield, berry weight, anthocyanin content, content of brown compounds, content of soluble solids, anthocyanin composition and total acidity were recorded for the different treatments. Increased

  20. Effects of Vaccination with 10-Valent Pneumococcal Non-Typeable Haemophilus influenza Protein D Conjugate Vaccine (PHiD-CV) on the Nasopharyngeal Microbiome of Kenyan Toddlers

    PubMed Central

    Feazel, Leah M.; Santorico, Stephanie A.; Robertson, Charles E.; Bashraheil, Mahfudh; Scott, J. Anthony G.

    2015-01-01

    Objective Pneumococcal conjugate vaccines reduce the prevalence of vaccine serotypes carried in the nasopharynx. Because this could alter carriage of other potential pathogens, we assessed the nasopharyngeal microbiome of children who had been vaccinated with 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). Methods Profiles of the nasopharyngeal microbiota of 60 children aged 12-59 months, who had been randomized to receive 2 doses of PHiD-CV (n=30) or Hepatitis A vaccine (n=30) 60 days apart, were constructed by 16S rRNA gene pyrosequencing of swab specimens collected before vaccination and 180 days after dose 1. Results Prior to vaccination, Moraxella catarrhalis (median of 12.3% of sequences/subject), Streptococcus pneumoniae (4.4%) and Corynebacterium spp. (5.6%) were the most abundant nasopharyngeal bacterial species. Vaccination with PHiD-CV did not significantly alter the species composition, abundance, or prevalence of known pathogens. Distinct microbiomes were identified based on the abundances of Streptococcus, Moraxella, and Haemophilus species. These microbiomes shifted in composition over the study period and were independent of age, sex, school attendance, antibiotic exposure, and vaccination. Conclusions Vaccination of children with two doses of PHiD-CV did not significantly alter the nasopharyngeal microbiome. This suggests limited replacement carriage with pathogens other than non-vaccine strains of S. pneumoniae. Trial Registration clinicaltrials.gov NCT01028326 PMID:26083474

  1. A new immuno- dystrophin-deficient model, the NSG-mdx4Cv mouse, provides evidence for functional improvement following allogeneic satellite cell transplantation

    PubMed Central

    Arpke, Robert W.; Darabi, Radbod; Mader, Tara L.; Zhang, Yu; Toyama, Akira; Lonetree, Cara-lin; Nash, Nardina; Lowe, Dawn A.; Perlingeiro, Rita C.R.; Kyba, Michael

    2013-01-01

    Transplantation of a myogenic cell population into an immunodeficient recipient is an excellent way of assessing the in vivo muscle-generating capacity of that cell population. To facilitate both allogeneic and xenogeneic transplantations of muscle-forming cells in mice we have developed a novel immunodeficient muscular dystrophy model, the NSG-mdx4Cv mouse. The IL2Rg mutation, which is linked to the Dmd gene on the X chromosome, simultaneously depletes NK cells and suppresses thymic lymphomas, issues that limit the utility of the SCID/mdx model. The NSG-mdx4Cv mouse presents a muscular dystrophy of similar severity to the conventional mdx mouse. We show that this animal supports robust engraftment of both pig and dog muscle mononuclear cells. The question of whether satellite cells prospectively isolated by flow cytometry can confer a functional benefit upon transplantation has been controversial. Using allogeneic Pax7-ZsGreen donors and NSG-mdx4Cv recipients, we demonstrate definitively that as few as 900 FACS-isolated satellite cells can provide functional regeneration in vivo, in the form of an increased mean maximal force-generation capacity in cell-transplanted muscles, compared to a sham-injected control group. These studies highlight the potency of satellite cells to improve muscle function, and the utility of the NSG-mdx4Cv model for studies on muscle regeneration and Duchenne muscular dystrophy therapy. PMID:23606600

  2. Identification and quantification of phenols, carotenoids, and vitamin C from papaya ( Carica papaya L., cv. Maradol) fruit determined by HPLC-DAD-MS\\/MS-ESI

    Microsoft Academic Search

    Laura E. Gayosso-García Sancho; Elhadi M. Yahia; Gustavo Adolfo González-Aguilar

    2011-01-01

    Recent studies have demonstrated that vitamin C, phenols, and carotenoids are bioactive compounds that protect the body from oxidative stress, reducing the risk of cardiovascular diseases and some types of cancer. Qualitative and quantitative analysis of the major phytochemicals found in papaya fruit flesh and skin (Carica papaya L., cv Maradol) was conducted in four stages of ripeness, using high-performance

  3. Onco-neural antibodies and tumour type determine survival and neurological symptoms in paraneoplastic neurological syndromes with Hu or CV2/CRMP5

    E-print Network

    Boyer, Edmond

    Onco-neural antibodies and tumour type determine survival and neurological symptoms in paraneoplastic neurological syndromes with Hu or CV2/CRMP5 antibodies Honnorat J r meé ô 1 2 * , Cartalat of Psychiatry7 Ohio State University, Columbus, Ohio,US Service of Neurology8 Universitat de Barcelona, Institut

  4. Case 2:08-cv-05093-HB-DCF Document 263 Filed 02/14/14 Page 1 of 1 10COHEN MILSTEIN

    E-print Network

    Straight, Aaron

    Case 2:08-cv-05093-HB-DCF Document 263 Filed 02/14/14 Page 1 of 1 10COHEN MILSTEIN Joel Laitman. Respectfully submitted, /s/ Joel P. Laitman /s/ Thomas C. Rice Joel P. Laitman Thomas C. Rice Counsel for Plaintiffs Counsel for Defendants Cohen Milstein Sellers & Toll PLLC 88 Pine Street 14th Floor New York, NY

  5. Case 1:09-cv-01575-RCL Document 48 Filed 08/31/10 Page 1 of 2 IN THE UNITED STATES DISTRICT COURT

    E-print Network

    Bandettini, Peter A.

    . United States Attorney SHEILA LIEBER Deputy Branch Director, Federal Programs Branch /s/ Kyle R. Freeny KYLE R. FREENY, CA Bar No. 247857 Trial Attorneys, Federal Programs Branch U.S. Department of Justice-8470 Email: Kyle.Freeny@usdoj.gov Counsel for Defendants -2 #12;Case 1:09-cv-01575-RCL Document 48-1 Filed 08

  6. A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV Hollot2, J Horowitz3, and RP Shrestha4, Y Chait5

    E-print Network

    Massachusetts at Amherst, University of

    A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV on performance of anemia management protocols. · Anemia of end-stage kidney disease (ESRD) is characterized-specific gains should play a role in their design. · The interaction of anemia management protocols (AMPs

  7. Tissue culture in synthetic atmospheres: diffusion rate effects on cytokinin-induced callus growth and isoflavonoid production in soybean [ Glycine max (L.) Merr. cv. Acme

    Microsoft Academic Search

    Lindsey K. Tuominen; Mary E. Musgrave

    2006-01-01

    Concentration is one factor that is known to determine how metabolic gases influence the growth and secondary metabolism of plant tissues in culture. How actual gas bioavailability influences these processes has not been studied despite its potential importance in specialized applications. A simple model system, soybean [Glycine max (L.) Merr. cv. Acme] callus culture, was selected for experiments because exogenous

  8. Draft Genome Sequence of Flavobacterium sp. Strain F52, Isolated from the Rhizosphere of Bell Pepper (Capsicum annuum L. cv. Maccabi)

    PubMed Central

    Kolton, Max; Green, Stefan J.; Harel, Yael Meller; Sela, Noa; Elad, Yigal

    2012-01-01

    Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

  9. Study on the Nutritional Characteristics of Baxi Banana (Musa AAA Cavendish cv. Baxi) and Application Technique of Potassium and Magnesium Fertilizer

    Microsoft Academic Search

    Yao Lixian; Zhou Xiuchong; Li Guoliang; Peng Zhiping

    It is important to study the combined application of potassium and magnesium fertilizer in banana fertilization. This paper investigated the nutritional characteristics of Baxi banana (Musa AAA Cavendish cv. Baxi) and effect of combined application of potassium and magnesium fertilizer on its growth. Results from field trials indicated that stem and leaf were the main parts for nutrient accumulation. During

  10. Improving the french fry quality of russeted potatoes through transformation with the anti-sweetening gene (UgpA) from the Chipping cv. Snowden

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Microtubers of two dual-purpose russeted potatoes were transformed with the anti-sweetening gene (UgpA) from the cv. Snowden using well know Agrobacterium tumifaciens mediated transformation system. Seventy-two and twenty-four distinct transformants of AOND95292-3Russ and ND7882b-7Russ, respectivel...

  11. Effect of potassium on drought resistance of Hibiscus rosa-sinensis cv. Leprechaun: Plant growth, leaf macro- and micronutrient content and root longevity

    Microsoft Academic Search

    Jonathan N. Egilla; Fred T. Davies; Malcolm C. Drew

    2001-01-01

    As competition for the limited water supply available for irrigation of horticultural crops increases, research into crop management practices that enhance drought resistance, plant water-use efficiency and plant growth when water supply is limited has become increasingly essential. This experiment was conducted to determine the effect of potassium (K) nutritional status on the drought resistance of Hibiscus rosa-sinensis L. cv.

  12. Efectos de extractos acuosos de la maleza Cyperus rotundus L. (Cyperaceae) sobre la germinación de semillas y crecimiento de plántulas de maíz (Zea mays L.) cv. Pioneer 3031

    Microsoft Academic Search

    José A. Laynez-Garsaball; Jesús Rafael Méndez-Natera

    The objective of this experiment was to determine the effects of foliage water extracts of purple nutsedge (Cyperus rotundus L.) on seed germination and seedling growth of corn ( Cyperus rotundus L.) on seed germination and seedling growth of corn ( Cyperus rotundus Zea mays L.) cv. Pioneer 3031. An extract was prepared with nutsedge foliage of 15 days at

  13. A Novel Objective Method of Estimating the Age of Mandibles from African Elephants (Loxodonta africana Africana).

    PubMed

    Stansfield, Fiona J

    2015-01-01

    The importance of assigning an accurate estimate of age and sex to elephant carcasses found in the wild has increased in recent years with the escalation in levels of poaching throughout Africa. Irregularities identified in current ageing techniques prompted the development of a new method to describe molar progression throughout life. Elephant mandibles (n = 323) were studied and a point near the distal dental alveolus was identified as being most useful in ranking each jaw according to molar progression. These 'Age Reference Lines' were then associated with an age scale based on previous studies and Zimbabwean mandibles of known age. The new ranking produced a single age scale that proved useful for both male and female mandibles up to the maximum lifespan age of 70-75 years. Methods to aid in molar identification and the sexing of found jaws were also identified. PMID:25970428

  14. A Novel Objective Method of Estimating the Age of Mandibles from African Elephants (Loxodonta africana Africana)

    PubMed Central

    Stansfield, Fiona J.

    2015-01-01

    The importance of assigning an accurate estimate of age and sex to elephant carcasses found in the wild has increased in recent years with the escalation in levels of poaching throughout Africa. Irregularities identified in current ageing techniques prompted the development of a new method to describe molar progression throughout life. Elephant mandibles (n = 323) were studied and a point near the distal dental alveolus was identified as being most useful in ranking each jaw according to molar progression. These ‘Age Reference Lines’ were then associated with an age scale based on previous studies and Zimbabwean mandibles of known age. The new ranking produced a single age scale that proved useful for both male and female mandibles up to the maximum lifespan age of 70–75 years. Methods to aid in molar identification and the sexing of found jaws were also identified. PMID:25970428

  15. Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability

    PubMed Central

    2013-01-01

    Background Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation. Reviewer This article was reviewed by Sándor Pongor, L. Aravind and Anthony M. Poole. PMID:23324115

  16. Software para generación de cuestionarios

    Microsoft Academic Search

    Banco Interamericano de Desarrollo

    2008-01-01

    La presentación muestra el sistema informático completo y los procesos utilizados por el Departamento Administrativo Nacional de Estadística de Colombia para el relevamiento y procesamiento de datos y para la generación de información. Luego, se describe en detalle y a través de capturas de pantalla las características y funcionalidades del software para la gestión de los datos (GEA) que permite

  17. Characterization of the adaptive response of grapevine (cv. Tempranillo) to UV-B radiation under water deficit conditions.

    PubMed

    Martínez-Lüscher, J; Morales, F; Delrot, S; Sánchez-Díaz, M; Gomès, E; Aguirreolea, J; Pascual, I

    2015-03-01

    This work aims to characterize the physiological response of grapevine (Vitis vinifera L.) cv. Tempranillo to UV-B radiation under water deficit conditions. Grapevine fruit-bearing cuttings were exposed to three levels of supplemental biologically effective UV-B radiation (0, 5.98 and 9.66kJm(-2)day(-1)) and two water regimes (well watered and water deficit), in a factorial design, from fruit-set to maturity under glasshouse-controlled conditions. UV-B induced a transient decrease in net photosynthesis (Anet), actual and maximum potential efficiency of photosystem II, particularly on well watered plants. Methanol extractable UV-B absorbing compounds (MEUVAC) concentration and superoxide dismutase activity increased with UV-B. Water deficit effected decrease in Anet and stomatal conductance, and did not change non-photochemical quenching and the de-epoxidation state of xanthophylls, dark respiration and photorespiration being alternative ways to dissipate the excess of energy. Little interactive effects between UV-B and drought were detected on photosynthesis performance, where the impact of UV-B was overshadowed by the effects of water deficit. Grape berry ripening was strongly delayed when UV-B and water deficit were applied in combination. In summary, deficit irrigation did not modify the adaptive response of grapevine to UV-B, through the accumulation of MEUVAC. However, combined treatments caused additive effects on berry ripening. PMID:25617319

  18. Recessive Resistance Derived from Tomato cv. Tyking-Limits Drastically the Spread of Tomato Yellow Leaf Curl Virus.

    PubMed

    Pereira-Carvalho, Rita C; Díaz-Pendón, Juan A; Fonseca, Maria Esther N; Boiteux, Leonardo S; Fernández-Muñoz, Rafael; Moriones, Enrique; Resende, Renato O

    2015-05-01

    The tomato yellow leaf curl disease (TYLCD) causes severe damage to tomato (Solanum lycopersicum L.) crops throughout tropical and subtropical regions of the world. TYLCD is associated with a complex of single-stranded circular DNA plant viruses of the genus Begomovirus (family Geminiviridae) transmitted by the whitefy Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae). The tomato inbred line TX 468-RG is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. Tyking F1. A detailed analysis of this germplasm source against tomato yellow leaf curl virus-Israel (TYLCV-IL), a widespread TYLCD-associated virus, showed a significant restriction to systemic virus accumulation even under continuous virus supply. The resistance was effective in limiting the onset of TYLCV-IL in tomato, as significantly lower primary spread of the virus occurred in resistant plants. Also, even if a limited number of resistant plants could result infected, they were less efficient virus sources for secondary spread owing to the impaired TYLCV-IL accumulation. Therefore, the incorporation of this resistance into breeding programs might help TYLCD management by drastically limiting TYLCV-IL spread. PMID:26008699

  19. Effect of salicylhydroxamic acid on endosperm strength and embryo growth of Lactuca sativa L. cv Waldmann's Green seeds

    NASA Technical Reports Server (NTRS)

    Brooks, C. A.; Mitchell, C. A.

    1988-01-01

    Salicylhydroxamic acid (SHAM) stimulated germination of photosensitive lettuce (Lactuca sativa L. cv Waldmann's Green) seeds in darkness. To determine whether SHAM acts on the embryo or the endosperm, we investigated separately effects of SHAM on growth potential of isolated embryos as well as on endosperm strength. Embryo growth potential was quantified by incubating decoated embryos in various concentrations of osmoticum and measuring subsequent radicle elongation. Growth potential of embryos isolated from seeds pretreated with 4 millimolar SHAM was equal to that of untreated controls. Rupture strength of endosperm tissue excised from seeds pretreated with SHAM was 33% less than that of controls in the micropylar region. To determine if the embryo must be in contact with the endosperm of SHAM to weaken the endosperm, some endosperms were incubated with SHAM only after dissection from seeds. Rupture strength of SHAM-treated, isolated endosperms in the micropylar region was 25% less than that of untreated controls. There was no difference in rupture strength in the cotyledonary region of endosperm isolated from seeds treated with SHAM in buffer or buffer alone. SHAM therefore stimulates germination not by enhancing embryo growth potential, but by weakening the micropylar region of the endosperm enclosing the embryo.

  20. Recessive Resistance Derived from Tomato cv. Tyking-Limits Drastically the Spread of Tomato Yellow Leaf Curl Virus

    PubMed Central

    Pereira-Carvalho, Rita C.; Díaz-Pendón, Juan A.; Fonseca, Maria Esther N.; Boiteux, Leonardo S.; Fernández-Muñoz, Rafael; Moriones, Enrique; Resende, Renato O.

    2015-01-01

    The tomato yellow leaf curl disease (TYLCD) causes severe damage to tomato (Solanum lycopersicum L.) crops throughout tropical and subtropical regions of the world. TYLCD is associated with a complex of single-stranded circular DNA plant viruses of the genus Begomovirus (family Geminiviridae) transmitted by the whitefy Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae). The tomato inbred line TX 468-RG is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. Tyking F1. A detailed analysis of this germplasm source against tomato yellow leaf curl virus-Israel (TYLCV-IL), a widespread TYLCD-associated virus, showed a significant restriction to systemic virus accumulation even under continuous virus supply. The resistance was effective in limiting the onset of TYLCV-IL in tomato, as significantly lower primary spread of the virus occurred in resistant plants. Also, even if a limited number of resistant plants could result infected, they were less efficient virus sources for secondary spread owing to the impaired TYLCV-IL accumulation. Therefore, the incorporation of this resistance into breeding programs might help TYLCD management by drastically limiting TYLCV-IL spread. PMID:26008699

  1. Nitrogen balance for wheat canopies (Triticum aestivum cv. Veery 10) grown under elevated and ambient CO2 concentrations

    NASA Technical Reports Server (NTRS)

    Smart, D. R.; Ritchie, K.; Bloom, A. J.; Bugbee, B. B.

    1998-01-01

    We examined the hypothesis that elevated CO2 concentration would increase NO3- absorption and assimilation using intact wheat canopies (Triticum aestivum cv. Veery 10). Nitrate consumption, the sum of plant absorption and nitrogen loss, was continuously monitored for 23 d following germination under two CO2 concentrations (360 and 1000 micromol mol-1 CO2) and two root zone NO3- concentrations (100 and 1000 mmol m3 NO3-). The plants were grown at high density (1780 m-2) in a 28 m3 controlled environment chamber using solution culture techniques. Wheat responded to 1000 micromol mol-1 CO2 by increasing carbon allocation to root biomass production. Elevated CO2 also increased root zone NO3- consumption, but most of this increase did not result in higher biomass nitrogen. Rather, nitrogen loss accounted for the greatest part of the difference in NO3- consumption between the elevated and ambient [CO2] treatments. The total amount of NO3(-)-N absorbed by roots or the amount of NO3(-)-N assimilated per unit area did not significantly differ between elevated and ambient [CO2] treatments. Instead, specific leaf organic nitrogen content declined, and NO3- accumulated in canopies growing under 1000 micromol mol-1 CO2. Our results indicated that 1000 micromol mol-1 CO2 diminished NO3- assimilation. If NO3- assimilation were impaired by high [CO2], then this offers an explanation for why organic nitrogen contents are often observed to decline in elevated [CO2] environments.

  2. Response of different maturity stages of sapota (Manilkara achras Mill.) cv. Kallipatti to in-package ethylene absorbent.

    PubMed

    Bhutia, Wangdup; Pal, R K; Sen, Sangita; Jha, S K

    2011-12-01

    Sapota fruits are highly perishable due to their climacteric nature. The rapid softening of fruits is primarily due to high activity of many oxidative enzymes and liberation of ethylene. Harvest maturity plays a crucial role in deciding the marketability of climacteric fruits in general. Attempt has been made to evaluate the response of ethylene absorbent on variable maturity groups of harvested Sapota cv. Kallipatti with the objective to delay the ripening during transit and extend its marketability during storage at ambient condition (27-32 °C & 65-75% R.H.). Harvested fruits having three different degree of ripeness (as maturity indices viz. mature, half-ripe and ripe) were packed with or without ethylene absorbent sachets (Bioconservación, France) in 10 kg CFB boxes and transported from Dahnu to Delhi covering a distant of approximately 2500 KM by truck on road along with conventional packaging as control. The fruits were evaluated immediately on arrival at Delhi and subsequently during storage for various physical, physiological, biochemical and decay parameters. Mature fruits with ethylene absorbent exhibited maximum delay in ripening, low ethylene liberation, weight loss and high fruit firmness. The response of ethylene absorbent to extend the marketability of ripe fruit was not significant. PMID:23572819

  3. James Webb Space Telescope (JWST) Integrated Science Instruments Module (ISIM) Cryo-Vacuum (CV) Test at GSFC

    NASA Technical Reports Server (NTRS)

    Yew, Calinda M.

    2014-01-01

    JWST ISIM has entered into its system-level testing program at NASA Goddard Space Flight Center (GSFC). In December 2013, ISIM successfully completed the first in a series of three cryo-vacuum tests, which included two flight science instruments. Since then, there have been full-fledged efforts towards the CV2 test scheduled to finish at the end of 2014. The complexity of the mission has generated challenging requirements that demand highly reliable system performance and capabilities from the Space Environment Simulator (SES) vacuum chamber. In order to satisfy the program requirements, GSFC had to develop unique structural and thermal hardware to test ISIM. Most noteworthy is a helium shroud structure and cooling system built in order to achieve operational temperatures below 20K (-253C). This paper: (1) provides an overview of the integrated mechanical and thermal facility systems required to achieve the objectives of JWST ISIM testing, (2) communicates the performance and challenges of the SES during the first ISIM test, and (3) summarizes the action plan to improve the system prior to the next test.

  4. Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv.'Tropic Sun').

    PubMed

    Colegate, Steven M; Gardner, Dale R; Joy, Robert J; Betz, Joseph M; Panter, Kip E

    2012-04-11

    Cultivation of Crotalaria juncea L. (Sunn Hemp cv. 'Tropic Sun') is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves, and seeds of 'Tropic Sun' were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems, and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid contents of the roots, stems, and leaves of immature plants were estimated to be 0.05, 0.12, and 0.01% w/w, respectively, whereas seeds were estimated to contain 0.15% w/w. PMID:22429238

  5. I-V and C-V studies of evaporated amorphous arsenic telluride film on crystalline silicon

    NASA Astrophysics Data System (ADS)

    Krupanidhi, S. B.; Srivastava, R. K.; Srinivas, K.; Bhattacharya, D. K.; Mansingh, Abhai

    1983-03-01

    The I-V characteristics of amorphous arsenic telluride (As2Te3) thin film on single-crystalline silicon substrates exhibit similarity with the characteristics of a heterojunction formed between crystalline semiconductors and the carrier transport, which involves more than one conduction mechanism at the interface. The recombination, tunnelling, and space-charge-limited carrier transport takes place at the interface successively, depending on the magnitude of the applied voltage. The heterojunction properties dominate at lower voltages (below 1 V), while at the higher voltages, the I-V characteristic is determined by the As2Te3 film. The temperature-dependent I-V characteristics confirm the formation of a heterojunction at the silicon-As2Te3 (film) interface. The built-in potential evaluated from the I-V characteristics (˜0.2 V) agrees well with the observed open-circuit photovoltage Voc. Contrary to the I-V characteristics, the C-V characteristics resemble the characteristics of a conventional metal-insulator-semiconductor (MIS) structure. This can be qualitatively explained by assuming the heterojunction to have a low breakdown potential (˜1.0 V), and the junction barrier capacitance in the breakdown region to be higher than either the film or the depletion capacitance.

  6. Some regulatory aspects of [(14)C]methylamine influx intoPisum sativum L. cv. Feltham First seedlings.

    PubMed

    Deane-Drummond, C E

    1986-03-01

    [(14)C]Methylamine influx intoPisum sativum L. cv. Feltham First seedlings showed Michaelis-Menten-type kinetics with apparentV max=49.2 ?mol·g(-1) FW·h(-1) and apparentK m=0.51 mM. The competitive interactions between ammonium and methylamine were most obvious when biphasic kinetics were assumed with saturation of the first phase at ?0.05 mM. The inhibitor constant for ammonium (K i)=0.027 mM. When [(14)C]methylamine was used in trace amounts with ammonium added as substrate, the influx of tracer showed Michaelis-Menten-type kinetics with apparentV max=3.46 ?mol·g(-1) FW·h(-1) and apparentK m=0.15 mM. The initial rate of net ammonium uptake corresponded with that found when [(14)C]methylamine was used to 'trace' ammonium influx. The latter was also stimulated by high pHo and inhibited by nitrate. Ammonium pretreatment±methionine sulphoximine or glutamine pretreatment of the seedlings inhibited subsequent [(14)C]methylamine influx, while methylamine or asparagine pretreatment stimulated [(14)C]methylamine influx. There was also a stimulatory effect of prior inoculation withRhizobium. The results are discussed in terms of current models for the regulation of ammonium uptake in plants. PMID:24232423

  7. Heavy metal accumulation and tolerance of energy grass (Elymus elongatus subsp. ponticus cv. Szarvasi-1) grown in hydroponic culture.

    PubMed

    Sipos, Gyula; Solti, Adám; Czech, Viktória; Vashegyi, Ildikó; Tóth, Brigitta; Cseh, Edit; Fodor, Ferenc

    2013-07-01

    Phytoremediation is a plant based, cost effective technology to detoxify or stabilise contaminated soils. Fast growing, high biomass, perennial plants may be used not only in phytoremediation but also in energy production. Szarvasi-1 energy grass (Elymus elongatus subsp. ponticus cv. Szarvasi-1), a good candidate for this combined application, was grown in nutrient solution in order to assess its Cd, Cu, Ni, Pb and Zn accumulation and tolerance. Its shoot metal accumulation showed the order Pb < Ni < Cu ? Cd < Zn. In parallel with this, Pb and Ni had no or very little influence on the growth, dry matter content, chlorophyll concentration and transpiration of the plants. Cu and Cd treatment resulted in significant decreases in all these parameters that can be attributed to Fe plaque formation in the roots suggested by markedly increased Fe and Cu accumulation. This came together with decreased shoot and root Mn concentrations in both treatments while shoot Cu and Zn concentrations decreased under Cd and Cu exposure, respectively. Zn treatment had no effect or even slightly stimulated the plants. This may be due to a slight stimulation of Fe translocation and a very efficient detoxification mechanism. Based on the average 300 mg kg?¹ (dry mass) Zn concentration which is 0.03% of the shoot dry mass the variety is suggested to be classified as Zn accumulator. PMID:23669138

  8. Herbicide Treatments Alter Carotenoid Profiles for 14C Tracer Production from Tomato (Solanum lycopersicum cv. VFNT cherry) Cell Cultures

    PubMed Central

    Engelmann, Nancy J.; Rogers, Randy B.; Lila, Mary Ann; Erdman, John W.

    2009-01-01

    Progress in learning about underlying carotenoid bioactivity mechanisms has been limited due to lack of commercially available radiolabeled lycopene (LYC), phytoene (PE), and phytofluene (PF). Tomato (Solanum lycopersicum cv. VFNT cherry) cell cultures have been treated to produce [14C]-PE and PF but with relatively low yields. To increase carotenoid production, two bleaching herbicides were administered during the culture incubation, 2-(4-chlorophenyl-thio) triethylamine and norflurazon separately or in combination to produce varying ratios of PE, PF, and LYC. Treatment with both herbicides resulted in optimal production of all three carotenoids. Subsequently, cultures were incubated in [14C] sucrose-containing media to produce labeled LYC, PE, and PF. Adding [14C] sucrose on day 1 of the 14-d culture incubation cycle to norflurazon-treated cultures led to a small increase in labeling efficiency compared to adding it on day 7. Improved culture conditions efficiently provided sufficient 14C-carotenoids for future cell culture and animal metabolic tracking studies. PMID:19489618

  9. Isolation and molecular characterization of an ethylene response factor NtERF1-1 in Nicotiana tabacum cv. Xanthi.

    PubMed

    Gao, Jun-Shan; Hu, Li; Xie, Peng; Meng, Yan; Cai, Yong-Ping; Lin, Yi

    2014-12-01

    Apetala2/Ethylene Response Factors (AP2/ERF) play important roles in regulating gene expression under abiotic and biotic stress in the plant kingdom. Here, we isolated a member of the AP2/ERF transcription factors, NtERF1-1, from Nicotiana tabcum cv. Xanthi NN carrying the N gene, which is resistant to Tobacco mosaic virus (TMV). NtERF1-1 encoded a putative protein of 229 amino acids with a predicted molecular mass of 24.58 kDa. Nucleotide sequence analysis showed that NtERF1-1 contained a conserved DNA-binding domain at the N-terminal. Comparison of amino acid sequences revealed that NtERF1-1 possessed high similarities to ERFs from diverse plants. Semi-quantitative and real-time quantitative RT-PCR analyses indicated that NtERF1-1 was up-regulated following TMV infection. In addition, we speculated that NtERF1-1 might participate in the signal transduction pathway of defence response inducted by the interaction between the N gene and TMV. PMID:25431417

  10. Accumulation of metals and its effects in Brassica juncea (L.) Czern. (cv. Rohini) grown on various amendments of tannery waste.

    PubMed

    Singh, Shraddha; Sinha, Sarita

    2005-09-01

    The metal accumulation potential and its tolerance in the plants of Brassica juncea (L.) Czern. (cv. Rohini) grown on various amendments of tannery sludge (10%, 25%, 35%, 50%, 75%, 100%) were studied after 30, 60, and 90 days after sowing. These plants were found to be effective accumulators of metals (Cr, Fe, Zn, and Mn); however, the seeds accumulated the least quantities of all the metals tested. The oil content of the seeds was found to increase up to 35% tannery sludge followed by a decrease. An increase in the photosynthetic pigments, protein, and sugar contents was recorded at the lower amendments of tannery sludge at initial exposure periods followed by a decrease compared to their respective controls. However, the malondialdehyde, proline, and ascorbic acid contents of the roots and leaves of the plant increased at all the sludge amendments and exposure periods, compared to their respective controls. The levels of cysteine and nonprotein thiol contents in the roots and leaves of the treated plant were found higher at all the sludge amendments and exposure periods except at 90 days, where a decrease was observed in the leaves at 100% tannery sludge as compared to their respective controls. The tolerance exhibited by the sludge-grown plants of B. juncea in the present study may be attributed to the enhanced level of the antioxidants induced under stress conditions. PMID:15978297

  11. A peroxisomally localized acyl-activating enzyme is required for volatile benzenoid formation in a Petuniaxhybrida cv. 'Mitchell Diploid' flower.

    PubMed

    Colquhoun, Thomas A; Marciniak, Danielle M; Wedde, Ashlyn E; Kim, Joo Young; Schwieterman, Michael L; Levin, Laura A; Van Moerkercke, Alex; Schuurink, Robert C; Clark, David G

    2012-08-01

    Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petunia×hybrida cv. 'Mitchell Diploid' (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway intermediate by a coenzyme A (CoA) dependent, ?-oxidative scheme. Metabolic flux analysis, reverse genetics, and biochemical characterizations of key enzymes in this pathway have supported this putative concept. However, the theoretical first enzymatic reaction, which leads to the production of cinnamoyl-CoA, has only been physically demonstrated in a select number of bacteria like Streptomyces maritimus through mutagenesis and recombinant protein production. A transcript has been cloned and characterized from MD flowers that shares high homology with an Arabidopsis thaliana transcript ACYL-ACTIVATING ENZYME11 (AtAAE11) and the S. maritimus ACYL-COA:LIGASE (SmEncH). In MD, the PhAAE transcript accumulates in a very similar manner as bona fide FVBP network genes, i.e. high levels in an open flower petal and ethylene regulated. In planta, PhAAE is localized to the peroxisome. Upon reduction of PhAAE transcript through a stable RNAi approach, transgenic flowers emitted a reduced level of all benzenoid volatile compounds. Together, the data suggest that PhAAE may be responsible for the activation of t-cinnamic acid, which would be required for floral volatile benzenoid production in MD. PMID:22771854

  12. 5S program to reduce change-over time on forming department (case study on CV Piranti Works temanggung)

    NASA Astrophysics Data System (ADS)

    Rosiana Dewi, Septika; Setiawan, Budi; P, Susatyo Nugroho W.

    2013-06-01

    Productivity is one aspect that determines the success of a company in the competitive world of business. There are seven main types of activities that do not have value-added in manufacturing processes such as overproduction, waiting time, transportation, excess inventory, unnecessary motion and defects. The whole activity is a waste (waste) that can cause harm to the Company. Therefore, in production activities is important to pay attention so that the objectives of production productivity can be achieved. Problems experienced by CV Piranti Works is a production target is not achieved resulting in a lost sale raises the cost of which can cause harm to the Company. From the analysis conducted major known cause of the problem is the length of time required for changeover. This is supported by the high non-value added activity in the changeover activities. Lean Manufacturing is an approach to make system more efficient by reducing waste. This study refers to the book compiled by Takashi Osada (2004) and several other references. In this research used method 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) for the of forming departement. The purpose of this research is to design a work environment using the 5S method (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) and make arrangement of equipment and working tool cabinet design with TRIZ methods. From these results, is expected to eliminate or reduce of non-value added activity and improved the changeover time so as to meet production targets completion of the company.

  13. Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3-year comparison.

    PubMed

    Spitaler, Renate; Winkler, Andrea; Lins, Isabella; Yanar, Sema; Stuppner, Hermann; Zidorn, Christian

    2008-03-01

    In continuation of our studies of altitudinal effects on secondary metabolite profile of flowering heads from taxa of the Asteraceae, we investigated phenolic contents and radical scavenging potential from cultivated plants of Arnica montana cv. ARBO during the growing seasons 2003, 2004, and 2005. By conducting experiments on potted plants, we excluded that differences in phenolic contents from plants grown at different altitudes were related primarily to differences in soil composition at these sites. To assess altitudinal and interseasonal variation, plants of A. montana cultivar ARBO were grown in nine experimental plots at altitudes between 590 and 2,230 m at Mount Patscherkofel near Innsbruck, Austria. In all growing seasons and regardless of the soil the plants were grown in, the proportion of flavonoids with vicinal-free hydroxy groups in ring B to flavonoids lacking this feature, and the total amount of caffeic acid derivatives, significantly increased with elevation. These increases of antioxidant phenolics corresponded to an increase of the radical scavenging potential of extracts from plants grown at different altitudes. The results are discussed in regard to previous studies that suggest that enhanced UV-B radiation and decreased temperatures trigger augmented biosynthesis of UV-absorbing and antioxidant phenolics in higher plants. PMID:18259818

  14. Some observations of the effects of applied magnetic fields on growth of Avena coleoptiles. [Avena sativa cv. Garry

    SciTech Connect

    Brown, D.S.; Gretz, M.; Brown, R.M. Jr.

    1986-04-01

    Applied magnetic fields (5000-18,000 Gauss) disrupt the pattern of cellulose microfibril deposition in cells of Avena sativa cv. Garry coleoptiles. Freeze fracture reveals the microfibrils of the innermost wall layers to be randomly oriented, rather than parallel and transverse to the elongation axis as seen in control plants. Whole plants exposed to the magnetic field at any field intensity tested (5000-18,000 Gauss) displayed inhibited elongation growth. After two hours in a 5000 G field, plants show some recovery of elongation growth while maintained in the field, with elongation rates of 0.2 + 0.06 mm/hr as compared to control plants with 0.9 + 0.12 mm/hr elongation. Avena seeds which were germinated directly between pole pieces of a 5000 Gauss magnet produced slow-growing seedlings whose roots and coleoptiles grew toward opposite magnetic poles. Audus (1960) coined the phrase magnetotropism to describe a similar phenomenon in pollen tubes growing in magnetic fields.

  15. The use of Lactobacillus pentosus 1MO to shorten the debittering process time of black table olives (Cv. Itrana and Leccino): a pilot-scale application.

    PubMed

    Servili, Maurizio; Settanni, Luca; Veneziani, Gianluca; Esposto, Sonia; Massitti, Ombretta; Taticchi, Agnese; Urbani, Stefania; Montedoro, Gian Francesco; Corsetti, Aldo

    2006-05-31

    Fifty lactobacilli isolated from black table olive brines were evaluated for their salt tolerance, resistance to oleuropein and verbascoside, and ability to grow in modified filter-sterilized brines. A strain of Lactobacillus pentosus was selected and used as a starter to ferment, in pilot plant, black olives (Itrana and Leccino cv.) in brines modified for pH, carbohydrate, and growth factor concentrations, at 28 degrees C. The temperature-controlled fermentation of Leccino cv. olives resulted in obtaining ready-to-eat, high-quality table olives in a reduced-time process. HPLC analysis of phenolic compounds from fermented olives showed a decrease of oleuropein, a glucoside secoiridoid responsible for the bitter taste of olive drupes, and an increase of the hydroxytyrosol concentration. The selected strain of L. pentosus (1MO) allowed the reduction of the debittering phase period to 8 days. PMID:16719508

  16. Gas-exchange activity, carbohydrate status, and protein turnover in root nodule subpopulations of field pea ( Pisum sativum L. cv. Century)

    Microsoft Academic Search

    Per-Åke Vikman; J. Kevin Vessey

    1993-01-01

    Root nodule ontogeny was followed in different parts of the root system of field peas (Pisum sativum L. cv. Century) to investigate the contribution to total nitrogen fixation by different nodule subpopulations. Seed-inoculated\\u000a plants were grown to maturity in controlled-environment growth chambers. In a flow-through system nitrogenase activity (H2-evolution in air) and nodulated-root respiration (net CO2-evolution) were measured weekly or

  17. Alteration of mitochondrial genomes containing atpA genes in the sexual progeny of cybrids between Raphanus sativus cms line and Brassica napus cv. Westar

    Microsoft Academic Search

    Takako Sakai; Jun Imamura

    1992-01-01

    We have investigated the fate of the mitochondrial genomes of cybrids derived from “donor-recipient” protoplast fusion between X-irradiated Raphanus sativus (cms line) and iodoacetamide-treated Brassica napus cv. Westar. Two out of ten fusion products were male-sterile with the diploid chromosome number of B. napus. The mitochondrial (mt) genomes of the cybrids and their progeny were further analyzed by DNA-DNA hybridizaion

  18. Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree)

    Microsoft Academic Search

    Gert Ooms; David Twell; Margreet E. Bossen; J. Harry C. Hoge; Michael M. Burrell

    1986-01-01

    Expression of TL-DNA from Agrobacterium rhizogenes plasmid pRi 1855 was examined in a transformed derivative of Solanum tuberosum cv. Desiree, D9X8a. Northern blot analysis identified at least nine TL-DNA coded transcripts in roots, shoots and tubers but their relative abundance differed within and between organs. This revealed a distinctive pattern of organ specified differential expression. Grafting experiments showed that the

  19. Does nitrogen supply affect the response of wheat (Triticum aestivum cv. Hanno) to the combination of elevated CO2 and O3?

    Microsoft Academic Search

    Joao Cardoso-Vilhena; Jeremy Barnes

    2001-01-01

    Spring wheat (Triticum aestivum cv. Hanno) was grown at ambient (350 mmol mol? 1) or elevated CO2 (700 mmol mol? 1) in charcoaluPurafil1-filtered air (CFA - 5n mol mol ? 1) or ozone (CFA q75 nmol mol? 1 7h d? 1) at three levels of N supply (1.5, 4 and 14 mM NO?3 ), to test the hypothesis that the

  20. Zygomatic Smile Detection: The Semi-Supervised Haar Training of a Fast and Frugal System: A Gift to OpenCV Community

    Microsoft Academic Search

    D. D. Hromada; C. Tijus; S. Poitrenaud; J. Nadel

    2010-01-01

    Five different OpenCV-compatible XML haar cascades of zygomatic smile detectors as well as five SMILEsamples from which these detectors were derived had been trained and are presented hereby as a new open source SMILEsmileD package. Samples have been extended in an incremental learning fashion, exploiting previously trained detector in order to add and label new elements of positive example set.

  1. Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV1 cells

    Microsoft Academic Search

    Francesca Navone; Joshua Niclas; Nora Hom-Booher; Lynne Sparks; Harris D. Bernstein; Gretchen McCaffrey; Ronald D. Vale

    1992-01-01

    To understand the interactions between the microtubule-based motor protein kinesin and intracel- lular components, we have expressed the kinesin heavy chain and its different domains in CV-1 monkey kidney epithelial cells and examined their distributions by im- munofluorescence microscopy. For this study, we cloned and sequenced cDNAs encoding a kinesin heavy chain from a human placental library. The hu- man

  2. Suitability of mycorrhiza-defective mutant\\/wildtype plant pairs ( Solanum lycopersicum L. cv Micro-Tom) to address questions in mycorrhizal soil ecology

    Microsoft Academic Search

    Matthias C. Rillig; Philip W. Ramsey; James E. Gannon; Daniel L. Mummey; Vijay Gadkar; Yoram Kapulnik

    2008-01-01

    Despite the importance of arbuscular mycorrhizal fungi (AMF) to ecosystem processes, few experimental tools are available\\u000a to quantify AMF contributions to process rates. In this study we examine the efficacy of an experimental system consisting\\u000a of wildtype (WT) and different non-mycorrhizal (Myc?) genotype pairs of tomato (Solanum lycopersicum L.), specifically focusing on cv Micro-Tom. Two conditions necessary to make such

  3. Application of silver nanoparticles contained in ethanol as a working fluid in an oscillating heat pipe with a check valve (CLOHP/CV): a thermodynamic behaviour study

    NASA Astrophysics Data System (ADS)

    Bhuwakietkumjohn, N.; Parametthanuwat, T.

    2015-01-01

    This research focused on the thermal behaviour of oscillating heat pipe with a check valve (CLOHP/CV). The CLOHP/CV was made from a copper capillary tube with an internal diameter of 2.03 mm, 40 turns, and 2 check valves. The CLOHP/CV had three sections: the evaporator and adiabatic condenser of a length of 50, 100, and 150 mm. The angles of inclinations were 90°, 80°, 60°, 40°, 20° and 0° at normal operating conditions. The two working fluids were an ethanol and silver nanoparticles concentration of 0.5 wt% contained in the ethanol (NE). The filling ratio was 50 % with respect to the total volume of the tube. The operating temperature (T w ) was 45, 65, 85, 105, 125 and 150 °C. The results of the study showed that the heat flux increased significantly when the operating temperature increased, and the heat flux increased when the L e decreased from 150 to 50 mm. Furthermore, the thermal behaviour of CLOHP/CV showed the highest heat flux of 2,012.63 W/m2 at L e of 50, with an angle of inclination of 90° and operating temperature of 150 °C with NE. The behaviour of 0.5 wt% of silver nanoparticles contained in the ethanol produced a good contact angle. The 0.5 wt% of silver nanoparticles decreased the wet ability, thus increasing the thermal behaviour. The optimum concentration for the addition of silver nanoparticles in the working fluid was 0.5 wt%.

  4. The lipid composition, fluidity, and Mg 2+ ATPase activity of rice ( Oryza sativa L. cv. Bahia) shoot plasma membranes: effects of ABA and GA 3

    Microsoft Academic Search

    C. Martínez-Cortina; R. Ros; D. T. Cooke; C. S. James; A. Sanz

    1992-01-01

    Six-day-old rice plants (Oryza sativa L., cv Bahia) were grown for 5 days more in nutrient solution culture containing 10?5 M abscisic acid (ABA) or gibberellic acid (GA3) (treated plants). Plasma membrane (PM) vesicles were isolated from the shoots of treated or control plants, and ATPase hydrolytic\\u000a and proton-pumping activity, fluidity, and free sterol and phospholipid composition were determined. Both

  5. Intake and digestion by lambs of dwarf elephant grass ( Pennisetum purpureum Schum. cv. Mott) hay or hay supplemented with urea and different levels of cracked corn grain

    Microsoft Academic Search

    G. V. Kozloski; L. M. Bonnecarrère Sanchez; R CADORINJR; M. V. Reffatti; D. Perez Neto; L. D. Lima

    2006-01-01

    This study was carried out to evaluate intake, digestibility, ruminal fermentation, nitrogen (N) retention and ruminal microbial protein synthesis in lambs fed dwarf elephant grass (Pennisetum purpureum Schum. cv. Mott) hay or hay supplemented with urea and 0, 5, 10 or 15g\\/kg of live weight (LW) of cracked corn grain. Ten lambs (mean LW of 28±0.9kg), housed in metabolic cages,

  6. Long-Term Fungal Inhibitory Activity of Water-Soluble Extracts of Phaseolus vulgaris cv. Pinto and Sourdough Lactic Acid Bacteria during Bread Storage

    Microsoft Academic Search

    Rossana Coda; Carlo G. Rizzello; Franco Nigro; Maria De Angelis; Philip Arnault; Marco Gobbetti

    2008-01-01

    The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts

  7. Production of plants resistant to Alternaria carthami via organogenesis and somatic embryogenesis of safflower cv. NARI-6 treated with fungal culture filtrates

    Microsoft Academic Search

    J. Vijaya Kumar; B. D. Ranjitha Kumari; G. Sujatha; Enrique Castaño

    2008-01-01

    The present study describes a system for efficient plant regeneration via organogenesis and somatic embryogenesis of safflower\\u000a (Carthamus\\u000a tinctorius L.) cv. NARI-6 in fungal culture filtrates (FCF)-treated cultures. FCF was prepared by culturing Alternaria carthami fungal mycelia in selection medium for host-specific toxin production. Cotyledon explants cultured on callus induction medium\\u000a with different levels of FCF (10–50%) produced embryogenic callus.

  8. Construction and characterization of a plant transformation-competent BIBAC library of the black Sigatoka-resistant banana Musa acuminata cv. Tuu Gia (AA)

    Microsoft Academic Search

    E. Ortiz-Vázquez; D. Kaemmer; H. B. Zhang; J. Muth; M. Rodríguez-Mendiola; C. Arias-Castro; Andrew James

    2005-01-01

    A plant transformation-competent binary bacterial artificial chromosome (BIBAC) library was constructed from Musa acuminata cv. Tuu Gia (AA), a black Sigatoka-resistant diploid banana. After digestion of high-molecular-weight banana DNA by HindIII, several methods of DNA size selection were tested, followed by ligation, using a vector\\/insert molar ratio of 4:1. The library consists of 30,700 clones stored in 80 384-well microtiter

  9. Physical analysis of the complex rye ( Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco

    Microsoft Academic Search

    B.-J. Shi; J. P. Gustafson; J. Button; J. Miyazaki; M. Pallotta; N. Gustafson; H. Zhou; P. Langridge; N. C. Collins

    2009-01-01

    Rye is a diploid crop species with many outstanding qualities, and is important as a source of new traits for wheat and triticale\\u000a improvement. Rye is highly tolerant of aluminum (Al) toxicity, and possesses a complex structure at the Alt4 Al tolerance locus not found at the corresponding locus in wheat. Here we describe a BAC library of rye cv.

  10. Formation and development of embryo and endosperm in intra- and inter-specific crosses of Asparagus officinalis and A. densiflorus cv. Sprengeri

    Microsoft Academic Search

    O. N Marcellán; E. L Camadro

    1999-01-01

    Garden asparagus, Asparagus officinalis L. (2n=2x=20) is reproductively isolated from the related ornamental species Asparagus densiflorus (Kunth) Jessop cv. Sprengeri (2n=6x=60) by incompatibility barriers at the pollen–stigma and pollen–style levels, plus stronger post-stylar barriers, which have not been described, in pollen–pistil-compatible genotypes. To study the latter, intra- and inter-specific crosses were made using 25 plants of A. officinalis and 29

  11. Cryopreservation of in vitro-cultured multiple bud clusters of asparagus ( Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification

    Microsoft Academic Search

    H. Kohmura; A. Sakai; S. Chokyu; T. Yakuwa

    1992-01-01

    A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated “bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis L.cv. Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to -196°C.

  12. HRTEM and EFTEM Observations of Matrix in the Oxidized CV3 Chondrite ALH 84028: Implications for the Origins of Matrix Olivines

    NASA Technical Reports Server (NTRS)

    Abreu, Neyda M.; Brearley, Adrian J.

    2003-01-01

    The determination of the nature, distribution, and origin of organic material in carbonaceous chondrites is fundamental to understanding early solar nebular conditions and the origin of life. Using a variety of extraction techniques, followed by detailed chemical analysis, an extensive suite of organic compounds has been identified in carbonaceous chondrites. These data have provided key information on the diversity and isotopic composition of the organic component in chondrites. However, one disadvantage of extraction techniques is that all information regarding the spatial distribution of the organics on a fine scale is lost. This is especially important for the insoluble macromolecular carbon, which constitutes approximately 70% of the carbon in carbonaceous chondrites such as Murchison. The distribution and mineralogical associations may provide important constraints on the possible origins of the carbonaceous material. Our previous studies of the CV3 chondrites Allende and Vigarano have demonstrated that energy filtered transmission electron microscopy (EFTEM), combined with high resolution TEM (HRTEM) are powerful tools for the in situ characterization of insoluble organic matter in carbonaceous chondrites. In this study, we have used SEM and TEM techniques to characterize the matrix mineralogy of the CV3 chondrite ALH 84028 and examine the distribution and mineralogical associations of carbon. We are especially interested in establishing whether the occurrence of poorly graphitized carbon (PGC), observed in Allende matrix olivines, is common to all oxidized CV3 chondrites or is a unique feature of Allende.

  13. Hydrothermal origin of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A CAI from the Northwest Africa 2086 CV3 chondrite

    NASA Astrophysics Data System (ADS)

    Fintor, Krisztian; Park, Changkun; Nagy, Szabolcs; Pál-Molnár, Elemér; Krot, Alexander N.

    2014-05-01

    We report an occurrence of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A calcium-aluminum-rich inclusion (CAI) from the CV3 (Vigarano-like) carbonaceous chondrite Northwest Africa 2086. Dmisteinbergite occurs as approximately 10 ?m long and few micrometer-thick lath-shaped crystal aggregates in altered parts of the CAI, and is associated with secondary nepheline, sodalite, Ti-poor Al-diopside, grossular, and Fe-rich spinel. Spinel is the only primary CAI mineral that retained its original O-isotope composition (?17O ~ -24‰); ?17O values of melilite, perovskite, and Al,Ti-diopside range from -3 to -11‰, suggesting postcrystallization isotope exchange. Dmisteinbergite, anorthite, Ti-poor Al-diopside, and ferroan olivine have 16O-poor compositions (?17O ~ -3‰). We infer that dmisteinbergite, together with the other secondary minerals, formed by replacement of melilite as a result of fluid-assisted thermal metamorphism experienced by the CV chondrite parent asteroid. Based on the textural appearance of dmisteinbergite in NWA 2086 and petrographic observations of altered CAIs from the Allende meteorite, we suggest that dmisteinbergite is a common secondary mineral in CAIs from the oxidized Allende-like CV3 chondrites that has been previously misidentified as a secondary anorthite.

  14. The effects of tropospheric ozone and elevated carbon dioxide on potato (Solanum tuberosum L. cv. Bintje) growth and yield.

    PubMed

    Persson, K; Danielsson, H; Selldén, G; Pleijel, H

    2003-07-01

    An open-top chamber experiment with field grown potato (Solanum tuberosum L. cv. Bintje) was performed in the south-west of Sweden in 1998. The experiment was aimed to investigate individual and interactive impacts of elevated ozone (O(3)) and carbon dioxide (CO(2)) on potato growth and yield. Treatments were ambient and elevated CO(2) with or without the addition of 20 nmol mol(-1) O(3). In addition, plants were grown in ambient air plots. Plants grown in elevated CO(2) and O(3) had a reduced tuber yield, compared to control, by 2% and 8%, respectively. These effects were, however, not statistically significant. Elevated CO(2) caused a significantly larger number of tubers of lower average size, while elevated O(3) induced significantly lower tuber dry matter content. The number of tuber initials (tubers <15 mm) found at harvest was significantly lower in elevated O(3). Visible O(3) leaf injury appeared earlier and the number of yellow leaves 89 days after emergence was significantly larger in elevated O(3). In elevated CO(2), haulm dry weight was significantly (15%) lower at harvest and the haulm/tuber ratio was significantly reduced. There were significant interactive effects of O(3) and CO(2) on the final number of leaves and on stem height. Plants in elevated CO(2) reached final stem height earlier than in the other treatments. In summary, it was concluded: (a) that anticipated future O(3) levels are likely to have a negative impact on potato growth, although tuber yield effects may be hard to demonstrate experimentally in this plant due to large variation; (b) that whether any possible beneficial effect of elevated CO(2) on the magnitude of tuber yield will be expressed, depend on the climatic conditions prevailing; and (c) that potato growth responses to CO(2) and O(3) are not simply additive. PMID:12812743

  15. Proteins Involved in Distinct Phases of Cold Hardening Process in Frost Resistant Winter Barley (Hordeum vulgare L.) cv Luxor

    PubMed Central

    Hlavá?ková, Iva; Vítámvás, Pavel; Šantr??ek, Ji?í; Kosová, Klára; Zelenková, Sylva; Prášil, Ilja Tom; Ovesná, Jaroslava; Hynek, Radovan; Kodí?ek, Milan

    2013-01-01

    Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in relation to short and long term periods of cold followed by a brief frost treatment were studied in order to disclose proteins responsible for the cold hardening process in distinct plant tissues. The mentioned changes have been monitored using two dimensional difference gel electrophoresis (2D-DIGE) with subsequent peptide-mapping protein identification. Regarding approximately 600–700 distinct protein spots detected on 2D gels, there has been found at least a two-fold change after exposure to low temperatures in about 10% of proteins in leaves and 13% of proteins in crowns. Protein and nitrogen metabolic processes have been influenced by low temperature to a similar extent in both tissues while catabolism, carbohydrate metabolism and proteins involved in stress response have been more affected in crowns than in leaves. The range of changes in protein abundance was generally higher in leaves and chloroplast proteins were frequently affected which suggests a priority to protect photosynthetic apparatus. Overall, our data proved existence of slightly different response strategies to low temperature stress in crowns and leaves, i.e., tissues with different biological role. Moreover, there have been found several proteins with large increase in accumulation, e.g., 33 kDa oxygen evolving protein of photosystem II in leaves and “enhanced disease susceptibility 1” in crowns; these proteins might have potential to indicate an enhanced level of frost tolerance in barley. PMID:23584021

  16. Root exudation and root development of lettuce (Lactuca sativa L. cv. Tizian) as affected by different soils

    PubMed Central

    Neumann, G.; Bott, S.; Ohler, M. A.; Mock, H.-P.; Lippmann, R.; Grosch, R.; Smalla, K.

    2014-01-01

    Development and activity of plant roots exhibit high adaptive variability. Although it is well-documented, that physicochemical soil properties can strongly influence root morphology and root exudation, particularly under field conditions, a comparative assessment is complicated by the impact of additional factors, such as climate and cropping history. To overcome these limitations, in this study, field soils originating from an unique experimental plot system with three different soil types, which were stored at the same field site for 10 years and exposed to the same agricultural management practice, were used for an investigation on effects of soil type on root development and root exudation. Lettuce (Lactuca sativa L. cv. Tizian) was grown as a model plant under controlled environmental conditions in a minirhizotrone system equipped with root observation windows (rhizoboxes). Root exudates were collected by placing sorption filters onto the root surface followed by subsequent extraction and GC-MS profiling of the trapped compounds. Surprisingly, even in absence of external stress factors with known impact on root exudation, such as pH extremes, water and nutrient limitations/toxicities or soil structure effects (use of sieved soils), root growth characteristics (root length, fine root development) as well as profiles of root exudates were strongly influenced by the soil type used for plant cultivation. The results coincided well with differences in rhizosphere bacterial communities, detected in field-grown lettuce plants cultivated on the same soils (Schreiter et al., this issue). The findings suggest that the observed differences may be the result of plant interactions with the soil-specific microbiomes. PMID:24478764

  17. Calcium isotopic ratios and rare earth element abundances in refractory inclusions from the Allende CV3 chondrite

    NASA Astrophysics Data System (ADS)

    Huang, Shichun; Farkaš, Juraj; Yu, Gang; Petaev, Michail I.; Jacobsen, Stein B.

    2012-01-01

    Refractory inclusions in primitive meteorites are the oldest objects formed in the Solar System. They exhibit large mass-dependent isotopic effects in many elements, including Si, Mg and Ca, indicative of their complex origin. We report data for both mass-dependent (?) and mass-independent (?) Ca isotopic effects, and rare earth element (REE) abundances in six refractory inclusions from the Allende CV3 meteorite. Our data reveal large mass-dependent Ca isotopic effects in these refractory inclusions, with ?44/40Ca ranging from -5.60 to +0.35 (relative to NIST SRM 915a), which are significantly lower than that of the bulk silicate Earth (?44/40Ca = 1.05 ± 0.04). Importantly, ?44/40Ca is correlated with REE patterns: refractory inclusions with Group II REE patterns have lighter Ca isotope compositions compared to those with Group I REE patterns. Specifically, the studied refractory inclusions form negative 44Ca/40Ca-Tm/Er and 44Ca/40Ca-Nd/Er trends, implying segregation of up to 3% of an ultrarefractory evaporation residue from a chondritic reservoir prior to the formation of refractory inclusions. This residue would have to be characterized by high 44Ca/40Ca and low Tm/Er and Nd/Er. At 2? levels of ±0.5 and ±1.5 for ?40/44Ca and ?43/44Ca, respectively, the six refractory inclusions studied show no detectable 40Ca or 43Ca anomalies relative to the terrestrial standards. However, five out of the six refractory inclusions do show a 48Ca excess of several ?-units, clearly resolvable from the terrestrial standards.

  18. Prediction of evapotranspiration and grain yield of rice ( Oryza sativa L. cv Thriveni) in a humid tropical climate

    NASA Astrophysics Data System (ADS)

    Rao, A. S.; Alexander, D.

    1988-06-01

    In a humid tropical climate at Pattambi (10° 48' N, 76° 12' E), the evapotranspiration (ET) rates of rice ( Oryza sativa L. cv. Thriveni) were 2.8 5.7 mm/day during the first crop season (May September) and 6.2 9.1 mm/day during the second crop season (September January). The crop was grown at the Station in the irrigated lowlands on sandy soils with average yields of 3025 and 2925 kg/ha in the first and second cropping seasons, respectively. The seasonal ET, water requirements, water use efficiency and field water use efficiency of the crop were 400 mm, 1150 mm, 7.56 and 2.63 kg/ha per mm in the first crop season and 650 mm, 1500 mm, 4.50 and 1.95 kg/ha per millimetre in the second crop season. Using the reference crop ET computed by Blaney-Criddle, Radiation, and Penman methods and measured evaporation from class A pan, Colorado and GGI 3000 pans, the crop coefficients were worked out. Correlations between weather parameters and the biomass of rice were obtained. The grain yield (Y, in kg/ha) of the crop was predicted using the equation Y=1.71 Y 0-56 S+85 F-2430 ( N=8, r=0.920), where Y 0 is the sample of biomass of the rice at flowering in kg/ha, S and F are the duration of sunshine hours and maximum air temperatures (°C) between the 46th day of transplanting and maturity.

  19. Antioxidant activity and chemical components as potential anticancer agents in the olive leaf (Olea europaea L. cv Leccino.) decoction.

    PubMed

    De Marino, Simona; Festa, Carmen; Zollo, Franco; Nini, Antonella; Antenucci, Lina; Raimo, Gennaro; Iorizzi, Maria

    2014-01-01

    Epidemiological studies have shown that a reduced risk of chronic diseases such as cancer and cardiovascular diseases is correlated with a regular consumption of fruits and vegetable, many of which are rich in polyphenols. The additive and synergistic effect of phytochemicals in fruits and vegetables may reduce chronic diseases related to oxidative stress in human body. Olea europaea L. leaf are rich in phenolic components, which have been proposed to play a role in cancer prevention. The purpose of this study was to identify the main components in the Olea europaea L. leaf (cv. Leccino) preserved during the decoction preparation, in order to delineate the antioxidant activities of the crude extracts and its isolated compounds by using different in vitro assays including DPPH radicalscavenging capacity, total antioxidant capacity (TAC), xanthine oxidase (XO) inhibitory effect and the ability to delay the linoleic acid peroxidation process (ALP). The aqueous decoction was partitioned obtaining four extracts and the n-butanol extract showed the highest antioxidant activity and the highest total phenolic content. Phytochemical investigation leads to the isolation of thirteen secondary metabolites including simple phenolics, flavonoids, secoiridoids whose structures were elucidated by spectroscopic data (1D and 2D NMR) and spectrometric techniques. A significant free radical scavenging effect against DPPH has been evidenced in fraxamoside (1) (EC50 62.6 µM) and taxifolin (5) (EC50 50.0 µM), isolated for the first time from the water decoction. The most active compound in the TAC evaluation, was the 3,4 dihydro-phenyl glycol (8) (0.90 caffeic acid equiv.) while taxifolin and fraxamoside resulted as the most efficient inhibitors of XO activity (IC50 2.7 and 5.2 µM, respectively). Secoxyloganin (4), oleuropein (2) and tyrosol (6) showed the highest ALP activity. This study adds to the growing body of data supporting the bioactivities of phytochemicals and their potential impact on human health. PMID:25102361

  20. A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata

    PubMed Central

    Camps, Céline; Kappel, Christian; Lecomte, Pascal; Léon, Céline; Gomès, Eric; Coutos-Thévenot, Pierre; Delrot, Serge

    2010-01-01

    Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays comprising 14?500 probes were used to compare, under three experimental conditions (in vitro, in the greenhouse, and in the vineyard), foliar material of infected symptomatic plants (S+R+), infected asymptomatic plants (S–R+), and healthy plants (S–R–). These plants were characterized by symptom notation after natural (vineyard) or experimental (in vitro and greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative real-time PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (Erysiphe necator, Plasmopara viticola, and Botrytis cinerea). (i) Five functional categories of genes, that is those involved in metabolism, defence reactions, interaction with the environment, transport, and transcription, were up-regulated in S+R+ plants compared with S–R– plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also up-regulated after infection by powdery mildew, downy mildew, and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and the polypeptide fraction secreted by Eutypa. PMID:20190040

  1. Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)

    SciTech Connect

    Lynch, D.V.; Steponkus, P.L. (Cornell Univ., Ithaca, NY (USA))

    1987-01-01

    Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the plasma membrane, accounting for 16 mole % of the total lipid. Phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine with lesser amounts of phosphatidylglycerol, phosphatidic acid, phosphatidylserine, and phosphatidylinositol, comprised only 32 mole% of the total lipid in NA samples. Following cold acclimation, free sterols increased from 33 to 44 mole %, while steryl glucosides and acylated steryl glucosides decreased from 15 to 6 mole % and 4 to 1 mole %, respectively. Sterol analyses of these lipid classes demonstrated that free {beta}-sitosterol increased from 21 to 32 mole % (accounting for the increase in free sterols as a class) at the expense of sterol derivatives containing {beta}-sitosterol. Glucocerebrosides decreased from 16 to 7 mole % of the total lipid following cold acclimation. In addition, the relative proportions of associated hydroxy fatty acids, including 22:0 (h), 24:0 (h), 22:1 (h), and 24:1 (h) were altered. The phospholipid content of the plasma membrane fraction increased to 42 mole % of the total lipid following cold acclimation. Although the relative proportions of the individual phospholipids did not change appreciably after cold acclimation, there were substantial differences in the molecular species. Di-unsaturated molecular species of phosphatidylcholine and phosphatidylethanolamine increased following acclimation. These results demonstrate that cold acclimation results in substantial changes in the lipid composition of the plasma membrane.

  2. PhDAHP1 is required for floral volatile benzenoid/phenylpropanoid biosynthesis in Petunia × hybrida cv 'Mitchell Diploid'.

    PubMed

    Langer, Kelly M; Jones, Correy R; Jaworski, Elizabeth A; Rushing, Gabrielle V; Kim, Joo Young; Clark, David G; Colquhoun, Thomas A

    2014-07-01

    Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis consists of numerous enzymatic and regulatory processes. The initial enzymatic step bridging primary metabolism to secondary metabolism is the condensation of phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) carried out via 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE (DAHP) synthase. Here, identified, cloned, localized, and functionally characterized were two DAHP synthases from the model plant species Petunia × hybrida cv 'Mitchell Diploid' (MD). Full-length transcript sequences for PhDAHP1 and PhDAHP2 were identified and cloned using cDNA SMART libraries constructed from pooled MD corolla and leaf total RNA. Predicted amino acid sequence of PhDAHP1 and PhDAHP2 proteins were 76% and 80% identical to AtDAHP1 and AtDAHP2 from Arabidopsis, respectively. PhDAHP1 transcript accumulated to relatively highest levels in petal limb and tube tissues, while PhDAHP2 accumulated to highest levels in leaf and stem tissues. Through floral development, PhDAHP1 transcript accumulated to highest levels during open flower stages, and PhDAHP2 transcript remained constitutive throughout. Radiolabeled PhDAHP1 and PhDAHP2 proteins localized to plastids, however, PhDAHP2 localization appeared less efficient. PhDAHP1 RNAi knockdown petunia lines were reduced in total FVBP emission compared to MD, while PhDAHP2 RNAi lines emitted 'wildtype' FVBP levels. These results demonstrate that PhDAHP1 is the principal DAHP synthase protein responsible for the coupling of metabolites from primary metabolism to secondary metabolism, and the ultimate biosynthesis of FVBPs in the MD flower. PMID:24815009

  3. Transformation of carotenoid biosynthetic genes using a micro-cross section method in kiwifruit (Actinidia deliciosa cv. Hayward).

    PubMed

    Kim, Misun; Kim, Seong-Cheol; Song, Kwan Jeong; Kim, Ho Bang; Kim, In-Jung; Song, Eun-Young; Chun, Seung-Jong

    2010-12-01

    Genetic transformation using a micro-cross section (MCS) technique was conducted to improve the carotenoid content in kiwifruit (Actinidia deliciosa cv. Hayward). The introduced carotenoid biosynthetic genes include geranylgeranyl diphosphate synthase (GGPS), phytoene desaturase (PDS), ?-carotene desaturase (ZDS), ?-carotene hydroxylase (CHX), and phytoene synthase (PSY). The transformed explants were selected on half-strength MS medium containing 0.001 mg l(-1) of 2,4-D and 0.1 mg l(-1) of zeatin, either 5 mg l(-1) hygromycin or 25 mg l(-1) kanamycin, and 500 mg l(-1) cefotaxime. The genomic PCR, genomic Southern blot analysis, and RT-PCR were performed to confirm the integration and expression of the transgenes. The transformation efficiencies of either kanamycin- or hygromycin-resistant shoots ranged from 2.9 to 22.1% depending on the target genes, and from 2.9 to 24.2% depending on the reporter genes. The selection efficiencies ranged from 66.7 to 100% for the target genes and from 95.8 to 100% for the reporter genes. Changes of carotenoid content in the several PCR-positive plants were determined by UPLC analysis. As a result, transgenic plants expressing either GGPS or PSY increased about 1.2- to 1.3-fold in lutein or ?-carotene content compared to non-transgenic plants. Our results suggest that the Agrobacterium-mediated transformation efficiency of kiwifruit can be greatly increased by this MCS method and that the carotenoid biosynthetic pathway can be modified in kiwifruit by genetic transformation. Our results further suggest that GGPS and PSY genes could be major target genes to increase carotenoid contents in kiwifruit. PMID:20842364

  4. A Lectin with Highly Potent Inhibitory Activity toward Breast Cancer Cells from Edible Tubers of Dioscorea opposita cv. Nagaimo

    PubMed Central

    Chan, Yau Sang; Ng, Tzi Bun

    2013-01-01

    A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC50 values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

  5. Microbiological and biochemical profile of cv. Conservolea naturally black olives during controlled fermentation with selected strains of lactic acid bacteria.

    PubMed

    Panagou, Efstathios Z; Schillinger, Ulrich; Franz, Charles M A P; Nychas, George-John E

    2008-04-01

    The effect of controlled fermentation processes on the microbial association and biochemical profile of cv. Conservolea naturally black olives processed by the traditional anaerobic method was studied. The different treatments included (a) inoculation with a commercial starter culture of Lactobacillus pentosus, (b) inoculation with a strain of Lactobacillus plantarum isolated from a fermented cassava product and (c) uninoculated spontaneous process. Microbial growth, pH, titratable acidity, organic acids and volatile compounds were monitored throughout the fermentation. The initial microbiota consisted of Gram-negative bacteria, lactic acid bacteria and yeasts. Inhibition of Gram-negative bacteria was evident in all processes. Both starter cultures were effective in establishing an accelerated fermentation process and reduced the survival period of Gram-negative bacteria by 5 days compared with the spontaneous process, minimizing thus the likelihood of spoilage. Higher acidification of the brines was observed in inoculated processes without any significant difference between the two selected starter cultures (113.5 and 117.6mM for L. plantarum and L. pentosus, respectively). L. pentosus was also determined as the major species present during the whole process of spontaneous olive fermentation. It is characteristic that lactic acid fermentation was also initiated rapidly in the spontaneous process, as the conditions of fermentation, mainly the low salt level (6%, w/v) favored the dominance of lactic acid bacteria over yeasts. Lactic, acetic and propionic were the organic acids detected by HPLC in considerable amounts, whereas citric and malic acids were also present at low levels and degraded completely during the processes. Ethanol, methanol, acetaldehyde, ethyl acetate were the major volatile compounds identified by GC. Their concentrations varied among the different treatments, reflecting varying degrees of microbial activity in the brines. The results obtained from this study could help the Greek table olive industry to improve the existing processing schemes in order to increase product consistency and quality expanding the international market for naturally black olives. PMID:18206777

  6. Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.

    PubMed

    Dautt-Castro, Mitzuko; Ochoa-Leyva, Adrian; Contreras-Vergara, Carmen A; Pacheco-Sanchez, Magda A; Casas-Flores, Sergio; Sanchez-Flores, Alejandro; Kuhn, David N; Islas-Osuna, Maria A

    2015-01-01

    Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ? 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic process" among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening. PMID:25741352

  7. Structural analysis of wheat wax (Triticum aestivum, c.v. 'Naturastar' L.): from the molecular level to three dimensional crystals.

    PubMed

    Koch, K; Barthlott, W; Koch, S; Hommes, A; Wandelt, K; Mamdouh, W; De-Feyter, S; Broekmann, P

    2006-01-01

    In order to elucidate the self assembly process of plant epicuticular waxes, and the molecular arrangement within the crystals, re-crystallisation of wax platelets was studied on biological and non-biological surfaces. Wax platelets were extracted from the leaf blades of wheat (Triticum aestivum L., c.v. 'Naturastar', Poaceae). Waxes were analysed by gas chromatography (GC) and mass spectrometry (MS). Octacosan-1-ol was found to be the most abundant chemical component of the wax mixture (66 m%) and also the determining compound for the shape of the wax platelets. The electron diffraction pattern showed that both the wax mixture and pure octacosan-1-ol are crystalline. The re-crystallisation of the natural wax mixture and the pure octacosan-1-ol were studied by scanning tunnelling microscopy (STM), atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Crystallisation of wheat waxes and pure octacosano-1-ol on the non polar highly ordered pyrolytic graphite (HOPG) led to the formation of platelet structures similar to those found on the plant surface. In contrast, irregular wax morphologies and flat lying plates were formed on glass, silicon, salt crystals (NaCl) and mica surfaces. Movement of wheat wax through isolated Convallaria majalis cuticles led to typical wax platelets of wheat, arranged in the complex patterns typical for C. majalis. STM of pure octacosan-1-ol monolayers on HOPG showed that the arrangement of the molecules strictly followed the hexagonal structure of the substrate crystal. Re-crystallisation of wheat waxes on non-polar crystalline HOPG substrate showed that technical surfaces could be used to generate microstructured, biomimetic surfaces. AFM and SEM studies proved that a template effect of the substrate determined the orientation of the re-grown crystals. These effects of the structure and polarity of the substrate on the morphology of the epicuticular waxes are relevant for understanding interactions between biological as well as technical surfaces and waxes. PMID:16133211

  8. FANTINA: Fathom Asteroids Now: Tomography and Imagery of a NEA- Payload For Marco Polo R CV3 / ESA mission

    NASA Astrophysics Data System (ADS)

    Herique, A.; Biele, J.; Bousquet, P.; Ciarletti, V.; Ho, T. M.; Issler, J. L.; Kofman, W.; Michel, P.; Plettemeier, D.; Puget, P.; Souyris, J. C.; Ulamec, S.; van Zoest, T.; Zine, S.

    2012-04-01

    The internal structure of small bodies is still poorly known and has never been measured directly. There is no way to determine from ground based observation whether the body is a monolithic piece of rock or a rubble-pile, an aggregate of boulders held together by gravity and how much porosity it contains, both in the form of micro-scale or macro-scale porosity. Knowing this structure is a key point for a better understanding of the asteroid accretion and dynamical evolution. It is the main objective of the FANTINA experience proposed Payload for Marco Polo R CV3 / ESA mission: FANTINA (Marco Polo's Daughter) for Fathom Asteroids Now: Tomography and Imagery of a NEA is to provide measurement capabilities and science data which are not accessible by remote sensing of the asteroid from the Marco-Polo R spacecraft alone and which complement the analysis of the returned samples. The FANTINA payload is a Lander carrying the ASSERT experiment and a complementary payload to be defined: ASSERT is a bistatic CONSERT/Rosetta-like radar to achieve the tomography both in transmission and in reflexion of the asteroid in order to characterise its composition and its heterogeneity from decimetric to global scale. The lander is a long-lived bus in the 10-kg class derived from MASCOT/Hayabusa 2. This paper reviews the science rationale of FANTINA in the context of the Marco Polo R mission to 1996FG3. The surface package concept will be reviewed including the radar tomography principles. So a preliminary design and budget will be done.

  9. Effects of salicylic acid and putrescine on storability, quality attributes and antioxidant activity of plum cv. 'Santa Rosa'.

    PubMed

    Davarynejad, Gholam Hossein; Zarei, Mehdi; Nasrabadi, Mohamad Ebrahim; Ardakani, Elham

    2015-04-01

    Plum fruit has a short shelf life with a rapid deterioration in quality after harvest. The primary goal of this study is to investigate and compare the effect of putrescine and salicylic acid on quality properties and antioxidant activity of plum during storage. The plum fruits (cv. 'Santa Rosa') were harvested at the mature ripe stage, and dipped in different concentrations of putrescine (1, 2, 3 and 4 mmol/L) and salicylic acid (1, 2, 3 and 4 mmol/L), as well as distilled water (control) for 5 min. The fruits were then packed in boxes with polyethylene covers and stored at 4 °C with 95 % relative humidity for 25 days. A factorial trial based on completely randomized block design with 4 replications was carried out. The weight loss, fruit firmness, total soluble solids, titratable acidity, pH, maturity index, ascorbic acid, total phenolics and antioxidant activity at 0, 5, 10, 15, 20 and 25 days after harvest were recorded. During the storage period, the weight loss, total soluble solids, pH and maturity index increased significantly while the fruit firmness, titratable acidity, ascorbic acid, total phenolics and antioxidant activity decreased significantly (P?

  10. Genome organisation and retrotransposon driven molecular evolution of the endosperm Hardness (Ha) locus in Triticum aestivum cv Glenlea.

    PubMed

    Ragupathy, Raja; Cloutier, Sylvie

    2008-12-01

    Wheat endosperm texture is controlled primarily by a locus (Ha), which comprises Gsp-1, Pina and Pinb genes encoding the so-called grain softness protein, puroindoline-a and puroindoline-b, respectively. Pina and Pinb were detected only on the D-genome of hexaploid wheat and its diploid progenitors while Gsp-1 was on all three homoeologous loci. Hexaploid cultivar Glenlea has a hard phenotype due to a null Pina genotype (D-genome) but the sequence organization is not reported. This study aimed at understanding the evolution of homoeologous Ha loci. Sequencing of three BAC clones from cv Glenlea was performed and sequence analyses delimited the Ha loci which spanned 3,925, 5,330 and 31,607 bp in the A-, B- and D-genomes, respectively. A solo LTR of Angela retroelement, downstream to Gsp-A1 and a fragment of Sabrina retroelement, downstream of Gsp-B1, were discovered. We propose that the insertion of these elements into the intergenic regions have driven the deletions of genomic segments harbouring Pina and Pinb genes in the A- and B-genomes of hexaploid wheat. Similarly, fragments of Romani and Vagabond retroelements were identified between truncated Pina and Pinb genes, indicating their role in the deletion of Pina in Glenlea, leading to its hard texture. Structural differences of the Ha locus region of the A-genome between two hexaploid wheat varieties namely Glenlea and Renan (CR626929), suggested the presence of more than one tetraploid ancestor in the origin of hexaploid wheat. PMID:18830712

  11. Photosynthesis and growth responses of mustard (Brassica juncea L. cv Pusa Bold) plants to free air carbon dioxide enrichment (FACE).

    PubMed

    Ruhil, Kamal; Sheeba; Ahmad, Altaf; Iqbal, Muhammad; Tripathy, Baishnab C

    2014-12-01

    Increased atmospheric [CO2] is likely to affect photosynthesis, plant growth, and yield potential of plants. Mustard (Brassica juncea L.) is an important oil seed crop that is widely grown in India. Therefore, the impact of elevated [CO2] (585 ?mol mol(-1)) on pigment and protein content, chlorophyll a fluorescence, photosynthetic electron transport reactions, CO2 assimilation, biomass production, and seed yield potential was measured in B. juncea cv Pusa Bold, grown inside free air carbon dioxide enrichment (FACE) rings installed on the campus of Jawaharlal Nehru University, New Delhi, India. Plants were grown for three consecutive winter seasons (2010-2013), in ambient (385 ?mol mol(-1)) or elevated [CO2], in field conditions. Elevated [CO2] had no significant effect on the minimal chlorophyll fluorescence (F 0), while the quantum efficiency of Photosystem II, measured as variable fluorescence (F v?=?F m-F 0) to maximum fluoresence (F m), increased by 3 %. Electron transport rate, photosystem I, photosystem II, and whole chain electron transport rates increased by 8 % in elevated [CO2]. However, the net photosynthesis rate increased by ?50 % in three growing seasons under elevated [CO2] condition. The stomatal conductance and transpiration rate decreased resulting in higher photosynthetic water use efficiency. The photosynthesizing surface, i.e., leaf area index substantially increased leading to higher biomass and seed yield under elevated [CO2] condition. Acclimatory downregulation of photosynthesis and plant productivity was not observed in three consecutive growing years suggesting that in the absence of nutrient limitation, B. juncea is highly responsive to elevated CO2 whose yield potential shall increase in changing climatic conditions. PMID:25471475

  12. Pei Zhang CV INFORMATION

    E-print Network

    Rowe, Anthony

    Field, CA 94035 E-mail: peizhang@cmu.edu Phone: (609) 356-2525 http://www.ece.cmu.edu/~peizhang/ CURRENT on Smart Windows for Casual Collaborations $100,000. 2011 Nokia Inc. BodyNet: An Accurate Body Position,000. 2009-2011 Cylab/ARO. VMA: Dynamic and Secure Heterogeneous System for Elder Care. $150,000. 2009

  13. John Cushman's CV

    E-print Network

    F. Alejandro Bonilla

    Parashar*, R., and J. H. Cushman (2007) The finite-size Lyapunov exponent for ..... Cushman, J.H. (1980) Comparison of Convective and Diffusive Absorption of ... Dispersion: Angstroms to Kilometers, SIAM Conference on Mathematical and ...

  14. Efficacy of Pneumococcal Nontypable Haemophilus influenzae Protein D Conjugate Vaccine (PHiD-CV) in Young Latin American Children: A Double-Blind Randomized Controlled Trial

    PubMed Central

    Tregnaghi, Miguel W.; Sáez-Llorens, Xavier; López, Pio; Abate, Hector; Smith, Enrique; Pósleman, Adriana; Calvo, Arlene; Wong, Digna; Cortes-Barbosa, Carlos; Ceballos, Ana; Tregnaghi, Marcelo; Sierra, Alexandra; Rodriguez, Mirna; Troitiño, Marisol; Carabajal, Carlos; Falaschi, Andrea; Leandro, Ana; Castrejón, Maria Mercedes; Lepetic, Alejandro; Lommel, Patricia; Hausdorff, William P.; Borys, Dorota; Guiñazú, Javier Ruiz; Ortega-Barría, Eduardo; Yarzábal, Juan P.; Schuerman, Lode

    2014-01-01

    Background The relationship between pneumococcal conjugate vaccine–induced antibody responses and protection against community-acquired pneumonia (CAP) and acute otitis media (AOM) is unclear. This study assessed the impact of the ten-valent pneumococcal nontypable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) on these end points. The primary objective was to demonstrate vaccine efficacy (VE) in a per-protocol analysis against likely bacterial CAP (B-CAP: radiologically confirmed CAP with alveolar consolidation/pleural effusion on chest X-ray, or non-alveolar infiltrates and C-reactive protein ? 40 µg/ml); other protocol-specified outcomes were also assessed. Methods and Findings This phase III double-blind randomized controlled study was conducted between 28 June 2007 and 28 July 2011 in Argentine, Panamanian, and Colombian populations with good access to health care. Approximately 24,000 infants received PHiD-CV or hepatitis control vaccine (hepatitis B for primary vaccination, hepatitis A at booster) at 2, 4, 6, and 15–18 mo of age. Interim analysis of the primary end point was planned when 535 first B-CAP episodes, occurring ?2 wk after dose 3, were identified in the per-protocol cohort. After a mean follow-up of 23 mo (PHiD-CV, n?=?10,295; control, n?=?10,201), per-protocol VE was 22.0% (95% CI: 7.7, 34.2; one-sided p?=?0.002) against B-CAP (conclusive for primary objective) and 25.7% (95% CI: 8.4%, 39.6%) against World Health Organization–defined consolidated CAP. Intent-to-treat VE was 18.2% (95% CI: 5.5%, 29.1%) against B-CAP and 23.4% (95% CI: 8.8%, 35.7%) against consolidated CAP. End-of-study per-protocol analyses were performed after a mean follow-up of 28–30 mo for CAP and invasive pneumococcal disease (IPD) (PHiD-CV, n?=?10,211; control, n?=?10,140) and AOM (n?=?3,010 and 2,979, respectively). Per-protocol VE was 16.1% (95% CI: ?1.1%, 30.4%; one-sided p?=?0.032) against clinically confirmed AOM, 67.1% (95% CI: 17.0%, 86.9%) against vaccine serotype clinically confirmed AOM, 100% (95% CI: 74.3%, 100%) against vaccine serotype IPD, and 65.0% (95% CI: 11.1%, 86.2%) against any IPD. Results were consistent between intent-to-treat and per-protocol analyses. Serious adverse events were reported for 21.5% (95% CI: 20.7%, 22.2%) and 22.6% (95% CI: 21.9%, 23.4%) of PHiD-CV and control recipients, respectively. There were 19 deaths (n?=?11,798; 0.16%) in the PHiD-CV group and 26 deaths (n?=?11,799; 0.22%) in the control group. A significant study limitation was the lower than expected number of captured AOM cases. Conclusions Efficacy was demonstrated against a broad range of pneumococcal diseases commonly encountered in young children in clinical practice. Trial registration www.ClinicalTrials.gov NCT00466947 Please see later in the article for the Editors' Summary PMID:24892763

  15. 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV) induces memory B cell responses in healthy Kenyan toddlers.

    PubMed

    Muema, D M; Nduati, E W; Uyoga, M; Bashraheil, M; Scott, J A G; Hammitt, L L; Urban, B C

    2015-08-01

    Memory B cells are long-lived and could contribute to persistence of humoral immunity by maintaining the plasma-cell pool or making recall responses upon re-exposure to an antigen. We determined the ability of a pneumococcal conjugate vaccine to induce anti-pneumococcal memory B cells. Frequencies of memory B cells against pneumococcal capsular polysaccharides from serotypes 1, 6B, 14, 19F and 23F were determined by cultured B cell enzyme-linked immunospot (ELISPOT) in 35 children aged 12-23 months who received pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). The relationships between plasma antibodies and memory B cell frequencies were also assessed. After two doses of PHiD-CV, the proportion of subjects with detectable memory B cells against pneumococcal capsular polysaccharides increased significantly for serotypes 1 (3-45%; P?CV is capable of inducing memory B cells against some of the component pneumococcal capsular polysaccharides. PMID:25845628

  16. Tracing WR wind structures by using the orbiting companion in the 29d WC8d + O8-9IV binary CV Ser

    NASA Astrophysics Data System (ADS)

    David-Uraz, Alexandre; Moffat, Anthony F. J.; Chené, André Nicolas; Lange, Nicholas

    2011-01-01

    We have obtained continuous, high-precision, broadband visible photometry from the MOST satellite of CV Ser over more than a full orbit in order to link the small-scale light-curve variations to extinction due to wind structures in the WR component, thus permitting us to trace these structures. The light-curve presented unexpected characteristics, in particular eclipses with a varying depth. Parallel optical spectroscopy from the Mont Megantic Observatory and Dominion Astrophysical Observatory was obtained to refine the orbital and wind-collision parameters, as well as to reveal line emission from clumps.

  17. MOST: A Powerful Tool to Reveal the True Nature of the Mysterious Dust-Forming Wolf-Rayet Binary CV Ser

    NASA Astrophysics Data System (ADS)

    David-Uraz, A.; Moffat, A. F. J.; Chené, A.-N.; MOST Collaboration

    2012-12-01

    The WR + O binary CV Ser has been a source of mystery since it was shown that its atmospheric eclipses change with time over decades, in addition to its sporadic dust production. However, the first high-precision time-dependent photometric observations obtained with the MOST space telescope in 2009 show two consecutive eclipses over the 29 day orbit, with varying depths. A subsequent MOST run in 2010 showed a somewhat asymmetric eclipse profile. Parallel optical spectroscopy was obtained from the Observatoire du Mont-Mégantic (2009 and 2010) and from the Dominion Astrophysical Observatory (2009).

  18. Using the orbiting companion to trace WR wind structures in the 29d WC8d + O8-9IV binary CV Ser

    NASA Astrophysics Data System (ADS)

    David-Uraz, Alexandre; Moffat, Anthony F. J.

    2011-07-01

    We have used continuous, high-precision, broadband visible photometry from the MOST satellite to trace wind structures in the WR component of CV Ser over more than a full orbit. Most of the small-scale light-curve variations are likely due to extinction by clumps along the line of sight to the O companion as it orbits and shines through varying columns of the WR wind. Parallel optical spectroscopy from the Mont Megantic Observatory is used to refine the orbital and wind-collision parameters, as well as to reveal line emission from clumps.

  19. Sistema de Guiado Automtico para Altas Velocidades

    E-print Network

    Boyer, Edmond

    autónomo circulando en autopistas. Para ello, se ha desarrollado un sistema de control de dirección para conseguir un nuevo impulso en la política en materia de seguridad vial para la próxima década. Desde el

  20. Importantes avances para la nueva dramaturgia mexicana

    E-print Network

    Burgess, Ronald D.

    1985-10-01

    expresión para la élite que tenga el dinero para poderlo pagar.'' ENTRE LAS POLÍTICAS A SEGUIR "Que se les proporcione la asesoría de arquitectos en forma gratuita para que los sitios tengan una funcionalidad y seguridad necesarias. En cuanto al...

  1. Chemical composition, aroma evaluation, and oxygen radical absorbance capacity of volatile oil extracted from Brassica rapa cv. "yukina" used in Japanese traditional food.

    PubMed

    Usami, Atsushi; Motooka, Ryota; Takagi, Ayumi; Nakahashi, Hiroshi; Okuno, Yoshiharu; Miyazawa, Mitsuo

    2014-01-01

    The chemical composition of the volatile oil extracted from the aerial parts of Brassica rapa cv. "yukina" was analyzed using GC-MS, GC-PFPD, and GC-O. A total of 50 compounds were identified. The most prominent constituents were (E)-1,5-heptadiene (40.27%), 3-methyl-3-butenenitrile (25.97%) and 3-phenylpropanenitrile (12.41%). With regard to aroma compounds, 12 compounds were identified by GC-O analysis. The main aroma-active compounds were dimethyl tetrasulfide (sulphury-cabbage, FD = 64), 3-phenylpropanenitrile (nutty, FD = 64), 3-methylindole (pungent, FD = 64), and methional (potato, FD = 32). The antioxidant activity of the aroma-active compounds of the oil was determined using an oxygen radical absorbance capacity (ORAC) assay using fluorescein as the fluorescent probe. The ORAC values were found to be 785 ± 67 trolox equivalents (?mol TE/g) for B. rapa cv. "yukina" oil. The results obtained showed that the volatile oil extracted from the aerial parts is a good dietary source of antioxidants. PMID:24919480

  2. Safety and immunogenicity of 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Nigerian children: Booster dose and 2-dose catch-up regimens in the second year of life.

    PubMed

    Odusanya, Olumuyiwa O; Kuyinu, Yetunde A; Kehinde, Omolara A; Shafi, Fakrudeen; François, Nancy; Yarzabal, Juan Pablo; Dobbelaere, Kurt; Rüggeberg, Jens U; Borys, Dorota; Schuerman, Lode

    2014-01-01

    In a previous study, 3-dose primary vaccination of Nigerian infants with the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) was immunogenic for vaccine pneumococcal serotypes, with comparable tolerability between PHiD-CV and control groups. In an open-label study (ClinicalTrials.gov, NCT01153893), 68 primed children received a PHiD-CV booster dose co-administered with a diphtheria-tetanus-acellular pertussis (DTPa) booster dose at 15-21 months and 36 children unprimed for pneumococcal vaccination received two PHiD-CV catch-up doses (first dose co-administered with DTPa booster dose) at 15-21 and 17-23 months. Adverse events were recorded and immune responses were measured before and one month after vaccination. In both groups, pain was the most frequent solicited local symptom and fever was the most frequent solicited general symptom after the booster dose and each catch-up dose. Few grade 3 solicited symptoms and no vaccine-related serious adverse events were reported. After booster vaccination, for each vaccine serotype, at least 98.5% of children had an antibody concentration ? 0.2 µg/ml and at least 94.0% had an opsonophagocytic activity (OPA) titer ? 8. After 2-dose catch-up, for each vaccine serotype, at least 97.1% had an antibody concentration ? 0.2 µg/ml, except for serotypes 6B (82.9%) and 23F (88.6%), and at least 91.4% had an OPA titer ?8, except for serotypes 6B (77.4%) and 19F (85.3%). PHiD-CV induced antibody responses against protein D in both groups. In conclusion, PHiD-CV administered to Nigerian toddlers as a booster dose or 2-dose catch-up was well tolerated and immunogenic for vaccine pneumococcal serotypes and protein D. PMID:24356787

  3. Studies on the management of root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex of green gram, Vigna radiata cv ML-1108

    PubMed Central

    Haseeb, Akhtar; Sharma, Anita; Shukla, Prabhat Kuma

    2005-01-01

    Studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita–wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared to untreated inoculated plants. Analysis of data showed that carbofuran and A. indica seed powder increased plant growth and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens. PMID:16052706

  4. Studies on the management of root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex of green gram, Vigna radiata cv ML-1108.

    PubMed

    Haseeb, Akhtar; Sharma, Anita; Shukla, Prabhat Kumar

    2005-08-01

    Studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared to untreated inoculated plants. Analysis of data showed that carbofuran and A. indica seed powder increased plant growth and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens. PMID:16052706

  5. Competition for in vitro (/sup 3/H)gibberellin A/sub 4/ binding in cucumber by gibberellins and their derivatives. [Cucumis sativus L. cv National Pickling

    SciTech Connect

    Yalpani, N.; Srivastava, L.M.

    1985-12-01

    The gibberellin (GA) binding properties of a cytosol fraction from hypocotyls of cucumber (Cucumis sativus L. cv National Pickling) were examined using a DEAE filter paper assay, (/sup 3/H)GA/sub 4/, and over 20 GAs, GA derivatives and other growth regulators. The results demonstrate structural specificity of the binding protein for ..gamma..-lactonic C-19 GAs with a 3 ..beta..-hydroxyl and a C-6 carboxyl group. Additional hydroxylations of the A, C, or D ring of the ent-gibberellane skeleton and methylation of the C-6 carboxyl impede or abolish binding affinity. Bioassay data are generally supported by the in vitro results but significantly GA/sub 9/ and GA/sub 36/, both considered to be precursors of GA/sub 4/ in cucumber, show no affinity for the binding protein. The results are discussed in relation to the active site of the putative GA/sub 4/ receptor in cucumber.

  6. Effects of Arsenite, Sulfite, and Sulfate on Photosynthetic Carbon Metabolism in Isolated Pea (Pisum sativum L., cv Little Marvel) Chloroplasts 1

    PubMed Central

    Marques, Ivano A.; Anderson, Louise E.

    1986-01-01

    Photosynthetic CO2-fixation in isolated pea (Pisum sativum L., cv Little Marvel) chloroplasts during induction is markedly inhibited by 0.4 millimolar sulfite. Sulfate at the same concentration has almost no effect. The 14CO2-fixation pattern indicates that the primary effect of sulfite is inhibition of the reaction catalyzed by ribulose bisphosphate carboxylase and a stimulation of export of intermediates out of the chloroplasts. Inhibition of light modulation of stromal enzyme activity does not appear to account for the toxicity of SO2 in this Pisum variety. Arsenite at 0.2 millimolar concentrations inhibits light activation and inhibits photosynthetic CO2 fixation. The 14CO2-fixation pattern indicates that the primary effect of arsenite is inhibition of light activation of reductive pentose phosphate pathway enzyme activity. PMID:16665056

  7. Determination of replacement of some inorganic elements in pulvinus of bean (Phaseolus vulgaris cv. Gina 2004) at chilling temperature by the WDXRF spectroscopic technique

    NASA Astrophysics Data System (ADS)

    Dumlupinar, Rahmi; Demir, Faruk; Budak, Gokhan; Karabulut, Abdulhalik; Kadi, Nuray; Karakurt, Halil; Erdal, Serkan

    2007-01-01

    In this study, bean seedlings (Phaseolus vulgaris cv. Gina 2004) were exposed to chilling temperatures until leaves are wrinkled (9 day), that is, showed nyctinastic movement. Pulvinus were subsequently were cut from the leaves. Concentrations of inorganic elements (P, S, Cl, K, Ca, Cu) in the pulvinus were measured by wavelength-dispersive X-ray fluorescence (WDXRF) spectrometry. Results indicated that concentration change (%) was not significant for Ca (0.82) but it was significant for K, P, Cl, S, and especially Cu concentrations (5.4%, 12.8%, 40.2%, 43.7%, 365%, respectively) in pulvinus of plants exposed to chilling temperature compared with control group. We hypothesize here the presence of association between nyctinasti movement brought about by pulvinus at chilling temperature in bean and changes of K, P, Cl, S and especially Cu concentrations measured by WDXRF analysis method.

  8. On the use of Ethephon as abscising agent in cv. Crimson Seedless table grape production: combination of Fruit Detachment Force, Fruit Drop and metabolomics.

    PubMed

    Rizzuti, Antonino; Aguilera-Sáez, Luis Manuel; Gallo, Vito; Cafagna, Isabella; Mastrorilli, Piero; Latronico, Mario; Pacifico, Andrea; Matarrese, Angela Maria Stella; Ferrara, Giuseppe

    2015-03-15

    The effect of 2-chloroethylphosphonic acid (Ethephon, in the following ETH) as abscising agent on cv. Crimson Seedless table grape was investigated by means of Fruit Detachment Force (FDF) and Fruit Drop (FD) analyses combined with a metabolomic study carried out by High Resolution Mass Spectrometry (HRMS) and Nuclear Magnetic Resonance (NMR) spectroscopy. The effectiveness of ETH as abscising agent was ascertained with ETH concentration ranging from 1.4 to 4.0 g/L in a two-year study. The ETH treatments caused berry drops higher than 40% and induced an increase of tartaric acid, procyanidin P2, terpenoid derivatives and peonidin-3-glucoside as well as a decrease of catechin and epicatechin. HRMS-NMR covariance analysis was carried out to correlate the fluctuations of tartaric acid NMR signals to those of MS peaks of the secondary metabolites affected by ETH treatments. PMID:25308678

  9. A refractory inclusion in the Kaba CV3 chondrite - Some implications for the origin of spinel-rich objects in chondrites

    NASA Technical Reports Server (NTRS)

    Fegley, B., Jr.; Post, J. E.

    1985-01-01

    The first detailed petrographic and mineralogical study of a Ca, Al-rich inclusion (CAI) from the Kaba CV3 chondrite is reported. This 'fine-grained' CAI contains abundant small, rounded, rimmed, spinel-rich objects which have important features in common with the spinel-rich objects in other carbonaceous and ordinary chondrites. These nodules are interpreted as fractionated distillation residues of primitive dust. However, the available data do not unambiguously rule out a condensation origin for at least some of these objects. Finally, the preservation of distinct diopside-hedenbergite rims on the spinel-rich bodies and the small grain size of many minerals in the CAI matrix material both suggest that the CAI accreted cool and had a relatively cool thermal history in the Kaba parent body.

  10. 2012 Virginia Polytechnic Institute and State University CV-26NP Virginia Cooperative Extension programs and employment are open to all, regardless of race, color, national origin, sex, religion, age, disability, political beliefs, sexual orientation, or

    E-print Network

    Liskiewicz, Maciej

    2012 Virginia Polytechnic Institute and State University CV-26NP Virginia Cooperative Extension/affirmative action employer. Issued in furtherance of Cooperative Extension work, Virginia Polytechnic Institute and State University, Virginia State University, and the U.S. Department of Agriculture cooperating. Edwin J

  11. The model symbiotic association between Medicago truncatula cv. Jemalong and Rhizobium meliloti strain 2011 leads to N-stressed plants when symbiotic N2 fixation is the main N source for plant growth

    Microsoft Academic Search

    Delphine Moreau; Anne-Sophie Voisin; Christophe Salon; Nathalie Munier-Jolain

    2008-01-01

    A better knowledge of the nitrogen nutrition of Medicago truncatula at the whole plant level and its modula- tion by environmental factors is a crucial step to reach a complete understanding of legume nitrogen nutrition. This study was based on the symbiotic system that is the most commonly used by the research community (M. truncatula cv. Jemalong A173Rhizobium meliloti strain

  12. In my time at Strathclyde, I was given assistance by the University Careers service to boost my CV credentials and in 2006 I went on a work placement in Poland with the IAESTE (International Association for the Exchange

    E-print Network

    Mottram, Nigel

    In my time at Strathclyde, I was given assistance by the University Careers service to boost my CV the David Livingstone Centre for Sustainability. I have just completed the MRes IPPC programme, and I am a career in industry. I graduated from the MRes IPPC degree in November 2008, and in January 2009 I plan

  13. AYUDA PARA INVIDENTES UTILIZANDO TELEFONOS

    E-print Network

    Autonoma de Madrid, Universidad

    Aut´onoma de Madrid. El programa hace uso de informaci´on cartogr´afica y GPS. Permite llegar a sitios´es recorrerlas. La informaci´on que da el tel´efono son indicaciones por voz, aunque tambi´en se incluye informaci´on gr´afica para una persona con vista normal que realice la funci´on de acompa~nante o gu´ia. La

  14. Luteal maintenance of pregnancy in the African elephant (Loxodonta africana).

    PubMed

    Stansfield, F J; Allen, W R

    2012-06-01

    The ovaries of eight African elephant foetuses and their mothers between 2 and 22 months of gestation, and those of two cycling and two lactating elephants, were examined grossly, histologically and immunocytochemically, with emphasis on the development and regression of accessory corpora lutea (CL) of pregnancy and the steroidogenic capacities of the accessory CL and the foetal ovaries. The results supported recent findings that the accessory CL form as a result of luteinisation, with and without ovulation, of medium-sized follicles during the 3-week inter-luteal period of the oestrous cycle. They enlarge significantly and become steroidogenically active around 5 weeks of gestation, probably in response to the placental lactogen which is secreted by the implanting trophoblast of the conceptus. The large luteal cells stained strongly for 3? hydroxysteroid dehydrogenase (3?HSD) activity throughout the 22-month gestation period although they showed vacuolation and other degenerative changes in the final months of gestation coincident with hypertrophy and hyperplasia of 3?HSD-positive interstitial cells in the foetal gonads. It is proposed that the progestagens secreted by the enlarged gonads of the elephant foetus may function both to assist the maternal ovaries in supporting the pregnancy state and to induce torpor and intrauterine immobility of the rapidly growing foetus. PMID:22457432

  15. APPLICATION OF SPEECH RECOGNITION TO AFRICAN ELEPHANT (LOXODONTA AFRICANA) VOCALIZATIONS

    E-print Network

    Johnson, Michael T.

    vocalizations can be classified by human experts [4-6], few systems have been developed to automatically. The types of vocalizations are separated by animal behavior experts based on spectrogram analysis will outline a system used to perform both speaker identification and vocalization classification. The data

  16. Epidemiological aspects of Claviceps africana, causal agent of Sorghum ergot

    E-print Network

    Noe, Montes Garcia; Noe, Montes Garcia

    2005-02-17

    on 12 sphacelia that were held above soil surface for a year survived and infect sorghum florets on male-sterile line Host History and taxonomy A sorghum collection was first described by Linnaeus in 1753 under the name Holcus. However, in 1794...

  17. Dominance rank relationships among wild female African elephants, Loxodonta africana

    Microsoft Academic Search

    Elizabeth A. Archie; Thomas A. Morrison; Charles A. H. Foley; Cynthia J. Moss; Susan C. Alberts

    2006-01-01

    Socioecological models of the evolution of female-bonded societies predict a relation between resource dis- tribution and the nature of female affiliative and dominance relationships. Species that mainly rely on abundant, widely distributed resources, like African savanna elephants, are predicted to have unresolved dominance hierarchies and poorly differentiated female social relationships. Contrary to this prediction, female elephants have well-differentiated social relationships;

  18. From Africa of States to United Africa: Towards Africana Democracy

    E-print Network

    Danabo, Pelle Darota

    2008-08-21

    Since Western Liberal Democracy's philosophical, cultural, and value foundations are radically different from that of Africa and; based on post-disciplinary approach and review of the vast literature on theories and ...

  19. CHEMOSENSORY BEHAVIOR AND DEVELOPMENT OF AFRICAN MALE ELEPHANTS ( LOXODONTA AFRICANA )

    Microsoft Academic Search

    Kathryn R. Bagley

    African elephants are a polygynous species that raise offspring in a matriarchal society. Males disperse, spend time in male groups, and search for mates when mature. Urinary chemical signals play an important role in detecting reproductively active females. African male elephants develop movement, social and chemosensory behaviors over four major life changes. The first goal of this study was to

  20. Estudios ALCHEMIST para el cáncer de pulmón

    Cancer.gov

    Conjunto de información sobre los estudios ALCHEMIST para el cáncer de pulmón, los cuales examinarán los tejidos de tumores de pacientes con cáncer de pulmón en estadio inicial para detectar mutaciones genéticas en genes EGFR y ALK.

  1. Festival del Sur-Encuentro Teatral Tres Continentes: Un festival consolidado

    E-print Network

    Má rquez Montes, Carmen

    1995-10-01

    Marruecos; las islas, conquistadas por los españoles en las mismas fechas del descubrimiento de América, siempre fueron lugar de paso para América; de ahí que Canarias sea europea por mera cuestión de mapa político, africana por situación geográfica y...

  2. Fundo Global para o Meio Ambiente

    E-print Network

    #12;#12;Fundo Global para o Meio Ambiente Global Environment Facility Avaliação dos Recursos de Climáticos Center for Weather Forecasts and Climate Studies Programa das Nações Unidas para o Meio Ambiente Ambiente - PNUMA Fundo Global para o Meio Ambiente (Global Environment Facility) - GEF Projeto SWERA

  3. Proyecto de Investigacin (Proyecto para Jovenes Investigadores)

    E-print Network

    Mejia-Alvarez, Pedro

    de nuevos modelos, métodos de planificación y herramientas para el diseño y validación de sistemas deProyecto de Investigación (Proyecto para Jovenes Investigadores) Planificación de Sistemas de Resumen Los sistemas de tiempo real se utilizan para controlar y monitorizar procesos industriales

  4. inter-establecimientos de investigacin para

    E-print Network

    sociales, en una perspectiva de desarrollo sostenible. La agencia es autoridad contratante delegada paraL'Agencia inter-establecimientos de investigación para el desarrollo La AIRD: una agencia AIRD, agencia inter-establecimientos de investigación para el desarrollo1 , fue creada oficialmente por

  5. Protocolo de espacios para unidades externas 1 PROTOCOLO DE ESPACIOS PARA PERSONAL Y

    E-print Network

    Escolano, Francisco

    Protocolo de espacios para unidades externas 1 PROTOCOLO DE ESPACIOS PARA PERSONAL Y ESTANCIAS DE UNIDADES EXTERNAS INDICE: ­ Acceso al protocolo de espacios para externos ­ ¿Cómo acceder al protocolo? ­ Solicitud de clave ­ Pasos necesarios para cumplimentar el protocolo de espacios ­ Página principal del

  6. REE and Hf distribution among mineral phases in the CV-CK clan: A way to explain present-day Hf isotopic variations in chondrites

    NASA Astrophysics Data System (ADS)

    Martin, Céline; Debaille, Vinciane; Lanari, Pierre; Goderis, Steven; Vandendael, Isabelle; Vanhaecke, Frank; Vidal, Olivier; Claeys, Philippe

    2013-11-01

    Chondrites are among the most primitive objects in the Solar System and constitute the main building blocks of telluric planets. Among the radiochronometers currently used for dating geological events, Sm-Nd and Lu-Hf are both composed of refractory, lithophile element. They are thought to behave similarly as the parent elements (Sm and Lu) are generally less incompatible than the daughter elements (Nd and Hf) during geological processes. As such, their respective average isotopic compositions for the solar system should be well defined by the average of chondrites, called Chondritic Uniform Reservoir (CHUR). However, while the Sm-Nd isotopic system shows an actual spread of less than 4% in the average chondritic record, the Lu-Hf system shows a larger variation range of 28% [Bouvier A., Vervoort J. D. and Patchett P. J. (2008) The Lu-Hf and Sm-Nd isotopic composition of CHUR: Constraints from unequilibrated chondrites and implications for the bulk composition of terrestrial planets. Earth Planet. Sci. Lett.273, 48-57]. To better understand the contrast between Sm-Nd and Lu-Hf systems, the REE and Hf distribution among mineral phases during metamorphism of Karoonda (CK) and Vigarano-type (CV) carbonaceous chondrites has been examined. Mineral modes were determined from elemental mapping on a set of five CK chondrites (from types 3-6) and one CV3 chondrite. Trace-element patterns are obtained for the first time in all the chondrite-forming minerals of a given class (CK chondrites) as well as one CV3 sample. This study reveals that REE are distributed among both phosphates and silicates. Only 30-50% of Sm and Nd are stored in phosphates (at least in chondrites types 3-5); as such, they are not mobilized during early stages of metamorphism. The remaining fraction of Sm and Nd is distributed among the same mineral phases; these elements are therefore not decoupled during metamorphism. Of the whole-rock total of Lu, the fraction held in phosphate decreases significantly as the degree of metamorphism increases (30% for types 3 and 4, less than 5% in type 6). In contrast to Lu, Hf is mainly hosted by silicates with little contribution from phosphates throughout the CK metamorphic sequence. A significant part of Sm and Nd are stored in phosphates in types 3-5, and these elements behave similarly during CK chondrite metamorphism. That explains the robustness of the Sm/Nd ratios in chondrites through metamorphism, and the slight discrepancies observed in the present-day isotopic Nd values in chondrites. On the contrary, Lu and Hf are borne by several different minerals and consequently they are redistributed during metamorphism-induced recrystallization. The Lu/Hf ratios are therefore significantly disturbed during chondrites metamorphism, leading to the high discrepancies observed in present-day Hf isotopic values in chondrites.

  7. Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy

    PubMed Central

    Murphy, Sandra; Henry, Michael; Meleady, Paula; Zweyer, Margit; Mundegar, Rustam R.; Swandulla, Dieter; Ohlendieck, Kay

    2015-01-01

    In skeletal muscle, the dystrophin-glycoprotein complex forms a membrane-associated assembly of relatively low abundance, making its detailed proteomic characterization in normal versus dystrophic tissues technically challenging. To overcome this analytical problem, we have enriched the muscle membrane fraction by a minimal differential centrifugation step followed by the comprehensive label-free mass spectrometric analysis of microsomal membrane preparations. This organelle proteomic approach successfully identified dystrophin and its binding partners in normal versus dystrophic hind limb muscles. The introduction of a simple pre-fractionation step enabled the simultaneous proteomic comparison of the reduction in the dystrophin-glycoprotein complex and secondary changes in the mdx-4cv mouse model of dystrophinopathy in a single analytical run. The proteomic screening of the microsomal fraction from dystrophic hind limb muscle identified the full-length dystrophin isoform Dp427 as the most drastically reduced protein in dystrophinopathy, demonstrating the remarkable analytical power of comparative muscle proteomics. Secondary pathoproteomic expression patterns were established for 281 proteins, including dystrophin-associated proteins and components involved in metabolism, signalling, contraction, ion-regulation, protein folding, the extracellular matrix and the cytoskeleton. Key findings were verified by immunoblotting. Increased levels of the sarcolemmal Na+/K+-ATPase in dystrophic leg muscles were also confirmed by immunofluorescence microscopy. Thus, the reduction of sample complexity in organelle-focused proteomics can be advantageous for the profiling of supramolecular protein complexes in highly intricate systems, such as skeletal muscle tissue. PMID:26067837

  8. Effect of a chronic and moderate ozone pollution on the phenolic pattern of bean leaves (Phaseolus vulgaris L. cv Nerina): relations with visible injury and biomass production.

    PubMed

    Kanoun, M; Goulas, M J.P.; Biolley, J -P.

    2001-05-01

    From sowing, bean (Phaseolus vulgaris L. cv Nerina) plants were exposed to three chronic doses of ozone for 7h.day(-1): non-filtered air (NF), non-filtered air supplied with 40nl.l(-1) ozone (NF+40) and non-filtered air supplied with 60nll(-1) ozone (NF+60). Four harvests were carried out 6, 13, 20 and 27 days after emergence. Either primary leaves, or first trifoliate leaves, or both were sampled as far as possible. For each sampled leaf, visible ozone injuries were registered, the free polyphenolic pool was analysed using HPLC and the dry matter was weighed. Visible damage on leaves was related to both exposure time and ozone concentration added. There were no adverse effects of added ozone on the biomass of primary leaves while a significant reduction of first trifoliates dry matter could be observed (NF+60 atmosphere, third and fourth harvest). Among the normally occurring phenolics, we detected a significant decrease in the accumulation of a hydroxycinnamic acid derivative as the ozone concentration increased. Nevertheless, we demonstrated that this ozone-induced modification could be sometimes distinguishable with difficulties from changes expected to be of development relevance. Beside this phenolic disbalance, we detected a de novo biosynthesis of compounds that closely depended on the level of visible ozone injury. Since their accumulation increased with leaf damage, these ozone-induced phenolics could be used to detect phytotoxic ambient levels of tropospheric ozone. PMID:11274768

  9. Isolation of a Hemagglutinin with Potent Antiproliferative Activity and a Large Antifungal Defensin from Phaseolus vulgaris cv. Hokkaido Large Pinto Beans.

    PubMed

    Yin, Cuiming; Wong, Jack Ho; Ng, Tzi Bun

    2015-06-10

    Lectins (hemagglutinins) are defined as sugar-binding proteins or glycoproteins with various biological activities. A 60 kDa dimeric hemagglutinin with a blocked N-terminus was isolated in large yield (190 mg/60 g) from the common edible bean Phaseolus vulgaris cv. Hokkaido large pinto bean. Its hemagglutinating, antifungal, and antitumor activities as well as the effects of carbohydrate and metal ions on its hemagglutinating activity were examined. It inhibited the proliferation of nasopharyngeal carcinoma (CNE2), human breast cancer (MCF7), and hepatoma (HepG2) cells. The IC50 values toward HepG2, MCF7, and CNE2 cells after treatment for 48 h were 8.1, 6.07, and 7.49 ?M, respectively, which were relatively low among lectins of different P. vulgaris cultivars. From the pinto beans, a 10888 Da antifungal peptide with similarity to plant defensins as revealed by mass spectroscopic analysis was also isolated with a yield of 3.2 mg of proteins from 60 g of beans. The large defensin was capable of inhibiting mycelial growth in Mycosphaerella arachidicola, Setosphaeria turcica, Bipolaris maydis, and Fusarium oxysporum but not in Valsa mali. PMID:25965006

  10. Inducing rye 1R chromosome structural changes in common wheat cv. Chinese spring by the gametocidal chromosome 2C of Aegilops cylindrica.

    PubMed

    Shi, Fang; Liu, Kun-Fan; Endo, Takashi R; Wang, Dao-Wen

    2005-05-01

    To generate 1 R deletion and translocation lines, we introduced a 2C chromosome,which was derived from Aegilops cylindrica and was known to have a gametocidal function when added monosomically into common wheat cv. Chinese Spring (CS) and its derivative, into a wheat-rye 1R chromosome disomic addition line (CS-1R"). When the individuals with chromosome constitution 21" + 1R" + 2C' (2n = 45) were selfed, the 1R chromosome structural changes were found to be induced with high frequency (24.1%) among the progenies. By using C-banding and GISH analysis, we analyzed 1R structural changes in 46 F3 individuals, which came from 23 F2 plants. The rearranged 1R chromosomes could be characterized in about 85% of the F3 individuals. This included telosome 1RL (39.1%), iso-chromosome 1 RL (2.2%), whole arm translocation involving 1RL (32.6%), telosome 1RS (4.3%), iso-chromosome 1RS (4.3%), and 1R deletion mutant with break point in the long arm (2.2%). The mutant 1R lines obtained in this study will potentially be useful in mapping the chromosome locations of agronomically important genes located in 1R. This study also demonstrated that molecular markers might be used to identify wheat chromosome arm involved in translocation with 1R. PMID:16018259

  11. Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy.

    PubMed

    Murphy, Sandra; Henry, Michael; Meleady, Paula; Zweyer, Margit; Mundegar, Rustam R; Swandulla, Dieter; Ohlendieck, Kay

    2015-01-01

    In skeletal muscle, the dystrophin-glycoprotein complex forms a membrane-associated assembly of relatively low abundance, making its detailed proteomic characterization in normal versus dystrophic tissues technically challenging. To overcome this analytical problem, we have enriched the muscle membrane fraction by a minimal differential centrifugation step followed by the comprehensive label-free mass spectrometric analysis of microsomal membrane preparations. This organelle proteomic approach successfully identified dystrophin and its binding partners in normal versus dystrophic hind limb muscles. The introduction of a simple pre-fractionation step enabled the simultaneous proteomic comparison of the reduction in the dystrophin-glycoprotein complex and secondary changes in the mdx-4cv mouse model of dystrophinopathy in a single analytical run. The proteomic screening of the microsomal fraction from dystrophic hind limb muscle identified the full-length dystrophin isoform Dp427 as the most drastically reduced protein in dystrophinopathy, demonstrating the remarkable analytical power of comparative muscle proteomics. Secondary pathoproteomic expression patterns were established for 281 proteins, including dystrophin-associated proteins and components involved in metabolism, signalling, contraction, ion-regulation, protein folding, the extracellular matrix and the cytoskeleton. Key findings were verified by immunoblotting. Increased levels of the sarcolemmal Na+/K+-ATPase in dystrophic leg muscles were also confirmed by immunofluorescence microscopy. Thus, the reduction of sample complexity in organelle-focused proteomics can be advantageous for the profiling of supramolecular protein complexes in highly intricate systems, such as skeletal muscle tissue. PMID:26067837

  12. Characterization of Growth, Water Relations, and Proline Accumulation in Sodium Sulfate Tolerant Callus of Brassica napus L. cv Westar (Canola) 1

    PubMed Central

    Chandler, Stephen F.; Thorpe, Trevor A.

    1987-01-01

    Unselected and sodium sulfate tolerant callus cultures of Brassica napus L. cv Westar were grown on media supplemented with mannitol, NaCl, or Na2SO4. In all cases, growth of tolerant callus, measured on a fresh weight or dry weight basis, was greater than that of unselected callus, which was also subject to necrosis on high levels of salt. Tissue water potential became more negative in both unselected and tolerant callus grown in the presence of mannitol or Na2SO4. Water potentials in unselected callus were more negative than those of the tolerant tissues; but over a range of Na2SO4 concentrations both cultures displayed osmotic adjustment, maintaining relatively constant turgor. Proline accumulation in both unselected and tolerant callus was low (15 to 20 micromoles per gram dry weight) in the absence of stress, but increased on media supplemented with mannitol, NaCl, or Na2SO4. Increases in proline concentration were approximately linear in tolerant callus, reaching a maximum of 130 to 175 micromoles per gram dry weight. In unselected callus, concentrations were higher, reaching 390 to 520 micromoles per gram dry weight. Proline accumulation was correlated with inhibition of growth, and there was a negative correlation between proline concentration and culture age for tolerant callus. PMID:16665381

  13. The F-box protein COI1 functions upstream of MYB305 to regulate primary carbohydrate metabolism in tobacco (Nicotiana tabacum L. cv. TN90)

    PubMed Central

    Zhang, Hongbo

    2014-01-01

    Jasmonate (JA) plays an important role in regulating plant male fertility and secondary metabolism, but its role in regulating primary metabolism remains unclear. The F-box protein CORONATINE INSENSITIVE 1 (COI1) is a critical component of the JA receptor, and mediates JA-signalling by targeting JASMONATE ZIM-domain (JAZ) proteins for proteasomal degradation in response to JA perception. Here, we found that RNA interference-mediated knockdown of NtCOI1 in tobacco (Nicotiana tabacum L. cv. TN90) recapitulated many previously observed phenotypes in coi1 mutants, including male sterility, JA insensitivity, and loss of floral anthocyanin production. It also affected starch metabolism in the pollen, anther wall, and floral nectary, leading to pollen abortion and loss of floral nectar. Transcript levels of genes encoding starch metabolism enzymes were significantly altered in the pollen, anther wall, and floral nectary of NtCOI1-silenced tobacco. Changes in leaf primary metabolism were also observed in the NtCOI1-silenced tobacco. The expression of NtMYB305, an orthologue of MYB305 previously identified as a flavonoid metabolic regulator in Antirrhinum majus flowers and as a floral-nectar regulator mediating starch synthesis in ornamental tobacco, was extremely downregulated in NtCOI1-silenced tobacco. These findings suggest that NtCOI1 functions upstream of NtMYB305 and plays a fundamental role in coordinating plant primary carbohydrate metabolism and correlative physiological processes. PMID:24604735

  14. Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway.

    PubMed

    Ritala, Anneli; Dong, Lemeng; Imseng, Nicole; Seppänen-Laakso, Tuulikki; Vasilev, Nikolay; van der Krol, Sander; Rischer, Heiko; Maaheimo, Hannu; Virkki, Arho; Brändli, Johanna; Schillberg, Stefan; Eibl, Regine; Bouwmeester, Harro; Oksman-Caldentey, Kirsi-Marja

    2014-04-20

    The terpenoid indole alkaloids are one of the major classes of plant-derived natural products and are well known for their many applications in the pharmaceutical, fragrance and cosmetics industries. Hairy root cultures are useful for the production of plant secondary metabolites because of their genetic and biochemical stability and their rapid growth in hormone-free media. Tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots, which do not produce geraniol naturally, were engineered to express a plastid-targeted geraniol synthase gene originally isolated from Valeriana officinalis L. (VoGES). A SPME-GC-MS screening tool was developed for the rapid evaluation of production clones. The GC-MS analysis revealed that the free geraniol content in 20 hairy root clones expressing VoGES was an average of 13.7 ?g/g dry weight (DW) and a maximum of 31.3 ?g/g DW. More detailed metabolic analysis revealed that geraniol derivatives were present in six major glycoside forms, namely the hexose and/or pentose conjugates of geraniol and hydroxygeraniol, resulting in total geraniol levels of up to 204.3 ?g/g DW following deglycosylation. A benchtop-scale process was developed in a 20-L wave-mixed bioreactor eventually yielding hundreds of grams of biomass and milligram quantities of geraniol per cultivation bag. PMID:24530945

  15. Identification, synthesis, and characterization of novel sulfur-containing volatile compounds from the in-depth analysis of Lisbon lemon peels (Citrus limon L. Burm. f. cv. Lisbon).

    PubMed

    Cannon, Robert J; Kazimierski, Arkadiusz; Curto, Nicole L; Li, Jing; Trinnaman, Laurence; Ja?czuk, Adam J; Agyemang, David; Da Costa, Neil C; Chen, Michael Z

    2015-02-25

    Lemons (Citrus limon) are a desirable citrus fruit grown and used globally in a wide range of applications. The main constituents of this sour-tasting fruit have been well quantitated and characterized. However, additional research is still necessary to better understand the trace volatile compounds that may contribute to the overall aroma of the fruit. In this study, Lisbon lemons (C. limon L. Burm. f. cv. Lisbon) were purchased from a grove in California, USA, and extracted by liquid-liquid extraction. Fractionation and multidimensional gas chromatography-mass spectrometry were utilized to separate, focus, and enhance unidentified compounds. In addition, these methods were employed to more accurately assign flavor dilution factors by aroma extract dilution analysis. Numerous compounds were identified for the first time in lemons, including a series of branched aliphatic aldehydes and several novel sulfur-containing structures. Rarely reported in citrus peels, sulfur compounds are known to contribute significantly to the aroma profile of the fruit and were found to be aroma-active in this particular study on lemons. This paper discusses the identification, synthesis, and organoleptic properties of these novel volatile sulfur compounds. PMID:25639384

  16. Developmental patterns of emission of scent compounds and related gene expression in roses of the cultivar Rosa x hybrida cv. 'Yves Piaget'.

    PubMed

    Chen, Xiaomin; Baldermann, Susanne; Cao, Shuyan; Lu, Yao; Liu, Caixia; Hirata, Hiroshi; Watanabe, Naoharu

    2015-02-01

    2-Phenylethanol (2PE) and 3,5-dimethoxytoluene (DMT) are characteristic scent compounds in specific roses such as Rosa x hybrida cv. 'Yves Piaget'. We analyzed the endogenous concentrations and emission of 2PE and DMT during the unfurling process in different floral organs, as well as changes in transcript levels of the two key genes, PAR and OOMT2. The emission of both 2PE and DMT increased during floral development to reach peaks at the fully unfurled stage. The relative transcripts of PAR and OOMT2 also increased during floral development. Whereas the maximum for OOMT2 was found at the fully unfurled stage (stage 4), similar expression levels of PAR were detected at stage 4 and the senescence stage (stage 6). The results demonstrate a positive correlation between the expression levels of PAR and OOMT2 and the emission of 2PE and DMT. In addition, endogenous volatiles and relative transcripts showed tissue- and development-specific patterns. PMID:25576838

  17. Characterization, Purification of Poncirin from Edible Citrus Ougan (Citrus reticulate cv. Suavissima) and Its Growth Inhibitory Effect on Human Gastric Cancer Cells SGC-7901

    PubMed Central

    Zhu, Xiaoyan; Luo, Fenglei; Zheng, Yixiong; Zhang, Jiukai; Huang, Jianzhen; Sun, Chongde; Li, Xian; Chen, Kunsong

    2013-01-01

    Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit. PMID:23615464

  18. [Effects of interspecies difference of arbuscular mycorrhizal fungi on Citrus grandis cv. Changshou Shatian you seedlings vegetative growth and mineral contents].

    PubMed

    Tong, Ruijian; Yang, Xiaohong; Li, Dongyan

    2006-07-01

    In a pot experiment, axenic pomelo (Citrus grandis cv. Changshou Shatian You) seedlings were inoculated with arbuscular mycorrhizal (AM) fungi Gigaspora margarita, Glomus mosseae and Glomus versiforme, respectively, and their vegetative growth and mineral contents were studied. The results showed that the seedlings inoculated with test AM fungi could be infected effectively, and their shoot and root growth, especially fibrous root growth, was significantly improved, compared with the control. After inoculated with G. mosseae, the root length, lateral root number, and fibrous root number and length of the seedlings were increased by 22.7%, 35.7%, 160. 8% and 103.2%, respectively. The inoculation of these three AM fungi could significantly elevate the N, P, K, Ca, Mg, Zn, Cu and Mn contents in seedlings leaves. For example, the P content was increased by 46.8%, 88. 7% and 32. 3% , respectively, compared with the control. Among the three treatments, the seedlings inoculated with G. mosseae had the best vegetative growth, and the highest mycorrhizal dependence and N, P, K, Ca, Zn and Cu contents. The relative occurrence of AM fungal species decreased in the order of G. mosseae > G. margarita > G. versiforme, indicating that G. mosseae was most suitable for C. grandis seedlings inoculation. PMID:17044497

  19. Molecular cloning and partial characterization of a peroxidase gene expressed in the roots of Portulaca oleracea cv., one potentially useful in the remediation of phenolic pollutants.

    PubMed

    Matsui, Takeshi; Nomura, Yuki; Takano, Mai; Imai, Sofue; Nakayama, Hideki; Miyasaka, Hitoshi; Okuhata, Hiroshi; Tanaka, Satoshi; Matsuura, Hideyuki; Harada, Kazuo; Bamba, Takeshi; Hirata, Kazumasa; Kato, Ko

    2011-01-01

    Portulaca (Portulaca oleracea cv.) efficiently removes phenolic pollutants from hydroponic solution. In plant roots, peroxidase (PRX) is thought to be involved in the removal of phenolic pollutants by the cross-linking them to cell wall polysaccharides or proteins at the expense of reduction of hydrogen peroxide (H(2)O(2)). In this study, we found that portulaca roots secreted an acidic PRX isozyme that had relatively high H(2)O(2) affinity. We isolated five PRX genes, and the recombinant PRX proteins produced in cultured tobacco cells were partially characterized. Among these genes, PoPRX2 probably encoded the acidic PRX isozyme. PoPRX2 had an extra N-terminal region which has not been reported for other PRX proteins. We found that PoPRX2 oxidized phenolic pollutants, including bisphenol A, octylphenol, nonylphenol, and 17?-estradiol. In addition, we found that the Cys261 residue of PoPRX2 played an important role in the determination of affinity for H(2)O(2) and stability toward H(2)O(2). PMID:21597193

  20. Effect of boiling time on chemical composition and physico-functional properties of flours from taro (Colocasia esculenta cv fouê) corm grown in Côte d'Ivoire.

    PubMed

    Amon, Anon Simplice; Soro, René Yadé; Assemand, Emma Fernande; Dué, Edmond Ahipo; Kouamé, Lucien Patrice

    2014-05-01

    Taro (Colocasia esculenta cv fouê) corm was subjected to different boiling times and the changes in chemical composition and physico-functional properties were investigated using standard method. The change in boiling time led to a significant (P?

  1. A peroxisomally localized acyl-activating enzyme is required for volatile benzenoid formation in a Petunia×hybrida cv. ‘Mitchell Diploid’ flower

    PubMed Central

    2012-01-01

    Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petunia×hybrida cv. ‘Mitchell Diploid’ (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway intermediate by a coenzyme A (CoA) dependent, ?-oxidative scheme. Metabolic flux analysis, reverse genetics, and biochemical characterizations of key enzymes in this pathway have supported this putative concept. However, the theoretical first enzymatic reaction, which leads to the production of cinnamoyl-CoA, has only been physically demonstrated in a select number of bacteria like Streptomyces maritimus through mutagenesis and recombinant protein production. A transcript hasbeen cloned and characterized from MD flowers that shares high homology with an Arabidopsis thaliana transcript ACYL-ACTIVATING ENZYME11 (AtAAE11) and the S. maritimus ACYL-COA:LIGASE (SmEncH). In MD, the PhAAE transcript accumulates in a very similar manner as bona fide FVBP network genes, i.e. high levels in an open flower petal and ethylene regulated. In planta, PhAAE is localized to the peroxisome. Upon reduction of PhAAE transcript through a stable RNAi approach, transgenic flowers emitted a reduced level of all benzenoid volatile compounds. Together, the data suggest that PhAAE may be responsible for the activation of t-cinnamic acid, which would be required for floral volatile benzenoid production in MD. PMID:22771854

  2. New coumarins from the roots of Angelica dahurica var. formosana cv. Chuanbaizhi and their inhibition on NO production in LPS-activated RAW264.7 cells.

    PubMed

    Deng, Gai-Gai; Wei, Wei; Yang, Xiu-Wei; Zhang, You-Bo; Xu, Wei; Gong, Ning-Bo; Lü, Yang; Wang, Feng-Feng

    2015-03-01

    A new linear pyranocoumarin named (-)-hydroxydecursinol (1) and a new biscoumarin named (±)-dahuribiscoumarin (2), together with six known compounds isoimperatorin (3), imperatorin (4), phellopterin (5), isodemethylfuropinarine (6), demethylfuropinarine (7), and (+)-decursinol (8) were isolated from the 75% ethanolic extract of the roots of Angelica dahurica var. formosana cv. Chuanbaizhi. Their structures were elucidated by extensive spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry, and the structure of 2 was confirmed by single-crystal X-ray diffraction. All of the isolated compounds were evaluated for the inhibition against nitric oxide (NO) production in the lipopolysaccharide (LPS)-activated RAW264.7 macrophage cell line, and exhibited the inhibitory activity on NO production in a concentration-dependent manner. Furthermore, real-time PCR analysis revealed that compounds 2, 5-8 could significantly suppress the expression levels of inducible nitric oxide synthase mRNA in a concentration-dependent manner. And their primary structure-activity relationships of NO inhibitory effects were also briefly discussed. These compounds are potential candidates for further bioassay studies to determine their suitability as drug leads. PMID:25647326

  3. Arbuscular mycorrhizal fungi enhance fruit growth and quality of chile ancho (Capsicum annuum L. cv San Luis) plants exposed to drought.

    PubMed

    Mena-Violante, Hortencia G; Ocampo-Jiménez, Omar; Dendooven, Luc; Martínez-Soto, Gerardo; González-Castañeda, Jaquelina; Davies, Fred T; Olalde-Portugal, Víctor

    2006-06-01

    The effect of arbuscular mycorrhizal fungi (AMF) and drought on fruit quality was evaluated in chile ancho (Capsicum annuum L. cv San Luis). AMF treatments were (1) Glomus fasciculatum (AMFG), (2) a fungal species consortium from the forest "Los Tuxtla" in Mexico (AMFT), (3) a fungal species consortium from the Sonorian desert in Mexico (AMFD), and (4) a noninoculated control (NAMF). Plants were exposed to a 26-day drought cycle. Fruit quality was determined by measuring size (length, width, and pedicel length), color, chlorophyll, and carotenoid concentration. Under nondrought conditions, AMFG produced fruits that were 13% wider and 15% longer than the NAMF treatment. Under nondrought conditions, fruit fresh weight was 25% greater in the AMFG treatment compared to the NAMF. Under drought, fruits in the AMFT and AMFD treatments showed fresh weights similar to those in the NAMF treatment not subjected to drought. Fruits of the AMFG treatment subjected to drought showed the same color intensity and chlorophyll content as those of the nondroughted NAMF treatment and carotenoid content increased 1.4 times compared to that in the NAMF not exposed to drought. It is interesting to note that fruits in the AMFD treatment subjected to drought and the NAMF treatment not exposed to drought reached the same size. AMFD treatment increased the concentration of carotenes (1.4 times) under nondrought conditions and the concentration of xanthophylls (1.5 times) under drought when compared to the nondroughted NAMF treatment. PMID:16741758

  4. Relationships between harvest time and wine composition in Vitis vinifera L. cv. Cabernet Sauvignon 2. Wine sensory properties and consumer preference.

    PubMed

    Bindon, Keren; Holt, Helen; Williamson, Patricia O; Varela, Cristian; Herderich, Markus; Francis, I Leigh

    2014-07-01

    A series of five Vitis vinifera L. cv Cabernet Sauvignon wines were produced from sequentially-harvested grape parcels, with alcohol concentrations between 12% v/v and 15.5% v/v. A multidisciplinary approach, combining sensory analysis, consumer testing and detailed chemical analysis was used to better define the relationship between grape maturity, wine composition and sensory quality. The sensory attribute ratings for dark fruit, hotness and viscosity increased in wines produced from riper grapes, while the ratings for the attributes red fruit and fresh green decreased. Consumer testing of the wines revealed that the lowest-alcohol wines (12% v/v) were the least preferred and wines with ethanol concentration between 13% v/v and 15.5% v/v were equally liked by consumers. Partial least squares regression identified that many sensory attributes were strongly associated with the compositional data, providing evidence of wine chemical components which are important to wine sensory properties and consumer preferences, and which change as the grapes used for winemaking ripen. PMID:24518320

  5. Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK

    PubMed Central

    Kimura, Hirokazu; Tsukagoshi, Hiroyuki; Kozawa, Kunihisa; Koketsu, Mamoru; Ninomiya, Masayuki; Furukawa, Shoei

    2013-01-01

    Components from Dendranthema × grandiflorum cv. “Mottenohoka” that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth. PMID:23554829

  6. The sequence of change within the photosynthetic apparatus of wheat following short-term exposure to ozone. [Triticum aestivum L. cv Avalon

    SciTech Connect

    Farage, P.K.; Long, S.P.; Baker, N.R. (Univ. of Essex (England)); Lechner, E.G. (Univ. of Vienna (Austria))

    1991-02-01

    The basis of inhibition of photosynthesis by single acute O{sub 3} exposures was investigated in vivo using analyses based on leaf gas exchange measurements. The fully expanded second leaves of wheat plants (Triticum aestivum L. cv Avalon) were fumigated with either 200 or 400 nanomoles per mole O{sub 3} for between 4 and 16 hours. This reduced significantly the light-saturated rate of Co{sub 2} uptake and was accompanied by a parallel decrease in stomatal conductance. However, the stomatal limitation only increased significantly during the first 8 hours of exposure to 400 nanomoles per mole O{sub 3}; no significant increase occurred for any of the other treatments. Analysis of the response of CO{sub 2} uptake to the internal Co{sub 2} concentration implied that the predominant factor responsible for the reduction in light-saturated CO{sub 2} uptake was a decrease in the efficiency of carboxylation. At saturating concentrations of Co{sub 2}, photosynthesis was inhibited by no more than 22% after 16 hours, indicating that the capacity for regeneration of ribulose bisphosphate was less susceptible to O{sub 3}. Ozone fumigations also had a less pronounced effect on light-limited photosynthesis. The photochemical efficiency of photosystem II estimated from the ratio of variable to maximum chlorophyll fluorescence and the atrazine-binding capacity of isolated thylakoids demonstrated that photochemical reactions were not responsible for the initial inhibition of CO{sub 2} uptake.

  7. Evidence of Recombinant Citrus tristeza virus Isolate Occurring in Acid Lime cv. Pant Lemon Orchard in Uttarakhand Terai Region of Northern Himalaya in India.

    PubMed

    Singh, Jaywant Kumar; Tarafdar, Avijit; Sharma, Susheel Kumar; Biswas, Kajal Kumar

    2013-06-01

    The present study for the first time describes biological and molecular characterization of Citrus tristeza virus (CTV) occurring in the Terai area of Uttarakhand State in Northern Himalaya region of India. Direct antigen coated-ELISA and reverse transcriptase-polymerase chain reaction (RT-PCR) detected the CTV infection in Acid lime cv. Pant lemon (Citrus aurantifolia) orchards of Pantnagar with an estimated disease incidence of 16.6-20.5 %. To know the biological and genetic properties, an isolate, CTV Pant 4 was characterized. Isolate Pant 4 could be graft transmitted to Kinnow, Nagpur and Darjeeling mandarins, Mosambi sweet orange, Kagzi lime, Sweet lime, Sour orange but not to Rough lemon. The sequence analyses of the 5'ORF1a (3038 nucleotides) of LPro domain and 3'end (2058 nt) covering ORF7-ORF10 regions of the CTV genome revealed that Pant 4 was closely related to the previously reported Indian CTV isolate, Kpg3 from Northeastern Himalaya region with 97 and 98 % sequence identity, respectively. Whereas, it differed from the previously reported CTV isolate B165 from Southern India with 79 and 92 % identity, respectively for 5'ORF1a and 3' end regions. Recombination and SplitsTree decomposition analyses indicated that CTV isolate Pant 4 was a recombinant isolate originating from Kpg3 as a major and B165 as a minor donor. PMID:24426255

  8. Characterization and sugar-binding properties of arcelin-1, an insecticidal lectin-like protein isolated from kidney bean (Phaseolus vulgaris L. cv. RAZ-2) seeds.

    PubMed Central

    Fabre, C; Causse, H; Mourey, L; Koninkx, J; Rivière, M; Hendriks, H; Puzo, G; Samama, J P; Rougé, P

    1998-01-01

    Arcelin-1 is a lectin-like protein found in the seeds of wild varieties of the kidney bean (Phaseolus vulgaris). This protein displays insecticidal properties, but the mechanism of action is as yet unknown. In the present study we investigated the biochemical and biophysical properties of arcelin-1 from Phaseolus vulgaris cv. RAZ-2. Native arcelin-1 is a dimeric glycoprotein of 60 kDa, built from the non-covalent association of two identical monomers. This dimer resists dissociation by chaotropic agents and is highly resistant to proteolytic enzymes. Each subunit contains 10% (w/w) neutral sugars which belong to the high-mannose and complex-type glycans attached to three glycosylation sites. No interaction of the protein with simple sugars could be detected, but arcelin-1 displays an intrinsic specificity in binding complex glycans. Arcelin-1 therefore differs from the closely related phytohaemagglutinin lectins and alpha-amylase inhibitor in several respects: oligomerization states, sugar-binding affinities and the type and number of glycan chains. These features may be related to the toxicity of arcelin-1. PMID:9445382

  9. Simultaneous treatment to attain blood pressure and lipid goals and reduced CV risk burden using amlodipine/atorvastatin single-pill therapy in treated hypertensive participants in a randomized controlled trial.

    PubMed

    Grimm, Richard; Malik, Mobin; Yunis, Carla; Sutradhar, Santosh; Kursun, Attila

    2010-01-01

    TOGETHER investigated whether targeting multiple cardiovascular (CV) risk factors using single-pill amlodipine/atorvastatin (AML/ATO) and therapeutic lifestyle changes (TLC) results in greater blood pressure (BP)/lipid control and additional reduction in estimated cardiovascular disease (CVD) risk compared with blood pressure intervention only using amlodipine (AML) + TLC. TOGETHER was a 6-week, randomized, double-blind, double-dummy trial using hypertensive participants with additional CV risk factors without CVD/diabetes. Participants were randomized to either AML/ATO (5 to 10/20 mg) + TLC or AML (5 to 10 mg) + TLC. The primary end point was the difference in proportion of participants attaining both BP (<140/90 mm Hg) and low-density lipoprotein cholesterol (LDL-C) (<100 mg/dL) goals at week 6. At week 6, 67.8% of participants receiving AML/ATO + TLC attained the combined BP/LDL-C goal versus 9.6% with AML + TLC (RD [A-B]: 58.2; 95% CI [48.1 to 68.4] P < 0.001; OR: 19.0; 95% CI 9.1 to 39.6; P < 0.001). Significant reductions from baseline in LDL-C, total cholesterol and triglycerides and estimated 10-year Framingham risk were also observed. Treatment with AML/ATO was well tolerated. In conclusion, a multifactorial CV management approach is more effective in achieving combined BP/LDL-C targets as well as CV risk reduction compared with BP intervention only in this patient population. PMID:20479948

  10. Simultaneous treatment to attain blood pressure and lipid goals and reduced CV risk burden using amlodipine/atorvastatin single-pill therapy in treated hypertensive participants in a randomized controlled trial

    PubMed Central

    Grimm, Richard; Malik, Mobin; Yunis, Carla; Sutradhar, Santosh; Kursun, Attila

    2010-01-01

    TOGETHER investigated whether targeting multiple cardiovascular (CV) risk factors using single-pill amlodipine/atorvastatin (AML/ATO) and therapeutic lifestyle changes (TLC) results in greater blood pressure (BP)/lipid control and additional reduction in estimated cardiovascular disease (CVD) risk compared with blood pressure intervention only using amlodipine (AML) + TLC. TOGETHER was a 6-week, randomized, double-blind, double-dummy trial using hypertensive participants with additional CV risk factors without CVD/diabetes. Participants were randomized to either AML/ATO (5 to 10/20 mg) + TLC or AML (5 to 10 mg) + TLC. The primary end point was the difference in proportion of participants attaining both BP (<140/90 mm Hg) and low-density lipoprotein cholesterol (LDL-C) (<100 mg/dL) goals at week 6. At week 6, 67.8% of participants receiving AML/ATO + TLC attained the combined BP/LDL-C goal versus 9.6% with AML + TLC (RD [A–B]: 58.2; 95% CI [48.1 to 68.4] P < 0.001; OR: 19.0; 95% CI 9.1 to 39.6; P < 0.001). Significant reductions from baseline in LDL-C, total cholesterol and triglycerides and estimated 10-year Framingham risk were also observed. Treatment with AML/ATO was well tolerated. In conclusion, a multifactorial CV management approach is more effective in achieving combined BP/LDL-C targets as well as CV risk reduction compared with BP intervention only in this patient population. PMID:20479948

  11. A bench-scale, cost effective and simple method to elicit Lycopersicon esculentum cv. PKM1 (tomato) plants against Cucumber mosaic virus attack using ozone-mediated inactivated Cucumber mosaic virus inoculum

    Microsoft Academic Search

    N. Sudhakar; D. Nagendra-Prasad; N. Mohan; K. Murugesan

    2007-01-01

    Studies were undertaken to evaluate ozone for inactivation of Cucumber mosaic virus present in the inoculum and to stimulate Lycopersicon esculentum cv. PKM1 (tomato) plants against Cucumber mosaic virus infection by using the inactivated Cucumber mosaic virus inoculum. Application of a T4 (0.4mg\\/l) concentration of ozone to the inoculum containing Cucumber mosaic virus resulted in complete inactivation of the virus.

  12. EFFECTS OF SINGLE, DUAL AND TRIPLE INOCULATIONS WITH BACILLUS SUBTILIS, BACILLUS MEGATERIUM AND RHIZOBIUM LEGUMINOSARUM BV. PHASEOLI ON NODULATION, NUTRIENT UPTAKE, YIELD AND YIELD PARAMETERS OF COMMON BEAN (PHASEOLUS VULGARIS L. CV. ‘ELKOCA-05’)

    Microsoft Academic Search

    Erdal Elkoca; Metin Turan; M. Figen Donmez

    2010-01-01

    This study was conducted in order to investigate the effects of single, dual, and triple inoculations with Rhizobium, N2-fixing Bacillus subtilis (OSU-142), and P-solubilizing Bacillus megaterium (M-3) on nodulation, plant growth, nutrient uptake and seed yield of common bean (Phaseolus vulgaris L. cv. ‘Elkoca-05’) in comparison to control and mineral fertilizer application under field conditions in 2006 and 2007 in

  13. [Studies on the performance of the dental air turbine handpieces. (Part 4) Analysis of the rotational performance of air turbine handpieces (N = N0 - CvFv gamma (author's transl)].

    PubMed

    Miyairi, H; Nagai, M; Fukuda, H; Muramatsu, A

    1981-04-01

    By the introduction of the diamond instruments and carbide burs measurably improved the quality and ease of cavity preparation, the several kinds of the air turbine handpieces, that is, standard type, torque type and miniature type air handpieces have been recently developed. In this paper, the performance tests of these air turbine handpieces have been carried out, and obtained the relations are indicated speeds and the vertical and horizontal loads or torque using the test bur. These relations are indicated by the logarithmic expressions, N = N0 - CvFv gamma, where N is the rotational speed of the air turbine handpieces during rotation, N0 is the free rotational speed of the air turbine handpieces, Fv is the vertical loads on the tests burs and Cv and gamma is the experimental constants. And then, the maximum performed work or the air turbine handpieces is presented by the expression as follows. W max = pimicrod/60(1/Cv)1/ gamma gamma (N0/gamma + 1)(1 + gamma)/gamma And we proposed the formula to be calculated for the performance ability of the air turbine handpieces. PMID:7026701

  14. Tecnologa para todos Sistema Inalmbrico de Rice

    E-print Network

    Tecnología para todos Sistema Inalámbrico de Rice Andrea Trevino Rice University http://www.techforall.org/tfa_wireless.html http://tfa.rice.edu #12;Por qué en Pecan Park? La Misión de TFA: Proporcionar conocimientos a la comunidad. El propósito de Rice: Usar este programa para explorar la siguiente generación del sistema

  15. Esta Preparado? Gua para los Tejanos en

    E-print Network

    ¿Esta Preparado? Guía para los Tejanos en caso de un Huracan Informacion de evacuacion Información://www.texasonline.com/portal/tol/es/emergency/specialneeds. Para Mas Informacion Oficina del Servicio Nacional de Meteorólogos - http://www.weather.gov/ os acompañan. Los tres son ciclones - sistemas organizados del clima que se desarrollan en los trópicos. Cada

  16. ¿APRENDO TRABAJANDO O APRENDER PARA TRABAJAR?

    Microsoft Academic Search

    Zahira Ojeda Bello; Misalys Hernández Pérez

    2009-01-01

    Los jóvenes cubanos, a diferencia de lo que viven muchos jóvenes en el mundo tienen garantizado el empleo y una legislación que los protege. Una vez graduados deben cumplir su servicio social y además se prevé una etapa de adiestramiento laboral, para lograr la adaptación y capacitación como trabajador en una entidad. Teniendo en cuenta la importancia que tiene para

  17. PROCEDIMIENTO para reservar espacios en Difusin

    E-print Network

    Islas, León

    MANUAL DE PROCEDIMIENTO para reservar espacios en Difusión Departamento de Difusión FACULTAD DE Departamento de Difusión, ubicado en la planta baja del edificio G. Acto seguido se verificarán los espacios y procedimiento para reservar espacios de difusión 2. El original de la Solicitud de Sala de Actos y Auditorio

  18. Ciudades inteligentes: beneficios para la inclusin social

    E-print Network

    Escolano, Francisco

    Ciudades inteligentes: beneficios para la inclusión social http://www.iuii.ua.es/CatedraTelefonicaUA/smart-cities Universidad de Alicante Inscripción gratuita a través del formulario http://www.iuii.ua.es/smart-cities trasladar los conceptos de Smart City al ámbito de la Universidad para lograr el objetivo de mejora de la

  19. Taller de Ciencia para Jvenes para alumnos de bachillerato San Cristobal de las Casas, Chiapas

    E-print Network

    Bor, Gil

    Taller de Ciencia para Jóvenes para alumnos de bachillerato San Cristobal de las Casas, Chiapas 3-7 agosto, 2009 CONVOCATORIA El "Taller de Ciencia para Jóvenes" en San Cristobal de las Casas (Chiapas), está dirigido a los alumnos de escuelas de nivel bachillerato que se sienten atraidos a las ciencias

  20. Estudio Exploratorio de los Factores para el Desarrollo de Sistemas de Informacion para Dispositivos Moviles

    Microsoft Academic Search

    Juan Manuel Gomez Reynoso; Juan Francisco Lozano Quezada; Monica del Refugio Brizuela Sandoval; Estela Lizbeth Munox Andrade; Carlos Argelio Arevalo Mercado

    2009-01-01

    Las limitaciones de los dispositivos móviles en cuanto a hardware, software, tamaño, entre otras, implican poner atención especial en el diseño de sistemas de información (SI) para los mismos. Las empresas necesitan reaccionar con rapidez al ambiente comercial tan cambiante, para no ser desplazadas en el mercado. La tecnología móvil está teniendo un gran auge para que las empresas respondan

  1. Becas de carcter general para el curso acadmico 2014-2015, para estudiantes que

    E-print Network

    Escolano, Francisco

    todos los estudios (estar en alguno de los siguientes casos): Requisitos Académicos (los créditosBecas de carácter general para el curso académico 2014-2015, para estudiantes que cursen estudios superior nivel al correspondiente al de los estudios para los que se solicita la beca. Ser español o tener

  2. Semana Nacional de Preparacin para Clima Severo 2014 Pgina 1 Plantilla para artculos de blog

    E-print Network

    auspiciada por la Agencia Federal para el Manejo de Emergencias (FEMA, por sus siglas en inglés) y la. ¿Qué puede hacer parar prepararse? Conocer su riesgo, tomar acción, y servir como ejemplo a la puede tomar para estar mejor preparado para salvar su vida y la de otros. Conozca su riesgo: Ya sean

  3. The Catabolism of (±)-Abscisic Acid by Excised Leaves of Hordeum vulgare L. cv Dyan and Its Modification by Chemical and Environmental Factors 1

    PubMed Central

    Cowan, A. Keith; Railton, Ian D.

    1987-01-01

    Excised light-grown leaves and etiolated leaves of Hordeum vulgare L. cv Dyan catabolized applied (±)-[2-14C]abscisic acid ([±]-[2-14C]ABA) to phaseic acid (PA), dihydrophaseic acid (DPA), and 2?-hydroxymethyl ABA (2?-HMABA). Identification of these catabolites was made by microchemical methods and by combined capillary gas chromatographymass spectrometry (GC-MS) following high dose feeds of nonlabeled substrate to leaves. Circular dichroism analysis revealed that 2?-HMABA was derived from the (?) enantiomer of ABA. By selecting tissue samples in which endogenous catabolites were undetectable by gas chromatography, it was possible to identify unequivocally ABA catabolites by GC-MS without the need to employ deuteriated substrate to distinguish the (±)-ABA catabolites from the same endogenous compounds. Refeeding studies were used to confirm the catabolic route. The methyl ester of (±)-[214C]-ABA was hydrolyzed efficiently by light-grown leaves of H. vulgare. Leaf age played a significant role in (±)-ABA catabolism, with younger leaves being less able than their older counterparts to catabolize this compound. The catabolism of (±)-ABA was inhibited markedly in water-stressed Hordeum leaves which was characterized by a decreased incorporation of label into 2?-HMABA, DPA, and conjugates. The specific, mixed function oxidase inhibitor, ancymidol, did not inhibit, dramatically, (±)-ABA catabolism in light-grown leaves of Hordeum whereas the 80s ribosome, translational inhibitor, cycloheximide, inhibited this process markedly. The 70s ribosome translational inhibitors, lincomycin and chloramphenicol, were less effective than cycloheximide in inhibiting (±)-ABA catabolism, implying that cytoplasmic protein synthesis is necessary for the catabolism of (±)-ABA in Hordeum leaves whereas chloroplast protein synthesis plays only a minor role. This further suggests that the enzymes involved in (±)-ABA catabolism in this plant are cytoplasmically synthesized and are `turned-over' rapidly, although the enzyme responsible for glycosylating (±)-ABA itself appeared to be stable. PMID:16665391

  4. A Novel Late Embryogenesis Abundant Like Protein Associated with Chilling Stress in Nicotiana tabacum cv. Bright Yellow-2 Cell Suspension Culture*

    PubMed Central

    Gai, Ying-Ping; Ji, Xian-Ling; Lu, Wei; Han, Xue-Juan; Yang, Guo-Dong; Zheng, Cheng-Chao

    2011-01-01

    Low temperature is one of the major abiotic stresses limiting the productivity and geographical distribution of many important crops. To identify proteins associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 (BY-2) cell suspension culture, we utilized a proteomic approach with two-dimensional electrophoresis to compare proteins from samples of treated with or without chilling treatment at 4 °C. One protein specifically more abundant in chilling treated sample was identified and designated as NtLEA7-3. Rapid amplification of cDNA ends gave rise to a full-length NtLEA7-3 cDNA with a complete open reading frame of 1267 bp, encoding a 322 amino acid polypeptide. Homology search and sequence multi-alignment demonstrated that the deduced NtLEA7-3 protein sequence shared a high identity with LEA-like proteins from other plants. Subcellular localization analysis indicated that the NtLEA7-3 was localized exclusively in the nucleus. When the gene was overexpressed in bright yellow-2 cells, the transgenic bright yellow-2 cells show more resistant to chilling stress than the wild-type cells. In addition, transgenic Arabidopsis plants overexpressing the NtLEA7-3 are much more resistant to cold, drought, and salt stresses. Interestingly, the expression of NtLEA7-3 in tobacco was not tissue-specific and induced by chilling, drought and salt stresses. All of these, taken together, suggest that NtLEA7-3 is worthwhile to elucidate the contribution of the proteins to the tolerance mechanism to chilling stress, and can be considered as a potential target for crop genetic improvement in the future. PMID:21653253

  5. Curdlan ?-1,3-Glucooligosaccharides Induce the Defense Responses against Phytophthora infestans Infection of Potato (Solanum tuberosum L. cv. McCain G1) Leaf Cells

    PubMed Central

    Li, Jing; Zhu, Li; Lu, Guangxing; Zhan, Xiao-Bei; Lin, Chi-Chung; Zheng, Zhi-Yong

    2014-01-01

    Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H2O2 and salicylic acid and the activities of phenylalanine amino-lyase, ?-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually. PMID:24816730

  6. Rare Earth Element Transfer from Soil to Navel Orange Pulp (Citrus sinensis Osbeck cv. Newhall) and the Effects on Internal Fruit Quality

    PubMed Central

    Cheng, Jinjin; Ding, Changfeng; Li, Xiaogang; Zhang, Taolin; Wang, Xingxiang

    2015-01-01

    The effects of soil rare earth element (REE) on navel orange quality and safety in rare earth ore areas have gained great attention. This study investigated the transfer characteristics of REE from soil to navel orange pulp (Citrus sinensis Osbeck cv. Newhall) and examined the effects of soil REE on internal fruit quality in Xinfeng County, Jiangxi province, China. Path analysis showed that soil REE, pH, cation exchange capacity (CEC), and Fe oxide (Feox) significantly affected pulp REE concentrations. A Freundlich-type prediction model for pulp REE was established: log[REEpulp] = -1.036 + 0.272 log[REEsoil] - 0.056 pH - 0.360 log[CEC] + 0.370 log[Feox] (n = 114, R2 = 0.60). From the prediction model, it was inferred that even when soil REE and Feox were as high as 1038 mg kg-1 and 96.4 g kg-1, respectively, and pH and CEC were as low as 3.75 and 5.08 cmol kg-1, respectively, pulp REE concentrations were much lower than the food limit standard. Additionally, soil REE levels were significantly correlated with selected fruit quality indicators, including titratable acidity (r = 0.52, P < 0.01), total soluble solids (r = 0.48, P < 0.01) and vitamin C (r = 0.56, P < 0.01). Generally, under routine methods of water and fertilization management, the cultivation of navel oranges in rare earth ore areas of south China with soil REE ranging from 38.6 to 546 mg kg-1 had improved in internal fruit quality. PMID:25806821

  7. Desiccation sensitivity and cryopreservation of excised embryonic axes of Citrus suhuiensis cv. limau madu, Citrumelo [Citrus paradisi macf. × Poncirus trifoliata (l.) raf.] and Fortunella polyandra.

    PubMed

    Al Zoubi, O M; Normah, M N

    2012-01-01

    Excised embryonic axes from seeds of three taxa, namely, Citrus suhuiensis cv. limau madu, Citrumelo (Citrus paradisi x Poncirus trifoliate) and Fortunella polyandra, were desiccated in a laminar airflow, over silica gel, and ultra-rapidly. Desiccation sensitivity (WC50) was estimated for each taxon using the quantal response model. High desiccation tolerance (WC50 = 0.11 g water per g dry mass. g/gdw) was observed for limau madu embryonic axes desiccated in a laminar airflow and ultra-rapidly (WC50 =0.10 g/gdw). Desiccation tolerance was substantially lower (WC50 = 0.19 g/gdw) for silica gel dehydration. Similarly, high desiccation tolerance (WC50 = 0.15 g/gdw) was associated with F. polyandra embryonic axes when desiccated in a laminar airflow, while a lower desiccation tolerance (WC50 = 0.17 g/gdw) was observed with silica gel dehydration. Ultra-rapid desiccation led to the highest desiccation tolerance (WC50 = 0.14 g/gdw). The dehydration rate, however, had no influence on desiccation tolerance (WC50 ~ 0.14 g/gdw) for Citrumelo embryonic axes. After each desiccation period, embryonic axes were directly immersed in liquid nitrogen (LN) followed by rapid rewarming. Normal seedling recovery of 80 to 83% for excised embryonic axes of limau madu was observed for laminar airflow and ultra-rapid dehydration, but for silica gel dehydration, 57% recovery was obtained. Similarly, for Citrumelo, high recoveries of 100% and 97% were obtained from axes desiccated in a laminar airflow and using ultra-rapid dehydration, respectively, whereas a lower value was associated with silica gel dehydration (80%). For F. polyandra, 50% recovery was obtained both for laminar airflow and ultra-rapid dehydration, while much lower recovery (43%) was associated with silica gel dehydration. Regardless of the drying method employed, axis survival percentages following exposure to LN were commensurate with the desiccation sensitivity pattern. PMID:22825791

  8. Microbial activity and water-soluble trace element species in the rhizosphere of spring wheat (Triticum aestivum cv. USU-Perigee).

    PubMed

    Turmel, Marie-Claude; Courchesne, François; Cloutier-Hurteau, Benoît

    2011-04-01

    The influence of microbial activity on the concentration and speciation of trace elements (TEs) was assessed in a study on the bioavailability of TEs for edible plants. A growth chamber experiment with spring wheat (Triticum aestivum cv. USU-Perigee) was conducted and the bulk (Bk) and the rhizosphere (Rz) soil components were collected at maturity. A characterization of the microbial activity and population was made by measuring the microbial biomass, enzymes (acid phosphatase, arylsulfatase, dehydrogenase and urease) and 16S rDNA DGGE profiles. In soil water extracts, major solutes (H(+), Ca, Mg, Na, NH(4), K, Cl, NO(3), SO(4), total N, DON and DOC) and trace elements (Al, As, Cd, Ce, Cr, Cu, Fe, Pb, Tl, and Zn) including monomeric Al species, free Cu(2+) and labile Zn were determined. The partition of the variation indicated that 12.1% of the distribution of TEs in the Bk soil was significantly and exclusively explained by chemical properties while this value was less than 0.1% for the Rz soil. To the contrary, microbial properties contributed significantly to 12.3% of the distribution of TEs in the Rz soil whereas it explained less than 0.1% for the Bk soil. Detailed redundancy analyses identified several potential mechanisms (e.g. weathering of primary mineral, solubilisation of sesquioxides, bacterial effect on the redox status) explaining the fate of TEs in the Bk and Rz soils. This study revealed that microbial activity is strongly associated to the speciation of trace elements in the Rz of edible plants and points to some microbial processes influencing TE speciation. PMID:21359293

  9. Curdlan ?-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells.

    PubMed

    Li, Jing; Zhu, Li; Lu, Guangxing; Zhan, Xiao-Bei; Lin, Chi-Chung; Zheng, Zhi-Yong

    2014-01-01

    Activation of the innate immune system before the invasion of pathogens is a promising way to improve the resistance of plant against infection while reducing the use of agricultural chemicals. Although several elicitors were used to induce the resistance of potato plant to microbial pathogen infection, the role of curdlan oligosaccharide (CurdO) has not been established. In the current study, the defense responses were investigated at biochemical and proteomic levels to elucidate the elicitation effect of CurdOs in foliar tissues of potato (Solanum tuberosum L. cv. McCain G1). The results indicate that the CurdOs exhibit activation effect on the early- and late-defense responses in potato leaves. In addition, glucopentaose was proved to be the shortest active curdlan molecule based on the accumulation of H?O? and salicylic acid and the activities of phenylalanine amino-lyase, ?-1,3-glucanase and chitinase. The 2D-PAGE analysis reveals that CurdOs activate the integrated response reactions in potato cells, as a number of proteins with various functions are up-regulated including disease/defense, metabolism, transcription, and cell structure. The pathogenesis assay shows that the ratio of lesion area of potato leaf decreased from 15.82%±5.44% to 7.79%±3.03% when the plants were treated with CurdOs 1 day before the infection of Phytophthora infestans. Furthermore, the results on potato yield and induction reactions indicate that the defense responses induced by CurdOs lasted for short period of time but disappeared gradually. PMID:24816730

  10. Pre-exposure of calli to ozone promotes tolerance of regenerated Lycopersicon esculentum cv. PKM1 plantlets against acute ozone stress.

    PubMed

    Nagendra-Prasad, D; Sudhakar, N; Murugesan, K; Mohan, N

    2008-08-25

    Studies were performed to evaluate the effects of pre-exposure of calli to ozone in promoting tolerance of the regenerated Lycopersicon esculentum cv. PKM1 (tomato) plantlets against acute ozone stress (AOS). Calli induced from tomato leaf explants were subjected to pre-treatment with ozone: T(1)=100 ppb, T(2)=200 ppb and T(3)=300 ppb. For the control (C) calli, charcoal-filtered air was supplied to test differential sensitivity of regenerated plantlets to acute ozone stress. All treated calli were subsequently transferred to shooting, rooting medium and acclimatized. The plantlets regenerated from the respective ozone (T(1), T(2), T(3))-treated calli are referred to here as T(1), T(2), T(3) plantlets and the plantlets regenerated from control calli are referred to as control plantlets. The frequencies of regeneration of tomato plantlets from the calli were T(1)=86%, T(2)=82% and T(3)=67%, and 92% regeneration was obtained from control calli. In order to evaluate the ozone tolerance, all the regenerated plantlets were exposed to the acute ozone exposure (AOE). After AOE, the T(2) plantlets endured remarkably well by experiencing reduced ozone stress, which was evident from the lower level of hydrogen peroxide and oxidative stress-related enzymes such as ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1.15.1.1) activities relative to T(3), T(1) and C plantlets. All T(2) plantlets showed enhanced tolerance against AOE by upholding enhanced soluble phenol content, a higher level of foliar and apoplastic ascorbic acid, elevated dehydroascorbate reductase (EC 1.8.5.1) and glutathione content. The present study reveals that the calli pre-exposed to T(2) ozone treatment resulted in an increase in the level of antioxidants and provided the plants greater protection against acute ozone stress. PMID:18160125

  11. Catabolism of (+/-)-abscisic acid by excised leaves of Hordeum vulgare L. cv Dyan and its modification by chemical and environmental factors

    SciTech Connect

    Cowan, A.K.; Railton, I.D.

    1987-05-01

    Excised light-grown leaves and etiolated leaves of Hordeum vulgare L. cv Dyan catabolized applied (+/-)-(2-/sup 14/C)abscisic acid ((+/-)-(2-/sup 14/C)ABA) to phaseic acid (PA), dihydrophaseic acid (DPA), and 2'-hydroxymethyl ABA (2'-HMABA). Identification of these catabolites was made by microchemical methods and by combined capillary gas chromatography-mass spectrometry (GC-MS) following high dose feeds of nonlabeled substrate to leaves. Circular dichroism analysis revealed that 2'-HMABA was derived from the (-) enantiomer of ABA. Refeeding studies were used to confirm the catabolic route. The methyl ester of (+/-)-(2/sup 14/C)-ABA was hydrolyzed efficiently by light-grown leaves of H. vulgare. Leaf age played a significant role in (+/-)-ABA catabolism, with younger leaves being less able than their older counterparts to catabolize this compound. The catabolism of (+/-)-ABA was inhibited markedly in water-stressed Hordeum leaves which was characterized by a decreased incorporation of label into 2'-HMABA, DPA, and conjugates. The specific, mixed function oxidase inhibitor, ancymidol, did not inhibit, dramatically (+/-)-ABA catabolism in light-grown leaves of Hordeum whereas the 80s ribosome, translational inhibitor, cycloheximide, inhibited this process markedly. The 70s ribosome translational inhibitors, lincomycin and chloramphenicol, were less effective than cycloheximide in inhibiting (+/-)-ABA catabolism, implying that cytoplasmic protein synthesis is necessary for the catabolism of (+/-)-ABA in Hordeum leaves whereas chloroplast protein synthesis plays only a minor role. This further suggests that the enzymes involved in (+/-)-ABA catabolism in this plant are cytoplasmically synthesized and are turned-over rapidly, although the enzyme responsible for glycosylating (+/-)-ABA itself appeared to be stable.

  12. Influence of fermentation conditions on glucosinolates, ascorbigen, and ascorbic acid content in white cabbage (Brassica oleracea var. capitata cv. Taler) cultivated in different seasons.

    PubMed

    Martinez-Villaluenga, C; Peñas, E; Frias, J; Ciska, E; Honke, J; Piskula, M K; Kozlowska, H; Vidal-Valverde, C

    2009-01-01

    The content of glucosinolates (GLS), ascorbigen, and ascorbic acid in white cabbage (Brassica oleracea var. capitata cv. Taler) cultivated in different seasons (summer and winter) was determined, before and after spontaneous and starter-induced fermentation. Different salt concentrations (0.5% NaCl or 1.5% NaCl) were used for sauerkraut production. Glucoiberin, sinigrin, and glucobrassicin were dominating in raw white cabbage cultivated either in winter or summer seasons. Ascorbigen precursor, glucobrassicin, was found higher in cabbage cultivated in winter (2.54 micromol/g dw) than those grown in summer (1.83 micromol/g dw). Cabbage fermented for 7 d was found to contain only traces of some GLS irrespective of the fermentation conditions used. Ascorbigen synthesis occurred during white cabbage fermentation. Brining cabbage at low salt concentration (0.5% NaCl) improved ascorbigen content in sauerkraut after 7 d of fermentation at 25 degrees C. The highest ascorbigen concentration was observed in low-sodium (0.5% NaCl) sauerkraut produced from cabbage cultivated in winter submitted to either natural (109.0 micromol/100 g dw) or starter-induced fermentation (108.3 and 104.6 micromol/100 g dw in cabbages fermented by L. plantarum and L. mesenteroides, respectively). Ascorbic acid content was found higher in cabbage cultivated in summer and fermentation process led to significant reductions. Therefore, the selection of cabbages with high glucobrassicin content and the production of low-sodium sauerkrauts may provide enhanced health benefits towards prevention of chronic diseases. PMID:19200088

  13. The influence of crop management on banana weevil, Cosmopolites sordidus (Coleoptera: Curculionidae) populations and yield of highland cooking banana (cv. Atwalira) in Uganda.

    PubMed

    Rukazambuga, N D T M; Gold, C S; Gowen, S R; Ragama, P

    2002-10-01

    A field study was undertaken in Uganda using highland cooking banana (cv. Atwalira) to test the hypothesis that bananas grown under stressed conditions are more susceptible to attack by Cosmopolites sordidus (Germar). Four banana treatments were employed to create different levels of host-plant vitality: (1) high stress: intercrop with finger millet; (2) moderate stress: monoculture without soil amendments; (3) low stress: monoculture with manure; (4) high vigour: monoculture with continuous mulch and manure. Adult C. sordidus were released at the base of banana mats 11 months after planting and populations were monitored for three years using mark and recapture methods. Cosmopolites sordidus density was greatest in the mulched plots which may have reflected increased longevity and/or longer tenure time in moist soils. Lowest C. sordidus numbers were found in intercropped banana. Damage, estimated as percentage corm tissue consumed by larvae, was similar among treatments. However, the total amount of tissue consumed was greater in mulched banana than in other systems. Plants supporting the heaviest levels of C. sordidus damage displayed bunch size reductions of 40-55%. Banana yield losses ranged from 14-20% per plot with similar levels in the intercropped and mulched systems. Yield reductions, reported as t ha-1, were twice as high in the mulched system as in the intercrop. The results from this study indicate that C. sordidus problems are not confined to stressed banana systems or those with low levels of management, but that the weevil can also attain pest status in well-managed and productive banana stands. PMID:12241566

  14. Improved Maturity and Ripeness Classifications of Magnifera Indica cv. Harumanis Mangoes through Sensor Fusion of an Electronic Nose and Acoustic Sensor

    PubMed Central

    Zakaria, Ammar; Shakaff, Ali Yeon Md; Masnan, Maz Jamilah; Saad, Fathinul Syahir Ahmad; Adom, Abdul Hamid; Ahmad, Mohd Noor; Jaafar, Mahmad Nor; Abdullah, Abu Hassan; Kamarudin, Latifah Munirah

    2012-01-01

    In recent years, there have been a number of reported studies on the use of non-destructive techniques to evaluate and determine mango maturity and ripeness levels. However, most of these reported works were conducted using single-modality sensing systems, either using an electronic nose, acoustics or other non-destructive measurements. This paper presents the work on the classification of mangoes (Magnifera Indica cv. Harumanis) maturity and ripeness levels using fusion of the data of an electronic nose and an acoustic sensor. Three groups of samples each from two different harvesting times (week 7 and week 8) were evaluated by the e-nose and then followed by the acoustic sensor. Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were able to discriminate the mango harvested at week 7 and week 8 based solely on the aroma and volatile gases released from the mangoes. However, when six different groups of different maturity and ripeness levels were combined in one classification analysis, both PCA and LDA were unable to discriminate the age difference of the Harumanis mangoes. Instead of six different groups, only four were observed using the LDA, while PCA showed only two distinct groups. By applying a low level data fusion technique on the e-nose and acoustic data, the classification for maturity and ripeness levels using LDA was improved. However, no significant improvement was observed using PCA with data fusion technique. Further work using a hybrid LDA-Competitive Learning Neural Network was performed to validate the fusion technique and classify the samples. It was found that the LDA-CLNN was also improved significantly when data fusion was applied. PMID:22778629

  15. Sequence-Based SSR Marker Development and Their Application in Defining the Introgressions of LA0716 (Solanum pennellii) in the Background of cv. M82 (Solanum lycopersicum)

    PubMed Central

    Long, Wenbo; Li, Ye; Zhou, Wenjuan; Ling, Hong-Qing; Zheng, Shusong

    2013-01-01

    The introgression lines (ILs) from cv. M82 (Solanum lycopersicum) × LA0716 (S. pennellii) have been proven to be exceptionally useful for genetic analysis and gene cloning. The introgressions were originally defined by RFLP markers at their development. The objectives of this study are to develop polymorphic SSR markers, and to re-define the DNA introgression from LA0716 in the ILs. Tomato sequence data was scanned by software to generate SSR markers. In total, 829 SSRs, which could be robustly amplified by PCR, were developed. Among them, 658 SSRs were dinucleotide repeats, 162 were trinucleotide repeats, and nine were tetranucleotide repeats. The 829 SSRs together with 96 published RFLPs were integrated into the physical linkage map of S. lycopersicum. Introgressions of DNA fragments from LA0716 were re-defined among the 75 ILs using the newly developed SSRs. A specific introgression of DNA fragment from LA0716 was identified in 72 ILs as described previously by RFLP, whereas the specific DNA introgression described previously were not detected in the ILs LA4035, LA4059 and LA4091. The physical location of each investigated DNA introgression was finely determined by SSR mapping. Among the 72 ILs, eight ILs showed a shorter and three ILs (IL3-2, IL12-3 and IL12-3-1) revealed a longer DNA introgression than that framed by RFLPs. Furthermore, 54 previously undefined segments were found in 21 ILs, ranging from 1 to 11 DNA introgressions per IL. Generally, the newly developed SSRs provide additional markers for genetic studies of tomatoes, and the fine definition of DNA introgressions from LA0716 would facilitate the use of the ILs for genetic analysis and gene cloning. PMID:24339899

  16. Salicylic Acid Alleviates the Adverse Effects of Salt Stress in Torreya grandis cv. Merrillii Seedlings by Activating Photosynthesis and Enhancing Antioxidant Systems

    PubMed Central

    Du, Xuhua; Tang, Hui; Shen, Chaohua; Wu, Jiasheng

    2014-01-01

    Background Salt stress is a major factor limiting plant growth and productivity. Salicylic acid (SA) has been shown to ameliorate the adverse effects of environmental stress on plants. To investigate the protective role of SA in ameliorating salt stress on Torreya grandis (T. grandis) trees, a pot experiment was conducted to analyze the biomass, relative water content (RWC), chlorophyll content, net photosynthesis (Pn), gas exchange parameters, relative leakage conductivity (REC), malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD) and peroxidase (POD) of T. grandis under 0.2% and 0.4% NaCl conditions with and without SA. Methodology/Principal Findings The exposure of T. grandis seedlings to salt conditions resulted in reduced growth rates, which were associated with decreases in RWC and Pn and increases in REC and MDA content. The foliar application of SA effectively increased the chlorophyll (chl (a+b)) content, RWC, net CO2 assimilation rates (Pn), and proline content, enhanced the activities of SOD, CAT and POD, and minimized the increases in the REC and MDA content. These changes increased the capacity of T. grandis in acclimating to salt stress and thus increased the shoot and root dry matter. However, when the plants were under 0% and 0.2% NaCl stress, the dry mass of the shoots and roots did not differ significantly between SA-treated plants and control plants. Conclusions SA induced the salt tolerance and increased the biomass of T. grandis cv. by enhancing the chlorophyll content and activity of antioxidative enzymes, activating the photosynthetic process, and alleviating membrane injury. A better understanding about the effect of salt stress in T. grandis is vital, in order gain knowledge over expanding the plantations to various regions and also for the recovery of T. grandis species in the future. PMID:25302987

  17. Significant changes in cell and chloroplast development in young wheat leaves (Triticum aestivum cv Hereward) grown in elevated CO{sub 2}

    SciTech Connect

    Robertson, E.J.; Leech, R.M. [Univ. of York, Heslington (United Kingdom)

    1995-01-01

    Cell and chloroplast development were characterized in young Triticum aestivum cv Hereward leaves grown at ambient (350 {mu}L L{sup {minus}1}) or at elevated (650 {mu}L L{sup {minus}1}) CO{sub 2}. In elevated CO{sub 2}, cell and chloroplast expansion was accelerated by 10 and 25%, respectively, in the first leaf of 7-d-old wheat plants without disruption to the leaf developmental pattern. Elevated CO{sub 2} did not affect the number of chloroplasts in relation to mesophyll cell size or the linear relationship between chloroplast number or size and mesophyll cell size. No major changes in leaf anatomy or in chloroplast ultrastructure were detected as a result of growth in elevated CO{sub 2}, but there was a marked reduction in starch accumulation. In leaf sections fluorescently tagged antisera were used to visualize and quantitate the amount of cytochrome f, the {alpha}- and {beta}-subunits of the coupling factor 1 in ATP synthase, D1 protein of the photosystem II reaction center, the 33-kD protein of the extrinsic oxygen-evolving complex, subunit II of photosystem I, and ribulose-1,5-biphosphate carboxylase/oxygenase. A significant finding was that in 10 to 20% of the mesophyll cells grown in elevated CO{sub 2} the 33-kD protein of the extrinsic oxygen-evolving complex of photosystem II and cytochrome f were deficient by 75%, but the other proteins accumulated normally. 29 refs., 6 figs., 2 tabs.

  18. Extracellular compounds produced by fungi associated with botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells.

    PubMed

    Ramírez-Suero, M; Bénard-Gellon, M; Chong, J; Laloue, H; Stempien, E; Abou-Mansour, E; Fontaine, F; Larignon, P; Mazet-Kieffer, F; Farine, S; Bertsch, C

    2014-11-01

    Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. One hypothesis is that the extracellular compounds produced by the fungi associated with grapevine trunk diseases are responsible for pathogenicity.In the present work, we used Vitis vinifera cv. Chardonnay cells to test the aggressiveness of total extracellular compounds produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with botryosphaeria dieback. Additionally, the toxicity of purified mellein, a characteristic toxin present in the extracellular compounds of Botryosphaeriaceae, was assessed.Our results show that the total extracellular compounds produced by N. parvum induce more necrosis on Chardonnay calli and induce a different defence gene expression pattern than those of D. seriata. Mellein was produced by both fungi in amounts proportional to its aggressiveness. However, when purified mellein was added to the culture medium of calli, only a delayed necrosis and a lower-level expression of defence genes were observed. Extracellular compounds seem to be involved in the pathogenicity of the fungi associated with botryosphaeria dieback. However, the doses of mellein used in this study are 100 times higher than those found in the liquid fungal cultures: therefore, the possible function of this toxin is discussed. PMID:24752796

  19. Curriculum vitae (CV) Susan Murcott

    E-print Network

    Reuter, Martin

    -cities in newly industrializing countries, § Impact: Reached 8+ million people in China and Brazil. o Environmental health and sanitation ­ solid, liquid, toxic chemical and waste management § Impact: created the first compendium of arsenic contamination in water/food in the world, identifying site-specific arsenic

  20. Nadia L. Zakamska CV Information

    E-print Network

    Weaver, Harold A. "Hal"

    , funded) Herschel (Sept 2011, funded) Gemini (GMOS, March 2010) Gemini (NIFS, March 2010) APO 3.5m (Triplespec, spring 2009, summer 2009) Gemini (GMOS, March 2006) Chandra (March 2005, funded) Spitzer (Nov proposals: Gemini (GMOS, Sept 2012) Spitzer warm mission Spies survey (legacy proposal, funded, Aug 2012