Sample records for cv africana para

  1. Avaliação da cv. Cabernet Sauvignon para elaboração de vinho tinto

    Microsoft Academic Search

    Luiz Antenor Rizzon; Alberto Miele

    2002-01-01

    SUMMARY EVALUATION OF THE CV. CABERNET SAUVIGNON IN THE MANUFACTURE OF RED WINE. The Cabernet Sauvignon grape was developed in Bordeaux, France. It was introduced in Brazil in 1921, but its cultivation in the viticultural region of Serra Gaúcha only grew since 1980. It is used in the manufacture of aged or young red wines. Due to its importance, this

  2. AVALIAÇÃO DA CV. MERLOT PARA ELABORAÇÃO DE VINHO TINTO 1

    Microsoft Academic Search

    Luiz Antenor RIZZON; Alberto MIELE

    2004-01-01

    -1 de acidez titulável; e pH de 3,49. Sob o aspecto sensorial, o vinho Merlot apresenta cor vermelho-violáceo com boa intensidade. Gustativamente distingue-se pela maciez, equilíbrio e fineza. Palavras-chave: enologia; uva; Vitis vinifera; vinho; caracterização. SUMMARY EVALUATION OF CV. MERLOT TO MAKE RED WINE - Merlot is a traditional grape of the Saint Émillion wines, Bordeaux region - France. It

  3. Department of Africana Studies

    E-print Network

    Sorin, Eric J.

    332 Civil Rights and the Law; AFRS 354 African American Women and the Civil Rights Movement Take and Chair California State University, Long Beach 1250 Bellflower Blvd., Long Beach, CA 90840-0905 (562) 985;Bachelor of Arts in Africana Studies Major Requirements A minimum of 39 units is required for the major

  4. Africana.com: The Digital Bridge

    NSDL National Science Digital Library

    Africana.com was founded in 1999 to present "information and commentary reflecting the diverse concerns of people of African descent." Designed by Harvard Square Netcasting, with Harvard University professors Henry Louis Gates and Anthony Appiah on the its Board of Directors, Africana.com provides a broad spectrum of information from scholarship to lifestyle articles and headline news. Several features boost the interactivity of the site: a free email service for registrants, a quiz (AfriQuest), and Radio Africana, listings of radio stations worldwide webcasting Black music. There are also links to related Web resources, a media center (primarily books and CDs for purchase), and the Adopt-A-School philanthropy program.

  5. 126 SDSU General Catalog 2011-2012 Africana Studies

    E-print Network

    Gallo, Linda C.

    Offered by the Department Major in Africana studies with the B.A. degree in liberal arts and sciences, consulting, librarianship, counseling, program development, program design, program analysis, urbanology requirement. Africana Studies Major With the B.A. Degree in Liberal Arts and Sciences (Major Code: 22111

  6. Epidemiological aspects of Claviceps africana, causal agent of Sorghum ergot

    E-print Network

    Noe, Montes Garcia; Noe, Montes Garcia

    2005-02-17

    Sorghum ergot, caused by Claviceps africana Frederickson, Mantle & de Milliano, is a disease that affects non-fertilized ovaries in sorghum male-sterile plants and infects hybrids if there is pollen sterility at flowering time. Sphacelia containing...

  7. Studies on the genus Setaria Viborg, 1795 in South Africa. I. Setaria africana (Yeh, 1959).

    PubMed

    Watermeyer, R; Boomker, J; Putterill, J F

    2000-12-01

    Setaria africana (Yeh, 1959) is represented by two subspecies, Setaria africana africana Troncy, Graber & Thal, 1976 from giant eland (Taurotragus derbianus) from the Central African Republic and Cameroon and Setaria africana farchai Troncy, Graber & Thal, 1976 from bushbuck (Tragelaphus scriptus), also from the Central African Republic. Material collected from nyala (Tragelaphus angasii), bushbuck and kudu (Tragelaphus strepsiceros) from several localities in the eastern region of South Africa was re-examined. Measurements of adult worms confirmed the differences between the two subspecies and scanning electron microscopy showed that the deirids of S. africana africana are single whereas those of S. africana farchaiare double. Setaria africana farchaiis recorded for the first time in South Africa. PMID:11206389

  8. 126 SDSU General Catalog 2013-2014 Africana Studies

    E-print Network

    Gallo, Linda C.

    and sciences. Minor in Africana studies. Minor in African studies. See Interdisciplinary Programs. The Major, research, consulting, librarianship, counseling, program development, program design, program analysis Arts and Sciences (Major Code: 22111) (SIMS Code: 110301) All candidates for the degree in liberal arts

  9. 126 SDSU General Catalog 2012-2013 Africana Studies

    E-print Network

    Gallo, Linda C.

    and sciences. Minor in Africana studies. Minor in African studies. See Interdisciplinary Programs. The Major, research, consulting, librarianship, counseling, program development, program design, program analysis Arts and Sciences (Major Code: 22111) (SIMS Code: 110301) All candidates for the degree in liberal arts

  10. 122 SDSU General Catalog 2010-2011 Africana Studies

    E-print Network

    Gallo, Linda C.

    and sciences. Minor in Africana studies. Minor in African studies. See Interdisciplinary Programs. The Major, research, consulting, librarianship, counseling, program development, program design, program analysis Arts and Sciences (Major Code: 22111) (SIMS Code: 110301) All candidates for the degree in liberal arts

  11. Extraction and utilization of breadfruit seed oil ( Treculia africana)

    Microsoft Academic Search

    V. I. E. Ajiwe; C. A. Okeke; H. U. Agbo

    1995-01-01

    Oil was extracted from crushed breadfruit (Treculia africana) seeds by Soxhlet. The yield of the oil was 20·83 ± 0·57%. Tests showed the oil to be a semi-drying oil which is unsaturated, with a high saponification value, acidic and requires purification. The oil could be used for making soap, hair shampoo and alkyd resin.

  12. Dominance rank relationships among wild female African elephants, Loxodonta africana

    E-print Network

    Alberts, Susan C

    Dominance rank relationships among wild female African elephants, Loxodonta africana ELIZABETH A of Biology, Duke University yTarangire Elephant Project, Arusha, Tanzania zAmboseli Elephant Research Project, like African savanna elephants, are predicted to have unresolved dominance hierarchies and poorly

  13. Fermentation of Agave tequilana juice by Kloeckera africana: influence of amino-acid supplementations.

    PubMed

    Valle-Rodríguez, Juan Octavio; Hernández-Cortés, Guillermo; Córdova, Jesús; Estarrón-Espinosa, Mirna; Díaz-Montaño, Dulce María

    2012-02-01

    This study aimed to improve the fermentation efficiency of Kloeckera africana K1, in tequila fermentations. We investigated organic and inorganic nitrogen source requirements in continuous K. africana fermentations fed with Agave tequilana juice. The addition of a mixture of 20 amino-acids greatly improved the fermentation efficiency of this yeast, increasing the consumption of reducing sugars and production of ethanol, compared with fermentations supplemented with ammonium sulfate. The preference of K. africana for each of the 20 amino-acids was further determined in batch fermentations and we found that asparagine supplementation increased K. africana biomass production, reducing sugar consumption and ethanol production (by 30, 36.7 and 45%, respectively) over fermentations supplemented with ammonium sulfate. Therefore, asparagine appears to overcome K. africana nutritional limitation in Agave juice. Surprisingly, K. africana produced a high concentration of ethanol. This contrasts to poor ethanol productivities reported for other non-Saccharomyces yeasts indicating a relatively high ethanol tolerance for the K. africana K1 strain. Kloeckera spp. strains are known to synthesize a wide variety of volatile compounds and we have shown that amino-acid supplements influenced the synthesis by K. africana of important metabolites involved in the bouquet of tequila. The findings of this study have revealed important nutritional limitations of non-Saccharomyces yeasts fermenting Agave tequilana juice, and have highlighted the potential of K. africana in tequila production processes. PMID:21761236

  14. Neuronal morphology in the African elephant ( Loxodonta africana ) neocortex

    Microsoft Academic Search

    Bob Jacobs; Jessica Lubs; Markus Hannan; Kaeley Anderson; Camilla Butti; Chet C. Sherwood; Patrick R. Hof; Paul R. Manger

    2011-01-01

    Virtually nothing is known about the morphology of cortical neurons in the elephant. To this end, the current study provides\\u000a the first documentation of neuronal morphology in frontal and occipital regions of the African elephant (Loxodonta africana). Cortical tissue from the perfusion-fixed brains of two free-ranging African elephants was stained with a modified Golgi\\u000a technique. Neurons of different types (N = 75),

  15. Prevalence of Candida albicans-closely related yeasts, Candida africana and Candida dubliniensis, in vulvovaginal candidiasis.

    PubMed

    Shan, Yingying; Fan, Shangrong; Liu, Xiaoping; Li, Jianling

    2014-08-01

    Isolates of Candida africana and C. dubliniensis were recovered from patients with vulvovaginal candidiasis (VVC). The isolates were initially identified as C. albicans through use of the API Candida System. We retrospectively reexamined 1014 vaginal isolates presumptively determined to be C. albicans at the Department of Obstetrics and Gynecology of Peking University Shenzhen Hospital from 1 January 2003 through 31 December 2012. Our objective was to determine, via detection of the HWP1 gene, if any of the isolates were C. africana or C. dubliniensis. One and a half percent of these isolates (15/1014) were found to be C. africana, whereas C. dubliniensis was not detected. The 15 C. africana isolates were susceptible to nystatin, fluconazole, itraconazole, miconazole, and clotrimazole. Candida africana could not be recovered from clinical vaginal specimens from the 15 patients at follow-up on days 7-14 and days 30-35 when treated with different antifungal agents. We conclude that C. africana, but not C. dubliniensis, was present in the vaginal samples of patients with VVC. The C. africana isolates were susceptible to the tested antifungal agents. VVC caused by C. africana appears to respond well to current therapies. PMID:25023482

  16. Molecular Epidemiology of Candida albicans and Its Closely Related Yeasts Candida dubliniensis and Candida africana?

    PubMed Central

    Romeo, Orazio; Criseo, Giuseppe

    2009-01-01

    We performed a molecular study to determine the occurrence of Candida albicans, Candida africana, and Candida dubliniensis in different clinical samples. The study provides new insights into the epidemiology of candidiasis in hospitalized patients in three hospitals in southern Italy. It also reports the first detailed epidemiological data concerning the occurrence of C. africana in clinical samples. PMID:18987171

  17. Real-time PCR detection of sorghum ergot pathogens Claviceps africana, C. sorghi, and C. sorghicola

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sorghum ergot is a serious disease that has caused major losses in sorghum growing regions worldwide. Claviceps africana is now the most widely distributed species causing ergot in many countries including the U.S., whereas both C. africana and C. sorghi exist in India. A third species (C. sorghic...

  18. Real-time PCR detection of sorghum ergot pathogens Claviceps africana, C. sorghi, and C. sorghicola

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sorghum ergot is a serious disease that has caused major losses in sorghum growing regions worldwide. C. africana is now the most widely distributed species causing ergot in many countries including the U.S., whereas both C. africana and C. sorghi exist in India. A third species (C. sorghicola) ha...

  19. A review of historical trends in the distribution and abundance of elephants Loxodonta africana

    E-print Network

    Pretoria, University of

    A review of historical trends in the distribution and abundance of elephants Loxodonta africana in Mozambique C.P. Ntumi, S.M. Ferreira and R.J. van Aarde Abstract The elephant Loxodonta africana population that the impact of human activity on the elephant population in- creased after the onset of the colonial era

  20. Rural Africana; Current Research in the Social Sciences. Rural Education, Fall 1969.

    ERIC Educational Resources Information Center

    Sheffield, James R., Ed.; And Others

    The bulletin is one in the series "Rural Africana" published 3 times yearly by the African Studies Center, Michigan State University. "Rural Africana" is devoted to current research in the social sciences, exploring the problems of social and economic development in rural Africa south of the Sahara. The present issue is a compilation of papers…

  1. CV Surgery Online

    NSDL National Science Digital Library

    Stanford University's HighWire Press has announced the online publication of several journals. CV Surgery Online offers "a fully searchable online compilation of articles pertinent to the cardiothoracic and vascular surgeon from the 5 American Heart Association Journals: Circulation, Circulation Research, Hypertension, Stroke, and Arteriosclerosis, Thrombosis, and Vascular Surgery." Published by the American Heart Association in conjunction with HighWire Press, the free trial period for CV Surgery Online ended December 31, 2000.

  2. Uncommon mandibular osteomyelitis in a cat caused by Nocardia africana

    PubMed Central

    2012-01-01

    Background Nocardiosis is an unusual infection in companion animals characterized by suppurative to pyogranulomatous lesions, localized or disseminated. Cutaneous-subcutaneous, pulmonary and systemic signs are observed in feline nocardiosis. However, osteomyelitis is a rare clinical manifestation in cats. Nocardia cyriacigeorgica (formerly N. asteroides sensu stricto), Nocardia brasiliensis, Nocardia otitidiscaviarum, and Nocardia nova are the most common pathogenic species identified in cats, based on recent molecular classification (16S rRNA gene). The present report is, to our knowledge, the first case of mandibular osteomyelitis in a cat caused by Nocardia africana, diagnosed based upon a combination of methods, including molecular techniques. Case presentation A one-year-old non-neutered female cat, raised in a rural area, was admitted to the Companion Animal Hospital-PUCPR, São José dos Pinhais, State of Paraná, Brazil, with a history a progressive facial lesion, difficulty apprehending food, loss of appetite, apathy and emaciation. Clinical examination showed fever, submandibular lymphadenitis, and a painless, 8 cm diameter mass, which was irregularly-shaped, of firm consistency, and located in the region of the left mandible. The skin around the lesion was friable, with diffuse inflammation (cellulitis), multiple draining sinuses, and exudation of serosanguinous material containing whitish “sulfur” granules. Diagnosis was based initially in clinical signs, microbiological culture, cytological, and histopathological findings, and radiographic images. Molecular sequencing of 16S rRNA of isolate allowed diagnosis of Nocardia africana. Despite supportive care and antimicrobial therapy based on in vitro susceptibility testing the animal died. Conclusion The present report describes a rare clinical case of feline osteomyelitis caused by Nocardia africana, diagnosed based upon a combination of clinical signs, microbiological culture, cytological and histopathological findings, radiographic images, and molecular methods. The use of modern molecular techniques constitutes a quick and reliable method for Nocardia species identification, and may contribute to identification to new species of Nocardia that are virulent in cats. PMID:23216681

  3. MASTER: CV, DNe, variables.

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Gress, O.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Antipin, S.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Chazov, V.; Vladimirov, V.; Kuvshinov, D.; Shurpakov, S.; Shumkov, V.; Potter, S.; Kniazev, A.; Kotze, M.; Gress, O.; Ivanov, K.; Budnev, N.; Yazev, S.; Poleshchuk, V.; Konstantinov, E.; Chuvalaev, O.; Tlatov, A.; Dormidontov, D.; Senik, V.; Parkhomenko, A.; Yurkov, V.; Sergienko, Y.; Gabovich, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.

    2015-03-01

    MASTER OT J105529.06-255654.5 discovery - new CV MASTER-SAAO auto-detection system ( Lipunov et al., Advances in Astronomy, MASTER Global Robotic Net, 2010 ) discovered OT source at (RA, Dec) = 10h 55m 29.06s -25d 56m 54.5s on 2015-03-11.89942 UT.

  4. Sorghum Ergot: Distinguishing Sphacelia and Sclerotia of Claviceps africana in Seed

    E-print Network

    Frederickson, Debra; Odvody, Gary; Isakeit, Thomas

    1999-07-05

    . Sclerotia are ?resting? or dormancy structures equipped to withstand degradation by the L-5315 6-99 Sorghum Ergot Distinguishing Sphacelia and Sclerotia of Claviceps africana in Seed Debra E. Frederickson and Gary N. Odvody Assisted by Thomas Isakeit...

  5. Morphological, biochemical and molecular characterisation of the first Italian Candida africana isolate.

    PubMed

    Romeo, Orazio; Criseo, Giuseppe

    2009-09-01

    One atypical isolate of the pathogenic yeast Candida albicans was isolated from an Italian patient with vulvovaginitis. The strain, germ tube positive and chlamydospore-negative showed white-thin turquoise colonies on Candida ID 2 medium. The yeast was identified as Candida africana by using morphological and biochemical tests. On the basis of the molecular results obtained in this study as well as in other studies, C. africana cannot be yet considered as a new species of Candida. It is possible that C. africana represents a new variant of C. albicans like the well-known Candida stellatoidea. To our knowledge, this is the first isolation of C. africana in Italy. PMID:18983430

  6. Why Do African Elephants (Loxodonta africana) Simulate Oestrus? An Analysis of Longitudinal Data

    E-print Network

    Why Do African Elephants (Loxodonta africana) Simulate Oestrus? An Analysis of Longitudinal Data, United Kingdom, 3 Amboseli Trust for Elephants, Nairobi, Kenya Abstract Female African elephants signal that parous females in the Amboseli elephant population do simulate receptive oestrus behaviours

  7. Gut microflora of two saltmarsh detritivore thalassinid prawns, Upogebia africana and Callianassa kraussi

    Microsoft Academic Search

    Jean M. Harris; Lindsay J. Seiderer; Michael I. Lucas

    1991-01-01

    The presence and digestive capabilities of bacteria associated with the digestive systems and habitats of two saltmarsh-burrowing\\u000a detritivore thalassinid prawns (Upogebia africana andCallianassa kraussi) was examined.U. africana is a filter-feeding prawn inhabiting muddy deposits, whereasC. kraussi, a deposit feeder, inhabits coarser more sandy deposits. Scanning electron microscopy was used to examine the gut lining\\u000a and associated microflora and the nature

  8. Epidemiology, antifungal susceptibility, and pathogenicity of Candida africana isolates from the United Kingdom.

    PubMed

    Borman, Andrew M; Szekely, Adrien; Linton, Chistopher J; Palmer, Michael D; Brown, Phillipa; Johnson, Elizabeth M

    2013-03-01

    Candida africana was previously proposed as a new species within the Candida albicans species complex, together with C. albicans and C. dubliniensis, although further phylogenetic analyses better support its status as an unusual variant within C. albicans. Here we show that C. africana can be distinguished from C. albicans and C. dubliniensis by pyrosequencing of a short region of ITS2, and we have evaluated its occurrence in clinical samples by pyrosequencing all presumptive isolates of C. albicans submitted to the Mycology Reference Laboratory over a 9-month period. The C. albicans complex constituted 826/1,839 (44.9%) of yeast isolates received over the study period and included 783 isolates of C. albicans, 28 isolates of C. dubliniensis, and 15 isolates of C. africana. In agreement with previous reports, C. africana was isolated exclusively from genital specimens, in women in the 18-to-35-year age group. Indeed, C. africana constituted 15/251 (6%) of "C. albicans" isolates from female genital specimens during the study period. C. africana isolates were germ tube positive, grew significantly more slowly than C. albicans and C. dubliniensis on conventional mycological media, could be distinguished from the other members of the C. albicans complex by appearance on chromogenic agar, and were incapable of forming chlamydospores. Here we present the detailed evaluation of epidemiological, phenotypic, and clinical features and antifungal susceptibility profiles of United Kingdom isolates of C. africana. Furthermore, we demonstrate that C. africana is significantly less pathogenic than C. albicans and C. dubliniensis in the Galleria mellonella insect systemic infection model. PMID:23303503

  9. Reproductive strategies of the kangaroo leech, Marsupiobdella africana (Glossiphoniidae)

    PubMed Central

    Kruger, Natasha; Du Preez, Louis

    2015-01-01

    The Kangaroo Leech, Marsupiobdella africana, is a hermaphroditic organism, with insemination taking place by the planting of a spermatophore on another leech. Spermatophores are mostly planted on the anterior of the recipient leech, but not always. Several spermatophores may be planted by different leeches on a single recipient. The spermatophore consists of two side by side lobes. Within minutes from planting of the spermatophore, the contents are squeezed out and into the body of the recipient. Sperm are believed to find the way to the ova by following chemical cues. Kangaroo Leeches display advanced parental care by transferring fertilized eggs from the reproductive opening to a brood pouch on the ventral side. Fully developed leeches may copulate after detaching from the amphibian host Xenopus laevis, or from the Cape River Crab Potamonautes perlatus with which it maintains a phoretic association. PMID:25830114

  10. Bilateral ovarian cystadenoma in a geriatric African elephant (Loxodonta africana).

    PubMed

    Hoby, Stefan; Aloisio, Fabio; Schumacher, Vanessa L

    2014-06-01

    A 59-yr-old, captive female African elephant (Loxodonta africana) died of a cardiovascular collapse. Necropsy revealed bilateral replacement of the ovarian tissue by multiple cystic formations of up to 10 cm in diameter. The cysts were thin walled and filled with clear watery fluid. Smaller solid masses with an irregular surface projected from the wall of a few of the cysts. Histologically, the cystic structures were characterized by a single layer of well-differentiated cuboidal epithelial cells resting on a basement membrane. Occasionally the cysts contained nodular proliferations of single-layered, well-differentiated cuboidal epithelial cells forming anastomosing tubules and occasional papillary projections. The ovarian neoplasia was diagnosed as a bilateral multilocular serous ovarian cystadenoma. The nulliparous status and the advanced age may have contributed to the ovarian pathology. PMID:25000708

  11. Exploratory rigid laparoscopy in an African elephant (Loxodonta africana).

    PubMed

    Sweet, Julia; Hendrickson, Dean A; Stetter, Mark; Neiffer, Donald L

    2014-12-01

    In March 2009, a 25-yr-old captive female African elephant (Loxodonta africana) underwent an exploratory laparoscopy after several weeks of diarrhea, submandibular and ventral edema, and swelling on medial and lateral aspects of all feet. Although there have been recent advances in laparoscopic vasectomies in free-ranging African elephants in South Africa utilizing specially designed rigid laparoscopes and insufflation devices, this was the first attempt at using these same techniques for an exploratory purpose. The elephant was sedated in a static restraint chute and remained standing for the duration of the procedure. Laparoscopy provided visibility of the dorsal abdomen, enabled collection of reproductive tract biopsies and peritoneal fluid samples, and allowed for instillation of antibiotics and crystalloid fluids directly into the abdominal cavity. Abdominal exploration, collection of tissue samples, and local therapy is possible via standing laparoscopy in megavertebrates. PMID:25632688

  12. Reproductive strategies of the kangaroo leech, Marsupiobdella africana (Glossiphoniidae).

    PubMed

    Kruger, Natasha; Du Preez, Louis

    2015-04-01

    The Kangaroo Leech, Marsupiobdella africana, is a hermaphroditic organism, with insemination taking place by the planting of a spermatophore on another leech. Spermatophores are mostly planted on the anterior of the recipient leech, but not always. Several spermatophores may be planted by different leeches on a single recipient. The spermatophore consists of two side by side lobes. Within minutes from planting of the spermatophore, the contents are squeezed out and into the body of the recipient. Sperm are believed to find the way to the ova by following chemical cues. Kangaroo Leeches display advanced parental care by transferring fertilized eggs from the reproductive opening to a brood pouch on the ventral side. Fully developed leeches may copulate after detaching from the amphibian host Xenopus laevis, or from the Cape River Crab Potamonautes perlatus with which it maintains a phoretic association. PMID:25830114

  13. Cartilha para Legalizao de Casas Religiosas

    E-print Network

    Cartilha para Legalização de Casas Religiosas de Matriz Africana #12;2 Rio de Janeiro, 30 de maio Secretaria Estadual de Assistência Social e Direitos Humanos do Rio de Janeiro. NIREMA: Núcleo Social e Direitos Humanos do Rio de Janeiro Coordenador Geral Prof. Adriano Pilatti Centro de Ciências

  14. Segmentação, Indexação e Recuperação de Vídeo Utilizando OpenCV

    Microsoft Academic Search

    Thiago Teixeira Santos; Carlos Hitoshi Morimoto

    Resumo: Os constantes avanços em poder de processamento, capacidade de armazenamento, e largura de banda tornaram possível ao usuário de com- putadores o acesso, manipulação e edição de imagens e vídeos digitais. Nesse tutorial, vamos descrever alguns algoritmos de visão computacional para a seg- mentação, indexação e recuperação de vídeo utilizando o OpenCV para que, ao final do tutorial, você

  15. CV-990 LSRA

    NASA Technical Reports Server (NTRS)

    1992-01-01

    A NASA CV-990, modified as a Landing Systems Research Aircraft (LSRA), is serviced on the ramp at NASA's Dryden Flight Research Center, Edwards, California, before a test of the space shuttle landing gear system. The space shuttle landing gear test unit, operated by a high-pressure hydraulic system, allowed engineers to assess and document the performance of space shuttle main and nose landing gear systems, tires and wheel assemblies, plus braking and nose wheel steering performance. The series of 155 test missions for the space shuttle program provided extensive data about the life and endurance of the shuttle tire systems and helped raise the shuttle crosswind landing limits at Kennedy.

  16. "Criminally Unjust: Young People and the Crisis of Mass Incarceration" Conference presented by The Africana Criminal Justice Project and the Institute for Research in African-American Studies

    E-print Network

    Qian, Ning

    by The Africana Criminal Justice Project and the Institute for Research in African-American Studies Columbia University: My name is Laurent Alfred. I'm coordinator of the Africana Criminal Justice Project. I'd like, who's been a strong supporter of the Africana Criminal Justice Project and all of our endeavors

  17. CV 100--Still Going Strong

    ERIC Educational Resources Information Center

    Abrahams, A. L.

    1976-01-01

    Describes results of a study that used CV 100, a fuel additive for use in oil-fired heating systems, on a trial basis in 12 Ontario schools. The test showed an average 12 percent reduction in fuel costs in the schools using CV 100. (JG)

  18. Year Action IB # Jig HAL CP HAL CV Trawl CP Trawl CV

    E-print Network

    Year Action IB # Jig Pot Gear (CP and CV) HAL CP HAL CV Trawl CP Trawl CV TOTAL TAC ABC OFL 396 5,841 Year Action IB # Jig Pot Gear (CV and CP) HAL CP HAL CV 50 HAL CV ,408 NOTES: IB = Information Bulletin TAC = Total Allowable Catch ABC = Acceptable Biological Catch OFL

  19. Cultural and Agenda Conflicts in Academia: Critical Issues for Africana Women's Studies.

    ERIC Educational Resources Information Center

    Hudson-Weems, Clenora

    1989-01-01

    Suggests that women of African descent should name and define their unique movement "Africana Womanism" to distinguish it from limitations implicit in the notion of Black feminism. The movement advocates the rejection of all vestiges of White patriarchy and seeks to combat the triple oppressions of racism, classism, and sexism. (AF)

  20. W.E.B. Du Bois's Evolving Africana Philosophy Of Education

    Microsoft Academic Search

    Reiland Rabaka

    2003-01-01

    The aim of this article is to analyze W.E.B. Du Bois's educational thought for its key contributions to contemporary Africana philosophy of education. To succinctly explore Du Bois's philosophy of education, the article outlines some of the ways his pedagogical theories and practices are inextricable from, and virtually incomprehensible without critically considering, his philosophy of history, concepts of culture, economic

  1. W.E.B. Du Bois and the Encyclopedia Africana, 1909-63

    Microsoft Academic Search

    Henry Louis Gates

    2000-01-01

    In 1901, W.E.B. Du Bois, the African American intellectual and activist, conceived the idea of an encyclopedia Africana. He envisioned a scientific and comprehensive work on Africa and peoples of African descent that would refute the Enlightenment notion of blacks as devoid of civilization and the hallmarks of humanity. Du Bois stood in a tradition of earlier black works seeking

  2. CLASSIFICATION OF AFRICAN ELEPHANT LOXODONTA AFRICANA RUMBLES USING ACOUSTIC PARAMETERS AND CLUSTER ANALYSIS

    Microsoft Academic Search

    JASON D. WOOD; BRENDA MCCOWAN; WILLIAM R. LANGBAUER JR; JOZUA J. VILJOEN; LYNETTE A. HART

    2005-01-01

    It has been suggested that African savanna elephants Loxodonta africana produce 31 different call types (Langbauer 2000). Various researchers have described these calls by associating them with specific behavioural contexts. More recently Leong et al. (2003) have attempted to classify elephant call types based on their physical properties. They classified 8 acoustically distinct call types from a population of captive

  3. Performance of African breadfruit ( Treculia africana Decne) seed flour in bread making

    Microsoft Academic Search

    Sunday Y. Giami; Tamunosaki Amasisi

    2003-01-01

    Replacing wheat flour (WHF) with flour (ABF) from roasted African breadfruit (Treculia africana Decne) seeds at levels of 0-25% was investigated for its effect on bread making properties. Resistance to extension (R), extensibility (E) and mechanical work for deformation (W) of doughs prepared from the blends were studied using a Chopin alveograph. Loaves were prepared using the straight-dough bread baking

  4. Use of space and habitat by elephants (Loxodonta africana) in the Maputo

    E-print Network

    Pretoria, University of

    Use of space and habitat by elephants (Loxodonta africana) in the Maputo Elephant Reserve 9 September 2004. Accepted 2 July 2005 Satellite tracking units fitted to five elephants in the Maputo Elephant Reserve provided information on habitat use. We used the CALHOME program with Adaptive

  5. Hierarchical dominance structure and social organization in African elephants, Loxodonta africana

    E-print Network

    Getz, Wayne M.

    Hierarchical dominance structure and social organization in African elephants, Loxodonta africana GSave the Elephants, Nairobi, Kenya (Received 6 June 2006; initial acceptance 26 August 2006; final acceptance 12 and relative intensity of competition at these two levels. African elephants have well-structured social

  6. Rules of habitat use by elephants Loxodonta africana in southern Africa: insights for regional management

    E-print Network

    Pretoria, University of

    Rules of habitat use by elephants Loxodonta africana in southern Africa: insights for regional in southern Africa are concerned that continually increasing elephant populations will degrade ecosystems. Culling, translocation and birth control are flawed solutions. An alternative is providing elephants more

  7. Solutions for elephant Loxodonta africana crop raiding in northern Botswana: moving away from symptomatic approaches

    E-print Network

    Pretoria, University of

    Solutions for elephant Loxodonta africana crop raiding in northern Botswana: moving away from Conflict between people and elephants in Africa is widespread yet many solutions target the symptoms of the problem need to be examined. Here we examine factors underlying spatial use by elephants and people along

  8. Larval release rhythms and tidal exchange in the estuarine mudprawn, Upogebia africana

    Microsoft Academic Search

    T. H. Wooldridge; H. Loubser

    1996-01-01

    The mudprawn, Upogebia africana is common in intertidal regions of many South African estuaries. The life cycle is complex, incorporating a marine phase of development during the larval stages. Breeding peaks are in summer and first-stage larvae are released into the plankton at night. Maximum release activity and export to the marine environment follow a semi-lunar cycle synchronized to the

  9. The Only African Wild Tobacco, Nicotiana africana: Alkaloid Content and the Effect of Herbivory

    PubMed Central

    Marlin, Danica; Nicolson, Susan W.; Yusuf, Abdullahi A.; Stevenson, Philip C.; Heyman, Heino M.; Krüger, Kerstin

    2014-01-01

    Herbivory in some Nicotiana species is known to induce alkaloid production. This study examined herbivore-induced defenses in the nornicotine-rich African tobacco N. africana, the only Nicotiana species indigenous to Africa. We tested the predictions that: 1) N. africana will have high constitutive levels of leaf, flower and nectar alkaloids; 2) leaf herbivory by the African bollworm Helicoverpa armigera will induce increased alkaloid levels in leaves, flowers and nectar; and 3) increased alkaloid concentrations in herbivore-damaged plants will negatively affect larval growth. We grew N. africana in large pots in a greenhouse and exposed flowering plants to densities of one, three and six fourth-instar larvae of H. armigera, for four days. Leaves, flowers and nectar were analyzed for nicotine, nornicotine and anabasine. The principal leaf alkaloid was nornicotine (mean: 28 µg/g dry mass) followed by anabasine (4.9 µg/g) and nicotine (0.6 µg/g). Nornicotine was found in low quantities in the flowers, but no nicotine or anabasine were recorded. The nectar contained none of the alkaloids measured. Larval growth was reduced when leaves of flowering plants were exposed to six larvae. As predicted by the optimal defense theory, herbivory had a localized effect and caused an increase in nornicotine concentrations in both undamaged top leaves of herbivore damaged plants and herbivore damaged leaves exposed to one and three larvae. The nicotine concentration increased in damaged compared to undamaged middle leaves. The nornicotine concentration was lower in damaged leaves of plants exposed to six compared to three larvae, suggesting that N. africana rather invests in new growth as opposed to protecting older leaves under severe attack. The results indicate that the nornicotine-rich N. africana will be unattractive to herbivores and more so when damaged, but that potential pollinators will be unaffected because the nectar remains alkaloid-free even after herbivory. PMID:25025217

  10. Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC.

    PubMed Central

    Agyare, Christian; Dwobeng, Anita Serwaa; Agyepong, Nicholas; Boakye, Yaw Duah; Mensah, Kwesi Boadu; Ayande, Patrick George; Adarkwa-Yiadom, Martin

    2013-01-01

    Microbial infections of various types of wounds are a challenge to the treatment of wounds and wound healing. The study was to investigate antimicrobial and antioxidant properties of methanol leaf and stem bark extracts of Kigelia africana and methanol leaf and root extracts of Strophanthus hispidus and also to determine wound healing properties of the extracts. The antimicrobial activities of the methanol extracts were determined against two Gram-positive and two Gram-negative bacteria and a fungus using agar diffusion and micro-dilution methods. The antioxidant activity was determined using 1,1-diphenyl-2-picryl–hydrazyl (DPPH) method. The influence of the extracts on rate of wound closure was investigated using the excision wound model and histopathological investigation of treated and untreated wound tissues performed. The MICs of leaf extract of K. africana against test organisms were 2.5–7.5?mg/mL and stem bark extract were 2.25–7.5?mg/mL. The leaf extract of S. hispidus had MIC range of 2.5–7.5?mg/mL and 2.5–10?mg/mL for root extract. The IC50 of leaf and stem bark extracts of K. africana were 56.9 and 13.7??g/mL, respectively and leaf and root of S. hispidus were 49.8 and 45.1??g/mL, respectively. K. africana extracts (7.5% w/w) showed significant (P < 0.05) wound contraction at day 7 with 72% of wound closure whiles significant (P < 0.05) wound contractions were observed on day 11 for stem bark of K. africana, leaf and root extracts of S. hispidus. Wound tissues treated with the extracts showed improved collagenation, re-epitheliazition and rapid granulation formation compared with untreated wound tissues. The extracts were found to contain alkaloids, saponins, tannins, flavonoids, carbohydrates, and sapogenetic glycosides. The HPLC finger-printing of the extracts were developed. The leaf, stem bark and root extracts of K. africana and S. hispidus exhibited antimicrobial, antioxidant, and enhanced wound healing properties and these may justify the medicinal uses of the plants for treatment of microbial infections and wounds. PMID:23662099

  11. Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid ( Ansellia africana Lindl.)

    Microsoft Academic Search

    Rajeswari Vasudevan; Johannes Van Staden

    The effects of explant and cytokinin types on in vitro plant regeneration of Ansellia africana were investigated. The exogenous addition of cytokinins is not required for the proliferation of new protocorms from Trimmed\\u000a protocorm cluster (TPC) explants. To the contrary, nodal and shoot-tip explants produced a single shoot in response to the\\u000a addition of cytokinins. Overall plant growth in terms

  12. In vitro screening and evaluation of fungicides for the control of Claviceps africana on sorghum seed

    Microsoft Academic Search

    S. A. Bhuiyan; V. J. Galea; M. J. Ryley; D. Tay

    2000-01-01

    Eleven fungicides, benomyl, bitertanol, captan, carbendazim, mancozeb, procymidone, propiconazole, tebuconazole, thiram, triadimenol\\u000a and tridemorph, were tested in vitro at 50 and 10 mg a.i.\\/L for their effects on the macroconidial germination of Claviceps africana, the sorghum ergot pathogen. Thiram and captan completely inhibited conidial germination of the two isolates (UQE1 and UQE2)\\u000a tested. Nine of the best fungicides from this

  13. [Chemical and DNA analyses for the products of a psychoactive plant, Voacanga africana].

    PubMed

    Kikura-Hanajiri, Ruri; Maruyama, Takuro; Miyashita, Akinori; Goda, Yukihiro

    2009-08-01

    Voacanga africana (Apocynaceae) is a small tropical African tree. The root bark and seeds of this tree contain a number of alkaloids, including ibogaine (a hallucinogenic/aphrodisiac compound in bark), tabersonine (a major constituteent of seeds) and other voacanga alkaloids, traditionally used in Africa for religious purposes. Recently, some kinds of products containing this plant (root bark and seeds) have been distributed in the drug market in expectation of its hallucinogenic/aphrodisiac effects. There has been no report that has discussed quantitative analyses of these alkaloids in the products and their botanical origins. In this study, to investigate the trend of such a non-controlled psychotropic plant of abuse, a simultaneous analytical method was developed using LC/MS for the voacanga alkaloids including ibogaine and tabersonine in the commercial products of V. africana. Moreover, the botanical origins of these products were investigated by DNA analyses. As a result of the LC/MS analyses, the products were classified into two chemical types; an ibogaine-type and a tabersonine-type. The samples of the ibogaine-type contain ibogaine (0.05-0.6%) and other voacanga alkaloids; voacamine, voacamidine and voacangine, while those of the tabersonine-type mainly contain tabersonine (0.6-1.6%). The sequence analyses of chloroplast DNA, trnL-F region suggested that most of the products were derived from V. africana or closely related plants. They were classified into four genotypes based on nucleotide sequence of the trnL-F IGS region. The proposed methods of chemical and DNA analyses would be useful for investigating the trend in the distribution of the products of V. africana. PMID:19652504

  14. Master-Saao new CV

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Kornilov, V.; Kuznetsov, A.; Tiurina, N.; Chazov, V.; Potter, S.; Kniazev, A.; Kotze, M.; Gress, O.; Ivanov, K.; Budnev, N.; Yazev, S.; Konstantinov, E.; Chuvalaev, O.; Poleshchuk, V.; Tlatov, A.; Dormidontov, D.; Senik, V.; Parkhomenko, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Yurkov, V.; Sergienko, Y.; Gabovich, A.; Shumkov, V.; Shurpakov, S.

    2015-02-01

    MASTER OT J014522.24-280325.6 - new CV MASTER-SAAO auto-detection system ( Lipunov et al., MASTER Global Robotic Net, Advances in Astronomy, 2010 ) discovered OT source at (RA, Dec) = 01h 45m 22.24s -28d 03m 25.6s on 2015-02-13.80071 UT.

  15. Paratrichodina africana (Ciliophora: Trichodinidae) of wild and cultured Nile tilapia in the Northern Brazil.

    PubMed

    Tavares-Dias, Marcos; Marchiori, Natália da Costa; Martins, Maurício Laterça

    2013-01-01

    The present work morphologically characterizes Paratrichodina africana from the gills of wild and farmed Nile tilapia from Northern Brazil (eastern Amazonia). Ninety fish were captured for parasitological analysis in Macapá, State of Amapá, from a wetland area bathed by the Amazon River commonly called 'Ressaca do Zerão' (n = 52), as well as from a local fish farm (n = 38). Wet smears of the skin and gills of the captured fish were air dried at room temperature and impregnated with silver nitrate by Klein's method for posterior examination of the adhesive disc structures. Total prevalence of parasitism was 16.6% (23% in fish from wetland and 7.8% in farmed fish). Characterized as a small-sized trichodinid, it presented the following measures: 33.2 ± 4.7 µm body diameter, 17.5 ± 2.1 µm adhesive disc, 10.0 ± 0.9 µm denticulate ring, and 22.6 ± 2.0 denticles. Paratrichodina africana reported in this study strongly resembles those described for other localities, but it differs by presenting greater body length. This is the fourth report of P. africana parasitizing a host fish. PMID:23802238

  16. Open CV intro Aside: Histogram Equalization

    E-print Network

    Kosecka, Jana

    Open CV intro Aside: Histogram Equalization Images are from Wikipedia. #12;Today we'll code: A fully functional sparse optical flow algorithm! Plan ·! OpenCV Basics ­! What is it? ­! How do you get started with it? ·! Feature Finding and Optical Flow ­! A brief mathematical discussion. ·! OpenCV

  17. Introduction to OpenCV David Stavens

    E-print Network

    Thrun, Sebastian

    1 Introduction to OpenCV David Stavens Stanford Artificial Intelligence Lab Tonight we'll code extensively in the 223b competition.) Plan OpenCV Basics What is it? How do you get started with it? Feature Finding and Optical Flow A brief mathematical discussion. OpenCV Implementation of Optical Flow Step

  18. An Indigenous Plant Food Used by Lactating Mothers in West Africa: The Nutrient Composition of the Leaves of Kigelia Africana in Ghana

    Microsoft Academic Search

    R. S. Glew; B. Amoako-Atta; G. Ankar-Brewoo; J. M. Presley; Y.-C. Chang; L.-T. Chuang; M. Millson; B. R. Smith; R. H. Glew

    2010-01-01

    Although the leaves of Kigelia africana are used to make a palm-nut soup which is consumed mainly by lactating women in many parts of sub-Saharan Africa, little is known about the nutrient qualities of this underutilized and underappreciated plant food. Leaves of Kigelia africana, called “sausage tree” in English and “nufuten” in the Twi language of Ghana, were collected in

  19. Nutrient Composition of African Breadfruit (Treculia africana) Seed Hull and its Use in Diets for the African Giant Land Snail, Archachatina marginata

    Microsoft Academic Search

    2007-01-01

    A feeding trial was conducted to evaluate the use of Treculia africana seed hull meal (TASHM) as replacement for maize in diets for the African giant land snail, Archachatina marginata. Seeds of T. africana were parboiled, dehulled, milled and used to replace maize at 0%, 15%, 30% and 45% (diets I, II, III and IV, respectively) in isoproteic diets (20%

  20. CV

    E-print Network

    2014-08-14

    Aug 14, 2014 ... Rand, D.G., Zhou, Q., Buzzard, G.T., and Fox, J.J., Computationally efficient strategy for modeling ... doi: 10.1016/j.aca.2012.10.005. 36. Buzzard, G.T., Efficient basis ... Proceeding, O'Farrell Street, San Francisco, CA, 2011. 3.

  1. CV

    E-print Network

    2013-09-23

    My interests in biology, health, and medicine, combined with my fascination with ... research has been mathematical models in cancer and infectious diseases, ... Education. • Ph.D. in Mathematics, Vanderbilt University, Nashville, TN, 2012.

  2. CV

    E-print Network

    2015-03-31

    Mar 31, 2015 ... Rand, D.G., Zhou, Q., Buzzard, G.T., and Fox, J.J., Computationally efficient strategy for modeling ... doi: 10.1016/j.aca.2012.10.005. 36. Buzzard, G.T., Efficient basis ... Proceeding, O'Farrell Street, San Francisco, CA, 2011. 3.

  3. CV

    E-print Network

    2014-10-29

    October 2014. Current Position ... NSF Research Grant “Boundary Effects in Critical Phenomena” as PI ($267,705) ... 30th International Colloquium on Group Theoretical Methods .... Referee for “Journal of Physics A”,“Physics Letters A” and

  4. Two jacalin-related lectins from seeds of the African breadfruit (Treculia africana L.).

    PubMed

    Shimokawa, Michiko; Nsimba-Lubaki, Shadrack Makuta; Hayashi, Namiko; Minami, Yuji; Yagi, Fumio; Hiemori, Keiko; Tateno, Hiroaki; Hirabayashi, Jun

    2014-01-01

    Two jacalin-related lectins (JRLs) were purified by mannose-agarose and melibiose-agarose from seeds of Treculia africana. One is galactose-recognizing JRL (gJRL), named T. africana agglutinin-G (TAA-G), and another one is mannose-recognizing JRL (mJRL), TAA-M. The yields of the two lectins from the seed flour were approximately 7.0 mg/g for gJRL and 7.2 mg/g for mJRL. The primary structure of TAA-G was determined by protein sequencing of lysyl endopeptic peptides and chymotryptic peptides. The sequence identity of TAA-G to other gJRLs was around 70%. Two-residue insertion was found around the sugar-binding sites, compared with the sequences of other gJRLs. Crystallographic studies on other gJRLs have shown that the primary sugar-binding site of gJRLs can accommodate Gal, GalNAc, and GalNAc residue of T-antigen (Gal?1-3GalNAc?-). However, hemagglutination inhibition and glycan array showed that TAA-G did not recognize GalNAc itself and T-antigen. TAA-G preferred melibiose and core 3 O-glycan. PMID:25155899

  5. Speaking the invisible : Africana women, black identity, and alienation in the works of Nella Larsen and Tsitsi Dangarembga

    Microsoft Academic Search

    Regina L Bryant

    2003-01-01

    This study examines black identity and alienation Nella Larsen's and Tsitsi Dangarembgal’s Passing and Nervous Conditions. The novels demonstrate the authors' interpretation of the conditions within their respective societies of the impact of slavery and colonization on Africana women. As a springboard in the development of these issues, Frantz Fanon's seminal works Black Skin, White Masks and The Wretched of

  6. LABORATORY GREENHOUSE, AND FIELD ASSESSMENT OF FOURTEEN FUNGICIDES FOR ACTIVITY AGAINST CLAVICEPS AFRICANA, CAUSAL AGENT OF SORGHUM ERGOT

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Commericial formulations of 14 fungicides representing seven chemical classes were assessed in vitro and in vivo for activity against Claviceps africana, causal agent of sorghum ergot. All the fungicides markedly reduced spore germination in vitro on water agar, with EC50 values (based on active ing...

  7. Mortality trends in the African cherry ( Prunus africana) and the implications for colobus monkeys ( Colobus guereza) in Kakamega Forest, Kenya

    Microsoft Academic Search

    Peter J. Fashing

    2004-01-01

    Prunus africana (Hook.f.) Kalkm. is a secondary forest canopy tree species that has been declining over much of its geographical range in sub-Saharan Africa during recent decades due to unsustainable harvesting of its bark for the international medicinal plant trade. One of the locations where the species is experiencing rapid mortality is Isecheno study site in the Kakamega Forest, Kenya

  8. Effect of heat processing on in vitro protein digestibility and some chemical properties of African breadfruit ( Treculia africana Decne) seeds

    Microsoft Academic Search

    Sunday Y. Giami; Matthew N. Adindu; Alexandria D. Hart; Emmanuel O. Denenu

    2001-01-01

    The effects of dry heat (roasting) and moist heat (boiling) on in vitro protein digestibility, protein fractions and other chemical properties of African breadfruit (Treculia africana Decne) seed that affect their utilization as a source of human food were investigated. Chemical analyses showed that the crude protein and fat contents of the unprocessed (raw) seeds were 20.1% and 13.7%, respectively.

  9. Growth rates, biomass and distribution of selected woody plant roots in Burkea africana-Ochna pulchra savanna

    Microsoft Academic Search

    M. C. Rutherford

    1983-01-01

    Woody plants in an African Burkea africana-Ochna pulchra savanna on deep sandy soil were found to have characteristically bimorphic root systems. The shallow lateral root component was often well developed and roots extended up to seven times the extent of the plant canopy in several species. Exponential tapering of lateral roots was found in Terminalia sericea. The wide-ranging roots, together

  10. The habitat use and selection of African elephants (Loxodonta africana) in a land use landscape in Kenya, Africa

    E-print Network

    Hansen, Andrew J.

    The habitat use and selection of African elephants (Loxodonta africana) in a land use landscape) to examine how land use and cover types affect the distribution of African elephants in Kenya (2) to determine what land use cover types support larger densities of elephants as a very general indicate

  11. Environmental and maternal correlates of foetal sex ratios in the African buffalo (Syncerus caffer) and savanna elephant (Loxodonta africana)

    Microsoft Academic Search

    D. R. Visscher; R. J. van Aarde; I. Whyte

    2004-01-01

    Many species exhibit skewed sex ratios at birth. Here we investigate the relationships between environmental and maternal variables (as surrogates for maternal condition) and foetal sex in African buffalo Syncerus caffer and elephant Loxodonta africana of the Kruger National Park, South Africa. Using logistic regression no significant effect was found of year, maternal lactational status, maternal age, rainfall or density

  12. African elephants (Loxodonta africana) recognize visual attention from face and body orientation.

    PubMed

    Smet, Anna F; Byrne, Richard W

    2014-07-01

    How do animals determine when others are able and disposed to receive their communicative signals? In particular, it is futile to make a silent gesture when the intended audience cannot see it. Some non-human primates use the head and body orientation of their audience to infer visual attentiveness when signalling, but whether species relying less on visual information use such cues when producing visual signals is unknown. Here, we test whether African elephants (Loxodonta africana) are sensitive to the visual perspective of a human experimenter. We examined whether the frequency of gestures of head and trunk, produced to request food, was influenced by indications of an experimenter's visual attention. Elephants signalled significantly more towards the experimenter when her face was oriented towards them, except when her body faced away from them. These results suggest that elephants understand the importance of visual attention for effective communication. PMID:25013015

  13. Jared W. Young, CV. CURRICULUM VITAE

    E-print Network

    Squire, Larry R.

    , Department of Psychobiology EDUCATIONAL HISTORY 2001 ­ 2005: Ph.D. in Psychopharmacology, Department-clinical psychopharmacology: British Association for Psychopharmacology 2009: Travel Award: American College of Neuropsychopharmacology #12;Jared W. Young, CV. 2 2008: Outstanding poster presentation in preclinical psychopharmacology

  14. [Application of Tiantu (CV 22) in emergency].

    PubMed

    Zhu, Xian-Min; Huo, Shang-Fei; Lu, Lu; Wang, Xin-Zhi

    2013-06-01

    The operation and application of Tiantu (CV 22) in emergency are discussed in the paper. The stimulating methods at Tiantu (CV 22) are acupuncture and pressing technique. The correct insertion of needle and proper depth and direction of insertion are required during acupuncture. The pressing technique stress the pressing strength and pressing time. Acupuncture or pressing technique is suitable for the emergent measurement of asthma, asthmatic breathing, coma, blockage of phlegm, hiccup, sore throat, etc. It is indicated that Tiantu (CV 22) is the key point in the emergency and phlegm resolving. Based on the characteristics of the point as promoting qi circulation, reducing the reversed qi and resolving phlegm, in light of the proper points combination by different syndromes and in terms of the correct and safe stimulating methods, Tiantu (CV 22) can achieve the immediate therapeutic effects in the emergent situations. PMID:23967641

  15. SeeingWithOPenCV FindingFacesin lmages

    E-print Network

    Oh, Paul

    SeeingWithOPenCV FindingFacesin lmages what this mouthfulmeans.Figure1 showsan exampleof OPenCV severalscales. The classifierusesdata storedin an XMLfileto decidehow to classify each image location.The OPenCV andinstallingOpenCV,readingand writingimagefiles,capturingvideo, andworkingwiththelPllmagedata structufe. r

  16. Tutorials on Matlab, OpenCV, and Computer Science Department

    E-print Network

    Jiang, Hao

    Tutorials on Matlab, OpenCV, and SDL Computer Science Department Hao Jiang #12;Matlab · Matlab. · Rewrite bottleneck procedures using c. ­ System(`c_exe'); ­ Compile the function using mex. #12;OpenCV #12;OpenCV · OpenCV is a C library for image processing and computer vision. http

  17. Renal effects of Mammea africana Sabine (Guttiferae) stem bark methanol/methylene chloride extract on L-NAME hypertensive rats

    PubMed Central

    Nguelefack-Mbuyo, Elvine Pami; Dimo, Théophile; Nguelefack, Télesphore Benoit; Dongmo, Alain Bertrand; Kamtchouing, Pierre; Kamanyi, Albert

    2010-01-01

    Objective: The present study aims at evaluating the effects of methanol/methylene chloride extract of the stem bark of Mammea africana on the renal function of L-NAME treated rats. Material and Methods: Normotensive male Wistar rats were divided into five groups respectively treated with distilled water, L-NAME (40 mg/kg/day), L-NAME + L-arginine (100 mg/kg/day), L-NAME + captopril (20 mg/kg/day) or L-NAME + M. africana extract (200 mg/kg/day) for 30 days. Systolic blood pressure was measured before and at the end of treatment. Body weight was measured at the end of each week. Urine was collected 6 and 24 h after the first administration and further on day 15 and 30 of treatment for creatinine, sodium and potassium quantification, while plasma was collected at the end of treatment for the creatinine assay. ANOVA two way followed by Bonferonni or one way followed by Tukey were used for statistical analysis. Results: M. africana successfully prevented the rise in blood pressure and the acute natriuresis and diuresis induced by L-NAME. When given chronically, the extract produced a sustained antinatriuretic effect, a non-significant increase in urine excretion and reduced the glomerular hyperfiltration induced by L-NAME. Conclusions: The above results suggest that the methanol/methylene chloride extract of the stem bark of M. africana may protect kidney against renal dysfunction and further demonstrate that its antihypertensive effect does not depend on a diuretic or natriuretic activity. PMID:20927244

  18. Effect of alkaline treatment (‘akanwu’) and supplementary value of corn or crayfish on the protein quality of breadfruit (Treculia Africana)

    Microsoft Academic Search

    Ngozi Rose Nzomiwu

    1990-01-01

    The effect of treatment and supplementary value of corn (C) or crayfish (CR) on the protein quality of breadfruit (Treculia Africana) flours were studied in eighteen young rats. The 70?30 or 70?15?15 (Protein basis) combinations of breadfruit flours and corn or crayfish or both provided 1.6g N\\/100 g diet for the 35 day study. The addition of ‘akanwu’ to the

  19. Compositional, functional and storage properties of flours from raw and heat processed African breadfruit ( Treculia africana Decne) seeds

    Microsoft Academic Search

    Sunday Y. Giami; Matthew N. Adindu; Monday O. Akusu; Juliet N. T. Emelike

    2000-01-01

    African breadfruit (Treculia africana Decne) seeds were either boiled or roasted and then milled into flour. Chemical composition, functional properties and storage characteristics of raw and treated flours and the effect of partial proteolysis on selected functional properties of the raw flour were determined. Raw flour contained 20.1% crude protein, 2.5% total ash and 13.7% fat. Heat processing significantly (pp<0.05)

  20. Prunus africana (Hook.f.) Kalkman: the overexploitation of a medicinal plant species and its legal context.

    PubMed

    Bodeker, Gerard; van 't Klooster, Charlotte; Weisbord, Emma

    2014-11-01

    The linkage between herbal medicines and the sustainability of medical plants from which they are manufactured is increasingly being understood and receiving attention through international accords and trade labeling systems. However, little attention is paid to the fair trade aspects of this sector, including the issue of benefit-sharing agreements with traditional societies whose knowledge and resources are being exploited for commercial herbal medicine development and production. This article examines the case of Prunus africana (Hook.f.) Kalkman, from equatorial Africa. While the conservation and cultivation dimension of the trade in P. africana has been much discussed in literature, no research appears to have focused on the traditional resource rights and related ethical dimensions of this trade in traditional medicine of Africa. Serving as a cautionary tale for the unbridled exploitation of medicinal plants, the history of P. africana extraction is considered here in the context of relevant treaties and agreements existing today. These include the Nagoya Protocol, a supplementary agreement to the Convention on Biological Diversity, the Trade-Related Aspects of Intellectual Property Rights agreement from the World Trade Organization, and two African regional frameworks: the Swakopmund Protocol and the Organisation Africaine de la Propriété Intellectuelle Initiative. In the context of strengthening medicinal plant research in Africa, a novel international capacity-building project on traditional medicines for better public health in Africa will be discussed, illustrating how access and benefit sharing principles might be incorporated in future projects on traditional medicines. PMID:25225776

  1. Sharing fruit of Treculia africana among western gorillas in the Moukalaba-Doudou National Park, Gabon: preliminary report.

    PubMed

    Yamagiwa, Juichi; Tsubokawa, Keiko; Inoue, Eiji; Ando, Chieko

    2015-01-01

    We report the first 18 observed cases of fruit (Treculia africana) transfer among western lowland gorillas (Gorilla gorilla gorilla) in Moukalaba-Doudou National Park, Gabon. The fruit transfer occurred during our observations of a habituated group of gorillas in 2010 and 2013. Pieces of the fruits were transferred among adults and immatures, and three cases involved a silverback male. Once an individual picked up a fallen fruit of Treculia africana, other members of the group approached the possessor, who laid pieces of the fruits nearby and tolerated the others getting them. Agonistic interaction was rarely observed between the possessor and the non-possessor. Only the silverback male seemed to force another gorilla, a subadult male, to relinquish the fruit on the ground. He tolerated an adult female taking a piece of fruit on his leg and copulated with her on the following days. From these preliminary observations, most interactions over the fruit of Treculia africana among western gorillas in Moukalaba were not active transfer by the possessor but probably passive sharing. They were not only interpreted as a means of acquiring foraging skills by immatures (Nowell and Fletcher 2006) but also similar to behaviors observed in chimpanzees and bonobos in various social contexts. PMID:24962665

  2. Prunus africana (Hook.f.) Kalkman: The Overexploitation of a Medicinal Plant Species and Its Legal Context

    PubMed Central

    Bodeker, Gerard; Weisbord, Emma

    2014-01-01

    Abstract The linkage between herbal medicines and the sustainability of medical plants from which they are manufactured is increasingly being understood and receiving attention through international accords and trade labeling systems. However, little attention is paid to the fair trade aspects of this sector, including the issue of benefit-sharing agreements with traditional societies whose knowledge and resources are being exploited for commercial herbal medicine development and production. This article examines the case of Prunus africana (Hook.f.) Kalkman, from equatorial Africa. While the conservation and cultivation dimension of the trade in P. africana has been much discussed in literature, no research appears to have focused on the traditional resource rights and related ethical dimensions of this trade in traditional medicine of Africa. Serving as a cautionary tale for the unbridled exploitation of medicinal plants, the history of P. africana extraction is considered here in the context of relevant treaties and agreements existing today. These include the Nagoya Protocol, a supplementary agreement to the Convention on Biological Diversity, the Trade-Related Aspects of Intellectual Property Rights agreement from the World Trade Organization, and two African regional frameworks: the Swakopmund Protocol and the Organisation Africaine de la Propriété Intellectuelle Initiative. In the context of strengthening medicinal plant research in Africa, a novel international capacity-building project on traditional medicines for better public health in Africa will be discussed, illustrating how access and benefit sharing principles might be incorporated in future projects on traditional medicines. PMID:25225776

  3. SegCV : traitement efficace de CV avec analyse et correction d'erreurs

    E-print Network

    sur une grande collection de CV en français avec correction du bruit montrent de bons résultats en, Ressources humaines, traitement de CV, Modèle à base de règles. KEYWORDS: Information Retrieval, Human

  4. Fine-scale spatial genetic dynamics over the life cycle of the tropical tree Prunus africana.

    PubMed

    Berens, D G; Braun, C; González-Martínez, S C; Griebeler, E M; Nathan, R; Böhning-Gaese, K

    2014-11-01

    Studying fine-scale spatial genetic patterns across life stages is a powerful approach to identify ecological processes acting within tree populations. We investigated spatial genetic dynamics across five life stages in the insect-pollinated and vertebrate-dispersed tropical tree Prunus africana in Kakamega Forest, Kenya. Using six highly polymorphic microsatellite loci, we assessed genetic diversity and spatial genetic structure (SGS) from seed rain and seedlings, and different sapling stages to adult trees. We found significant SGS in all stages, potentially caused by limited seed dispersal and high recruitment rates in areas with high light availability. SGS decreased from seed and early seedling stages to older juvenile stages. Interestingly, SGS was stronger in adults than in late juveniles. The initial decrease in SGS was probably driven by both random and non-random thinning of offspring clusters during recruitment. Intergenerational variation in SGS could have been driven by variation in gene flow processes, overlapping generations in the adult stage or local selection. Our study shows that complex sequential processes during recruitment contribute to SGS of tree populations. PMID:24849171

  5. Microorganisms associated with natural fermentation of Prosopis africana seeds for the production of okpiye.

    PubMed

    Achi, O K

    1992-10-01

    Okpiye is a food condiment prepared by the fermentation of Prosopis africana seeds. The traditional process for the production and microbiological characteristics of the condiment were investigated. During laboratory fermentation that lasted 96 h, the mesquite seeds underwent a natural fermentation that was characterised by the growth of microorganisms to 10(6)-10(8) cfu/g. Several species of bacteria especially B. subtilis, B. licheniformis, B. megaterium, Staphylococcus epidermidis and Micrococcus spp were found to be the most actively involved organisms. However, significant contributions to the microbial ecology were made by Enterobacter cloacae and Klebsiella pneumoniae. Lactobacillus spp were present in low numbers towards the end of the fermentation. The presence of Proteus and Pseudomonas spp in traditional fermented samples demonstrate the variability which may exist in the microflora of individual fermented samples. Variations in the important microbial groups show that Bacillus spp were the most prevalent species and occurred until the end of fermentation. Temperature, pH and titratable acidity varied with time and were influenced by the metabolic activities of the microorganisms. PMID:1438073

  6. Wild female African elephants (Loxodonta africana) exhibit personality traits of leadership and social integration.

    PubMed

    Lee, Phyllis C; Moss, Cynthia J

    2012-08-01

    Animal personalities have been demonstrated for almost 200 species, with stable dimensions of responses (aggressive to fearful; shy to bold) across contexts and with a heritable basis to these traits. As a long-lived and highly social species, elephants (Loxodonta africana) were expected to demonstrate complex dimensions to individual characteristics or personalities, which would be obvious to human observers and validated by behavioral observations. We used principal-components analysis of ratings on 26 behavioral adjectives applied to one social unit, coded as the EB family, which has been observed for 38 years. Eleven adult females were rated by four observers and found to have individually variable traits on four dimensions described by principal-components analysis. The first component was associated with effective and confident family leadership. Component 2 was age-related, and defined by playfulness, exploration and high levels of activity, suggesting both an experience and an age-related element to its structure. Component 3 represented gentleness and at its other extreme, aggression, and Component 4 was related to constancy (predictability and popularity), with both of these latter components reflecting social integration. Leadership among elephant females represents the successful negotiation among individual interests, and our components were related to a capacity to affect the behavior of others in the absence of aggressive dominance. The family matriarch, Echo, was high on elements associated with leadership. The importance of the matriarch in this family's success suggests that elements of personality may underlie interfamilial variation in long-term survival and reproduction. PMID:22905995

  7. Assessment of ovarian cycles in the African elephant (Loxodonta africana) by measurement of salivary progesterone metabolites.

    PubMed

    Illera, Juan-Carlos; Silván, Gema; Cáceres, Sara; Carbonell, Maria-Dolores; Gerique, Cati; Martínez-Fernández, Leticia; Munro, Coralie; Casares, Miguel

    2014-01-01

    Monitoring ovarian cycles through hormonal analysis is important in order to improve breeding management of captive elephants, and non-invasive collection techniques are particularly interesting for this purpose. However, there are some practical difficulties in collecting proper samples, and easier and more practical methods may be an advantage for some institutions and/or some animals. This study describes the development and validation of an enzymeimmunoassay (EIA) for progestins in salivary samples of African elephants, Loxodonta africana. Weekly urinary and salivary samples from five non-pregnant elephant cows aged 7-12 years were obtained for 28 weeks and analyzed using EIA. Both techniques correlated positively (r = 0.799; P < 0.001), and the cycle characteristics obtained were identical. The results clearly show that ovarian cycles can be monitored by measuring progestins from salivary samples in the African elephant. This is a simple and non-invasive method that may be a practical alternative to other sampling methods used in the species. PMID:24610639

  8. Hynek CV, Page 1 CURRICULUM VITAE

    E-print Network

    Mojzsis, Stephen J.

    has focused on the geologic, geochemical, hydrologic, and climatic evolutions of the planet Mars. He properties of Mars, impact craters, and the physics of volcanic eruptions on Earth and other planets. Brian time on Mars and determine the history of water and volcanism on the Red Planet. #12;Hynek CV, Page 2

  9. CV De Juan Vigaray CURRICULUM VITAE

    E-print Network

    Escolano, Francisco

    by the Market Team, Barcelona (Spain): "Retail Sales Promotion & Merchandising". Fall 1994 Teaching AssistantCV De Juan Vigaray 1 CURRICULUM VITAE María D. De Juan Vigaray, PhD RESEARCH INTERESTS TEACHING Spain Phone: + 00 34 9 6 590 34 00 (ext. 3167) Fax: + 00 34 (9) 6 512 61 20 E-mail: mayo

  10. CV (Mercado). February 2005 NORMAN MERCADO SILVA

    E-print Network

    Mercado-Silva, Norman

    CV (Mercado). February 2005 NORMAN MERCADO SILVA Center for Limnology University of Wisconsin - Madison; 2001. PhD: Zoology and Limnology, Department of Zoology, University of Wisconsin ­ Madison, 2005 - Postdoctoral Associate. Center for Limnology (University of Wisconsin - Madison) 2004 - 05 Research Assistant

  11. The Limbic-Prefrontal Network Modulated by Electroacupuncture at CV4 and CV12

    PubMed Central

    Fang, Jiliang; Wang, Xiaoling; Liu, Hesheng; Wang, Yin; Zhou, Kehua; Hong, Yang; Liu, Jun; Wang, Lei; Xue, Chao; Song, Ming; Liu, Baoyan; Zhu, Bing

    2012-01-01

    fMRI studies showed that acupuncture could induce hemodynamic changes in brain networks. Many of these studies focused on whether specific acupoints could activate specific brain regions and were often limited to manual acupuncture at acupoints on the limbs. In this fMRI study, we investigated acupuncture's modulation effects on brain functional networks by electroacupuncture (EA) at acupoints on the midline of abdomen. Acupoints Guanyuan (CV4) and Zhongwan (CV12) were stimulated in 21 healthy volunteers. The needling sensations, brain activation, and functional connectivity were studied. We found that the limbic-prefrontal functional network was deactivated by EA at CV4 and CV12. More importantly, the local functional connectivity was significantly changed during EA stimulation, and the change persisted during the period after the stimulation. Although minor differences existed, both acupoints similarly modulated the limbic-prefrontal functional network, which is overlapped with the functional circuits associated with emotional and cognitive regulation. PMID:22291848

  12. Effect of pre-dehulling treatments on the composition of seeds of the legume Afzelia africana and its potential use in pastries.

    PubMed

    Onweluzo, J C; Morakinyo, A O

    1997-01-01

    Flour was prepared from seeds of Afzelia africana dehulled by different treatments and used to replace 10, 20, 30, 40 and 50% wheat flour in biscuits and doughnuts. The composition and water and oil absorption properties of the flour blends were evaluated. The biscuits and doughnuts made from each flour blend were evaluated organoleptically. The composite flour containing the highest proportion (50%) of A. africana seed flour contained the highest levels of protein and fat, exhibited the highest water absorption property but the lowest oil absorption capacity. Sensory scores showed high overall acceptability for products with a 10-30% level of substitution. PMID:9373871

  13. A simple method of providing intermittent positive-pressure ventilation to etorphine-immobilized elephants (Loxodonta africana) in the field.

    PubMed

    Horne, W A; Tchamba, M N; Loomis, M R

    2001-12-01

    Five African elephants (Loxodonta africana) were immobilized with etorphine in Waza National Park, Cameroon, for the purpose of deploying radio/satellite tracking collars. A portable ventilator constructed from two high-flow demand valves and the Y-piece of a large animal anesthesia circuit was used to provide intermittent positive-pressure ventilation with 100% oxygen. Oxygenation status improved dramatically in all five elephants. In one hypoxemic elephant, arterial PaO2 increased from 40 to 366 mm Hg. The results of this study demonstrate that both oxygenation and ventilation can be readily controlled in etorphine-immobilized elephants even under remote field conditions. PMID:12785710

  14. SeeingWithOpenCV FollowThatFace!

    E-print Network

    Oh, Paul

    SeeingWithOpenCV FollowThatFace! describedin lastmonth'sarticle.To do that, however,you'd needto'dnepdto handlethatJ v t v u situation,aswell. Fortunately,OpenCV includes specializedcode for trackinga faceCVtotrackataceonce you'vedetectedit. FaceTrackingin OpenCV Trackinga face is more drfficult thantrackinga strongly

  15. The OpenCV Library: Computing Optical Flow

    E-print Network

    Thrun, Sebastian

    1 The OpenCV Library: Computing Optical Flow David Stavens Stanford Artificial Intelligence Lab I optical flow algorithm! Plan OpenCV Basics What is it? How do we get started? Feature Finding and Optical Flow A brief mathematical discussion. OpenCV Implementation of Optical Flow Step by step. #12;3 What

  16. Lack of spatial and behavioral responses to immunocontraception application in African elephants (Loxodonta africana).

    PubMed

    Delsink, Audrey K; Kirkpatrick, Jay; van Altena, J J; Bertschinger, Henk J; Ferreira, Sam M; Slotow, Robert

    2013-12-01

    Opinions are divided as to whether human intervention to control elephant (Loxodonta africana) population growth is desirable, partly because of elephant welfare concerns. Female contraception through immunization with porcine zona pellucida (PZP) proteins is viable. The effects of sustained use and application of the PZP vaccine on elephant behavioral and spatial responses were examined by evaluating herd ranging, fission-fusion dynamics, association patterns, and reproductive and sexual behaviors. Minimal change was anticipated as a result of long calf dependence on and association with cows, a reduced but not indefinite 0% growth rate and the known mechanism of action of PZP vaccines, and minimal expected change in resource requirements necessitating behavioral or spatial use adaptations. Although behavioral effects identified in previous hormonal contraceptive trials were evident, it was demonstrated that immunocontraception caused no prolonged behavioral, social, or spatial changes over the 11-yr study period. Individually identified elephants were monitored from 1999 to 2011. Minimal, short-term social disruption, with temporary changes to the herds' core ranges, was observed during the annual treatment events, particularly in the first three treatment years, when vaccinations were conducted exclusively from the ground. Thereafter, when vaccinations were conducted aerially, minor disruptions were confined to the morning of administration only. Despite sustained treatments resulting in demographic changes of fewer calves being born, treatments did not alter spatial range use, and no adverse interherd-intraherd relations were observed. Similarly, resource requirements did not change as calving still occurred, although in fewer numbers. It was concluded that PZP immunocontraception has no detectable behavioral or social consequences in elephants over the course of 11 yr, providing a convincing argument for the use of sustained immunocontraception in the medium to long term as an important tool for elephant management. Behavioral consequences of alternative management approaches should all receive similar scrutiny to enable managers to make informed decisions when weighing management interventions. PMID:24437086

  17. Molecular Characterization of Adipose Tissue in the African Elephant (Loxodonta africana)

    PubMed Central

    Choong, Siew S.; Giles, Thomas C.; Sells, James; May, Sean; Stansfield, Fiona J.; Allen, William R.; Emes, Richard D.; Mostyn, Alison; Mongan, Nigel P.; Yon, Lisa

    2014-01-01

    Adipose tissue (AT) is a dynamic and flexible organ with regulatory roles in physiological functions including metabolism, reproduction and inflammation; secreted adipokines, including leptin, and fatty acids facilitate many of these roles. The African elephant (Loxodonta africana) is experiencing serious challenges to optimal reproduction in captivity. The physiological and molecular basis of this impaired fertility remains unknown. AT production of leptin is a crucial molecular link between nutritional status, adiposity and fertility in many species. We propose that leptin has a similar function in the African elephant. African elephant visceral and subcutaneous adipose tissue (AT) was obtained from both sexes and a range of ages including females with known pregnancy status. RNA was extracted and histological sections created and analyzed by microarray, PCR and immunohistochemistry respectively. Gas-chromatography was used to determine the fatty acid composition of AT. Microarray expression profiling was used to compare gene expression profiles of AT from pre-pubertal versus reproductively competent adult African elephants. This study demonstrates, for the first time, leptin mRNA and protein expression in African elephant AT. The derived protein sequence of the elephant leptin protein was exploited to determine its relationship within the class I helical cytokine superfamily, which indicates that elephant leptin is most closely related to the leptin orthologs of Oryctolagus cuniculus (European rabbit), Lepus oiostolus (woolly hare), and members of the Ochotonidae (Pika). Immunohistological analysis identified considerable leptin staining within the cytoplasm of adipocytes. Significant differences in fatty acid profiles between pregnant and non-pregnant animals were revealed, most notably a reduction in both linoleic and ? linoleic acid in pregnant animals. This report forms the basis for future studies to address the effect of nutrient composition and body condition on reproduction in captive and wild elephants. PMID:24633017

  18. Eradication of elephant ear mites (Loxoanoetus bassoni) in two African elephants (Loxodonta africana).

    PubMed

    Wyatt, Jeff; DiVincenti, Louis

    2012-03-01

    Elephant ear mites, not previously described in North America, were eradicated in two African elephants (Loxodonta africana) after six otic instillations of ivermectin at 2-wk intervals. The microscopic examination of a clear, mucoid discharge collected from the external ear canals of two wild-born African elephants housed in a New York State zoo for 25 yr revealed live mites (Loxoaneotus bassoni). The cytologic examination demonstrated no evidence of inflammation or infection. Both elephants were asymptomatic with normal hemograms and serum chemistry panels. A diagnosis of otoacariasis was made. Each elephant was treated six times with 5 ml of 1% ivermectin syrup instilled in each ear canal once every 2 wk. Microscopic examinations of clear mucus collected from each elephant's ear canals 9 days after the first instillation of ivermectin were negative for any life stages of ear mites. Microscopic examinations of mucus collected from both elephants' ear canals at 6, 11, and 16 wk, as well as annually post-treatment for 7 yr, confirmed eradication of the ear mites. The L. bassoni ear mite was first identified in the external ear canals of wild, asymptomatic, lesion-free, African elephants culled in Kruger National Park in South Africa. However, a new species in the same genus of mites (Loxoanoetus lenae) was identified at the necropsy of an 86-yr-old Asian elephant (Elephas maximus) living in a circus in Australia. The autopsy revealed a marked, ballooning distension of bone around the left external acoustic meatus, suggestive of mite-induced otitis externa, as seen in cattle infested with ear mites (Raillieta auris). Elephant health care providers should identify the prevalence of, and consider treatment of, elephants in their care infested with ear mites, given the possible risk for adverse health effects. PMID:22448521

  19. Video Encryption Based on OpenCV

    Microsoft Academic Search

    Cunyan Meng; Xinghui Zhang

    2010-01-01

    This paper uses the open source computer vision library (OpenCV for short) as basic library and calls library functions to achieve real-time video capture and output. We narrate the process of making the matrix interpretable as an image through analyzing the data structure of IplImage and meaning of its member variable. The library functions to read video take the video

  20. EARTH, MOON, SUN, AND CV ACCRETION DISKS

    SciTech Connect

    Montgomery, M. M. [Department of Physics, University of Central Florida, Orlando, FL 32816 (United States)

    2009-11-01

    Net tidal torque by the secondary on a misaligned accretion disk, like the net tidal torque by the Moon and the Sun on the equatorial bulge of the spinning and tilted Earth, is suggested by others to be a source to retrograde precession in non-magnetic, accreting cataclysmic variable (CV) dwarf novae (DN) systems that show negative superhumps in their light curves. We investigate this idea in this work. We generate a generic theoretical expression for retrograde precession in spinning disks that are misaligned with the orbital plane. Our generic theoretical expression matches that which describes the retrograde precession of Earths' equinoxes. By making appropriate assumptions, we reduce our generic theoretical expression to those generated by others, or to those used by others, to describe retrograde precession in protostellar, protoplanetary, X-ray binary, non-magnetic CV DN, quasar, and black hole systems. We find that spinning, tilted CV DN systems cannot be described by a precessing ring or by a precessing rigid disk. We find that differential rotation and effects on the disk by the accretion stream must be addressed. Our analysis indicates that the best description of a retrogradely precessing spinning, tilted, CV DN accretion disk is a differentially rotating, tilted disk with an attached rotating, tilted ring located near the innermost disk annuli. In agreement with the observations and numerical simulations by others, we find that our numerically simulated CV DN accretion disks retrogradely precess as a unit. Our final, reduced expression for retrograde precession agrees well with our numerical simulation results and with selective observational systems that seem to have main-sequence secondaries. Our results suggest that a major source to retrograde precession is tidal torques like that by the Moon and the Sun on the Earth. In addition, these tidal torques should be common to a variety of systems where one member is spinning and tilted, regardless if accretion disks are present or not. Our results suggest that the accretion disk's geometric shape directly affects the disk's precession rate.

  1. Investigation of minor species Candida africana, Candida stellatoidea and Candida dubliniensis in the Candida albicans complex among Yaoundé (Cameroon) HIV-infected patients.

    PubMed

    Ngouana, Thierry K; Krasteva, Donika; Drakulovski, Pascal; Toghueo, Rufin K; Kouanfack, Charles; Ambe, Akaba; Reynes, Jacques; Delaporte, Eric; Boyom, Fabrice F; Mallié, Michèle; Bertout, Sébastien

    2015-01-01

    Minor species of the Candida albicans complex may cause overestimation of the epidemiology of C. albicans, and misidentifications could mask their implication in human pathology. Authors determined the occurrence of minor species of the C. albicans complex (C. africana, C. dubliniensis and C. stellatoidea) among Yaoundé HIV-infected patients, Cameroon. Stool, vaginal discharge, urine and oropharyngeal samples were analysed by mycological diagnosis. Isolates were identified by conventional methods and mass spectrometry (MS; carried out by the matrix-assisted laser desorption-ionisation time-of-flight MS protocol). Candida albicans isolates were thereafter submitted to the PCR amplification of the Hwp1 gene. The susceptibility of isolates to antifungal drugs was tested using the Clinical and Laboratory Standards Institute M27-A3 protocol. From 115 C. albicans obtained isolates, neither C. dubliniensis nor C. stellatoidea was observed; two strains of C. africana (422PV and 448PV) were identified by PCR electrophoretic profiles at 700 bp. These two C. africana strains were vaginal isolates. The isolate 448PV was resistant to ketoconazole at the minimal inhibitory concentration of 2 ?g ml(-1), and showed reduced susceptibility to amphotericin B at 1 ?g ml(-1). This first report on C. africana occurrence in Cameroon brings clues for the understanding of the global epidemiology of this yeast as well as that of minor species of the C. albicans complex. PMID:25289589

  2. Microbial Studies and Biochemical Characteristics of Controlled Fermented Afiyo- a Nigerian Fermented Food Condiment from Prosopis africana (Guill and Perr.) Taub

    Microsoft Academic Search

    Adenike A. O. Ogunshe; Mopelola O. Omotosho; A. D. V. Ayansina

    2007-01-01

    3 Abstract: One hundred and fifteen bacterial strains isolated from fermenting Prosopis africana during a controlled production of okpehe, a Nigerian food-seasoning condiment most popular among the middle belt states of Nigeria were characterized as Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, Bacillus megaterium, non-sporing Staphylococcus species and Escherichia coli according to their differences in morphological, microscopic and biochemical characteristics using

  3. ANALYSIS OF CLAVICEPS AFRICANA AND C. SORGHI FROM INDIA USING AFLPS, EF-1 ALPHA GENE INTRON 4, AND BETA-TUBULIN GENE INTRON 3

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sorghum ergot is a serious constraint in the production of F1hybrid seeds, an essential input in productive sorghum cultivation systems. Until 1991, Claviceps sorghi was considered to be the only perfect stage of Sphacelia sorghi McRae found to occur in India. In 1990, Claviceps africana, another p...

  4. In vitro studies to assess the antioxidative, radical scavenging and arginase inhibitory potentials of extracts from Artocarpus altilis, Ficus exasperate and Kigelia africana

    PubMed Central

    Akanni, Olubukola Oyebimpe; Owumi, Solomon Eduviere; Adaramoye, Oluwatosin Adekunle

    2014-01-01

    Objective To justify the use of Artocarpus altilis (A. altilis), Ficus exasperata (F. exasperata) and Kigelia africana (K. africana) in ethnomedicine for the treatment of several ailments and to evaluate the in vitro antioxidant, radical scavenging and arginase inhibitory potentials of these herbs and compared with catechin (Standard). Methods Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide (H2O2) and hydroxyl (OH) radicals scavenging methods. The flavonoids and phenolics content, inhibition of arginase activity, Fe2+/ascorbate-induced lipid peroxidation (LPO) and reducing power were also determined. Results The A. altilis, F. exasperata and K. africana showed dose-dependent and significant scavenging of DPPH, H2O2 and OH radicals in vitro relative to catechin. The A. altilis and F. exasperata effectively scavenged DPPH radical with IC50 of 593 and 635 µg/mL and, OH radical with IC50 of 487 and 514 µg/mL, respectively. The DPPH and OH radicals scavenging activities followed the order A. altilis>F. exasperata>K. africana. In addition, A. altilis and F. exasperata significantly (P<0.05) inhibited LPO in a dose-dependent manner. The A. altilis extract had the most potent inhibitory activity against LPO with 79% relative to catechin (28%) at 750 µg/mL. The reducing power followed the order: A. altilis>Catechin>F. exasperata>K. africana at 1?000 µg/mL. The A. altilis at 500 and 750 µg/mL significantly (P<0.05) inhibited arginase activity by 63% and 67%, respectively. The flavonoids contents were found to be highest in A. altilis. Conclusions Extracts of A. altilis and F. exasperata are potent antioxidative agents with strong radical scavenging activity and inhibition of lipid peroxidation. PMID:25183137

  5. Fusarium inhibition by wild populations of the medicinal plant Salvia africana-lutea L. linked to metabolomic profiling

    PubMed Central

    2014-01-01

    Background Salvia africana-lutea L., an important medicinal sage used in the Western Cape (South Africa), can be termed a ‘broad-spectrum remedy’ suggesting the presence of a multiplicity of bioactive metabolites. This study aimed at assessing wild S. africana-lutea populations for chemotypic variation and anti-Fusarium properties. Methods Samples were collected from four wild growing population sites (Yzerfontein, Silwerstroomstrand, Koeberg and Brackenfell) and one garden growing location in Stellenbosch. Their antifungal activities against Fusarium verticillioides (strains: MRC 826 and MRC 8267) and F. proliferatum (strains: MRC 6908 and MRC 7140) that are aggressive mycotoxigenic phytopathogens were compared using an in vitro microdilution assay. To correlate antifungal activity to chemical profiles, three techniques viz. Gas chromatography-mass spectrometry (GC-MS); Liquid chromatography-mass spectrometry (LC-MS) and 1H Nuclear Magnetic Resonance (NMR) were employed. Principal Component Analysis (PCA) was applied to the NMR data. The partial least squares-discriminant analysis (PLS-DA) was used to integrate LC-MS and NMR data sets. All statistics were performed with the SIMCA-P?+?12.0 software. Results The dichloromethane:methanol (1:1; v/v) extracts of the plant species collected from Stellenbosch demonstrated the strongest inhibition of F. verticillioides and F. proliferatum with minimum inhibitory concentration (MIC) values of 0.031 mg ml-1 and 0.063 mg ml-1 respectively. GC-MS showed four compounds which were unique to the Stellenbosch extracts. By integrating LC-MS and 1H NMR analyses, large chemotype differences leading to samples grouping by site when a multivariate analysis was performed, suggested strong plant-environment interactions as factors influencing metabolite composition. Signals distinguishing the Stellenbosch profile were in the aromatic part of the 1H NMR spectra. Conclusions This study shows the potential of chemotypes of Salvia africana-lutea in controlling fungal growth and consequently mycotoxin production. Products for use in the agricultural sector may be developed from such chemotypes. PMID:24621275

  6. Attenuation of oxidative stress in U937 cells by polyphenolic-rich bark fractions of Burkea africana and Syzygium cordatum

    PubMed Central

    2013-01-01

    Background Oxidative stress has been implicated in the progression of various diseases, which may result in the depletion of endogenous antioxidants. Exogenous supplementation with antioxidants could result in increased protection against oxidative stress. As concerns have been raised regarding synthetic antioxidant usage, the identification of alternative treatments is justified. The aim of the present study was to determine the antioxidant efficacy of Burkea africana and Syzygium cordatum bark extracts in an in vitro oxidative stress model. Methods Cytotoxicity of crude aqueous and methanolic extracts, as well as polyphenolic-rich fractions, was determined in C2C12 myoblasts, 3T3-L1 pre-adipocytes, normal human dermal fibroblasts and U937 macrophage-like cells using the neutral red uptake assay. Polyphenolic content was determined using the Folin-Ciocalteau and aluminium trichloride assays, and antioxidant activity using the Trolox Equivalence Antioxidant Capacity and DPPH assays. The extracts efficacy against oxidative stress in AAPH-exposed U937 cells was assessed with regards to reactive oxygen species generation, cytotoxicity, apoptosis, lipid peroxidation and reduced glutathione depletion. Results B. africana and S. cordatum showed enrichment of polyphenols from the aqueous extract, to methanolic extract, to polyphenolic-rich fractions. Antioxidant activity followed the same trend, which correlated well with the increased concentration of polyphenols, and was between two- to three-fold stronger than the Trolox antioxidant control. Both plants had superior activity compared to ascorbic acid in the DPPH assay. Polyphenolic-rich fractions were most toxic to the 3T3-L1 (IC50’s between 13 and 21 ?g/ml) and C2C12 (IC50’s approximately 25 ?g/ml) cell lines, but were not cytotoxic in the U937 and normal human dermal fibroblasts cultures. Free radical-induced generation of reactive oxygen species (up to 80%), cytotoxicity (up to 20%), lipid peroxidation (up to 200%) and apoptosis (up to 60%) was successfully reduced by crude extracts of B. africana and the polyphenolic-rich fractions of both plants. The crude extracts of S. cordatum were not as effective in reducing cytotoxic parameters. Conclusion Although oxidative stress was attenuated in U937 cells, cytotoxicity was observed in the 3T3-L1 and C2C12 cell lines. Further isolation and purification of polyphenolic-fractions could increase the potential use of these extracts as supplements by decreasing cytotoxicity and maintaining antioxidant quality. PMID:23714009

  7. Ch OpenCV for interactive open architecture computer vision

    Microsoft Academic Search

    Qingcang Yu; Harry H. Cheng; Wayne W. Cheng; Xiaodong Zhou

    2004-01-01

    In this paper, design and implementation of an interactive open architecture computer vision software package called Ch OpenCV is presented. Benefiting from both Ch and OpenCV, Ch OpenCV has many salient features. It is interactive, capable of interface with binary static or dynamical C\\/C++ libraries, integrated with advanced numerical features and embeddable. It is especially suitable for rapid prototyping, web-based

  8. MIZEX, 1984, NASA CV-990 flight report

    NASA Technical Reports Server (NTRS)

    1985-01-01

    During June/July 1984, the NASA CV-990 Airborne Laboratory was utilized in a mission to overly the Fram Strait/East Greenland Sea marginal ice zone (MIZ) during the main summer marginal ice zone experiment (MIZEX '84). The eight data flights were coordinated where possible with overpasses of the Nimbus-7 satellite, and with measurement of sea ice, open ocean, and atmospheric properties at the surface. The surface research teams were based on seven research vessels, some with helicopters: (1) M/V Kvitbjorn, (2) M/V Polarqueen; (3) M/S Haakon Mosby; (4) a M/S H.U. Sverdrup, all from Norway; (5) F/S Polarstern from the Federal Republic of Germany; and (6) the USNS Lynch from the USA. There were also coordinated flights with the NRL P3, NOAA P3, Canadian CV580, and the French B-17 during the overlap portions of their respective missions. Analysis of the real-time data acquired during the mission and uncalibrated data stored on tape has served to indicate the mission was over 90% successful.

  9. K-Band Observations of Subgap CV's

    NASA Astrophysics Data System (ADS)

    Hamilton, Ryan T.; Harrison, T. E.; Tappert, C.; Howell, S. B.

    2010-01-01

    We present the continuing results of VLT ISAAC K-band spectroscopy of short period cataclysmic variable (CV) systems below the "period gap” between 2 to 3 hours. It is extremely difficult to see the secondary stars in short period systems, since the low luminosity secondaries are swamped by the accretion disks in these objects. We show the infrared spectra for 9 systems below the gap: V2051 Oph, V436 Cen, EX Hya, VW Hyi, Z Cha, WX Hyi, V893 Sco, RZ Leo, and TY PsA. We are able to clearly detect the secondary stars in all but WX Hyi, V893 Sco, and TY PsA. We present the first direct detections of the secondary stars of V2051 Oph and V436 Cen, and present new detections for EX Hya, VW Hyi, Z Cha, and RZ Leo. Previous infrared spectroscopic surveys of CVs above the period gap reveal that these secondaries suffer a universal deficit of C12, enhanced levels of C13, and unusual abundance patterns for other species (e.g., Mg,Si, Al, Ca). Infrared spectroscopy of short period magnetic CVs (polars) have shown that their secondary stars appear completely normal, and the secondaries in a sample of "pre-CVs" have normal carbon abundances. Implications for CV evolution and formation scenarios will be discussed.

  10. Divergent pattern of nuclear genetic diversity across the range of the Afromontane Prunus africana mirrors variable climate of African highlands

    PubMed Central

    Kadu, Caroline A. C.; Konrad, Heino; Schueler, Silvio; Muluvi, Geoffrey M.; Eyog-Matig, Oscar; Muchugi, Alice; Williams, Vivienne L.; Ramamonjisoa, Lolona; Kapinga, Consolatha; Foahom, Bernard; Katsvanga, Cuthbert; Hafashimana, David; Obama, Crisantos; Geburek, Thomas

    2013-01-01

    Background and Aims Afromontane forest ecosystems share a high similarity of plant and animal biodiversity, although they occur mainly on isolated mountain massifs throughout the continent. This resemblance has long provoked questions on former wider distribution of Afromontane forests. In this study Prunus africana (one of the character trees of Afromontane forests) is used as a model for understanding the biogeography of this vegetation zone. Methods Thirty natural populations from nine African countries covering a large part of Afromontane regions were analysed using six nuclear microsatellites. Standard population genetic analysis as well as Bayesian and maximum likelihood models were used to infer genetic diversity, population differentiation, barriers to gene flow, and recent and all migration among populations. Key Results Prunus africana exhibits strong divergence among five main Afromontane regions: West Africa, East Africa west of the Eastern Rift Valley (ERV), East Africa east of the ERV, southern Africa and Madagascar. The strongest divergence was evident between Madagascar and continental Africa. Populations from West Africa showed high similarity with East African populations west of the ERV, whereas populations east of the ERV are closely related to populations of southern Africa, respectively. Conclusions The observed patterns indicate divergent population history across the continent most likely associated to Pleistocene changes in climatic conditions. The high genetic similarity between populations of West Africa with population of East Africa west of the ERV is in agreement with faunistic and floristic patterns and provides further evidence for a historical migration route. Contrasting estimates of recent and historical gene flow indicate a shift of the main barrier to gene flow from the Lake Victoria basin to the ERV, highlighting the dynamic environmental and evolutionary history of the region. PMID:23250908

  11. Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692T) from the alkaline Lake Magadi in the East African Rift

    SciTech Connect

    Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Abt, Birte [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Scheuner, Carmen [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Teshima, Hazuki [Los Alamos National Laboratory (LANL); Held, Brittany [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Tindall, Brian [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute

    2013-01-01

    Spirochaeta africana Zhilina et al. 1996 is an anaerobic, aerotolerant, spiral-shaped bacte- rium that is motile via periplasmic flagella. The type strain of the species, Z-7692T, was iso- lated in 1993 or earlier from a bacterial bloom in the brine under the trona layer in a shallow lagoon of the alkaline equatorial Lake Magadi in Kenya. Here we describe the features of this organism, together with the complete genome sequence, and annotation. Considering the pending reclassification of S. caldaria to the genus Treponema, S. africana is only the second 'true' member of the genus Spirochaeta with a genome-sequenced type strain to be pub- lished. The 3,285,855 bp long genome of strain Z-7692T with its 2,817 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  12. Joint effect of halides and ethanol extract of Lasianthera africana on inhibition of corrosion of mild steel in H 2 SO 4

    Microsoft Academic Search

    N. O. Eddy; S. A. Odoemelam; A. O. Odiongenyi

    2009-01-01

    Inhibitive and adsorption properties of ethanol extract of Lasianthera africana for inhibition of corrosion of mild steel in H2SO4 were studied using gravimetric, thermometric, gasometric, and infrared (IR) methods. The extract was found to be a good inhibitor\\u000a of corrosion of mild steel in H2SO4. Inhibitive properties of the extract were attributed to enhancement in adsorption of the inhibitor on

  13. Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on streptozotocin-induced diabetic Wistar rats

    PubMed Central

    Oyedemi, SO; Adewusi, EA; Aiyegoro, OA; Akinpelu, DA

    2011-01-01

    Objective To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana (A. africana) and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats. Methods A total of 30 rats including 24 diabetic and 6 normal rats were used for this study. Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin. After being confirmed diabetic, animals were orally treated with distilled water or extracts at 100 or 200 mg/kg body weight daily for 10 days. The haematological parameters including red blood and white blood cells and their functional indices were evaluated in diabetic treated groups compared with the controls. Results The extract significantly reduced the blood glucose levels while the best result was obtained at 200 mg/kg body weight. The feed and water intake in diabetic rats were significantly reduced while weight loss was minimized at both dosages. Similarly, the levels of red blood, white blood cells and their functional indices were significantly improved after extract administration at both doses. Conclusions It can be concluded that the aqueous extract of bark of A. africana possesses antihyperglycemic properties. In addition, the extract can prevent various complications of diabetes and improve some haematological parameters. Further experimental investigation is needed to exploit its relevant therapeutic effect to substantiate its ethnomedicinal usage. PMID:23569792

  14. Cactodera eremica n. sp., Afenestrata africana (Luc et al., 1973) n. gen., n. comb., and an Emended Diagnosis of Sarisodera Wouts and Sher, 1971 (Heteroderidae)

    PubMed Central

    Baldwin, J. G.; Bell, A. H.

    1985-01-01

    Systematic contributions to Heteroderidae include description of Cactodera eremica n. sp., an emended diagnosis of Sarisodera Wouts and Sher, 1971, and proposal of a new genus and new combination, Afenestrata africana (synonym Sarisodera africana Luc et al., 1973). Cactodera eremica, from the roots of shadscale in Utah, most closely resembles Cactodera thornei (Golden and Raski, 1977) but differs by the presence of a finely striated cuticle, a fine surface pattern on eggs, a shorter female stylet, distance of the DGO from the stylet, vulval slit, and smaller diameter of circumfenestra, as well as a shorter tail in second-stage juveniles. The response of the host to C. eremica is similar to other Heterodera sensu lato including a large syncytium with wall ingrowths. The diagnosis of Sarisodera is emended to exclude cysts, which do not form in the type species, S. hydrophila. Afenestrata africana differs from S. hydrophila by the formation of cysts, the dorsal position of the anus in females, the shorter stylet, and a pore-like phasmid opening in second-stage juveniles. In addition, the lip pattern of males and juveniles is characterized by a greater degree of fusion of lip parts, the host response is a syncytium (versus a single uninucleate giant cell in S. hydrophila), and the cuticle is thinner and lacks a D layer. Unlike Heterodera, the cyst of Afenestrata lacks fenestrae. PMID:19294081

  15. Characterization of a protein tyrosine phosphatase gene CvBV202 from Cotesia vestalis polydnavirus (CvBV).

    PubMed

    Shi, Min; Chen, Ya-Feng; Yao, Yuan; Huang, Fang; Chen, Xue-Xin

    2008-06-01

    Cotesia vestalis (Haliday) (Hymenoptera: Braconidae) is an endoparasitoid of the larval stage of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae) and injects a polydnavirus (CvBV) into its host during oviposition. In this paper we characterize CvBV202 and its product. CvBV202 is located on segment S2 of CvBV genome; it has a size of 984 bp and encodes a putative protein of 328 amino acids, including protein phosphatase domain and tyrosine-specific protein phosphatase domain. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected for 6 days. Tissue-specific patterns of this gene expression showed that CvBV202 had a close relationship with the host's physiological alternations including immunosuppression, modulation of hormone titer, and nutrition metabolism. The protein was detected in the parasitized hosts at 12 h p.p. using western blot assay. The product of CvBV202 was found to be around 59 kDa, much larger than the predicted molecular weight of 37.8 kDa, suggesting that post-translational modification of CvBV202 occurs in host cells, corresponding with the existence of many post-translational modification sites. Immunofluorescence staining and confocal laser scanning microscopy revealed that CvBV202 and the fused protein eGFP-CvBV202 were observed both in the nuclear region and cytoplasm of the hemocytes of the naturally parasitized host larvae and rBac-eGFP-CvBV202-infected Tn-5B1-4 cells, respectively. PMID:18425572

  16. Micropropagation of sour cherry ( Prunus cerasus L.) cv. Šumadinka

    Microsoft Academic Search

    Radosav Cerovi?; Djurdina Ruži?

    1987-01-01

    Sour cherry cv. Šumadinka (Prunus cerasus L.) is the leading Yugoslav cultivar for production orchards. A method of micropropagation has been developed for the purpose of growing ‘Šumadinka’ on its own roots and for rapid multiplication.

  17. Axel Jantsch -Complete CV 1 Personal facts 2

    E-print Network

    Jantsch, Axel

    Axel Jantsch - Complete CV June 2013 Contents 1 Personal facts 2 2 Research interests 2 3 Education.3 Leadership in research and education programs . . . . . . . . . . . . . . . . . . 4 4.4 Industrial positions Education 16 9.1 Course responsible . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 9

  18. Jason Aughenbaugh: CV Page 1 JASON MATTHEW AUGHENBAUGH

    E-print Network

    Jason Aughenbaugh: CV Page 1 JASON MATTHEW AUGHENBAUGH CURRICULUM VITAE SUMMARY OF INTERESTS applications in reliability analysis of dynamic systems, risk-based design, simulation- based design, sensor.D. 2006 Mechanical Engineering Georgia Institute of Technology Dissertation: Managing uncertainty

  19. Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae

    E-print Network

    Reber, Paul J.

    Finkel CV, Page 1 Eli J. Finkel ­ Curriculum Vitae (November, 2014) Professional Information Mail://faculty.wcas.northwestern.edu/ http://www.kellogg.northwestern.edu/ eli-finkel/ faculty/directory/ finkel_eli.aspx Education Ph

  20. Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae

    E-print Network

    Reber, Paul J.

    Finkel CV, Page 1 Eli J. Finkel ­ Curriculum Vitae (July, 2014) Professional Information Mail://faculty.wcas.northwestern.edu/ http://www.kellogg.northwestern.edu/ eli-finkel/ faculty/directory/ finkel_eli.aspx Education Ph

  1. van de Gevel -CV1 SASKIA L. VAN DE GEVEL

    E-print Network

    Thaxton, Christopher S.

    of Costa Rica, Senior Seminar Capstone Course, Environmental Issues in Appalachia Course - Biogeography of Costa Rica, Environmental Issues in Appalachia, Introduction to the Natural 2005 Environment: Weather, Climate, and Biomes. #12;van de Gevel - CV2 Head

  2. ASAS-SN Discovery of a Likely CV

    NASA Astrophysics Data System (ADS)

    Davis, A. B.; Shappee, Bart; Shappee, B. J.; Will, D.; Simonian, G.; Stanek, K. Z.; Holoien, T. W.-S.; Kochanek, C. S.; Basu, U.; Gross, N.; Beacom, J. F.; Prieto, J. L.; Bersier, D.; Brimacombe, J.; Dong, Subo; Wozniak, P. R.; Szczygiel, D.; Pojmanski, G.

    2014-12-01

    During the ongoing All Sky Automated Survey for SuperNovae (ASAS-SN or "Assassin"), using data from the quadruple 14-cm "Brutus" telescope in Haleakala, Hawaii, we discovered a new transient source, likely a CV.

  3. Elephant (Loxodonta africana) Home Ranges in Sabi Sand Reserve and Kruger National Park: A Five-Year Satellite Tracking Study

    PubMed Central

    Thomas, Bindi; Holland, John D.; Minot, Edward O.

    2008-01-01

    During a five-year GPS satellite tracking study in Sabi Sand Reserve (SSR) and Kruger National Park (KNP) we monitored the daily movements of an elephant cow (Loxodonta africana) from September 2003 to August 2008. The study animal was confirmed to be part of a group of seven elephants therefore her position is representative of the matriarchal group. We found that the study animal did not use habitat randomly and confirmed strong seasonal fidelity to its summer and winter five-year home ranges. The cow's summer home range was in KNP in an area more than four times that of her SSR winter home range. She exhibited clear park habitation with up to three visits per year travelling via a well-defined northern or southern corridor. There was a positive correlation between the daily distance the elephant walked and minimum daily temperature and the elephant was significantly closer to rivers and artificial waterholes than would be expected if it were moving randomly in KNP and SSR. Transect lines established through the home ranges were surveyed to further understand the fine scale of the landscape and vegetation representative of the home ranges. PMID:19065264

  4. Phoretic interaction between the kangaroo leech Marsupiobdella africana (Hirudinea: Glossiphoniidae) and the cape river crab Potamonautes perlatus (Decapoda: Potamonautidae)?

    PubMed Central

    Badets, Mathieu; Preez, Louis Du

    2013-01-01

    The South African leech Marsupiobdella africana is a temporary ectoparasite of the amphibian Xenopus laevis, has a phoretic association with a freshwater crab Potamonautes perlatus, and exhibits advanced parental care by incubating its offspring in a brood pouch. Because phoretic associations are usually regarded to favor the phoront’s dispersion, its occurrence within the biology of a parasitic species reflects an intimate context of interactions. In addition to phoresy, attachment to the crab may confer other advantages pertaining to offspring development and predator avoidance, dispersion and the parasitic life cycle. Two ponds where amphibian and crab hosts co-occur were sampled twice a month for a period of 1 year. The population dynamics of the leeches and their use of specific microhabitats as attachment sites on the crabs were also investigated. Results indicate a direct relationship between intra-specific variation in the sex ratio among captured crab hosts and the number of leeches recruited over time. The attachments to specific microhabitats on the hard surfaces of the host suggest a proximal proximate anti-predatory strategy. Finally, the importance of oxygen accessibility for the offspring development has been investigated experimentally. Results revealed a remarkable network of interactions linking all partners of this system raising the question as to whether the crabs merely act as a vehicle or play a role within the parasitic life cycle. PMID:24918071

  5. Characterization of a Novel Polyomavirus Isolated from a Fibroma on the Trunk of an African Elephant (Loxodonta africana)

    PubMed Central

    Stevens, Hans; Bertelsen, Mads Frost; Sijmons, Steven; Van Ranst, Marc; Maes, Piet

    2013-01-01

    Viruses of the family Polyomaviridae infect a wide variety of avian and mammalian hosts with a broad spectrum of outcomes including asymptomatic infection, acute systemic disease, and tumor induction. In this study a novel polyomavirus, the African elephant polyomavirus 1 (AelPyV-1) found in a protruding hyperplastic fibrous lesion on the trunk of an African elephant (Loxodonta africana) was characterized. The AelPyV-1 genome is 5722 bp in size and is one of the largest polyomaviruses characterized to date. Analysis of the AelPyV-1 genome reveals five putative open-reading frames coding for the classic small and large T antigens in the early region, and the VP1, VP2 and VP3 capsid proteins in the late region. In the area preceding the VP2 start codon three putative open-reading frames, possibly coding for an agnoprotein, could be localized. A regulatory, non-coding region separates the 2 coding regions. Unique for polyomaviruses is the presence of a second 854 bp long non-coding region between the end of the early region and the end of the late region. Based on maximum likelihood phylogenetic analyses of the large T antigen of the AelPyV-1 and 61 other polyomavirus sequences, AelPyV-1 clusters within a heterogeneous group of polyomaviruses that have been isolated from bats, new world primates and rodents. PMID:24205012

  6. Activation and inhibition of thermosensitive TRP channels by voacangine, an alkaloid present in Voacanga africana, an African tree.

    PubMed

    Terada, Yuko; Horie, Syunji; Takayama, Hiromitsu; Uchida, Kunitoshi; Tominaga, Makoto; Watanabe, Tatsuo

    2014-02-28

    Voacangine (1) is an alkaloid found in the root bark of Voacanga africana. Our previous work has suggested that 1 is a novel transient receptor potential vanilloid type 1 (TRPV1) antagonist. In this study, the agonist and antagonist activities of 1 were examined against thermosensitive TRP channels. Channel activity was evaluated mainly using TRP channel-expressing HEK cells and calcium imaging. Herein, it was shown that 1 acts as an antagonist for TRPV1 and TRPM8 but as an agonist for TRPA1 (EC50, 8 ?M). The compound competitively blocked capsaicin binding to TRPV1 (IC50, 50 ?M). Voacangine (1) competitively inhibited the binding of menthol to TRPM8 (IC50, 9 ?M), but it showed noncompetitive inhibition against icilin (IC50, 7 ?M). Moreover, the compound selectively abrogated chemical agonist-induced TRPM8 activation and did not affect cold-induced activation. Among these effects, the TRPM8 inhibition profile is unique and noteworthy, because to date no studies have reported a menthol competitive inhibitor of TRPM8 derived from a natural source. Furthermore, this is the first report of a stimulus-selective TRPM8 antagonist. Accordingly, 1 may contribute to the development of a novel class of stimulus-selective TRPM8 blockers. PMID:24484240

  7. Elephant (Loxodonta africana) home ranges in Sabi Sand Reserve and Kruger National Park: a five-year satellite tracking study.

    PubMed

    Thomas, Bindi; Holland, John D; Minot, Edward O

    2008-01-01

    During a five-year GPS satellite tracking study in Sabi Sand Reserve (SSR) and Kruger National Park (KNP) we monitored the daily movements of an elephant cow (Loxodonta africana) from September 2003 to August 2008. The study animal was confirmed to be part of a group of seven elephants therefore her position is representative of the matriarchal group. We found that the study animal did not use habitat randomly and confirmed strong seasonal fidelity to its summer and winter five-year home ranges. The cow's summer home range was in KNP in an area more than four times that of her SSR winter home range. She exhibited clear park habitation with up to three visits per year travelling via a well-defined northern or southern corridor. There was a positive correlation between the daily distance the elephant walked and minimum daily temperature and the elephant was significantly closer to rivers and artificial waterholes than would be expected if it were moving randomly in KNP and SSR. Transect lines established through the home ranges were surveyed to further understand the fine scale of the landscape and vegetation representative of the home ranges. PMID:19065264

  8. Insights from High Precision Oxygen Isotopic Analyses of Reduced CV Meteorite Separates: CV Matrix Homogeneity and Preservation of Nebular Heterogeneity

    NASA Astrophysics Data System (ADS)

    Dyl, K. A.; Young, E. D.

    2011-03-01

    The oxygen-isotopic composition of matrix from reduced CVs Efremovka and Vigarano has been measured and is indistinguishable from Allende. This indicates a similar nebular history and a preservation of different nebular conditions within reduced CV meteorites.

  9. Ch OpenCV for interactive open architecture computer vision Qingcang Yua

    E-print Network

    Cheng, Harry H.

    Ch OpenCV for interactive open architecture computer vision Qingcang Yua , Harry H. Chenga,*, Wayne and implementation of an interactive open architecture computer vision software package called Ch OpenCV is presented. Benefiting from both Ch and OpenCV, Ch OpenCV has many salient features. It is interactive, capable

  10. The Social and Ecological Integration of Captive-Raised Adolescent Male African Elephants (Loxodonta africana) into a Wild Population

    PubMed Central

    Evans, Kate; Moore, Randall; Harris, Stephen

    2013-01-01

    Background A rapid rise in the number of captive African elephants (Loxodonta africana) used in the tourism industry in southern Africa and orphaned elephants in human care has led to concerns about their long-term management, particularly males. One solution is to release them into the wild at adolescence, when young males naturally leave their herd. However, this raises significant welfare concerns: little is known about how well released elephants integrate into wild populations and whether they pose a greater threat to humans than wild elephants. We document the release of three captive-raised adolescent male African elephants in the Okavango Delta, Botswana. Methodology/Principal Findings Despite having been part of a herd of working elephants for at least eight years, the three males progressively integrated into the complex fission-fusion society of wild bull elephants. In the three years following release, they showed no tendency to be closer to human habitation, and there were no significant differences between wild and captive-raised adolescent males in the total number of social interactions, size of ranges and habitat use. However, the captive-raised elephants sparred less and vocalised more, and spent more time alone and in smaller social groups. Thereafter the released elephants continued to expand their ranges and interact with both mixed-sex herds and males. One male was shot by farmers 94 months after release, along with ten wild elephants, on a ranch outside the protected area. Conclusions/Significance We show that captive-raised adolescent male elephants can integrate into a wild population. Long-term studies are required to determine the longevity, breeding success, and eventual fate of released male elephants, but we identified no significant short-term welfare problems for the released elephants or recipient population. Release of captive-raised mammals with complex social systems is a husbandry option that should be explored further. PMID:23437076

  11. Opaque Assemblages in CK and CV Carbonaceous Chondrites

    NASA Technical Reports Server (NTRS)

    Neff, K. E.; Righter, K.

    2006-01-01

    CK carbonaceous chondrites are the only group of carbonaceous chondrites that exhibit thermal metamorphism. As a result, CKs display features of metamorphism such as silicate darkening, recrystallization and shock veins. Calcium Aluminum Inclusions and Fe-Ni metal are rare. CV carbonaceous chondrites are unequilibrated and have two subgroups; oxidized and reduced. The CV and CK carbonaceous chondrite groups have been compared to each other often because of petrographic similarities, such as overlapping oxygen isotopic ratios. Scientists have suggested the two groups of carbonaceous chondrites formed from the same parent body and CKs are equilibrated CV chondrites [1, 2]. The oxidized CV group has been most closely related to CKs. This study examines the petrology and mineralogy of CKs and CVs focusing on opaque minerals found in the meteorites. Using the oxide, metal and sulfide assemblages, constraints can be placed on the temperature and oxygen fugacity at which the meteorites equilibrated. The temperature and oxygen fugacity of the CK and CV chondrites can be compared in order to help define their formation history.

  12. Endothelium/Nitric Oxide Mediates the Vasorelaxant and Antihypertensive Effects of the Aqueous Extract from the Stem Bark of Mammea africana Sabine (Guttiferae)

    PubMed Central

    Nguelefack-Mbuyo, Elvine Pami; Dongmo, Alain Bertrand; Nguelefack, Télesphore Benoît; Kamanyi, Albert; Kamtchouing, Pierre; Dimo, Théophile

    2012-01-01

    This study evaluates the vasorelaxant and antihypertensive effects of the aqueous extract from the stem bark of M. africana (AEMA). AEMA was tested in vitro on intact or endothelium-denuded rats' aorta rings precontracted with KCl or norepinephrine in absence or in presence of L-NAME or glibenclamide. The effect of a single concentration (300??g/mL) of AEMA was also examined on the concentration-response curve of KCl. In vivo, the antihypertensive effects of AEMA (200?mg/kg/day) were evaluated in male Wistar rats treated with L-NAME (40?mg/kg/day) for 4 weeks. AEMA relaxed aorta rings precontracted with NE or KCl with respective EC50 values of 0.36??g/mL and 197.60??g/mL. The destruction of endothelium or pretreatment of aorta rings with L-NAME shifted the EC50 of AEMA from 0.36??g/mL to 40.65??g/mL and 20.20??g/mL, respectively. The vasorelaxant activity of M. africana was significantly inhibited in presence of glibenclamide. AEMA also significantly inhibited the concentration-response curve of KCl. Administered orally, AEMA induced acute and chronic antihypertensive effects and normalized renal NO level. These results show that the vasorelaxant activity of AEMA might be mediated by the activation of the NO-cGMP-ATP-dependent potassium channels pathway and might predominantly account for its antihypertensive effect. PMID:23008745

  13. Development of the Micromonospora carbonacea var. africana ATCC 39149 bacteriophage pMLP1 integrase for site-specific integration in Micromonospora spp.

    PubMed

    Alexander, Dylan C; Devlin, David J; Hewitt, Duane D; Horan, Ann C; Hosted, Thomas J

    2003-09-01

    Micromonospora carbonacea var. africana ATCC 39149 contains a temperate bacteriophage, pMLP1, that is present both as a replicative element and integrated into the chromosome. Sequence analysis of a 4.4 kb KpnI fragment revealed pMLP1 att/int functions consisting of an integrase, an excisionase and the phage attachment site (attP). Plasmids pSPRH840 and pSPRH910, containing the pMLP1 att/int region, were introduced into Micromonospora spp. by conjugation from Escherichia coli. Sequence analysis of DNA flanking the integration site confirmed site-specific integration into a tRNAHis gene in the chromosome. The pMLP1 attP element and chromosomal bacterial attachment (attB) site contain a 24 bp region of sequence identity located at the 3' end of the tRNA. Integration of pMLP1-based plasmids in M. carbonacea var. africana caused a loss of the pMLP1 phage. Placement of an additional attB site into the chromosome allowed integration of pSPRH840 into the alternate attB site. Plasmids containing the site-specific att/int functions of pMLP1 can be used to integrate genes into the chromosome. PMID:12949170

  14. Effect of UV irradiation, toluidine blue, and environment on maternal haploid frequencies from the cross between Nicotiana tabacum and N. africana

    SciTech Connect

    Chimoyo, H.M.

    1988-01-01

    Treating Nicotiana africana Merxm. pollen with three levels UV radiation prior to pollinating four cultivars of flue-cured tobacco (Coker 176, NC95, McN944 and PD4), Nicotiana tabacum produced 1,953 viable seedlings from an estimated total of 170,248 seeds, of which 1,667 were haploid and 286 were hybrids. Drenching N. tabacum flowers with toluidine blue 18 hours after pollination with normal N. africana pollen, yielded 511 viable seedlings from 70,613 seeds, of which 346 were haploid and 165 hybrids. Untreated pollen gave 548 viable seedlings from 56,291 seeds, comprising 341 haploids and 208 hybrids. Contrary to results from a previous histological study, in vivo pollen tube growth rate appears to be similar irrespective of pollen source or treatment, and fertilization seems to occur at about the same time as in the selfed control. From an estimated total of 803,854 seeds sown, 3,014 viable seedlings were obtained. Coker 176 gave significantly higher yields of haploids than the other three cultivars. Field grown plants produced more haploids than greenhouse grown plants. Further evidence was obtained to support selective chromosomal elimination as the mechanism governing the development of maternal haploids from this interspecific cross.

  15. CV of BIN LIU Page 1 of 5

    E-print Network

    Parker, Matthew D. Brown

    CV of BIN LIU Page 1 of 5 Curriculum Vitae BIN LIU, Ph.D. CURRENT AFFILIATION Research Assistant-12. 5. Li, S., D. Zhao, L. Zhou, and B. Liu, 2013: Dependence of mean square slope on wave state and its. Liu, B., C. Guan, L. Xie, and D. Zhao, 2012: An Investigation of the effects of wave state and sea

  16. R. MICHAEL YOUNG CV MAY 2014 R. Michael Young

    E-print Network

    Young, R. Michael

    !1 R. MICHAEL YOUNG CV MAY 2014 R. Michael Young Professor Department of Computer Science Campus://liquidnarrative.csc.ncsu.edu/rmy/ ! BIOGRAPHIC SUMMARY ! Michael Young is a Professor of Computer Science at North Carolina State University was among the first researchers to develop models of planning adapted for story generation and leveraged

  17. Computational simulation of CV combination preferences in babbling

    PubMed Central

    Nam, Hosung; Goldstein, Louis M.; Giulivi, Sara; Levitt, Andrea G.; Whalen, D. H.

    2013-01-01

    There is a tendency for spoken consonant-vowel (CV) syllables, in babbling in particular, to show preferred combinations: labial consonants with central vowels, alveolars with front, and velars with back. This pattern was first described by MacNeilage and Davis, who found the evidence compatible with their “frame-then-content” (F/C) model. F/C postulates that CV syllables in babbling are produced with no control of the tongue (and therefore effectively random tongue positions) but systematic oscillation of the jaw. Articulatory Phonology (AP; Browman & Goldstein) predicts that CV preferences will depend on the degree of synergy of tongue movements for the C and V. We present computational modeling of both accounts using articulatory synthesis. Simulations found better correlations between patterns in babbling and the AP account than with the F/C model. These results indicate that the underlying assumptions of the F/C model are not supported and that the AP account provides a better and account with broader coverage by showing that articulatory synergies influence all CV syllables, not just the most common ones. PMID:24496111

  18. A Charge Balanced CV Converter for a Differential Capacitance Sensor

    Microsoft Academic Search

    Masahiro Tsugai; Yoshiaki Hirata; Toru Araki; Masafumi Kimata

    2003-01-01

    This paper presents a simple new configuration for a switched capacitor type C-V converter based on a charge balance principle for a differential capacitance sensor and its simulation results. The new converter was devised and realized using only one operational amplifier, nine CMOS switches and an oscillation circuit. It was shown that the offset output due to the input offset

  19. HARVARDgazette04-17 FEBRUARY 2010 VOL. CV NO. 9

    E-print Network

    HARVARDgazette04-17 FEBRUARY 2010 VOL. CV NO. 9 NEWS.HARVARD.EDU/GAZETTE Athletics for all Cambridge, MA 02138 Periodical postage paid at Boston, MA. Harvard Gazette (issn: 0364-7692) is published Square before being lovingly roasted inside New College Theatre. Man of the Year Justin Timberlake

  20. Effects of paclobutrazol on avocado (Persea americana Mill.) cv. ‘Fuerte’

    Microsoft Academic Search

    I. Adato

    1990-01-01

    Adato, I., 1990. Effects of paclobutrazol on avocado (Persea americana Mill. ) cv. 'Fuerte'. Scientia Hortic., 45:105-115. Mature avocado trees (Persea americana Mill. ) of cultivar 'Fuerte' were sprayed with Cultar ~, a commercial formulation containing 25% paclobutrazol (ICI, U.K.). Hand spraying of individual trees before or at the flowering stage resulted in an increase in the number of harvested

  1. Finkel CV, Page 1 Eli J. Finkel Curriculum Vitae

    E-print Network

    Reber, Paul J.

    Finkel CV, Page 1 Eli J. Finkel ­ Curriculum Vitae (October, 2013) Professional Information Mail@kellogg.northwestern.edu Numbers: Phone: 847-491-3212 / Fax: 847-491-7859 TBD Psych Website: faculty.wcas.northwestern.edu/eli-finkel/ MORS Website: http://www.kellogg.northwestern.edu/faculty/directory/finkel_eli.aspx Education Ph

  2. CV, T.S. Hopkins, 2009 Thomas Sawyer HOPKINS

    E-print Network

    Parker, Matthew D. Brown

    CV, T.S. Hopkins, 2009 1 Thomas Sawyer HOPKINS 288 Jefferson Rd, Princeton, NJ, USA Date of Birth 27695 Home: 288 Jefferson Rd. Princeton NJ 08540, USA email: tom_hopkins@ncsu.edu CNR/IAMC - Institute 3811, 3817 Fax:+39 081-542 3888 email: tom.hopkins@iamc.cnr.it EDUCATION Degree Institution Conferring

  3. Segmentation of kidney using CV model and anatomy priors

    Microsoft Academic Search

    Jinghua Lu; Jie Chen; Juan Zhang; Wenjia Yang

    2007-01-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and

  4. Visitor face tracking system using OpenCV library

    Microsoft Academic Search

    Yong Kok Ching; A. S. Prabuwono; R. Sulaiman

    2009-01-01

    This paper presents the development of visitor face tracking system using OpenCV library. This system can be used for security purpose to record the visitor face. The user can identify who had been visiting him\\/her when away from the place. Visitors just need to appear in front of the web camera for at least 10 second, their image will be

  5. The Implementation of Lane Detective Based on OpenCV

    Microsoft Academic Search

    Wu Ye; Shan Yuetian; Xu Yunhe; Wang Shu; Zhuang Yuchen

    2010-01-01

    In intelligent vehicle systems, lane detection is one of the most important parts. This paper presents a lane detection algorithm that based on Hough transform. Principle of the algorithm and the implementation base on OpenCV are discussed in detail. The algorithm was verified at the end of this paper.

  6. Properties of NAC and CV[NAC] for Energy Models

    E-print Network

    Ruch, D. K.

    1993-01-01

    ESL-TR-93/05-03 Properties of CV [NAC] for Linear Energy Models David K. Ruch Sam Houston State University k Energy Systems Laboratory May 1993 1. Introduction and Notation The stability of the NAC estimate for the PRISM model has been shown em...

  7. C. Atsriku_CV1 Christian Atsriku (Ph.D)

    E-print Network

    Lawson, Catherine L.

    OF EXPERTISE: Drug metabolism Studies for small molecules: Metabolite profiling and identification for cold and quantification of post-translational modifications, protein enrichment and purification techniques Cell Cultures (1987-1991): University of Science and Technology, Kumasi Ghana #12;C. Atsriku_CV2 EMPLOYMENT

  8. Recurrence of hyperprolactinemia and continuation of ovarian acyclicity in captive African elephants (Loxodonta africana) treated with cabergoline.

    PubMed

    Morfeld, Kari A; Ball, Ray L; Brown, Janine L

    2014-09-01

    Hyperprolactinemia is associated with reproductive acyclicity in zoo African elephants (Loxodonta africana) and may contribute to the non-self-sustainability of the captive population in North America. It is a common cause of infertility in women and other mammals and can be treated with the dopamine agonist cabergoline. The objectives of this study were to assess prolactin responses to cabergoline treatment in hyperprolactinemic, acyclic African elephants and to determine the subsequent impact on ovarian cyclic activity. Five elephants, diagnosed as hyperprolactinemic (>11 ng/ml prolactin) and acyclic (maintenance of baseline progestagens for at least 1 yr), were treated with 1-2 mg cabergoline orally twice weekly for 16-82 wk. Cabergoline reduced (P < 0.05) serum prolactin concentrations during the treatment period compared to pretreatment levels in four of five elephants (11.5 +/- 3.2 vs. 9.1 +/- 3.4 ng/ml; 20.3 +/- 16.7 vs. 7.9 +/- 9.8 ng/ml; 26.4 +/- 15.0 vs. 6.8 +/- 1.5 ng/ml; 42.2 +/- 22.6 vs. 18.6 +/- 8.9 ng/ml). However, none of the females resumed ovarian cyclicity based on serum progestagen analyses up to 1 yr posttreatment. In addition, within 1 to 6 wk after cessation of oral cabergoline, serum prolactin concentrations returned to concentrations that were as high as or higher than before treatment (P < 0.05). One elephant that exhibited the highest pretreatment prolactin concentration (75.2 +/- 10.5 ng/ml) did not respond to cabergoline and maintained elevated levels throughout the study. Thus, oral cabergoline administration reduced prolactin concentrations in elephants with hyperprolactinemia, but there was no resumption of ovarian cyclicity, and a significant prolactin rebound effect was observed. It is possible that higher doses or longer treatment intervals may be required for cabergoline treatment to result in permanent suppression of prolactin secretion and to mitigate associated ovarian cycle problems. PMID:25314824

  9. GpuCV: an opensource GPU-accelerated framework forimage processing and computer vision

    Microsoft Academic Search

    Yannick Allusse; Patrick Horain; Ankit Agarwal; Cindula Saipriyadarshan

    2008-01-01

    This paper presents GpuCV, an open source multi-platform library for easily developing GPU-accelerated image processing and Computer Vision operators and applications. It is meant for computer vision scientist not familiar with GPU technologies. It is designed to be compatible with Intel's OpenCV library by offering GPU-accelerated operators that can be integrated into native OpenCV applications. The GpuCV framework transparently manages

  10. An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection

    E-print Network

    Rose, Jonathan

    An Energy Efficient FPGA Hardware Architecture for the Acceleration of OpenCV Object Detection for the Acceleration of OpenCV Object Detection Braiden Brousseau Master of Applied Science Graduate Department . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.4 OpenCV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.5 Multi

  11. An Energy-Efficient, Fast FPGA Hardware Architecture for OpenCV-Compatible Object

    E-print Network

    Rose, Jonathan

    An Energy-Efficient, Fast FPGA Hardware Architecture for OpenCV-Compatible Object Detection Braiden source OpenCV computer vision software. The architecture is novel, compared to prior work in this area Computer Vision project, OpenCV [3]. We should note that the mobile context of this paper rests

  12. Properties of a Cationic Peroxidase from Citrus jambhiri cv. Adalia

    Microsoft Academic Search

    Saleh A. Mohamed; Mohamed O. El-Badry; Ehab A. Drees; Afaf S. Fahmy

    2008-01-01

    The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the\\u000a juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes\\u000a from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatography on diethylaminoethanol-cellulose,

  13. A camera calibration technique based on OpenCV

    Microsoft Academic Search

    Y. M. Wang; Y. Li; J. B. Zheng

    2010-01-01

    Camera calibration is very improtant in 3D computer vision systems. In this paper, the camera model in OpenCV (open source computer vision library) is discussed, the non-linear distortion of the tangential and radial distortion aberration are considered. Based on these, we introduce the corner extraction of the Camera Calibration Toolbox for Matlab, and an arithmetic of camera calibration based on

  14. Africana Studies Film Studies

    E-print Network

    CENTER EVENT OVAL Anthropology Dance Economics Human Rights Studies Physical Education Political Science COURTYARD LEHMAN LAWN Architecture Art History Classics & Ancient Studies Music Philosophy Religion DIANA Computer Science Environmental Science Mathematics Physics & Astronomy Statistics In the event of rain

  15. [On "the moxibustion shouldn't be applied at Shimen (CV 5) on female"].

    PubMed

    Xuan, Da-Ke; Wu, Gui-Hua

    2013-05-01

    The TCM theory "moxibustion shouldn't be applied at Shimen (CV 5) on female" is discussed in this article, which provides theoretical instruction on proper clinical application of Shimen (CV 5). Based on literature study, ancient classics concerning contraindication of "moxibustion shouldn't be applied at Shimen (CV 5) on female" were studied. TCM theory "moxibustion shouldn't be applied at Shimen (CV 5) on female" was explored profoundly through ancient record, physiological relations between Shimen (CV 5) and pregnancy, the relation between the anatomic structure of Shimen (CV 5) and the extra acupoint Jueyun Xue. And the result indicated that as an acupoint with contraindications, Shimen (CV 5) should be attached with great importance in its clinical practice on female patients. Reinforcing manipulation should be given instead of reducing. Moxibustion should be applied with cautiousness on the point, especially during pregnancy. PMID:23885628

  16. Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China.

    PubMed

    Zhang, Zhicheng; Dai, Wei; Wang, Shaohui; Dai, Dingzhen

    2015-01-01

    Pigeon circovirus (PiCV) is one of four viruses in the family Circoviridae that affect young pigeons around the world. We collected 158 serum or tissue samples from six poultry farms in eastern China to investigate the prevalence and genetic characteristics of PiCV in Chinese pigeons. We tested for PiCV using a PCR assay and found that PiCV was present in 80.7 % (88/109) of diseased pigeons and 63.3 % (31/49) of healthy pigeons; overall, 75.3 % (119/158) of samples were PiCV positive. One PiCV-positive sample from each poultry farm was randomly chosen for amplification of the complete PiCV genome by inverse primer PCR (IP-PCR). The six genomic PiCV strains were designated as AHBZ (KJ704801), HBLF-E2 (KJ704802), JSJN (KJ704803), NJPK-21 (KJ704804), SDDZ (KJ704805) and SHWH-AB4 (KJ704806). We compared these new PiCV genomes to six publicly available PiCV genomes and found that the Rep and Cap genes had sequence identity ranging from 93.8 % to 100 % and 79.1 % to 100 %, respectively. In a phylogenetic analysis, PiCV and eight other members of the genus Circovirus were sister to chicken anemia virus (CAV), the only member of genus Gyrovirus. The results of this study provide evidence that PiCV is present in Chinese pigeons at a high rate and that PiCV is a viral lineage that is distinct from CAV. PMID:25348272

  17. Odour-active compounds in papaya fruit cv. Red Maradol.

    PubMed

    Pino, Jorge A

    2014-03-01

    Application of solid-phase microextraction and simultaneous distillation-extraction combined with GC-FID, GC-MS, aroma extract dilution analysis, and odour activity value were used to analyse volatile compounds from papaya fruit cv. Red Maradol and to estimate the most odour-active compounds. The analyses led to the identification of 137 compounds; 118 of them were positively identified. Twenty-five odorants were considered as odour-active compounds and contribute to the typical papaya aroma, from which ethyl butanoate, benzyl isothiocyanate, 1-hexen-3-one, (E)-?-ionone, and methyl benzoate were the most odour-active compounds. PMID:24176322

  18. Magnetic CVs in the UCT CCD CV Survey

    E-print Network

    Woudt, P A; Woudt, Patrick A.; Warner, Brian

    2003-01-01

    An overview is given of all the magnetic CVs found in the UCT CCD CV Survey (Woudt & Warner 2001, 2002, 2003a). We have identified eight new candidate Intermediate Polars (IP), of which six are classical novae (RR Cha, DD Cir, AP Cru, V697 Sco, V373 Sct, and RX J1039.7-0507). The two other candidate IPs are Aqr1 (2236+0052) and RX J0944.5+0357. In addition, there are two probable Polars, namely V351 Pup (= Nova Puppis 1991) and FIRST J102347.6+003841.

  19. No nebular magnetization in the Allende CV carbonaceous chondrite

    NASA Astrophysics Data System (ADS)

    Fu, R. R.; Lima, E. A.; Weiss, B. P.

    2014-10-01

    Magnetic fields in the solar nebula may have played a central role in mass and angular momentum transport in the protosolar disk and facilitated the accretion of the first planetesimals. Thought to be key evidence for this hypothesis is the high unblocking-temperature, randomly oriented magnetization in chondrules in the Allende CV carbonaceous chondrite. However, it has recently been realized that most of the ferromagnetic minerals in Allende are products of secondary processes on the parent planetesimal. Here we reevaluate the pre-accretional magnetism hypothesis for Allende using new paleomagnetic analyses of chondrules including the first measurements of mutually oriented subsamples from within individual chondrules. We confirm that Allende chondrules carry a high-temperature component of magnetization that is randomly oriented among chondrules. However, we find that subsamples of individual chondrules are also non-unidirectionally magnetized. Therefore, the high-temperature magnetization in Allende chondrules is not a record of nebular magnetic fields and is instead best explained by remagnetization during metasomatism in a <8 ?T magnetic field. This low field intensity suggests that any core dynamo on the CV parent body decayed before the end of metasomatism, likely <40 My after the formation of calcium aluminum-rich inclusions (CAIs). Despite widespread practice, the magnetization in Allende should not be used to constrain magnetic fields in the protosolar nebula.

  20. The All-Sky Automated Survey for Supernovae CV Patrol

    NASA Astrophysics Data System (ADS)

    Davis, Alexandra Bianca; Shappee, Benjamin John; Archer Shappee, Bartlett; ASAS-SN

    2015-01-01

    Even in the modern era, only human eyes scan the entire optical sky for the violent, variable, and transient events that shape our universe. The "All Sky Automated Survey for Supernovae" (ASAS-SN or "Assassin") is changing this by monitoring the extra-galactic sky down to V~17 mag every 2-3 days using multiple telescopes, hosted by Las Cumbres Observatory Global Telescope Network, in the northern and southern hemispheres. By far the most common events observed by ASAS-SN are the Galactic transients. Since April 2013 ASAS-SN has identified over 180 new cataclysmic variable stars and announced over 260 new outbursts of known CVs. To make our data available to the CV community in 'real time', we have launched an automated 'CV Patrol' to monitor known CVs for outbursts as a useful tool for both professional and amateurs astronomers. It is a long term goal of ASAS-SN to make all our data public in real-time, and this patrol will serve as a framework for future ASAS-SN data releases.

  1. Antimicrobial activity and probable mechanisms of action of medicinal plants of Kenya: Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus.

    PubMed

    Mwitari, Peter G; Ayeka, Peter A; Ondicho, Joyce; Matu, Esther N; Bii, Christine C

    2013-01-01

    Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus are used traditionally in Kenya for treatment of microbial infections and cancer. Information on their use is available, but scientific data on their bioactivity, safety and mechanisms of action is still scanty. A study was conducted on the effect of organic extracts of these plants on both bacterial and fungal strains, and their mechanisms of action. Extracts were evaluated through the disc diffusion assay. Bacteria and yeast test strains were cultured on Mueller-Hinton agar and on Sabouraud dextrose agar for the filamentous fungi. A 0.5 McFarland standard suspension was prepared. Sterile paper discs 6 mm in diameter impregnated with 10 µl of the test extract (100 mg/ml) were aseptically placed onto the surface of the inoculated media. Chloramphenicol (30 µg) and fluconazole (25 µg) were used as standards. Discs impregnated with dissolution medium were used as controls. Activity of the extracts was expressed according to zone of inhibition diameter. MIC was determined at 0.78-100 mg/ml. Safety studies were carried using Cell Counting Kit 8 cell proliferation assay protocol. To evaluate extracts mechanisms of action, IEC-6 cells and RT-PCR technique was employed in vitro to evaluate Interleukin 7 cytokine. Investigated plants extracts have both bactericidal and fungicidal activity. W. ugandensis is cytotoxic at IC50<50 µg/ml with MIC values of less than 0.78 mg/ml. Prunus africana shuts down expression of IL 7 mRNA at 50 µg/ml. W. somnifera has the best antimicrobial (1.5625 mg/ml), immunopotentiation (2 times IL 7 mRNA expression) and safety level (IC50>200 µg/ml). Fractions from W. ugandensis and W. somnifera too demonstrated antimicrobial activity. Mechanisms of action can largely be attributed to cytotoxicity, Gene silencing and immunopotentiation. Use of medicinal plants in traditional medicine has been justified and possible mechanisms of action demonstrated. Studies to isolate and characterize the bioactive constituents continue. PMID:23785437

  2. Fabric defect detection based on open source computer vision library OpenCV

    Microsoft Academic Search

    Xiaojun Jia

    2010-01-01

    A method for fabric defect detection based on OpenCV with rich computer vision and image processing algorithms and functions is presented. Firstly, OpenCV image processing functions implement fabric image preprocessing. We use morphological opening and closing operations to segment image because of their blur defects. Secondly, “seed filling” algorithm is applied to connect broke lines to keep defect edge smoothing.

  3. Research and improvement of face detection algorithm based on the OpenCV

    Microsoft Academic Search

    Min Zuo; Guangping Zeng; Xuyan Tu

    2010-01-01

    This article describes functions and advantages of the OpenCV library, and explains the meaning, development status, applications, and difficulties of face detection technology, analysis the idea of Adaboost classifier algorithm, and achieves the detection procedures for face using OpenCV, and proposes the improved method based on the the original algorithm.

  4. A Preliminary Investigation on Computer Vision for Telemedicine Systems Using OpenCV

    Microsoft Academic Search

    Zenon Chaczko; LaReine A. Yeoh; Venkatesh Mahadevan

    2010-01-01

    OpenCV is typically, an open source vision library suitable for computer vision programs. In this paper, we present some of our preliminary investigation experiences of developing Computer Vision programs using OpenCV for robotic telemedicine cluster system, within the practice based ICTD subject within the undergraduate Software Engineering Program at the Faculty of Engineering, University of Technology Sydney (UTS). Firstly, it

  5. Chondrule Magnetizations in the Allende CV Chondrite and Implications for the Dynamo of the CV Parent Body

    NASA Astrophysics Data System (ADS)

    Weiss, B. P.; Fu, R. R.

    2012-12-01

    Many early-accreting planetesimals larger than several tens of km in diameter underwent extensive interior melting and differentiation. Advection in the molten metallic cores of these planetesimals may have generated magnetic dynamos. Remanent magnetization preserved in meteorites can reveal the past presence of core dynamo fields and therefore a metallic core on their parent bodies. Furthermore, the meteoritic magnetic record can constrain the duration of the dynamo, providing insight into the thermal evolution of the parent planetesimal. Carporzen et al. (2011) argued that bulk samples of the Allende CV carbonaceous chondrite carry a unidirectional partial thermoremanent magnetization (pTRM) blocked up to ~290C. They interpreted this magnetization as recording a magnetic core dynamo on the CV parent body. However, the previous study provided no constraints on the duration of the dynamo and did not characterize the magnetic recording in each component of the Allende meteorite. We conducted paleomagnetic experiments on 23 mutually oriented individual Allende chondrules and matrix samples. We also studied mutually oriented subsamples of 9 of these chondrules. We found that Allende chondrules can be divided into two distinct classes based on their natural remanent magnetization (NRM). Class A chondrules carry a strong low temperature overprint parallel to that of bulk Allende and matrix material that also unblocks at ~290C. Class B chondrules do not carry this low temperature overprint and exhibit randomly oriented NRMs. Electron microprobe analysis and thermal demagnetization of saturation remanence showed that magnetic phases in both Class A and Class B chondrules are likely products of parent body metasomatism. We infer that the random magnetization of Class B chondrules as well as the magnetization blocked above 290C in Class A chondrules and matrix material is a chemical remanent magnetization that resulted in randomly oriented remanence at the sub-millimeter scale. Allende chondrules therefore do not carry pre-accretional magnetization. The presence of a unidirectional pTRM overprint in Class A chondrules and matrix material but not in Class B chondrules requires a process that removed the pTRM overprint in Class B chondrules after its acquisition. We infer that, while all Allende components experienced metasomatic recrystallization of their magnetic phases, Class A chondrules completed this process before the pTRM event while Class B chondrules underwent metasomatism after the pTRM event. Furthermore, the random magnetization directions of Class B chondrules may suggest that a stable dynamo field no longer existed during the period of aqueous alteration, thereby constraining the lifetime of the CV parent body dynamo to less than several tens of My.

  6. Solution NMR Structure of Hypothetical Protein CV_2116 Encoded by a Viral Prophage Element in Chromobacterium violaceum

    SciTech Connect

    Yang, Yunhuang; Ramelot, Theresa A.; Cort, John R.; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl H.; Kennedy, Michael A.

    2012-06-14

    CV{_}2116 from Chromobacterium violaceum is a small hypothetical protein of 82 amino acids. A PSI-BLAST search using the CV{_}2116 protein sequence as a query identified only two hits, both with amino acid sequence identities of less than 40%. After the CV{_}2116 gene was cloned into the p15TvLic expression plasmid and transformed into E. coli, the desired CV{_}2116 protein was expressed and purified. A high quality solution structure of CV{_}2116 was determined by NMR spectroscopy. The tertiary structure of CV{_}2116 adopts a novel alpha + beta fold containing two anti-parallel beta sheets and one alpha helix in the C-terminal end. CV{_}2116 does not belong to any known protein sequence families and no similar structures exist in the protein data bank. To date, no function of CV{_}2116 can be derived from either sequence or structural similarity searches.

  7. Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual Machine

    E-print Network

    California at Santa Barbara, University of

    Relative Heap Efficiency of the OpenCV Framework in the Dalvik Virtual Machine and the Java Virtual jblynch@ucsd.edu Abstract The OpenCV framework is an open source project released under the BSD license will focus on the OpenCV framework, an open source project that furnishes developers with a series

  8. Remote sensing of the link between arable field and elephant ( Loxodonta africana) distribution change along a tsetse eradication gradient in the Zambezi valley, Zimbabwe

    NASA Astrophysics Data System (ADS)

    Murwira, Amon; Skidmore, Andrew K.; Huizing, H. J. G.; Prins, H. H. T.

    We investigated whether the proportion of remotely sensed arable fields increased along a tsetse eradication gradient in the Sebungwe region. We also investigated whether and to what extent this increase in arable fields affected the distribution of the African elephant ( Loxodonta africana) between the 1980s and 1990s. Results showed a relatively higher increase in the proportion of arable fields in the zone cleared of tsetse by 1986 compared to the zone that was still tsetse infested by the same date. Results also showed contrasting patterns in the relationship between the proportion of the habitat under arable fields and elephant distribution between the two periods. Specifically, in the 1980s, when arable field cover was between 0% and 11%, there was a weak ( p > 0.05) positive relationship between elephant presence and the proportion of the habitat under arable fields. In contrast, a significant ( p < 0.05) negative relationship emerged in the 1990s, when arable field cover ranged between 0% and 88%. Furthermore, the results demonstrated that the change in the probability of elephant presence between the early 1980s and the early 1990s was significantly ( p < 0.05) related to the change in the proportion arable fields. In conclusion, this study demonstrated that the expansion of arable fields in the Sebungwe was greater in areas where tsetse had been eradicated compared with areas that were still tsetse infested. Overall, the results suggest that using remotely sensed data, we can conclude that tsetse eradication led to the redistribution of elephants in response to arable field expansion.

  9. Candesartan (CV11974) dissociates slowly from the angiotensin AT 1 receptor

    Microsoft Academic Search

    Mami Ojima; Yoshiyuki Inada; Yumiko Shibouta; Takeo Wada; Tsukasa Sanada; Keiji Kubo; Kohei Nishikawa

    1997-01-01

    The mechanisms of the insurmountable antagonism of 2-ethoxy-1-[[2?-(1 H-tetrazol-5-yl)biphenyl-4-yl]methyl]-1 H-benzimidazole-7-carboxylic acid, candesartan (CV-11974), an angiotensin AT1 receptor antagonist, on angiotensin II-induced rabbit aortic contraction were examined in contraction and binding studies. Preincubation of the rabbit aorta with CV-11974 (0.1 nM) for 30 min reduced the maximal contractile response to angiotensin II by approximately 50%. This insurmountable antagonism of CV-11974 was

  10. Morphological Analyses of Spring Wheat (CIMMYT cv. PCYT-10) Somaclones

    NASA Technical Reports Server (NTRS)

    Campbell, W. F.; Carman, J. G.; Hashim, Z. N.

    1990-01-01

    The objectives of this study were to induce callus from single immature wheat embryos, produce multiple seedlings from the induced callus, and analyse the somaclonal regenerants for potential grain production in a space garden. Immature wheat, Triticum aestivum L. (cv. PCYT-10), embryos were excised 10 to 12 days post-anthesis and cultured on modified Murashige and Skoog's inorganic salts. Embryos cultured on medium containing kinetin (6-furfurylaminopurine) at 0.5mg/l plus 2 or 3mg/l dicamba (1-methoxy-3,6- dichlorobenzoic acid) or 0.2mg/l 2,4-dichlorophenoxyacetic acid produced calli from which 24, 35 and 39% of the explant tissue exhibited regenerants, respectively. The size of flag leaves, plant heights, tillers per plant, spike lengths, awn lengths, and seeds per spike were significantly different in regenerants of two-selfed recurrent generations (SC(sub 1), SC(sub 2)) than in parental controls. However, there were no significant differences in spikelets per spike between the SC(sub 2) and parental controls. Desirable characteristics that were obtained included longer spikes, more seeds per spike, supernumerary spikelets, and larger flag leaves, variants that should be useful in wheat improvement programs.

  11. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-09-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  12. Infrared small target detection technology based on OpenCV

    NASA Astrophysics Data System (ADS)

    Liu, Lei; Huang, Zhijian

    2013-05-01

    Accurate and fast detection of infrared (IR) dim target has very important meaning for infrared precise guidance, early warning, video surveillance, etc. In this paper, some basic principles and the implementing flow charts of a series of algorithms for target detection are described. These algorithms are traditional two-frame difference method, improved three-frame difference method, background estimate and frame difference fusion method, and building background with neighborhood mean method. On the foundation of above works, an infrared target detection software platform which is developed by OpenCV and MFC is introduced. Three kinds of tracking algorithms are integrated in this software. In order to explain the software clearly, the framework and the function are described in this paper. At last, the experiments are performed for some real-life IR images. The whole algorithm implementing processes and results are analyzed, and those algorithms for detection targets are evaluated from the two aspects of subjective and objective. The results prove that the proposed method has satisfying detection effectiveness and robustness. Meanwhile, it has high detection efficiency and can be used for real-time detection.

  13. [Characteristics of Papaver somniferum L. cv. ikkanshu cultivated in Izu].

    PubMed

    Iida, O; Sekine, T; Inoue, O; Yoshimatsu, K; Shimomura, K

    2000-01-01

    The seeds of Papaver somniferum L. cv. Ikkanshu were sown in November (Autumn sowing: AS) and March (Spring sowing: SS) in a field at Izu Experimental Station for Medicinal Plants of National Institute of Health Sciencs, and both AS and SS plants were cultivated to investigate their growth, opium yield and alkaloid content in the opium. Growing periods from the sowing to the opium harvest were approximately six months for AS plants and three months for SS plants. Sizes of plants and capsules in AS were bigger than those in SS, reflecting their growth period. Opium yields per an are in AS and SS were 212.09 g and 142.03 g, respectively. The opium was able to be collected four times in the AS plants though the SS plants ceased to exude opium after the second incision. Therefore higher yield of opium in AS plants seems to be attributed to an amount of opium in the third and fourth incision. Average morphine content in the total opium was 15.61% in AS plants and 15.04% in SS plants, and the estimated amounts of morphine per an are in AS and SS plants were 33.16 g and 21.38 g, respectively. PMID:11534131

  14. Limited geographic distribution of the novel cyclovirus CyCV-VN.

    PubMed

    Le, Van Tan; de Jong, Menno D; Nguyen, Van Kinh; Nguyen, Vu Trung; Taylor, Walter; Wertheim, Heiman F L; van der Ende, Arie; van der Hoek, Lia; Canuti, Marta; Crusat, Martin; Sona, Soeng; Nguyen, Hanh Uyen; Giri, Abhishek; Nguyen, Thi Thuy Chinh Bkrong; Ho, Dang Trung Nghia; Farrar, Jeremy; Bryant, Juliet E; Tran, Tinh Hien; Nguyen, Van Vinh Chau; van Doorn, H Rogier

    2014-01-01

    A novel cyclovirus, CyCV-VN, was recently identified in cerebrospinal fluid (CSF) from patients with central nervous system (CNS) infections in central and southern Vietnam. To explore the geographic distribution of this novel virus, more than 600?CSF specimens from patients with suspected CNS infections in northern Vietnam, Cambodia, Nepal and The Netherlands were screened for the presence of CyCV-VN but all were negative. Sequence comparison and phylogenetic analysis between CyCV-VN and another novel cyclovirus recently identified in CSF from Malawian patients indicated that these represent distinct cycloviral species, albeit phylogenetically closely related. The data suggest that CyCV-VN has a limited geographic distribution within southern and central Vietnam. Further research is needed to determine the global distribution and diversity of cycloviruses and importantly their possible association with human disease. PMID:24495921

  15. Impact-Induced Compaction in CV Chondrites: Exploring a Hidden Record with EBSD

    NASA Astrophysics Data System (ADS)

    Forman, L. V.; Bland, P. A.; Timms, N. E.; Benedix, G. K.

    2014-09-01

    We present impact-induced textural features found within the previously S1-classified CV, Allende. Features include high amounts of intragrain strain within the fine matrix grains, and moderate strain towards chondrule edges.

  16. Cathodoluminescence Microcharacterization of Forsterite from Kaba CV3 Chondrite: Implication for Mineralogy and Petrology of IDPs

    Microsoft Academic Search

    Sz. Bérczi; A. Gucsik; T. Okumura; K. Ninagawa; H. Nishido; Á. Kereszturi; Sz. Nagy; H. Hargitai

    2008-01-01

    Cathodoluminescence studies of forsterite from the fine fragment sample of Kaba indicate that mineral fragments from low altered and low thermally metamorphic CV3 chondrite might be potentially useful reference material for the IDPs.

  17. Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV).

    PubMed

    Shi, Min; Chen, Ya-Feng; Huang, Fang; Liu, Peng-Cheng; Zhou, Xue-Ping; Chen, Xue-Xin

    2008-06-01

    Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with IkappaB-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy. PMID:18353418

  18. Phyllosilicates in the Mokoia CV carbonaceous chondrite - Evidence for aqueous alteration in an oxidizing environment

    NASA Technical Reports Server (NTRS)

    Tomeoka, Kazushige; Buseck, Peter R.

    1990-01-01

    Most CV chondrites contain little if any phyllosilicate mineralization. A petrographic and transmission electron microscopy study of the Mokoia CV carbonaceous chondrite shows that the matrix, chondrules, aggregates, and inclusions all contain considerable amounts of phyllosilicates. The mineralogy and occurrence of phyllosilicates in Mokoia differ from those in the CI and CM chondrites. The differences suggests that aqueous alteration of the three meteorite groups probably occurred under a variety of conditions.

  19. Full split C-V method for parameter extraction in ultra thin BOX FDSOI MOS devices

    NASA Astrophysics Data System (ADS)

    Shin, Minju; Shi, Ming; Mouis, Mireille; Cros, Antoine; Josse, Emmanuel; Kim, Gyu-Tae; Ghibaudo, Gérard

    2014-09-01

    The feasibility of full split C-V method in ultra-thin body and BOX (UTBB) FDSOI devices is demonstrated, emphasizing the usefulness of gate-to-bulk capacitance. The split C-V measurements carried out on both gate-to-channel and gate-to-bulk mode are shown to be consistent with TCAD simulation. This enabled us to propose an improved parameter extraction methodology for the whole vertical FDSOI stack from gate to substrate using back biasing effect.

  20. Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa.

    PubMed

    Garigliany, Mutien-Marie; Hagen, Ralf Matthias; Frickmann, Hagen; May, Jürgen; Schwarz, Norbert Georg; Perse, Amanda; Jöst, Hanna; Börstler, Jessica; Shahhosseini, Nariman; Desmecht, Daniel; Mbunkah, Herbert Afegenwi; Daniel, Achukwi Mbunkah; Kingsley, Manchang Tanyi; Campos, Renata de Mendonca; de Paula, Vanessa Salete; Randriamampionona, Njary; Poppert, Sven; Tannich, Egbert; Rakotozandrindrainy, Raphael; Cadar, Daniel; Schmidt-Chanasit, Jonas

    2014-01-01

    Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution. PMID:25518947

  1. The comparison of CPU time consumption for image processing algorithm in Matlab and OpenCV

    Microsoft Academic Search

    Slavomir Matuska; Robert Hudec; Miroslav Benco

    2012-01-01

    In order to fill gap of growing demand for high efficient image and video processing, open source computer vision library (OpenCv) is way to deals with this task. Hence, this paper is about basic algorithm for image processing and their CPU time consumption in Matlab comparing with OpenCv. Algorithms are tested on images with resolution 3264×2448, 1920×1080, 1024×768 and 220×260.

  2. GpuCV: A GPU-Accelerated Framework for Image Processing and Computer Vision

    Microsoft Academic Search

    Yannick Allusse; Patrick Horain; Ankit Agarwal; Cindula Saipriyadarshan

    2008-01-01

    This paper presents briefly the state of the art of accelerating image processing with graphics hardware (GPU) and discusses\\u000a some of its caveats. Then it describes GpuCV, an open source multi-platform library for GPU-accelerated image processing and\\u000a Computer Vision operators and applications. It is meant for computer vision scientist not familiar with GPU technologies.\\u000a GpuCV is designed to be compatible

  3. Multi-camera calibration based on openCV and multi-view registration

    Microsoft Academic Search

    Xiao-Ming Deng; Xiong Wan; Zhi-Min Zhang; Bi-Yan Leng; Ning-Ning Lou; Shuai He

    2010-01-01

    For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the

  4. Proteins responding to drought and high-temperature stress in Populus × euramericana cv. ‘74\\/76’

    Microsoft Academic Search

    Caiyun He; Jianguo Zhang; Aiguo Duan; Shuxing Zheng; Honggang Sun; Lihua Fu

    2008-01-01

    Proteomic analysis provides a powerful method of studying plant responses to stress at the protein level. In order to study\\u000a stress-responsive molecular mechanisms for Populus × euramericana cv. ‘74\\/76’, one of the most important forest plantation tree species in subtropical and temperate regions, we analyzed the\\u000a response of 2-year-old cuttings of P. × euramericana cv. ‘74\\/76’ to drought and high

  5. Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV)

    SciTech Connect

    Shi Min; Chen Yafeng; Huang Fang; Liu Pengcheng [Institute of Insect Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029 (China); Zhou Xueping [Institute of Biotechnology, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029 (China); Chen Xuexin [Institute of Insect Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029 (China)], E-mail: xxchen@zju.edu.cn

    2008-06-05

    Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with I{kappa}B-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy.

  6. Properties of a cationic peroxidase from Citrus jambhiri cv. Adalia.

    PubMed

    Mohamed, Saleh A; El-Badry, Mohamed O; Drees, Ehab A; Fahmy, Afaf S

    2008-08-01

    The major pool of peroxidase activity is present in the peel of some Egyptian citrus species and cultivars compared to the juice and pulp. Citrus jambhiri cv. Adalia had the highest peroxidase activity among the examined species. Four anionic and one cationic peroxidase isoenzymes from C. jambhiri were detected using the purification procedure including ammonium sulfate precipitation, chromatography on diethylaminoethanol-cellulose, carboxymethyl-cellulose, and Sephacryl S-200 columns. Cationic peroxidase POII is proved to be pure, and its molecular weight was 56 kDa. A study of substrate specificity identified the physiological role of POII, which catalyzed the oxidation of some phenolic substrates in the order of o-phenylenediamine > guaiacol > o-dianisidine > pyrogallol > catechol. The kinetic parameters (K (m), V (max), and V (max)/K (m)) of POII for hydrolysis toward H2O2 and electron donor substrates were studied. The enzyme had pH and temperature optima at 5.5 and 40 degrees C, respectively. POII was stable at 10-40 degrees C and unstable above 50 degrees C. The thermal inactivation profile of POII is biphasic and characterized by a rapid decline in activity on exposure to heat. The most of POII activity (70-80%) was lost at 50, 60, and 70 degrees C after 15, 10, and 5 min of incubation, respectively. Most of the examined metal ions had a very slight effect on POII except of Li+, Zn2+, and Hg2+, which had partial inhibitory effects. In the present study, the instability of peroxidase above 50 degrees C makes the high temperature short time treatment very efficient for the inactivation of peel peroxidase contaminated in orange juice to avoid the formation of off-flavors. PMID:18633734

  7. CV Med Fellow Arrhythmia Service Rotation The goal of the Arrhythmia Service rotation is provide the CV Med Fellow with a

    E-print Network

    Ford, James

    the CV Med Fellow with a comprehensive experience in the diagnosis and treatment of cardiac arrhythmias ECG stress tests. · Describe to a physician or patient the basic elements, success rate, and risks and Cardiac Electrophysiology Laboratory Sung Chun, MD, Director, Pacemaker and ICD Service Karen Friday, MD

  8. Circulating HFMD-Associated Coxsackievirus A16 Is Genetically and Phenotypically Distinct from the Prototype CV-A16

    PubMed Central

    Li, Jingliang; Ren, Sangsang; Wei, Zhenhong; Bao, Wanguo; Hu, Xiaoming; Zhao, Ke; Zhang, Wenyan; Zhou, Yulai; Sun, Fei; Markham, Richard; Yu, Xiao-Fang

    2014-01-01

    Human enteroviruses (HEV) have been linked to hand, foot, and mouth disease (HFMD) in the Pacific and Southeast Asia for decades. Many cases of HFMD have been attributed to coxsackievirus A16 (CV-A16, CA16), based on only partial viral genome determination. Viral phenotypes are also poorly defined. Herein, we have genetically and phenotypically characterized multiple circulating CV-A16 viruses from HFMD patients and determined multiple full-length sequences of these circulating viruses. We discovered that the circulating CV-A16 viruses from HFMD patients are genetically distinct from the proto-type CV-A16 G10. We have also isolated circulating CV-A16 viruses from hospitalized HFMD patients and compared their virological differences. Interestingly, circulating CV-A16 viruses are more pathogenic in a neonatal mouse model than is CV-A16 G10. Thus, we have found circulating recombinant forms of CV-A16 (CRF CV-A16) that are related to, but different from, the prototype CV-A16 G10 that have distinct biological phenotypes. PMID:24736564

  9. Inhibition of lipid peroxidation by a novel compound (CV-2619) in brain mitochondria and mode of action of the inhibition.

    PubMed

    Suno, M; Nagaoka, A

    1984-12-28

    Lipid peroxidation in rat brain mitochondria was induced by NADH in the presence of ADP and FeCl3. CV-2619 inhibited the lipid peroxidation in a concentration-dependent manner; the concentration giving 50% inhibition (IC50) was 84 microM. In addition, the inhibitory effect of CV-2619 was strongly enhanced by adding substrates of mitochondrial respiration; when succinate, glutamate, or succinate plus glutamate was added, the IC50 of CV-2619 was changed to 1.1, 10, or 0.5 microM, respectively. Metabolites of CV-2619 also inhibited the lipid peroxidation. The inhibitory effect of CV-2619 on mitochondrial lipid peroxidation disappeared when TTFA, an inhibitor of complex II in mitochondrial respiratory chain, was added. The results indicate that in mitochondria CV-2619 is changed to its reduced form which inhibits lipid peroxidation. PMID:6517932

  10. Fusion PCR-targeted tylCV gene deletion of Streptomyces fradiae for producing desmycosin, the direct precursor of tilmicosin

    Microsoft Academic Search

    Yong Min; Heping Lv; Yinghua Zheng

    2007-01-01

    Tilmicosin is a semisynthetic macrolide antibiotic derived from tylosin. The disruption of tylCV gene from tylosin producer—Streptomyces fradiae would result in the accumulation of desmycosin, the direct precursor of tilmicosin. TylCV gene disruption cassette was constructed by fusion PCR. The tylCV replacement strain of S. fradiae was obtained through intergeneric conjugation between S. fradiae and E. coli. The antibiotic resistance

  11. Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A.

    PubMed

    Matsushima, Kazuki; Kaneda, Hirokazu; Harada, Kazuo; Matsuura, Hideyuki; Hirata, Kazumasa

    2015-05-01

    Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at -30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater. PMID:25700813

  12. Segmentation of kidney using C-V model and anatomy priors

    NASA Astrophysics Data System (ADS)

    Lu, Jinghua; Chen, Jie; Zhang, Juan; Yang, Wenjia

    2007-12-01

    This paper presents an approach for kidney segmentation on abdominal CT images as the first step of a virtual reality surgery system. Segmentation for medical images is often challenging because of the objects' complicated anatomical structures, various gray levels, and unclear edges. A coarse to fine approach has been applied in the kidney segmentation using Chan-Vese model (C-V model) and anatomy prior knowledge. In pre-processing stage, the candidate kidney regions are located. Then C-V model formulated by level set method is applied in these smaller ROI, which can reduce the calculation complexity to a certain extent. At last, after some mathematical morphology procedures, the specified kidney structures have been extracted interactively with prior knowledge. The satisfying results on abdominal CT series show that the proposed approach keeps all the advantages of C-V model and overcome its disadvantages.

  13. Discovery of a Stable Dimeric Mutant of Cyanovirin-N (CV-N) from a T7 Phage-Displayed CV-N Mutant Library

    Microsoft Academic Search

    Zhaozhong Han; Changyun Xiong; Toshiyuki Mori; Michael R. Boyd

    2002-01-01

    Mutant proteins with altered properties can be useful probes for investigating structure, ligand binding sites, mechanisms of action, and physicochemical attributes of the corresponding wild-type proteins of interest. In this report, we illuminate properties of mutants of the potent HIV-inactivating protein, cyanovirin-N (CV-N), selected by construction of a mutant library by error-prone polymerase chain reaction and affinity-based screening using T7

  14. Evaluation of antitranspirants on creeping bentgrass (Agrostis palustris Huds., cv. 'Penncros') and bermudagrass (Cynodon dactylon (L.) Pers. X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway')

    E-print Network

    Stahnke, Gwen Kristine

    1981-01-01

    . X Cynodon transvaalensis Burtt-Davy, cv. 'Tifway' (Tifway ber- mudagrass). The turfs were clipped to a 2. 5 cm mowing height during the study. Testing was done in a controlled environment chamber where conditions were chosen to give the maximum... evapotranspiration rate possible in the chamber for well-watered turf. Air temperature, point carbondioxide level, photosynthetic photon flux density, and wind speed were controlled throughout the study. Experiments lasted between 48 and 72 hours. Hater loss from...

  15. Learning OpenCV---Computer Vision with the OpenCV Library (Bradski, G.R. et al.; 2008)[On the Shelf

    Microsoft Academic Search

    Alex Zelinsky

    2009-01-01

    This is an introductory textbook for teachers, students, professionals, and hobbyists who want to learn the basics of computer vision. The book is completely based around the OpenCV library, an open source project that started in 1999 by the computer-vision community. The authors of the text are among the principal contributors to this real-time library that has developed in C\\/C++

  16. Electronic Journal of Africana Bibliography

    NSDL National Science Digital Library

    "EJAB is a refereed online journal of bibliographies on any aspect of Africa, its peoples, their homes, cities, towns, districts, states, countries, regions, including social, economic sustainable development, creative literature, the arts, and the Diaspora." The site currently has three bibliographies: Guides, Collections and Ancillary Materials to African Archival Resources in the US; Foreign Periodicals on Africa; and Medical/Health Periodicals and Books on Africa. Combined, they contain over 2100 entries. Anyone studying Africa will undoubtedly find numerous important resources.

  17. The chemical composition of suberin in apoplastic barriers affects radial hydraulic conductivity differently in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix)

    Microsoft Academic Search

    Lukas Schreiber; Rochus Franke; Klaus-Dieter Hartmann; Kosala Ranathunge; Ernst Steudle

    2005-01-01

    Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degrada- tion (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were ana- lysed quantitatively by gas chromatography and mass spectrometry. In corn, suberin was determined for isolated endodermal (ECW) and rhizo-hypodermal (RHCW) cell

  18. Martin O. Saar CV, Page 1 of 20 10/11/2010 Note: Publications are listed toward the end.

    E-print Network

    Saar, Martin O.

    Martin O. Saar CV, Page 1 of 20 10/11/2010 Note: Publications are listed toward the end. CURRICULUM VITAE Martin O. Saar E-mail: saar@umn.edu Department of Geology and Geophysics Office: +1 612 of America (GSA) 1997-date American Geophysical Union (AGU) #12;Martin O. Saar CV, Page 2 of 20 10

  19. Kenmerk: CvB 2008/879 Afspraken rond invoering UFO

    E-print Network

    Franssen, Michael

    Kenmerk: CvB 2008/879 Afspraken rond invoering UFO hulpinstrument voor secretaressefuncties Rond de invoering van een UFO hulpinstrument voor de indeling van secretaressefuncties hebben het College van vergemakkelijken. Op de beleids- en uitvoeringsafspraken van UFO heeft dit geen effect; het is een additioneel

  20. EST-SSR MARKERS FROM FRAGARIA VESCA L. CV. YELLOW WONDER

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fourteen microsatellite primer pairs were developed from a cDNA library of Fragaria vesca cv. Yellow Wonder. Transferability to 13 species of Fragaria ranged from 71% in diploid species F. gracilis Losinsk., F. iinumae Makino, F. nilgerrensis Schltdl. ex J. Gay and F. nipponica Makino to 100% in the...

  1. Outburst of CV ROTSE3 J031031.4+431115.0

    NASA Astrophysics Data System (ADS)

    Dhungana, G.; Ferrante, F. V.; Staten, R.; Kehoe, R.

    2015-02-01

    Further to ATel#1272, we report observations of an outburst of the U Geminorum-type CV ROTSE3 J031031.4+431115.0 in unfiltered CCD images taken by the 0.45 m ROTSE-IIIb telescope at McDonald Observatory, Texas.

  2. Thermal degradation of anthocyanins from purple potato (Cv. Purple Majesty) and their impact on antioxidant capacity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Degradation parameters of purified anthocyanins from purple-fleshed potato (Purple Majesty cv.) heated at high temperatures (100 - 150 °C) was determined. Purified anthocyanins, prepared by removing salts, sugars and colorless non-anthocyanin phenolics from the crude extract, were quantified using H...

  3. Alian Wang: CV (December 2013) Curriculum Vita of Prof. Alian Wang

    E-print Network

    -ASTID (Astrobiology Science and Technology Instrument Development) projects, for developing WIR and BUF sensors. 2004Alian Wang: CV (December 2013) 1 Curriculum Vita of Prof. Alian Wang Education: Ph.D. (1987 (Astrobiology Science and Technology for Exploring Planets) project, "life in Atacama". 2011 ­ 2012, PI, a NASA

  4. Simple Cp/Cv Resonance Apparatus Suitable for the Physics Teaching Laboratory.

    ERIC Educational Resources Information Center

    Smith, D. G.

    1979-01-01

    Describes a resonance apparatus for the measurement of Cp/Cv for different gases. In the apparatus a magnetically supported piston in a vertical cylindrical glass tube containing the gas is forced into oscillation by means of a standard audio signal generator. (Author/GA)

  5. Indirect measurements of Brachiaria brizantha cv. Marandu fermentable cell wall sugars for second generation biofuels production.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Results of a study conducted to evaluate the possibility of using IVDMD values of B. brizantha cv. Marandu to predict cell wall sugars that would be available in a biorefinery for ethanol production are reported. The study was conducted based on the similarity between rumen enzymes and those used i...

  6. Agrobacterium-mediated transformation of Easter lily (Lilium longiflorum cv. Nellie White)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Conditions were optimized for transient transformation of Lilium longiflorum cv. Nellie White using Agrobacterium tumefaciens. Bulb scale and basal meristem explants were inoculated with A. tumefaciens strain AGL1 containing the binary vector pCAMBIA 2301 which has the uidA gene that codes for ß-gl...

  7. The influence of nitrate nutrition on H + efflux by young rape plants ( Brassica napus cv. emerald)

    Microsoft Academic Search

    H. Moorby; P. H. Nye; R. E. White

    1985-01-01

    Changes in pH around the roots of young rape plants (Brassica napus cv. emerald) were studied using a nutrient film technique that allowed part or whole of the root system to be subjected to specific nutrient treatments. The rapidity and direction of pH change was assessed by imbedding absorbing roots in a thin film of agar containing bromocresol purple. When

  8. Antibiotic targeting of the bacterial secretory pathway Smitha Rao C.V. a

    E-print Network

    Economou, Tassos

    Review Antibiotic targeting of the bacterial secretory pathway Smitha Rao C.V. a , Evelien De xxxx Keywords: Antibiotic target Protein secretion inhibitor Signal peptidase SecA Antibiotic resistance Finding new, effective antibiotics is a challenging research area driven by novel approaches

  9. Petrological Investigations of CAIs from Efremovka and NWA 3118 CV3 Chondrites

    NASA Astrophysics Data System (ADS)

    Ivanova, M. A.; Lorenz, C. A.; Korochantseva, E. V.; MacPherson, G. J.

    2010-03-01

    Several new big CAIs were extracted from the Efremovka and NWA 3118 CV3 chondrites to analyze petrology, chemistry and isotopic compositions. Here we report preliminary results on mineralogy, petrology and bulk chemistry of two CAIs, of Type B1 and of Type A.

  10. Pre-Accretionary Distribution of Ca and Al Between Matrix and Chondrules in CV Chondrites

    NASA Astrophysics Data System (ADS)

    Hezel, D. C.; Palme, H.

    2007-03-01

    Ca/Al-ratios in Y-86751 (CV) chondrules are super- and in matrix sub-chondritic. The opposite is true for Allende and Efremovka. Incorporation of spinel in Allende and Efremovka chondrule precursors in a nebular setting can explain this observation.

  11. High productivity multiple DUT CV test for MEMS microphone wafer with automatic correction

    Microsoft Academic Search

    S. Inuzuka

    2010-01-01

    Productivity in MEMS wafer process is getting more and more important as mass production on 200 mm wafer is increasing. Multiple DUT parallel CV test is a high productive way for MEMS Microphone wafer test process, however, in case of the one of two electrodes is connected to the wafer substrate with some contact resistance, interference among DUTs has an

  12. Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The distribution of a potential biological agent, the newly discovered leafhopper-infecting virus, HoCV-1 was examined across the USA. Few biological agents exist for use against leafhopper pests. We examined and compared leafhopper salivary gland and midgut tissues using transmission electron micro...

  13. CV D. Antoine, updated August 2013 David ANTOINE, born 21 January 1965, Saint-Brieuc, France

    E-print Network

    Antoine, David

    CV D. Antoine, updated August 2013 1 David ANTOINE, born 21 January 1965, Saint-Brieuc, France, 06230 Villefranche sur mer, France Tel +33 4 93 76 37 23 Fax +33 4 93 76 37 39 antoine@obs-vlfr.fr http vitae Education 2005 D. Sc. in oceanography Université Pierre et Marie Curie / Paris 6 (UPMC), France

  14. Comportement du lupin blanc, Lupinus albus L, cv Lublanc, en sols calcaires. Seuils de tolrance

    E-print Network

    Paris-Sud XI, Université de

    Agronomie Comportement du lupin blanc, Lupinus albus L, cv Lublanc, en sols calcaires. Seuils de essai de comportement du lupin blanc, cultivar Lublanc. Ce comportement a également été observé, dans un. Les indices de pouvoir chorosant se sont révélés de moins bons estimateurs du comportement du lupin

  15. THE VALUE OF LUPINUS ALBUS L. CV. AU HOMER AS A WINTER COVER CROP FOR COTTON

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Successful cotton (Gossypium hirsutum L.) management in the southeastern USA with conservation tillage requires the utilization of winter cover crops to increase organic matter in the top 5 cm of the soil. The objective of our research was to test the newly-developed bitter white lupin cv. `AU Home...

  16. Optimized growth and plant regeneration for callus of Lilium longiflorum cv. Nellie White

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The rates of growth and regeneration were compared for compact callus, friable callus, and suspension cells of Lilium longiflorum cv. Nellie White to determine the optimal culture conditions. The fresh weight was higher for compact callus induced from bulb scales cultured on Murashige and Skoog’s m...

  17. Tolerance of mycorrhized banana ( Musa sp. cv. Pacovan) plantlets to saline stress

    Microsoft Academic Search

    Adriana Mayumi Yano-Melo; Orivaldo José Saggin; Leonor Costa Maia

    2003-01-01

    Arbuscular mycorrhizal fungi (AMF) are known to increase plant tolerance to abiotic stress, in particular soil salinity. Isolates of Acaulospora scrobiculata Trappe, Glomus clarum Nicolson & Schenck and Glomus etunicatum Becker & Gerdemann were investigated under glasshouse conditions. Inoculated banana plants (Musa sp. cv. Pacovan) showed higher nutrient contents and growth rates than the controls. G. clarum was particularly efficient

  18. Teaching image processing and pattern recognition with the Intel OpenCV library

    Microsoft Academic Search

    Adam Kozlowski; Aleksandra Królak

    2009-01-01

    In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image

  19. Registered Localization Method-based on OpenCV for Augmented Reality

    Microsoft Academic Search

    Jun Dai; Li-fen Zhang

    2011-01-01

    Augmented Reality is a kind of technique combining a virtual world with a real environment. This paper tells that OpenCV is applied to the realization of Registration Technique which is key to Augmented Reality. It studies the implementation of the camera calibration, the extraction of characteristic target and its recognition. This paper improved the efficiency of the exploitation and laid

  20. Recognition assisted dynamic surveillance system based on OSGi and OpenCV

    Microsoft Academic Search

    Jenq-Shiou Leu; Wei-Hsiang Lin; Min-Chieh Yu; Hung-Jie Tzeng

    2011-01-01

    Thanks to the Internet development, a surveillance user can monitor the distant events via a browser on a computer. These events can be even viewed on a mobile phone with the assistance from modern mobile networks. Besides, two well-known platforms - OSGi and OpenCV are widely used for application construction. OSGi is constructed to provide a service platform with high

  1. OpenCV compatible real time processor for background foreground identification

    Microsoft Academic Search

    M. Genovese; E. Napoli; N. Petra

    2010-01-01

    The background identification methods are used in many fields like video surveillance and traffic monitoring. In this paper we propose a hardware implementation of the Gaussian Mixture Model algorithm able to perform background identification on HD images. The proposed circuit is based on the OpenCV implementation, particularly suited to improve the initial background learning phase. Bit-width has been optimized in

  2. Automatic tracking of red blood cells in micro channels using OpenCV

    NASA Astrophysics Data System (ADS)

    Rodrigues, Vânia; Rodrigues, Pedro J.; Pereira, Ana I.; Lima, Rui

    2013-10-01

    The present study aims to developan automatic method able to track red blood cells (RBCs) trajectories flowing through a microchannel using the Open Source Computer Vision (OpenCV). The developed method is based on optical flux calculation assisted by the maximization of the template-matching product. The experimental results show a good functional performance of this method.

  3. Identification of HoCV-1 virus in Texas Glassy-Winged Sharpshooter Populations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A new strain of leafhopper virus was discovered in leafhoppers which are vectors of Pierce’s disease of grapes. The original leafhopper infecting virus, HoCV-1, was isolated from the glassy-winged sharpshooter, Homalodisca vitripennis, from samples collected in California and Florida. Using the orig...

  4. Pro-inflammatory effect in mice of CvL, a lectin from the marine sponge Cliona varians.

    PubMed

    Queiroz, Alexandre F S; Moura, Raniere M; Ribeiro, Jannison K C; Lyra, Ibson L; Cunha, Dayse C S; Santos, Elizeu A; de-Sales, Maurício P

    2008-03-01

    CvL, a lectin from the marine sponge Cliona varians agglutinated type A papainized erythrocytes and was strongly inhibited by d-galactose and sucrose. Models of leukocyte migration in vivo were used to study the inflammatory activity of CvL through of mouse paw oedema and peritonitis. Effect of CvL on peritoneal macrophage activation was analysed. Effects of corticoids and NSAIDS drugs were also evaluated on peritonitis stimulated by CvL. Results showed that mouse hind-paw oedema induced by subplantar injections of CvL was dose dependent until 50 microg/cavity. This CvL dose when administered into mouse peritoneal cavities induced maxima cell migration (9283 cells/microL) at 24 h after injection. This effect was preferentially inhibited by incubation of CvL with the carbohydrates d-galactose followed by sucrose. Pre-treatment of mice with 3% thioglycolate increases the peritoneal macrophage population 2.3 times, and enhanced the neutrophil migration after 24 h CvL injection (75.8%, p<0.001) and no significant effect was observed in the presence of fMLP. Finally, pre-treatment of mice with dexamethasone (cytokine antagonist) decreased (65.6%, p<0.001), diclofenac (non-selective NSAID) decreased (34.5%, p<0.001) and Celecoxib (selective NSAID) had no effect on leukocyte migration after submission at peritonitis stimulated by CvL, respectively. Summarizing, data suggest that CvL shows pro-inflammatory activity, inducing neutrophil migration probably by pathway on resident macrophage activation and on chemotaxis mediated by cytokines. PMID:17933589

  5. Modal mineralogy of CV3 chondrites by X-ray diffraction (PSD-XRD)

    NASA Astrophysics Data System (ADS)

    Howard, K. T.; Benedix, G. K.; Bland, P. A.; Cressey, G.

    2010-09-01

    Using position sensitive detector X-ray diffraction (PSD-XRD) we determine a complete modal mineralogy for all phases present in abundances greater than 1 wt% in Vigarano, Efremovka, Mokoia, Grosnaja, Kaba and Allende. Reduced CV3 samples are comprised of (vol%): olivine (83-85%); enstatite (6.5-8.1%); anorthite (1.1-1.2%); magnetite (1.4-1.8%); sulphide (2.4-5.1%); Fe, Ni metal (2-2.2%). The oxidized samples are comprised of: olivine (76.3-83.9%); enstatite (4.8-7.8%); anorthite (1.1-1.7%); magnetite (0.3-6.1%); sulphide (2.9-8.1%); Fe, Ni metal (0.2-1.1%); Fe-oxide (0-2.7%) and phyllosilicate (1.9-4.2%). When our modal data is used to calculate a bulk chemistry that is compared to literature data a near 1:1 correlation is observed. PSD-XRD data indicates that olivine compositions may span almost the entire Fe-Mg solid solution series in all CV samples and that these contain a component (4-13%) of fine-grained olivine that is more Fe-rich (>Fa 60) than is typically reported. Modal mineralogy shows that there are mineralogic differences between CV3 samples classified as oxidized and reduced but that these sub-classes are most clearly distinguished by the relative abundance of metal and Ni content of sulphide, rather than abundance of magnetite. The most significant difference in modal mineralogy observed is the relative absence of phyllosilicate in reduced CV that essentially escaped aqueous alteration. Fayalite, ferrous olivine and magnetite are typically considered secondary alteration products. The abundances of these minerals overlap in oxidized and reduced samples and correlate positively supporting common conditions of formation in a relatively oxidizing environment. The abundances of fayalite, ferrous olivine and magnetite show no relationship to petrographic type and if these abundances were used as a proxy for alteration, Allende would be the least altered CV - contrary to all previous data. The implication is that thermal metamorphism on the parent body was de-coupled from formation of Fe-rich secondary minerals. Low temperature fluid-assisted metamorphism can also not easily explain the origin of fayalite, ferrous olivine and magnetite, since the reduced CVs appear to be largely unaffected by this process. Parent body models require an anhydrous low-temperature mechanism of secondary alteration. The alternative is that these phases formed prior to accretion of the final CV parent body.

  6. Formation of biphenyl and dibenzofuran phytoalexins in the transition zones of fire blight-infected stems of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference'.

    PubMed

    Chizzali, Cornelia; Khalil, Mohammed N A; Beuerle, Till; Schuehly, Wolfgang; Richter, Klaus; Flachowsky, Henryk; Peil, Andreas; Hanke, Magda-Viola; Liu, Benye; Beerhues, Ludger

    2012-05-01

    In the rosaceous subtribe Pyrinae (formerly subfamily Maloideae), pathogen attack leads to formation of biphenyls and dibenzofurans. Accumulation of these phytoalexins was studied in greenhouse-grown grafted shoots of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference' after inoculation with the fire blight bacterium, Erwinia amylovora. No phytoalexins were found in leaves. However, both classes of defence compounds were detected in the transition zone of stems. The flanking stem segments above and below this zone, which were necrotic and healthy, respectively, were devoid of detectable phytoalexins. The transition zone of apple stems contained the biphenyls 3-hydroxy-5-methoxyaucuparin, aucuparin, noraucuparin and 2'-hydroxyaucuparin and the dibenzofurans eriobofuran and noreriobofuran. In pear, aucuparin, 2'-hydroxyaucuparin, noreriobofuran and in addition 3,4,5-trimethoxybiphenyl were detected. The total phytoalexin content in the transition zone of pear was 25 times lower than that in apple. Leaves and stems of mock-inoculated apple and pear shoots lacked phytoalexins. A number of biphenyls and dibenzofurans were tested for their in vitro antibacterial activity against some Erwinia amylovora strains. The most efficient compound was 3,5-dihydroxybiphenyl (MIC=115 ?g/ml), the immediate product of biphenyl synthase which initiates phytoalexin biosynthesis. PMID:22377689

  7. Characterization of polyphenols and evaluation of antioxidant capacity in grape pomace of the cv. Malbec.

    PubMed

    Antoniolli, Andrea; Fontana, Ariel R; Piccoli, Patricia; Bottini, Rubén

    2015-07-01

    Low molecular weight polyphenols (LMW-PPs) and anthocyanins, along with the antioxidant capacity, were assessed in grape pomace extract (GPE) of red grape (Vitis vinifera L.) cv. Malbec. Twenty-six phenolics (13 LMW-PPs and 13 anthocyanins) were characterized and quantified by HPLC-MWD and UPLC-ESI-MS. The maximum concentrations of LMW-PPs corresponded to the flavanols (+)-catechin and (-)-epicatechin, whereas malvidin-3-glucoside was the most abundant anthocyanin. Piceatannol, a stilbene analogue to resveratrol with higher antioxidant activity, was firstly identified and quantified in GPE of the cv. Malbec. The antioxidant activity for Malbec GPE determined by oxygen radical absorbance capacity (ORAC) assay was 2756?molTEg(-1) GPE. Therefore, the data reported sustain the use of winemaking by-products as a cheap source of phenolic compounds suitable for biotechnological applications, as a strategy for sustainable oenology. PMID:25704698

  8. Structural characterization of potato protease inhibitor I (Cv. Bintje) after expression in Pichia pastoris.

    PubMed

    van den Broek, Lambertus A M; Pouvreau, Laurice; Lommerse, Gijs; Schipper, Bert; Van Koningsveld, Gerrit A; Gruppen, Harry

    2004-07-28

    In the present study the structural properties of potato protease inhibitor 1 (PI-1) were studied as a function of temperature to elucidate its precipitation mechanism upon heating. A cDNA coding for PI-1 from cv. Bintje was cloned and expressed in Pichia pastoris. Using the recombinant PI-1 it was suggested that PI-1 behaves as a hexameric protein rather than as a pentamer, as previously proposed in the literature. The recombinant protein seems either to have a predominantly unordered structure or to belong to the beta-II proteins. Differential scanning calorimetry analysis of PI-1 revealed that its thermal unfolding occurs via one endothermic transition in which the hexameric PI-1 probably unfolds, having a dimer instead of a monomer as cooperative unit. The transition temperature for the recombinant PI-1 was 88 degrees C. Similar results were obtained for a partially purified pool of native PI-1 from cv. Bintje. PMID:15264937

  9. Graphical method for determining the coefficient of consolidation cv from a flow-pump permeability test

    USGS Publications Warehouse

    Morin, Roger H.; Olsen, Harold W.; Nelson, Karl R.; Gill, James D.

    1989-01-01

    A graphical method has been developed for determining the coefficient of consolidation from the transient phases of a flow-pump permeability test. The flow pump can be used to infuse fluid into or withdraw fluid from a laboratory sediment specimen at a constant volumetric rate in order to obtain data that can be used to calculate permeability using Darcy's law. Representative type-curve solutions to the associated forced-flow and pressure-decay models are derived. These curves provide the basis for graphically evaluating the permeability k, the coefficient of consolidation cv, and the coefficient of volume change mv. The curve-matching technique is easy and rapid. Values of k, cv and mv for a laterally confined kaolinite specimen were determined by this graphical method and appear to be in reasonably good agreement with numerically derived estimates (within 20%). Discrepancies between the two sets of results seem to be largely a function of data quality.

  10. Efficient and Stable Transformation of Lactuca sativa L. cv. Cisco (lettuce) Plastids

    Microsoft Academic Search

    Hirosuke Kanamoto; Atsushi Yamashita; Hiroshi Asao; Satoru Okumura; Hisabumi Takase; Masahira Hattori; Akiho Yokota; Ken-Ichi Tomizawa

    2006-01-01

    Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However,\\u000a broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation\\u000a systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene

  11. Extremely Na and Cl-rich chondrule from the CV3 carbonaceous chondrite Allende

    Microsoft Academic Search

    G. J. Wasserburg; I. D. Hutcheon; J. Aléon; E. C. Ramon; A. N. Krot; K. Nagashima; A. J. Brearley

    2011-01-01

    We report on a study of Al3509, a large Na- and Cl-rich, radially-zoned object from the oxidized CV carbonaceous chondrite Allende. Al3509 consists of fine-grained ferroan olivine, ferroan Al-diopside, nepheline, sodalite, and andradite, and is crosscut by numerous veins of nepheline, sodalite, and ferroan Al-diopside. Some poorly-characterized phases of fine-grained material are also present; these phases contain no significant H2O.

  12. A stable reddish purple anthocyanin in the leaf of Gynura aurantiaca cv. ‘Purple Passion’

    Microsoft Academic Search

    Kunijiro Yoshitama; Masahiko Kaneshige; Nariyuki Ishikura; Fukumi Araki; Shoji Yahara; Kenzo Abe

    1994-01-01

    The anthocyanin (GAA) in the epidermis and hair of the leaf ofGynura aurantiaca cv. ‘Purple Passion’ was isolated and identified as cyanidin tetra-glucoside acylated by three molecules of caffeic acid\\u000a and one molecule of malonic acid. GAA was also isolated from the lower epidermis of the leaf ofG. bicolor DC. GAA showed a very stable reddish purple color from weakly

  13. Plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis

    Microsoft Academic Search

    Wang Xiao; Xue-Lin Huang; Xia Huang; Ya-Ping Chen; Xue-Mei Dai; Jie-Tang Zhao

    2007-01-01

    A protocol for plant regeneration from protoplasts of Musa acuminata cv. Mas (AA) via somatic embryogenesis was developed. Viable protoplasts were isolated from embryogenic cell suspensions\\u000a at a yield of 1.2 × 107 protoplasts\\/ml packed cell volume (PCV). Liquid and feeder layer culture systems with medium-A and medium-B were used for\\u000a protoplast culture. In liquid culture system, medium-B was more efficient for

  14. Protein degradation and peptidase activity during petal senescence in Dendrobium cv. Khao Sanan

    Microsoft Academic Search

    Ladawan Lerslerwong; Saichol Ketsa; Wouter G. van Doorn

    2009-01-01

    During ethylene-induced petal senescence in Dendrobium cv. Khao Sanan flowers, the levels of water-insoluble protein in petals decreased but the levels of water-soluble proteins were not affected. Total peptidase activity in the petals increased from day 1 of ethylene treatment and showed a peak by day 4. Treatment of excised flowers with E-64, a specific inhibitor of cysteine peptidases, prior

  15. Alkaloid Production in Tissue Cultures of Papaver somniferum L. cv. Office-95

    E-print Network

    Esin Akçam Oluk

    The capacity of alkaloid synthesis was examined in embryogenic callus tissues of Turkish opium poppy, Papaver somniferum L cv. office-95. Eight months old cultures grown in hormone-free MS were examined for alkaloid content. They were found to produce codeine at a level of 1.2 × 10-2, the baine 2.5 × 10-3, noscapine 2.2 × 10-3, morphine 1.6 × 10-3 and papaverine 5.2 × 10-4 % FW.

  16. IV and CV curves for irradiated prototype BTeV silicon pixel sensors

    SciTech Connect

    Maria R. Coluccia et al.

    2002-07-16

    The authors present IV and CV curves for irradiated prototype n{sup +}/n/p{sup +} silicon pixel sensors, intended for use in the BTeV experiment at Fermilab. They tested pixel sensors from various vendors and with two pixel isolation layouts: p-stop and p-spray. Results are based on exposure with 200 MeV protons up to 6 x 10{sup 14} protons/cm{sup 2}.

  17. Heavily metamorphosed clasts from the CV chondrite breccias Mokoia and Yamato-86009

    NASA Astrophysics Data System (ADS)

    Jogo, Kaori; Nagashima, Kazuhide; Hutcheon, Ian D.; Krot, Alexander N.; Nakamura, Tomoki

    2012-12-01

    Abstract- Metamorphosed clasts in the CV carbonaceous chondrite breccias Mokoia and Yamato-86009 (Y-86009) are coarse-grained, granular, polymineralic rocks composed of Ca-bearing (up to 0.6 wt% CaO) ferroan olivine (Fa34-39), ferroan Al-diopside (Fs9-13Wo47-50, approximately 2-7 wt% Al2O3), plagioclase (An37-84Ab63-17), Cr-spinel (Cr/(Cr + Al) = 0.19-0.45, Fe/(Fe + Mg) = 0.60-0.79), nepheline, pyrrhotite, pentlandite, Ca-phosphate, and rare grains of Ni-rich taenite; low-Ca pyroxene is absent. Most clasts have triple junctions between silicate grains, indicative of prolonged thermal annealing. Based on the olivine-spinel and pyroxene thermometry, the estimated metamorphic temperature recorded by the clasts is approximately 1100 K. Few clasts experienced thermal metamorphism to a lower degree and preserved chondrule-like textures. The Mokoia and Y-86009 clasts are mineralogically unique and different from metamorphosed chondrites of known groups (H, L, LL, R, EH, EL, CO, CK) and primitive achondrites (acapulcoites, brachinites, lodranites). On a three-isotope oxygen diagram, compositions of olivine in the clasts plot along carbonaceous chondrite anhydrous mineral line and the Allende mass-fractionation line, and overlap with those of the CV chondrule olivines; the ?17O values of the clasts range from about -4.3‰ to -3.0‰. We suggest that the clasts represent fragments of the CV-like material that experienced metasomatic alteration, high-temperature metamorphism, and possibly melting in the interior of the CV parent asteroid. The lack of low-Ca pyroxene in the clasts could be due to its replacement by ferroan olivine during iron-alkali metasomatic alteration or by high-Ca ferroan pyroxene during melting under oxidizing conditions.

  18. Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO

    Microsoft Academic Search

    Andreas Albert; Vipaporn Sareedenchai; Werner Heller; Harald K. Seidlitz; Christian Zidorn

    2009-01-01

    Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent\\u000a field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity\\u000a of the tissues involved. Factors causing these variations were investigated by climate

  19. The Influence of Fungicide Sprays on Infection Of Apple cv. Bramley's Seedling By Nectria galligena

    Microsoft Academic Search

    Louise R. Cooke

    1999-01-01

    In 1990, a long-term trial was initiated by planting young apple trees, cv. Bramley's Seedling, inoculated, at single sites in the leader shoots, with Nectria galligena. The effect of spring–summer and autumn fungicide spray programmes, applied during 1991–1993, on the development of new cankers was assessed up to May 1994. Spring–summer fungicide programmes, applied as for the control of apple

  20. Establishment of callus and cell suspension cultures of raspberry (Rubus idaeus cv. Royalty)

    Microsoft Academic Search

    Wledzimierz Berejsza-Wysecki; Geza Hrazdin

    1994-01-01

    Callus cultures were initiated from leaf sections of raspberry (Rubus idaeus L.) cv. Royalty. Explants from younger leaves produced significantly more calli than those from older leaves. Anderson's salt mixture was more efficient for callus induction than the Murashige-Skoog medium. The best propagation and growth of calli was observed on Anderson's medium supplemented with 9 µM 2,4-dichlorophenoxyacetic acid, 4.9 µM

  1. Effects of cycocel on growth and seed yield of Lotus uliginosus Schk. cv. Grasslands Maku

    Microsoft Academic Search

    R. S. Tabora; J. G. Hampton

    1992-01-01

    Field experiments were conducted in two successive seasons to investigate the effects of the plant growth retardant cycocel applied at 1.25 and 2.50 kg a.i.\\/ha at different plant growth stages on the vegetative and reproductive growth, yield components, and seed yield of Lotus uliginosus Schk. cv. Grasslands Maku. Cycocel did not retard internode length or affect total dry matter, flowering

  2. In vitro culture of Safflower L. cv. Bhima: initiation, growth optimization and organogenesis

    Microsoft Academic Search

    T. D. Nikam; M. G. Shitole

    1998-01-01

    Callus induction and in vitro plantlet regeneration systems for safflower (Carthamus tinctorius L.) cv. Bhima using root,\\u000a hypocotyl, cotyledon and leaf explants were optimized by studying the influence on organogenesis of seedling age, media factors,\\u000a growth regulators and excision orientation. Supplementation of the medium with an auxin: cytokinin ratio < 1 enhanced the\\u000a growth rate of callus cultures; however, for

  3. Changes in leaf, stem, and root anatomy of Chrysanthemum cv. Lillian Hoek following paclobutrazol application

    Microsoft Academic Search

    G. E. Burrows; T. S. Boag; W. P. Stewart

    1992-01-01

    Plants ofChrysanthemum cv. Lillian Hoek were treated with a paclobutrazol (PBZ) soil drench and histologically examined after 3 months. PBZ application\\u000a resulted in thicker leaves, reduced stem diameter, and roots with an increased diameter and an unusual segmented appearance.\\u000a Increased leaf thickness was partly due to an additional layer of palisade mesophyll, although individual palisade cells were\\u000a shorter, of smaller

  4. Phylogenetic position of Mesorhizobium huakuii subsp. rengei, a Symbiont of Astragalus sinicus cv. Japan

    Microsoft Academic Search

    Sukma Nuswantara; Makoto Fujie; Takashi Yamada; Wanda Malek; Masaki Inaba; Yoshinobu Kaneko; Yoshikatsu Murooka

    1999-01-01

    The phylogenetic position of Rhizobium huakuii bv. renge, a symbiont of Astragalus sinicus cv. Japan (renge-sou), was studied. The following phylogenetic approaches were used: restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR) analysis of a full-length 16S rDNA fragment, 16S rDNA analysis of the first 300-bp sequence, bacteriophage typing, and amplification of the genomic region by random primer. All the

  5. An accurate method of 6H-SiC PIN structures parameter extraction using CV characteristics

    Microsoft Academic Search

    B. Tudor; G. Brezeanul; M. Badila; M. L. Locatelli; J. P. Chante; J. Millan; Ph. Godignon; A. Lebedev; N. S. Savkina

    1998-01-01

    Our paper reports an optimal parameter extraction method based on a new model for the high frequency capacitance-voltage characteristics of the 6H-SiC boron doped junction. The C-V model confirms the presence of two type regions (p- and n-) in the quasi-intrinsic layer induced by boron doping. The extracted values of the net doping of these zones (6-10×1012 cm-3) are in

  6. SALT spectral observations of a new, bright, southern CV: MASTER OT J142023.5-485540

    NASA Astrophysics Data System (ADS)

    Gulbis, A. A. S.; Kotze, M. M.; Kotze, E. J.; Worters, H. L.; Buckley, D. A. H.; O'Donoghue, H.; Shara, M.

    2013-07-01

    On 2013-06-24.063 UT, the Southern African Large Telescope (SALT; Buckley et al. 2006; O’Donoghue et al. 2006) observed MASTER OT J142023.5-485540 (RA=14h 20m 23.5s; Dec=-48d 55m 40s). This outbursting CV was discovered by MASTER-ICATE on 2013-06-08.048 UT and followup observations were requested in ATEL #5144.

  7. Blanching effects on chemistry, quality and structure of green beans (cv. Moncayo )

    Microsoft Academic Search

    Wenceslao Canet; María Dolores Alvarez; Pilar Luna; Cristina Fernández; María Estrella Tortosa

    2005-01-01

    Green beans (cv. Moncayo) were blanched at 65, 70, 75, 80, 85, 90 and 97 °C for 2.5, 5, 10, 20 and 40 min. Pectinesterase (PE) activity was highest in cell-wall-bound extracts of beans blanched at 70 °C\\/10 min. The lowest water-soluble pectin fraction, the highest EDTA-soluble pectin fraction and the lowest degree of esterification of the EDTA-soluble fraction were all recorded for the

  8. The calibration of a binocular intersection VMD measurement system based on OpenCV

    Microsoft Academic Search

    Yang Zhenhua; He Lingsong; Bei Lei

    2010-01-01

    In this paper we present a two-step stereo calibration method of binocular intersection VMD measurement system based on OpenCV. First, calibrate the two cameras separately, and get the camera's intrinsic parameters. Second, solve the extrinsic parameters from the stereo image pairs and the camera's intrinsic parameters. The experiment shows that this two-step calibration method for binocular intersection VMD measurement system

  9. Study on on-line measurement system of stone slabs based on OpenCV

    Microsoft Academic Search

    Zhao Min; Na Lihong

    2010-01-01

    An on-line measurement system of stone slabs based on CCD technology is constituted by using the CCD camera, image acquisition card, computer and so on. The on-line measurement system for the stone slab is realized by applying camera calibration and image processing algorithms. Under VC++6.0 condition, camera calibration is realized by calling OpenCV1.0 library function and stone slab contour is

  10. Highly optimized implementation of OpenCV for the Cell Broadband Engine

    Microsoft Academic Search

    Hiroki Sugano; Ryusuke Miyamoto

    2010-01-01

    Recently, real-time processing of image recognition is required for embedded applications such as automotive applications, robotics, entertainment, and so on. To realize real-time processing of image recognition on such systems we need optimized libraries for embedded processors. OpenCV is one of the most widely used libraries for computer vision applications and has many functions optimized for Intel processors, but no

  11. Research of Driver Eye Features Detection Algorithm Based on OpenCV

    Microsoft Academic Search

    Shifeng Hu; Zuhua Fang; Jie Tang; Hongbing Xu; Ying Sun

    2010-01-01

    This paper proposes a driver fatigue eye features detection algorithm based on OpenCV image processing and computer vision development platform. This algorithm localizes eye-area and detects its state based on rough to accurate thought, and can localize eye pupils in eye-open state accurately, which has significance to decrease traffic accidents. The experiment shows this algorithm can detect drivers' eye states

  12. Nature and origin of interstellar diamond from the Allende CV3 meteorite

    Microsoft Academic Search

    David Blake; Friedemann Freund; Ted Bunch; Kannan Krishnan; Mitch Stampfer; Sherwood Chang; Alexander G. G. M. Tielens

    1990-01-01

    Data and experimental evidence which support the contention that the C delta diamonds may result from grain-grain collisions in supernova shocks in the interstellar medium are presented. Fragments of the Allende CV3 chondrite were acid-treated. A whitish powder was obtained. For the Analytical Electron Microscopy (AEM) a small drop of ethanol suspension was transferred onto holey carbon support films on

  13. Requirements for plant regeneration from protoplasts of the shrubby ornamental honeysuckle, Lonicera nitida cv. Maigrun

    Microsoft Academic Search

    S. J. Ochatt

    1991-01-01

    Leaf protoplasts of axenic shoot cultures of Lonicera nitida cv Maigrun underwent sustained division to give multicellular colonies (microcalli) on a modified, ammonium-free MS (Murashige & Skoog) medium containing 0.5 mg l-1 NAA (1-naphthaleneacetic acid), 1.0 mg l-1 BAP (6-benzylaminopurine) and 150 mg l-1 casein enzymatic hydrolysate. Callus was produced upon transfer of cell colonies to MS medium with 2.0

  14. Cloning, expression and immunolocalization pattern of a cinnamyl alcohol dehydrogenase gene from strawberry (Fragariaxananassa cv. Chandler)

    Microsoft Academic Search

    R. Blanco-Portales; N. Medina-Escobar; J. A. Lopez-Raez; J. A. Gonzalez-Reyes; J. M. Villalba; E. Moyano; J. L. Caballero; J. Munoz-Blanco

    2002-01-01

    Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) catalyses the conversion of p-hydroxy-cinnamalde- hydes to the corresponding alcohols and is con- sidered a key enzyme in lignin biosynthesis. By a differential screening of a strawberry (Fragaria3 ananassa cv. Chandler) fruit specific subtractive cDNA library, a full-length clone corresponding to a cad gene was isolated (Fxacad1). Northern blot and quantitative real time PCR

  15. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds

    Microsoft Academic Search

    Pedro Chávez-Quintal; Tania González-Flores; Ingrid Rodríguez-Buenfil; Santiago Gallegos-Tintoré

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain\\u000a bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic\\u000a solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest

  16. Anthelmintic activities of aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana.

    PubMed

    Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

    2014-01-01

    Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana. PMID:24583851

  17. Umbilical reconstruction using a modified inverted C-V flap with conjoint flaps.

    PubMed

    Lee, Yoonho; Lee, San Ha; Woo, Kevin Volt

    2013-09-01

    The umbilicus is an important aesthetic feature of the abdomen. Because of its location, the umbilicus can be injured after abdominal surgical procedures. Various methods have been devised to reconstruct the umbilicus by using local flaps, purse-string sutures, or a cartilage graft, but there are no ideal methods. The authors have created a modified inverted C-V flap with conjoint flaps. A 10-year-old boy presented with deformed umbilicus because he had undergone surgical correction of an omphalocele. The drawback of the traditional C-V flap method is the transverse long abdominal scar because of the long length of the V flap. However, by using two conjoint flaps at the superior part of the C-V flap, the length of V flap can be more short and the umbilical wall can be reconstructed by rotation of two conjoint flaps. It is also good for making a sinusoidal pocket and it makes the umbilicus deeper and more natural-looking. After the operation, there were no complications like flap necrosis, infection, haematoma, and so on. The patient was satisfied with the results The patient had a more attractive umbilicus than the one with the other previous technique. This new method makes a natural-looking umbilicus with less of a transverse scar and an adequate sinusoidal pocket and umbilical wall. PMID:23829506

  18. RXJ1313.2-3259, a missing link in CV evolution?

    E-print Network

    B. T. Gaensicke; K. Beuermann; D. de Martino; H. -C. Thomas

    1999-10-25

    We present low-state IUE spectroscopy of the ROSAT-discovered polar RXJ1313.2-3259. The SWP spectrum displays a broad Lyman alpha absorption profile, which can be fitted with a two-temperature model of a white dwarf of Twd=15000K with a hot spot of Tspot=34000K which covers f~0.01 of the white dwarf surface. The white dwarf temperature is atypically low for the long orbital period (4.18h) of RXJ1313.2-3259. This low temperature implies either that the system is a young CV in the process of switching on mass transfer or that it is an older CV found in a prolonged state of low accretion rate, much below that predicted by standard evolution theory. In the first case, we can put a lower limit on the life time as pre-CV of 10e8yrs. In the second case, the good agreement of the white dwarf temperature with that expected from compressional heating suggests that the system has experienced the current low accretion rate for an extended period >10e4yrs. A possible explanation for the low accretion rate is that RXJ1313.2-3259 is a hibernating post nova and observational tests are suggested.

  19. Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana

    PubMed Central

    Lin, Rong-Jyh; Wu, Mei-Hsuan; Ma, Yi-Hsuan; Chung, Li-Yu; Chen, Chung-Yi; Yen, Chuan-Min

    2014-01-01

    Nelumbo nucifera Gaertn. cv. Rosa-plena (Nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in oriental medicine. From the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (?)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) and cepharadione B (11) were isolated and identification and anthelmintic activities of aporphine was evaluated against Anisakis simplex and Hymenolepis nana. This study found that the above constituents killed H. nana or reduced their spontaneous movements (oscillation/peristalsis). However, the above constituents at various concentrations demonstrated no larvicidal effect or ability to halt spontaneous parasite movement for 72 h against A. simplex, respectively. In addition, according to an assay of cestocidal activity against H. nana and nematocidal activity against A. simplex, we found that the above compounds showed greater lethal efficacy on H. nana than against A. simplex. Further investigation showed that these above constituents have effects against peroxyl radicals under cestocidal effect. Together, these findings suggest that these constituents of Nelumbo nucifera Gaertn. cv. Rosa-plena might be used as anthelmintic agents against H. nana. PMID:24583851

  20. Teaching image processing and pattern recognition with the Intel OpenCV library

    NASA Astrophysics Data System (ADS)

    Koz?owski, Adam; Królak, Aleksandra

    2009-06-01

    In this paper we present an approach to teaching image processing and pattern recognition with the use of the OpenCV library. Image processing, pattern recognition and computer vision are important branches of science and apply to tasks ranging from critical, involving medical diagnostics, to everyday tasks including art and entertainment purposes. It is therefore crucial to provide students of image processing and pattern recognition with the most up-to-date solutions available. In the Institute of Electronics at the Technical University of Lodz we facilitate the teaching process in this subject with the OpenCV library, which is an open-source set of classes, functions and procedures that can be used in programming efficient and innovative algorithms for various purposes. The topics of student projects completed with the help of the OpenCV library range from automatic correction of image quality parameters or creation of panoramic images from video to pedestrian tracking in surveillance camera video sequences or head-movement-based mouse cursor control for the motorically impaired.

  1. Modal Mineralogy of CV3 Chondrites by PSD-XRD: Mineralogic Insights into a Complex Evolutionary History

    NASA Astrophysics Data System (ADS)

    Howard, K. T.; Benedix, G. K.; Bland, P. A.; Cressey, G.

    2009-03-01

    CV3 chondrites Vigarano, Efremovka, Allende, Mokoia, Grosnaja and Kaba are amongst the most studied rocks in existence. By XRD we define the first quantitative modal mineralogy of these samples and explore implications of our data to petrogenesis.

  2. Antihypertensive effect of CV-4093.2HCl, a new calcium antagonist, in three rat models of hypertension.

    PubMed

    Kakihana, M; Suno, M; Nagaoka, A

    1988-10-01

    The hypotensive action of CV-4093.2HCl (CV-4093), a new calcium antagonist, was studied in spontaneously hypertensive, renal hypertensive, DOCA-salt hypertensive and normotensive rats. CV-4093 (3 and 10 mg/kg, p.o.) dose-dependently decreased systolic blood pressure in the three types of hypertensive rats. At the dose of 10 mg/kg, the compound decreased the blood pressure to the normotensive level between 1 and 3 hr after it was administered; the antihypertensive effect lasted for at least 8 hr. The systolic blood pressure in normotensive rats was also decreased at 3 and 10 mg/kg, p.o., but less evidently than in the hypertensive rats. When the antihypertensive effect of CV-4093 was compared with that of seven known calcium antagonists in spontaneously hypertensive rats, it was the most potent and the most long-lasting. PMID:3210447

  3. Defective secretion of mucilage is the cellular basis for agravitropism in primary roots of Zea mays cv. Ageotropic

    NASA Technical Reports Server (NTRS)

    Miller, I.; Moore, R.

    1990-01-01

    Root caps of primary, secondary, and seminal roots of Z. mays cv. Kys secrete large amounts of mucilage and are in close contact with the root all along the root apex. These roots are strongly graviresponsive. Secondary and seminal roots of Z. mays cv. Ageotropic are also strongly graviresponsive. Similarly, their caps secrete mucilage and closely appress the root all along the root apex. However, primary roots of Z. mays cv. Ageotropic are non-responsive to gravity. Their caps secrete negligible amounts of mucilage and contact the root only at the extreme apex of the root along the calyptrogen. These roots become graviresponsive when their tips are coated with mucilage or mucilage-like materials. Peripheral cells of root caps of roots of Z. mays cv. Kys contain many dictyosomes associated with vesicles that migrate to and fuse with the plasmalemma. Root-cap cells of secondary and seminal (i.e. graviresponsive) roots of Z. mays cv. Ageotropic are similar to those of primary roots of Z. mays cv. Kys. However, root-cap cells of primary (i.e. non-graviresponsive) roots of Z. mays cv. Ageotropic have distended dictyosomal cisternae filled with an electron-dense, granular material. Large vesicles full of this material populate the cells and apparently do not fuse with the plasmalemma. Taken together, these results suggest that non-graviresponsiveness of primary roots of Z. mays cv. Ageotropic results from the lack of apoplastic continuity between the root and the periphery of the root cap. This is a result of negligible secretion of mucilage by cells along the edge of the root cap which, in turn, appears to be due to the malfunctioning of dictyosomes in these cells.

  4. Using multidimensional Rasch analysis to validate the Chinese version of the Motivated Strategies for Learning Questionnaire (MSLQ-CV)

    Microsoft Academic Search

    John Chi-Kin Lee; Zhonghua Zhang; Hongbiao Yin

    2010-01-01

    This article used the multidimensional random coefficients multinomial logit model to examine the construct validity and detect\\u000a the substantial differential item functioning (DIF) of the Chinese version of motivated strategies for learning questionnaire\\u000a (MSLQ-CV). A total of 1,354 Hong Kong junior high school students were administered the MSLQ-CV. Partial credit model was\\u000a suggested to have a better goodness of fit

  5. Estimating variation in stomatal frequency at intra-individual, intra-site, and inter-taxonomic levels in populations of the Leonardoxa africana (Fabaceae) complex over environmental gradients in Cameroon

    NASA Astrophysics Data System (ADS)

    Finsinger, Walter; Dos Santos, Thibaut; McKey, Doyle

    2013-07-01

    Variation of stomatal frequency (stomatal density and stomatal index) includes genetically-based, potentially-adaptive variation, and variation due to phenotypic plasticity, the degree of which may be fundamental to the ability to maintain high water-use efficiency and thus to deal with environmental change. We analysed stomatal frequency and morphology (pore length, pore width) in leaves from several individuals from nine populations of four sub-species of the Leonardoxa africana complex. The dataset represents a hierarchical sampling wherein factors are nested within each level (leaves in individuals, individuals in sites, etc.), allowing estimation of the contribution of different levels to overall variation, using variance-component analysis. SI showed significant variation among sites ("site" is largely confounded with "sub-species"), being highest in the sub-species localized in the highest-elevation site. However, most of the observed variance was accounted for at intra-site and intra-individual levels. This variance could reflect great phenotypic plasticity, presumably in response to highly local variation in micro-environmental conditions.

  6. My CV

    E-print Network

    tong&zhiyi

    2015-01-24

    Tong Liu. Current Address: E-Mail: ... Research Interests: Arithmetic geometry, algebraic number theory, p-adic Galois ... p=2, to appear, Journal de Théroie des Nombres de Bordeaux. [10] A.O.L ... International Mathematics Research Notices.

  7. Compositional and petrographic similarities of CV and CK chondrites: A single group with variations in textures and volatile concentrations attributable to impact heating, crushing and oxidation

    NASA Astrophysics Data System (ADS)

    Wasson, John T.; Isa, Junko; Rubin, Alan E.

    2013-05-01

    Greenwood et al. (2010) gathered data on O-isotopic and elemental compositions and reevaluated literature data for CV and CK chondrites. They concluded that these two chondrite groups originated on the same parent asteroid, with CK chondrites being metamorphosed CV chondrites (which are otherwise missing types 4 through 6). To test this interpretation we have gathered new instrumental neutron-activation-analysis (INAA) data for CV and CK chondrites and reexamined their petrographic features. The new INAA data like the older data show scatter attributable to weathering effects, but we conclude that the refractory lithophile abundances are the same in CV and CK, in agreement with the Greenwood et al. interpretation. Several volatile elements are significantly lower in CK than in CV chondrites. Among the elements we determine, the greatest difference between CV and CK is found for Br, for which the CV/CK ratio is ?4; As and Sb are about 20% lower in CK than CV and smaller differences are observed for Zn, Ga and Se. It seems likely that volatiles were lost during impact-heating events that also provided the heat responsible for metamorphic recrystallization. Within statistical uncertainty, chondrules in CV and CK chondrites are the same size and have similar textural distributions. A significant petrographic difference between CK and CV chondrites cited by Kallemeyn et al. (1991) was the much higher percentage of igneous rims around CV chondrules. However, we now recognize that many chondrules in CK3.8 NWA 1559 have igneous rims and in CK4 chondrites, igneous rims are recognizable by their associated sulfide-rich rings; there are no quantifiable CV-CK differences in igneous-rim abundances. We used Ca and Al maps to show that CK chondrites have CAI abundances similar to those of CV chondrites. It thus appears that there are no resolvable pre-metamorphic petrographic differences between CV and CK chondrites. We recommend that the “CK” designation be abandoned and that the CV group be acknowledged as spanning the range of petrologic types 3-6. We suggest that CK3 chondrites be designated CV3OxK. Most CK4 chondrites are highly fragmental; collisional crushing appears to be much more common than in CV chondrites. It seems likely that CK chondrites formed from reduced CV3 materials after the latter was impacted, buried, aqueously altered and annealed.

  8. Unusual dark clasts in the Vigarano CV3 carbonaceous chondrite: Record of parent body process

    NASA Astrophysics Data System (ADS)

    Kojima, Tomoko; Tomeoka, Kazushige; Takeda, Hiroshi

    1993-12-01

    Two unusual dark clasts found in the Vigarano CV3 chondrite were examined using an optical microscope and a scanning electron microscope (SEM). Both clasts lack chondrules, Ca-Al-rich inclusions, and coarse-grained mineral fragments; they, instead, contain abundant inclusions that consist of fine grains (less than 1 micrometer) of homogeneous Fe-rich olivine, thus resembling the fine-grained variety of dark inclusions in CV3 chondrites. The external shapes of inclusions in the clasts bear a close resemblance to those of chondrules and chondrule fragments; some of the inclusions are surrounded by dark rims similar to chondrule rims. Our SEM observations reveal the following unusual characteristics: 1) the inclusions are not mere random aggregates of olivine grains but have peculiar internal textures, that is, assemblages of round or oval shaped outlines, which are suggestive of pseudomorphs after porphyritic olivine chondrules; 2) one of thick inclusion rims contains a network of vein-like strings of elongated olivine grains; 3) an Fe-Ni metal aggregate in one of the clasts has an Fe-, Ni-, S-rich halo suggesting a reaction between its precursor and the surrounding matrix; and 4) olivine in the clasts commonly shows a swirly, fibrous texture similar to that of phyllosilicate. These characteristics suggest that the dark clasts in Vigarano are not primary aggregates of dust in the solar nebula but were affected by aqueous alteration and subsequent dehydration by heating after accretion to the meteorite parent body. The fine olivine grains in these clasts were presumably produced by thermal transformation of phyllosilicate, as is the case with those in the two thermally metamorphosed Antarctic CM chondrites, Belgica-7904 and Yamato-86720. From textural and mineralogical similarities, some of the dark inclusions and clasts previously reported from CV3 chondrites and other types of meteorites may have origins common with these clasts in Vigarano.

  9. Potato virus Y CFH, a putative recombinant isolate from Capsicum chinense cv. Habanero.

    PubMed

    Comes, S; Fanigliulo, A; Pacella, R; Parrella, G; Crescenzi, A

    2006-01-01

    Ornamental plants of Chili pepper, Capsicum chinense cv. Habanero, with symptoms of leaf mosaic, necrotic rings on fruits and necrotic stems were observed in June 2003 in a private garden in the province of Naples (Italy). Preliminary serological characterisation allowed the association of these symptoms with infections by Potato virus Y (PVY). The virus was isolated on Nicotiana tabacum cv. Xanthi and characterised by mechanical inoculation on herbaceous hosts and molecular characterisation of the P1 and the coat protein (CP) genes. Symptoms produced on indicator plants were generally consistent with those described for PVY. The identity of PVY was further confirmed by reaction with PVYN, PVYC and PVYO specific monoclonal antibodies: the isolate reacted only with the PVYC specific Mab. Immuno capture reverse transcription polymerase chain reaction (IC-RT-PCR) was performed on extracts of PVY-CFH infected N. tabacum cv. Xanthi plants, using two couples of primers specifically designed out of the P1 and the CP coding regions of the so far fully sequenced PVY isolates. PCR products were then cloned into pCRII-TOPO vector using TOPO-TA cloning kit (Invitrogen) and sequenced. Sequence analysis suggests that PVY-CFH originated from a recombination event involving a virus of the PVYO type and another parental virus, maybe resembling the PVYNP isolates, given the reasonably high similarity shared by PVY-CFH and, respectively, non potato PVY isolates in the CP coding region, PVYO isolates in the P1 coding region. Evidence for the existence of such a recombination comes, apart from similarity analysis, by the different locations of CFH within phylogenetic trees constructed from P1 and CP genomic regions. PMID:17390887

  10. Carrier Density Profiling of Ultra-Shallow Junction Layers Through Corrected C-V Plotting

    SciTech Connect

    Chen, James; Dimitrov, Dimitar; Dimitrova, Tatiana [Four Dimensions, Inc., 3140 Diablo Ave, Hayward, California, 94545 (United States); Timans, Paul [Mattson Technology, Inc. Fremont, California (United States); Gelpey, Jeff; McCoy, Steve [Mattson Technology Canada, Inc., Vancouver (Canada); Lerch, Wilfried; Paul, Silke [Mattson Thermal Products GmbH, Dornstadt (Germany); Bolze, Detlef [IHP, Frankfurt (Oder) (Germany)

    2008-11-03

    The aim of this report is to present and justify a new approach for carrier density profiling in ultra-shallow junction (USJ) layer. This new approach is based on a capacitance measurement model, which takes series impedance, shunt resistance and the presence of a boron skin on the USJ layer into account. It allows us to extract the depletion layer capacitances in the USJ layer from C-V plotting more accurately and hence to obtain better carrier density profiles. Based on this new approach the carrier density profiles of different USJ layers with and without halo-style implants are obtained and discussed.

  11. New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

    Microsoft Academic Search

    Budi Winarto; F. Rachmawati

    A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex André cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and\\u000a Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1–1.0 mg\\/l), ?-naphthalene\\u000a acetic acid (0.01–0.2 mg\\/l), thidiazuron (0.5–2.0 mg\\/l), 6-benzylaminopurine (0.5–1.0 mg\\/l), and kinetin (0.5–1.0 mg\\/l)] were\\u000a tested for their ability

  12. Serine protease inhibitor cvSI-1 potential role in the eastern oyster host defense against the protozoan parasite Perkinsus marinus.

    PubMed

    La Peyre, Jerome F; Xue, Qing-Gang; Itoh, Naoki; Li, Yanli; Cooper, Richard K

    2010-01-01

    The serine protease inhibitor cvSI-1, purified from plasma of eastern oysters, inhibited the proliferation of the protozoan parasite Perkinsus marinus in vitro. In situ hybridization located cvSI-1 gene expression in basophil cells of the digestive tubules and cvSI-1 expression measured by real-time quantitative reverse transcriptase polymerase chain reaction was several hundred folds greater in digestive glands than in other organs examined or circulating hemocytes. cvSI-1 gene expression was also significantly greater in winter than in summer. Finally, cvSI-1 gene expression and plasma protease inhibitory activity in oysters selected for increased resistance to P. marinus were significantly greater than in unselected oysters. These findings support the hypothesis that cvSI-1 plays a role in eastern oyster host defense against P. marinus possibly through inhibition of parasite proliferation. PMID:19720077

  13. Heterogeneous compute in computer vision: OpenCL in OpenCV

    NASA Astrophysics Data System (ADS)

    Gasparakis, Harris

    2014-02-01

    We explore the relevance of Heterogeneous System Architecture (HSA) in Computer Vision, both as a long term vision, and as a near term emerging reality via the recently ratified OpenCL 2.0 Khronos standard. After a brief review of OpenCL 1.2 and 2.0, including HSA features such as Shared Virtual Memory (SVM) and platform atomics, we identify what genres of Computer Vision workloads stand to benefit by leveraging those features, and we suggest a new mental framework that replaces GPU compute with hybrid HSA APU compute. As a case in point, we discuss, in some detail, popular object recognition algorithms (part-based models), emphasizing the interplay and concurrent collaboration between the GPU and CPU. We conclude by describing how OpenCL has been incorporated in OpenCV, a popular open source computer vision library, emphasizing recent work on the Transparent API, to appear in OpenCV 3.0, which unifies the native CPU and OpenCL execution paths under a single API, allowing the same code to execute either on CPU or on a OpenCL enabled device, without even recompiling.

  14. Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO.

    PubMed

    Albert, Andreas; Sareedenchai, Vipaporn; Heller, Werner; Seidlitz, Harald K; Zidorn, Christian

    2009-05-01

    Plants in alpine habitats are exposed to many environmental stresses, in particular temperature and radiation extremes. Recent field experiments on Arnica montana L. cv. ARBO indicated pronounced altitudinal variation in plant phenolics. Ortho-diphenolics increased with altitude compared to other phenolic compounds, resulting in an increase in antioxidative capacity of the tissues involved. Factors causing these variations were investigated by climate chamber (CC) experiments focusing on temperature and ultraviolet (UV)-B radiation. Plants of A. montana L. cv. ARBO were grown in CCs under realistic climatic and radiation regimes. Key factors temperature and UV-B radiation were altered between different groups of plants. Subsequently, flowering heads were analyzed by HPLC for their contents of flavonoids and caffeic acid derivatives. Surprisingly, increased UV-B radiation did not trigger any change in phenolic metabolites in Arnica. In contrast, a pronounced increase in the ratio of B-ring ortho-diphenolic (quercetin) compared to B-ring monophenolic (kaempferol) flavonols resulted from a decrease in temperature by 5 degrees C in the applied climate regime. In conclusion, enhanced UV-B radiation is probably not the key factor triggering shifts in the phenolic composition in Arnica grown at higher altitudes but rather temperature, which decreases with altitude. PMID:19194724

  15. Plasmalemmal vesicle associated protein (PV1) modulates SV40 virus infectivity in CV-1 cells

    PubMed Central

    Tse, Dan; Armstrong, David A.; Oppenheim, Ariella; Kuksin, Dimitry; Norkin, Leonard; Stan, Radu V.

    2011-01-01

    Plasmalemmal vesicle associated protein (Plvap/PV1) is a structural protein required for the formation of the stomatal diaphragms of caveolae. Caveolae are plasma membrane invaginations that were implicated in SV40 virus entry in primate cells. Here we show that de novo Plvap/PV1 expression in CV-1 green monkey epithelial cells significantly reduces the ability of SV40 virus to establish productive infection, when cells are incubated with low concentrations of the virus. However, in presence of high viral titers PV1 has no effect on SV40 virus infectivity. Mechanistically, PV1 expression does not reduce the cell surface expression of known SV40 receptors such as GM1 ganglioside and MHC class I proteins. Furthermore, PV1 does not reduce the binding of virus-like particles made by SV40 VP1 protein to the CV-1 cell surface and does not impact their internalization when cells are incubated with either high or low VLP concentrations. These results suggest that PV1 protein is able to block SV40 infectivity at low but not at high viral concentration either by interfering with the infective internalization pathway at the cell surface or at a post internalization step. PMID:21827737

  16. Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds.

    PubMed

    Chávez-Quintal, Pedro; González-Flores, Tania; Rodríguez-Buenfil, Ingrid; Gallegos-Tintoré, Santiago

    2011-01-01

    Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative identification of the compounds present in the lowest and highest yield extracts. Analysis of the leaf extract with phytochemical tests showed the presence of alkaloids, flavonoids and terpenes. Antifungal effectiveness was determined by challenging the extracts (LE, SRE, SUE) from the best extraction treatment against three phytopathogenic fungi: Rhizopus stolonifer, Fusarium spp. and Colletotrichum gloeosporioides. The leaf extract exhibited the broadest action spectrum. The MIC(50) for the leaf extract was 0.625 mg ml(-1) for Fusarium spp. and >10 mg ml(-1) for C. gloeosporioides, both equal to approximately 20% mycelial growth inhibition. Ethanolic extracts from Carica papaya L. cv. Maradol leaves are a potential source of secondary metabolites with antifungal properties. PMID:22282629

  17. Antioxidant activity and chemical composition of Torreya grandis cv. Merrillii seed.

    PubMed

    Shi, Haiming; Wang, Huandi; Wang, Mengyue; Li, Xiaobo

    2009-11-01

    Torreya grandis cv. Merrillii is an endemic tree species in China, seeds of which are used as a popular snack, possessing beneficial effects on preventing angiosclerosis and coronary heart diseases. In this study, antioxidant activity and chemical constituents of T. grandis cv. Merrillii seed (TGMS) were investigated. The antioxidant activity of different fractions and the ethanol extract was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, hydroxyl radical scavenging activity and lipid peroxidation assays. The oil, CH2Cl2 and n-BuOH fractions, and ethanol extract of TGMS all showed antioxidant activities in these models, especially the DPPH one. By GC-MS analysis, twenty-seven constituents were identified from the oil fraction of TGMS. The total content of phenolic compounds in the CH2Cl2 and n-BuOH fractions and ethanol extract was also determined by the Folin-Ciocalteau method as 17.6, 21.6 and 12.9 microg/mg, respectively. In addition, analysis of the CH2Cl2 fraction yielded four phenolic compounds: 4-hydroxybenzaldehyde, 4-methoxy pyrocatechol, coniferyl aldehyde, 4-hydroxy cinnamaldehyde, and two steroids, beta-sitosterol and daucosterol. These results provide scientific support for the empirical use of TGMS as a medicinal food for cardiovascular diseases. PMID:19967992

  18. Antioxidant and anticancer aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena.

    PubMed

    Liu, Chi-Ming; Kao, Chiu-Li; Wu, Hui-Ming; Li, Wei-Jen; Huang, Cheng-Tsung; Li, Hsing-Tan; Chen, Chung-Yi

    2014-01-01

    Fifteen compounds were extracted and purified from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena. These compounds include liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) cepharadione B (11), ?-sitostenone (12), stigmasta-4,22-dien-3-one (13) and two chlorophylls: pheophytin-a (14) and aristophyll-C (15). The anti-oxidation activity of the compounds was examined by antiradical scavenging, metal chelating and ferric reducing power assays. The results have shown that these compounds have antioxidative activity. The study has also examined the antiproliferation activity of the isolated compounds against human melanoma, prostate and gastric cancer cells. The results shown that 7-hydroxydehydronuciferine (10) significantly inhibited the proliferation of melanoma, prostate and gastric cancer cells. Together, these findings suggest that leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena are a good resource for obtaining the biologically active substances with antioxidant properties. PMID:25372397

  19. Multi-camera calibration based on openCV and multi-view registration

    NASA Astrophysics Data System (ADS)

    Deng, Xiao-ming; Wan, Xiong; Zhang, Zhi-min; Leng, Bi-yan; Lou, Ning-ning; He, Shuai

    2010-10-01

    For multi-camera calibration systems, a method based on OpenCV and multi-view registration combining calibration algorithm is proposed. First of all, using a Zhang's calibration plate (8X8 chessboard diagram) and a number of cameras (with three industrial-grade CCD) to be 9 group images shooting from different angles, using OpenCV to calibrate the parameters fast in the camera. Secondly, based on the corresponding relationship between each camera view, the computation of the rotation matrix and translation matrix is formulated as a constrained optimization problem. According to the Kuhn-Tucker theorem and the properties on the derivative of the matrix-valued function, the formulae of rotation matrix and translation matrix are deduced by using singular value decomposition algorithm. Afterwards an iterative method is utilized to get the entire coordinate transformation of pair-wise views, thus the precise multi-view registration can be conveniently achieved and then can get the relative positions in them(the camera outside the parameters).Experimental results show that the method is practical in multi-camera calibration .

  20. Biochemical markers assisted screening of Fusarium wilt resistant Musa paradisiaca (L.) cv. puttabale micropropagated clones.

    PubMed

    Venkatesh; Krishna, V; Kumar, K Girish; Pradeepa, K; Kumar, S R Santosh; Kumar, R Shashi

    2013-07-01

    An efficient protocol was standardized for screening of panama wilt resistant Musa paradisiaca cv. Puttabale clones, an endemic cultivar of Karnataka, India. The synergistic effect of 6-benzyleaminopurine (2 to 6 mg/L) and thidiazuron (0.1 to 0.5 mg/L) on MS medium provoked multiple shoot induction from the excised meristem. An average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/L 6-benzyleaminopurine and 0.3 mg/L thidiazuron concentrations. Elongation of shoots observed on 5 mg/L BAP augmented medium with a mean length of 8.38 +/- 0.30 shoots per propagule. For screening of disease resistant clones, multiple shoot buds were mutated with 0.4% ethyl-methane-sulfonate and cultured on MS medium supplemented with Fusarium oxysporum f. sp. cubense (FOC) culture filtrate (5-15%). Two month old co-cultivated secondary hardened plants were used for screening of disease resistance against FOC by the determination of biochemical markers such as total phenol, phenylalanine ammonia lyase, oxidative enzymes like peroxidase, polyphenol oxidase, catalase and PR-proteins like chitinase, beta-1-3 glucanase activities. The mutated clones of M. paradisiaca cv. Puttabale cultured on FOC culture filtrate showed significant increase in the levels of biochemical markers as an indicative of acquiring disease resistant characteristics to FOC wilt. PMID:23898552

  1. Chemical and physical studies of type 3 chondrites 12: The metamorphic history of CV chondrites and their components

    NASA Technical Reports Server (NTRS)

    Guimon, R. Kyle; Symes, Steven J. K.; Sears, Derek W. G.

    1995-01-01

    The induced thermoluminescence (TL) properties of 16 CV and CV-related chondrites, four CK chondrites and Renazzo (CR2) have been measured in order to investigate their metamorphic history. The petrographic, mineralogical and bulk compositional differences among the CV chondrites indicate that the TL sensitivity of the approximately 130 C TL peak is reflecting the abundance of ordered feldspar, especially in chondrule mesostasis, which in turn reflects parent-body metamorphism. The TL properties of 18 samples of homogenized Allende powder heated at a variety of times and temperatures, and cathodoluminescence mosaics of Axtell and Coolidge, showed results consistent with this conclusion. Five refractory inclusions from Allende, and separates from those inclusions, were also examined and yielded trends reflecting variations in mineralogy indicative of high peak temperatures (either metamorphic or igneous) and fairly rapid cooling. The CK chondrites are unique among metamorphosed chondrites in showing no detectable induced TL, which is consistent with literature data that suggests very unusual feldspar in these meteorites. Using TL sensitivity and several mineral systems and allowing for the differences in the oxidized and reduced subgroups, the CV and CV-related meteorites can be divided into petrologic types analogous to those of the ordinary and CO type 3 chondrites. Axtell, Kaba, Leoville, Bali, Arch and ALHA81003 are type 3.0-3.1, while ALH84018, Efremovka, Grosnaja, Allende and Vigarano are type 3.2-3.3 and Coolidge and Loongana 001 are type 3.8. Mokoia is probably a breccia with regions ranging in petrologic type from 3.0 to 3.2. Renazzo often plots at the end of the reduced and oxidized CV chondrite trends, even when those trends diverge, suggesting that in many respects it resembles the unmetamorphosed precursors of the CV chondrites. The low-petrographic types and low-TL peak temperatures of all samples, including the CV3.8 chondrites, indicates metamorphism in the stability field of low feldspar (i.e., less than 800 C) and a metamorphic history similar to that of the CO chondrites but unlike that of the ordinary chondrites.

  2. Determination of the petrologic type of CV3 chondrites by Raman spectroscopy of included organic matter

    NASA Astrophysics Data System (ADS)

    Bonal, Lydie; Quirico, Eric; Bourot-Denise, Michèle; Montagnac, Gilles

    2006-04-01

    This paper reports the first reliable quantitative determination of the thermal metamorphism grade of a series of nine CV3 chondrites: Allende, Axtell, Bali, Mokoia, Grosnaja, Efremovka, Vigarano, Leoville, and Kaba. The maturity of the organic matter in matrix, determined by Raman spectroscopy, has been used as a powerful metamorphic tracer, independent of the mineralogical context and extent of aqueous alteration. This tracer has been used along with other metamorphic tracers such as Fe zoning in type-I chondrules of olivine phenocrysts, presolar grain abundance and noble gas abundance (bulk and P3 component). The study shows that the petrologic types determined earlier by Induced ThermoLuminescence were underestimated and suggests the following values: PT (Allende-Axtell) >3.6; PT (Bali-Mokoia-Grosnaja) ˜3.6; PT (Efremovka-Leoville-Vigarano) = 3.1-3.4; PT (Kaba) ˜3.1. The most commonly studied CV3, Allende, is also the most metamorphosed. Bali is a breccia containing clasts of different petrologic types. The attribution suggested by this study is that of clasts of the highest petrologic types, as pointed out by IOM maturity and noble gas bulk abundance. CV3 chondrites have complex asteroidal backgrounds, with various degrees of aqueous alteration and/or thermal metamorphism leading to complex mineralogical and petrologic patterns. (Fe,Mg) chemical zoning in olivine phenocrysts, on the borders of type I chondrules of porphyritic olivine- and pyroxene-rich textural types, has been found to correlate with the metamorphism grade. This suggests that chemical zoning in some chondrules, often interpreted as exchanges between chondrules and nebular gas, may well have an asteroidal origin. Furthermore, the compositional range of olivine matrix is controlled both by thermal metamorphism and aqueous alteration. This does not support evidence of a nebular origin and does not necessarily mirror the metamorphism grade through (Fe,Mg) equilibration. On the other hand, it may provide clues on the degree of aqueous alteration vs. thermal metamorphism and on the timing of both processes. In particular, Mokoia experienced significant aqueous alteration after the metamorphism peak, whereas Grosnaja, which has similar metamorphism grade, did not.

  3. YEAST dynamics during the natural fermentation process of table olives (Negrinha de Freixo cv.).

    PubMed

    Pereira, Ermelinda L; Ramalhosa, Elsa; Borges, Ana; Pereira, José A; Baptista, Paula

    2015-04-01

    Yeast population and dynamics associated to spontaneous fermentation of green table olives Negrinha de Freixo cv. were evaluated. Olives and brine samples were taken at different fermentation times, and yeast were enumerated by standard plate count and identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (rDNA). Saccharomyces cerevisiae was the most frequent, followed by Candida tropicalis, Pichia membranifaciens and Candida boidini, representing together 94.8% of the total isolates. Galactomyces reessii was also identified for the first time in table olives. The highest species diversity was found between 44 and 54 days of fermentation, both in brine and olive pulp. Furthermore, high similarity was observed between brine and olive pulp microbiotas. In conclusion, these results give valuable information to table olive industrials in order to achieve more knowledge on the fermentation process of this important Protected Designation of Origin product. PMID:25475331

  4. Partial melting of the Allende (CV3) meteorite - Implications for origins of basaltic meteorites

    NASA Astrophysics Data System (ADS)

    Jurewicz, A. J. G.; Mittlefehldt, D. W.; Jones, J. H.

    1991-05-01

    Eucrites and angrites are distinct types of basaltic meteorites whose origins are poorly known. Experiments in which samples of the Allende (CV3) carbonaceous chondrite were partially melted indicate that partial melts can resemble either eucrites or angrites, depending only on the oxygen fugacity. Melts are eucritic if this variable is below that of the iron-wuestite buffer or angritic if above it. With changing pressure, the graphite-oxygen redox reaction can produce oxygen fugacities that are above or below those of the iron-wuestite buffer. Therefore, a single, homogeneous, carbonaceous planetoid greater than 110 kilometers in radius could produce melts of drastically different composition, depending on the depth of melting.

  5. Inducing gravitropic curvature of primary roots of Zea mays cv Ageotropic

    NASA Technical Reports Server (NTRS)

    Moore, R.; Evans, M. L.; Fondren, W. M.

    1990-01-01

    Primary roots of the mutant 'Ageotropic' cultivar of Zea mays are nonresponsive to gravity. Their root caps secrete little or no mucilage and touch the root only at the extreme apex. A gap separates the cap and root at the periphery of the cap. Applying mucilage from normal roots or substances with a consistency similar to that of mucilage to tips of mutant roots causes these roots to become strongly graviresponsive. Gravicurvature stops when these substances are removed. Caps of some mutants secrete small amounts of mucilage and are graviresponsive. These results indicate that (a) the lack of graviresponsiveness in the mutant results from disrupting the transport pathway between the cap and root, (b) movement of the growth-modifying signal from the cap to the root occurs via an apoplastic pathway, and (c) mucilage is necessary for normal communication between the root cap and root in Zea mays cv Ageotropic.

  6. Cucurbitane and hexanorcucurbitane glycosides from the fruits of Cucurbita pepo cv dayangua.

    PubMed

    Wang, Da-Cheng; Pan, Hong-Yu; Deng, Xu-Ming; Xiang, Hua; Gao, Hui-Yuan; Cai, Hui; Wu, Li-Jun

    2007-01-01

    Phytochemical investigation of the fruits of Cucurbita pepo cv dayangua has led to the isolation of two cucurbitane glycosides: cucurbitacin L 2-O-beta-D-glucopyranoside (1), cucurbitacin K 2-O-beta-D-glucopyranoside (2) and two hexanorcucurbitane glycosides: 2,16-dihydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 2-O-beta-D-glucopyranoside (3) and 16-hydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranoside (4). Compounds 1, 2 and 3 were isolated from Cucurbita genus for the first time, while compound 4 is a new one. Their structures were determined on the basis of chemical and spectroscopic evidence. PMID:17885839

  7. Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering.

    PubMed

    Sahni, Sumit; Ganie, Showkat Hussain; Narula, Alka; Srivastava, Prem Shankar; Singh, Hari Bansh

    2013-07-01

    High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B. juncea cv. Geeta. Hypocotyl from 5-day-old seedlings were used as explants. Of the various combinations of auxins and cytokinins tried along with Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium, MS + IAA (0.2 mg/l) + BA (3 mg/l) proved best for shoot regeneration with 89.9 % regeneration efficiency. To induce early flowering Leafy gene from Arabidopsis thaliana was transformed using Agrobacterium mediated transformation method. After 12 weeks transgenic plants showed flowering in vitro whereas their untransformed counterpart did not flower even after 16 weeks. The maximum transformation frequency was 4 %. PMID:24431514

  8. Petrography and classification of refractory inclusions in the Allende and Mokoia CV3 chondrites

    NASA Technical Reports Server (NTRS)

    Kornacki, A. S.; Cohen, R. E.; Wood, J. A.

    1983-01-01

    Results are reported for a comprehensive petrographic survey of the refractory inclusions in the Allende and Mokoia CV3 chondrites. More than 600 refractory inclusions in 22 thin sections of the meteorites were studied by optical and scanning-electron microscopy. Olivine-rich inclusions and Ca, Al-rich inclusions (CAIs) are aggregates of various combinations of three fundamental petrographic constituents: rimmed concentric objects, Ca, Si-rich chaotic material, and mafic inclusion matrix. A new classification system for refractory inclusions is developed that is based on the size and abundance of these three fundamental constituents. The new classification system avoids several problems that are inherent in other classification systems, which use the term 'coarse-grained' too restrictively for many simple CAIs and inaccurately for most mililite-rich complex CAIs.

  9. Direct and efficient plant regeneration from leaf explants of Solanum tuberosum l. cv. Bintje.

    PubMed

    Yadav, N R; Sticklen, M B

    1995-07-01

    A two-step procedure was used for plant regeneration from in vitro grown leaf strips (2-3 mm wide) of cv. Bintje. Step I medium was designed with 2,4-dichlorophenoxycetic acid (2,4-D) at 0.0 or 9.0 ?M, in combination with 2.28 ?M kinetin (K), benzyl adenine (BA), zeatin (Z) or zeatin riboside (ZR). Step II media were 2,4-D-free media containing 5.78 ?M gibberellic acid (GA3) and growth regulators similar to those of step I media. Leaf explants cultured in medium I containing zeatin riboside or zeatin for 6 days and then subcultured in medium II containing zeatin riboside produced numerous shoots without callus formation. Zeatin riboside containing step I and II media caused shoot regeneration in a high number (97.5±2.2) of explants. Approximately, 33.7±8.4 shoots were regenerated from each leaf explant. PMID:24194313

  10. Flight-determined derivatives and dynamic characteristics of the CV-990 airplane

    NASA Technical Reports Server (NTRS)

    Gilyard, G. B.

    1972-01-01

    Flight-determined longitudinal and lateral-directional stability and control derivatives are presented for the CV-990 airplane for various combinations of Mach number, altitude, and flap setting throughout the flight envelope up to a Mach number of 0.87. Also presented are the dynamic characteristics of the aircraft calculated from the flight-obtained derivatives and the measured phugoid characteristics. The derivative characteristics were obtained from flight records of longitudinal and lateral-directional transient oscillation maneuvers by using a modified Newton-Raphson digital derivative determination technique. Generally the derivatives exhibited consistent variation with lift coefficient in the low-speed data and with Mach number and altitude in the high-speed data. Many also varied with flap deflection, notably spoiler effectiveness and directional stability.

  11. Overseas trip report, CV 990 underflight mission. [Norwegian Sea, Greenland ice sheet, and Alaska

    NASA Technical Reports Server (NTRS)

    Gloersen, P.; Crawford, J.; Hardis, L.

    1980-01-01

    The scanning microwave radiometer-7 simulator, the ocean temperature scanner, and an imaging scatterometer/altimeter operating at 14 GHz were carried onboard the NASA CV-990 over open oceans, sea ice, and continental ice sheets to gather surface truth information. Data flights were conducted over the Norwegian Sea to map the ocean polar front south and west of Bear Island and to transect several Nimbus-7 footprints in a rectangular pattern parallel to the northern shoreline of Norway. Additional flights were conducted to obtain correlative data on the cryosphere parameters and characteristics of the Greenland ice sheet, and study the frozen lakes near Barrow. The weather conditions and flight path way points for each of the nineteen flights are presented in tables and maps.

  12. Bioresorbable adhesion barrier for reducing the severity of postoperative cardiac adhesions: Focus on REPEL-CV®

    PubMed Central

    Haensig, Martin; Mohr, Friedrich Wilhelm; Rastan, Ardawan Julian

    2011-01-01

    Treatment of a number of congenital heart defects often necessitates staged surgical intervention. In addition, substantial improvements in postoperative cardiac care and more liberal use of biological valve substitutes have resulted in many adult patients surviving to become potential candidates for reoperations to repair or replace valves or to undergo additional revascularization procedures. In all these scenarios, surgeons are confronted with cardiac adhesions, leading to an increased surgical risk. Thus, bioresorbable adhesion barriers had become of increasing interest because they are easy to use, and safe and effective. This review focuses on the mechanisms by which REPEL-CV® prevents adhesive processes, as well as the development, design, and materials used, and also summarizes efficacy studies, clinical data, safety, and current role in therapy. PMID:22915926

  13. Postharvest treatments with ethylene on Vitis vinifera (cv Sangiovese) grapes affect berry metabolism and wine composition.

    PubMed

    Becatti, Elisa; Genova, Giuseppe; Ranieri, Annamaria; Tonutti, Pietro

    2014-09-15

    Grapes (Vitis vinifera, cv Sangiovese), harvested at standard commercial maturity, were treated for 36 h with ethylene (ET, 1000 ppm) or air (control, CT) before vinification. The composition of the grapes, must and wine was different in the CT and ET samples. In the ET wine, higher concentrations of specific phenol compounds, belonging to the classes of flavonols, anthocyanins, flavan-3-ols, and stilbenes, were detected. ET induced a significant change in the wine aroma profile by increasing free volatile categories such as phenols and fatty acids, and reducing the content of carbonyl compounds and, in particular, of esters. Less pronounced differences between CT and ET wines were observed in terms of glycosidically-bound volatile compounds. The activity of pectin methyl esterase and ?-glucosidase was enhanced in ET-treated berry skins, suggesting that cell wall properties and changes in the hydrolytic activity are effective in modulating the composition of CT and ET wines. PMID:24767053

  14. Effects of agricultural practices on color, carotenoids composition, and minerals contents of sweet peppers, cv. Almuden.

    PubMed

    Pérez-López, Antonio José; López-Nicolas, José Manuel; Núñez-Delicado, Estrella; Del Amor, Francisco M; Carbonell-Barrachina, Angel A

    2007-10-01

    Consumers demand organic products because they believe they are more flavorful and respectful to the environment and human health. The effects of conventional, integrated, and organic farming, grown in a controlled greenhouse, on color, minerals, and carotenoids of sweet pepper fruits ( Capsicum annuum), cv. Almuden, were studied. Experimental results proved that organic farming provided peppers with the highest (a) intensities of red and yellow colors, (b) contents of minerals, and (c) total carotenoids. Integrated fruits presented intermediate values of the quality parameters under study, and conventional fruits were those with the lowest values of minerals, carotenoids, and color intensity. As an example, the concentrations of total carotenoids were 3231, 2493, and 1829 mg kg (-1) for organic, integrated, and conventional sweet peppers, respectively. Finally, organic red peppers could be considered as those having the highest antioxidant activity of all studied peppers (agricultural farming and development stage). PMID:17822289

  15. Indicain, a dimeric serine protease from Morus indica cv. K2.

    PubMed

    Singh, Vijay Kumar; Patel, Ashok Kumar; Moir, A J; Jagannadham, Medicherla V

    2008-08-01

    A high molecular mass serine protease has been purified to homogeneity from the latex of Morus indica cv. K2 by the combination of techniques of ammonium sulfate precipitation, hydrophobic interaction chromatography, and size-exclusion chromatography. The protein is a dimer with a molecular mass of 134.5 kDa and with two monomeric subunits of 67.2 kDa and 67.3 (MALDI-TOF), held by weak bonds susceptible to disruption on exposure to heat and very low pH. Isoelectric point of the enzyme is pH 4.8. The pH and temperature optima for caseinolytic activity were 8.5 and 80 degrees C, respectively. The extinction coefficient (epsilon280(1%)) of the enzyme was estimated as 41.24 and the molecular structure consists of 52 tryptophan, 198 tyrosine and 42 cysteine residues. The enzyme activity was inhibited by phenylmethylsulfonylflouride, chymostatin and mercuric chloride indicating the enzyme to be a serine protease. The enzyme is fairly stable and similar to subtilases in its stability toward pH, strong denaturants, temperature, and organic solvents. Polyclonal antibodies specific to enzyme and immunodiffusion studies reveal that the enzyme has unique antigenic determinants. The enzyme has activity towards broad range of substrates comparable to those of subtilisin like proteases. The N-terminal residues of indicain (T-T-N-S-W-D-F-I-G-F-P) exhibited considerable similarity to those of other known plant subtilases, especially with cucumisin, a well-characterized plant subtilase. This is the first report of purification and characterization of a subtilisin like dimeric serine protease from the latex of M. indica cv. K2. Owing to these unique properties the reported enzyme would find applications in food and pharma industry. PMID:18561962

  16. Quantifying key parameters as elicitors for alternate fruit bearing in cv. 'Elstar' apple trees.

    PubMed

    Krasniqi, Anne-Lena; Damerow, Lutz; Kunz, Achim; Blanke, Michael M

    2013-11-01

    The commonly known alternate bearing, i.e. year-to-year change of large and small yields of fruit tree crops worldwide, is often induced by abiotic stress such as late frost, which will eliminate flowers or fruitlets. This study presents an alternative form, biotic biennial bearing, i.e. change of large and small yields of the same trees within the same tree row in the same year. Three methods were developed or modified for the analysis of the number of flower clusters and yield of 2086 apple (Malus domestica Borkh.) cv. 'Elstar' trees. The first method, i.e., based on intersect between yield in year x and year x+1 and flower clusters in year x, yielded 91-106 flower clusters, whereas the second method, i.e., mean yield in year x and year x+1, resulted in a range of 72-133 flower clusters, or 9.6kg/tree necessary for sustainable cultivation of apple cv. 'Elstar'. The third 'biennial bearing index' (BBI), was calculated in three ways as the ratio of differences in tree yields to cumulative tree yield, for individual trees (rather than orchard average) to demonstrate the tree-to-tree alternation. A scheme for the possible underlying regulatory mechanisms was developed, which includes potential elicitors such as light deprivation and subsequent lack of flower initiation, are discussed as a possible result of polar basipetal GA7 transport, cytokinin level in the xylem and phloem and down-regulation of the gene expression of the flowering gene. Suggested countermeasures included early chemical or mechanical thinning. PMID:24094049

  17. Economic valuation of the Seto Inland Sea by using an Internet CV survey.

    PubMed

    Tsuge, Takahiro; Washida, Toyoaki

    2003-01-01

    We estimate the economic value of the natural environment damaged in the Seto Inland Sea after the introduction of the Law on Temporary Measures for the Environmental Conservation of the Seto Inland Sea (Setouchi Law) and the value of the natural environment that survived, using a Contingent Valuation (CV) survey on an Internet web site. The CV survey contains three plans. Plan 1 is to restore 4 ha of reclaimed land. By estimating the Willingness To Pay (WTP) for plan 1, we can appraise the value of the natural environment that was damaged as a result of the original reclamation. Plan 2 is to transplant Zostera (eel-grass) into an area of 10 ha offshore. Plan 3 is to preserve the shore area, a natural habitat for rare animal species, under the National Trust Program. From the WTP for plans 2 and 3, we can estimate the value of the shore area and the areas a little farther offshore. The value of the natural environment damaged in the Seto Inland Sea as a result of reclaiming projects after the introduction of the Setouchi Law and the value of the existing natural environment of the Seto Inland Sea from the WTP for the plans were estimated to about 172 trillion yen (1.46 trillion dollars) and about 424 trillion yen (3.60 trillion dollars), respectively. The results indicate that in the 25 years since the introduction of the Setouchi Law, we have degraded every year about 6.88 trillion yen (58.5 billion dollars) worth of the natural environment by reclaiming. Some seaweed farms and natural shore areas, natural habitats to rare marine life-forms like the horseshoe crab and the fiddler crab have survived, but their value amounts to about 80% of Japan's GDP. PMID:12787625

  18. Synchronizing the Absolute and Relative Clocks: Pb-Pb and Al-Mg Systematics in CAIs from the Allende and NWA 2364 CV3 Chondrites

    NASA Astrophysics Data System (ADS)

    Bouvier, A.; Wadhwa, M.

    2009-03-01

    A Pb-Pb internal isochron of a type-B CAI from the NWA 2364 CV3 chondrite gives an absolute age of 4568.6 ± 0.2 Ma which contrasts with previous internal Pb-Pb ages of CAIs from Allende and Efremovka. Al-Mg systematics are also reported for CV3 CAIs.

  19. Toward positional cloning of everblooming gene (evb) in plants: a BAC library of Rosa chinensis cv. old blush

    E-print Network

    Hess, Gregory

    2006-10-30

    megabase-size DNA was isolated from the recurrent blooming diploid species, Rosa chinensis cv. Old Blush. The DNA was then partially digested with BamHI and separated on agarose gels by multi-phase pulsed-field gel electrophoresis. Size selected fragments...

  20. First report of downy mildew caused by Plasmopara halstedii on black-eyed susan (Rudbeckia fulgida cv. ‘Goldsturm’) in Maryland

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The North American perennial black-eyed Susan (Rudbeckia fulgida cv. ‘Goldsturm’) is an important nursery crop, prized by gardeners and landscapers for its persistent bloom and ease of cultivation. In September 2013 disease symptoms characteristic of downy mildew were observed from multiple plants a...

  1. Extraction of arbutin and its comparative content in branches, leaves, stems, and fruits of Japanese pear Pyrus pyrifolia cv. Kousui.

    PubMed

    Sasaki, Chizuru; Ichitani, Masaki; Kunimoto, Ko-Ki; Asada, Chikako; Nakamura, Yoshitoshi

    2014-01-01

    Arbutin is a tyrosinase inhibitor and is extensively used as a human skin-whitening agent. This study investigated the optimum conditions for extracting arbutin by ultrasonic homogenization from discarded branches pruned from Japanese pear (Pyrus pyrifolia cv. Kousui) trees. The arbutin content was measured in the branches and also in the leaves, stems, fruit peel, and fruit flesh. PMID:25035992

  2. Are the input parameters of white noise driven integrate and fire neurons uniquely determined by rate and CV?

    E-print Network

    Lindner, Benjamin

    by rate and CV? Rafael D. Vilela �, Benjamin Lindner Max-Planck-Institut fu¨r Physik komplexer Systeme, No IF models have been proposed, the firing statistics of which depends nontrivially on the input parameters m is associated with a given set of basic firing statistics as, for instance, the firing rate and the coefficient

  3. Are the input parameters of white noise driven integrate and fire neurons uniquely determined by rate and CV?

    PubMed

    Vilela, Rafael D; Lindner, Benjamin

    2009-03-01

    Integrate and fire (IF) neurons have found widespread applications in computational neuroscience. Particularly important are stochastic versions of these models where the driving consists of a synaptic input modeled as white Gaussian noise with mean mu and noise intensity D. Different IF models have been proposed, the firing statistics of which depends nontrivially on the input parameters mu and D. In order to compare these models among each other, one must first specify the correspondence between their parameters. This can be done by determining which set of parameters (mu,D) of each model is associated with a given set of basic firing statistics as, for instance, the firing rate and the coefficient of variation (CV) of the interspike interval (ISI). However, it is not clear a priori whether for a given firing rate and CV there is only one unique choice of input parameters for each model. Here we review the dependence of rate and CV on input parameters for the perfect, leaky, and quadratic IF neuron models and show analytically that indeed in these three models the firing rate and the CV uniquely determine the input parameters. PMID:19063904

  4. Wintercuring of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid in response to Xylella fastidiosa infections

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Clonal replicates of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid P 63-61 were inoculated with Xylella fastidiosa strain M23 and evaluated for Almond Leaf Scorch Disease and subsequent wintercuring of infections during three growing seasons. Initial inoculations established gr...

  5. Water Deficit Effect on Ratio of Seed to Berry Fresh Weight and Berry Weight Uniformity in Winegrape cv. Merlot

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Field-grown grapevines cv. Merlot (Vitis vinifera L.) were differentially irrigated in a randomized block design during two growing seasons to maintain a high or low level of vine water stress between fruit set and harvest. Detached berries from clusters harvested at maturity were individually weig...

  6. Effect of Cutting Heights on Productivity and Quality of King Napier Grass (Pennisetum purpureum cv. King Grass) under Irrigation

    Microsoft Academic Search

    Sumran Wijitphan; Pornchai Lorwilai; Chutipong Arkaseang

    2009-01-01

    2 Abstract: An experiment to study the effect of cutting heights on yield and nutritive values of King napier grass (Pennisetum purpureum cv. King grass) was conducted on sandy loam, Korat soil series (Oxic Paleustults) under irrigation during June 2006 to November 2007 at Khon Kaen Animal Nutrition Development Research Center, Thailand. Four treatments of cutting height at 0 (T1),

  7. Vis\\/NIR soluble solids prediction in intact oranges ( Citrus sinensis L.) cv. Valencia Late by reflectance

    Microsoft Academic Search

    J. A. Cayuela

    2008-01-01

    A commercially available instrument of near infrared spectroscopy, equipped with a post-dispersive reflectance configuration, was used for the development of partial least squares calibration models for soluble solids content and total acidity prediction in oranges (Citrus sinensis L.) cv. Valencia Late. Both internal validation and external validation were conducted and modified partial least squares regression was also tested. The best

  8. A hierarchical neural network model based on a C\\/V segmentation algorithm for isolated Mandarin speech recognition

    Microsoft Academic Search

    Jhing-Fa Wang; Chung-Hsien Wu; Shih-Hung Chang; Jau-Yien Lee Lee

    1991-01-01

    A novel algorithm simultaneously performing consonant\\/vowel (C\\/V) segmentation and pitch detection is proposed. Based on this algorithm, a consonant enhancement method and a hierarchical neural network scheme are explored for Mandarin speech recognition. As a result, an improvement of 12% in consonant recognition rate is obtained and the number of recognition candidates is reduced from 1300 to 63. A series

  9. High Efficiency Somatic Embrogenesis and Plant Regeneration in Suspension Cultures of an Ornamental Ginger Hybrid (Hedychium muluense x cv ‘Starburst’)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plants were successfully regenerated via somatic embryogenesis from shoot apex-derived callus of an ornamental ginger hybrid, Hedychium muluense x cv ‘Starburst’. H. muluense is a dwarf species and ‘Starburst’ is a hybrid cultivar with white and very fragrant flowers in a circular, wheel-like arrang...

  10. Confirmation By QTL mapping Of The Malus Robusta (Cv. Robusta 5) derived powdery mildew resistance gene Pl1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Geneva® apple rootstock breeding program has made extensive use of Malus robusta cv. Robusta 5 as a source of resistance to fire blight. Robusta 5 has also been used as the source of powdery mildew resistance by other breeding programs and a single locus Pl1 has been associated with this resist...

  11. Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg

    E-print Network

    Semiconductor Physics and Quantum Solar Energy Conversion PV-related research at CvO University Oldenburg GRECO Cvo UNI OL/ Nds.PV-Symp. 06-2009 semiconductor physics / quantum solar energy conversion;Semiconductor Physics and Quantum Solar Energy Conversion Semiconductor Physics and Quantum Solar Energy

  12. Influência de fatores químicos do solo sobre a incidência do mal-do-Panamá na bananeira cv. pacovan na Paraíba

    Microsoft Academic Search

    Edson Batista Lopes; Carlos Henrique de Brito; Ivanildo Cavalcanti de Albuquerque; Arlington Ricardo

    This objective of this work was to investigate the correlation of chemicals soil factors and its influences with the incidence and severity of Panama disease ( Fusarium oxysporum f. sp. cubense ) in banana tree ( Musa sapientium cv. pacovan) in the State of Paraíba. The work was carried out at Areia, Alagoa Nova, Bananeiras and Lagoa Seca Municipalities, where

  13. The influence of fruit ripening on the phytochemical content and biological activity of Capsicum chinense Jacq. cv Habanero

    Microsoft Academic Search

    Federica Menichini; Rosa Tundis; Marco Bonesi; Monica R. Loizzo; Filomena Conforti; Giancarlo Statti; Bruno De Cindio; Peter J. Houghton; Francesco Menichini

    2009-01-01

    During the past decade, it has been reported that the consumption of certain foods and spices such as pepper may have a positive effect on health. The present study evaluates the influence of fruit ripening on total phenols, flavonoids, carotenoids and capsaicinoids content and antioxidant, hypoglycaemic and anticholinesterase activities of Capsicum chinense Jacq. cv Habanero. The chemical investigation showed a

  14. Internship Checklist for DEB students 1. Before you submit a CV and cover letter to any company, please have Dr.

    E-print Network

    Ferrara, Katherine W.

    Internship Checklist for DEB students Feb 2008 1. Before you submit a CV and cover letter to any an internship (in Objective section) and also list the DEB as part of your PhD education. Example: PhD in Chemistry with a Designated Emphasis in Biotechnology. 3. If you are offered an internship, please notify Dr

  15. Inherited transgene expression of the uidA and bar genes in Lilium longiflorum cv. Nellie White

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The expression of two transgenes, bar and uidA, was studied in Lilium longiflorum cv. Nellie White plants. ‘Nellie White’ had been transformed using the gene gun to bombard with pDM327 that contains the bar-uidA fusion gene under control of the CaMV 35S promoter. PCR analysis confirmed that eight ...

  16. Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)

    Microsoft Academic Search

    D. V. Lynch; P. L. Steponkus

    1987-01-01

    Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the

  17. The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface.

    PubMed

    Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A; Bianchet, Mario A; Wang, Lai-Xi; Wilson, Iain B H; Vasta, Gerardo R

    2013-08-23

    The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is "hijacked" by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 "self"-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

  18. The Galectin CvGal1 from the Eastern Oyster (Crassostrea virginica) Binds to Blood Group A Oligosaccharides on the Hemocyte Surface*

    PubMed Central

    Feng, Chiguang; Ghosh, Anita; Amin, Mohammed N.; Giomarelli, Barbara; Shridhar, Surekha; Banerjee, Aditi; Fernández-Robledo, José A.; Bianchet, Mario A.; Wang, Lai-Xi; Wilson, Iain B. H.; Vasta, Gerardo R.

    2013-01-01

    The galectin CvGal1 from the eastern oyster (Crassostrea virginica), which possesses four tandemly arrayed carbohydrate recognition domains, was previously shown to display stronger binding to galactosamine and N-acetylgalactosamine relative to d-galactose. CvGal1 expressed by phagocytic cells is “hijacked” by the parasite Perkinsus marinus to enter the host, where it proliferates and causes systemic infection and death. In this study, a detailed glycan array analysis revealed that CvGal1 preferentially recognizes type 2 blood group A oligosaccharides. Homology modeling of the protein and its oligosaccharide ligands supported this preference over type 1 blood group A and B oligosaccharides. The CvGal ligand models were further validated by binding, inhibition, and competitive binding studies of CvGal1 and ABH-specific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and intact hemocytes and by surface plasmon resonance analysis. A parallel glycomic study carried out on oyster hemocytes (Kurz, S., Jin, C., Hykollari, A., Gregorich, D., Giomarelli, B., Vasta, G. R., Wilson, I. B. H., and Paschinger, K. (2013) J. Biol. Chem. 288,) determined the structures of oligosaccharides recognized by CvGal1. Proteomic analysis of the hemocyte glycoproteins identified ?-integrin and dominin as CvGal1 “self”-ligands. Despite strong CvGal1 binding to P. marinus trophozoites, no binding of ABH blood group antibodies was observed. Thus, parasite glycans structurally distinct from the blood group A oligosaccharides on the hemocyte surface may function as potentially effective ligands for CvGal1. We hypothesize that carbohydrate-based mimicry resulting from the host/parasite co-evolution facilitates CvGal1-mediated cross-linking to ?-integrin, located on the hemocyte surface, leading to cell activation, phagocytosis, and host infection. PMID:23824193

  19. ParaSITE

    E-print Network

    Rakowitz, Michael

    1998-01-01

    paraSITE proposes the appropriation of exterior ventilation systems on existing architecture to inflate pneumatic shelters that are designed for homeless people. This project involves the production of a series of inflatable ...

  20. Coventry University College is a trading name of Coventry University College Limited. A company registered in England and Wales under company number 07781274. Registered office: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB

    E-print Network

    Low, Robert

    Street, Coventry, United Kingdom CV1 5FB JOB DESCRIPTION & PERSON SPECIFICATION 1. JOB INFORMATION Post. Registered office: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB 10. To undertake Street, Coventry, United Kingdom CV1 5FB 3. PERSON SPECIFICATION This is a new and exciting role in a new

  1. CvL, a lectin from the marine sponge Cliona varians: Isolation, characterization and its effects on pathogenic bacteria and Leishmania promastigotes.

    PubMed

    Moura, Raniere M; Queiroz, Alexandre F S; Fook, Jacy M S L L; Dias, Anny S F; Monteiro, Norberto K V; Ribeiro, Jannisson K C; Moura, Gioconda E D D; Macedo, Leonardo L P; Santos, Elizeu A; Sales, Maurício P

    2006-12-01

    CvL, a lectin from the marine sponge Cliona varians was purified by acetone fractionation followed by Sepharose CL 4B affinity chromatography. CvL agglutinated papainized treated human erythrocytes with preference for type A erythrocytes. The lectin was strongly inhibited by monosaccharide d-galactose and disaccharide sucrose. CvL is a tetrameric glycoprotein of 28 kDa subunits linked by disulphide bridges with a molecular mass of 106 kDa by SDS-PAGE and 114 kDa by Sephacryl S300 gel filtration. The lectin was Ca2+ dependent, stable up to 60 degrees C for 60 min, with optimum pH of 7.5. CvL displays a cytotoxic effect on gram positive bacteria, such as Bacillus subtilis and Staphylococcus aureus. However, CvL did not affect gram negative bacteria, such as Escherichia coli and Pseudomonas aeruginosa. Leishmania chagasi promastigotes were agglutinated by CvL up to 2(8) titer. These findings are indicative of the physiological defense roles of CvL and its possible use in the antibiosis of bacteria and protozoa pathogenic. PMID:17020812

  2. Concentration and pump intensity dependent gain studies for disodium flourescein (FDS), cresyl violet (CV), and rhodamine- 590 (Cl) and cresyl violet mixture

    NASA Astrophysics Data System (ADS)

    Gaur, Arun; Taneja, L.; Sharma, A. K.; Mohan, D.; Singh, R. D.

    1991-06-01

    The dye concentration dependence gain spectra for disodium fluorescein (FDS) cresyl violet (CV) and a dye mixture [Rh 590 (Cl)+CV] dissolved in methanol have been studied by amplified spontaneous emission (ASE) technique under pulsed N 2-laser excitation. On comparing the efficiency curves, the dominant role of photoquenching (excited state absorption) was clearly observed in the non-rigid dye FDS, whereas it is non-existent in rigid dye CV at the same concentration. In case of a dye mixture, the energy transfer excitation was found to overcome the inner filter effect and other losses. The various energy transfer mechanisms in these dyes have also been discussed.

  3. Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. Cv. MRQ 74)

    PubMed Central

    Taha, Rosna Mat; Saleh, Azani; Mahmad, Noraini; Hasbullah, Nor Azlina; Mohajer, Sadegh

    2012-01-01

    Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5?mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5?mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1?mg/L BAP, and 0.1?mg/L ?-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1?mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds. PMID:22919338

  4. Brassinosteroid stimulation of hypocotyl elongation and wall relaxation in pakchoi (Brassica chinensis cv Lei-Choi)

    SciTech Connect

    Tzannwei Wang; Cosgrove, D.J.; Arteca, R.N. (Pennsylvania State Univ., University Park (United States))

    1993-03-01

    Hypocotyl elongation of pakchoi (Brassica chinensis cv Lei-Choi) was stimulated by applying 300 ng of brassinosteroid (2[alpha],3[alpha],22[beta],23[beta]-tetrahydroxy-24[beta]-methyl-B-homo-7-oxa-5[alpha]-cholestan-6-one, BR) in 1 [mu]L of 50% ethanol to the apex of hypocotyls. BR had its greatest effect on elongation of the apical 3-mm region below the cotyledonary node (75% stimulation) between 6 and 18 h after treatment. Stress/strain (Instron) analysis of this 3-mm region revealed that plastic and elastic components of extension were not significantly different between BR-treated and control seedlings. In pressure-block experiments, the initial rate of relaxation was 2-fold faster in BR-treated plants as compared with controls, whereas after 125 min the total amount of relaxation and the relaxation rate were the same for the two treatments. Osmotic pressure of cell sap expressed from this 3-mm region showed a large decrease (28%) in BR-treated seedlings compared to the controls. The authors conclude that BR stimulates growth in pakchoi by accelerating the biochemical processes that cause wall relaxation, without inducing a large change in wall mechanical properties. 19 refs., 3 figs., 1 tab.

  5. Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers

    SciTech Connect

    Wenling Hsieh; Sze, Heven (Univ. of Maryland, College Park (United States))

    1991-05-01

    The transport properties of Ca-pumping ATPases from carrot (Daucus carota cv. Danvers) tissue culture cells were studied. ATP dependent Ca transport in vesicles that comigrated with an ER marker, was stimulated 3-4 fold by calmodulin. Cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum Ca-ATPase) partially inhibited oxalate-stimulated Ca transport activity; however, it had little or not effect on calmodulin-stimulated Ca uptake. The results suggested the presence of two types of Ca ATPases, and ER- and a plasma membrane-type. Incubation of membranes with (gamma{sup 32}P)ATP resulted in the formation of a single acyl ({sup 32}P) phosphoprotein of 120 kDa. Formation of this phosphoprotein was dependent on Ca, and enhanced by La {sup 3+}, characteristic of the plasma membrane CaATPase. Acidic phospholipids, like phosphatidylserine, stimulated Ca transport, similar to their effect on the erythrocyte plasma membrane CaATPase. These results would indicate that the calmodulin-stimulated Ca transport originated in large part from a plasma membrane-type Ca pump of 120 kDa.

  6. Changes in phenol content during strawberry (Fragariaxananassa, cv. Chandler) callus culture.

    PubMed

    López Arnaldos, Tomás; Muñoz, Romualdo; Ferrer, María Angeles; Calderón, Antonio A.

    2001-11-01

    Total soluble phenols, soluble flavanols, (+)-catechin, ferulic acid and 1-O-feruloyl-beta-d-glucose were analyzed during the development of a strawberry (Fragariaxananassa, cv. Chandler) callus culture. The time-course changes of the different phenols assayed were well correlated with callus growth and morphology. The changes in polyphenol oxidase (EC 1.10.3.1-2) and beta-glucosidase (EC 3.2.1.21) activities in the callus were also examined. The total phenol, soluble flavanols and (+)-catechin contents were high during the preexponential and exponential phases of growth. The subsequent decrease in (+)-catechin concentration coincided with high levels of polyphenol oxidase activity. The 1-O-feruloyl-beta-d-glucose content was highest as callus growth ceased, and its subsequent decrease was accompanied by the increased production of ferulic acid. This increase in ferulic acid was accompanied by an increase in beta-glucosidase activity. The ferulic acid content decreased at the end of culture, when callus growth had stopped and showed clear symptoms of senescence. This decrease in the ferulic acid concentration was accompanied by an increase in the levels of ferulic acid bound to cell wall components. PMID:12060275

  7. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex

    PubMed Central

    Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

    2013-01-01

    Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees’ latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins’ autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458

  8. Chemical and spectroscopic characteristics of the wood of Vitis vinifera cv. Sangiovese affected by esca disease.

    PubMed

    Agrelli, Diana; Amalfitano, Carmine; Conte, Pellegrino; Mugnai, Laura

    2009-12-23

    Chemical and spectroscopic analyses ((13)C cross-polarization-magic angle spinning NMR and attenuated total reflection Fourier transform infrared spectroscopies) were carried out on the wood of Vitis vinifera cv. Sangiovese with brown-red discoloration and black streaks caused by esca disease. The analyses of the brown-red wood revealed the destruction of hemicelluloses and noncrystalline cellulose as well as modifications in the pectic and ligninic wood fractions. The pectic fraction consisted of carbohydrates associated with polyphenols. The lignin fraction exhibited only a few changes in the aromatic systems and a partial demethylation, and it appeared to be associated with condensed phenolic components probably arising from response polyphenols. The degradation of hemicelluloses and noncrystalline cellulose in brown-red wood, where the pathogens Phaeoacremonium aleophilum and Phaeomoniella chlamydospora prevail with respect to the other fungus Fomitiporia mediterranea, was consistent with reports on the degradative activity of such fungi in vitro carried out on model substrates. The observed alterations could also be attributed to the radical oxidation process caused by the oxidative response of defense itself triggered by infection, as suggested by the accumulation of postinfectional compounds. The analyses of wood tissue with black streaks showed less marked deterioration; here, an increase in pectic and phenolic substances, which probably accumulate in the xylem vessels as a response to the infection, was observed. PMID:19919033

  9. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex.

    PubMed

    Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

    2013-03-01

    Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees' latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins' autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458

  10. Regulation of Cytokinin Oxidase Activity in Callus Tissues of Phaseolus vulgaris L. cv Great Northern 1

    PubMed Central

    Chatfield, J. Mark; Armstrong, Donald J.

    1986-01-01

    The regulation of cytokinin oxidase activity in callus tissues of Phaseolus vulgaris L. cv Great Northern has been examined using an assay based on the oxidation of N6-(?2-isopentenyl)adenine-8-14C (i6 Ade-8-14C) to adenine. Solutions of exogenous cytokinins applied directly to the surface of the callus tissues induced relatively rapid increases in cytokinin oxidase activity. The increase in activity was detectable after 1 hour and continued for about 8 hours, reaching values two- to three-fold higher than the controls. The cytokinin-induced increase in cytokinin oxidase activity was inhibited in tissues pretreated with cordycepin or cycloheximide, suggesting that RNA and protein synthesis may be required for the response. Rifampicin and chloramphenicol, at concentrations that inhibited the growth of Great Northern callus tissues, were ineffective in inhibiting the increase in activity. All cytokinin-active compounds tested, including both substrates and nonsubstrates of cytokinin oxidase, were effective in inducing elevated levels of the enzyme in Great Northern callus tissue. The cytokinin-active urea derivative, Thidiazuron, was as effective as any adenine derivative in inducing this response. The addition of Thidiazuron to the reaction volumes used to assay cytokinin oxidase activity resulted in a marked inhibition of the degradation of the labeled i6 Ade-8-14C substrate. On the basis of this result, it is possible that Thidiazuron may serve as a substrate for cytokinin oxidase, but other mechanisms of inhibition have not yet been excluded. PMID:16664650

  11. Visualisation and quantification of CV chondrite petrography using micro-tomography

    NASA Astrophysics Data System (ADS)

    Hezel, Dominik C.; Elangovan, Premkumar; Viehmann, Sebastian; Howard, Lauren; Abel, Richard L.; Armstrong, Robin

    2013-09-01

    Micro-computed tomography is a non-destructive technique that allows the study of 3D meteorite petrography. The technique produces a unique and instructive visualisation of the meteorite for quantifying its components. We studied the overall petrography of the two CV chondrites Allende and Mokoia to constrain their formation histories. A set of movies and stereographic images detail the 3D petrography. Component modal abundances agree with previous reports and modal abundance differences between Allende and Mokoia support the chondrule-matrix complementarity and that chondrules and matrix formed from the same chemical reservoir. We identified two types of chondrules, a normal type and one where a normal type I or II chondrule is almost completely encapsulated by an opaque-rich layer. This layer was probably acquired during a late stage condensation process. The appearance of opaques in chondrules and matrix is different, not supporting a genetic relationships between these. Low abundances of compound chondrules (1.75 vol% in Allende and 2.50 vol% in Mokoia) indicate low chondrule densities and/or low relative component velocities in chondrule formation regions. Porosities on a scale <10-20 ?m allowed for only local aqueous alteration processes on the meteorite parent bodies.

  12. Chelator-enhanced lead accumulation in Agropyron elongatum cv Szarvasi-1 in hydroponic culture.

    PubMed

    Vashegyi, Ildikó; Cseh, Edit; Lévai, László; Fodor, Ferenc

    2011-03-01

    Hydroponic culture was applied to compare the efficiency of K2EDTA and citrate in mobilizing Pb for accumulation in Agropyron elongatum cv. Szarvasi-1 and their effects on some physiological characteristics of the plants. The plants were grown in nutrient solutions containing 0, 10, and 100 microM Pb(NO3)2 combined with chelating agents added to the nutrient solutions after 21 days of growth, in 3 concentrations (0, 100, and 500 microM). The effects were measured after eight days. The energy grass proved to be greatly resistant to the treatments, as was reflected in the slight inhibition of growth, the resistance of the photosynthetic electron transport chain and the chlorophyll composition and the lack of change in the malone-dialdehyde content. Fundamental differences can be identified between the effects of EDTA and citrate. Citrate had only a little effect on the physiological parameters, which may be due to the strongly increasing lead accumulation with increasing concentration of Pb in the nutrient solution. Additionally, citrate ensured a higher biomass yield with higher shoot Pb accumulation compared to EDTA in almost all treatments. Concerning biomass reduction, 10 microM Pb applied together with K2EDTA has the most deleterious effects on energy grass. The effects correlated with the concentration of EDTA. PMID:21598794

  13. Occurrence of metaxenia and false hybrids in Brassica juncea L. cv. Kikarashina × B. napus

    PubMed Central

    Tsuda, Mai; Konagaya, Ken-ichi; Okuzaki, Ayako; Kaneko, Yukio; Tabei, Yutaka

    2011-01-01

    Imported genetically modified (GM) canola (Brassica napus) is approved by Japanese law. Some GM canola varieties have been found around importation sites, and there is public concern that these may have any harmful effects on related species such as reduction of wild relatives. Because B. juncea is distributed throughout Japan and is known to be high crossability with B. napus, it is assumed to be a recipient of B. napus. However, there are few reports for introgression of cross-combination in B. juncea × B. napus. To assess crossability, we artificially pollinated B. juncea with B. napus. After harvesting a large number of progeny seeds, we observed false hybrids and metaxenia of seed coats. Seed coat color was classified into four categories and false hybrids were confirmed by morphological characteristics and random amplified polymorphic DNA (RAPD) markers. Furthermore, the occurrence of false hybrids was affected by varietal differences in B. napus, whereas that of metaxenia was related to hybridity. Therefore, we suggest that metaxenia can be used as a marker for hybrid identification in B. juncea L. cv. Kikarashina × B. napus. Our results suggest that hybrid productivity in B. juncea × B. napus should not be evaluated by only seed productivity, crossability ought to be assessed the detection of true hybrids. PMID:23136472

  14. The impact of high temperatures on Vitis vinifera cv. Semillon grapevine performance and berry ripening

    PubMed Central

    Greer, Dennis H.; Weedon, Mark M.

    2013-01-01

    The heat event that occurred in many parts of Australia in 2009 was the worst on record for the past decade, with air temperatures exceeding 40°C for 14 days. Our aim was to assess the impacts of this heat event on vine performance, including ripening, yield, and gas exchange of Vitis vinifera cv. Semillon grown in a Riverina vineyard. To assess the affect of high temperatures on Semillon grapevines, the vines were covered with a protective layer to reduce radiant heating and were compared with vines exposed to ambient conditions. The heat event had major effects on ripening; reducing the rate of ripening by 50% and delaying harvest ripeness and causing a high incidence of berry shrivel and sunburn. Yield was not affected. Photosynthesis was reduced 35% by the heat event while transpiration increased nearly threefold and was accounted for by increased stomatal conductance. The conclusion of this study was that heat events delayed ripening in Semillon berries and caused a significant reduction in berry quality. Strategies to minimize the radiant load during heat events are required and this study has confirmed a protective layer can reduce canopy temperatures and enhance berry quality. PMID:24348494

  15. In vitro regeneration in olive (Olea europaea L.) cv, 'Frontio' from nodal segments.

    PubMed

    Mangal, Manisha; Sharma, Dheeraj; Sharma, Mamta; Kumar, Sunil

    2014-09-01

    An efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv 'Frontio' has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurine (BAP), which resulted in 56.2% of bud break and 93.7% survival whereas, a combination of full strength MS medium with 1.0 mg/L each of 3-indole-butyric-acid (IBA) and kinetin was found to be the best for shoot multiplication, in terms of number of shoots (3.6 shoots/explant) and shoot length (2.2 cm). The in vitro shoots were rooted on half-strength MS medium fortified with 0.2 mg/L IBA and 0.2 mg/L alpha-naphthalene acetic acid (NAA) with 1.5 g/L activated charcoal, which supported optimum rooting (60%), with an average of 2-3 roots/shoot, about 2.4 cm length were produced on four weeks of culture. PMID:25241592

  16. Effect of sowing dates and vernalization on Betavulgaris L. cv. Univers C-leaf structure

    PubMed Central

    Sakr, Mohammed M.; Almaghrabi, Omar A.

    2011-01-01

    This research was conducted to study the effect of three different sowing dates (15th October, 15th November and 15th December) and two vernalization treatments (5 °C and ?20 °C) on leaf structure of Betavulgaris L. cv. Univers. The obtained data are summarized as follows: The maximum values of the most studied parameters; lower epidermis + spongy tissue thickness, midrib, mesophyll tissue, vascular bundle, collenchymatous tissue and number of xylem vessels per arm were found as a result of 15th October sowing date treatment compared with the two other sowing dates. Furthermore, effect of the cooling treatments varied according to the recorded character, sowing date and cooling degree. Most of the vernalization treatments at early sowing dates increased the mesophyll tissue, midrib, number of vascular bundles per transverse section, vascular bundle thickness and number of xylem arms per transverse section. The two studied cooling treatments at 15th October sowing date increased both stomatal index and average number of stomata: average number of epidermis cells compared with the control. Furthermore, 15th October under ?20 °C treatment led to small epidermal cells and stomata formation, straight epidermal cell walls and closed stomata in comparison to the control. PMID:23961134

  17. Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)

    PubMed Central

    Sharma, Arishya; Ng, Tzi Bun; Wong, Jack Ho; Lin, Peng

    2009-01-01

    A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80°C. The lectin potently suppressed proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 ?M, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 ?M. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin. PMID:19343172

  18. Promotion of Flowering in Brassica campestris L. cv Ceres by Sucrose

    PubMed Central

    Friend, Douglas J. C.; Bodson, Monique; Bernier, Georges

    1984-01-01

    Flower initiation of the quantitative long-day plant Brassica campestris cv Ceres was earlier and at a lower final leaf number when sucrose was added to the medium in which plants were grown in sterile culture. The optimal concentration of sucrose was 40 to 80 millimolar. This flower-promoting effect of sucrose was not osmotic, as mannitol, sodium chloride, and polyethylene glycol were not effective at equal osmotic potentials. Seedlings grown heterotrophically after treatment with 4-chloro-5-(dimethylamino)-2-phenyl-3-(2H)-pyridazinone to prevent chlorophyll accumulation were also induced to form flower primordia earlier as the sucrose concentration in the medium was increased up to 80 millimolar. Inclusion of 4 millimolar sodium nitrate in the culture medium of green plants did not reduce the flower-promoting effects of sucrose but delayed initiation in plants grown without added sucrose. Removal of CO2 during a single main or supplementary light period, or both, greatly reduced flower initiation. It is concluded that sucrose may be an important controlling factor determining floral initiation in Brassica. PMID:16663739

  19. Perianth bottom-specific blue color development in Tulip cv. Murasakizuisho requires ferric ions.

    PubMed

    Shoji, Kazuaki; Miki, Naoko; Nakajima, Noriyuki; Momonoi, Kazumi; Kato, Chiharu; Yoshida, Kumi

    2007-02-01

    The entire flower of Tulipa gesneriana cv. Murasakizuisho is purple, except the bottom, which is blue. To elucidate the mechanism of the different color development in the same petal, we prepared protoplasts from the purple and blue epidermal regions and measured the flavonoid composition by HPLC, the vacuolar pH by a proton-selective microelectrode, and element contents by the inductively coupled plasma (ICP) method. Chemical analyses revealed that the anthocyanin and flavonol compositions in both purple and blue colored protoplasts were the same; delphinidin 3-O-rutinoside (1) and major three flavonol glycosides, manghaslin (2), rutin (3) and mauritianin (4). The vacuolar pH values of the purple and blue protoplasts were 5.5 and 5.6, respectively, without any significant difference. However, the Fe(3+) content in the blue protoplast was approximately 9.5 mM, which was 25 times higher than that in the purple protoplasts. We could reproduce the purple solution by mixing 1 with two equimolar concentrations of flavonol with lambda(vismax) = 539 nm, which was identical to that of the purple protoplasts. Furthermore, addition of Fe(3+) to the mixture of 1-4 gave the blue solution with lambda(vismax) = 615 nm identical to that of the blue protoplasts. We have established that Fe(3+) is essential for blue color development in the tulip. PMID:17179184

  20. Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.

    PubMed

    Lu, Xu; Zhou, Wei; Gao, Feng

    2009-09-01

    Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome. PMID:19005777

  1. Case study of chondrule alteration with IR spectroscopy in NWA 2086 CV3 meteorite

    NASA Astrophysics Data System (ADS)

    Kereszturi, A.; Gyollai, I.; Szabó, M.

    2015-02-01

    Analyzing the alteration in an olivine chondrule of the NWA 2086 CV3 meteorite, infrared spectral, electron microprobe and optical microscopic observations were correlated to each other. The intensity and wavelength positions of olivine peaks changed characteristically with the progression of alteration and related Fe/Mg substitution inward of the chondrule. Moderate to good correlations were identified between Fo% composition and positions of 830 and 860 cm-1 IR peaks. The disappearance of 1020 cm-1 peak by structural change happens already at a low level alteration without changing the optical appearance of the mineral. The existence of the 980 cm-1 peak is found to be an indicator of the intact phase of olivine. While profiles perpendicular to the chondrule's perimeter showed that the alteration progressed 15-20 ?m distance inward without observable fractures (probablly by some diffusion related process), the "alteration distance" from various obvious fractures inside the chondrule was only 3-5 ?m distance. These observations suggest that the substitution was more effective close to the matrix, and also related to some fluids that although were able to circulate along the large internal fractures too, did not produce such strong substitution there, like what happened close to the matrix. It was also demonstrated that the poorly exploited contact mode observations with ATR based reflection method in infrared spectroscopy provide a useful tool to analyze the alteration at micrometer scale without much sample preparation, and enable identifying alterations already at such a low level where the olivines still look optically intact.

  2. Air-sampling inlet contamination by aircraft emissions on the NASA CV-990 aircraft

    NASA Technical Reports Server (NTRS)

    Condon, E. P.; Vedder, J. F.

    1984-01-01

    Results of an experimental investigation of the contamination of air sampling inlets by aircraft emissions from the NASA CV-990 research aircraft are presented. This four-engine jet aircraft is a NASA facility used for many different atmospheric and meteorological experiments, as well as for developing spacecraft instrumentation for remote measurements. Our investigations were performed to provide information on which to base the selection of sampling locations for a series of multi-instrument missions for measuring tropospheric trace gases. The major source of contamination is the exhaust from the jet engines, which generate many of the same gases that are of interest in atmospheric chemistry, as well as other gases that may interfere with sampling measurements. The engine exhaust contains these gases in mixing ratios many orders of magnitude greater than those that occur in the clean atmosphere which the missions seek to quantify. Pressurized samples of air were collected simultaneously from a scoop located forward of the engines to represent clean air and from other multiport scoops at various aft positions on the aircraft. The air samples were analyzed in the laboratory by gas chromatography for carbon monoxide, an abundant combustion by-product. Data are presented for various scoop locations under various flight conditions.

  3. An Efficient Agrobacterium-Mediated Transformation of Strawberry cv. Camarosa by a Dual Plasmid System.

    PubMed

    Haddadi, Fatemeh; Aziz, Maheran Abd; Abdullah, Siti Nor Akmar; Tan, Soon Guan; Kamaladini, Hossein

    2015-01-01

    An Agrobacterium-mediated transformation method was applied to introduce the luciferase reporter gene under the control of the CaMV35S promoter in the pGreen0049 binary vector into strawberry cv. Camarosa. The in vitro regeneration system of strawberry leaves to be used in the transformation was optimized using different TDZ concentrations in MS medium. TDZ at 16 µM showed the highest percentage (100%) of shoot formation and the highest mean number of shoots (24) produced per explant. Studies on the effects of different antibiotics, namely timentin, cefotaxime, carbenicillin and ampicillin, on shoot regeneration of strawberry leaf explants showed the best shoot regeneration in the presence of 300 mg/L timentin and 150 mg/L cefotaxime. Assessment of the different factors affecting Agrobacterium mediated-transformation of strawberry with the luciferase gene showed the highest efficiency of putative transformant production (86%) in the treatment with no preculture, bacterial OD600 of 0.6 and the addition of 150 mg/L cefotaxime in the pre-selection and selection media. The presence of the luciferase gene in the plant genome was verified by the luciferase reporter gene assay, nested PCR amplification and dot blot of genomic DNA isolated from the young leaves of each putatively transformed plantlet. PMID:25711423

  4. CV-990 Landing Systems Research Aircraft (LSRA) flight #145 drilling of shuttle tire using Tire Assa

    NASA Technical Reports Server (NTRS)

    1995-01-01

    Created from a 1/16th model of a German World War II tank, the TAV (Tire Assault Vehicle) was an important safety feature for the Convair 990 Landing System Research Aircraft, which tested space shuttle tires. It was imperative to know the extreme conditions the shuttle tires could tolerate at landing without putting the shuttle and its crew at risk. In addition, the CV990 was able to land repeatedly to test the tires. The TAV was built from a kit and modified into a radio controlled, video-equipped machine to drill holes in aircraft test tires that were in imminent danger of exploding because of one or more conditions: high air pressure, high temperatures, and cord wear. An exploding test tire releases energy equivalent to two and one-half sticks of dynamite and can cause severe injuries to anyone within 50 ft. of the explosion, as well as ear injury - possibly permanent hearing loss - to anyone within 100 ft. The degree of danger is also determined by the temperature pressure and cord wear of a test tire. The TAV was developed by David Carrott, a PRC employee under contract to NASA.

  5. In vitro antioxidant activity of banana (Musa spp. ABB cv. Pisang Awak).

    PubMed

    Darsini, D Teepica Priya; Maheshu, V; Vishnupriya, M; Sasikumar, J M

    2012-04-01

    The methanolic extract of Musa ABB cv Pisang Awak was investigated for the polyphenolic contents and antioxidant activity. The total phenol and flavonoid contents of the fruit extract were found to be 120 mg gallic acid equivalents (GAE) and 440 mg quercetin equivalents (QE)/100 g of sample dry weight, respectively. The antioxidant activity of the Pisang Awak methanol extract (PAME) (20-500 microg/ml) was determined using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing capacity, 2-2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) radical cation decolourization and hydroxyl radical scavenging capacity (OH*). The EC50 values of DPPH, ABTS and OH* activities of the PAME and butylated hydroxy toluene (BHT) were found to be 65 and 9 microg/ml, 29 and 6 microg/ml, 36 and 42 microg/ml respectively. The reducing capacity increased with increasing concentration (31.5-1000 mg/ml) of the fruit extract and the activity was comparable with the standard BHT. The high performance thin layer chromatography (HPTLC) analysis of the extract revealed the presence of polyphenols. The strong and positive correlations were obtained between total phenol/flavonoid contents (R2 = 0.693-1.0) and free radical scavenging ability was attributed to the polyphenols as the major antioxidants. PMID:22650010

  6. Jasmonates elicit different sets of stilbenes in Vitis vinifera cv. Negramaro cell cultures.

    PubMed

    Taurino, Marco; Ingrosso, Ilaria; D'amico, Leone; De Domenico, Stefania; Nicoletti, Isabella; Corradini, Danilo; Santino, Angelo; Giovinazzo, Giovanna

    2015-01-01

    The plant phenol trans-resveratrol, which is mainly found in grape, displays a wide range of biological effects. A cell suspension culture was developed from calli of grape leaves of Vitis vinifera cv. Negramaro in order to study the bioproduction of resveratrol. The effects of a number of secondary plant metabolism elicitors, namely chitosan, methyl jasmonate, jasmonic acid, coronatine, and 12-oxo-phytodienoic acid, were tested on this cell suspension culture. The identification and quantification of stilbenes was achieved with high performance liquid chromatography, with both spectrophotometric and mass spectrometric detection. Of the tested elicitors, methyl jasmonate was the most effective in inducing the biosynthesis of approximately 4 mg g(-1) dry weight (about 60 mg L(-1)) of resveratrol. Conversely, 12-oxo-phytodienoic acid, jasmonic acid, and coronatine were able to trigger the synthesis of approximately 20 mg g(-1) dry weight (200-210 mg L(-1)) of viniferins. Taken together, our results show for the first time different modulatory effects of closely-related jasmonates on stilbene biosynthesis. PMID:25674504

  7. Biomonitoring of air pollution with mercury in Croatia by using moss species and CV-AAS.

    PubMed

    Spiri?, Zdravko; Vu?kovi?, Ivana; Stafilov, Traj?e; Kušan, Vladimir; Ba?eva, Katerina

    2014-07-01

    Moss samples from four dominant species (Hypnum cupressiforme, Pleurozium schreberi, Homalothecium sericeum and Brachythecium rutabulum) were collected during the summer and autumn of 2010 from 121 sampling sites evenly distributed over the territory of Croatia. Samples were totally digested by using microwave digestion system, whilst mercury was analysed by using cold vapour atomic absorption spectrometry (CV-AAS). Descriptive statistics were done from analyses of mercury in all moss samples. The content of mercury ranged from 0.010 to 0.145 mg kg(-1) with a median value of 0.043 mg kg(-1). Hg distribution map shows the sites of the country with higher levels of this element. High contents of Hg were found in moss samples collected from the regions of Podravina and Istria as a result of anthropogenic pollution. Comparison of median values and ranges with those found in moss samples in 2006 shows slight reduction of mercury air pollution. When compared to the results obtained from recent studies conducted in Slovenia, Macedonia and especially in Norway-which serves as a reference considering the fact that it is a pristine area-mercury air pollution in Croatia is insignificant. PMID:24733436

  8. Characterization of Fusarium wilt resistant somaclonal variants of banana cv. Rasthali by cDNA-RAPD.

    PubMed

    Ghag, Siddhesh B; Shekhawat, Upendra K S; Ganapathi, Thumballi R

    2014-12-01

    Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is counted among the most destructive diseases of crop plants in India. In the absence of any credible control measure to manage this disease, development of resistant cultivars is the best option. Somaclonal variations arising out of long term in vitro culture of plant tissues is an important source of genetic variability and the selection of somaclones having desired characteristics is a promising strategy to develop plants with improved characters. In the present study, we isolated a group of somaclonal variants of banana cv. Rasthali which showed efficient resistance towards Foc race 1 infection in repeated bioassays. cDNA-RAPD methodology using 96 decamer primers was used to characterize these somaclonal variants. Among the four differentially amplified bands obtained, one mapping to the coding region of a lipoxygenase gene was confirmed to be down regulated in the somaclones as compared to controls by real-time quantitative RT-PCR. Our results correlated well with earlier studies with lipoxygenase mutants in maize wherein reduced expression of lipoxygenase led to enhanced resistance towards Fusarium infection. PMID:25160909

  9. Influence of vine vigor on grape (Vitis vinifera L. Cv. Pinot Noir) and wine proanthocyanidins.

    PubMed

    Cortell, Jessica M; Halbleib, Michael; Gallagher, Andrew V; Righetti, Timothy L; Kennedy, James A

    2005-07-13

    The relationships between variations in grapevine (Vitis vinifera L. cv. Pinot noir) growth and resulting fruit and wine phenolic composition were investigated. The study was conducted in a commercial vineyard consisting of the same clone, rootstock, age, and vineyard management practices. The experimental design involved monitoring soil, vine growth, yield components, and fruit composition (soluble solids, flavan-3-ol monomers, proanthocyanidins, and pigmented polymers) on a georeferenced grid pattern to assess patterns in growth and development. Vine vigor parameters (trunk cross-sectional area, average shoot length, and leaf chlorophyll) were used to delineate zones within both blocks to produce research wines to investigate the vine-fruit-wine continuum. There was no significant influence of vine vigor on the amount of proanthocyanidin per seed and only minimal differences in seed proanthocyanidin composition. However, significant increases were found in skin proanthocyanidin (mg/berry), proportion of (-)-epigallocatechin, average molecular mass of proanthocyanidins, and pigmented polymer content in fruit from zones with a reduction in vine vigor. In the wines produced from low-vigor zones, there was a large increase in the proportion of skin tannin extracted into the wine, whereas little change occurred in seed proanthocyanidin extraction. The level of pigmented polymers and proanthocyanidin molecular mass were higher in wines made from low-vigor fruit compared to wines made from high-vigor fruit, whereas the flavan-3-ol monomer concentration was lower. PMID:15998151

  10. Stable MSAP markers for the distinction of Vitis vinifera cv Pinot noir clones.

    PubMed

    Ocaña, Juan; Walter, Bernard; Schellenbaum, Paul

    2013-11-01

    Grapevine is one of the most economically important fruit crops. Molecular markers have been used to study grapevine diversity. For instance, simple sequence repeats are a powerful tool for identification of grapevine cultivars, while amplified fragment length polymorphisms have shown their usefulness in intra-varietal diversity studies. Other techniques such as sequence-specific amplified polymorphism are based on the presence of mobile elements in the genome, but their detection lies upon their activity. Relevant attention has been drawn toward epigenetic sources of variation. In this study, a set of Vitis vinifera cv Pinot noir clones were analyzed using the methylation-sensitive amplified polymorphism technique with isoschizomers MspI and HpaII. Nine out of fourteen selective primer combinations were informative and generated two types of polymorphic fragments which were categorized as "stable" and "unstable." In total, 23 stable fragments were detected and they discriminated 92.5 % of the studied clones. Detected stable polymorphisms were either common to several clones, restricted to a few clones or unique to a single clone. The identification of these stable epigenetic markers will be useful in clonal diversity studies. We highlight the relevance of stable epigenetic variation in V. vinifera clones and analyze at which level these markers could be applicable for the development of forthright techniques for clonal distinction. PMID:23723045

  11. Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids.

    PubMed

    Kanamoto, Hirosuke; Yamashita, Atsushi; Asao, Hiroshi; Okumura, Satoru; Takase, Hisabumi; Hattori, Masahira; Yokota, Akiho; Tomizawa, Ken-Ichi

    2006-04-01

    Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to approximately 36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants. PMID:16604461

  12. The Effect of the Arabic Syllabic Prosodic Features on the Production of English CV Sequences

    E-print Network

    Dr. Mohammad Anani

    The aim of this research is to investigate the effect of the Arabic minimal prosodic unit (the syllable) on the production of English L2 CV sequences. To study the influence of phonetic context upon a given segment and the overall effect the Arabic syntagmatic features on the Arab learner's ability to reorganize in terms of English patterns, samples of conversational exchanges of some native speakers of adult/child Jordanian Arabic were recorded over a two month period. The syllable patterns that regulate the phonological organization of the conversational exchanges were described. To study the effect of the preceding and following consonants on the adjacent vowel sounds, two sets of consonantal contexts were examined: 'emphatic ' vs. 'non-emphatic'. The objectives of this study are the following: (i) to identify prosodic features which span the internal structure of the Arabic word templates, e.g. the vocalic melody of frontness vs. backness (ii) to explore the Arabic language-specific phonological constraints and to determine their effect on the production of English sound patterns (iii) to study the Arabic templates which are similar in shape to English equivalents, because contrastive linguistics studies, to date, have so far focused on phonological negative interlingual transfer to the effective exclusion of positive interlingual transfer. (vi) to demonstrate how English utterances are restructured so that the constituent syllables follow typical Arabic syllabication.

  13. A New Modification of C-V Flap Technique in Nipple Reconstruction: Rolled Triangular Dermal-Fat Flaps.

    PubMed

    Temiz, Gökhan; Ye?ilo?lu, Nebil; Sirino?lu, Hakan; Sarici, Murat

    2014-11-27

    In this study, a simple modification of the C-V flap technique designed to maintain the neo-nipple projection performed as the last stage of breast reconstruction is described. The technique was used in seventeen patients who were treated with breast reconstruction using the transverse rectus abdominis flap. Except for one patient with a 2.3 mm decrease in nipple projection, the projection of all neo-nipples was preserved at the end of the mean follow-up period of 19.4 months. According to the results of our study, modifying the C-V flap technique by adding rolled triangular dermal-fat flaps improves the long-term maintenance of neo-nipple projection. PMID:25428272

  14. Improved Antioxidant and Anti-inflammatory Potential in Mice Consuming Sour Cherry Juice ( Prunus Cerasus cv. Maraska)

    Microsoft Academic Search

    Ana Šari?; Sandra Sobo?anec; Tihomir Balog; Borka Kuši?; Višnja Šverko; Verica Dragovi?-Uzelac; Branka Levaj; Zrinka ?osi?; Željka Ma?ak Šafranko; Tatjana Marotti

    2009-01-01

    The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and\\u000a anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and\\u000a brain), LPO (liver, brain)

  15. The influence of storage on aroma, soluble solids, acid and colour of sour cherries (Prunus cerasus L.) cv. Stevnsbær

    Microsoft Academic Search

    M. B. Petersen; L. Poll

    1999-01-01

    The present study involved a laboratory scale experiment where the impact of post-harvest storage on the quality of sour\\u000a cherries (Prunus cerasus L.) cv Stevnsb?r was investigated. Cherries were stored for 7 days at temperatures of 2???C, 10???C, 20???C, and 30???C,\\u000a and at 20???C in combination with a 20% CO2 atmosphere. Cherry quality was assessed by analysis of soluble solids,

  16. Identification of culturable and originally non-culturable endophytic bacteria isolated from shoot tip cultures of banana cv. Grand Naine

    Microsoft Academic Search

    Pious Thomas; Ganiga K. Swarna; Pulak K. Roy; Prakash Patil

    2008-01-01

    In this article we describe the identification of endophytic bacteria belonging to three groups isolated from shoot tip cultures\\u000a of banana cv. Grand Naine in a recent study (Thomas et al. 2008) based on partial 16S rRNA gene sequence homology analysis. The first group included banana stocks that displayed obvious\\u000a colony growth on MS based tissue culture medium during the first

  17. Preliminary research on conversion of encapsulated somatic embryos of Citrus reticulata Blanco, cv. Mandarino Tardivo di Ciaculli

    Microsoft Academic Search

    Germanà Maria Antonietta; Hafiz Ishfaq Ahmad; Micheli Maurizio; Standardi Alvaro

    2007-01-01

    Somatic embryogenesis was obtained through anther culture of Citrus reticulata, cv. Mandarino Tardivo di Ciaculli. The work was carried out to evaluate the response of somatic embryos inside a sodium\\u000a alginate coating to different storage periods, and to the effects of the germicide PPM (1 ml l?1) and the fungicide Thiophanate-methyl (100 mg l?1). The effect of these alone or in combination, added to

  18. Regeneration of plants from alginate-encapsulated somatic embryos of banana cv. Rasthali ( Musa SPP. AAB Group)

    Microsoft Academic Search

    T. R. Ganapathi; L. Srinivas; P. Suprasanna; V. A. Bapat

    2001-01-01

    Summary  Somatic embryos of banana cv. Rasthali (AAB genomic group) were encapsulated in 5% sodium alginate to produce synthetic seeds.\\u000a The frequency of germination of ecapsulated embryos varied considerably on different gel matrices and substrates used for\\u000a plant development. Maximum conversion frequency of 66% was noted from encapsulated embryos cultured on MS medium. Plantlets\\u000a developed from synthetic seeds were successfully trnasferred

  19. Scheduling drip irrigation for fall tomatoes (Lycopersicon esculentum Mill. cv. Sunny) in the Lower Rio Grande Valley

    E-print Network

    Vargas Prieto, Alberto Miguel

    1985-01-01

    of Advisory Committee: Dr. Grant Vest Two methods of scheduling drip irrigation for tomatoes (Lycopersican esculennJm Mill cv. Sunny) were studied on two soils in Weslaco, Texas during the fall of 1984. Irrigation at 20, 40 or 60% available soil water... Scheduling Evapotranspiration Evaporimeters to Schedule Irrigation Tensiometers in Irrigation Scheduling Precipitation Drip Irrigation Effects of Moisture Regimes on Tomatoes 2 3 5 7 8 9 11 III MATERIALS AND METHODS The Site Treatments...

  20. Respiration and nitrogen fixation of hydroponically cultured Phaseolus vulgaris L. cv. OAC Rico and a supernodulating mutant

    Microsoft Academic Search

    Alexander P. Hansen; Tadakatsu Yoneyama; Hiroshi Kouchi; Peter Martin

    1993-01-01

    This study was undertaken to determine how carbon utilization of fruit production might affect symbiotic activity in hydroponically cultured white bean (Phaseolus vulgaris L.) cv. Rico and its supernodulating genotype R32-BS15 (abbreviated as RBS15). Total plant biomass production of both genotypes was similar. Nodule dry weight of RBS15 consistently scored approximately twice the amount recorded for Rico, while nodule numbers

  1. Gibberellins in developing fruits of Pisum sativum cv. Alaska: Studies on their role in pod growth and seed development

    Microsoft Academic Search

    J. L. Garcia-Martinez; V. M. Sponsel; P. Gaskin

    1987-01-01

    Gibberellins A1, A8, A20 and A29 were identified by capillary gas chromatography-mass spectrometry in the pods and seeds from 5-d-old pollinated ovaries of pea (Pisum sativum cv. Alaska). These gibberellins were also identified in 4-d-old non-developing, parthenocarpic and pollinated ovaries. The level of gibberellin A1 within these ovary types was correlated with pod size. Gibberellin A1, applied to emasculated ovaries

  2. Agrobacterium -mediated transformation of cotton ( Gossypium hirsutum L. cv. Zhongmian 35 ) using glyphosate as a selectable marker

    Microsoft Academic Search

    Fu-Yong Zhao; Yun-Feng Li; Peilin Xu

    2006-01-01

    The most economically significant Chinese cotton cultivar (Gossypium hirsutum L. cv. Zhongmian 35) was transformed via Agrobacterium tumefaciens-mediated DNA transfer. The aroA-M1 gene that confers resistance to the glyphosate was fused with a chloroplast-transit peptide of Arabidopsis thaliana 5-enolpyruvyl-3-phosphoshikimate synthase (ASP) and expressed in cotton plants under the control of a CaMV35S promoter. Transgenic plants were directly selected on medium

  3. Effects of alkalis, oxidants and urea on the nutritive value of rhodes grass ( Chloris gayana cv. Callide)

    Microsoft Academic Search

    B. C Granzin; G. McL Dryden

    2003-01-01

    Two in sacco experiments were conducted to evaluate the impact on the nutritive value of rhodes grass hay (Chloris gayana cv. Callide) of treatment with alkalis or oxidants. In Experiment 1, three alkalis (Ca(OH)2, NaOH, CaO) and two oxidants (NaOCl and H2O2) were applied at levels of 0, 20, 40, 60 or 80g\\/kg of dry matter (DM). NaOH, Ca(OH)2 and

  4. Investigation of the cholesterol-lowering action of insoluble fibre derived from the peel of Citrus sinensis L. cv. Liucheng

    Microsoft Academic Search

    C.-F. Chau; Y.-L. Huang; C.-Y. Lin

    2004-01-01

    A water-insoluble fibre-rich fraction (WIFF) was isolated from the peel of Citrus sinensis L. cv. Liucheng. The effects of a WIFF-containing diet on lipid and cholesterol absorption in hamsters were investigated and compared with those of a cellulose-containing diet and fibre-free diet, as controls. Results demonstrated that WIFF could significantly (P<0.05) decrease the levels of serum triglyceride, serum total cholesterol,

  5. An efficient protoplast-to-plant system for the hybrid ornamental shrub, Weigela × florida cv. Bristol Ruby ( Caprifoliaceae )

    Microsoft Academic Search

    Sergio J. Ochatt

    1993-01-01

    Strategies were developed for the successful isolation of large numbers of highly viable protoplasts from the leaves, stems\\u000a and roots of axenic plants of the hybrid ornamental shrubWeigela florida cv Bristol Ruby. Protoplasts, of all sources, were cultured on different media, leading to the establishment of sustained\\u000a divisions, and coupled with the production of multi-celled (>50 cells) colonies. However, those

  6. OpenCV C# wrapper based video enhancement using different optical flow methods in the super-resolution

    Microsoft Academic Search

    A. Nagy; Z. Vamossy

    2008-01-01

    This article presents a simple method on how to implement a super-resolution based video enhancement technique in .NET using the functions of the OpenCV library. First, we outline the goal of this project and after that, a short review of the steps of super-resolution technique is given. As a part of the discussion about the concrete implementation, firstly the general

  7. The Indirect Keyboard Control System by Using the Gaze Tracing Based on Haar Classifier in OpenCV

    Microsoft Academic Search

    Chang-Zheng Li; Chung-Kyue Kim; Jong-Seung Park

    2009-01-01

    This paper describes on the indirect interface system in which general users assign computer instructions just through the gaze tracing, without mouse or keyboard. We use the Web camera to replace the computer-input system. The face region and the eye region was extracted based on Haar classifier implemented on open source computer vision library (OpenCV). It controls mouse-moving by automatically

  8. Anaerobic n-Alkane Metabolism by a Sulfate-Reducing Bacterium, Desulfatibacillum aliphaticivorans Strain CV2803T

    Microsoft Academic Search

    Cristiana Cravo-Laureau; Vincent Grossi; Danielle Raphel; Robert Matheron; Agnes Hirschler-Rea

    2005-01-01

    The alkane-degrading, sulfate-reducing bacterium Desulfatibacillum aliphaticivorans strain CV2803T, recently isolated from marine sediments, was investigated for n-alkane metabolism. The total cellular fatty acids of this strain had predominantly odd numbers of carbon atoms (C odd) when the strain was grown on a C-odd alkane (pentadecane) and even numbers of carbon atoms (C even) when it was grown on a C-even

  9. Changes in volatile compounds of Habanero chile pepper ( Capsicum chinense Jack. cv. Habanero) at two ripening stages

    Microsoft Academic Search

    Jorge Pino; Enrique Sauri-Duch; Rolando Marbot

    2006-01-01

    The steam volatile components of Yucatan Habanero chile pepper (Capsicum chinense Jack. cv. Habanero) at two ripening stages (green and orange) were analyzed using GC and GC\\/MS. Both samples had several compounds in common. One hundred and two compounds were identified, from which (E)-2-hexenal, hexyl 3-methylbutanoate, (Z)-3-hexenyl 3-methylbutanoate, hexyl pentanoate, 3,3-dimethylcyclohexanol, and hexadecanoic acid were found to be the major

  10. Improved antioxidant and anti-inflammatory potential in mice consuming sour cherry juice (Prunus Cerasus cv. Maraska).

    PubMed

    Sari?, Ana; Sobocanec, Sandra; Balog, Tihomir; Kusi?, Borka; Sverko, Visnja; Dragovi?-Uzelac, Verica; Levaj, Branka; Cosi?, Zrinka; Macak Safranko, Zeljka; Marotti, Tatjana

    2009-12-01

    The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties. PMID:19763832

  11. Self Consistent Simulation of C-V Characterization and Ballistic Performance of Double Gate SOI Flexible-FET Incorporating QM Effects

    E-print Network

    Zubair Al Azim; Nadim Chowdhury; Iftikhar Ahmad Niaz; Md. Hasibul Alam; Imtiaz Ahmed; Quazi D. M. Khosru

    2012-11-16

    Capacitance-Voltage (C-V) & Ballistic Current- Voltage (I-V) characteristics of Double Gate (DG) Silicon-on- Insulator (SOI) Flexible FETs having sub 35nm dimensions are obtained by self-consistent method using coupled Schrodinger- Poisson solver taking into account the quantum mechanical effects. Although, ATLAS simulations to determine current and other short channel effects in this device have been demonstrated in recent literature, C-V & Ballistic I-V characterizations by using self-consistent method are yet to be reported. C-V characteristic of this device is investigated here with the variation of bottom gate voltage. The depletion to accumulation transition point (i.e. Threshold voltage) of the C-V curve should shift in the positive direction when the bottom gate is negatively biased and our simulation results validate this phenomenon. Ballistic performance of this device has also been studied with the variation of top gate voltage.

  12. Asteroid Lightcurve Analysis at Riverland Dingo Observatory (RDO): 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV 26.

    NASA Astrophysics Data System (ADS)

    Hills, Kevin

    2013-01-01

    Lightcurves for five asteroids selected from the Collaborative Asteroid Lightcurve Link (CALL) were obtained at Riverland Dingo Observatory (RDO) from 2012 July-September: 501 Urhixidur, 1897 Hind, 1928 Summa, 6261 Chione, and (68216) 2001 CV26.

  13. Africana Studies 45. . . . . . . . . . . . . . . . . . . American Studies 51. . . . . . . . . . . . . . . . . .

    E-print Network

    Aalberts, Daniel P.

    . . . . . . . . . . . . . . . . Physics 259. . . . . . . . . . . . . . . . . . . . . . . . . Political Economy 264. . . . . . . . . . . . . . . . Political Science 266. . . . . . . . . . . . . . . . . . Psychology 279. . . . . . . . . . Williams-Exeter Programme at Oxford University 329. . . . . . . . . . . . Williams-Mystic Program 338

  14. College of Arts and Letters Africana Studies

    E-print Network

    Gallo, Linda C.

    Sciences Exercise Physiology o Exercise Physiology and Nutritional Sciences- (concurrent) Foods and Nutrition Kinesiology Nutritional Sciences o Nutritional Sciences and Exercise Physiology- (concurrent- (concurrent) o Public Health and Latin American Studies- (concurrent) School of Exercise and Nutritional

  15. Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston 1

    PubMed Central

    Taylor, David C.; Weber, Nikolaus; Barton, Dennis L.; Underhill, Edward W.; Hogge, Lawrence R.; Weselake, Randall J.; Pomeroy, M. Keith

    1991-01-01

    Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived (MD) embryo culture system. TAGs accumulating during embryo development exhibited changes in acyl composition similar to those observed in developing zygotic embryos of the same cv, particularly with respect to erucic and eicosenoic acids. However, MD embryos showed a much higher rate of incorporation of 14C-erucoyl moieties into TAGs in vitro than zygotic embryos. Homogenates of early-late cotyledonary stage MD embryos (14-29 days in culture) were assessed for the ability to incorporate 22:1 and 18:1 (oleoyl) moieties into glycerolipids. In the presence of [1-14C]22:1-coenzyme A (CoA) and various acyl acceptors, including glycerol-3-phosphate (G-3-P), radiolabeled erucoyl moieties were rapidly incorporated into the TAG fraction, but virtually excluded from other Kennedy Pathway intermediates as well as complex polar lipids. This pattern of erucoyl incorporation was unchanged during time course experiments or upon incubation of homogenates with chemicals known to inhibit Kennedy Pathway enzymes. In marked contrast, parallel experiments conducted using [1-14C]18:1-CoA and G-3-P indicated that 14C oleoyl moieties were incorporated into lyso-phosphatidic acids, phosphatidic acids, diacylglycerols, and TAGs of the Kennedy Pathway, as well as other complex polar lipids, such as phosphatidylcholines and phosphatidylethanolamines. When supplied with l-[2-3H(N)]G-3-P and [1-14C]22:1-CoA, the radiolabeled TAG pool contained both isotopes, indicating G-3-P to be a true acceptor of erucoyl moieties. Radio-high-performance liquid chromatography, argentation thin-layer chromatography/gas chromatography-mass spectrometry, and stereospecific analyses of radiolabeled TAGs indicated that 22:1 was selectively incorporated into the sn-3 position by a highly active diacylglycerol acyltransferase (DGAT; EC 2.3.1.20), while oleoyl moieties were inserted into the sn-1 and sn-2 positions. In the presence of sn-1,2-dierucin and [1-14C]22:1-CoA, homogenates and microsomal preparations were able to produce radiolabeled trierucin, a TAG not found endogenously in this species. A 105,000g pellet fraction contained 22:1-CoA:DGAT exhibiting the highest specific activity. The rate of 22:1-CoA:DGAT activity in vitro could more than account for the maximal rate of TAG biosynthesis observed in vivo during embryo development. In double label experiments, G-3-P was shown to stimulate the conversion of [3H]phosphatidylcholines to [3H]diacylglycerols, which subsequently acted as acceptors for 14C erucoyl moieties. In vitro, 22:1 moieties did not enter the sn-1 position of TAGs by a postsynthetic modification or transacylation of preformed TAGs. PMID:16668417

  16. Indicators of aqueous alteration and thermal metamorphism on the CV parent body: Microtextures of a dark inclusion from Allende

    NASA Astrophysics Data System (ADS)

    Kojima, Tomoko; Tomeoka, Kazushige

    1996-07-01

    An unusual dark clast in the Allende CV3 chondrite (termed Allende-AF), which was previously interpreted as a primary aggregate formed in the solar nebula (Kurat et al., 1989; Palme et al., 1989), was re-examined. Our study reveals abundant evidence suggesting that it probably experienced extensive aqueous alteration and subsequent thermal metamorphism on the meteorite parent body. Allende-AF contains numerous rounded to oval-shaped inclusions embedded in a dark matrix. The inclusions, consisting predominantly of fine grains of Fe-rich olivine, have internal textures suggesting that they are pseudomorphs after chondrules. Several inclusions appear to be replaced CAIs. Veins filled with fibrous olivine grains occur abundantly in both inclusions and matrix; some veins (up to 4 mm in length) penetrate several inclusions, providing strong evidence that aqueous alteration occurred after accretion. The fibrous morphology of olivine in veins and inclusions suggests that the olivine was produced by dehydration and thermal transformation of phyllosilicate that had been formed by aqueous alteration. Olivine grains in the matrix contain numerous micro-inclusions of Fe?Ni sulfide, which were probably incorporated during transformation from phyllosilicate. Allende-AF is probably related to the fine-grained variety of dark inclusions reported from CV3 chondrites that has been described as the type containing abundant porous aggregates of Fe-rich olivine by Johnson et al. (1990). Many dark inclusions previously described appear to be similar in texture and mineralogy to Allende-AF, and probably experienced similar secondary process on the meteorite parent body. The wide variation in texture of dark inclusions can be explained by different degrees of aqueous alteration that preceded thermal metamorphism. The size distribution of chondrule pseudomorphs and the abundance of CAI pseudomorphs suggest that the precursor of Allende-AF is a CV type chondrite, probably Allende itself. Oxygen isotopic and chemical compositions are consistent with this interpretation. The CV parent body has been commonly thought to have escaped major secondary processing. However, Allende-AF provides evidence that extensive aqueous alteration and thermal metamorphism have occurred locally on the CV parent body.

  17. How will climate change influence grapevine cv. Tempranillo photosynthesis under different soil textures?

    PubMed

    Leibar, Urtzi; Aizpurua, Ana; Unamunzaga, Olatz; Pascual, Inmaculada; Morales, Fermín

    2015-05-01

    While photosynthetic responses to elevated CO2, elevated temperature, or water availability have previously been reported for grapevine as responses to single stress factors, reports on the combined effect of multiple stress factors are scarce. In the present work, we evaluated effects of simulated climate change [CC; 700 ppm CO2, 28/18 °C, and 33/53 % relative humidity (RH), day/night] versus current conditions (375 ppm CO2, 24/14 °C, and 45/65 % RH), water availability (well-irrigated vs. water deficit), and different types of soil textures (41, 19, and 8 % of soil clay contents) on grapevine (Vitis vinifera L. cv. Tempranillo) photosynthesis. Plants were grown using the fruit-bearing cutting model. CC increased the photosynthetic activity of grapevine plants grown under well-watered conditions, but such beneficial effects of elevated CO2, elevated temperature, and low RH were abolished by water deficit. Under water-deficit conditions, plants subjected to CC conditions had similar photosynthetic rates as those grown under current conditions, despite their higher sub-stomatal CO2 concentrations. As expected, water deficit reduced photosynthetic activity in association with inducing stomatal closure that prevents water loss. Evidence for photosynthetic downregulation under elevated CO2 was observed, with decreases in photosynthetic capacity and leaf N content and increases in the C/N ratio in plants subjected to CC conditions. Soil texture had no marked effects on photosynthesis and did not modify the photosynthetic response to CC and water-deficit conditions. However, in mature well-irrigated plants grown in the soils with the highest sand content, an important decrease in stomatal conductance was observed as well as a slight decrease in the utilization of absorbed light in photosynthetic electron transport (measured as photochemical quenching), possibly related to a low water-retention capacity of these soils even under well-watered conditions. PMID:25786733

  18. Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane 1

    PubMed Central

    Cahoon, Edgar B.; Lynch, Daniel V.

    1991-01-01

    Glucocerebrosides of whole rye (Secale cerale L. cv Puma) leaf and plasma membrane were analyzed using gas chromatography-mass spectrometry and gas chromatography following hydrolysis or as intact molecules purified by reverse-phase high performance liquid chromatography. Fatty acids of acid-hydrolyzed leaf and plasma membrane glucocerebrosides consisted of >98 weight percent saturated and monounsaturated 2-hydroxy fatty acids which contained 16 to 26 carbon atoms. The major fatty acids detected were 2-hydroxynervonic acid (24:1h), 2-hydroxylignoceric acid (24:0h), 2-hydroxyerucic acid (22:1h), and 2-hydroxybehenic acid (22:0h). Long-chain bases of alkaline-hydrolyzed glucocerebrosides consisted primarily of cis-trans isomers of the trihydroxy base 4-hydroxysphingenine (t18:1) and the dihydroxy base sphingadienine (d18:2) with lesser amounts of 4-hydroxysphinganine (t18:0) and isomers of sphingenine (d18:1). Intact, underivatized glucocerebroside molecular species of rye leaf and plasma membrane were separated into more than 30 molecular species using reverse-phase HPLC. The molecular species composition of leaf and plasma membrane were quantitatively and qualitatively similar. The major molecular species was 24:1h-t18:1 which constituted nearly 40 weight percent of leaf and plasma membrane extracts. Several other species including 22:1h-t18:1, 24:1h-t18:1 (isomer), 22:0h-t18:1, 24:1h-d18:2, and 24:0h-t18:1 each comprised 4 to 8% of the total. It is anticipated that the high performance liquid chromatography procedure developed in this study to separate intact, underivatized lipid molecular species will be useful in future studies of the physical properties and biosynthesis of plant glucocerebrosides. PMID:16667981

  19. Erwinia amylovora affects the phenylpropanoid-flavonoid pathway in mature leaves of Pyrus communis cv. Conférence.

    PubMed

    Vrancken, K; Holtappels, M; Schoofs, H; Deckers, T; Treutter, D; Valcke, R

    2013-11-01

    Flavonoids, which are synthesized by the phenylpropanoid-flavonoid pathway, not only contribute to fruit colour and photoprotection, they also may provide antimicrobial and structural components during interaction with micro-organisms. A possible response of this pathway was assessed in both mature and immature leaves of shoots of 2-year-old pear trees cv. Conférence, which were inoculated with the gram-negative bacterium Erwinia amylovora strain SGB 225/12, were mock-inoculated or were left untreated. The phenylpropanoid-flavonoid pathway was analysed by histological studies, by gene expression using RT-qPCR and by HPLC analyses of the metabolites at different time intervals after infection. Transcription patterns of two key genes anthocyanidin reductase (ANR) and chalcone synthase (CHS) related to the phenylpropanoid-flavonoid pathway showed differences between control, mock-inoculated and E. amylovora-inoculated mature leaves, with the strongest reaction 48 h after inoculation. The impact of E. amylovora was also visualised in histological sections, and confirmed by HPLC, as epicatechin -which is produced via ANR- augmented 72 h after inoculation in infected leaf tissue. Besides the effect of treatments, ontogenesis-related differences were found as well. The increase of certain key genes, the rise in epicatechin and the visualisation in several histological sections in this study suggest a non-negligible impact on the phenylpropanoid-flavonoid pathway in Pyrus communis due to inoculation with E. amylovora. In this study, we propose a potential role of this pathway in defence mechanisms, providing a detailed analysis of the response of this system attributable to inoculation with E. amylovora. PMID:23582642

  20. [Effect of CV-2619 (idebenone) on the half-life and hemolysis of red blood cells in stroke-prone spontaneously hypertensive rats (SHRSP)].

    PubMed

    Suno, M; Shibota, M; Nagaoka, A

    1984-10-01

    The effects of CV-2619 on the half-life and hemolysis of red blood cells (RBC) in stroke-prone spontaneously hypertensive rats (SHRSP) were examined. The half-life of RBC in SHRSP was shorter than that in control Wistar Kyoto rats (WKY) and was significantly prolonged in SHRSP kept on a diet containing 0.1% (w/w) of CV-2619 (calculated at 71.0 mg/kg/day): 11.7 +/- 0.4 days in untreated SHRSP (n = 11); 13.8 +/- 0.1 days in treated SHRSP (n = 5, P less than 0.01); and 14.8 +/- 0.5 days in WKY (n = 6). The hemolysis of RBC in salt-loaded SHRSP was accelerated compared with that in WKY. In SHRSP given CV-2619 (20 or 70 mg/kg/day, p.o.) for 2 weeks, the hemolysis was significantly inhibited; the percent hemolysis was 43.9 +/- 0.9% (n = 10) in the control, 39.5 +/- 0.9% (n = 9, P less than 0.01) in the group given 20 mg/kg CV-2619, and 37.1 +/- 0.8% (n = 9, P less than 0.001) in the group given 70 mg/kg CV-2619. These results suggest that the stabilizing effect of CV-2619 on the membrane of RBC is involved in its therapeutic effects in cerebral vascular disorders. PMID:6510844

  1. TESIS DOCTORAL NEGACION CONSTRUCTIVA PARA

    E-print Network

    Alpuente, María

    TESIS DOCTORAL NEGACION CONSTRUCTIVA PARA PROGRAMACION LOGICA-ECUACIONAL Memoria presentada por Mar CONSTRUCTIVA PARA PROGRAMACION LOGICA-ECUACIONAL Tesis Doctoral en Inform#19;atica presentada por Mar#19;#16;a

  2. Mercury Abundances and Isotopic Compositions in the Murchison (CM) and Allende (CV)Carbonaceous Chondrites

    NASA Technical Reports Server (NTRS)

    Lauretta, D. S.; Klaue, B.; Blum, J. D.; Buseck, P. R.

    2001-01-01

    The abundance and isotopic composition of Hg was determined in bulk samples of both the Murchison (CM) and Allende (CV) carbonaceous chondrites using single- and multi-collector inductively coupled plasma mass spectrometry (ICP-MS). The bulk abundances of Hg are 294 6 15 ng/g in Murchison and 30.0 6 1.5 ng/g in Allende. These values are within the range of previous measurements of bulk Hg abundances by neutron activation analysis (NAA). Prior studies suggested that both meteorites contain isotopically anomalous Hg, with d l 96/202Hg values for the anomalous, thermal-release components from bulk samples ranging from 2260 %o to 1440 9/00 in Murchison and from 2620 9/00 to 1540 9/00 in Allende (Jovanovic and Reed, 1976a; 1976b; Kumar and Goel, 1992). Our multi-collector ICP-MS measurements suggest that the relative abundances of all seven stable Hg isotopes in both meteorites are identical to terrestrial values within 0.2 to 0.5 9/00m. On-line thermal-release experiments were performed by coupling a programmable oven with the singlecollector ICP-MS. Powdered aliquots of each meteorite were linearly heated from room temperature to 900 C over twenty-five minutes under an Ar atmosphere to measure the isotopic composition of Hg released fiom the meteorites as a h c t i o n of temperature. In separate experiments, the release profiles of S and Se were determined simultaneously with Hg to constrain the Hg distribution within the meteorites and to evaluate the possibility of Se interferences in previous NAA studies. The Hg-release patterns differ between Allende and Murchison. The Hg-release profile for Allende contains two distinct peaks, at 225" and 343"C, whereas the profile for Murchison has only one peak, at 344 C. No isotopically anomalous Hg was detected in the thermal-release experiments at a precision level of 5 to 30 9/00, depending on the isotope ratio. In both meteorites the Hg peak at ;340"C correlates with a peak in the S-release profile. This correlation suggests that Hg is associated with S-bearing phases and, thus, that HgS is a major Hg-bearing phase in both meteorites. The Hg peak at 225 C for Allende is similar to release patterns of physically adsorbed Hg on silicate and metal grains.

  3. Identification, quantitation and distribution of gibberellins in fruits of Pisum sativum L. cv. Alaska during pod development

    Microsoft Academic Search

    José L. García-Martinez; Cristina Santes; Stephen J. Croker; Peter Hedden

    1991-01-01

    In addition to the previously-reported gibberellins: GA1; GA8, GA20 and GA29 (García-Martínez et al., 1987, Planta 170, 130–137), GA3 and GA19 were identified by combined gas chromatography-mass spectrometry in pods and ovules of 4-d-old pollinated pea (Pisum sativum cv. Alaska) ovaries. Pods contained additionally GA17, GA81 (2a-hydroxy GA20) and GA29-catabolite. The concentrations of GA1, GA3, GA8, GA19, GA20 and GA29

  4. Opaque Mineral Assemblages at Chondrule Boundaries in the Vigarano CV Chondrite: Evidence for Gas-Solid Reactions Following Chondrule Formation

    NASA Technical Reports Server (NTRS)

    Lauretta, Dante S.

    2004-01-01

    Recent studies of opaque minerals in primitive ordinary chondrites suggest that metal grains exposed at chondrule boundaries were corroded when volatile elements recondensed after the transient heating event responsible for chondrule formation. Metal grains at chondrule boundaries in the Bishunpur (LL3.1) chondrite are rimmed by troilite and fayalite. If these layers formed by gas solid reaction, then the composition of the corrosion products can provide information on the chondrule formation environment. Given the broad similarities among chondrules from different chondrite groups, similar scale layers should occur on chondrules in other primitive meteorite groups. Here I report on metal grains at chondrule boundaries in Vigarano (CV3).

  5. [Transformation of embryogenic Calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) mediated by agrobacterium].

    PubMed

    Li, Da-Xu; Zhang, Jie; Zhao, Jian; Zhang, Yi; Li, Li; Liu, Su-Jun; Chen, Fei; Yang, Zhi-Rong

    2006-02-01

    Formation of embryogenic calli of Siberian wildrye grass (Elymus sibiricus L. cv. Chuancao No.2) was induced from mature seeds as explants, and proliferated on MS medium containing 2,4-D 5.0 mg/L and KT 0.05 mg/L. An effective and stable callus regeneration system was established by optimizing the culture conditions (Tables 1, 2 and Fig.2). After the calli were subcultured 8 weeks, selected the whitish-yellow-coloured compact nodular calli that transformed with plasmid pCAMBIA1304 carrying hygromycin resistance gene (hptII) and Pseudomonas pseudoalcaligenes insecticidal protein gene (ppIP), which was mediated by an Agrobacterium strain EHA105. Resistant plants were obtained after hygromycin selection (Figs.3, 4). Some important factors that affect the transformation efficiency were studied, which included selection pressure, time of embryogenic calli proliferation, OD value of Agrobacterium suspension, temperature, medium and time of co-cultivation, and concentration of antibiotics used for suppressing the overgrowth of Agrobacterium in the course of transformation plant regeneration. This research is the first successful genetic transformation of Elymus sibiricus L. cv. Chuancao No.2 mediated by Agrobacterium. PMID:16477130

  6. Interpretation of F106B and CV580 in-flight lightning data and form factor determination

    NASA Technical Reports Server (NTRS)

    Rudolph, T.; Horembala, J.; Eriksen, F. J.; Weigel, H. S.; Elliott, J. R.; Parker, S. L.; Perala, R. A.

    1989-01-01

    Two topics of in-flight aircraft/lightning interaction are addressed. The first is the analysis of measured data from the NASA F106B Thunderstorm Research Aircraft and the CV580 research program run by the FAA and Wright-Patterson Air Force Base. The CV580 data was investigated in a mostly qualitative sense, while the F106B data was subjected to both statistical and quantitative analysis using linear triggered lightning finite difference models. The second main topic is the analysis of field mill data and the calibration of the field mill systems. The calibration of the F106B field mill system was investigated using an improved finite difference model of the aircraft having a spatial resolution of one-quarter meter. The calibration was applied to measured field mill data acquired during the 1985 thunderstorm season. The experimental determination of form factors useful for field mill calibration was also investigated both experimentally and analytically. The experimental effort involved the use of conducting scale models and an electrolytic tank. An analytic technique was developed to aid in the understanding of the experimental results.

  7. Aqueous alteration of the Bali CV3 chondrite: evidence from mineralogy, mineral chemistry, and oxygen isotopic compositions.

    PubMed

    Keller, L P; Thomas, K L; Clayton, R N; Mayeda, T K; DeHart, J M; McKay, D S

    1994-12-01

    A petrographic, geochemical, and oxygen isotopic study of the Bali CV3 carbonaceous chondrite revealed that the meteorite has undergone extensive deformation and aqueous alteration on its parent body. Deformation textures are common and include flattened chondrules, a well-developed foliation, and the presence of distinctive (100) planar defects in olivine. The occurrence of alteration products associated with the planar defects indicates that the deformation features formed prior to the episode of aqueous alteration. The secondary minerals produced during the alteration event include well-crystallized Mg-rich saponite, framboidal magnetite, and Ca-phosphates. The alteration products are not homogeneously distributed throughout the meteorite, but occur in regions adjacent to relatively unaltered material, such as veins of altered material following the foliation. The alteration assemblage formed under oxidizing conditions at relatively low temperatures (<100 degrees C). Altered regions in Bali have higher Na, Ca, and P contents than unaltered regions which suggests that the fluid phase carried significant dissolved solids. Oxygen isotopic compositions for unaltered regions in Bali fall within the field for other CV3 whole-rocks, however, the oxygen isotopic compositions of the heavily altered material lie in the region for the CM and CR chondrites. The heavy-isotope enrichment of the altered regions in Bali suggest alteration conditions similar to those for the petrographic type-2 carbonaceous chondrites. PMID:11539152

  8. Potential for phytoextraction of copper by Sinapis alba and Festuca rubra cv. Merlin grown hydroponically and in vineyard soils.

    PubMed

    Malagoli, Mario; Rossignolo, Virginia; Salvalaggio, Nico; Schiavon, Michela

    2014-03-01

    The extensive use of copper-bearing fungicides in vineyards is responsible for the accumulation of copper (Cu) in soils. Grass species able to accumulate Cu could be cultivated in the vineyard inter-rows for copper phytoextraction. In this study, the capacity of Festuca rubra cv Merlin and Sinapis alba to tolerate and accumulate copper (Cu) was first investigated in a hydroponic system without the interference of soil chemical-physical properties. After the amendment of Cu (5 or 10 mg Cu l-(1)) to nutrient solution, shoot Cu concentration in F. rubra increased up to 108.63 mg Cu kg(-1) DW, more than three times higher than in S. alba (31.56 mg Cu kg(-1) DW). The relationship between Cu concentration in plants and external Cu was dose-dependent and species specific. Results obtained from the hydroponic experiment were confirmed by growing plants in pots containing soil collected from six Italian vineyards. The content of soil organic matter was crucial to enhance Cu tolerance and accumulation in the shoot tissues of both plant species. Although S. alba produced more biomass than F. rubra in most soils, F. rubra accumulated significantly more Cu (up to threefold to fourfold) in the shoots. Given these results, we recommended that F. rubra cv Merlin could be cultivated in the vineyard rows to reduce excess Cu in vineyard soils. PMID:24234763

  9. Description of Gas-Phase Ion/Neutral Interactions in Differential Ion Mobility Spectrometry: CV Prediction Using Calibration Runs

    NASA Astrophysics Data System (ADS)

    Auerbach, David; Aspenleiter, Julia; Volmer, Dietrich A.

    2014-09-01

    Differential ion mobility spectrometry (DMS) coupled to mass spectrometry is increasingly used in both quantitative analyses of biological samples and as a means of removing background interferences for enhanced selectivity and improved quality of mass spectra. However, DMS separation efficiency using dry inert gases often lacks the required selectivity to achieve baseline separation. Polar gas-phase modifiers such as alcohols are therefore frequently employed to improve selectivity via clustering/declustering processes. The choice of an optimal modifier currently relies on trial and error experiments, making method development a tedious activity. It was the goal of this study to establish a means of CV prediction for compounds using a homologous series of alcohols as gas-phase modifiers. This prediction was based on linear regression of compensation voltages of two calibration runs for the alcohols with the lowest and the highest molecular weights and readily available descriptors such as proton affinity and gas phase acidity of the modifier molecules. All experiments were performed on a commercial quadrupole linear ion trap mass spectrometer equipped with a DMS device between electrospray ionization source and entrance quadrupole lens. We evaluated our approach using a homologous series of 4-alkylbenzoic acids and a selection of 23 small molecules of high chemical diversity. Predicted CV values typically deviated from the experimentally determined values by less than 0.5 V. Several test compounds changed their ion mobility behavior for the investigated gas phase modifiers (e.g., from type B to type A) and thus could thus not be evaluated.

  10. Processor core for real time background identification of HD video based on OpenCV Gaussian mixture model algorithm

    NASA Astrophysics Data System (ADS)

    Genovese, Mariangela; Napoli, Ettore

    2013-05-01

    The identification of moving objects is a fundamental step in computer vision processing chains. The development of low cost and lightweight smart cameras steadily increases the request of efficient and high performance circuits able to process high definition video in real time. The paper proposes two processor cores aimed to perform the real time background identification on High Definition (HD, 1920 1080 pixel) video streams. The implemented algorithm is the OpenCV version of the Gaussian Mixture Model (GMM), an high performance probabilistic algorithm for the segmentation of the background that is however computationally intensive and impossible to implement on general purpose CPU with the constraint of real time processing. In the proposed paper, the equations of the OpenCV GMM algorithm are optimized in such a way that a lightweight and low power implementation of the algorithm is obtained. The reported performances are also the result of the use of state of the art truncated binary multipliers and ROM compression techniques for the implementation of the non-linear functions. The first circuit has commercial FPGA devices as a target and provides speed and logic resource occupation that overcome previously proposed implementations. The second circuit is oriented to an ASIC (UMC-90nm) standard cell implementation. Both implementations are able to process more than 60 frames per second in 1080p format, a frame rate compatible with HD television.

  11. In vitro selection and field responses of somaclonal variant plants of rice cv PR113 for drought tolerance

    PubMed Central

    Verma, Dipti; Ansari, Mohammad Wahid; Agrawal, Ganesh Kumar; Rakwal, Randeep; Shukla, Alok; Tuteja, Narendra

    2013-01-01

    Drought is the major environmental stress that limits rice productivity worldwide. In vitro somaclonal variation using different selection agents has been used for crop improvement. Here, rice plants of cv PR113 were selected in vitro on 30, 50 and 70 g L-1 polyethylene glycol 6,000 (PEG). Callus growth, proliferation, calli volume (first and second culture) and plantlet regeneration (third culture) were found to be decreased upto a certain level to acquire tolerance to PEG-induced drought. From the field data, 30 g L-1 PEG lines showed higher vegetative growth (plant height, tiller number, leaf number, shoot weight and root growth) as compared with 50 g L-1 PEG selected somaclone lines under limited irrigation. The yield parameters-panicle length, panicle weight, grains per panicle, 1,000-grain weight, grain yield per plant, harvest index and grain straw ratio were also higher in 30 g L-1 PEG lines as compared with 50 g L-1 PEG lines. The results, therefore indicate that 30 g L-1 PEG selected somaclone lines were more suited than 50 g L-1 PEG selected somaclone lines under stress as compared with WT. The finding suggests that rice cv PR113 somaclones generated on PEG are found to be drought tolerant under field condition with better yield. PMID:23333982

  12. AYUDA PARA INVIDENTES UTILIZANDO TELEFONOS

    E-print Network

    Autonoma de Madrid, Universidad

    ´on, aplicaci´on para m´oviles, c´odigo, base de da- tos,dpi (puntos por pulgada, dots per inch),layout,GPS. III la UAM. #12;Resumen Resumen El proyecto desarrolla aplicaciones para ayuda a invidentes utlizando tel´efonos inteligentes. La aplicaci´on principal est´a desarrollada para aparatos Android. Tiene como objetivo ayudar

  13. Mechanism of the anti-tumour effect of 2,3,5-trimethyl-6-(3-pyridylmethyl) 1,4-benzoquinone (CV-6504).

    PubMed Central

    Hussey, H. J.; Tisdale, M. J.

    1997-01-01

    2,3,5-Trimethyl-6-(3-pyridylmethyl) 1,4-benzoquinone (CV-6504), an inhibitor of 5-lipoxygenase, effectively suppressed growth of the MAC16 tumour in vivo and prevented the accompanying cachexia, when administered daily at a dose of 10 mg kg(-1). There was a reduction in the tumour concentration of linoleic (LA), arachidonic (AA), oleic, stearic and palmitic acid. In order to elucidate the mechanism of the anti-tumour action, the effect of CV-6504 on the metabolism of AA through the 5-, 12- and 15-lipoxygenase pathways has been determined in cell lines sensitive (MAC16, MAC13, MAC26 and Caco-2) and resistant (A549 and DU-145) to CV-6504. Incubation of all cell lines with [3H]AA led to the appearance of [3H]5-, 12- and 15-HETE. Preincubation of MAC16, MAC13, MAC26 and Caco-2 with 10 microM CV-6504 inhibited the conversion of AA to 5-, 12- and 15-HETE, while in A549 and DU-145 cells there was no effect on metabolism through any lipoxygenase pathway. Two other cell lines, MDA-MB-231 and PC-3, sensitive to growth inhibition by CV-6504, are known to require LA for growth, while DU-145, which was insensitive to growth inhibition by CV-6504, showed no growth response to LA. These results suggest that some tumours are dependent on lipoxygenase metabolites of LA and AA for their continual growth, and interference with this pathway produces a specific growth inhibition. PMID:9062405

  14. Safety and immunogenicity of 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Nigerian children

    PubMed Central

    Odusanya, Olumuyiwa O; Kuyinu, Yetunde A; Kehinde, Omolara A; Shafi, Fakrudeen; François, Nancy; Yarzabal, Juan Pablo; Dobbelaere, Kurt; Rüggeberg, Jens U; Borys, Dorota; Schuerman, Lode

    2014-01-01

    In a previous study, 3-dose primary vaccination of Nigerian infants with the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) was immunogenic for vaccine pneumococcal serotypes, with comparable tolerability between PHiD-CV and control groups. In an open-label study (ClinicalTrials.gov, NCT01153893), 68 primed children received a PHiD-CV booster dose co-administered with a diphtheria-tetanus-acellular pertussis (DTPa) booster dose at 15–21 months and 36 children unprimed for pneumococcal vaccination received two PHiD-CV catch-up doses (first dose co-administered with DTPa booster dose) at 15–21 and 17–23 months. Adverse events were recorded and immune responses were measured before and one month after vaccination. In both groups, pain was the most frequent solicited local symptom and fever was the most frequent solicited general symptom after the booster dose and each catch-up dose. Few grade 3 solicited symptoms and no vaccine-related serious adverse events were reported. After booster vaccination, for each vaccine serotype, at least 98.5% of children had an antibody concentration ?0.2 µg/ml and at least 94.0% had an opsonophagocytic activity (OPA) titer ?8. After 2-dose catch-up, for each vaccine serotype, at least 97.1% had an antibody concentration ?0.2 µg/ml, except for serotypes 6B (82.9%) and 23F (88.6%), and at least 91.4% had an OPA titer ?8, except for serotypes 6B (77.4%) and 19F (85.3%). PHiD-CV induced antibody responses against protein D in both groups. In conclusion, PHiD-CV administered to Nigerian toddlers as a booster dose or 2-dose catch-up was well tolerated and immunogenic for vaccine pneumococcal serotypes and protein D. PMID:24356787

  15. Identification and Expression of Two Novel Cytochrome P450 Genes, CYP6CV1 and CYP9A38, in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).

    PubMed

    Chen, Jun; Li, Chuan; Yang, Zhifan

    2015-04-01

    Cnaphalocrocis medinalis Güenée can cause severe losses in rice. Cytochrome P450s play crucial roles in the metabolism of allelochemicals in herbivorous insects. Two novel P450 cDNAs, CYP6CV1 and CYP9A38, were cloned from the midgut of C. medinalis. CYP6CV1 encodes a protein of 500 amino acid residues, while CYP9A38-predicted protein has 531 amino acid residues. Both cDNA-predicted proteins contain the conserved functional domains for all P450s. Phylogenetic analyses showed that CYP6CV1 is grouped in the cluster containing CYP6B members, while CYP9A38 is in the cluster including CYP9 members. However, both clusters are contained in the same higher lineage. Homologous analysis revealed that CYP6CV1 is most similar to CYP6B8, CYP6B7, CYP6B6, CYP6B2, and CYP6B4 with the highest amino acid identity of 41%. CYP9A38 is closest to CYP9A17, CYP9A21, CYP9A20, and CYP9A19 with the highest amino acid identity of 66%. Studies of temporal expression profiles revealed that CYP9A38 showed a steady increase in mRNA level during the five instar stages, but a low-expression level in pupae, and then presented at a high-expression level again in adults. Similar expression patterns were obtained with CYP6CV1. In the fifth instar larvae, CYP6CV1 was mainly expressed in midgut and fat bodies, whereas CYP9A38 was mainly expressed in midgut. Expression studies also revealed a 3.20-fold over-expression of CYP6CV1 and 3.54-fold over-expression of CYP9A38 after larval exposure to host rice resistance. Our results suggest that both CYP6CV1 and CYP9A38 may be involved in detoxification of rice phytochemicals. PMID:25896119

  16. The Anatomy and Bulk Composition of CAI Rims in the Vigarano (CV3) Chondrite

    NASA Astrophysics Data System (ADS)

    Ruzicka, A.; Boynton, W. V.

    1993-07-01

    A striking feature of Ca,Al-rich inclusions (CAIs) in chondrites is the presence of mineralogical layers that typically form rim sequences up to 50 micrometers thick [1]. Many ideas regarding the origin of CAI rims have been proposed, but none are entirely satisfactory. The detailed mineralogy and bulk compositions of relatively unaltered CAI rims in the Vigarano (CV3) chondrite described here provide constraints on hypotheses of rim formation. Rim Mineralogy: CAIs in Vigarano consist of melilite (mel)- and spinel (sp)- rich varieties, both of which are rimmed [2]. Around mel-rich objects, the layer sequence is CAI interior --> sp-rich layer (sometimes absent) --> mel/anorthite (anor) layer --> Ti-Al-rich clinopyroxene (Tpx) layer --> Al- diopside (Al-diop) layer --> olivine (ol) +/- Al-diop layer --> host matrix. The sequence around sp-rich objects differs from this in that the mel/anor layer is absent. Both the sp-rich layer around mel-cored CAIs and the cores of sp-rich CAIs in Vigarano are largely comprised of a fine-grained (<=1 micrometer) intergrowth of sp, Tpx, and minor mel and perovskite. These intergrowths are typically so fine grained that little internal texture is discernible. Mixing calculations suggest the presence of ~10 vol% Tpx in the sp-rich layer of two mel-cored CAIs, and the presence of ~35 vol% Tpx within one sp-cored CAI. The mel/anor layer is sometimes monomineralic, consisting of mel alone, or bimineralic, consisting of both mel and anor. Where bimineralic, anor typically occurs in the outer part of the layer. In places, anor (An(sub)99-100) has partially altered to nepheline and voids. Rim mel is systematically less gehlenitic than mel in the CAI interiors, especially compared to mel in the interior adjacent to the rims. The Tpx layer (>2 and up to 15 wt% TiO2) and Al-diop layer (<2 wt% TiO2) are monomineralic and show chemical zoning trends radial to the CAIs. Moving outward, TiO2 and Al2O3 generally decrease, while SiO2 and MgO increase, although Al2O3 shows a small concentration maximum in the Al-diop layer. High-quality EMPA data suggest that Ti^3+/Ti^4+ decreases outward in the Tpx layer, and that Fe^3+ is present in the Al-diop layer, implying that a steep gradient in oxidation state occurs across these two layers. The ol layer is comprised of individually zoned grains (<=5 micrometers across) that have forsteritic cores and thin (<=1 micrometer) rims of more ferrous ol. The ol grains often form triple-grain junctions and occasionally form clusters that are enclosed by Al-diop. The texture of this polycrystalline layer suggests that it formed by the attachment of preexisting ol grains onto the surfaces of CAIs, and the triple-junctions and steep zoning profiles of the ol grains suggest that they were annealed in a short-lived heating event. Bulk Rim Composition: Bulk rim compositions for several mel-rich CAIs were determined by using EMPA traverses across representative portions of the rims. These compositions plot within the sp + forsterite (fo) field of the gehlenite-anorthite-forsterite ternary diagram of Stolper [3], and are unattainable by the igneous crystallization of a mel-rich CAI composition. Moreover, a hypothetical melt with the composition of the rims has a predicted crystallization sequence (sp --> sp + fo --> sp + fo + anor or mel or Tpx) that does not correspond to observed rim sequences. It thus appears that (1) the rim region did not form through crystallization of molten CAIs; and (2) rim layers did not originate solely by the crystallization of a melt layer present on a solid CAI core [4,5]. References: [1] Wark D. A. and Lovering J. F. (1977) Proc. LSC 8th, 95-112. [2] Ruzicka A. and Boynton W. V. (1991) Meteoritics, 26, 390-391. [3] Stolper E. (1982) GCA, 46, 2159-2180. [4] Korina M. I. et al. (1982) LPS XIII, 399- 400. [5] Bunch T. E. and Chang S. (1980) Meteoritics, 15, 270- 271.

  17. Observation of Correlated 41Ca and 26Al in CV3 Hibonites

    NASA Astrophysics Data System (ADS)

    Sahijpal, S.; Srinivasan, G.; Wasserburg, G. J.; Goswami, J. N.

    1995-09-01

    The demonstration of the presence of ^41Ca in the early solar system, based on the observation of excess ^41K in Efremovka CAIs [1,2] has led to several attempts that sought to identify plausible stellar sites and processes that could have produced and subsequently injected a host of short-lived radionuclides(e.g. ^41Ca, ^26Al, ^60Fe, ^53Mn and ^107Pd) into the proto-solar cloud [3,4,5]. Radiogenic excess of ^26Mg (from ^26Al decay) was also found in the Efremovka CAIs where excess ^41K was present [6,7]. This association suggests possible co-production and subsequent injection of these two short-lived nuclides to the solar nebula. We have measured K and Mg isotopic composition in a single mineral phase (hibonite) having contrasting ^26Mg excesses to further substantiate this hypothesis. We have chosen hibonites from two CV3 meteorites, Efremovka and Allende for the present study. The Efremovka hibonite is from a CAI (E50) having a well-behaved Mg-Al isotopic systematics with initial ^26Al/^27Al of (4.87 +/-0.87)x10^-5 [6]. The Allende sample (HAL hibonite) on the other hand has a very low initial ^26Al/^27Al of (5.2 +/-1.7)x10^-8 [8]. We have carried out K and Mg isotopic studies of E50 hibonite and K isotopic study of HAL hibonite using procedures described previously [1,2,6]. Because of the small size of E50 hibonites, K isotopic composition could be measured in only a few hibonite grains, where the contribution from neighbouring K-rich phase(melilite) towards the measured K signal was negligible. In the case of HAL hibonite there is a large variation in K content, and the signal at ^39K is often close to the system background, while the signal at mass 41 is dominated by the [^40Ca^42Ca]6++ interference. The magnitude of this interference was estimated by measuring the [^40Ca^43Ca]^++/^43Ca^+ ratio in terrestrial (Madagascar) hibonite as well as in the HAL hibonite. We obtained a value of (7.3 +/-0.6)x10^-6 which is about four times lower than the corresponding value for pyroxene [1,2]. Definite excess of ^41K has been found in E50 hibonites with Ca/K ratio exceeding 5x10^5. A forced linear fit of the data through the solar system ^41K/^39K ratio (0.072) yields an initial ^41Ca/^40Ca of about 1.4x10^-8, which is close to the value reported for Efremovka pyroxene [1]. The Al-Mg isotopic data for the E50 hibonites reveal ^26Mg excess in them, consistent with the results reported earlier [6]. In contrast, no excess 41K was found (within the limits of our experimental uncertainties) in HAL hibonite even though its Ca/K ratio is much higher than in Efremovka hibonite. Our data provide an upper limit of 3x10^-9 for initial ^41Ca/^40Ca in HAL hibonite. These results and data obtained from studies of other Efremovka CAIs [1,2,6,7] show that : (i) ^41Ca and ^26Al are present at the same level (^41Ca/^40Ca about 1.4x10^-8 and ^26Al/^27Al about 5x10^-5) in several Efremovka CAIs and (ii) ^41Ca is very low or absent when ^26Al is at very low level (HAL hibonite). It therefore appears that ^41Ca and ^26Al are coupled either in their original stellar source(s) or were well mixed in some parcels of interstellar material before they were injected to the solar nebula. The lower initials in HAL hibonite may reflect either a heterogeneity of ^26Al and ^41Ca in the solar nebula (due to variable mixing of the source material with nebular material) or processing of material and/or redistribution of Mg and K isotopes at a later time. Although our data cannot decide between these alternatives, extensive studies of HAL and other refractory objects indicate that many CAIs evolved through multiple events in the early solar system spreading over a significant time interval [e.g. 9-12]. References: [1] Srinivasan G. et al. (1994) Astrophys. J. Lett., 431, L67-L70. [2] Srinivasan G. et al. (1995) GCA, submitted. [3] Wasserburg G. J. et al. (1995) Astrophys. J. Lett., 440, L101-L104. [4] Cameron A. G. W. et al. (1995) Astrophys. J. Lett., in press. [5] Ramaty R et al. (1995) Astrophys. J., submitted. [6] Goswami J. N. and Srinivasan G. (1994) Proc.

  18. Interactions between Mild NaCl Stress and Red Light during Lettuce (Lactuca sativa L. cv Grand Rapids) Seed Germination

    PubMed Central

    Scorer, Keith N.; Epel, B. L.; Waisel, Yoav

    1985-01-01

    The sensitivity of lettuce (Lactuca sativa L. cv Grand Rapids) seeds to red light was reduced by NaCl concentrations which had no effect upon the germination of continuously illuminated seeds. The germination capacity of the seeds was fully restored by increased red light exposures. Indirect evidence indicates that NaCl does not affect the photoconversion of red-absorbing form of phytochrome to the far-red absorbing form of phytochrome. Instead, the increased red light requirements are attributable to increases in the threshold levels of the far-red absorbing form of phytochrome necessary to induce germination and to changes in the slopes of the fluence-response curves. Results also show that the sensitivity of the seeds to NaCl decreased as the time between red light irradiation and the imposition of NaCl stress increased. PMID:16664360

  19. Direct identification and characterization of phenolic compounds from crude extracts of buds and internodes of grapevine (Vitis vinifera cv Merlot).

    PubMed

    Qsaib, Said; Mateus, Nuno; Ikbal, Fatima Ez-zohra; Rifai, Lala Aicha; De Freitas, Victor; Koussa, Tayeb

    2014-11-01

    The crude methanol extracts of latent buds and internodes Vitis vinifera L. cv. Merlot were used for the determination of phenolic compounds by a combination of reverse phase HPLC with diode array detection (HPLC-DAD) and mass spectrometry (LC-MS). This method allowed the identification of 9 phenolic compounds without purification or fractionation. These 9 compounds were divided into three groups: procyanidins, flavonols and stilbenes. Detection by HPLC-DAD at different wave lengths of 280 nm to 320 nm, allowed the estimation of concentrations of those compounds. This method permitted, for the first time, both characterization and quantification of polyphenolic compounds in buds of grapevine. Comparison with the results obtained in internodes showed that quercetin, resveratrol tetramer and ?-viniferin had similar levels in buds and internodes while six other compounds identified had higher levels in buds. PMID:25532283

  20. Induction of high-frequency somatic embryogenesis in geranium (Pelargonium x hortorum Bailey cv Ringo Rose) cotyledonary cultures.

    PubMed

    Murthy, B N; Singh, R P; Saxena, P K

    1996-02-01

    The cv Ringo Rose of hybrid seed geranium (Pelargonium x hortorum Bailey), previously shown to be recalcitrant in culture, produced somatic embryos when cotyledonary explants were cultured on regeneration medium containing thidiazuron (TDZ), forchlorfenuron (CPPU), or a combination of indole-3-acetic acid and N(6) benzylaminopurine (IAA+BAP). Amendment of the basal medium with TDZ (0.5 ?M) was the most effective treatment. Addition of amino acids to the medium promoted the growth of somatic embryos. Retention of the proximal region of the cotyledon was crucial for regeneration, but the removal of the distal 1/3 to 1/2 cotyledon had no significant effect on somatic embryogenesis. Cotyledonary explants formed somatic embryos in higher frequency and much earlier than hypocotyl explants cultured on the same medium. The somatic embryos induced on cotyledonary explants were germinated on basal medium. More than 70% of the somatic embryos were converted into plants and transferred to soil. PMID:24178422

  1. Spectroscopic study of impurities and associated defects in nanodiamonds from Efremovka (CV3) and Orgueil (CI) meteorites

    NASA Astrophysics Data System (ADS)

    Shiryaev, A. A.; Fisenko, A. V.; Vlasov, I. I.; Semjonova, L. F.; Nagel, P.; Schuppler, S.

    2011-06-01

    The results of spectroscopic and structural studies of phase composition and defects in nanodiamonds from Efremovka (CV3) and Orgueil (CI) chondrites indicate that nitrogen atomic environment in meteoritic nanodiamonds (MND) is similar to that observed in synthetic counterparts produced by detonation and by the Chemical Vapor Deposition (CVD)-process. Most of the nitrogen in MND appears to be confined to lattice imperfections, such as crystallite/twin boundaries and other extended defects, while the concentration of nitrogen in the MND lattice is low. It is suggested that the N-rich sub-population of MND grains may have been formed with high growth rates in environments rich in accessible N (i.e., N in atomic form or as weakly bonded compounds). For the first time the silicon-vacancy complex (the "silicon" defect) is observed in MND by photoluminescence spectroscopy.

  2. Anatalline and other methyl jasmonate-inducible nicotine alkaloids from Nicotiana tabacum cv. By-2 cell cultures.

    PubMed

    Häkkinen, Suvi T; Rischer, Heiko; Laakso, Into; Maaheimo, Hannu; Seppänen-Laakso, Tuulikki; Oksman-Caldentey, Kirsi-Marja

    2004-10-01

    Anatalline [2,4-di(3-pyridyl)piperidine] accumulation was shown to be induced by methyl jasmonate in Nicotiana tabacum cv. BY-2 cell cultures. Beside anatabine, anatalline represented the most abundant alkaloid, moreover, it was always present in two isomeric forms occurring always in similar concentrations. Both isomers could be completely separated by GC-MS. For structural analysis, the isolation of both isomers was performed using a semi-preparative HPLC system. The structures of anatalline [cis-2,4-di(3-pyridyl)piperidine] and its stereoisomer trans-2,4-di(3-pyridyl)piperidine were confirmed by MS and 1D and 2D NMR spectral data. The biosynthetic origin of anatalline was studied by feeding alkaloid precursors to BY-2 cell cultures. PMID:15490322

  3. Petrography and classification of Ca, Al-rich and olivine-rich inclusions in the Allende CV3 chondrite

    NASA Technical Reports Server (NTRS)

    Kormacki, A. S.; Wood, J. A.

    1984-01-01

    The results of a detailed, systematic petrographic survey of Ca, Al-rich and olivine-rich inclusions in the Allende CV3 chondrite are reported, and a new classification system based on clearly defined and readily applied petrographic criteria is presented. Most Allende inclusions are aggregates containing one or more of three distinct constituents: (1) rimmed concentric objects enriched in Al- and Ti-rich oxide minerals and various amounts of Ca-rich silicates; (2) porous, 'fine-grained' chaotic material enriched in Ca-rich silicates, especially clinopyroxenes and garnets; and (3) porous, 'fine-grained', mafic inclusion matrix, enriched in olivine, pyroxene, and feldspathoids. Two texturally distinct varieties of inclusions consist primarily of inclusion matrix: unrimmed olivine aggregates and rimmed olivine aggregates. Ca, Al-rich inclusions are classified on the basis of the size and abundance of their constituent concentric objects. Some fundamental relationships among Allende inclusions that previusly have not been emphasized are discussed.

  4. Al-26, Pu-244, Ti-50, REE, and trace element abundances in hibonite grains from CM and CV meteorites

    NASA Technical Reports Server (NTRS)

    Fahey, A. J.; Mckeegan, K. D.; Zinner, E.; Goswami, J. N.

    1987-01-01

    Hibonites from the CM meteorites Murchison, Murray, and Cold Bokkeveld, and hibonites and Ti-rich pyroxene from the CV chondrite Allende are studied. Electron microprobe measurements of major element concentrations and track and ion probe measurements of Mg and Ti isotopic ratios, rare earth elements (REEs), and trace element abundances are analyzed. Correlations between isotopic anomalies in Ti, Al-26, Pu-244, and Mg-26(asterisk) are examined. Ti isotopic anomalies are compared with REE and trace element abundance patterns. Reasons for the lack of Al-26 in the hibonites are investigated and discussed. It is observed that there is no correlation between the Ti isotopic compositions, and the presence of Mg-26(asterisk), Pu-244, and REE and trace element patterns in individual hibonite samples. The data reveal that hibonites are not interstellar dust grains but formed on a short time scale and in localized regions of the early solar system.

  5. Morpho-histological study of banana (Musa spp. cv. Grande Naine [AAA]) cell suspensions during cryopreservation and regeneration.

    PubMed

    Georget, Frédéric; Engelmann, Florent; Domergue, Régis; Cote, François

    2009-01-01

    In this work, a morpho-histological study of banana (Musa spp. cv. Grande Naine [AAA]) embryogenic cell suspensions during cryopreservation and regeneration was performed. It was demonstrated that the regeneration process of somatic embryos originating from cryopreserved cell suspensions was different from that of control cell suspensions. Somatic embryos originating from cryopreserved cell suspensions had a unicellular origin. The regeneration process was modified not only by freezing in liquid nitrogen but also by the plasmolyzing effect of the 0.5 M sucrose solution employed during pretreatment. This result explained the high number of embryonic structures formed on M3 medium, compared with the control. Proembryos blocked at the globular stage could pursue their development when they were plated on new culture medium at a lower density after 30 days of culture on M3 medium. The unicellular origin of somatic embryos produced from cryopreserved cell suspensions offers the prospect of using cryopreservation to select non-chimeral transformed plants. PMID:20309495

  6. Physiological and biochemical changes associated with flower development and senescence in so far unexplored Helleborus orientalis Lam. cv. Olympicus.

    PubMed

    Shahri, Waseem; Tahir, Inayatullah; Islam, Sheikh Tajamul; Bhat, Mushtaq Ahmad

    2011-03-01

    The so far unexplored H. Orientalis cv. Olympicus exhibits a unique pattern of flower senescence, involving re-greening of creamy white petaloid sepals at the later stages. The greenish sepals become photosynthetically competent immediately after pollination and persist until the seeds are set. After the seed set, the entire (green) flower abscises from the plant. Flower development of Helleborus orientalis cv. Olympicus growing in the open was divided into six stages (I-VI) from tight bud stage to the senescent stage. The average life span of an individual flower after it is fully open is about 6 days. Membrane permeability of sepal tissues estimated as electrical conductivity of leachates increased during senescence. The content of sugars and soluble proteins in the sepal tissues increased during flower opening and declined thereafter during senescence. The protease activity increased as the flower progressed towards senescence. From the present study, it becomes evident that decline in the sugar status and elevation in specific protease activity leading to degradation of proteins are the important factors regulating development and senescence in H. orientalis flowers. Although the tissue content of soluble proteins registered an overall quantitative decrease but SDS-PAGE of protein extract from sepal tissues suggested a decrease in the expression of high molecular weight proteins and an increase in low molecular weight proteins during flower development and senescence. At this stage it is not known whether the polypeptides that increased during senescence play an important role in the senescence of Helleborus orientalis flowers. The increase in these polypeptides during flower senescence is of particular interest because they may be linked to flower longevity. Understanding the nature of these proteins can provide new insights into the pathways that execute senescence and the post-transcriptional regulation of senescence in this flower system. PMID:23572993

  7. The genetics of domestication of yardlong bean, Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis

    PubMed Central

    Kongjaimun, Alisa; Kaga, Akito; Tomooka, Norihiko; Somta, Prakit; Vaughan, Duncan A.; Srinives, Peerasak

    2012-01-01

    Background and Aims The genetics of domestication of yardlong bean [Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis] is of particular interest because the genome of this legume has experienced divergent domestication. Initially, cowpea was domesticated from wild cowpea in Africa; in Asia a vegetable form of cowpea, yardlong bean, subsequently evolved from cowpea. Information on the genetics of domestication-related traits would be useful for yardlong bean and cowpea breeding programmes, as well as comparative genome study among members of the genus Vigna. The objectives of this study were to identify quantitative trait loci (QTLs) for domestication-related traits in yardlong bean and compare them with previously reported QTLs in closely related Vigna. Methods Two linkage maps were developed from BC1F1 and F2 populations from the cross between yardlong bean (V. unguiculata ssp. unguiculata cv.-gr. sesquipedalis) accession JP81610 and wild cowpea (V. unguiculata ssp. unguiculata var. spontanea) accession TVnu457. Using these linkage maps, QTLs for 24 domestication-related traits were analysed and mapped. QTLs were detected for traits related to seed, pod, stem and leaf. Key Results Most traits were controlled by between one and 11 QTLs. QTLs for domestication-related traits show co-location on several narrow genomic regions on almost all linkage groups (LGs), but especially on LGs 3, 7, 8 and 11. Major QTLs for sizes of seed, pod, stem and leaf were principally located on LG7. Pleiotropy or close linkage of genes for the traits is suggested in these chromosome regions. Conclusions This is the first report of QTLs for domestication-related traits in yardlong bean. The results provide a foundation for marker-assisted selection of domestication-related QTLs in yardlong bean and enhance understanding of domestication in the genus Vigna. PMID:22419763

  8. In vitro propagation and assessment of the genetic fidelity of Musa acuminata (AAA) cv. Vaibalhla derived from immature male flowers.

    PubMed

    Hrahsel, Lalremsiami; Basu, Adreeja; Sahoo, Lingaraj; Thangjam, Robert

    2014-02-01

    An efficient in vitro propagation method has been developed for the first time for Musa acuminata (AAA) cv. Vaibalhla, an economically important banana cultivar of Mizoram, India. Immature male flowers were used as explants. Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) were used for the regeneration process. Out of different PGR combinations, MS medium supplemented with 2 mg L(-1) 6-benzylaminopurine (BAP) + 0.5 mg L(-1) ?-naphthalene acetic acid (NAA) was optimal for production of white bud-like structures (WBLS). On this medium, explants produced the highest number of buds per explant (4.30). The highest percentage (77.77) and number (3.51) of shoot formation from each explants was observed in MS medium supplemented with 2 mg L(-1) kinetin + 0.5 mg L(-1) NAA. While MS medium supplemented with a combination of 2 mg L(-1) BAP + 0.5 mg L(-1) NAA showed the maximum shoot length (14.44 cm). Rooting efficiency of the shoots was highest in the MS basal medium without any PGRs. The plantlets were hardened successfully in the greenhouse with 96% survival rate. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to assess the genetic stability of in vitro regenerated plantlets of M. acuminata (AAA) cv. Vaibalhla. Eight RAPD and 8 ISSR primers were successfully used for the analysis from the 40 RAPD and 30 ISSR primers screened initially. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. The present standardised protocol will find application in mass production, conservation and genetic transformation studies of this commercially important banana. PMID:24222501

  9. Whole-rock 26Al-26Mg systematics of amoeboid olivine aggregates from the oxidized CV3 carbonaceous chondrite Allende

    NASA Astrophysics Data System (ADS)

    Olsen, M. B.; Krot, A. N.; Larsen, K.; Paton, C.; Wielandt, D.; Schiller, M.; Bizzarro, M.

    2011-11-01

    We report on mineralogy, petrography, and whole-rock 26Al-26Mg systematics of eight amoeboid olivine aggregates (AOAs) from the oxidized CV chondrite Allende. The AOAs consist of forsteritic olivine, opaque nodules, and variable amounts of Ca,Al-rich inclusions (CAIs) of different types, and show evidence for alteration to varying degrees. Melilite and anorthite are replaced by nepheline, sodalite, and grossular; spinel is enriched in FeO; opaque nodules are replaced by Fe,Ni-sulfides, ferroan olivine and Ca,Fe-rich pyroxenes; forsteritic olivine is enriched in FeO and often overgrown by ferroan olivine. The AOAs are surrounded by fine-grained, matrix-like rims composed mainly of ferroan olivine and by a discontinuous layer of Ca,Fe-rich silicates. These observations indicate that AOAs experienced in situ elemental open-system iron-alkali-halogen metasomatic alteration during which Fe, Na, Cl, and Si were introduced, whereas Ca was removed from AOAs and used to form the Ca,Fe-rich silicate rims around AOAs. The whole-rock 26Al-26Mg systematics of the Allende AOAs plot above the isochron of the whole-rock Allende CAIs with a slope of (5.23 ± 0.13) × 10-5 reported by Jacobsen et al. (2008). In contrast, whole-rock 26Al-26Mg isotope systematics of CAIs and AOAs from the reduced CV chondrite Efremovka define a single isochron with a slope of (5.25± 0.01) × 10-5 (Larsen et al. 2011). We infer that the excesses in 26Mg* present in Allende AOAs are due to their late-stage open-system metasomatic alteration. Thus, the 26Al-26Mg isotope systematics of Allende CAIs and AOAs are disturbed by parent body alteration processes, and may not be suitable for high-precision chronology of the early solar system events and processes.

  10. Coventry University College is a trading name of Coventry University College Limited. A company registered in England and Wales under company number 07781274. Registered office: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB

    E-print Network

    Low, Robert

    Street, Coventry, United Kingdom CV1 5FB JOB DESCRIPTION & PERSON SPECIFICATION 1. JOB INFORMATION Post company number 07781274. Registered office: Coventry University, Priory Street, Coventry, United Kingdom: Coventry University, Priory Street, Coventry, United Kingdom CV1 5FB 3. PERSON SPECIFICATION This is a new

  11. Effect of various Rhizobium trifolii strains on nitrogenase (C 2 H 2 ) activity profiles of red clover ( Trifolium pratense cv. Venla)

    Microsoft Academic Search

    Kristina Lindström

    1984-01-01

    The effect of variousRhizobium trifolii strains on the nitrogenase activity profiles of red clover (Trifolium pratense cv. Venla) was studied in greenhouse and field experiments. The nitrogenase activity of nodulated roots or whole plants was measured with the acetylene reduction assay at different stages of plant growth. The mean nitrogenase activity followed plant shoot growth rate from the time when

  12. USE OF REPEATED BRONCHOALVEOLAR LAVAGE IN RABBITS TO ASSESS POLLUTANT-INDUCED LUNG CHANGES IN AN ANIMAL MODEL OF CARDIOVASCULAR (CV) DISEASE.

    EPA Science Inventory

    Animal models of coronary heart disease (e.g., hyperlipidemic rabbits) are being used to investigate epidemiologic associations between higher levels of air pollution and adverse CV consequences. Mechanisms by which pollutant-induced lung or systemic inflammation leads to acute C...

  13. Omega Gliadin Genes Expressed in Triticum Aestivum cv Butte 86: Effects of Post-anthesis Fertilizer on Transcript Accumulation During Grain Development

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The partial coding sequences of omega gliadin genes expressed in developing wheat kernels Triticum aestivum cv Butte 86 were identified in EST databases. Three gene assemblies encode proteins with PQQPFP as the predominant repetitive motif. Of these, two encode proteins with at least one cysteine an...

  14. Free radical scavenging activity and phenolic content in achenes and thalamus from Fragaria chiloensis ssp. chiloensis, F. vesca and F. x ananassa cv. Chandler

    Microsoft Academic Search

    José Cheel; Cristina Theoduloz; Jaime A. Rodríguez; Peter D. S. Caligari; Guillermo Schmeda-Hirschmann

    2007-01-01

    The total phenolic, flavonoid and anthocyanin content of achenes (true fruit) and thalamus (receptacle) from the native South American Fragaria chiloensis ssp. chiloensis (f. patagonica and f. chiloensis), Fragaria vesca and Fragaria x ananassa cv. Chandler was determined by spectrophotometric means. Highest phenolic content was found in F. vesca while lowest content was measured for white strawberry (F. chiloensis ssp.

  15. Effect of potassium on drought resistance of Hibiscus rosa-sinensis cv. Leprechaun: Plant growth, leaf macro- and micronutrient content and root longevity

    Microsoft Academic Search

    Jonathan N. Egilla; Fred T. Davies; Malcolm C. Drew

    2001-01-01

    As competition for the limited water supply available for irrigation of horticultural crops increases, research into crop management practices that enhance drought resistance, plant water-use efficiency and plant growth when water supply is limited has become increasingly essential. This experiment was conducted to determine the effect of potassium (K) nutritional status on the drought resistance of Hibiscus rosa-sinensis L. cv.

  16. Nickolas Themelis: Director, Earth Engineering Center http://www.seas.columbia.edu/earth/szekielda-cv.html[8/19/2009 4:40:26 PM

    E-print Network

    Nickolas Themelis: Director, Earth Engineering Center http://www.seas.columbia.edu/earth/szekielda-cv.html[8/19/2009 4:40:26 PM] The Earth Engineering Center of Columbia University KARL SZEKIELDA (212) 923. Szekielda was PI in the Earth Resources Technology Satellite under Skylab (EREP). Appointed National

  17. Effects of three esca-associated fungi on Vitis vinifera L.: V. Changes in the chemical and biological profile of xylem sap from diseased cv. Sangiovese vines

    Microsoft Academic Search

    Giovanni Bruno; Lorenzo Sparapano

    2007-01-01

    A vineyard of Vitis vinifera cv. Sangiovese was surveyed for incidence of esca and xylem sap collection. Sap samples were collected from healthy vines and from those with dual infection by Phaeomoniella chlamydospora (Pch) and Togninia minima (Tmi) or triple infection by Pch, Tmi and Fomitiporia mediterranea (Fme), during each early spring in a 3-year period (2001–2003). In order to

  18. Electronics Division Technical Note No. 189 File: \\\\EAGLE\\cv-cdl-sis\\Docs\\Rack\\SIS Mixer Bias Supply\\Simulation\\Report2.doc Page 1 of 5

    E-print Network

    Groppi, Christopher

    Electronics Division Technical Note No. 189 File: \\\\EAGLE\\cv-cdl-sis\\Docs\\Rack\\SIS Mixer Bias Supply\\Simulation\\Report2.doc Page 1 of 5 Stability Analysis of SIS Mixer Bias Supply with 1K Ohm Isolation Resistors 29 November 2001 J. Effland Summary This memo analyzes the stability of the CDL's SIS

  19. Differences in Stylet Penetration Behaviors of Glassy-winged Sharpshooters on Xylella-Resistant Vitis candicans vs. Susceptible Vitis vinifera cv. ‘Chardonnay’

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Electrical penetration graph (EPG) monitoring was used to compare stylet penetration behaviors of glassy-winged sharpshooter (GWSS), a vector of Xylella fastidiosa (Xf), on Xf-resistant Vitis candicans grape vs. susceptible V. vinifera cv. ‘Chardonnay.’ Frequency of occurrence of X waves (represent...

  20. Effects of pH, sample size, and solvent partitioning on recovery of soluble phenolic acids and isoflavonoids in red clover (Trifolium pratense cv. Kenland)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Several extraction parameters were tested to determine optimal conditions for extracting phenolics from red clover (Trifolium pratense L. cv. Kenland). HPLC-UV profiles were compared before and after partitioning a methanolic extract of soluble phenolics with ethyl acetate-ether (1:1, v/v). The e...

  1. CHANGES IN THE STRATIFICATION AND SPATIAL DISTRIBUTION OF THE BANANA (MUSA AAA CV. GRAND NAINE) ROOT SYSTEM OF POOR, REGULAR, AND GOOD DEVELOPED PLANTS

    Microsoft Academic Search

    M. Araya; F. Blanco

    2001-01-01

    In an attempt to explain the difference in banana (Musa AAA cv. Grand Naine) plants with poor, regular, and good development, their root stratification and spatial distribution was determined in Costa Rican field conditions. Samples were taken from soil blocks at 0 to 30, 30 to 60, and 60 to 90 cm distances from the pseudostem, and at every 15

  2. Survey of 42,000 Gossypium hirsutum cv. Maxxa BAC-End Sequences and Frequency, Type, and Annotation of BAC-derived SSRs.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The quest for more molecular markers is a major initiative in cotton, which lags behind crops such as soybean, maize, and rice in this type of research. In an effort to increase the number of microsatellite markers in Gossypium, BAC-end sequences from a publicly available Gossypium hirsutum cv. Maxx...

  3. Draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi).

    PubMed

    Kolton, Max; Green, Stefan J; Harel, Yael Meller; Sela, Noa; Elad, Yigal; Cytryn, Eddie

    2012-10-01

    Here we report the draft genome sequence of Flavobacterium sp. strain F52, isolated from the rhizosphere of bell pepper (Capsicum annuum L. cv. Maccabi). Flavobacterium spp. are ubiquitous in the rhizospheres of agricultural crops; however, little is known about their physiology. To our knowledge, this is the first published genome of a root-associated Flavobacterium strain. PMID:22965088

  4. A large plasmid isolated from Rhizobium huakuii bv. renge that includes genes for both nodulation of Astragalus sinicus cv. Japan and nitrogen fixation

    Microsoft Academic Search

    Yong Xu; Yoshikatsu Murooka

    1995-01-01

    Rhizobium huakuii bv. renge, which is able to produce nodules on Astragalus sinicus cv. Japan, has been assigned to a new biovariety based on its host range and taxonomic characteristics. We reproducibly demonstrated the presence of an indigenous large plasmid, with a molecular mass of about 260 Mda, in R. huakuii bv. renge and named the plasmid pRhYM. Using a

  5. A new immuno- dystrophin-deficient model, the NSG-mdx4Cv mouse, provides evidence for functional improvement following allogeneic satellite cell transplantation

    PubMed Central

    Arpke, Robert W.; Darabi, Radbod; Mader, Tara L.; Zhang, Yu; Toyama, Akira; Lonetree, Cara-lin; Nash, Nardina; Lowe, Dawn A.; Perlingeiro, Rita C.R.; Kyba, Michael

    2013-01-01

    Transplantation of a myogenic cell population into an immunodeficient recipient is an excellent way of assessing the in vivo muscle-generating capacity of that cell population. To facilitate both allogeneic and xenogeneic transplantations of muscle-forming cells in mice we have developed a novel immunodeficient muscular dystrophy model, the NSG-mdx4Cv mouse. The IL2Rg mutation, which is linked to the Dmd gene on the X chromosome, simultaneously depletes NK cells and suppresses thymic lymphomas, issues that limit the utility of the SCID/mdx model. The NSG-mdx4Cv mouse presents a muscular dystrophy of similar severity to the conventional mdx mouse. We show that this animal supports robust engraftment of both pig and dog muscle mononuclear cells. The question of whether satellite cells prospectively isolated by flow cytometry can confer a functional benefit upon transplantation has been controversial. Using allogeneic Pax7-ZsGreen donors and NSG-mdx4Cv recipients, we demonstrate definitively that as few as 900 FACS-isolated satellite cells can provide functional regeneration in vivo, in the form of an increased mean maximal force-generation capacity in cell-transplanted muscles, compared to a sham-injected control group. These studies highlight the potency of satellite cells to improve muscle function, and the utility of the NSG-mdx4Cv model for studies on muscle regeneration and Duchenne muscular dystrophy therapy. PMID:23606600

  6. arXiv:0807.1594v1[math.CV]10Jul2008 EVOLUTION FAMILIES AND THE LOEWNER EQUATION I: THE

    E-print Network

    Contreras, Manuel D.

    arXiv:0807.1594v1[math.CV]10Jul2008 EVOLUTION FAMILIES AND THE LOEWNER EQUATION I: THE UNIT DISC a general version of the Loewner differential equation which allows us to present a new and unified correspondence with solutions to this new type of Loewner equations. Also, we give a Berkson-Porta type formula

  7. A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV Hollot2, J Horowitz3, and RP Shrestha4, Y Chait5

    E-print Network

    Massachusetts at Amherst, University of

    A New Measure of Patient Responsiveness for Improving Anemia Management Protocols MJ Germain1, CV on performance of anemia management protocols. · Anemia of end-stage kidney disease (ESRD) is characterized-specific gains should play a role in their design. · The interaction of anemia management protocols (AMPs

  8. Feifei Pan CV--Last updated on October 22, 2014 Page 1 of 10 Department of Geography, University of North Texas, Denton, TX 76203

    E-print Network

    Pan, Feifei

    ., Zhang D., Yang N., Pan Z., and Wei Y., 2014: Spatial analysis of climate change in Inner Mongolia during/215 in Environmental Sciences, G=0]. [p] Nie S., Wang C., Li G., Pan F., Xi X., and Luo S., 2014: A signal to noiseFeifei Pan CV--Last updated on October 22, 2014 Page 1 of 10 Feifei Pan Department of Geography

  9. Mutation breeding of banana cv. Highgate ( Musa spp., AAA Group) for tolerance to Fusarium oxysporum f. sp. cubense using chemical mutagens

    Microsoft Academic Search

    B. Bhagwat; E. J. Duncan

    1998-01-01

    Shoot apices of in vitro-grown cultures of banana (Musa spp., AAA Group cv. Highgate) were treated with various concentrations of the mutagens sodium azide, diethyl sulphate, and ethyl methanesulphonate to evaluate their effectiveness in inducing mutations and also with the aim of producing variants tolerant to the fungus Fusarium oxysporum f. sp. cubense. This fungus causes fusarial wilt or Panama

  10. A new immuno-, dystrophin-deficient model, the NSG-mdx(4Cv) mouse, provides evidence for functional improvement following allogeneic satellite cell transplantation.

    PubMed

    Arpke, Robert W; Darabi, Radbod; Mader, Tara L; Zhang, Yu; Toyama, Akira; Lonetree, Cara-Lin; Nash, Nardina; Lowe, Dawn A; Perlingeiro, Rita C R; Kyba, Michael

    2013-08-01

    Transplantation of a myogenic cell population into an immunodeficient recipient is an excellent way of assessing the in vivo muscle-generating capacity of that cell population. To facilitate both allogeneic and xenogeneic transplantations of muscle-forming cells in mice, we have developed a novel immunodeficient muscular dystrophy model, the NSG-mdx(4Cv) mouse. The IL2Rg mutation, which is linked to the Dmd gene on the X chromosome, simultaneously depletes NK cells and suppresses thymic lymphomas, issues that limit the utility of the SCID/mdx model. The NSG-mdx(4Cv) mouse presents a muscular dystrophy of similar severity to the conventional mdx mouse. We show that this animal supports robust engraftment of both pig and dog muscle mononuclear cells. The question of whether satellite cells prospectively isolated by flow cytometry can confer a functional benefit upon transplantation has been controversial. Using allogeneic Pax7-ZsGreen donors and NSG-mdx(4Cv) recipients, we demonstrate definitively that as few as 900 FACS-isolated satellite cells can provide functional regeneration in vivo, in the form of an increased mean maximal force-generation capacity in cell-transplanted muscles, compared to a sham-injected control group. These studies highlight the potency of satellite cells to improve muscle function and the utility of the NSG-mdx(4Cv) model for studies on muscle regeneration and Duchenne muscular dystrophy therapy. PMID:23606600

  11. Physical Analysis of the Complex Rye (Secale cereale L.) Alt4 Aluminium (Aluminum) Tolerance Locus Using a Whole-Genome BAC Library of Rye cv. Blanco

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rye is a diploid crop species with many outstanding qualities, and is also important as a source of new traits for wheat and triticale improvement. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies. The library provides a 6 × genome ...

  12. L'aptitude au croisement avec le seigle (Secale cereale L.) d'une ligne hexaplode de Triticum (cv. Roazon rsul-

    E-print Network

    Paris-Sud XI, Université de

    -clés additionnels : Blé, Triticum aestivum, Trsticale, Gènes d'incompatibilité, lntrogression. SUMMARY Crossability. Additional key-words : Wheat, Triticum aestivum, Triticale, Cross-compatibility, Crossability genesL'aptitude au croisement avec le seigle (Secale cereale L.) d'une lignée hexaploïde de Triticum (cv

  13. [Effects of idebenone (CV-2619) on neurological deficits, local cerebral blood flow, and energy metabolism in rats with experimental cerebral ischemia].

    PubMed

    Nagaoka, A; Suno, M; Shibota, M; Kakihana, M

    1984-09-01

    Improvement of energy metabolism in ischemic cerebral tissue benefits the therapy of occlusive cerebrovascular lesions. In the present study, the effects of 6-(10-hydroxydecyl)-2, 3-dimethoxy-5-methyl-1, 4-benzoquinone (idebenone, CV-2619) on neurological signs, local cerebral blood flow, and cerebral energy metabolism were assessed in stroke-prone spontaneously hypertensive rats (SHRSP) with bilateral carotid artery occlusion (BCAO). Pretreatment with CV-2619 (10-100 mg/kg, p.o.) for three or ten successive days delayed the onset of ischemic seizure (acute stroke) and prolonged survival time in the SHRSP. When the compound (100 mg/kg, i.p.) was given once 30 min after BCAO, it exerted similar ameliorating effects on the neurological deficits. When CV-2619 (100 mg/kg for 3 days) was given orally, it did not inhibit a decrease in regional cerebral blood flow induced by the carotid artery occlusion. However, the same treatment markedly inhibited increases in lactate content and lactate/pyruvate ratio and a decrease in ATP content in the cerebral cortex. In addition, the compound showed no effect on cerebral blood flow in normal rats. These results suggest that CV-2619 has an ameliorating effect on neurological deficits related with cerebral ischemia, and this effect is mediated by improved cerebral energy metabolism. PMID:6500404

  14. Chemical Composition of the Essential Oil Isolated from Wild Catnip Nepeta cataria L. cv. citriodora from the Drôme Region of France

    Microsoft Academic Search

    Jean-Claude Chalchat; Jacques Lamy

    1997-01-01

    Nepeta cataria L. cv. citriodora growing wild in the Drôme region of France was brought into cultivation. Oils produced from cultivated plants harvested throughout the growing season were analyzed by GC and GC\\/MS. Although 42 components were identified, the oil composition did not depend on the time of harvesting or storage of the plant material prior to distillation. The oil

  15. Long-Term Fungal Inhibitory Activity of Water-Soluble Extracts of Phaseolus vulgaris cv. Pinto and Sourdough Lactic Acid Bacteria during Bread Storage?

    PubMed Central

    Coda, Rossana; Rizzello, Carlo G.; Nigro, Franco; De Angelis, Maria; Arnault, Philip; Gobbetti, Marco

    2008-01-01

    The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts of L. brevis AM7 sourdough and P. vulgaris cv. Pinto had a MIC of 40 mg of peptide/ml and 30.9 mg of protein/ml, respectively. MICs were markedly lower when chemically synthesized peptides or partially purified protein fractions were used. The water-soluble extract of P. vulgaris cv. Pinto showed inhibition toward a large number of fungal species isolated from bakeries. Phaseolin alpha-type precursor, phaseolin, and erythroagglutinating phytohemagglutinin precursor were identified in the water-soluble extract of P. vulgaris cv. Pinto by nano liquid chromatography-electrospray ionization-tandem mass spectrometry. When the antifungal activity was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all three proteins were inhibitory. A mixture of eight peptides was identified from the water-soluble extract of sourdough L. brevis AM7, and five of these exhibited inhibitory activity. Bread was made at the pilot plant scale by sourdough fermentation with L. brevis AM7 and addition of the water-soluble extract (27%, vol/wt; 5 mg of protein/ml) of P. vulgaris cv. Pinto. Slices of bread packed in polyethylene bags did not show contamination by fungi until at least 21 days of storage at room temperature, a level of protection comparable to that afforded by 0.3% (wt/wt) calcium propionate. PMID:18849463

  16. Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability

    PubMed Central

    2013-01-01

    Background Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation. Reviewer This article was reviewed by Sándor Pongor, L. Aravind and Anthony M. Poole. PMID:23324115

  17. Tomografía computarizada (TC) y exploraciones para cáncer

    Cancer.gov

    Hoja informativa que describe el procedimiento y la tecnología de exploraciones con tomografía computarizada así como sus usos para el diagnóstico, para exámenes de detección y para tratamiento del cáncer.

  18. Pruebas genéticas para síndromes hereditarios de cáncer

    Cancer.gov

    Hoja informativa sobre pruebas genéticas para riesgos heredados de cáncer; incluye tipos de pruebas, para quién están indicadas y explicación y confidencialidad de los resultados. Ofrece información de pruebas genéticas a domicilio o pruebas para el consumidor.

  19. Transport of Ascorbic and Dehydroascorbic Acids across Protoplast and Vacuole Membranes Isolated from Barley (Hordeum vulgare L. cv Gerbel) Leaves.

    PubMed Central

    Rautenkranz, AAF.; Li, L.; Machler, F.; Martinoia, E.; Oertli, J. J.

    1994-01-01

    Protoplasts, vacuoles, and chloroplasts were isolated from leaves of 8-d-old barley (Hordeum vulgare L. cv Gerbel) seedlings. Transport of ascorbate and dehydroascorbate into protoplasts and vacuoles was investigated. Contents of ascorbic acid, glutathione, and [alpha]-tocopherol and ascorbate peroxidase activity and glutathione reductase activity were analyzed in protoplasts, vacuoles, and chloroplasts. Uptake of ascorbate and dehydroascorbate by protoplasts showed saturation kinetics (Km = 90 [mu]M reduced ascorbic acid, 20 [mu]M dyhydroascorbic acid). Effects of various membrane transport inhibitors suggested that transport was carrier mediated and driven by a proton electrochemical gradient. Translocation of ascorbate and dehydroascorbate into vacuoles did not show saturation kinetics. Neither was it influenced by effectors or by ATP but only by Mg2+, suggesting that translocation did not occur by carrier. Ascorbic acid was predominantly localized in the cytosol. Contents in the chloroplasts and vacuoles were low. The results are consistent with the view that ascorbate is synthesized in the cytosol and released to chloroplasts, apoplast, and vacuole following a concentration gradient. Translocation from the apoplast into the cytosol is against a steep gradient and appears to control the concentration of ascorbic acid in the apoplast. In its function as an antioxidant, ascorbate in the apoplast may be oxidized to dehydroascorbate, which can be efficiently transported back into the cytosol for regeneration to ascorbate. PMID:12232318

  20. Characterisation of extra virgin olive oils from Galician autochthonous varieties and their co-crushings with Arbequina and Picual cv.

    PubMed

    Reboredo-Rodríguez, P; González-Barreiro, C; Cancho-Grande, B; Fregapane, G; Salvador, M D; Simal-Gándara, J

    2015-06-01

    The current trend of the olive oil market is the production of high quality extra from traditional minor olive varieties with peculiar and differentiated characteristics (especially with respect to the aromatic and phenolic composition). In this way, the interest of Galician oil producers (NW Spain) in recovering old autochthonous Local olive fruits has increased substantially in recent years. In order to investigate the potential of the Local olives by either producing high quality monovarietal oils or mixing with the most widespread olives in Galicia (Arbequina and Picual cv.), quality indices, and fatty acid composition as well as volatile and phenolic profiles were determined and compared. All EVOOs studied in this work can be considered as "extra virgin olive oil" due to quality indices fell within the ranges established in legislation. Picual and Local olive oils as well as those resulting from their co-crushing reach values which are required by EU legislation to add the specific health claim on the oil label. Co-crushing Picual:Local (80:20) provided a significant enhancement of grass and apple nuances and a decrease of banana notes with respect to Picual oils. The co-crushing process improved sensory and health properties of Picual extra virgin olive oils. The effect of co-crushing on phenolics, ester volatiles and banana nuances cannot be easily modulated, contrary to quality indices and fatty acid composition, both changing linearly in strict correlation with the fruit mass ratio. PMID:25624261

  1. Response of different maturity stages of sapota (Manilkara achras Mill.) cv. Kallipatti to in-package ethylene absorbent.

    PubMed

    Bhutia, Wangdup; Pal, R K; Sen, Sangita; Jha, S K

    2011-12-01

    Sapota fruits are highly perishable due to their climacteric nature. The rapid softening of fruits is primarily due to high activity of many oxidative enzymes and liberation of ethylene. Harvest maturity plays a crucial role in deciding the marketability of climacteric fruits in general. Attempt has been made to evaluate the response of ethylene absorbent on variable maturity groups of harvested Sapota cv. Kallipatti with the objective to delay the ripening during transit and extend its marketability during storage at ambient condition (27-32 °C & 65-75% R.H.). Harvested fruits having three different degree of ripeness (as maturity indices viz. mature, half-ripe and ripe) were packed with or without ethylene absorbent sachets (Bioconservación, France) in 10 kg CFB boxes and transported from Dahnu to Delhi covering a distant of approximately 2500 KM by truck on road along with conventional packaging as control. The fruits were evaluated immediately on arrival at Delhi and subsequently during storage for various physical, physiological, biochemical and decay parameters. Mature fruits with ethylene absorbent exhibited maximum delay in ripening, low ethylene liberation, weight loss and high fruit firmness. The response of ethylene absorbent to extend the marketability of ripe fruit was not significant. PMID:23572819

  2. Intake and Performance of Yearling Steers Grazing Guineagrass (Panicum maximum cv. Tanzânia) Pasture Supplemented with Different Energy Sources

    PubMed Central

    Santana, M. C. A.; Euclides, V. B. P.; Mancio, A. B.; Medeiros, S. R.; Costa, J. A. R.; Oliveira, R. L.

    2013-01-01

    The aim of this study was to evaluate the efficiency of supplements containing different energy sources in relation to mineral supplementation of steers grazing guineagrass (Panicum maximum cv Tanzânia) pasture, during the dry season. The experimental design was a randomized block with three treatments and four replications. The treatments consisted of a mineral supplementation and two other supplements, one based on corn seed and the other based on soybean hulls, and provided at 0.8% of body weight. Forty-eight, 12 month-old crossbred steers with an average initial body weight of 267 kg, were assigned to twelve paddocks (1,125 ha) of guineagrass. The animals that were fed with soybean hulls and corn seed presented a greater average daily gain (0.982 and 0.937) when compared with the mineral supplementation. Soybean hulls can be used as a satisfactory food source, replacing corn as an energy source in the supplementation of beef cattle without compromising animal performance. PMID:25049797

  3. 5S program to reduce change-over time on forming department (case study on CV Piranti Works temanggung)

    NASA Astrophysics Data System (ADS)

    Rosiana Dewi, Septika; Setiawan, Budi; P, Susatyo Nugroho W.

    2013-06-01

    Productivity is one aspect that determines the success of a company in the competitive world of business. There are seven main types of activities that do not have value-added in manufacturing processes such as overproduction, waiting time, transportation, excess inventory, unnecessary motion and defects. The whole activity is a waste (waste) that can cause harm to the Company. Therefore, in production activities is important to pay attention so that the objectives of production productivity can be achieved. Problems experienced by CV Piranti Works is a production target is not achieved resulting in a lost sale raises the cost of which can cause harm to the Company. From the analysis conducted major known cause of the problem is the length of time required for changeover. This is supported by the high non-value added activity in the changeover activities. Lean Manufacturing is an approach to make system more efficient by reducing waste. This study refers to the book compiled by Takashi Osada (2004) and several other references. In this research used method 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) for the of forming departement. The purpose of this research is to design a work environment using the 5S method (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) and make arrangement of equipment and working tool cabinet design with TRIZ methods. From these results, is expected to eliminate or reduce of non-value added activity and improved the changeover time so as to meet production targets completion of the company.

  4. Characterization of the adaptive response of grapevine (cv. Tempranillo) to UV-B radiation under water deficit conditions.

    PubMed

    Martínez-Lüscher, J; Morales, F; Delrot, S; Sánchez-Díaz, M; Gomès, E; Aguirreolea, J; Pascual, I

    2015-03-01

    This work aims to characterize the physiological response of grapevine (Vitis vinifera L.) cv. Tempranillo to UV-B radiation under water deficit conditions. Grapevine fruit-bearing cuttings were exposed to three levels of supplemental biologically effective UV-B radiation (0, 5.98 and 9.66kJm(-2)day(-1)) and two water regimes (well watered and water deficit), in a factorial design, from fruit-set to maturity under glasshouse-controlled conditions. UV-B induced a transient decrease in net photosynthesis (Anet), actual and maximum potential efficiency of photosystem II, particularly on well watered plants. Methanol extractable UV-B absorbing compounds (MEUVAC) concentration and superoxide dismutase activity increased with UV-B. Water deficit effected decrease in Anet and stomatal conductance, and did not change non-photochemical quenching and the de-epoxidation state of xanthophylls, dark respiration and photorespiration being alternative ways to dissipate the excess of energy. Little interactive effects between UV-B and drought were detected on photosynthesis performance, where the impact of UV-B was overshadowed by the effects of water deficit. Grape berry ripening was strongly delayed when UV-B and water deficit were applied in combination. In summary, deficit irrigation did not modify the adaptive response of grapevine to UV-B, through the accumulation of MEUVAC. However, combined treatments caused additive effects on berry ripening. PMID:25617319

  5. Effects of elicitors on the production of resveratrol and viniferins in cell cultures of Vitis vinifera L. cv Italia.

    PubMed

    Santamaria, Anna Rita; Mulinacci, Nadia; Valletta, Alessio; Innocenti, Marzia; Pasqua, Gabriella

    2011-09-14

    Methyl jasmonate, jasmonic acid and chitosan were tested as elicitors on cell suspension cultures obtained from Vitis vinifera cv Italia to investigate their effect on stilbene production. Stilbene accumulation in the callus, grown under nonelicited conditions, was also investigated. Calli and cell suspensions were obtained in a B5 culture medium supplemented with 0.2 mg L(-1) NAA and 1 mg L(-1) KIN. Stilbene determination was achieved by HPLC/DAD/MS. Whereas callus biosynthesized only piceid, cell suspensions elicited with jasmonates produced several stilbenes, mainly viniferins. In suspended cells, methyl jasmonate and jasmonic acid were the most effective in stimulating stilbene biosynthesis, whereas chitosan was less effective; in fact, the amount of stilbenes obtained with this elicitor was not significantly different from that obtained for the control cells. The maximum production of total stilbenes was at day 20 of culture with 0.970 and 1.023 mg g(-1) DW for MeJA and JA, respectively. PMID:21751812

  6. James Webb Space Telescope (JWST) Integrated Science Instruments Module (ISIM) Cryo-Vacuum (CV) Test at GSFC

    NASA Technical Reports Server (NTRS)

    Yew, Calinda M.

    2014-01-01

    JWST ISIM has entered into its system-level testing program at NASA Goddard Space Flight Center (GSFC). In December 2013, ISIM successfully completed the first in a series of three cryo-vacuum tests, which included two flight science instruments. Since then, there have been full-fledged efforts towards the CV2 test scheduled to finish at the end of 2014. The complexity of the mission has generated challenging requirements that demand highly reliable system performance and capabilities from the Space Environment Simulator (SES) vacuum chamber. In order to satisfy the program requirements, GSFC had to develop unique structural and thermal hardware to test ISIM. Most noteworthy is a helium shroud structure and cooling system built in order to achieve operational temperatures below 20K (-253C). This paper: (1) provides an overview of the integrated mechanical and thermal facility systems required to achieve the objectives of JWST ISIM testing, (2) communicates the performance and challenges of the SES during the first ISIM test, and (3) summarizes the action plan to improve the system prior to the next test.

  7. Intake and Performance of Yearling Steers Grazing Guineagrass (Panicum maximum cv. Tanzânia) Pasture Supplemented with Different Energy Sources.

    PubMed

    Santana, M C A; Euclides, V B P; Mancio, A B; Medeiros, S R; Costa, J A R; Oliveira, R L

    2013-03-01

    The aim of this study was to evaluate the efficiency of supplements containing different energy sources in relation to mineral supplementation of steers grazing guineagrass (Panicum maximum cv Tanzânia) pasture, during the dry season. The experimental design was a randomized block with three treatments and four replications. The treatments consisted of a mineral supplementation and two other supplements, one based on corn seed and the other based on soybean hulls, and provided at 0.8% of body weight. Forty-eight, 12 month-old crossbred steers with an average initial body weight of 267 kg, were assigned to twelve paddocks (1,125 ha) of guineagrass. The animals that were fed with soybean hulls and corn seed presented a greater average daily gain (0.982 and 0.937) when compared with the mineral supplementation. Soybean hulls can be used as a satisfactory food source, replacing corn as an energy source in the supplementation of beef cattle without compromising animal performance. PMID:25049797

  8. Dehydropyrrolizidine Alkaloids, Including Monoesters with an Unusual Esterifying Acid, from Cultivated Crotalaria juncea (Sunn Hemp cv.’Tropic Sun’)

    PubMed Central

    Colegate, Steven M.; Gardner, Dale R.; Joy, Robert J.; Betz, Joseph M.; Panter, Kip E.

    2012-01-01

    Cultivation of Crotalaria juncea L. (Sunn Hemp cv. ‘Tropic Sun’) is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves and seeds of ‘Tropic Sun’ were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid content of the roots, stems, leaves of immature plants was estimated to be 0.05, 0.12 and 0.01% w/w respectively while seeds were estimated to contain 0.15% w/w. PMID:22429238

  9. Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening

    PubMed Central

    2011-01-01

    Background Cluster thinning is an agronomic practice in which a proportion of berry clusters are removed from the vine to increase the source/sink ratio and improve the quality of the remaining berries. Until now no transcriptomic data have been reported describing the mechanisms that underlie the agronomic and biochemical effects of thinning. Results We profiled the transcriptome of Vitis vinifera cv. Sangiovese berries before and after thinning at veraison using a genome-wide microarray representing all grapevine genes listed in the latest V1 gene prediction. Thinning increased the source/sink ratio from 0.6 to 1.2 m2 leaf area per kg of berries and boosted the sugar and anthocyanin content at harvest. Extensive transcriptome remodeling was observed in thinned vines 2 weeks after thinning and at ripening. This included the enhanced modulation of genes that are normally regulated during berry development and the induction of a large set of genes that are not usually expressed. Conclusion Cluster thinning has a profound effect on several important cellular processes and metabolic pathways including carbohydrate metabolism and the synthesis and transport of secondary products. The integrated agronomic, biochemical and transcriptomic data revealed that the positive impact of cluster thinning on final berry composition reflects a much more complex outcome than simply enhancing the normal ripening process. PMID:22192855

  10. Effect of salicylhydroxamic acid on endosperm strength and embryo growth of Lactuca sativa L. cv Waldmann's Green seeds

    NASA Technical Reports Server (NTRS)

    Brooks, C. A.; Mitchell, C. A.

    1988-01-01

    Salicylhydroxamic acid (SHAM) stimulated germination of photosensitive lettuce (Lactuca sativa L. cv Waldmann's Green) seeds in darkness. To determine whether SHAM acts on the embryo or the endosperm, we investigated separately effects of SHAM on growth potential of isolated embryos as well as on endosperm strength. Embryo growth potential was quantified by incubating decoated embryos in various concentrations of osmoticum and measuring subsequent radicle elongation. Growth potential of embryos isolated from seeds pretreated with 4 millimolar SHAM was equal to that of untreated controls. Rupture strength of endosperm tissue excised from seeds pretreated with SHAM was 33% less than that of controls in the micropylar region. To determine if the embryo must be in contact with the endosperm of SHAM to weaken the endosperm, some endosperms were incubated with SHAM only after dissection from seeds. Rupture strength of SHAM-treated, isolated endosperms in the micropylar region was 25% less than that of untreated controls. There was no difference in rupture strength in the cotyledonary region of endosperm isolated from seeds treated with SHAM in buffer or buffer alone. SHAM therefore stimulates germination not by enhancing embryo growth potential, but by weakening the micropylar region of the endosperm enclosing the embryo.

  11. Profiles of phenolic compounds and purine alkaloids during the development of seeds of Theobroma cacao cv. Trinitario.

    PubMed

    Pereira-Caro, Gema; Borges, Gina; Nagai, Chifumi; Jackson, Mel C; Yokota, Takao; Crozier, Alan; Ashihara, Hiroshi

    2013-01-16

    Changes occurring in phenolic compounds and purine alkaloids, during the growth of seeds of cacao (Theobroma cacao) cv. Trinitario, were investigated using HPLC-MS/MS. Extracts of seeds with a fresh weight of 125, 700, 1550, and 2050 mg (stages 1-4, respectively) were analyzed. The phenolic compounds present in highest concentrations in developing and mature seeds (stages 3 and 4) were flavonols and flavan-3-ols. Flavan-3-ols existed as monomers of epicatechin and catechin and as procyanidins. Type B procyanidins were major components and varied from dimers to pentadecamer. Two anthocyanins, cyanidin-3-O-arabinoside and cyanidin-3-O-galactoside, along with the N-phenylpropernoyl-l-amino acids, N-caffeoyl-l-aspartate, N-coumaroyl-l-aspartate, N-coumaroyl-3-hydroxytyrosine (clovamide), and N-coumaroyltyrosine (deoxyclovamide), and the purine alkaloids theobromine and caffeine, were present in stage 3 and 4 seeds. Other purine alkaloids, such as theophylline and additional methylxanthines, did not occur in detectable quantities. Flavan-3-ols were the only components to accumulate in detectable quantities in young seeds at developmental stages 1 and 2. PMID:23215441

  12. Spectral variability of /s/ in sV and sCV sequences produced by adults and children

    NASA Astrophysics Data System (ADS)

    Munson, Benjamin

    2002-05-01

    Previous research has demonstrated that both children and adults produce /s/ with greater spectral variability in /sp/ sequences than /st/ sequences, when these sequences are embedded in the medial position of CVCCVC nonwords [B. Munson, J. Acoust. Soc. Am. 110, 1203-1206 (2001)]. The current study examined whether this result could be replicated when /s/ is embedded in syllable-onset clusters, with a variety of following consonants and vowels. Adults and children aged 3-7 were recorded producing multiple tokens of sV and sCV nonwords, where the vowel was either /i/, /opena/, or /u/, and the consonant was either /p/, /t/, /w/, or /l/. For each token, the spectral mean of non-overlapping 10-ms windows of frication noise was calculated. Nonlinear regressions of the form y=aebx were used to predict the spectral mean of each portion of frication noise from its position in the fricative. The resulting measure of model fit, R2, was used as an index of within-speaker variability. For each participant, separate R2 values were calculated for /s/ in each of the 15 phonetic contexts. Analyses will address the influence of age, consonant context, and vowel context on spectral variability.

  13. Some observations of the effects of applied magnetic fields on growth of Avena coleoptiles. [Avena sativa cv. Garry

    SciTech Connect

    Brown, D.S.; Gretz, M.; Brown, R.M. Jr.

    1986-04-01

    Applied magnetic fields (5000-18,000 Gauss) disrupt the pattern of cellulose microfibril deposition in cells of Avena sativa cv. Garry coleoptiles. Freeze fracture reveals the microfibrils of the innermost wall layers to be randomly oriented, rather than parallel and transverse to the elongation axis as seen in control plants. Whole plants exposed to the magnetic field at any field intensity tested (5000-18,000 Gauss) displayed inhibited elongation growth. After two hours in a 5000 G field, plants show some recovery of elongation growth while maintained in the field, with elongation rates of 0.2 + 0.06 mm/hr as compared to control plants with 0.9 + 0.12 mm/hr elongation. Avena seeds which were germinated directly between pole pieces of a 5000 Gauss magnet produced slow-growing seedlings whose roots and coleoptiles grew toward opposite magnetic poles. Audus (1960) coined the phrase magnetotropism to describe a similar phenomenon in pollen tubes growing in magnetic fields.

  14. Cotton gene expression profiles in resistant Gossypium hirsutum cv. Zhongzhimian KV1 responding to Verticillium dahliae strain V991 infection.

    PubMed

    Zhang, Wen-Wei; Jian, Gui-Liang; Jiang, Teng-Fei; Wang, Sheng-Zheng; Qi, Fang-Jun; Xu, Shi-Chang

    2012-10-01

    Verticillium wilt of cotton (Gossypium hirsutum) is a widespread and destructive disease that is caused by the soil-borne fungus pathogen Verticillium dahliae (V. dahliae). To study the molecular mechanism in wilt tolerance, suppression subtractive hybridization (SSH) and dot blot techniques were used to identify the specifically expressed genes in a superior wilt-resistant cotton cultivar (G. hirsutum cv. Zhongzhimian KV1) after inoculation with pathogen. cDNAs from the root tissues of Zhongzhimian KV1 inoculated with V. dahliae strain V991 or water mock were used to construct the libraries that contain 4800 clones. Based on the results from dot blot analysis, 147 clones were clearly induced by V. dahliae and selected from the SSH libraries for sequencing. A total of 92 up-regulated and 7 down-regulated non-redundant expressed sequences tags (ESTs) were identified as disease responsive genes and classified into 9 functional groups. Two important clues regarding wilt-resistant G. hirsutum were obtained from this study. One was Bet v 1 family; the other was UbI gene family that may play an important role in the defense reaction against Verticillium wilt. The result from real-time quantitative reverse transcription polymerase chain reaction showed that these genes were activated quickly and transiently after inoculation with V. dahliae. PMID:22733494

  15. Molecular cloning and expression of a gene encoding alcohol acyltransferase (MdAAT2) from apple (cv. Golden Delicious).

    PubMed

    Li, Dapeng; Xu, Yunfeng; Xu, Gangming; Gu, Lingkun; Li, Dequan; Shu, Huairui

    2006-04-01

    Volatile esters are major aroma components of apple, and an alcohol acyltransferase (AAT) catalyzes the final step in ester biosynthesis. The gene MdAAT2, which encodes a predicted 51.2 kDa protein containing features of other acyl transferases, was isolated from Malus domestica Borkh. (cv. Golden Delicious). In contrast to other apple varieties, the MdAAT2 gene of Golden Delicious is exclusively expressed in the fruit. The MdAAT2 protein is about 47.9 kDa and mainly localized in the fruit peel, as indicated by immunoblot and immunolocalization analysis. Northern blot and immunoblot analysis showed that the transcription and translation of MdAAT2 have a positive correlation with apple AAT enzyme activity and ester production, except in the later ripening stage, suggesting that MdAAT2 is involved in the regulation of ester biosysthesis and that a post-translation modification may be involved in regulation of AAT enzyme activity. Tissue disk assays of fruit peel revealed that using extraneous alcohols can recover the corresponding ester formation. Transcription and translation of MdAAT2 were both depressed by 1-methylcyclopropene (1-MCP) treatment and subsequent ester production was also prevented. These results suggest that: (1) ester production is mainly regulated by MdAAT2; (2) ethylene is also involved in this regulatory progress and (3) ester compounds rely principally on the availability of substrates. PMID:16524607

  16. Nitrogen balance for wheat canopies (Triticum aestivum cv. Veery 10) grown under elevated and ambient CO2 concentrations

    NASA Technical Reports Server (NTRS)

    Smart, D. R.; Ritchie, K.; Bloom, A. J.; Bugbee, B. B.

    1998-01-01

    We examined the hypothesis that elevated CO2 concentration would increase NO3- absorption and assimilation using intact wheat canopies (Triticum aestivum cv. Veery 10). Nitrate consumption, the sum of plant absorption and nitrogen loss, was continuously monitored for 23 d following germination under two CO2 concentrations (360 and 1000 micromol mol-1 CO2) and two root zone NO3- concentrations (100 and 1000 mmol m3 NO3-). The plants were grown at high density (1780 m-2) in a 28 m3 controlled environment chamber using solution culture techniques. Wheat responded to 1000 micromol mol-1 CO2 by increasing carbon allocation to root biomass production. Elevated CO2 also increased root zone NO3- consumption, but most of this increase did not result in higher biomass nitrogen. Rather, nitrogen loss accounted for the greatest part of the difference in NO3- consumption between the elevated and ambient [CO2] treatments. The total amount of NO3(-)-N absorbed by roots or the amount of NO3(-)-N assimilated per unit area did not significantly differ between elevated and ambient [CO2] treatments. Instead, specific leaf organic nitrogen content declined, and NO3- accumulated in canopies growing under 1000 micromol mol-1 CO2. Our results indicated that 1000 micromol mol-1 CO2 diminished NO3- assimilation. If NO3- assimilation were impaired by high [CO2], then this offers an explanation for why organic nitrogen contents are often observed to decline in elevated [CO2] environments.

  17. Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans

    PubMed Central

    Lee, Jong-Kook; Gopal, Ramamourthy; Seo, Chang Ho; Cheong, Hyeonsook; Park, Yoonkyung

    2012-01-01

    In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 ?g/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys3 and Cys9 or Cys10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents. PMID:22605963

  18. Herbicide Treatments Alter Carotenoid Profiles for 14C Tracer Production from Tomato (Solanum lycopersicum cv. VFNT cherry) Cell Cultures

    PubMed Central

    Engelmann, Nancy J.; Rogers, Randy B.; Lila, Mary Ann; Erdman, John W.

    2009-01-01

    Progress in learning about underlying carotenoid bioactivity mechanisms has been limited due to lack of commercially available radiolabeled lycopene (LYC), phytoene (PE), and phytofluene (PF). Tomato (Solanum lycopersicum cv. VFNT cherry) cell cultures have been treated to produce [14C]-PE and PF but with relatively low yields. To increase carotenoid production, two bleaching herbicides were administered during the culture incubation, 2-(4-chlorophenyl-thio) triethylamine and norflurazon separately or in combination to produce varying ratios of PE, PF, and LYC. Treatment with both herbicides resulted in optimal production of all three carotenoids. Subsequently, cultures were incubated in [14C] sucrose-containing media to produce labeled LYC, PE, and PF. Adding [14C] sucrose on day 1 of the 14-d culture incubation cycle to norflurazon-treated cultures led to a small increase in labeling efficiency compared to adding it on day 7. Improved culture conditions efficiently provided sufficient 14C-carotenoids for future cell culture and animal metabolic tracking studies. PMID:19489618

  19. Heavy metal accumulation and tolerance of energy grass (Elymus elongatus subsp. ponticus cv. Szarvasi-1) grown in hydroponic culture.

    PubMed

    Sipos, Gyula; Solti, Adám; Czech, Viktória; Vashegyi, Ildikó; Tóth, Brigitta; Cseh, Edit; Fodor, Ferenc

    2013-07-01

    Phytoremediation is a plant based, cost effective technology to detoxify or stabilise contaminated soils. Fast growing, high biomass, perennial plants may be used not only in phytoremediation but also in energy production. Szarvasi-1 energy grass (Elymus elongatus subsp. ponticus cv. Szarvasi-1), a good candidate for this combined application, was grown in nutrient solution in order to assess its Cd, Cu, Ni, Pb and Zn accumulation and tolerance. Its shoot metal accumulation showed the order Pb < Ni < Cu ? Cd < Zn. In parallel with this, Pb and Ni had no or very little influence on the growth, dry matter content, chlorophyll concentration and transpiration of the plants. Cu and Cd treatment resulted in significant decreases in all these parameters that can be attributed to Fe plaque formation in the roots suggested by markedly increased Fe and Cu accumulation. This came together with decreased shoot and root Mn concentrations in both treatments while shoot Cu and Zn concentrations decreased under Cd and Cu exposure, respectively. Zn treatment had no effect or even slightly stimulated the plants. This may be due to a slight stimulation of Fe translocation and a very efficient detoxification mechanism. Based on the average 300 mg kg?¹ (dry mass) Zn concentration which is 0.03% of the shoot dry mass the variety is suggested to be classified as Zn accumulator. PMID:23669138

  20. Separating para and ortho water.

    PubMed

    Horke, Daniel A; Chang, Yuan-Pin; D?ugo??cki, Karol; Küpper, Jochen

    2014-10-27

    Water exists as two nuclear-spin isomers, para and ortho, determined by the overall spin of its two hydrogen nuclei. For isolated water molecules, the conversion between these isomers is forbidden and they act as different molecular species. Yet, these species are not readily separated, and no pure para sample has been produced. Accordingly, little is known about their specific physical and chemical properties, conversion mechanisms, or interactions. The production of isolated samples of both spin isomers is demonstrated in pure beams of para and ortho water in their respective absolute ground state. These single-quantum-state samples are ideal targets for unraveling spin-conversion mechanisms, for precision spectroscopy and fundamental symmetry-breaking studies, and for spin-enhanced applications, for example laboratory astrophysics and astrochemistry or hypersensitized NMR experiments. PMID:25196938

  1. The CC-V Flap: A Novel Technique for Augmenting a C-V Nipple Reconstruction Using a Free Dermal Graft

    PubMed Central

    Elizabeth Clark, Sarah; Turton, EPL

    2014-01-01

    BACKGROUND We present a novel method for augmenting the standard C-V flap used for nipple reconstruction with a free dermal graft which aims to improve the appearance of the nipple reconstruction, decrease loss of projection and improve patient satisfaction overall. METHODS The surgical technique for performing a free dermal graft augmentation of a CV flap is described. All patients who underwent this technique between February 2009 and January 2012 at our unit were contacted by telephone, questioned about any complications and asked to rate their satisfaction with the technique. RESULTS In a series of 18 nipple reconstructions, there were no immediate post-operative complications and long term follow up shows that that this technique achieves high patient satisfaction scores. CONCLUSIONS The CC-V flap is a safe technique which scores highly with patients for cosmetic appearance after long term follow up. PMID:25489517

  2. The use of Lactobacillus pentosus 1MO to shorten the debittering process time of black table olives (Cv. Itrana and Leccino): a pilot-scale application.

    PubMed

    Servili, Maurizio; Settanni, Luca; Veneziani, Gianluca; Esposto, Sonia; Massitti, Ombretta; Taticchi, Agnese; Urbani, Stefania; Montedoro, Gian Francesco; Corsetti, Aldo

    2006-05-31

    Fifty lactobacilli isolated from black table olive brines were evaluated for their salt tolerance, resistance to oleuropein and verbascoside, and ability to grow in modified filter-sterilized brines. A strain of Lactobacillus pentosus was selected and used as a starter to ferment, in pilot plant, black olives (Itrana and Leccino cv.) in brines modified for pH, carbohydrate, and growth factor concentrations, at 28 degrees C. The temperature-controlled fermentation of Leccino cv. olives resulted in obtaining ready-to-eat, high-quality table olives in a reduced-time process. HPLC analysis of phenolic compounds from fermented olives showed a decrease of oleuropein, a glucoside secoiridoid responsible for the bitter taste of olive drupes, and an increase of the hydroxytyrosol concentration. The selected strain of L. pentosus (1MO) allowed the reduction of the debittering phase period to 8 days. PMID:16719508

  3. SEGURIDAD ENERGETICASEGURIDAD ENERGETICA UN DESAFIO PARA CHILEUN DESAFIO PARA CHILE

    E-print Network

    Catholic University of Chile (Universidad Católica de Chile)

    Universidad Católica de Chile Departamento de Ingeniería Eléctrica Hugh Rudnick 24 Noviembre 2006 #12;2 Tabla Producto Geográfico Bruto versus Consumo Energético (Fuente: CNE y Banco Central) Desarrollo económico y con petróleo a EEUU en relación a Israel el año 1970 ·Venezuela utilizando energía para influir en

  4. Petrography, stable isotope compositions, microRaman spectroscopy, and presolar components of Roberts Massif 04133: A reduced CV3 carbonaceous chondrite

    NASA Astrophysics Data System (ADS)

    Davidson, Jemma; Schrader, Devin L.; Alexander, Conel M. O'd.; Lauretta, Dante S.; Busemann, Henner; Franchi, Ian A.; Greenwood, Richard C.; Connolly, Harold C.; Domanik, Kenneth J.; Verchovsky, Alexander

    2014-12-01

    Here, we report the mineralogy, petrography, C-N-O-stable isotope compositions, degree of disorder of organic matter, and abundances of presolar components of the chondrite Roberts Massif (RBT) 04133 using a coordinated, multitechnique approach. The results of this study are inconsistent with its initial classification as a Renazzo-like carbonaceous chondrite, and strongly support RBT 04133 being a brecciated, reduced petrologic type >3.3 Vigarano-like carbonaceous (CV) chondrite. RBT 04133 shows no evidence for aqueous alteration. However, it is mildly thermally altered (up to approximately 440 °C); which is apparent in its whole-rock C and N isotopic compositions, the degree of disorder of C in insoluble organic matter, low presolar grain abundances, minor element compositions of Fe,Ni metal, chromite compositions and morphologies, and the presence of unequilibrated silicates. Sulfides within type I chondrules from RBT 04133 appear to be pre-accretionary (i.e., did not form via aqueous alteration), providing further evidence that some sulfide minerals formed prior to accretion of the CV chondrite parent body. The thin section studied contains two reduced CV3 lithologies, one of which appears to be more thermally metamorphosed, indicating that RBT 04133, like several other CV chondrites, is a breccia and thus experienced impact processing. Linear foliation of chondrules was not observed implying that RBT 04133 did not experience high velocity impacts that could lead to extensive thermal metamorphism. Presolar silicates are still present in RBT 04133, although presolar SiC grain abundances are very low, indicating that the progressive destruction or modification of presolar SiC grains begins before presolar silicate grains are completely unidentifiable.

  5. Managing rhodes grass (Chloris gayana) cv. Callide to improve diet quality. 1. Effects of age of regrowth, strip grazing and mulching

    Microsoft Academic Search

    W. K. EHRLICH; R. T. COWAN; K. F. LOWE

    Two experiments assessed grazing management strategies for irrigated and nitrogen- fertilised rhodes grass (Chloris gayana) cv. Callide pas- ture. In Experiment 1, the 4 treatments were: (i) open grazing of the entire paddock on a 2-week cycle (Open); (ii) grazing on a 2-week, daily strip-grazing rotation (2); (iii) a 4-week, daily strip-grazing rotation (4); and (iv) a 6-week, daily strip-grazing

  6. Analysis of the Expression of Anthocyanin Pathway Genes in Developing Vitis vinifera 1. cv Shiraz Grape Berries and the - Implications for Pathway Regdation

    Microsoft Academic Search

    Paul K. BOSS; Christopher Davies; Simon P. Robinson

    Anthocyanin synthesis in Vitis vinifera L. cv Shiraz grape berries began 1 O weeks postflowering and continued throughout berry ripening. Expression of seven genes of the anthocyanin biosynthetic pathway (phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone-3-hydroxylase (F3Hl, dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygen- ase (LDOX), and UDP glucose-flavonoid 3-o-glucosyl transferase (UFCT)) was determined. In flowers and grape

  7. Long-Term Fungal Inhibitory Activity of Water-Soluble Extracts of Phaseolus vulgaris cv. Pinto and Sourdough Lactic Acid Bacteria during Bread Storage

    Microsoft Academic Search

    Rossana Coda; Carlo G. Rizzello; Franco Nigro; Maria De Angelis; Philip Arnault; Marco Gobbetti

    2008-01-01

    The antifungal activity of proteinaceous compounds from different food matrices was investigated. In initial experiments, water-soluble extracts of wheat sourdoughs, cheeses, and vegetables were screened by agar diffusion assays with Penicillium roqueforti DPPMAF1 as the indicator fungus. Water-soluble extracts of sourdough fermented with Lactobacillus brevis AM7 and Phaseolus vulgaris cv. Pinto were selected for further study. The crude water-soluble extracts

  8. Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester induces systemic resistance in tomato ( Lycopersicon esculentum. Mill cv. Vollendung) to Cucumber mosaic virus

    Microsoft Academic Search

    Ghandi H. Anfoka

    2000-01-01

    The potential of benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) to trigger systemic acquired resistance (SAR) in tomato (Lycopersicon esculentum. Mill cv. Vollendung) plants against a yellow strain of Cucumber mosaic virus (CMV-Y) was investigated. Application of BTH, as a drench, 7 days before inoculation with CMV-Y, protected plants against the necrosis caused by CMV-Y. The resistance was evident as a decreased

  9. Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR)

    Microsoft Academic Search

    B. Chaudhary; S. Kumar; K. V. S. K. Prasad; G. S. Oinam; P. K. Burma; D. Pental

    2003-01-01

    In Agrobacterium-mediated genetic transformation of cotton ( Gossypium hirsutum L. cv. Coker 310FR) the frequency at which somatic embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. We used Agrobacterium strain GV3101 containing the binary vector pGSFR with the nos-nptII gene for in vitro selection and the 35S  gus-int fragment as a reporter

  10. Aflatoxin — induced alteration in the levels of membrane chemicals of subcellular organelles isolated from excised, incubated Glycine max, cv. ‘Essex’ roots

    Microsoft Academic Search

    J. M. Danley; S. Staggers; S. Walker; A. Varnert; G. C. Llewellyn; W. V. Dashek

    1981-01-01

    Isolates of aflatoxin-producing strains of Aspergillus grow on autoclaved and field-grown (lesser extent) Glycine max beans. Both mixed and aflatoxin B1 inhibit G. max, cv. ‘Essex’ bean germination and elongation of either attached or excised cultured roots. Because B1 impairs the latter roots' ability to intracellularize [14C]-leucine, it may alter plasmalemma structure and\\/or function. To determine whether incubation of excised

  11. Mineralogy and petrography of amoeboid olivine aggregates from the reduced CV3 chondrites Efremovka, Leoville and Vigarano: Products of nebular condensation, accretion and annealing

    NASA Astrophysics Data System (ADS)

    Komatsu, Mutsumi; Krot, Alexander N.; Petaev, Mikhail I.; Ulyanov, Alexander A.; Keil, Klaus; Miyamoto, Masamichi

    2001-05-01

    Amoeboid olivine aggregates (AOAs) from the reduced CV chondrites Efremovka, Leoville and Vigarano are irregularly-shaped objects, up to 5 mm in size, composed of forsteritic olivine (Fa<10) and a refractory, Ca,Al-rich component. The AOAs are depleted in moderately volatile elements (Mn, Cr, Na, K), Fe,Ni-metal and sulfides and contain no low-Ca pyroxene. The refractory component consists of fine-grained Ca,Al-rich inclusions (CAIs) composed of Al-diopside, anorthite (An100), and magnesium-rich spinel (~1 wt% FeO) or fine-grained intergrowths of these minerals; secondary nepheline and sodalite are very minor. This indicates that AOAs from the reduced CV chondrites are more pristine than those from the oxidized CV chondrites Allende and Mokoia. Although AOAs from the reduced CV chondrites show evidence for high temperature nebular annealing (e.g., forsterite grain boundaries form 120 deg triple junctions) and possibly a minor degree of melting of Al-diopside-anorthite materials, none of the AOAs studied appear to have experienced extensive (>50%) melting. We infer that AOAs are aggregates of high temperature nebular condensates, which formed in CAI-forming regions, and that they were absent from chondrule-forming regions at the time of chondrule formation. The absence of low-Ca pyroxene and depletion in moderately volatile elements (Mn, Cr, Na, K) suggest that AOAs were either removed from CAI-forming regions prior to condensation of these elements and low-Ca pyroxene or gas-solid condensation of low-Ca-pyroxene was kinetically inhibited.

  12. Differential gene expression analysis by cDNA-AFLP between flower buds of Phalaenopsis Hsiang Fei cv. H. F. and its somaclonal variant

    Microsoft Academic Search

    Tsai-Wei Hsu; Wen-Chieh Tsai; Dan-Ping Wang; Sandy Lin; Yu-Yun Hsiao; Wen-Huei Chen; Hong-Hwa Chen

    2008-01-01

    Somaclonal variation occurs during plant tissue culture and introduces changes that can result in the development of desirable traits. Using cDNA-amplified restriction fragment length polymorphism (cDNA-AFLP) analysis, we compared gene expression patterns between flower buds from wild type (donor) plants of Phalaenopsis Hsiang Fei cv. H. F., whose flowers are bronze in color, and from its somaclonal variants, whose flowers

  13. Does nitrogen supply affect the response of wheat (Triticum aestivum cv. Hanno) to the combination of elevated CO2 and O3?

    Microsoft Academic Search

    Joao Cardoso-Vilhena; Jeremy Barnes

    2001-01-01

    Spring wheat (Triticum aestivum cv. Hanno) was grown at ambient (350 mmol mol? 1) or elevated CO2 (700 mmol mol? 1) in charcoaluPurafil1-filtered air (CFA - 5n mol mol ? 1) or ozone (CFA q75 nmol mol? 1 7h d? 1) at three levels of N supply (1.5, 4 and 14 mM NO?3 ), to test the hypothesis that the

  14. Lack of effect of aspirin in primary CV prevention in type 2 diabetic patients with nephropathy: results from 8 years follow-up of NID-2 study.

    PubMed

    Sasso, Ferdinando Carlo; Marfella, Raffaele; Pagano, Antonio; Porta, Giovanni; Signoriello, Giuseppe; Lascar, Nadia; Minutolo, Roberto; Carbonara, Ornella; Persico, Marcello; Piscione, Federico; De Nicola, Luca; Torella, Roberto; Paolisso, Giuseppe

    2015-04-01

    The risk-to-benefit ratio for the use of low dose of aspirin in primary cardiovascular (CV) prevention in patients with diabetes mellitus remains to be clarified. We assessed the effect of aspirin on risk of CV events in type 2 diabetic patients with nephropathy, in order to verify the usefulness of Guidelines in clinical practice. We carried out a prospective multicentric study in 564 patients with type 2 diabetic nephropathy free of CV disease attending outpatient diabetes clinics . A total of 242 patients received antiplatelet treatment with aspirin 100 mg/day (group A), and 322 were not treated with antiplatelet drugs (group B). Primary end point was the occurrence of total major adverse cardio-vascular events (MACE). Secondary end points were the relative occurrence of fatal MACE. The average follow-up was 8 years. Total MACE occurred in 49 patients from group A and in 52 patients from group B. Fatal MACE occurred in 22 patients from group A and in 20 from group B; nonfatal MACE occurred in 27 patients from group A and in 32 patients from group B. Kaplan-Meier analysis did not show a statistically significant difference of cumulative MACE between the two groups. A not statistically significant difference in the incidence of both fatal (p = 0.225) and nonfatal CV events (p = 0.573) between the two groups was observed. These results were confirmed after adjustment for confounders (HR for MACE 1.11, 95 % CI 0.91-1.35). These findings suggest that low dose of aspirin is ineffective in primary prevention for patients with nephropathy. PMID:25109286

  15. Application of silver nanoparticles contained in ethanol as a working fluid in an oscillating heat pipe with a check valve (CLOHP/CV): a thermodynamic behaviour study

    NASA Astrophysics Data System (ADS)

    Bhuwakietkumjohn, N.; Parametthanuwat, T.

    2015-01-01

    This research focused on the thermal behaviour of oscillating heat pipe with a check valve (CLOHP/CV). The CLOHP/CV was made from a copper capillary tube with an internal diameter of 2.03 mm, 40 turns, and 2 check valves. The CLOHP/CV had three sections: the evaporator and adiabatic condenser of a length of 50, 100, and 150 mm. The angles of inclinations were 90°, 80°, 60°, 40°, 20° and 0° at normal operating conditions. The two working fluids were an ethanol and silver nanoparticles concentration of 0.5 wt% contained in the ethanol (NE). The filling ratio was 50 % with respect to the total volume of the tube. The operating temperature (T w ) was 45, 65, 85, 105, 125 and 150 °C. The results of the study showed that the heat flux increased significantly when the operating temperature increased, and the heat flux increased when the L e decreased from 150 to 50 mm. Furthermore, the thermal behaviour of CLOHP/CV showed the highest heat flux of 2,012.63 W/m2 at L e of 50, with an angle of inclination of 90° and operating temperature of 150 °C with NE. The behaviour of 0.5 wt% of silver nanoparticles contained in the ethanol produced a good contact angle. The 0.5 wt% of silver nanoparticles decreased the wet ability, thus increasing the thermal behaviour. The optimum concentration for the addition of silver nanoparticles in the working fluid was 0.5 wt%.

  16. Aging skeletal muscle shows a drastic increase in the small heat shock proteins ?B-crystallin\\/HspB5 and cvHsp\\/HspB7

    Microsoft Academic Search

    Philip Doran; Joan Gannon; Kathleen O’Connell; Kay Ohlendieck

    2007-01-01

    Most heat shock proteins operate as molecular chaperones and play a central role in the maintenance of normal cellular function. In skeletal muscle, members of the ?-crystallin domain-containing family of small heat shock proteins are believed to form a cohort of essential stress proteins. Since ?B-crystallin (?BC\\/HspB5) and the cardiovascular heat shock protein (cvHsp\\/HspB7) are both implicated in the molecular

  17. Zygomatic Smile Detection: The Semi-Supervised Haar Training of a Fast and Frugal System: A Gift to OpenCV Community

    Microsoft Academic Search

    D. D. Hromada; C. Tijus; S. Poitrenaud; J. Nadel

    2010-01-01

    Five different OpenCV-compatible XML haar cascades of zygomatic smile detectors as well as five SMILEsamples from which these detectors were derived had been trained and are presented hereby as a new open source SMILEsmileD package. Samples have been extended in an incremental learning fashion, exploiting previously trained detector in order to add and label new elements of positive example set.

  18. Isolation of disease-tolerant cotton ( Gossypium hirsutum L. cv. SVPR 2) plants by screening somatic embryos with fungal culture filtrate

    Microsoft Academic Search

    M. Ganesan; N. Jayabalan

    2006-01-01

    We report an in vitro selection method that has led to isolation of Fusarium wilt and Alternaria leaf spot disease-tolerant plantlets in cotton (Gossypium hirsutum L. cv. SVPR2). Embryogenic callus was isolated from hypocotyl explants of cotton cultured on 5–50% Fusarium oxysporum culture filtrate-fortified callus induction medium. Somatic embryos tolerant to fungal culture filtrate (FCF) were isolated from this embryogenic

  19. ICP OES and CV AAS in determination of mercury in an unusual fatal case of long-term exposure to elemental mercury in a teenager.

    PubMed

    Lech, Teresa

    2014-04-01

    In this work, a case of deliberate self-poisoning is presented. A 14-year-old girl suddenly died during one of the several hospitalizations. Abdominal computer tomography showed a large number of metallic particles in the large intestine. Analysis of blood and internal organs for mercury and other toxic metals carried out by inductively coupled plasma optical emission spectrometry (ICP OES) revealed high concentrations of mercury in kidneys and liver (64,200 and 2470ng/g, respectively), less in stomach (90ng/g), and none in blood. Using cold vapor-atomic absorption spectrometry (CV AAS), high levels of mercury were confirmed in all examined materials, including blood (87ng/g), and additionally in hair. The results of analysis obtained by two techniques revealed that the exposure to mercury was considerable (some time later, it was stated that the mercury originated from thermometers that had been broken over the course of about 1 year, because of Münchausen syndrome). CV AAS is a more sensitive technique, particularly for blood samples (negative results using ICP OES), and tissue samples - with LOQ: 0.63ng/g of Hg (CV AAS) vis-à-vis 70ng/g of Hg (ICP OES). However, ICP OES may be used as a screening technique for autopsy material in acute poisoning by a heavy metal, even one as volatile as mercury. PMID:24630410

  20. Infection of CV1 cells expressing the polyoma virus middle T antigen or the SV40 agnogene product with simian virus 40 host-range mutants.

    PubMed

    Spence, S L; Tack, L C; Wright, J H; Carswell, S; Pipas, J M

    1990-06-01

    SV40 viruses bearing mutations at the carboxy-terminus of large T antigen exhibit a host-range phenotype: such viruses are able to grow in BSC monkey kidney cells at 37 degrees C, but give at least 10,000-fold lower yields than wild type virus in BSC cells at 32 degrees C or in CV1 monkey kidney cells at either temperature. The block to infection in the nonpermissive cell type occurs after the onset of viral DNA replication. Infectious progeny virions are produced at very low efficiency. Although capsid proteins are synthesized at decreased levels, this does not account for the magnitude of the defect. Presumably some step of virion assembly or maturation is affected in these mutants. We have previously reported that the viral agnogene product, a protein thought to be involved in viral assembly or release, fails to accumulate in CV1 cells infected with host-range mutants. In polyoma virus the middle T antigen plays a role in virion maturation by influencing the phosphorylation of capsid proteins. In this communication we show that host-range mutants fail to undergo productive infection of CV1 cells expressing middle T antigen. These mutants do form plaques on an agnoprotein-expressing cell line. However, the agnoprotein does not seem to act by correcting the mutational block but rather increases the efficiency of plaque formation. PMID:2162817

  1. Hydrothermal origin of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A CAI from the Northwest Africa 2086 CV3 chondrite

    NASA Astrophysics Data System (ADS)

    Fintor, Krisztian; Park, Changkun; Nagy, Szabolcs; Pál-Molnár, Elemér; Krot, Alexander N.

    2014-05-01

    We report an occurrence of hexagonal CaAl2Si2O8 (dmisteinbergite) in a compact type A calcium-aluminum-rich inclusion (CAI) from the CV3 (Vigarano-like) carbonaceous chondrite Northwest Africa 2086. Dmisteinbergite occurs as approximately 10 ?m long and few micrometer-thick lath-shaped crystal aggregates in altered parts of the CAI, and is associated with secondary nepheline, sodalite, Ti-poor Al-diopside, grossular, and Fe-rich spinel. Spinel is the only primary CAI mineral that retained its original O-isotope composition (?17O ~ -24‰); ?17O values of melilite, perovskite, and Al,Ti-diopside range from -3 to -11‰, suggesting postcrystallization isotope exchange. Dmisteinbergite, anorthite, Ti-poor Al-diopside, and ferroan olivine have 16O-poor compositions (?17O ~ -3‰). We infer that dmisteinbergite, together with the other secondary minerals, formed by replacement of melilite as a result of fluid-assisted thermal metamorphism experienced by the CV chondrite parent asteroid. Based on the textural appearance of dmisteinbergite in NWA 2086 and petrographic observations of altered CAIs from the Allende meteorite, we suggest that dmisteinbergite is a common secondary mineral in CAIs from the oxidized Allende-like CV3 chondrites that has been previously misidentified as a secondary anorthite.

  2. Compatibilidad con audfonos para sordos

    E-print Network

    Greenberg, Albert

    Compatibilidad con audífonos para sordos Hearing Aid Compatibility Q&A Q. What does hearing aid the different features of the phone thoroughly and in different locations, using your hearing aid or cochlear implant, to determine if you hear any interfering noise. Consult a sales representative about phone return

  3. Prmio para trs novas aplicaes

    E-print Network

    Instituto de Sistemas e Robotica

    Prémio para três novas aplicações Tecnologia. A "wiride" venceu o "Lisbon Big Apps", concurso de Instituto Superior Técnico que desenvolveu o projeto, citada pelo/dAT, a "wiride" é uma nova forma de andar

  4. Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening

    PubMed Central

    Dautt-Castro, Mitzuko; Ochoa-Leyva, Adrian; Contreras-Vergara, Carmen A.; Pacheco-Sanchez, Magda A.; Casas-Flores, Sergio; Sanchez-Flores, Alejandro; Kuhn, David N.; Islas-Osuna, Maria A.

    2015-01-01

    Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. “Kent” was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ? 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like “cell wall,” “carbohydrate catabolic process” and “starch and sucrose metabolic process” among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening. PMID:25741352

  5. Effects of salicylic acid and putrescine on storability, quality attributes and antioxidant activity of plum cv. 'Santa Rosa'.

    PubMed

    Davarynejad, Gholam Hossein; Zarei, Mehdi; Nasrabadi, Mohamad Ebrahim; Ardakani, Elham

    2015-04-01

    Plum fruit has a short shelf life with a rapid deterioration in quality after harvest. The primary goal of this study is to investigate and compare the effect of putrescine and salicylic acid on quality properties and antioxidant activity of plum during storage. The plum fruits (cv. 'Santa Rosa') were harvested at the mature ripe stage, and dipped in different concentrations of putrescine (1, 2, 3 and 4 mmol/L) and salicylic acid (1, 2, 3 and 4 mmol/L), as well as distilled water (control) for 5 min. The fruits were then packed in boxes with polyethylene covers and stored at 4 °C with 95 % relative humidity for 25 days. A factorial trial based on completely randomized block design with 4 replications was carried out. The weight loss, fruit firmness, total soluble solids, titratable acidity, pH, maturity index, ascorbic acid, total phenolics and antioxidant activity at 0, 5, 10, 15, 20 and 25 days after harvest were recorded. During the storage period, the weight loss, total soluble solids, pH and maturity index increased significantly while the fruit firmness, titratable acidity, ascorbic acid, total phenolics and antioxidant activity decreased significantly (P?

  6. Cloning, expression and immunolocalization pattern of a cinnamyl alcohol dehydrogenase gene from strawberry (Fragaria x ananassa cv. Chandler).

    PubMed

    Blanco-Portales, R; Medina-Escobar, N; López-Ráez, J A; González-Reyes, J A; Villalba, J M; Moyano, E; Caballero, J L; Muñoz-Blanco, J

    2002-08-01

    Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) catalyses the conversion of p-hydroxy-cinnamaldehydes to the corresponding alcohols and is considered a key enzyme in lignin biosynthesis. By a differential screening of a strawberry (Fragariax ananassa cv. Chandler) fruit specific subtractive cDNA library, a full-length clone corresponding to a cad gene was isolated (Fxacad1). Northern blot and quantitative real time PCR studies indicated that the strawberry Fxacad1 gene is expressed in fruits, runners, leaves, and flowers but not in roots. In addition, the gene presented a differential expression in fruits along the ripening process. Moreover, by screening of a strawberry genomic library a cad gene was isolated (Fxacad2). Similar to that found in other cad genes from higher plants, this strawberry cad gene is structured in five exons and four introns. Southern blot analyses suggest that, probably, a small cad gene family exists in strawberry. RT-PCR studies indicated that only the Fxacad1 gene was expressed in all the fruit ripening stages and vegetative tissues analysed. The Fxacad1 cDNA was expressed in E. coli cells and the corresponding protein was used to raise antibodies against the strawberry CAD polypeptide. The antibodies obtained were used for immunolocalization studies. The results showed that the CAD polypeptide was localized in lignifying cells of all the tissues examined (achenes, fruit receptacles, runners, leaves, pedicels, and flowers). Additionally, the cDNA was also expressed in yeast (Pichia pastoris) as an extracellular protein. The recombinant protein showed activity with the characteristic substrates of CAD enzymes from angiosperms, indicating that the gene cloned corresponds to a CAD protein. PMID:12147722

  7. A lectin with highly potent inhibitory activity toward breast cancer cells from edible tubers of Dioscorea opposita cv. nagaimo.

    PubMed

    Chan, Yau Sang; Ng, Tzi Bun

    2013-01-01

    A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC(50) values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

  8. FANTINA: Fathom Asteroids Now: Tomography and Imagery of a NEA- Payload For Marco Polo R CV3 / ESA mission

    NASA Astrophysics Data System (ADS)

    Herique, A.; Biele, J.; Bousquet, P.; Ciarletti, V.; Ho, T. M.; Issler, J. L.; Kofman, W.; Michel, P.; Plettemeier, D.; Puget, P.; Souyris, J. C.; Ulamec, S.; van Zoest, T.; Zine, S.

    2012-04-01

    The internal structure of small bodies is still poorly known and has never been measured directly. There is no way to determine from ground based observation whether the body is a monolithic piece of rock or a rubble-pile, an aggregate of boulders held together by gravity and how much porosity it contains, both in the form of micro-scale or macro-scale porosity. Knowing this structure is a key point for a better understanding of the asteroid accretion and dynamical evolution. It is the main objective of the FANTINA experience proposed Payload for Marco Polo R CV3 / ESA mission: FANTINA (Marco Polo's Daughter) for Fathom Asteroids Now: Tomography and Imagery of a NEA is to provide measurement capabilities and science data which are not accessible by remote sensing of the asteroid from the Marco-Polo R spacecraft alone and which complement the analysis of the returned samples. The FANTINA payload is a Lander carrying the ASSERT experiment and a complementary payload to be defined: ASSERT is a bistatic CONSERT/Rosetta-like radar to achieve the tomography both in transmission and in reflexion of the asteroid in order to characterise its composition and its heterogeneity from decimetric to global scale. The lander is a long-lived bus in the 10-kg class derived from MASCOT/Hayabusa 2. This paper reviews the science rationale of FANTINA in the context of the Marco Polo R mission to 1996FG3. The surface package concept will be reviewed including the radar tomography principles. So a preliminary design and budget will be done.

  9. Olivine-rich rims surrounding chondrules in the Mokoia CV3 carbonaceous chondrite: Further evidence for parent-body processes

    NASA Astrophysics Data System (ADS)

    Tomeoka, Kazushige; Ohnishi, Ichiro

    2014-07-01

    Fine-grained rims surrounding chondrules and inclusions in the Mokoia CV3 carbonaceous chondrite can be divided into phyllosilicate-rich and olivine-rich types. We present a petrographic and electron microscopic study of the olivine-rich rims and their host objects (referred to as chondrules/olivine-rich rims). The olivine-rich rims consist mainly of Fe-rich olivine and very minor phyllosilicate (saponite). Their host chondrules contain minor saponite and phlogopite, which resulted from aqueous alteration of anhydrous silicates. Mineralogical and compositional characteristics of the chondrules/olivine-rich rims suggest that they experienced mild thermal metamorphic effects. The rims commonly contain veins of coarse-grained Fe-rich olivine, magnetite, and Fe-(Ni) sulfides. The chondrules show abundant evidence of alteration along their peripheries, and the alteration textures suggest a mechanism for rim formation by replacement of the chondrules. Initially, enstatite and opaque nodules preferentially reacted to form coarse, platy, Fe-rich olivine crystals, which were subsequently divided into finer grains. Forsterite was also replaced by Fe-rich olivine. As the alteration advanced, these Fe-rich olivines were disaggregated, mixed with simultaneously produced saponite, and formed rims. In contrast, the surrounding matrix shows no evidence of such alteration and metamorphism. These observations indicate that the chondrules/olivine-rich rims did not experience these secondary processes in their present setting. The results suggest that the chondrules/olivine-rich rims experienced extensive replacement reactions in an environment in which aqueous fluids existed but only in minor amounts. They have probably also undergone simultaneous and/or subsequent mild thermal metamorphism. We suggest that the chondrules/olivine-rich rims are actually clasts transported from a relatively dry region in the parent body that was different from the region where Mokoia was finally lithified.

  10. Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening.

    PubMed

    Dautt-Castro, Mitzuko; Ochoa-Leyva, Adrian; Contreras-Vergara, Carmen A; Pacheco-Sanchez, Magda A; Casas-Flores, Sergio; Sanchez-Flores, Alejandro; Kuhn, David N; Islas-Osuna, Maria A

    2015-01-01

    Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR ? 0.05)]. The expression of 10 genes evaluated by both qRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic process" among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening. PMID:25741352

  11. Photosynthesis and growth responses of mustard (Brassica juncea L. cv Pusa Bold) plants to free air carbon dioxide enrichment (FACE).

    PubMed

    Ruhil, Kamal; Sheeba; Ahmad, Altaf; Iqbal, Muhammad; Tripathy, Baishnab C

    2014-12-01

    Increased atmospheric [CO2] is likely to affect photosynthesis, plant growth, and yield potential of plants. Mustard (Brassica juncea L.) is an important oil seed crop that is widely grown in India. Therefore, the impact of elevated [CO2] (585 ?mol mol(-1)) on pigment and protein content, chlorophyll a fluorescence, photosynthetic electron transport reactions, CO2 assimilation, biomass production, and seed yield potential was measured in B. juncea cv Pusa Bold, grown inside free air carbon dioxide enrichment (FACE) rings installed on the campus of Jawaharlal Nehru University, New Delhi, India. Plants were grown for three consecutive winter seasons (2010-2013), in ambient (385 ?mol mol(-1)) or elevated [CO2], in field conditions. Elevated [CO2] had no significant effect on the minimal chlorophyll fluorescence (F 0), while the quantum efficiency of Photosystem II, measured as variable fluorescence (F v?=?F m-F 0) to maximum fluoresence (F m), increased by 3 %. Electron transport rate, photosystem I, photosystem II, and whole chain electron transport rates increased by 8 % in elevated [CO2]. However, the net photosynthesis rate increased by ?50 % in three growing seasons under elevated [CO2] condition. The stomatal conductance and transpiration rate decreased resulting in higher photosynthetic water use efficiency. The photosynthesizing surface, i.e., leaf area index substantially increased leading to higher biomass and seed yield under elevated [CO2] condition. Acclimatory downregulation of photosynthesis and plant productivity was not observed in three consecutive growing years suggesting that in the absence of nutrient limitation, B. juncea is highly responsive to elevated CO2 whose yield potential shall increase in changing climatic conditions. PMID:25471475

  12. The effects of tropospheric ozone and elevated carbon dioxide on potato (Solanum tuberosum L. cv. Bintje) growth and yield.

    PubMed

    Persson, K; Danielsson, H; Selldén, G; Pleijel, H

    2003-07-01

    An open-top chamber experiment with field grown potato (Solanum tuberosum L. cv. Bintje) was performed in the south-west of Sweden in 1998. The experiment was aimed to investigate individual and interactive impacts of elevated ozone (O(3)) and carbon dioxide (CO(2)) on potato growth and yield. Treatments were ambient and elevated CO(2) with or without the addition of 20 nmol mol(-1) O(3). In addition, plants were grown in ambient air plots. Plants grown in elevated CO(2) and O(3) had a reduced tuber yield, compared to control, by 2% and 8%, respectively. These effects were, however, not statistically significant. Elevated CO(2) caused a significantly larger number of tubers of lower average size, while elevated O(3) induced significantly lower tuber dry matter content. The number of tuber initials (tubers <15 mm) found at harvest was significantly lower in elevated O(3). Visible O(3) leaf injury appeared earlier and the number of yellow leaves 89 days after emergence was significantly larger in elevated O(3). In elevated CO(2), haulm dry weight was significantly (15%) lower at harvest and the haulm/tuber ratio was significantly reduced. There were significant interactive effects of O(3) and CO(2) on the final number of leaves and on stem height. Plants in elevated CO(2) reached final stem height earlier than in the other treatments. In summary, it was concluded: (a) that anticipated future O(3) levels are likely to have a negative impact on potato growth, although tuber yield effects may be hard to demonstrate experimentally in this plant due to large variation; (b) that whether any possible beneficial effect of elevated CO(2) on the magnitude of tuber yield will be expressed, depend on the climatic conditions prevailing; and (c) that potato growth responses to CO(2) and O(3) are not simply additive. PMID:12812743

  13. A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata

    PubMed Central

    Camps, Céline; Kappel, Christian; Lecomte, Pascal; Léon, Céline; Gomès, Eric; Coutos-Thévenot, Pierre; Delrot, Serge

    2010-01-01

    Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays comprising 14?500 probes were used to compare, under three experimental conditions (in vitro, in the greenhouse, and in the vineyard), foliar material of infected symptomatic plants (S+R+), infected asymptomatic plants (S–R+), and healthy plants (S–R–). These plants were characterized by symptom notation after natural (vineyard) or experimental (in vitro and greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative real-time PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (Erysiphe necator, Plasmopara viticola, and Botrytis cinerea). (i) Five functional categories of genes, that is those involved in metabolism, defence reactions, interaction with the environment, transport, and transcription, were up-regulated in S+R+ plants compared with S–R– plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also up-regulated after infection by powdery mildew, downy mildew, and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and the polypeptide fraction secreted by Eutypa. PMID:20190040

  14. A Lectin with Highly Potent Inhibitory Activity toward Breast Cancer Cells from Edible Tubers of Dioscorea opposita cv. Nagaimo

    PubMed Central

    Chan, Yau Sang; Ng, Tzi Bun

    2013-01-01

    A 70-kDa galactose-specific lectin was purified from the tubers of Dioscorea opposita cv. nagaimo. The purification involved three chromatographic steps: anion exchange chromatography on a Q-Sepharose column, FPLC-anion exchange chromatography on a Mono Q column, and FPLC-gel filtration on a Superdex 75 column. The purified nagaimo lectin presented as a single 35-kDa band in reducing SDS-PAGE while it exhibited a 70-kDa single band in non-reducing SDS-PAGE suggesting its dimeric nature. Nagaimo lectin displayed moderate thermostability, retaining full hemagglutinating activity after heating up to 62°C for 30 minutes. It also manifested stability over a wide pH range from pH 2 to 13. Nagaimo lectin was a galactose-specific lectin, as evidenced by binding with galactose and galactose-containing sugars such as lactose and raffinose. The minimum concentration of galactose, lactose and raffinose required to exert an inhibitory effect on hemagglutinating activity of nagaimo lectin was 20 mM, 5 mM and 40 mM, respectively. Nagaimo lectin inhibited the growth of some cancer cell lines including breast cancer MCF7 cells, hepatoma HepG2 cells and nasopharyngeal carcinoma CNE2 cells, with IC50 values of 3.71 µM, 7.12 µM and 19.79 µM, respectively, after 24 hour treatment with nagaimo lectin. The induction of phosphatidylserine externalization and mitochondrial depolarization indicated that nagaimo lectin evoked apoptosis in MCF7 cells. However, the anti-proliferative activity of nagaimo lectin was not blocked by application of galactose, signifying that the activity was not related to the carbohydrate binding specificity of the lectin. PMID:23349827

  15. Control of endogenous bacterial contamination and micropropagation of a traditional table banana (Musa spp. cv. Kanthali) of Bangladesh.

    PubMed

    Titov, Soubir; Bhowmik, Salil Kumar; Alam, Md Sadrul; Uddin, Sarder Nasir

    2007-11-01

    Shoot tips of a traditional table banana (Musa spp. cv. Kanthali) of Bangladesh were evaluated for in vitro propagation. Initial surface sterilization (with 0.1% HgCl2 for 12 minutes) of shoot tips was successful but microbial contamination (mostly bacteria) at the rhizomatous base of the explants was observed within 6-15 days after inoculation which eventually killed 85% of inoculated explants. So, for contamination free culture establishment explants were soaked in two broad spectrum antibiotics namely ampicillin and gentamicin. Cent percent contamination free cultures were established by soaking the explants in 400 mg/L ampicillin or 200 mg/L gentamicin for 1h. Antibiotic treated explants were found to be full contamination free but failed to regenerate after 3 weeks of culture. But some of them absorbed media for up to 2nd subculture and showed swelling of explants and some color changes from pale white to light/deep green. Finally, a few days after 3rd subculture, no growth of explants was observed and all treated explants eventually started to die. Among the untreated alive explants the best medium for single shoot development was MS + 4.0 mg/L BA + 0.5 mg/L KT + 15% CW and average time required for shoot development was 18-21 days. But the regeneration percentage was very low (30%). The best medium for shoot multiplication was MS + 4.0 mg/L BA + 2.0 mg/L IAA + 15% CW and only average 3-4 shoots were formed per shoot. Finally, in vitro proliferated shoots produced roots with maximum frequency (90%) in half strength of MS medium fortified with 0.5 mg/L IBA. PMID:18257234

  16. Structural analysis of wheat wax (Triticum aestivum, c.v. 'Naturastar' L.): from the molecular level to three dimensional crystals.

    PubMed

    Koch, K; Barthlott, W; Koch, S; Hommes, A; Wandelt, K; Mamdouh, W; De-Feyter, S; Broekmann, P

    2006-01-01

    In order to elucidate the self assembly process of plant epicuticular waxes, and the molecular arrangement within the crystals, re-crystallisation of wax platelets was studied on biological and non-biological surfaces. Wax platelets were extracted from the leaf blades of wheat (Triticum aestivum L., c.v. 'Naturastar', Poaceae). Waxes were analysed by gas chromatography (GC) and mass spectrometry (MS). Octacosan-1-ol was found to be the most abundant chemical component of the wax mixture (66 m%) and also the determining compound for the shape of the wax platelets. The electron diffraction pattern showed that both the wax mixture and pure octacosan-1-ol are crystalline. The re-crystallisation of the natural wax mixture and the pure octacosan-1-ol were studied by scanning tunnelling microscopy (STM), atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Crystallisation of wheat waxes and pure octacosano-1-ol on the non polar highly ordered pyrolytic graphite (HOPG) led to the formation of platelet structures similar to those found on the plant surface. In contrast, irregular wax morphologies and flat lying plates were formed on glass, silicon, salt crystals (NaCl) and mica surfaces. Movement of wheat wax through isolated Convallaria majalis cuticles led to typical wax platelets of wheat, arranged in the complex patterns typical for C. majalis. STM of pure octacosan-1-ol monolayers on HOPG showed that the arrangement of the molecules strictly followed the hexagonal structure of the substrate crystal. Re-crystallisation of wheat waxes on non-polar crystalline HOPG substrate showed that technical surfaces could be used to generate microstructured, biomimetic surfaces. AFM and SEM studies proved that a template effect of the substrate determined the orientation of the re-grown crystals. These effects of the structure and polarity of the substrate on the morphology of the epicuticular waxes are relevant for understanding interactions between biological as well as technical surfaces and waxes. PMID:16133211

  17. Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma)

    SciTech Connect

    Lynch, D.V.; Steponkus, P.L. (Cornell Univ., Ithaca, NY (USA))

    1987-01-01

    Highly enriched plasma membrane fractions were isolated from leaves of nonacclimated (NA) and acclimated (ACC) rye (Secale cereale L. cv Puma) seedlings. Collectively, free sterols, steryl glucosides, and acylated steryl glucosides constituted > 50 mole % of the total lipid in both NA and ACC plasma membrane fractions. Glucocerebrosides containing hydroxy fatty acids constituted the major glycolipid class of the plasma membrane, accounting for 16 mole % of the total lipid. Phospholipids, primarily phosphatidylcholine and phosphatidylethanolamine with lesser amounts of phosphatidylglycerol, phosphatidic acid, phosphatidylserine, and phosphatidylinositol, comprised only 32 mole% of the total lipid in NA samples. Following cold acclimation, free sterols increased from 33 to 44 mole %, while steryl glucosides and acylated steryl glucosides decreased from 15 to 6 mole % and 4 to 1 mole %, respectively. Sterol analyses of these lipid classes demonstrated that free {beta}-sitosterol increased from 21 to 32 mole % (accounting for the increase in free sterols as a class) at the expense of sterol derivatives containing {beta}-sitosterol. Glucocerebrosides decreased from 16 to 7 mole % of the total lipid following cold acclimation. In addition, the relative proportions of associated hydroxy fatty acids, including 22:0 (h), 24:0 (h), 22:1 (h), and 24:1 (h) were altered. The phospholipid content of the plasma membrane fraction increased to 42 mole % of the total lipid following cold acclimation. Although the relative proportions of the individual phospholipids did not change appreciably after cold acclimation, there were substantial differences in the molecular species. Di-unsaturated molecular species of phosphatidylcholine and phosphatidylethanolamine increased following acclimation. These results demonstrate that cold acclimation results in substantial changes in the lipid composition of the plasma membrane.

  18. Hudiburg CV Tara Hudiburg

    E-print Network

    DeLucia, Evan H.

    Forests · Advisor ­ Professor Beverly Law B.S. Biology (1998), Pacific Lutheran University, Tacoma, WA, Pacific Lutheran University Courses: Principles of Biology core sequence, Field Methods of Ecology #12;L

  19. John Cushman's CV

    E-print Network

    F. Alejandro Bonilla

    Society for Industrial and Applied Math; American Geophysical Union (Fellow); Society of Engineering Science; Soil Science Society of America (Fellow); Materials ... chaotic dynamics in fractal porous media with application to microbe motility. ..... Cushman, J.H. (1985) Multiphase Transport Based on Compact Distributions.

  20. Xu Zhang's Homepage - CV

    E-print Network

    T. Lin, Y. Lin and X. Zhang, A Method of Lines Based on Immersed Finite ... Element Methods and Application to Plate Bending Problems, Appl. Math. ... 8, International Congress on Industrial and Applied Mathematics, Beijing, China. ... 3, SIAM Conference on Computational Science and Engineering, Salt Lake City, UT.